LOCUS       DAVKNL010000001        74067 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVKNL010000001 DAVKNL010000000
VERSION     DAVKNL010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 74067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74067
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.001"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(8..1237)
                     /locus_tag="VW411_000001"
     CDS             complement(8..1237)
                     /locus_tag="VW411_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU9792820.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLAL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILELQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEYYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(1227..2393)
                     /locus_tag="VW411_000002"
     CDS             complement(1227..2393)
                     /locus_tag="VW411_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU9792821.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            2537..3550
                     /locus_tag="VW411_000003"
     CDS             2537..3550
                     /locus_tag="VW411_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEU9792822.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(3595..4014)
                     /locus_tag="VW411_000004"
     CDS             complement(3595..4014)
                     /locus_tag="VW411_000004"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEU9792823.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(4025..5431)
                     /gene="atpD"
                     /locus_tag="VW411_000005"
     CDS             complement(4025..5431)
                     /gene="atpD"
                     /locus_tag="VW411_000005"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU9792824.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFAGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(5517..6395)
                     /locus_tag="VW411_000006"
     CDS             complement(5517..6395)
                     /locus_tag="VW411_000006"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEU9792825.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLSDQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(6411..7916)
                     /locus_tag="VW411_000007"
     CDS             complement(6411..7916)
                     /locus_tag="VW411_000007"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU9792826.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYAGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(7931..8467)
                     /locus_tag="VW411_000008"
     CDS             complement(7931..8467)
                     /locus_tag="VW411_000008"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEU9792827.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(8467..8961)
                     /gene="atpF"
                     /locus_tag="VW411_000009"
     CDS             complement(8467..8961)
                     /gene="atpF"
                     /locus_tag="VW411_000009"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEU9792828.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(8975..9691)
                     /gene="atpB"
                     /locus_tag="VW411_000010"
     CDS             complement(8975..9691)
                     /gene="atpB"
                     /locus_tag="VW411_000010"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEU9792829.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(9726..9926)
                     /locus_tag="VW411_000011"
     CDS             complement(9726..9926)
                     /locus_tag="VW411_000011"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEU9792830.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            complement(10001..11007)
                     /locus_tag="VW411_000012"
                     /pseudo
     CDS             complement(10001..11007)
                     /locus_tag="VW411_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(11452..11850)
                     /locus_tag="VW411_000013"
     CDS             complement(11452..11850)
                     /locus_tag="VW411_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9792831.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDCLYIRQRYYYQKNL"
     gene            complement(12358..12840)
                     /gene="greA"
                     /locus_tag="VW411_000014"
     CDS             complement(12358..12840)
                     /gene="greA"
                     /locus_tag="VW411_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEU9792832.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFVGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            complement(12911..14566)
                     /gene="mltG"
                     /locus_tag="VW411_000015"
     CDS             complement(12911..14566)
                     /gene="mltG"
                     /locus_tag="VW411_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU9792833.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSTEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     IRDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAEAF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            complement(14647..15141)
                     /locus_tag="VW411_000016"
     CDS             complement(14647..15141)
                     /locus_tag="VW411_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9792834.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            complement(15138..15602)
                     /locus_tag="VW411_000017"
     CDS             complement(15138..15602)
                     /locus_tag="VW411_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9792835.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQCLKVHSLVIEFDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            complement(15612..16946)
                     /locus_tag="VW411_000018"
     CDS             complement(15612..16946)
                     /locus_tag="VW411_000018"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEU9792836.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSITGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            complement(16957..17574)
                     /locus_tag="VW411_000019"
     CDS             complement(16957..17574)
                     /locus_tag="VW411_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU9792837.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVKPIEKQVEKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTAPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            complement(17622..20720)
                     /locus_tag="VW411_000020"
     CDS             complement(17622..20720)
                     /locus_tag="VW411_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9792838.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQNIVQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQVRKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPLQATQVKADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVTVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            complement(20813..21979)
                     /locus_tag="VW411_000021"
     CDS             complement(20813..21979)
                     /locus_tag="VW411_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9792839.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDSLFEIDFDQLEKDLVEEE
                     VKLYVLCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVMDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
     gene            complement(21987..23081)
                     /locus_tag="VW411_000022"
     CDS             complement(21987..23081)
                     /locus_tag="VW411_000022"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU9792840.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGHFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDAGDLIADLRQAIEG"
     gene            complement(23239..24360)
                     /locus_tag="VW411_000023"
     CDS             complement(23239..24360)
                     /locus_tag="VW411_000023"
                     /inference="COORDINATES: protein motif:HMM:NF012869.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9792841.1"
                     /translation="MTEKIQEHELIKTNQAEKSVQDVLDNCIERVQNNSLKSDRVTSF
                     ETPFALLFIFATIAVMLTYGGYRVSAGYISVGTLVSFLIYLFQLLNPISNIANFVTVY
                     SRSKGSSVALENLLAVPKEKFEGGKSVSGRGLNFNHVYFGYDENRPVLKDITCSIFKG
                     QKIAFVGPSGSGKSTIVRLLERFYKPLSGDILMEQSSIYDFNLKEWRSKIAWVSQNNA
                     VLSGSIRDNLCLGLNRLVTDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRLLSGGR
                     SQRLQIARAYLKDAEILIFDEATANLDADSEYAIISSLYSVLKEKTVVIIAHRLSTVK
                     DVDCIFFLEEGKITGSGTHKELLENHERYARFVQEQMIE"
     gene            complement(24369..24914)
                     /locus_tag="VW411_000024"
     CDS             complement(24369..24914)
                     /locus_tag="VW411_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9792842.1"
                     /translation="MKLKLLRVDTKVIMGSFLLVLSSLLALLLPLILKDLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKKIAKIRKKVIEGLIYVEKSFFDKSQS
                     GELTSAIVNDTSVIREFLITTFPNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMM
                     FIILPLSNISEKYSRRLQEEI"
     gene            complement(25086..27044)
                     /locus_tag="VW411_000025"
     CDS             complement(25086..27044)
                     /locus_tag="VW411_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9792843.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGFLQNLGLEPGEANDKAKAVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            complement(27247..28869)
                     /locus_tag="VW411_000026"
     CDS             complement(27247..28869)
                     /locus_tag="VW411_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU9792844.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRIFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            28975..30420
                     /locus_tag="VW411_000027"
     CDS             28975..30420
                     /locus_tag="VW411_000027"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEU9792845.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVIFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVKRVYGLTFDVGVFLNISPDHIGPIE
                     HPSFEDYFYHKRLLMEKSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            complement(30463..30660)
                     /locus_tag="VW411_000028"
     CDS             complement(30463..30660)
                     /locus_tag="VW411_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU9792846.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTKE"
     gene            complement(30761..31369)
                     /locus_tag="VW411_000029"
                     /pseudo
     CDS             complement(30761..31369)
                     /locus_tag="VW411_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
     gene            complement(31394..31906)
                     /locus_tag="VW411_000030"
     CDS             complement(31394..31906)
                     /locus_tag="VW411_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEU9792847.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACKHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            complement(31992..32927)
                     /locus_tag="VW411_000031"
     CDS             complement(31992..32927)
                     /locus_tag="VW411_000031"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEU9792848.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            complement(32998..33297)
                     /locus_tag="VW411_000032"
     CDS             complement(32998..33297)
                     /locus_tag="VW411_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEU9792849.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            complement(33287..34036)
                     /locus_tag="VW411_000033"
     CDS             complement(33287..34036)
                     /locus_tag="VW411_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEU9792850.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDLCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVNPYSNLNESEHYLLARHEITTNLEEICRSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            complement(34090..34449)
                     /locus_tag="VW411_000034"
     CDS             complement(34090..34449)
                     /locus_tag="VW411_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEU9792851.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            complement(34451..35851)
                     /locus_tag="VW411_000035"
     CDS             complement(34451..35851)
                     /locus_tag="VW411_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEU9792852.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFAALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            complement(36146..36385)
                     /locus_tag="VW411_000036"
     CDS             complement(36146..36385)
                     /locus_tag="VW411_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEU9792853.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            complement(36417..36887)
                     /gene="ssbA"
                     /locus_tag="VW411_000037"
     CDS             complement(36417..36887)
                     /gene="ssbA"
                     /locus_tag="VW411_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEU9792854.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            complement(36899..37189)
                     /locus_tag="VW411_000038"
     CDS             complement(36899..37189)
                     /locus_tag="VW411_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU9792855.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            complement(37342..38688)
                     /gene="asnS"
                     /locus_tag="VW411_000039"
     CDS             complement(37342..38688)
                     /gene="asnS"
                     /locus_tag="VW411_000039"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEU9792856.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            complement(38704..38994)
                     /locus_tag="VW411_000040"
     CDS             complement(38704..38994)
                     /locus_tag="VW411_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9792857.1"
                     /translation="MDYKLAFKTSSLISFLSPNSPHPGGDFWLTQGTQDPVHFAFLAE
                     NKEEVQACYEAGLEAGGRDNGAPGYRSEHPIYYAAFMIDLDGNNIEVVCHKE"
     gene            complement(39062..40249)
                     /locus_tag="VW411_000041"
     CDS             complement(39062..40249)
                     /locus_tag="VW411_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9792858.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTVILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            complement(40246..40734)
                     /locus_tag="VW411_000042"
     CDS             complement(40246..40734)
                     /locus_tag="VW411_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEU9792859.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISGSFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKITFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            41055..41603
                     /locus_tag="VW411_000043"
     CDS             41055..41603
                     /locus_tag="VW411_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9792860.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIK"
     gene            complement(41880..42608)
                     /gene="yaaA"
                     /locus_tag="VW411_000044"
     CDS             complement(41880..42608)
                     /gene="yaaA"
                     /locus_tag="VW411_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEU9792861.1"
                     /translation="MKILIPTAKEMNTDFPSIEAIPLKPESQAVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPVLSPMAPHRLDFLMKLKVAGKTLKSHWKAAYDETLKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(42687..42851)
                     /locus_tag="VW411_000045"
     CDS             complement(42687..42851)
                     /locus_tag="VW411_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEU9792862.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            complement(42918..44513)
                     /locus_tag="VW411_000046"
     CDS             complement(42918..44513)
                     /locus_tag="VW411_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9792863.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTN
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESINGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKLR"
     gene            complement(44525..44935)
                     /locus_tag="VW411_000047"
     CDS             complement(44525..44935)
                     /locus_tag="VW411_000047"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU9792864.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            complement(45051..45842)
                     /gene="yghU"
                     /locus_tag="VW411_000048"
     CDS             complement(45051..45842)
                     /gene="yghU"
                     /locus_tag="VW411_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEU9792865.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            complement(45855..48551)
                     /locus_tag="VW411_000049"
     CDS             complement(45855..48551)
                     /locus_tag="VW411_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEU9792866.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIRTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            48855..50039
                     /gene="mntE"
                     /locus_tag="VW411_000050"
     CDS             48855..50039
                     /gene="mntE"
                     /locus_tag="VW411_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEU9792867.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSNALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            complement(50182..52053)
                     /locus_tag="VW411_000051"
     CDS             complement(50182..52053)
                     /locus_tag="VW411_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9792868.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELDRAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQDVSFAYENKPILQN
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTAGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(52050..53234)
                     /locus_tag="VW411_000052"
     CDS             complement(52050..53234)
                     /locus_tag="VW411_000052"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEU9792869.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGGILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            complement(53246..54013)
                     /locus_tag="VW411_000053"
     CDS             complement(53246..54013)
                     /locus_tag="VW411_000053"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEU9792870.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            complement(54290..55138)
                     /locus_tag="VW411_000054"
     CDS             complement(54290..55138)
                     /locus_tag="VW411_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9792871.1"
                     /translation="MKLAVFTDSSAYLSAEILQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            complement(55140..55514)
                     /locus_tag="VW411_000055"
     CDS             complement(55140..55514)
                     /locus_tag="VW411_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEU9792872.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            complement(55588..56940)
                     /locus_tag="VW411_000056"
     CDS             complement(55588..56940)
                     /locus_tag="VW411_000056"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU9792873.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVNYYVDTITDVVRAEIGID
                     "
     gene            complement(56964..57743)
                     /locus_tag="VW411_000057"
     CDS             complement(56964..57743)
                     /locus_tag="VW411_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEU9792874.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            complement(57730..58587)
                     /locus_tag="VW411_000058"
     CDS             complement(57730..58587)
                     /locus_tag="VW411_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEU9792875.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            58723..59688
                     /locus_tag="VW411_000059"
     CDS             58723..59688
                     /locus_tag="VW411_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU9792876.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEFAITTSK
                     GSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSAV
                     DWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKLE
                     ITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYAI
                     GDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            complement(59722..60633)
                     /gene="whiA"
                     /locus_tag="VW411_000060"
     CDS             complement(59722..60633)
                     /gene="whiA"
                     /locus_tag="VW411_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEU9792877.1"
                     /translation="MSFTVAVKEEILGQHHLSWHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHLYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(60630..61607)
                     /locus_tag="VW411_000061"
     CDS             complement(60630..61607)
                     /locus_tag="VW411_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEU9792878.1"
                     /translation="MRKPKITVIGGGTGSPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            complement(61604..62494)
                     /gene="rapZ"
                     /locus_tag="VW411_000062"
     CDS             complement(61604..62494)
                     /gene="rapZ"
                     /locus_tag="VW411_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEU9792879.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            complement(62546..62926)
                     /locus_tag="VW411_000063"
     CDS             complement(62546..62926)
                     /locus_tag="VW411_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU9792880.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            complement(62937..63524)
                     /locus_tag="VW411_000064"
     CDS             complement(62937..63524)
                     /locus_tag="VW411_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEU9792881.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            complement(63533..64765)
                     /gene="clpX"
                     /locus_tag="VW411_000065"
     CDS             complement(63533..64765)
                     /gene="clpX"
                     /locus_tag="VW411_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEU9792882.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            complement(64967..65473)
                     /locus_tag="VW411_000066"
     CDS             complement(64967..65473)
                     /locus_tag="VW411_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEU9792883.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            complement(65603..66121)
                     /locus_tag="VW411_000067"
     CDS             complement(65603..66121)
                     /locus_tag="VW411_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEU9792884.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            complement(66617..68121)
                     /gene="lytC"
                     /locus_tag="VW411_000068"
                     /pseudo
     CDS             complement(66617..68121)
                     /gene="lytC"
                     /locus_tag="VW411_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
     gene            complement(68159..68917)
                     /locus_tag="VW411_000069"
     CDS             complement(68159..68917)
                     /locus_tag="VW411_000069"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU9792885.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            complement(69015..69692)
                     /locus_tag="VW411_000070"
     CDS             complement(69015..69692)
                     /locus_tag="VW411_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEU9792886.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFIN
                     AMDLWKD"
     gene            complement(69701..70645)
                     /locus_tag="VW411_000071"
     CDS             complement(69701..70645)
                     /locus_tag="VW411_000071"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEU9792887.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            complement(70827..71339)
                     /locus_tag="VW411_000072"
     CDS             complement(70827..71339)
                     /locus_tag="VW411_000072"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEU9792888.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            complement(71426..72184)
                     /locus_tag="VW411_000073"
     CDS             complement(71426..72184)
                     /locus_tag="VW411_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU9792889.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            72392..72538
                     /locus_tag="VW411_000074"
     CDS             72392..72538
                     /locus_tag="VW411_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9792890.1"
                     /translation="MEIKEQTRKLAVSCSKYIFEVADKTDEISNHTYGKATLTWFEEI
                     FEEY"
     gene            complement(72685..73815)
                     /gene="ugpC"
                     /locus_tag="VW411_000075"
     CDS             complement(72685..73815)
                     /gene="ugpC"
                     /locus_tag="VW411_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEU9792891.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFITVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
BASE COUNT        21508 a        16757 c        13776 g        22026 t
ORIGIN      
        1 aacattctta caatctctca aacagttctt gcatttgcac atcatctgga accagtttta
       61 agtaagtgtg agcatggact tttgcttctt caaaatgtcc caattcacgc aagagataga
      121 tatagtgttc cagaaattct ggattgtcct tcaaatctcc tgtcaactct tgataatact
      181 cataagcagt atccaaatcg tccatttctt gataagaacg agcaatcatc cacttggtca
      241 aaagattttc tggctcctca ctctgcaatt ctagaatatc ctcataacgc tcctgctcca
      301 gataaatagt ggctaaacga agcaagattt cttctgtatc ctcagcgtct tcttttgcag
      361 taaggagata attttctgca ccactagcat catgcaattc ataagaaaat tgtgaagcag
      421 ctagcaagag gcgagtttca aagggatttt tctctaatcc ttgcttagcg atacgcaggg
      481 cttcttgaac ttgatgttcc ttatgtaaag cctgactgta cccatactca tagccttcaa
      541 agtcaggaga aatggtatca agctgcttaa agtagagggt ggctttttga tattcttctt
      601 gatcaaaata aagactggcc aactcaaaag ctgttaagtc atcgtattct aactccaggg
      661 ctttttctaa aaactcagta gccgtttcaa atttccctaa ctgagcatag gcaaagccaa
      721 ttcgttgata ggtggaaatg cccgtttgct cataaatcga gcgattatct aactgggcat
      781 aggcttgaat agccgcttgg taattttcca actcactatc caactctgcc aaacccaata
      841 tcaagagaga atcctctgag taggtcaagg cctccaataa tttctcacgt gccacatctg
      901 tcaaaccttc cagctggtaa aggtctgcct tcagagccaa agacgagaca taccagtcac
      961 tgtcagcttg gatttcctca agataggtaa aggcttcttc tatttgacca tcctcgctag
     1021 caattgcagc tagattaaga tgaacctctg gaaaatcctc tacaattttc aggtaaattt
     1081 ccttggcctg aggatagaaa ccaatccctt caagataagt tgccaattca tacagaagat
     1141 cacttgaatc attttctaaa gctttggcga aataatgctc agccttagtt aaatcttgct
     1201 cctccaaagc ctgtaacatc tgttgactat tgttcactct tgacttcctc accctctaat
     1261 tcatatagac cactgactac cttataccat tcgaaaatgg ctgaaatgac aaccttagca
     1321 gaggcataaa ccggaatacc aagtaaaact ccccagatac caaacataga tcctgaagtt
     1381 aacaaaacaa agagaacatt aatagggtgg atgtttaatt gacttcccaa aatcaatgga
     1441 gagacaaaac ggccttcaat agtttgttct acgataaaga caatcactac tttcaaaagc
     1501 atgactggac cagcaatcaa acccaatact agagcaggaa gcatggctag aaagctacca
     1561 agataaggga ccagatttaa aataccagca gtaaccccca gcgtaaccgc atagcgtaga
     1621 ccaataatct tgaagaagat gataaacatt actgctacaa taatagccac tgtcacttgc
     1681 cctcgaacat agttggacaa ctgttgattc acatctgata aaacttgtcc aacaggttcc
     1741 ttcaattttc ttggaatgaa ttgggtcaaa tagttacgca agcctttccc atcacgcaag
     1801 agataaaaga gcatgaaagg aacgataatc aaggcaacaa tcacttgaga agccccgcta
     1861 ataaaggcac tcacccagtt gactgcctga gatgaaacct tacttgccaa aactgtagcc
     1921 tggctagaaa aattggtcaa aacttgctct aattgaggtc tgaaatcatc tggcaggtgc
     1981 tgggctacca atccattaac aatcctatct atatcttcta agtaaacagg aacgtttctt
     2041 gcaaaggtca aaacctgacg ttgcagattt ggaatggcga ctgccaagcc ccaaatgata
     2101 aagagagcga tgataacaaa gacaatagtg atagctataa cacgattaac cttatgcttc
     2161 tccatccaat caacaatagg attcaacaaa taatataaca aaccagacaa aatgactggc
     2221 aacatcacaa ctgctaaaaa gtctaaaaca ggtgaaaata gaaaactaat cttacttaaa
     2281 ataaaaagat tcagtcccaa taataaggtt actaaaaata ccgtaattgc cttgttatct
     2341 aaaaaccact tgaaaaacca agataggcta aaatgtttct ctttttgctc cataaatact
     2401 tcctttctat tgttctctca ttataccatt tttgaaacaa aataactctt ttttaaagtg
     2461 cttacatcta attggatatc tttaatttac ttgtttttgt gatataatag tcttatcttt
     2521 acacagaggt atagtaatga aagaaactgt ttattttgga acttatacac gtcgtacttc
     2581 tcaagggatt tacaaggcag actttgatac agaaactggt cagctttcaa atctagaact
     2641 ttttgcagct gagccaagtc caacctacct tgcctttgac cagcaccaac atttatacac
     2701 tgttggtagc caagacgata aggggggaat tgcagcctat caaactgacg ggactgtgtt
     2761 aaatcatgtt gttgaagaag gagctcccca ctgttatgtt gctgtcgatg aaaagcgtga
     2821 tttggtttac gcagctaact atcacaaggg acaagtcctt gtttataaac gccaggaaga
     2881 tggtagtctt ctacttagtg atatggatca acacagtggc caaggtccac atgaaaatca
     2941 agcttccccc catgttcact atacagattt aacacctgac cactatctag tgacctgcga
     3001 cttgggaact gaccaagtca tcacctatga cctcgatcaa gaaggaaaat tatctaagct
     3061 ctatacctat cacagcaagc caggagcagg ctcacgccat atcattttcc ataaccacta
     3121 taaaatcgct tatctcattt gtgaactcaa tagtactatc gaagttttaa tctacgatgg
     3181 cgttggcgaa tttgaacgta tgcaggtcat ttcaacttta ccggaagctt acgaaggctt
     3241 taatggaacc gctgctattc atctctctaa agacggtaaa tacctctacg cttctaaccg
     3301 tggccatgat tctatcgcag tatataccat ccttgcggac ggtagcttag agttattaga
     3361 aatcgttcca acgcatggtc agactccacg tgattttgat ttgacacccg accaaaaatt
     3421 tctcattgtt gtccatcaag actctgacaa tgcaactgtc tttaaacgta attgtgacaa
     3481 tggtcgtcta gcagaactct ccaacgactt ccatgttccc gaagcagtct gcatccgttt
     3541 tgctccttaa aacaccataa aaaatgaact tggtattttc aagttcattt tttcttatag
     3601 tctatttccg acattaatac ggttaatagc acgttgcaaa gcaatcttag cacgacgttc
     3661 ttggtcaatc aaatgtttgt cttgtgcttc ttcaattgca cgttctgcac gaagtttggc
     3721 acgttctgca cgactgatat cgatatcacg agcacgttct gcagagtcag cgacgattgt
     3781 gatcatatca ttggcaattt caataacgcc accgtttact gcaatccagt tcacgtgatc
     3841 tttatcatcg atacgtttta cctttacttc atcaactgct aaaaccgcaa tcatattttc
     3901 atgtcgtggc aagatcccca tctcaccatc cagagttcga accgatacat agctggcatg
     3961 gtgatcatag acgagaccat ctggtgtcac gatctggaca gttaactgag ccatagatca
     4021 cctcttaaaa tcccattttt tcagcttttg caatcacatc ttcgatagaa cctacaccac
     4081 ggaaggcatc ttctggcaag tggtcgtatt taccatcaag gatttcctta aagccacgta
     4141 cagtttcagc aactggaaca taagaacctg gctgaccagt aaattgttcc gcaacgttga
     4201 agttttgtga caagaagaac tggatacgac gggcgcgagc aaccaaggtc ttttcttcat
     4261 cagaaagctc atccatacca aggatagcaa tgatatcttg caattcatgg taacgttgaa
     4321 ggacacgttt tacttcagca gcaactgcat agtgctcttc tccaacgatt tcaggtgcca
     4381 aggcacgtga gcttgaagca agtgggtcaa cggctgggta gatacccaat tgtaccaact
     4441 tacgttccaa gtttgttgtt gaatccaagt gagcgaaggc tgttgctggc gctgggtcag
     4501 tatagtcatc cgctggcaca tagatagcct ggatagaggt tacagaaccc ttcttggttg
     4561 atgtgatacg ttcttgcaat tgacccattt ccgtagcaag tgttggttgg taaccaacgg
     4621 ctgatggcat acgacccaaa agggcagata cttctgaacc agcctgagtg aaacggaaga
     4681 tattatcgat aaagagaagc acgtcttggc cttccacatc acggaagtat tcagcgattg
     4741 tcaaaccagt aagggcaaca cgcatacgtg ctcctggtgg ctcattcatc tgaccaaaga
     4801 ccatggctgt tttctcgata acgcctgatt ctttcatttc ccagtaaagg tcattcccct
     4861 cacgagtacg ttccccaaca ccagcaaata ctgaaatacc accgtgctct tgggcaatgt
     4921 tgtgaatcaa ttcttggatt aagacagttt taccaactcc ggcaccaccg aaaagtccaa
     4981 ctttaccacc tttaaggtaa ggggcaagaa ggtcaataac cttgatccct gtttcaagga
     5041 tttcagaaga ggtagacaac tcatcaaaag ttggagcttt tttatgaatt ggctgacgct
     5101 ctgcgtcttc tgtaaaagga gcttccaagt caatggtatc tcccaaaacg ttgaagacac
     5161 gtcccaaagt ttctttacct actggtacag agattggacg acctgtgtcc aatacttcca
     5221 ttccacgagt caacccatct gttgattcca tggcgatagt acgaaccata ccatctccta
     5281 actccaaggc tacttcaagg acgatttttg tttttctttc gtcatttttg tagacgacaa
     5341 gtgcattgtt aatctcagga agtttttccc ctgctgcaaa caaaacgtct acaacgggac
     5401 cgataacctg agcaatttta cctgaactca tctccttctc ctattctata taagatactg
     5461 tcggttccta gctcaactag gtcctaagtt cattttcata cgagctggac tagagcctat
     5521 tctaaggcac tagcacctgc tacgatttct gtaatttctt gtgtaatcgc cgcctgtctg
     5581 gcacggttat actgaattgt caaatcattg atgactttct tagcattatc tgtcgctgtt
     5641 tgcatggctg tcatgcccgc agcattctca gctgtcttgg catcgataat ggcaccgtaa
     5701 atcatacttt ctgcaaactg aggcaacaac tgctccagaa tttcttctcg gctggtttcc
     5761 aattcaaaag tcaagctgta ctcttcatcc gcttcatttg gatccaagtc aacaatcgga
     5821 agcatttgtt ccacacgcat ttgactggtt agcgtattga catggtggtt gtagcaaaca
     5881 taaagctcat caaagagttc attttggtac atttcaacag tttttgaaat aatcttacga
     5941 acttgatcaa agctaggttg gtctgacaag ccacgtaatt cataaagtgg ttgaataccg
     6001 cgagccttaa agaaatcagc tcccatccca ccgatacaga tcatttcaaa acctttaccg
     6061 tctgggtggt attcttcttt caactccata acagctttca aaatagagga attataacct
     6121 ccaaccaaac cgcggtctga agtgataacg atatagcctg tcttcttcac agaacggcta
     6181 atcaacatcg gattagttga agcaccagct ccattaccat gaaggatatc tgtcaaaagt
     6241 ttacgcactt tctgagcgta aacttggaag ttgcgagcag cttcttcaga acgacctagc
     6301 ttagcagccg ataccatttg catggcatta gtgatttgac tcgtattttt tgttgaggcg
     6361 atttttgttt taatatcatt tagagatact gccatctgac acctctattc ttattggaag
     6421 ctagattgat tgagaaactc tgtaatcgca gcatccaaga ctgcttcttc tggcaagtct
     6481 tttgtatcac gaatggtttc caaaatctct ggatgttgag catcaaagaa ggcatggaac
     6541 tcttcctcga aacgaacaat atcatctact ggaacagtat ccaagaaacc atgtgtcaaa
     6601 gcataaagaa tggttacttg tttctcaaca ggtaatggtt tgtgaacagg ttgtttcaag
     6661 acctcaacgg tacgacgtcc acggttcaac ttagcctgtg ttgctgcgtc caagtcagaa
     6721 ccaaacttag taaaggcttc caactcacgg tatgaagcaa ggtcgatacg aagtgtacca
     6781 gcaaccttct tcatggcttt gatttgtgca gaaccaccta cacgagatac agatgaaccc
     6841 gcatcgatgg ctggacgaat acctgcattg aagaggccat cgccaaggaa gatttgtcca
     6901 tcagtgatag aaatcacgtt ggttgcgata taggctgaga tatctcctgc ttgtgtctcg
     6961 ataaatggta gggctgtaat tgatccacca ccaagttcat cagaaacttt agctgagcgc
     7021 tcaagcaaac ggctgtggag atagaaaaca tcccctggga aggcttcacg acctggagga
     7081 cgacgaagca agagcgacag ttcacgataa gctaccgctt gttttgatag atcatcgtat
     7141 acaatcaaaa catgcttacc ttgatacata aattcttccg ccatagcaac cccagcataa
     7201 ggagctagga agagcaatgg agatggttgt gaagcagagg ctgtcacaac gattgtgtag
     7261 tccaaggcac cgtactgacg aagtgtttct acttgcgtac gaactgttga ttctttttgt
     7321 ccaatcgcga cgtagataca gatcatatct tgatcttttt ggttcaagat tgtatcaatc
     7381 gcaatggttg ttttccctgt ctgacggtca ccgataatca actcacgttg accacgacca
     7441 atcggtacaa gggcgtcaat agctttcaaa ccagtttgca atggttctga aacagactta
     7501 cgttgcataa caccaggagc tggtgcttct actggacgag ttttatcagt gtggatttct
     7561 ccaagaccgt caactggacg accaagcgga tccacaacac gaccaatcag actttcacct
     7621 acagggactt ccatgatttt ccctgtacgg cggattgtat cgccttcacg gatatctgta
     7681 aagtcaccta ggatgataat accaacgtct gttgactcca agttttgagc cataccataa
     7741 gagccgtttt caaaattcaa caactctcca ctcatgacat tttcaaggcc gtgagcacgc
     7801 gcgataccgt ccccgatata ggttacaaca cctgtttcag tcacatcaaa attgggtttg
     7861 aaattttcaa tttgttgctt aattaaagcg ctgatttctt gtgcgttaat tgccaaaaga
     7921 acaccacttt ctatttcaaa ttttctttaa caactttaag ttgttgttta atactcacat
     7981 caattgtctt gtgattggca aaaatgacaa aaccaccaat gagactttca tcgatttgtt
     8041 cttttacact ccttactttc agagacattt ttttctcaat caaagggagc aaacgagtct
     8101 tctgttcatc agttagagga tgagcagacg taatcgtcac ttcaaatcga tttgtttctt
     8161 tttcaagtcg gttcaagcaa tctacaagca catcataaaa aagatttgct ctgtgattgt
     8221 aggccagaac ctggataaag ttttgtaata aaggcgacac agaatcttgg aaaaaagcaa
     8281 ttgttttttc cttatccgac tcgtctactg ccacttgttt taaaaaagaa ggcagacctg
     8341 ttttttcaac aacttgcttg atttgagtca agtctgaaaa gatacggtct tcttctcctt
     8401 tttcaagtac caattggaca aaaggcatgc tgtatttttc aattaccttt actgttttct
     8461 tgtccattaa gcttctccta gctgatcgat atactgatca atgagtgctt tatgggcatg
     8521 actgtcaagg ttttgtgaga tgattttacc agctaagctg atggtcaaat ctgcgacctc
     8581 acccttaaca ctctgtaaag cttctacttt attttgagca atttcttgat tggctttttc
     8641 ttttaagtgt cctgcttcta gtttagcatc tgctaagata ttagccttac tttgctcagc
     8701 tgtttccttt gcattttcaa tgattgtctt agcttcttta cggctaccag ccaattcatc
     8761 ttcgcgtttt tgagccaata cttctgcttt ttgacgggct tcttcagctc tgtcaatatc
     8821 tgaagcaatt ttttcagctc tttcttcgaa aatgcctgta atattagacc atgcaaattt
     8881 tttaatcaag actagcaaaa gaataaaaga gccagtgatt aaaataaaat taccaattaa
     8941 ttcacctact gttacgtgca tcagttactc ctttctattc ctcttcaata ttaatcttat
     9001 tccctaaata cacagatgtc aaaagagtaa aaacataagc ttggatgcag gaaataaaga
     9061 cagaaaatgc agtccaagcc aaattagctc caaaggctac tggataccaa taaatagctt
     9121 ggtgggaaag aagaagtaac aaactcgtca tgacctctcc tgcaaagata ttcccaaaaa
     9181 tccgcaaagc caaagataag aagtttgtaa attcttccaa gatattcatc ggtatgacaa
     9241 aaacaggact cataaaagat tttatacttt ttttaaatcc acgacgacga acgctttcta
     9301 tatgtgtcaa caaaatgaca agaaaagata aggttaagtc atactgtaaa ttagcggttg
     9361 gcgaactcca ccagttagtc ccatcgatcg tttgaagctt tgtcattaag ccaaggttat
     9421 tggcaatcac catgaaaagg aataaacaaa ggaaaaagag tgagtaatct ttcatgtagc
     9481 gcgaaccaat gttaggttct gtaaatccaa taacaaagtc atagacatac tcaagtacat
     9541 tttgctttcc tttgggtttc aaggtcatat tgcggcttgc ccaataaata aagacaaaaa
     9601 taactcccac aatcaacaaa gtcatggcta acatagtcag attgaagata acaggaccaa
     9661 tagagatgat tggattaata ctttcttcca tctaaaatcc tcccttttct tctcgttcca
     9721 tgtatttatt tgataataaa tgagaagaca agagttacaa agaaagttcc ttcaataaag
     9781 gcaacaccta aaaacatcaa actacgaaac tcagaaagca tatctggttg gcgtgctact
     9841 gatttaaaca gtccattcat caataaacct tcaccgacag atacgcccat acaggcaata
     9901 cataagccta aaaatgttaa attcataaca aattctcctt taaaataaaa tttacttact
     9961 tagtgtagtc ctaaaattta aatttgtcaa cttttaaccg ctattggaag cgtttaataa
    10021 aaattatttc cattttgcta aatttcaagt tcaagttagc catccagaag tcttctctgg
    10081 gtgacttgta gtccaagcat tttttaggat agttgttaat ccacttttcg atgaatgcga
    10141 cttctttggg agtcattttc ttggttccct taggtaacta tctacgaatg agcctgttgt
    10201 gattctcatt agttcccctt tcccaagagg cataggggtg cgcataatag atgtgctcct
    10261 cagaaaatat atcaaacaag cgattgaatt ccgttccatt atctgccgtg atggaaagaa
    10321 tcttgtgttg ttttaagatg agttttagag cctgattgac cacctcagca cttttatttg
    10381 gaatcaatcg gatgatctga tgtctactct ttcgatccgt caagacaatc aagcagtagt
    10441 ttttcgatct cgtaagtaga accgtatcaa tctcataatg cccattctcc aagcgaagat
    10501 tgatagcttc aggccgctgt tcgatggatt gaccagcagg tttaaagttg gtgctagcct
    10561 gtttcttaag cgcttttcct tttctagggt aaagcaaatc ctgcttgctt aaccccaatt
    10621 ttccatgatg aatccaatag taaatggttg aaattcccac gttaacccct ttagccataa
    10681 ccatcatttc aggcgaaaat ttttggttat gatagtggag aatcttttcc tttagttcct
    10741 tggtcaagct tgatttcttg accgagcgct tgcgattgtt ttcataagac tgttgagcgt
    10801 agtcggcaga ataaacctct ttgaagcgcc cttttccaag acattgtcgg actgtcccac
    10861 gcttgatttc agtgtgatag tttgaggagc ttttccaagt agagaggcaa tttctctatt
    10921 tgattttcct tcttttttcc atctttcgat taagcgacgg ctatcgattg tcaaatgttt
    10981 ggcttttgta gtataattgt cttgcatctc tgtgcctttc ttgtgtttgt ggttgaacaa
    11041 caagtataac acagaggtgc tttcttatgc ctacaagagc tttcattatt tccattttct
    11101 tttggatttc actctattct gaaaaacttg tgtatattta ctgaagctag caagtcttac
    11161 ctgtaaattt aatgaaagca acacaaaatc cgagagggga atctcggatt aatagataga
    11221 gagtttttag tttaaataaa ttgtttaaaa tatcaacaac atcacttctt ttcttaacct
    11281 gataagtctt gattcctaat tttggggcta cgattatatt gtcctcaata tcgtctagaa
    11341 agacacaatt tctaggttat aactggtatt tatcgatagt tactcatata catcagtcca
    11401 cctccatact tatgtgcgag cctctctttg tattatacct ccatactcac cttacagatt
    11461 cttttggtaa taatatcttt gcctaatgta gagacagtct tgcaaagaaa aaacttcctt
    11521 gtagccatgt ttctgataaa agtccggtgc ctggaactgg taagtattga caaaggcaaa
    11581 acaacaattt cgattcttag cttcactttc tgcctgttgc aatagttttg aaccgattcc
    11641 ttgccctcgc agttcctctt ttacaaacaa atactcgatt tctagccaat ttccaaaagt
    11701 ctctgctatc aaacctgcca ggagattgcc cttttcatct tcgacataaa gattaagtgg
    11761 ctcactttca gcctcttctc tttttgaacg gttataaaca cgaatcagat tccctatttc
    11821 ttgcgattta tgtgattcct tattttccaa tctaaagtat agtgaaatga aataaaacat
    11881 gcgcaaatcg attaaggaat ttaatctaat ttctaacaat gtcttagaaa tcaaagtgta
    11941 ctattttaac ttcaatgcac tatacatcta atactcaata aaaatcaaag agcaaactag
    12001 gaaactagcc gcaggttgct caaaacactg ttttgaggtt gtagatagaa ctgacgaagt
    12061 cagctcaaaa catagttttg aggttgtaga tgaaactgac gaagtcggct caaaacatgg
    12121 ttttgaggtt gtagatgaaa ctgacgaagt cagctcaaaa catagttttg aggttgtaga
    12181 tgaaactgac gaagttagct caaaacactg ttttgaggtt gtagatgaaa ctgacaaagt
    12241 cagtaaccat acatacggca aggcgacgct gacgtggttt gaagagattt tcgaagagta
    12301 gcaaaatgga aaaaggagtg agtgaagcac atcgcctccc cactcctttt tctgttttta
    12361 ggctgttttt tcaaccttca agatttttac atcatagcta ccaacaggcg tttcaatggt
    12421 tgctgtatca cctgttttct tgccaatcaa ggcctgccca attgggcttt catttgaaac
    12481 cttacctaca aaggcatctg caccagctga acctacgata atataaactt cttcttcgtc
    12541 ctcaccaatt tcttggatgg tgactgtttt accaatcgct acttcgtcct gggcaactgc
    12601 gtcgctattg acgatttcag catagcggat ttttgtttct aagctagaga tttgtccttc
    12661 gacaaaggct tgttcatcct tagctgcttc gtactcactg ttttctgaaa ggtcaccgta
    12721 tgaacgggca atcttaatgc gttctaccac ttctggtcga cgaaccaatt tcaattcttc
    12781 taattctttt tcaagttttt ccttttcctc aagggtcata ggatatgttt tttctgccat
    12841 ttttctcaac tttcttctga taatattttc taaagaaaat tatgtgaagt atcacataat
    12901 tttagtttgt ttagtttaat ttgctgttga catgttcagc gacattgcgg tcgtggtctt
    12961 cttgattgtt agcatagtag accttgcctt ctgtgacatc tgctacaaag tagaggttat
    13021 cgctcttagt ttgattgatg cttgactcaa tcgcatccag acttggacta tcgactggac
    13081 caggcatgag acctacattt ttataaacat tataaggtga atcaatgttg gtatcaatcg
    13141 caacatcctc agctagactg atattttgcc ccagttttcc ttgggcatac aagattgcaa
    13201 tattactttg aagtggcata tcacgattca aacgattgta gaatacacct gcaatgagct
    13261 tacgatcttc tgtcttggca ccttcttttt cgaccaagga agcaatggtc aacaactcat
    13321 tgacagtcaa gtttttagat ttgatagtac tatagtaagg agataggttc ttatccatag
    13381 cagctaacat ctcatcaatc aagctctcaa tagttgtgct ttccttgata gagtatgtag
    13441 ctgggaaaag gtatccttcc aaacgataac gcgcaccgct gtcttttaca ggcaaacttt
    13501 ccagtaaagt aggatatttc gctactgctt gactgataaa cgtctcatct tgaactttag
    13561 ccaagaaagc ctccgctgtc aaagactctt tgaagtcacc ttgcaattga cccacagctt
    13621 gagcaatctg atccaaggta taaccttctg gaattgtcaa agtcgcaagt acaggttctt
    13681 gcggttcatc tgttccacct ttttgcaact ctttgagtaa gtcttctgta ctcatactct
    13741 tttgcaaatt gtagtaacct gctttcaagt cggtataatt tttatacttg gcataaaaac
    13801 taaaaatcag accatgcttt accaaaccag ctttttcaag cgtcgtaccg atttcttgaa
    13861 cgtttgaacc ttctggaatt ccaaccgtca catatttctt agaattagca tcgataggta
    13921 ataaggaatc tagcacgtac tggtaaccaa agtaaccacc tgctgagagc aaggcaagga
    13981 atacaaggaa tgaaatcaca aaagctttca catgtgactt cttctctttc ttaggcttaa
    14041 cgggttttgc tccttctcta cggctacgac gtgatgtatc tctgatgata tccactgttt
    14101 ctgtagcagg cgtaggtgtt tctggacctg cttgttctgc ttttttctct gccgtcgtat
    14161 aggaaacagc tacccttgtt ggggtttcat tgtattctct ttcaagtttc ttaggtctaa
    14221 caggacctgg acctggtctt gatccacttt cttccgctgg agaagaaggt acatcttgac
    14281 ttggatgact tggaacacca ggagtttctc tttgaatctc atctgctgga gaagctggta
    14341 caccttgact tgggtgagta ggcacggtag gagcttttct cataatctcc tctaccgttg
    14401 acaaggaatc agccatgagt tcttcagttg aaggttcatt tgcaggagtg cgaactactg
    14461 cctcatcttc tttcagaact tcatcatagc cttttacttt ttctaaatct ctcagaatct
    14521 gctctttaaa gcttaatttc tcttcttctc ttgacttttc actcaaaagt ttttcctcct
    14581 tgttgataat ccataatatt atatcttaaa agtccaaaaa aagcaatcta tgatactttt
    14641 cctaacttat tttttcattt cccagaccat atcataccat gtttcccctg caaaggttga
    14701 ctgggactgg ccttcgttga caaatccatg tttttcatag taggcgatga gatagtcatg
    14761 acaggttaga ttaatgccat ctctctcatc ttcaagagca acttctttca aggctgtcag
    14821 caatttctgg cccagtccga gtccctgtgc ctccttggca atagacagac aggtcacaga
    14881 gatataacca ccaggctcat gactatagtc ttttatttct tctgtaaaag actggtcttg
    14941 cagatggcgg tgcaggccaa ctggtccttc gatataaccc atgattcttc cttctttttc
    15001 agcaaccaga aaagaggtct gaatttctct caaatgtgct tcaaagacag aaggaggaat
    15061 ggcttcttcg accgaaaaat tttcaaattc aagttcaaca atccgatcca aatcttctaa
    15121 tcttgcttgt ctgattttca ttgttcctcc agataaaagg gattaaacca aatcatacta
    15181 tagccctggc tagttgcata gagcaaagtt gcttcttcat caacaaaacc gttcatttca
    15241 aaataggaaa gcagctcatc aggactctcc aaacgaatcc ctttgtaatc cagctcaact
    15301 gccacctctt tcaaggctgc aagaagaagt gttcccaggc cctgtctctg atggtcaaac
    15361 tcgatgacta aagaatgtac ttttagacat tgcggattgt ctgactgggg acttgataaa
    15421 atatagccta aaagttgatt ttcatcccta gctagaagaa aggtatccgc acacttacgg
    15481 atactttctt ctaaaatatg ggaaagttgc tgcttttcag ctggaaaaga cgaggtctga
    15541 agtgccccta tctcaggcaa atcagacttg cttgcctgaa tgatcttaat tggaatttcc
    15601 atgggaacat cctattgaac attgcttgtc aagttagaca agagacgctc aaatgagtat
    15661 tcataggttt ggatgtctcc tgctcccata aagacgtaaa cagcattgtc atggtctagg
    15721 agtggagaaa cattttcaac agtaatcact tggtgttttt tgttgatttt gttggctagg
    15781 tcttctacct taacgtcacc atgatctact tcacgagccg agccataaat ttgcgctaga
    15841 taaacagcat ctgcttggtt taaagcatgg gcaaagtcgt ccaacaaggc aatggttctt
    15901 gtaaaggtat gcggttgaaa gactgctaca atttccttgc ttgggtattt ctgacgagcc
    15961 gcatccaagg tcgcaataat ttctgttgga tggtgggcaa agtcatcgat aatcactgta
    16021 tcattgacaa ttttctcagt gaaacgacgt ttaacaccgg caaatgtttt caagtgctca
    16081 cgcaccaagt tcaaatcaaa tcctgctgtg taaagaagac caataacggc tgtcgcattc
    16141 atgatattgt gacgaccaaa ggttggaatg tggaattgcc ccaagttttg tccacggaaa
    16201 tgaacggtga aggttgaacc agttattgaa cgaagaagat cactagctac aaagtcattg
    16261 ccttcagctt caaaaccata ataataaatt ggtgcatcag acgtaatctt acgcaattca
    16321 gcatcttcac catagacaaa aagacccttg gtgatttgtt tggcatagtc gttaaaggca
    16381 ttaaaaacat cctcgagact tgtgaaataa tctggatggt caaagtcaat gttggtgata
    16441 atagagtatt ctgggtggta aggcatgaag tgacgctcat attcgtcaga ttcaaagaca
    16501 aaatatttgg cattggccga accacgacct gtcccatctc caatcaagaa gctggtatct
    16561 gtaatgtgag acaagacatg agacaacata cctgtcgttg aagtttttcc atgtgctcct
    16621 gctactccca tgctaacaaa gtcacgcata aagctaccta gaaactcatg gtaacgtttg
    16681 tagctgatac cattttggtc cgcataggca atttcgacgt tgttatctgg acgaaaggca
    16741 tttccagcga taatttccat atcaccgtct agattttttt catcaaaagg aagaatggta
    16801 attcctgcct gctcaagacc gcgttgggta aagtagtact tttcaacatc tgatccctga
    16861 accttgtgcc ccatctggtg caacatcaag gccaaggcac tcatccctga tcccttaatt
    16921 ccgataaaat gatatgtctt tgacatgttt tctcccctat tctgtcattc tggtcagatt
    16981 caactcttgg gcaacccgac gttcttgttc tgtttgttta ctttttttat tgtagatttg
    17041 gctcttcttt agaaaatcat aattgttttt ctttggagca ggtgctgaca cttcttcatt
    17101 cttggtaggg atagaatgaa cttcttccgc caagatataa tgagactggg tcaatttttg
    17161 gctatatttg acaaattcac caggattttc cttttggaaa ggagctgtcg gttgattgcc
    17221 ctgtctaact agactgggct gagaatgacg tctcgcaagg ctgaaatcct gagttaggta
    17281 gttagcagag cgtttctttt tcaagtccgc acgcgcttct tcacgcgcca cctccgcata
    17341 gctctttcct tcttttttaa cccctaaagg agccttttta ggtttttcga cttgcttttc
    17401 aatcggtttt actggttttt cttcagcaat aggagcccat tctaaataat ttttatctcg
    17461 atactcaccc ttgatattac tgatcagatc agactcatca tagagattca tgactggcat
    17521 ttcagtcaac atgacctcgt catctgacac caatggaaat cgttcttgtt tcattttcta
    17581 tttcctttca acacttcatt atagcgtatt gtcttgattt ttcaagtgct ggcttcagaa
    17641 attcccaaaa tttctctaat ttctgctagg gtcagactac cacgtgactc tgtgccgtcc
    17701 aatacttgtg acaccagatg tttcttttgt tcttggagtt cctgaatttt ttcttcaatg
    17761 gttcccttgg tcaccaagcg atagacctca accgtttctt cctgacccat ccgatgggca
    17821 cggccaatgg cttgcgcttc caccgcagga ttccaccaaa ggtcaaccaa gatcactgta
    17881 tctgcacctg tcaggttcag accgacccca ccagccttga gggaaatcag aaaggcatct
    17941 ctttctcctt ggttaaaggc cttggtcatg tcttgtcttt ccttggctgg ggttgaaccc
    18001 gtaattttaa aggaagtcag gcccaagtct ggcagttctt gttcaatttt ttccaacatt
    18061 cccttgaact gagagaaaat caagacacgg tgtccgccgt ctgccacctg taccagtagg
    18121 tctcggagac tatctagttt gccgctggct ccctgataat cttccataaa cagggcagga
    18181 gtgtcacata tttgacgcaa gcgcatcaaa ccagataaaa tttccacacg acttcgctga
    18241 aattcctgtt ctgacacttg agccagatgg tctcgcatct gttgtaactg ggcaaggtaa
    18301 atagcctttt gctggtcttc cagttcattt ttataaacca cctcaatcaa gtctggcaat
    18361 tcagtcagaa cttcttcttt cttgcgtcgc atcacgaaag gcttgataaa ctgagccact
    18421 cgctctgctg gcaatttcat aaattctttc ttgcttggca aaagtccagg catgacgatt
    18481 tggaaaatag accacaactc acccagatgg ttttcaatcg gagttcctga caaggcaaag
    18541 accgacggca ccacaaattg tctcaaggtc tgggcaatct tggtctgggc atttttcatg
    18601 acctgagcct catctaagaa aaggaagtca aaggccatcc cttgataaaa ctcactgtcc
    18661 tgacggaagg tggcatagct agtcacatag atttgatggc tctcggcaag aatctcctca
    18721 cgacttgctt tcaaaccatg aacaacagtc acatccaact gtggagcaaa tttctgaaac
    18781 tcatctgccc agttgtaaat caaacccgac ggagcgagaa tcaaaacccg actttctttt
    18841 gtcacttgac tagtcaaaaa agcaatggtc tgaagggttt tcccaagtcc catatcatca
    18901 gccaaaatcc caccaaaacc ataatgatgg agcatctgca accagccaat tcccttttcc
    18961 tgataatctc gcaagtcagc cttgacctga gttgcttgca aaggaaagtc ctctggatgc
    19021 gtcaaatcct gggccagatt ctggaattct tgtgaaaaag aaacacggtc tcgcccttca
    19081 aagagatgag ctaaactgta ggccaaggat ttccgaacct gcaaggtccc atcttttaat
    19141 tcaaattgcc ccagttcctg tagattttgg cgaattttct tggtttcttc atcgaaaaag
    19201 taaacttgat tagacgaatc aatataaaaa tcctgattgg caaccaaggc ctgcatggct
    19261 tggtcgattt cctcctggac aatattttga aaatcaaact ggatttccaa gagacctccc
    19321 ttggaggcaa tctgcacctg aggactcgct aggctataaa gctcttctag tttatctgat
    19381 aggtcaacat gcccgagttt ttcaaagact ggaatgatat catgaaaaaa atgatagaca
    19441 gactccgctt ttaaggcctg acgccaagat tgaaaatcgg cctcaaagcc cgcagccaaa
    19501 cagacttgga aaattctttc ttctaagtct gcgtcacttg aaaagggtaa ttcttctagc
    19561 tcttgtcggc tagatacctg tctatttcca taatcaaact gaatttctaa acgaatccga
    19621 ttatcttctt ccctgtcaaa gtaaaaagag ggcgcaaaag ttttgatttg tagacgttct
    19681 ggagctgaaa cggtgcccat ctggataaaa agagtcagac aggaggccaa tttgtctcga
    19741 tcactgctat caaattgcag gtatttcttt ccttgttgac ccacaggtaa cgctttaatt
    19801 tccttgagaa gacgcatctg ctggtctgtt aaaaaataaa cctgaccttt atggaaaagt
    19861 actgctccct gataaaagac attgacccta ggactctcac tgatttccat ttcaaaataa
    19921 tccgagtatt ctgttactgt aaaggcaaat agattggcat cagcatgcat atcctgaaaa
    19981 agcagggttt ggtagctatc cacttgatgg tcaaattgaa aatggggcaa ggccatcagt
    20041 aaattcacac cctgctcaaa aaaggtcaga gggaaaaaga ggtgccgacc ttggttttgg
    20101 aaaaagaggt ctggaaccag cccttcctcc gttagtccgt gcaagaaagt caaaagttct
    20161 tggctggcat catcaaaggc ttcccaagaa agagactcct cataaatctt gccaatcata
    20221 tacgactttc tctgctcgac aatccttaaa aaaagtggaa tatcccgaat gacatagtat
    20281 ttttggctat tgatttggcc gattctcaga gtccacaaga tatgattggt tcctgcttcc
    20341 acctgaccca cagctgataa ctcataggcg cattctgatt ttggagataa aattcgatcc
    20401 aaaaacttgc cacccaaggt caccttggtt tcaacagcct ctttttcttc atgaccttct
    20461 tccagactcc acaagatttc ctgaccacgc tcatcatttt tcagaaaatg ctctagcgct
    20521 gccaaatgca cacagtagcc cctcttttga aaaaaatcac aggcacaaaa aaccaaatca
    20581 tcctctaaac tatagcgcag ttcttcttct gcaacgcgag cgtagagccg attgttcttt
    20641 tccttgatga tatcaacctt accagtttca taaagggcaa caccttcgat acgaattttc
    20701 cccggaatca atttagccat attttcacct ttaccttatc tttttattat accatatttt
    20761 cgcctatgaa aatagccttc taggaagact tttctcctag aaggctggat ttttaacgtt
    20821 tggcaaaagt agccacaatc cgctgacaga cttcttgcaa cagagatttg ggcatagcta
    20881 tattgatgcg ggcatggaga cttccttcct ctccaaaatc caaaccacgg ttgaggataa
    20941 ccttggcttc atttctcaac aactcttgca atgtttcatc agtcaggtca taagctgaaa
    21001 agtcaagcca aatcaagtag gtaccttgcg gtttcatgac cttgatttta gtctcttttc
    21061 caaatagatc catcacataa ttgatgtggt cttcaaagac ttgcttgagt tcctctagcc
    21121 aatctttacc gtatcgatag gcagcttctg tcgccaaata acccaagcct gaaatttcat
    21181 gctgattatt ggccaacagg cgtttctgga aagccagtct caacttagga ttttcaatga
    21241 ctgcatagga attttttgtt ccagcaatat taaatgtttt agtggcactg ctcaagacga
    21301 tagcaaaatt tttgaaggca ggattgatgg tattgaaaga ctggtgtttg tgaccaaaga
    21361 gggtcaaatc ttggtgaatc tcatccgaaa ctaacaaaac accgtgtttt tggcagagtt
    21421 ggccaatctt ctccaacact tctttttccc aaacacgtcc accaggattg tgagggttgc
    21481 aaagaacata gagtttaacc tcctcttcca ccaaatcctt ttcaagttgg tcaaagtcaa
    21541 tctcaaacag actatccttt tccactaagg aattagtaat caatctacga ttattcaact
    21601 tgacactgcg agcaaagggt gggtagacag gcgtgttaat taaaaccgcc tcgccttctt
    21661 ttgtaaaggt ttgaatagct gttgagatgg ctggtaccac accctcgata aagacaagag
    21721 cctctttgtc aaagttgtaa ccgtattgtg tagcttccca cttttgaact tccttaatta
    21781 agtcttcact ggcataggta taaccataaa ccagttggtc tgcgtaagtt tgcacggctt
    21841 ggcggatttc aggcaagacc acaaagtcca tatccgctat ccaagctggt agaacttcac
    21901 tatccgtttc tgtttctttc catttatagg tatggtgccc taaacggttg ggcaggcttg
    21961 taaaatcata ttttcccatc tttgtcttat ccttctatgg cttggcgcaa atctgcaatc
    22021 aaatctccag catcctcaat cccaatagac aaacgcaaga ggtcatctgt caaaccataa
    22081 gaatggcgta cctctgctgg aatatcagca tgagtttgag tcgttggata agtaataaga
    22141 ctttccactc cacccaaact ttccgcaaaa gagaagacct tgagactgtt caaaatatga
    22201 ggaatgcgtg tttcatcggc tactttaaag gaaatcatgc ctccacgacc agtgtagaga
    22261 acttccttaa ctgctggaga atccttcaaa aaggcaacca cttcttgggc gttagctgtt
    22321 gagcgctcca tacgaagaga caaggtcttg agaccacgaa gcaactggta gctgtcaaat
    22381 ggagacaaga ctgcccctgt tgtattaaga ttgtaaaaaa gcttctcgta tagttctaaa
    22441 ctattggtca caaccactcc agccaagaca tcattgtggc ctgctagata cttggttgct
    22501 gaatggagaa cgatatctgc tccatcttca atcggacgtt ggtagatagg gctatagaag
    22561 gtattgtcca ccaccacttt ggcaccctta gcatgagcca attttgctag tttttcgata
    22621 tcaaattcca acatcaaggg attggttggg gtttcgatat agagaacatc cacatccttt
    22681 tctaactcgg caatcaactc ttcttctgta ttggcatagg taaaatggaa atgaccttcc
    22741 tgctccactt ggttaaacca gcgaaaagaa ccaccgtaaa gatcacgcac tgccaagacc
    22801 ttacttccta ctggaaagac gctaaaggcc agtacaatag ctgacatccc tgagctagtc
    22861 gctagggcat agtctgctga ctcaatagcc gccaagactt cctcagcctt actacgagtt
    22921 ggatttttag tgcgcgtata gtcaaaccca gtagatcgac caaactctgg atgctgatag
    22981 gtcgttgaaa aatgaagtgg tgtcaccaaa gcacctgttg cctcatcaga cttgatccct
    23041 gcttgtgcta aaattgtgtt aatgtgtaat tccttgctca tacaattcct ccaaatctat
    23101 agtaactatt gtaccactta ttttgtatcc ttcgttttct tgttttcaag agctagttat
    23161 agtttcaaac tatataaaaa gggagttttt cctgctccct ttaatagact ataaaatggt
    23221 gaatctcaaa agacaccttc actctatcat ttgctcctgc acaaaacgag cataacgctc
    23281 atgattttcc agtagttcct tatgagttcc tgagccagtg attttcccct cctctaagaa
    23341 gaaaatacaa tccacatctt ttaccgttga caaacgatgc gctataatca caaccgtctt
    23401 ctcctttagt acagaataga ggctactgat aatcgcatac tcagaatccg catcaagatt
    23461 agcagtggct tcatcaaata taagaatttc agcatctttt aagtaggctc tagctatttg
    23521 aagtctttgg cttcgccccc ctgacaagag tcgtccgcgt tcaccaactt cagtatctag
    23581 tccctctttc atggagcgaa tctcatcacc tagtgatact aagtctagca ctttcatcaa
    23641 ttcatcatca gttactaagc gattcaaacc gagacaaaga ttgtcacgaa tactgccaga
    23701 taagactgca ttattttgtg aaacccaagc gattttactt ctccattctt ttaagttaaa
    23761 atcatatata cttgattgct ccattagaat atctcctgaa agcggtttat aaaaccgctc
    23821 taacaaacgc acaatcgttg attttcctga tccagatggt ccaacaaaag caattttttg
    23881 ccccttgaaa attgaacaag taatatcctt taagacaggt cgattttcat cataaccaaa
    23941 atagacatgg ttaaaattca accctcgtcc tgataccgat tttcctccct caaatttttc
    24001 tttaggaact gcaagcaagt tctccagtgc aactgaagat cccttgctcc tagaataaac
    24061 agttacaaaa ttagctatat tactaatagg attaagtaat tgaaagaggt aaatcaaaaa
    24121 cgaaaccaag gttcccacag atatatatcc tgcgctgacc cgataacccc cataggttag
    24181 catcacagct atagtcgcaa agataaataa gagagcaaac ggggtctcaa aagaagtaac
    24241 cctatctgat ttcagtgaat tgttttgtac cctttcaata caattatcca aaacatcctg
    24301 tacacttttc tctgcttggt tagtcttaat taattcatgt tcttgaatct tttcagtcaa
    24361 ttgccctgtt aaatttcctc ctgtaaacga cgactatact tttcactgat attggaaagg
    24421 ggcaagataa taaacatcat acaaggaaga gtgatgaata aaagtagaga aagattccaa
    24481 tcaagactaa ataagactac aatggaacca agtaccataa ctaaactcag aataatattt
    24541 gggaaagtcg taattaaaaa ctcacgaatg acactcgtgt cattgacaat ggcagaagtc
    24601 aactccccac tttggctctt atcaaagaag gatttctcta cataaatcaa cccctctatc
    24661 acttttttcc tgatttttgc tatctttttt tcacccgatt gactaaacag atagtaacca
    24721 atagaagaaa acaaggcttg accaataaaa atcaaaaacg attgaaatac tttggagcct
    24781 atattttcaa tagaactccc atctattaaa tcctttaaga taaggggaag caacaaagca
    24841 agtagactag acagaacaag taagaaactc cccataatca ccttagtatc tactcttaat
    24901 aattttaatt tcataaatac tccttataat atttcaacgg ataaagtcgg gaataactca
    24961 atttgaggat aaaatctaat aaatcttcct ataacaaaac gcataacatc taggatttta
    25021 tatacctgat attatgcgtt tttaagcaca aagacttctt acacaaactt atctacaatt
    25081 agattttatt tgacatgttt tgccaattct tcttgggctt ttttattgga ttcttctttt
    25141 tctttcaacc atttttctct ggcttttgca tattcgtctg ttgtgacaat cttatcttgt
    25201 actttgaggt atttatatga ttcaacccct tttgtaccgg ttaaaccata ggcagcagca
    25261 aatggtacgg ttcttctcaa tgatggtgtt cccccacgcg aaacacttgg aagaactaaa
    25321 gaactatcaa tcaaccaagc ttgaatatca gcatatttct cataacgttt ggccggatct
    25381 tgctctttat tagcttcttc caacatttga gtatagacat ccagtccaac tgccttagcc
    25441 ttgtcattgg cctcaccagg ctctagtcca agattttgca gaaatcctcc actattagta
    25501 ttaaaaatat cgagataggt tgacgggtct tgataatcag gtccccaacc gccatgatat
    25561 aaatcataat ctttctgagc agctgtttga gcaaagtagc ctgaactgtc aaactcatct
    25621 gatgttaatt gctgaatgtc aatcactaca ttatcagaac ctaaaacaga ttcaattgat
    25681 tgtttgatag aactaactcc ttgtatgcct actttatctg ttacttccac agtcttatcc
    25741 aagtggattg ggaattgaac accctttgct tcgagttctt tcttagcttc cgcaaactta
    25801 gccttggctt tctcaggatt gtagtaaggg tcttgaccat ccgcaaagtt gataccttgc
    25861 cattccttac catagttgac catcttagag gctacaactt caccaaagtc ttttcccttg
    25921 atactgacaa agtttggagg aaccactagg ttacgcaaaa tctttgttgc accttctttc
    25981 ccttcagact gagccccata agatgttctg tcaaaagcaa aattgatagc ctgacggaag
    26041 tttttattga gaactgcttc ctgagtcgat ttcttttcaa tgtcacttgt tttagaagta
    26101 taattgtaag acttcctatc taggttaaaa ttaaagaaat atgaagttga attttgcata
    26161 ctatagatga tattgttttt gtatttttct ttaatccctt catagctgga gctgttagga
    26221 aaaagacgag ccgtagtata agcaccagct gtaaaattac gttccagtga ttcttggtcg
    26281 ctaccatcat agtaggtcaa tttcacatcg tctacaaaga cattcttagc atcccagtaa
    26341 ttagggtttt tcttatattc aatagcagat tttgagacaa gtgctttcat caagaaaggt
    26401 ccattgtaca aaatactaga tggatccgcc ttcccaaaat catccccttt tgatttcagg
    26461 aaatctgcat taacaggaaa aagtatcgtt gcaagtgttt ttgaattcca gtaaagttct
    26521 ggtttaacca aagtatattg aaccgtttgg tcatcaagtg ccttgacacc gacagttgaa
    26581 aagtcgcttg ttttaccagt gatatagtca tccaaaccag caacagagtc ctgcactaga
    26641 tacaaggctt ctgatttttt atcagctgca tattgcaaac ctgtcacaaa atcctgggca
    26701 gttacaggcg catattcttc tccctcagaa gtaaaccact tggcatcctt acgaagtttg
    26761 taggtatagg tcaaaccgtc ctgagaaaca gtccaatcct ctgctaatga tggaataata
    26821 ttcccatatt ggtcattttc taataacccg tctaccaaat ttgcaacaat atcggatgtt
    26881 gctgcgcggt tttctgctag atagttcaag ctagatggat cacttgaata aacatagttg
    26941 taggtttttg acgccgtgct agaatttcca cacgcgctca ataaaactcc tgtacccagg
    27001 acaagacctg ccaaggttag atatttgctc ttagactttt tcatttccgg aacctcctgc
    27061 tttaaatatt tgttttatta tacaactctt ggtttgttta gtcaattgtt ttttccaaaa
    27121 aatcaaaaaa acctcatttt ctatttttga aagataattt tagctatttt tacgctaaaa
    27181 aaggaataag tttcaagttc tcctaaaaga ttgttcaact tattcctttt atttataaat
    27241 gctaccttac gaaagcttaa attttgctcg caggcgatct gcttgggctt ttcctattac
    27301 cttatctaat aaataggtac gcagactcat aaccatataa agtccacccc ccatggcacc
    27361 gacaagagct acataaaaga agctccacaa acgtccactt ggttggaaga aaaatcctaa
    27421 cagccactgg atggttccta ttaacagaaa catgactagg gtcagcaaac tgattaaaat
    27481 ggttcgcttc aaaatcacct tgcgcttgac accagttact ttacaaatat cccgatacat
    27541 caagacgtta ggaatgatga gagcaatggt tgttgaaatc aaaggaccat aactgtggaa
    27601 gagggcgatg gtaggtagtt gcaagactag cttggcaata gaaccataga taaaatagag
    27661 aacggccttg cggttgcgga acatggcctg aagcattgga gacaagacca tgtacaagcc
    27721 taaaataata gactgcaaaa ctgcaaagac aaataagccc agagccaaac tatctggctt
    27781 accatagaag accgtataaa gaggttctcc taccataacc actccaaccg ttgctggtag
    27841 caagaacata aagagtaggg tgagactgtc ctgaacgaga cgagaagctg ctttcaagtc
    27901 ccccttgaca tagttttccg tcaaaagtgg caaaccaaca ctcccaatcg aaacccctac
    27961 agaaatcaaa atcatcgtga ttttattagg attggctgag aaataagaaa acatgacaac
    28021 caagtcctca ttgctgtagt tggtaaacca gctcatacta ttgataaagg tcagctgatc
    28081 caaaatctgg aagagctgga tggcagaccc tgtcaggata aaaggaatgg cttccttaat
    28141 ggtatcaacc aagagacgct tactgttaat cttatctcct gtttcaaaga ttcttttgag
    28201 tgaaccttct tgggcaagga aataaatcaa gactgcaaaa ctggctacca taccgacaaa
    28261 ggcagcaaag gttgattggg taacggctgc tagataatct cctgaaccga gcttcataat
    28321 gataaaggtt gctaggagca tccagataac acgaatgacc tgctcagcaa tttggctcat
    28381 ggcatagggt ttgaggttat tcatcccttg gaaaaatcct cggataacac tcatagacgg
    28441 gaaaatcaag actccccaag ccaagctttg catgattggg atcaagtctt tgcccacgcc
    28501 agacaagtct gctagccaag gagcaaagac atacaagact aaagcaaaaa ccaggcctag
    28561 tcctgtcata aagcctaaga agctccgaat cagggcaaag ctatgctctt cttctcgcat
    28621 ggtattatac ttggcaactt gcttggccac cgcaactgga atccccgctg ttgaaaccaa
    28681 caagaaccaa gcatagatat tgtaacccat ggtaaagaga ccatttgcct tagctgcata
    28741 agcccccatc cagatgtacc aagggataat gtaaacagcc ccgagtaggc gactgataaa
    28801 gttactagcc gttagccaag cagtcccccg taacatctgg gcctgctggt gattgttttc
    28861 gtgcgacata gattcctcat tcaattttga taactaggaa aaactcctta tcttccatta
    28921 taaacttttc cttgttactt gtaaatttac gactttcggt ttacaataga aagtatgatt
    28981 aagattgaaa ccgtattaga tattttaaag aaagatggcc tttttcgcga aattattgac
    29041 caaggtcatt accactacaa ctacagcaaa gttatttttg atagcatcag ctacgacagc
    29101 cgaaaagtaa cagaagacac tctttttttc gcaaaaggcg ctgcctttaa aaaagaatac
    29161 cttctttctg ctataacaca aggattagct tggtatgtag ctgaaaagga ctacgaagtc
    29221 ggtatccctg tcatcattgt gaacgatata aagaaagcca tgagtttgat tgccatggag
    29281 ttctatggta atccacagga aaaactcaaa ctccttgcct ttactggtac taagggtaag
    29341 acaacagcag cctatttcgc ctataacatc ttatctcaag ggcatagacc tgctatgttg
    29401 tcgaccatga acacaactct tgatggcgag actttcttta agtcagcgtt gacaacccct
    29461 gagagtattg acctctttga catgatgaat caggctgtgc aaaatgaccg tacccacctc
    29521 atcatggaag tctccagtca agcctatctg gtcaaacgtg tctatggtct aacctttgat
    29581 gtgggagttt tcctaaatat cagcccagac catatcggcc cgattgaaca ccctagcttt
    29641 gaagactatt tctaccacaa gcgtctcttg atggaaaaga gccgagcagt catcattaac
    29701 agtgacatgg accacttctc agtcttgaaa gaacaggttg aagatcaaga ccatgatttc
    29761 tatggtagcc aatttgataa ccaaatcgag aattccaaag cctttagctt ttcagctacg
    29821 ggtaaactcg ctggagatta tgatattcaa ctcattggca acttcaacca agaaaatgca
    29881 gttgctgctg gacttgcttg tctccgtctc ggagcaagtc ttgaggacat caaaaaaggc
    29941 atcgctgcaa cccgcgttcc tggtcgtatg gaagtcctca ctcagaaaaa tggagccaag
    30001 gtcttcatcg actatgccca caatggggat agtctgaaaa aactcatcaa tgtggttgaa
    30061 actcatcaaa ccggaaagat tgctctggtt ctgggatcaa caggaaacaa gggagaaagt
    30121 cgtcgtaagg actttggcct cctcctcaat caacaccctg agattcaagt ctttctgact
    30181 gctgatgacc ctaactatga agacccaatg gccattgcag atgaaattag tagctacatc
    30241 aatcatcctg ttgaaaagat tgcggatcgc caagaagcca tcaaggcggc aatggctatc
    30301 acaaatcacg aattagatgc agttattatt gcgggtaagg gagccgattg ttaccaaatc
    30361 atccagggca agaaagaatc ctacccagga gatacagccg tcgcagaaaa ttatttataa
    30421 gaatagaaaa aatcaaggga aaatgaaacc cttgattttt tcctattctt tagtaaaaac
    30481 gtttttcaaa ccttcaacgg caccttctac agcacctttt gcgtcttcta caacgtcttt
    30541 tgccttagca actgtttttt caacagctcc ttcaagttct gtcttgctgt ctccagtaac
    30601 tttaccaaaa ccttctttga cagcgcctgt tgcttgttcc aatttgtttt caagtgacat
    30661 atgattgtct cctttagttt attccgatat gtgttaccgg ttacatatag tttataccga
    30721 atactaagga aagtcaaaca atgtactcag aaacaagaaa tcacgctaag tagaaagtta
    30781 acctctcctt atcaaaatcg atagccaact catactttcc atcttcattt tgcacaatat
    30841 agcctaagac aacctaactg tccacaaaga tatcacggca tttctgcata agctggtctt
    30901 ttttgagaaa tttcaacaaa aaagccgtca tatacttgag ggcatactca ggattaacat
    30961 ctcctaaaat gtcatagagc tcctgctgtt tttctgtcaa aggatactgc tgtttgacct
    31021 tgtaaaaata attggacagg gtcattttaa tgcgcgcaaa gtccgtcttt tcaactaaaa
    31081 tagctgcatt gatttgattg cgcaattccg tctcaaaact ctgctccaac aaggcttgat
    31141 agaccggact agcttctctg acaaaaatct cttgatccag ttcaagacta tcaagtgatt
    31201 caagcgtagg aaaattgagg taatagcgct tattttcacg tagaatcaag cctgccttta
    31261 tatactcttc catgagttta tcaactgcaa catctggaaa ttgagcctta atttcccgta
    31321 gaatcacatc ctcatactga tccagatagc ggatcaattc tccaaaaatg gctgtctcgt
    31381 caaacgagat ggattaaaaa tctgaatcat gaagattcct ttctttacat tgtaacagag
    31441 gcgatgctgc caactgacta ggattggtac cagcttggtt cagaggaaca ggcagaccta
    31501 gttctatgta atactccctc caaaaatcac taatctcctg ctccccatct ccaaatttca
    31561 taggaatagt ttcaaaaata ggtaggattt ctgctatata ctgggagcag taaaaaccag
    31621 ctgcatctgg atagaaagaa gcattgtagg gtgctccaag atgtttgcaa gctctttcct
    31681 tcaccgactg gatatccatt tctgggtaaa catagaggtc gtataaatga ttggactcaa
    31741 agaagtctgc cggttcttga cagacaacac cagcctgtcc actagcatga taaatcatcc
    31801 catccaaata aatggcaaca tggctatagt tacctgtgga agtctggatg gcctgtccca
    31861 tgtctgaccc atctctcaca aaaatcaaat cgccattttc taacatactt cactcctaga
    31921 aaaaaggagc cgaaactcct tgcgatataa ggcaaactag actttccgat tttgagctga
    31981 cacccaaaat cttacgcatt aaagctttca gtcaattgag gtaccacttg tttcttacgt
    32041 gaaacggcac cagcaaggaa ggcatgattg ttttcaagtt tgaagttgaa agccgcttcg
    32101 accttgtcca tattggcacc aagagccaag atttctgagt ttgagttgac gatatctgta
    32161 atcatcaaga caaagtcaga gtagccgttt gattcgttgg cagcttgcat tgcagcttca
    32221 atttctgctt ggcgttccaa aacttcagcg atgtcaactg tgttcacttg ggcaacacgg
    32281 acattatttc cgttgagttc aaaagtctta gcatcgatat caatcaattc ttcagcagat
    32341 ttgctagcca agttggtacc agctttcaac attgccaaac catattcttc caagttcaca
    32401 ccagccaatt cagccaattc aggagcaatg attttatctg ttgggtgtgt tgttggtgat
    32461 ttcaaaagaa gggtatctga aatcaaacct gaaagcatca aacctgcaat ctctttaggc
    32521 acagctacac catgttcttt gaacatacgg taaacgattg aagacgctga tccaactggc
    32581 tccaaacgca tgtaaagtgg gcttgcagtt tcaaagttag ccacacggtg gtggtctaca
    32641 acaccgtaaa cttctacttc agcgatatct gatacagatt gttggaattc attgtggtca
    32701 gtcaagataa cttgctctgc cccctctgct ttggcagaag tgataacacg tggtgcttcc
    32761 acaccaaaat agttcaagac aaaggctgtt tcttcatttg gagttccaag ggcaacagct
    32821 tccgtatcca aaccgtaagc ttcttttgca aggtaggcaa aagctacaga tgatccgatg
    32881 gcatctgagt ctggattttg gtgaccaaat actagaatct tggacatgat aatacctcat
    32941 tttgtttatt ttctttattg tagcattttt ttcgcttttt gacaaaggta agaggagtca
    33001 aaggactccc cacgattctt caaaataact gagtaaattt ctatcttgat tttcagataa
    33061 aaattcttcc ttctgtggcc tcttcttacg cttgagaaga gcttcagccg acatggcttc
    33121 ttccttactg gcaaaacctt gagcatagat aagtttgact ggcaagcgtg ctcgtgtata
    33181 tttggctccc ttcccactat tgtggatagc gaggcgtctt ctcatatcag tcgtatagcc
    33241 tatatagtag gatccatcac gacactccag aacgtacata taagccttat gatccataat
    33301 aaatctcttc gatttcgggc gtataagagc catcatcatt gtggacaatc aaaggaggta
    33361 agaccttaaa gccacttgtt gagccatcct tgatcgcctc aatcaaaagc atattggctt
    33421 ccttttctct ttttggataa acaaactgca ggcgcttagg ggctagatta tgtcgtttta
    33481 acgtatccaa aatatccaga agtcgatcag gacgatgaac catggccaaa cgcccattag
    33541 acttgagaat actctgggca ctacgacaga tttcttccaa attagtcgtg atttcgtgtc
    33601 gagccaagag ataatgttca ctctcgttca gattagaata aggattcacc ttgaaatagg
    33661 gtggattaca caaaatcata tccaccttac tcccctgaat gtgagcaggc atatttttca
    33721 aatcatcgca gatgacctgc atttgctcct ctaatccatt caaacggaca gagcgttcag
    33781 ccatatccgc caaacgctcc tgaatctcaa cagacaatat ctgtgcttga gtacgagtgc
    33841 tagcaaaaag ccccactgct ccattcccag cacagagatc cacaatcaac cccttcttag
    33901 gaaaacgtgg aaatcgtgat aagagaacac tatccaccga atagctaaaa acctctctat
    33961 tttgaatgat tttgatatct gtcgaaaaga gctggttaat gcgctctcct gattttaata
    34021 attgttcttc ttccatggtc ctattatagc aaattcatat taacattaca aaaaatataa
    34081 aactctaaac tacttcttct tttttaaatg gtgcagggct tctccagtcc agattggtag
    34141 cattcgtcga aagggagcaa agccgtagtt aaagcggtcg cttgaaaagc gtctccgtct
    34201 aggaaactgg tacttttctt cctccaaagt gcggatagaa agactggctt tccctgtaaa
    34261 ttcatctaaa tccactacct gaacttgaac ctcttcatcg actttcaagg tttcatgaat
    34321 attttcaata aatcctgtcc gaatctctga aatgtgaatc agccccgtat cacccgtctc
    34381 taactcaaca aaggcaccgt agggctgaat ccctgtaata cgccccttta gcttatcacc
    34441 gattttcatc ttagtcctcg atttcaatag tttcaattac aacatcttca actggcttgt
    34501 ccatagctcc tgtctcaaca gcagcaatgg catccaagac agcgtaagat gcttcatcag
    34561 ctaactgacc aaaaaccgtg tgacggcggt ctaggtgagg tgtcccacct tgattggcat
    34621 agatttctgc aatcggttct ggccaaccac cacgagtaat ttctttctta gaataaggta
    34681 ggtgttggtt ttgcacgata aagaactggc tgccgttggt atttggacca gcatttgcca
    34741 tggaaagagc accacggata ttgtaaagct cttctgagaa ttcatcctca aaagattcgc
    34801 cgtagattga ctcgccaccc ataccagttc cagttgggtc tccaccttgg atcataaagt
    34861 ccttgataat acggtggaaa atgacaccat catagtagcc atcttttgaa agagatacaa
    34921 agttagccac tgttttagga gcatgttcag ggaaaagctt gatacgtaag tctccgtgat
    34981 tggtcttaat agtcgcaaga ggaccttcta ctgtttcaat gtctacttgt ggaaaatgca
    35041 attctttttc taccatacca aatacttcta aggcagcaaa aatgccatct tcttctaatg
    35101 tttttgtaat ataatctgct ttttctttga ttttatcatg agaaattccc atggcaacgc
    35161 tgattccagc ataatcaaag agttccaagt cgttgagacc atctccaaaa accatgacct
    35221 tctctggttt caagccaagg tgttccacaa ccttttccac ccccgtcgct ttggagcctg
    35281 aaatcggcac aatatcagac gaatgttgat gccaacgaac catgcgaagt ttgtctgaga
    35341 gactgtcagg caagtgcaag tcatctccct tatcttcaaa agtccacatc tgatagatat
    35401 cttctttttc atggaaatcg ggatctacat ctaagtcggg ataaattgga ttgatagctt
    35461 cactcatcat atcggtgcga gtcgacaact tggcatcatg actcccaacc aagccatact
    35521 caattccttc ttgcttagcc caagagatat actcctcaac atctgacttt tcaatctgat
    35581 gctgataaat gacctgacct tttttatctt cgatataagc cccattcaaa gttacaaaaa
    35641 agtcaggctt gagatcacga atctctggaa caacaccaaa aatgccacgt ccagaggcga
    35701 ttcctgttaa aattcctttt tcacgcaact gtttaaaaac agtgggaatt gtagttggaa
    35761 taaaccctgt ctttgaattc cgcaatgtat catcaatatc aaaaaagaca atcttgatct
    35821 tctttgcctt gtatcttaat ttcgcgtcca tctcactacc tctttcaatc taactctttc
    35881 cattatatca taaagtaggc aaatccccta ttttcaaaaa gtttatcatt tttattttaa
    35941 tttcttggat gagaaaagag acatatttat gaaaaagctc catcgtgctt ttaatgtgtt
    36001 ctcttgtttt caaactcgta aaaagggagc cactgatcct aactcgctct ctcatttcaa
    36061 agcttgtgaa aaaagacccg ttggggtctt aattcgcttt cttgttttca agctcatgaa
    36121 aaagagaccc aactgggtct tttctttaat cttcgtttac gaaaggcatc aaagccatta
    36181 cgcgagcgcg tttgatagct gttgttactt tacgttggtt tttagctgaa gttcctgtta
    36241 cacgacgagg aaggattttc ccacgttctg aaacgaaacg gctaagaagc tcagtatctt
    36301 tgtaatcaac atattcaatt ttgtttgctg cgatgtaatc aactttttta cggcgtttga
    36361 atccgccacg acgttgttga gccatgtttt ttctccttta taagtttagt tgtccattag
    36421 aatggtaaat catcatctga aatatccaat gggtttgttg ctccaaatgg attttcatta
    36481 cgtgaaaagt ctggtactga atttgtaggt gctgaatagt ttgcagttgg tgcagagtaa
    36541 gctccacctg tgtgaccctc acgcacacta cggctttcca acatttggaa attctcagcc
    36601 acgacctctg tcacgtagac acgttgtcct tgctggttat cgtaactacg agtctggata
    36661 cgacctgtca ccccgataag tgagcctttt ttagcccagt tagcaagatt ttcagcctgt
    36721 tggcgccaca taacgacatt gataaaatca gcctcacgtt caccattttg actcttaaat
    36781 gtacggttta ctgcaagagt aaaagtcgca actgctacat ttgatggggt ataacgcaac
    36841 tcagcgtcac gtgtcatacg ccctacaagt acaacattgt taatcatagt ttaccttctt
    36901 acgcgtcaat tttgacgatc atgtgacgaa gaatgtcagc gttgattttt gaaagacggt
    36961 caaactcttt aagagctgca tcgtcatttg cttcaacgtt aacgatgtgg taaagtcctt
    37021 cacggaaatc ttggatttcg tatgcaagac gacgtttttc ccaagttttt gattcaacaa
    37081 cagttgcacc gttgtcagtc aaaatagagt caaaacgtgc taccaaagcg tttttagctt
    37141 cttcttcaat gtttggacga atgatataaa gaatttcgta tttagccatt gatatgttcc
    37201 tccttttggt ctaatgaccc caagactttg caaggggtaa gtgaggttcg ctcacaataa
    37261 actattatac tagaaaaaat ttttttacgc aagtaaaaac actagaattc gaaaaaacgc
    37321 cacatgggcg ttttcctgtt cttatggttt gatacggtgc aacatacgtg ggaatggaat
    37381 agcttcacgg atatgttttg ttcctgctgc gaaggttacc atacgttcga taccgatacc
    37441 aaatcctccg tgtggaactg taccgtattt acgaaggtca aggtagaatt catattctgt
    37501 acgatccatg ccaagttcat ccatcttagc gacaagggca tcgtaatctt cctcacgcat
    37561 agacccaccg ataatttctc catagccttc tggagcaagc aagtctgcac aaagcacgcg
    37621 ctctggattt ccaggaactg gtttcatgta gaaggccttg atggctgctg gatagttcat
    37681 gacaaatgtt ggcacaccaa agtggtttga aatccaagtt tcgtgtggtg acccaaagtc
    37741 atcaccatgc tcaagatgct cgtagtcagc atcttcatca ttttcatgct cttgcaagag
    37801 gtcaatggct tgatcgtaag tgatacgttt gaatggctct gcaatgtagc gtttcaagag
    37861 ttctgtatca cgttccaagg tttccaaggc ttgaggcgcg cggtcaagaa caccttgtag
    37921 aagagctttc acataagctt cttgcaagtc aagcgactca tcatgtgtca agtatgagta
    37981 ctcagcatcc atcatccaga actcagtcaa gtgacggcgt gtttttgatt tttcagcacg
    38041 gaaaactgga ccaaagtcaa agacacgacc aagagccata gcccctgctt ctaggtaaag
    38101 ctgacctgat tggctcaagt aggctggcgt tccgaagtag tcagtttcaa agagttctgt
    38161 agaatcttct gccgcatttc ctgaaagaat tgggctgtca aacttcataa aaccgttctt
    38221 gtcaaagaac tcataagttg catagataat agcgttacgg atttgcaaca cagctacttg
    38281 cttacgagag cgtagccaca agtgacggtt atccatcaaa aagtctgttc cgtgttcttt
    38341 tggtgtgatt gggtagtctt gagattcacc gatcacttcg atgtctgtga tgtccaactc
    38401 atagccaaat ttagaacgtt cgtcctcttt gacaatacct gtcacataaa cagacgtttc
    38461 ttggctcaag cgtttgataa catcaaactt ctcaagtccc acttcttcac caaatttttc
    38521 gacaaagttt ggtttaaaag ccacaccttg aaagaaggct gttccatcac gcaattgtaa
    38581 gaaagcgatt tttccttttc ctgatttgtt ggcaacccaa gcgccaatcg tcacttcctg
    38641 accaacatag tcttttacgt caataatcgt tacacgtttt gtcattattt ttccttttct
    38701 tttttattct ttatggcaaa ccacctctat attgttccca tccaggtcaa tcataaaagc
    38761 agcatagtaa atcggatgct cacttcgata accaggagcc ccattgtctc gcccacctgc
    38821 ctctaagcca gcctcataac aagcctgaac ttcttcctta ttttctgcta aaaaagcaaa
    38881 atgaacagga tcttgtgttc cctgagtcag ccaaaaatca ccaccaggat gagggctgtt
    38941 cggggataga aaactaatta gagaactagt cttaaaagcc aatttatagt ccaaaggagc
    39001 gagaaaactc ctataaaatc cttatgaaat ttgtaaatcc tttaccttaa tctcaaaatg
    39061 atcaatcatt ctcactaccc ataaatgctt tcaagcgttc gactgcttct ttaagcgtgt
    39121 ctaggtctgt cgcatagctg aggcggacat tttctggtgc tccaaatcca gctcctgtta
    39181 ccaaggccac ttcggcttct tctaagataa cagttgtaaa gtctgtcaca tccgtgtagc
    39241 ctttcatctc catggccttt ttgacatttg ggaagagata gaaggcccct tgcggtttga
    39301 ccacttcaaa tcctggtacc tctgcaagga ggggatagat ggtattaaga cgttcctcaa
    39361 aggcctgacg catgctttct acagtatctt gctcacctga tagagcctca actgctgcat
    39421 attgggctac tgctgacgga ttcgaagttg tttgacctgc aatcttggac atggcagcga
    39481 taatgtctgc ttctccaacg gcataaccaa tccgccaacc agtcatggca taagttttag
    39541 acacaccatt gatgaccact gtttgcttgc gaatcgcttc cgataggcta gaaatcggtg
    39601 tgaactcatg accattataa accaagcggc catagatatc gtctgctagg atgagaatat
    39661 cattttctac agcccagttt ccaattgcca agagttcctc acgggtgtaa atcatacctg
    39721 tgggattaga tggcgaattc agcaccaaaa ccttggtctt gtcagtgcga gctgcttcta
    39781 actgctctac ggtcacctta aagtgattgt cttccttagc agaaacaaag acgggaacgc
    39841 cttctgccat cttgacctga tctccatagc taacccagta tggggttggg atgatgactt
    39901 catcacctgg attgaccaca gccataaaga aggtatagag agaatatttg gctcccgcag
    39961 cgactgtcac ttgatttgac gctacagaat agccgtaaaa gcgctcaaag tagctattga
    40021 ccgccgcctt aagctctggc agacctgagg ttactgtata aaaagaagca cgcccatctc
    40081 gaatcgatgc aatggcggca tcttggatat ttttgggagt agtgaaatct ggctcaccca
    40141 aggttagaga caaaatatct ctaccctcag ccttcagtgc tttggcacgg gctccagcag
    40201 ccaaagtcac actttcttcc atttctaaaa cacggttgga tagtttcata ggccctcctt
    40261 gttgaccaat gctcctgttt caaaatctac tagataaaaa tcagatcctg acttaacttc
    40321 ccagattggc ttatcttgat aacggccaaa ggttatcttg tcaatctcgc cagctccctt
    40381 ttccttagaa accgtttctg ctttttcttg tgaaacaccc tgatttagct gataaacgta
    40441 aatcttatgg tcatctttac caatcaggac agcaagcgct tcttgctgtt tgttacgacc
    40501 aagaacgctg taataagatt ccaagccatt gtataaatca acctgatcag cctgctctaa
    40561 tcctgcatac tgctgagcta atttttctcc ttcactttta gctgtttgat agggtttcat
    40621 gctaagagaa accatataca gaaaggaacc actgataacc acaaacaaaa tcgtcatccc
    40681 tagaccatac tgccacagta gattattttt tgctttgttt tgtctttttt tcactcgtct
    40741 attttaccat ctattaagct ttattacaag tgaatataag aatactcttc gaaaatctct
    40801 tcaaaccacg tcagctttat ctgcaacctc aaagctgtgc tttgagcaac caattctatt
    40861 tctcccttca aacaaaaccg attttgaaag tgaaacagtt cttacttttt cagtcacaaa
    40921 tgattagagt ttccgaaatt ttacaatttt caaataaact ctgagcaaat ttcttgcaag
    40981 ttcttttgtt ttgttgtaat atattttata acaacgagag agttctcgaa attttaagaa
    41041 aaaggagaca catcatgtct aaaaaagtat tatttatcgt cggatcacta cgtcaaggtt
    41101 ctttcaacca ccaaatggcg ctcgaagctg agaaagcact tgctggtaaa gcggaagtta
    41161 gctaccttga ttattcagcc cttcctctct tcagccaaga tttggaagtt ccaacacatc
    41221 cagctgtagc tgctgctcgt gaagcagttc tcgttgcgga tgctatctgg attttctctc
    41281 cagtctacaa cttctctatc cctggtacag tgaaaaactt gcttgactgg ctatctcgtg
    41341 cccttgactt gtctgataca cgtggcgttt ctgcccttca agacaagttt gtcacagtat
    41401 catctgtagc caatgcaggg cacgatcaac ttttcgctat ctacaaagac ctcttgccat
    41461 ttatccgtac acaaggcgtt ggtgatttca ctgctgcacg tgttaatgac tctgcctggg
    41521 cagacggaaa attggttctt gaagaaacag tcctaaactc acttgaaaaa caagctcaag
    41581 acttggtcga agctatcaag taactaacac tcaataaaaa tcaaaaagca aactaggaag
    41641 ctagccgcaa gctactcaaa gcactgcttt gaggttgtag atagaactga cgaagtcagt
    41701 aacatatata cggtaaggcg acactgacgt ggcttgaaga gattttcaaa gagtataaca
    41761 taataaaagc gaacaagact acttttctga cactcagaag actagcttgt tcgctttttt
    41821 ctcatttata aactaaaata gctgatgata catattgcca aatacaaaat atctgatttc
    41881 tatacttcct ttacaaaaac caatccctgt ggttgtgaca aatcttctcg gtaaacaaat
    41941 ccagcaaagt tcaagcgacg tgcttccccc acagtttgta cttgattttc tattaaagct
    42001 gttagaaagg ccccgcgcgc tttcttggag atagttgagt gaatcttcag ctgaccgcct
    42061 ctatcctcca tgaatttgaa ggtcaccatc tttgctctga tttccttaga aaatacagtc
    42121 tcaaactctg atgacaagag agagaaaatc acttcttcct tcttcagagt ttcatcatag
    42181 gctgccttcc aatggctctt caaagtctta ccagcgactt ttaatttcat caaaaaatcc
    42241 aaacggtgag gagccatggg tgacaagact ggaacaacac cgtacaaagc cgaggtaatg
    42301 aaaacatgat tttcaagata atcttgttcc gcctcggtca gcttatctct cttaatgttg
    42361 cggtacataa gcccatcaaa aagtttcaag gctggatagt gttgagcagt ttgccttttc
    42421 aaagcttgga tattttgaaa ttcttccgcc gctttctcag ctgatacctt gtagaaactc
    42481 tccaattgac tggcagaata gagagccaag gcatcaagca cggcctgact ttctggtttt
    42541 aaaggaattg cctcgatact tgggaagtct gtgttcattt cttttgctgt tgggattaaa
    42601 attttcatat tgatagtgta gcatattttt tagccacaac caagaaattc atttgaaaaa
    42661 cctcggtttg accgaggttt ttctatttat ttgggccatc ctaaccagac agccacgaga
    42721 acaagggctg tgccagctaa acttgttaag atagataaga atttatcttt tttctccttg
    42781 aactgagaaa aacctatctt caaagcgagc agtccgagta gcattgaagc aaccatcaaa
    42841 taaaaaccca tttctttgtc ctccattagg cttaatttac cttattataa catatacttt
    42901 ctgaaaaaaa cctattttta ccgtagctta aaggtcttga gataattgtc ttttcctact
    42961 tgaccttcga aggttttacc attttcaagg taaggaaggt catcggatac tgaagccttg
    43021 accttgtata tcttgccatc aactttaaag aagtagacag tgtctccctt gataacagct
    43081 gatttgaggt ctgctactac tccattgatg ctttctgtcg ttgcattgtc aatttcaagg
    43141 tcgtttttat tggcatactt gctgagcatc tcttccactg tagtagcaac gataacattt
    43201 tggtactcga ctgcgtctac cagggcgtac tctttgacca agccagcatt gtccttcaag
    43261 cccatgatgt agagaggctt gtcattgagg ttgatgagga ttgggaaggt tgctttgtag
    43321 gatttctcct gaacagcacc ttctgctgat tcacgggctg attcttctgt cgcagaagcc
    43381 aagctatact tagtgatttc tcctgttcgc atattttcaa ggatgaaacc aagattactc
    43441 tcatccgcat tagccgacgt cacacctgtg tagagataga tgtcattacc gatagacaag
    43501 taattatagc cattggtagt ctgggtcacg tttttcttgg aaatcatggc attcaagaaa
    43561 ccgtccttgt acttgccgtt gtagttgatt tgctcaatgg tttcctctgc tggatagatc
    43621 ctgtccaccc attctggaac atctgataag ctgtattcct tggtttctcc atttgtagca
    43681 tccaagataa tgactgaagc aggacgagga acagcaagtc caaattgctt ttggtaaacc
    43741 gttgctacat agaaaggatt gccctcatcg tccacctcaa aagatggagt tttaaagatt
    43801 ttggtcgggt acttcaagcg caggtgacgt ttgacatcac ggttaaaata ctccgagtct
    43861 gaatacttga ttggtgtctt caagtccacc aaatccgcat ttccagttac catgtccacc
    43921 ttaatatact caccgattcc cttggcttga ttgttaaacc atttgatagg gtctgcatat
    43981 tctagtggtg tgacccgata aggtttccca tcaattgtca attgggtata ggtatctgcc
    44041 gctacgtatt gcgacacctt atcggttagg gaacccaagt agcggtctcc aattttttca
    44101 gcagtacttc tatctaggat aggaacctta ctggtgtcac tcttaggaaa ttcagtaaag
    44161 tctttttccg taaccgtgac tacattggca taatttttag cctgaaacat gctggaagtt
    44221 accaaggaaa ccaaagctgc caagagaaga attcctccaa tcgaagctaa cagaattttc
    44281 cctaaccgat tgattttgaa accctcaaga tttaaggcag cttccgcctt accgtggcgc
    44341 acatgaaccg ttttgacaag gtttatcccc ttgccaaagc caaataatat tgccacaact
    44401 aacaaatgcc cacagaggaa gaagagaaat tcccagctgg tcaggttaag gggcggtaaa
    44461 aagatatacc aggtcgttgc gataaaaata agttcaaaga ctattcgttt catttgcttt
    44521 cctcctaaat gattcgtcct tccaagtgat ccagctcatg ctggcaaatc tgagctggga
    44581 agcctgtcaa ggtaatggtc tgttcctgcc acttgctgtc acgataggca agccttatgg
    44641 tttcataacg cttagttgat ctcacaccta ccaaggacaa acagccttct tctgcctcat
    44701 aggatccttc aaaggacaga agcactgggt taaacatgac cacaggaacc aagccaagat
    44761 taaagataat cacgcgcttc tgcaccccaa tcatattggc agctagacca acacaggtat
    44821 cacgatttgc taagagtgta tcctgcaaat ctctggcaag ataaaggtcc tcctgacttg
    44881 ccggctgaga cacttgagat aaaaataaga tatccttcac aattttcttt tccacttcct
    44941 cacactcctc ttgcttttta ctatattata gcaattatag cacaaaagcc gataactgca
    45001 aaccctttcc ctcaactgta gcaaaaagcc atccgaagat gacttttttg ctatttaatt
    45061 tctgtataag ttacttccaa gccacgctta acagctggac gattggcaat tttttctgcc
    45121 cattttacta gattttgata acttgaggca tccaagaatt ttgcagaacc ttggtaaaga
    45181 tttccttgaa ctaactgtcc ataccaagac cagatagcaa tatctgcaat cgtatagtca
    45241 ttgcctgcaa tataaggttt ctgagccaat tccttatcca ataaatccaa ctggcgtttc
    45301 acttccatcg taaaacggtt aataggatat tccaattttt caggagcata attgaagaaa
    45361 tgtccaaatc ccccacctag aaaaggtgct gcacctgctt gccagaatag ccaattcaaa
    45421 acttctacct tttccacagg attacttggt aaaaaggctc caaatttctc agcaaggtaa
    45481 agaagaatat gagcagactc aaagactctt acgttttcag tacctgactg gtccaataag
    45541 gctggaatct tggaatttgg attgagcttc acaaagtctg atccgaattg atccccatcc
    45601 atgatagcaa tcttatacaa gtcgtaagcc gcttccttaa aaccagcttc tagtaattct
    45661 tccaataaga tagtaacctt cacaccattt ggtgttccca gtgaataaag ctgaaaagct
    45721 tgttctcctt ttggcaagtt ttgttcgaaa cgggcacctg ctgttggtct gtttagcccc
    45781 gtaaaagctc cttgattact agcttcatcc tgccatacgg tcggtaattg atatgctgac
    45841 atccgaaacc tcccttaaat cgcattcttg tcaaaaccga gtttgcgttg aataaactta
    45901 acgatttcga cgatgataat cattgagaag cttccagcca taacaattcc ccattgtgac
    45961 aagtctagtt tggttacgtg gaagattcct tcaagcggtt ctacaacgat tgttgccatg
    46021 agaaggataa aggataccaa gatggaccag ttaaaggtct tagacttgaa tgggccaact
    46081 gtcaagatgg attggtagac agacttgaca ttgtaggcat ggaagagctg aatcaaacca
    46141 agggttgcaa aggccatcgt tagggcatct gcatgaatag catgattgtc acccacatga
    46201 actgggtaag caatcgcaag gccataaaca ctcataacaa gagctgcttg gagtacacct
    46261 tgataaatga tagaactcaa aacaccacct gagaagaagc ttgccttgcg tccacgtggt
    46321 ttatgattca tgacaccagg ttccgcaggt tcaacaccaa gagcgatagc tgggaaggta
    46381 tccgttacca agttgatcca caaaagatga accggctgta agacatccca accaaacaag
    46441 gttgatagga agatggttaa tacttcagca gtattagcag aaagtaggta ctgaatagtc
    46501 ttttgaatgt ttgagaagac cttacgtcct tcttccactg cgacgataat agtcgcaaag
    46561 ttatcatctg caagaatcat atcagaagcc cccttagaaa cctctgtacc agtgattccc
    46621 ataccgatac cgatatcggc tgttttcaga gctggcgcgt cattgacacc gtcacctgtc
    46681 atggcaacga ctttaccttg tttttgccaa gccttgacga tacgaacctt gtgttctgga
    46741 gacacacggg cataaacaga gtattgacca acgacttttt caaattcttc atctgacagt
    46801 tcattgagtt cagcaccagt taaaacgtga ccttctgtat cgtttgcgtc aatgattccc
    46861 aaacgtttgg caatggcttc cgctgtgtct tggtggtcac ctgtaatcat aattggacgg
    46921 attcccgctt ccttagccac acgaacagcc tcagcggctt caggacgttc agggtcaatc
    46981 atcccaatca aaccagtaaa aattaaatca ttttcaagct cttcagaagt gagattttct
    47041 ggaatactat cgataatctt ataagcacct gcaaggacac gcaaggcttg atgagccatt
    47101 tcagaattgt ttgtacgaat gagatttgta accttctcat caatcggagc aatatcccca
    47161 gccttatcac gaagaagaca acgttttaag agttggtctg gcgcaccctt gactgctaca
    47221 aggaaacgac catctggcaa tgggtgaact gttgacatga gcttacggtc agagtcaaat
    47281 ggcaattcag ctacacgagg atatttctct aagaaacctt tgacatcata gcccttgtcc
    47341 aaggcatatt ggataaaggc tgtttcggtt gggtcaccaa tcaagttacc ttccacatcg
    47401 attttcgtat cattggccaa gacaactgaa cgaagtagtg gcatttcaag acctagttca
    47461 atatcatcag ctgagtcatg tagaaccgca tcgtagaaga ctttttcgac tgtcatcttg
    47521 ttcatagtca gcgtaccagt cttatcagaa gcgatgattt cagttgaacc aagtgtttca
    47581 actgctggca acttacgaac gatggaatgt cgtttggcca aaacttgagt accaagagaa
    47641 agaacgatgg taacgatagc aggaagtcct tctggaatgg ctgcaacggc aagggcaaca
    47701 gaagtcaaca actcaccaag tggatttttc ccttgaatga agacacccac tacaaaagta
    47761 acaagggcaa tgaccaagat agcataggtc aagaccttag aaaggttgtt caaattttgt
    47821 ttgagtggtg tatcagtctc atccgcatct tgaagcatac cagcaatatg accaacttca
    47881 gtgtacatac ctgtattgac aacaacaccc atcccacgac cataggttac gtttgagttt
    47941 tggaaggcca tgttgacacg gtcaccaata ccagcatctg tcgcaagctc gactgacaag
    48001 tctttttcga ctggtacaga ttcacctgtc aaggctgctt cttcaatttt aagagagttg
    48061 gcttctatca aacgtaggtc cgctggtacc acgtcacctg cttcaagggc aacgatatcg
    48121 cctggtacca attctttaga gtcaatctct gccatgtgtc catcacgaag aacgcgggca
    48181 actggactag acatggattt gagggcttca atagcttctt cagcttttcc ttcttggtaa
    48241 acaccaaagg cagcgttgat gataaccaca gctaggatga taatggcatc tgcgatatct
    48301 tccccaccag aagtcacgac tgacaagatt gctgccgcaa ctaggatgat aatcatcaaa
    48361 tccttaaatt gctcgatgaa tttgaccaag attgatcgtt tctcgccttc ttcgagttca
    48421 ttgtgcccaa attcggcaag gcgcttttcc gcctcacttg atgacaaacc ttgctcggtc
    48481 gcatccacag cctgcaagac ctcttcaggg ctctgagtat aaaacgcttg gcgtttttgt
    48541 tcttttgaca tgtgtctcct ccttgacatt gtgtgcaaaa cagactctct ttctgtcata
    48601 gcttttcacg acaaacaaaa agaaacctgt taatcataac aagtctcgct gtttaagata
    48661 gggccggaaa gcatactttt cagcataaaa ttcggaatga cgacactatc acaggtttct
    48721 gccagctact cccttgagta gtaccattat accaaatttt ggggagtttt caaagagtaa
    48781 aaactgcctt atttgaattt ttccttgaaa accagtataa tggtagaatg ctatgtgact
    48841 agaaaggaag ttgaatgaag caatctatct caaatctcaa gttagctgag cgtggagcca
    48901 ttatcagtat ttcgacctat ttgatcttgt ctgcagccaa attagcagct ggtcatctcc
    48961 ttcattcatc cagtttggtg gccgatggtt ttaataacgt atcggacatc attggaaatg
    49021 tggccctctt aatcgggatt cggatggcgc gccagcctgc agaccgtgac caccgttttg
    49081 gtcattggaa gattgaagat ttggcaagct tgatcacttc tatcatcatg ttctatgtcg
    49141 gtttcgatgt tctaagagat accattcaaa agattctcag tcgggaagaa acggtcattg
    49201 atcctcttgg tgcaactcta ggaatcattt ctgcagcgat tatgtttgtg gtctatctct
    49261 acaatactcg cctcagtaag aaatccaact ccaatgcgct gaaggcagct gctaaggaca
    49321 atctttctga cgctgttacc tcacttggaa ccgccattgc catcctagct agtagtttca
    49381 attatccgat tgtggataaa ctggttgcta tcatcatcac tttctttatc ttgaagactg
    49441 cctatgatat cttcatcgag tcttccttta gtctttcaga tggctttgac gaccgcctgc
    49501 tcgaggacta ccaaaaggct atcatggaaa ttcccaaaat cagcaaggtc aaatcgcaaa
    49561 gaggtcgcac ctacggtagc aacatctacc tggatattac actagagatg aatcctgact
    49621 tgtctgtttt tgaaagccat gaaatcgcgg atcaggtcga gtctatgctg gaggagcgtt
    49681 ttggcgtctt tgataccgat gtccatatcg aaccagcacc tatccctgag gatgaaattt
    49741 tagacaatgt ctataaaaaa ttgcttatgc gtgaacaatt gattgaccaa ggaaaccaac
    49801 tagaagaact cttgactgat gattttgtct atattcgcca agatggagag cagatggata
    49861 aagaggctta taagaccaaa aaagagttaa attctgctat caaggacatt caaattactt
    49921 ccatcagtca aaaaaccaaa ctcatctgct atgagttaga tggtatcatc cataccagta
    49981 tctggcgtcg ccacgaaacc tggcaaaata tctttcatca agaaaccaaa aaagaataga
    50041 gaaatccttt catgagacgg gatttttcta ttcttttata ctcaataaaa atcaaagtgc
    50101 aaattaggaa gccggtcaca ggctgtactt gagtcggcaa tgtgaagccg acatagtttg
    50161 cactttgatt ttcgaatagt cttaactatc aaattcactg agatactcat agcgttcgta
    50221 tttttcaagg agtgcttcat ttttctcatc caattctttt tggagagtag ccagcttacc
    50281 aaagtcagag ccgttagcct gcatttcctc ttcaatagca gcgatacgtt tttccaaggt
    50341 ttcaatatca ccttcaatac ttgcccactc ctgcttttct tggtaggtca tgcgtttctt
    50401 gtcttctcga accttgacca ctttttcctt ttcggccttt tgcacttgat tggccatatc
    50461 tgtttcaaaa gctttttcat caagatagtc ggtgtaatga ccaaagaaag gacgaatctt
    50521 gccatcctca aaagcgagaa tcttggtcgc taccttatcc aagaaatagc ggtcgtgact
    50581 gactgttaaa acgggacctg caaaaccttg caagaaattc tctaagactg tcaaagttgc
    50641 aatatctagg tcattggttg gctcgtctaa aagaagaaca tttggttttt ccaaaagcag
    50701 tttgaggaga taaagacgtt ttttctcacc ccctgacaat ttctcaatca aagtcccatg
    50761 cgtcgaacgt gggaagagga attgctccag caactcagcg atggaagtcg tagaaccacc
    50821 actggtcttg acctcctctg ccacttcctg caggtaattg atcacacgct tgctttcatc
    50881 caaaccctca atttgttgag agaaataggc gatgcgaaca gtttccccaa tcacaacttg
    50941 tcctgctgtc ggctcaagac ttcctgcaat caggttaagt agggttgatt ttccaacacc
    51001 attgtcccca acaattccaa tacggtcttt agcctgaact aagagattaa aattttgcaa
    51061 aatgggctta ttttcatagg caaaggaaac atcctgaaac tcgatgactt tcttcccaat
    51121 ccgactggtt tcaaagttca tagtcaagtc tgtctcagca ctactgcctg aaacttcctt
    51181 tttcagatca tggaaacgat tgatacgagc ttgttgcttg gtcgcacgcg cctgcggttg
    51241 tctgcgcatc caggccaatt cttgtttgta gagttgttct tttttgtgaa gaagagccgc
    51301 gtcgcgctca tcctgttccg cctttaggcg aacatagtcc tggtaatttc cctggtactc
    51361 ggtcaagcct gcacgatcca actcgaaaat ccgtgttgac aaagcgtcta agaaataacg
    51421 atcgtgagtg ataaaaagga cggtcttctt agaatttttc aaaaagaggg tcagccactc
    51481 aataatcgca atatccagat ggttggtcgg ctcatccaaa agcaagaggt cgtggttgcc
    51541 aagtaagact tgtgccaact gtacccgtct tctcagacca cctgacaatt ccccaacagg
    51601 agtagataag tcttgaatgc ccaatttgct aagaacggtc ttgacctgac tttcgatttc
    51661 ccaagcttgg agagagtcca tctctgccat gacacgttcc aaacgcgcct gcttgtcctc
    51721 actatagtcg agcataatca attcatactc acgaatgagc tggatttcct tgagttcact
    51781 agatagaacc gtatccaaaa ctgtctttct atcatcaaaa tcaggatcct gagtcaagta
    51841 accaatctgg taatcatttt tagctgaaaa aggactgaca tccccatcaa atccagaaac
    51901 accagaaagg acgtccaaaa gggtggtctt gccagtccca ttgacaccga ttaaaccaat
    51961 tctgtctaag tcatggataa taaaggaaat atccctaaaa acggtcttgt caccaacgga
    52021 tttacttagt ttttcaacga taaaatcact cattttttct ccctcaggta agcatggatg
    52081 gcttcacgat tattctccaa ttctccatcg acaatggcaa actcaatctc tgttaaaatc
    52141 tctcccaagt ctgggcctgg ctgatagcca tattccttga tcaaaatacc gccattaatc
    52201 tgaatctctt tcttgtcatg gatagtcaag ctttggtatt tttctgtgat ggcttgtggg
    52261 ttgacttctt ttccttgagc ttgacgaaga ttttcagcct gtaaaagcaa atctatgtca
    52321 aagcgataac aatctcgctt gctcaattct ccattttcac gcagagccaa aataatcagc
    52381 aaatcctgaa cttgcttggc aaactggcgt gaggtcttcc aagatttcaa aaatgactgc
    52441 gcattttcaa tctccaaagc ccatagtaaa gccgcccagg cttgttcaga ggattcaaaa
    52501 gtaaaatcag tctccaaatc aaacagtctg ttgagcttgt cctggctaga tgccatatca
    52561 gggagatagt cataagcttg actctcaatc atggaagcca agccccttct ccaaaatgga
    52621 gccagcaaga gtttatcaaa ctcgacgaag gtacgctcta cagaaatttt ctccaaaagc
    52681 ggcgtcaagg tcttcatagc tttaaatgtt tctggctcaa gtgcaaaacc aagactagcc
    52741 tgaaaacgga aaccacgcat aatccgtaaa gcatcttcgt tgaaacgctc actagccact
    52801 ccaactgctc gcaagacttg cttttccaaa tcttctaaac catggaacaa gtcaacgatt
    52861 tctcctgtct catccaaggc aaaggcgttg actgtgaaat cacggcgttt gaggtcttct
    52921 tctagcgatc gtacaaagga aaccgcactg ggtctgcgat agtccacata gacatcctct
    52981 gtccgaaagg ttgttacctc atactcctca tccccatcta agaccaagac ggttccatgc
    53041 tcgattccga tatcggctgt tcgcggaaaa atctgcttgg tctcttctgg ataagaagac
    53101 gtcgcaatat ccacatcgtg gatagggcta tggagaaggg catctcgaac agagccccca
    53161 acaaaataag cctcaaagcc tgcttcttta attttttcta atactggtaa agccttctga
    53221 aattcagaag gcatttgcgt taatctcata ataagtgttc taatccatag acaagctcat
    53281 gacgcttgac aacttcttta attcccaaat tgactcctgt catgaaggag atgcgatcat
    53341 aggagtcatg acggagggtc aacccttctc cctgattgcc aaagatgact tcctgatgag
    53401 ctaccaagcc tggcaaacga actgagtgga tgcgcatacc atcaaagtca gcaccacgag
    53461 caccagcaat cagctcttcc tcatctgctg caccttgctg aattgactct cgaacctctg
    53521 ccatcaactc agctgtttta atggctgttc cactcggagc atcctttttc ttgtcatgat
    53581 ggagctcaat aatctccaca tttgggaaat atttggcagc ctgcgtcgca aattgcatga
    53641 gtaagacagc acccaaggca aagttagggg caatcaggcc acccaagtct tgggcacgag
    53701 aaaattcttt tagctctgca atttcttcac tcgtgaaacc agtcgttcca actactggag
    53761 caaagccatt ttcaagagca aaacgtgtat tttcgtaggc aacagctgga gtagtaaaat
    53821 ctacccagac atccgcttca aaaccagcta aatcagcctt atccttgaaa acaggaatac
    53881 cctgccattc tgactcagac tcaaaaggat ccaaaactgc caccaagtcc aagtctggat
    53941 cagtcaatac catctgacaa gcagcctggc ccatctttcc cttaaaaccg gcaataatta
    54001 ctcgaatact catctctact cctgtctaag atacaaagtc cgtaagaaca caaagtgaaa
    54061 ataggaattc caatcaagaa gtgtctactt cttggaagaa ctatcttttt cacacagggt
    54121 tccaggcgtg ttcaattatc aagatacaaa ggaccttagc tgcctctgaa aaatagggaa
    54181 tggcactgac tttccacgaa aggcaagaca ggcatctttt ttcaagaggc aggtagtccg
    54241 tgttcaattt ctaagataca aggcatctta actagcctag aagcgccaac taaatcactg
    54301 gaatataacc cagagcaata cttcctgctc ctaggtgcgt tccaatgaca ctaccaaatg
    54361 tagcaagtga aacatccgaa cccaagccaa aatcaagcaa gtgctgacgc aattcttcag
    54421 ccttttcagg agcattccca tgaatgacaa tgacccggta ttgacctgaa gccgttgttt
    54481 ccttgataat ttcaattaag cgcttggtgg ccttcttttc agtacgaact ttttcgtaaa
    54541 cttcaatcac accttgatcg ttaaaataaa ggattggctt aatgctaagc aaattgccca
    54601 aaatggcagc cccatttgaa aggcgtccac cttttaccaa atgatccaag tcatctacca
    54661 tgataaaggc tgacgtacgg ctgatttgaa tggctagctt atcctgaatg ctggcaaaat
    54721 catcgccctg atcacgccaa ttaaagacgc tttcaaccat gatgcctagg ggagcacttg
    54781 taatcaaagt gtctgggaaa gcaatggtta agccctcata gtcatcgacc atatactgga
    54841 tattttggta aaaacctgaa attccagaag ataggaaaag ccccaaggca tgtgtatagc
    54901 cttgttcttt gagcgaagtt aagatctcat ctaacttggc aatacttggt tgactggtct
    54961 taggcaattc agaagcctga gccatttttt ggtaaaattc ctcagcagac agattgatgc
    55021 cttcgacata ttcctcacca tcaatattga caggaatatc caagacaaac aagtcttctc
    55081 tttgcaagat ctctgcactg agataagcag aggaatctgt gaaaacagct aatttcatat
    55141 tagaactcca aattaattcc tggtaagtct aatgcaattt cagtcacttc gtaagtcaaa
    55201 cgattgagca tgttcaaaca tggacgagcc aaggtttcca cctcttcttg gttcaattca
    55261 cttggttcat tgacaatacg gccatcgata tggtttactt gtgagattgt tccactaatg
    55321 acaaacttat caaatacaat cataaagctc aagatgacaa tcaaggaagt cacttgattt
    55381 tcttggtcat gttggagcaa ttggaaattc acatccacct tggtttcagg agctccattt
    55441 tcattttccc attcaaaatt acgcgcatca aaatgatact gactaacaaa ttcttgttca
    55501 cgtttaagat tcatgtcttt ctccatcggc tacaatatta taagctattg taccataatt
    55561 ttttattttc atctagtttt ctaggattta gtcaatccca atttcagcac gaactacatc
    55621 tgtgatggta tcaacatagt agtttacttc ttctgttgta ggcgcttctg ccataacacg
    55681 caagaggggt tctgttccac ttggacgaac aaggatacgg ccgttccccg ccatttcttc
    55741 ttccatcttc tcgatgatgg ccttgatagc tggcacttcc atggcctttt ccttcatgac
    55801 gttttccact cggatattaa ctaatttttg tggataaatc gttacttctg ccgccaactc
    55861 tgataagctc ttaccagttt ccttcatgat tttagtcaat tgaactgctg ataattgacc
    55921 atcacctgtg gtattgtaat ccatcaagat aacgtgacca gactgttcac caccaaggtt
    55981 gtagcctgat tttctcattt cttcaacaac gtagcggtca ccaactgcag taactgcctt
    56041 gttaatacct tcgcgattca aggccttgtg gaaaccaagg ttagacataa cagttgtcac
    56101 aattgtattt tgagccaatt gtcctttttc agaaaggtat tttccgatga tgtacataat
    56161 cttgtcacca tcaacgatgt caccattctc atcaacagca atcaagcggt cactgtctcc
    56221 atcaaaggcc aaaccaatag ctgacccact ttctttgacc acttcttgaa gggcttctgg
    56281 atgtgttgaa ccaacattaa ggttgatgtt aagaccgtct ggtgtttccc cgataaccgt
    56341 caattgggca ccaaggtctg caaagatttg acgggcactg gtagaagctg ctccattagc
    56401 tgtatccaag gcaaccttca ttccatcaag aggagttcca gttgaaacaa ggtatccttc
    56461 atacttacgc aagccttctg gataatctac caaaattcct aagccttctg cacttggacg
    56521 aggaagagtg tcttcctcag catctagcaa ggcttcaatt tctgcttctt tttcatcatc
    56581 tagtttgaag ccatcaccgc caaagaactt gattccgtta tcaagggctg ggttgtggct
    56641 agcagaaatc atgacaccgg cacttgctcc ttcagtttca accaagtaag ctactgctgg
    56701 tgttgcaagg acaccaagtt tgtatacgtg aatccctact gaaaggagac ctgccaccaa
    56761 ggccgattcc aacatttccc ctgaaatacg tgtgtcacgt cctacaaaga ctttcggcgc
    56821 ttccgtttca tgttgactaa gaacatagcc tccaaaacgt cctagtttaa aggctaattc
    56881 tggtgttagt tctaggttag cttctccacg gactccatca gtcccaaaat atttacccat
    56941 tgttataaaa tccttttcta tttttaattc gtttttgaac tagttgcttt cgttgacgaa
    57001 gatgtctccg atgaactgct tgtacttgaa tttgatgtgc ttgaacttgg tgctactggt
    57061 tttgtagtca ccttcattat tgtatcaaac ggagtgataa ctgccggtaa gacaacacca
    57121 ttgcggtcga ttgcctgcaa aggtactgaa ccactgtaat tacctgttat acgttcgcta
    57181 gttggcaaaa cagcgataat cttatcaatt ctatccaatg tctcttggtc actcgtaata
    57241 gacacttctt tatctgacac catgacattt tcaatttgta cccgactatc aatttgacta
    57301 gggtcaatct ctggtacaat ctttacctta tccttctgag ccttcttacc aatcttgact
    57361 gtaatttttt gcggagtcgc cacagcggtc agcccattgg gtaaatcttc aatgctcaaa
    57421 ggaacttcaa tcgttccaac accggcatct gttaggtcag cagtaacctt gaatttacgt
    57481 gtactttctt gcatttcact agctagcgat aggcgatttg caccagtcaa gaccactgat
    57541 acttctgaag caaaaccgct aataaaatac ttatcactat tatagcgtat gtcaataggg
    57601 acatttgtta ctgtattagt ataggtttcc gtttttacct gcctagcact ggtactgttt
    57661 tgaaaattcg tcgccgtagc atagacaaat aagacacaag caaaaaagag tgaggatatg
    57721 atatataaac tatttttttt catgtttcca tcctcctagc aatcgttctt taaaactaag
    57781 acccacttcc tcttttggaa gtaagatttc acgtaattct gtttcaaatt catcaagtgt
    57841 taggttgtgc ttaaaccttc cattataggt tatcgaaatt cctcccgttt cctctgatac
    57901 gacaaaagtc aaggcatctg agacttctga taaaccgata gccgcccggt gtctggtccc
    57961 aaattccttg gaaatccctg tgttttttgt caagggcaga taggcagacg tcacagcgat
    58021 acgttcttct ttgataatca ccgcaccatc atgtagggga gtgttgggaa taaaaatgtt
    58081 aatgagaagt tctgcagaaa tcttagcatc caagggaatt cctgtcgaaa tatactcctg
    58141 caaggtacgt acacgctgaa tagcaaccaa ggccccgatt ttacgaggac tcatgtattc
    58201 aacagactta acaaaggcac gaatcatctg ttcctcagca ctaatagggg cattggaaaa
    58261 gaaatctgtc gctcttccca aacgttccaa accagtccga atctctggag agaagataac
    58321 aaccgccgca ataaccccat aagtaataat ttgattgatt aaccaagaaa tcgtagtcaa
    58381 accaatcata tttgcaagga tttgagctaa aataaacacc aaaactccac gtaccaaaat
    58441 cataatcttg gttcctgcaa tagcttttgt aaaatggtat aaaatataag caacaatcaa
    58501 aatatcaatc agattgatag ctatcgtcca tggacttgca aacaaactgg tccaatattg
    58561 cagattggat aattgttgaa aattcatccc tgatatcctc cctatcaaaa cactttcgtc
    58621 ctattatacc attttctggc atttttttcc ctatcctagt ccattttaca ttgaacaaaa
    58681 atatgataaa ataaactgac taaaaaaaac aaaggagaaa ctatgtctca actctatgat
    58741 attaccattg tgggtggtgg tcctgtcggg ctttttgcag ccttttatgc ccacctacgc
    58801 caagccaagg ttcaaatcat cgactctctt ccccagctag gtggacaacc tgctattctc
    58861 taccctgaaa aggaaatcct agacgtacca ggcttcccaa acctgactgg agaagagttg
    58921 actaaccgct tgattgaaca gctaaatgga tttgataccc ctattcatct caatgaaacg
    58981 gttcttgaga ttgacaaaca agaagaattt gccatcacaa cttctaaagg aagtcacctg
    59041 actaaaacag ttatcatcgc tatgggtggc ggtgccttca aaccacgtcc gctggaactt
    59101 gaaggggttg agggctatga aaatatccac taccacgttt ctaacattca gcaatacgct
    59161 ggtaagaaag tgacgattct tggtggggga gactcggctg tggattgggc tttggctttt
    59221 gaaaaaatcg caccaactac ccttgttcac cgcagagata atttccgtgc cttggaacac
    59281 agtgttcaag ccttgcaaga atcatctgta accatcaaga caccattcgc ccctagccaa
    59341 ctccttggaa atggaaaaac acttgataaa cttgaaatca caaaagtcaa atctgatgaa
    59401 actgaaacca ttgacctaga ccacctcttt gtcaactatg gtttcaaatc ttctgtcggt
    59461 aaccttaaaa actgggggct cgacctcaac cgtcacaaga ttatcgtcaa cagcaaacag
    59521 gaatccagcc aagcaggtat ctatgctatc ggtgactgct gctactatga cggaaaaatt
    59581 gatctgattg cgacaggcct cggagaagct ccaactgctg tcaacaacgc tatcaactac
    59641 attgaccctg aacaaaaagt acaaccaaaa cactctacta gtttataaaa aagaaccacg
    59701 agtcacatag gattcgtggt tttataattc atccgctatc ttattgattt ttctgagtct
    59761 gtgattgaca ccacttttgg tcagaggggt gctgagacta tctgctaact gctggataga
    59821 gtagtctggg tgctgaatcc tcagttgcgc cacttcctgc aaatctactg gcaaattttc
    59881 taagcccatg atatctttga ttttactgat attgttaatg gtcttcatgc tggcagaaac
    59941 tgtccgagcg atattagctg tctcggcatt attagcccga ttgaggtcgt tacgggtttc
    60001 tcgcaaaatc ttaacccgct caaaatcatc acgtgcctgc atggctccta ttactatcaa
    60061 gaagtccata atgtcttctg ctcgctggag ataggtcaca gcccccttct tgcgctcaag
    60121 caccttggca tccagtaaaa actgttggag aagggaggca attccttgcg cgtggtccag
    60181 ataaacagaa ctgatttcca actggtactt gcctgactca gggtcacgaa tgctcccatt
    60241 tgccaagaaa gcgccacaga gataggcacg acctgcttcc tcatccgata aaatcgcctc
    60301 atcaatacct gtttccaggc caaagaaaga gtctgccaag tgcaaatcac ttaacaaatc
    60361 ctgcaccttt tcatctgtaa aaacggtata gacgcgattc ttgcgaagat tgctccgttg
    60421 gtggtgacga atttcagatt tgatttcata gagatggaga aaggactcat agaggtgacg
    60481 ggccagtttg gcattttctg tcacaactga caaagtcaag cccgaagtcg agagaccgat
    60541 gctaccagac attttgataa tggcagataa ttcatgccag ctcagatggt gttggcccag
    60601 gatttcttct tttactgcta ctgtgaaact cattttttca cctgtataat gcgcatcaac
    60661 tcgtccacaa tcaaatctcc atcgtggaag gcaccgccat tttccagacg aaggaagtta
    60721 gatgaaatca cgcgcgaaac ttgcttacaa agacctacaa aatcgtgttc cacttgcact
    60781 aagtattcat caaaacggtt ggaattcatg tattcctgag gcactttttc aatattcacc
    60841 aagacagtgt cgataaaagg gcgaccaagg tgacgatgca agacttccac gtggtcgcta
    60901 tctgtaaagt gttccgtctc cccacgttgg gtcatgatat tgcagacata ggcaatttct
    60961 gccttggttt ccaaaagagc ccgcccaatt tccttaatca cgatattggg caaaatagag
    61021 gtaaagaggg aacctggccc taggacaatc atgtcacttt caaggatggt ctgcactact
    61081 cgacggctgg ccagaggcgt atcatcgttt agggcattgg tcacatagac attgtcaatt
    61141 atgcctcgat ggtctacaat atgactctct ccagccactt ctgtcccatc ctgaaagact
    61201 gcatgaaggg tcaaaggatg gtcactggaa ggataaattt tccctgttgt atggaaaaat
    61261 ttgctcaata actgcatggc attataggtt gaaccctgca tttctgacaa gccagcaatg
    61321 atgagatttc ccaatggatg gccagcaaag gctccggcat cctcagagaa ccgatactga
    61381 aagaccttct cataaaactt aggcatatcc gacatggcca caaggacatt acgaagatca
    61441 cctggcggtg tcaactgttg catatttttt cggagttcac ctgaagaacc accatcatct
    61501 gccaccgtca cgatagctgc gatttccaca tctttttccc gcagactttt tagaatgacg
    61561 ggacttccag tccctccacc aatcaccgtt atctttggtt ttctcatgaa cggtttaccg
    61621 tttcctttct gcggtctttg tcgcgatgcc cttcattaac agaccaattc ttggataagt
    61681 cctgcgccaa gcgtttagca aatgccacac tacggtgttg tccacccgta catcccatgg
    61741 caatggtcaa aacggactta ccttcctttt ggtaacttgg cagaatcggc tcaatcaagg
    61801 ccaataaatg ttgataaaag tcttctgact caggatggtt catgacataa tcataaacag
    61861 gttcatccac acccgtttgg tttctcagtt ctggtaaata atagggattt ggcaagaaac
    61921 ggacatcaaa gaccaagtcc gcatcaatcg ggattccata cttaaatccg aaagacatga
    61981 cttcgatacg gaaagactgg gcttgttctt ggtctgaaaa ctgctctgca agggttttgc
    62041 gcagctcacg tggagtgagt tcagtcgtat ccaccacatt ttggctcata tttttcaaag
    62101 gtgccaagag ttcacgttcc aacttgattc catctaaaat acgaccgtct gctgctagtg
    62161 ggtgactccg tctggtttcc ttgtaacgag cgaccaattc cttatcagcc gcatccaaaa
    62221 agaggatttt gaaatccaaa ccatcttgat tttccaactc atccaaaaca gcttgaatct
    62281 ctgaaaagaa agaacggcta cgcatatcca ctaccaaggc caacttagga ttgtcttcct
    62341 taatttcaac cagctgcaaa aacttaggca agagagctgg cggcatatta tcaatggtga
    62401 aataacctag atcctcgaag gactgaatgg ctacagtttt ccctgcgcca ctcatccctg
    62461 tcacaatcac caagtgaagt tgtttctttg tcatcttttt ctccttatat caaaagaagt
    62521 ttggcaacac caaacttcaa ctagcttatc caatctctgc gatgacttca atttcgactt
    62581 ttacatcacg aggaagacga gctacctcca cagctgaacg agctgggaat tcctctttga
    62641 aggccgtttg gtaaacctca ttaaaaggaa caaagtcgtt catatcgctc aagaagcaag
    62701 ttgttttgac aacatggtca aagtctgttc ctgcttctgc caaaatagca ccgatgtttt
    62761 tcaagacttg ctctgtctgt tcttggatat tctctcctac aatttcccca gtttcagggg
    62821 ataggggaac ttgaccgcta gcaaacaaaa ggttgccaac gatttttcct tgaacatagg
    62881 gtccgatagc ctttggggcc ttatctgtat gaattgtttt tgccattttc ttttcctcac
    62941 aatttttcta agattgcatc ccaagcctca tccatccctg ccttactgac agatgaaaag
    63001 aggatgaaat cgtcactcgg gtcaaagttt aatttctttt tgattgctga ttcatgcttg
    63061 ttccatttac cacgaggaat cttgtccgcc ttggtcgcca caatgatgac tggaatctca
    63121 taatacttga gaaattcgta catctgcaca tcatctgctg acgggtcatg acgaaggtca
    63181 actagactga caaccgcacg gagattttcc cgagtcgtta agtactcctc aatcatgcac
    63241 ccccactttt cacgttcctt tttagaaaca cgagcatagc cataaccagg cacatccaca
    63301 aagcgcatct tgtcatcaat gttaaaaaag ttcaggagct gggttttacc aggttttcct
    63361 gatgtacggg cgagattctt acggttcaac atagtgttga taaagctgga tttaccaaca
    63421 tttgaacgcc ctgctagggc aatctctggc agttcatcct gcggatagtg ggacttatta
    63481 gctgcactga gcaagatttc agcattgtgt gtattaagtt ccatagtcac ctctaggctg
    63541 tttctaggat cggtttatcc gttccatcta cagtttcttt agtgatgcga accaatttca
    63601 cattttcctg actcggcacc tcaaacatga catctagcat ggtttcttcg atgatggagc
    63661 gaagtccacg cgcccctgtc ttccgttcga ttgctttatt agcaatctct tgaagggctt
    63721 cgtcgtcaaa ttccaactca acatcatcat aagaaagcaa ggtttggtat tgtttcacca
    63781 aggcatttct tggctctttc aagatgcgaa ccaagtcatc aacggtcaat tgctcaagag
    63841 ccgcaaaaac aggcaagcgt ccaatcaact cagggataat accaaatttt tgaatgtctt
    63901 cagcgatgat ttcttgcatg tatgagctgt tttcgtcaat cgccttatta ttttgaccaa
    63961 atccgatgac tttttcaccc agacgttgtt tgacaatttc ttcaatacca tcaaaagcac
    64021 cacccacgat gaagaggata ttttttgtat ccacttgaat catctcttgt tgtggatgtt
    64081 tgcgtccacc ttgaggcggt acgctagcaa cagttccctc aataatcttg agaagggctt
    64141 gttgcacccc ttcaccagaa acatcacgtg tgatagacac attctcactc ttcttggcaa
    64201 tcttgtcaat ttcatccaca tagataatgc cacgctctgc acgttcgatg ttaaagtcag
    64261 caacctgcaa gagtttgagg aggatatttt ccacatcctc acccacataa ccagcctccg
    64321 tcagagctgt cgcatccgca atagcaaaag gtacattcaa gctcttagcc aaggtctggg
    64381 caaggaaagt tttccctgaa ccagttgggc caatcatcaa aatgtttgac ttctgcaaat
    64441 ccacatcttc tgactcttcg cgtgtatcgt ggaaattgat gcgtttgtag tggttataaa
    64501 ccgccactgc caaggcacgc ttggcacgat cttgaccaat tacatagtgg ttcaagatat
    64561 ggaggagttc aattggtttt ggcacctcag acaagtctgc caagacttcc tcaaccaatt
    64621 cttctcgaat gatttcctga gctaactcca cgcattcatt acaaataaaa gcattgttgc
    64681 cagcaattat tttttgtact tcttcttggt ttttgccaca aaatgagcaa taaaccatca
    64741 tatcattttt tctatttgta gacatgattt ccttccattc tatactgtca ttctatctaa
    64801 aataaggtca tgtaaaaagc atgaatacta ttgaccagat tggtaaaggc atttaaccaa
    64861 aggaggatag aaagcccgta acgcttttta cgaaaagctt gtgctcctgc cagaaagcag
    64921 atgaaacaca gaaaagccgt gaatagacca aataaactcc gttccattag acttcctttc
    64981 tcttgcggta ttggatggta aaatcataag gattcttctc atctttggcg taaaatttgc
    65041 ttgaaactgt ctcaaaaaga gacaagtcaa gttcttcagg gaaataggta tctccttcca
    65101 cccgagcatg aatgtgagtg acaatcactt catcaaggta aggttcaaaa gcctgaaaaa
    65161 tttgcttccc accgataatg tagagattct tttcttgagc ctgataccag tcaagaacag
    65221 actggacgtc ctgaaaagta gcaaccccat ctatcttttc ttccggatta cgcgtcaaaa
    65281 tcaaggtttc ccgttttgga agcaagcgac gccccatccc atcaaaggtc acacgcccca
    65341 tcaagatagc atgattcaga gttgtttctt taaagtgctg caattctgct ggcaaatgcc
    65401 aaggcagacg attttcctta ccaatcacac cctcttcatc ctgggcccaa atagctacga
    65461 ttttcttagt catgcttcca tccttttcac tgatagtact attttatcaa aaaactcaaa
    65521 aaaagactgg tttggaatag cttacaaaat agaaaaaatc tgtaagaaat ttcctacaga
    65581 tttatctatg tttccttatt tcttacaaac caggtgcttg tccaagttcg gctgcaagca
    65641 tccaaattgt tttatctgtt tcagttttag cgcctgcaaa gataccgttt gtcacatcgt
    65701 caccttcttc atcagtgaca tccaaacctt tttggaaaag ttctgacaag taacggtaga
    65761 taacaagaac acgttccaag ctttcttcaa cattacggta ttcaccagct tcttcttcga
    65821 tttcactatt ttgaaggaac tctgtcaatg tagagaatgg gcttccaccg agtgtaatca
    65881 agcgttcact gatttcatcc aattgaccgt caagagcttc catgtactca tccatttttg
    65941 gatgccatac aaggaaacca cgaccatgca tataccagtg cacttggtgc aaagcaacgt
    66001 gagctacata caaatcagca acagcttggt tcaagacttc ctttgttttt gccaatgcta
    66061 ctggcgctgc ttttgtcaat gatgttacgt cttttactgc ttcttttttc aattctacca
    66121 ttttctttac ctcattcatt attttttgta accacttctt aatgattact accttagtat
    66181 actactaagg aaaaccaata gcaagacaaa gaacttatat gagaaaagtt gcaatagact
    66241 gataatttaa gaattcttta gaagacaaaa agagggttgg atgcttatag tcaatggaaa
    66301 tccatcagca aactaggaaa ctaaacccag cttgaccaaa gcacaagttt tgaggtcgca
    66361 ggtagaactg accaagctga tgtggtttga agaaattccc caatactata atagattgaa
    66421 ataagatgaa accaagtcga ttaggaaagg taaatgaatt tctaaaatgt tttacaaatc
    66481 acaaggtatt tgtctatatt caatctacta tataagcttc atttccgagc aagaacttga
    66541 atttactcga ggatgaacta tcataaaaca aagaaaaaga gggtgctact atcacatccc
    66601 tctttcaaat catcgcttaa taccaaacgc tgacatctac gcgcccttgg attcctttca
    66661 tgtattcaga agaggtatat tgccaacctt tttttcctga ataatgaggg ttttcccatt
    66721 ctaaagcatt cgtataggcc gctacccagt ttacatgttt taaaatatct gggtgtttta
    66781 aacgcgtctg taataaacta cgatagctat agacatacac attttgataa cccgcctgct
    66841 tcatcgtgtc catgtacttg ttgatgattt taacccaagt gcctgtatca cttggagctc
    66901 tcttgctctt atttacatat tcccaattct caacatcata atagataggg taagacaggt
    66961 tcatattgta tttctttata agttcaatgg tctgtttagc gtcactctca gcatcggtct
    67021 cattttcagc ataggtatag agatagacac cataaggaat tcccagacgg tttaactcct
    67081 taatgttatg cgccaattcc ttgtcttctt taccgctata acctagacga acaatgacac
    67141 catccacttc gttctcatca ataacctttt tccaatcatt gatacgacca ttgtgctcac
    67201 taatatcaat gactttctta gcatgttcgg ttcccacttg ttcttctcta ttattaaaga
    67261 aatagcgctt accatctctg tgaacccagc cgacattcaa atcttttttt tctttgagct
    67321 caccagaggc cgcaaagata tagcgagtac catccataat cacttcgtca gtcgccatgg
    67381 caccacttcc agtcaaatag tagttgcctt gccactcatt ccgagccata tagccccact
    67441 tcttgaaata gtaccatttg ccgcccactt tttgccactc ttggttagca taagttccat
    67501 cggattttag ataaaaataa gcagaatagg ctggatcata gagccattca ttttgcatca
    67561 tagctccttg accattcaag aaataacttc cttgccattg gcttttagcc atgtaacccc
    67621 acttcttgaa gtagtaccac ttatttccaa tcaattgcca ttcttgatga gcataagccc
    67681 catctgactt gagataaaac caactcttgt aattactgtc atagatccac tcgttttggg
    67741 ccatatatcc acctgatttc aggtagtaat ttccatgcca ttcattctgc gagtaacgac
    67801 catctgaatt aatataaaac caagccttat agtagttgtc aaaaatccat tcactttgag
    67861 ctttagaacc atcatccttt acataataat ccccatccca gtgggcagca gcattagtct
    67921 gtacttctgc gctcgtttgc gttttattag aagactgatt ttgctctgaa ctacttgaag
    67981 ctgtcgttgt atcctgcgaa gtttttgcta cttcagtttc atttgcagcg acatgactag
    68041 ttgccaagcc tagtaaacag atacttgcta atccaatttt tgttttcaat ctttctctcc
    68101 tataaaaaat gtaacagaca tctgaatgct gttccaccta gcttttgcta cttactgatt
    68161 attttacaaa gtcaagcaaa gccaagaagc tttcagcttc aagtgacgca ccacctacaa
    68221 gggcaccgtc aacgtctggg caagccatgt atgaagcaac attttcaggt ttaacagaac
    68281 caccgtattg aacacgaact ttgtctgcga cttcttgacc aaagtcagca gctacaacgt
    68341 cacgaacaac tttacacatt ttttgtgcat cgtcttgtga agctgattta ccagtaccga
    68401 tagcccagat tggctcataa gcgataactg aggcagcaac ttgttcagca gtcaatccag
    68461 ccaatgcagc agatacttga gcacctacga attcagcagc tttaccagct tcgtaagttt
    68521 caagtgattc accacaacag atgattggaa gcataccgtt cgcaaagatt gcttttgctt
    68581 ttttgttgat atcttcatca gtttcatgga agtagtcacg gcgttctgag tgaccgataa
    68641 caacgtagtc agtaccgatt tctttcaaaa cttgtgggct agtttcacca gtgaaagcac
    68701 ctgcattttc aaagtagcag ttttgagcag caactttaag gtttgagcct tttgcaacag
    68761 caagaacagt tgtcaaatca agagctggag cagcgatacc tgcttcaaca agatctgatg
    68821 aaggaagttt tgatgcaact gcttcaacga atgctttagc ttcttctgga tttttgttca
    68881 ttttccagtt accagcgata aatggtttac gtgacatttc acatacctct tttttcaatt
    68941 tattttctat ttattctatc acaattatac acagcttgca aatcttactc ggatttcaag
    69001 cctacttgca tggattaatc cttccagaga tccatggcat ttatgaaatc tgcagatacc
    69061 tgtgtcaact gaggattgct tgcttctctt tctgctctct tggcctcaat ttgagccaca
    69121 ctcttgattc cttcatggcg ccagtttctc aaaatcgcct gaatgtactt ccagtttggt
    69181 tttccattca aaacagcttc acgaagagcc tccttaatca agtcagcact ggttccatct
    69241 tcctttagtg tcttggtcaa atcctcaatc tcaaaaggcg tcaacaatcg tcccaactcc
    69301 tgctggaagg tttccaccaa atccttgagc tggttttgag gtgttagctg gtctgaactt
    69361 gaatgaactg ctccaaacag gtcatccaaa cgttccaagg ccaaactagc atcaaagagc
    69421 aattcaattt cgccatttaa ttcgatagta cgatactgga gcagtcccct ttccgtcaga
    69481 ttagaaatgg actggttgac atccgaaatt tccttgccaa tcctttcagc aatctggctt
    69541 ggcgacattt cttctaagcc tgtcgtattt tgcaaataga aaaattgcca aaccagaaaa
    69601 tcgtcgctag aaggaaagag ttccttaaaa tgcaagagca gggcactcgg taaaaccaag
    69661 gtacctgatt taaaagcgtc taaatatgtc ataattcctc ttataaatac ccatatgcag
    69721 atgcatcatc ttctatcttt ctccaatcaa aaggcgtctc ctgatagacc gcatggttta
    69781 cccaattact gaaaaagagg gctgcagatg aagaccaaca aagacaaggt acctgattaa
    69841 catcatcatc tttaaagtaa ttttctggaa tatgtgggtc gaaacctgca tcacgatctc
    69901 gaaaatactc ttttgccaag gtatcacggt catactccaa atgaccaaaa ctataaattt
    69961 ctcgtaaatc acgactggcc aaaatagaaa ccccaacctg aggtccttct gataaaatct
    70021 cgagattggt cttgtttaag acctcttcct tagaaatctc cgtgtgccgt gaatgagggg
    70081 atacatagct atcatcaaag cctctaaata gaaggtgacc ctcttttagg gtgtcctgag
    70141 gataaatacc tgatagctta ctgtccatct ggtatttttc tacaccatag cgcagataaa
    70201 gcccagcctg agccccccaa cagatatgaa gggtcgaata gacatgagtc ttagaccact
    70261 caagcatctg tctaaattcc tcccaatagt ccacttcctc aaatggtaaa tgctcaactg
    70321 gagcacccgt gatgatcatc ccatcaaaat actcatcctt gacttcagga aaagttttat
    70381 agaaggtctc catgtgctct gaacgagttg ttttagaacg gtggctctcc atatagagaa
    70441 aatcaatatc cagttgtagg ggtgtattag ccaagtggcg caacaactgg gtctctgtga
    70501 ccattttctg tggcatgaga tttaaaatta aaatcttcaa aggacggata tcttggtggg
    70561 cagcacgttg atcatccatg acaaagatat tctctgtccg taaaatctca acagctggca
    70621 attttttatc aattcgaatc ggcataaccc tctcctaact gtactctttc aggaataatt
    70681 gcatatgttt gaagacaaat ctcaaacact tagtcctttt attatactgc aagaagatat
    70741 agttttcaat tatagttttt ctctaactag ttatagtcta tttttatatc ctagtgtaga
    70801 gaaaacagcc ctagggactg ttttcattaa taatgcataa gaactttgta gtcgtagtca
    70861 ccaatttttt cacggccgtt caattcatcc aattcaacaa ggaaggcaca acctgccata
    70921 acaccaccaa gtttttcaat catctcgata gttgccttaa cagttccacc tgtcgccaaa
    70981 aggtcatcta caataagaac acgttgacct ggcttaatgg catccgcgtg catagtcaag
    71041 gtatcgacac cgtactcttt ttcatagtca gcagaaataa cttcgcgtgg caatttacct
    71101 ggcttacgaa caggcgcaaa accaattccc aactcaaagg caactggaca acccacgata
    71161 aatccacgag cttcaggtcc cacgatcatg tcgactttct tgtcagtagc atactgaacg
    71221 atttcacgaa cagcgtagct ataagcattt ccatcagcca tcaaaggact aatatcacgg
    71281 aaggtaatgc cttcctttgg ataattttca attgttgcaa tgtaatcttt taaattcatc
    71341 tttttctttc tttcaaagtt ttttactctc tattatagca tattttttaa gaaagaaaaa
    71401 aggaaaagtt aacttcaata attatctaac gttttgacga tttataacta gccatcgcaa
    71461 taaagcccaa tttctgttta ttcttagcaa acattttata catagttaaa aactgctttc
    71521 tattctcctt tttacaagca tttacacaaa ttttcaaagt tcctagcaaa ccttcgtcat
    71581 aaatcatacc cgataatttc attaatgtca tttcaccagt caatgctttc acatcacaat
    71641 aacctgattc tatcatcacc tgttcccaac catcttgagt taaaggacct acatttacat
    71701 gaattgcttg tgataattcc tgtctgatag actctttagc ttccttaaga agcacatcat
    71761 gtgtcaagag aagacctcca ggttttaata cccttagata ttccattaca cattttttct
    71821 tagcttgatc ggcttgcata gtcagcatag cttcatttat aacaatatca aaactagcat
    71881 cttgataagg aagtttcatt gcatttgctc tttcaaaact gattaaatga gcaacacctg
    71941 ccgttccagc agatttttta gccacttcta aagcttgagc atccatatca acagcagtta
    72001 tcttgcaacc aaaacgctgt gccaactcaa ttgctgtagt tcccctatta cacgcaacct
    72061 ctagtattct cttttctttt gaaaatcctc cttctgcaat taaccaatct gtggcacgct
    72121 ttccacctgg acgtaagcgt tttttcccca attttgctaa aaacttatga cctgcttctg
    72181 acatttgaac acctctgttt tttcttcatt ataacacaaa atgatattat ctgacaattc
    72241 taatctatta tttgataaac tagaatcaaa agcatacaag tcaccataac tatagcataa
    72301 atttattatt acctctctag ttcgaattca attgctcacg tagtttacgc atatagcttc
    72361 acttgtagat ggtacccaca aataatactc aatggaaatc aaagagcaaa ctaggaaact
    72421 agccgtaagc tgctcaaagt acatctttga ggttgcagat aaaactgacg aaatcagtaa
    72481 tcatacgtac ggcaaggcga cgttgacgtg gtttgaagag attttcgaag agtattaaag
    72541 agcatcacaa tatagatgca taatatgact tctctagcaa aaaggagaga agcattttgt
    72601 ttctctccct agattaactg atatctctcc actacaattg ataaagagat atcttttctt
    72661 gtagtgcttt gaattttatt tattttagta gattgttttt tcagtttcta catcgaagaa
    72721 gtgtgctttg ttcaagtcaa atccaagctc aactgttgca cctgtttgca agtagtcacg
    72781 agcatcaact tttgcaacaa actcgtcttt accaacttga cagtaaaggt gagattctga
    72841 accaagcagt tctgatacag agatagtcgc ttttacaaca cagtctggga atgtttcaag
    72901 gaaagcaggt tctgcattca cgtcttctgg acggatacca aagatcaatt cttttccttc
    72961 gtagcctttt tcacgaagaa ctttcaatgc tccttctggc actttcaaac ggaaaccgtc
    73021 agaaacaatt tcgctaccaa ccaatttcac ggtgatgaag ttcatagctg ggcttccgat
    73081 gaatcctgca acgaatttgt taactggatt tttgtaaact tcttgaggag taccgatttg
    73141 ttctacacgt ccgatagtac ctgtaccagc agggttctta gtagctgaca taataacgat
    73201 acggtctgca agtgtcatcg cttctgtttg gtcgtgagtt acatagatag ttgtagctcc
    73261 gatacgacgg tgaattttag cgatttcagc acgcattgat acacgaagtt tggcatccaa
    73321 gtttgacaaa ggttcgtcca tcaagaatac tttcgcatca cggacaatcg cacgccccat
    73381 ggcaacacgt tgacgttgac cacctgaaag gtcagctggt ttacgttcca agaattcttt
    73441 caatccaagt atttcagctg cttcttgaac acgtttgtta atgtcttctt tgctgtattt
    73501 acgcaatttc aaaccgaaag ccatgttgtc ataaacagtc atgtgtgggt aaagagcgta
    73561 gttttggaat accatggcga tatcacggtc ttttggagct acgtcgttga caactacgcc
    73621 atcgatagat gcagtacctt ctgtaatgtc ttcaagacca gcaatcatac ggagtgtagt
    73681 tgatttacca catcctgaag gtcctacgaa aacgataaat tctttatctt tgatgttcaa
    73741 gttgaaatct tcaactgaat agtgttcgct gtttggatat tttttgtaaa tatttttaag
    73801 attcaattct accatgaggt gaactccttt tgtcttttga taattatatt atatatgaaa
    73861 acgctttata tttctatggc aagatgacca aaaaaataaa caagttctgt gcaacttgca
    73921 caaaacttta gagaatatcg gagaatttga agaagataat ctttattatt cttctataga
    73981 caaaagcaga aacaaggatc agttttccca tattttcggg ctctataata tttgtagtgg
    74041 gtaaatcccc tatggatatt atggagc
//
LOCUS       DAVKNL010000002        66121 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVKNL010000002 DAVKNL010000000
VERSION     DAVKNL010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 66121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66121
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.002"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(370..2097)
                     /gene="ezrA"
                     /locus_tag="VW411_000076"
     CDS             complement(370..2097)
                     /gene="ezrA"
                     /locus_tag="VW411_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEU9792892.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEEKKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2179..4125)
                     /gene="gyrB"
                     /locus_tag="VW411_000077"
     CDS             complement(2179..4125)
                     /gene="gyrB"
                     /locus_tag="VW411_000077"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEU9792893.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEIVGDTDKTGTTVHFTPDPKIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4140..4703)
                     /locus_tag="VW411_000078"
     CDS             complement(4140..4703)
                     /locus_tag="VW411_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9792894.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDISNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4788..5342)
                     /locus_tag="VW411_000079"
     CDS             complement(4788..5342)
                     /locus_tag="VW411_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEU9792895.1"
                     /translation="MVKVAVILAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5353..6576)
                     /locus_tag="VW411_000080"
     CDS             complement(5353..6576)
                     /locus_tag="VW411_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU9792896.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6613..9063)
                     /locus_tag="VW411_000081"
     CDS             complement(6613..9063)
                     /locus_tag="VW411_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEU9792897.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLRKKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTEASLEPRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEDFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPSVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9263..10192)
                     /locus_tag="VW411_000082"
     CDS             complement(9263..10192)
                     /locus_tag="VW411_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU9792898.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELIVAVNALMHQDMMDRIKPFLNFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARSLIQEGNFSH
                     AQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVR"
     gene            10332..10619
                     /locus_tag="VW411_000083"
     CDS             10332..10619
                     /locus_tag="VW411_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEU9792899.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10653..11987)
                     /gene="ciaH"
                     /locus_tag="VW411_000084"
     CDS             complement(10653..11987)
                     /gene="ciaH"
                     /locus_tag="VW411_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEU9792900.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(11977..12651)
                     /gene="ciaR"
                     /locus_tag="VW411_000085"
     CDS             complement(11977..12651)
                     /gene="ciaR"
                     /locus_tag="VW411_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEU9792901.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(12760..15306)
                     /locus_tag="VW411_000086"
     CDS             complement(12760..15306)
                     /locus_tag="VW411_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU9792902.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLAAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15429..15869)
                     /locus_tag="VW411_000087"
     CDS             complement(15429..15869)
                     /locus_tag="VW411_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEU9792903.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQAVCIVEITKVSVKPFNRVSADHAYKEGE
                     GNKTLVYWRQVHEDFFRDCLGEAGLTFTPESKVVLEEFRKVYPL"
     gene            complement(15844..16713)
                     /locus_tag="VW411_000088"
     CDS             complement(15844..16713)
                     /locus_tag="VW411_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEU9792904.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNILLQS
                     CQEFVKDYLDTICSFYLGKEVWYRFTELTNTEANCLVGTKEFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSVIFPFVNDADQLKQAITVLRQQGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKIDFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(16691..17191)
                     /locus_tag="VW411_000089"
     CDS             complement(16691..17191)
                     /locus_tag="VW411_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9792905.1"
                     /translation="MEIKNHFGVYCVCFENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELKNFMVHHVIALYDVEMNESAPQV
                     TISEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVND
                     EKSTCP"
     gene            complement(17202..17900)
                     /locus_tag="VW411_000090"
     CDS             complement(17202..17900)
                     /locus_tag="VW411_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEU9792906.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     RFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(17893..18159)
                     /locus_tag="VW411_000091"
     CDS             complement(17893..18159)
                     /locus_tag="VW411_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEU9792907.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            complement(18225..19151)
                     /locus_tag="VW411_000092"
     CDS             complement(18225..19151)
                     /locus_tag="VW411_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEU9792908.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFNLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(19226..20041)
                     /locus_tag="VW411_000093"
     CDS             complement(19226..20041)
                     /locus_tag="VW411_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9792909.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(20045..20365)
                     /locus_tag="VW411_000094"
     CDS             complement(20045..20365)
                     /locus_tag="VW411_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU9792910.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            complement(20707..20787)
                     /locus_tag="VW411_000095"
                     /pseudo
     CDS             complement(20707..20787)
                     /locus_tag="VW411_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(20831..22828)
                     /gene="metG"
                     /locus_tag="VW411_000096"
     CDS             complement(20831..22828)
                     /gene="metG"
                     /locus_tag="VW411_000096"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU9792911.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            complement(22952..24211)
                     /locus_tag="VW411_000097"
     CDS             complement(22952..24211)
                     /locus_tag="VW411_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9792912.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNVDEIANIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(24213..24914)
                     /locus_tag="VW411_000098"
     CDS             complement(24213..24914)
                     /locus_tag="VW411_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9792913.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(24898..26097)
                     /locus_tag="VW411_000099"
     CDS             complement(24898..26097)
                     /locus_tag="VW411_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU9792914.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVARADRHINELNQARNEAASAPAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            26362..27708
                     /gene="gor"
                     /locus_tag="VW411_000100"
     CDS             26362..27708
                     /gene="gor"
                     /locus_tag="VW411_000100"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEU9792915.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
     gene            27905..28441
                     /locus_tag="VW411_000101"
     CDS             27905..28441
                     /locus_tag="VW411_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEU9792916.1"
                     /translation="MKKAHVYAIPAIGAALIAALAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALAVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            28492..28578
                     /locus_tag="VW411_000102"
                     /pseudo
     CDS             28492..28578
                     /locus_tag="VW411_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(28647..28862)
                     /locus_tag="VW411_000103"
     CDS             complement(28647..28862)
                     /locus_tag="VW411_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEU9792917.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            28960..29280
                     /locus_tag="VW411_000104"
     CDS             28960..29280
                     /locus_tag="VW411_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9792918.1"
                     /translation="MRHKFQQVLNKIHDFLNGYDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFSKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(29311..29649)
                     /locus_tag="VW411_000105"
     CDS             complement(29311..29649)
                     /locus_tag="VW411_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9792919.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(29661..30380)
                     /gene="trmD"
                     /locus_tag="VW411_000106"
     CDS             complement(29661..30380)
                     /gene="trmD"
                     /locus_tag="VW411_000106"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEU9792920.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(30370..30888)
                     /gene="rimM"
                     /locus_tag="VW411_000107"
     CDS             complement(30370..30888)
                     /gene="rimM"
                     /locus_tag="VW411_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEU9792921.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELVLFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPSKRV
                     DVEILEGLDDED"
     gene            complement(30919..31707)
                     /locus_tag="VW411_000108"
     CDS             complement(30919..31707)
                     /locus_tag="VW411_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9792922.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFEDVSIEAHIPVFVFCQP
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELLE"
     gene            complement(32284..32523)
                     /locus_tag="VW411_000109"
     CDS             complement(32284..32523)
                     /locus_tag="VW411_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEU9792923.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            complement(32543..32815)
                     /gene="rpsP"
                     /locus_tag="VW411_000110"
     CDS             complement(32543..32815)
                     /gene="rpsP"
                     /locus_tag="VW411_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU9792924.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            32985..33194
                     /locus_tag="VW411_000111"
     CDS             32985..33194
                     /locus_tag="VW411_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9792925.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTTET"
     gene            33673..33886
                     /locus_tag="VW411_000112"
                     /pseudo
     CDS             33673..33886
                     /locus_tag="VW411_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            33970..34773
                     /locus_tag="VW411_000113"
     CDS             33970..34773
                     /locus_tag="VW411_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU9792926.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVIFHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(34829..36370)
                     /locus_tag="VW411_000114"
     CDS             complement(34829..36370)
                     /locus_tag="VW411_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9792927.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(36372..36854)
                     /locus_tag="VW411_000115"
     CDS             complement(36372..36854)
                     /locus_tag="VW411_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU9792928.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPGIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKVRN
                     "
     gene            complement(36854..37939)
                     /gene="rlmN"
                     /locus_tag="VW411_000116"
     CDS             complement(36854..37939)
                     /gene="rlmN"
                     /locus_tag="VW411_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEU9792929.1"
                     /translation="MKPSIHSLVHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERISHIVVM
                     GIGEPFDNYNNVLNFFRTINDDKGMAIGARHITVSTSGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALE
                     LTELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKECVLAFYDTLKKKGVNCVVRQEHGT
                     DIDAACGQLRSNTMKRDRQKAVAAVNP"
     gene            complement(37960..38472)
                     /locus_tag="VW411_000117"
     CDS             complement(37960..38472)
                     /locus_tag="VW411_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEU9792930.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDDK
                     GQRHFVIRQK"
     gene            complement(38628..39233)
                     /locus_tag="VW411_000118"
     CDS             complement(38628..39233)
                     /locus_tag="VW411_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEU9792931.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            complement(39406..40443)
                     /locus_tag="VW411_000119"
     CDS             complement(39406..40443)
                     /locus_tag="VW411_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU9792932.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQMLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKAFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKANSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            complement(40477..41412)
                     /locus_tag="VW411_000120"
     CDS             complement(40477..41412)
                     /locus_tag="VW411_000120"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEU9792933.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            complement(41965..43155)
                     /locus_tag="VW411_000121"
     CDS             complement(41965..43155)
                     /locus_tag="VW411_000121"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEU9792934.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAADLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            complement(43370..44713)
                     /locus_tag="VW411_000122"
     CDS             complement(43370..44713)
                     /locus_tag="VW411_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9792935.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            complement(44853..45668)
                     /locus_tag="VW411_000123"
     CDS             complement(44853..45668)
                     /locus_tag="VW411_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU9792936.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLSEQGKNYYWTWAYNHIGYNRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAILNWK"
     gene            complement(45720..47900)
                     /locus_tag="VW411_000124"
     CDS             complement(45720..47900)
                     /locus_tag="VW411_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9792937.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFAPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVALEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            complement(48186..49112)
                     /locus_tag="VW411_000125"
     CDS             complement(48186..49112)
                     /locus_tag="VW411_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9792938.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
     gene            complement(49105..49797)
                     /gene="ftsE"
                     /locus_tag="VW411_000126"
     CDS             complement(49105..49797)
                     /gene="ftsE"
                     /locus_tag="VW411_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEU9792939.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            complement(49815..50910)
                     /gene="prfB"
                     /locus_tag="VW411_000127"
     CDS             complement(join(49815..50837,50839..50910))
                     /gene="prfB"
                     /locus_tag="VW411_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEU9792940.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQDKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            complement(51018..51674)
                     /locus_tag="VW411_000128"
     CDS             complement(51018..51674)
                     /locus_tag="VW411_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9792941.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKISILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            complement(51982..52692)
                     /locus_tag="VW411_000129"
     CDS             complement(51982..52692)
                     /locus_tag="VW411_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9792942.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVASGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGRIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            complement(52692..53456)
                     /locus_tag="VW411_000130"
     CDS             complement(52692..53456)
                     /locus_tag="VW411_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9792943.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            complement(53456..54412)
                     /locus_tag="VW411_000131"
     CDS             complement(53456..54412)
                     /locus_tag="VW411_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU9792944.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKSPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            complement(54416..55285)
                     /locus_tag="VW411_000132"
     CDS             complement(54416..55285)
                     /locus_tag="VW411_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU9792945.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            complement(55553..56713)
                     /locus_tag="VW411_000133"
     CDS             complement(55553..56713)
                     /locus_tag="VW411_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9792946.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKNNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            complement(56814..57062)
                     /locus_tag="VW411_000134"
     CDS             complement(56814..57062)
                     /locus_tag="VW411_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEU9792947.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            complement(57141..57731)
                     /gene="clpP"
                     /locus_tag="VW411_000135"
     CDS             complement(57141..57731)
                     /gene="clpP"
                     /locus_tag="VW411_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEU9792948.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            complement(57905..58534)
                     /gene="upp"
                     /locus_tag="VW411_000136"
     CDS             complement(57905..58534)
                     /gene="upp"
                     /locus_tag="VW411_000136"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEU9792949.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLNLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(58623..59090)
                     /locus_tag="VW411_000137"
     CDS             complement(58623..59090)
                     /locus_tag="VW411_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEU9792950.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            complement(59109..59666)
                     /locus_tag="VW411_000138"
     CDS             complement(59109..59666)
                     /locus_tag="VW411_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU9792951.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            59791..60636
                     /locus_tag="VW411_000139"
     CDS             59791..60636
                     /locus_tag="VW411_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9792952.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            60695..61870
                     /locus_tag="VW411_000140"
     CDS             60695..61870
                     /locus_tag="VW411_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU9792953.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            61890..62345
                     /locus_tag="VW411_000141"
     CDS             61890..62345
                     /locus_tag="VW411_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9792954.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVNPDWSGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(62374..63114)
                     /locus_tag="VW411_000142"
     CDS             complement(62374..63114)
                     /locus_tag="VW411_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU9792955.1"
                     /translation="MYHIKEAAQLSGVSVKTLHHYDKIGLLVPLKSENGYRTYSQEDL
                     ERLQVILYYKYLGFSLEKIAELLKEERTDLLPHLTRQLDYLTRERQHLDTLISTLQKT
                     IQEQKGERKMTIEEKFTGFSYQDNQKYHQEAVEKYGQEVMGQALERQKGHEDEATAAF
                     NQVFQTLAQNLQVGLPATATENQEQAAKLLQAIRTYGFDCSIEVFGHIGKGYVYNPEF
                     KENIDKFGSETAQYTSDAIAVYVQTNAE"
     gene            63342..64689
                     /locus_tag="VW411_000143"
                     /pseudo
     CDS             63342..64689
                     /locus_tag="VW411_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466604.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            complement(64729..65673)
                     /gene="manA"
                     /locus_tag="VW411_000144"
     CDS             complement(64729..65673)
                     /gene="manA"
                     /locus_tag="VW411_000144"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEU9792956.1"
                     /translation="MSEPLFLQSVMQEKIWGGAKLRDEFGYDIPSEKIGEYWAISAHP
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDDLLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTVKADDLRSTLLVSNDFFAVYKWEITGKVDFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGSHFILPSDVEAWTLEGQGLELIVSHP"
     gene            complement(65794..66090)
                     /locus_tag="VW411_000145"
     CDS             complement(65794..66090)
                     /locus_tag="VW411_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEU9792957.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYQDGVDLLGYTTWG
                     CIDPVSAGTGEMNKRYGFIYVDRDNVGNGALKRSKKKSFYWYKDVIYSNGASIG"
BASE COUNT        19383 a        14454 c        12109 g        20175 t
ORIGIN      
        1 atctccagat taggaactat ccgtgagttc tctagtctgg agatttttca atagacttcg
       61 ttattggacg gttaccaagt tactcaaagt acagatttga agctgcagat aagactggcg
      121 aaatcacttt aatactaaat gaaaatcaaa aagcaaacta agaagctagc ctcaggctgt
      181 acttgagtac ggtaaggcga cgctgacgtg atttgaaaag attttcgaag agtataaaac
      241 actgttttga ggtagtggat aaatcggctg ggcaaaaact caatctcagg agaaaatgaa
      301 gtaaatctcc ccacaactat agaaaaagat ttgattctgc tcctcacaca ataaaatctt
      361 tttcttttat taaaaacgaa tcgtttcacg tgttttctca tatgaggtaa caaagcgatt
      421 ggttacacca ggctctgcca cttccaatgc ttgagaaatc ttgtcaaatg aagcgtgata
      481 atcaaattct ttttcaaaaa tatctaaagc ttcgttaaat gcttcttgaa tgcgttcatc
      541 aaatgagcga tagcggttag aatattgcaa gagttgctct gtcaaagttg catattgtac
      601 aatattataa gtttccgttt ctaaagcttc catatcattc gttgcaattt caagaactcg
      661 ggtaacagat tcaatgttaa tcattttttg ttctaactca accattaaat cctcggtatt
      721 attgcttgcc gtaaagaata acttcaagaa agtttgtgga atacctggca gattgcgttt
      781 ttccatgtat cgcttgatag tatggagacg attgacataa acattggcct tttgacgtgc
      841 attaatatca tctttctcaa tttgtgtcag gcgctcacta actgaaattt gctcatcttc
      901 aatatctttt agttgagttt gtaaatcctc aagattttct tcaagaactg aataagcttg
      961 ggttggttct tcttgatttg aagttacctc aacaatagct gcctcaaaac tctctaattc
     1021 tgtctgaata cgacgaacat ggcttgcagc tgtctcagga agtaaatagg tcttgttcaa
     1081 acgtgcaata tcttctccca ataaagtatt attctctttc atatgttgaa gataagttgg
     1141 aagagttgca agtagatttt ccactacttt ctgagcagca atttctcgag taaaaatatc
     1201 atacaaggca ttgatttcct cttgtgcctg tccattctca tattcggcat tatccaattc
     1261 caactgacga atattctctt ggtttttctt gaatgcttca taaagcaagt ggaaacgcgc
     1321 ttcaatatcc gtttcaacaa aatgataatt agcatcaatt agtttacgat aaccggcttc
     1381 caaatcctgt aattgatctg gcaattctgt agaaagcgtc gtaaccaagg ctggaacacg
     1441 atccacaata tgacttaagg ccaaaatgtg attttctgta ttatccaaaa tcactgcggc
     1501 ttccacaggg tcacccgatg aattcaaggt tacaaattgt gaaaattcag attggatatt
     1561 ttctaattgt ttttcaattt catccaaggc ttgaccatac tgttctgaat tttcagcaac
     1621 tctatgctga agttcctcaa ataaatccaa agcatgaaga acacgaccac tatttttaga
     1681 ttcttgcttc tctaagtctg ccaaagcatt gcgaattgcc gcaatatctt cttcaatcaa
     1741 agtaatttga ctctcaattt ggtcaatttg atgactggcc ttgagaaaac gaaatgaatg
     1801 gttatagcct tctgcttcaa agagattatt ttcaatatcg gcaaaagagt tgagagataa
     1861 atcgacccat ttttgattcc attcacggaa agccacttga ctttgtccaa tcaagtgcat
     1921 attttttaca gcttctactt catcatttac tggaagattg tatagttctt cttttttttc
     1981 ttctagcgcc tctaatctcc cctcgtttcg cttacgtaga aagattgcca ctacataagc
     2041 cagaactaaa atgactgcaa ttgcaaccat taaataaatt agttgtccat tagacatatc
     2101 aaactccttt tttacttgaa acaatcgtaa cgattatatc atatttttta gaccaaggga
     2161 actacttctc cctatttttt agacatcaag tgtactatag acagcatttt cttcgataaa
     2221 ctcacgacga ggctctactc gatcccccat caacatatca aagattttat ctgcttctgc
     2281 agcatcatct acagaaactc tagccatcaa gcgatgttcg ggatccatgg ttgtttccca
     2341 cagctgatga tcgtccattt cacctagccc cttataacgc tgaatagtcg gtttggtacg
     2401 accttcacta taacgggcta aagcttcttg gagtttgatt tcttgatctg cacccggctg
     2461 gatatattct ttaatctcgc ttccaacctt gacaccatag attggtggtt gggcaatata
     2521 aacataacca gcttctagga ttggtttcat ataacgataa atcaaggtta aaagaagggt
     2581 acgaatgtgg gctccatcga catcggcatc ggtcatcaaa acgagttttt ggtaacgggc
     2641 tttcgaaaca tcaaattctg cgccaaatcc tgttcccatg gctgtgaaaa gactacgaat
     2701 ttcttcgttg gctagaatct tatccatact tgctttttca acgttcaaaa tcttaccgcg
     2761 aattggaagg atagcctgaa actcacggtt acgaccagat ttggctgatc caccagctga
     2821 gtctccttcg acgatgaaga gttctgtttc agcagggtta ttagaagaac agtctgctag
     2881 tttccctgga aggttggaaa tttccaaacc agatttttta cgtgtgactt cacgcgcacg
     2941 cttggcagcc acacgagcct tggcagccaa aattcctttt tctacgatac gtttggcaat
     3001 ctgtggattt tccatgagga aatcggagaa agcttcactg aagaggcgat tggtaatctt
     3061 gaccacttcg ctatttccca atttggtctt ggtttgtcct tcaaactgtg gatttgggtg
     3121 tttaactgag ataactgcag ttaagccttc gcgaacatct tcccctgtta aattatcttc
     3181 attgtctttc agtaacttat ttttacgagc ataatcgttg ataacacgtg tcaaggctgt
     3241 acggaaacct tgttcatgtg ttccaccttc atgggtatga atattattgg cgaaactcat
     3301 gacattttca tggtaaccag ttgtgtactg catggctacc tcaactgtga tatcatccat
     3361 ctcaccgtct gtatagattg gtgtatcaaa gattacatcc ttgttctcgt tgatatattc
     3421 aacgtaacta gcaatcccac cttcataatg ataatgcttg gtttgttcca aaccttggcg
     3481 cttatctgta attgaaattt gaagaccgcg atttagaaag gccaactctt gaatccgttt
     3541 atttaattta tcaaaatcaa agattgttgt ttcagtgaag atttttgggt ccggtgtgaa
     3601 gtgaacagtt gttcctgttt tatccgtatc tccaactatt tcaagatctg cgacaacatg
     3661 accacgacgg tattcttggt aatgaatctt accatttttg tgaacatgaa cgtctaattg
     3721 agtggaaagg gcattaacta ctgacgaccc caccccgtga agaccacctg aaaccttgta
     3781 tccaccaccg ccgaactttc ctccagcgtg aaggactgta aagacggtct caacagcagg
     3841 acggcctgtt ttttcctgaa tatcgactgg gataccacgc ccatcatcca caacagtaat
     3901 cgaatcatct ggctcaataa aaacttgaat atggctggca aatcctgcca aggcctcgtc
     3961 aattgagtta tcaacaattt cccagactag atggtgaaga ccttcttttg aggttgatcc
     4021 aatgtacatc cctggacgca tacgaacagc ctctaagccc tctaaaactt gaatttgact
     4081 ggcatcataa tcctgtgcct gcagattttt gatttcttct gtcatcttat tcctttttct
     4141 tatatgtcta atacttgtct taaattcacg atactggtaa acaagtgggt atcaagtcct
     4201 gcagcttgac ctgcttcgat atcaatcggc cgatcaccaa tgacaagacc agagctaatc
     4261 tgatactttt ctcttaaata aagcatggat tcgggatttg gctttctctt aaagcctgag
     4321 ctagaagtca ccacttctgt aaaataagct gctatagagg ttttttctaa aatttccaaa
     4381 acctgatcat ttcgatgaga gaccaaaaaa tgacggccac cttgatttga aatgtcttcc
     4441 aataggtcag aaactccttc aaataaaatc gggtgttcaa gctctctggc ttcattttcc
     4501 ttgtactttt ctaaaaaatt ctctaaattg ggagcgaatg tctcaatcgc aaaaggagta
     4561 gaaaccttta aagcttgata gacactgtca tggtcttgtg tgataccata cagtgccaat
     4621 gtttcaacaa atgcagctgt tgaagtttca taattatcca gtaaagttcc acctaaatcc
     4681 cagatgtaat cgtgatattt catacccttc attatagcat ttttttatga aaaaagcgat
     4741 gatttccctg agttttccac ataaaaaacg agagaataac tcccgtttta ctgattttta
     4801 ccaaagacat ctcgatagag cattcctgtt cgtaaactct ctgcgtcccc tcctagttgc
     4861 tccaccaact cgtaggcaaa ggcaagggct gttgaaggac cccgactggt tgtcaactga
     4921 ccatctacca ctactgtttc cttgacgtag tgaccatcaa ggatttgctc ttgaacgccg
     4981 tcataacaag tgtatcgctt atttttcaat atctctgctt gattgagggc aattggtgcc
     5041 gcacaaatgg ctgctagttt cttcccttct tgctcgaagc tttgcaattc ttgaatcaag
     5101 gtctgattat cacgtaaatg tgcagaacca ggcatacctc caggaagaac aatcatatca
     5161 tagtctgata aatctccatc aaagacatga tctgctctta cttggattgc atgcgaaccc
     5221 gttacttgct cttcaaaacc aaccatatca catgtgatat tggctcgacg caagacatct
     5281 acaactgtca aggcttcaat ttcttcaaag ccctgagcta atataactgc tacttttacc
     5341 atgattttct ccttatttaa tttgttttaa tacaaccttt ttccgtttga atgggacttt
     5401 tccgctcttt tcttcagcta gattagtctg gtaactcatc gataaaagca aaccaacacc
     5461 aatcagatta ctgataatag ctgatccccc ttgcgaaatg aaaggcaagg gaatccccgt
     5521 caaaggaagt agtccagtca cagcaccgat attctcaaag atgtggaaga gcaacatcat
     5581 aatcaaacct gtggaaatat aagtgtagaa ctggttattt gatttaagag taatcttcaa
     5641 catacggtaa atcaacatga gatagagggc aataaccagg acagagccaa taaagccaaa
     5701 atcttctgca ataaccgtaa aaatcatatc tgactctcga actgggataa gcagattcga
     5761 agcattaaat ccctgaccaa ataagccacc actcccaatg gcaatctgcc cttgagcctg
     5821 ctggtaagtc gttgtttggg caaactcaaa gggattgagc caagccaaaa tccgattaat
     5881 ttggtaggtc ggcattccaa tctggtgaag aaaagctcgt ccgtccttgc taataaagat
     5941 agctaagaaa ccagcaactc ctgttacagc agtcacaaat actgggataa taattttcca
     6001 agaaacccct gataataaaa cgattcctga gaaaatggct acaaaaacca aagccgtccc
     6061 caagtcactt tgaagtgcta aaagaactag gactggaatg gtaaagagaa tcatccagaa
     6121 aattaacaaa aagtccagcg gaaccgtgcg tctccattcc ttatgtttct ttgtaaattg
     6181 gacaatgaca cgagccaaca tgaggatata ggatatcttc ataaattctg acggttggaa
     6241 tagggtaatt ccatttattg atacccagtt tttggcaccc gttgatgcaa ctaagcttgg
     6301 attataaaat acaatcggca agatcataag tcccaagcct aaaatatata gaaagggggt
     6361 caccttccaa agaaattctg tattaaagag catgaccaca aaaccaatca caagccccaa
     6421 ggcaatccag gcgacctgct gccctaaaat gggcagaata ttattgggat aatcatgact
     6481 aacggctata tagatagcca ccacaccgat gaccagtaga aaaaatactg gcaagagcaa
     6541 actgtaatcg actcttgagt cgagagaacg tttcatatat actaacctta tactttcata
     6601 caatactatt tatcaaagct cattaaaaaa tctatcaata gcctcgtcaa cttcggatcg
     6661 agagatggtt ttaacagtcg cttcttctgc tagagatgct actatttgtt tgccgtatcg
     6721 ttttccgacg attctcctat ccaaaataag agttaaggaa cgttggtatt cacgtctcat
     6781 acttcttccc aaagcctgtt ttaaacgaat aatggccatt ggcaattgat aatcataaaa
     6841 ggcatttttc ccttcttgat tcagttcttg attaatcttt ttcgtcaagg gttcttgagg
     6901 attttggaaa ggaagcctcg gtacaacttg aatcacagaa ggatggcttg aaaaatcaac
     6961 tccctcccag aaacttgctg caccaagcaa gatttgttgt tcaccttttt caaagcgttt
     7021 ctttagctga tgaacatccc catttttata ctgggccaag tggctaactg taagtaaatc
     7081 cgatactgct agaagcatgt ctttagcggt aaagagaacc aaaatcggtt gctggaaagc
     7141 ttgaatttcc actagtaaag cagctacctc tctggcatag acttctaagg aggtttctgt
     7201 taccagaggg aaatctttga ccatgaccac ttcttgttcc tgttttcccc gagattcaat
     7261 cttgacaaat ttagcttcag gatagcctaa aaggtctgcc aaagaaaccc tctgactaat
     7321 ctcaagagta gccgatactc ccaagacttg gcaactctca ggaagtaaag aggacagaag
     7381 agtacggcct gattttgtag aagaaatctg aattttcttt tgactcgttt cagttacttc
     7441 aagccagtaa tcaccttcag ctgtaaaata gcgaaccagc tcatcaaagt cttctacttc
     7501 caattctgaa aaatactgat ggagattgtc cagagaatct aagatatttt tcctagattt
     7561 gccagactga aattgttcta tcaagtagag acactcaaag cgaatacttt ctagtatccg
     7621 ttgttgaacc ctgttttctt ctcctactaa agccttatca attaaatcga taatagattg
     7681 tatatcgtag gtctcttgaa gcagattttc tagagctaac aaaatctttt ggacttcatc
     7741 aataatcagt aaacggtcac tgacaaattc aggattatct tcaagtctgg ttacgagata
     7801 ggcatgatta gtcactaaaa gcttgcaagt ctctgccctt tcttgactac gtttccaaaa
     7861 atcttccgtc acaaataagc tctgggatga taaattccca tcatgacgaa ggtctgctag
     7921 aaaatgttgg taacggtaga gttgcccgat ttcatccaaa tctcctgtct ctgtctcagt
     7981 aagccagacc aagacttgca ttttaaagcg tctaaataag cgattttcat cattttcctg
     8041 caaggaatga taaaaggcat ccaacttcag ataattttgt ggtcccttta agctatgaat
     8101 atctgtatgg aacacttcct tgaggcgttt accttcttct tccatgattt gattttgaag
     8161 aatctttgtc ggaacactaa gaacaatttg tcgctctttg gattgagata aagcgggtaa
     8221 gagatagcca taggttttcc caatccctgt cggcgcttga atcagagaga caggttcatc
     8281 tttcaatagc aagccaacct ctttagcaaa actttcttgt tcctccctca cttcaaggtt
     8341 caacagagaa atatttttag aaaagtcttg agatagtttt cgtggctcca gagaagcttc
     8401 cgttttctta aaatatagac cttgaacttg gaccaagtct ggagaactca ggatagattg
     8461 gttgcgataa gtttcctcaa taaccaggta ggactcatat aggagagcgt cagccatttc
     8521 cagcaagcgt tccaagagac ctttaggaag ctgggtcatc tttttccgta aaaaaagaag
     8581 taattctgct gtagcttggg catctgaaag ggctgtgtgt gcgtgtttaa gaggaattcc
     8641 taattctcga cacaaaatcg gcaagctata tttttccagt tcagggaaaa agacctgggc
     8701 caattcgacc gtatcaacac gagggtttct tagctcatag ccttcaaaaa ataaattttc
     8761 cgccaagaga ttagcatcaa actgaacatt atgggctaca aaaatcccat cctccaccaa
     8821 gtcaaatatt tttctggcaa cttgcgaaaa atcaggtgct tgcgccagac gttggtctgt
     8881 caatcctgtc agttctttga tatgagcatc caagggttca tgtggattga catccgtcgt
     8941 atagtgatcg acgatttctc cgtcctcaat cacgacaatt cccacttgga taattttagc
     9001 cttactacct gtgctagttg cctctaaatc caccacaaca tagcgattac caatcgtttt
     9061 cattataaaa aattatacca aaaaaagggc catttggcac ctgcaaacat aggataattt
     9121 tcaaactcaa gaacactact tttcttaaaa atcccatcga atagagcatt ctagcctgct
     9181 gatttttgta aaatagtgaa gaaatacgaa aaattgagag aagaaatgtt tgcatcttcc
     9241 ttctctcaac gaatcatgta ttttatctaa ccatgtcagg atcgtagtca taaaatcctg
     9301 tatcaaggaa acggttttta gggtgaagtt tacgaacttc ttcatccagc aagaaggctt
     9361 gagcatgact aaagttgccc tcttgaatca agctacgcgc ttggataaag agttttgcaa
     9421 tctcaacaaa gttctgacca ggagtgtaga gcccttctag tttccagcga gtaaagccat
     9481 gctccaccaa ttctgttaat ttgatcatca aatcaaggtc attgttggca aagatatggg
     9541 tcccatgatt atcttcaaaa atggaatagt ggctctctgg atcacttggc tcagccaaga
     9601 agaggtcacg tttatgcgtc ttttcatcat cgatatgtgt aaagttatag tagttttgca
     9661 agagtggacg tttagaatga tggatgacgc tagcaccgta aaccaaaact tcagcaggaa
     9721 tttccaaaat ctctggcatt ttgaaaagtt cagctgatgg aatttcacgc gccaaaacag
     9781 cctcagatgc gccagccttt tgtccccaga agttaatctg acgactgcta gttaccatgg
     9841 ttgaagcatc gtagatggtc ttaaatgaat aaccatcgcg gttaactacg taaaagacgc
     9901 ctgcatcccc aatcgtaata tagtctgtct tgatttcttc caagaagttt aagaaaggct
     9961 tgatacggtc catcatatct tggtgcatga gagcattgac cgcaacgatc aattccttac
    10021 cagcatcatg aaccaactta gcgatttcac gtaattggtc ataactaaag gtcgttggca
    10081 gacgaagacc aaaatctttc tcaccgacat agatacggtc tacgccagct tcgagtagtt
    10141 gttcaacttg ttcaatactt tcagcagttg ctgtaatgac aatcttttcc ataagaaaaa
    10201 ttataccaca tttctccaaa atcagccatg ctttgaaaat gaaaagggac tttattcttt
    10261 ttgtaacctt tctgtaataa ttctctgccg aaaaaatgat aaaataggta tgtactgtta
    10321 aggagagaat catgcccgta agaaaattac aatcctatga ggtagactat caagaagaat
    10381 taaaccagca gcttcctcat taccaagctt atacacctga agcgcaatct gatgccaatc
    10441 tcaaggaaat cctatttttt attaatatcg ctgttttttg tatctgtatt gctatcttta
    10501 gttttatctt tttagcatta aaattatcaa ctgctcttgc ctttgccgca gcaatcggat
    10561 tcagcttact tgttttaaaa gttcaacggt ctattatcaa acgaaaacgt agaagataaa
    10621 tccaaaatat cgccccaatt ggagcgatat ttttattttt tctttttaga tggtgtctga
    10681 atggcaatct tcacttcaaa gattgttccc ttgggtttat tatctttgac agtaacagtt
    10741 ccttttagag catctacaat ttgcttggct agggataatc ctaaaccaaa accacctttt
    10801 tgccgggttc tagccttgtc tactcgataa aaacggtcaa aaattttctt tttatcttct
    10861 gtcgaaatac cgattccatt atcagaaaca agtaaataaa gattgcgatc ggtcgccgag
    10921 ataagaaaat caatttcacc atcctcctca gtatacttga cggcattatc gaaaagaatg
    10981 gtcatcagtt gtttcagaag aagctgatct gtgacaattg ttcgatggat acgattttca
    11041 aaacggaaga cacgattatt ttccgaagca atcatctcgt agtttgtgaa agttgtatta
    11101 aaaaagctag ttggaacttc tgcaagctcc ggcttaatcc catcatctct ccgagctaag
    11161 ttcagcaagc ttgtcgttaa aaaacgcata tttcggactt cttccaaact cgatgcaatg
    11221 ctttcgctca catccataat ggtagcttct ggcttacgaa aaagggtctc taagcgattt
    11281 tgcaaaactg cgagtggagt tcgtaactca tgactggcat tttccacaaa agactgttgc
    11341 ttctgcatac tctcaagcag gggcctaaca ctgaccctag ctagatagag actggcaagt
    11401 aaagacaaaa tccagaaact agccatcacg accacaatca attgctcatg cttttgacta
    11461 gcctgttcca actgactggt attaatcaag acagcagcat acttgatatt ggttgaaacc
    11521 gaactaatat tggtctccat caaaatcaca cgatagattt cttcctgccc atagctatta
    11581 aaaacctgaa tctggtagat atgtcctagt tctttctttt ctaacttaat cttatccaag
    11641 cccaaaaatc gatttccaga aagaagttga gtaaagtctt tatcaaagag aatgacttcc
    11701 gtattggaac tgacattagg ttttatttct actttactag cgtccgccct agcattttct
    11761 aaatctttaa tctcttctgt tgccctattt atagccagct gaataactgc ttgaggattt
    11821 tcactcagtc catgaagctt atcgtccacc gaagtataaa gactcgaatg catgacttgt
    11881 aaaataatca gagtcattgt agaaaaaatc agggtgaaga caccgaagtt gcggataaaa
    11941 taactaaagt catccgcata ccatgttttt ttaagtttac tgaacatctt ttaaaagata
    12001 cccaacacta cgcaaagttt gcaaattctc tgcaaaagtg gttcccttta atttcttacg
    12061 gacttttgaa acatagactt cgacaaccga aatcgttgta tcactatcaa atccccatag
    12121 acggtcaaaa atctgcgtct taggcaaaat cacattttga ttttgaagga aataaactag
    12181 taaatcgaac tctttcccca gcaattcgac aggagtatct tcaactttaa cggtattggt
    12241 tgataaatta accacgatat tcccataagt caaggtgttt tcattaaact tccctgaacg
    12301 tttgagaagg gcctgaatcc gcattttaag ttcttctagg tagaaaggtt tggtcagata
    12361 atcatccgct cccagttcaa atccatgtcc cttgtcatcc aaactttcct tggcagtcat
    12421 aatcagaact ggtgtcgtaa ttcccttttc acgcaattct tttaagactt ggaaaccatt
    12481 tttttctggc aacatcaaat ccagcaaaat caagtcatag acaccactct cagcttcgta
    12541 gagaccttct tctccatcaa atacctgcat aacatccgca aaatcgtcta aaaagtcaaa
    12601 tactgaattt gacagaccta ggtcatcctc aaccaataag atttttatca tgagaaactc
    12661 ctccttatta aaactattat accaaatttg ccttaaaaaa aactcaactc tctgcatttt
    12721 acatgagata gctgagtttt ctttttattt taggcttatt tatgcatttc cgtattgaag
    12781 aacaactgct tcgactgcag ctttttcacg gctaatcaag tcaacacgcg ctgcaatttc
    12841 cttgattccc ataccgatgt tacggctaag agcaaggtca gaaagttgcg gttcaaagaa
    12901 ctccttgtat tccgccaagc gttgctgagt cttaaataca tgagcaggaa ggataacaaa
    12961 gctatcaaag ctcatatctc ctccaagggc tgccttaatc caagcccagt tttcacgcgc
    13021 ccaagaccaa gctgttttct gagttgcttg atgagctagg aattggtaat accaagcaga
    13081 caagtcctgt ggtttgacca caaatttgtc cttccaagaa gtaatcaggt tttggatatt
    13141 atccgcatct gtactgtatg caagagctgc tgccaactgg cgtttaaaga cagcatctgt
    13201 tgcgtgagta taagtatcaa gataaagtgc taacaagtct ttagtctcat gatgtttcat
    13261 ctcattaatc agaacttgtg agcgaatagc tgctgggagt cctgcaagat tctccttgtg
    13321 tgttgcgaag atttggctag cgacttgact agcttctgca tcatttgagc gaatcatcat
    13381 cgaaacagcc agctgacgaa ccaattcatc ctcatctgat tctccgtctt tagcttcaaa
    13441 accaagacgg tcatagttat gacgagccaa tttagcaacc agtcctttga aggctgtttc
    13501 agcatccgtt ccttcatcaa taaagcgctc aagggctgaa atcacttgag aaacagctga
    13561 aaccaccaga taagactctt ccttagcaag tttatcaaga actggaagca agtctgcata
    13621 agaaatgtgc cctgcctcag ccaacaaacg acgttcttga acaatttgca gtttgcttgt
    13681 gttatcaagt gtctctagct cagcaagaac agctgctaac aagtctcctt gatagtcggt
    13741 aatatagtgg gcagtatttt cagtgttgag acgaagagct ccttcatttt cagcaagaag
    13801 agctgcgtag ccagggattt cgatactttc agtttcgagt gtatcaggca agcctttcca
    13861 gttgctattg aggggcacca cccagagacg gttcttgtct tcgttctcac cgatgaagaa
    13921 ttgtttttgt gaaatcttca agacatcatt ttcaacttta acagtaagaa ctgggtaacc
    13981 aggctgttcc aaccaagaat ccatgaaggc tgcgacatca cgtcctgacg cttgaccaag
    14041 ggcatcccaa aggtcactac caatggtgtt gctgtattgg tgtttttcaa agtaggcgtg
    14101 caaaccttta gcaaaatcag catctcctag ccaacggcga agcatgtgca tgagacggct
    14161 tcctttggca tagacgatag cgccgtcaaa gagtgtattg atttcatctg gatgtttaac
    14221 ttcgacgtgg acagactgaa cgccatcagt agcgtcacgt tcaagagcaa gaggtactcc
    14281 acctgtttgg aaatcttcaa agatattcca gcttggttcg atggtatcca cacagacgta
    14341 ttccatcata ttagcgaaac tttcattgag ccaaaggtca tcccaccatt tcatagtcac
    14401 gaggttccca aaccattggt gagccaattc atgggccaca acaagggcaa cttgttgacg
    14461 gctagcaaat gtagagttct catcgacaac caagtaaact tcacggtagg tcacaagacc
    14521 ccagttttcc atagcaccag ctgagaagtc aggaagggcg atgtggagag attgaggaat
    14581 tgggtactta actccatagt aatcttcgta aaactcgata gagcgaacag cgatatccag
    14641 tgagaaatca agatttgaaa gtggatgtgc tttggttgag tagacaccta ccagggtacc
    14701 atttttagtt ttagcggtca ccccttgcaa atcaccagca acaaaggcca acaagtaaga
    14761 agacatgcga ggtgttgtct caaacttcca gatacctgtt tccttacggt tttcaacatc
    14821 gatttctggc atgtttgaca aggccaattc accttctgct tggtcaaagc gaagagagag
    14881 gtcaaaagtt gctttggctt caggctcatc cacacatggg aaagcttcgc gcgcaaaatg
    14941 gctctcgaac tgagtagaca agacctcctt cttgactcca tcaactgtat aataagaagg
    15001 gtaaatccct gtcatgttgt ctgtaatttt accagaaaag gcaagaacca attcaacttg
    15061 accagcctca gccaattcga tatgaagggc ttcattgtca tggtcaactg taaatggacg
    15121 agcttgacct gcaacttcta cagaggtgat ttccaaatct ttttggtgga gggagatgcg
    15181 gtcactctgt gcttgaccag tgatggtcac tttcccagaa aaagtcttgg tctcacgact
    15241 caaatctaaa aataaatcat aatgttcagg aacaaattgc ttaataaaat gttcaactgc
    15301 ttgcatagtt ttctcctatt ctaagtttaa gagctgctgc tcttcttcaa acaaacttat
    15361 tatatcatat tttgcttcag aaaaaaggat tggacagcga tttccaaaac tttcttcctt
    15421 ctaatagtct acagtgggta gaccttgcga aattcttcta aaacaacctt gctttcaggt
    15481 gtaaaagtca atcctgcttc acccaagcag tctctgaaaa aatcctcatg aacctggcgc
    15541 caatagacaa gtgtcttgtt tccctcacct tccttatagg catggtcagc tgacactcga
    15601 ttgaagggtt taacagaaac ctttgtaatt tcgacaatgc agacagcctg attttgacta
    15661 tctaaaatga catcgaaggt ccctacttgg ggaagtggtt cgtcttctag cacatagagg
    15721 tcataggctg atgctgttgc tgtcttttct cctttaaaca ccaaatccgc taaaaggtct
    15781 ggttcaactc caaaagccca ggcatcgatt tcatctccga tcaaaggatt gatttgcttg
    15841 tatttattcc acatttcttg cggtatcatg ggtgctcctt tgtaattttt tactttcttc
    15901 ttttatgtgt ttaagatgat ctggatggtc aatctctaaa tcaaaaatct ctggaataga
    15961 actgtagtgg ataatgcact tgatacccaa ctgattcatt ttttgtatga aagaagtatt
    16021 cagatagcct gctacagcaa aatcaatctt gttctttctt gctttatcct gcatatctct
    16081 tagcatatct aacattattg gactttccat atcatgccat tgactgtttc tcatagtcgc
    16141 aaaaacaaag gaagtcaaat cattcattcc aactacaatc tttgaaatgc ccgtttccag
    16201 tatactagat aagtcaaaat acgctgacgg taattcaatc atcgttccga ctttcccagt
    16261 aaaaccctgc tgacgcaata ctgtaatagc ttgttttaat tggtcggcat cattgacaaa
    16321 aggaaagata acagatagat tggggttggt ttgataaact tctgtaacga catgtgcttc
    16381 agcctgaaat tcatccaaac acgccagtaa acgcctagtt cctctatagc caaacaaggg
    16441 atgcccttcg tcaaaaaact ctttagtccc cactaaacaa ttggcttctg tattcgttaa
    16501 ttcagtaaaa cgataccaaa cttccttacc taagtaaaag gagcaaatag tatcaagata
    16561 atctttcaca aattcctgac aactttgtaa tagtatattt tgattgagct ctctcaataa
    16621 gtattcccca cgaatcatgc cgacgtggtg aaatagttga ggataaattt tttcaagaat
    16681 tttttcgcca ctaagggcaa gttgatttct catcattcac cttccaattc atgtaagaag
    16741 tcttgtccag ttctggaaat cctaataatt cagacttaac cttcaagact aatggcgatg
    16801 cattttcttc tgtaatctct tgaatatcca tccaaatata tccaagtgaa tcattcgcac
    16861 catcagacac agcttccgaa atcgtaactt gaggtgcact ctcattcatt tcaacatcat
    16921 acaaggctat gacatggtga accataaaat tttttaactc ttccctgacg aaaacatcgt
    16981 agattcgagg attagagtag cttctaacag taaatcccgt ctcttccata acttctctag
    17041 tcagcgtttc cgtcagtcct tcaccaagtt gctgactgcc tccaggtaga tcataccgat
    17101 gttgataagg gcctctcgtt ttttcaatgc aaagtaactt tccattttca aagcaaacac
    17161 agtagacccc aaagtgattt ttgatttcca tccaactcct cctacttcaa agaccagcca
    17221 ccatctattg tcaagatttg tccttgcatg gcgctcgctt ttccacttgc taaaaaaaga
    17281 ctaagctctg ctatttcctc tggctcaatc cagcgcttga ttggggtttc actagccacc
    17341 cagtcagcca aaccacctgg ttcaaaatcc gcagcggtca tagctgtctt gactgctcct
    17401 ggagcgatac caaagacctg aatcccagct tcagcatagt ctagagccaa ctgcttggtg
    17461 aagccagcca aggcatgctt ggatgaagta taggcgtgac cacctccacc tgctaggcta
    17521 gaagcaatgg aacacatatt gatgatgatt ccctttttat tttccagcat ttgtgtcaaa
    17581 taataccgag tcaactctac tggaataatg tagttgattt caaaaatctc ttgaatgtcc
    17641 tgcgccgttt gttccaacag tggtttgtaa tcatccaaaa ctccagcagt attacacaaa
    17701 acatccacct gagggcacca gtcaaaaata ggttccaagt ccaaggtcaa atctctctgt
    17761 aaaaagcgaa aatcaccctc taagagtggc ttttcacctt ggtcaactcc ataaacttga
    17821 tagcccttct ctaaaaagag gcgagcttga gccaatccga tccctgaact cactcctgta
    17881 atgagtacac gtttagtcat gcacttctac ccaatccgtt gccaaaacat cacaaactgt
    17941 cgggctccac atggaaaaac cttctccttc gccagaaacg ttgattagga aataaggtgt
    18001 catttcaagt gcaagcccat tttgctcgat ggtatcaaag agttggacat agttttccgc
    18061 acctccccaa ccagttcgta catattttct cttagccttt aacccaggca ggatctcttc
    18121 aaatgtcatg tttttctcct ttaattctac attcttcatt taattatagc aaaaaaccgc
    18181 tttatacggc tttttgaatg tgagttattc aaacctgcta ctacttacgg caaattattc
    18241 cctgcagcaa gataaatttc ataccattct tttcttgtta agctaaagtt tgccgctcgg
    18301 ctaacttctc tcaagtgctt aggatttgtt gtacctacga ctgcctgcat ttttgctgga
    18361 taacgcaata tccaagaaat ggcaatagtt gaagaggtta ctccatattt aatagctaaa
    18421 cgatcaagta cttgatttaa agcttgaaat ttctcatttc caacaaaatt ccctttaaaa
    18481 tacccgaatt gtaagacaga ccatgcttga atgaccacat cgtgtaattg gcaatattca
    18541 aaaatgctgc catctcgcat agctgcttga ctatcttcca tattaacatg aaaagctgat
    18601 tcaaatcctg gagtaaaagc cgcactcaat tgtagctgat taacagctaa cggctgcttg
    18661 acatcttttt taagcaactc catcatcata ggattttgat tagaaactcc aaaatctcga
    18721 actttacctt gtttataaag gagattaaag gcttctgcta cttggtcaga ttccatcaaa
    18781 gcatctggtc gatgaaggag caagctatct agatgatcaa tcttcaatct ttgcaaaata
    18841 ccgtctactg attttataat atagtcctta gaaaaatcaa aataggtaaa ttcttcaatg
    18901 cgaatgccac atttggactg aatccacatc ttttctctta aatctggacg attttttagg
    18961 acaagaccta acagttcttc acaacgacca cgaccataaa tatcagccaa gtcgaaggca
    19021 ttgattccaa cagaaagtgc tgtttctaca agctcttcaa cttcttttac agatttatct
    19081 tttattctca tcattccgag aacaatttct gataattctt tgtcatcttg accaagagtt
    19141 atgtatctca tcaaattttt ctcctttaat ttctaacatt cttcccttca ttataacaaa
    19201 aaaccgcttt gcaacgactt tttgactata cttcactcca ttttatcttc ttaaacccac
    19261 ggaacaagac aaagattcca ataaagagga cagctaaagg aataactttt gtaaggaaaa
    19321 catttgaaat tcccatccac tcataataac ggagcagaga acccaccaca agatgggcaa
    19381 taatcatact gacaaatgga cgaaagaccg cttctttcca attccaaata ccgataacta
    19441 gcgaaatcgt aaagacagac aaactatccc agggagccgg aatataaaag gctccttctt
    19501 gtatgaagct tgccattcct acatatccta aaacaactag aagaactata gtcccaacaa
    19561 caatgtaagt gccaattttc attttaggag aatcttggac taaacttctt cgtaaaattg
    19621 tggccacaag tccaaatcca atcagaaaaa taagaagttg ccctaaaaat gtgagcaaat
    19681 tgactgttaa gagaggacct ttagaaaaat cacttagtag ttgataataa cgtaataccg
    19741 ccaggacaag aattggcgtc aaaagggact ctttgataga actgcgaggt gctcccttga
    19801 gaatctcttt cattattttt ttaggattct tacctagata atcctctgca ctcatgccat
    19861 ctcgttctgc ttctgagaaa tctagcatca tcaaatagat ctgctctctg agatagtctt
    19921 catcatagag aaatccagca agattaaaac tttcccacaa ctcctcaaaa tacttttgat
    19981 tctcctcaga aaactcatgt agcaaagcgc ttgtttcttc gtaatacttc attttcttca
    20041 tggtttaacc cccattctta atcccttcta ctttttgact caaatcgtcc cattgttgcc
    20101 aaaagactga gacacgctct tctccttctt tcattaatga aaaatacttc cgatctggac
    20161 catctggcga cgggcgcatg tcgcctctta tccattgatt tttttctaac ttttgcaaca
    20221 aaggataaat agttcctgga acgatagtat caaatccagc ctctcgcaaa gtctgaacca
    20281 actcataacc ataccgctct ttttgaccaa tcatatccaa gacacaacct tcaagaacac
    20341 cttttaatag ctgagtttct ttcatcactt ctcccttcta atctattttg taatacctac
    20401 tagtgacttc acctatagta tatcacttct acactagttt gtaaagcata atagttaata
    20461 ctcttcgaaa atctcttcaa accacgtcag cgtcgcccta ccgtatgtat ggttactgac
    20521 ttcgtcagtt tcatctacaa cctcaaaaac atgttttgag ctgacttcgt cagtttcatc
    20581 tacaacctca aaacagtgtt ttgagctgac ttcgtcagtt tcatctacaa cctcaaaaca
    20641 gtgttttgag ctgacttcgt cagtttcatc tacaacctca aaaacatgtt ttgagctgac
    20701 ttcgtcagtt tcgtctacaa cctcaaaaca gtgttttgag caacctgcgg ctagcttcct
    20761 agtttgctct ttgattttca ttgagtataa ataaaaaaac agaactagcc tgaactagtc
    20821 ctgtctactt ttacccaatc acacttccat ttggtacagc tggatcaact gtgagaaggg
    20881 ttaatttgcc atcatgttca gctgagagaa tcataccctg gctgacatat tttttcatca
    20941 ttttacgtgg tttgaggtta gcaacgattt gaactttctt gccgaccaat tcttgttcat
    21001 ttggatagta ttttgcaatt cctgaaagaa tctgacgatc ttctccatca ccagcatcca
    21061 agcggaattg aagcaactta tctgaacctt ctactttaga cacttctttg acttctgcga
    21121 cacggatttc aaccttgtca aagtcttcaa acttgatttc atccttgttt agtttgagct
    21181 caacttcgtc cggattccat tctttttcga ctgctggttt attgccttcc atttgttcct
    21241 tgatataggc gatttcttct tccatattta gacgtggaaa gataggtgtt cctttggcaa
    21301 ctacagtcac atctgctggg aagtcagcca aactcaagtt ttcaagacta gaaacttctt
    21361 ccaaaccaag ttgagtcaaa actgcacgac tagtttccat cataaatggt tcaatcaagt
    21421 gagcaactac acgaatgctg gctgccaagt ggctcatgac acttgccaat tggtcacgaa
    21481 gagcttcatc cttggccaag acccatggtg cggtctcatc gatgtattta ttggtacgag
    21541 agatcagagt ccagactgct tcaagcgcac gtggatagtc aactgcttcc atgtgtgtat
    21601 ggaagtctgc gattgattgt tctgcaacct cagcaagaac atgatcaaat tcagtcacac
    21661 cttctacata ggcagggatt tgtccatcaa agtacttatt aatcatggaa accgtacggt
    21721 taaggaggtt cccaaggtca ttagccaatt catagttgat acggccgaca tagtcttcag
    21781 gagtaaaggt tccgtctgaa ccaactggaa ggttacgcat gaggtagtaa cgaagtggat
    21841 ctagtccata acgctctacc aacatttcag ggtaaacgac attccctttt gacttagaca
    21901 tttttccgtc tttcatgaca aaccaaccat gggcaatcaa acgatcaggt aatttaacat
    21961 ccaacatcat aagaaggatt ggccagtaga tagagtggaa gcgaaggata tcttttccta
    22021 ccatatggaa gactgttcca ttccagaact tgtcaaagtt accatgttcg tcttgagcgt
    22081 agccaagagc tgtcgcatag ttaagaaggg catcaatcca aacgtagaca acgtgttttg
    22141 gatttgatgg gacaggcact ccccatgtaa aggttgtacg agataccgcc aaatcttcca
    22201 agcctggctc gatgaagttg cgtagcattt cattaaggcg accatctggc gtgataaatt
    22261 caggatgagc tttgaaaaat tcgaccaaac ggtcttggta tttgctaagg cgaaggaagt
    22321 atgattcttc agaaacccat tcaacctcat gacctgatgg agcaatacca ccagtcacat
    22381 ttccagcttc atcacggaaa acttctgcca gctggctttc tgtaaagaat tcttcgtctg
    22441 atactgaata ccaaccagag tattcaccca agtagatatc atcttgagca agtaagcgtt
    22501 caaagacttg tgcgacaact ttttcatggt agtcatcagt tgtacggata aatttatcgt
    22561 atgagatatc tagtaattgc cagagttctt taactccaac cgccattcca tcaacatagg
    22621 cttgaggtgt aataccagct tcttccgctt tctgctggat tttctgacca tgttcatcaa
    22681 gacctgtcag ataaaataca tcgtagccca tcaggcgttt gtaacgtgct aggacatcac
    22741 atgcgatagt tgtgtaggca gaaccgatat gaagtttccc agatggatag taaatcggcg
    22801 ttgtaatata aaaatttttt tcagacataa tttttccttt ccaggcaaat gaaacctgtt
    22861 tttctaacac ttcattatat cacattttta atgaatttca atagggaaat ccatacaaaa
    22921 acaagataga cgagtgtcca tcttgttgat ctcattcata acgaagggct tcaattggat
    22981 caagtttcga tgccttgttg gctggcaaga ctccaaaaat cataccaaca ctagccgaaa
    23041 ctgcaagact aaatagggcg actgggattg atactccaac ttctatacct tctattaaac
    23101 cttgcagtaa caaacctgct aaggcagtta aaccacttgc aattgtcaag ccaattaagc
    23161 cacctaacaa ggtcaaaatc atggattcaa tcaaaaactg aattaaaata ttggcacgtg
    23221 ttgcacccaa agccttacga agaccaatct cacgagtgcg ctctgtcacc gaaaccagca
    23281 tgatgttcat gacaccagtt cctccaacaa agagagaaat ccctgcgatg gaactaataa
    23341 tcgtcgtcat aaaactaaac gattgttgaa tttctgcaaa tacaacggac tcatctgcca
    23401 cctggtattc tccctgttgt aagcctgcaa gctctgtcat ttttcgtgcc agttctggac
    23461 ccagagttgg ggttaaactg gtatcattca ctcgaaagac aatattagct atttcatcta
    23521 cattaaaatt cgcagcaagg gagatattgg tagtaatagg caagccacca aacccatata
    23581 tttttgatct tttagcctcc ggactagtat aaaccccaat gacccggtaa ctaaatccat
    23641 tgacttctac aaccttgtta atagcctctt gaggagattc aaataaacta atggacaatt
    23701 cctcatctag caaaatgaca cttgcaaact ctttgaaatc ttgctctctc agactacgac
    23761 ctgcaataat ttcattctta acagcgtcca tgtaagttct gtttccacct gtcaaattag
    23821 cattctcaac ctttttatct tgataggtca agatggcatt cgttgaattg gttacatagt
    23881 aactatccac tcccttcagt ttagctgcct cttggaccca ggattcttgc ggttttggcg
    23941 gttcaacagg aacttcctct tcctttccag aaaccgtaaa agctgattgt ttctgagtaa
    24001 aagacccgtc tttacttttt ttaggagaga aaaagacgct aatatttttc tgagatttag
    24061 tcatatcttt attgacttga cgagataggg aatcacccaa agccataatc acaacaactg
    24121 atgaaacacc gataataatc ccaatcatag taagcaaaga acgcatcttg tgagccatga
    24181 tagatgaaaa ggcaaatttc agattctgca tcttagtttt cctcctttcc taactgagca
    24241 ctgtcagacg aaatgacccc atcccgaatg acaatctgac gtttggcata ggcagcaatc
    24301 tcaggctcat gcgttaccat gataatggtt tttccttctt tattcaaatc aaccaataat
    24361 tgcataattt ggttacctgt tttggtatcc aaggctcctg tcggttcatc cgctaggata
    24421 atagaaggat tgtttaccaa ggcacgcgca atggctacac gttgcttttg accaccagat
    24481 aattctgaag gtaaatggtg actacgttct gtcaattcaa ccttgtctaa atattcctca
    24541 gccaacttgc gacgttttga agacgaaact cctgcgtaaa tcaagggcaa ttctacattt
    24601 tgcagagcat tgagcttcga tagaagaaag aactgctgaa agacaaaacc gatttgttgg
    24661 ttacggacct tagctagttg tttttcacca agcccagcca cttcttgacc ttcaagataa
    24721 tattctccac tggttggtgt atccaacatg ccaatcgtat tcatcagagt ggacttacca
    24781 gacccagatg gtcccatgat ggctacaaat tcaccctcat tcacttctag attgatattt
    24841 ttgagaacct gcagttcttg gtcaccatta cggtaacttc tgaagatatt ttttagacta
    24901 attagttgct tcatcagcct tcacctcttt tccttcttcc aaggaagatg ttggattact
    24961 gatgacctta gcaccgttcg ttaaaccaga agtgatttct tgattttctg cgtcagcatt
    25021 tcccaatgaa acctcaactt ttttagcctt ttgttgttca tccacaatcc agacataatt
    25081 tttactatca tccattacta gactgctaac aggaacaaga atagccttag ttttgctttt
    25141 aacctcaatg ttgacagaaa aaccttgttt caaatcacca acctcgcctg tcacatcaat
    25201 agtataaggg tatttagaac ctgtattatt cccggctgct ggactagctg cttcaccatt
    25261 gtttttagga tagtcagaaa tatagcttaa tttcccagtc cattttttat caggatacac
    25321 tttagaagta aagcttactt cttgacctac agaaaggttg gctagattgt actcagacaa
    25381 ttctcccttg acttgtaaat tttcattgct gacaatatga accataactt gactcgcccc
    25441 tgttggagat ttagaaacat tgctattgac ttcgaccaca gttccctcta gggtactgag
    25501 aacagttgtt gcatccaatt gactttgagc cttgcttaat tgcgccgcag catctgcacg
    25561 cgcatcacgg gcatcaccca attgagcgtc aatagaagca acagaatttc cagccactgg
    25621 agttgggctt tgcaccgttg catcttctcc tcctactggc gctggtaact gtggagccgg
    25681 agctgaagcg gcttcatttc gtgcttgatt gagttcattg atatgacgat ctgccctagc
    25741 tactgctcga ctagctgaat cataggccgc ctgcgcttct gaactactgt acttgactaa
    25801 agcctgccct tcgctgacct tatcgcccac agaaacaagg atttcatcta aatcaccctt
    25861 actagcatca aaataaacat attgttcatt ttttgctgtt actgtccctg acaataaaac
    25921 agaggaggcc acgcttcctt ccttggcaac aacaagatga gtaggctcat cttttagagc
    25981 agtctgagaa ggttgtctaa agagtaaaat ccccccagca cccaatacaa ctacactcgc
    26041 agcaccgatt gctgcataca gttgccactt tttagcttta ccattctttt tcttcataat
    26101 gaaactcctt ttctttttta caatactttg ctattatacc aaatttccct ccagcaaaca
    26161 atacagttca ggattaaaca atcgttcgga attttgcttt tctattgtac tagatatata
    26221 atacactatc ttgacttatt tggcaagctt ttttgcaatt ttttgtgcgt agcagatttt
    26281 tgcaatcaaa tcaaaaaaag aataaaatat aactatcaaa aaagacgctc tactatttaa
    26341 aagattacaa aggagttttc aatgagagaa tatgatatca ttgctatcgg tggaggtagc
    26401 ggaggaatcg ctaccatgaa ccgtgctggt gaacatggag ccaaagcagc cgttattgag
    26461 gaaaagaaat taggtggaac ctgtgtcaac gtcggttgtg ttcctaaaaa aatcatgtgg
    26521 tacggggcgc aaatcgctga gactttccat caatttggag aagactacgg ctttaagact
    26581 actgatctta actttgactt tgcaacccta cgtcgcaatc gtgaagccta cattgatcgc
    26641 gctcgttctt cttatgatgg tagttttaaa cgcaacggtg tagacttgat tgaaggtcat
    26701 gctgaatttg tagattctca tactgtaagc gtaaatggtg aactgattcg tgctaaacat
    26761 atcgtgattg ctacaggtgc ccatccaagt attcctaata ttcctggtgc tgagctaggt
    26821 ggctcttctg atgatgtatt tgcctgggaa gaacttccag agtcaattgc cattctaggc
    26881 gctggttata tcgccgttga attagctggc gtactccaca cttttggtgt caagacagat
    26941 ctctttgttc gccgcgatcg tcctttacgt ggttttgatt cctacatcgt tgaaggtttg
    27001 gtcaaggaaa tggaaagaac aaacttacca cttcacactc acaaagtccc tgtcaagtta
    27061 gaaaaaacta ctgacggcat taccattcat ttcgaagatg gtactagtca cacagctagc
    27121 caagttatct gggctacagg tcgccgtcca aacgttaagg gcttgcaact tgaaaaagct
    27181 ggagtgactc tgaacgaacg tggctttatc caagtggatg aataccaaaa tactgttgtt
    27241 gagggaatct atgctctagg tgatgtaacg ggcgagaaag aactgactcc agttgcaatc
    27301 aaggccggac gtaccctatc tgaacgtctc tttaacggaa aaactactgc aaaaatggat
    27361 tactcaacta ttccaactgt tgtcttttca caccctgcta tcggaactgt tggtttgaca
    27421 gaagagcaag ctattaaaga atacggtcaa gaccaaatca aggtttataa atcaagcttt
    27481 gcatctatgt actctgcttg cactcgcaac cgtcaagaat cccgtttcaa actcataaca
    27541 gctggttcag aagaaaaagt tgtcggactt catggaattg gctacggcgt tgatgaaatg
    27601 attcagggat ttgccgttgc tatcaaaatg ggagcaacca aggctgactt tgatgcaact
    27661 gtggcaattc acccaactgc atctgaagaa tttgtaacca tgcgttaatc gaaacaaaag
    27721 aagctgatat aaatatgtct gcttcttttt taatgatttt cgaatcaaca aaaaaattat
    27781 ccaacttgac tagtcacgaa aatcggctgt tacagttttt gttaccttgt ttttaaaaaa
    27841 tggttgacat cgactcctat atgagtataa tagttactat acattagaaa agaggttaac
    27901 tattttgaaa aaagctcacg tttatgctat ccctgctatt ggggctgctc tcattgctgc
    27961 tttggcacaa atcagtcttc caattggacc tgttcccttc actctgcaaa actttgcaat
    28021 cggcttgatt gctactgtct ttagaccgag agaggctgta ctttctgctg gactctatct
    28081 tcttctaggt gctatcggtc ttcctgtctt tgcaggaggt ggagctggtt ttcaggcttt
    28141 agttggccct actgcaggct atctttggtt ttatctcgtt tactctggac ttacttcctc
    28201 tctaaccaac agcaagagtg gtgttgttaa gatttttctt gcaaacctct tgggtgatgc
    28261 ccttgtcttt gtcggcggga ttctcagctt gcatttccta gctggaatgg catttgaaaa
    28321 agctcttgct gtgggggttc ttccctttat cattccagac cttggcaaac ttctagctat
    28381 tagttttatt agccgtcccc tacttcaacg ccttaaaaat caggcttact ttactaacta
    28441 aaaaaggata tcgagttatc atgactcaat atccttttct tttattttga aaacttatac
    28501 tcaatgaaaa tcaaagagca aactaggaag ctagccgcag gctgctcaaa acactgtttt
    28561 gaggttgtgg atgaaactga cgaagtcagt aaccatacat acggcaaggc aaagctgacg
    28621 cggtttgaag agattttcga agagtattag ttgcctttaa aggcatccac catcgtttga
    28681 aattcttcat ttgagagagt aatccctttg cccattttag tatggtctgg actccaagca
    28741 cgaatatcaa actttgcagg ggcaccatta aagctcacac ggttaatttc cttggtccaa
    28801 cctttttcgt tttcagaaag agtcaacaag tgctcttcga tttcaaatgt aaattctgcc
    28861 attttcttct ccttttttag tttcattagt ttattcgtaa aatcttgtag attttaggaa
    28921 aattttatat aatattgata taaaagaagg gaggccaata tgagacataa attccagcaa
    28981 gttctaaata aaatacatga ttttttaaat ggatatgacc aacctgacca gactgaaacc
    29041 aactccctta cagccactat tgaagaggct atccagaaac aaaccgctgt tcaccttatc
    29101 ttgtctgaga caagctttac aggtgacatc atcaaatatg atcagcaagg ccagcaaatt
    29161 atcgtgaaaa atttttccaa aaatgtgagc cggattatcc gtataagcga tattcaacgc
    29221 ctgcgatttg tcccctcaac tgtccaaaca gcccaaaaaa atagatttaa gaaagagtga
    29281 gatgtagttg cttcatccca ctcttttttc ttagcgaatt tgttcaaaat gtaaatgaac
    29341 tgcgatatga tctccataac cacttctttc caagtcacgt tgtaaacgat aggaaatgta
    29401 gtgttctgca atggtaatgt aacctgcgcc caataaacga tgttcaacca tagattgaat
    29461 catactgata gtcgcacgtt ccaccttggc ttcttgtaaa tccaaaacta ccttcttagt
    29521 gacttgagca agattttgac gcaaatcatc tgtcaaaaca taaacagttt gggctgcctt
    29581 caagatggct tggtaaatct tatctggatt aaattcagca atttcgccat tacgtttgat
    29641 tacttgcata ggtttctcct ttattctttg ttttctttga tttctgccag cattttttct
    29701 tcttctactg tcagttgata atgttcaagt aaatccggtc tgcgctcgta ggttttcttt
    29761 aaactctcgt acaatcgcca ctgacgaatc ttttcatggt ggccactcat caatacatct
    29821 ggcacgacca tgcctcgata atcataggga cgtgtgtact gaggatattc taaaagacct
    29881 gaagaaaaac tatcatcttg gtggctagac tccttgccaa tcacttctgg aatcaggcga
    29941 actgtagcat caatcatggt catagctgcc aattctccac cagtgaggac atagtcacct
    30001 agggaaatct catctgttac caaggtctta atgcgctcat cataaccctc atagtgccca
    30061 cagataaaga ttagctcttc ctcttgagcc aaatcttcag cataagcctg atcaaactgc
    30121 tttccagcag gatcaaggag aataacgcgc ggatttttct tttcaatagc atcaaaggaa
    30181 tcgaaaatag gttgtgctct gagcaacatg ccctgaccgc ctccgtaggg ctcatcatct
    30241 acatgacggg ccttttcagc attttctcga aaattatgat actggatatc caagagccct
    30301 ttttctcgag cctttccaac gattgagtgc tccagtggag aaaacatctc tggaaagagg
    30361 gttaaaatat caatcttcat cgtctaaccc ttctaagatt tccacatcga cccgtttact
    30421 tggaatatca acattgagaa ccactggtgg gatataaggt aaaagcaaat cacgtttgcc
    30481 ttttcgtttg accacccaga catcattagc acctggttgc aggatttcct tgatggttcc
    30541 aaccaagcta tcaccctcat agacttccaa accgataatc tcgtgatagt aaaattcacc
    30601 atcgtctagg tcattcaaat cttcctcagc gaccttgaga ctgtatccct tgtacttttc
    30661 gatagtattg atatggtaca tatctttgaa tttaataatg tcaaagttct tctgtttacg
    30721 gtggctagcg atggtcactg tttggacaaa ctgatctttt tcatcaaaca aaaccagctc
    30781 agctcctttt ttaaaccgtt cttctgcaaa atccgtcaca gacaagactc gcatctcccc
    30841 ctgtaatccc tgcgtattaa cgattttccc aacattaaag tagttcatct tgtctcctgt
    30901 aatctccttt tttccatctt attctaacaa ttctcgaata atagccgcaa ttttttccga
    30961 ttctgaccat tgtaaataat ggtgattccc tcctaaaatg agtttagtat tggaagtcca
    31021 atattctgat tctctgtact ctttttctct ataaggctga caaaaaacaa atacaggaat
    31081 atgagcttct atagatacat cctcaaaatc ttcctcagta atctctccag atatctgaaa
    31141 ttctggatct tgattttcca actctaagcc tttttcttgc attaattccc agattttttt
    31201 attcgtttca ggactaaatg ttgcttgagt taagttctta aaataaagtt caggaccaca
    31261 ctcgtcaatc agcctcatct gctcttccat ttctggataa ggattttctg aaaaatcagc
    31321 aaacatgact tttttagttg tcggttcaat tgctactaaa gtctgacgct taattggttt
    31381 ctcgagtaat ttgcaagcta aaattccact ccaactatgt gcacaaagta tatattcaga
    31441 aattcctaat tcttcaagta cttcataaac cgcatctgca agattatcta gattttttcc
    31501 agcttggtca tgaatcggac tcctacctgt gttcggaaaa tcaattgtca aataaccaat
    31561 tgtaggagga ggtttttcaa gtataagtga aaaattttca taacttggta gcaaacctgc
    31621 tccgtttaaa caaactagca ctttcttttg cttttgataa gtaacagaga ggctaccaat
    31681 ttctgtagat acttcaaacc tcttcataaa gaaatccact gattctatat aatgaattat
    31741 taaaaatcct tatcctttat tttatcacgt tccaaggatt ttctcaagtt ggaggaaggg
    31801 gacaatatct ctactttccc ttcaataatc cttccaaatt atgtttatgt tggtaattaa
    31861 tggctgcggt tttgtctttc tcaaagacag tcttggtaag gtcaatatga ttaatagcta
    31921 cgattgcgac ggtgtagtaa atgatatcag ccagttctct ggcaagttcc tcgttcgaat
    31981 cctatccctt cttttcgacc agagcgccta ttcaaaacct cgactacttc tccgacttcc
    32041 tccactaact tcataaagag accttcatca gtccgagact gctgttaatg ttcgattaag
    32101 tagtcttgga attgcctaaa cgttcaatct tttatagtat attgaaacta gaatagtaca
    32161 cctttacttc taaaacattg ttagaaatcg atttgactgt cctgatcgat ttgtcctgtt
    32221 cttgtttcat tttactatat cttctattcc acacaaaaaa gcgagacatc cgtcccgccc
    32281 ttcttatttt tcgtcaataa cgattcttac ttttttgtat tcagttggga cagagtagac
    32341 aatcgttctt atcgcagaaa tagtgcgacc cttacgaccg attacacgac ccacatcgct
    32401 ttgatcaaga ttcaaatgat attccaaaaa ttctggtgta tcctcaatct tgatagttaa
    32461 ggcatctggt tgtgaaatta agggtttcac aatcgcaata atgagatttt caatcgtatc
    32521 catctgtcaa cctactttaa acttattttg aaaatttaga atcgtggaat tttttcaata
    32581 cgccttcttt tgaaaggatg ttacgtactg tgtctgaagg ttgagctcca ttagccaacc
    32641 atgcaagaac gcggtcttct ttcaaagtta cttggttttc agcaacaagt gggttgtaag
    32701 ttccaactgt ttcgatgaaa cgtccgtcac gtggtgaacg tgaatctgct acgttgatac
    32761 ggtagaaagg ttttttctta gaacccatac gagtcaaacg gattttaact gccattttta
    32821 aagtctcatt tctttaattt tttatttcgg tgaaatagct gagctattta gcacatgttc
    32881 tattatagca gatttctggc atgtgtcaag aaaaaaactt gacagactat aaatttctta
    32941 aagtatttag aaatttgtta gaataggaaa taaatgtttg aaagatgcta tccgtgaata
    33001 ctattttaga aaaattttat aaagaacacc aagtcaaacc cttcatatcg cctgaacgag
    33061 agttggatac ttggcttcta agtcccaagc ccgttcccaa gcgaaatatg gacttattag
    33121 tagatgattc actagcagga gatatcattt tactatggag aatccagttt ggtactttta
    33181 ccactgaaac atgattttaa aaactcccat tcgaatatga ttcaaaattc gcttagaatc
    33241 aatgttttcc cactttttca aaaaataaat tttcatctaa tttaaataac tttcaattaa
    33301 gtgtgaattt tgcttaaaat cattaaaaaa caccccatgg tgtgaaccat gaagtgttgt
    33361 aaatgctgtg ttgtagctag ttcttagcgt ttagagaact ggctagcttt acgagctttc
    33421 ttaagatctg gtttccaaaa tactttgagt acacttatgg tattgatgcc cctaaacatc
    33481 taaaaactct ggtagaaaaa gggtatgctc ttgtcgaaac ttcttttgac tcacttgacc
    33541 atctaaataa taccatgaaa aagaatatcc tgaagaagaa gggagttact ggactttcta
    33601 aaatgaaatc ttgcgacctg gatcaagccc ttcatgagca tttttcagaa gaagaattag
    33661 ctggtcactt tcatgtcctt ctatggactt tttttacaat ggcattgcta tcacacccaa
    33721 tacctatcta agcgcctggt tcgtaaactt tattgcagct cttcctctaa atttcctaat
    33781 tgttgaacca attgcccgtt ttatactaag ttcttttcag aaaccattta ctggggaaga
    33841 agttgaagat tttcaagatg atgatgaaat cccaactatt atctaagcca gttctgtaaa
    33901 ctactaatat ttgaaatcca cttcctttta gggtacaatg gttataaatg aatttttgag
    33961 aggatcagaa tgaaaaaact agcaaccctt cttttactgt ctactgtagc cctagctggg
    34021 tgtagcagcg tccaacgcag tctgcgtggt gatgattatg ttgattccag tcttgctgct
    34081 gaagaaagtt ccaaagtagc tgcccaatct gccaaggagt taaacgatgc tttaacaaac
    34141 gaaaacgcca atttcccaca actatctaag gaagttgctg aagatgaagc cgaagtgatt
    34201 ttccacacaa gccaaggtga tattcgcatt aaactcttcc ctaaactcgc tcctctagcg
    34261 gttgaaaatt tcctcactca cgccaaagaa ggctactata acggtattac cttccaccgt
    34321 gtcatcgatg gctttatggt ccaaactgga gatccaaaag gggacggtac aggtggtcag
    34381 tccatctggc atgacaagga taagactaaa gacaaaggaa ctggtttcaa gaacgagatt
    34441 actccttatt tgtataacat ccgtggtgct cttgctatgg ctaatactgg tcaaccaaac
    34501 accaatggca gccagttctt catcaaccaa aactctacag atacctcttc taaactccct
    34561 acaagcaagt atccacagaa aattattgaa gcctacaaag aaggtggaaa ccctagtcta
    34621 gatggcaaac acccagtctt tggtcaagtg attgacggta tggatgttgt ggataagatt
    34681 gctaaggccg aaaaagatga aaaagacaag ccaactactg ctatcacaat cgacagcatc
    34741 gaagtggtga aagactacga ttttaaatct taaaaaccaa aaaaatacag tatccacatt
    34801 cggtactgta tttcttttac tctcattctt aagttaaatt attaaaatcc catatttggt
    34861 ctatccagcc ttcataaaag tctggctcgt ggcagaccat aaggatagat cccctatatt
    34921 ctttgagagc gcgtttgagc tcatcctttg catccacatc caaatggttg gtcggctcgt
    34981 ccagcactaa aacgttgttt tcacgattca tcaagagaca gaaacgaacc ttggcttgct
    35041 ctccccctga taatacttga atctggcttt caatatgttt ggttgtcaaa ccacaacggg
    35101 caagggctgc acggacttct gcttgattaa gggcaggaaa ggcattccag acagcttcaa
    35161 gaggagtttg gcgattaccg ccttctactt cctgctcaaa ataaccaagt tctaaataat
    35221 ctccacgctc cacttcccca gcgattggcg agataatgcc caagagactc ttcaagagag
    35281 ttgtttttcc aataccatta gcaccaataa tcgcaacctt ttgattgcgt tcgaaggtaa
    35341 gatttaaagg cttagtaaga ggacggtcgt aaccaatttg caagttcttg gcttggaaga
    35401 taaagcgccc tggtgtacga gctggtttga aatcaaagga tggttttggt ttctcacttt
    35461 ggagttcgat aatatccatc ttatccaatt tcttttgacg agacatagcc atattacgag
    35521 ttgcaacacg ggctttatta cgagccacaa agtccttgag gtctgcaatc tctttctgct
    35581 ggcgttcgta ggctgcctct agctgagatt tcttcatagc ataaacttct tggaactggt
    35641 agtagtcacc agagtaacgc gtcagctgtt gattttccac atgatagaca atattaataa
    35701 cgtcattgag gaatggaata tcgtgcgaaa tgagaacaaa ggcattctca tagttttgga
    35761 gatagcgctt gagccaatca atatgctcag catccaagta gttggtcggc tcgtccaaca
    35821 gcaagatatc aggcttttca aggagaagtt ttgccaaaag caccttggtt ctttgcccac
    35881 ctgacaaaga agttacatcc gtatccatgc caaagtccat aacaccaaga gcacgcgcta
    35941 cttcgtcaat cttagcatcc aaggtataga aatcacgact ctccagacgg tcttgaagtt
    36001 ctcctacttc ttccatgaga gcatcaacat ccgcgccgtc ttcagccatt ttcatataga
    36061 ggtcattgat acgagcttca gctttgaaaa gctcatcaaa agccgtacgg agaacatcac
    36121 gcaccgactg tctttcagca aggacagagt gctgatccaa gtaaccagcc gtcacatatt
    36181 tggaccactc aacctttcct tcatctggca gcattttacc agtcacgata ctcataaagg
    36241 ttgattttcc ttcaccattg gcaccgacca ggccgatatg ttctcccttg aggagacgga
    36301 aggacacatc ttcaaaaatt gcacggtcac caaaaccgtg actcagattt ttaacttcta
    36361 aaatactcat tttaattcct taccttgttt ttatgtaatc gtttataaag gagccaagcc
    36421 agatagccac ccaaagtgtt ggtccacaaa tcatcaatct caaagacgcg attgaaatca
    36481 aagaaaaagt ccaagattaa ttgcgtacac tcgattccaa gactcacaag aaaactaaaa
    36541 agaaggacct tttttgtttt ccgcaaattt ggaaatagat aaaggagttg gaaaatcaga
    36601 ggaaaaaaca agaagacatt gaggatattt tgtaaaaaaa tccaacataa ttgtccaatg
    36661 tcactcactt cgcccagttt ccagagagaa ttgaaaggag tcaaaagaaa aaccaggcgt
    36721 ccaagatgct gaatacctgg agttcccact cccacggtag attgttcttg aggagtaaag
    36781 caaaaacaga caatgcaaat gctatagaaa atgactcccc agaccaaaat atgattataa
    36841 gtcttcttca tcattaagga tttaccgctg cgactgcctt ctggcggtca cgtttcattg
    36901 tgttagagcg caattgtcca caagctgcgt caatatctgt accatgctct tgacgaacca
    36961 cacagttgac cccttttttc ttaagcgtat catagaaagc caacacgcac tctttgggac
    37021 tacggctata ttggtcatgc tcactaactg ggttataagg aatcaagttt acataagaca
    37081 atttcttgat gttcttgagc aattcagtca attccaaggc ttgttctaca ccgtcgttga
    37141 cttcattaag catgatatat tcaaaggtta cacgacggtt tgttgtctca atgtagtatt
    37201 caatagcagc aaagagtttt tcaatcggaa aggcacggtt aatcttcatg atacttgaac
    37261 gaagttcatt gttaggtgcg tgaagagaca cggcaagatt gacctgaacc ccttcatcag
    37321 caaagtcacg aattttatga gccaaacctg aggttgaaac cgtgatgtga cgagcaccga
    37381 tagccattcc tttatcatca ttgatagtac gaaagaaatt caagacattg ttgtaattat
    37441 caaagggctc accgattccc atgacaacga tatggctgat gcgttcatcc tgaccacgct
    37501 catcaaagta tttctgaacc agcatgattt gcgctacgat ttcaccgtta ttgaggtcac
    37561 gttgcttctt aatcaaacca gaggcacaga aggtacaacc gatattacag ccgacctgag
    37621 tggtcacaca gacagataaa ccatagtgtt gacgcatgag tacagtctca attaacatac
    37681 cgtcgggcaa ttcaaagaga tatttgactg taccatcagc agactcttgc acaatacgtt
    37741 gtttcaaggg attgaccaca aactggtcat tgagcttagc aatcaaatcc ttggaaaggt
    37801 tggtcatttc ttcaaatgac tgcacacgtt tacggtagag ccattcccag atttgatctg
    37861 cacggaattt cttttctccc tgctccaata cccattcctg catggtttga tgtaccaaac
    37921 tatgaattga gggtttcatt tcttctcctt attctctact cacttctgac gaatgacaaa
    37981 atgacgttgt cccttgtcgt ctttctgacg acgtctattt ttcttatctg cattcgactt
    38041 tcgtttagtt tgagtcggtt tctttccttt tctagaaggt gtttcttctt ccgtcttacg
    38101 cattttcttg tcaaatgatg ctcgcttagg ggcttcattt tctaagacaa aataggcaca
    38161 accataacta caatactcta aaaggtagtc ttgtaaacga ctgatttttt caagtttttc
    38221 ttctgttcgg tcatccttgt aaaaacctcg taggcgaagc tgttcgttgc tccagtcccc
    38281 cacgatataa tcaaacttgg ttaatacttc tgaaaaacgc tgattaaaag tcgtcacatc
    38341 aaaggcatcc ttgatatttt caaccaagga aaaagctatc ccttccgttt cgaccttgtc
    38401 cccgtgtaaa tggaactccg gaccaggaaa cttgttatag ttgtataatt caggtgcaat
    38461 ttcttttcgc atagatatcc ttttttcacg attacttaat actttattct accataattt
    38521 ctagcagtta gcacgtttct cataaaaatg aaaaaagtct gacgattttg tcagaccaga
    38581 atcttataac ctaaaaagag aagaacaatt cttccctcca actatcatta tttagcagct
    38641 gcgtacaatt catctacttt attccagttg attactgaaa agaaagcttt gatgtagtca
    38701 ggacgcacgt tgcggtattt cacgtagtaa gcatgttccc aaacgtccaa gcccaagatt
    38761 ggttttttac cttctgagat tggtgtgtct tggtttgctg ttgaagtcac ttcaagtttc
    38821 ccttctttgt tgacaaccaa ccatgcccaa cctgaaccaa aacgagttgt tgctgctgca
    38881 gtgaaggctg cttggaattc ttcaaatgaa ccaaatgttg catcgattgc tgctgccagt
    38941 tctgctgaag gagctgtttt ctcgggagtc atcaattccc agaaaagagc gtggttcaag
    39001 tgtccgccac cattgttgat aagtgcttga cggatatcag ctgggataga ttctacatca
    39061 gcaagcaagg cttcaaggtc ttcaccgatt tcagggtgtt tttctaaagc tgcattggca
    39121 ttgttgacat aagtttgatg gtgtttgtca tggtgcaaat gcattgtttc cgcatcgatg
    39181 tatggttcca aagcgtcgta tgcatatgga agttctggta agataatagc catctgtaat
    39241 acctcttttt ctttctatat gaaaatgata acgcaaacat tcactttttt caagtttttt
    39301 gcttatagat aaggaaatca ctgacatact ttctaataaa ataagcaaat cagcaagaaa
    39361 cccacgacaa gatagtcctg acgcgggttg tagtttcaac aaatgtcaat tgacctgact
    39421 agcaatctgt aagagtgcct tttcaaaaag gaaacctttt tcataaagac ctgtcttaat
    39481 ctgatagtct gtctcaatca aataggaaat agcttgcttc aaaaagctca aagaaagtcc
    39541 tctcgaatct cttaatgcaa acttgatttg ataaggattt gggttacgtc ccagataact
    39601 acctaaacta cttgcaatct gcgattctgt ttggccagac tccgccaaaa tcttcacctg
    39661 agtaaaagtc cgaaattgtc ccagcatgac tgcaatcagt ttgatttcat cttccccttg
    39721 caaggtcaag tctctcacca aatcgcgcgc ctgatccatc tttttagtca gaataaactg
    39781 agttaaatca aaaatattgt cctgcaaggt cttgggaatt gcgttaacaa tatcctcttc
    39841 ctcaataaca gaattcgcct tataggactg taaaaagaga agatttttct ggatttcgct
    39901 aaattgaaac cccgacttga tgaggagatt ttcaaaagaa tgattggtaa actgcagacc
    39961 ttgtttctga ctccactttt ggaagtactg gcgcaattct tgttctttta cttctactgc
    40021 atcgaaggcc ttggcatcac gcttaagtaa tttgactaac cgtcttttgc tatccagctt
    40081 tccttctgca aagattatca acttggttgt tggagaagga ttgtcaaggt attcctcaaa
    40141 tgacttaagc tcatcatctg tcaaaaagcg tttcttagca gtcgtgatat ccataaaata
    40201 atctaatatc acgattttct catccgcaaa gaaaggaagg ctgaccaact ccagttccac
    40261 atccttgtaa actacttctt tcatatcaaa gtaggcaaag ttgaggtcag cagaatcata
    40321 cccaatctgt ttcaacattt gactcttcat cacttcaaac tgaccctgat ctgtccctgt
    40381 aaataggctc aggctcggta aatttgataa agtcaacttc tgactttctt caatggctag
    40441 catcgtctct cctttcttca gatttttcga tttaatttag tcaatatagc gcaatttccc
    40501 acggaaatct tctaagctct cgtagccttt ttccaccatg attgctttca gttcattggt
    40561 aatgcggtca aaagcactga cgccttcttt gtgaagggtc gttcccacct gcaccatact
    40621 tgctccacag aggatgtgtt caaaggcatc tcgaccagtc agaacgccac ctgttccgat
    40681 aatttggatt tgaggattta aacgttgata aaaggcgtga acattggcta aagcagtcgg
    40741 tttgatgtat tctccaccaa ttccaccaaa accattctta ggccgaataa cgacagattc
    40801 gtcttctata tagaggccgt ttccgataga gttaacgcag ttgacaaact tgagcggata
    40861 tttgttgaaa atagctgccg cttggtcaaa gtgaacaata tcaaaataag gtggcaattt
    40921 aattccaaga ggtttggtga agtaagcaaa cacttctgcc aaaatccggt ctgttgtctc
    40981 aaaatcatag gcaatctgag gtttacctgg aacatttgga caggaaagat ttagctcagt
    41041 cagaccacga aaatcactct cttggacttt tttcaaaata gtatgggttt cctctggaga
    41101 catgccgacc agagataaga agaaagttcg gttcgactct ttttcctgca aatctaaaag
    41161 ataatccaaa taatagtcta agccattatt tggcaagccc atagagttga tggaaccaag
    41221 tggaacatct tggtagcgtg gctcaggatt cccctgacgg aagtccaagg tcgctgtctt
    41281 agtaacaaag gttcctgccg ctgagttttt gacctcttct aactcctcta tcgtcataca
    41341 agccacacct gctgcattca tcaagcaatt gtcaaactca aaaccagcaa tttgtgtttt
    41401 cgttgatacc atgattctaa tcctccagat ttcttttatt gctaatatta taccataatt
    41461 ttaattctag gtttactaga ttctgtggtt ctatctattt attcggaaaa gaaagtctag
    41521 tacagaagtc atgctatgga aacagctagt gtattagaag tttgaacaag gcttgggtaa
    41581 cagacttttg aatgaagtcc aagcaacaag gagaaatctc aaccatgagt ccattccatg
    41641 actctgttgc tctagaaaac cttgcaatag tcaagtgata acgaataaaa ctttggattg
    41701 aaatatcccc aagattatct attacgagga gaaatcttaa cctttatcat ttttaaagaa
    41761 atatagtaaa atgaaacaag aacaggagaa atcgatcagg acagtcaaat tgattgctaa
    41821 caatgtttta gaagttgagg tgtactattc tagtttcaat ctactataca tgtaaatctc
    41881 tactttcgca aaatggattt tcagaacagt atcccagtat agttaaaaac tataaaaaag
    41941 agaggacgtt cccctcttaa aatcttattt tactgcttct ttcaaagcat ctaccttatc
    42001 caaacgttcc catggaagat caatatctgt acgtcccatg tgaccgtaag ccgatgtttg
    42061 acggtaaatt ggacgcttga ggtccagcat ttggataatc cctgcagggc gaaggtcaaa
    42121 gatttgacga gccgcttttt caagttgact ttcagctact gttcctgtac cgaaagtatc
    42181 gatacgaaca gaaacaggtt gcgcaacacc gatagcatag gccaactgca cttctgcctt
    42241 cttagcaagg tctgctgcaa cgatattctt ggcaatatag cgagccgcat aagaggctga
    42301 acgatccacc ttagtcgcat ctttaccaga gaaggcacca ccaccatgac gagagtagcc
    42361 accataagta tctacaataa tcttacgacc agtcaaacct gagtcccctt gaggaccacc
    42421 gattacaaaa cgacctgtcg gattgataaa gaattttgtc ttatcatcaa gataagaaga
    42481 tggaataact tctttgatga ccttgtcaat cacatcttga tggatttgtt cattagtggc
    42541 ctctggatca tgctgagtag aaataacgac tgtatctaca cgtaccggac ggtcattttc
    42601 atcgtactca actgtaactt gtgattttgc atctggacgg agatagctaa tttctccaga
    42661 cttacgaagt tctgccagac gacgaaccaa tttatgactg agtgcaattg gcaatggcat
    42721 aagctcttct gtttcatcta ctgcaaatcc aaacatgagc ccttggtcac ctgctccaat
    42781 caagtccagt ggatcttgat cagcatttcc acgaacctcc aaggcttcgt taacaccttg
    42841 agcgatgtca ggagattgtt ccaccaaaga tgggtgtact cccaccgtct cagcagaaaa
    42901 tccatattct gtattggtat aaccaatctc tgcaatggta tcacgaacca cacggttaat
    42961 atccacatag gcatttgtag aaatttcacc aaaaacgtgg acagaaccag tatatacagc
    43021 tgtttcagca gcaacgtgcg cctctggatc ctttgctaaa atagcatcca aaatcgcatc
    43081 tgaaatttgg tctgcaatct tatccggatg cccctcagat acagattcag acgtgaataa
    43141 tttacgctct gacataaaaa tgtcccccct taaaaaatat tgttatagag ttacaaagta
    43201 ctgataggaa aactcttcgt aataaatacc taccatctta tcgggactat ataactttat
    43261 cattatacca tttttaatcg caatttgcag tctttaatgt agtgtttcgg tttagatagt
    43321 tcgttctcat aaataaaaaa gttggagctc aatactccaa caatttctat taaaatgttt
    43381 ggcgtttagc tttacgctct gcacgaccgc gagcgcgatt ttcagcacgc ttggttttac
    43441 ggcgcttttc atcaaccgcc cattgaattt tcttcttata acccggtttg acttttttct
    43501 ttttcttttt aaccaaacca atcatttcga tatcaagttt atcttgtttt ttctcacggt
    43561 tggcacgacg atcacggtca taggtatctt gaaattcccc gtctttgacc atcttaggac
    43621 taaacttgat tcccaatttc tccaactcac ggatatccga gtcatcactt ggctgataaa
    43681 gggtaatagc tgtacctggt aggccatttc gtccagtacg accaacacga tgaacaaaaa
    43741 aagataagtc ttgcggaatg gcatcattga tgacatggct gacaccttca atgtcaatcc
    43801 cacgcgctgc caaatctgtt gcgacaatat actcaaaatc cagattttgc acctgattca
    43861 tgattcgctt gcgttcacga ggggcaatat cgccatggat ttttgcaacc ttcaagcctt
    43921 gagcagtcag atatgaatgc aattcatcag cacgcgtttt agtgttaaca aaaatcattg
    43981 ccaaatacgg ctgcatcaac tgagtcaact ggtaaatttg agcattctta tcatgtccct
    44041 tggtcgaaat caaccaatta tcaatggtgt cagaaataac cgttttggtc ttaattttct
    44101 ccataacagg atttgataag tattttttca agaatggttg cagtttttgt gggatagtcg
    44161 ctgagaagac catgaattgc aagtcttttg gaagactgcc agcaatctta tcaacagttt
    44221 ccaagaatcc catatccaag gtcatatctg cttcatcaac aacaaatgtc ttggctttat
    44281 gaatagctaa atcaccagat ttaaccaagt cgtagatacg gcctggtgtt ccaataacaa
    44341 tatgaggctg attgcttgcc aatttctcaa tctggcgagc cttatccgta ccacccacat
    44401 aattaaccac acgaacttcg acatctgagt gagctgaaat ctgacgcgct acttggtaaa
    44461 tttgagtagc caactcacga ctcggtgcag taatcactgc ttgtacacta tcgctagctt
    44521 catctaattg ctggaaaatc ggtaacaaga aagtatgagt cttacctgaa cctgtttttg
    44581 attctcctac taggtcacga cctgccaaaa caataggaat caacttgtct tgcacctctg
    44641 ttggagttgt aaattttaac tccttcaagg cttctctaat atagttttta aattgaaatt
    44701 tcgtaaatga cataacatcc tcgattctat ctaccttatc aattatacca tattttattc
    44761 cattacagta gtctcactta tttaggctat ttccagtagc ttctctagta agaaaaggct
    44821 ggaattttat agttccaacc tcttttcagt tattatttcc agtttaatat agcattcaag
    44881 ccatagtgat cactcacttg tggactcttg ttaccatcaa atacgacatg taaattttcc
    44941 accgctaact ctttggtagt aaagacataa tcgattcgaa ggggttcagt gttccctttc
    45001 cagccatcaa tttcaggcgg aacagtatag ctaccacttt tctcttgagc aacttcaaat
    45061 gcgtcttgta agcctaatgg actagctaaa atagcttggt aaccttcctg tccagccgga
    45121 ttgttgaaat ctccagctag taaaagtggc ttgttcaatt ttttcaagac agcctcaaat
    45181 cgtgcccatt cttcttggaa acctttatcc caccaagaga gatgaacact ggcaactgct
    45241 agctccttgc catcgactac agtttcagct agggcaacac ggcgagtatg atagtctgtt
    45301 ggatcatcca catctgaaac caaaatttct ctggcttcaa taggtgtttt agacaagata
    45361 gccacacctt cgtggtagcg gttatagccg atatggttat aggcccaggt ccagtagtaa
    45421 tttttccctt gctcagacaa cttttcaacc aagagtctaa cataatggtc ttggtgaata
    45481 ggctcagctg ctggcaaagc ttgataaagg tcattaacct ccacctctga cgaggtcatc
    45541 tcctgattga tttcttgaaa acaaatcaaa tcatagtcct tttcaagaat atcttcaagc
    45601 aaaatctgga atttttcctc tgcttctttc tccatccaac tgtgagtatt gagtgttaaa
    45661 aatttcattc ttttctccca aaataagagg ttggaataca gcttccaacc tcaagtatat
    45721 tacaattcta ctttagcaac tgctgtttta gctgcaagag aacctgtttt ttctaactta
    45781 actgatttaa ttgcatcacc atttgtgaag acaactactg ttgaagtttc acgtcctgct
    45841 gcacggatag catccaagtc agctgtgaca aggagatctc ctgctgcaac tttttgtcct
    45901 tcagcaacat gaactgtaaa tggtttacct tcaagactta ctgtgtccaa accaatgtga
    45961 accaatactt caagacctgc ttccgtcaca ataccaaaag catgttttgt tgggaagatg
    46021 cttgacacag tacctgaaac tggagataca atgtttccat ttgcaggttc tactgcaaat
    46081 ccatcaccca tcattttttg agcaaatact ggatccttta cttgttccaa agcaacaact
    46141 tgaccgtctg ctactgagta aacttcctca gtaagatctt tgaagtgaac agtgttttgt
    46201 tgtgcttcag tcatttggct tggaagagtt tcaggaatga tttcacctga atcaaggata
    46261 tcttggatat cagatttcaa aatgtcagct tttggaccgt agatagcttg aaccccttgt
    46321 cctttcatga caagacccat agctccttct gctttccatt gctctgcatt tcctacttta
    46381 tctgcatctt taacagttac acgaagacga gtcatacatg catcaacatc aacgatgttt
    46441 acacgtccac caagaaggtt gataatgttt acagcttgag agcctgctgc aactttcact
    46501 tcgctgctgg tttcttctga accttcagca gtttcgtagt ttccgttgcg ccctggagtt
    46561 gcgtagttga atttttgaat catgaagttt gcgataaagt acatgattac agcaaagaga
    46621 acagttaccc aaacgaagtt aacgatatcc ataccaatac cagcactgat tgcaataggt
    46681 gtacgagtca agaactcgat tgaaccgaat gagtgcatac gtaggtttac gacgtcagcc
    46741 atagcgaagg cagcaccttg aacaagtgag taaacaagat acataggtgt tgcgatgaac
    46801 atgaacatgt attcgattgg ttcagtaacc cctgtcaaga atgttgcaag agctgttgca
    46861 atcatcatac ctttgtattt atgtttcttg tcagcatcaa cattacggta gatagcaaca
    46921 atcacaccca tcaagatacc gaatgaaccg atcatttgtc caactttgaa acgagctgga
    46981 tgtactgtat ctaacaagtg ttgatattga ctagcatcag tacctttaag gtttacaagg
    47041 tctgttaccc atgcaagcca tagtgggtct tgaccgaata cttgagtacc tttagctgca
    47101 ccagttaaaa tgtcataagt accaccaaga gctgtgtagt tcattgggat agtcaacatg
    47161 tggtgaagac caaatggcaa gagcaaacgt tccaaagtac catacaagaa tggtgcaaga
    47221 attggagcag tttcttgtga gttggcaatc cagataccga agttattgat acctgtttga
    47281 actactggcc agaaagcagc aagtagaatt gcagcgattg ctgaacgaag aataactaca
    47341 aatggtacga aacgtttccc gttgaagaat gaaagtgcat caggaagttt acggaagttg
    47401 tagtatttgt tgtaagcagt tgcccctaca aaacctgaga taatccctac gaataccccc
    47461 atgttcaagg ctggagcttc aagaacactg ataaagtaat cagcaacttt gattgaacca
    47521 ccaaagaaag tagttaccat agcatctgga tttttcaaca tatcgcctga tacaccaaag
    47581 attgtaccag tgatacggtt aatcaagatg aaggcaagac cagcggcgaa agcaccacca
    47641 gcacgttctt tagcccagct tcctccaatg gctagggcaa acaaaatgtg aaggttaccg
    47701 ataacccccc aaccgatttg ctcaagaatt ccacctgtga tgacaagtgg tgcaaaggtt
    47761 gggttaatca tcacgataga cttaccgatt gaaatcatca aaccagcagc cggcataacc
    47821 gcgataacta ccatcaaagc cttaccgaat ttttgccaaa attcgaaaga caagacattt
    47881 ttgaatgtat ctttcatcat tcaaatctcc tttattttta tttaggcaaa cgttttacga
    47941 aaacgtttgc actttctatg atttaattat accactttaa ttttttttgt aaaggctttt
    48001 ataaaaaaat tacacttttt tctaaaaaat ttctagtgcg ccaaatttca agttcaaatt
    48061 agccatcaag aagtcttctc tgggggactt ctttggaagc tcttttcttc tgcgcatctc
    48121 ttttcaaaaa ctcttgtagc aaaaaaggag attttacaat ctcctcataa aagcagctat
    48181 tttacctaaa tcttcaagaa tcggcgcatg gatattcctg atcccaatga accaatgaaa
    48241 accccaatca caaatagtag ggcaatcatc aacggactaa ataaatctgg actaatcatg
    48301 gatagatttt gccctaccaa cgatttgttg acagattggt aaacaatttg ataaacaata
    48361 aagaccaaaa cagatggtgc gatagctccc aataaaccga taaaggctcc ttctaacaag
    48421 aacggtccac ggatataact gtttttagct ccgaccaagc gcatgatttg aatttcgcga
    48481 ctgcgggaaa taatggtaat acgaatggta tttgaaatca agaaaactgc gataaaaatt
    48541 aacaaagcag caatccctag tccccaaaca cggataaatg aagctaactt gaagagtctt
    48601 tctgtattgg caccgccatc ttgaacctca gagacacctt caattttttt agcatcttcg
    48661 gctatagttt ttacatcatt tggagtgttt gcctctacaa tataggcatc atagagagga
    48721 ttggcatctc cttcaaagat tttccagtta tctcccatta tctcggttaa tttttcatat
    48781 tgttcttctt tacttgaaaa ggtaacactt ttaaccgtag acatgttctt caaagaatca
    48841 tataccttgt ggtagtcatt atttgtaaca gtttgacctt ctttttcaat tgtctgacta
    48901 ttatcttcca catcctttcg gatataaact actacacgga cattattttc aatatctgta
    48961 gctagtttcg ctgtattgaa aataacagat gcaaatattg ccaccaaggt caaagtaatc
    49021 atgactgaac tgacagcagc tactgtcatc caaccatttc gtttcaaact ttttaaggct
    49081 tcaaataaat ggcgaaaaaa tctactaatc atcgtatcca tactctcctt ttgattcgtc
    49141 acgaacgaca cggccatttt caatggcaat gacacggtgg cgcaaggtat ttacaatctg
    49201 gctattatga gtcgccatca aaatagttgt tccttgtagg ttaatccgtt ccaagagatt
    49261 cataatttcc catgaattat ccggatccag atttcctgtt ggctcatcag ctatcaatac
    49321 tttgggatta tttacaattg cacgcgcaat cgcaatccgc tgttgctccc cacctgagag
    49381 ttcatttggg aaagaacgaa ccttatgctt caatccaacc aagtccaaaa cttccatcac
    49441 tcgtcttttg atattacggc gattttcccc gattacttcc atagcgtaag caatattttc
    49501 atagacagtt ttctttggta acaatttata atcctggaag acaaccccaa cactacgacg
    49561 tagaagcggg acatctttct ttttgatctt aaccagatta aaaccagcaa ctgataggct
    49621 tcctttatcg atttttactt cacgatacag agaacgaata aaagttgact tccctgctcc
    49681 tgaaggtcct acgatgtaag caaattcccc cggttgaacg ctaaccgaaa caccgcgtag
    49741 agcagttgtt ccgttgtcgt attttttaac gacatctctc atttcaataa ttgacatgtg
    49801 agttcctttc tatcttagct aattcgccac ttgagataag catcgataaa accatctagg
    49861 tccccatcca taaccttatc tacctgagca acctcaaagc tagttcggtg atcttttacc
    49921 atagtataag gcgtgaagac ataagaacgg atttggcttc cccaagtgat ctcctttttc
    49981 tcacctttga gagaatctac ctccgcagcc ttcttatctt gctccatttg atagagctta
    50041 gcctgcaaca tcttcatggc acgatctcta tttccatact gggtacgatc tactgttgat
    50101 tggacaacaa ttccagttgg aatgtgggtt aaacgtacac ctgttgaaac cttattgacg
    50161 ttttgtccac cggcaccacc tgaacggaag gtatccatct tgatatcatc ttcacggatt
    50221 tccacttcaa tagtatcatc caattctggc atcacttcta cagatgtgaa agaggtatgg
    50281 cgacgtttgg cagagtcaaa tggtgagatt cgcactaagc ggtgaacacc catttctgac
    50341 ttgaggagac cataggcatt aggcccttca aatgataaag ttaccgactt aataccagcc
    50401 tcatcacctg cttggtaatc caacacttcc actttaaagc ctttagcatt accataacga
    50461 gtatacatac gaagcaacat atcaccccag tcctgcgcct cagtaccacc agaacctgga
    50521 tggatttcca agatggcatt gttgtggtca taaggttctg acaagagtag agtcatctcg
    50581 tagctggtca ttatcttatc aagttcggct aactgcgcta ccagttcatc atgcactgac
    50641 tcgtcttcag ccaaaaaatc caataaaatt tcgacttcat cctgcaactc ttccatctta
    50701 tggaaggtat tgtaagtgtt ttttaattca tttaattctt gcgacgtttt ttgggccgca
    50761 atattatcgt tccaaaaatc aggttctgtc atcttgtttt ccaagatggc aatctcttcc
    50821 tctagccctt cgaggtcaaa gagaccccct gaaagaagct aatttttcac gatttgcgtc
    50881 aattttctga cgaattactg aaatgtccat aaatactcct tttttacatt atttcattat
    50941 agcatatttt atcatttctt tccatctttt gctctaatcc ccttttcatg aaaaaagaag
    51001 cctttgggct tcccttctta caagactttt gctgaagtgc gagcgatttc ttccacttga
    51061 ataccatttg cttcaaattt ttctttcaag gctggtaaat caattgatcc atcgatttgc
    51121 acttcgataa tcaccttacc atccttacgc ggaatattga ctgtatggga gatattcaaa
    51181 ttttcttcta caatcaaaga aacaattttt ccaagaacac caacttcatc ttctgtaaca
    51241 aagcgtacac gaatcccttc ttcgccataa cctgcaattt caagaaaggc ttggaaaacg
    51301 tcacggtcag taataactcc gtatacttga tggttatcta cgacagggag aatactaatc
    51361 ttatttttca acatcagata agttgcatct tctagactag catagcctga gacagtgaca
    51421 acatcgcgaa tcatgacatc ttttactttt gtcttattca gaagataatt catctcatag
    51481 atagaaagac ttgttgcttt agatggactt gcttgtgcaa tggttccctc agtcaccaaa
    51541 ccaactaatt gatcattttc gataacaggc agacggtgca aaccttgctc tctcatcaaa
    51601 tctgctgcat gagatactgt tgtatctgga ctaatataaa ctaccttgcg ggtcataaaa
    51661 tctttaactg ccatgagact tctccttttc tattcttatc cctattatac aatctttttg
    51721 taaatctatc aaacgcttac atttcttttt cgaaattcag aaaagtatga ataagctagc
    51781 aaaaagagct ctgaaatcga gctctgtgaa tgaaggtaag aaatttttca ttcgattttt
    51841 tcaattatta gaaattaagc tttcaatgta gatgcagatt tgtttgcaat ctcaatctac
    51901 cgactaaaaa ggtcccccgg actatattga tgattacttc gtaatctcca tctgccgact
    51961 aaaacaatcc actggattgt tttagccacc tagatatgct tttctgactt cttctgatga
    52021 agcgagttct tttcctgttc ctgataggac gattctccct gtttccagta catatcctcg
    52081 gtcagagatt gcaagtgctt tattggcatt ttgttcaatc aagaggaccg ttgttccttg
    52141 cttctgaata tcttgaatga tatcaaaaat ttcttggata aagattgggg caagtcccat
    52201 tgatggttca tctaaaagaa gaagttttgg tgttgacatg agggcgcgtc ccatggcaag
    52261 catttgttgt tccccccctg aaagagtggc tgcatcttgg ttcttccgtt cttcaagacg
    52321 aggaaagcgt gagaaaacct tcttcaagtt agcttgattt tcttcacgat ttttctttaa
    52381 gaaagctccc atttcaagat tttccataac agtcaagcca ggaaagacgt ggcgtccttc
    52441 tggaacttgt gaaagaccac ttgccacgat tttctgagct ggcatttttt ggatttcttg
    52501 acctaaaaat tcaatctttc ctgaacttgg tcgaaccaaa cctgacaagg tgcgaagaat
    52561 agttgtctta cctgcaccgt tggcaccgat aagggaaaca acttctcctt cattaacttc
    52621 aaagcttaca tcacgaactg cttggatcat accgtaatgc acagaaagat tttcaacttt
    52681 taacatagac attaggcttc acctcctaga taagcttcga taacgcgttt attggtctta
    52741 atttcgtctg gagttccttg agcgattaaa cggccatatt caagtacgta gatacgttct
    52801 gttacttcca tgaccagatt catatcgtgt tcaatcaaca tgattgtaat cttaaactca
    52861 tctttgatac gacgaattaa ctcagtcaat tcggctgttt cctgtgggtt catacctgct
    52921 gctggttcat ctaagaagag aattttaggt tccgtagcaa gggcacgaac aatttccaaa
    52981 cgacgttgtt gtccgtagga aagattttta gcaagagtct ctgcatcacc atctaaatca
    53041 aagattttca acaattccaa agctttagcc tttaattctt tttcactctt gtaaaaagct
    53101 ggtaagcgta agaaactagt aaaaacatgc tgtttgtgat ggtttccaaa agcaatcaaa
    53161 acattatcta aaactgttaa atctttaaag agacggatat tttggaaagt acgtccaagt
    53221 cccaaagagg caatcttata aggtgatttc ccattcaaaa ggtgaccatc tagggttact
    53281 gttccctcgc ttggttcata aacaccggtc aaaaggttga aaagggtggt tttcccagct
    53341 ccgtttggac cgattaatcc aaccagttcc ccttcgttca attcaagagt cacatctcca
    53401 acagctgtta gaccaccaaa atgtttggtt aactgtttta cttcaagtaa tgccattagt
    53461 tttgttcctc cttcttagat tttttaaaga aacgtgatag gctcagttcc catgttccaa
    53521 ggagtccacc tggtctgaaa atcattacca ataccaaggc caaagcgtaa ataatcatac
    53581 gcacactagc aacatcttgg agaagcatat tcaaaattcc cagaacaata gccgaaacaa
    53641 tcgcacctgt aatggaaccg agtccaccaa atacaacaat aatcaaaacg ttgattgagt
    53701 tgatgaaggt gtaatctttc ggtacaacag acccgataaa tcctgcctga agtgacccag
    53761 caatacttgc agtaatggca ccaaagacaa aagcgatgat tttaatttta gtcgtattaa
    53821 ccccaactga ctcagcagcg atttcatctt cacgaacaga gagggttgaa cgaccaattg
    53881 ggctacgcaa gaagttcaag gttgcaatgg ttgtaatcac gacaaagaag taaaccattt
    53941 gccaagttgt aaagttagga atccctaaga tacctgccgc accatttgta aggcttccac
    54001 cattgatgat aaagatacgg ataatttcag aaacacccag agttgctacc gcaagatagt
    54061 cccccttcaa gcgcaaggtt ggaatgccga caagtaaggc aactgctcct gaaagcaaag
    54121 cccctacaag catagctcca aagaaggcac cgtaggttgg tgatttagaa ccaataatag
    54181 ctgctgcata ggcaccaatc gccatgaaac cagcatgacc aagtgaaaat tgtcctgaaa
    54241 aaccaacgat taagttgaga ccaacagcca aaataatatt aattccaatt tgttgtaaaa
    54301 tctgtacata gaatagatta agtactccga ctgaaaccag tacactaatc aagctatagc
    54361 cagctaacaa aaggagtaac catagaatat taacttttaa attttccttc atcgtttaca
    54421 ccttctcttt cacattctta ccaaggatac cagctgggcg gacaatcaag atcaacaaca
    54481 agattccata aacaatggca tcacggaaat ctgacatccc aaaggcagtc gcaaaggttt
    54541 ccaatagacc aatcacaaag ccaccaagag ccgcaccagg aataattccg ataccaccaa
    54601 gtactgcggc aacgaaagat ttaagacctg gagtaacccc catcaaaggc tcaagagagt
    54661 tataataaag agcaatcaga acaccagccg cacccgcaag agcagaaccc aaagcgaagg
    54721 taaagctaat cgtacggttt acattgatcc ccatcaattg cgccgcgtcg ctatctactg
    54781 atactgcacg catggctttc cccatcttag tcttttggac aatgacttgt aacaaaatca
    54841 tcaaaatcaa ggaaatggcc aaaatcatta actgcacatt tgttaagcta attggtccca
    54901 aatcatatcg aactgtttga atcgcttgag ggaaggcacg ggtattggca ccaaccagat
    54961 agaccattcc atactccaat aggaaagaaa ccccaatagc cgtaatcaaa acagcaatac
    55021 gagtagagtg gcgcaaaggt cggtaagcaa gaaactcaat cacgacacca agaatagctg
    55081 tcgctagcat agctacaata agcgctacaa agaaattcat ttggaaagaa ttgatcaaga
    55141 aataaccgat aaaggctccc atcatataaa tatcaccatg ggcgaagttg atgagcttga
    55201 taattccgta aaccatggta tatcctaggg ctaacagcgc gtaaacacta cctagaatca
    55261 aaccatttac gagttgttgg agcataagat tcactctttc tatttataat tccgagggtt
    55321 ttccctcact ttttgatagg ttcttatact caatgaaaat caaagagcaa actaggaaac
    55381 tagccgcagg ttgctcaaag cactgctttg aggttgtaga taagactgac gaagtcagtc
    55441 acatatataa tccaaggcga cgttgacgca gtttgaagag attttcgaag agtattaaat
    55501 atcgaaacag ggagtgagtc aaaggctcat tccctatttc aacatttttc tattatggtt
    55561 ttacaacttc tgctgcttca actttaccat tgttcatggt catcatgtaa gcagttttga
    55621 ctgtgttgtg gtctgcatcg aagcttgttt gaccagttac accttcaaaa tcttttgttt
    55681 tagcaaggtt attcttgatt tcacctgaat ttttagcacc ttttgctgcg tttgctacaa
    55741 ggtgaactga atcataagcc aaggctgcaa atgttgaagg ctcttcattg tacttagcac
    55801 ggtaagcgtc aaggaaggct ttagctttag ctgaaacttc tacagtagtt gagaagcctg
    55861 agataaagta gatgtttgat gctttttcag cagttgcttg ttgtacaaac tcctcaccgt
    55921 tgaatccatc accaccaacg attggtttgt caattcccat gccacgcgct tggtttacaa
    55981 ttttaccagc ctcattatag taaccaggaa caacgatagc atcaaagtct ttccctttca
    56041 tttttgtaag ggctgcttgg aagtctgtgt cacctgctac gaaagtttca tctgcaacga
    56101 tttcaccctt gtatgactcg cggaaagatt ttgcaatccc tttagcatag tcactggcat
    56161 tgtcagtgta aagaacaact ttcttagcat ttaatttttc agaaacatag tttgagataa
    56221 tttttccttg gaagctatct tggaaagttc caataaagag gtaatcttga cctttagtca
    56281 atccatcttg agtcgcactt ggtgagatca atggaacacc tgcttttgta gcgttcgcta
    56341 ccgcagctgc agtcgcacca gatgtcgcag gtcctacgac tgctgatact ttagattggg
    56401 ttacaaggtt agttgtaact gaagcagcct cagctgtttc agacttatta tctttatcga
    56461 ctacttcgat ttgttttcca tcgataccac ctgcggcatt gatttcatca acagccaatt
    56521 gggcaccttt ttgttcagct gttccgtatg cagctaaaga acctgattct tcaaagttaa
    56581 acccgatttt aattgtcttt tcctctactg agttaccagc agtgttgact gctccagact
    56641 tcacttctcc acaggctgca agaagtgcta cacttgcaag cgccacaaac gatagggcaa
    56701 attttttctt catctttttg tctccttatt tcttaaatag tctgttaaga atataccgaa
    56761 ttatcagaaa attgtcaatg ttttccttga actttttcga tgataacgtt ttcctatcga
    56821 taaagattgc ccacaaaggg tgtttccaac tcttggatat gacaaaccct aactttttta
    56881 ataaattttt ccttgctcaa gcgtccgacc aattgctcca cttcttgagt tggaacatag
    56941 agttgtaagt aacgatgttt cttggaatga taggtaatat ctccataatc ctgcagtttt
    57001 ttggcatcac gattatagta aagatagata atcaagccag agcgattgac tttttcaaac
    57061 atgataaatc ctctttctaa gaaatctctc cctattatac caaaaaaagc aaacccagtc
    57121 gagtttgcct tctatcatca ttagttcaat gaattgttgg ccataatttc atcaataaag
    57181 ccatattcaa gtgtttcctg ggcgctcatc cagttatcac gttctgcatc tgcatggact
    57241 ttttccattg actgacctga attttcagcc aagatttttt ccaaggtatt acgagttttg
    57301 agcaagtgtt ctgcagcgat agccatatca gtttgttggg taccaccacc tgtaccaccc
    57361 attggttggt gaatcatgta ttcagcattt ggaagcatga aacgtttgcc ttttgctcca
    57421 cttgatgcga tgacagtccc catagatgca gccattccca taacaatggt ttggacatct
    57481 gccttgataa agttcatggt atctacgatt gccaaaccag ctgaaacaga accacctggt
    57541 gtattgacat aaaggtaaat atcttttgta ctatcttggg catccaagaa aagcaattgg
    57601 gcaataacag agttagccat attgtcttca accggacctg tcagcataat gatgcggtct
    57661 ttgagaagac gtgagtaaat atcgtaagaa cgttctccac ggcttgtttg ttcaataact
    57721 acaggaatca ttcatttctc cttttgagtt ttaattttgt tggtcaaatg actgaagata
    57781 agactattat aatatcttgg tcaaaaaagg tcaaattttt gctctgcttt cattagacag
    57841 aaacaaaaac ccaacctcct ttcgtgactg gaaatacttt tccaagtcat tcttcttttc
    57901 gatcttattt tgtaccgaac aagcggtctc cagcatctcc aagacctgga acgatataac
    57961 cgtgttcgtt caaacgttca tccaaggctg ctgtaaagat ttctacatct ggatgagctt
    58021 cttgaagggc ttttacaccc tctggagcag atacaaggca gacaaatttg atatttgatg
    58081 cgccacgttt tttaagagaa tcaacagcca agattgctga gccacctgtt gccaacattg
    58141 ggtctactac aaaaatttga cgttggtcaa tgtcctcagg caatttcacc aagtattcaa
    58201 ctggttgaag tgtttcttca tcacggtaca taccgatgtg gccaacttta gcagctggaa
    58261 ccaagttcaa gagaccatca accatcccga tacctgcacg caagattggg acgatggcca
    58321 atttcttacc tgccaattgt ttttgaactg tttttgtaat tggtgtttcg atttccacat
    58381 cttctagtgg aagatcacga agtacttcat accccatcaa cattgcaatc tcatctacta
    58441 gctcacgaaa agcttttgta gaagtatctg tacgacgcaa gattgacaat ttgtgttgaa
    58501 tcagtgggtg attaataact tcaatttttc ccatttttgg aattccttct ttcaatttat
    58561 tcttcttatt ataccaaaaa acggtttaaa aatctttcta aaccatttat ttttgataat
    58621 ttttacatta gatcagcctc tttaagagct gtctgtactg tctcaagtgg taaatgggtc
    58681 aattctgtcc ctttttcttg ataaaggtat tgggcgtagt cgtccattcg gtactggttg
    58741 atataaacca cgcgcttgca gccgacctga agcaattgtt ttgtacagtt gagacaagga
    58801 aaatgggtta cataggctgt aaagcctttg ggaacaccac gctcagcacc ttgaaggata
    58861 gcattgacct cagcgtgaag ggtgcgaacg cagtggcctt caatgaccaa acattcgtga
    58921 tcaatacaat gctcagtccc tgacaccgaa ccattgtaac cagtggaaat aaccttatta
    58981 tcttttacca gaatcgcgcc cactttagca cgtttacaag tggaacgatt cgcaattagt
    59041 agagcttggg ctgcaaaata ctcatcccag gccagtcttt tttcagtcat ctcttttctc
    59101 ctttttctct attttttaaa aaatggtaaa cctaaatctg caatcttttc agctggtacc
    59161 ttcatgccat ccttgatcca ttttagaagg acagagacga tggctgagct ccagaaggaa
    59221 tgaagataag agctgacacc ttttgatttc ccatggtatt tttctagaaa ttcctgcatg
    59281 gcttggacaa agattttttc cagatggtaa tccaaggcca attgaattac tctagcttcc
    59341 tttctggcct cccggaaaag gtgaacccaa accaaataaa ggtctgtctt taaatcgtaa
    59401 tgatgcagct gttccataat attgtggaca gttcgtttaa agacgctctc taaaatttcc
    59461 tctttggagt cataattgcg ataaaaggcc gcacgcgaaa cacctgcacg tttgaccaat
    59521 tcagaaatac taatcttggt cagttccttt ttttccaaga gttgcaagag ggctgtttca
    59581 atggcttctc tggttaataa attggattct tggtttgatt ttctgagatt ttcaagagac
    59641 ttttcagaga ttctacgttc agacataaca ttttctttct acttgtcaca acagacggat
    59701 gatgcttttg tttcaagggt tttcatgata taattgtaaa aaaagacaga acctttgtca
    59761 atgtataact gacaaagatg gagaatttct atgacttgga agattattgc tgactctggt
    59821 tgtgattatc gtcaactgcc aacaccagct attaacacaa cctttgtaag tgtcccctta
    59881 accattcaag tagctgatca ggtctttgtt gatgacgcca gtctcgatat tgaccaaatg
    59941 atggaaacca tgtatgcaac tgcagaagct tcaaaatcgg cttgtccaag cccagatgac
    60001 tatttgcgag catttgaagg tgccaaaaac attttcctag taaccatcac aggtaccctt
    60061 tctggcagtc acaatagtgc tcaactagca aagaatattt atctggaaga ccatcctgac
    60121 actaagattc atgtgattga tagtttatct gctggtggtg aagttgacct actcgtagaa
    60181 aaattgaatg acttgattga ccagggcttg tcttttgaag aagtggttga agctatcacc
    60241 gcctatcaag aaaaaactaa gttgctcttt gtcctagcca aagtcgataa cttggtgaag
    60301 aacggccgtt tgagcaagct tatcggtacg gtcgttggcc ttctcaacat tcgtatggtc
    60361 ggaaaagcta gtgaaactgg gactctcgaa ttgctacaaa aagcaagggg atcaaagaaa
    60421 tcagttcaag ctgcctatga tgagttagta aaagctggat atgctggtgg ccgtattgtc
    60481 atggcccaac gcaataacga aaaatgttgt caacagctct cagagcgaat ccgtgaaacc
    60541 ttcccacaag cggatattaa aattctacca acctctggtc tctgcagttt ctatgcagaa
    60601 gagggcggtt tgctgatggg ctatgaaatt gattaattgc attcttgaca agaggtgacc
    60661 atcatctctt gtttttttgt ggtacaatag agctatgaaa cattttgata ctattgtcat
    60721 cggtggggga cctgctggta tgatggctac gatttccagt agcttttatg gacagaaaac
    60781 cctcctcatc gaaaaaaatc ggaaacttgg aaaaaaatta gctgggactg gtgggggacg
    60841 ttgcaatgtg accaacaatg gtagcttaga caacctgcta gctggaattc ctggaaacgg
    60901 acgctttctt tacagtgttt tctcccagtt cgataatcat gacatcatca acttttttac
    60961 agaaaatggt gttaaactta aggtcgaaga ccacggacgc gtctttccag ccagtgacaa
    61021 gtctcggact attatcgaag ctttggaaaa gaaaatcacc gaactaggtg gtcaagttgc
    61081 tactcaaata gaaatcgttt ctgttaaaaa agtagatgac cagtttgtcc ttaagtcagc
    61141 ggatcaaacc ttcacttgtg agaaactcat tgtcacaaca ggtggtaagt cttatccttc
    61201 gactggttcg actggttttg gtcacgagat tgctcgccat tttaagcata ccatcaccga
    61261 tcttgaggct gctgaaagtc ctttattaac agattttcca cataaagcct tacaaggtat
    61321 ttctctggac gatgtgaccc taagttatgg taagcatgtc atcactcatg atttactctt
    61381 tacccacttt ggtttgtcag gtcctgctgc cctacgcatg tctagctttg tcaaaggtgg
    61441 ggaggttctc tcactcgatg ttttgcctca actttctgag aaggacttgg ttacatttct
    61501 agaagaaaat cgggaaaaat ccttgaaaaa cgctttaaaa accttgttac cagaacgctt
    61561 ggccgaattt tttgtacaag gatatcctga aaaagtcaaa caactgactg aaaaggaacg
    61621 agaacaactt gtccagtcca ttaaagaact taaaattcct gtaactggaa aaatgtccct
    61681 tgcaaagtcc tttgttacca agggtggagt cagtctcaag gaaatcaatc ctaaaaccct
    61741 tgaaagtaag ctggtacctg gcctccactt tgcaggcgaa gttatggata tcaatgccca
    61801 cacgggtggc tttaacatca cttctgccct ctgtaccggc tgggtggcgg gaagtctgca
    61861 ttatgattaa agattaaagg ttgtgaagaa tgaacagaag agaggcagtc gaatttgtta
    61921 acatgtgtat gattaaaaac ggagataagg tcctggtcca agaccgagtt aatcccgact
    61981 ggtctggcat tacttttcct ggtggtcatg ttgaacgtgg cgaatccttt gtcgatgctg
    62041 tcattcgtga agtgaaagaa gaaactggtc tgatcatttc caaaccccaa ctctgtggta
    62101 tcaaaaactg gtatgacgac aaggattatc gttatgtcgt ccttttttac aagacagaac
    62161 actttactgg tgaactccag tcttcagacg aagggaaagt ttggtgggag gactttgaaa
    62221 atctttctca tctaaaactt gcaaccgatg atatgtctga tatgcttcgt gtgtttctag
    62281 aagaagatct cagtgaattc ttttactaca aaaacggtga cgactggctt tatgatttga
    62341 agtaaaaaat aggctaggaa attcctagcc tatttattct gcatttgtct gaacgtaaac
    62401 cgcaatggca tctgacgtgt actgggctgt ttcagaacca aacttgtcaa tgttttcctt
    62461 aaactctggg ttgtagacgt aacctttacc gatatgaccg aatacctcaa tagagcagtc
    62521 aaatccataa gtgcgaatgg cttgcaagag cttggctgct tgctcctggt tttcggttgc
    62581 tgttgcaggt aaaccaactt gaagattttg tgccaaagtt tgaaagactt ggttaaaggc
    62641 ggccgtagcc tcgtcttcgt gacctttttg gcgttcgagc gcttgtccca tgacttcttg
    62701 accatatttc tctaccgctt cttggtggta tttttgattg tcttgatagc taaatcccgt
    62761 gaatttttcc tcaatggtca tttttctttc tcctttttgt tcttgaatag ttttttgcaa
    62821 ggtggaaatc aaggtatcca gatgttgcct ttcgcgagtt agatagtcca actgcctagt
    62881 caaatggggc aataaatctg tcctttcttc ctttaacagc tctgctattt tctctaaaga
    62941 aaagcctaga tatttgtagt aaagaatgac ctgaaggcgt tccaaatcct cttgactgta
    63001 ggttcgatag ccgttttccg actttaaggg gaccaagagt cctatcttgt catagtgatg
    63061 cagggtcttg acagagacac ctgaaagctg cgcagcttct tttatatggt acatgatttc
    63121 ctccttacaa tgatagtata acccctcgcc ttaggtcaga gtcaagcgtt tttgagaaaa
    63181 ttttttaact tttttgagaa aggtgtgaaa acttgaaaat gattttcttg acagacctta
    63241 gaaaacatga taggatagtc aagtctatca atcaaacaga aggctatttg acaactcaag
    63301 tagctttttc ttattttgaa aaaggagatc agagtttaac tatgtcagaa aaatcacaat
    63361 gggggtcgaa acttggtttt attctagcat ctgctggctg gccatcgggc ttggttccgt
    63421 ttggaagttt ccctacatga ctgctgctaa tggcggtgga ggctttttac taatctttct
    63481 catttccact attttaatcg gtttccctct cctgctggct gagtttgccc ttggccgtag
    63541 tgctggcgtt tccgctatca aaacctttgg aaaactgggc aagaataaca agtacaactt
    63601 tatcggttgg attggcgcct ttgccctctt tatcctctta tctttttaca gtgttatcgg
    63661 aggatggatt ctagtctatc taggtattga gtttgggaaa ttgttccaac ttggtggaac
    63721 gggtgattat gctcagttat ttacttcaat catttcaaat ccagccattg ccctaggagc
    63781 tcaagcggcc tttatcctat tgaatatctt cattgtatca cgtggggttc aaaaagggat
    63841 tgaaagagct tcgaaagtca tgatgcccct gctctttatc gtctttgttt ttatcatcgg
    63901 tcgctctctc agtttgccaa atgccatgga aggggttctt tacttcctca aaccagactt
    63961 ttcaaaactg actagcactg gtctcctcta tgctctggga caatctttct ttgccctctc
    64021 actaggagtt acagtcatgt tgacctatgc ttcttactta gacaagaaaa ccaatctagt
    64081 ccagtcagga atctccatcg tagccatgaa tatctcgata tccatcatgg caggtctagc
    64141 cattttccaa gctcgatccc ccttcaatat ccagtctgaa gggggaccca gcctgctctt
    64201 tatcgtcttg cctcaactct ttgacaagat gccttttgga accattttct acgtcctctt
    64261 cctcttgctc ttcctttttg cgacagtcac tttttctgtc gtgatgctgg aaatcaatgt
    64321 agacaatatc accaaccagg ataacagcaa acgtgccaaa tggagtgtta ttttaggaat
    64381 tttgaccttt gtctttggca ttccttcagc cctatcttac ggtgtcatgg cggatgttca
    64441 catttttggt aagaccttct ttgacgctat ggacttcttg gtttccaatc tcctcatgcc
    64501 atttggagct ctctaccttt cactttttac aggctatatc tttaaaaagg ctcttgcaat
    64561 ggaggaactc catctcgatg aaagagcatg gaaacaagga ctgttccaag tctggctctt
    64621 ccttcttcgt ttcttcgttt cgtcattcca atcatcatca ttgtggtctt cattgcccaa
    64681 tttatgtaat caaaaaggac ttgagtagtg aactcaggcc ctttcttttt atggatggct
    64741 aacaatcaat tccaaacctt gcccttccag agtccaagct tcaacatcac ttggtaggat
    64801 aaagtggctg cctttttgaa ttggataatt tttcccgtca acagttagct gaccttgacc
    64861 agccaagaca ctcaataagc tgtagtcagc tgtcttttca aagtcaactt ttccagtaat
    64921 ttcccacttg taaactgcga agaaatcatt agatacaagg agagtggaac gcaaatcatc
    64981 tgctttaaca gttacaggac ggctatttgc tggctcacca atgttcaaga catcgatgga
    65041 tttttcaaga tgaagttcac gcaagttgcc tttgtcatcc ttgcggtcaa agtcatagac
    65101 gcgataggtg gtatcgctag actgctgggt ttcaaggatt aagatacccg ccccgatagc
    65161 gtgcatagtc ccgcttggta catagaagaa atctccagcc ttaacaggga ctttggtcaa
    65221 caagtcatcc cagttcttgt cctcgatttg ctggcggagt tcttcttttg acttggcatt
    65281 gtgaccgtag ataatctctg aaccttcatc cgctgcgata atgtaccagc attctgtttt
    65341 tccgagttcg ccttcatgct cgagtccata agcatcgtct gggtgaactt ggacactgag
    65401 ccagtcgttg gcatcgagga tcttggtcaa aagtggaaat acaggttctg gacgattgcc
    65461 aaataattca cggtgttccg catacaaagt agcaagatct gttccctcgt aacgaccatt
    65521 ggcaacttta gagactccat ttggatgggc tgagatggcc caatattctc cgattttttc
    65581 acttgggatg tcgtagccaa actcatcacg tagcttggct ccaccccaga ttttttcttg
    65641 cataactgat tgtaaaaata atggttctga catgtcgatc tcctgtctga tttttctccc
    65701 ctcattatag caaaaaaaga gttcgaattg aactcttttt tacatcttat aaagcaggga
    65761 gaagatttta taaaaatagt aaacaaatgt gctctacccg atgcttgcac cattgctata
    65821 aatgacatcc ttgtaccaat agaaggactt cttcttgcta cgtttgagag ctccgtttcc
    65881 tacattatct cgatctacat agataaagcc atagcgctta ttcatttccc ctgtgccagc
    65941 tgaaaccgga tcgatacagc cccaagtcgt ataaccaagc aagtcaaccc cgtcttggta
    66001 aatggcatct cgcatggcct tgatgtgggc ctctaagtaa gtaatccgat agtcatctgc
    66061 tacataacca ttctcatccg gtgtatccat agcaccgagt ccattttcta cgataatact
    66121 a
//
LOCUS       DAVKNL010000003        63482 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVKNL010000003 DAVKNL010000000
VERSION     DAVKNL010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 63482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63482
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.003"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(261..629)
                     /gene="rplL"
                     /locus_tag="VW411_000146"
     CDS             complement(261..629)
                     /gene="rplL"
                     /locus_tag="VW411_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEU9792958.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            complement(705..1205)
                     /locus_tag="VW411_000147"
     CDS             complement(705..1205)
                     /locus_tag="VW411_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEU9792959.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     misc_feature    complement(1244..1379)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(1454..2713)
                     /locus_tag="VW411_000148"
     CDS             complement(1454..2713)
                     /locus_tag="VW411_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEU9792960.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     gene            complement(3105..4856)
                     /locus_tag="VW411_000149"
     CDS             complement(3105..4856)
                     /locus_tag="VW411_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9792961.1"
                     /translation="MKRQTVNQTLKRLAVDLASHSFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFVDRQGSGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKKSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGSA
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     TSDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKVAIVGPTGAGKSTLINLLMRF
                     YPISSGDILLDGQSIYDYTRVSLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVI
                     AAAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATS
                     SIDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMD
                     RKGKYYQMQKAAAFSSE"
     gene            complement(4858..6582)
                     /locus_tag="VW411_000150"
     CDS             complement(4858..6582)
                     /locus_tag="VW411_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9792962.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGSAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VLVAILTIVIVGLSRLVNPFYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISVKRIEEVFVEAPEDIHSELEQKQATRDKVLQV
                     QELTFTYPDAAQPSLRYISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGFNQEVSDQELWQALEIAQAK
                     DFVSEKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNTSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYCEIN
                     ASQHGKED"
     gene            complement(6640..7578)
                     /gene="msrB"
                     /locus_tag="VW411_000151"
     CDS             complement(6640..7578)
                     /gene="msrB"
                     /locus_tag="VW411_000151"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU9792963.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLL
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            complement(7724..7798)
                     /locus_tag="VW411_000152"
                     /pseudo
     CDS             complement(7724..7798)
                     /locus_tag="VW411_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(7874..8743)
                     /locus_tag="VW411_000153"
     CDS             complement(7874..8743)
                     /locus_tag="VW411_000153"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEU9792964.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            complement(8745..10031)
                     /locus_tag="VW411_000154"
     CDS             complement(8745..10031)
                     /locus_tag="VW411_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEU9792965.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILALDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            complement(10182..10919)
                     /gene="mecA"
                     /locus_tag="VW411_000155"
     CDS             complement(10182..10919)
                     /gene="mecA"
                     /locus_tag="VW411_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEU9792966.1"
                     /translation="MKMKQISDTTLKITISLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEVKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            complement(11119..12153)
                     /locus_tag="VW411_000156"
     CDS             complement(11119..12153)
                     /locus_tag="VW411_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU9792967.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESDKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANNLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            complement(12182..13615)
                     /locus_tag="VW411_000157"
     CDS             complement(12182..13615)
                     /locus_tag="VW411_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEU9792968.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMI
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            complement(13631..14617)
                     /locus_tag="VW411_000158"
     CDS             complement(13631..14617)
                     /locus_tag="VW411_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9792969.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            complement(14619..15722)
                     /locus_tag="VW411_000159"
     CDS             complement(14619..15722)
                     /locus_tag="VW411_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9792970.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLTRRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYTSKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            complement(15712..16557)
                     /locus_tag="VW411_000160"
     CDS             complement(15712..16557)
                     /locus_tag="VW411_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9792971.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKKT
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGDL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            complement(16836..18110)
                     /gene="psr"
                     /locus_tag="VW411_000161"
     CDS             complement(16836..18110)
                     /gene="psr"
                     /locus_tag="VW411_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEU9792972.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            complement(18107..18955)
                     /gene="pheA"
                     /locus_tag="VW411_000162"
     CDS             complement(18107..18955)
                     /gene="pheA"
                     /locus_tag="VW411_000162"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU9792973.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     NYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESCPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            complement(18952..19428)
                     /locus_tag="VW411_000163"
     CDS             complement(18952..19428)
                     /locus_tag="VW411_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEU9792974.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIENRLGMSIANFFA
                     EKGETAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLRADFETLY
                     QRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            complement(19421..20704)
                     /gene="aroA"
                     /locus_tag="VW411_000164"
     CDS             complement(19421..20704)
                     /gene="aroA"
                     /locus_tag="VW411_000164"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU9792975.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKEYTRNHTEDMLKQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIAPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            complement(20796..21134)
                     /locus_tag="VW411_000165"
     CDS             complement(20796..21134)
                     /locus_tag="VW411_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEU9792976.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTDY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            complement(21145..22248)
                     /locus_tag="VW411_000166"
     CDS             complement(21145..22248)
                     /locus_tag="VW411_000166"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEU9792977.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSEIILDRIEDFKERLEAIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            complement(22258..23424)
                     /gene="aroC"
                     /locus_tag="VW411_000167"
     CDS             complement(22258..23424)
                     /gene="aroC"
                     /locus_tag="VW411_000167"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEU9792978.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIENDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            complement(23434..24501)
                     /locus_tag="VW411_000168"
     CDS             complement(23434..24501)
                     /locus_tag="VW411_000168"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEU9792979.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWEVGKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            complement(24520..25374)
                     /locus_tag="VW411_000169"
     CDS             complement(24520..25374)
                     /locus_tag="VW411_000169"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEU9792980.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLVETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            complement(25364..26041)
                     /locus_tag="VW411_000170"
     CDS             complement(25364..26041)
                     /locus_tag="VW411_000170"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEU9792981.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            complement(26038..27201)
                     /locus_tag="VW411_000171"
     CDS             complement(26038..27201)
                     /locus_tag="VW411_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEU9792982.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFISKDKVAFNQAFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLDY
                     ESAHVYGQEAPDFFNVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASHTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            complement(27888..29522)
                     /locus_tag="VW411_000172"
     CDS             complement(27888..29522)
                     /locus_tag="VW411_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9792983.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMIICFSNIAIHFPFEEIPIYFSSMIAILLVIAFSTSLTAFYNVFYESK
                     DLVSYRPYAFKESEIIIAKGLSVLLPALTGIVPILAYFLVLYIRLAPSLWLGLPLMLL
                     SLTLLFVSVALVMVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSTEALVGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVITVLFTSAFLPYL
                     MMIGLISKIRDSQIVPDIHPPYWLPLFFIALFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIYAVQSFLPVLTLLGLSLYLGLPIISMIYLLVVWIIASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAISLGVGALIVLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(29503..30252)
                     /locus_tag="VW411_000173"
     CDS             complement(29503..30252)
                     /locus_tag="VW411_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9792984.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLSRSDLEASLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVED
                     LRKDYPDQSLESIYLSLAGRKEEVADASQGH"
     gene            30431..31885
                     /locus_tag="VW411_000174"
     CDS             30431..31885
                     /locus_tag="VW411_000174"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEU9792985.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(31975..34593)
                     /gene="alaS"
                     /locus_tag="VW411_000175"
     CDS             complement(31975..34593)
                     /gene="alaS"
                     /locus_tag="VW411_000175"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEU9792986.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGS
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNASQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVDNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAIAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDVKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQPKIQELLDAVAGKL"
     gene            complement(34615..35100)
                     /locus_tag="VW411_000176"
     CDS             complement(34615..35100)
                     /locus_tag="VW411_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEU9792987.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSEISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(35375..35614)
                     /locus_tag="VW411_000177"
     CDS             complement(35375..35614)
                     /locus_tag="VW411_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9792988.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTAILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(35867..36937)
                     /locus_tag="VW411_000178"
     CDS             complement(35867..36937)
                     /locus_tag="VW411_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9792989.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(36934..37707)
                     /locus_tag="VW411_000179"
     CDS             complement(36934..37707)
                     /locus_tag="VW411_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9792990.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIITGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(37704..38510)
                     /locus_tag="VW411_000180"
     CDS             complement(37704..38510)
                     /locus_tag="VW411_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9792991.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVALLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(38491..39648)
                     /locus_tag="VW411_000181"
     CDS             complement(38491..39648)
                     /locus_tag="VW411_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9792992.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFEPEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(39707..40657)
                     /gene="murB"
                     /locus_tag="VW411_000182"
     CDS             complement(39707..40657)
                     /gene="murB"
                     /locus_tag="VW411_000182"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEU9792993.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARVVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESLSVAKMYAGGFTPCKR"
     gene            complement(40769..41521)
                     /locus_tag="VW411_000183"
     CDS             complement(40769..41521)
                     /locus_tag="VW411_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEU9792994.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLTSLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            complement(41531..42262)
                     /gene="budA"
                     /locus_tag="VW411_000184"
     CDS             complement(41531..42262)
                     /gene="budA"
                     /locus_tag="VW411_000184"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEU9792995.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHRAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFLHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            42404..43675
                     /locus_tag="VW411_000185"
     CDS             42404..43675
                     /locus_tag="VW411_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU9792996.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEERVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYSLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(43695..44510)
                     /locus_tag="VW411_000186"
     CDS             complement(43695..44510)
                     /locus_tag="VW411_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9792997.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVDAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKVQLTSLTNMGEAVNELQAGKIDAVHMDESVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVVQKLKDEGTYQSYLEKAASLTEVEE"
     gene            complement(44667..45320)
                     /gene="phoU"
                     /locus_tag="VW411_000187"
     CDS             complement(44667..45320)
                     /gene="phoU"
                     /locus_tag="VW411_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU9792998.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(45332..46090)
                     /gene="pstB"
                     /locus_tag="VW411_000188"
     CDS             complement(45332..46090)
                     /gene="pstB"
                     /locus_tag="VW411_000188"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9792999.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(46103..46906)
                     /gene="pstB"
                     /locus_tag="VW411_000189"
     CDS             complement(46103..46906)
                     /gene="pstB"
                     /locus_tag="VW411_000189"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9793000.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(46917..47801)
                     /gene="pstA"
                     /locus_tag="VW411_000190"
     CDS             complement(46917..47801)
                     /gene="pstA"
                     /locus_tag="VW411_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU9793001.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(47791..48708)
                     /gene="pstC"
                     /locus_tag="VW411_000191"
     CDS             complement(47791..48708)
                     /gene="pstC"
                     /locus_tag="VW411_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU9793002.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(48708..49586)
                     /gene="pstS"
                     /locus_tag="VW411_000192"
     CDS             complement(48708..49586)
                     /gene="pstS"
                     /locus_tag="VW411_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEU9793003.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(50160..51464)
                     /locus_tag="VW411_000193"
     CDS             complement(50160..51464)
                     /locus_tag="VW411_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEU9793004.1"
                     /translation="MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGHLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQIFGDQLYLLPEL
                     LPDLWKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGET
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(51465..52238)
                     /locus_tag="VW411_000194"
     CDS             complement(51465..52238)
                     /locus_tag="VW411_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU9793005.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRSLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(52228..52506)
                     /locus_tag="VW411_000195"
     CDS             complement(52228..52506)
                     /locus_tag="VW411_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEU9793006.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(52506..52907)
                     /locus_tag="VW411_000196"
     CDS             complement(52506..52907)
                     /locus_tag="VW411_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEU9793007.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(53117..53734)
                     /locus_tag="VW411_000197"
     CDS             complement(53117..53734)
                     /locus_tag="VW411_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEU9793008.1"
                     /translation="MKTKLIFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYENFFGYLYLKSRRK"
     gene            complement(53724..54497)
                     /locus_tag="VW411_000198"
     CDS             complement(53724..54497)
                     /locus_tag="VW411_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEU9793009.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKEKPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(54508..55245)
                     /locus_tag="VW411_000199"
     CDS             complement(54508..55245)
                     /locus_tag="VW411_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEU9793010.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEEYNLVWIIVEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAEQ
                     ELIHMMATFVLMDRDSRKVHVVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKA"
     gene            complement(55250..56380)
                     /locus_tag="VW411_000200"
     CDS             complement(55250..56380)
                     /locus_tag="VW411_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEU9793011.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(56464..56853)
                     /locus_tag="VW411_000201"
     CDS             complement(56464..56853)
                     /locus_tag="VW411_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU9793012.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQTKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(56869..57252)
                     /locus_tag="VW411_000202"
     CDS             complement(56869..57252)
                     /locus_tag="VW411_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEU9793013.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(57253..58041)
                     /locus_tag="VW411_000203"
     CDS             complement(57253..58041)
                     /locus_tag="VW411_000203"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEU9793014.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGFRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(58034..58969)
                     /locus_tag="VW411_000204"
     CDS             complement(58034..58969)
                     /locus_tag="VW411_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEU9793015.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(59099..59275)
                     /locus_tag="VW411_000205"
     CDS             complement(59099..59275)
                     /locus_tag="VW411_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEU9793016.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(59421..60122)
                     /locus_tag="VW411_000206"
     CDS             complement(59421..60122)
                     /locus_tag="VW411_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEU9793017.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLL"
     gene            60273..61301
                     /gene="queA"
                     /locus_tag="VW411_000207"
     CDS             60273..61301
                     /gene="queA"
                     /locus_tag="VW411_000207"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEU9793018.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKKNGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHSIQEHYRFFS
                     FGDAMFIY"
     gene            complement(61962..62351)
                     /locus_tag="VW411_000208"
     CDS             complement(61962..62351)
                     /locus_tag="VW411_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9793019.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTDWKQENGMWYFYNTDGSMATGWVQVNSSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNTSGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(62405..63097)
                     /locus_tag="VW411_000209"
     CDS             complement(62405..63097)
                     /locus_tag="VW411_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9793020.1"
                     /translation="MNTSFVHAADGIQYVRDDTRDKEEGIEYDDADNGDIIVKVATKP
                     KVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGYVTEQVTVD
                     RKEATDTVIKVPAKSKVEEVLVPFATKYEADNDLSAGQEQEITLGKNGKTVTTITYNV
                     DGKSGQVTESTLSQKKDSQTRVVKKRTKPQVLVQEIPIETEYLDGPTLDKSQEVEEVG
                     EIGKLLLLQSIL"
BASE COUNT        18799 a        14393 c        11902 g        18388 t
ORIGIN      
        1 acaatatcaa ctcccatgat agtcatgaga tgactcttca cgaattgacg tgatgactgt
       61 ccttcctttt gcataattac ctccgaaaca caaaaaaagg ggtagacaat ctagtgtcta
      121 cccccgaaag tttattaaaa caaaaatcct gccaaagaat ttttggcagg aaaccaaatc
      181 aatttatcag tttctatcaa tcgcttatcg ctctcaaaga ctggtaaata gggattccgc
      241 aatcaaattg cgatactcta ttatttaaga gtaactgaag ctccagcttc ttccaattta
      301 gctttgattt cttcagcttc tgcagttgca acgccttctt taacaagtgc tggtgcaccg
      361 tcaacaagtt ctttagcttc tttaagacca agaccagtga tttcacgtac aactttgata
      421 acgccaactt ttttgtcgcc tgcagatgtc aattcaacgt cgaatgaatc tttagcagca
      481 ccagcatcag ctgcatcagc tgcagcaaca gctacaggag cagctgcagt tacaccaaat
      541 tcttcttcga tagcttttac aaggtcgttc aattcaagga ttgaagcttc tttaatttca
      601 gcaataatgt tttcaatgtt caatgccatt gttatttcct ccaaataagt tttaaatttt
      661 ataatagttt ttttcgtagc taggctacgc tgtgtagctt aagattaagc cgcgtcttct
      721 ttgctttctg caaccgcttt gactgcaaga gcaacgttgc gcactggcgc ttgaagtaca
      781 gaaaggagca tagaaagaag tccttcgcgg tttggaagag ttgcaagtgc aagaatctct
      841 tctttagatg cgacagcgcc ttcgattgca ccacctttaa tttcaagtgc ttcagcgttt
      901 ttagaaaagt cgttcaagat tttcgctggt gcgataacat cttcattaga aaatgctact
      961 gcagatggtc caacaaatac agatgcaaga tcttcaagac cagctttttc agctgcacga
     1021 cgcaagattg agtttttaat aactttatac tcaacttcgc ttccacgaag ctcacgacga
     1081 agaactgtat cttgctcaac tgtcaaacca cgagcgtcta caacgacgat agatgcagca
     1141 gctttcattt tttcagctac tacgtcaact agttccgctt ttttagcaat aattgcttca
     1201 ctcattagtg tgttcacctc cgtaattatt ttgcttgggg aatttttcaa aaagaaaaac
     1261 gcgcccaaac ctagacacga aagtacaata cgcttctttt tacatgatac gttttgtcct
     1321 cggtaggata tttatgagtc gagctcccct actgtcttag gcagtttttt taaaccgtat
     1381 ataagtatag catagtcaaa aaaagaatgc aagatttttg caaacttttt taaaattttt
     1441 cgtaattttt cttttaaagt tctactgtca ggacttgacc ttgcttaaca acctgttctc
     1501 cggcgatata aacatcatct acatcactag atttaactgc ataaaccagg tgagacagca
     1561 tattttcctg aggttggaga tgaattttcc cttgtggttg aatgaccaga aaatctgctt
     1621 gcttgccgac ttccagactt cctatctgat tttccattcc aaggacctta gccccttcga
     1681 ttgtcagtac cttgagagct gtttcgattg gaaactggct ggcatcccca cttttcatct
     1741 tctgaagaag agctgcagtc cttccttcct caaacatatc tagattgtta ttggaagcaa
     1801 ccgagtcagt cgcaattccg actgctactc ccgctttttg gagctggata attggagcaa
     1861 ttcctgatgc cagtttgagg ttactgatag gattgtgggc gatagccact tgagaagatg
     1921 ccaagcgttc aatttctctc tcgtttaatt cgaccccgtg agcaaatacg gacggatgat
     1981 ctaaataacc cagttcttca agaaaagcaa gggggcgttt gccgtatcgt ttgaggataa
     2041 ttcctgactc ctccttggtc tccgccacat ggacatggag cggaatattt agctcttttg
     2101 ccatttccaa actcgcttcc agcaagtctc tactgcagct atacggagaa tgaggtgcta
     2161 ccataacctt gaaatttgga tttttatatt ttaagatttc gtctatgatg gatcgagttc
     2221 tacttatagt ctcagcagtt gtttctgtct ctgaagaaaa gagagtcgga gaaaaataac
     2281 aacgcatctt ggaagttttc accacctgat aaatttgctg gatatccaca ccattgggat
     2341 tatacatatc gttaaaggtt gttgttcctg actggagcat ctctgtcagg gcttctttga
     2401 ccgcattggt agtcatgtcg ggagtaaact cagattctgc tggccaaata tagtcattga
     2461 gccattcatg gagattgctg tcatctcgga tccctctcag acctgtcatt gcagaatggg
     2521 tgtgacaatt gaccaaacca ggcataatcc aagctccctg atagtctata atctgctcag
     2581 cttgctctaa aaacgctggc ttatcttgac cgacatagac gatttgagaa tccttgactg
     2641 ctaagattcc atcaagataa acatggaaat cttgatcaca agtcacgata tttacatgct
     2701 gaaagacttt cattataggc tccttttcta aaagacaagt tgtactctct tcaaaccact
     2761 tcagattcac tttgccgtag gtgtggttac tgacttcgtc agttctatct gcaacctcaa
     2821 aacagtgttt tgagctgact tcgtcagttc tatctgcaac ctcaaaacag tgttttgagc
     2881 tgacttcgtc agttctatct gcaacctcaa aacagtgttt tgaactgact tcgtcagtct
     2941 tatctacaac ctcaaagctg tgctttgagc aacctacggc tagcttccta gtttgctctt
     3001 tgattttcat tgagtattac agtgaacaat ttttctagct attgtaacaa aaaagtcact
     3061 cgaaggcaac ttttttcgtc cataaacttt caaaagagaa tggcttattc agaactaaaa
     3121 gccgcagctt tttgcatttg gtaatactta ccctttctat ccatgagttc ttgatggtta
     3181 ccatattcaa caatatcacc atctactaag acaagaatta aatccgcatc ctgaatggtt
     3241 gacaaacggt gagcaatgat gaaacttgtg cggcccttca tgagttttgc aaaggcatcc
     3301 tgtaccagca cttctgtccg tgtatcaatg gaagaagttg cctcgtctaa gataagaatc
     3361 tttggaatag ccagaaagac tcgggctatg gtcaagagct gagcttggcc gacagagaga
     3421 gattctccag cattttccaa cttggtatcg tatccctgtg gcaactgttg gatgaaaaag
     3481 tctgcattag ctgctttggc agcagcaatt acttgctctc gactggcttc aggattgcca
     3541 aaggcaatat tatcatgaat ggtcccttgt gtgagccagg tttcttgaag caccatacca
     3601 aactgctgtc tcaatgatac tcgtgtataa tcataaatgg attgcccatc cagcaagata
     3661 tctcccgagc taatgggata aaaacgcata aggagattga taagagttga ttttccagca
     3721 cctgtcggac caacgatggc taccttacta ccagctggaa tatcgataga caagtcctta
     3781 atcaaaattt tttcaggatg gtagccaaaa gagacatgtt taaaggaaat agctccctta
     3841 acttggtcac tggtcgtcaa gacttcctta cctgtttcag ccacttcagg gctatctaag
     3901 actccataga tacgctctac gcaagccaga gcactttgca actcagctag cactgaagaa
     3961 atatcgttaa agggcttggt gtattgctga acatagttca aaaaagtcac taaacgaccg
     4021 acggtcaagg ctgaacccat catgatacga taagctccta ctccagctaa aagggcataa
     4081 atgagtgcat ttacaaagcg agtcgaagga ttgaccgttg aagaataaaa gatggctgac
     4141 tgagaatagc ctgagtagtt gtcatgagcc tcacgcaatc tttggataaa ttctgtttga
     4201 gcattgaagg actggattat agtctgctga ctaagcgatt cttcaatcaa ctgagtctga
     4261 attcccctcg tctctgtttg cttctggaag agatgatagg atttcttggc aataaagcgt
     4321 gaaatcacca tggacagtgg cgtcaacagc aagactaaga gagtcatgag gagatgaatt
     4381 tggagcatgg ctagaatact gaccaaaatc atcaaaacac caatgaaaaa ttggttaaaa
     4441 atcatggtca agccagctgc caactgttcg atgtccgtgg ttacacgact aaccatctct
     4501 ccactacctt gcctatctac aaaggcaatc ggtaaacgat ggagcttatg gatgattcgc
     4561 tcccgtaaat ctctggtata agagaagatt agacgattat agaggagagg attggcccat
     4621 tgtaccagag tatttcctat taccaccaag agcatctgga gaaaaatctg ccaaaaaact
     4681 ggtgatgaac cagccactag gacttggtca atgacctgcc caatcagaat aggtaggtaa
     4741 attgataagc caacttgggc aatagttcct agaaaggcta ggaaaaggag gaaagaatgg
     4801 cttgctaaat ctacggctaa acgtttgagc gtctggttta cagtttgtcg tttcattcta
     4861 gtcctccttt ccatgttggg atgcattgat ttcacaatag acttggctgg atttcatcaa
     4921 gtcatcgtgc ttgccaacag ctagcaactc acctttttcc aagaggagaa tctggtccgc
     4981 catctgtaaa gttgaggttc gttgagagat caaaattaag ctcgtgtttg gaaaattttc
     5041 tctaatagct ttcaagagct tggactctgt aatggtatcc agtgccgagg ttgcatcatc
     5101 taggatgaga aacggagcct ggcgcaagac tgctcgggcg atagacaatc tttgtttttg
     5161 tccacctgag aaatttcgcc cccctgcctc aactagagca tccaagagtc cttccttttc
     5221 actgacaaaa tccttagctt gcgcaatctc caaggcctgc cagagttcct ggtcagatac
     5281 ttcttgattg aaacctagag tcaagttgga acgaatggtt cctttaaaga gttcgacctt
     5341 ttgaggtaca taggcaatcc aagaccgcca ctgctccaaa ttaagaggac tacgtccatt
     5401 ttgataaagg tcaatgttcc ccttgtctac tggataaagt ccaagtaaga gttgcaccaa
     5461 gcttgattta ccagaaccag ttcccccgat gatacctaga atttgtcctt gagtcatatc
     5521 aaaggaaatg tatctcagag aaggctgggc cgcatcagga taggtaaagg tcaattcttg
     5581 gacttgtaaa accttatctc tggtagcttg cttttgttct aactctgaat ggatatcctc
     5641 tggagcctca acaaagactt cctcgattcg cttgactgag atataggact ggttgaggga
     5701 attgatcaac atggctagct tgaccaattc caccaaaatc tgtaagaggt aattgataag
     5761 agcaatgaga gcaccttgac tgagcactcc tccttgaatt gaaatatagc cttgccagat
     5821 aataacgaga agagttccat tgacaatcag ataggtcaga ggtgttaata aactagacca
     5881 gaaacctgtc ttttcttgta atctagcata aacttggtta agggtttgaa aaatctgtaa
     5941 ctctcgtttt tcttgaccaa aagcacgaat aacccgcatc ccttgcaatt gctggcgcgt
     6001 ttcctgaacc agttggtccg ttttctttct gagactactg tagaaaggat tgaccaatcg
     6061 agataaccct acaatgacaa tggtcaaaat ggcaaccaag actaagaacc agaaagtcaa
     6121 ctcagctgag attcgataag ccataaaaat ggcaccaaaa acgataatgg gcgctcgtaa
     6181 aaagagacgc aggaattgat tgataccagt ctgaatctgg taggtatccg aagtcaagcg
     6241 agtgaccaaa ctagaagttg tcagacggtc tctgctgtcc ttgggcaagg aaagaatatg
     6301 acgataaaga tcgtttgtca attccttagc agaacctact gctgcctttg ctgagtaaaa
     6361 ttgagctatc aaggccacta aaacgccaat tactgcaaag ataaggagca ggccaatctg
     6421 catccagaga tgaccttgat ctccctgagg taaagattgg tcaacaatcc cagcaatcac
     6481 catgggaacc aagagctcaa aaacagcttc taacagcttg aacaaggggg ctaaaattga
     6541 ttccttgatg tagggtttga agtaagataa taggtgtttc ataaatccct tctattcata
     6601 ttctagaaat gaagaaagtg ggaagcccca ctctctgttt tatttgttta agtaaggcaa
     6661 tagatagcca tatcctgctt tttccatctc atccttggcc acaaagcgta aagaagcaga
     6721 attgatacag taacggaggc cgcctaactc ccgcggtcca tctgtgaaaa catgacccaa
     6781 gtgagcactg cctgaacgag aacgaacttc aattcgctcc attccatggc tcagatcctt
     6841 gtaataatga atcaactctt tggaaatcgg acggctaaaa cttggccaac cacaacctga
     6901 agcaaactta tccttggcaa aaaagagtgg ctcacctgtc gtaatatcta cataaatccc
     6961 ctcttcaaag gtttggtcat aggcattggt aaatggagcc tctgtagcag cttcttgtgt
     7021 gacacgataa gactcttcag atagactggc cttcaacacc tcttgactag gcttttcata
     7081 gtttgctgca tcaatcaatg gcttatcagc atcggtcaca tcgatatgac agtaacctga
     7141 aggattcttc ctgagatagt cttggtggta gtcttcagcc agaatgtagt ggcgtaattg
     7201 ctccacttct actgcaatct ttcgacccag catgcgttcc tgctcctgca ccactgtgta
     7261 gatagctggc aaatctgctt catcctgata ataaatccca gttcgatatt ggcgaccacg
     7321 gtcattccct tgttgattga tagatagagg atcgataact cggaaataat aaagtaaaat
     7381 ctctctgagt gacacttcct tctcatcgta aatcacttgg accgtttctg catggtctgt
     7441 ttccttgagc aactggtaat tggtcgtttc gacttgacca ttagcgtagc caacactggt
     7501 ttctagcact ccagaaatgc gtgaaaaata ttcctctagg ccccaaaaac aaccacctgc
     7561 tagataaatt tctgccatct ttatttctcc tttatcacgt ttttaactaa tactcttcga
     7621 aaatctcttc aaaccacgtc agcgtcacct taccgtaggt atggttactg acttcgtcag
     7681 ttctatctgc aacctcaaaa cagtgtcttg agctgacttc gtcagttcta tctgcaacct
     7741 caaaacactg ttttgagcag cctgcggcta gcttcctagt ttgctctttg attttcattg
     7801 agtataaact tcttttcaca aaaaggatat gaacccctct ggtcaagagt ttccccatcc
     7861 tatcttctat tctttatttt gcttctacac ggacaccttg ggtatcaact ctcaagtcat
     7921 gcagttttcc tttgaaaggt tgcttttcca attctgcctt aattgttggc atcttgtcat
     7981 gagaagccag aaccataact gtcggcccag caccagaaag gtaggttgca taggccccat
     8041 tttctttggt cacttgctta atcatcgcaa attctcttac caagtcctga cgatagcgct
     8101 catggaagag gtctccctcg attgcttgcc cagcggtcac catgtctcct gccaacaagg
     8161 cagcaaccgc tacattggcg atagaacttg cagcaacagc ttccttataa gacaattttt
     8221 taggcaagac actacggctg tcgcgagtac gtaattcata gtttggaatg taagctagaa
     8281 aatcacactc tggaaagtct gctacgatag cagagacttg cccttcaaca gaacttgcaa
     8341 taacgagatt accataaatg gctggagcca cattgtcagg atgcccttca atcttggtcg
     8401 ctaactgcaa tttttcatgg tctgataagt tgagttgacc cagttggttg gctagttcaa
     8461 tcccagcaac gataaccgag ctggaagaac ccaaaccgcg cgccaaaggg acatcactgg
     8521 tcattttcaa gcgtcttggt tgcaagtctg gtacaatttg caaagcgatt ttgagcaaga
     8581 gattacgctc gtcatgtgga atccatttgc caatctggtg ttcaatcagc cactcatctc
     8641 gttcttcgca gacctcaatt tgaagatact tggttacagc tacaccgacc gagtcaaaac
     8701 ctggcccgat attggcactg gttgcaggta caataatctt catcttattc tcctagcacc
     8761 ttgaaggtat tcaagaggtc gaattctgaa accttcttca attcagctga gacattttca
     8821 agctgggctt tattaatctt gtgtgtgatg ataacgacac gcgccttgtc accctctttg
     8881 ccatcttgaa ggatttgctt aaaggaaata tcttgagcat tgaagatttc agccaacttc
     8941 aagacctgac cttttgagtc tagagccaag attgagaaat agtagtttgc tttgacatct
     9001 tcaggatttg ccaagaccaa gtcacggcta tattcgttga agtctttgcc aatagtacca
     9061 tcattcaaac gacgaacgat acggacaata tcagctacaa cacttgttgc agttggtttt
     9121 tgacccgcac ctggtccgta gtacatagac tcaccaatac cgatagattc tacaaagaca
     9181 gcgttcatta cgccattcac actagcaagt gggtgcgctt taggtaggaa ggttggagtc
     9241 acttctgcag caatacctga agaagtttcc tcaatagaac caaccaattt cactacgtaa
     9301 ccaagctctt gagctacagc tacgtcttct ggtgtgatat tgcggattcc cttgtgggct
     9361 acatcatcaa aggcaatctt catgccaaag gcaaattggc tcaaaataac catcttgtag
     9421 gctgcatcaa tcccatctac gtcattcgtc ggatcgcttt ctgcaaatcc tagacgttgt
     9481 gcttccgcaa gagcatcatc gtaagaccag ccttcttcca ccatcttggt caccatgaag
     9541 ttggaagttc cgttgactac tccaagcacg cgcgtaattt tatcagaagc caaggaattt
     9601 gctaaagtac gaagaattgg aatcccacca gcaactgctg cttcgtagta aagtgctacc
     9661 ttgttagctt gagcgatttc tagcaattct gcgccatgga cagctaaaag gtccttgtta
     9721 gcagtaacaa cgtgttttcc agcttccaag gcacgagtga taaaggtttt agcaggctca
     9781 atacgcccca tcaattccac tacgatagta atatcctggt ctgataaaat atcatccaca
     9841 ttggttacaa agttaaagtc attccctgct gcaagcaagc gatttttttc atcttcatcc
     9901 ttgaccaata ccttagcaac tttgatatct gaatgtgctg attgattgat ttttcctcca
     9961 ttttccttta ggaggaaagg cacaccactt gcaacggtac caaatcctag taaagcaatt
    10021 ttaactgtca tctttatctc ctcgaaattt tctaatatag ccattataac agaattttgt
    10081 gaaaattcct attatagtaa atcactattt cagtataaaa agaaaaaacg aatcagacga
    10141 ttcgctcttc ttaaaatctg aaaatagctt tccagaaagg attagccgat tttttgcaga
    10201 ttgagcactg catcgtgact catcaagact tgaccatact cttgtaagac tgagcgactg
    10261 atatcactat cgtctgcaaa ctcgcgcata cgggccaaca gccaagctgg atatgggctt
    10321 ggatgatttt caatatccac taaaatggtc aaataatagc gctcgttcat tttgtagagt
    10381 tcagaagttt ccatttcaaa agtcactgtc ttggcaaaag ctaccaagtc agccaactta
    10441 gcaaaagaaa ggatgtagta gatgtaaggt tctttcttac tctcagcttc ttgttcagcc
    10501 tgctcttgct cttcttcctt gacttcaact tgctcaagag attgaatggc ttcgatatca
    10561 tccttggttt tgtctgcgat gcttttttcc agggttttga taaattcatc tggagacatt
    10621 tgagccaatt cttccatatc tggcaaatcc gataagtctt caaaatctag attttggtca
    10681 atctttgact tggtcacaaa gacatctacc ttatcaggtt ttggagtcac acggaagctc
    10741 aacatgcctg tatccagaaa gctatcaggc atctctagct catccaagat agcataaaag
    10801 aactcttctg ttttttcttg aggaacgaga aagtcagcaa tctccattcc acgatccatc
    10861 aaatcctcta aagatatcgt gatttttaaa gttgtatcac taatttgttt cattttcatt
    10921 gctagtaacc tcatactttc agttctatct attatactag atttttacga ttttatcaaa
    10981 agaaggctcc tctatacgga tagattttcc ctagggtctt tctataggag actccaaaag
    11041 aaaatttctg cagacagata gaaaaagcct tcaaaatcgg ctaagagccg actttgaaga
    11101 ccttatacat cagaatactt ataatttaaa ggttgctaca ccgaggatag aacgatttaa
    11161 gtttctgaga atttgaagac tttgctcaaa tttcttataa cgagtcactc cgtactcttc
    11221 aacaagaagg actgtatctc tttccaaaag agatgataca tcctgtaaat ctacaaaatg
    11281 cattcctttt aaagcttctt gactctgttt caatttatct aagatagctt tatttgagct
    11341 aacgatggtc aattcctgtc cagtattttt gtatgacaaa acatctgcta ggttagcaat
    11401 tgttgtaatc tctgttacaa aatcaatttg atactgagaa aaatcaccta ctctattgat
    11461 tgttggatta aagagataaa ctaacacatt tcccatcaca accaaaatca cacaaaccac
    11521 tccaataaca actaaacgaa gaatcagatt tttcacattt aagccaagcg ctgtttcacc
    11581 atttgcgttc aattctttag agttgatggt ttccagtttt tcaattttca catttgcata
    11641 ggcatgttta aatttctcaa tcaacccatc aatttttttc tctaacaagt tattggcatc
    11701 tttacttgat gtcaaaattt tcacaccaac ccctgcatcg tcaatcatat agtagacggt
    11761 caattttttc caccaatagt cattcgttga atttttcaag gttgtttctg tcgtgtctaa
    11821 ttcactggca atttttttca actcactggg ttctacatca ttgaaaagat aagctccatt
    11881 caaattacca tcaatcaatt tcccataaaa atcactataa ccaccaattt gatgattcaa
    11941 aatcgttttg tccgactctt ttggaggagt gattttatag ataagataag ttgaataact
    12001 tgttgtatct ttgacagtgt ttttattcct aactgcttta attgtaaatg gtacagcaat
    12061 gagagcaaat aaagcgatga gagctaaaat atttgctttt cgctttttat aaagatttgc
    12121 aaacaaatca gctactgaat aatgttcaaa catgattttt ttctcctttg tttagtagat
    12181 actagttttc ctttgtaagc atttttgcta caaatataat cacaagaaca attccccaga
    12241 attgcattgt aaataaattg aagaaacttt ctgaaaagct gcttcttggc ataaagaata
    12301 gattattcaa gatgagtagg gataaagcaa ataggattgt ccttgagcga taggctactt
    12361 gcagcatggc tataaataat acgccgagta agaaactaag cagaaagact ccaatcatac
    12421 catagtcggt atacaactcc atgatataac tacttccgat accatgccct ttcaagtatt
    12481 ccttgttcaa gacaagatag gatagattgt gggcataact attactatca atagctagtt
    12541 ccacactatt ggttgtatgt tcaaaggctt ttcctccgaa aatggctccc aaactccccc
    12601 ttgcaaaata atcaagaaca ggaccaaaag taaaattacg gaaatctcgg taagggaggc
    12661 tactgttaaa tagaaaacct cgagccagaa caccaaaact agtcccttgt ttatagataa
    12721 agtcaagtaa gatatcccag aaacctgtat gggaaacttg gacattatcc cgtacataat
    12781 tgagtactcc catcgctaac atgagaatag gagaacctac aaaaatcgct aacttttctt
    12841 taaacccaat ccattttcct ttttcagttt gctcccgcat aaagtaataa acaaaagcaa
    12901 ataaaatact taaaataaag ggatttcgtg tcccaattgc caaatgaata gtattagctg
    12961 caataaagga gacaagcact gctgtggcct gcaatttctt tggcttggtt gccagataca
    13021 tacacattgc atagaccgta aaggtagaca aaatgtaggt aaaataaggc agtttacttt
    13081 caaaatttgc atagtaggca tagtaggaag tctgcaaacg atacaagagc cgttcaaata
    13141 accgaatgaa atagaaagga taagttagaa gaaaaactcc tagtgataca aagcgtaacc
    13201 gcttgatata aacctctttt agagaatttc ctatatttgc tacttttatt ttcttcctag
    13261 ctatgaagta acgagccaga atgcctcctg tggtcaagcc cagaatcgaa atcatgacaa
    13321 ctataaaggc aaaacgatag gctattggat gataggtatc caaagcacca tccctaaaat
    13381 aatcaatggt cggtcttgat accagaaata caaaaatggt taaatagaaa ataaaatgga
    13441 ttaagtaata cttgatatca ttccaacaag caattaagct actaaccaac aagaacaata
    13501 aagtagaaag taagctaaca ttattattat taaacagata cacaattcca cttactagcg
    13561 tcaaggcata actgactatg gtcaaactaa ataataatcg tttcccatca atcacttggt
    13621 cacccccgtt ctaatgtaat tttttagatt tttcaatatt tttcagtaat aagaatcgat
    13681 ataaggaaat atttatgaat agggccaaag cactaattct tctcccctta cggaaaattg
    13741 gattcctaga aatagcaaag gcatggcctt ttaaaaaacg atgaatctga gaataggctt
    13801 caaactgttt atactgatca tctagcaaca tcttatccag aataaagaag tgggcatagg
    13861 ccaatctgaa aaaagcgacc tctttcaagt caggatagtt tttcacaact tcattataaa
    13921 acttttggta gatatcaata taggctaaat ccttctctgc atagggtttg gtcgtaatac
    13981 tatcccctct atggaaatag taataatagg gtttagtatt aaccacatac ttcttggcca
    14041 acttgattaa atcaaaatgg taataggcat cttcgtaaat caacccctta ggaaaggata
    14101 gggcagttgc aatctgtctc ttgattagct tattgcaaat cgtcccaggt attttttcac
    14161 ctatgaggta ttcctttaga aatgtttgag aatcacagac aaaatagtca tcctgattgg
    14221 ctgactgtgg gctttcatca ttagcataga cattcatgac accacagctc gaaacatccg
    14281 catcttcttg aactaattgc tcatataagc tctgaatcat ttctggatgg atataatcat
    14341 ctgagtcaat aaaaatcaga taatccccgt gagcctgctt catcccatca tttcgtgctt
    14401 gcgacaatcc ttcgttcttt ttatgaagca ctgacaccct gtcatcttgt tcagcgattg
    14461 aatcacacaa gcgaccactt tcatctgttg caccatcatc aacaagaata atttccagat
    14521 tttgataggt ctgcttctga atggaagcta tcgatttttc taggtactgc gccacattat
    14581 agactggcac aatcacacta attaatgcag tttccatgct actcctctaa tagtttttct
    14641 acttgttcga tttgttttgt aattgtaaat tgttgaatga attggctagc ctcatcgaca
    14701 tcaaagtttg aggcagaagt catgtaatta gtaatcgcct gagctgcctc ttgattgctc
    14761 tcaatgattt gtccaaatcg tccttcttgg gataattcct cagcccctcc aacgtccgta
    14821 gagataaaag ggagtcccag actcaaggcc tccacataca ctccaggaaa accttcttgt
    14881 ttagacatag acaaaagaac tttcgtctga gatagatact gataaggatt tttttgataa
    14941 ccaaggaaat gtacatagtc ctcaatccca tactctttga ctcgtttttt cagttcctct
    15001 tccatatcac cagccccgat aaaatagaga tgatagtttt ttccctcttg gtgtaataat
    15061 cgtatcactt ccactacacg gtcagaaccc ttattttcct caatccgtcc gatagtacag
    15121 atactttgag gagcaatctc gatatcgatc ttctcttgag atttttctag aatagtctga
    15181 aaatcatatc cattgtagat tgtctgtaat ttagaagtat aatctggata aacttccttg
    15241 atagaattgc tggtcttttt tgaaatccct acaattgtat tcgcagcatc caactggctt
    15301 ctatgtgatt ctcttttaga gctatcctta agaagttctt caatacttcc atgaatccaa
    15361 gatatcttct tgacttctct tcttttagag aacaacagtg gtggattcat aatggtaaaa
    15421 gaaacttcaa catcataatc atcttttaca agcaaacgac gagtcagtct tggaaaataa
    15481 attctcattc tccacaaaaa agctcgtaac catctggttt ggcgataatc ttgaagggat
    15541 tttaaaatgc gtacatgctt tggaacagat tcatatccct tgtcaaagtg ctccatttca
    15601 agaatatcaa tatcatactt ttctggatcc agatttgaaa caatggttga tagaatcttc
    15661 tctgcaccac ctccaagaga aaaagaccac ataaaaaata agattttttt cttagccacc
    15721 atattctccc ttgtattctg tataagactt atccatatca gcgatgacag catcatgatg
    15781 cggtacctgc ttgtctgctg gtggaggcgt catataatcc ccaaaagcag ttctgagata
    15841 gacatcatag ccgattggaa taggcatctc tgttccttca aatggcaaga aaagattgtc
    15901 ttcaaaagat gtgattgggt acttgtttct catgtagcca ggacctgagc ataattctgt
    15961 aatgccatca caatcagcca aatcatactt agtcatttct ttctcagctt ttttccagat
    16021 gcgataacgg agagattttg gagtcaaacc cagtaaaatg cgacttcccc atttcatgag
    16081 atcaccatgc ttttctggaa tagtttgcgc acaaaagagt gaataaatca aggcccaacg
    16141 aacctgtttt ttccgctcag ctggattttt cggataataa tccaaaggca aaacatccaa
    16201 ggccagacca tgtggcaaat ccaaatcctg ctgataaggc ttgatacagg tggttttctt
    16261 gtcacgaatg gtaataaaaa gattacgatc aacaaaatcc ttgtgactct ttgacaagaa
    16321 ataacgttca tctgcataac gaggccataa ttctgctaat ttctcataat ctttacgagg
    16381 cataaaaaag tctaggtcgt cgtcccaagg aataaatccc ttgtttcgaa gggcaccaat
    16441 agcgcctccg ccacagagat aacagagcaa atcatgttct ttacaaaagg ccacaaaata
    16501 ttcagccatc tccagactac gagcctgaat tgcttttaaa tcagtcatat tgttcattat
    16561 tctttctatc gtatcgtttc attataccac aaacaagggg tgaaaatcta ttgcagactg
    16621 taaaaaatca aagcctgact gctatccaaa tagctatcaa actttgattt ttctgtctta
    16681 tactcttcga aaatctcttc aaaccacgtc agcttcacct tgccgtaggt ataggtaact
    16741 gacttcgtca gtcttatcta caacctcaaa actgtgtttt tagcagcctg cggctagctt
    16801 cctagtttgc actttgattt tcattgagta ttatcttatc ttaagcccat ttgagcgagc
    16861 ttggtttgat atttgttttg atcaaccagc aggcccaagc ccccataaac atcataggca
    16921 tctacccagt cacccagttc tggaatcgtc aatttttcaa taccattttt tgctccatcc
    16981 aaaacagata aaccgtttgt taggaggaaa gtatagggta cgttggttga ggtcatagca
    17041 aaaacctttc caagagcttc agaaccagtg aaaagtttag tgggatcttt aatttgctct
    17101 aaaattgctg ttaaaacttg ttgctgtctt tttgtacggc cgtaatctgc ctcatcatca
    17161 tcacggaaac gagcataatt gagcagggtc gagccattca tctgctgttt tccgacttta
    17221 atggtttggg ttggagactc agtctcggta gcgtataaat catctccgac tgtagcttct
    17281 gttaggggac gcccattcaa tgttgaaaat tgagcatcaa tcgtcacccc atcagggaaa
    17341 agcgtgtcaa tcgctgtggc aaaggcctgg aaatcaacca aggcgtagta cttaatgtcc
    17401 aagtcaaaat tatctttcaa gacttggcga accatttctg cccctttttg cccctcttgt
    17461 tctcctaact cgtaggctac gtttaacttg ttatctgtct gttttctacc attaatcact
    17521 tgactataac catctatata gaccaaatta tcacgcatga aactgactag cttcattttc
    17581 ttatctgagc ccccgacatt taataccata atagagtcag ttcgtgtctc aacactgttc
    17641 tggccgattc gaccatcagt acccatgatt aaaatattaa ctccatctct agtgtcctga
    17701 ccattaaaga cttctacttg agctgcccgg gcatcagcag ttttctttgc gctagcatct
    17761 tggtaaccac gcaaaaacat gaataccatg gccaaagcca cacagaccaa aagtgaaaaa
    17821 atcaccataa aaattcgttt aagacggagc ttccgtcttt tcttttttgg agggaaagag
    17881 agtgcttgtg atttggattg tgagcgactc cggttcgcat agcttggtaa gtcaacctgc
    17941 tcttctcttt cttgttccaa gctagagcta ctatttcccc tagcaagagt tagcttttct
    18001 tgcaaatagg caaactcatt tttttctctc tcattgagat agtgaatatt ttttagcaaa
    18061 taatcataac gcaactgctc atgatgactt aagggatttt ctttactcat cttctctcct
    18121 ttccatggtc tgatattgga taaataggat aggcacccag aattttatac tggattccaa
    18181 tcgcttctaa ttctttttgg gcaaagtgga ccaagtcctt atcggtataa tccacatcga
    18241 taatgaaaaa gtattcaccc agtgctgtct tgagtggaca actttcaatt tttgtcaagt
    18301 caattcctcg ccaagcaaag gtcgacaggg ccttataaag tgcacctgga aggttgtcag
    18361 gtaatgtcaa ggccaaactc atcttttcag tttgtgcttg caagggaata ctaggctttt
    18421 cagctcctag aacccagaaa cgtgtgaaat tggcttccat ttcctgaata tcctcggcaa
    18481 tcagttccaa tccatattct tcagcagaac ttctaggtgc aactgctgca aagggctggt
    18541 ctggatgttc ggaaataaaa cgggccgcat aagctgtact agctgttacc tcgatttgag
    18601 cctctggata ttgttcatcg atgaatttct ttccttgagc caaggcctgt ggatgtgaaa
    18661 aaatcttttc aatcttagta tggcctggaa ccaccatcaa ctgctgatga ataggctgaa
    18721 cgatttctgc tactgcttgg atgtgagcct gatgaaaaag atagtccaag gtttcatgaa
    18781 cactaccctc aatagaattt tcaactggca ccacagaata gttcactaat ccttgctcat
    18841 aagccttgat gacatctgta atgttggcaa aagcctgcaa ttcctcatga ggaaaagctg
    18901 tctgcacaac gtggtgtgaa aatgatccct tgggacctag ataagcaatt ttcatcttag
    18961 ttcctctata atttcctctg ggcttagctt ggtcacatcc aaaacccgac tagccacttc
    19021 ctcataccaa gcctgtcttt cttggaaaat agctactagt tcttccttgc tattatttag
    19081 aaaaagcggt cgctgattgt ccttatcagc tgcgatacgt tggtagaggg tttcaaaatc
    19141 tgctctcagg tagatgttat ctgtattagt cttgagtaag tcacgatttc tctgagaaat
    19201 aaccactcct cctccagttg acacgacttg gtctgtttgt agtaaatcag ctaggacttc
    19261 tgattctacc tgacgaaagg ctgtttctcc cttttcagcg aaaaaattcg caatggacat
    19321 acctaggcga ttctcaatca gagcatccat atcaaggtaa ttagggtcca agcctcttgc
    19381 aatagtcgat tttccagccc ccataaaccc taataacacc ttagccatga atcaagctct
    19441 ccaaatcatc aaagaaacta ggatagctgg tattgatggc ttctgcacgg tcaagctcca
    19501 cctctccatc tgcaaccaag agggctgcga tagctgtcat catgccgata cggtggtcac
    19561 caaacgtatt gactctagca ccgtgaagag ctgattttcc tttgataatc atcccatctg
    19621 ccgtaggagt aatatctgct cccatactat ttaaggcgtc tgccacaacc tgaatacggt
    19681 ctgtttcctt gaccttgagc tcctcagcat ccttgataac tgttacacct tgggcttggg
    19741 tcgcaagcag ggcaataatg ggcaattcat caatcaatcg tggaatcaaa gcgccaccaa
    19801 tctctgttcc tttcaagtca gaagactcaa caatcaaggt agcagattta gcgactggat
    19861 cgatttcagt tatttccaat tttccaccca tggcacgaat gacatcaata ataccggtgc
    19921 gagtttcgtt gatccccaca ttctgcagca ctagacgaga atttggagca atcaaacctg
    19981 cgactaacca aaaggctgca ctggaaatat ctcctggtac gaccaccttc tgtcctgtca
    20041 atttttgtgg cccctggact gtgattttct taccatccac acttaaatga ccaccaaatt
    20101 gtttcaacat atcttcagta tgattacggg tgtactcttt ttcgataata actgactccc
    20161 ccttagcttg taaggctgca aacatcaagg ctgacttgac ttgggcagag gcaattggca
    20221 actcataatg aataggtctt aggtttttcg tcccttttaa gcgaagggga ggcaagtctc
    20281 gttcagtttg ccctgaaatg ctgacgccca tttttttcag tggaagggtc acacggtcca
    20341 taggacgttt ggaaagacta tcatctccaa acatctctac ttcgaaatct gcaccagcaa
    20401 ggacacctga aatcaggcga atcgaggtgc cagaatttcc catattaagg gcattttgtg
    20461 gcgcttttaa gccagccatg cctacacctt gaatggtaat aaccccatct ttatcctcaa
    20521 tttcaacacc aaggtcacga aaaacctgca tggtcgaaag aacgtcttca cctcgcagaa
    20581 tatcataaac cttggtctca ccctcagcca aacttccaaa gataatggaa cggtggctga
    20641 tagacttgtc acctgggacg cggatactac catgtaaatg gcgaatgttt gtttttagtt
    20701 tcatactgga cctcatactt gcaatacttt tacctatttt atcataaaaa gccagaaatt
    20761 ccttaaaaat tcctgacttt aggatcgttc ttttcttatt tcagcaattc tgaaactggt
    20821 tcaaaaacaa ttttttcaat atcagaaagg taaatggcca attgttgttg cttggtaaag
    20881 aattctgaca agaggctatt tccttgaatc tgtttaccaa agccttccat cttagcttgg
    20941 aaggacgcat ctggcatttg acctgtctgt gctagttttt gaatttcctc ttgaaaggca
    21001 agataatctg taaagatttt gcttgcctca gcatctgctg caatcgcatc tttagctgct
    21061 ttaacagcct tgtattctgg taatccgcgt agaccgcgac tgagttcgtt tgcactatcg
    21121 taaatatttg acatgttctt ctccttattt gatgacgact gtatagtcag tattttctgt
    21181 tatgagatgc tcagctcttt ccaagtcttg agcattttta aatgaaattt gtaggattcc
    21241 gtgaatatcc tcacgatttt cctcgttgat gtggatatta accaaggaag ttccacgtag
    21301 cagttccaaa atccgcagga tgacatcttc ttcatcagga acgtcaacat agaggtcgta
    21361 agagctatcc acaccaccac gcttatggat ttccatggtc tggcgttgtt cacgcgcttg
    21421 gttaaaaaag ttccaaattt gctcttcatc tcccttacta atggcctgac caatcgcttc
    21481 caaacgttcc ttgaaatcct caattctatc cagaatgatc tcgctattgg acaagagaat
    21541 ggaggtccac attcctggct cgctttccgc aattcgggtc atatctcgaa aaccacctgc
    21601 cgcaaagcgc cttgccatct catgctcttg agcatagacc gcagtctgct ccatgagact
    21661 agaagccaaa atatgaggaa aatggctaat ctgagaagtg acacgatcat gctccttggc
    21721 atcaatctcg ataaaacgag catgaagacc tgaaagcaga tccttcattt ccttaagcgt
    21781 gtcctgactt gtcaggcttg aaggtgtaaa gatataatag gcattttcaa aaagattgac
    21841 atctgccgaa gcagcccctg tcttgtgact accagccatg ggatgggccc cgacaaagcg
    21901 aacagacttg ccagccaaat actgctccgc cgcatccaca atggttgact tggtcgaacc
    21961 agcatctgaa ataataacgc cttctcgcaa atccaaattg gccaactcct taatgaaagc
    22021 aatagtttgt ttgattggca agctgaggat aatgacatct gccaaaggag caaaactagc
    22081 aaaatcatcc gttgcacggt caatcatacc ttctttcaag gcgatatctc tcgaagcttg
    22141 actacgatta taacctaaaa tttcataatc tggatgatcg cgtttgatac caagtgccat
    22201 agaggctcca atcaacccaa gacctgcgat atagattgtt tttgccatag gaactcctta
    22261 atagttcttt gtatagtctc ggtgtttggc taccgcttct tttagttcct caagattatc
    22321 tgatgagaat ttttcgagga tttcttgcgc cagaaccgtt gctacaactg cttccatgac
    22381 cattcctgca gctggaagag cagtcggatc acttctctcc acggttgcct tgtaaggttc
    22441 gtgggtttcg atatccacac tcataagagg tttataaaga gtaggaatgg gtttcatgac
    22501 cccacgaaca acgatgggtt gcccattagt cataccacct tcaaaaccac ctagattatt
    22561 ggtacggcga gtataaccgt cttctttaga ccagagaatt tcatccataa cttggctgcc
    22621 tttacgataa ccagcctcaa agccaagacc aaattccacc cctttaaagg cattgataga
    22681 gacaacagct tgagccaatc ttgcatccaa ttttctatcc cattggacat aggaaccaag
    22741 accaactgga acgcctccga cgactgtctc cacaacccca ccgatggtat caccatcacg
    22801 tttgatttgg tcaatatagt ccttgatttc ctgttctcgt tcttggttga caatagaaac
    22861 ttcagactgg gcagctcttt gcttaatttc agcgactgtc agattttcag gaacatcgat
    22921 ttccttgcca ccaaagacca cgacatggtt ggcaatctcc atatccagct cagccaagag
    22981 gcgtttggct actgcaccaa ctgccacccg catggtggtt tcacgagctg atgaacgctc
    23041 caaagaattt cgcaaatcat caaaacggta cttaatcccc ccaaccaaat cggcatgacc
    23101 tgggcgagga tgagtaattt tccgcttgct tttaaggcgg tcttcaatgt cctccgcaga
    23161 catgatgtcc agccatttct ggtggtcctt attgatgaca tccatagtaa taggcgcccc
    23221 tgtcgtcttc ccgtggcgaa cgcccgaagt aaagacaacc tggtcattct caatcttcat
    23281 acgaccacca cgaccgtagc caccctgacg gcgtctaagg tcctcattga tatcctcagc
    23341 tgtcaatgga agtccagctg gaattccctc aataatagct gttagacggg ggccgtgtga
    23401 ttctcctgca gttaaatatc tcatacactc tccttatttt accaagtagt ctttcatctc
    23461 ttccagagaa actgggtgaa tggtcgctga accaagctct ggcaccaaga ccaatttcaa
    23521 ggtgttacca cgcgctttct tgtcatgagt aagagcctga taaagcttgc caacttccca
    23581 attttcatag tcaacaggca aaccgaattt ctgacacatc tctgtgatag attgggtaat
    23641 gccagctggc atgaggcctt tttcctcagc aaccttggaa atctgtacca ttcccatggc
    23701 aacagcctct ccatgcatga ccttgccata accggcagtc gcttcgatgg catggccaat
    23761 agtgtggcca aaattgaggt aaagacgaat accattgtcc aactcatctt caaccaccat
    23821 cttgcgcttc acctgacaag aatgttcaat caaggtctct gcatgttcca aaatactctc
    23881 aacagaacca ttcagtcccg tcaagagagc ccacagttct ggatcctcaa tcaagccata
    23941 cttgataact tcacccatcc cttcaatcaa ctctcttttt ccgagggttt caagaacaag
    24001 tggatcaatc agaaccccat ctggttgggc aaaggtcccc accatatttt tagcaaatgg
    24061 tgtattaacg cctgtctttc caccgataga agaatcaacc tgagctgtca aactagtcgg
    24121 aatctgaaca aagtgaatac cccgcatata ggtagaggct acaaatccag ccaggtcccc
    24181 aacaacgcca ccaccaagag caacgattcc atcgctacga gtcagacctt gcttgactag
    24241 aaattcatag actttctgaa cagtagttaa attctttctt tcttcacctt ctaagaaatc
    24301 aaaaacagct acctgaaaac cagcatcttc taggctgagc ttgaccttct ctgcatagag
    24361 agaggctaca tggttatctg tcacaatgac taccttttgc ggttgccaga gttctcgcaa
    24421 ccactgacca gcctgggcca tacaaccttt ttcaatctga atatcataag gatggtgagg
    24481 aatatcgatt ctgattttca taggagagtc tccctttctt tattggtatt tttctgttaa
    24541 agactgccaa atctcttctg tcggcatttc cttgcctgtc cacagttgaa aagcttctgc
    24601 agcttgatag agtaacattc ccagaccatt gactgctgga ttgccctgac ttctagccca
    24661 tttcaaaaac ggtgtttcaa agggttggta tatgatatct gcaactaaaa gagtttctgg
    24721 taagactatg ttttcaggaa caggagagga ttggccatcc atgcccacac tggtggcatt
    24781 aactagcaaa tccgactcgg caatccttgc ttgcagttca gaaacatatt ctaaagcaca
    24841 caaatccact ttaaaacctg tctgctcctg taacttgtct aggtaaggtc ttgttttttc
    24901 catagaaacg gaacgaacaa agaccgaaat ctgactgacg ccatccaaaa tagcctgtgc
    24961 caagattgat ttagccgcac cacctgcacc cagcagggtc atctttttac ctgaaattgt
    25021 aaaagaaggc aagcacttaa aaaatccctt gccatctgta ttatatccaa ttaaattgcc
    25081 attctcattg acaaccgtat taaccgcacc aatcaagcgc gcttcatcgc tcagcttatc
    25141 caaataagga atcacctgct ccttataggg catggacaga ttgatgccaa acatctggta
    25201 gcgacgaata ttggccactg tttctaccaa gtcactcgct tcaatctccc aagccacata
    25261 agcaccgttg gtagctgtcg cctcaaaggc tctattgtgg atgaagggag aaatagaatg
    25321 cttaatagga ttggcaacaa ctgcagctaa acgtgtatag ccatcaagct tcatccaaaa
    25381 tctccctgat ttttttcatg ctagctagag aaatctgccc aggggcacta acctcatcca
    25441 gactggcaaa agaccaactc gaaccagtca catccgcagt gatacgagag accttgccca
    25501 ccttacccat agaaatggtc acatattcct gttcaggatt gagggtttta aagcctcgtg
    25561 tatagttcat caagtctaag acatcctgct ccgtgtgagc catcaccgca accttaacaa
    25621 gttttggatt taggatcgtc aactctgaca agatttccat catgttctca ggtgtttctt
    25681 ggaaattatg gtaactcaaa acaagatttg ggaagtccag catttcctca aaaacatcct
    25741 tgtagctata gtactcaaaa tcaatatagt ctggttgata gagttgcgca acttccttga
    25801 ttagatggat atactcttct ggagaaaggt cgatttctcc accttcggag cgagttcgta
    25861 gcgtgaaaac caactcacgg cctgcgaatt tttcaaaaat ggctggagct acctgcaaaa
    25921 tcgcttcttt aggcagatag tcggcacgcc attcaatgat gtcggcatcc aggtacctcg
    25981 tggcatccag agcctgagcc tcctctaaac ttcttggcat tactgaaacg attaatttca
    26041 tttactaacc ttcatactaa tcaccttgag gtaattacta ctttcatctt ttttattata
    26101 ggcaaaatct gctggaagac catatttgtt taaaatctgg taacttcttc ctgcaaaacc
    26161 tttatcaatt tgttctgtaa atttctgacg ggaaacattg gcagcattgg tactggcaat
    26221 gataatccct cccggattta aaatctcaag actctgggaa atcaacttgt gataatcctt
    26281 ggccacagag aaagtttgtt ttttattccg agcaaagcta ggcggatcta ggacaatcac
    26341 atcgtaggtc aagtctttgc gtttggcata tttgaaatac tcaaagacat ccatgactat
    26401 aaaacgatgc tcgtctgtgc tgagcccatt tgcctgaaaa tgcgcttgag acaattctcg
    26461 tgaacgtttg gctagatcaa cagaagttgt atggctagct cctcccatgg ccgcagctac
    26521 tgaaaaagcc gctgtgtagg aaaacatatt gagtaaggat ttacccatag ccaagccgtc
    26581 aactaaacta ccgcgaacct catgctggtc taggaaaatt cctgtcatca agccatcatt
    26641 cataaagact tgatacagga caccattttc taaaacattg aaaaagtcag gtgcttcttg
    26701 accataaaca tgggcagatt catagtccaa acccttaaag cggattttct cataagctcc
    26761 taaaacctca gggaaaacct gtctaaaggc ttctgatata gtctgacgaa tctgataaac
    26821 ataagagtta taccaagaaa agacggcgta gtcgccataa aggtccactg tcagaccccc
    26881 aaagccatct ccctcttgat taaagagacg aaaggcagtt gtcaaatcat cttgatagta
    26941 ggcgtttctc ttttctttgg cttttctaaa caacgtttca aagaaagctt gattgaaggc
    27001 caccttgtct ttgctgataa accagcccaa gcccttgttt tgctgagaaa ggtaggcagt
    27061 cccaagaaag tttccttcct gaccctgcac ctctacttcc tgatccttaa gattgacatt
    27121 ctcaagatca ctggcttcta gtaaaactag ccccttagca agcttctttt caaccctttt
    27181 gctgactctt attctattca taactaccat tatatcaaac ttttagacaa ttctcaaaaa
    27241 agaaactacc cttgcttttt tactcttctt ttaaaaaatg gtatactaga cttcctgcaa
    27301 aactaggaag taaatgtgta agaatcacag taaaaaatgc tcttccgtct tggaggagca
    27361 tttcttttta tcaacgaaaa tcaaatagca aactatgaaa ctagcctcag gttaactgtg
    27421 agattatagg tagagaggtt gtatcagcaa tatgtgtctg tcaaatttag tgacaaaggt
    27481 agtagaagaa agataaagaa ataaatcagc ttcagtaggt atctggaaaa tttgatttta
    27541 tagagaagcc ttttgttaca aactcaatat actatcaata aataatatta tagaagcaac
    27601 aataattata atttcaccta tctgcatcat tctatttcga actctaaata tatgttctat
    27661 caaaaatact tggaacacac acattatagg aattaacgtt tttgaaattg aaaaatatcc
    27721 aaataaataa actataaaca acaaaaatag aactatgtta tatttcttat tcaaaacatt
    27781 cctccctata tatttttgat taccaatctt aatcatttac aactacattc taacaaacta
    27841 taaaagcgtt tgtcgaattg aatttatcaa gcaagcgacc aaccagttca tcttttttct
    27901 atttctgcca atatgcgtga caggtaataa tgatagccaa aaatagcaag agcaagcaag
    27961 acgataagag ctcctactcc caagctgatg gcaaggatag gggagagaga ctgaaccaag
    28021 aatatgctcc caattacaag ggccatcagg attgcactat aaataaacaa taaaactatg
    28081 gcgactatgc catttgaacg attcaccagg tccgtaatgc tactccaatt ggttgacaga
    28141 tttttaacgt ccttaaagta atggtggcaa gaaaggatga cactggcaat gatccagact
    28201 acaagaaggt aaatcatcga aatgatgggc aagcctagat atagagaaag accaagcaaa
    28261 gtcagaactg gtaaaaagga ctggacagca tatataatcc aaaatttcac tttcacataa
    28321 cgagcaaagt caaagggtaa actcttaaga aaatcaacat tttccctctc caaggacaag
    28381 gcaattgaat gcaggctggt gatattgtta ttgacaactg ctataaagag agctataaaa
    28441 aacaagggta accagtatgg aggatgaatg tctggaacta tctgagaatc tcggattttg
    28501 gaaatcagac cgatcatcat gagataagga aggaaagcac ttgtaaaaag cactgtaatc
    28561 acgccagtcc cctgtcccaa gagggtgagg tggtagcgta aaaccatgcg aaaaaatccc
    28621 tttttagtgg ttgaaattct ctccttgctg cgacgttctt ttttgacctt ctcctcacta
    28681 ttaagcagga tcacgtcata aaaacgagga aggaccttct ttttggtcag ataaagcagg
    28741 aagagagtta gtcctatcca agcgagcaga cccactaagg cttctgtcga aaaaggctcc
    28801 actgctattt tgtaaaagat atgaagagga taaaggagaa atggaatgtc tctaactttg
    28861 tcaacaatac ttccaaaagt cgactgaaga aagaagataa atattaaagg tatgagaact
    28921 cctatcccaa tcatcacatt cgaaaaaata gactgatact ttctgaagac cctagtttga
    28981 gccaagaaat gcactgccac taccatcact agagccacag agacaaataa taaggtcaag
    29041 gacagtagca tcaaaggcaa acccagccat agagaaggag ctagcctaat gtagaggacc
    29101 agaaaataag ctaggattgg tacaattcca gttagagctg gcaaaaggac agacagtcct
    29161 ttagcaatta taatctctga ttctttaaag gcatagggcc tatacgatac caaatcctta
    29221 ctctcataaa agacattgta aaaggccgtt aaagaagttg aaaaggcaat cactagtaaa
    29281 atagcaatca tcgagctaaa ataaataggt atttcctcaa aaggaaaatg aatggctata
    29341 ttactaaaac agatgatcat caagagactg gaaaaaatgt aagaacttaa gactctagcg
    29401 gaaacattta cttttttccc aggattctta gcctgcttct ttcgtagatt agccagatta
    29461 gcttcttgag atgaataaag gatattgata tcaactaatt ttttaatgac cttgagacgc
    29521 atccgcaacc tcctcttttc taccagcaag actaaggtag atactttcca aagactggtc
    29581 tgggtaatct tttctcaagt cctctacact accacaataa atcaaatgcc cctttttcaa
    29641 aatggcaatc cgatcacaga cttgctctgc cacctctagg acatgagttg aaaacaagac
    29701 tgtcttccct ttttgtgcat gttccttcat catctgtttc aaatcaaagg cagcctgggg
    29761 atccaaacca gtcaagggtt catccaagac ccaaatatcg ggatcagaca agagtgctcc
    29821 gatgacaaag actttctgac gcattccgtg agaaagagtt tcaataacct gatagcgatt
    29881 ttcagcaaaa tcaaaaacgt tcaatagcct agctagacta gcctccaagt cagatctact
    29941 cagatcatag gatgaggcga tcaattccca aaattcattg gccgttaagc gtaaaaataa
    30001 gtcaggcgag tctgctacgt agccaatctt tcgtttaata gccaagcgat tttccgataa
    30061 ctcctgaccg tctaccaaaa tacgaccact gctgggtgaa atgatactga ctagggattt
    30121 tatagtggtc gattttccag ccccattatg accaatcaag cccataatct ctccattttc
    30181 aatctgcaaa ttgagattac tcaaggcctc tttatcacca tacaacttac taacattttg
    30241 aaattcaatc atgggacaac tcctatcttt atctgtatta tatatcgcta tactagcact
    30301 ttgatacaaa aaaatcaata ctttatttta agtagttaaa atagtttcct tctatcttac
    30361 gcaaacgttt gcgctaatag atactttatg atagaataaa gaacaagatt gacaagtaag
    30421 aggaaacatt atgcaaaatc aaacactcat gcaatacttt gaatggtatc tgccccacga
    30481 cggtcaacac tggacgcgtc tggctgaaaa tgctccacac ctagctcatc tggggatcag
    30541 tcacgtctgg atgccaccag ccttcaaggc aaccaacgaa aaagatgtcg gctatggggt
    30601 ctatgactta tttgacttag gagaattcaa ccaaaaaggg actgtccgca ccaagtatgg
    30661 tttcaaagaa gactatcttc aagccattca agcccttaaa gcacagggaa ttcaacctat
    30721 ggccgatgta gttctcaacc acaaggctgc tgccgatcac agggaagcct ttcaggttat
    30781 cgaagttgat cctgtagacc gtacagttga acttggagaa cccttcacca tcaatggctg
    30841 gactagtttt accttcgatg gtcgccaaga tacctataat ggcttccact ggcattggta
    30901 ccacttcacc ggtacagact acgatgccaa acgcagtaaa tctgggattt atctgatcca
    30961 aggggacaac aagggctggg ccaacgagga attggtcgat aacgaaaacg gaaactacga
    31021 ctacctcatg tatgccgacc tagactttaa acatcctgaa gtcatccaaa acatctatga
    31081 ctgggctgat tggttcatgg aaacgactgg tgtagctggt ttccgtttgg atgccgttaa
    31141 gcatattgac tctttcttta tgcgcaactt catccgcgat atgaaggaaa aatacggtga
    31201 cgatttctat gtttttggtg aattttggaa cccagacaag gaagccaatc tggactatct
    31261 cgaaaaaacg gaagaacact ttgaccttgt cgatgttcgt ctccaccaga atctctttga
    31321 agccagtcaa gctggcgcaa actatgacct tcgtggcatt ttcacagata gcctggttga
    31381 actcaagcct gacaaggctg tgacttttgt cgacaaccac gatacccaac gaggacaagc
    31441 ccttgagtct accgttgaag aatggttcaa gccagcagcc tatgccctca ttttgttacg
    31501 ccaagacggc cttccatgtg tcttttacgg agactactat gggatttcag ggcagtatgc
    31561 tcaagaagat ttcaaagaaa tccttgaccg cctcctagcc atccgaaaag atttggccta
    31621 tggagaacaa aatgactact ttgaccatgc taactgtatc ggttgggtac gttcaggtgc
    31681 tgaaaatcaa tccccaatcg cagtccttat ctcaaatgac caagaaaaca gcaagtcaat
    31741 gtttgtcggt caagaatgga ctaatcaaac ctttgtagat ttacttggta accaccaagg
    31801 tcaagttaca attgatgagg aaggttatgg acaattccct gtctcagcta gatccgtaag
    31861 tgtctgggca gtcaatacca tctaatagct cataataacc aagctaggtc caagcggatt
    31921 tggctttttt gtattcacaa aaagacctac ccaaatggat agatctttac ttgattacaa
    31981 tttacctgct actgcatcca acaattcttg gatcttaggt tggttgcttc ctcctgccat
    32041 ggccatatct ggtttaccac caccacgtcc atcgatgatt ggtgctaatt ctttgacaag
    32101 gtttcctgca tgaaggtctt ttgtcttgct tgctacaagg acattgactt tgtcaccgat
    32161 agcggcaact aggacaagaa gatcagagta gtctttttgt ttccagttat ctgcaaaagt
    32221 acgaagggca ccggcatcgg atacagacac ttgactagca atgtaacgat gaccgttgac
    32281 ttccttaaca tctttgaaga tatcgcctgc ggctgcagct gcggcttttt ctttcaactc
    32341 agcattttct ttttgaagtt gacgaagttg ttcttgaagt ccttctacct tgtgaggtac
    32401 ttccttgact tgaggtgctt tcaaggttgc tgcgatagct ttaagagcat cctcttgttc
    32461 acgataggct tcaaaggctt ccttaccagt cactgccaag atacggcgag ttcctgaacc
    32521 gattccttct tctttgacaa ttttgaagag accaatctca gaagtgttgt caacatgagt
    32581 accaccacaa agttcaatag agtagtcacc gatagtcacg acacgaactt ccttgccgta
    32641 tttctcacca aagagggcca tagctcccat ttctttagca gtgtcaatat ccgtttcaac
    32701 tgtcttcact tcaagtgctt cccaaatttt ctcgttaact tgctgttcaa tcgcacgaag
    32761 ttcctcagca gttactgctt ggaagtgggt aaagtcaaag cgaaggaatt caacttcgtt
    32821 aagagatcct gcctgtgttg cgtggtttcc aaggatattg tgaagggcag cgtgaagcaa
    32881 atgagtcgca gtgtggtttt tcatgacacg gtgacggcga ttgctatcaa ttgccaaggt
    32941 atattcttgg ttcaaggcaa gcggtgcaag gacttcaact gtatgaaggg cttgaccatt
    33001 tggggctttc tgaacattgg tcacagtagc cacaacctta cctgactcat ccaagatttg
    33061 tccgtagtca gctacctgtc cacccatttc agcataaaat gacgtttccg caaagataag
    33121 agaggcagtt ccttctgaaa cagctcctac ttctgcattg tcagcaacga tagctaccaa
    33181 tttagaagac aattggctag cattgtagtt gaagacactt tctacagtga tgttttgaag
    33241 agtttcattt tgcataccca ttgagccacc cttgacagct gacgcacgcg cgcgttcttg
    33301 ctgttctttc atggctgctt caaaaccttc acggtctaca gtcataccag cttcttcagc
    33361 gatttcttca gtcaattcaa ctgggaaccc ataagtatca tagagtttga agacatctga
    33421 accagcgata acagattgac ctttttcttt caagtctgct acaatgcctt gggcaaagtg
    33481 ttgacctgag tgaagggtac gggcaaatga ttcttcttcg ctcttaacga ttttctcaat
    33541 aaagtcacgt ttctcaagca cttctgggta gtagctttcc atgatttttc caacagttgg
    33601 aaccaatttg taaaggaaag gctcgttgat acccaatttt tgaccatgca tagaagcacg
    33661 acggagaaga cgacgaagaa cataaccacg accttcattt cctggaaggg caccatcacc
    33721 gatagcaaat gaaagagaac gaatgtggtc tgcgataacc ttgaagctca tgttgtcgcc
    33781 atcttggtca taaaccttac cagacaattt ctcgacttca cggataatcg gcatgaagag
    33841 gtccgtttca aagttggtct tagccccttg gataacggcc accaaacgct ccaaaccagc
    33901 gcccgtatca atgttcttat gtggcaattc cttgtattcg ctacgaggaa cagcagggtc
    33961 tgcgttaaat tgtgacaaaa cgatgttcca gatttcaata taacggtcgt tttcaatatc
    34021 ttctgcaagc aggcgaagac cgatattttc tgggtcaaag gcttccccac ggtcaaagaa
    34081 gatttctgta tctggtccag aaggtcccgc accgatttcc cagaagttgt cctcaattgg
    34141 aatcaagtga cttggatcca ctcccacttc aatccagcgg ttgtaagaat ctttatcgtc
    34201 tggatagtag gtcatgtaaa gtttttcagc agggaaatca aaccattcag ggcttgtcaa
    34261 aagctcataa gcccaagtga tagcttcgtc acggaagtaa tccccgatag agaagttccc
    34321 cagcatttca aacatggtat ggtgacgcgc ggtcttccct acgttttcga tgtcgttggt
    34381 acggatagcc ttttgggcat tggtaatacg tggattttca gggataatgg tcccgtcaaa
    34441 gtatttctta agggttgcta ccccagagtt gatccacaaa agagttgggt catttacagg
    34501 aaccaaactt actgatggtt ctactgagtg acctttggtc gcccagaaat caagccacat
    34561 ttggcgtact tgtgcactag atagttgttt catattgtct ccttattcac ttgtttaatg
    34621 tgattggctt tccagcattt ccacatagtc aatcgcgaca cagagggaaa tgactaggtc
    34681 tgcataagcg tcttcaagaa ccgttacggt ataggtagaa gtcagatgga agagttcctt
    34741 cttaatttcc gcaatcaact gatcgcgatc atccagcaat ttgaaattca aatcccagat
    34801 attgccctcg atacgaagac ctagattatc aaactcatac ttatctcgcc agaaggtcaa
    34861 cttcttacga atgacaaaac tcgagccatc ccgaagctga atttcaaaac gaggaagcaa
    34921 ggtcaagatt tctttactaa tctcactgac ttgttcacca gccgcatcat agatggtaaa
    34981 ggttttagga atcttaaaaa atgatccctc cacctgatag gcaatttctc ccctgtcatc
    35041 cttgatagcg aagcgttcgc ctccaagacg aaacttttgt ttgacaagaa atgttttcat
    35101 caacacctcc aaaaatcaaa agacaagctc atatcacgaa gggcgaaaaa ccgcggtacc
    35161 accttcattc aatgaacttg tcattctctt gttcttatgc aattgtatga ttgagtagca
    35221 tgacttccta gcttagatgg ctcgcagcac cgccatttct ctggactaag acaagtgaaa
    35281 atcaattctc aactttctta ttataacgtt tttttaagct tgcgtcaact ggaaatgatc
    35341 tccgttgaat tagaccaatt ccctacatct ctgattactt tttcaggata tattttttct
    35401 tactgccatt tttcttttta tcccaaattt tcatattact aaacacagct actagaatat
    35461 ttccaaatat aaaggtgcct atcacccaat atatggactc agttgttagg tattgtcgat
    35521 ccaagccatc ctttaaatgg aatagtatag cagtttggtt aacaatcata aaggttggcc
    35581 agaaactttt tttgaaaaaa gtagacattt tcattatttg ttgccgcttt ctgtaaggtt
    35641 aatactcaat aaaaatcaaa aagcaaacta ggaagctagc ctcaagctgt acttgagtac
    35701 ggcaaggcaa cgctgacgtg gtttgaagag tataggctta gtatactact aggcaagcaa
    35761 ataaacaaat aaacaactag aatagaaaaa gatagggctc taaaaactga cttctattcc
    35821 ttaaaaacga accagcttga ctgattcgtc ttcttacgtt tatctcctac ttccgataca
    35881 ttttaaactg taggaagagg tcgctatatt tccctgtcca tttatggtca aatttctcat
    35941 aaacttctag gtgtttcatg gtttcaacat cgggatagaa ggccttatct tcctttgttt
    36001 cctctgggag caattccttc gctggtaggt ttggtgttga atagccgaca tactccgcat
    36061 tttggagagc attttcaggt ttcaacataa agttgataaa ggcataggct gagttttggt
    36121 ttttaactgt tttgggaatg accatattgt caaaccaaag attgctggcc tctgtcggta
    36181 ccacataacg tagattttca tttttttcta acatttggct ggcttcacca gagaaggtca
    36241 cgccgattgc aacattattc tgaatcatat agcccttcat ctcgtccgca acgatagcct
    36301 tgatatttgg agtcagtttg tagagcttat ccactgtctc ttccaactgc tgcagatcct
    36361 tggagttgag gctgtagccg agggaattga gtcctagtcc cagcacctca cgcgccccat
    36421 caaagagcat gatagaattc ttatactccg gcttccaaag gtcatcccaa tgctcaggcg
    36481 cttcatctac catggtttcg ttgtagacaa ttcctaaggt tccccagaag taagggatgg
    36541 agaatttatt acctgggtca aaggactggt tgagaaactc tggtccgata ttttcgattc
    36601 cttcaatttt tgaataatca agcggaacca agaggtcttc gtccttcatc ttgttaatca
    36661 tgtattcact tggaatggca atatcgtagg tcgttccacc ctgctttatc ttagtgtaca
    36721 tggcttcgtt ggagtcaaaa gtctcgtact gaacttgaat tcctgtttct tctgtaaact
    36781 gagtcaagag ttcaggatcg atatagtctc cccagttata gataaccaat ttttgactat
    36841 ctcgactatt gattttacta tctaaatgag tcgcaattcc ccacaagaca aggataatcg
    36901 ctgcaattcc tgctaaaaat gaatagattt ttttcatgct tgctcctcct tctcacgaga
    36961 gataaagtaa taacctacaa ctaggataat actaaagaga aagactagag cagacagggc
    37021 attgatttct aaggaaatcc ccttgcgagc acgagagtaa atctcgactg atagggttga
    37081 aaagccattt cctgttacaa agaaggtcac ggcaaagtca tctaacgaat aggtgaaggc
    37141 catgaaataa ccagtaatga tagacggagt caggtaagga agcatgattt ccttgaacat
    37201 ctgaaattga ctagctccca agtcataggc cgcatgaatc atgtcgccat tcatttcctt
    37261 gagtcgaggc aagaccatca agaccacgat aggaatggag aaggccacgt gactagatag
    37321 aacggtcaaa aagccaagtg aaaacttgag ttgggtaaag agaatcaaga agctagcacc
    37381 aatcataacg tcaggcgcaa ccatgaggat attattgagt gatagaaagg cttcttggta
    37441 tttcttacga gactggtaga tgtaaatggc accaaaagtc ccgataatgg tcgctatcaa
    37501 ggctgatagg aaggccaaga aaaatgtctg agccaaaatc agcatgagtc tcccatctcc
    37561 aaacatggtt tcaaagtgag tccagctaaa acctgtaaag ctattcatat catcaccagc
    37621 attaaaggca tagccaatca agtaaaagat aggcaggtag aggaccagaa agaccagtcc
    37681 cagataaagg ttggcaaatt ttttcatcgt tctctccttt ccttagtcac ccacatggtg
    37741 atgaacatgg tcaggatgag aatcacaccg atggttgaac ccataccata gttgtcattg
    37801 gttagaaaat tctgctcaat agccgtcccc aaggtgataa cgcggttccc accaatcaaa
    37861 cgggtcagca tgaagagact caaacttggg ataaagaccg actgaacccc acttcgcaca
    37921 ccgttcatag atagagggaa gatgacatga cggaaggtct cccacttagt tgcaccaagg
    37981 tcataactag cattgatgag attattatcc atatcgtcta agacattgaa aatcggcaaa
    38041 atcataaagg ggagctcgat gtagcttgcg acaaagataa aggaaaaatc ggtaaaaagc
    38101 aactgttgtg aaccaattcc gataaattcc aagaattggt taatagagcc attttgacca
    38161 aaaatcccga taaaagcata ggctttaagg agcaaattga tccaggtagg aaggataatc
    38221 agcatgagcc agagttgacg gtgtttgaga cgggtcaaaa agagggccgt cggataactg
    38281 ataagcagtg ccacaaaggt cacaatgcct gcataaagca ctgagttgaa actcatttta
    38341 agataggtca agttttgtga cgcaaagtaa gatttgtaat tttctaaact gaactggcct
    38401 tcgatgttga aaaaggattg accgaaaatc aagaccaagg gtgccaatac aaagagcgca
    38461 atccaaagca tgtagggtac tacaaagagt ttagagcttg ttttcttcat ctctttcctc
    38521 ctcgattgca ttgatcaaac ctgcttcttg ctcttcgatt tctacgtact cctcaatacg
    38581 agcatcgaac tcttcttcgg tttcattgag acgcatgatg tggatgtctt ctggttcaaa
    38641 gtccagaccg atttcctcac ccacgatagc cttacgggtt gagtggatca tccattcatt
    38701 tccaagttcg tcataggcga taatttcata atgaactcca cggaaaagct gggtatcgac
    38761 cttaacttgg agcttgcctt cttcaggaag ggtaatgcgc aagtcctctg gacgaataac
    38821 gacctcaaca ggttcatttg gcttcatccc accatcaacc gcttcaaagc gtttgccgtt
    38881 aaattcgacc aagtagtcct caatcatggt acctggcaag atgtttgact ccccgataaa
    38941 ggtggcaaca aagtggttga ttggctcatc gtagatgtcc acaggggttc cagactggac
    39001 aatctcgcca tcattcataa cgaaaatcca gtcactcatg gcaagagctt cttcctgatc
    39061 gtgagtgaca aagacaaagg taatgcccaa tcgttgttgt aattcacgca attcgtactg
    39121 catgtctgtt ctcaatttca agtccagcgc tgataaaggc tcgtccaaca agaccacacg
    39181 gggttggttg atgatagcac gggcgatggc cacacgctga cgttgtcctc cagaaagttt
    39241 gcggatggaa cgtttttcat aaccttccaa ctgaaccatc ttgagaactt ccgctacacg
    39301 ctgctcgatt tctttcttat caattttacg caagcgaagt ggaaaggcaa cattttcaaa
    39361 cacattcata tgtgggaaca aggcatagga ttggaagacg gtatgtacgt cgcgcttgtt
    39421 ggttggaata tcattgatac gaacaccgtc tagcatgata tctcctgtcg tcgcatccag
    39481 taaacctgca ataatgttta ggatagttga tttccccgaa ccagatgcac ctagaagggt
    39541 gtagaatttc ccttcttcca actcaaagtt gatgtctttg agaaccttgg tgttgctgtc
    39601 ttcaaaaact ttagagacgt ttttgaattc gataattggc tttttcaatt ggcataaatt
    39661 ccttcttttt catagattaa ccgatcgggg ctctgtcagg tccccactac ctcttgcagg
    39721 gagtaaaacc acctgcatac atcttcgcta ccgataggct ttcacccaag atccggactt
    39781 ctctttcaag cgtaatacct gagtgttcct tgactttttc gataaccgat tggatcaagt
    39841 cctcgtagtc tttggccgtt ccatctgcga cattgatcat aaatcctgca tgcttttctg
    39901 acacttctac gccaccgata cgatagcctt tcaagccagc ttctgaaatt aactgacctg
    39961 caaaatgccc gactggacgc ttaaagaccg agccacaaga tgggtattcc aaaggttgct
    40021 tgagttcacg taggtgcgtc aagcggtcca tttcctgctt gataacctga tgggttcctg
    40081 gagctagggc aaatttaact gacaagacaa ctgcaccaga ctcctgaata gctgaatgac
    40141 ggtaaccaaa agccaagtct ttagcagaca gggtttcgat ttctccatcc ttggtcaaga
    40201 ccttacaaga ctgcaagatg tgagcaatct cgccaccata ggcacccgca ttcataaaga
    40261 cagcaccgcc aacgcttcct ggaataccac aagcaaactc aaagccagtt aaactatgac
    40321 ggagggcaat gcgagttgtt tcaatcaagt tagccccagc ttctgcttca atggtatagc
    40381 catcaacaga aacgttattg agcttgtcac acaagatgac aaatccacga atcccaccat
    40441 cacgaacgat gatattgctt gcattgccaa gaaccatcca agggatattt tcttggttgg
    40501 caaatttcac aacgcgagcc aactcaaaac gatttcgtgg aaagaccaaa taatcagcct
    40561 ctccacctac ttttgtataa ctatagctat gcaagggttc cttaaaacgg atatcaattc
    40621 cttctaagat ttcaagcatt ttttctctta cagacatgtc actcttcctt ttacaaaatt
    40681 cattccatta taccattttt agagacattt gacgaccata aaaatacctt gtttggattt
    40741 tgcataagaa aaagaggttc cccccttttt atgatttttt acaaaagatt tccttggttc
    40801 cataggcgac cagaacgagc tccagtgcta gaatcacttc aaccaagact ggatttgtca
    40861 accagcctac ttggaaaaga gatggtgcca gatcaaagaa ggcatgcaag ccataggctg
    40921 ctaggagata aatccatttc ttctggcgaa cagcttggta aacccaaact gtcaaaagta
    40981 attggaaacc aagcgccaag attcgctcaa aaccaagcaa ataaatctgc cagaccgaaa
    41041 gtgactgaat ggtttttaac atattttcag acagtaattg cataacctgt ggattctgag
    41101 tttgaactgc cgaaagaaca atgtaaagat tgagtaaact agtaaggcct agaaaaatca
    41161 actccaagcc accatgcccc agtccatagg ccaaggcgtc tgccttttcc aaacttctct
    41221 ttttctccaa ccatttgaag aaaacaagac gagccgtttc ctcaaaaaat gctgccatgg
    41281 ctaggccata gataatatag ataagcggat gatcttgcaa gagggcaata ctaccgtctt
    41341 tttgagggtg caaaatcaag atatgcacca gtttttccaa aatctgcgaa gagacaaaga
    41401 aagcgactgc acccaagccc aagacagcta gattaatctg gtatttcttt ttggcaaacc
    41461 aaatgcttcc tatcagaaaa gctagcaaca gcaacatggt aataatgata tgaatggtca
    41521 ttttcttctc ctattctgcc ttttcaatat cttttttcat ctcgtcaaca ttgaacttag
    41581 caaacaagta ttgacggtct tggactggga aacgttggtc caactggtca actgctccca
    41641 cctcgataat gccttccttg atgacaaaat ccatgacatg tccaccgaag gtcaaatcat
    41701 ctgatatgaa gtgcagatgg tagcctgcca cactgacccc atggaaaatc tcaggcgtcc
    41761 agaaaccaac aatagtcccc gcaacattgt cacgactata ttccggttga tgggttgcga
    41821 catcagcaaa cttggtatcg ggtgttgact taggaatcat acgcacatgc atatgtaaaa
    41881 attccccccg aatcttgata gagcggaaaa gattttcccc atcataataa gactcaattc
    41941 gttctttcaa ttccttgtct gtcatctcaa agcgctggcg gaaaatgacc tctgcccgat
    42001 gcggtaccac tgcagcgtaa ggaataaggg catctggtga cacttccaca atttctggct
    42061 ggtctcctga ccctttggcc tgataagcct tgccatccaa gacaatcaat tccccatcaa
    42121 tggaatccaa ggttcccaaa ccaaggtcac catgttctag caattctcct actgtcatag
    42181 taccaccata aaggccagcc atcaaggctc caagagtatt gtattgaaat aatttcactg
    42241 gttcctgcac gttcttatcc attcactttc ttgtctgtta ttctataaat cttactccta
    42301 agtataccac atttgcccct agatgtgaac gagagaaacg ctctagacat tgccaagaag
    42361 gaaaaaaaag ggtacaatgt aacaaaatca agggaggtct ggaatgaaga aacaaagcaa
    42421 gtacaaagag gtcgtttcct atctgaaaaa tggtatcgag tctggacgat ttccgacggg
    42481 tagtcgcctg ccttctatcc gtcaactgag ccttgacttt cactgcagca aggacaccat
    42541 tcaacgagcc ctgctggaat tacggcacga acaatacctc tatgccaagc ctcagagtgg
    42601 ctactatgta ttagaacaag ggcaacatca agacctagaa atcgaggtta ccgacgaaca
    42661 tgccagtgcc tatgacgatt tccgactctg tgtcaatgaa accttgattg gccgagaaaa
    42721 ctacctcttc aactactatg acaatcaaga aggattagaa gacctaagac agtccattca
    42781 caaactcctc tttgagcaag ctctctactg caaggctaac caactagtac tgacttctgg
    42841 aacccaacaa gccttgttta tcctctctca aatatccttt cctagacaag ccaaggaaat
    42901 cttggtggaa cagccaacct accatcggat gaatcgcctc ttgattgcac aggggctgga
    42961 ctatcaaacg attgaacgag gcattgatgg gattgacttg gaggagctgg aaggccactt
    43021 caaaacagga aaaattaagt ttttctacac cattccccga tttcactatc ccctgggaca
    43081 ttcctattct gagcaagaca aacgatctat tcttaactta gctgccaagt atgatgtcta
    43141 tatcgtagag gacgattatc tgggtgattt ggactccaag aagggccaaa ccttccacta
    43201 tcttgataca gaggagcgtg tcatttatat caagtccttc tcgaccagcc tttttcctgc
    43261 ccttcgtatt acagcactca ttcttccaaa tgctatcaaa gaagcatttg tggcctacaa
    43321 aaatatccta gactacgaca gcaacctcat tatgcaaaag gccctgtcac tctatattga
    43381 cagtcaattg tttgaaaaaa atcgtttggc tcgcttgacc aatcatgaat cttaccaaaa
    43441 acaaatcgag gaaaggataa ctaaaacacc ttgtcccctt cctcattatt ccctacacga
    43501 tggtttattg ctagacctga gacagtatcc taaaatcgcc agtctcaaac acagtcaact
    43561 gggcttggac ttctttgaag aggcctattt aagcacctgt ccttatcaat ttgccaaggt
    43621 gtccctagac aatctggaaa atgttttaaa ctatttaaaa gcagaattgg aatgacatcc
    43681 aactctgctt tttcttattc ttcaacttca gttagacttg ctgctttttc aagatagctt
    43741 tggtaagttc cttcatcctt aagtttttgg acgaccttgt ctaccacttc tttcaaatca
    43801 tcactatttt ttctaagagc aacggcattg gcgtcgccgt ccttcatctt caagctgaca
    43861 gttgcgacag ctaagccagc gtttttagca gcataactaa gtgcaacaga ctcatccata
    43921 tgaacagcat ctatttttcc agcctgcaat tcattgactg cttcacccat attagttagg
    43981 gaagttaatt gaacttttgg caattgttcc ttgaccattg attctggaac agtccctttt
    44041 tgggctgcaa tattagcact ttctaggcta gttaaatcct tgtatttttc cacatcagcc
    44101 ttacgaacca agaaactaat cttgttttca tagtatggga ttgaaaaatc aaagacttct
    44161 tttctctcgt cagtagcact aattcctgca actgctaggt cagccttacc agtttgaaga
    44221 ctggtcaaaa cattgtcaaa actcatgctt gagatttcca acttaacccc aagttcatca
    44281 gcgatagcct gagccatgtc gatgtctgca ccgactacct ggttctttcc atcaaccaat
    44341 gattgaaatt caaagggtgc atagtcagga ctggttgcca caactaattt ccctttttgt
    44401 ttgatagcat ccacagcaga ctgagaacca ttagaacctg actgacaagc tactaagaag
    44461 aaacttgcaa gaaaattaca taaaacaaat atccattttt ttgatttcat aaataaacaa
    44521 cctctctgtt aattttgtat aattataacg ttatccaagt tacttgtcaa gtgtttttta
    44581 aatttttatc tcaaaaatat tttttcgttc aaaaaaagga gccatcagtt gatttcaagc
    44641 tcccttttat acagaattaa actattttat agttcgacaa tcttacctgt ttcaaagtag
    44701 acaacccatt cacagatatt tttagcatag tcaccgatac gctccaagta ggaaataact
    44761 tggaaataat cacgacccgt aacaatggct tctggatttt tcttaatctc ttcagtcgca
    44821 aggtcacgga tagtttcaaa atagtggtta atttgctcat ccatggaggc cacccggtat
    44881 gcgtcgtcaa cagaaccatt aagataaaga tcaagtgctg cttccacaac gcttttaact
    44941 tcacgtccca tttttttaat ttcttcctct acagctggaa tgcgctcttc ccccttcata
    45001 cggatggttg cctgggcaat ggctacagcg tgatccccca tacgctccac atctgataca
    45061 gccttaagga cagtcaagac tgtacgcaaa tcttgagaga ctggttgttg gagtgcgatc
    45121 atttcaaatg atttcttttc cagtttcact tcgtattcat ttacttctgc atcatcttcg
    45181 atgacctctt ttgccaggtc acggtcatgc gtgacaaaag cacgtaccgt acgattgatt
    45241 tgtgagagca cttcttgtcc catagcgtag aactggttat gtaatttctc taaatcttct
    45301 tcaaattgag atcgtaacat ctttcatctc cttatccaaa ttttcctgta atatagtctt
    45361 ccgtttcctt gtgttgggga tcaaggaaca tctgcttggt atcattaaat tcaatcaaat
    45421 ctccatctag gaaaaatcct gtcttatcag agatacgtga agcttgctgc atggaacggg
    45481 ttaccagaag catggtgtac ttgtctttta gaccatacaa ggtttcctca attttaccag
    45541 ctgaaatcgg atccaaagcc gaagttggct catccaagag gatgatttta ggactagttg
    45601 ccaagacacg ggccacgcag acacgctgct gttgaccacc tgacaatcca atagctgaat
    45661 catatagacg atccttgacc tcatcccaga tagaggcacc ttgcaaggct ttttctacgg
    45721 cttcatccag aacctgctta tccttaattc cattgatacg aagcccgtag acaacattct
    45781 catagatagt catagggaaa ggattaggtt gttggaaaac cattccgatt tccttacgta
    45841 attcaaccgt atctgtacgc ggactgtaga tgttgtgacc attgtacacc acggatccag
    45901 ttgtggtcac ctctggattg agatctccca tgcggttgag agacttgagg agggttgact
    45961 tccctgatcc agatggacca atcaaggctg taatttcctt aggttggaaa gatagggaaa
    46021 cactattcaa agccttcttt ttattataat aaacggacag gtctgatacc tgtaaaatcg
    46081 catctgtcat acggtttcct ttctaaccaa agtgaccaga tacatagtca ttggtggact
    46141 gtagcttggc attttggaaa atagttgcag tcttgtcata ctcaatcaaa tcacccaagt
    46201 aaaagaagcc tgtatagtca cttgcacgag cagcctgctg catattatgc gttacaatga
    46261 tgatggtaaa gtttttcttg agctcaaaca tggtctcttc tagttgcatg gtcgcaatcg
    46321 gatccaaggc tgaggctggc tcatccatta agaggatatc tggcttaaca gagatggcac
    46381 gagcgataca gagacgttgt tgctgaccac ctgataaggt caaggctgac ttgtggagat
    46441 cgtctttaac ctgatcccag agggcagcct gacgaaggga ggtttctacg atttcatcta
    46501 ggacttgctt atccttaact ccagcacgtt catgcgcaaa ggtaatatta cggtaaattg
    46561 acttagcaaa tggattggga cgttgaaaaa ccattccaat gtgtttacgc atttcataaa
    46621 cgttgatttc tggacggttg acatcaattc cacgatagag aatctgccca gttactttag
    46681 caatatcaat agtatcattc atgcgattga gactgcgtaa gtaggtagat ttccccgatc
    46741 ccgacgggcc aatcaaagct gtaattttat ttctttcaaa ttgcatatca atccccttaa
    46801 tggattcatt tttaccatag taaacatgga catccttagt agaaagggct actttttctt
    46861 caggaaaggt aaggatatgc ttctcatccc agttatatgt tgacatggct tctcctttag
    46921 gcagcggtta atttcttgtg tagatagctt ccgaacttac gagctccaaa gttaaaaatc
    46981 aggataaaga tcaggagcac agcggcagaa cctgctgata caatggttcc atctggaata
    47041 gtgccttcac tattgacttt ccagatatgg acagccaagg tttctgcttg acggaagata
    47101 gagatggggc tagtcacact gaggatattc cagttagacc agtcaagagc tggcgccgat
    47161 tgccctgctg tatagatcag agctgcagct tcgccaaaga tacgaccaga tgccaagacg
    47221 acacccgtta caatacctgg aagcgcttcc ggaataacaa catgaaccac tgtctcccag
    47281 cgagaaatcc caagagccag accagcctca cgttgggtat ggtgaacgtg tttcaaacta
    47341 tcctctacat tacgcgtcat ctgaggcaag ttaaagactg tcaaggccaa ggcacctgaa
    47401 atgattgaaa atccatactc aaactggact acaaagatca agtaaccaaa gagacccacc
    47461 accactgatg gtaaagagga caaaatttca atacaagtcc gcacaaagtt ggtaacagga
    47521 ccttttttag catattcagc caagtaaatc ccagctccca tagaaagagg tacagaaata
    47581 atcaaggtaa tgaccaatag gaaaaaggaa ttgtaaagct gaatgccaat cccaccacct
    47641 gcttgaaaag cagaagacct tccagtcaag aaagaccaag agatatgggg caagccccga
    47701 accaagatat agagaatcaa ggaagccaag attgtcacaa tgatgctagc aatcgtatag
    47761 aggacagctg ttgcaagttt atctaatttc ttagcgcgca taatttttct ttcctctttc
    47821 tttcgtaatc aatttaatca cactgttaaa aactaagctc atcaagagca gtaccaaggc
    47881 cagtgaccag agaacattat tatttacagt tcccatgaca gtgttcccaa ttcccatagt
    47941 taatatagaa gttaaagttg cagctggtgt ggtcaaggaa gttgggataa cagctgagtt
    48001 tccgacaacc atctggatag ctagagcctc accaaaggca cgcgccatcc caaagaccac
    48061 tgcagtgaaa ataccagaac gggccgcctt caagatcaca cgccagatag tctgccagcg
    48121 agtggctccc atagcgaaac tggcttcacg ataataacga ggaaccgcac gcaagctatc
    48181 cgttgtcata aaggttacgg tcggcaaaat catgacaaag aggacggaaa tccctgacaa
    48241 aatcccaaaa ccagtcccac caaagacact gcgaacaaag ggaacgacga cttgcaagcc
    48301 aataaatccg tacactactg aaggaatccc aaccaggagt tcaatagctg gttgcaaaat
    48361 cttcgcccct tttggtgata cttcggtcat aaaaactgct gcaccaatag caaagggtgt
    48421 tgcgataagg gctgagagaa tggtaacgat aaaggaaccc aaaatcatag gaagggcacc
    48481 aaattcttta ctagaaggat tccaagttcc tcccaaaaga aagtcaaaga tattcacacc
    48541 attgacaaag aaggtcgaca agcctttttg cgctacgaaa accaaaatca tggccacaag
    48601 gatgactatc aaagaaagac aggcaaaggt caaacctttt cctaatttct ccagacgaga
    48661 attctttgat ggaagcaaca ttttcttagc taattcttct tgattcatta ttgtctccct
    48721 tccaacactg tcacagttcc ggcagcatct ttttcaacct tcatttcctt aatcggaata
    48781 tacttcaatc ctttgacaat cccttcttgg gtctcatccg agagaacaaa attgagaaat
    48841 tctgcagcca actcattggg ctgccccaat gtatacatat gctcataaga ccacaagggc
    48901 caattattgc tacttatatt ttctggactt aagtcatagc cattcaactt catgcttttg
    48961 accgaatcat ctatataggt aagagataaa taagagatag ctcctggact ttttgatacg
    49021 attgatttta ccgctccatt tgaatcctgc tcctgacttt gcatggcaga ctgaccttcc
    49081 ataatgacag tatcaaaggt agcacgagag ccagagccgg ctgcccgatt gataacagag
    49141 atgggtaagt ccttaccacc aacctctttc caattggtta cctcacctat gaagatttga
    49201 cgaagttgct ctgtcgttag gttatcaaca tcaacctcct tattgacaat cagagccaag
    49261 ccagctaccg cgaccttgtg gtcaacaaga gcagaagcat caattccgtc tttttcctca
    49321 gcaaatacat ctgagtttcc tatatcaact gccccagact gaacctggga caagcctgta
    49381 ccagaacctc ccccttggac attgaccgtt tttccaacat ggatcgtgcc aaattcatct
    49441 gccgctactt caaccaaggg ttgcaaggca gttgagccaa cagccgttat ggattctcca
    49501 cgatcaatcc agctagcaca gcctactaaa caagccgtca gccaaaaagc gataagagac
    49561 agagcaagct tttttctttt tttcactgtt tttctcctcg aaaataatta tgaatactgt
    49621 gaatttttta agtagttctt tatgagttga cgcatgaatt cttaccaaat ttctgcgcaa
    49681 ttgattattt atataatata ggctatatta ctctttccta acctcctttt ttcatatgtg
    49741 gataaaatct cttgtctatc ccttccccca ttgtcaccca ttatagtcat ttcgtgtctc
    49801 tttttcccct ttttaatgca agggaaatta ctctccttag atgataatcc aaaagctaga
    49861 aaggtatctc aaacctctct actctcccag actagtttac aactaaaagg aaaagattct
    49921 attttatgag aaatctagtt tacaagcggt aagaacgcta ataactaaac ttcttgtact
    49981 ctttgaaaat ctcttcaaac cagtgttttg agctatctat ggctagcttc ctagtttgct
    50041 ctttgatttt cattgagtag taaaactaca tgtaatggca atcaagatat caagaatcat
    50101 cctactaaaa aaatccatac tttcactata acatagaata agatatttga ctagcatttt
    50161 catttgaatc tgaggccttt tggaaaataa tttttcaaaa catttccagt aacctttgca
    50221 aagcccaagc cattgccttt aaccaaaact tggtaccaac catttggcag actttctgcc
    50281 agctgaacgg tttctccagc cgcatacttg acaaacgctt cttggccaat ttcaaccgac
    50341 tgttcgacct gactcggttt caaggctaaa ccaagagcga aactgggctc aaagcgtttc
    50401 ttcttaaaag tacccagatg cagtccattg cgagcaatct tgagcttcca taaatctggc
    50461 aaaagttctg gcaagagata aagctggtct ccaaaaatct gcaagatacc cggtagattg
    50521 accttcaaat ggttttgggc aaattcctgc cacaaggcaa cttgttcacg gctgaggtta
    50581 ctcttacttg ccttaaattt aggagctgga ttgttaccct taaactgtag atgggcaaca
    50641 aactgaccct ctcccttaaa ctgatgagga tacatccgag ccgtttctgg caggtcaata
    50701 ccagctacca ttccattgat atgctctact ggcaacaagt caaaatcata ctcttccagc
    50761 aaccaattga caatctcttc gttttcctcg ggtgcccagg tacaggtcga ataaaccaga
    50821 tgaccacctt cagctaacat ggtcactgca tcctccagaa tttctctttg caagctagca
    50881 cattgactcg gataatctaa gctccaatag tccatagcat caggttgctt acgaaacatt
    50941 ccttcaccag agcaaggggc atcaagaacg attaagtcaa aatagccttt aaagaccttg
    51001 accaagcggt cggcagattc attggtcacc acgacatttg tcgctccaaa acgctccatg
    51061 ttttcaacca aaatcttagc ccgtttgctt gaaatttcat tggaaacaag tagcccctcc
    51121 cctgctagat aggctgccag ttgagttgat ttgccccccg gtgcagcagc caagtccaag
    51181 accttcatac caggactggg ttgggctact tgagccacca tttgagcagc aggttcttgc
    51241 gaataaacta aacctgtagc atgctcaggc gatttccctg aaaccttccc atagtggccc
    51301 caaggggttt gagtaatggc atcagaaaag gaaagttgct cttcttttaa gggattgacc
    51361 cgaaaggccg aaaccgcttc ctcctcaaaa gaggcaagaa aatctcttgc ctcatctcct
    51421 agtatctctt tatatttttc aacaaatcct tctggaaatt gcatttaagt tcttttcctt
    51481 tcgtaaatat aggactgaat ttcctcctgc atctcaagag gcaccatcat gaccggctgt
    51541 ctggtttgaa aatcaggagc ttcaccaaaa agggtcacaa cccgatagcc cagactttcc
    51601 cctaaaatac tagctgcggc ataatcccat ggttgcagat aagtgagata ggtcaacaaa
    51661 cgccctgaca aaatcttggc aaaactaatg gccgcacttc catagacacg aacaccaaga
    51721 accgctcggc tcaaatcagc cagcccccat tcattggttt ccagcatacc actattccct
    51781 gcaatgagaa aatctccaag tggtttagtt ttaaaaggag ctagggacct atcatttaga
    51841 caaactggaa attccccacc accgtggtaa caatcccctt tgaccacatc ataaatcaga
    51901 ccaaactgtc cctgaccatt ttcaaaataa gccatcataa cagcaaaatc ttcctgctgg
    51961 gctacaaaat tattggtacc atcaatggga tcaatgaccc aaaccttgcc ctcttgaacc
    52021 gaggctcgca gacaaccttc ttcagcacaa atcttatcct caggataacg ggacaaaatc
    52081 tcaccaacca agagttcctg aacttctttg tccagtctgg tcaccaaatc tgttggagag
    52141 gacttggttt caacacgcaa gtcttcctgc atatggtcaa gaatgtactg acctgctttc
    52201 ttaacaagct ctttagcaaa ttcaaattta ctttccaaga gaaatctttc cttccccttt
    52261 ttctttggca gcctgaactg ctcggtaaag tgaatagcca ctaaccgttt caaattctcg
    52321 tcccaaacgt ttctcttcgc tcttgcttgg cacgaccgct ttgaatccct tataggagtc
    52381 cagtaacttt ttagcctcta ctttgccttc ataggcagct tcaacatcat taaaaaaaga
    52441 aagcactgaa gcaagttctt cagtgctcca cgacaaatct agtgggtaac tatactgttt
    52501 gttcattaac taataccagc tctcattctt gcttctttta gttcttgctt acgataacta
    52561 cgagggagaa aagcacgaat ctcatcttca ttaaaaccga tttgcatacg cttggcatca
    52621 ataataattg gacgacgcaa aagactagga tactgctcaa tcaaatgaag caattccgat
    52681 accgaaatac tctctacatc aatattcaat ttttgaaaaa tttttgaacg agttgaaatg
    52741 atgtcatcag taccattttc ggtcaaggaa aggatgtgtt gcaattcttt tcttgttaaa
    52801 ggactggtca taatattgtg ttccacaaag ggaaccttat gtttttctaa ccaggcctta
    52861 gccttacgac atgatgtaca gctcggtgat agaaatagtg taatcatgct tttctcttct
    52921 tatctatact ttgctacttc tattatacaa aaaaataaag cgcttgacta gggattttta
    52981 gaaaaaaagc ctattttttc aagaaaaata ggctttttgc gaacgattga cacaattgga
    53041 tttggttaat tcactcttaa cgatggtttt aaacgatata tatttttata tatgtaaatt
    53101 aaaaacatct ttcctttcac ttcctacgac ttttcagata cagatagcca aagaagtttt
    53161 catagagggc aaaaaagagg aggaaggcat gaagaaagaa ggtctctggc aaaatcataa
    53221 taacaggatc cttggctgga tcaaaaagcc aggtatcatc tcccacaaag agaatttgat
    53281 ggaaaagagt aaagaattgg tcaaaaccaa tcaaaactcc cccaagtcca atcatcacag
    53341 gtaagactac tagagccagg agactttttc gataaagaga caaaaagtcc tttttcacaa
    53401 tcctattgac aaagacatag aaacttggca gtgtcactag agctactagc tgaaccaaat
    53461 gaaagagatt cttgaccact gcgaaatggt gcagaccagc tgctgacgaa cgaaaatcag
    53521 gcatctgtaa gacctgacta aaaggattgg tcagataatt catcaagata tgaaaattgt
    53581 attgaatggt ttctggtttt agatagactc gattcgttaa gtttagccac tgaatctcca
    53641 taggatagaa aatccaagcc agataaatgg tcagaaggat ggagagggag aggagaaaga
    53701 gcatagagcc ccaaaagatc aatttagttt tcatcaaaat cccactccgc aaggctagaa
    53761 accacatgtg tcggtgcgat tggcaggcca gctacttctt ctgccttagt aaaacctgtc
    53821 gtcaccaaga gcgttggaat gccattgtca atcccagccc gaatatcagt caaataattg
    53881 tccccaacca tgattaactc ttcacgttcc aaacctaagt gctcaaccgc cttgtccata
    53941 atgatggcat ttggttttcc gatataaacc ggcttcactc gtgtcgctac ttcaagcagc
    54001 gtaatcagtg agccagcacc tggcaaaaga ccgcgttccg tcgggatgtt gaggtcagga
    54061 ttggttccga taaaatgggc acccttttga atagcaagag ttgctgtggc aaatttttca
    54121 tagtcgactt gccaatccag accaactacc acgtaggcag gtttttcctt gtcttccaca
    54181 taaccagccg ccttgatggc ttccttgagt cctgcttctc cgacgacata gacggtcttt
    54241 tcaagcccca aatcattcat atagtcgatg gttgccaaag tcgctgtgta gacagtcgat
    54301 aggggcgtat cgatattaaa attctgagcc aacatctcct taacactctc tggagtgcgg
    54361 gttgtattgt tggttacaaa gagataggga atgtcccgct tttgcaattc atgaacaaaa
    54421 gtctctccag cagggattcg gtctttcccc ttataaatgg ttccgtctaa atcaattaaa
    54481 tagcctttat atttcatcta tttctcccta agcctttttt atttcttgcc aagtaatgat
    54541 tgcttgggca ttgataaccc catcacttgt aatttcatgc ttgctttcca gtccagtccg
    54601 ttcaacagcc gatgtaatca ccccacctgg tcgaacttcc ttgacatact tgaggttgat
    54661 tttcttggga atatagtggg tcaaaaaatc cgctcccatg acctcaaaaa tccagtccaa
    54721 gtatttactg ttattgacat gaccattcat atccaagtcg taaaaacgaa catggtaatc
    54781 cttgctgatc ggttcttcca aggactcata cttcggtcca cggataagtt ttttatcaaa
    54841 atcagactgg taaggagcca caatctcagg ttcaacaaca tggacttttc gactgtcgcg
    54901 gtccatgaga acaaaggtcg ccatcatgtg gatgagctcc tgctccgctt cattataaat
    54961 agtaaagcga cggtagcaaa aaagtcgatt gtagctcaag gcttccgttt cgatggtaat
    55021 ttcttccgca aaacgaggca aacgaaccac ctcaatatca tattctacga taatccagac
    55081 cagattatat tcttccaaaa tggccttatc actaactccc agttcaatcg actgcatccc
    55141 tgaaacttgc agtgacagca aaatcacatc tggaagtttg atatgaccgt tcatatcagc
    55201 catatcaaaa ggaattttca ttttcatttg ataagttaag cccatgatcc tactccaaaa
    55261 taaatcgttc tgctacagta tctcccaaaa agagacctct ctttgtcatg cgaacgtggt
    55321 caccctcaat ctgcatgagg ccttgttgaa ccaaatctct gacaatttct ccataaagtc
    55381 cagcaaaaga ctgtccaaat ttttcctcaa atcgcgccat ggaaaccccg gatttcttgc
    55441 ggagtcccaa gaacatttct tcttccattt gctccttttg actcaggtga tcttctgtaa
    55501 tacaagcatt gccttcctca accgcactga gataatgacg aatgggacca tgatttttat
    55561 agcgtactcc attgacataa ccagatgccc ctgcaccaat accatagtat tcagcattgt
    55621 cccagtacat gagattatga cgactttcaa aaccgggttt ggagaaatta gaaatctcat
    55681 aatgctcaaa acccgctcgc tccagctctg caatgatgta ctcaaacatc tccgcttcta
    55741 gttcctcctt aggcagaggc aatttcccac gtcgcatccg gttcataaag accgtatggt
    55801 tttctaaaat caaactatac aaactcatgt ggggaatatc caatccaatg gctttagcca
    55861 cattttcctt tacttgctcc atggtctgac caggcagagc ataaatcaaa tcaatggaga
    55921 tattgtcaaa accagccagt ttcaggcgat cgatattttc ataaatatcc ttctccaaat
    55981 gactgcgccc aatctttttc aacatcttat catcaaaggt ctggacacct agcgaaacac
    56041 gattgacagc cgaatttttc aaaacagcta tcttatccgc atccaaatcg cctggattgg
    56101 cttcaatggt caactcttcc aagacagaca aatccaagtt tttagtcaag ccattcagta
    56161 acacctccag ttgcggagcc gacagggctg tcggtgttcc accaccgata taaagggttg
    56221 acaacttttc aatatcataa gaacgaaact cttccagcag atgctctaaa tagctgtcga
    56281 ctggctgatt tttgatgaag acctttgaaa aatcacaata ataacaaatc tgggtacaaa
    56341 atgggatgtg cacataggct gacgttggtt ttttctgcat agtaattatt ataccacaaa
    56401 gactagattc cagataaaaa tcaccatccc cagatacata gtccgtccgg agatggtgat
    56461 ggtttattct tctgttatat caatcacaat ctcttctgag tcatcaagag cttcggcttt
    56521 ttcttgccat tgctccttga gattatttaa ttgatttttt gatgcttctg tcgcttgaaa
    56581 agcataggat ttagtttgag caagtatact gtccacagtg atttcacctg actcaacctg
    56641 ttcttttgtt ttcagaacaa aatctgtagc ctgctcctta acttctgtca gtttttcaca
    56701 gacttgctcc ttggcatact ccggatcttc tctcaaatca tctagaaaat cttgagcctg
    56761 actgcaaact tgtttgccct tatcacttgt taaaaacaag gcaagagctg cacctgaaac
    56821 ggttcctaaa aggattgagg ataatttacc cataaggatt ctcctttttt attttttgaa
    56881 aaatttactt gcaagacgaa gagctgacag acttgcacca gtcttgagtg tttttgaacc
    56941 agctgatgaa gctttcttgc tcaagacacg cgcatggtca ttgaggtctg aaacagatag
    57001 agataaatct gcaacagcac tgaagagtgg atcaatcgta gccaccttga cattgatatc
    57061 atctgccaag acattgacct tagccaacaa ctcattggtg tgatgcaagg tcacatccac
    57121 atctgaagtc aaggttttaa tcgtcttttc tgtttcatcg atgacacgac caagcttttg
    57181 tacagtaatg atcagataga ccaaaaagac aatcaaagct agggcaacaa gaatatatgc
    57241 aacttctaac atttagtttt cctcctctgt aatatagtaa ggggccttct ttcgattttg
    57301 ataaataacg atcattatac cgagaccgat aaggacaact gacagccatt gggacactcg
    57361 aaagccgaag aacatgagac tatctgttcg cataccttcg ataaccatac gaccgaaacc
    57421 ataccaaatc aagtaaaagg ccgtgatatg acctcgtctg agactcttcc atttccgtct
    57481 aaaaatcaga atcaaggcaa agccaagcag attccataga gactcataaa ggaaagtcgg
    57541 ttgacggtag ctcccctcaa tatacatctg gtcacggata aagccaggta gataatccag
    57601 attatccact gttgcaccat aagcttcttg gttaaagaaa ttaccccaac gccccaaact
    57661 ttgagcaatc ataacgctag gcgccgcaat atctagaaaa tcccaagtat tgatgagttt
    57721 acggtcagca aagatataga gcacaagagc cccagttatc aaaccaccgt aaatggccaa
    57781 accaccattc caaatggcaa aaatctctcc taaattctga ctatagtaat caaatcggaa
    57841 aataacatag tagagacgag ctcctaaaat agccaaggga aaggctacta agataaaatc
    57901 taaaatatcg tctggtatga tcttctttct aggtgcttct ttcatggtca aataaaccgc
    57961 aagaatcaag cctgtcacaa tacataaggc ataccaacga atggctaggg gtcctagttg
    58021 aatagcaatt ggatcaagca ttttgcacct catttcgagc gattagactt gtcagtcgtt
    58081 cgtcgaacaa acgggtcgca tcaaagccca tttccttggc acgataattc atggcagctg
    58141 cctcaatcac aacagagata ttacgacctg ttttaactgg aatacgaata cgaggaatgg
    58201 ctacgccaga aacttcaagt tcctctgcat tatttccaag acgatcaaag gtcttatgcg
    58261 tatcgtaatt ttccaaatag acagcaagct gaacctgtga agaatccttg acagcactcg
    58321 caccgtagag actcataaca tcgataatac caaccccacg aatttcaatc aagtgtttca
    58381 aaatttcagc tggttcaccc cagagagtaa tctcatcctt ggcaaagata tcgacacggt
    58441 catcggctac caaacggtga ccacgtttga caagctcaag acctgtctcg ctcttaccaa
    58501 ttccactatc tccctgaatc aagacgccca tcccataaat atccatcaag acaccgtgca
    58561 cactggtacg ttctgccaaa cgagaatcca gatagctaga taactctcca gataaacgac
    58621 tggtagctgc acggctggtt aaaatagcaa tcttacattc tctagcagcc tttaacatct
    58681 cctctggaac caccaaacca cgggcaacaa tgactgctgg tgtctcaggt agaaacattt
    58741 ttttcaaaac ttcataacgg ctgttggaag gcatgctgat cagataagac cactccttca
    58801 tccccaaaag ttgaatccgc tctggtgtat agtagtcaaa atagcctgtc atttcaagac
    58861 caggtcgcgt aatatccgct gtattgattt ccttttcaag caattctggt tcaccataga
    58921 caatgtctag tctaagcttt tcaatcactt cttttactaa aaccgacatc atttcctcct
    58981 tcaaatgagt tcttctctct attatatcaa attgtaggag gaagtttcag atttttgcag
    59041 gctttggagt atttatttaa aagaaaaagg actagatttc tctagccttt catttcaatt
    59101 agaatttttt acgtttacga gctacttctg atttacgttt acgttttaca gaaggttttt
    59161 catagaattc acgtttgcgt gtttcttgaa gagtaccagc tttagtaacc gcacgtttga
    59221 aacggcgaag tgcgtcgtca agagattcat tcttacgtac tactgtttta gacatttttt
    59281 tcactccctt caagttcaag atctattcta ttttacacgt aaaaggaata attgtcaagc
    59341 aaaaatgcgg tttaattttg attttttctt ccatttatac aagagttgaa agcgttcaat
    59401 agaacattgc ttttttattt ttagagtaag ctaagcgctt cagcatctgc gatgatggtt
    59461 acatcagggt gattttggag gctacttgca ggtaggttct cagtcactgg gccagatact
    59521 gttccggcaa tggcttctgc tttcgactca ccgtaagcaa aaagaataat agacttggca
    59581 tccaaaatgt ttttaatccc cattgaaata gcttgggttg ggacgtcttc aatcttggca
    59641 aagaagcgtg cattggcttc gatagtagac tggtcaagtt ctactagatg cgtttgactg
    59701 tcaaatggag tgccaggctc attaaagccg atatgcccat tgcgaccgat tcccaagatt
    59761 tggaggtcaa ctggatggtc agccaaaatt tggttgtagc gttcaacttc agcttcagca
    59821 ttgtccttaa caccacgagg caagaaactt tctttaaatg gtttttggtt gaacaagttt
    59881 tcttgcatga agtaacgata agactgtgga ttgtccccat caaggcccac atactcatca
    59941 aggttgacac tggttagatt tgaaaaatca aggtcactct caacaatttc cttgtaaaat
    60001 tcaagtgggc tacttcctgt cgcaagtcct agagtttgag ctccattagc caatttttcc
    60061 ttcaaaatct caaaagcaac ttttccacct tgaacttggt tttcaacttt aataactttc
    60121 atcttttcat cctccatttt tgtctatatt tattatatgg tatagaccaa tttttgtcaa
    60181 gtgaaaacga tttaatttct aaaaaaagag cgtccaaact tgaaacgtgt tataatggat
    60241 aggattagaa cttagaaaga atgaacaaat atatgaatac agctgatttt gatttccact
    60301 tgccagagga attgattgcc caaacgcccc ttgaaaaacg tgatgcctcc aaactcctca
    60361 tcgtcaaccg tgagacagga gaaatgcaag ataaacattt ccactctatt attgatatgc
    60421 tggaacctgg tgatgccctt gtcatgaacg acacccgagt tctccctgcc cgcctctatg
    60481 gtcaaaaagt ggagacagga ggtcatgtgg aacttctcct ccttaagaac actagtggag
    60541 acgagtggga agttctggct aaacctgcca aacgcctcaa ggtcggtact cgtatcagct
    60601 ttggtgatgg ccgcctcagc gctgtcgtta cagaagaatt gacccacggg ggacgcattg
    60661 tccgctttga ataccaagga attttcctag aagtcttgga aagtctggga gaaatgcctc
    60721 tgccacctta tatccacgaa aaattagatg accgtgaacg ttatcaaacc gtctacgcca
    60781 aggaaagtgg ctctgctgca gcaccgactg ctggtcttca cttcaccaaa gaactgctgg
    60841 cagaaatcca agctaagggt gttcatctag tctatctgac tctccatgtc ggactcggaa
    60901 cctttagacc tgtttctgtg gataatctgg acgaacacga aatgcactca gagttctatc
    60961 aactttctga ggaagctgct gccacccttc gctctgtcaa aaaaaatggt ggtcgtgtca
    61021 tcgctgtcgg aaccacttct atccgcacct tggaaactat tggttccaag tttgatgggc
    61081 aaatccaagc agattctggt tggaccaata tctttatcaa acctgggtat gagtggaagg
    61141 tcgtggatgc cttctcaacc aacttccacc tgccaaaatc aactctggtc atgttggttt
    61201 ctgcctttgc aggccgtgaa ttagtcttag atgcctacca ccattccatc caagaacact
    61261 accgcttctt cagttttggt gacgccatgt ttatttattg agaaagaatt tctctaaatc
    61321 ttctaatacc aataaatcgc taagatatta tttcaaagaa catctacaat tgaaactcta
    61381 gctagctgta gaagaggcct agtacattga aattaaaatg cttcccccta gcttcgaaaa
    61441 tattgccata gattgcgttg actctccaaa ttgattcatc tatattttat ttcagcttcc
    61501 tatactttct tcgctgtttg taaatcaaaa tgcaagacac atgagtagca ccatatttgt
    61561 tactcttatc tgtcctctca agagactatt atgagttatt tcagaatcat tcactacttt
    61621 gaccctgact ctccttagtc tcaaaatcaa agacttatac tcttcaaaaa tctcttcaaa
    61681 ccgcgtcaac gtcaccttgg attatatatg tgactgactt cgtcagttct atctacaacc
    61741 tcaaagcagt actttgagca acctgcgact agttttctag tttgctcttt gattttcatt
    61801 gagtattaaa caaaaagtga acaaatctga attctaatgt acagaagact aggcttgttc
    61861 acttttttat agtcgctata agatgacctt atctatagct ttttatatat aattatatat
    61921 tcagacatac tattatcaat tttgtcgcag ggaggaatct gttaacgcac ccattcacca
    61981 ttatcattga ctctatagcc atctatactt gtattgaccg ctaactcacc cgatgtattt
    62041 acataatacc atttaccacc aacttggaac cattgattga ctttcataga accgttgctg
    62101 ttgaggtagt accatgaact attaacttgt acccaacctg ttgccatgga accatcagta
    62161 ttataaaaat accacatacc attttcttgt ttccagtctg ttgttggagc aactgcttta
    62221 gctggttcta ctgctacatc tgttccttgg ttagatgtaa cagatacagg atacgaagga
    62281 atagatgatt gctcaggaac aacaactttt tcaggttctc tcgtccctct ccttatacgt
    62341 ctttttacca tctctttagt aatttgacga gaagtagttt cttcaattgt tccatcacgt
    62401 tcatctacag tatagattgt agtaagagta atttaccaat ttctcctact tcttctactt
    62461 cttgactttt atcaagagtt gggccatcga gatattctgt ttcgattgga atttcttgga
    62521 caagaacttg gggcttggtt ctttttttaa caactcttgt ttgagagtct tttttttgac
    62581 ttaaagtact ctcagttact tgtccactct ttccatctac attataagtt atcgttgtaa
    62641 ctgttttccc attctttcct agagtaatct cttgctcctg tcctgcagaa aggtcattgt
    62701 ctgcttcata tttagtagca aatggaacaa gaacttcttc aaccttgctt ttagctggaa
    62761 ctttgataac tgtatccgtg gcttcttttc tatcaacagt aacctgttcg gtaacataac
    62821 cagtctctgg attaacatcg taggtccttg tcgtagttac atagccatcc tctccatcaa
    62881 ttgtaacagg attttcacta cggtcttttg tttcatcttt ttcataacga attcgcgtac
    62941 ttgaaatttt cttggttact accttaggtt tagtcgctac ttttacaata atatccccat
    63001 tgtcagcgtc atcatactct attccctctt ctttatctct agtatcatct ctgacatatt
    63061 gaatcccatc agcagcatga acaaaacttg tattcagatt cctcctaaaa ataaagttag
    63121 cccgattacc gcagaaccaa aaatctttcc gagtttacgt attgcatagc gcttattagt
    63181 attagatttt gccattacat cctacttcta gtatagcatc ttttctatca aacgttaaac
    63241 aatatacttt atatataaaa tagacttaga atgatatatt gattattgaa ctaacacttt
    63301 aactatatcg taatcaatct catatataaa ggattgcaga catcttatct aaatacatgc
    63361 taatatattt agatacaaac attccaactt gataattttc actcatcttt catcattcct
    63421 tatacaacta tgcagtataa atagaatagt tttctcatca gaatgagact attttaatat
    63481 ta
//
LOCUS       DAVKNL010000004        56660 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVKNL010000004 DAVKNL010000000
VERSION     DAVKNL010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 56660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56660
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.004"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(112..1092)
                     /locus_tag="VW411_000210"
     CDS             complement(112..1092)
                     /locus_tag="VW411_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU9793021.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDDFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAVEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            complement(1430..1579)
                     /locus_tag="VW411_000211"
     CDS             complement(1430..1579)
                     /locus_tag="VW411_000211"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793022.1"
                     /translation="MKIKEQTRKLTAGCSKHCFEVVDETDEVSSKHRFEVADRTDEVS
                     NIYIR"
     gene            complement(1607..3622)
                     /gene="recG"
                     /locus_tag="VW411_000212"
     CDS             complement(1607..3622)
                     /gene="recG"
                     /locus_tag="VW411_000212"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEU9793023.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKYLAEYKQALRRIKFEELFYFQMQ
                     LQMLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQVYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            complement(3641..4744)
                     /locus_tag="VW411_000213"
     CDS             complement(3641..4744)
                     /locus_tag="VW411_000213"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU9793024.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVCVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKLYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            complement(4734..5102)
                     /locus_tag="VW411_000214"
     CDS             complement(4734..5102)
                     /locus_tag="VW411_000214"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEU9793025.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            complement(5141..6172)
                     /locus_tag="VW411_000215"
     CDS             complement(5141..6172)
                     /locus_tag="VW411_000215"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU9793026.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            complement(6174..7205)
                     /locus_tag="VW411_000216"
     CDS             complement(6174..7205)
                     /locus_tag="VW411_000216"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU9793027.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            complement(7286..9799)
                     /gene="secA"
                     /locus_tag="VW411_000217"
     CDS             complement(7286..9799)
                     /gene="secA"
                     /locus_tag="VW411_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEU9793028.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQSMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRSLKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            complement(9945..10232)
                     /locus_tag="VW411_000218"
     CDS             complement(9945..10232)
                     /locus_tag="VW411_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793029.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDKPLLGIRIDIFI
                     IQKILLAFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            complement(10260..10892)
                     /locus_tag="VW411_000219"
     CDS             complement(10260..10892)
                     /locus_tag="VW411_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9793030.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGVKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDIEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQM"
     gene            complement(10896..11537)
                     /locus_tag="VW411_000220"
     CDS             complement(10896..11537)
                     /locus_tag="VW411_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793031.1"
                     /translation="MFKNFNNILLNRKIVLLLRIVLMMILINHLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLVLLSLLVMLIVSRLEEKFKKG"
     gene            complement(11530..12321)
                     /locus_tag="VW411_000221"
     CDS             complement(11530..12321)
                     /locus_tag="VW411_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793032.1"
                     /translation="MKIPLLTFARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIITYFFGTFSPLGWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYIVPYMVLEKYEDNV"
     gene            complement(12506..12841)
                     /locus_tag="VW411_000222"
     CDS             complement(12506..12841)
                     /locus_tag="VW411_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793033.1"
                     /translation="MEHLFKMIILLPCFYFFSWIDKDNRESKFFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLAYVFFKKMLDLRRE
                     LGKSKGGRH"
     gene            complement(12981..13316)
                     /locus_tag="VW411_000223"
     CDS             complement(12981..13316)
                     /locus_tag="VW411_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793034.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(13405..14715)
                     /gene="der"
                     /locus_tag="VW411_000224"
     CDS             complement(13405..14715)
                     /gene="der"
                     /locus_tag="VW411_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEU9793035.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(14729..15442)
                     /locus_tag="VW411_000225"
     CDS             complement(14729..15442)
                     /locus_tag="VW411_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEU9793036.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(15439..16335)
                     /gene="dnaI"
                     /locus_tag="VW411_000226"
     CDS             complement(15439..16335)
                     /gene="dnaI"
                     /locus_tag="VW411_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEU9793037.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPKEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTVWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(16336..17505)
                     /locus_tag="VW411_000227"
     CDS             complement(16336..17505)
                     /locus_tag="VW411_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEU9793038.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLGHPVYRRLLEKKIGDVAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKEATIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEEAVLRIRDR
                     GQKAKTQKQNQTAPEKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(17506..17979)
                     /gene="nrdR"
                     /locus_tag="VW411_000228"
     CDS             complement(17506..17979)
                     /gene="nrdR"
                     /locus_tag="VW411_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEU9793039.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            18119..18484
                     /locus_tag="VW411_000229"
     CDS             18119..18484
                     /locus_tag="VW411_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9793040.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            18489..19184
                     /locus_tag="VW411_000230"
     CDS             18489..19184
                     /locus_tag="VW411_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9793041.1"
                     /translation="MSLLVFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            19201..19968
                     /locus_tag="VW411_000231"
     CDS             19201..19968
                     /locus_tag="VW411_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793042.1"
                     /translation="MFWNLVRYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLGISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAVYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVEELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(20380..21579)
                     /locus_tag="VW411_000232"
     CDS             complement(20380..21579)
                     /locus_tag="VW411_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9793043.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINDYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(21576..22469)
                     /locus_tag="VW411_000233"
     CDS             complement(21576..22469)
                     /locus_tag="VW411_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9793044.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFSILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            22586..22666
                     /locus_tag="VW411_000234"
                     /pseudo
     CDS             22586..22666
                     /locus_tag="VW411_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            23176..23370
                     /locus_tag="VW411_000235"
                     /pseudo
     CDS             23176..23370
                     /locus_tag="VW411_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155340.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            23785..24402
                     /locus_tag="VW411_000236"
     CDS             23785..24402
                     /locus_tag="VW411_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEU9793045.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIDWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(24722..25609)
                     /locus_tag="VW411_000237"
     CDS             complement(24722..25609)
                     /locus_tag="VW411_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9793046.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEMVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(25758..27641)
                     /locus_tag="VW411_000238"
     CDS             complement(25758..27641)
                     /locus_tag="VW411_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9793047.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKRAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IVVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFETKV
                     AQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTGDYASVAPVATGSVAKGDAVIE
                     VKI"
     gene            28017..29471
                     /locus_tag="VW411_000239"
     CDS             28017..29471
                     /locus_tag="VW411_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU9793048.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLIHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDKNWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFQGQYYAIVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            29452..30417
                     /locus_tag="VW411_000240"
     CDS             29452..30417
                     /locus_tag="VW411_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9793049.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(30452..31726)
                     /locus_tag="VW411_000241"
     CDS             complement(30452..31726)
                     /locus_tag="VW411_000241"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEU9793050.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VVLSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(31726..32898)
                     /locus_tag="VW411_000242"
     CDS             complement(31726..32898)
                     /locus_tag="VW411_000242"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEU9793051.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            complement(33029..33277)
                     /locus_tag="VW411_000243"
     CDS             complement(33029..33277)
                     /locus_tag="VW411_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793052.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRTYSGNLILQELITVNGAYKATYIGELSSN"
     gene            complement(33345..33968)
                     /locus_tag="VW411_000244"
                     /pseudo
     CDS             complement(33345..33968)
                     /locus_tag="VW411_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(34030..34479)
                     /locus_tag="VW411_000245"
     CDS             complement(34030..34479)
                     /locus_tag="VW411_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU9793053.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(34481..35239)
                     /locus_tag="VW411_000246"
     CDS             complement(34481..35239)
                     /locus_tag="VW411_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU9793054.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAKPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(35354..37333)
                     /gene="pknB"
                     /locus_tag="VW411_000247"
     CDS             complement(35354..37333)
                     /gene="pknB"
                     /locus_tag="VW411_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEU9793055.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSAE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(37330..38070)
                     /locus_tag="VW411_000248"
     CDS             complement(37330..38070)
                     /locus_tag="VW411_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEU9793056.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDAE"
     gene            complement(38085..39398)
                     /gene="rsmB"
                     /locus_tag="VW411_000249"
     CDS             complement(38085..39398)
                     /gene="rsmB"
                     /locus_tag="VW411_000249"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEU9793057.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLLAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(39391..40326)
                     /locus_tag="VW411_000250"
     CDS             complement(39391..40326)
                     /locus_tag="VW411_000250"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU9793058.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(40339..42735)
                     /locus_tag="VW411_000251"
     CDS             complement(40339..42735)
                     /locus_tag="VW411_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEU9793059.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLKSQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRSGRAEKAGQVLIQSYNPQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGSTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(42801..43115)
                     /locus_tag="VW411_000252"
     CDS             complement(42801..43115)
                     /locus_tag="VW411_000252"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEU9793060.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(43140..43766)
                     /gene="gmk"
                     /locus_tag="VW411_000253"
     CDS             complement(43140..43766)
                     /gene="gmk"
                     /locus_tag="VW411_000253"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEU9793061.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(43896..45509)
                     /locus_tag="VW411_000254"
     CDS             complement(43896..45509)
                     /locus_tag="VW411_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEU9793062.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(45700..45954)
                     /locus_tag="VW411_000255"
     CDS             complement(45700..45954)
                     /locus_tag="VW411_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEU9793063.1"
                     /translation="MLLKFTEDAWADYCYWQNQDKKTLKRINKLIKDIQRDPFTGIGK
                     PEPLKYDYQGAWSRRIDAENRLIYMMDGDSVAFLSFKDHY"
     gene            complement(45958..46212)
                     /locus_tag="VW411_000256"
     CDS             complement(45958..46212)
                     /locus_tag="VW411_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEU9793064.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMAQVRAGSTQVHVIEE"
     gene            complement(46450..47547)
                     /locus_tag="VW411_000257"
     CDS             complement(46450..47547)
                     /locus_tag="VW411_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEU9793065.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRTQMALENGADLVVELPFLVSVQAADFFGQGAMDILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGAEMEKFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILMQARESDLPEAIHVLGFTEKGRQHLKSLKGQVSLVSRIGKEPWDA
                     MTQKADQIYQLGKPSIAEQNFGRVPIRIETN"
     gene            complement(47557..48303)
                     /locus_tag="VW411_000258"
     CDS             complement(47557..48303)
                     /locus_tag="VW411_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9793066.1"
                     /translation="MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGK
                     YDFVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHE
                     NAEDFAMLWDTYEDEVPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQ
                     AGFKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            complement(48406..48759)
                     /gene="rsfS"
                     /locus_tag="VW411_000259"
     CDS             complement(48406..48759)
                     /gene="rsfS"
                     /locus_tag="VW411_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEU9793067.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYLVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            complement(48772..49272)
                     /locus_tag="VW411_000260"
     CDS             complement(48772..49272)
                     /locus_tag="VW411_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU9793068.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQFIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            complement(49273..49866)
                     /gene="yqeK"
                     /locus_tag="VW411_000261"
     CDS             complement(49273..49866)
                     /gene="yqeK"
                     /locus_tag="VW411_000261"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEU9793069.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLVLIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEIHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKED"
     gene            complement(49866..50495)
                     /locus_tag="VW411_000262"
     CDS             complement(49866..50495)
                     /locus_tag="VW411_000262"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEU9793070.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKV
                     GTSYPVIWVDVPLMDISSSMVRDFLAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            complement(50547..50858)
                     /gene="yhbY"
                     /locus_tag="VW411_000263"
     CDS             complement(50547..50858)
                     /gene="yhbY"
                     /locus_tag="VW411_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEU9793071.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            complement(51072..52178)
                     /gene="yqeH"
                     /locus_tag="VW411_000264"
     CDS             complement(51072..52178)
                     /gene="yqeH"
                     /locus_tag="VW411_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEU9793072.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGIAKVAVWAPEGVAVVTRKAII"
     gene            complement(52181..52771)
                     /locus_tag="VW411_000265"
     CDS             complement(52181..52771)
                     /locus_tag="VW411_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEU9793073.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            complement(52723..53631)
                     /locus_tag="VW411_000266"
     CDS             complement(52723..53631)
                     /locus_tag="VW411_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU9793074.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESETVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLENHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFV
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            complement(53688..54248)
                     /locus_tag="VW411_000267"
     CDS             complement(53688..54248)
                     /locus_tag="VW411_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEU9793075.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            54387..55596
                     /gene="sstT"
                     /locus_tag="VW411_000268"
                     /pseudo
     CDS             54387..55596
                     /gene="sstT"
                     /locus_tag="VW411_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            55664..56617
                     /locus_tag="VW411_000269"
     CDS             55664..56617
                     /locus_tag="VW411_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU9793076.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
BASE COUNT        16018 a        12958 c        10871 g        16813 t
ORIGIN      
        1 tatttcattt tactatattt ccatgataaa acgcatagta taaagttttc ttaatcccct
       61 gatactatgc gtgtttgtaa tttttaagat tttgtgctta gagtcgactc cttattttag
      121 atatttaaaa ggaatctcac ttccacagag ccagttgtag acttggtcat tgacaaatac
      181 attcatggct tcgtgagcat actcaggcat gatgcgatag gttttatcgc aggtcagacg
      241 attataaatc gcaaactggg taatgggata gcaaacatcg tcgtccaagc ccgtaatcat
      301 cttaacctca ccttggatac gatgggcaag atttttgaca tcgatatagg caagggtcgc
      361 catgatttcc tcctctgttt catggaaggg gtcgtgaaac ttgaaataac ggaaaagttc
      421 gtcgtaagcc tcgctagtat taccaatctc aatcacccgt ctgaagtctg acaagaaggg
      481 ataaatggca actgttttct gaattcgagg attgagcgct gctgcaacta gagctagagc
      541 ccctccttgt gaggcaccat agctagaaag acgcttctca tcaacctgag acagactagc
      601 aacaatttcg accaactggt aaatatccag ataaacatcc ttataaaaga ggtggtcccg
      661 accttccaca gcaccacgga taatatgccc cttcacggta tttcctaaag gagaacgcaa
      721 gccgtcttgt gagtaacctg actggccccg cacatccatg gaaacaacac cgtaaccagc
      781 tacggtgaag cccagcatgt cggcccagtc ccagccacgt cccatataac catgaaaatg
      841 gaagattaat gggaccttct cctcactctt tggaagaaca atgcgtgcat agacctttcc
      901 ttccttgctt ccttcaaatg ttaactcata gcacttgact tgaggaatgt ggaaatctct
      961 ttcctccaag tggtaggatg gaagcgtgga aacatttttc acttccccat cccagaaatc
     1021 atcaaagtct tccggaacct catcccttcc tctataggtc ttaatttctt ctagcaaagc
     1081 tggatttttc atagcatgct ccttttattt tgtaatcgtt accacaactg tagcatatct
     1141 atgtaatcaa tgcaagaaag aagggtaaat agaaagtgaa tttagattct ttacttttag
     1201 gatgatgggc tgaaagtagt tttagaaacg aaaaaagagc atttcgctct ctcttctgtt
     1261 tatactagtt ttttgcttcc ttcttttgga aagtggtttg gtaatttact ttaatcgtta
     1321 ctgtcatatg agagtcctcg tttctttttg atgactctag tataagagcc aaacctaaaa
     1381 actagcttaa tactcttcga aaatctcttc aaaccacgtc agcgtcgcct taccgtatat
     1441 atatgttact gacttcgtca gttctatctg ccacctcaaa acggtgtttt gagctgactt
     1501 cgtcagtttc atccacaacc tcaaaacagt gttttgagca acctgcggtt agcttcctag
     1561 tttgctcttt gattttcatt gagtataagt tttccttaga gaaagcttaa tccagatgtt
     1621 ctttcttttc cagatgaagg gcaatcatgc gccactctgg atcttcttgc caagcttcta
     1681 tagaactaat gtagctagca acctttcttg cttcttctaa aatcggaaaa tcttcgataa
     1741 tatcagccac ttggaactct ggaagtcctg actgtctggt tccaaaaatc tcaccagaac
     1801 cacgcatttt caaatcttcc tccgcaagga caaatccatt ggtcgtttct gtcatgatac
     1861 gcatgcggtc tttcccagaa tccgtcttgg gattagcaac gagaacagcg taggactgct
     1921 tgtccccccg accgacacga cctctaagct ggtgaagttg actgagaccg aagcgatcgg
     1981 catccatgat aatcatgaca gtcgcattgg gaacgttgac cccaacctca ataaccgtcg
     2041 tcgaaaccag aatatccgtc tttctctcct tgaaatcctg catgatctgg tctttttcgt
     2101 cactcttcat cctaccatgt agaagagcca cctctgcctt gcctgcaaaa tgagtcgtca
     2161 actcctctga taaggcaatg gcatttttca aatctagagc ttctgattct tcaatcaaag
     2221 gagagatgac atagacttgg gaaccttttt gaatttcccc ctctaaccaa gtcaagacct
     2281 gaggtagttg ctcatgtttg atccagcgcg tcacaatagg cttccgacct gctggcatct
     2341 ggtcgataat ggaaacatcc atatctccaa aggctgtgat ggcaagcgtc cgtggaatgg
     2401 gagtcgccgt catcatgagg acatctggat tgtcaccttt ttcccgtaaa atacgccttt
     2461 gccctacacc aaaacggtgc tgctcatcga taataatcaa accaagacga gcatactcca
     2521 ccccatcttg tatcagagcg tgagttccta taatcaaatc agcctcaccc ttggcaatgg
     2581 tctccaagac ttctctcttt tctgcagctt tcaaggaacc tgtcaagaga gccagtttca
     2641 aattgggaaa aaggttctgt aaactctcaa agtgttgctc tgcgaggatt tctgttggta
     2701 ccattagggc agcctgataa cctgctgtca ctgccgcaaa catggccaag ccagcgacta
     2761 ccgtttttcc actccccaca tccccttgta ggagacgatt catgtggtgg tcggacttca
     2821 tatcagttaa aatttcctgc aaactctttt cctgagcttg ggtcagggca aaaggaagac
     2881 ttactttaac tgctgtcact ttttcctgag accaattcag aaccagacca cttccctgaa
     2941 ctctattttc agacttgagc atctgcagct gcatttggaa ataaaagagt tcctcaaact
     3001 ttatacggcg aagagcctgc ttgtattctg ccaaatactt tggaaaatgc atagcacgga
     3061 ctgcctgaca acgggacatg agtttgtatt tgtctagtaa agactggggc agattttctt
     3121 ctatcaagag gtccagtccc tgatcaaaag ccgtcttgat gaccttgacc agactggcct
     3181 gactgattcc ctgagccaga cgatagacag gctggaggtc atcttctacc tgagccagaa
     3241 ccttcatccc agtcagacta gccttagcgc ggtcccattt tccaaagaca gcaagggttg
     3301 ctcccaactc tattttatca gccagatagg gctggttaaa gaaattcacc gcaaaaacga
     3361 cctctccctg cttgagacta aaacgcaggc gattgcgctt gaaaccataa tactggacac
     3421 tagcaggagt cactacctga ccagaaagaa ctgccttctc accgtcttcc agctccagca
     3481 cctgcttggt tttgaagtct tcataacgga aaggaaagta gagcaagaga tcttgcaagt
     3541 tttcaattcc tagtttggcg tatttttctg ctgactttgg tcccacacca ggcaagacat
     3601 gcaagggttg atgtagattc atgctccact cctttctttt ctaataatat tctctcggaa
     3661 tacggtcgct gaggaggcaa accacctcat agttaatggt tacgcggtag gtcgctacct
     3721 gagttgcagt gatttcctta tccccattgg agccaatcaa ggttaccttg gttcctagcg
     3781 gataaagctt aggcaatcga atagtgattt ggtccatcga aaccctgccg acaattgggc
     3841 aagcttggcc atctaccaag acagagaaat tttgcatgtc tcttgtccat ccatctgcat
     3901 acccgattgg cacggtcgcg atgacttgct cgctatccgc ttgataagtt gctccatagc
     3961 ccatgcaagc tccagctgga actgtcttga catgaaccag agcagactcc aaggtcaagg
     4021 ccggtatcaa atcataaggc aaatccaaga ccgctccact tggattgagg ccatacatgg
     4081 catctcccat acgaaccgca ttgaaaatag tctctacatg ccaaagagtc gttgcagaat
     4141 tgctagcatg aaccagctct ggaacttcct tcatactagc taaaatagtt ttaaaccgtt
     4201 ctaactgggc attaaaatag tcatctgatt cctcatcagc agtagcaaag tgggtaaaga
     4261 ttccttcaac acaaacaccg tgttgttgga gcaaatcttg agcctgctca acctcacttg
     4321 cctctctaaa accaatccgt cccatccctg aatcaatctt gaggtggact gtcaatccag
     4381 ttaggtccac ttccttatct aagagtgctt gaatccactc cagtccagcc actgtcaagg
     4441 tgaagtcata ttctttagct agagcaacag cttcgatttc agaaactcct aaaatgagga
     4501 ttggcttgct gagtccagct tgtctgagtt caatggcttc atcgatattg gaaacgcaaa
     4561 agccatcaac atcatcttga attgccttgg caacggcaac agctccatga ccataagcat
     4621 tggccttaac cacagccaac ttgagcgttc cttgagggat atgagccccc atttgctgaa
     4681 tattttgtcg aatagctccc agatgaatca gagccttggt tggtctatgt ggactagctt
     4741 tcatgatttt cctccaaaat gacactggct gtcacaaact gatcggtgtg gctgatagac
     4801 agccaaatct ttcctgaaaa tggtgcctga ctaaaataag gcgccccacg ttcattgttc
     4861 aagacttcca aatcctgaaa accgagcttg ctaatgcccg ttcccatagc cttggaaaag
     4921 gcctccttag ccgaccagcg accagctaaa tattctattt gcctgcgtcc tttgagactg
     4981 gtgaagcgct ccatttcctg agcggtcagt acacgcttag caaatccttc atgtcgtgta
     5041 actgcgcttt ctatcgaagc caattcttcg atgtcaattc cgtgtccaac tatcattctc
     5101 atcaaaagga gttgagattc cccaactcca tccttttcac ttattttgtc aaggtaacat
     5161 aaatctcttc taccaaggcc tctgtatttt cccaacctag gcaagggtca gtaatagagc
     5221 aaccaaagat ctctggttgg ttttgacgac catctgctag gtaagattca atcataaatc
     5281 ctcgaaccgt ctttttaatt ttctcattcc aatcacgatt ctgcaaggtc tggcgaacaa
     5341 ttcgaatctg ctccatatat tgcttgcctg agttatcatg gttggtgtca atgaggataa
     5401 aaggattttc aagtcccatg gtttcatagc gttcaatggc ttggagtaga ttttcatagt
     5461 agtaattcgg catataattg ccatactcgt tgactgctcc acggaggata acatgggcca
     5521 aaggattacc tgatgtctca acttcctgcc catgataaag gaaggtttgc ttgttttgag
     5581 cagcatagat ggcgttaaac ataacaccca aatttcctga ggttggattt ttcatcccta
     5641 ctggtgcatc aatcccagaa gccacaaagc ggtgctcttg gtcttccaca gaacgagctc
     5701 caacggcatg gtagctgacc aagtcatcca ccaagatcag atttgacgga taaagcatct
     5761 catctgccgt tgtcaaacca gtctctgtaa tcacgcggta gtgcaactgg cgcacagcct
     5821 gcaagccatt aatcaggctt ggagccttag aagtatctgg ctggtgaact aatcctttat
     5881 agccgtctcc attggtacga ggcttagcag tatacacgcg catgaccatg aaaatcttat
     5941 ccgctacctt cttttgcaag gcagataaac ggcgagcata ttccaagacc gcctcttcat
     6001 tatcagaaga gcaaggacca atcaccaaga gaatacggtc atcttcccct gaaataatat
     6061 ctgccaattc cctgtcacgg ctttccttga gtctcaaggc ttccgcagac aattgggttt
     6121 cagccttgat gacttccata tcgatttctt gacctttttc aataaatgcc atcttattct
     6181 cctaacgttt tgtaaatttc tctgacaaga gcttctgtgt tatcccaacc caggcaaggg
     6241 tctgtgatag acttaccaaa tacttctggc tcattttgtc gaccatcttc cagataagac
     6301 tcaatcataa aaccacgaac gaactgctta attttttcat tccaagcacg gttaatcaag
     6361 gtctggcgga caattcggat ctgttcaata tactgcttac cagaattgtc atgattggta
     6421 tcaatgatga taaaaggatt ttccaagccc attttctcat actgggcaat ggtatcaatt
     6481 aaattgtcat aatagtagtt gggaatattt tttccatact cattaagagc accacgaaga
     6541 atagcgtgtg aaagcgggtt cccagttgtt tctacttctt ttcctaagaa aaggaaactt
     6601 tgtttgtttt gagcagcata aatcccatta aacatgacat tgagatttcc agaggttgga
     6661 tttttaaaac cagtagaaaa tcctgcccca cttgccacaa agcggtgttg ctggtcttca
     6721 actgaacggg caccaactgc catgtaagaa atcaaatcat ctacaagcgg aaggttttca
     6781 ggataaagca tttcatcagc agttgtcatc cctgtttctg tgatgacacg atagtgaaga
     6841 tggcgaacgg ctttgattcc attgataaga ctaggcgctt ctgtcgcgtt aggctggtga
     6901 atcaagccct tatagccatc tccgttggta cggggtttgg cagtataaac acgcataacc
     6961 ataaagatac gatctgccac ttcttcttgt aggactgcca aacgcttagc gtattcaagg
     7021 acagcttctt cgttgtcaga tgagcatggc ccgattacca agagaattcg ctggtcttct
     7081 ccacgtataa tggcttctag ctcttgatct cgctgtgatt tcctctccaa agcctgacct
     7141 tctaattttg acaaggcacg aacttcttct atatttattt tagagctttt tgctgtaaat
     7201 accataactc atctccttat aaagcaaacg gatacccagt aaaaatttac ttttcacaag
     7261 atatccgcct ctaaactatc tcattttatt gtcttttacc gtgacagttt ttaaatttct
     7321 taccagaacc acatgggcaa agttcattgc gtccaatctg gctcaaatcc aaatcttctg
     7381 gcatacttgc ttggtgagca gcgatattgc gagtcgctgt tgtactgata tgacgttctg
     7441 cctgtggtct ttcttgttca tgaatttgtg ctttcatcat caagcgtgtc acatcaaact
     7501 caatcgaacc aatcatatca ttaaacatac ggaaaccttc tgcctgatac tcaacaacag
     7561 ggttgttctg agcatagcca cgaagtccaa ccgcgttacg caattgatca agggcatcga
     7621 tatgatctgt ccacttgtta tccaccactc gtagaatcaa aactttttgg aattctttaa
     7681 ctgcttcttc atcgcgtagt tttgaaacct gactatcgta aaccttcaag gaacgttgga
     7741 aaagctcttc cttgatggcc ttatcagaca agcctgacaa gtcttccatc gtaatagaat
     7801 cttcaggaag caagttgtac ttagcaaagt tcaaaattgc ctctagtttt tcatcttgtt
     7861 tggcacgcgc atgaccatca acgacacgtt caatcgtgcg tttgatcata gactgaattt
     7921 caggtgccaa gtcacgatct gcagtgatga catcgtaacg ttgagcatag ataatctcac
     7981 gttgttcacg catgacatca tcgtattgaa ggacttgttt acgggtatcg tagttatttc
     8041 cttcgacacg tttctgagct gcttcaacct gacgcgtcaa catgcgagac tcaatggcct
     8101 cttcagacat gttcaagcgt tcaaagattc cctttaagcg ttcagaacca aaacgtttca
     8161 tcaaatcatc ttcaagagat aggtagaatt gtgactcacc tggatctcct tgacgacctg
     8221 aacgtccacg aagctggtta tcgatacgac gactttcatg acgttctgta ccaataacac
     8281 aaagtcctcc aagttcacga acaccttcac caagcttgat gtcggtacca cgacccgcca
     8341 tgttggttgc gatggtaacg gcaccacgtt gaccagcatt catgatgatt tgggcttctc
     8401 tatagtggtt tttggcattc aagacttcgt gaggaacacc agctgcaacc aatttcttag
     8461 aaatgtagtc actagtttca accgctactg taccaaccaa gacaggttga cccttttggt
     8521 aacgagcctt aacgtcttcg acaaccgctt taaacttaga ttcgatactt gcataaagaa
     8581 ggtctgagtg gtcaatacgt tgaacaggac ggtttgttgg gattggaata acacgaatgt
     8641 tgtagatttc acggaattct tcttcctcag tcttacctgt acccgtcata ccagacaatt
     8701 tcttgtacat acggaagagg ttttggtacg tgattgaggc agatgtcttg gtttcatcct
     8761 ggattggcac accttctttg gcttcaatag cttggtgcaa tccatcagaa taacgacgac
     8821 cttccatggt acgacctgta aattggtcga caatcaagat ttcttgctct tcgctcacca
     8881 catagtcaat atcgagaagc atgatgtagt tggcacgaag ggcgttatcg ataaagtgag
     8941 tcaaagccac gttttcgatg tcatagaggt tttcaagttt gaagtagctt tcagccctgt
     9001 caatccctga atcagacaaa ccaatagtct tagactgcac atcgatgatg tagtcatctt
     9061 tgttcaaaga ttttacatag tggtctgcca tgtgatacaa ctgactggtt tcaaccgcat
     9121 tggcacctga tacaatcaaa ggtgtacgag cctcgtcaat caagatagag tcaacctcat
     9181 cgaccaaggc atagttaagc ggacgttgta ccatgttttc ggcgcgaacg accatgttgt
     9241 cacgaaggta gtcaaatccg atttctgagt tagttgagta agtaatatca cactcatagg
     9301 cttctttttt ctccattgga gatttggtag ccaagttaat ccctactgac aaaccaagcc
     9361 aagagtacaa ttcacccatc tcagtcgcgt cacgttctga caggtattca ttaaccgtaa
     9421 ctacgtgaac ccctttacct gaaagggcat tgaggtatac cggcatggtc gcagtcaagg
     9481 ttttcccttc ccctgtacgc atctctggca cgtcaccatg gtgaagaaca atccccccca
     9541 tgacctgaac cttataaggg aagagaccta ggacacgttt ggcaccttca cggacaaccg
     9601 caaatgcttc gtaaagcaat gaatccagtg attctccatt ttgataacgt tccttaaatt
     9661 caactgtttt tgcttttagt tggtcgtcag tcaaagcagc catttggtct tcgtatttga
     9721 aaaccttgtc agccatcttt tccagacgac ggatttctcc tttatcattt tcgataattg
     9781 tttttaaaat attagccatg tttttcctta ctttaaattc cgaatatttt agaatgttcc
     9841 tttaatttta gcacaattac tgattatttt caaggaagaa tccccatttt gaaaaggaaa
     9901 aagaggttag ttttcaagct aacctctccg acttttatga ctgtttaatt gccaaaaatc
     9961 ggcaaaatgg caaatgtgat aaataagtta atccaaagag aaagacagca aatcagtcca
    10021 aaaacaaaga gactgacttt cttggacagc aaggccatga gaatggacag aattccaaaa
    10081 gctagcaaga ttttctgtat gatgaagatg tcaatcctta tccccagaag tggtttatcc
    10141 caagggataa agaagaaaga cagccagatc agcagaacta aaccaatata aaccttggaa
    10201 tatcgtgtaa taaatgattt tagatgtgcc ataagccatc tcctaggatt gtatatcctt
    10261 tacatttgta tatgtccatt ttcgacaact aatacatctg tacaaaggtc tgaaatatct
    10321 tccgatatgt gcgacgtcag gataaccaga ccattttctt ttttcaggta agataaaatg
    10381 acctgtttgg ttaacctcac actcttctca tccaaagcat tcataggttc atcgagaaag
    10441 agtatagaag gagaggaaat aaaggcttga atcaaggcca ttttttgctt tgttcctaag
    10501 gataaatgac ggtattcaat gtcttcaaat tcctgtaaat catagtattg aatccaatag
    10561 gcaattcttt tttccgtaac tttagatgaa aaatacctta acagttccaa attttctctc
    10621 agggataaat gagataaaaa ctcgactttt tcaattaaaa ttcctgcatc ctgaatataa
    10681 tgatttttta ccccgtaaac tttaccatct tgaagaactt ttcctttgtc aagcttaata
    10741 taaccagcaa gtatctttaa aagaaccgtc ttgccagatc cattatcacc tttaagtcca
    10801 taaatttttc ctgattgaaa caccaaattc aactgattta aaataggtct tttcccataa
    10861 attttctgtc catttataat ttcaagtctc atcgtttatc ctttcttaaa tttctcttcc
    10921 aagcgactaa caatcaacat aactaataaa cttaagagta ctaaatacac tgttacaata
    10981 tgatccatca tccattcttg tcccacaaat gctaaaaaat agagaaaaaa ggaaaaacca
    11041 gcgcccgcac taatcagtgc tatcagataa gacagtagta agtacaacat cattaactga
    11101 gcaagtaaaa agaaaagtgc gactgttttg aaatgaattc ctagaggaag taaaccaaga
    11161 aaccagacaa acaaatcttg agtaaaaaat ttagaaatga caaccaatcg tttccttgta
    11221 taatcgaaaa aggagcttga ttgatagcga atcaagtgat ggtaatggtc tgccaaatca
    11281 ctttcaagta aaatgacaga gagccatcct accatgaaaa gcgaaaggtt taacaatagt
    11341 ttggggattt ctaaattcgc ttcagaatag tcattatagg aaaaaattga aatcaattct
    11401 ctcttgaaaa agataacagc atcctgcttt tgaaccgctg ttgacaatag atggtttatc
    11461 aaaatcatca tcagaactat acgaagtagt aaaacaatct ttctatttag caaaatattg
    11521 ttaaaattct taaacattat cttcatattt ttcaagcacc atgtaaggga caatataaag
    11581 gatataagga gtaaagagtt tcatcaaact tacatcccca tagctagctt gcatcaaact
    11641 cgtcatagga accatatagt aaggcaaggc actataaagc agcatagaac caagccaaag
    11701 aaacatcaaa taggtgattg ccgaacgagt cacattcccc acataatcaa ctatttgtaa
    11761 aaaatagatt gcattgatga aaataccgat tagtaggaca caagtaaaga acaaatagct
    11821 ttttatctct ccatctagga gtctttgtaa accacttcca tcagaaaata gagaattcca
    11881 tccaagagga gaaaaagtcc caaagaaata ggttataatt gcaattatca gcacagtcac
    11941 taaatatatc aaacaaggga tacttgcaac ttgcaaagtc aactttcttt ttaacccttg
    12001 ataactcact tctcttccaa tactcaatcg taaaacttta tttttcagct caatatattg
    12061 aaaacctagc aaaacaatga tgattggaat aataaatgat tgataaaaac ctagattgaa
    12121 ctggagaata cgaaaatcta atgctgattt taataagtca tttttaattg cttgtccatc
    12181 gaatttagct agatcgggcg catgaatatc aaaaaagaaa taagtcatca aaacatcacg
    12241 ataaaccaaa gcaagaaaaa gcaaagtaag caagacataa acaaatttat gccttgcaaa
    12301 agttaagaga ggaatcttca taagccacct cctacaaata gaaagtaata taatactcaa
    12361 tgaaaatcaa agagcaaact aggaaactag ccgcaagttg ctcaaagcac tgctttgagg
    12421 ttgtagataa gactgacgaa gtcagttaca tatataatcc aaggcgacgt tgacgtggtt
    12481 tgaagagatt ttcgaagagt ataaatcaat gccttccgcc tttagacttc cctagttccc
    12541 gtctcaagtc aagcattttt ttgaaaaaga cataggctaa ccagtcacta gcaaaagcga
    12601 taagtagtaa taatagccaa ataccccata acttgatatc tgtcaaattt ctcaagacga
    12661 tattgaaaaa cagaactgaa aaaactgtcc aagcaaggct aaaaagagca tagagggtga
    12721 tgtaaatcca gtaaaaatag taaaaaattg ggaaaaattt actttctcta ttatccttgt
    12781 caatccagct gaaaaagtaa aaacagggta ataagattat cattttaaac aagtgttcca
    12841 tcacaaacct cctcttttaa attattttct ttagtttcgt tctattaaat gttattacca
    12901 atataaacct tggaatagcg tgtaataaat gattttagag gtaccataag ccacctccta
    12961 caaatagaaa ccgatataaa tcaatgcctt ccacccttag acttccctag ttcctgtctc
    13021 aagcgaaaca tttctttgaa acaggaataa gttaaccaat tcataccaat agctagcaga
    13081 ataaaaagaa accaaatgcc ccataacttg atatctgtca catttctcaa gacggtattg
    13141 aaaaacagaa ctgaaacaac tgtccaagca aggctaaaaa gagaatagaa ggggatgtaa
    13201 aaccagtaaa aataataaaa aattggaaaa aacttactat ttctgttggc cttttcaatc
    13261 cagttatcaa aataaaagta cggtgctaaa agtaagaatt taaacaaatg ttccatcacc
    13321 gacatccccc cttcttttga tagcgttttc tattatttta ttatatcaaa aaaatccgga
    13381 actgtcattc cagattctac ttttttattt gcgttttctt gcgatgagat gaatcggtgt
    13441 tccctcaaaa acaaaggcct tgcggatttg attttccaag aaacgcaggt aagaaaagtg
    13501 catgagttct tcttcattga caaagatgac aaaggttggt ggtttggttg ccacttgggt
    13561 cgcatagaaa atcttgagac gttttccttt gtctgtcggt gttgggttga tggcaatggc
    13621 atccatgatg acatcgttca agacagctga tggaatacgt gtattttgac tttcgctgat
    13681 ttgcttaatc atctcaggaa gtttgtggag acgttgcttg gttaaagctg atacaaagat
    13741 aatcggtgcg taaggcaggt attggaactg ctcacggata tcttcttccc agtttttcat
    13801 agtgtggtta tctttttcaa gcgtatccca cttgttgacc acgataatca tccctttacc
    13861 agcttcatgg gcaaatcctg cgatacgctt gtcgtactca cgaatgcctt cttccgcatt
    13921 gatgaccatc aagaccacat ctgaacggtc aatagcacgc atggcacgca taacagagta
    13981 tttctcagta ttttcataaa ccttaccaga cttacgcata ccagccgtat caatcatggt
    14041 aaactcttga ccatctgtat ctgtaaagtg ggtatcaatg gcatcacgag ttgttccagc
    14101 aacaggacta gcaataacac ggtcttctcc caagatagca ttgatcaagc ttgattttcc
    14161 aacgttagga cgaccaatca agctaaactt aatgacatct ggattttctt cctcatattc
    14221 atttggaaga ttttctacga tcgcatctag cacatcccct gtaccgattc catggacaga
    14281 tgagataggc aatggttcac ccaaaccgag agcatagaaa tcatatatat catttctcat
    14341 ctcagggttg tccaccttgt tgactgcgag gataactggt ttgtgggtct tataaagctt
    14401 acgagctacg tattcgtctg catcagtaat tccttcctta ccagacacga caaaaacgat
    14461 aacatctgct tcttccatgg caatttctgc ctggtgcttg atttgttcca tgaaaggagc
    14521 atcgacatca tcaattcctc ctgtatcaat catgctaaaa gaacgattga gccactcacc
    14581 cgttgcataa atacggtcac gtgtcactcc ttcgacatct tctacaatgg agattcgctc
    14641 accagcgatc cgattaaata gggttgattt cccaacattg ggacgtccta caatggcaat
    14701 agttggtagg gccataattt ctcactttct acaataattt cttctgttca agattttttc
    14761 tagttgagct tggttcagct tgaccaaact gttctgctag gcgctgactc cagcttgtgg
    14821 tcgcacgcgc cccagcatag tcagcctgaa cacggtcata agcttggatt gcctcagttg
    14881 actgttcttg gtattcttcc tcaaagacaa cattctctag tggcagtctc ggtttcatat
    14941 catgatgttg atttggcaca cccagtgcca tcccaaagac agaataggtg tagtcaggta
    15001 ggttaaagag ctctgccact tcttcagact tgtatcgaac caaaccgata atcacaccac
    15061 catagcccaa gctttcagct gccaacaagg cgttttgtcc agcaagagct gcatcgaccg
    15121 aactaatcaa gagaccttcc acaccttggg gttggaaggt gtcggtatga agtcgggctc
    15181 ccttttctgc tcggttcaaa tctccgacaa agagaaggaa aacagcagac tggcgaatgg
    15241 cttcttgagg taccaattca tacaaggcat ctttcttctc ttgacttcgt accacaatca
    15301 cagagtagga ttggaaattc ttccaagatg atgccatctg ggctgctgtc aaaatctcat
    15361 ttaagtctac ttggggaatt tcttgctctt taaacctgcg cactgaagta tgagccttca
    15421 tcaatttaat ggtttctgtc atcgacggtt tactccttct aaacgagtct cctcagccaa
    15481 ataacggatg cgttccatga cccgtctggc ttcccaggtt tcgtcatttc catgtttcac
    15541 tttcgcaaaa tgcttctcca aatcttcaaa gttgaagttg gatgtgaaaa aggtcggtaa
    15601 attttcctgc atccgatatt ggagaatgac ctgcaggatt tcgtcacgca cccaaacggt
    15661 tgattgctcg gcgccaatat catctaaaat caggacctca gacagcttaa tctcatccac
    15721 caaggtctta acattgccat cactgatagc atttttgaca tcaatgacaa agctaggata
    15781 gtggaggaga gttgatgaaa caccacgttt ttctgataaa tcatgagcta aggccgccac
    15841 catgaaactt ttacccacac caaagtctcc atataagtaa agaccttttc gaatagctgg
    15901 atattgctcc acgaaggcta gtagcttttc aaaaactggt aagcgcccca aatcatccaa
    15961 gtcaacttga gccaaactag ctttcttgag actggctggt agattgatta acttgagacg
    16021 gttcttaata gccgcttctt tttcagccgc gattagctca ggagtttctt catatgaaac
    16081 atctgcataa ccatgattct taaccaaaat cggcttgtag cctttggcaa tataatccgt
    16141 atccccacgg agaaacttgt cacgctcggt gatgtactga ttaaacttgg agatactgcg
    16201 atttaattcc tttggagtta aggattcttg ctggataaag gccgcaacat cagggtcctt
    16261 catgattttc tggaccaaat cttgataata aaaacggctg ggttgacgtt tgagtacgtc
    16321 tccgacactt tccatctaat ctcctccttt ttctaatcga gctaatagtt cttgcttctt
    16381 acgttctagt tccagacgag tttcctcgct ggtttcattc ttatattcag gattactcca
    16441 tttaggaaca ttggtttttt ctggggcagt ctgattctgt ttttgtgttt ttgctttctg
    16501 ccctcgatca cgaattcgta aaacggcctc ttctgccgaa tgaatctttt gataggcata
    16561 gtcattggct accttcatgg catatttctc attgatattt gccgaatcca ccttattaaa
    16621 ggtcaataag agaataatat tgatgacttc gtccagtaag cccaagccag ccatctgttg
    16681 caagagttct ctttctgttt gggtaatggt tcccttgcgt gtttgcttga tttctgctaa
    16741 gaactgcagg gcagttttac ttttagcttc tttgataatg gtcgcttcct taagactaaa
    16801 gtcagaggaa actggttttt gagcaatttt ttcacgcatg cgtttggttg aaataacctg
    16861 ggaaacagct gttgacttgg ccaattgata ggtttcaaac caagtccatt tcttctcctc
    16921 ggcaatagca aagaggttta agacatcgga ctgctcatcc gcaaaacgaa gtccatctcg
    16981 agccatcagc tggcgaaaat gttccaagtc aaaatcattg gccactttct tcttgagacc
    17041 aaggtcttct tgactgccta gttctgccaa ttctggaaag acttgattga gtgagacagg
    17101 tatttcttca ccatcagcac tttcaacttt caaatcctcc acagctacat cgccaatctt
    17161 tttctctaag agtctgcgat aaacaggatg ccccaagaag tcttgactag atagaggagc
    17221 atggagggct agctgataaa catcaccctt ttgatagagg gtcaagagat taaaagcaga
    17281 taagattttc aatgatttta tcagtctatc catcccaaag ttgagatggt tgagaatgct
    17341 tgaaaaaaga tattcctttc taccattatc ccaaaaactg attgtataaa gataaaggct
    17401 cagtgcctcc tgaccgataa tcgggaggta gcactgtacc agagatgagg tatcttgcga
    17461 cacccgatta ttctttagat aagaaaaacg gtcaattggc ttcatttatc tttccttttt
    17521 ctttttagag gactgggtga tttgttggag caagctctct aactcactga catccttaaa
    17581 actacgatag acactagcaa aacgtacata ggtaatctcg tccaattcag ccaactcctc
    17641 catgacgagt gaaccaatgt cctcactttg aatttcattt tcatttcgac cacggagttt
    17701 ctgttcgata cgattgacta ccatgttgat ttcatcactt gacacaggac gtttctgggc
    17761 tgagcggata atcccattaa agattttatc tctggagaat tgttcccgtg tgccatcttt
    17821 tttaacaacc actaaggttc tttcttctac tcgttcgtag gttgtaaaac ggtgttggca
    17881 ttcgtcgcac tcacgtcttc tacgaatggt gttcccttct tctgcttggc gactatcgat
    17941 aacacttgac ttggtagccc cacattttgg acaacgcatc ctttccctcc ttatcgtttt
    18001 cttttcatta taccattttt taagcgattc ccaaaacaat tcttcttttt gcttgacaag
    18061 ttttttgttt tgttgtatta tttaattaag acaacaaggt aaaagaaagg agactaagat
    18121 gtcctggaca tttgacaaca aaaaacccat ctatttacag attatggaga aaatcaagct
    18181 tcagattgtt tcccatacac tggaacccaa tcaacaactt ccaaccgtga gggagctagc
    18241 tagcgaggct ggtgtcaatc ccaataccat ccaaagagcc ttatcagacc ttgaacgaga
    18301 aggatttgtc tacagcaagc gaacaactgg acgatttgtg actaaggata aggagctaat
    18361 cgcccagtca cgcaaacaat tatcagaaga agaattggaa cacttcgttt cctccatgac
    18421 ccattttggc tatgaaaaag aagaactacc aggcgtagtc agtgattata ttaaaggagt
    18481 ttaagcctat gtcattacta gtatttgaaa atgtatccaa atcatatgga gcaacaccag
    18541 cccttgaaaa tgtttctctt gacattccag ctggaaaaat tgtcggcctt cttgggccaa
    18601 acggctcagg aaaaacaacc ctgattaaac taattaatgg cctcttacaa ccagatcaag
    18661 gacgtgtcct catcaacgac atggacccaa gcccagcaac caaggccgtt gtagcttatt
    18721 tgcctgatac gacctatctc aatgagcaaa tgaaggtcaa agaagcccta acctacttca
    18781 agaccttcta taaagatttc aatcttgaac gcgcccatca tctacttgca gacctgggca
    18841 ttgatgaaaa tagtcgtctc aagaaactat caaaaggaaa caaagaaaag gttcaactga
    18901 ttttggttat gagccgtgat gctcgtctct atgttttgga cgaacccatt ggtggggtgg
    18961 atccagcagc ccgtgcttat atcctcaata ccattatcaa caactactca ccaacttcta
    19021 ccgttttgat ttctacccac ttgatttctg atatcgagcc aatcttggat gaaattgtct
    19081 tcctaaaaga cggaaaagtc gtccgtcaag gaaatgtaga tgatattcgc tacgagtcag
    19141 gtgaatccat tgaccaactc ttccgtcagg aatttaaggc ctaagcaaag gagattattt
    19201 atgttttgga atttagttcg ctacgaattt aaaaatgtta acaagtggta tttagccctc
    19261 tacgcagccg tgctagtcct ttctgccctc atcggaatac agacacaagg ctttaaaaat
    19321 ctaccttacc aagaaagtca ggctactatg ctactttttc tagctacagt ctttggtggc
    19381 ttgatgctta cacttgggat ttcaaccatt ttcttgatta ttaaacgctt caaaggtagt
    19441 gtctacgacc gacaaggcta tctgactttg accttgccag tttctgaaca ccatatcatc
    19501 acagccaaac taatcggtgc ctttatctgg tcattgatta gcaccgctgt attggctcta
    19561 agtgctgtta ttattctggc tttaacagct ccagaatgga ttcctctttc ttatgtgatt
    19621 acatttgtag aaacacatct ccctcagatc tttcttacag gtatatcctt cctactaaat
    19681 actatttcag gaatcctctg catctacctg gctatttcca ttggacagct tttcaatgaa
    19741 taccgtacag cactcgctgt tgcagtctac attggtatcc aaatcgtcat tggatttatt
    19801 gaacttttct tcaatcttag ttctaatttc tatgtcaatt cactggtagg actcaatgac
    19861 catttctata tgggagcagg tatagccatt gttgaagaac tcatattcat agctatcttt
    19921 tatctcggaa cctactacat cttgagaaat aaggttaatt tgctttaaat aatttttacc
    19981 tagatatgta acatactcat agaacaaaag agaccaggca aaaagtcttt aaaattagaa
    20041 aacgcatagt atcaggtgtt gaatatgtac tgccccccaa aagttagatt ttttctgtct
    20101 aacttttggg ggcagttcat aagaaccttg gtaatatgcg ttttttgtga gctgacttat
    20161 ttcctttcac tatatcgcaa aatgaaataa gaacggaacg atgggatttt ggaattcaaa
    20221 tcaatttata agaatgtttt agaagtaata ttatcctatt ccagattcag ttcactatac
    20281 aattgagttt tcaagcaacc tgtttacata atgtgtacat aattaggttc gtgattccac
    20341 ccttttcacc tttaaaaacc tcgctttcgc aaggctcttc tatttataag ataaggcacg
    20401 tttaaaggtt ttccaaatcc ctaaatcatc cgtttgaaga acgagactag catacatgcg
    20461 tccgataaat cctgttgcta ccaccgcaaa aatcactgta atagcaagtg aaatccatgc
    20521 ttctgctccc cccgcatagt cattaatcgt tcgaaacggc ataaagaagg tcgaaataaa
    20581 gggaatataa gaaccaatct tcaagaggag attgtcacca gctgcaccta gagctgtcac
    20641 tccaaaaaaa ccacccataa tcaaaatcat caaaggcgac aaggctttcc ctgagtcctc
    20701 aggacgagaa accatagatc ctaggaaggc tgccaagact acgtacatga aaagactgat
    20761 caaaataaag agcaaggtat tcagtgagat agcatctccc aagtgatcca aaataccaga
    20821 ctgagccaag aatggcaaat ctttaaagag caaaacggca gccagaccac ctacaacata
    20881 gatcccaata tgcgttaaaa tcactagaaa cagagccatc atccgcgcat agaaatagtg
    20941 acttgccctt atgctagaaa aaacgacttc cataattttg gtgccttttt cactggcaac
    21001 ttcctgagct gttacacccg cataggtaat cagaatcata taaagaaaga atcctaaggc
    21061 acctgctgca attgtttgaa taaacttttt attttccttg gcttcatcaa tcttttctgt
    21121 gaattgaatt gtctgcgcta agcgtttttc ctgctcttga gacaaggaag cagttgaacg
    21181 attaagctga ttttgcagtt cattgagtgt acctgtaacc tcaaatttaa ttccattttc
    21241 aagcgatgtt tcgccatgat aaactgcctt tagaacacta tcttcttgat caatggtcaa
    21301 ataacctttt aatttttctt ctttaattgc ttctttggca cttgcttcgt ctttatagtc
    21361 gaagttaaca ccatttacat tcttcagtcc ttctgctaca gatggcactg ttgtcactac
    21421 tgccacttta ttatttttag ccatagaaga accttggaga tgcccaattc ctacagagat
    21481 tcctaaaaag aggaacggcg aaatcaccat aaagaagaaa ctccatgact cgacatgtcg
    21541 aagataggtt tccttgatta caacccacat atttctcata cttccactcc tgattctagt
    21601 ttaaagattt catcgatagt tggcgcttgt tggtcaaatg ttgcgatata ttgaccttga
    21661 gtcaagattg agaagagttc ccttccagcg ctctcatcct ccaaaatcaa tttccaactg
    21721 ccttgtttgg tcaagctcac ctgtttgaca tgaggaagat tttccaattc ttccttgctt
    21781 cgttcacttg aaacaaagag acgcgttttc ccgtattgat tgcggacatc ctgaactggt
    21841 ccgtgcaaga ccacacggcc atctcggatc atcagaatat cgtcacaaag ttcctcaaca
    21901 ttggtcatga catggtcaga aaagataatg gttgctccgc gctctttttc ctgaaaaatg
    21961 acttgtttga gcaattctgt attaactggg tccaatccac taaaaggctc atccaagata
    22021 atcaggtctg gttcatgaat cagagtaata atgagctgaa tcttctgctg atttcctttt
    22081 gacagactct tgattttatc tgtcagcttt cctttcactt ccaacctctt catccattga
    22141 gggagttttt ctttgacttc tttggcatcc atgcctttta gagtcgccaa gtagcgaact
    22201 tgttcaagaa ctgtcaattt aggcatgaga ctgcgttctt caggcagata accaatccga
    22261 gcataggtct cctgacgaat atcctgacca tccagaccga tttctccctg atattctagg
    22321 aatttcaaaa tactatggaa aatcgttgtt tttccagcac cattttttcc gactagtccc
    22381 aaaatacgac ctggtcgcgc ttgaaagtca ataccaaaca aaacttgctt ggatccaaaa
    22441 cttttctcta gacttcttac ttctagcatc tttcacctcc gaaatttctt gcactcatta
    22501 tactcctttt tgatagcctt tacaatgttt tttgtccatt tttagaagac tattgctgtg
    22561 taaaatatgg cctggagcac ttttatactc aatgaaaatc aaagagcaaa ctaggaagct
    22621 agccgtagac tgctcaaagt acagctttga ggttgcagat aaaactgacg aagtcagctc
    22681 aaaacactgt tttgaggttg tggatagaac tgacgaagtc agtaactata tctacggcaa
    22741 ggcgaagctg acgtggtttg aagagatttt cgaagagtat tagtgataaa tccattatac
    22801 agcagcaaac ttaatttata ccttccgctc ctcaactgtc tatttttaat cctgaattgt
    22861 tatttgagta actccttttt cctcgtaaag ttttcttcct ctaaaacttc tggaaaaagg
    22921 ctaatagttt cagacaacat ttttataaga aacaagttca tctgtcattt caagaaggag
    22981 taatccttta tctactaatg gacggaacag aattcaaccg cttgtccgat atgttttcta
    23041 aggattatat agtaaaatga aataagaaca ggacaaattg atcaggacag tcaaattgat
    23101 ttctaacaat gttttagaag tagatgtata ctattctagt ttcaatctgc tatatctatt
    23161 atgcacaccc ctataggatc taatgaaaat cacaacaggc tcattcatag atggttacct
    23221 aagcctaagg gaactaagaa aacgactacc aaggaagtcg cattcatcga aaagtagatt
    23281 aacaactatc ctaaaaaatg cttgaactac aagtccccca gagaagactt ctggatgact
    23341 aacttgaact tgaaatttag caataattaa ttcactatct aactatattt agtaattatt
    23401 tcagaactga ttaatattaa aattaactaa caattcaaag gattcatact agccataaat
    23461 tacgtccatc agagagagac tcttactact tttagatttt agtctttcta gcttcagaat
    23521 acatctaaac tttagggaaa atgactattc gaaagcgcga atgcctcaaa attatctcag
    23581 ataagctatt cgaaacttag aatgctttta aatttatgga attgcgatta ttcgaaacct
    23641 agaatgcata taacctttag ttgacagacc tattctaagt ctcgaagggc tatttacttt
    23701 ctattcctta tcaaaaaaga ctcattcccc ctttctcctc caaaatatgg tatagtagaa
    23761 atatactatc tatgaggagt ttacatgtca caggataaac aaatgaaagc tgtttctccc
    23821 cttctgcagc gagttatcaa tatctcatcg attgtcggtg gggttgggag tttgattttc
    23881 tgtatttggg cttatcaggc tgggatttta caatccaagg aaaccctctc tgcctttatc
    23941 cagcaggcag gcatctgggg tccacctctc tttatctttt tacagatttt acagactgtc
    24001 gtccctatca ttccaggggc cttgacctcg gtggctgggg tctttatcta cgggcacatc
    24061 atcgggacta tctacaacta tatcggcatc gtgattggct gtgccattat cttttatcta
    24121 gtgcgcctat acggagctgc ctttgtccag tctgtcgtca gcaagcgcac ctacgacaag
    24181 tacatcgact ggctagataa gggcaatcgt tttgaccgct tctttatttt tatgatgatt
    24241 tggcccatta gcccagctga ctttctctgt atgctggctg ccctgaccaa gatgagcttc
    24301 aagcgctaca tgaccatcat cattctgacc aaacccttta ccctcgtggt ttatacctac
    24361 ggtctgacct atattattga ctttttctgg caaatgcttt gacacgtaaa aaatccgttt
    24421 ggtttcccaa gtggattttt aaagcgtaga ttaactatag cttgatacta aatatacttt
    24481 ggtatggaaa tcatgcatat ttttcgatag tgaggcgagg acttacctag cctttccgcc
    24541 gtgatagaaa cacctgaaat ctaatggttt caggtattcg gaaactttga gcctagtgtc
    24601 tcaaagttta ggtatggaat tttgaagaaa gtcgctaccg tccgtaatca cttaaggaaa
    24661 ggctcaaaaa tattgttttc aaccacaaaa tccgtttggt ttcccaagcg gattttgtgc
    24721 tttattttga aacttctttt gcaagaacaa agttcccaag tgtggcagaa ccatttcctg
    24781 cgactgctgg cgtcacgata tagtcacgca catctggtac tggtaggtaa ccattaagaa
    24841 gagatgtaaa tttctcacgg acacggtcca gcatatgttg ttgagccatg acccctccac
    24901 caaagacaat cacgtctggg cggaaagtca ctgtcgcatt aaccgcagct tgagcgatat
    24961 agtaggcttg aacatcccaa acagggttgt tgagttcaat agtttcccca cgtacacctg
    25021 tacgagcttc caaacttgga ccagctgcat aaccttctag acatccctta tggaaaggac
    25081 aaacaccctt aaactctttt tcaatatcca ttgggtgtct agcaacataa taatgaccca
    25141 tttcagggtg acccacacca ccgataaact caccacgttg gatgacgcct gcaccgatac
    25201 ctgtaccgat tgtgtagtaa accaagtttt cgatacgacc accagcattg ttacgggcaa
    25261 ccatttcacc gtaagcagag ctgtttacgt ctgttgtgaa gtacattggc acgtttaggg
    25321 cgcgacgaag ggcaccaagc aagtctacat ttgcccagtt tggttttgga gtcgtcgtga
    25381 taaagccata agtttttgag tttttgtcaa tatcaatcgg cccaaatgaa ccaactgcaa
    25441 gaccagcaag gttatcgaat tttgagaaga actcaatggt tttatcgatt gtttcgattg
    25501 gagttgttgt tggaaattgt gttttttcta caacgttaaa gttttcatca ccgacagcac
    25561 agacaaactt tgtaccgccc gcttccaagc ttccatataa ttttgtcatg ataaacctct
    25621 tgtttttatt ttctttatta tagcatactt cgaaagtcta aatgtctcta ttttttagat
    25681 tttcctctgt aaatcttact atctaataaa aacgaacaaa catgtcattt gttcgttttc
    25741 acattagaga ggattgatta gattttcact tcgatcacag catccccctt agcaactgaa
    25801 cctgttgcga ctggagctac tgaagcgtag tcacctgtat ttgtaacgat aaccattgtt
    25861 gtatcatcaa gtccagctgc agcgattttg tttgagtcaa atgttccaag aacatcgcca
    25921 gctttcacct tattaccttg agcaactttt gtttcaaaac cgtcaccgtt catagataca
    25981 gtatcaatac caacatgaat caaaacttca gcaccatttc ttgttttcaa accaaaagcg
    26041 tgccctgttg gaaaggcaat tgaaacttca gcatcagctg gtgcatagac cacgccttgg
    26101 cttggtttca caacgatacc ttgtcccata gctccacttg agaagactgg gtcattgaca
    26161 tcagcaagag cgacaacatc accgacgata ggagttacaa gtgtttcatt ttgaagagct
    26221 gctggcgcaa cttcttcttt ttcttcagcc acttcagctc gttttgcagc tgcagttgcg
    26281 tctacttcat cttcgtaacc aaacatgtaa gtaagagcaa aaccaagggc aaatgataca
    26341 gctaccataa gaaggtattg tggaagttgt ccgttaccaa cataaagcat tgtaccaggg
    26401 atgatggtga taccattacc agtaccagca agtccaagga tagaagccaa tccaccaccg
    26461 attgcaccag caatcaatga aaggaagaat ggtttacgga agcgcaagtt caccccgaag
    26521 atagcaggct ctgtaatacc taggaaggca gaaagagcag ccgggaaagc aagtgttttc
    26581 agttttggat tttttgtttt aacaccaacc gcaacagtag cagcaccttg agctgtcata
    26641 gcagctgtga tgatagcgtt gaatgggtta gcatggtcag cagcaagtaa ttgcacttca
    26701 agcaagttga agatgtggtg cacacctgac acgacgatca attggtgaac cccaccaatc
    26761 aagaaaccac caagaccaaa tggcatgcta agaatcgctt ttgtagcaat aaggatgtag
    26821 ttttcaacaa cgtggaaaac tggtccaatg acaaagagtc caaggataga catgaccaaa
    26881 agtgtcacga atggtgttac caagaggtca atgacatctg gaacaacctt gcggacagct
    26941 ttttcaaatt tagctccgac aaccccgatg atgaaggctg gaagaacgga accttgcaaa
    27001 ccaacaacag ggatgaaacc aaagaagttc atcgctgtta cttcaccacc ttgagcaact
    27061 gcccaagcgt ttggaagtga gccagagaca agcatcatac caagaacgat accaacggca
    27121 ggatttccac caaatacacg gaaggttgac cacacaacca aacctggcaa gatgatgaag
    27181 gctgtatctg tcaagatttg tgtgtaagtt gcaaagtcac ctggaagtgg catttcaaga
    27241 gcgttgaaaa gaccacgcac acccatgaag agacctgtcg ctacgataac tgggatgatt
    27301 ggaacgaaaa catcaccaaa agtacggata gcacgttgga accagttccc ttgtttagca
    27361 acttctgctt tcatgtcatc cttagatgat gttggtaatc caagtacaac aacttcatcg
    27421 tacattttgt taactgtacc tgtaccaaag ataatttggt attgccctga gttaaagaaa
    27481 gcaccttgaa ctttttccaa gttctcaatc acttctttat tgattttctc ttcatctttg
    27541 accatgacac gtagacgagt cgcacagtgg gcaacactat tgacattttc acgtccgccc
    27601 aaggcatcga tgactttttt tgcaatttcc tgattgttca tttgcaaaaa tctccttata
    27661 taacattttg ttcttgtttg aaagcgattt tattcgccct tacgactatt attttatcat
    27721 gttttaaaaa tatgtcaagc gttttgcaga aaaaaattct atccacttag tgggcaagct
    27781 ttagattgat tgtttttgac tgtttcatca ggaattcctt gaaaaataag tttgaaacct
    27841 tggttaaaaa gtattttatt tcattatctt tcacattttc gtaaaaattt gattgatttc
    27901 ctgtatttat tttatgataa tcctttgaca gtttttcctc ttttttaaca taaaaggctt
    27961 gcatttttta tcgaaaacag ttactattaa aagtgataag atttttggag gaatgaatgg
    28021 aatggacaac tgagcgtcgt tacagacttt atcaagattg gacgcaagaa gaaattcaac
    28081 atataaagga aaatatggca caatctccat ggcatactca ttaccatgtt gagccaaaaa
    28141 caggacttct caacgaccca aatggctttt cttactttga tggcaagtgg atcctctttt
    28201 accagaattt tccttttggt gcagcccacg gtttaaaatc ttgggcacag ctagaaagtg
    28261 atgatttgat tcactttaaa gaaactggaa tcaaagtttt accagatact ccattagata
    28321 gccacggtgc ctactctggt tctgccatgc aatttggcga taacttattc ctattttata
    28381 caggaaatgt tcgcgataaa aactggatcc gtcacccata ccagatcggt gctttgatgg
    28441 acaaggaggg taagattaca aagattgaca agatcttgat tgaccagcca gcagactcta
    28501 ctgaccactt ccgcgatcca caaattttta actttcaggg tcaatattat gccattgtcg
    28561 gcggacaaga cttggagaaa aaaggtttcg ttcgtctcta caaggctgtc aataacgact
    28621 acacaaactg gcaagcagtt ggcgaccttg actttgctaa cgaccgtact gcctacatga
    28681 tggaatgtcc taatttggtc tttgtagagg aacaacctgt ccttctctac tgtccacaag
    28741 gattggataa gaaagttcta gactacgata atatctttcc aaatatgtat aagatcgggg
    28801 cttcctttga ccctaaaaat gccaaaatgg tagatgtgtc tcaacttcaa aacatggatt
    28861 acggtttcga agcctatgca actcaagcct tcaacgctcc tgatgggcgt gctctagcag
    28921 ttagctggct tggtttgcca gatgtttctt acccatctga ccgttttgac caccaaggaa
    28981 ccttctcttt ggtcaaggaa ctcactatca aagacgacaa gctctaccag tatccagtcg
    29041 ctgctattaa ggaccttcgt gcttctgaag aagccttctc aaaccgttcc caaaccaaga
    29101 acacttacga acttgaactc aacttggaag ctaatagcca gagcgagatt gtcttacttg
    29161 ctgataaaga aggtaaggga ctttcaatca actttgacct tgtaaacggt caagtaacag
    29221 tggatcgtag ccaggctgga gaacagtatg cccaagaatt tgggacaact cgttcttgcc
    29281 ctatcgagaa tcaggctact actgctacaa tcttcatcga taactctgtc tttgaaattt
    29341 tcatcaataa aggagaaaaa gtattttctg gtcgtgtctt cccacatgcg gaccaaaatg
    29401 gtatcctgat taaatctgga aacccaactg gaacttacta tgaattagat tatggtcgca
    29461 aaactaactg atgtcgccaa acttgcaggc gtcagtccta ctaccgtttc tcgggttatc
    29521 aataaaaaag ggtatctatc tgagaaaacc atccaaaaag tcaatgaagc catgcgagaa
    29581 ttgggctata aacccaacaa cctggctcgt agtctgcaag gaaaatcagc taagttaatc
    29641 ggcttgattt tccccaatat ttccaatgtt ttctatgcag aattgattga taaattggaa
    29701 caccaactct tcaaaaatgg ttacaagacc atcatctgca acagtgaaca tgattctgag
    29761 aaggaacgcg aatacatcga aatgttggaa gccaatcagg tggacggcat catttctggt
    29821 agtcacaacc taggaatcga agactacaat cgtgtgacag cgccgattat ttcctttgac
    29881 cgaaacctat cgccagacat ccctgtcgtc tcctctgaca actatgctgg tggggttctt
    29941 gctgcccaaa ccttggtcaa gacaggtgcc cagtctatca tcatgattac agggaatgac
    30001 aattctaatt cgccaaccgg actgcgccac gctggttttg catccgtact cccaaaagct
    30061 cctattatca atgtttccag tgacttttct cccgtcagaa aagaaatgga aatcaagaat
    30121 atcttgaccc gggaaaaacc agatgccatt tttgcttcgg atgatttgac agctattctg
    30181 gtcattaaaa tcgctcaaga attgggcatt tctgtcccaa aagagctcaa ggtcatcggc
    30241 tatgatggga cctactttat cgaaaattac taccctcaat tggctactat caagcaacct
    30301 ttggaagaga ttgcttgtct cactattgat cttctcttgc aaaagattga aggcaaggaa
    30361 gtcgccacaa ctggttactt cttaccagtt acgctattac caggaaaaag tatttaaaca
    30421 caagaaaact cagaccgatt cgtctgagtt tttatgatct taaattttcg agatagcgct
    30481 gggctgtctc taggttaaag gttttatctg agatgaggcg ctctactagg ggagcaactt
    30541 cagattcact agccccagct aggagagcta gggatttggc ctgtagtttc atgtggcctt
    30601 gctggatgcc cgtacttacc aaggctttga gggctgcaaa attttgagca agaccgatgg
    30661 acacgataat ctgggctaat tctctggcag aaggatttcc tagtagatca tgactgagaa
    30721 ctacacgtgg gttgaggccg atagagccac ccttagtcgc tacaggcatg ggcagggtca
    30781 tctcaccgac caattcttct ctttcaaggt ccagcgtcca gcagctaaga ccttgatagc
    30841 gtccatctcg actggcaaag gcatgggccc cagcttcgat ggcacgccag tcattaccag
    30901 tggcaatcaa aatcgcatca ataccattaa aaattccttt attatgagta gcagctcggt
    30961 aaggatcagc ctgcgcaaac tgactagcca acgcaatttt ctccgcaatc tctcgtcctt
    31021 gatccttttg gcggctcaag tagcgaaagg cgatgcgaca gcttgcagtc accagagaat
    31081 cggtcgcgta gttggacagg attcccatga gactctgtcc ctgactgagt tcttctaaga
    31141 ctggtttcaa ggcttccagc atggtgttga gcatattggc acccatggct tcctgggtat
    31201 cgacatgaat ataaacaacg agaaagtctg gttcgccttt tatctgctcg acatgcagat
    31261 cacgcgcccc acctccacgt ttaacgatag aaggataggc ttgattggca agctccaaga
    31321 gctccgcttt cttgctggca atcttctctt gcgctagttt aggattagca acttgataaa
    31381 gggctacctg cccaatcatc tgtcgctgat ggacttgtgc agtaaaacca cctgcacgct
    31441 tgatgatttt gctggcatag ctggccgccg caaccacaga gggttcttct gtcacatagg
    31501 gaacggtgta ttcctgaccg ttgacaagta cctccggaac cagtgaataa ggcagagaaa
    31561 aagttcccac tacattctca ctcagctggt ctgccacagt cacgctcatc tgttcatcct
    31621 tctccagact agcttgtctc tcaggactaa ggagcgcctg agcttttaac agctcgaggc
    31681 gctcttggta tgatttttta gaaaatccat tccaacttat cttcattatt tttcaacctt
    31741 gctataacgg cgttggtggt cgagaatttc aaccaaggca aaatcttgat tttcatagcc
    31801 agcaaactgg gcagagttag tttcatccaa gtttacttcc tcaaaaaaga ccttttcata
    31861 gtctgcaacg gatagggcag ttcgttggtt gagcttgttc aaacggtctt tatccaaata
    31921 agcttcatat ccttcaacca attcaccact gaagaactca gccacagctc cacttccgta
    31981 actataaagg gcgattttat ccccagcttt caagctatct gtattttcca agagagacaa
    32041 aagtccaagg aaaagtgaac ctgtgtagat attccccacc ttttgactgt agagaataga
    32101 ctggtcaaaa tgcttttgta agaggtcttt tttctcttga ggcaggctct tatccatgat
    32161 ttttttcaag ccttttagcg ctaatttagg ataaggcaag tggaaacaaa cagccgcaaa
    32221 atcatccaaa gtaagctggt agcgtttttg atattcaagc caagtcgttt tcaaactatc
    32281 caagtattgt tgggtagaat agacaccatt tacataagga gttgtcgagt aatttggtcg
    32341 ccagaaatcc atgatgtcac gggtctgagc tacattgtca ttattaaagg ccatcatgcg
    32401 tggattttgt gtaatcaaca tagctacact tccagcacct tgagttggtt ctcctggagt
    32461 ttcaataccg tatttggcaa tatcactggc aatgaccaag accttggact ccggagaatt
    32521 ttccacatgc aatttggcat aatggagggc agcagtcgct ccgtagcagg cttctttaat
    32581 ctcgaaacta cgagcaaagg gctggatgcc cagcaagcca tgcacaaaga cggccgcagc
    32641 cttactctgg tcaattcctg actcggtcgc cacaatgacc atgtcaactt cttgtctttc
    32701 ttgctcagtt aaaatagagt cactagcact ggccgccaag gtcacgatat cctcagttag
    32761 gggcgcaata ctcaattcct tgagtaagag tcctttactt aatttttcag ggtcaattcc
    32821 cctcgcttct gctaagtctt gtaatttcaa gacatattga ctggtcgcaa aaccaatctt
    32881 atcaataccg attgtcatat ttacctctgt tttatcattc atgtaaaaaa tcgttctata
    32941 ctattttatc acaaatggca gtaaaagaga gaaaaaagac ttgattcacc aaatcaagcc
    33001 tcttattggt catcatttta aagaatgatt agttgctaga gagttcaccg atataagtag
    33061 ctttataagc tccattcaca gttatcagct cctggaggat caaatttcct gagtaagtcc
    33121 ttcccatctc atctacaaat ttttgataaa actgactggt cggaatttct ctgacatcct
    33181 tatcaaatgt cttatcaagt gttttactaa ccttctcagc aatcaattga tgctcttgcc
    33241 atccactttg aaactctgag cccgaactag aaaccatgac tgggataaac aacaaggtca
    33301 gtagatttac agacaataag gaaagtagta gacttcctgc aaaactagaa tcctagttca
    33361 tgattgataa taccagcaat caaattcatt cgtaatccga agcgtttacg atgatttcga
    33421 taggttgttg aaaacatttt aaacgttttt actttggcaa agatgttctc aaccttgctt
    33481 ctctccttag atagcgcatg gttacaggct ttatcttcag ctgttagcgg cttgagtttg
    33541 ctggatttac gtggagtttg tgcttgagga tatatcttca tgagcccttg ataatcactg
    33601 tcagccaaga ttttaccagc ttgtccgata tttctgcaac tcattttgaa caacttcata
    33661 tcatgactat agttcacagc gatatccaaa gaaacaattc tcccttgact tgtgacaatc
    33721 gcttgagcct tcatagcgtg aaatttcttt ttaccagaat cattcgctaa ttgtttttta
    33781 gggcgattga tttttacttc cgtcacatca atcattatcg tgtcctcaaa gctgagagga
    33841 gttcttgaaa tcgtaacacc actttgaaca agagttactt caacccattg gctccgacgg
    33901 attaagttgc tttcgtggat accaaaatca gccgcaattt cttcataagt gcggtattct
    33961 cgcacatata gaaagcgtta tcaatttatt tatctcattt ttcagaaaat tcttttattt
    34021 ctgtaaagtc tacgatactc gatgtgtttt tatataatga tagagtctga gaatcactgt
    34081 tccgctagcc attccaatag agattaccaa agccaacatg acaaccaagg tcgcacttgc
    34141 cagtgcttta ttatagtccc ctgtcacaaa aaaggcagtt gttcggtagg agagataacc
    34201 tggaaccagc ggtgccaaaa tggccaagat aaagaccaca gcaggtgtct tataaagaat
    34261 acttaaaatc tggctgacac aagaaccaat aatggctgca atgaaggtag ctacaatgac
    34321 attggtcggt tccttgagca agagatagat tagccagaca gtcatgccca aaatccctcc
    34381 aggtaagagc atagaccgtt gcacattgag tacgattaaa aaagtgataa tggcaagaaa
    34441 acttgctact gcttgtaata aaaaggttgt tagtgtcata ttagttcatc aataccaagg
    34501 cgacagaagt tcctgcccct aaagcgaggg taatgagcag ggattcaaac atcttactca
    34561 taccagagtt tatgtggttg gtcataatat cacggaccgc attggtcaag gcaatacctg
    34621 gtacaaacgg catgaccgca ccagctataa tcaaatctgc cgttgaagga aaacctgtgt
    34681 agcgagccca aaactgggca attatcccaa agacaaaggc tccagcaaag gctgtcacaa
    34741 agggaattcg gataaatttt tccacataga gggaaaaggc aaaaccaaat aaggtcgcca
    34801 ctcctgcccc aagtgcgtcg tagatatttc cgctaaacat aactgaaaag aaaggagcac
    34861 taaaggtcgc agccagagtt acctgcaact tagtataggg aaggggtttg gcttgcaagg
    34921 ccgtcaattg cttaaaggct gtttctaagt caatctgccc cccaactagc tgacgagaaa
    34981 tctggttcac atcgcagact ttttcgatgt tataagaaga ggaggtcacg cgcttcatgc
    35041 gcgaaatatt ggtattttca atagagaaaa agatagcggc aggcatggca aggacattgc
    35101 aatccacaat cccctgcgaa tgcgcgattc gaatcatggt atcttctaca cgatggattt
    35161 ctgagccact tttaaggaga atagtccccg ctagcataat cacatcaatg acggcattta
    35221 attcttttga ttcttccatg ctttcctcct tttatcaact ccctctattc tatcacaaat
    35281 ccggactcaa aaaaaatctt tgccatgaaa tcatgacaaa gattgattac tcattttgat
    35341 tatccatctg cttttaagga gtagctgaag ttgttttagg tttgtagatt gaaatcttga
    35401 ctctagtctt attgaggtct accttttcac ctgctctagg actttgttca acaaccatgc
    35461 cttctgcact acctgcaggc gctgtcgtca cttctacaac ttctatatta gcttccttaa
    35521 tcccaacaat ttgaatcaaa ttgttcttag taaactccaa gctagaacca atgtaactcg
    35581 gcatggcaac acttgtaact tttttagcta ctgtcaagac aatttgagta ggtttactca
    35641 catcataagt cgttccggca cctggacttt gtttcataat cgttcctggt tcgctttcgc
    35701 tggactcttc ttcctctatc ttaatcaaat tctcaggaac cttcttctgc ttgagttctg
    35761 agattacttc tgtagagttc cgtccaatat agttccctaa ttgaatcgtc gtagcttttt
    35821 tagctactgt caaaacaatt tgagttgcct tgctcaagtc ataggtcgta ccttctggta
    35881 gactttgctt caggaccgtt ccagcctcac tctcattcga ctcttcttcc tcaattttaa
    35941 tcaaattatc tggaactttt ttctctttta attccgcaat gacatcagag gatttccgac
    36001 cgacataatt actaatttgg aaagattgct tgcctgatga gacaaccaaa ttgattttcg
    36061 ttccttcttt tcgaccagtt ccagcgccag gatctgtacg gataatccgc ccttcttcca
    36121 ccttttcact agcctctgtc ttctcctcac caatctcaaa attggctttt ttgagcgttg
    36181 ccttggcctc tgcaactgtc tgacctgcca catctggaat ggcaatggtt gcaggagttc
    36241 tggatagtat ccaaataaga gaagctgcca ccaatacaag gctggccaac aaaatcaggt
    36301 aacgcatctt aaatctatgt tttttcggtg cttgtggttg gtaagtttcc tctgtcacag
    36361 cctggcttgg gtttttgatt gatttgtgtt ctgtttgcgc ttgaacctta ggaatagatg
    36421 tcaaggtact ctgagaaacc ttcggcaagg tcttggtatc tgccttgctc gtttcatcaa
    36481 agattaactt actttcattt ctacgattgt aggacaagct actagacaag tccacataca
    36541 tctctgaaac cgagcggtag cgattggtca actttttagc agttgccttg ataataacat
    36601 tttctaaagc ctgaggtaca gatggatttt ctgcaataac ggacggcagg ggtttctgga
    36661 aatgctggag ggcaatggtc accgcgctat ccccgtcata agggatatgg cctgtcagca
    36721 tctcatagaa aataatcccc atggcataga tatcactctg cacagtcgcc ttcgaaccac
    36781 gcgcctgctc tggtgacaag taatgaactg agcccaacat cgagttagtc tgggtcagac
    36841 ttgtctctgc aaaggctaca gcaatcccaa agtctgtgac cttggcagtc ccatctggtg
    36901 tcaagaggat attttgaggt ttcaagtccc tgtgaacaat tcctcgagta tgggccaagc
    36961 gcatagccaa gagaatttgt cccatgatac ggactgcttc ttcattagaa agaggataat
    37021 gttccttgat atagcgtttg aggtccagtc cagccacata ctccatagct aggtactgtt
    37081 gaccgtcttc ctcgccaata tctgttatcc gaacgatatg aggatggtct agatctgcca
    37141 tagctctcgc ttcacgctga aaacgagcta cagctatcgg gtccgtctgg tagttggtcc
    37201 tcagaacctt cactgccact tcttccccat ctaagattaa gtctttggct aggtagacat
    37261 ccgccatacc tcctcgacca atctgtttga caatccgata gcgtccggca aaaatcttgc
    37321 cgatttggat cattctgcat cctcctcgtt catagaaaca agggcaaccg taatgttgtc
    37381 taaacctcct gcattgttag caaaacgaac aagtgtctcc gttttatctg ctaaaggaat
    37441 atcactggtt acaatatcac gaatctcact gcctgaaatc atgttggtca agccgtcact
    37501 attgagcaag agatagtcac ctgactcaag gataactgtc ccaaaatcag gctgaatttc
    37561 atctttttgc ccaatagact gggtgataat atttttttgc ggatgagctt ctgcctcttc
    37621 tggtgtcaat tgaccagcct tgagcaattc attaaccaag gaatgatcgc tcgtcaactg
    37681 atggtattct tctccacgaa tcaagccgat acgcgaatca ccaatatgag catagatagc
    37741 ctgattatca ataatagcaa ggacttccaa agtagttccc atgcctctgt aagcttcatc
    37801 ctgaccaagc tggtgaatct tttgattttc aatttctagg taatgggcga accattcacg
    37861 cacttcattg actgtatcga tctgggtatc aacccaagct acacccaggt ctgtgaccgc
    37921 catttcacta gcgatattcc ctgcgcgatg acctcccatc ccatcagcta aaataatcat
    37981 ggtacgtcca gctctattga catagtggtt gacatagtct tggttatttg ttcgtttctg
    38041 accaacatct gttaataatg aaatttccat gtgtcagttc cttcctaatc cgatatcttg
    38101 cgaaattgac tgatgaagaa tccatcactt ccatacaatt caggtgtaat gaggatacag
    38161 ccgtctttca tgatatcctt acattcatgt tctagtttta cctgctcgaa ctcgggatga
    38221 ctctctaaaa aggccttaac gacttgaaaa ttctcctctg agacgatagt gcaggtgcta
    38281 taagttatta taccaccttt gcctagtatt tgacaaacac tacctaatat ttctaactga
    38341 atttcctgca aggacgcgaa atctgccgtt tctttattgt atttgatatc tggttttcgg
    38401 cgcaaaagac cgattcctga acaaggagca tccaccaaaa tcttatcaaa ggaatcctgg
    38461 tcaaaaaact catgcacctt tctggcatcc aatttttgag tttgaacccg atcttcaact
    38521 cccagacgtt gggcattttc ttgaattaaa tccaacttgt ggtcgtacaa gtccagagca
    38581 gtaacctgac ctgtcgtaag ataagaggct atatgggctg ttttcccacc tggagccgca
    38641 caggcatcaa gcactcgctc atcaccttgt aaatcaagcg tcggagcaac cagctgactg
    38701 gactcatctt ggatggtaat ggctccatcc gcaaacaaat tatgccctgc aaaatgcccc
    38761 tgctccttaa ccagaccagt ggttgctaaa agggaattat tcgcctccaa caaggcttgg
    38821 atttcctctt ttcgacttag gtctgttaca cgaatactgg ctttgtttcg cactaacagg
    38881 ctttcaaaga tggcttttgc tctctcctct ccgtattctt ccttgagttt ggcaactagc
    38941 caaactggga gagaataggc aatggagtca cgcttgtttt ttcgcttgat gctagcaata
    39001 tctggccagc cttcacgcaa gatacggcga aggacagcgt tgaccaattt ttcactgcct
    39061 tttttacgga gtttggccaa ttccactgct tcattaacca cagcatgatc tggaatcttg
    39121 tccaaatagc ggagttggta ggcactcatg agaagaagga catagagcca gctgtctaac
    39181 tggtctctgt cttcgataaa gtgggatagg taccattcca gagtcagttt acgggctacc
    39241 gttccataga ccagctcggt cactaagccc ttgtctgctg ccaaaagttg acttcccttt
    39301 agatgcttat ttaaggcgat atttgaatat gcttggttca caaaaacatc ctctagcact
    39361 gctagagcta aacttctagc cgtttctact ttagtcacca aatcgttctc ctacagtcaa
    39421 tgtacgtcca actccgttga ggaaggaagc aatgtccatc ttaggcttac cagctggctg
    39481 cacttgtttg agggatagag ccccttcagc cgttgcgaca atcaattctt tcttgccgat
    39541 agagagaatc tcacctggat ttccctgacc ttctactggt agggcttcat aaatcttaaa
    39601 gcggtcgccc ttaaggaaag tatgggcaac aggccagggg ttcattccac gaatttggtt
    39661 aaagagttga cgattggttt tgttccagtc cagtttttct tcctctggct ttatatttgg
    39721 agagaaggta acctgactcg tatcctgcgg ttcaggtttg atatcaccag caatataggc
    39781 aggcagagtg tccaaaagca aatcacgacc aactagcgcc aatttttcaa acaaggtgcc
    39841 aacattgtcc tcatctgtga tcggaatgct gcgacgagaa atcatatctc ctgcatccat
    39901 ttccttaacc atttccatga tggtcacacc agcttcctca tccccttgaa tcaaggcata
    39961 atggataggc gcaccaccac ggtgtctagg aaggagggag gcatgaacgt tgacagcaaa
    40021 gtccatgcta tcaaggagtt tgcttgggag aaactgccca aaagcagcag tcacaattcc
    40081 atctgctcct agcttcataa gatcttccat ctctggactt ccagataatt tttcaggttg
    40141 gtagatagat agtcctgctt ccttggcagc ctgcttgact ggggtttctt ggataacttt
    40201 tttacgacca acagcacggt ctggctgggt cacaacggct agaatttcgt aacggtcatc
    40261 tgtcaaaagt ccttttaaga ctgttgctga aaagtcgggg gtccccataa agattagttt
    40321 tgtcatatct tctccttctt ataaaaattg ctgcggctca tggtcaatgc tgagacggag
    40381 ctcactattt tcccgttctt gagtcaaggc taaaacctgg ttgagggtcg accccagctc
    40441 atcttctaaa cggtatttaa ttaaaatctg gtaatgatag aggttgtggg tacgggcaat
    40501 cggttttggc gttggcccca gaatgggact ggtctctgac aagcctgacc gcaaaatgtt
    40561 catgacttca taggcacgtt tgaaaacctc ttcttctttc ttgtgagaaa gggtaatacc
    40621 aatcgtgaaa tagtaaggtg gatagccgag ttgtcgtctg attcccattt cataggcata
    40681 aaagccttcg taatcttgat ccttggcaaa tcgaatagca tagtgctgcg gattgtagga
    40741 ctgtatcaag acttgacctg ccttttcagc ccgacctgat cgacctgcca cctgagtcaa
    40801 gagctggaag gttctctcag aagaacggaa atcaggcaga ttcaaggccg tatccgcatt
    40861 tagaactccg actagggtaa cattgggaaa atccaaaccc tttgcaatca tctgagtacc
    40921 aagtaaaata tccgcttccc ctcgcccaaa ctggtcaagc aaggcttggt gactgccttt
    40981 ctttcgagtc gtatccacat ccatcctcag aatgcgagct tggggaaaga gttctgctag
    41041 ctcatcataa gccttctgag ttcccgtccc atagtaacga atactgcggc tcttacagtt
    41101 aggacagacc tgaggaatat ccttcgagaa accacaataa tggcagttca tagtcttggt
    41161 atccatatgc aaggtcagag aaatatcgca gttgggacaa gtatccaccg tcccacactc
    41221 ccgacacatg acaaagctag aataaccacg gcgattgagc atgagaacca cctgctcttt
    41281 tttaaccaga cggtcttgga tagcctctag caaaggaggc gtaaagtttg acgtctcatt
    41341 ttgtccgata tagtctcgaa agtcaatcac ttgaacctca gggattgtag ccaaaggatt
    41401 ggcacgttgg gttagacgta agtgttgata gacgcctttg ccagcacgtg cccggctctc
    41461 taagctcggc gttgcagatc caagtaccag agttgcttga ttatactgag cccgtaaaat
    41521 agctacctct ctggcatggt aacggggatt gctgtcctgc ttataagccg cttcatgctc
    41581 ttcatcaata atcatgacac ccagattttt cagaggagca aagatagcag atctggcacc
    41641 aacaacaact tgggcatcgc cacgctccac cttgcgccat tcatcatact tttcaccatt
    41701 ggataatcct gagtgaagaa tggctacctt gtccccaaaa cgtgctataa aacgctcggt
    41761 catctgagga gtcaaggaaa tctcaggtac cagcaaaata gctgtcttgc ccttatccag
    41821 ggcaccttgg ataatctgca agtaaacctc ggtcttccca cttcctgtaa tcccttgaag
    41881 tagaaaggga ggttgagaac tgccaataga actcacaacc gcatcacgcg cctgtctttg
    41941 ttctggattt aactccaaag gtctacttgc ttcaattcct tcaaaataag cagccgagcg
    42001 ttgaacttcc ttttggacta tggtaacagc accttgatcc acaaagaagt tgacttgctc
    42061 tcgcgagtag gactctaaca agctagccaa ggaagcgctc tctggatgag acagcagata
    42121 atctctcagt tccaactttt tcttggcacg tgtagaaatc tcaacacctt ctaattgagc
    42181 atggtcaacc tcataccaag actgggtctt gaccttcttt tgatcgactg cctgatattc
    42241 cagaccaagc aggccttttc tagtcaaacg catcatttca gcttgcttgg caaggtctag
    42301 tgaagaaaag gctagcgaat cttctgaacc aaacaggcgc actcgttctt cctgactcaa
    42361 gccttccaga ggatagagaa tcttgtcata gctagaattc agaaaccctg gaagcatggc
    42421 cttgaggata gagattttgt aggagaagac agatttgcgt aactcctcag ccagccagag
    42481 ttgttctggc gtgagaacag gagaaaaatc cagcacctct gcaatatctt ttaaatcttg
    42541 ctccatctct tctccatctg attgggactt caaaccaaga acaatccctt gaatcaggcg
    42601 attaccctta ccaaaaggca catgaacccg catcccaact tccagcattc cctcaaattc
    42661 ctccggaatc ctgtaactat agggctggtc cgtctgcatc aagggcacat ctacgataat
    42721 cttagctagg gccatcttct cacctcctcc ttgtcagtac attcttgcaa tagaaaaaat
    42781 aagattgagt ccccccaacc ttaaattttt tcaccatctt ctttttcttt agcaatttgc
    42841 tctttgattt tcttttcttc ttcttctttg cggcgttttt cttcttcgat acggcgacgc
    42901 actgcttcac gttttccttc tggatctggg tgaattgtaa cgtttcctga ttcgatttct
    42961 tctaaagcgc gaagagttga tttttcagac ttgaaacctt gagttgctgg ggcacctgct
    43021 tccaattcgt gggcacgttt tgcttccaag attacgagtg aatattttga aggaaccttg
    43081 tcgagcaagg tatcaataga gggttttaac atcatttgct tgtacctatt ttctaaattt
    43141 tatcgggtag ttggagattt tggtaacatc tcctgatagt gaccaatgac acgatccaca
    43201 cagaagtgtt ctgcttcaat cacacatttg acacgttcag cagctagggg tacctgatcg
    43261 ttgacaatcg cataatcata ctcacgcatg agggcaattt cttccttggc cttttcgatt
    43321 cgttgggcaa tcacttctgc actatctgtt ccacgaccta ccaagcgatc ttgcaattca
    43381 tccaaatctg gtggtgtcag gaagataaag acagcatctg gaaccttttt cttgacctga
    43441 agagcaccct gaacttcaat ttcaaggaaa acatcgattc ccttgtccaa ggtttcattg
    43501 acataggtca gaggagttcc atagtagtta ccgacatatt ctgcgtattc caacatctgt
    43561 ccttgacgaa tcagctcttc aaattcttca cgagtacgga agaaatagtc aacaccgtcc
    43621 acttctccag gacgttgtgc gcgtgtcgtc atcgatacag aatattgaaa ttggttttca
    43681 gaactctcaa aaatctctct tctaaccgtt ccttttccaa cccctgaagg accagaaaaa
    43741 acgattagta agcctcggtc tgccattgtg tctcctttta gtcaatctgt gaaataacat
    43801 ttctctagaa taatggcaaa aagccagatt atcctttaca gtctttctat ctagtgtaac
    43861 aaaaaagcag taatttttca actgctcttt cttatttatt tagcataatc tactgcacga
    43921 agctcgcgaa tcacggttac cttgatattt cctggataat cgagattgtt ttcaattttc
    43981 ttacgaactt tgtgagccaa gattgtgact ttgtcgtcct tgatttttcc tggattgacc
    44041 atgatacgaa tttcacgtcc tgcttgaagg gcaaagctag tttgcactcc ttcaaagccg
    44101 ttagcaattt cttccaaatc atggagacgc ttgatgtagc tttcaagaga ctcactacga
    44161 gcacctggac gggctgcgct caaggcatct gctgcagcga cgataactgc tatcacgctc
    44221 tcagcttcaa catctccgtg gtgactagca atcgtattca ccacaactgg gggttccttg
    44281 tacttacggg ccaattccat accgatttca acgtggctac cttcaacctc atggtcaatg
    44341 gctttcccga tatcgtgaag gaatccagca cgacgggcaa gagccgcatt ttcaccaagt
    44401 tcgctcgcca tgataccagc caacttagca acctcaatcg aatggcgcaa aacattttgt
    44461 ccatatgaag tacggaactg caaacgtccc ataatcttca tcaagtctgg atgaaggttt
    44521 ggcgcaccaa tttcataggc agcagcctca ccgtattcac gaatcttatt gtcaatctct
    44581 tgacggtttt tctcaaccaa ctcttcgata cgagctggat gtatacgacc atctttgagc
    44641 aacatttcca tagtcatacg ggcaatctca cgacgaatcg gatcaaatcc tgacaaggtc
    44701 accacttctg gtgtatcgtc gataatcaca tcgacccctg tcaaactttc aaaggtacga
    44761 atgttacgac cttcacgacc aataatgcgt cccttcatag tatcgtctgg cagatgaact
    44821 gttgagtttg ttgactccgc tacatattca ccagcgatac gttgcatagc ttgaaccaag
    44881 atgtccttgg ccattttgtc agaacgttcc ttgacctctt gctcagcttc gcgaatgcga
    44941 ctggcaatct ccctggtcaa gttttcctct gtctgagcca agataatatc tcgtgcttct
    45001 gcctgagaca gcgcaccaat acgctctagt tctgcttctt tttgtctttc gacttcctct
    45061 aattgctctt cacgcgcatc aaggtttttc gctctatcag aaatactttg ttctttttgt
    45121 tcaagtgttt gttctttact cgtcaaattg tcgtccttac ggtcaaggct agtagctctc
    45181 tctgtcaaac gactttcgat ttgtttgagt tcttgacgtt ctgatttgaa ttcagcgtcc
    45241 acttcttcac ggtattttct ggcttcttct ttggcctcca atagtgcttc ttttttaaga
    45301 gacttgcttt cacgtttggc ttcattaaca agtaaatccg cttcacgctc agcttgtcca
    45361 cgtaaattag ttgcttcttg ttcagcattt aaaagcatca actctgcagc ttcctgagat
    45421 gatttcatct tagctgagat gctgacatat ccaatgacta aaccaatgat gacggcaaaa
    45481 acagcaatcg caagcgacat gatttccatg tttttacctc attttattgt tattccgaat
    45541 gacatacatt cttttacatt ctaccataaa aaagtgattt tcacaaacct aaaatagaat
    45601 atgttttgag gaatttggaa cacatttacc aaaataaact tgttgtttag aaatagtagt
    45661 ttagtagaga cttgagaaaa agcctacctt tcaatagact tagtaatgat ctttaaagga
    45721 caagaaagcc acgctatctc catccatcat ataaatcaag cgattttctg catcaatacg
    45781 ccgtgaccag gctccttggt aatcatattt gagtggttct ggtttaccta ttcctgtaaa
    45841 gggatcacgt tgaatatcct tgattagttt attgattctt tttaacgttt tcttatcctg
    45901 attttgccag tagcaataat ctgcccaggc atcttctgta aacttgagca gcatttctta
    45961 ctcctcaata acatggacct gagtacttcc agcacgaact tgagccattc ctcgcaaaac
    46021 cttatcagaa agttccttat tttgagcaat tctcagggtt tcttggatac tatcccactc
    46081 actctttgaa aggactacaa tgtcctcatc tggattttta ttgaccaccg tcaaaggctc
    46141 aaattcatca tttaccttct tcatgtagtc ctttaaatga tttcggaatg ttgagtaaag
    46201 gactgcttcc ataaccatac ctcgttttag ctcttttcca ctattataca cgaaaagaaa
    46261 gaaattgtca ggaacttgta caagattttc ttttctatct atttatactc aatgaaaatc
    46321 aaagagcaaa ctaggaaact agccgcaggc tgtacttgag tacggcaagg cgacgttgac
    46381 gcgatttgaa tttgattttc gaagagtatt attcgtaaaa aatctcaaaa agcctacctt
    46441 tcggtagact tagtttgttt ctattctaat cggcactctt ccaaaatttt gctctgctat
    46501 acttggcttc cctagttggt aaatctggtc tgctttttgg gtcatagcat cccaaggttc
    46561 tttgccaatt cggctgacta gactgacctg acctttcaaa gacttgagat gttgcctacc
    46621 tttttcagta aatccaagga catgaatggc ttctggcaag tcactttctc tagcctgcat
    46681 caaaatatag gtcaataggc gtctgacacg cgccttggtg taacgtttgg tagtcactaa
    46741 ctcgaccaat tcttccacag actgggctgt tttaatagct tctttaatgc gcacagccat
    46801 ttcttgattg acctgataga tggtggttag gtcgggattt gacaagattt gatagcggag
    46861 caagggaaaa tagtcttccc agatcacctt actggcctgc tcaaataggg caacagaagg
    46921 cataaaacgt tctaagaaat cttggtcctt ctgatgttga cggagggctg tcgccgaggc
    46981 aaagtccaca tctttattca cagaatggta acctgccccc tgacgctgaa tcggatgcag
    47041 cttgatattc cgtcctgcaa ccgccttggc ataggccaga gcaagaacat gattgggggt
    47101 attacctgaa aaatcaagac cagcaaattc cttccacata gcttgggttt tctggggata
    47161 ggaaagggaa tcaggcagat tttccacaaa tttctccatc tcagcacctt tctctgtgta
    47221 taagtcagca attttctgat aatctcgaac ttcctctgtc ccaaaaacta gactatcaat
    47281 gcccaagcga tccaagatat ccatagctcc ttgaccaaag aaatcagctg cctgaacact
    47341 gactaaaaag ggcaattcca ctaccaagtc tgcgccattt tccagcgcca tctgggtcct
    47401 tgtccactta tccacaatag caggctctcc acgctgcatg aaattccctg acatggcaac
    47461 aattttcagt ccctctgcct gatccagcag gtatttatgg ccattatgaa aaggattgaa
    47521 ctccgcgata atacctgtta tggtcatgct aatctcctac ttctgcgcca caaaaaacca
    47581 acgggtgcta gtttctgttg gctccttatc ctcaaagtct gcatagagtt tgaaggactt
    47641 aaatccagcc tgttccagca agatatcata ggtcaagatc tcataggtcc tctcctcatg
    47701 tacttcatcg tggcgactaa aggaaccgtc agcctccttg ataaagaaag tcagctcatg
    47761 cacgatggag tgaggaactt cgtcctcata tgtatcccaa agcatggcaa aatcttccgc
    47821 attttcatgg taggaatagc cagggaatac ttcatctgtc tggtaggtcg agtgcacgtc
    47881 aaagatgaaa actccctctt cattcagcgc attgtacacg tccttaaaga cgtcccctac
    47941 ttccacctca tcctgcatat agcagataga gtccgaataa cacgtgacaa aatcgtattt
    48001 ccctgccttg gacaaattca gcatattacc ttctataaaa gcaatctttt gcttggctga
    48061 agttgctctc ttctccgcaa tcttcaacat atccgcactc aagtcaagtc cagtcacatc
    48121 aaaaccagcc tgagagaagc gcactgattg aattcctgtc ccacaagcca attccaagag
    48181 tttctttctc tccttggtct taggcaaatg acgcagagaa aaattcgtcc atttgtcgta
    48241 taaactatcg tccatgaccg catcatagac cgccgcaaag gtttcataag tcgccataat
    48301 catgatacta agagctccat ggtaaacacc actagccctt tacctttcta tcaatttttc
    48361 taaaataagc aaagaaaccc agttgactac aactgagttc atcttctaag caagagcttc
    48421 tgaaatatct actgaattcg cctcatgcca tagcttctct agattataat gggcacgcat
    48481 ttcttctgaa aagatatgca cgacaacagc accgaggtcc agtaagaccc agcctccagc
    48541 tgcatcgcct tcgacatggc tacctttaaa gcctgcttga gctacttttt cacggatatt
    48601 agctgcgata gcgtccaact gacggctatt cattgagcta gtgatgacca agtagtccgt
    48661 cacactagtc aaatcttgta catcaagtgc gaggatatcc tccgcacgtt tctcatcagc
    48721 cgctttcacg actagttcta gtaattcttt ttcgttcatt taatcctctt tttagaaaat
    48781 atgtttgtaa tctagaacat ctgcaaagcg ctcctcccaa atgtcctcat aaaattcatt
    48841 aatcgtttct gctggaatgt catccccatc aaaggttgtg acagcacctt ctggaacaat
    48901 aaactgatag ccatattcaa aggcaacctt gacagaggta tccacacaat attctgtctg
    48961 cataccacat aaaactaatt tttcaatccc ctgcttatcc aagtattctt ttaagccagt
    49021 ttctttgaaa atactgttat acttcttctg aaagaccttt tcagcaggtt ttcgatttaa
    49081 aagcgcagat aactgccaat cttctgatgt ttgagcttca gagttctcaa tatgttgaac
    49141 atagataatt tcaatattct tgcttctagc ctggttttgt aaataagaaa ttttttccaa
    49201 tagacttttt gtctggaacc cagtttctac taaaacattc tgaacatcaa tgattataaa
    49261 agccgtcttc atttagtcct ctttcaaata gtgcacaaag gcgttatagg tttcaagggt
    49321 ttggggatag atggggaatc cctgatgagc tagatactcc acggtacgag ctgtttcgta
    49381 ggccaccgcc ttattgagcg atagacttgc aatctcacgc gccacatcta ctccaggaaa
    49441 ggctcgattg tgctcgatat agtctgcgac gtagatgacc ttatctaggt ctgtcatttg
    49501 accagctccg actgtatgaa tttcaatggc tcgcagaatt tcagaatcat gcaaatccaa
    49561 atcttcctga attttgtaaa ttccgaccat accatgccag acattattgc cccagttttt
    49621 gaggtcaggg tctagctggt aacggtcaat caagactaga aattcctgat ctgacaactt
    49681 tttagcatag tcatgaagaa gacctgctag acttgctttc tcaacatcta caccaaagcg
    49741 ctgagctaat tccatagcgg cacgctccac acctagacaa tgggttagac gtttctcagg
    49801 tagaagctct gccatttttt ccaacaaggc ctcacgggag cagttgatat agtcttgata
    49861 ggacatcagt agagcccttc cttctcgatg tagtctagca ctggctgagg taggagaaag
    49921 ttgggtttcc gaccttgggc aaggaagtcc cgcaccatgc tggacgagat atccatgagc
    49981 ggtacgtcca cccagataac tggataggaa gtccctacct tgtagcgtgg acgctgaacc
    50041 cccacaaact gaaccatgtc aaccagttca tcaattcggt accacttagg cagatagtca
    50101 accatgtcgg caccgatgat aaagtaataa tccgtatctg gattcttctc tgtcaaaatc
    50161 ttcatggtgt cgtaggtgta ggaaataccc ttgcgctcca actcaatggt ttcaatgact
    50221 aggccgtcaa ttccctcaat tgccaactca agcatcttga gacgatggtg ttcagggatg
    50281 gtttcctttt tatcaacgtg aggaggttgg tattcaggca tgagaagaac ttgatccagt
    50341 cccaactgtt gccgtacttg atccgcaaca atgagatggg cattgtgaac agggttaaaa
    50401 ttccccccta aaatcccaac ttgtttgcgt tttttctcct tgatttctgg ctccaactct
    50461 accttggtaa agggagtcaa tagttcgatt gtcataggct agtccccttt atttctctat
    50521 aaaaacagtt atttggagtt tcggtcttag atttctttga ctttcttaga aatcttgcga
    50581 ttttctttct tgctagattg tttaaacaaa atcaagatgc gtcctatttt ttggactgta
    50641 tccacaccga tttcttcttc caaaatttca gctacttcgt ggatgttttc atctgtgttt
    50701 tgtaagagag taaccttgat taattcacgc gcatcaagag cttgacggac gctggttttg
    50761 atttggtcgt tgagtccatt tttcccgatt tggatgatag gtttgagggt gtgtgcctgg
    50821 ctgttgagga aggcacgttg ttttgatgtt aatgacataa ttttcttcct tgttttttga
    50881 tagttatttt aggtggtgag ggacggtcgg aactaatttt ccaaagattt catctttgaa
    50941 aaatggattt cctgacctca caattgagtc ttggtctcaa ttgtgcccct tgccaatcta
    51001 cagattggca atcacaccac agcaataact atcgctatat gagctcactt tgttcgctca
    51061 gcgattttgt tttaaataat tgcttttcgt gtgacgacgg cgacgccttc tggtgcccag
    51121 acggcgactt ttgctatgcc tgttacacga atccatccta ggcctgagat aactaggtct
    51181 gtcttatctt taatggtaaa gacatgctgg actagccttg ggaaatcttc tttttccttg
    51241 ctatttggtg gtgtcagaag agttcccagg tgcttatcgt agaaagcact agctccttca
    51301 agcttgctac gatggagttt gagttcatta tcaaagaaag cagtaaatcc ttgcttttct
    51361 cctgctataa agtcaaagcg tcccaaacca cctaaaaata gggtttgctc aggattaagc
    51421 tgataggtct taggcttgat ttccttttta ggactgacat acttgaggtt tttggccgtc
    51481 aagtagtgag ccatctggtg gcggtggata attcccggcg tatcgtaaat ataagatccg
    51541 tcgtcaagcg gaatctctat tttgtccaag gttgtccctg ggaagcgtga agtagtgatg
    51601 acattctgat cacccgtgat ttcttggata atagcattga ttagagttga ttttccaacg
    51661 ttggtcacac cgaccacata gacatcgcgg cccttacggt agtgttcaat cttgtcaatg
    51721 acttccttaa tggcatgttt attttgtgct gaagttagga ccacatcgac tggacgaaga
    51781 ccttcttcat gggcacgttt catgagccac tggctaatct taccagactt aactgactta
    51841 ggaaggatat cttttttatt tcctaccaag aggacatcat tgcccgagac gaaacgtggt
    51901 aaacctggga tgacagatcc attaaaatca aagatatcaa taacattgac cactaaagca
    51961 tcactgtctc ccacctcgtg caagagcttg aggaaatcat cgttcgtcaa ctggacatct
    52021 gtgatttcat tgtagtggcg gagacggaaa cagcgttggc aatagacttc gccagtctcc
    52081 aaaccttttt caagtgccga ctggggggta aaaccaagac cagctttatc tgtcgtctga
    52141 atggttgctc cacaaccaat acagagaatt tcttccatag ttaaattcct tttttatatg
    52201 taatcggtcc gtacttttca gtgatttttc tcataacacg acgctcacga gttcggttaa
    52261 tctgcgtttt gattgagtca tgttggacca agggtttgac taaaattgac cgaatccctg
    52321 cacggtgggc tgctcgtata tctgtcatga gttggtcacc aaccatgacc acttcctttt
    52381 tgtcatagtg gaattccttc atagcacggt caataccaaa tgtgaagggc ttcaaggccc
    52441 agtaaacgta atcaatccca aatttctcaa ctgctcgttg aacgcgtttt ttggtgttat
    52501 ttgacactac gataatgcca atacccgcgt cccgaaggtc atgtagccat tgcttcatct
    52561 ctggcgttcc atcagggttg ttccaagcaa tgagggtatt atccaaatcg accaaaacag
    52621 cctttattcc ctgcgcctgc aggcttggga ctgtcagatc atagactgct tccacagcaa
    52681 aatctggtat ataattttca atcgccattc tggctccttt tcttaacttt ttttcgcaat
    52741 tttccttagt aacaaggata aaacaatcca caaacctgca ctagccaaac tgatatagag
    52801 ccaagcatgg ggctcatctg ttaaaggtaa gggaacattc attccgaaaa agcctgtcac
    52861 gactgccaaa acagctagca agactgaaat gatagtcaag gttgtcaaat tgtcattcag
    52921 attattgttt agaatattgt tgtaagaggc tgaaagctgc tgtaaaatct gagagattag
    52981 gtctgtcatg gataccagct gatgagcctc aatcatggca tcatcaaact gttctctctc
    53041 aatctcatca aaactacgat acaaggcatg accttgaata tgctctaaca aaatccgatt
    53101 ttgtttggca gctgccgtca gataaaccat accagtctcc aaatcagaca ggacaaagag
    53161 gtttttcttg gtagttcgct ggcgcaagag gtcattgacc tcatccctac tcttgtccat
    53221 ctgctcaatg acaggatagt aggcattgct gatgatttcc agactggcaa agagaaactt
    53281 ataaatcgaa agcgtgtcat ggttctccag ataacgagtc atctgttcaa tgacataggc
    53341 gttcttggta ttactaatgg taatcaggcg acgatgctcg acaataaagg tcatgggaaa
    53401 agtctcatag taggccttgt ccttttttac gtctaagaca ttatagataa aggtaaccgt
    53461 ctcactttca cggtggtagt ccatgtgggc gcgctcgttt ctatccagtg cgtattcaat
    53521 ggtttccttg tccaaaccgt aaatttcaga aaggtcttct agtttattca actttcctag
    53581 gtctaggtct atccaggtac aaccattgcc caactgcttt tctaaaacca tcttttctcc
    53641 ttattaaact ccctctattg taccacaaat tacttaaaat aacaggtcta gtctgtgcgt
    53701 gagaaattgc taacaactgt gatatttcgg ttagggacaa agtgaagggc ttgctcctcg
    53761 ctacgaagct gtgtcgtacg aattcccaca ctgacaacct tacccgatac agtaatcggt
    53821 ccatttgtca gaacgacctc atctcccaca tccagttgac gttcaaagag gatgaaaaag
    53881 ccattgatga catcagacag aaagccttgg gctcccatac caatcgctac cccagcaata
    53941 ccagctccag ccagcaaact agaaactggc aaacctaaaa tcgacaaaat gcagtagagt
    54001 aaaaagaaat atagcgtata attaaacaca ttttctagta aacgtgagat ggttttttgg
    54061 cgtccaacat catgacgaga catttttaga gaaggtttga caattctctg caccatggta
    54121 tgaagcattt ttttagcaat ataaaataca attaaaagca gtaaaagaga aatgaccttg
    54181 gttagaatat tctcgataat ggttgtcaca tctagctttt caatataagc ctgaataaat
    54241 ttttgcatat aaaactcctt tatttcatta taccatattt tcatcagtct cattctccat
    54301 aaatattcaa aaaatagaca gaaaattctt gattttgtct atcatttata ctataatcat
    54361 aatcactaca caaaaaggag attattatga aaagaatcat tcatgcttgg aataaggcaa
    54421 gccttgtcaa gcgtatcttg attggtatgc tttgtgggga atcctaggac tgacccttcc
    54481 taatctctca gggattgggc tactcgagga tttattcgtt ggaggtctaa aagctgttgc
    54541 tcctatccta gtctttgctc tcgttgccaa tgccctttcc caacatcaaa agggacaaga
    54601 tagcaatatg aaaactgttg ttttcttgta tatcctatga tagggacttt cgccgctgct
    54661 cttgtagctg tactagcaaa tttcatcgtc cctattgaaa ttaccccaaa tagtgccaat
    54721 actgaaattg caccaccaga tgggattggg caggttctca gcaacctctt gctcaaactg
    54781 gttgacaacc cagtcaacgc cctgcttact gctaactata ttagaatctt atcttgggca
    54841 gtcatttttg gaatcgctat gagagaagcc agtaaaaata gtcaagaatt gctaaaaact
    54901 atcgctgacg tgacttctaa aattgtcgaa tggatcatca atctggctcc atttggaatc
    54961 cttggtcttg tttttaaaac catttctgac aagggagtcg gaagccttgc caactacggt
    55021 attttattgg ttctattagt aacgactatg ctttttgttg cccctgtggt caaccctttg
    55081 attgccttct tctttatgag acgcaatcct taccctctag tttggaactg cctccgtgtc
    55141 agcggtgtga cagccttttt cactcgtagt tctgcgacta acattcctgt caacatgaaa
    55201 ctctgccatg accttggact caacccagat acctattctg tttctatccc actcggttct
    55261 actatcaata tggctggagt agcgattacc attaaccttt tgacccttgc tgcagttaac
    55321 actcttggaa ttcctgttga ctttgccaca gcctttgtcc tcagtgtggt agcagctatc
    55381 tcatcctgtg atgcttcagg tattgccgga ggttccctcc ttcttatccc agttgcttgt
    55441 agccttttcg gtatttctaa cgatattgcc atacaaattg ttggggttgg ttttgtgatt
    55501 ggtgtcatcc aagactcatg tgaaacagcc cttaactctt ctacagatgt cctctttacc
    55561 gccgttgccg aatacgcagc aacccgtaaa aaataactca tcaaggcaag cctgctcatg
    55621 tcttgtcttt tacgctttta ttctaactta ttaggaaatt cttatgtcta ttagccaacg
    55681 tacgaccaag ctcatcttag ctacctgtct tgcctgcctg cttgcttatt ttctcaatct
    55741 ttcgtcagca gtttcggctg gaattatcgc tctcttgagc ctatctgata cgcgtagaag
    55801 tactttaaaa ctggctcgca atcgtctttt ttctatgctt ctagctctgg ctatcggtgt
    55861 tctagctttt cacttgagcg gatttcatat ctggagtctc ggcctctatc tggccttcta
    55921 cgttccttta gcctacaaga tgggctggga aattggcatc acaccaagca ctgttttggt
    55981 tagccatctc ttggttcaag agtcaacctc tccagacctt ctagtcaatg aattccttct
    56041 ctttgctatt ggtacaggat ttgccttgct tgttaatctc tatatgcctt cacgagaaga
    56101 ggaaatccag cactaccaca cgctggtgga agaaaagtta aaagatatcc tccagcgctt
    56161 caaatactat ttatccagag gagacggacg caaccgagca cagctggtag cagaattaga
    56221 cacgcttttg aaagaagccc tcagactggt ctatttggat cactctgacc acctctttca
    56281 ccagacagac taccatatcc actactttga gatgagacag cgacaaagtc gtatcctgag
    56341 aaacatggcc caacagatta acacttgtca ccttgccgcc agtgaaagcc tgatcttagc
    56401 gcaactcttt tcaaaaattg caggtcaact gagccagacc aatcctgctt ctgatttgct
    56461 agatgaaatt gaacgttatc tggaagtctt ccggaaccgc agtctgccca agacaagaga
    56521 agaatttgaa acccgcgcca cccttcttca actcctacgt gaagccaaaa ccttcatcca
    56581 agtaaaagtt gatttttacc aaaaatatag acagtaatac tcttcaaacc acgtcagcgt
    56641 cgccttgctg tatatatgtt
//
LOCUS       DAVKNL010000005        53786 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVKNL010000005 DAVKNL010000000
VERSION     DAVKNL010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 53786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53786
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.005"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            1..270
                     /locus_tag="VW411_000270"
                     /pseudo
     CDS             1..270
                     /locus_tag="VW411_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            853..1329
                     /locus_tag="VW411_000271"
     CDS             853..1329
                     /locus_tag="VW411_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9793077.1"
                     /translation="MVLDYFFDKNLVFCLEADSQEQLFDQVATLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPITFHNMIAPDK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEALYTYIAEATA"
     gene            1367..1672
                     /locus_tag="VW411_000272"
     CDS             1367..1672
                     /locus_tag="VW411_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9793078.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            1720..3195
                     /locus_tag="VW411_000273"
     CDS             1720..3195
                     /locus_tag="VW411_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEU9793079.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNTAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNIVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            3218..3295
                     /locus_tag="VW411_000274"
                     /pseudo
     CDS             3218..3295
                     /locus_tag="VW411_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            3545..10246
                     /locus_tag="VW411_000275"
     CDS             3545..10246
                     /locus_tag="VW411_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU9793080.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNIVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASNQEKPLKEDAKAVTNEEVNQMIEDRKVDFNQNWYF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLQKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFVYSDGSRAERPVTWSS
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTDLNSVDKSVSYVLID
                     GSVEEYEVDKWEIAEEDKAKLAIPGSRIQATGYLEGQPIHATLVVEEGNPAAPAVPTV
                     TVGGEAVTGLTSQKPMQYRTLAYGAKLPEVTASAKNAAVTVLQASAANGMRASIFIQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLNAEYEGAKDQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQEPSLPATVTVEYDKGFPKTHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRTNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     VPKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKSLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLTVEWEEVPAEN
                     LTKAGQFTVRGRVLGSNLVAEITVRVTDKLGETLSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVTQGKVQFFA
                     DSGTDAPSKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAIKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSFPVLVRLVSESGKQVKEYR
                     IHLTKEKPVSEKTVAAVQEDLPKIEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKVGK
                     ERIFTAINPDGSKEEKLREVVEVPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPSTGSAASQAAVAAGLTLLGLSAGLVVTKGKKED"
     gene            complement(10494..11350)
                     /locus_tag="VW411_000276"
                     /pseudo
     CDS             complement(10494..11350)
                     /locus_tag="VW411_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(11560..11919)
                     /locus_tag="VW411_000277"
     CDS             complement(11560..11919)
                     /locus_tag="VW411_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEU9793081.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSILAIILVFFFLLKKRSFYYPKFIKFFWRAGFL
                     LTLIMYIEMIVELFLMK"
     gene            11942..12520
                     /locus_tag="VW411_000278"
     CDS             11942..12520
                     /locus_tag="VW411_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846832.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEU9793082.1"
                     /translation="METRNRGIYRKVRLLRLWVIFVIMKVMKSYNTLNDYYRKLFGEK
                     TFKVPIDAGFDCPNRDGTVAHGGCTFCTVSGSGDAIVAPDAPIREQFYKEIDFMHRKW
                     PDVRKYLVYFQNFTNTHEKVEVIRERYEQAINEPGVVGINIGTRPDCLPDETIEYLAE
                     LSECMHVTVELGLQTTYEATSDLINRAHSYEL"
     gene            12539..12967
                     /locus_tag="VW411_000279"
     CDS             12539..12967
                     /locus_tag="VW411_000279"
                     /inference="COORDINATES: protein motif:HMM:NF027524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793083.1"
                     /translation="MRKYPKIEIVSHLINGLPGETHEMMVENVRRCVTDNDIQGIKLH
                     LLHLMTNTRMQRDYHEGRLQLMSQDEYVRVICDQLEIIPKHIVIHRITGDAPRDMLLG
                     PMWSLNKWEVLNSIEMEMRRRGSVQGCKAVKQEFENEKTT"
     gene            12951..13508
                     /locus_tag="VW411_000280"
     CDS             12951..13508
                     /locus_tag="VW411_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9793084.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQADLTNAQLILQGHETLDQFVIKAKAGIFNLGYLPAADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            13501..13755
                     /locus_tag="VW411_000281"
     CDS             13501..13755
                     /locus_tag="VW411_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793085.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTHHERAMGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            13767..15821
                     /locus_tag="VW411_000282"
     CDS             13767..15821
                     /locus_tag="VW411_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU9793086.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKNKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            16000..16860
                     /locus_tag="VW411_000283"
     CDS             16000..16860
                     /locus_tag="VW411_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU9793087.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLGFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            17071..17781
                     /gene="ccdA2"
                     /locus_tag="VW411_000284"
     CDS             17071..17781
                     /gene="ccdA2"
                     /locus_tag="VW411_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU9793088.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGFLVLFGNASILSQLFE"
     gene            17801..18367
                     /locus_tag="VW411_000285"
     CDS             17801..18367
                     /locus_tag="VW411_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEU9793089.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            18378..19490
                     /gene="msrB"
                     /locus_tag="VW411_000286"
     CDS             18378..19490
                     /gene="msrB"
                     /locus_tag="VW411_000286"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU9793090.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            19758..20495
                     /locus_tag="VW411_000287"
     CDS             19758..20495
                     /locus_tag="VW411_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9793091.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            20492..22183
                     /locus_tag="VW411_000288"
     CDS             20492..22183
                     /locus_tag="VW411_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9793092.1"
                     /translation="MKRFSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIDTGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYHMKIDSRPQKGTKVEIYINRIETS"
     gene            22299..22862
                     /locus_tag="VW411_000289"
     CDS             22299..22862
                     /locus_tag="VW411_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001006373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEU9793093.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGHIG
                     KESSQEIGELIVETILDILGSRGIHLAVQGCEHVNRALVVERQVAEQFGLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKF"
     gene            22884..28604
                     /locus_tag="VW411_000290"
     CDS             22884..28604
                     /locus_tag="VW411_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9793094.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAGAVDPVATLALASREGVVEMEGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSITNQEQARTENQVVETEEAPKEEAPKTEESPKEEP
                     KSEVKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPAEES
                     KVEQAGEPVAPREDEKAPVEPEKQPEAPEEEKAVEETPKQEESTPDTKAEETVEPKEE
                     TVNQSIEQPKVETPAVEKQTEPTEEPKVEQAGEPVAPREDEQAPTAPVEPEKQPEVPE
                     EEKAVEETPKPEDKIKGIGTKEPVDKSELNNQIDKASSVSPTDYSTASYNALGPVLET
                     AKGVYASEPVKQPEVNSETNKLKTAIDALNVDKTELNNTIADAKTKVKEHYSDRSWQN
                     LQTEVTKAEKVAANTDAKQSEVNEAVEKLTATIEKLVELSEKPILTLTSTDKKILERE
                     AVAKYTLENQNKTKIKSITAELKKGEEVINTVVLTDDKVTTETISAAFKNLEYYKEYT
                     LSTTMIYDRGNGEETETLENQNIQLDLKKVELKNIKRTDLIKYENGKETNESLITTIP
                     DDKSNYYLKITSNNQKTTLLAVKNIEETTVNGTPVYKVTAIADNLVSRTADNKFEEEY
                     VHYIEKPKVHEDNVYYNFKELVEAIQNDPSKEYRLGQSMSARNVVPNGKSYITKEFTG
                     KLLSSEGKQFAITELEHPLFNVITNATINNVNFENVEIERSGQDNIASLANTMKGSSV
                     ITNVKITGTLSGRNNVAGFVNNMNDGTRIENVAFFGKLHSTSGNGSHTGGIAGTNYRG
                     IVRKAYVDATITGNKTRASLLVPKVDYGLTLDHLIGTKALLTESVVKGKIDVSNPVEV
                     GAIASKTWPVGTVSNSVSYAKIIRGEELFGSNDVDDSDYASAHIKDLYAVEGYSSGNR
                     SFRKSKTFTKLTKEQADAKVTTFNITADKLESDLSPLAKLNEEKAYSSIQDYNAEYNQ
                     AYKNLEKLIPFYNKDYIVYQGNKLNKEHHLNTKEVLSVTAMNNNEFITNLDEANKIIV
                     HYADGTKDYFNLSSSSEGLSNVKEYTITDLGIKYTPNIVQKDNTTLVNDIKSILESVE
                     LQSQTMYQHLNRLGDYRVNAIKDLYLEESFTDVKENLTNLITKLVQNEEHQLNDSPAA
                     RQMIRDKVEKNKAALLLGLTYLNRYYGVKFGDVNIKELMLFKPDFYGEKVSVLDRLIE
                     IGSKENNIKGSRTFDAFGQVLAKYTKSGNLDAFLNYNRQLFTNIDNMNDWFIDATEDH
                     VYIAERASEVEEIKNSKHRAFDNLKRSHLRNTILPLLNIDKAHLYLISNYNAIAFGSA
                     ERLGKKSLEDIKDIVNKAADGYRNYYDFWYRLASDNVKQRLLRDAVIPIWEGYNAPGG
                     WVEKYGRYNTDKVYTPLREFFGPMDKYYNYNGTGAYAAIYPNSDDIRTDVKYVHLEMV
                     GEYGISVYTHETTHVNDRAIYLGGFGHREGTDAEAYAQGMLQTPVTGSGFDEFGSLGI
                     NMVFKRKNDGNQWYITDPKTLKTREDINRYMKGYNDTLTLLDEIEAESVISQQNKDLN
                     SAWFKKIDREYRDNNKLNQWDKIRNLSQEEKNELNIQSVNDLVDQQLMTNRNPGNGIY
                     KPEAISYNDQSPYVGVRMMTGIYGGNTSKGAPGAVSFKHNAFRLWGYYGYENGFLGYA
                     SNKYKQQSKTDGESVLSDEYIIKKISNNTFNTIEEFKKAYFKEVKDKATKGLTTFEVN
                     GSSVSSYDDLLTLFKEAVKKDAETLKQEANGNKTVSMNNTVKLKEAVYKKLLQQTNSF
                     KTSIFK"
     gene            28665..30386
                     /gene="pabB"
                     /locus_tag="VW411_000291"
     CDS             28665..30386
                     /gene="pabB"
                     /locus_tag="VW411_000291"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEU9793095.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDIVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLNANPFAIYNRMVVEQEA
                     GYNAYVEHDEMTVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGKISQKQLMFEE
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANRDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQHLLETGQVEEKVLTLADLTQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            30387..31004
                     /locus_tag="VW411_000292"
     CDS             30387..31004
                     /locus_tag="VW411_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9793096.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALTLELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKTLMVGVSEELKTLDLTDIAGTYPYPTLLICGSKDKPNL
                     SSMKALHRLLTDSQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            31054..32052
                     /locus_tag="VW411_000293"
     CDS             31054..32052
                     /locus_tag="VW411_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9793097.1"
                     /translation="MNKRLFSKMSLVTLPILALFSQSVLAEENIHFSSCKEAWANGYS
                     DIHEGEPGYSAKLDRDHDGVACELKNAPKGAFKAKQSTAIQINTSSATTSGWVKQDGA
                     WYYFDGNGNLVKNAWQGSYYLKADGKMAQSEWIYDSSYQAWYYLKSDGSYAKNAWQGA
                     YYLKSNGKMAQGEWVYDSSYQAWYYLKSDGSYARNAWQGNYYLKSDGKMAKGEWVYDA
                     TYQAWYYLTSDGSYAYSTWQGNYYLKSDGKMAVNEWVDGGRYYVGADGVWKEVQASTA
                     SSSNDSNSEYSAALGKAKSYNSLFHMSKKRMYRQLTSDFDKFSNDAAQYAIDHLDD"
     gene            32179..33138
                     /locus_tag="VW411_000294"
     CDS             32179..33138
                     /locus_tag="VW411_000294"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEU9793098.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            33235..34074
                     /locus_tag="VW411_000295"
     CDS             33235..34074
                     /locus_tag="VW411_000295"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEU9793099.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(34122..34292)
                     /locus_tag="VW411_000296"
     CDS             complement(34122..34292)
                     /locus_tag="VW411_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEU9793100.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            34413..35297
                     /gene="miaA"
                     /locus_tag="VW411_000297"
     CDS             34413..35297
                     /gene="miaA"
                     /locus_tag="VW411_000297"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEU9793101.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEGIHNRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVDQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQETLYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTLEEASESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            35290..36528
                     /gene="hflX"
                     /locus_tag="VW411_000298"
     CDS             35290..36528
                     /gene="hflX"
                     /locus_tag="VW411_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEU9793102.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            36521..37144
                     /locus_tag="VW411_000299"
     CDS             36521..37144
                     /locus_tag="VW411_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEU9793103.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTDQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGLGRIFFENEEKISDDLLFEIAQIFPHQLVFEESGKIYMKPVEDEEVISLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            37159..38088
                     /locus_tag="VW411_000300"
     CDS             37159..38088
                     /locus_tag="VW411_000300"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEU9793104.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTGASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            38109..38864
                     /locus_tag="VW411_000301"
     CDS             38109..38864
                     /locus_tag="VW411_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU9793105.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEPDAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(38918..39826)
                     /locus_tag="VW411_000302"
     CDS             complement(38918..39826)
                     /locus_tag="VW411_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU9793106.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            39890..40159
                     /locus_tag="VW411_000303"
     CDS             39890..40159
                     /locus_tag="VW411_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEU9793107.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            40159..40539
                     /locus_tag="VW411_000304"
     CDS             40159..40539
                     /locus_tag="VW411_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9793108.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            40628..40855
                     /locus_tag="VW411_000305"
     CDS             40628..40855
                     /locus_tag="VW411_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793109.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLDYKIEKILNILGIVISVVNWIVAIALIFR"
     gene            complement(40895..41620)
                     /locus_tag="VW411_000306"
     CDS             complement(40895..41620)
                     /locus_tag="VW411_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEU9793110.1"
                     /translation="MRLDNLLAQEKISRKAMKQALLRGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGAVTANKDKELPTVMDLLPSNIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQTFQKGIVFLDDTVC
                     KPAKLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     EGNYRPLNQKELQIIKNYLEMSR"
     gene            41782..43623
                     /gene="typA"
                     /locus_tag="VW411_000307"
     CDS             41782..43623
                     /gene="typA"
                     /locus_tag="VW411_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEU9793111.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGIKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            43643..43897
                     /locus_tag="VW411_000308"
     CDS             43643..43897
                     /locus_tag="VW411_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEU9793112.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            44261..44560
                     /locus_tag="VW411_000309"
                     /pseudo
     CDS             44261..44560
                     /locus_tag="VW411_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
     gene            44631..46652
                     /locus_tag="VW411_000310"
     CDS             44631..46652
                     /locus_tag="VW411_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9793113.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLSYKALVPVIKGR
                     LPLKRLMALTLLCQLVAVFTVGYAVKTGLTSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASLFILSSRDLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            46654..47295
                     /locus_tag="VW411_000311"
     CDS             46654..47295
                     /locus_tag="VW411_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9793114.1"
                     /translation="MIELKNISKKFGSRQLFSDMNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            47399..48751
                     /locus_tag="VW411_000312"
     CDS             47399..48751
                     /locus_tag="VW411_000312"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEU9793115.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLTSKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            48753..49811
                     /locus_tag="VW411_000313"
     CDS             48753..49811
                     /locus_tag="VW411_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEU9793116.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKCGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLAKVEHVGA
                     VTKVSDKNTPEPDELVDIQTHFNPKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELRQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            49821..51020
                     /gene="divIB"
                     /locus_tag="VW411_000314"
     CDS             49821..51020
                     /gene="divIB"
                     /locus_tag="VW411_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEU9793117.1"
                     /translation="MSKDKKNEDKETLEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEEPES
                     KEKEEQDKKLAKKATKEKPAKAKIPGIHILRAFTILFPSLLLLFVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVVDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNRNQT
                     TQRSSRR"
     gene            51779..52415
                     /locus_tag="VW411_000315"
                     /pseudo
     CDS             51779..52415
                     /locus_tag="VW411_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824573.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M50 family protein"
     gene            52399..53393
                     /locus_tag="VW411_000316"
                     /pseudo
     CDS             52399..53393
                     /locus_tag="VW411_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397972.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
     gene            53390..53659
                     /locus_tag="VW411_000317"
                     /pseudo
     CDS             53390..53659
                     /locus_tag="VW411_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        16741 a         9696 c        11646 g        15703 t
ORIGIN      
        1 caaaactagg aagggcaatt gaatacagcc tcaagtatga agaaaccttt aagactattt
       61 tgaaagacgg acatctggtc ctttccaata atctagctga acgcgccatt aaatcattgg
      121 ttatgggacg gagtaaaaga gtccagtgga ctcttttagc ctgagctcag tttaaaaaag
      181 cgagggtggt tattttctca aagttttgaa ggagctaaag caagagctat tgttatgagc
      241 ttgttggaaa cagctaaacg tcatcaatta tagtgcgttg aatctataac agtacgcatc
      301 gactgctaaa acatttctat aaatcaattt tcctttccta atcgatttgt tcatatctta
      361 tttcaatcca ttataaatag cgagaaatat ctatcctatc ttctagaatg tcttccaaac
      421 gaggaaactc tcgtaaacaa agaggtttta gaggtttatt taccatggac taaagttgta
      481 caagaaaagt gcaaataaga aatctccaga ttaggaacta tccgtgagtt cactaatctg
      541 gagatttttc aatagacttc gttattgggc ggttacgata tgatcactga cttcgtcagt
      601 cttatctaca acctcaaaac agtgttttga gcaacctgcg actagcttcc tagtttactc
      661 tttgattttc attgaatatt agaacagaaa aaatgcttgg agtatttgtt tgtgtgttta
      721 tttttatata acaaactata aacaaaataa aaatataaaa aaagagacaa aaaagaacag
      781 aaagtaattg acaacggttt catatagtgt tatacttatg gcataaagtt aatggaaaga
      841 agggaaaaac ttatggtatt agattatttc tttgacaaaa accttgtgtt ttgcttagaa
      901 gcggatagtc aagaacaact ctttgatcag gttgcaactt tattggaaga acgagaaatt
      961 gtcactccaa cttatcgtga agccttgatc acgcgtgaaa agtcatttcc aactggttta
     1021 gatatggaat ttctaggaaa ggacttgcca aatgtagcga ttcctcacac agatattgtt
     1081 cataatctag ctgagaaagt ggtggttgtt cgattagaaa aaccaataac ttttcacaat
     1141 atgatagctc ctgataagga agtagaagta tccctactct tctttatcat taataattca
     1201 agttcaagtc aaacgaacat tcttgctcag ttaatggact tcttcacagg aaatggtcat
     1261 cttgaagacc tatcaaaaat ttctgaacca gaagcccttt atacttacat tgctgaagca
     1321 accgcttaat cttgtctatt aaaaaataaa atcggaggaa atctaaatga ttaaaattct
     1381 tgctgcctgc ggtgcaggtg ttaactcaag tcaccaaatt aaaagtgctc tagaagaaga
     1441 actttcaaac cgtggttatg atgttcactg tgatgcagtc atggtgaaag atgtaaacga
     1501 agaccttatg aaaggttacg atatctttac accaatcgct gcaacagacc ttggttttga
     1561 accaggtatc ccagttatcg aagctgggcc aatcttattc cgtatcccag caatgagcgc
     1621 tccagtattt gacaatattg aagcagctat taaagaacac ggattaagct aattctaatt
     1681 tgttaacatt catataacat caaaaaggga ggaactgtta tggatgcaat ctttgaccta
     1741 atcggaaagg ttttcaatcc catcttagaa atgggtggac ctgtcatcat gttaatcatt
     1801 ttgacagtat tggctttact ttttggagtg aaattctcca aagcgcttga aggtggtatc
     1861 aaacttgcca tcgctcttac aggtatcggt gctatcatcg gtatgctaaa cactgctttc
     1921 tcagcatcac tagcaaaatt cgttgaaaac actggtatcc aattgagtat taccgacgtt
     1981 ggttgggcac cacttgctac aatcacttgg ggttctgctt ggacactata cttcttgctc
     2041 atcatgttga ttgtcaacat agtgatgcta gctatgaaga aaacagatac acttgatgtc
     2101 gatatctttg atatctggca cttgtctatc acaggtctct tgattaaatg gtatgctgat
     2161 aacaatggtg tgagtcaagg ggtttcactc tttattgcta cagcagctat cgtccttgtc
     2221 ggtgtgttga aaattatcaa ctctgacttg atgaaaccta catttgatga ccttcttaac
     2281 gccccaagtt catcaccaat gacatcaact cacatgaact acatgatgaa cccagttatc
     2341 atggttttgg ataagatttt tgaaaaattc ttcccaggcc ttgataaata tgactttgat
     2401 gctgctaaat tgaacaagaa aatcggtttc tggggatcta aattcttcat cggtttcatc
     2461 cttggtatcg ttatcggtat tatgggaact ccacatccaa ttgcaggtgt tgcagatgca
     2521 gataaatggc gtcttgttat caaaggatgg ttgtctcttg gtttgactgc cggtgtatct
     2581 ttggaactct tctcacttat cggttcatgg ttcatcgcag ccgtagaacc actatcacaa
     2641 ggtattacaa acgttgctac taaacgtctt caaggacgta aattcaatat cggtcttgac
     2701 tggccattca tcgctggtcg tgctgaaatc tgggcttgtg ccaacgtact tgcaccaatc
     2761 atgttgattg aagcagtgct tctttcaaaa gttggaaatg gtatcttgcc acttgcaggt
     2821 atcatcgcta tgggtgttac tccagctctc ttggttgtaa ctcgtggtaa attgctccgt
     2881 atgattatct tcggaacact cttgttgcca ctcttccttc tttcaggtac acttattgca
     2941 ccatttgcaa cagaacttgc taaaggtgta ggtgccttcc cagaaggtgt gagccaaact
     3001 caattgatta ctcactctac tcttgaagga ccaatcgaaa aacttcttgg ttggacaatt
     3061 ggtaacacta caactggtga tatcaaagca atccttggtg cagtagtctt ccttgtattc
     3121 tatatcggta tctttgcttg gtacagaaaa caaatgatca aacgtaacga agagtacgca
     3181 gcaaaagcaa aataatgcgc tcctataaaa tataaactca atgaaaatca aagagcaaac
     3241 taggaagcta gccgcaggtt gctcaaaaca cagttttgag gttgtagata agactgacga
     3301 agtcagctca aaacatggtt ttgaggttgt agatgaaact gacgaagtca gctcaaaaca
     3361 tggttttgag gttgtagatg aaactgacga agtcagtaat catatctacg gcaaggcgac
     3421 actgacgaag tttgaattgg attttcgcag agtgtaaatt gttgaaacta ggttgtcata
     3481 ccatgtatac cacttgggct ttggtagtag ctaactgcac taaatataat ataaggaggg
     3541 ttttatgggg aaaggccatt ggaatcggaa aagagtttat agcattcgta agtttgctgt
     3601 gggagcttgc tcagtaatga ttgggacttg tgcagtttta ttaggaggaa atatagctgg
     3661 agaatctgta gtttatgcgg atgaaacact tattactcat actgctgaga aacctaaaga
     3721 ggaaaaaatg atagtagaag aaaaggctga taaagctttg gaaactaaaa atatagttga
     3781 aaggacagaa caaagtgaac ctagttcaac tgaggctatt gcatctgaga agaaagaaga
     3841 tgaagccgta actccaaaag aggaaaaagt gtctgctaaa ccggaagaaa aagctccaag
     3901 gatagaatca caagcttcaa atcaagaaaa accgctcaag gaagatgcta aagctgtaac
     3961 aaatgaagaa gtgaatcaaa tgattgaaga caggaaagtg gattttaatc aaaattggta
     4021 ctttaaactc aatgcaaatt ctaaggaagc cattaaacct gatgcagacg tatctacgtg
     4081 gaaaaaatta gatttaccgt atgactggag tatctttaac gatttcgatc atgaatctcc
     4141 tgcacaaaat gaaggtggac agctcaacgg tggggaagct tggtatcgca agactttcaa
     4201 actagatgaa aaagacctca agaaaaatgt tcgccttact tttgatggcg tctacatgga
     4261 ttctcaagtt tatgtcaatg gtcagttagt ggggcattat ccaaatggtt ataaccagtt
     4321 ctcatatgat atcaccaaat accttcaaaa agatggtcgt gagaatgtga ttgctgtcca
     4381 tgcagtcaac aaacagccaa gtagccgttg gtattcagga agtggtatct atcgtgatgt
     4441 gactttacaa gtgacagata aggtgcatgt tgagaaaaat gggacaacta ttttaacacc
     4501 aaaacttgaa gaacaacaac atggcaaggt tgaaactcat gtgaccagca aaatcgtcaa
     4561 tacggacgac aaagaccatg aacttgtagc cgaatatcaa atcgttgaac gaggtggtca
     4621 tgctgtaaca ggcttagttc gtacagcgag tcgtacctta aaagcacatg aatcaacaag
     4681 cctagatgcg attttagaag ttgaaagacc aaaactctgg actgttttaa atgacaaacc
     4741 tgccttgtac gaattgatta cgcgtgttta ccgtgacggt caattggttg atgctaagaa
     4801 ggatttgttt ggttaccgtt actatcactg gactccaaat gaaggtttct ctttgaatgg
     4861 tgaacgtatt aaattccatg gagtatcctt gcaccacgac catggggcgc ttggagcaga
     4921 agaaaactat aaagcagaat atcgccgtct caaacaaatg aaggagatgg gagttaactc
     4981 catccgtaca acccacaacc ctgctagtga gcaaaccttg caaatcgcag cagaactagg
     5041 tttactcgtt caggaagagg cctttgatac gtggtatggt ggcaagaaac cttatgacta
     5101 tggacgtttc tttgaaaaag atgccactca cccagaagct cgaaaaggtg aaaaatggtc
     5161 tgattttgac ctacgtacca tggtcgaaag aggcaaaaac aaccctgcta tcttcatgtg
     5221 gtcaattggt aatgaaatag gtgaagctaa tggtgatgcc cactctttag caactgttaa
     5281 acgtttggtt aaggttatca aggatgttga taagactcgc tatgttacca tgggagcaga
     5341 taaattccgt ttcggtaatg gtagcggagg gcatgagaaa attgctgatg aactcgatgc
     5401 tgttggattt aactattctg aagataatta caaagccctt agagctaagc atccaaaatg
     5461 gttgatttat ggatcagaaa catcttcagc tacccgtaca cgtggaagtt actatcgccc
     5521 tgaacgtgaa ttgaaacata gcaatggacc tgagcgtaat tatgaacagt cagattatgg
     5581 aaatgatcgt gtgggttggg ggaaaacagc aaccgcttca tggacttttg accgtgacaa
     5641 cgctggctat gctggacagt ttatctggac aggtacggac tatattggtg aacctacacc
     5701 atggcacaac caaaatcaaa ctcctgttaa gagctcttac tttggtatcg tagatacagc
     5761 cggcattcca aaacatgact tctatctcta ccaaagccaa tgggtttctg ttaagaagaa
     5821 accgatggta caccttcttc ctcactggaa ctgggaaaac aaagaattag catccaaagt
     5881 agctgactca gaaggtaaga ttccagttcg tgcttattcg aatgcttcta gtgtagaatt
     5941 gttcttgaat ggaaaatctc ttggtcttaa gactttcaat aaaaaacaaa ccagcgatgg
     6001 gcggacttac caagaaggtg caaatgctaa tgaactttat cttgaatgga aagttgccta
     6061 tcaaccaggt accttggaag caattgctcg tgatgaatct ggcaaggaaa ttgctcgaga
     6121 taagattacg actgctggta agccagcggc agttcgtctt attaaggaag accatgcgat
     6181 tgcagcagat ggaaaagact tgacttacat ctactatgaa attgttgaca gccaggggaa
     6241 tgtggttcca actgctaata atctggttcg cttccaattg catggccaag gtcaactggt
     6301 cggtgtagat aacggagaac aagccagccg tgaacgctat aaggcgcaag cagatggttc
     6361 ttggattcgt aaagcattta atggtaaagg tgttgccatt gtcaaatcaa ctgaacaagc
     6421 agggaaattc accctgactg cccactctga tctcttgaaa tcgaaccaag tcactgtctt
     6481 tactggtaag aaagaaggac aagagaagac tgttttgggg acagaagtgc caaaagtaca
     6541 gaccattatt ggagaggcac ctgaaatgcc taccactgtt ccgtttgtat acagtgatgg
     6601 tagccgtgca gaacgtcctg taacctggtc ttcagtagat gtgagcaagc ctggtattgt
     6661 aacggtgaaa ggtatggctg acggacgaga agtagaagct cgtgtagaag tgattgctct
     6721 taaatcagag ctaccagttg tgaaacgtat tgctccaaat actgacttga attctgtaga
     6781 caaatctgtt tcctatgttt tgattgatgg aagtgttgaa gagtatgaag tggacaagtg
     6841 ggagattgcc gaagaagata aagctaagtt agcaattcca ggttctcgta ttcaagcgac
     6901 cggttattta gaaggtcaac caattcatgc aacccttgtg gtagaagaag gcaatcctgc
     6961 ggcacctgca gtaccaactg taacggttgg tggtgaggca gtaacaggtc ttactagtca
     7021 aaaaccaatg caataccgca ctcttgctta tggagctaag ttgccagaag tcacagcaag
     7081 tgctaaaaat gcagctgtta cagttcttca agcaagcgca gcaaacggca tgcgtgcgag
     7141 catctttatt cagcctaaag atggtggccc tcttcaaacc tatgcaattc aattccttga
     7201 agaagcgcca aaaattgctc acttgagctt gcaagtggaa aaagctgaca gtctcaaaga
     7261 agaccaaact gtcaaattgt cggttcgagc tcactatcaa gatggaacgc aagctgtatt
     7321 accagctgat aaagtaacct tctctacaag tggtgaaggg gaagtcgcaa ttcgtaaagg
     7381 aatgcttgag ttgcataagc caggagcagt cactctgaac gctgaatatg agggagctaa
     7441 agaccaagtt gaactcacta tccaagccaa tactgagaag aagattgcgc aatccatccg
     7501 tcctgtaaat gtagtgacag atttgcatca ggaaccaagt cttccagcaa cagtaacagt
     7561 tgagtatgac aaaggtttcc ctaaaactca taaagtcact tggcaagcta ttccgaaaga
     7621 aaaactagac tcctatcaaa catttgaagt actaggtaaa gttgaaggaa ttgaccttga
     7681 agcgcgtgca aaagtctctg tagaaggtat cgtttcagtt gaagaagtca gtgtgacaac
     7741 tccaatcgca gaagcaccac aattaccaga aagtgttcgg acatatgatt caaatggtca
     7801 cgtttcatca gctaaggttg catgggatgc gattcgtcca gagcaatacg ctaaggaagg
     7861 tgtctttaca gttaatggtc gcttagaagg tacgcaatta acaactaaac ttcatgttcg
     7921 cgtatctgct caaactgagc aaggtgcaaa catttctgac caatggaccg gttcagaatt
     7981 gccacttgcc tttgcttcag actcaaatcc aagcgaccca gtttcaaatg ttaatgacaa
     8041 gctcatttcc tacaataacc aaccagccaa tcgttggaca aactggaatc gtactaatcc
     8101 agaagcttca gtcggtgttc tgtttggaga ttcaggtatc ttgagcaaac gctccgttga
     8161 taatctaagt gtcggattcc atgaagacca tggagttggt gtaccgaagt cttatgtgat
     8221 tgagtattat gttggtaaga ctgtcccaac agctcctaaa aaccctagtt ttgttggtaa
     8281 tgaggaccat gtctttaatg attctgccaa ctggaaacca gttactaatc taaaagcccc
     8341 tgctcaactc aaggctggag aaatgaacca ctttagcttt gataaagttg aaacctatgc
     8401 tgttcgtatt cgcatggtta aagcagataa caagcgtgga acgtctatca cagaggtaca
     8461 aatctttgcg aaacaagttg cggcagccaa gcaaggacaa acaagaatcc aagttgacgg
     8521 caaagactta gcaaacttca accctgattt gacagactac taccttgagt ctgtagatgg
     8581 aaaagttccg gcagtcacag caagtgttag caacaatggt ctcgctaccg tcgttccaag
     8641 cgttcgtgaa ggtgagccag ttcgtgtcat cgcgaaagct gaaaatggcg acatcttagg
     8701 agaataccgt ctgcacttca ctaaggataa gagcttactt tctcataaac cagttgctgc
     8761 ggttaaacaa gctcgcttgc tacaagtagg tcaagcactt gaattgccga ctaaggttcc
     8821 agtttacttc acaggtaaag acggctacga aacaaaagac ctgacagttg aatgggaaga
     8881 agttccagcg gaaaatctga caaaagcagg tcaatttact gttcgaggcc gtgtccttgg
     8941 tagtaacctt gttgctgaga tcactgtacg agtgacagac aaacttggtg agactctttc
     9001 agataaccct aactatgatg aaaacagtaa ccaggccttt gcttcagcaa ccaatgatat
     9061 tgacaaaaac tctcatgacc gcgttgacta tctcaatgac ggagatcatt cagaaaatcg
     9121 tcgttggaca aactggtcac caacaccatc ttctaatcca gaagtatcag cgggtgtgat
     9181 tttccgtgaa aatggtaaga ttgtagaacg gactgttaca caaggaaaag ttcagttctt
     9241 tgcagatagt ggtacggatg caccatctaa actcgtttta gaacgctatg tcggtccaga
     9301 gtttgaagtg ccaacctact attcaaacta ccaagcctac gacgcagacc atccattcaa
     9361 caatccagaa aattgggaag ctgttcctta tcgtgcggat aaagacattg cagctggtga
     9421 tgaaatcaac gtaacattta aagctatcaa agccaaagct atgagatggc gtatggagcg
     9481 taaagcagat aagagcggtg ttgcgatgat tgagatgacc ttccttgcac caagtgaatt
     9541 gcctcaagaa agcactcaat caaagattct tgtagatgga aaagaacttg ctgatttcgc
     9601 tgaaaatcgt caagactatc aaattaccta taaaggtcaa cggccaaaag tctcagttga
     9661 agaaaacaat caagtagctt caactgtggt agatagtgga gaagatagct ttccagtact
     9721 tgttcgcctc gtttcagaaa gtggaaaaca agtcaaggaa taccgtatcc acttgactaa
     9781 ggaaaaacca gtttctgaga agacagttgc tgctgtacaa gaagatcttc caaaaatcga
     9841 atttgttgaa aaagatttgg catacaagac agttgagaaa aaagattcaa cactgtatct
     9901 aggtgaaact cgtgtagaac aagaaggaaa agttggaaaa gaacgtatct ttacagcgat
     9961 taatcctgat ggaagtaagg aagaaaaact ccgtgaagtg gtagaagttc cgacagaccg
    10021 catcgtcttg gttggaacca aaccagtagc tcaagaagct aaaaaaccac aagtgtcaga
    10081 aaaagcagat acaaaaccaa ttgattcaag tgaagctagt caaactaata aagcccagtt
    10141 accaagtaca ggtagtgcgg caagccaagc agcagtagca gcaggtttaa ctcttctagg
    10201 tttgagtgca ggattagtag ttactaaagg taaaaaagaa gactagaaat tttgatacct
    10261 tctttatcat taaagaccaa agaaaaactc ttgtcctaaa tagaagataa agagcctgag
    10321 acaaaatagt tctcaaccac aaaaaagcta gatatttcca attgtggaac tctagctttt
    10381 taattttgag tcattctctt attgtatagt agattgaaac tagaatagta cacttttact
    10441 tctaaaacat tgttagaaat cgatttgatt gtccttatcg atttgtcctg ttcttatttc
    10501 attttactat attttaggag gttattggcc ataagtacca atcccatgtc aattctcact
    10561 tgacgcttcc ctctcagatt acatctcttg taacccaaag aagcctttat ctgcccaaag
    10621 acaggttcca catcaatctt gcgttgagcg aaaatctgtc taccttgggg agataaaagc
    10681 gcctgacatt ctttagcttt caagttttga tagcgttcgt tcatatacag tcccttttga
    10741 ggggctgatt caggttcgtc ggcgtagtaa accttgattt cttgttgaaa gtctgtctgt
    10801 gttttctgat atttggtatg gtgaaaacga taataccagc catcaggatg tgtgtagcta
    10861 tcctccttgt cattatagtg ccaattcgct aagtttttag ctgactcttt ataccctctc
    10921 ttttgtttct tatcaaacat ggcatattta atcagaaggg gagtgatttc ttcttgaaaa
    10981 tagacctgta tctgttcttg aagtttggcg gagaatttct ctgtcgtttt cttccacaca
    11041 aaactatact tgttggcatt ggcttcaatc ttagttctgt caataaacaa acaatctaag
    11101 gtcactaact cttccatttt tcaatttttc gaccagagaa aatcccttgt gaataggcaa
    11161 atagaagagt agatacaagc attttagggt gataagacgg gcgatcaaag gcatgatagg
    11221 aggtgtagaa gtgacgttcc tccaaggtat tcaccacttt ttcaatagta aaaacgagat
    11281 gatcttgtgg taagaaggaa ctgatttcta gtggtaaagt tgtttgattt gtgttatagt
    11341 gaatatgcat gggatagcct ttctaaatgg tttattgggc aattctattt taccaagact
    11401 atcgtaacca tccaaaaaag caacggcaaa tccgttgcct ttttggatat aagagactat
    11461 tgtcccagac tcttttcttt gtataaaatt gtatagtgtt attgtctata ctattttgta
    11521 gagaaagcaa aaatccctag aaaatgctta gggattcgac tatttcatta agaacaattc
    11581 aacaatcatt tctatataca tgataagggt taataagaat cctgcacgcc agaagaattt
    11641 gataaattta gggtagtaaa agctgcgttt tttcaaaagg aagaaaaaga cgagaataat
    11701 ggctaggatc gagagggcta gccccagtct agggaggaaa ttatgggtaa aggttttagc
    11761 tgtaatcaag taatactcaa ataccaagac tggaaaagcc aaatccgcaa agtttcgtcc
    11821 tagttttttt aatctaaaaa gtttggttag gataatgcag acgactaagg tcagtatcaa
    11881 taataaaata gatgctaatt tcattaaaat catacccata ttgtatcata aaaaaaggct
    11941 aatggaaacg agaaacagag gaatttatag gaaagtcagg cttttgagat tgtgggtgat
    12001 ttttgttata atgaaagtca tgaaatctta taataccttg aatgattatt atcgaaaact
    12061 ctttggagaa aagactttta aagtccctat tgatgcggga tttgattgtc ccaatcgtga
    12121 tgggactgtg gctcatgggg gctgtacttt ttgtacggtt tctggttctg gagatgccat
    12181 tgtggcaccg gatgcgccta tccgtgagca attttataag gaaattgact ttatgcaccg
    12241 caagtggccg gatgttcgga agtatctggt ttattttcaa aattttacca acacccatga
    12301 aaaggtggaa gttattcgag agcgttatga acaggctatc aatgagccag gtgtggtagg
    12361 aatcaatatt ggaacgcgcc cagactgttt accagacgaa accatcgaat atttggctga
    12421 gttatcggag tgcatgcatg tgacggttga attgggcttg caaactactt atgaagcaac
    12481 ctctgacctg attaaccgtg cgcattctta tgaattgtag gtggaaacgg tcaagcgttt
    12541 gagaaagtat ccaaaaattg agattgtttc ccatttgatc aatggtttgc ctggtgaaac
    12601 ccatgagatg atggttgaaa atgtccgccg ttgtgtcacg gataacgata ttcaagggat
    12661 taaactgcat ttgctccatc ttatgacaaa tactcgtatg caacgagatt accatgaggg
    12721 gcgtttacaa ttgatgagtc aggacgaata tgtcagggtc atctgtgacc aactggaaat
    12781 tattcccaaa catatcgtca tccatcgaat tacaggagat gcgcctaggg atatgctgct
    12841 tggtcctatg tggagcctca ataaatggga agtcctcaac agcattgaga tggagatgcg
    12901 acgtcgtgga agtgttcaag gatgcaaggc tgtaaaacag gagtttgaaa atgaaaagac
    12961 cacttgagat ggcacatgat tttttggctg aggtcgtgac aaaagaggat gtcgtagtgg
    13021 atgcgactat gggaaatggt catgacacgc tttttttagc caagctagcc aagcaagtct
    13081 atgcctttga tattcagaag caagccttgg aaaagaccca agagcgtttg catcaggctg
    13141 acttgacaaa tgcccagtta atcttgcaag gccatgagac actggaccag tttgtgataa
    13201 aagctaaggc agggattttt aatctgggct atttgccggc agctgataag tctgtcatca
    13261 cccgaccgca gacaacgatt gaggcattag aaaagctatg tggcttactt gtcaaaggtg
    13321 gacgaattgc tattatgatt tactatggtc atgaaggagg cgatctcgag agagatgctg
    13381 tcttggattt tgtgatccag ttgaaccaac aagagtacac agctgccatt taccgaactt
    13441 taaaccaagt caacaacccg ccgtttttag tgatgattga aaaattagag agatacagac
    13501 atggataaac aatacttaca cgaaaagctg gatgccatgc gccagaattt tgttgaatca
    13561 acccaccacg aacgagcgat gggcgtgcta gaccaagcgc atatgagcaa aaaaatgctt
    13621 aaaatcaaga aaaaattagt tgctcttgaa atggaacggt gccagagaaa aattgagcac
    13681 aaagactgtt ccaagattga ccaaaaaatc aaagagcaga aggagatatt tgaatcctgt
    13741 tgtaaaaaag attaaggagg acgtgcgtgg aattactgat ttacctcatc ctatttttac
    13801 tggttttgat tgtctcaagt acaaccaata agctcctgcc ctttttgcct ctccctttgg
    13861 tgcaaattct tttgggaatt gtgattggtc tctttttacc caatactgac tttcacctta
    13921 atacggagtt gtttttggca ctggttatcg gacccttgct tttccgagag gctgaagaag
    13981 cagatgttac ggctatttta aaacactggc gaatcattgt ttatctcata tttccagtga
    14041 tttttatctc gaccctgagt ttgggtggct tggcccatct tctttggttc agccttccat
    14101 tggcagcttg cttggctgtt ggggcagccc ttggtcctac ggacttggtg gcctttgcct
    14161 ctctttcgga gcgttttagc tttcctaagc gcgtgtccaa tattcttaag ggcgaaggac
    14221 tcttgaatga tgcttctggt ttggtggctt ttcaggtagc tttgacagct tggacaactg
    14281 gagctttttc tctggggcaa gctagcagtt cgctcatctt ttcaatccta ggcggttttt
    14341 taattggatt tttaacagcc atgaccaacc gcttcctcca taccttcttg ctaagtgtgc
    14401 gcgcaacgga tattgccagt gaacttttat tagaattgag tttgcctcta gtgaccttct
    14461 ttctggcaga agaagttcat gtttcaggta ttattgccgt cgtagttgct ggaattttaa
    14521 aggcaagtcg cttcaagaaa atcacgctcc tcgaagccca agtggatacg gtgaccgaga
    14581 cggtctggca tacagtgacc tttatgctca acggttctgt ctttgtgatt ttagggatgg
    14641 agttggaaat gatagcagaa cctatcttga ccaatccaat ctataatcct ctacttttat
    14701 tgctatctct tatcgccctt acctttgtcc tttttgtcat tcgttttatt atgatctatg
    14761 gctattatgc ctatagaacc cgacgcctca agaaaaagct aaataagtat atgaaggaca
    14821 tgtttctctt gaccttttca ggtgttaagg gaacggtgtc gattgctacg attctcttga
    14881 taccaagtaa tctagaacag gagtatcctc ttttgctttt ccttgttgca ggtgtgacgc
    14941 ttgttagctt tttaacaggt ctcttggtct tgcctcatct ttctgatgaa gaggaagaaa
    15001 gcaaggatta tcttatgcat atcgccattt tgaatgaagt aacgctagag ttggaaaaag
    15061 agttggaaga caccagaaat aaacttcccc tctatgcggc tattgacaat tatcatggac
    15121 gtattgaaaa tctcatttta agccaagaaa accaggatga tcaagaagac tgggctgctt
    15181 tgaaactctt gattcttagt attgaaagtg atggtttgga acaggcctat gaagagggga
    15241 acattagcaa tcgtgcttac cgagtttacc aacgttatct gaaaaatata gaacaaggaa
    15301 tcaatcgtaa acttgcctca agactgacct attattttct tgtttccttg aggattttac
    15361 gttttctcct tcatgaagtt tttactctcg gaaagacctt ccgtagctgg aagaacaagg
    15421 agcaaagccg tctccgtgct cttgattatg accaaattgc agagctctat cttgccaata
    15481 cagagatgat tattgaaagt ttggaaaacc tgaagggagt ctacagacgc tctttgatta
    15541 gttttatgca ggagtctcgt cttcgagaaa cagctattat cagcagtggt gcctttgtcg
    15601 aacgggttat caatcgtgtc aaacccaaca atatcgatga aatgctgaga ggctattatc
    15661 tggagcgcaa gttgattttc gaatacgaag aaaaacgatt gattacgact aagtatgcca
    15721 agaaattacg acaaaatgta aataacttag agaactattc cttgaaggaa gctgccaata
    15781 ccctaccgta tgatatggtg gaattggtaa gaagaaatta gttaatactc ttcgaaaatc
    15841 tcttcaaacc acgtcagcgt cgccttaccg tatgtatgtg actgacttcg tcagtcttat
    15901 ctacaacctc aaaacagtgt tttgagcagc ttgcggctag ctttctagtt tgctctttga
    15961 ttttcattga gtataagatt ataagtgaag gagtatgaca tgaaaaagtg gtggaaagag
    16021 ctgatagaca aacctttatt aaaagctttt ttgcattatt atcaagcatc agatagtgag
    16081 ttgaccagtg tcgcagtagc ctactattgg ttgatttcga ttttccccct gcttttggtg
    16141 gtggtcaata tcctccccta ttttcagatt cctgtgggag aatttttggg ctttatgaaa
    16201 gacgtcctgc ccccaagcct ctatgaaggt gtggaaaaaa tagccagaga agtcttgacg
    16261 caaccttcaa caggtttatt gagtttttct gttttatcgg cgttatggag tttttcaaaa
    16321 tctatgaatt tcttgcaaaa agcttttaac aaggcttatg gcgtcgaaaa gagtcgtgga
    16381 cttatttccc atcagatgct aagtctccta gtcagttttg gcttgcagct cctctttgcg
    16441 tttgccttgt ttttgatctt atttggtcag atgattttgg ctttacttgc tcattattgg
    16501 acaaaagatg gcattatcta tcaggcttta caagggttgg caggtcctct gatttacgcc
    16561 ctcctctttg ctatcttggt catgctttat tattttcttc ccaatctatc taacaggaaa
    16621 attagctata ccctaccagg cagtgccttt gttctcttag tgattttagg tttactaact
    16681 ctgttctcta gttatctcaa ttactatgtc catcatttgg tggatgtccg aattttaggt
    16741 tccgttcttc ttgttgttat gatgttttgg tttatcttga ttgctaaaat tgttatccta
    16801 ggcgcggtga tcaatgccag tatgcagagt ttgaaagatc cggtctttaa aaaagactaa
    16861 ctatttatcc attacaaaag cgcatagtat caaggcttta tagaatcttg atactatgcg
    16921 cttttgtgct ttgacagttt tttgtatagt ttttgtccaa gatttctaaa tcatctgatt
    16981 aaattcctat tttcccctat cattgtccct gttttttcga aaatggaagg agtataataa
    17041 atctatcaat aaaatgatag gaggaacttt atgggtcata ttttcttttt tctaagtgtc
    17101 tttttggcag ggattctatc cttcttttct ccttgtatct tacctttgtt accggtctat
    17161 acaggagtgt tactagatga taaggatggt gctcaggctt ctagcggcaa attttcaatc
    17221 tcagttacta gtttattacg aactctggcc tttatagcag gaatttcctt tatatttatt
    17281 ttgttgggct atggagctgg ttttttaggc gatttgcttt atgcttcttg gttccaatat
    17341 cttactgggg caattattat ccttcttggt ttgcaccaaa tggagattct acactttaag
    17401 gggctttata aggaaaagag gctacaactg caaggacagg ggcaaaatgg taagggctat
    17461 agtcaggcat ttttattggg cttgaccttt agttttgctt ggacgccttg cgtggggccg
    17521 gttctggggt ctgttttggc cttggcggct tcaggtggtt caggagcttg gcagggagct
    17581 ggtctcatgt tggtgtatac gctgggcttg gcgctaccat tcttgcttct agctctgacc
    17641 tctagttatg ttttgaaaca tttccgaaaa cttcatccct atctcggaat cctcaaaaaa
    17701 gtgggtggtt ttctcattat tgtgatgggc ttcttggttc tgtttggaaa tgcttcaatt
    17761 ttaagtcaat tatttgaata aaatggaaag gaatatcaat atgaaaaaat ggcaaacatg
    17821 tgttcttgga gcaggttcgc tcctttgttt gacggcttgt tcaggcaagt ccgtgactag
    17881 tgaacaccaa acgaaagatg aaatgaagac ggagcagaca gctagtaaaa caagcgcagc
    17941 taaagggaaa gaggtggctg attttgaatt gatgggagta gatggcaaga cctaccgttt
    18001 atctgattac aagggcaaga aagtctatct caaattctgg gcttcttggt gttccatctg
    18061 tctggctagt cttccagata cggatgagat tgctaaagaa gctggtgatg actatgtggt
    18121 cttgacagta gtgtcaccag gacataaggg agagcaatct gaagcggact ttaagaattg
    18181 gtataaggga ttggattata aaaatctccc agtcctagtt gacccatcag gcaaactttt
    18241 ggaaacttat ggtgtccgtt cttacccaac ccaagccttt atagacaaag aaggcaagct
    18301 ggtcaaaaca catccaggat tcatggaaaa agatgcaatt ttgcaaactt tgaaggaatt
    18361 agcctaggag gcgtcttatg aatgataagt taaaaatctt cttgttgcta ggagtatttt
    18421 ttctagccat aaccggtttc tatgttctat tgatacgaaa tgcagggcag acagatgcct
    18481 cgcaaattga aaaggcggca gttagccaag gaggaaaagc agtgaaaaaa acagaaatta
    18541 gtaaagacgc agacttgcac gaaatttatc tagctggagg ttgtttctgg ggagtggagg
    18601 aatatttctc acgtgttccc ggggtgacgg atgccgtttc aggctatgca aatggtagag
    18661 gagaaacaac caagtacgaa ttgattaacc aaacaggtca tgcagaaacc gtccatgtca
    18721 cctatgatgc caagcaaatt tctctcaagg aaatcctgct tcactatttc cgcattatca
    18781 atccaaccag caaaaataaa caaggaaatg atgtggggac ccagtaccgt actggtgttt
    18841 attacacaga tgacaaggat ttggaagtga ttaaccaagt ctttgatgag gtggctaaga
    18901 aatacgatca acctctagca gttgaaaagg aaaacttgaa gaattttgtg gtggctgagg
    18961 attaccatca agactatctc aagaaaaatc caaatggcta ctgccatatc aatgttaatc
    19021 aggcggccta tcctgtcatt gatgccagca aatatccaaa accaagtgat gaggaattga
    19081 aaaagaccct gtcacctgag gagtatgcag ttacccagga aaatcaaaca gaacgagctt
    19141 tctcaaaccg ttactgggat aaatttgaat ccggtatcta tgtggatata gcaactgggg
    19201 aacctctctt ttcatcaaaa gacaaatttg agtctggttg tggctggcct agttttaccc
    19261 aacccatcag tccagatgtt gtcacctaca aggaagataa gtcctacaat atgacgcgta
    19321 tggaagtgcg gagccgagta ggagattctc accttgggca tgtctttacg gatggtccac
    19381 aggacaaggg cggcttacgt tactgtatca atagcctctc tatccgcttt attcccaaag
    19441 accaaatgga agaaaaaggc tacgcttatt tactagatta tgttgattaa tactcttcga
    19501 aaatctcttc aaaccacgtc agcgtcgcct taccgtatgt agggttactg acttcgtcag
    19561 ttttatctac aacctcaaag ctatactttg agctgacttc gtcagtttta tttacaacct
    19621 caaagcggta ctttgagaaa cctgtggcta tcttcctagt ttactttttg atttttattg
    19681 agtataagag gtctttccta agcagttaga ggagaatttt gctatactga tactagtaag
    19741 tggtaaagga gcagagcatg acctacacaa tcttaatcgt agaagatgaa tatctggtaa
    19801 gacaaggttt gactaaactg gtcaatgtag cagcctacga tatggaaatc atcggtcagg
    19861 ctgaaaatgg aaggcaggct tgggaattga tccaaaagca ggtgccagat atcattttaa
    19921 ccgatatcaa catgcctcat ctaaatggca tccagttggc cagtctggta cgagaaacct
    19981 atcctcaggt tcatttggtc tttttaacag gttacgatga ttttgattat gccttgtctg
    20041 ctgtcaaact aggtgtggac gactacctgc tcaaaccctt ttctcgtcag gatattgagg
    20101 aaatgttggg gaaaatcaaa caaaaactag acaaggaaga gaaagaagag cagttacaag
    20161 atttattgac taataggttt gaaggaaaca tggcccagaa aatccagtct catctggctg
    20221 atagccaatt tagtttaaag tctttagcca gtgacttagg ttttagtccg acctatctga
    20281 gttccttgat taagaaagag ttgggcttgc cttttcagga ttatctggtg agagaacgtg
    20341 ttaaacaagc caagctcttg cttttaacta cagatctgaa gatttatgag atcgcagaga
    20401 aggttggttt tgaagatatg aactatttta cccaacgttt taagcagatt gcaggtgtga
    20461 cacctcgtca gtttaagaag ggagaagacc gatgaagcgt ttttctcttt tagttagaat
    20521 ggttatttcc atctttctgg tctttctcat tctcctagct ctggttggaa ctttctacta
    20581 tcaatcaagt tcttcagcca ttgaggccac cattgagggc aacagccaaa cgaccatcag
    20641 ccagactagc cactttattc agtcttatat caaaaaacta gaaaccacct cgactggttt
    20701 gacccagcag acggatgttc tggcctatgc tgagaatccc agtcaagaca aggtcgaggg
    20761 aatccgagat ttgtttttga ccatcttgaa gtcagataag gacttgaaaa ctgttgtgct
    20821 ggtgaccaaa tctggtcagg tcatttctac agatgacagt gtgcagatga aaacttcctc
    20881 tgatatgatg gctgaggatt ggtaccaaaa ggccattcat cagggagcta tgcctgtttt
    20941 gactccagct cgtaaatcag atagtcagtg ggtcatttct gtcactcaag aacttgttga
    21001 tgcaaaggga gccaatcttg gtgtgcttcg tttggatatt tcttatgaaa ctctggaagc
    21061 ctatctcaat caactccagt tggggcagca gggctttgcc ttcattatca atgaaaacca
    21121 tgaatttgtc taccatcctc aacacacagt ttatagttcg tctagcaaaa tggaggctat
    21181 gaaaccctac atcgatacag gtcagggtta tactcctggt cacaaatcct acgtcagtca
    21241 agagaagatt gcaggaactg attggacggt gcttggcgtg tcatcattgg aaaagttaga
    21301 ccaggttcgg agtcagctct tgtggacctt gcttggggcc agtgtcacat ctcttcttgt
    21361 ctgtctctgc ttagtgtggt tcagtcttaa acgctggatt gctcctttga aggatttgag
    21421 agaaaccatg ttggaaattg cttctggtgc tcaaaatctt cgtgccaagg aagttggtgc
    21481 ctatgaactg agagaagtaa ctcgccaatt taatgctatg ttggatcaga ttgatcagtt
    21541 gatggtagct attcgtagcc aggaagaaac gacccgtcag taccaacttc aagccctttc
    21601 gagccagatt aatccacatt tcctctataa cactttggac accatcatct ggatggctga
    21661 atttcatgat agtcagcgag tggtgcaggt gaccaagtcc ttggcaacct atttccgctt
    21721 ggcgctcaat caaggcaagg acttgatttg tctctctgac gaaatcaatc atgtccgcca
    21781 gtatctcttt atccagaaac aacgctatgg agataagctg gaatacgaaa ttaatgaaaa
    21841 tgttgccttt gataatttag tcttacccaa gctggtccta caaccccttg tagaaaatgc
    21901 tctttaccat ggcattaagg aaaaggaagg tcagggccat attaaacttt ctgtccagaa
    21961 acaggattcg ggattggtca tccgtattga ggatgatggc gttggcttcc aagatgctgg
    22021 tgatagtagt caaagtcaac tcaaacgtgg gggagttggt cttcaaaatg tcgatcaacg
    22081 gctcaaactt cattttggag ccaattacca tatgaagatt gattctagac cccaaaaagg
    22141 gacgaaagtt gaaatatata taaatagaat agaaactagc taactcccag tcaattctgg
    22201 gagttttatg cgtaattggg caaactttct aggttgtcta tcttgctaaa aacaagaaaa
    22261 aacggtatga tagaagagtg acaacagagg taacagagat gaacgagaca cagattcaaa
    22321 gagaaacacg tcaggtagtg gaagatgttt tagaaaagac caatttgaag cagggggccc
    22381 tttttgtttt aggtctatct tctagtgagg tgctaggtgg tcatattggg aaagaatcca
    22441 gtcaagagat tggtgagctg atagtggaga ctatcttgga tattctaggt agtagaggta
    22501 tccatttagc agttcaaggt tgtgagcatg tcaatcgggc tctcgttgtt gaacgtcagg
    22561 tggcagagca gtttggtctg gaaattgtca gtgtccatcc tactcttcat gcaggaggtt
    22621 cggggcagtt ggcagccttt aagtttatgc aggatccagt tgaggttgaa tttatcaagg
    22681 ctcatgctgg attggatatc ggagacactg caattggcat gcatgtcaag catgttcagg
    22741 ttccgattcg ccctattttg agagagattg gtcatgccca tgtaacggca ctggctagtc
    22801 gtccaaaatt aatcggaggt gcgcgtgcgc actatccgca agatgctatt aggaagtttt
    22861 gagaatgaat aaaggagata aaagtgttta aaaaagaccg tttttcaatt cgtaagatta
    22921 agggagttgt aggctctgta tttcttggaa gccttttgat ggctccttct gtagtggacg
    22981 cagccaccta tcactatgta aataaagaga ttatttcaca agaagctaaa gatttaattc
    23041 agacaggaaa gcctgacagg aatgaagttg tatatggttt ggtgtatcaa aaagatcagt
    23101 tgcctcaaac agggacagaa gcatctgttt tgacagcttt tggtttgctg actgttggga
    23161 gcttgctttt aatctacaag agaaagaaaa ttgctagcgt ctttctagtt ggagctatgg
    23221 gattggtagt tcttcctagt gcaggggctg tagacccagt tgcgacccta gcgctggcta
    23281 gtcgagaggg tgttgttgaa atggagggct atcgctatgt tggttatcta tcaggtgaca
    23341 tcctcaaaac gcttggcttg gacactgttt tagaagaaac ctcagcaaaa cctggagagg
    23401 tgactgtggt cgaagttgag actcctcaat caataacaaa tcaggagcaa gctaggacag
    23461 aaaaccaagt agtagagaca gaggaagctc caaaagaaga agcacctaaa acagaagaaa
    23521 gtccaaagga agaaccaaaa tcggaggtaa aacctactga cgacaccctt cctaaagtag
    23581 aagaggggaa agaagattca gcagaaccag ctccagttga agaagtaggt ggagaagttg
    23641 agtcaaaacc agaggaaaaa gtagcagtta agccagaaag tcaaccatca gacaaaccag
    23701 ctgaggaatc aaaagttgaa caagcaggtg aaccagtcgc gccaagagaa gacgaaaagg
    23761 caccagtcga gccagaaaag caaccagaag ctcctgaaga agagaaggct gtagaggaaa
    23821 caccgaaaca agaagagtca actccagata ccaaggctga agaaactgta gaaccaaaag
    23881 aggagactgt taatcaatct attgaacaac caaaagttga aacgcctgct gtagaaaaac
    23941 aaacagaacc aacagaggaa ccaaaagttg aacaagcagg tgaaccagtc gcgccaagag
    24001 aagacgaaca ggcaccaacg gcaccagttg agccagaaaa gcaaccagaa gttcctgaag
    24061 aagagaaggc tgtagaggaa acaccgaaac cagaagataa aataaagggt attggtacta
    24121 aagaaccagt tgataaaagt gagttaaata atcaaattga taaagctagt tcagtttctc
    24181 ctactgatta ttctacagca agttacaatg ctcttggacc tgttttagaa actgcaaaag
    24241 gtgtctatgc ttcagagcct gtaaaacagc ctgaggtaaa tagcgagaca aataaactta
    24301 aaacggctat tgacgctcta aacgttgata aaactgaatt aaacaatacg attgcagatg
    24361 caaaaacaaa ggtaaaagaa cattacagtg atagaagttg gcaaaacctc caaactgaag
    24421 ttacaaaggc tgaaaaagtt gcagctaata cagatgctaa acaaagtgaa gttaacgaag
    24481 ctgttgaaaa attaactgca actattgaaa aattggttga attatctgaa aagccaatat
    24541 taacattgac tagtaccgat aagaaaatat tggaacgtga agctgttgct aagtatactc
    24601 tagaaaatca aaacaaaaca aaaatcaaat caatcacagc tgaattgaaa aaaggagaag
    24661 aagttattaa tactgtagtc cttacagatg acaaggtaac aacagaaact ataagcgctg
    24721 catttaagaa cctagagtac tacaaagaat acaccctatc tacaactatg atttacgaca
    24781 gaggtaacgg tgaagaaact gaaactctag aaaatcaaaa tattcaatta gatcttaaaa
    24841 aagttgagct taaaaatatt aaacgtacag atttaatcaa atacgaaaat ggaaaagaaa
    24901 ctaatgaatc actgataaca actattcctg atgataagag caattattat ttaaaaataa
    24961 cttcaaataa tcagaaaact acattactag ctgttaaaaa tatagaagaa actacggtta
    25021 acggaacacc tgtatataaa gttacagcaa tcgcagacaa tttagtctct agaactgctg
    25081 ataataaatt tgaagaagaa tacgttcact atattgaaaa acctaaagtc cacgaagata
    25141 atgtatatta taatttcaaa gaattagtgg aagctattca aaacgatcct tcaaaagaat
    25201 atcgtctggg acaatcaatg agcgctagaa atgttgttcc taatggaaaa tcatatatca
    25261 ctaaagaatt cacaggaaaa cttttaagtt ctgaaggaaa acaatttgct attactgaat
    25321 tggaacatcc attatttaat gtgataacaa acgcaacgat aaataatgtg aattttgaaa
    25381 atgtagagat agaacgttct ggtcaagata atattgcatc attagccaat actatgaaag
    25441 gttcttcagt tattacaaat gtcaaaatta caggcacact ttcaggtcgt aataatgttg
    25501 ctggatttgt aaataatatg aatgatggaa ctcgtattga aaatgttgct ttctttggca
    25561 aactacactc tacaagtgga aatggctctc atacaggggg aattgcaggt acaaactata
    25621 gaggaattgt tagaaaagca tatgttgatg ctactattac aggaaacaaa acacgcgcca
    25681 gcttgttagt tcctaaagta gattatggat taactctaga ccatcttatt ggtacaaaag
    25741 ctctcctaac tgagtcggtt gtaaaaggta aaatagatgt ttcaaatcca gtagaagttg
    25801 gagcaatagc aagtaagact tggcctgtag gtacggtaag taattctgtc agctatgcta
    25861 agattatccg tggagaggag ttattcggct ctaacgacgt tgatgattct gattatgcta
    25921 gtgctcatat aaaagattta tatgcggtag agggatattc gtcaggtaat agatcattta
    25981 ggaaatctaa aacatttact aaattaacta aagaacaagc tgatgctaaa gttactactt
    26041 tcaatattac tgctgataaa ttagaaagtg atctatctcc tcttgcaaaa cttaatgaag
    26101 aaaaagccta ttctagtatt caagattata acgctgaata taaccaagcc tataaaaatc
    26161 ttgaaaaatt aataccattc tacaataaag attatattgt atatcaaggt aataaattaa
    26221 ataaagaaca ccatctaaat actaaagaag ttctttctgt taccgcgatg aacaacaatg
    26281 agtttatcac aaacctagat gaagctaata aaattattgt tcactatgcg gacggtacaa
    26341 aagattactt taacttgtct tctagcagtg aaggtttaag taatgtaaaa gaatatacta
    26401 taactgactt aggaattaaa tatacaccta atatcgttca aaaagataac actactcttg
    26461 ttaatgatat aaaatctatt ttagaatcag tagagcttca gtctcaaacg atgtatcagc
    26521 atctaaatcg attaggtgac tatagagtta atgcaatcaa agatttatat ttagaagaaa
    26581 gcttcacaga tgttaaagaa aacttaacaa acctaatcac aaaattagtt caaaacgaag
    26641 aacatcaact aaatgattct ccagctgctc gtcaaatgat tcgtgataaa gtcgagaaaa
    26701 acaaagcagc tttattacta ggtttaactt acctaaatcg ttactatgga gttaaatttg
    26761 gtgatgttaa tattaaagaa ttaatgctat tcaaaccaga tttctatggt gaaaaagtta
    26821 gcgtattaga cagattaatt gaaatcggtt ctaaagagaa caacattaaa ggttcacgta
    26881 cattcgacgc attcggtcaa gtattggcta aatatactaa atcaggtaat ttagatgcat
    26941 ttttaaatta taatagacaa ttgttcacaa atatagacaa tatgaacgat tggtttattg
    27001 atgctacaga agaccatgtc tacatcgcag aacgcgcttc tgaggtcgaa gaaattaaaa
    27061 attctaaaca tcgtgcattc gataatttaa aacgaagtca ccttagaaat actatactcc
    27121 cactactgaa tattgataaa gcacatcttt atttaatttc aaattataat gcaattgcct
    27181 ttggtagtgc agagcgatta ggtaaaaaat cattagaaga tattaaagat atcgttaaca
    27241 aagctgcaga tggttataga aactattatg atttctggta tcgtctagcg tctgataacg
    27301 ttaaacaacg actactaaga gatgctgtta ttcctatttg ggaaggttat aacgctcctg
    27361 gtggatgggt tgaaaaatat ggccgctata ataccgacaa agtatatact cctcttagag
    27421 aattctttgg tcctatggat aagtattata attataatgg aacaggagct tatgctgcta
    27481 tatatcctaa ctctgatgat attagaactg atgtaaaata tgttcattta gaaatggttg
    27541 gtgaatacgg tatttcagtt tacacacatg aaacaacaca cgtcaacgac cgtgcgattt
    27601 acttaggtgg ctttggacac cgtgaaggta ctgatgctga agcatatgct cagggtatgc
    27661 tacaaactcc tgttactggt agtggatttg atgagtttgg ttctttaggt attaatatgg
    27721 tatttaaacg caaaaatgat gggaatcagt ggtatattac agatccaaaa actctaaaaa
    27781 cacgagaaga tattaataga tatatgaagg gttataatga cactttaact cttcttgatg
    27841 aaattgaggc tgaatctgtg atttctcaac aaaataaaga tttaaatagt gcatggttca
    27901 aaaaaataga tagagaatac cgtgataaca ataaattaaa tcaatgggat aaaattcgaa
    27961 atctaagtca agaagagaaa aatgaattaa atattcaatc tgttaatgat ttagttgatc
    28021 aacaattaat gactaatcgc aatccaggta atggtatcta taaacccgaa gcaattagct
    28081 ataacgatca atcaccttat gtaggtgtta gaatgatgac cggtatctac ggaggtaata
    28141 ctagtaaagg tgctcctgga gctgtttcat tcaaacataa tgcttttaga ttatggggtt
    28201 actacggata cgaaaatggg ttcttaggtt atgcttcaaa taaatataaa caacaatcta
    28261 aaacagatgg tgagtctgtt ctaagtgatg aatatattat caagaaaata tctaacaata
    28321 catttaatac tattgaagaa tttaaaaaag cttacttcaa agaagttaaa gataaagcaa
    28381 cgaaaggatt aacaacattc gaagtaaatg gttcttccgt ttcatcatac gatgatttac
    28441 tgacattgtt taaagaagct gttaaaaaag atgccgaaac tcttaaacaa gaagcaaacg
    28501 gtaataaaac agtatctatg aataatacag ttaaattaaa agaagctgtt tataagaaac
    28561 ttcttcaaca aacaaatagc tttaaaactt caatctttaa ataattgaaa tctctcatct
    28621 gctttgcgga tgagggtttt cttattttat gctatactta aggtatgcat agaaaaacag
    28681 tgattgattt taggactttg ggggagagat atacctttac ccagcctatt aaagagttga
    28741 aaacgagaaa tgtagaagaa gtggcagatt tgctggcaca agtggaaagc taccaagagc
    28801 aaggttatta tgtggtgggg tatgtcagct acgaggctgc acctgctttt gaggaaaaat
    28861 tagcagttca caaggatccc ctactgggag agtacctgct ttactttact gttcatgata
    28921 tcgtagaaac ttcccctatt cctctgactt atgaggatat tgatttgccc tcaaattggc
    28981 aggaagtaac gtctgcagca gactatgaaa aggccattgc ccaaattcac catcatttac
    29041 ggcagggaga tacctatcag gtcaactaca ccgtccaact taagcaaaag ttaaatgcca
    29101 atccttttgc catctacaat cgtatggtgg tagagcagga ggcgggctac aatgcctatg
    29161 tggaacatga cgagatgaca gtgatttcca tgagcccaga gctctttttt gagcaaaatg
    29221 atcgcgagtt gacaacacga ccaatgaagg ggacgactca gcgtggggta actgaccaag
    29281 aagatcttga acaggccagt tggttggaac aggatcccaa aaatcgctct gaaaatatga
    29341 tgattgtgga cctcttgcgc aatgatatga accgtatttc tgaagttggg agcgagcacg
    29401 tggagcgtct gtgtcaggta gagcagtatt caactgtttg gcagatgact tcgaccatca
    29461 agagtcagtt gcgagaggat gtggaccttg ttgaaatctt ccgctcactc tttccttgtg
    29521 gttccataac gggtgcaccg aaaattgcga caatggagat tatcaaggac ttggagcctc
    29581 aaccgcgtgg agtctactgt ggaacgattg gtctcttgct tccaaatgga cgacggattt
    29641 ttaatgtggc cattcgtacc attcaacttc acaaaggtca agccatttat ggagttggcg
    29701 gagggattac ttgggatagc acatgggaat ctgaataccg agaggttcat caaaaggcag
    29761 cagttctcta tcgtaaacaa gctcgcttcc aattaattac aactggaaaa atcagccaaa
    29821 aacaactgat gtttgaagaa caacatctgg aaaggctgag aaaagctagt cgttattttg
    29881 cttttccttt tgatgcagaa gacttgggac acaagattga ggaagagtgt caggattgtg
    29941 aagctaatcg agattaccgc ttgcgaatca gccttagcaa atctggagag atagaagtca
    30001 atcgtcaagt attaacccct ctcagtacaa gcttttgtca ggcccaagtc tgtcttcagg
    30061 aagctgcttt gaatcaatcc tttacctact ttaaaaccac tcaccgaccg catttgagcc
    30121 taggagaaca agagaagatt taccacaata agtcaggaga actgcttgaa acctctatag
    30181 gaaatttggt tctgaaaatc gctggaaaac tctacacacc gcctatccga cttggaatct
    30241 tgccaggaat ttaccgtcag catttgctag aaacaggaca ggtagaagag aaagtcttga
    30301 ccttggcaga tttaacccaa gcagaagcta tttacggctg taatgcagtg agaggcttgt
    30361 atgaattaag ccttgaggag aactagatga aattaatatt tttgcatggg ttagggcagt
    30421 cagcagagtc ttggaaagaa gttcggaatc tactgacaga ttatccttct gaggctatcg
    30481 agttatttcc ttctggagtt agtaactatc aacaagctaa agagcgagtt tatcagcacc
    30541 tagctcaaga gacagaacct tttgttttga ttggattgtc cttaggtgct gccctgacac
    30601 tagaactttc cagttacgat ttaccaaatc ttcgagcttt gattctgtca ggatgcccgc
    30661 ttaaattagc agggaatatc ctattttatc ttcaattgct gatttttaaa ctacttccca
    30721 aaagagtatt tgaaaaacag ggagcagaca agactcttat ggtcggagtt tctgaggagt
    30781 tgaagacact tgatttaacg gatatagcag gaacttaccc ttatccaacc ttactaattt
    30841 gtggcagcaa agataaaccg aatcttagtt ctatgaaagc tctccataga ctgttaacag
    30901 attcgcagtt tcagatcatt cctgatggcc ctcatgtctt gaataaggca aaaccaaagg
    30961 agtttgtaga aaagaccaga agtttccttg aattgctgaa ataagtttga taaaataata
    31021 ttgaaatcga tatcataatt atgggagaaa gaaatgaata aacgtctatt ttcaaaaatg
    31081 agtctggtga cgttgccaat tttagccttg ttttcacaat cagttttggc ggaagaaaac
    31141 atccattttt cgagctgtaa ggaagcttgg gcgaatggct attcggatat tcacgaggga
    31201 gaacctggtt attctgccaa gttagaccgt gatcatgatg gtgtggcttg cgaattgaaa
    31261 aatgctccta agggtgcttt taaagcaaaa cagtcaacgg ctattcaaat caacacaagt
    31321 tcagcaacaa caagtggttg ggttaagcag gacggcgctt ggtactactt tgatggaaat
    31381 ggaaatctag tgaaaaatgc atggcaggga agctattacc tgaaagctga tggtaaaatg
    31441 gcacagagtg aatggattta tgactcttct tatcaagctt ggtattattt gaaatcagat
    31501 ggttcttatg caaaaaatgc atggcaagga gcttattacc ttaaatcaaa cggtaaaatg
    31561 gcacaaggtg agtgggttta tgattcttct taccaagcat ggtattactt gaaatcagat
    31621 ggttcatatg ctcgcaatgc atggcaagga aactactatt tgaaatcaga tggtaaaatg
    31681 gctaaaggtg aatgggttta tgatgccacc tatcaagctt ggtattattt gacatcagat
    31741 ggttcttatg cttacagtac atggcaagga aattactatc taaaatcgga tggtaaaatg
    31801 gctgtcaatg aatgggttga tggtggacgt tattatgttg gcgctgacgg agtttggaag
    31861 gaagttcaag caagtacagc ttcttctagt aatgatagca atagtgaata ttctgctgct
    31921 ttaggaaagg caaaaagtta taattcgtta ttccacatgt caaaaaaacg tatgtataga
    31981 caattaactt ctgattttga taaattttca aatgatgcag ctcaatatgc cattgatcat
    32041 ttagatgatt aacagatact ggtaggcgaa aaaattcctt ataattcatt cacgcaaacc
    32101 tttgcataag tgaatggatt ttgatatact agtactgtaa gcattttcaa ttactacaca
    32161 atgaaaaagg agaatatgat gagtcaaaag attattggga ttgaccttgg tggaacttct
    32221 atcaaatttg caatcttaac aacagcagga gaaatccaag gaaaatggtc aatcaagacc
    32281 aacattttgg atgagggaag tcatatcgtt gatgatatga ttgagtctat tcaacatcgt
    32341 ttggacttgc ttggattggc agcagcggac ttccaaggca ttggaatggg atcaccaggt
    32401 gtggttgacc gtgacaaagg gactgttatc ggtgcctaca acttgaactg gaaaaccctt
    32461 caaccaatca aacaaaagat tgaaaaagct ttgggcattc catttttcat cgataatgat
    32521 gccaacgtag cagctcttgg tgagcgctgg atgggtgctg gagataacca accagacgtt
    32581 gtctttatga cactcggtac tggtgttggt ggtggtattg tcgcagaagg caaattgctt
    32641 catggtgttg ctggtgcagc aggtgaactt ggtcatatca ctgttgactt tgaccaacca
    32701 atctcatgta cttgtggtaa aaaaggctgc cttgagacag ttgcttcagc aacagggatt
    32761 gtcaacttga ctcgtcgcta tgccgatgaa tacgaaggcg atgcagcctt gaaacgcttg
    32821 attgataacg gagaagaagt aactgctaag actgtctttg atctcgcaaa agaaggagac
    32881 gaccttgctt tgattgttta ccgtaacttc tcacgttact tgggaatcgc ttgtgctaac
    32941 attggttcaa tcctaaaccc atcaacaatc gtcatcggtg gtggtgtgtc agctgcggga
    33001 gaattccttc tacaaggtgt tcaaaaagtt tacgatgaaa atagtttccc acaagtacgc
    33061 acatctacta aattagctct tgcaactcta ggaaatgacg ctggagttat cggagcagca
    33121 tcacttgtat tgcaataaga taatgagaga ggaaaatgct aacctagagt tagtaatgtt
    33181 cctcttttat tttttatgct atactagtta ggacaatgaa tgaggtgaaa cgaaatgaca
    33241 aaagcagata cgatttttaa agagaatatt gaacgcattc taaaggaagg tgttttctca
    33301 gagcaggctc gtcctaagta caaggatggg actgttgcta attccaagta tgtaactggt
    33361 gccttttccg agtacgactt atctaaagga gaatttccta tcacaacctt gcgtcccatt
    33421 gcaatcaaat ccgccatcaa agaagttctc tggatttacc aagaccagtc aaatagctta
    33481 gaagtgctta atgacaagta caatgttcac tactggaatg actgggaagt tggagacacg
    33541 ggaaccattg gtgagcgcta tggtgccgtt gttaagaaac acgacattat caataagctt
    33601 ctcaaacagt tggaaaccaa tccttggaac cgccgcaata ttatttcgct ctgggattac
    33661 caagctttcg aagaaacaga tgggctgctc ccgtgcgcct ttcagaccat gtttgatgtt
    33721 cggcgtgttg atggggaaat ctatctggat gcgaccttga cccagcgctc caatgatatg
    33781 ctggtggccc accacatcaa cgctatgcag tatgtggctt tgcagatgat gattgccaaa
    33841 cattttggct ggaaggttgg gaagttcttc tacttcatca acaacctcca tatctatgat
    33901 aatcaatttg aacaagctca ggaattgctc cgtcgggagc cgtcaaactg ccaaccacgc
    33961 ttggttttaa atgttcctga tgggactaat ttctttgata tcaaagcaga agattttgag
    34021 ttggtggatt atgaccctgt taagccacag ttgaagtttg acctagctat ttaaaagaat
    34081 agaaaaaaga agttgagaat aatcccaact tcttttgttt cttaacgtga tacgcggcga
    34141 cgagctgctt ttttacggtt ttcttcgatg aaagctgctt tttgctcttc tggttcgatt
    34201 actttctttt taaatgcgta tactgcacct gcaacggcag cgacagttcc tgcgacacct
    34261 gttacaagac ctttagcgaa tcctttagcc atgagtcttc ctcctttata ttctcaatca
    34321 gccagcctcc tcaagaggtc acatttttct gactgacctt tttgtgttat aataatagta
    34381 acgaaaaaat gggaattttt caaggaaaaa agatgaaaac aaaaataatt gtgattgttg
    34441 gaccgactgc tgttggaaag acggctctag ctattgaagt tgcaaagcgt tttaatggcg
    34501 aagtggttag tggagatagc cagcaagtct atcgaggact tgatattggg acggccaagg
    34561 ctagtccaga agagcaggca gctgttcctc atcatttaat cgatgttaga gagataaccg
    34621 agtcttactc ggcttttgat tttgtttcag aagctaagat gactattgag ggtattcaca
    34681 accgtggcaa gctagccatt atcgccggtg ggactggact ttatatccag agcttgctag
    34741 aaggttacca cctaggtggg gagactcctc atgaggagat tttagcttat cgagctagtt
    34801 tggagccata ttcagatgag gaattagccc atttggtgga tcaagcaggc cttgagattc
    34861 cccagtttaa tcgtcgtcgt gctatgcgtg ccttagaaat tgcccatttt ggtcaggatt
    34921 tggaaaatca agagaccttg tatgaaccgc tgattatctg cttggatgat gaacgtagtc
    34981 aactttatga gcgtatcaac caccgagtgg atttgatgtt tgaggctggg cttttggatg
    35041 aggccaagtg gctgtttgac cattccccta atgtacaggc tgctaaagga attggctata
    35101 aggaactctt tccttatttc cgtggagagc agaccttgga ggaagctagt gagagtctta
    35161 aacaggcgac tcgtcgtttt gccaagcgtc agctgacctg gttccgtaat cgcatgcagg
    35221 tcacctttta tcagattgga gaatctggtg tacaagaccg cattttaagc cagatagagg
    35281 agtttttaga tgattgaaac ggagaaaaaa gaggagcgag tcctgctgat tggtgtggaa
    35341 ttgcagggta tggacagttt tgacctctcc atggaagaat tggctagttt agcgaaaacg
    35401 gcaggggcag tcgttgtaga tagctacaga caaaaacgtg aaaaatatga ttccaagacc
    35461 ttcgtcggct ctggtaagtt ggaagagatt gcgcttatgg tggatgcaga agaaatcact
    35521 actgtcatcg tcaacaatcg tctgacccca aggcagaatg tcaatctaga ggaagttctc
    35581 ggtgttaagg tcattgaccg tatgcagttg attttggata tctttgccat gcgggctcga
    35641 agccatgaag ggaagctcca agtccaccta gcccaactca aatacctttt gcctcgcttg
    35701 gttggtcagg ggattatgct cagccgtcag gcagggggaa ttggttcccg tggtcctggt
    35761 gaaagccagc tggagctgaa ccgtcgtagc gttcgcaatc aaatcacgga tatcgagcgc
    35821 cagctcaagg tggttgagaa aaatcgtgcg actgtcagag aaaaacgttt ggagtctagc
    35881 acttttaaga ttggtttgat tggttatact aatgctggga aatcaactat catgaacatc
    35941 ttgaccagta agacccagta tgaagcagat gagctctttg cgactctgga tgcgacaacc
    36001 aagagtattc atctgggagg caatctccaa gtaactttga cagataccgt tggctttatc
    36061 caagatttgc cgacagagtt ggtgtccagt ttcaagtcaa ccttggaaga aagcaagcat
    36121 gtggaccttc tggttcatgt tatcgatgct agcaatcctt accacgagga gcatgaaaaa
    36181 acggttctct ccatcatgaa agacctggac atggaagata ttcctcactt gacgctttat
    36241 aataaagcgg atttggtgga ggatttcacg cctacccaaa cgccatatac cctcatttct
    36301 gccaagtctg aggacagtcg tgaaaacttg caagcattat tgctagataa gattaaggaa
    36361 atttttgaag catttaccct gcgagtgcct ttttcaaagt cctacaagat tcatgattta
    36421 gagagtgttg caattctgga agaacgtgat tatcaggaag acggcgaagt gattacaggc
    36481 tacatttccg agaaaaataa atggaggtta gaagaatttt atgactgata ttaaaacttt
    36541 ggctctaaag tatggaggtt atacaagtct ggacaaggtc tatctggatc agcttctagc
    36601 tggtaaaaca gatcaggagc agttaacact tattacacct ccgccaagtg ttgttaatgc
    36661 ctactttgca gaactctatc agaaaaagag tcctcaggct gcgacggatt attttgcaga
    36721 actcagtcag gaactgaatc tttacaatac tgagccaagt ttcaccctag aaaataagcc
    36781 ttttattcgg cttaatctgt ctggtaaatc ctttggtttt tgttatgaga gtgacggcct
    36841 tggtcggatt ttctttgaaa atgaggagaa aatctcggat gacttgctct ttgaaattgc
    36901 gcaaattttc ccccatcaat tagtctttga agagtctggt aagatttaca tgaagcctgt
    36961 cgaggacgag gaagttatca gcctagaaag tctcacagct ttgactgatt tggaaagatt
    37021 ggctgatggt cgaaaacgtc tcaaaggcta cagccaagag gatttattgc aagaagctgc
    37081 tgctttttct ggcaaacgct atttccgatc ggaaaaccgc acagccatgt tatatattga
    37141 ttaattagaa agtatcgaat ggatattcaa tttttaggaa cgggggctgg tcagccctct
    37201 aaagcccgca acgtttcaag tctcgccctg aaacttttgg atgagattaa cgaagtttgg
    37261 ctctttgact gtggagaagg tacgcaaaat cgcattctgg aaaccacaat tcgaccacgt
    37321 aaggtcagca aaatctttat tacccatctg catggagacc acatttttgg tttgccaggt
    37381 ttcctttcta gccgtgcctt tcaggccaat gaagagcaga cagatttgga aatctacgga
    37441 cctcaaggaa tcaagtcatt tgtcttaacc agccttcgtg tgtcaggttc tcgtctgccc
    37501 taccgcattc atttccatga gtttgaccaa gattctctgg gtaaaattct tgaaaccgat
    37561 aaattcactg tgtatgcaga ggagctggac cacactattt tctgtgttgg ctatcgtgtc
    37621 atgcaaaagg atctagaagg gacgctggat gctgaaaaac tcaaggctgc tggtgttccg
    37681 ttcggcccgc tttttggtaa aatcaaaaac ggccaggatc ttgttttgga agacggaact
    37741 gaaatcaagg cagcagacta tatctcagcg ccacgtccag gtaagattat cactatttta
    37801 ggagacactc gaaaaacggg tgccagtgtg cgtctggctg tcaatgcaga tgtcctagtt
    37861 catgagtcca cttatggcaa gggtgatgaa aaaattgctc gtaaccatgg tcactcaact
    37921 aatatgcaag ctgcacaagt agcggtagaa gcaggtgcca aacgcctcct actcaaccat
    37981 atcagtgccc gtttcctctc aaaagatatt agcaaactca agaaagacgc tgccacaatt
    38041 tttgaaaatg tccatgtggt caaagacttg gaagaagtgg aaatctagca gtcacagaaa
    38101 ggataagtat gcctactatt ctcattaccg gagctagcgg tggtctagct caagaaatgg
    38161 tcaaactcct gcccaatgac caactcatct tgcttggtag aaataaggaa aaattagccc
    38221 aactctacgg aaattattcc catgcagaat tgattgaaat tgatattacc gacgattcag
    38281 ccctagaagc tctggtaact gatctttatc tccgctatgg caagattgat gtcttgatta
    38341 acaacgctgg ttacgggatt tttgagggat ttgaccagat tgctgataaa gatattcacc
    38401 agatgtttga ggtcaatacc tttgccctga tgaatctgtc tcgtcacctt gcggctcgta
    38461 tgaaggaaag cagcaaaggg catatcatca acatcgtcag catggcaggt ctaatagcta
    38521 ctggcaagtc tagtctttac tcagcgacca agtttgcggc tattggtttt tcaaatgctc
    38581 tgcgactcga acttatgccc tatggagtct atgtgacaac agtcaatcca ggaccaatcc
    38641 gtacaggatt tttcgaccaa gccgacccag atggaactta tcttaaatcg gttgatcgct
    38701 tcctgctaga accagatgca gtggctaaaa agattgtcaa gattatagga aaaaacaaac
    38761 gagaactcaa tctcccgatt ttgttgaacc tagcccataa gttttatact ctctttccca
    38821 agctagctga taagttggca ggggaaactt ttaattataa gtaaaaagaa ccaatgtgca
    38881 ggttgttgct agcctacata ttggttctta tcatttctca actatcaaac tgaacttctt
    38941 ctaggagata gtctataaag cgttcgccca tcttagatag gctggttttc tcatgctgga
    39001 tatagaccag ctcgatcggg tcatcaatat ccagtgggat agaaacgata ttgtctccgt
    39061 ttaggttgct gttcaaaatc cctgtcgcaa tggtataacc atccaaacca atcaagagat
    39121 taaagagggt ggcacggtca ctgaccacaa tggatttctt gtggtgttct tgagaaagaa
    39181 tctcttctga aaagtagaag gagttgtgcg tcccttggtc atagctgagg taagggaaat
    39241 tctccaaatc agacagtttc accttgtctt tctttgccag agggttggtc ttgctgacaa
    39301 agatatgcgg ttgcgctgtg aagagatggt gggctagcag gtgattgtca tccagcatct
    39361 tggttaaaac atcacggttg taactgttta agaagaggac cccgacctca ctgcggaagt
    39421 tcttgacgtc gtcgataatc tcccaagtcc gagtttcacg aaggaagagt tcgtatttct
    39481 ccatatcgct tttcttgagc aaagagacaa aggcattgac cacaaaggca tagtgttgag
    39541 acgaaacgct aaagagttcg cggtgggcga caggattttt atagcgttcc tccagaagct
    39601 gggtctgctc gacaacctga cgggcataag agagaaactc catgccatca cgggtcaagg
    39661 tgattccctt gggattgcgg ataaagatct caatgcccat ttcattttcc aaatctcgca
    39721 ctgcattgga gagacttggc tgagtgataa agagttgctt ggctgcctca ttcatggagc
    39781 cagtttcgac gattttgata atataatgta attgttgaat tctcatgttt ttattgtacc
    39841 atacttgcct tagaatcgct aaagaagata ggaaaataag gagcattgtg tgagtctctt
    39901 cttgaaaaaa acacaaaatt ttgctagaat gagtcttatg aaaacattct atgatgtgca
    39961 gcaattcctc aaacgatttg gtattattgt ttacatggga aaacgcttat atgatattga
    40021 actgatgaag ttggaactat ctcggattta cgatgcaggg ttgatggata aattagacta
    40081 tctagaggca gaagcagttc ttcgcagaga gcacaaggta gaattggatt atattgagaa
    40141 aaatggagaa aagaactaat ggttacttgg attttgtggg cacttatact agcaatgttg
    40201 gcgtggatgg gctttaacta tcttcgtatt cgccgtgcgg ctaaaattgt ggacaatgag
    40261 gagtttgaag ccttgattcg tacgggtcaa ttgattgatt tgcgcgaccc agcagaattc
    40321 cacagaaaac atatccttgg tgcacgcaat attccttcaa gtcagttgaa aactagtctt
    40381 gcagcccttc gtaaagataa acctgtcctt ctctacgaaa accaacgtgc gcaacgagtt
    40441 acaaatgcag ctctttactt gaaaaaacaa ggtttttctg agatttatat cctttcttat
    40501 ggcttggatt cttggaaagg gaaagtgaag actagctaac cttatttcca aattagtcga
    40561 atgataggat gaagctattg ttttatagta taattaattt gtataaaatt taaggagttc
    40621 ttagaacata aaagaaaaga agaaaaatcc actttttgtg ggtatcttgt cgattattct
    40681 tggtttgtta tttccaattg taggcctgat tttgggtatc ataggattgg tcttggctat
    40741 ttcataccaa aaagagtcac aattagacta taaaatagaa aagattctta atatcttagg
    40801 aattgtgatt tctgtagtta actggattgt agcaatcgcc ttaatttttc ggtaaataaa
    40861 agaaaaaagc tttagtttta aagctttttt tgttttatcg actcatctct aaatagtttt
    40921 taatgatttg taactctttt tggttcaaag ggcggtagtt accttctgct aaatctgggt
    40981 tcaatgtgaa gtccccaaat tggattcttt tgaggctagt caccttaaca ccaaccgaga
    41041 ggaacatttt cttgatttga tgaaattttc cttctgaaat ggtgatagag gcttgactga
    41101 gggagggact tgcagataga atctctagtt ttgcgggttt acagacagtg tcatctaaaa
    41161 agacaattcc tttttgaaag gtttggatat ggtcaggtgt tagaagtcca ttaacctcaa
    41221 cttggtaagt cttatcgaca tgatattggg gatggaggag ctgaaagccc aagggaccgt
    41281 tatcggtcaa gaggaggagt cccgttgtat ctcggtccag tcggccaacg gcatagagct
    41341 tgtcagactg gatgttagat ggaagcaggt ccatgacggt cggaagttcc ttgtctttgt
    41401 tggctgtaac ggcaccagca ggtttatgaa gcataagata ggtgtgttca tagccttgaa
    41461 tgattcgatc ctgaaaaagg agttcttgta gtcctgtatc gatattttga gctagggagc
    41521 gggctgggca accatcgact agaatttccc ctctgaggag tgcttgcttc atggcctttc
    41581 ggctgatttt ttcttgagct aataaattat ctaaacgcat actgtgccta tcttatcata
    41641 agtcgctcgc tttgaaaagg cttgacgtga aaagaaaagc agagtgaaaa catttacact
    41701 tgcttgagtt atgttattta tttgaaatta tggtataatc gttcagttag aaaataaatt
    41761 ttgaatatta tagaggaaat catgacaaaa ttaagagaag atatccgtaa cattgcgatt
    41821 atcgcccacg ttgaccacgg taaaacaacc ctggttgacg aattattgaa acaatcagaa
    41881 acgcttgatg cacggactga attggcagag cgtgctatgg actcaaacga tatcgaaaaa
    41941 gagcgtggaa ttaccatcct tgctaaaaat actgccgttg cttacaacgg aactcgtatc
    42001 aacattatgg acacaccagg acacgcggac tttggtggag aagttgagcg tatcatgaaa
    42061 atggttgacg gtgttgtctt ggtcgtagat gcctatgaag gaaccatgcc acaaactcgt
    42121 ttcgtattga aaaaagcctt ggaacaagac cttgtcccaa tcgtggttgt taacaaaatc
    42181 gataagccat cagctcgtcc agcagaagta gtggatgaag tcttggaact tttcatcgag
    42241 cttggtgcag atgacgacca gcttgatttc ccagtggttt atgcttcagc gatcaacgga
    42301 acttcttcat tgtcagatga tccagctgac caagaagcga ctatggcacc aatctttgac
    42361 acgattatcg accatatccc agctccagta gataactcag atgagccttt gcagttccaa
    42421 gtgtcacttt tggactacaa tgacttcgtt ggacgtatcg gtatcggtcg tgtcttccgt
    42481 ggtacagtta aggttgggga ccaagttacc ctttctaaac ttgacggtac aactaaaaac
    42541 ttccgtgtta caaaactctt cggtttcttt ggtttggaac gtcgtgaaat ccaagaagcc
    42601 aaagcgggtg acttgattgc cgtttcaggt atggaagaca tctttgtcgg tgaaaccatc
    42661 actccgacag atgcagtaga agctcttcca atcctacaca tcgatgagcc aactcttcaa
    42721 atgactttct tggtcaacaa ctcaccattt gctggtaaag aaggtaaatg ggtaacttct
    42781 cgtaaggtgg aagaacgctt gcaggcagaa ttgcaaacag acgtttccct tcgtgttgac
    42841 ccaactgatt caccagataa atggactgtt tcaggacgtg gagaattgca cttgtcaatc
    42901 cttatcgaaa caatgcgtcg tgagggctat gaacttcaag tatctcgtcc agaagttatc
    42961 gtaaaagaaa tcgacggtat taaatgtgaa ccatttgaac gtgtacaaat cgacactcca
    43021 gaagaatacc aagggtctgt tatccaaagc ctttctgaac gtaaaggtga aatgttggat
    43081 atgatttcaa ctggtaatgg tcaaactcgt ttggtcttcc ttgttccagc gcgtggtttg
    43141 attggatact caactgagtt cttatcaatg actcgtggtt acggtatcat gaaccatacc
    43201 ttcgaccaat acttgccatt gattccaggg gaaattggtg gacgtcaccg tggtgccctt
    43261 gtttctatcg atgctggtaa ggctacaact tactcaatca tgtctatcga agaacgtggt
    43321 acgatctttg tcaacccagg tactgaggtt tatgaaggaa tgatcatcgg tgaaaactct
    43381 cgtgaaaatg acttgacagt taacatcacg aaggcaaaac aaatgaccaa cgttcgttca
    43441 gctactaagg accaaacagc tgttatcaag acgcctcgta tcttgacact tgaagagtct
    43501 cttgagttct tgaacgacga tgagtacatg gaagtaacgc ctgagtctat ccgtttgcgt
    43561 aaacaaatcc ttaacaaagc agagcgtgag aaagctaaca agaagaaaaa atcagctgaa
    43621 taagagctag aaagagagaa agatggtcta tttagtccta ggaattttac tgctcctact
    43681 ctatgtattt gcgacaccag aaagcattaa agggactgtc aatatcgtcg ctatggtatg
    43741 tattttagtg gcactcttga ttttattggt tctatctttt ctgaaaattt ttcaattacc
    43801 aacagaaata ttcctagcaa tagccatgtt gatcctagct tactttagtg ttagagacat
    43861 cacactcatg ccagtcaaaa aaagtaaaag aagataaaaa agagagtttc ggctctcttt
    43921 ttgctaggaa ttttacaata gtttgaattg ctttttagtt aacaccatct cttttacttg
    43981 tatagtatga atgggaaaaa cttgaataaa attcgttaat ggcatctatt ttatatttgg
    44041 ttagtactgg ttgcatatta ttttgaaata aaactactat ataatattat tcacagaggt
    44101 aatatagtaa aatatctaaa aaattcgatt ttttccttga caacaaataa taaaaaagtt
    44161 attcttatca cataagaaag cggtttcaat aaaagagaga ggaggattta ttttgttgca
    44221 ttgttgctca aggtgtgctt tttttgaaag aaaaaataaa atgaaaacga aaaaacatag
    44281 attacttgct ctagctctta tttcaagttt tacattattg ggagctgcat cagctgctgt
    44341 ataatatcca gatggaggag tatggacata tggagaaggt tcaggaggtg gttaggcttt
    44401 ttcaaattac tatcatggta aaaaatatca ttattcatct atcgtgagca gatgggatgg
    44461 tcattctgat aaaggagaag ctcctgctgg aaaaacctcc tatgcatgga tttggactaa
    44521 atggggagaa caagtagcat tttactgtga ttatgactaa tcgttcattg atttaaatcg
    44581 ttccccttgt aagaaattac agggggaatt ttgtttacaa ggagtctact atgaagaaac
    44641 tattcatatt attatcaact ttttttctca gcttcttcct tgcttggatt attgtcttac
    44701 gtgcgccaca atatttatat gcaagctatg attccgtttc tttacttcgt gtcaaaaaag
    44761 atactcagga accgacgcgt gaggtatttg aacaggaatt ggagaatttt gcaaactcag
    44821 aacagagttt aatagctaga agaattgtag agccgagtaa ggatgggacg actcacttta
    44881 cttatgcaac ttatggtcag ggaactttac caaaagaatt ccaagaagct agccaagaaa
    44941 gtcgtgaacg tagtgatccg ctaaatagtt atctcctttt gtcaggctcc ttgacgaaag
    45001 aaaagcttgc cgataaatta ggagatttgg gttataaagc aagtgctgac cgaaagatac
    45061 cgccctattt tcttgctttt cgaatattac taaatcccct tattttaatt agtttagcaa
    45121 tatttggctt atctttcttt gctttagtga ttatcactcg gattaaggaa atgagagcag
    45181 caggtataaa actcttttct ggtcagactc tcttatccat catggggcat tctttatcta
    45241 ctgatatcaa atggctcctt ctatcagccc tcctttcctt cctaggtggg ggagtcgttc
    45301 tttttagtca aggtttgttt tatcctatct tgttagccac ctatggtttt gggattagtt
    45361 tctatctgtt gtttttattg gcgatttcaa ttttactaat gctcctttac ctaatgagtt
    45421 tgagttacaa agcattagtt cccgttatta agggaagatt accccttaaa cgcttgatgg
    45481 ctttaaccct attgtgtcag ttagtagctg tttttacagt aggctacgct gttaagacag
    45541 gtttgacgtc ttaccaacga ttgaaagaac ttgaaatttc aaaacaagca tggcaggaca
    45601 gagcagacta ttaccagatt tcctttggct taggtgatag aggaaaagat acagaaaatc
    45661 agagcaagtg gtatgccttt gccaaggaag caatcgaaga agaacaagct ctttatgtaa
    45721 aggataatct gctccatttt gccaatccac aaggaaaaaa tgaacaggga gagacactgg
    45781 atacctatag tccagatgct aatacgctct atgttagtcc cagttatttg gacaaggaaa
    45841 aggtcgtggt agatgctgag accaaacaga agttagccca tctccaaaaa ggtgagttta
    45901 tcctcttgct cccagaacat ttgcgctctc gagaagcaga acttaagaaa gtttttgaag
    45961 aaagattgag ttattatgga aaatctggtg aggaggcaag tgctcctttg gattatgaga
    46021 tgaaagcgca cgttagttat ctttcaatgg gagaaaagcg gtttgtttat aataacggtg
    46081 agaatcccgt atctactcag tatttgactg atccgatttt agtggtattc acgccgactt
    46141 ctacaggtga tagttttata tccttatcta gttggtctat caatgctgga aaacaactct
    46201 ttatcaaagg atatgagagt gggctagaac tcttgaagaa agctggaatt tatgagcaag
    46261 tatcctatct taaagaagga agaagtgttt atctaactcg ttataatgaa gttcaaactg
    46321 aaacagcaac tttaatctta ggagctattg ttggaatagc cagttccttg ttactctttt
    46381 attctgtcaa tcttctatat ttcgagcaat tccgccgaga tatcttgatt aaacgaattt
    46441 caggtttacg attttttgaa acacatgctc agtatatggt tagccaattt gctagttttg
    46501 tatttggtgc tagtctcttt attttaagca gtcgagactt ggtgattggc ttgctcactt
    46561 tattggtctt tctagctagt gcagttttga cgctttaccg tcaagcgcag aaagaatctc
    46621 gtgtttctat gacaattatg aaaggaaaat aggatgattg aactaaagaa tatatctaaa
    46681 aaatttggaa gccgtcagct attttcagat atgaatcttc attttgaagg tgggaaaatt
    46741 tatgccttaa tcggtacaag tggctgtggt aagacaacac tcttgaatat gattggacga
    46801 ttggagccat atgacaaagg gcaaatcatc tatgatggca cttctcttaa ggacatcaag
    46861 ccttctgttt tctttagaga ttacttagga tacttatttc aagattttgg cttaattgaa
    46921 agccaaaccg tcaaagagaa tctcaatctg ggtttagttg gtaaaaagtt gaaggaaaaa
    46981 gagaaaatct ctttgatgaa acaagctcta aaccgtgtaa acctctctta tttggatttg
    47041 aagcaaccta tatttgagtt atcaggagga gaagcacaac gtgttgcact agcgaagata
    47101 attttaaagg atccaccttt gatcctcgca gatgaaccaa ccgcttcctt agaccccaaa
    47161 aactctgagg aattactttc catcctagaa tctttaaaaa atccgaatcg gaccattatt
    47221 attgcgaccc ataatcctct gatttgggag caagtggatc aggtcattcg agttaccgat
    47281 ttatcacata gatgatatgg taagattcag ttagaagaaa gagtcacaaa cacactttgt
    47341 ggctttttta tttccataaa aatggtaaaa tagtaggagt agaaatggag ttcgagacat
    47401 gaaagtaata gatcaattta aaaataagaa agtccttgtt ttaggtttgg ccaagtctgg
    47461 tgaatctgca gctcgtttgt tggacaagct aggtgccatt gtgacagtaa atgatgggaa
    47521 acctttcgag gacaatccag ctgcccaaag tttgctggaa gaagggatca aggtcattac
    47581 aggtggccat cctttggaac tcttggatga agagtttgcc cttatggtga aaaatccagg
    47641 tatcccctac aacaatccca tgattgaaaa ggctttggcc aagggaattc cagtcttgac
    47701 tgaggtggaa ttggcttatt tgatttcaga agcaccgatt attggtatca caggatcgaa
    47761 cggtaagaca accacaacga ctatgattgg ggaagttttg actgctgctg gccaacatgg
    47821 tcttttatca gggaatatcg gctatccagc tagtcaggtt gctcaaatag catcagataa
    47881 ggacacgctt gttatggaac tttcttcttt ccaactcatg ggtgttcaag aattccatcc
    47941 agagattgcg gttattacca acctcatgcc aactcatatc gactaccatg ggtcattttc
    48001 ggaatatgta gcagccaagt ggaatatcca gaacaagatg acagcagctg atttccttgt
    48061 cttgaacttt aatcaagact tggcaaaaga cttgacttcc aagacagaag ccactgttgt
    48121 accattttca acacttgaaa aggttgatgg agcttatctg gaagatggtc aactctactt
    48181 ccgtggtgaa gtagtcatgg cagcgaatga aatcggtgtt ccaggtagcc acaatgtgga
    48241 aaatgccctt gcgactattg ctgtagccaa gcttcgtgat gtggacaatc aaaccatcaa
    48301 ggaaactctt tcagccttcg gtggtgtcaa acaccgtctc cagtttgtgg atgacatcaa
    48361 gggtgttaaa ttctataacg acagtaaatc aactaatatc ttggctactc aaaaagcctt
    48421 gtcaggattt gacaacagca aggtcgtctt gattgcaggt ggtttggacc gtggcaatga
    48481 gtttgacgaa ttggtgccag acattactgg actcaagaag atggtcatcc tgggtcaatc
    48541 tgcagaacgt gtcaaacggg cagcagacaa ggctggtgtc gcttatgtgg aggcgacaga
    48601 tattgcagat gcgacccgca aggcctatga gcttgcgact caaggagatg tggttcttct
    48661 tagtcctgcc aatgctagct gggatatgta tgctaacttt gaagtacgtg gcgacctctt
    48721 tatcgacaca gtagcggagt taaaagaata atatgaaaaa aattgtcttt acaggtgggg
    48781 ggacggttgg acacgttacc ctcaatcttt tgttaatgcc caagttcatc gaagatggtt
    48841 gggaagtcca ctatatcggg gacaagtgtg gtatcgaaca ccaagaaatc cttaagtcag
    48901 gtttggatgt caccttccat tctattgcga ctggaaaatt gcgtcgctat ttctcttggc
    48961 aaaatatgct ggacgtcttc aaagttggtt ggggaattgt ccaatcgctc tttatcatgt
    49021 tgcgactgcg tccacagacc cttttttcaa aggggggctt tgtctcagta ccgcctgtta
    49081 tcgctgcgcg tgtgtcagga gtgcctgtct ttattcacga atctgacctg tctatgggct
    49141 tggccaataa aatcgcctat aaatttgcga ctaagatgta ttcaaccttt gaacaagctt
    49201 cgagtttggc taaggttgag catgtgggag cggtgaccaa ggtttcggac aaaaacactc
    49261 cagaaccaga tgaattggtg gatattcaaa cccactttaa tcccaaattg ccaactgtat
    49321 tgtttgttgg cggttctgca ggcgctcgtg tctttaacca attggtgaca gaccataaga
    49381 aagaactaac agagcgctac aatattatca atctaactgg agattctagt ctgaatgagt
    49441 tgaggcaaaa tctttttcgt gttgactatg tgaccgatct ctatcaaccc ttgatggaat
    49501 tggctgatat tgttgtgaca cgaggtggtg ccaatacgat ttttgagctc ttggcgatag
    49561 caaaattgca tgtcattgtg ccgcttggtc gtgaagctag tcgtggtgac cagattgaaa
    49621 atgcagctta ctttgttaag aaaggttatg cagaagacct tcaagaaagc gatttgacct
    49681 tggatagttt ggaagagaag ctttctcact tactaagtca caaggaagat taccaagcta
    49741 agatgaaagc ttctaaggaa ttgaaatctc tagcagattt ttatcaattg ttgaaaaaag
    49801 atttatcata aggaaagtaa atgtcaaaag ataagaaaaa tgaggacaaa gaaaccctcg
    49861 aagaattgaa agagttatca gaatggcaga aacgaaacca agaatatcta aaaaagaagg
    49921 ctgaagaaga ggcggctcta gctgaggaga aggaaaagga aagacaagct cgaatgggag
    49981 aagaatctga gaagtcagag gataaacagg accaggagag tgaaacagac caggaagatt
    50041 cagaatcagc taaggaagag tctgaagaaa aagtagcatc ctcagaggct gacaaagaga
    50101 aagaagagaa agaagaacca gagtctaaag agaaggagga acaggataaa aagcttgcta
    50161 aaaaggctac aaaggaaaaa ccagccaaag caaagattcc tggtatccat atcttgcgag
    50221 ccttcacgat tttatttcca agtctgcttc tattgtttgt ctctgcctac ttgctcagtc
    50281 cttatgcgac catgaaagat attcgtgttg agggaacggt gcaaactaca gctgatgata
    50341 ttcgacaggc ttcaggcatt caggattcgg attatacgat taaccttctg ctagacaagg
    50401 caaaatatga aaagcagatt aagtctaact attgggttga atcagctcaa cttgtctatc
    50461 aatttccaac taagttcact attaaggtca aggaatatga tattgtggcc tactatattt
    50521 ctggtgaaaa tcattatcct attctttcca gtggtcagct tgagactagt tctgtgagtc
    50581 tgaacagttt accagaaact tatttatcag ttctctttaa tgatagtgaa caaatcaaag
    50641 tttttgtctc agaacttgct caaattagcc cagaactcaa ggcggctatc caaaaggtgg
    50701 aattagcccc aagcaaggtg acatccgatt taattcgatt gaccatgaat gattcggacg
    50761 aagtcttggt tcctctatct gaaatgagta agaaactgcc atattacagt aagattaagc
    50821 ctcaattgtc agaaccgagt gtggtcgaca tggaagctgg aatctacagc tacactgtgg
    50881 cggataaatt aattatggaa gctgaggaaa aagccaaaca agaggccaag gaagctgaga
    50941 aaaaacaaga agaagaacag aaaaaacaag aggaagagag caatcgaaac caaacgaccc
    51001 agcgttcatc gcgtcgctag gtttaccttt tctcttatag ttctttagtg accatgtttt
    51061 tacgtttaat atttgacatc tgtttttctt tatgttacat ctgcaatgta atcgattaca
    51121 aaataatttt tgatgaagaa ggtaacacat atgtcagatg aagtattctt atttgaagaa
    51181 attgaagaaa tcgtagcccc tacagatggt gaatttttgg gggaagtttt acttggaact
    51241 ggggtagttc tcttaattgg agtagcctgt tgttaaaaag atagggagtg ataatcatgc
    51301 aagataactt tttatttgag gaaattgaag aaatttcagt accagttaat gatttttcag
    51361 ctggacttgc aacaggtatc ggatttggtt tagcaatcct tgctcttgct ggttgttgaa
    51421 gtttgttcat ttactaacat caagcttttt caatttcatt ttagacagtc atttaaattt
    51481 tccgtattag tcttgcagca agagattaat agaattagtc attattttat tgattgcgga
    51541 ctgagggact agagtatgtt ttacttaacc cctcttttat ttattaaagg ttaggtttgt
    51601 tatgagaatt gttgataaga ttaagatatt acctactcct tatgagggac actatcattt
    51661 atatatacca tccagtaaga aacatgtatt agttgggaaa caggaaaaaa atggttagag
    51721 caactaatag gtcaagaatt taccatatcg gacttattag tgttagtagg gaagaaatat
    51781 ttttaaaata tcttgggact ttaatataac attatctgaa aaattaaact ataaaagatt
    51841 taataagaat tttgaaaaaa tcctatcttg ttgtcattat atttgcaacg atacatgaaa
    51901 ttagtcatgc aataattgct aataattatc aaattgagat cccaaattgt ggaataatgc
    51961 tacttttgtt taatttggct ttttttgtag atttaagtgg ggtgcaattc ctaaaaaata
    52021 aaaaacaatt tttgaaaatt atgttagcag gaattgcttc aaattcgatt ttatcactta
    52081 caggtttact tgttttattg ttcacatcgt ataaattgct tggactctta ttttttatca
    52141 ttaacttagg tatgattttt attaattcaa ttcctttttt tcagtatgat agtggtatta
    52201 ttttaagata cttgaattct aacaataata acttgaattt tcaatatata gttcaacttt
    52261 taatagcatt tgttattatt tattttcctt tgagtcaact attacagttt ttgacaccca
    52321 atattattgt tcgtagtata ggaggggtgg ttgtttctat actgctttct atattatata
    52381 tgataggaag gacgaaatat gttctacgta aatagttatg tttttgctta taaaaaagaa
    52441 ggtataatgt atttacgtgg tcggagtatg cgggaaatag ctatagaacc tcaaatttcg
    52501 caagaattta tcaacgatct atttaatagt tgtaaggaac tattagagat agaagaagta
    52561 ttaggcagta aactaacatt tgaactataa atgaacaaat tttaatttcg gatgagatag
    52621 atattgatag tagatattct agaactaaag gttactattc gttattttat aatgaagagt
    52681 ataataaaat acagaataaa acagtattag tattaggagc aggagtctta ggatgttata
    52741 tatctctaag tctaagtatg tatggagtga ggaaacttat tgtcgctgat tacgatataa
    52801 tagaaccatc aaatttaaat aggcaaattc tttatacaga gtcggatgtt ggtaaggaga
    52861 agattaatgt tctttctgaa aaaatacaca agtataattc agatgttcag gtagtaccta
    52921 tttctattaa agtttcttca gtagaagaat tagaaaaaat tgttgcggaa tatgggagta
    52981 tagattttat cgttaaagca attgatacgc ccattgatat tataaaaatt gtcaatcaat
    53041 ttgctgtatc gcataagata tcctacatat caggagggtt taatggatgc tatcttatta
    53101 ttgataatat atatatccct accatcggtt cttgctttgg ttgtcggaat ataaacaaag
    53161 atataaataa gtacacttta tctgataaga caaagtggcc gactacacca gagatgcctg
    53221 ctattttggg agggataatg actaatttaa taattaaaat atttctggga tgttataatg
    53281 aaatcctaat agataacgct tacgtttata atatgagaaa tcatgctcta agtcaagaaa
    53341 aatatgttct ggaaaacgga gaatgtccaa tttgtaaaaa aataataaag tgaaagataa
    53401 caatattaga gcgaaaacat ttattcgttc agtttgtttt tgcttattat caggaggagt
    53461 agctttttta tctgctattg ggcagttcac tgttatagaa acacaattaa tagtattgtt
    53521 cttgggtatt atttttgcta tatattatgc ttactacaat aaaaatattc aaacatcatt
    53581 ggaaaatata gtatggcttt tttcatcgtt tgagatttta tttttgcttg ttaattttag
    53641 aacatttatt cagttaccag tggatatttt tattggtatg ataatatttt taatgctgtg
    53701 gatatttatt atgttaggta tagtgtgtct tagttattat ataactttat tatttagcaa
    53761 ggaggcttag tatgtttaaa aaaata
//
LOCUS       DAVKNL010000006        50894 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVKNL010000006 DAVKNL010000000
VERSION     DAVKNL010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 50894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50894
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.006"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            95..1303
                     /locus_tag="VW411_000318"
     CDS             95..1303
                     /locus_tag="VW411_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9793118.1"
                     /translation="MNYIKRPHYLDFLRKHRDRPIIKVVSGVRRAGKSVLFQLYKEEL
                     LATGVDEDQIIFINFEDLSYYDLRHFQTLFAYIKDQLVSKKTYYIFLDEIQYVEKFEL
                     VADSLFILANVDLYLTGSNAYFMSSQLATNLTGRYVEIEVLPLSFEEYLSGQSLTENL
                     NTTEIFNNYLFSAFPYLLQTSSYDEKIDYLRGIYNSILLNDIVTRLGKPNPTIIERIV
                     RTLLSSTGSLISTNKIRNTLVSQNVSISHNTLENYLTTLTDSLLFYSVPRFDVKGRAL
                     LQRLEKYYPVDLGLRHLLLPDQKEDIRHILENMVYLELRRRYSQVYVGNLDKYEVDFV
                     VVTDLGHYAYYQVSETTLAPETLERELRPLEAIKDQFPKYLLTMDTIQPTANYNGIEK
                     KSIIDWLLEK"
     gene            1679..2083
                     /locus_tag="VW411_000319"
     CDS             1679..2083
                     /locus_tag="VW411_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9793119.1"
                     /translation="MLFFSISNDLLQLLTSWEKQQRYELAKFGILSNPEQFVFTYIDT
                     KGNINKPLHADYLNNKMKSIRKRHKELTHATPHKLRHTGATLAKKAGMSLEAISEALT
                     HSDTVTTKTYVNTSNIVPLSAGQVAYQHLKNK"
     gene            complement(2346..2738)
                     /gene="rpsI"
                     /locus_tag="VW411_000320"
     CDS             complement(2346..2738)
                     /gene="rpsI"
                     /locus_tag="VW411_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU9793120.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVIGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(2757..3203)
                     /gene="rplM"
                     /locus_tag="VW411_000321"
     CDS             complement(2757..3203)
                     /gene="rplM"
                     /locus_tag="VW411_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEU9793121.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            3559..3876
                     /locus_tag="VW411_000322"
     CDS             3559..3876
                     /locus_tag="VW411_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEU9793122.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            complement(4026..4838)
                     /gene="folK"
                     /locus_tag="VW411_000323"
     CDS             complement(4026..4838)
                     /gene="folK"
                     /locus_tag="VW411_000323"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEU9793123.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFEFYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIHILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(4881..5435)
                     /gene="folE"
                     /locus_tag="VW411_000324"
     CDS             complement(4881..5435)
                     /gene="folE"
                     /locus_tag="VW411_000324"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEU9793124.1"
                     /translation="MDTQKIEAAVKMIIEAVGEDANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            complement(5416..6738)
                     /locus_tag="VW411_000325"
     CDS             complement(5416..6738)
                     /locus_tag="VW411_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU9793125.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEVIAEQKAGIIKQGMPLVTGRIAPEALAVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALEDMLDLLGAMPVTELTLTHFADSRATDENVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            complement(6740..7714)
                     /gene="folP"
                     /locus_tag="VW411_000326"
     CDS             complement(6740..7714)
                     /gene="folP"
                     /locus_tag="VW411_000326"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU9793126.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGADLVNDITGLMGDEKMAYVVAEARAKVVIMFNPVMARPQHPSSLI
                     FPHFGFGQTFTEKELADFETLPIEDLMVAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEENGFEVNPETELGFRNRDTAS
                     AHVTSIAVRQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            complement(7809..8516)
                     /locus_tag="VW411_000327"
     CDS             complement(7809..8516)
                     /locus_tag="VW411_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9793127.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVAGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            complement(8569..9987)
                     /locus_tag="VW411_000328"
     CDS             complement(8569..9987)
                     /locus_tag="VW411_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEU9793128.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHVMIWILDALFILNYISMAL"
     gene            10177..10989
                     /locus_tag="VW411_000329"
     CDS             10177..10989
                     /locus_tag="VW411_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9793129.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKRTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYSLAKEMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            11329..12348
                     /gene="adhP"
                     /locus_tag="VW411_000330"
     CDS             11329..12348
                     /gene="adhP"
                     /locus_tag="VW411_000330"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEU9793130.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
     gene            12581..13570
                     /gene="manX"
                     /locus_tag="VW411_000331"
     CDS             12581..13570
                     /gene="manX"
                     /locus_tag="VW411_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEU9793131.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            13598..14401
                     /locus_tag="VW411_000332"
     CDS             13598..14401
                     /locus_tag="VW411_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEU9793132.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            14425..15336
                     /locus_tag="VW411_000333"
     CDS             14425..15336
                     /locus_tag="VW411_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9793133.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(15392..16726)
                     /gene="pepC"
                     /locus_tag="VW411_000334"
     CDS             complement(15392..16726)
                     /gene="pepC"
                     /locus_tag="VW411_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEU9793134.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
     gene            complement(16944..17666)
                     /locus_tag="VW411_000335"
     CDS             complement(16944..17666)
                     /locus_tag="VW411_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU9793135.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            complement(17839..17913)
                     /locus_tag="VW411_000336"
                     /pseudo
     CDS             complement(17839..17913)
                     /locus_tag="VW411_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(17934..18164)
                     /locus_tag="VW411_000337"
     CDS             complement(17934..18164)
                     /locus_tag="VW411_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU9793136.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            complement(18174..19415)
                     /locus_tag="VW411_000338"
     CDS             complement(18174..19415)
                     /locus_tag="VW411_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEU9793137.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGVLNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            complement(19437..19589)
                     /locus_tag="VW411_000339"
     CDS             complement(19437..19589)
                     /locus_tag="VW411_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793138.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            complement(19610..19888)
                     /locus_tag="VW411_000340"
     CDS             complement(19610..19888)
                     /locus_tag="VW411_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEU9793139.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARN
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            complement(19881..20144)
                     /locus_tag="VW411_000341"
     CDS             complement(19881..20144)
                     /locus_tag="VW411_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEU9793140.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            complement(20246..24637)
                     /locus_tag="VW411_000342"
     CDS             complement(20246..24637)
                     /locus_tag="VW411_000342"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEU9793141.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPAFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFESVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYCSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            complement(24741..26822)
                     /gene="fusA"
                     /locus_tag="VW411_000343"
     CDS             complement(24741..26822)
                     /gene="fusA"
                     /locus_tag="VW411_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU9793142.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEEIRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            complement(27247..27717)
                     /gene="rpsG"
                     /locus_tag="VW411_000344"
     CDS             complement(27247..27717)
                     /gene="rpsG"
                     /locus_tag="VW411_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEU9793143.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            complement(27737..28150)
                     /gene="rpsL"
                     /locus_tag="VW411_000345"
     CDS             complement(27737..28150)
                     /gene="rpsL"
                     /locus_tag="VW411_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEU9793144.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            28567..32409
                     /gene="spuA"
                     /locus_tag="VW411_000346"
     CDS             28567..32409
                     /gene="spuA"
                     /locus_tag="VW411_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEU9793145.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPGNANASLEKTEEKPAASPA
                     DPAPQTGQDRSSEPTTSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDD
                     VEKPSENWPNGALSFKDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVE
                     KLVPKMNEAWLDQDYKVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQW
                     PDGTDFTATGKYGRYIDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQ
                     IFLKDDDESIYTNPYYVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHL
                     GNKVTITDVAIDEAGKKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGK
                     LGADLKEEGKQVDLTLWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDST
                     NKLGITDFTGYYYQYQIERQGKTVLALDPYAKSLAAWNSDDSKIDDAHKVAKAAFVDP
                     AKLGPQDLTYGKIHNFKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDY
                     LKDLGVTHIQLLPVLSYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSD
                     PKNPEKRIAEFKNLINEIHKRGMGAILDVVYNHTAKVDLFEDLEPNYYHFMDADGTPR
                     TSFGGGRLGTTHHMTKRLLIDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARAL
                     NPNLIMLGEGWRTYAGDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPA
                     FITGGKRDVNTIFKNLIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKA
                     ENYAEIHRRLRLGNLMVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHL
                     LRDKDGNPFDYPYFIHDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQS
                     TDAFRLKSLQDIKDRVHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKARE
                     FNLGTAFAHLRNAEVLADENQAGPVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTS
                     HESTAEEKPDSTPSKPEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENKPSQATAD
                     SQAEQPAQEAQASSVKEAVRNESVENSSKENIPATPDKQAELPNTGIKNENKLLFAGI
                     SLLALLGLGFLLKNKKEN"
     gene            complement(32509..33558)
                     /locus_tag="VW411_000347"
     CDS             complement(32509..33558)
                     /locus_tag="VW411_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU9793146.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRSEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(33677..35485)
                     /gene="glmS"
                     /locus_tag="VW411_000348"
     CDS             complement(33677..35485)
                     /gene="glmS"
                     /locus_tag="VW411_000348"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU9793147.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEEGLSVLEAFKKALHIIRGSYAFAL
                     VDSQDPEVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDEAGQ
                     VVVDPDIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEVKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(35678..37057)
                     /gene="bglA"
                     /locus_tag="VW411_000349"
     CDS             complement(35678..37057)
                     /gene="bglA"
                     /locus_tag="VW411_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEU9793148.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTAFRTSIQWSRIFPQGCGKVNTQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            complement(37156..39009)
                     /locus_tag="VW411_000350"
     CDS             complement(37156..39009)
                     /locus_tag="VW411_000350"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEU9793149.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDDYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            complement(39022..40281)
                     /gene="rseP"
                     /locus_tag="VW411_000351"
     CDS             complement(39022..40281)
                     /gene="rseP"
                     /locus_tag="VW411_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEU9793150.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PEMAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPKDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            complement(40303..41106)
                     /locus_tag="VW411_000352"
     CDS             complement(40303..41106)
                     /locus_tag="VW411_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU9793151.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIILMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            complement(41115..41873)
                     /locus_tag="VW411_000353"
     CDS             complement(41115..41873)
                     /locus_tag="VW411_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEU9793152.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            complement(42104..42676)
                     /locus_tag="VW411_000354"
     CDS             complement(42104..42676)
                     /locus_tag="VW411_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU9793153.1"
                     /translation="MNTVKNKQEILEAFRESPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTAVGLRLNEELDFELYVPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            complement(42657..43628)
                     /gene="ruvB"
                     /locus_tag="VW411_000355"
     CDS             complement(42657..43628)
                     /gene="ruvB"
                     /locus_tag="VW411_000355"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU9793154.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNDLEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDVITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            43790..43882
                     /locus_tag="VW411_000356"
     CDS             43790..43882
                     /locus_tag="VW411_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEU9793155.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            complement(44634..45890)
                     /gene="ltrA"
                     /locus_tag="VW411_000357"
                     /pseudo
     CDS             complement(44634..45890)
                     /gene="ltrA"
                     /locus_tag="VW411_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(46684..47100)
                     /locus_tag="VW411_000358"
     CDS             complement(46684..47100)
                     /locus_tag="VW411_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9793156.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQTEMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVFVQDLIVLPSYQRQGIGSSLMKEALGNFKEAYQVQ
                     LATEETEKNVGFYRSMGFEILSTYDCTGMIWINREK"
     gene            complement(47112..47807)
                     /locus_tag="VW411_000359"
     CDS             complement(47112..47807)
                     /locus_tag="VW411_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9793157.1"
                     /translation="MPVNEYGQMIGESMEAYTPGELPSFDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPREMWTYLFQESVADMEELVSLLNQMLARKDRFYYAIIDKATGKALG
                     TFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLACYVFEELNYRRYEWKCDAL
                     NLPSRRAAERLGFIYEGTFRQAVVYKGRTRDTDWLSMIDKDWPQVKARLEIWLRPENF
                     DKNGRQHKSLREL"
     gene            complement(48002..50503)
                     /locus_tag="VW411_000360"
     CDS             complement(48002..50503)
                     /locus_tag="VW411_000360"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEU9793158.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDTSKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVAWLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KVYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVIAVPNKLVNIVVK"
BASE COUNT        14861 a        11661 c         9678 g        14694 t
ORIGIN      
        1 taaacactta gaaataataa aacaaattaa atattatttc taaatatttg aaaataacat
       61 ctatttgtat tatactatct ttgaggtaac tattatgaac tatatcaaaa gaccacatta
      121 tttagatttt ttaagaaaac atcgtgaccg accaatcatc aaagttgtga gtggagttag
      181 acgagctggt aaatctgtgc tttttcaact ctataaagag gagttactag caactggggt
      241 agacgaggat cagattatat tcatcaattt cgaagatttg agttactatg atctgcgaca
      301 ttttcaaaca ttattcgctt atataaaaga tcaattagtt agcaagaaaa catactatat
      361 ctttttagat gaaattcaat atgttgaaaa atttgaactg gtagcagata gtctattcat
      421 cttagcaaat gtagacctct atttgactgg atctaacgcc tactttatga gtagccaatt
      481 agcaacaaac ttgactggtc ggtatgttga gatagaggtt cttcctttgt catttgaaga
      541 atatctatca ggtcaatctc tcacagagaa tctgaataca acagaaattt ttaacaatta
      601 tctctttagt gctttccctt acttattgca aacatcatct tacgatgaaa aaattgacta
      661 tctcagagga atatataact ccatactgtt aaatgatatt gtcactagat tgggaaaacc
      721 aaatcctact attattgagc gcattgtccg aacccttctc agtagtacag gtagcttaat
      781 atcaacaaat aagattcgca ataccctagt cagccaaaat gtttcaatat cccataatac
      841 tttggaaaat tatttgacaa ctttgacaga tagtttactt ttttattccg ttccacgttt
      901 tgatgtaaaa ggtagagcat tattgcaacg tttagaaaaa tattatcccg ttgatttagg
      961 tttacgacat ctcttattac cagaccagaa agaagacatt aggcatatct tggaaaatat
     1021 ggtatatttg gaattgagac gtagatattc acaagtatat gttggtaatt tagataagta
     1081 tgaggttgat tttgttgttg taactgatct tggccactac gcttattatc aggtcagtga
     1141 aacaacactt gctccagaaa cactagaaag agaacttaga ccactagaag ccattaaaga
     1201 tcaattccct aaatatctat taacaatgga tacgattcag ccaacagcca attacaatgg
     1261 aatcgagaag aaaagcatta tagattggtt actagaaaaa tagataaata taaatcatac
     1321 agctaattag atttgcaaca gtctgttatc aatgattcta cccaaatcct aacaagatat
     1381 agtgaatttc gaatacgcta tataatacgg acacttgaaa atagaaattg gggatgaaag
     1441 gggatctata atttctggaa gtactatcaa aaattaatat catagtctta ttagagaata
     1501 gcatcaccca ctttctcaaa taagattaaa ttgtaactga attataatga aaaagagact
     1561 gagcaatcag tctttaaaat cagaaaagcg catagtatca ggtattgaac aaccttgata
     1621 atatgcgttt tattatggaa atatttgctt cattttctcc tgaaatagag cttttgctat
     1681 cctatttttc tctatttcta atgatttact tcaacttctt acctcttggg aaaaacaaca
     1741 aagatatgaa ctagcaaagt ttggcatcct cagtaatcca gaacaatttg tttttacata
     1801 tatcgacaca aaaggaaaca tcaacaagcc tttacatgcc gactatctga acaataaaat
     1861 gaaaagcatt agaaagcgac ataaagaact gacacacgct acaccgcata aattacgaca
     1921 tacaggagcg acacttgcta aaaaagcagg aatgagctta gaagccattt ctgaagcatt
     1981 aactcatagc gataccgtta caactaagac ttatgttaat acttcaaaca tagttccatt
     2041 atctgctgga caagttgcct accaacatct taaaaataag tagggtaatt ttagggcaaa
     2101 ttttagggta aactttttta gaaagtatag aaaaaagcac ccatagggta aacccttgag
     2161 tgctttgaaa cgttgatata atcgtattga ttaacgtttt gaggcaactg accatgtcag
     2221 gttgcagcca catttgtaag cgactaaagt cgcaacaact gtgtcaattg caccaattta
     2281 gtaagaaagt ataaaaaaag aacaccccga aaggtgctct gtataagtat agtaattctt
     2341 tcgaattaac gtttactaaa ttgtgatgct ttacgagctt tcttaagacc tggtttctta
     2401 cgttcaactt tacgtgagtc acgtgtaaga agtcctgcgc gtttcaatga atcgcggaag
     2461 tctgggtcta cttgaagaag ggcacgagcg ataccgtgac ggatagctcc tgattgacca
     2521 gcgtatccac cacctataac gttaacgaaa acgtcgtatg aacctacagt tgaagtaact
     2581 gcgaatggtt ggttgatgac aagacgaagg tcagcgtgtg ggatgtactc ttcaacatct
     2641 tttttgttaa cagtgatttt accagttcct ggaacaaggc gaacgcgtgc aacagcgttt
     2701 ttacgacgtc cagtacctgc atattgtgct tgtgacatac tttattgttc ctttccttag
     2761 ataagtcctg aaatgtcaag aacttctggt tgttgtgcag cgtgagtgtg ctcagctcca
     2821 acaaatactt tcaacttcat accttgagcg cgtccaagtg tattgtgtgg aagcatacct
     2881 ttaactgatt tctcaatcaa acgtactgca tttttagaac gaagttcacc tgcagagatt
     2941 tgtttcaatc cacctgggtg gtttgagtga gtgtagtaga ttttatcagt tgctttttta
     3001 ccagtcaatt taactttttc agcattgata acaatcacga agtcacctgt atcagtgtgt
     3061 ggtgtaaatg ttggtttgtt ttttccgcga agtacgctag caactactgc agaaagacgt
     3121 ccaagtggta catcagttgc gtcaactacg taccatttac gttcaacttg gcctggttta
     3181 gccataaatg ttgttttgtt catgatttct cctatatata gttcgatttt tgtttacggt
     3241 gatgggtgtt ccttcccatc gaaaagtatt tggaaggttc cggggccttt caaatggggt
     3301 aaacaatacc gcctactatc ataccaaatt ttacccctaa aagtcaatag atatgaaaaa
     3361 tatttttcgg taacttctca aagtaactga aagttatata atcaagttaa tttgggaatt
     3421 tacctattaa cttcggtaga tttttaggaa ttttttccct atttctccct gtaactaaaa
     3481 tccttttcaa aaaatcacct tttcttgatt tttaatccta tttgctgtat gataagggaa
     3541 aagaaagggg acagagatat ggcttttacc aatacccaca tgcgatctgc tagttttggt
     3601 attgttacca gcttgcctga tgacatcatt gactcttttt ggtatatcat cgaccatttc
     3661 ttaaaaaatg tctttgaatt ggaagaagaa ctcgagtttc aattgcttaa taaccaagga
     3721 aagattacct tccacttttc aagtcaacac ctccctacag ccattgattt tgactttaac
     3781 catcctttcg accctcgtta tcccccaaga gtactggttt tagacatgga cggtagagaa
     3841 actatcctcc tcccagaaga aaatgaccta ttttaatact caatgaaaat caaagagcaa
     3901 actaggaagc tagccgcaag ctgtacttga gtacggtaag gcgacgctga cgtggtttga
     3961 agagattttc gaagagtata aaaactctag ccttcagttg caagtaactg aaaactagag
     4021 tttttctatt ttttcaaagc atcatacaag ttgcggatcg gttgttttaa tatcggatgg
     4081 ataaaatgag gcgcaatttc ctgtaaggac tcaaggacaa aaaggcgttc cgctatgtaa
     4141 ggatgaggca atatgaggtc gtctgtataa aggatctggt cctccacaaa gagcaagtcc
     4201 aaatcaatca aacgaggtcc ccaatgcact tctctcaccc gtcccagctc tgactcaatg
     4261 gctaacaagg tttctaacaa gtcttgtgct ggtagccagg tttccacctc aaccacttga
     4321 ttggcaaagc tatcctgctc cactccaccc caaggctccg tcgctaagac actggactct
     4381 ttgagaatat ggatgccacg agctcgcagt ttgtcaatgg cttgcttcaa gtttgcttgt
     4441 ttatctccca tattgcttcc tagggcgata aaggctcgtt gcttgcggcg atgaatggtt
     4501 accgagcaag tatctagtga caaatgcacc ggtgcccaag gttttttcag ttccaacttc
     4561 atttcttgga caagaggata aaactcaaag gtacgttcca ccagtttata ggctaccgtt
     4621 tcaatcaaat cttcactcgt ttcctgaaac caagtcgtcc actgctgaca caattctccg
     4681 taatggacag aggctgttaa atccaagtct gtagctgcct tggtcatatc ataggatagg
     4741 atggctgaaa cgacaaattt ctgccccaat tctttctcac taggaaaaag accatgatag
     4801 gcaaacattt ccaaatcctt aatctgcagt tgatccataa tgattccttt ctagaaaaat
     4861 ccgcttgaag cggattcttt ttatagcccc attaaacgat aagcttggtc acggagatcc
     4921 ttatctgttt caaatagacc acgagctact gtcgtcaagg ttgcagtgcc tggttttcta
     4981 acaccacgca tactcataca catatgttcc gcctcaatga caacaaaggc tcctttagca
     5041 cctagatagt ccatcaaggc atcggccact tcgatattca aacgttcttg aatttgtggt
     5101 tttttcgaat aaacttcaac cgtacgggct agcttagaca agcctgccac acgaccatct
     5161 ggaatgtagg caatgtgcgc tctaccataa aatggcaaga agtggtgttc acacatggta
     5221 tggaaaaaga tatccttttc taccaccata ttatcgtcaa taatttcaaa ggattttgac
     5281 aaatgttcct ctgctgtttg accaagacct gaaaaaatct cttgatacat acgggctaca
     5341 cgagcaggtg tttcctgcaa gccctcgcga ttagcgtcct ctcctacagc ctcgataatc
     5401 atttttacag ccgcttcaat cttttgtgta tccattctcg ttcttcctct ccatcagata
     5461 ggccctcact tggctcaaga aatacaagga acctgtgaca atcctaactg tttgtttctc
     5521 ttctttttta tctgtcaaat tctgctctag aaaatcatgc caatcttggt agctgagatt
     5581 tctagactta gctgcctctt tcagcacgtt ttcatccgtc gcccgactat ccgcaaaatg
     5641 tgttagagta agctcggtaa ctggcatggc tcccagcaag tccaacatat cctccaaggc
     5701 cttggttttg atacaagtga agaggatttc cttatgataa tccgcaaaac gttcttgcaa
     5761 ggttaccaac aaggccttga tagcatgggg attgtgggct ccatccaaaa tcatcaaggg
     5821 atctcttgac acgatttcca aacgccctgg ccaacttgtt tcttccaagg cttgaccaag
     5881 aaaatcattg cttgctagct ctcgaccatc ttcttgacaa aaagtatcaa gtaaagctat
     5941 ggccatccca gcattctcta tttggtacaa accaagcagg ctagtctgga agcgaccttg
     6001 tctgacagca cttgtatagt caaagacttc ccctgtcacc acactttctt gatgacgaac
     6061 ctgataatct gtcccgtagg caagtctcgg cgcatctttc ccttccgcaa tgcggtcaat
     6121 cacagccaag gcttctggag caatacgccc tgttaccaag ggcatccctt gtttgataat
     6181 acctgccttc tgctctgcta tgacctccaa ggtgtcacca agtagagcca catgatccaa
     6241 gccaatagtt gtaattcctg tcaaaatggg ctgacagaca ttggtactat ccaaaagtcc
     6301 acccatgcca acttccatga tggccacatc tacttgctct gaggcaaagt agtcataggc
     6361 cagggctgtg ataatctcaa actcggttgt gccctgtaaa ttggcgaccg cttctccctc
     6421 cagcaaagac tgatagtctg ccatgagagc ttctagcctc gcttctgaga tcgattcccc
     6481 attgatgcta atctggtctg tgtaatgaat gagatagggc gagctaaaca cgccaactct
     6541 caaccctagc ttttctagca tctttttcaa aaaagcaata gtcgagccct tgccgttagt
     6601 ccctccgata tggaggacct tgagtttgag atggggattg ccacgcaaag ctaacagttc
     6661 caccattcgt tccaagccaa aatgcggttg atccgtccgg tagttagcaa tccactgatt
     6721 gttttcaatt tctttcatct tatttatatt gttttaaatc taaattttcc gcttcatcag
     6781 ccagacgaat ggcagaggca atttcaactg ccatcctgtg actagctacg tcatgcacgc
     6841 gcaccacttc tacaccctgt ctcacagcga tactagttac atgagccgaa gccgtgtccc
     6901 gatttcggaa accaagctct gtctcaggat tgacttcaaa accattctcc tctaggatat
     6961 tgatgacaaa tcgcttgcgc gacactccga gaaagattgg atagcccttc tgatgtagtt
     7021 tatccaggtc ccgtaaaaga agcagatttt ctttcttggt cagaccaaag ccaattcctg
     7081 gatccaacag gatattttct ggtgcaatac cagcttctgc cgctctcgct agtgctcgtt
     7141 caaagaaagc caccatcaag tcttcgattg gcaatgtttc aaagtcagct aactcttttt
     7201 ctgtaaaggt ttgaccaaaa ccaaaatgag ggaagataag cgaactagga tgctgaggtc
     7261 gagccataac tgggttaaac atgatgacca ctttcgctct cgcttcagct accacataag
     7321 ccattttctc atcacccata agaccagtga tatcattgac tagatcggca ccagcagcca
     7381 aagcagcctc tgctacttga ctcttccaag tatcaataga gatgaggaca tcactttcct
     7441 tgcgaatcgc tttgatcact ggaacaacac gctggatttc ctcttctatc tcaacatagc
     7501 tacttcccgg ccgagtcgat tctccgccga tatctagcat actggctcct tctgctatca
     7561 atttacgagc ctgctggagc gcctgctcaa gagcaaaaaa ttgaccaccg tccgaaaagg
     7621 agtctggggt tacattgata attccgcaaa taactgtctt tgcatgattg gctttacttg
     7681 acatatcggt cactccctca aggattttca tcatattatt tttctatttt accataaaaa
     7741 gaaaaagatg gacacgattg cattcatctt tccctggtag aaagaggtcg gttcctacat
     7801 aaaaactttt aaacagaaat tcctaatgtc cgactcataa tcaccacaag agccaacaaa
     7861 cagaaagcaa tcccattaac aatcatgtga agcaagatcg acatttccaa acgttgggtc
     7921 ttgtaggctg tccaagatag aactgtcgac atacctccat aaatcaataa agaaggtaaa
     7981 ttacttggtt gatgcaataa agcaaacaca atcgtaccga ctacaaatcc caagttctcc
     8041 ttgcctcgga aaatcttttt aggaacaatc ccacgacaca agatttcctc acaaatcgga
     8101 gcaagcaagg ctagcaagaa gaaactggaa atcaacgaac tattttgaac catatcatta
     8161 atctgagact ggttagctgt tgtcgtctca tttgacagtt gcaataaaat ggaaccaagt
     8221 atatttgacc cgacaataac tagataactc aagcccaaac gtgccaaatc tttagctcta
     8281 aaaaaagaaa aattaaaact agctaacttg gttttacgag ctcccataat aaatagagcc
     8341 agaaccacaa ttgaaaggcc agcaactatc agccctgact gtagcagggc tacttctttt
     8401 aaagtcaaaa tagaggtaac cactagggga acctgatata aaaggacggc aagtaaaaag
     8461 accaaaatcc agcctgcacg attcaacaat tctttccaca tatttttctc tttcatcacc
     8521 tatctccttt gtattaaaaa aggggggaaa tccccctggg tcattctatt ataaggccat
     8581 gctgatgtag ttaaggataa acaaggcatc caaaatccaa atcatgacat gaacatcttt
     8641 aacttgacct ttaacaagct tagtcaaagt gtaagtcaag aaaccaactg caatcccttg
     8701 agtgatagag tagctgaatc ccataaagat agatgtgaag aaggcaggaa ctgcttcaga
     8761 catatcatcc caatggatat ttttcaagct accaagcatc ataatcccaa cgataatcaa
     8821 gattggagct gtagccgctg ttggtacgat cgctagaagt gggctaaaga agcttgaaat
     8881 cgcaaaacag atagctacaa ccaaggctgt caaaccagta cgtccacctg caccgatacc
     8941 agcagcagac tcaacataag tcgttacgtt tgaagtacct gcaatggcac cgactgtcgt
     9001 tccaatcaaa tcagagtaaa gagccttatc caatttggct gattggtgat tttcaccatt
     9061 tgtcgctacg ataccaactt tttcacctgt accgatcaag gtaccaattg tgtcaaaaat
     9121 atctgtcaat gagaaggcaa gaatggccat cagagtttca ggcaagcgag ctgtatctga
     9181 aaccaaagct cccaatcctt ctgaaccaag agctgcacca aagattgtct tcaaatcttc
     9241 aaaagctgca ccaacatggt tattagcaaa atcgatacta gacaaatcaa ccaaaccaac
     9301 tgcaatagca agaacagttg ttgtcaagat agagagaata attccccctt taatcccttt
     9361 gatgacaaag aagatagtaa tggcaagtcc tgcaagagcc actaaaacag ctggattatt
     9421 aaagctgacc aatcctggaa ctgctgaaga gtttgctgca atcgttgctt gagctttgtc
     9481 agccccttct cctacaacag tatagttgcc tggatcaatc gtgaatttca aaagtccagc
     9541 attcttaatc cctacatagg caaggaagac accgatacca gctgaaatag ctgagcgaag
     9601 agcattggga atcgattcaa tgatcatttt acgaacattt gtcaaggtaa taatcaatga
     9661 aataatccca cagatgaaga ccatagctag ggcttcttgc caagaataac caagcccgaa
     9721 tacaactgta aaggtaaaga aggcattgag tcccatacct ggcgcttggg cataaggtaa
     9781 gttagcataa aaagccatca tcaaggtacc cgctactgca ccaataatcg tcgctaggaa
     9841 gacgccctga gcaggcattc ctgtttgtga aagtatttgt gggtttacaa agagaatata
     9901 gctcattgca aagaaagttg ttaaaccagc gagaacctct gtacgaacgt ctgtaccgtt
     9961 ctcttttagt ttaaataatt tgtccatcat caatctcctt ttaattttta cgaacaataa
    10021 tagaattata tcatttatca ttcacttttt caatgtcttt gtttgatttg tttaagaaat
    10081 taatccaatt tcattttttg tttggaatct gcttttctgc ctgctttctg ttataataga
    10141 agacatctta tctgaaaaca cactagaaag gtccatatga cgaaaaaaat tattgcagtt
    10201 gacctggatg gaaccctgct caactcagac agtcaaattt ctgactttac caaaagaacc
    10261 atcaaaaaag ttgctgaaaa aggccatcag gttattatta cgacaggtcg cccttaccgt
    10321 atgtcaaaag atttttaccg tgaactgggc ttagacactc ctatgattaa cttcaacgga
    10381 tcccttactc atttaccaga ccaagtttgg gattttgaaa agtgtttgac tgtagacaaa
    10441 aaatatctgc tagatatggt tcaacgttca gaggacattc aagccgattt tatcgctgga
    10501 gaatatcgta aaaaattcta cattacaaat cccaatgaag aaattgccaa tcccaaacta
    10561 tttggtgtag aagctttcca gcctgaagat caattccagc ctgaattggt gaccaaggac
    10621 cctaactgta tcctcttgca gactagagcc agtgacaaat attccttggc aaaagaaatg
    10681 aacgccttct accagcatca actttctatc aatacctggg gaggtccgct caatatcctt
    10741 gaatgtaccc caaaaggtgt caacaaggcc tttgctttgg actacttgct caagataatg
    10801 aatcgtgaca aaaaagattt gattgccttt ggagatgaac acaatgatac cgaaatgctc
    10861 gcttttgctg ggaagggtta tgccatgaaa aatgccaatc cagagctact cccttatgca
    10921 gatgagcaaa tttcccttac caacgaccaa gatggggttg ccaaaaccct acaagactta
    10981 ttcttataac ctatactgat actcaatgag aatcaaagag caaacttagg aagctagccg
    11041 taggttgctc aaagcactgc tttgaggttg tagatgaagc tgacgtggtt tgaagagatt
    11101 ttcgaagagt atgaccagct tgcaagctgg tctttgtgct cttttcacag tctcatcaac
    11161 cagttaatcg attgttctac tttttgtctc caaaaccttg gaaaaggctt tcttttgtga
    11221 tatacttcac atataaatac aagagagctc gtcacactcg actctgttga caaaaaatca
    11281 atccagtccc gttgtccctg ttctcggtca atatcaagga ggatagctat gaaagctgtt
    11341 gttgtaaatc cagaaagcac tggtgttgct attgaagaaa aagtactccg tccacttgaa
    11401 actggggaag cacttgtaga agttgaatac tgtggcgttt gccacaccga cctccacgtt
    11461 gctcatggtg actttggtca agtcccagga cgtgttcttg ggcacgaagg tatcggtatc
    11521 gttaaagaga ttgctccaga tgtgaaaagc cttaaagtcg gtgaccgcgt cagcgttgct
    11581 tggttctttg aaggatgtgg cacttgcgaa tactgtacaa ctggtcgcga aaccctttgc
    11641 cgtacagtga aaaatgctgg ctactcagta gacggtggta tggctgaaca atgtatcgta
    11701 actgctgact atgctgtcaa agttcctgac ggacttgatc cagcccaagc ttcttctatc
    11761 acatgtgctg gagtaacaac ctataaagct atcaaagaag caaaagttga accaggccaa
    11821 tgggttgttc tttacggtgc tggtggactt ggtaacctcg ctgttcaata cgctaaaaaa
    11881 gtattcaatg ctcatgttat cgcagtcgat atcaacaatg acaaacttgc ccttgcaaaa
    11941 gaagtaggcg ctgacattgt gattaacggc ctcgaagttg aagatgtagc tggactcatt
    12001 aaagaaaaaa ctgatggagg agctcattca gctgtcgtaa ctgctgtgtc taaagttgcc
    12061 ttcaaccagg ctgttgactc cattcgtgct ggtggtcgcg tcgtcgctgt tggtcttcct
    12121 tctgaaatga tggaactcag catcgttaaa acagtcctcg atggaatcca agtcatcggt
    12181 tctcttgtcg gaactcgtaa agacttagaa gaagccttcc aatttggtgc agaaggtctg
    12241 gtagtcccag ttgttcaaaa acgtccagta gaagatgctg ttgccatttt cgacgaaatg
    12301 gaaaaaggcc aaatccaagg acgtatggta ctcgacttca cccactaatc taatagaaac
    12361 ctattttaaa agttggaagc ttcttccaac ttttttatat taaattttaa ataagaattc
    12421 aagattactc ctcaaaaact caacatatca acatacaaaa ataaaaattt tttaattttt
    12481 tatgataaat agttgctttt tttatgaaaa cggtttatac ttaaacagtc ttaaagtttt
    12541 cttaaatgaa aactaaaaca aaaaaggagg aatgacaata atgagtatcg gaatcattat
    12601 tgcgagccac ggcgaatttg ctgcgggtat tcatcagtca ggatctatga tctttggtga
    12661 acaagaaaag gttcaagttg tgacctttat gccaaatgaa ggtcctgatg atctatacgc
    12721 taagtttaat aacgctgttg ctgcatttga cgcagaagat gaggttctag ttttggctga
    12781 cctttggagt ggttctccat ttaaccaagc tagtcgcgtg atgggagaaa atcctgagcg
    12841 taagtttgcc atcatcacag gacttaactt accgatgttg attcaagcct acacagagcg
    12901 cctcatggac gctgctgcag gtgtagaaaa agtcgctgct aatatcatta aagaagccaa
    12961 agatggcatc aaagctcttc cagaagagct aaatccagtc gaagaagttg caagcgctgc
    13021 agctgctcca gttgcccaaa ctgctatccc agaaggaact gttatcggag acggtaaatt
    13081 gaaaatcaat cttgcccgtc ttgacacacg tctacttcac ggtcaggttg caactgcttg
    13141 gactccagat tcaaaagcaa atcgtatcat cgttgcttca gataacgtgg ctaaagacga
    13201 ccttcgtaaa gaattgatta aacaagcagc tccaggtaat gtcaaggcta acgtggttcc
    13261 aattcaaaaa ctgattgaga tttcaaaaga cccacgtttt ggagaaacac atgcccttat
    13321 cttgtttgaa acacctcaag atgcccttcg tgccatcgaa ggcggcgtgc caatcaagac
    13381 tcttaatgtt ggttctatgg ctcactcaac aggtaaaaca ttggtcaata ccgttttgtc
    13441 tatggacaaa gaagacgttg ctacatttga aaaaatgcgt gacttgggtg ttgaatttga
    13501 tgtccgtaaa gtaccaaatg attctaaaaa agatttgttt gacttgatta acaaagccaa
    13561 tgtcaaataa gccattattt atgaaaggat tttaaacatg tctattattt ctatggtttt
    13621 agtagtcgtt gtagccttct ttgcaggtct tgaaggcatc ctcgaccagt tccaatttca
    13681 ccaaccactt gtagcctgta cccttattgg gcttgtaaca ggtcacttgg aagcagggat
    13741 tatcctcggt ggatcgcttc aaatgattgc ccttggttgg tcaaatatcg gtgctgctat
    13801 cgctcctgat gctgcacttg cttctgtcgc tgctgccatt atcatggttc ttggtggtga
    13861 ctttaccaag actggtatcg gtgttgccca agcggttgct atccctcttg ctgtagctgg
    13921 acttttcttg acaatgattg ttcgtacaat ttcagttggt ttggttcata ctgcagatgc
    13981 tgccgctaaa aaaggtgact tcggcgctgt ggagcgtgcg catttcatcg cgctactttt
    14041 ccaaggactt cgtatcgcgc ttcctgcagc tcttctcctt atggtaccaa ctgaaactgt
    14101 acaaagtatc cttagtgcca tgccagactg gctcaaagat ggtatggcta tcggtggtgg
    14161 tatggtcgtt gccgttggtt acgccatggt tatcaacatg atggcaactc gtgaagtatg
    14221 gccattcttc gctcttggtt tcgttctcgc tgctgtgtca gatattactc taatcggatt
    14281 cggtgctatc ggcgttgcta tcgctcttat ctaccttcac ctttctaaaa ctggtggaaa
    14341 tggtggcgga ggagccgcaa cttctaacga cccaatcggc gatatcctag aagactacta
    14401 agataagaaa ggactgaaaa catcatgact gaaaaacttc aattaactaa atcagatcgt
    14461 aaaaaagttt ggtggcgttc aaccttctta caagggtctt ggaactttga acggatgcaa
    14521 aacttgggct gggcttatac actcattcca gctatcaaaa aactctatac taaaaaagaa
    14581 gatcaaatcg ctgctcttga gcgtcacctt gagttcttca acactcatcc atacgtagct
    14641 gctccagtca tgggggttac tcttgcgctt gaagaagaac gtgctaacgg tgtggaaatc
    14701 gatgacgctg ctatccaagg ggttaaaatc ggtatgatgg gacctcttgc tggtatcggt
    14761 gacccagtat tctggtttac agtacgccca atccttggat ctctcggtgc ttcacttgcc
    14821 cttactggca atatcttggg gccactcctc ttctttgttg catggaactt gattcgtatg
    14881 tcattcttgt ggtatgttca agagattgga tacaaggctg gatcagaaat cactaaagat
    14941 atgtctggtg gtatccttca agatatcact aaaggagctt ctatccttgg gatgttcatt
    15001 cttgctgtcc ttgttcaacg ctgggtaaat attaaatttg ctttcgatgt ttctaaagtt
    15061 caactagatg aaaaggctta tatccattgg gataaattgc cagaagggtc taaaggtatc
    15121 caagaagcat tcgcacaagt aggacaagga ttgtctcaaa ctcctgaaaa agttactact
    15181 ttccaacaaa acttggatat gttgattcct ggattatcag gactactcct tactttactt
    15241 tgcatgtact tacttaagaa aaaagtatct ccaatcacta ttatccttgc cctcttcgca
    15301 gtgggtattg tggcacatgt tcttcacatc atgtaatcaa gcaactaaaa aggaaccagg
    15361 ttctaaaatc tgattccttt tttctatgct tttattcagc caaggctccc attggatccc
    15421 atggtgcaag tacgattggt tctgctccat aggcagcttg ttcttctgct gtcagcaatt
    15481 ccttacgaac aacgatttgg tatgtgtatt cgtccatcca agcgtctgag gcaacaaagt
    15541 aaccatctgt accgaccttg tctccccatg agttttcaac cttccacttg gttgatttac
    15601 cattttcgtc caagtcaaca cctgtcaaga ccatggcgtg ggtcatcaag ctttcactat
    15661 agtccaaacg tccagccttg tcttgagtaa gtttaatgtc catgcttgat tcaaagtcat
    15721 aaacatctgt cgcaaggatg ccagccttac ggttgctgag ctggccgaca tcagaaccaa
    15781 accaaacagt ctcacctgct tgcatttggg caatcgccaa ttctttcaag cgctccattg
    15841 gaacgttgat gtagcgaact gcacggctac caaccacatt ccccaacatc tcaactgtgt
    15901 aagattttcc gtaaggttta tcagcagttg gagcattgat aacagaaacg tagtcttcta
    15961 aaggaagatt gacatatttc ttgtaaaact cttgtggtgt gattcctttt tcacttttgt
    16021 agttgttatc tttatcgcga taagcaaagt caaacttgcg tggtggaagt cctaatgaca
    16081 tagcaagaaa gttaaagatt tcttgcaaga ggtcttcttt cttagcttga acagtcgctt
    16141 gatctgcacc agaaacaagc aagtcacgca agatttgagc atcttgacga agcaatttat
    16201 taaggatcgc atttagctca cgactgctgc tagatgaaac agactcagga taaactgact
    16261 taggcacgac accgtatttt tcaaagaggg aaacgaccat atcccattga ccgccatctt
    16321 gttgaggtgt ttggagtaag aagctaacct tgcggctagt caattcttgg tctgaagtcg
    16381 caatgacttg ctccaagaac cagtttgatt tctcatactt atcccagaag aaagtgtggg
    16441 cttgtgacaa ctcaaagttc tccaatttgt attgcgagat gagtttgtgg cggaaggtgt
    16501 tgagagccgc aaacatccag caacgaccag acgctttctg gttagtgacc ttgtccttgg
    16561 ttaaatccaa tgagaaaaca ggtgtgttgt ctacatggct ttggcgacgt tccagagctg
    16621 caaaaattcc gttgtggctg gcagcatttt caatcgcttg gtattttaca tttgcttcat
    16681 agttggcaaa tagtttatca gtaaatgatt cttgaatcgc gttcatagat tcctcctttt
    16741 attctacagt ctattataac tcttttcaca aaggaagcaa ctaaaaaaca taaaaggcga
    16801 ggacattgtc cctcaccttt ttcgaattta tactcttcga aaatcaaatt caaaccacgt
    16861 caacgtcgcc ttgccgtact caagtacagc ctgcggctag tttcctagtt tgctctttga
    16921 ttttcattga gtattaaact aaattaaata atattagcgc ggagaatttc taattcttcc
    16981 ttggtcaagc gacgccattc ccctcgttct aggttctcat ctaatactaa agttcccata
    17041 gtcaatcgtt gcaagtccac cacttccttg ccacagtagc ccaccatacg cttgatctga
    17101 tgaaacttcc cttctgcaat ggtcacacgg atttggcttt gattcttttc tgtatctatg
    17161 gatacaagct ccagtatagc gggttgacag gtaaagtctt tgagaggaat accctcagca
    17221 aatgtctcca catcttcttg ggtcatgatt cccttgactt gtgccagata agtcttgtcc
    17281 acatgacgct tgggcgaaag aagaacatga gccagctgac catcattggt caagagcaaa
    17341 agaccatgcg tgtcaatatc caagcgtcct actgggaaaa cttccttact ccgcgccaag
    17401 tcatccaaca agtccagaac ggttctgtgc ttgggatcct cagtcgctga gataactcct
    17461 ttgggcttgt tcatcatgta gtagacaaac tcttcatact ccaacacttg cccatcaaag
    17521 cgaatctcat ctattttttc atcaatctgc aatttagctg atttttcttt tttaccattt
    17581 acagtcacgc gcccagcctt gagcaagttt ttgacctcag tccgacttcc caccgcacag
    17641 gcaactaaaa atttatctaa tctcatagaa ctattatatc atatcaaaag gaggctagta
    17701 caatgaccaa cctccttttc gtttcatact cttcaaaaat ctcttcaaac cgcgtcaacg
    17761 tcgccttgcc gtatatatgt tactgacttc gtcagttcta tctgcaacct caaaacagtg
    17821 ttttgagctg acttcgtcag ttctatctgc aacctcaaag cagtgctttg agcatcctgc
    17881 ggctagtttc ctagtttgct ctttgatttt cattgagtat cagatttagg aaattaactt
    17941 cctcgtctcc aaaaaatagc taaaacaatc atggctccta aaatcgctgg gataatagct
    18001 gttcctgata aaactggccc ccaagttcca aagagcaagt gacctagaaa ggctccgatc
    18061 caaccgagaa acatttttcc aaaacatccc attcgctctc cacgattggt catagcacct
    18121 gctaaaaatc ccactaggag accaacgaac atacttccta acatattatc tccttaattt
    18181 gcccaattcc cattacggaa aagaggtacc cgcgttccat cctcacgaat accatcgata
    18241 tccatttggt tagaaccaat cataaagtct acgtgaacat ctgaacggtt aagccctgca
    18301 gcttcaagct cctcttcgct catctccgct ccatcaacaa cgctagtcgc ataggctgca
    18361 ccgatagcca agtggtttga cgcattttca tcgaaaaggg tgttaaagaa ggtaatgcct
    18421 gactgagaaa ttggacttgg atctggtacc aaggcacatt cacccaaggc acgcgcaccc
    18481 gcattttcaa agacaaggtc tttcataacc tgatcaccct tctcagcagt gatatctacg
    18541 atttgtccat ccttaaaggt caccttaatg ccttcaatga tatttccgtt gtagctaagc
    18601 ggttttgtag aagtgacata accatctgca cgacggaagt caggcgctgt gaagacctct
    18661 tctgttggca tatttggcaa gaattcttcg ccctgtgcat tgacagcacc agctgattcc
    18721 caaacgtggt tctttggcaa accaagtgtt aaatctgttc ctggcgctgt gtagtgaagg
    18781 gctgaaaatt gctccttatt aagcatatcg gccttgctct tgagaatggc tgcatgttcc
    18841 tcccaagcct taacaggatc tgcttcgtag acacggcaag ttttgaaaat ttggtcccaa
    18901 aggaaatcaa ctgcttcttc gtcgctcgca gcatttggga agactttctt agcccactca
    18961 agtcctgctg cagctgctac agtccagcta accttgttag attgagttgc gatacgcata
    19021 ggcttcatgg caagtcccat agctttagca gaagctgaaa gcttgtcagc gtccactccg
    19081 ttcaagacac ctggatcaga tgaacgaact ccaagacggc tagccttatt ctccaagaga
    19141 tagttcatct cagcaatctt gtattctggc acattgtcca aacgctccat cggggcatgg
    19201 aggaatttct cacggttaat cacatcatct gtccactgaa cgatgacctc atgcgcaccc
    19261 aaggcataag cttctttcac gattagatgt gccaattcac gttgctccac atcaatagag
    19321 agagccaaag tgtgaccagg ttgcacgtta attccgttcg caaccaacaa tttcgcatat
    19381 ttttctagat tttctttaaa atttggtaaa accattctgt tttctctttt ctatttttat
    19441 ttttctttca aagcagaaaa gattcctgct aaagcaatag cacctgacaa gagtgctgtc
    19501 gatagaagaa ttgtacgatc agcaagttct aattgatatt caaatacctt cttagccggc
    19561 ttatcttccg caaatattct tagtttcatg agaaccccct taaaatagat taaaataaat
    19621 cactgtgact gcctgtcctc agcaaattta aaatcaattc cgacttgtct actttataaa
    19681 ccaaaagcca atctggctgg gtatggcatt cacgaactcc ttgaaaatgc ttggatgccg
    19741 tcaatgaatg atcacgattt ctggcaggat gttctttttc ttgaaccaga aaattcaaaa
    19801 cttcttctaa taattctgcc ttcaaaccac gcttcatagc caacttaaaa tcttttttaa
    19861 actgtttatg ataacgaatc ttaagcacgt aagtcctcca tcaaatctga gactgattca
    19921 aaagactggc tcatattacg attttggtct aaatccgtta acgcttggag caacttccga
    19981 ttttcgtcta gtctaacatc aaaaggtaat ccctgatatt gaattgcctg acgaaggaaa
    20041 atattaatag ctgttgtcat atccattccc agattactaa acacctgttg ggcctgctcc
    20101 ttaacctcac tatccagacg gatgcttata ctcatctttg acatactctc accctctttc
    20161 catagactat tttaacaaaa aagaaagcta atgtaaatct attggatata cgttagcctc
    20221 ttttaataga ttattaagca attttttaaa acaactcatc aaacaaactc aactggttat
    20281 cctctggcat atttccaaga atacccatct catccatctt ttcaaccaag gttgatgaga
    20341 gtccaccacg cttgcgtagt tctgttttag agaggaattc tccctcttca cgcgcccgca
    20401 ccagttgctt ggcaacgttc tctcccagac catccattgc tacaaatggt gggataaggg
    20461 tatccccgtc gatgaggaac tctgtcgcct gactacagta gagatctaat ttaccaaact
    20521 tgaaacctcg ttcccacatc tcattgacaa tctcaagagt tgtatagaga tcgatttcca
    20581 cattagaggc ttcattgttc ttccgttttt cagagatttc ttccattctg cgcttgatga
    20641 cctccaagcc cgcacccatg gtcttgatat caaaagcctt agcacgaatg gagaagtaag
    20701 cacagtagta ataaatagga tggtgaacct tgaagtaagc tacacgcaag gccatcataa
    20761 cgtaggctgc cgcatgggcc ttagggaaca tgtacttaat tttcccacag gattcgatat
    20821 accactctgg caccttatta gccttcattg cttcgatata gccatttctc tcctcttctg
    20881 aaatctttag ccacaaaccc ttacgtaccc gttccataat ggtaaaggcc atcttaggtt
    20941 ccagacccgc atgcatgagg taaaccatga tgtcgtcccg acaaccgata acagtcgata
    21001 ggtccgctat tccttgctta atcagatcct gagcattccc caaccaaaca tcagtaccgt
    21061 gggacagacc agacagctga agcaattccg caaaggttgt cggatgggtt tcgtctacca
    21121 ttccacgtac gaaatttgtt ccaaactctg gaatccccaa catacccgta ggcgttccaa
    21181 tttgttcagg tgttacccct agcacatcag tcccagaaaa gagtgccatc acgccttcgt
    21241 catccatagg aattttatta gggtcaatac cagacaaatc ctgaagtttt cgaatcatag
    21301 tcggatcatc atgtcccagt acatcgagtt tgaggacgtt ctcatcgata tcgtggaagt
    21361 taaagtgagt ggtctgccat tcagccgtga catcatctgc tggatactgg acaggcgtaa
    21421 aatcgtagac atccatgtag ttcggaataa caacgattcc ccccgggtgt tggcctgttg
    21481 tccgcttgac acccgccgct ccttgagcga ggcgttctac ttctgcatca cgataaaact
    21541 tgccataatc tcgctcgtaa cctttgacaa atccataggc agtcttggca gctaccgtac
    21601 caaccgttcc cgcacggaag gcatattctt caccaaagat atcacgcaca tccaagtggg
    21661 cgctaggctg atcttctccc gagaagttca agtcaatatc aggaacctta tccccatcaa
    21721 aaccaaggaa ggtctcaaac ggaatatcct gtccgttttt actgagtttg tgaccacagt
    21781 ttggacagtc cttatggggc atatcaaatc ctgaaccgta cgaaccatct gtgataaact
    21841 cactgtactg acactgacca cagacatagt gaggagagag aggattgacc tccgtaatcc
    21901 caatcatggt cgcaacgaaa ctagatccga cagacccacg agaaccaacc aaataacccc
    21961 gttcattaga acgttgcacc agcatctgcg atgccagata aatcacagca aatccattcc
    22021 ccagtatgga tgttaattct ttttcaatcc gcaaatcaac aatatctggc agcggatttc
    22081 cataaatctc aaaagctttc ttataggtca actcagcaac tgtttcttca gccttgtcga
    22141 tgaaaggcgt atacaagtca cccttaacga cttcaacgga ttcaaatatt tctgccaagg
    22201 cattggtgtt ttcaataacc agtttacgag ccagttcctc tcccaaaaag gcaaattcat
    22261 ccaacatctc attagtcgtt cgaaaatgag cctttggaag tggtgctggt tgggcatgtt
    22321 caccatgacc gatagttcga ttaatcatcg caccctgtcc caaactacgg acgataattt
    22381 cacgataaat ctcttcttcc ggttcgatat agtgaacatt tcccgtagcc agaacaggct
    22441 tgccaaggcg gtctccaacc tctatcaaac tcttgataat ggtctggagt tcctccatat
    22501 ccttgacctg ctctttggca atcaagggtg catagatagc cggtggcatg acctcgataa
    22561 aatcataata cttggccacc tcaaccgccg catccacacc ttgagaaacg accacgtcaa
    22621 aaacttcacc ctctgaacag gctgaaccta aaatcaagcc ctctcgatgg gcatctagaa
    22681 ccgttctcgg aatccgtggc actccttcaa aatacttggt attagacaag gaaaccagct
    22741 taaagatatt ttttagacct acctgattct tgacatagat ggtcgcatgc ttgatccgag
    22801 cttttttgta agaatctgga ctgattagat caatgttgag tctagctaaa tcggtcacac
    22861 catgtttttc tgctacctct ttgataaaga tgaaaagcag acgaccagtc gcttccgcat
    22921 cgtagttggc catgtggtga tgttccaagg ccacaccaaa acgcttggtc aaaggcccca
    22981 aaccatggcg tttatactca ggatagaggt ttctagcaaa ctccagcgta tcaataactg
    23041 gctgactaat ctttggaaga tcatgccgct cataattagc attcataaag ccaacgtcaa
    23101 aggtagcatt gtgggcaact aggaccgtat ccttgcaaaa ttcttggaat tcttgcaaaa
    23161 cttgttctag tggtttggca tttttgacat gatcatctgt aattccagtt aactctgtag
    23221 taaaggctga caagggatgc ccaggattga taaattcatc aaattcagca ataacattcc
    23281 ccttgtacat cttagaagcc gcaacctgaa tcaagtcatt atagatagct gaaagtcccg
    23341 tcgtttccac gtcaaagacc acgtaggttg cttctgacaa gtccatctcc acttcgttat
    23401 agacgatagg gacacggtcc tccacgatat tggcttccat cccatagatc agctggattc
    23461 ccgctttctt agccgcctta tagccatgtg gaaaggactg gacattccca tggtccgtga
    23521 tagcaaccgc cttgtgtccc cacttagcag ctgttgcaac aatctcttcg acctctggca
    23581 aagcatccat agtcgacatg ttagtatgag catgaaactc aacccgacgc tcaccttctg
    23641 gcatcaaatc cttccgctca tagtgaacaa cttcctgcag atcctgtacg ttcatagtca
    23701 aatcgcgtgt gaagttattc atctccacat tccctcgaac tcggagccaa gaattcttct
    23761 tgatgaggtc aaacttctgg gcctcttcct cgtttttaac ccacttttgc atagaaaaac
    23821 ttgaagtata gtccgtcatt ttaaagttga ttaaaacacg acctgttcta gtcacttttt
    23881 gctccacatc aaaaacaacc ccttcaaata ccagacgatt ttcctctgtc gtcacttcga
    23941 tcataggagt aatctccgcc ttatccagct tgggtttagc tgcagctttt ttcgcttgaa
    24001 aatcaaaggc tggtttctct tccgctggag gaggtgccat ctgctccagt tgttccatag
    24061 cacggagcgc ttcctcattg gcagcttgaa caatctgctc attttcagca tgaaaggcct
    24121 cttcctgctc ttgggtcagg acatcattct tctcgacttg acagttaaaa gttggaaaac
    24181 caaacttttc aagttgtttg gctaaattag gaagatgatt cttcttaaaa tgttccttat
    24241 caatcgcttc agatccttca ataaatagct gattaccctc agcacgaact tgcaaatttt
    24301 gataaaggga cttaaaacct tgactagcac atggaccttc agagaaagcc tccctatagt
    24361 aggactgcaa gagctgattt gaaaattctt gagaccgagc cttaatttca aaaacagctt
    24421 tattgcctgt cttagaaaat tcttcgctca aacctttctt taattctaaa aagatttcaa
    24481 tcggtaaaat attagaaaat acgaaatgaa actcccatac cttactaatt ttatgaacca
    24541 caactcgctc aatattggcc tgtgctaaag caggagcctg tctcatttca gcaggcatcc
    24601 ccaattgatt catcaaaatt tcaaaactat ttgacattca ttttcctcac attattcttc
    24661 tactatttta ccatatttag aggtattttc taaagacaaa aggaagccac taagtgactt
    24721 ccttctagag tgaggacgga ttagtcttca cctttatttt tcttaataat ttcttcttgt
    24781 actgacttag gtacatcttc gtagtggtca aataccatca tgaatgtacc acgtccttga
    24841 gatgcagaac gaagaactgt tgcgtaaccg aacatttcag caagtggaac gtaagcacga
    24901 acgatttggc tgttaccgtg tgcttccata ccatctacac gtccacgacg agcagttacg
    24961 tgacccataa catcaccaag gttttcttct ggaacagtga ttgttacaag catcattggt
    25021 tcaaggatag ctggttgtgc tgatttagca gcttctttaa gggaaagtga agccgcaatc
    25081 ttgaaggcag tttcagatga gtcgacatcg tgatatgaac catcataaag cttagcttta
    25141 acgtcaacca ttgggtaacc tgcaagaaca ccgttagcca tagattctac caaacctttt
    25201 tcaaccgctg ggataaattc acgaggaacc acaccaccga cgattgcgtt ttcgaattcg
    25261 aatcctttac cttcttcgtt tggagtaaat tcaatccata catcaccgaa ttgaccttta
    25321 ccaccagact gacgtttgaa gaatccacgt gcttgagtag aagcgcggaa tgtttcacgg
    25381 taagatactt gaggagcacc tacgttcgct tcaactttga actcacgacg catacgatca
    25441 acaaggacgt caaggtgaag ttcacccata cctgagataa ctgtttcacc agtttcaacg
    25501 tttgtttcaa cgcggaatgt tggatcttct tcagccaatt tttgaagggc gatacccatc
    25561 ttgtcttggt cagctttaga ttttggctca accatcaatt ggataactgg ttctggaacg
    25621 ttgattgact caaggatgat tttagctttt tcatctgtca atgagtcacc agttgtagta
    25681 tctttcaaac caacggcagc agcgatatca cctgagtaaa cagtgtcgat ttcttgacgg
    25741 ctgttagcgt gcatttgaag gatacgtccg atacgttcac gtttaccttt agaagtattc
    25801 aatacgtatg aacctgattg aagaacacct gagtaaacac ggaagaatgt caaacgacct
    25861 acgaatgggt cagtcatgat cttgaaggca agagctgcaa atggctcttc gtcagatgct
    25921 ggacgaattt cttcagcgtc tgtatctggg ttaatacctt tgattgctgg gatgtcaagt
    25981 gggcttggaa ggtagtcgat aaccgcatca agcatcaatt gaacaccttt gtttttgaag
    26041 gctgaaccac acaatactgg gaagaattca acgttgatag tcgctttacg gataccagct
    26101 ttcaattctt cgttagtgat ttcttcacct tcgaggtatt tcatcatcaa ttcttcgtca
    26161 gtttcagcaa ctgcttcaat caatttttca cggtattctt gagcttggtc aaggtattca
    26221 gctgggatgt cttcttcaag gatatccgta ccaaggtcgt tagtatagat ttcagctttc
    26281 atcttgatca agtcaatgat accacggaag tcatcttcag aaccgattgg caattggatt
    26341 gggtgtgcat ttgcttgaag acgatcgtga agtgtgctta cagagtaaag gaagtcagca
    26401 ccgattttgt ccattttgtt ggcaaatacg atacgtggaa ctccgtactc agttgcttga
    26461 cgccaaactg tttcagtttg aggctcaaca cctgattgtg agtcaagaac ggtaaccgca
    26521 ccatccaata cacgaagaga acgttgtact tcgattgtga agtccacgtg tcctggtgtg
    26581 tcgatgatgt ttacgcggtg gttgttccat tgagctgtcg tcgcagcaga tgtgatcgtg
    26641 ataccacgtt cttgctcttg ctccatccag tccatttgtg acgcaccttc gtgagtttca
    26701 ccgattttgt ggattttacc agtgtagtaa agaatacgct cagtagttgt tgttttaccg
    26761 gcatcgacgt gagccatgat accgatatta cgagtttttt caagtgaaaa ttcgcgtgcc
    26821 atgaggtttg tttctcctat ttatttttga tttctattct attataacac gattttaata
    26881 aaaacggata ggcaggacct acccgttctc aatgttttca tgctattgtt ggtttcaact
    26941 tacgagatgg taagctgagt tatagctaat actaatcgat ttagctaatt tgaacccggg
    27001 ctaaagttag ttagccgatg tgagctggaa cgggatgctg cgtgaaaaag ataaaactcc
    27061 ttgtattcgt cgaatactgc gtcagtttcc tatttttacc ttgcatcctt accgttccta
    27121 gcatcatgat ttagttgagc tcaagctaaa agtctggaga aaaagatgaa tctcattggt
    27181 tgcaaccgca acctgcttcg attccctatt ttctctggga ctttcttaac gctttcgcat
    27241 cctatcttac caacggaagt gtgcgaatgc acggttagct tcagccatac ggtgagtatc
    27301 ttcacgtttc ttaactgctg caccagtgtt gttagcagca tccaagattt cttttgcaag
    27361 acggtcttgc attgtgtgtt caccacgaag acgagcgatt gttaccaacc aacgaagtcc
    27421 aagtgttgta cgacgttctg gacgaacttc aactgggact tggtagttag aaccaccaac
    27481 acgacgtgca cgtacttcaa gtacaggcat gatgttttcc atagctgttt caaatacttc
    27541 aagtgcatcg ttgccagtag cttctttgat ttgctcaaaa gcaccgtaaa cgattgaagc
    27601 agcagtacca cgtttaccat caagcataac gcggttgata agacgagtaa ctagttgtga
    27661 attgtatagc ggatctggca atacgtcacg ttttggagct ctatttttac gactcatttc
    27721 tctttatccc ctttccttat gcttttggac gtttagtacc gtatttagaa cggccttgtt
    27781 tacgatcgtt aacacctgca gtatcaagtg caccacggac gatatggtaa cgtacccctg
    27841 gaaggtcttt tacacgtcca ccgcgaagaa gcaccacgct gtgctcttgc aagttgtgtc
    27901 cgatacctgg gatgtaggca gtaacttcga taaggttgct caaacgtaca cgagcgaatt
    27961 tacgaagggc tgagttaggt tttttaggtg tcattgttcc aacacgagtt gcaacaccac
    28021 gtttttgtgg tgaagaaacg tttgtttgaa cttttttatg actattgtaa ccaacgttca
    28081 aagctggtga tttagatttt tctacttttg atttacgcgg tttgcgaacc aattggttaa
    28141 ttgtaggcat ctacattctc ctgtgttttt ttatttttgg tgatgataca cttggtgaca
    28201 gatatcatct gtgtgtactt ttgcaacatt tgtcagcacg tccctgtaca ctcttgagag
    28261 accaaaagta aaaagtaccg tctattattg taacaaaatt ttcccttgct tgtcaagata
    28321 tttttctttt ttattggtaa tgttagagat caatttctga ataaaaatag ctgagaacac
    28381 atcatcttcc taataacacg gcttggctta cgtgacattt ttatctccgt catttatact
    28441 gatatataaa atagtacgga aaacttctca aaagtaagaa tttctttcac aaatcaaaga
    28501 aaacgcttgc atattgtttt actacatgct atactgatat agaaatttat tgattggaga
    28561 atcattatga gaaaaacacc atctcacact gagaaaaaaa tggtctacag catccgttcc
    28621 ctcaaaaatg gaactggttc tgtccttatc ggcgcaagcc ttgtcttgct tgccatggct
    28681 acaccaacta tctcatccga cgaaagtaca ccaaccacta acgaacccaa caacagaaat
    28741 acaaccaccc ttgcccaacc tcttactgat acagcagctg gctctggtaa gaacgaaagt
    28801 gatatttctt cacctggaaa tgcaaacgct tccctagaga aaacagaaga aaaacctgct
    28861 gcaagcccag ccgatccagc accacaaact ggacaagatc gttcaagtga gccaactact
    28921 tctactagtc cagtaacaac tgaaactaag gcagaagagc ccatcgaaga taactacttc
    28981 cgtatccatg tcaaaaaact tcctgaagaa aacaaggatg ctcaaggact atggacttgg
    29041 gacgatgttg aaaaaccatc tgaaaactgg ccaaacggag ctttgtcctt caaggatgcc
    29101 aagaaagatg actacggcta ttacctagat gtcaaattaa agggagaaca agccaagaaa
    29161 attagcttcc tcatcaacaa tacagctgga aaaaatctaa ccggcgataa atctgtagaa
    29221 aaactagttc caaaaatgaa cgaagcttgg ttagaccaag attacaaggt tttctcttac
    29281 gagccacagc ctgcaggaac tgttcgcgtc aactactacc gcacagatgg caactatgac
    29341 aagaaatctc tctggtactg gggagatgtg aaaaatccaa gtagcgctca atggcctgac
    29401 ggaacagact ttacggctac aggcaaatat ggccgctata tcgacattcc tcttaatgaa
    29461 gccgcaagag aatttggatt tttattacta gatgagagca aacaaggaga cgacgtgaaa
    29521 atccgtaaag aaaattataa gttcacagat ttgaaaaatc atagccaaat tttcctaaaa
    29581 gacgatgatg aatcgattta cacaaatcca tactatgtcc atgatatccg tatgacagga
    29641 gcccaacacg taggcacttc tagcattgaa agtagctttt caacacttgt cggtgctaaa
    29701 aaagaagata tcctcaaaca ctccaacatc actaatcacc taggaaacaa ggtaactatt
    29761 accgatgttg caatcgatga agctggtaag aaagtgacct acagcggaga tttctctgac
    29821 acaaaacatc cttatactgt tagctacaat tccgaccaat tcactaccaa aacaagctgg
    29881 cgcctgaaag atgagacata cagctatgat ggcaaactgg gagctgacct aaaagaagaa
    29941 ggaaaacaag ttgatttgac cctttggtca ccaagtgctg ataaggtttc tgttgttgtc
    30001 tacgacaaga atgaccctga caaagtagtt ggaactgtcg ctcttgaaaa aggggaaaga
    30061 ggaacttgga aacaaactct agacagcaca aacaaactcg gaatcacaga tttcactggc
    30121 tactattatc aataccaaat cgagcgtcaa ggtaaaactg ttcttgcact cgatccttac
    30181 gctaaatctc ttgctgcttg gaatagcgac gattccaaga ttgacgatgc ccataaagtg
    30241 gctaaagccg cctttgtaga tccagctaaa ctcggacctc aagacttgac ttatggtaag
    30301 attcacaatt tcaagactcg tgaagacgcc gttatctacg aagctcatgt gcgtgatttc
    30361 acttcagatc ctgccattgc aaaagacttg accaaaccat ttgggacttt tgaagccttc
    30421 attgaaaaac tagactatct caaagacttg ggtgtaaccc atatccagct ccttccagtc
    30481 ttgtcttact actttgtcaa tgaattgaaa aaccatgaac gcttgtctga ctacgcttca
    30541 agcaacagca actacaactg gggatatgac cctcaaaact acttctcctt gactggtatg
    30601 tactcaagcg atcctaagaa tccagaaaaa cgaatcgcag aatttaaaaa cctcatcaac
    30661 gaaatccaca aacgtggtat gggagctatc ctagatgtcg tttataacca cacagccaaa
    30721 gtcgatctct ttgaagattt ggaaccaaac tactaccact ttatggatgc cgatggcaca
    30781 cctcgaacta gctttggtgg tggacgcttg gggacaaccc accatatgac caaacggctc
    30841 ctaattgact ctatcaaata cctagttgat acctacaaag tggatggctt ccgtttcgat
    30901 atgatgggag accatgacgc cgcttctatc gaagaagctt acaaggctgc acgcgccctc
    30961 aatccaaacc tcatcatgct tggtgaaggt tggagaacct atgccggtga tgaaaacatg
    31021 cctactaaag ctgctgacca agattggatg aaacataccg atactgtcgc tgtcttttca
    31081 gatgacatcc gtaacaacct caaatctggt tatccaaacg aaggtcaacc tgcctttatc
    31141 acaggtggca agcgtgatgt caacaccatc tttaaaaatc tcattgctca accaactaac
    31201 tttgaagctg acagccctgg agatgtcatc caatacatcg cagcccatga taacttgacc
    31261 ctctttgaca tcattgccca gtctatcaaa aaagacccaa gcaaggctga gaactatgct
    31321 gaaatccacc gtcgtttacg acttggaaat ctcatggtct tgacagctca aggaactcca
    31381 tttatccact ccggtcagga atatggacgt actaaacaat tccgtgaccc agcctacaag
    31441 actccagtag cagaggataa ggttccaaac aaatctcact tgttgcgtga taaggacggc
    31501 aacccatttg actatcctta cttcatccat gactcttacg attctagtga tgcagtcaac
    31561 aagtttgact ggactaaggc tacagatggt aaagcttatc ctgaaaatgt caagagccgt
    31621 gactatatga aaggtttgat tgcccttcgt caatctacag atgccttccg acttaagagt
    31681 cttcaagata tcaaagaccg tgtccacctc atcactgtcc caggccaaaa tggtgtggaa
    31741 aaagaggatg tagtgattgg ctaccaaatc actgctccaa acggcgatat ctacgcagtc
    31801 tttgtcaatg cggatgaaaa agctcgcgaa tttaatttgg gaactgcctt tgcacatcta
    31861 agaaatgcgg aagttttggc agatgaaaac caagcaggac cagtcggaat tgccaacccg
    31921 aaaggacttg aatggactga aaaaggcttg aaattgaatg cccttacagc tactgttctt
    31981 cgagtctctc aaaatggaac tagccatgag tcaactgcag aagagaaacc agactcaacc
    32041 ccttccaagc ctgaacatca aaatgaagct tctcaccctg cacatcaaga cccagctcca
    32101 gaagctagac ctgattctac taaaccagat gccaaagtag ctgatgcgga aaataaacct
    32161 agccaagcta cagctgattc acaagctgaa caaccagcac aagaagcaca agcatcatct
    32221 gtaaaagaag cggttcgaaa cgaatcggta gaaaactcta gcaaggaaaa tatacctgca
    32281 accccagata aacaagctga acttccaaat acaggaatca aaaacgaaaa caaactccta
    32341 tttgcaggaa tcagcctcct tgcgctcctt ggtctcggtt tcttactaaa aaataaaaaa
    32401 gagaactaaa ctagccctcc tatagaaaaa tcccccaagc attatagctc gggggattaa
    32461 tttttgtaca atatttgttg tcctaataaa cttgattagg attttttatt aagcctcttt
    32521 catagcaaaa taagctcgta ctttgggtgc aacttgtgtt ccgaagagtt caatagctct
    32581 cagaacctgg tcatgaggca tagaaccaag cggtagatga agcatgaagc ggtccaatcc
    32641 taaatcctct atcatgcgaa tcaatttttc ggccacctga tctggattgc caacaaacat
    32701 ggcgccattt ggccctacct gctccaaata ttgctcataa cgcaattcct gccagtgcgg
    32761 acggtctttg gaaatagcat ccaccacttg cttagtcgga tggaaataat ctttcaccgc
    32821 ctgctcacca tcttccgcaa tccaccccca agaatgggct cccactttca agtctttgtc
    32881 agcatggccc gcttcgcttc caatctcacg ataagcctga atcaactttt taaaataacg
    32941 tggattacca ccaataatag catatacaat cggtagacca gcctgagcaa tcttcactgt
    33001 tgattcgaca tgaccacctg tagctatcca caagggcaat ttgtcctgaa ctggacgagg
    33061 ataaacttct ttaccagcaa tcgtttgagt caatcgacct tgccagtcta acttggtctt
    33121 ttcattgact aactgaagca agtctaattt ctcatcaaaa agagagtcgt agtctttcaa
    33181 gtcataacca aacagaggga aagattccgt gaaagagccc cttccagcca taatctccga
    33241 tcgtccattt gacaaagcat cgatagtggc atactgttgg aacaaacgaa tcgggtccat
    33301 gcttgacaga atgctgactg cactggtcaa acggattttc ttggtattga ctgccccagc
    33361 ggccagaaca atctctgggg ctgatactgc aaaatccgcc cgatggtgct caccaatccc
    33421 atatacatcc aaaccaacct tgtcagccag ctcaatctct gccaccaact ggcgaatgcg
    33481 ttcagcatga ctgtaagttt gtccagtccc ttcaagctcc gttatttccc caaatgttga
    33541 aattcccaat tctaccattg tgattctcct tatctatctc tgtacttcaa tttgaaaaat
    33601 tattctaaca cgaatcttga gtacaagcaa ccgatttgct cattagaaaa agcctagata
    33661 actagacttt tttagcttat tctaccgtta ctgacttggc aaggttacgt ggtttgtcca
    33721 catcgaggcc acggtggagg gttgcaaagt aagcgactaa ttgcgttggt acgaccattg
    33781 aaattggtga gaggtatgga tgtacggtcg taaggacgat atcgtcggta tctttggcta
    33841 cattctcttc tgcgatagtg aggactttgg caccacgggc tgcgacctct tggatatttc
    33901 cacgagtatg attggcaaga actggatctg acaagagagc caaaacaggc gttccttctt
    33961 caatcaaggc aatggttccg tgcttgagtt ctcctgcagc aaagccttca cactggatat
    34021 aagaaatctc tttgagtttg agacttgctt ccatggctac gtagtaatct tgaccacgtc
    34081 cgatgtaaaa ggcgttacga gttgtttcaa gaagttcacg aaccttgact tcaatggttt
    34141 ctttctctga aagagttgat tcgatagact gagctacgat tgacaattca tgaaccaggt
    34201 caaaggcttg cgctttagca ttaccatttg cttctccgac tgcttttgca aggaaggcaa
    34261 gggctgcgat ttgcgctgta taggctttag ttgatgccac ggcaatttca ggacctgcgt
    34321 gaaggagcat ggtatagttg gcttcacgtg agagggttga acctggaacg tttgtcactg
    34381 ttaagcttgg aattcccatt tcattagcct tgaccaaaac ttgacgacta tccgctgttt
    34441 caccagattg gctgataaag atgaagagtg gtttcttgct gagaagtggc ataccgtagc
    34501 cccactcaga tgagattcca agttcaactg gtgtatctgt caattcttcc aacattttct
    34561 tagaagcaaa tcctgcatgg taagatgttc cagctgcaag gatgtagatg cggtctgcgt
    34621 cttgaacagc cttaatgata tctgggtcta cgacaacttg accagcctca tctgtgtagg
    34681 cttggatgag tttccgcata acagttggtt gctcgtcaat ttccttgagc atgtagtaag
    34741 ggtaagttcc cttaccgata tctgacaagt caagttcagc agtgtagcta gcacgctcac
    34801 gacgatttcc atcatagtct tgaacttcca cactatcagc cttgacgatt accaactctt
    34861 ggtcatggat ttccatgtat tggttagttt cacgaatcat agccatggcg tctgagcaga
    34921 ccatgttata gccttctcca agaccaatca aaagtggtga tttattttta gctacgtaga
    34981 tgacttcagg atcttgtgag tcaaccaagg caaaggcata agaaccacgg atgatgtgaa
    35041 gggctttttt gaaggcttca agaactgaga gcccttcttc ttccgcaaat tttccaatca
    35101 aatgaacggc tatttcagta tctgtctgcc ccttgaagtg gtgacctgca aggtattctt
    35161 ccttgatttc aagatagttc tcaatcaccc cattatgcac caagacaaaa cgttccgtct
    35221 cagagcggtg tgggtgagca ttgtcctcag ttggttttcc gtgagtagcc caacgagtat
    35281 gtccgatacc agttgttccc tcaacaccag ctgtcttggc agacaattct gcaatacgac
    35341 caaccgcctt caccaaatgg ttatcagcac catctaggac aaaaattccc gcagaatcat
    35401 agccacggta ttcaagcttt tcaagccctt gaatcaaaat atcagttgca tttgtgtttc
    35461 caacaacacc aacaattcca cacatagtat atacgacaca ggcaagctgt gctttctcct
    35521 taaaattggt atagtctaat tcatctttta tagaatcagc aaaaacagta tatacttgtt
    35581 tctttcactt gtcaagagta aaaattggta tagttcaaat taagctcctg taagcataaa
    35641 aactctgacc gattgggata atcagtcaga gtccttttta aaatccatta ttatcgctta
    35701 attctttgaa ccagtggcct gatttcttca gacgacgttc ttgcgtttcc aagtctaatt
    35761 cgaccaaacc atagcgattt ttatagctgt tgagccatga ccagcagtca ataaaggtcc
    35821 aaatcaagta gcccttacag ttggcaccat cttcaatggc acggtgaagt tcacgaagat
    35881 gaccttttac aaagtcaata cggtaatcat cttgaatcat tccatcttga cggaattttt
    35941 cttccccttc aacacccata ccattctcag tcaacatcca ctcaatattg ccataatttt
    36001 ccttgatatt ttgggcgatg tcataaatcc cttgctcata aatctcccaa ccacggtgag
    36061 aattgatttt acgtccaggc atcacataag gctcgtaaaa atgttctggt aagagtggac
    36121 tctctggatg cttagcaaat cgaggagcca taacacgcaa aggttgatag tagttcacac
    36181 caaggaagtc caccgtatta tcacgaatga gttccaactc ttcctctgta gcatcaggta
    36241 aaagaccgtg ttcatgcaag atttctacca actcctgtgg ataagtcccc aagacagatg
    36301 gatctaagaa agattgggcc tgaaaaaggg ccgcaatacg agctgccttg acatcagcag
    36361 gatgctggct acgtggataa gccggtgtca agttgaggac aatcccaatc ttggaatcag
    36421 gcaaaagttc atggcaagcc ttaacagccc ggctgctggc caattgtgta tgataggcta
    36481 ccttaacagc tgcctctgca tccaccttat gtggataatg ggcatcataa aaataaccaa
    36541 attctacagg aacgatgggc tcgttaaagg taatccattg atccactaaa tctccataag
    36601 tctcaaaaca aaaacgagca tagtcttcat aggctgagac tgtcgcctta ttttcccaac
    36661 catcaccatc ctcttgaagg gcaaaaggta aatcaaaatg atagagattg actaacagac
    36721 gaattccttt agccttaata gcctcaaaga ccttacgata aaaatccaca ccttgagtgt
    36781 tgacttttcc acagccttgt ggaaaaatcc gtgaccactg aatagaagtc cgaaaggctg
    36841 tgtgaccagt ctctaacaaa agctcaatat cccgctccca attttcataa aaagtcgatg
    36901 tcttatctga accaatccca ttatagtaac gatttggctc cacttggaac cagtaatccc
    36961 agagattgtc tcccttaccg tcaccagcta cacgtccttc tgtctgcggt ccagaagtag
    37021 aggatcccca gacaaaatcc tttggaaatc ttagcataca tttacctctt tatctactca
    37081 tttctcccat tatacagaaa aaacaaggta aaaactagtt acattttttc cttgtttttc
    37141 ttctgattat agtttttatt tcttgcttag gatttcaagc gtttcaagca cgttatctgc
    37201 atgaacctca atggtgtcac cagttgcctt gatcttaact tctacaatgc catcggccgc
    37261 ttttttacca acagtgatac ggattggaag accaatcaag tcactatcgc taaatttaac
    37321 accgacacgt tcgttacggt catctgtcaa gacttcataa ccagctccca tcaagcttgc
    37381 ttcaagtttt tctgtcaagg cttgcgcttc ttcatccttg acattgacag taatcaaatg
    37441 cacatcaaat ggtgccaatt ctttagggaa attgattccc caagcgtaac ggtattcacc
    37501 ttttggcgtt ttgttaacaa agaggcgagc gtgttgctcc atcactgctg aaagaagacg
    37561 gctgacaccg ataccgtaac atcccatgat gattggcaca gcacgaccat tttcatccaa
    37621 gacatctgct cccatgcttg ctgaatagcg agttccgagt ttgaaaatat gaccgatctc
    37681 aataccacgc gcaaagttaa ggacaccttg tccatctggg gaaatttcac cctcacgaac
    37741 ttcacggata tccacatatt ctgcagtaaa atcacggcct gggttcacac cagtcaagtg
    37801 gtagtcatct tcgttagcac cgacaactgc attgcgaaca tcttgtacct tacgatctgc
    37861 aataatttta atattctctg gcaaaccaac tggtccaagt gaaccaaatc ctgcttgaac
    37921 aacattcgcc acttcttctt cgctagcaac gtcaaagaaa tctgctccca agtgattttt
    37981 caacttgact tcgttgagtt ggtcatttcc aactagaagg gctgcaacaa gctcaccatc
    38041 tgcaatgtag aagagggttt taatcgtttg ttcttctgga acattgagga aggctgcaac
    38101 ttcatcaatt gatttaacat ctggcgttgc aacacgagta acttcttctt cagcgacaac
    38161 acggttgctt ggtttgtact cgtttgttgc catttctaag ttagctgcat agctagactc
    38221 acttgagtaa gcaatggtat cttcaccaga gactatccat ttgagcaatt ctgccttgat
    38281 ttcttcttgc acttctgcag gaatttcgtc aaatgaggca actgacttgt ccaagacaac
    38341 ccagcggtca aggtctgtac gagcagatgt aatggccata aattcttggc tatccttacc
    38401 acccatggct ccaccgtcac caataatagc cttgaagtct aaaccactac gagtgaaaat
    38461 acgctcatag gctgctttgt actcatcata aacactatcc aaactatcat agttagcgtg
    38521 gaaactataa gcatccttca tgataaactc acgtgtacga agaagtccat tacgcgggcg
    38581 tttttcatca cgatacttgg gctgaatttg ataaaggttg agtggcaatt gcttgtaaga
    38641 tttaacagaa tcacggacaa tagctgtaaa ggtttcttcg tgagttggac ctaagataaa
    38701 gtctgatttt tcacggtttt ttagtttgta aaggtcttca ccataggttt cgtaacgacc
    38761 tgattcacgc cacaattctg cactaagaag ggctggagcc aacatctcaa cagcaccaat
    38821 cttttcgaat tcttggcgca tgatgttttt agctttttca atcacacggt tggcaagtgg
    38881 tagataagaa taaacacctg ctgaaacttg gcgaacataa ccagcacgca acataagagc
    38941 atggctgata acttgagcat cgcttggcat ttcgcgaagc gttgggatag gcattttact
    39001 ttgtttcata atattcctcg attatctaaa aaagagtcgc ataatgtcat tccaagtcac
    39061 agcaatcatc aagacaacca tgatgaccac tccggccaag gtgacatagg tttcaatttc
    39121 ttgtttcaat ggtttgcggc ggatggcttc taggatattg agcacaatct taccaccatc
    39181 caaggctgga atcggaataa gattaaaaat cccaatattg atggaaatca ttgccaagaa
    39241 gtacaagata ttttcaattc catttttagc agcatcacta cttgccttaa agatagcaac
    39301 aggtccaccc aacttgttca aatctggttg gaaaatcaga tttttcagag ctgagagaat
    39361 tcggagagct gagtcagcag cagttgtaaa accacctaca aacatggata gaaaatctga
    39421 cttaaccccc ggttgaacac ctagaaggta acgaccttga ctatctttgg gtgtaacagt
    39481 gacttgtttg tcactcccct tttcagaaat agtcacatcc aaagtcggtg ccgtcttatc
    39541 tttggtttct gtttccacag cttggatcaa gctttcccag ttgctaacct catgtgagcc
    39601 aatcttggta atttgtgcca tttctggtac tcctaccttg gccaaggcac cttggggcat
    39661 gatatggaac tgattggtat caacatctct gacaccaccc tgcataaaga ttaaaaccca
    39721 aaaaacaacg acacctaaga taaaattgtt cataggacct gcaaaattgg taatcagttt
    39781 gccccagata gtcgcatttt gatattgaac atctaaaggt gcaatccgaa cctcagtacc
    39841 atctgcttcc acaaccgttg catcgtgatc cactgcaaat gttttttctt cttccagaac
    39901 caatcctttg ataaagagct tgtcttcaaa atcaaactgg gtcacctgca tagggagggc
    39961 tgtttgatcc aattttttac ctgagagatt gatgcgttta accttaccat catcagcaag
    40021 tgtcaaacta acaggcgttc ctgtcttgat ttcagttgta tcatcacccc aaccggccat
    40081 gcggacatag ccacccagag gcaagattcg aatggtatag gccgttccat ccttgccaat
    40141 gtgagcaaaa attttaggtc ccataccgat ggcaaattca cgtactaaaa tccctgattt
    40201 cttggcaaag tagaagtgac cgaactcgtg caccactaca ataatcccga aaaccagaat
    40261 aaaggttaaa attccgagca tagcgtttcc tccgtctttt gattaaaaga gtccaaataa
    40321 gtgcatgatt ggaaatacaa gcaacatact atcgaaacga tccaaaacac caccatgtcc
    40381 agggataaat ttcccagaat ccttaacacc aaaatgacgt ttgatcgaac tttctagtaa
    40441 atcaccaaat tgtccagcaa tgctaaagaa aatagcaaag actgacatct tgtaaattcc
    40501 atatggaaga gcaactgtac tgtcaactat cataaggata atggttacta aaattgctcc
    40561 taaaatacca cccaaggcac cctcaagggt tttattaggc gatacccttg gtgctaactt
    40621 tcgtttccca tagttcatcc caacaagata ggcaccactg tctgtcgccc agacgataca
    40681 caaggctaag agagccttgt ccaaacctgc aacacgagca tctagtaaag cattaaatcc
    40741 aaagcccacg tagaagctca tagcaagagg gaaaaccgca tcctcaatcg tataagactt
    40801 gctaaaaacg gtcgttccta acatgattga aatcaaaaca ctataggcaa ccacattccc
    40861 atcaactggc aaaaaagtca ggtaattctc caagggaatg gtcaatgcaa aggttgcaaa
    40921 gagggtcaag aggccctcca tcgtcatggt ctctagacct ctcatcttca aaagttcatg
    40981 catggctagc atggctatga ttccgattgc tatctgaagc aagaggcccc caatcattaa
    41041 aattggtagg aaaatagcca gggcaatccc tgcaaacaag gttcttttct gtaaatcctg
    41101 ggtcatattt cctcctaaac tcctccaaat cggcgatgac gacgattata ggcaagaata
    41161 gcttcctgca aggccgcttc gtcaaaatca ggccataagg tgtccgtaaa ataaagctca
    41221 ctataggctc cctgccatgg aaggaaattg ctcaaacgta attctccact agtacggata
    41281 atcaagtctg ggtctcgtaa gtccttaggc aaatgctgag taaagagata gttaccaatc
    41341 aattcctctg tgatgtcacc tgggttgatt ttggcatcta aaacatcctg ggaaatcaac
    41401 ttaagcgcct gtgtaatctc agcacgtcca ccatagttaa gagcaaaatt aagaatcaat
    41461 cctgtgttgt tcttagtcaa ttcctcagcc ttggttaaag cttcaaaggt ttgcttaggc
    41521 aggcggtctg tctccccaat catttgaatc ttaacattat tcgcatgtag ttccgggaca
    41581 taattatcat aaaactctac tggcaagttc atgataaact tgacttcctg atctggacgg
    41641 gtccagtttt ccgtagaaaa agcatagacc gtaataacct tgacgcccag tttgttggct
    41701 gccttggtca cggtttgcaa tgcttccatg cccgccttat gtccaaaaac tcgcggttgc
    41761 atacgttttt tagcccaacg gccattgcca tccatgatga tgccgatatg agcaggaacc
    41821 tgtgtcggaa cctctacttc cacagcctta tctttcttaa aaaatccaaa catgatctta
    41881 ttcctattca aaaatctatc gtttcattat accatatttc cccattttct tctatcacta
    41941 agctatttat tctcaggcac caagcccatt tttcaaaaaa ataagccgcc tgattgggcg
    42001 actttatttt tatagggaga ttattatgaa aaagttttag gagtttaagt taaggtcttc
    42061 ttaacttatg aacttagtgt acactcccta gcttaaagtt tccttaagta tttttaaaaa
    42121 tcaaattttt ccatttctcc tgccaatttt tcttggataa acgtgtttga tagagttcca
    42181 ttcggtcttc attttctaag aaatgaggag ttggacgaac ttgaaaattc aaaatatcct
    42241 ccaaaccata aggtacatag agttcaaaat ctaattcttc attcaagcgc agtccaactg
    42301 ccgtacaccg ttctggatac ttactcatag catcacgaga actggtatag gaagcagtgt
    42361 gaggactgtg ctgatgcata tagacctgat ttttcaattc ccactggtac tgaggaaaat
    42421 cctctctcag ctttttctcc agtaataagg tttcctcata agaaaaatct ggatcaaaga
    42481 aaatcacatc tatatctgtt tcatgatcaa aaggggattt gtctgacaaa agattccaga
    42541 tgaaatttct gacagaacct gctgccaacc acgagtcttt caaaccaagg tctcggatga
    42601 tcgtcagaat ggccatcata tctggacttt ctctaaaagc ctctaagatt tcttgcttat
    42661 ttttcactgt attcataacc taagtgctca tatgccttag cagtcgccac ccgtccagac
    42721 cgtgtccgca tgataaaacc tttttgaatc aagtaaggct catacatgtc ttcaactgtc
    42781 tcacgctctt cggcgatatt cacagaaaga gttcctagac caacaggtcc tccactgtac
    42841 atctcaatca tggtgcgaag gattttttga tccacatagt ccaaaccttc atggtcaaca
    42901 tccagcatag tcaaagcctt atcggtaata acatcatcga taaccccatt ccccattatc
    42961 tgggcaaaat cgcgcacgcg cttgaggaga cgattggcaa tacgaggggt tccacgacta
    43021 cgtagggcca actcagatgc tgcctcatgg gtgatttcca tctcaaaaat atctgccgtc
    43081 cgctcgacaa tttctgtcaa gtcagcatga gcataatact ccatatgacc tgtaatccca
    43141 aaacgtgccc gtagtggatt tgagagcata ccagcccgag tcgtcgcacc aatcaaggta
    43201 aaaggaggca actccaaatg aacactgcga ctgccttcac cagccccaat cataatatcg
    43261 atgtagaagt cctccatggc actataaagc acttcttcca ctgacatggg taagcgatga
    43321 atctcgtcaa taaagaggac atctccaggc tctaaatcat tcaaaatcgc taccaaatca
    43381 cccgcttttt cgataacagg accagacgtt tgcttgagat tgactcccag ttcattggca
    43441 atgacaaaag ccatggttgt tttcccaagc cctggagggc caaataagag cacatgatcc
    43501 agcgcttcat cccgcatttt agcggcttcg ataaagatct gaagttgatc cttaacctta
    43561 tcctgaccaa tatattcacg taaatactga ggacggagcg tgcgttctac taactcctca
    43621 tcacccatca tctcattatc taaaattcta ctcatggctc tattatatca aaaaaaacaa
    43681 gccacaaaca aaaaagccac ctgattgggt gactcctaag tttagcactt atgtggtata
    43741 atattatacg gcacttctac accgcctacg aaaggaggtg agatagccca tgatggaatt
    43801 agtactcaaa actattatcg gaccaattgt ggtcggtgtc gttcttcgta tagtcgataa
    43861 atggctaaac aaggacaaat agtgtcaaaa aagacctcaa gcttatttgg tcgtgagctt
    43921 ggggtctttt ctagcctatg atatagaact agtactcaat tcctttttat tatcccatag
    43981 ttcacgaatt ttgtcaaaac tttacatttt cttcaaccgc tgtacgacaa gacggttaag
    44041 attaagagaa cgttagggat tctatcaatt tcatagaaat tttgatttcg taaacgaaga
    44101 gacaatctta catgtcactt ctcatttaat acgccactac tagacaagca aaatcattat
    44161 tacagtagtt ccagtccttc aattaacagt cacttacaat caaattgagt ttgaactagc
    44221 tgaagcgacc acagacctat ttcttagtca tattcgctaa aaaaatcccc gccaaaatct
    44281 caaaaagtcc ccgccaattc cccgaccaaa atccgaaaaa taccgaaaaa tatcgaaaaa
    44341 ttatttttag aatagtccca aaaatcctga aatagagcta aaaaactcca cctgattcgg
    44401 tggagttaag ggagattatt atgaaaaaga aaagtttagg attttattaa ataaagttag
    44461 gaggtcttta tttaataact acatgataca agacgaaact taaaactagc ttaacttttc
    44521 taaaatttta ctattttgca aaaaatttct atcaccagca cctcaccaat cgagtagggg
    44581 ataatctcta gcccctctca caccaccgta cgtgccgttt ggcatacggc ggttcaacta
    44641 acttttaacg catgtcgttc aaggtaataa tccaaacacg aaaccagtcc acgtttttcc
    44701 aggactggtt ttgatatagc acgtttaagt accgacttct gagctactaa ttgataatgg
    44761 tcgccccagc cagatacctt atctgctatc catttaggaa ctcctaactt aagcaatccc
    44821 cataatcgtc tcgatttctt cttccattgc ttccagataa tcactcgtag gcgagtacgc
    44881 aagcgctcat ctatgctggc gactatactt ttcatatttc ccaatgagca atagtttatc
    44941 catcctcgaa tagacaaatt cagttgctca atacgtcttg ttaggtctat actccatttc
    45001 ctctgtgtta gtttcttcaa tttaaactta aatctccgaa cactatcttg atgtggacgg
    45061 cttttccaac catctgataa tttccagaac ccaaaaccta gatatttcaa ctctcttggt
    45121 catgtttact ttcaaaccta gccgtttctc aataaaacga ctgactgaat acatcacacg
    45181 cttagaggct gcctcgcttc cgaccgtaat cacacaatca tctgcgtagc gcacaaatcg
    45241 aagtcccctc ttttctaatt ccttgtccaa ttcattaagc atgatattgg ataagagagg
    45301 agataaattt cctccctgtg gtgtaccaac tagtgtttta taacgttgac cattaatgat
    45361 aacacctgaa tgaagatact tacgaatcaa ggattccgta tctccgtctt cgataatgtt
    45421 atgtactaag gacatcaatc tatcttgagg aactgtatcg aaaaatttct ctaggtctaa
    45481 tccactatcc actcatagcc gtcatttaag tattctaaga gcttcatgat ggctttttca
    45541 catgacctat ttggtctgaa accataactc gtatctgaga aatggggttc acaaatgggg
    45601 ctcatgactt ggacaatggc ctgttgaatc attctatcca taactgttgg aattcctagt
    45661 tgacggatgc ctccgtctgg tttaggtatc tcaactctaa gaactggttg aggcttatat
    45721 tttctctgtt ttatcagttc cttagtcaag cgccagtttt gtctgagata attatccatc
    45781 tcttcgatag tcattccatc aatcccagct gagcctttat tggattttac ttgattgtag
    45841 gcttccagca tattttcgcg tgataatatc ttatctagca gttttgacat gtgcgtattc
    45901 ctttcttatt ttggtgcctg agggacatct tatattggtg aaccttcatc tagtcattac
    45961 gtgtcaaagt ccagttctat cagaccactc attttacaga cacaagtgaa gtaggattac
    46021 ttcgatctgt cgttttctct ttctagcgac agacctcaaa cagtctctcc ccagactgtt
    46081 tgagtattca ccccttcgct ccacttccat tacggaagtt tcctcactac tatgggctcg
    46141 gctgacttct catgattcgt tgttactacg ctttcagcgc tcatgaaacc tcacgggata
    46201 agtcgtacat ctttcctcgt ttatacaaga tatgaggacg ttaaaaggtt acagtgaaaa
    46261 taggaaatct gacgcagagt ctttagactc tagaaagatt tatctttttc acacaaacct
    46321 tagtccgaat tcaattactc tcgtgattta cacatatagg ttacgactac ctttttggac
    46381 tttagagttt gaagcctcct tatccgctat atacgcctta ctatcacgtt cctgttcgta
    46441 gagccacgat ttcgctatcc cttcctctcc ctgctacctc acgatagtca ggctgaggac
    46501 ttacacctca gatatacgac atgcccgtcg taccgcaaaa aagccacctg attgggtggc
    46561 ttcattagga gattgtgatg aaaaaagttt aggattttat taaataaagt taggaggtct
    46621 ttatttaatg actatatgat actagaccat ccttaaactt tgcttaagaa caaacaagtt
    46681 tttttatttt tctctgttta tccaaatcat tcctgtacag tcataggtgg ataagatttc
    46741 aaagcccata gaacgataaa atcccacgtt tttttctgtc tcttctgtcg ccagctggac
    46801 ttgataggcc tctttaaaat ttcctaaagc ctctttcatc aaggagctac caatcccttg
    46861 acgctgatag ctaggcaaaa caatcaaatc ctgtacaaaa actgatgaaa aaccatctcc
    46921 aaccaaacga atcaagccca ccacagcatc accatcaagt gccagataaa ttactaatga
    46981 atgagataag gcctgctcca gcatctctgt ttgatgggta tagtttgtcc aaccgacagc
    47041 ctgatagaga tgcaaaacat cctctagctt gacaatttct tgctttttaa tagtaatcat
    47101 ctcaacacct cttaaagttc tctcaagctc ttgtgctgtc gtccattttt atcaaagttt
    47161 tcaggacgca accatatttc caatcgagct ttgacttgag gccagtcctt atcaatcata
    47221 gacaaccaat ccgtatctct tgtacgcccc ttataaacca ctgcctgacg gaaggttcct
    47281 tcataaataa atcccaaacg ttccgctgct cgtctggatg gcaggttaag agcatcgcat
    47341 ttccactcat agcgacgata gttaagctcc tcaaagacat agcaagccaa gagatactgg
    47401 gcttctgttc ctatccgtgt ccccctgagc tctggagaaa aagtgacagc tcccacttct
    47461 attactcggt tattctgatc aattcgcatg agggaaaaag ttcccaaagc cttaccagtt
    47521 gccttgtcta tgattgcata ataaaaacgg tccttacgag ccaacatctg atttaaaagg
    47581 ctgaccagtt cctccatgtc tgctactgac tcctgaaaga ggtaggtcca catctcccga
    47641 ggcgtatcag ggccataaac agctaataaa tcctccgcat gcttttccac tgaaagagcc
    47701 tctatcctag cataacgccc ttctaagaaa tcaaaagaag gcaattcacc tggagtataa
    47761 gcttccattg actccccaat catttgacca tattcattta ctggcatctt attctcctta
    47821 tttcactttt caaactttat agttgcccat agtatatcat actttcataa gaagattcaa
    47881 aaaatcctcc agattctact ctgaaggact cctatcttat ttcacaacaa tgttaactaa
    47941 tttagttggc ttcaatagtc cagtggacta ttgaaggtgg cagatagagc taataaactc
    48001 gttatttaac gacgatatta acgagtttat tcggtaccgc aattactttc acgatttcct
    48061 taccgtcaat ttctgctttg actttttcat cagctaaagc gatttcttgt aattcttcac
    48121 gtgatagatc tttagcaacc atgagtttgg cacgaacttt tcctttgatt tggacgacaa
    48181 tttcaatttc atcttcaacc aatttgcttt cgtcccaagt tggccaagct acataagaga
    48241 ttgactcacc tgtttctgcg actgtttgcc agagttcttc tgccaagtga ggtgcaaatg
    48301 gtgcaatcaa ttgaataaag cctttggcat agtcaacata aagcttatct tccttgttag
    48361 cagcattgac aaagaccata agttgggcaa tagctgtgtt gaatttgaga gactcaattt
    48421 gctcagtaac agctttgact gtttcgttgt aaaccttgtc aagagcacca ttgttttccg
    48481 caaggatttc tttacttgta atcaaacggt aaactcggtc aaggaactta cggcttcctt
    48541 ccaaaccttc ttctgaccaa gcaatcgaag catcgagtgg tcccataaac atttcataaa
    48601 cacgaagggt atcggcaccg tattgttcca ccacatcgtc tgggttaaca acgttcttga
    48661 gcgatttaga catcttggct ggcgcttgct ccaactcttc ccctgtttct acatggaaga
    48721 aggaaccatc acgtttttca accttgtcgg ttgccacaag agcaccacgg tggtcacggt
    48781 agcttgttcc caaaatcatc ccttggttaa agagtttttg gaatggttcc ttagtcggaa
    48841 caacaccgag gtcatagagg aatttatgcc agaaacgagc ataaagcaag tgaagtacag
    48901 catgttccgc accacccacg tagatatcta ctggcaacca ttgtttgagg aggtcctcat
    48961 cagccaattt ctcagtattg tgcgggtcaa tatagcggag gtagtaccag cttgaaccag
    49021 cccattgtgg catagtgttg gtttcacgac gacctttgac accatcttca cgagtcactt
    49081 caagccaatc tgtcaagtta gctagtggac tttcaccagt acctgaagga cggatatcct
    49141 tggttacagg caagacaagc ggcaattcag tttcaggaac agctgttgaa gttccatctt
    49201 cccaatgaat gattggaatt ggctcacccc agtaacgttg acggctaaag agccagtcgc
    49261 ggagacggta ggtaaccttc tcctgaccac agcctttttc ttccaaccaa gccacaatct
    49321 tggcaatagc gtcttctttg ttcaatccat ctaggaagtc tgaattgaca tgcaggccat
    49381 cctctgtgta ggcagcttct tcgacatttc caccttcaag tacttcgacg attggaaggt
    49441 caaattgttt ggcaaattcc cagtcacgtt ggtcgtgggc aggcacagcc ataaccgcac
    49501 ctgttccata actagcaagg acatagtctg caatccagat tggcatttcc ttaccattga
    49561 cagggttgat ggcataagca ccagtccaaa cccctgtttt ttctttagca aggtctgtac
    49621 gagccaagtc agacttaagg ctggcttggt gtttatagtc tgctacagct tctgcttgct
    49681 ctgaacttgt gatagcgtct actaattcat gttcaggagc caagacagtg aaagtcgcac
    49741 cgaaaagtgt gtccggacga gtagtaaaga ctgtaaattc cttgtctgtt ccttttactt
    49801 tgaaagttac attggcacca gttgatttac caatccagtt gcgttgcata tccttgatag
    49861 actctgacca atctagttca tctaagtcat tgagcaagcg ctctgcgtaa gccgtgattt
    49921 tgagcatcca ttggcgcatt ggtttgcgga caactggata gcctccacgc tcagaagttc
    49981 cgtcaggaag cacttcttca ttggcaatgg cagttcccaa ttcctcaacc cagtttactg
    50041 gcacttcagc ttcataggcc aagccttttt cgtaaagctt ggtgaaaatc cattgagtcc
    50101 acttgtagta gtttggatct gttgtgttga cttcacgatc ccagtcataa gaaaatccaa
    50161 gcgcattaat ttgacgtttg aagttggcaa tgttttccgc tgtaaattct gctgggtcat
    50221 taccagtatc catagcgtat tgctctgcag gcaaaccaaa agcatcccaa cccattgggt
    50281 gaaggacatt gtagccttgc gcacgtttgt aacgactgag gatatcggtt gcagtataac
    50341 cttctgggtg tcctacgtgc agaccagctc cagacggata agggaacata tcaagcgcat
    50401 aaaacttagg ttttgatgta tctgttcctg tcttaaatgt atgatgttct gcccagtagc
    50461 cctgccactt aggctcaatt tctttatgat tgtaaaaact catggtctct ctccaatttt
    50521 gtgatgttac tattatacca tttttgaggg aatttgaaat gtgtttaaag gaaaaactgt
    50581 atactgtatc tcattaattt tgatgactat gaattgctct atctcccact ttttatccca
    50641 ttgttatgca gtaaatcaag gagtaagtca agaagcttat ctcgtatagc acaaaatact
    50701 tattttagac cgaagataaa actggaatgc taattaatgt attcacaata aatagccgac
    50761 aacctatgtc gctttttgat ttatgaagaa catatagtag actgaatctg aaatagtacg
    50821 aaacaattgc taaaacattt atagaaatta attttacttt cccaatcgat ttgttctcat
    50881 cttatttcaa tctg
//
LOCUS       DAVKNL010000007        50846 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVKNL010000007 DAVKNL010000000
VERSION     DAVKNL010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 50846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50846
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.007"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            468..1511
                     /locus_tag="VW411_000361"
     CDS             468..1511
                     /locus_tag="VW411_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEU9793159.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            1678..3765
                     /gene="topA"
                     /locus_tag="VW411_000362"
     CDS             1678..3765
                     /gene="topA"
                     /locus_tag="VW411_000362"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEU9793160.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNNEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            3986..4345
                     /locus_tag="VW411_000363"
     CDS             3986..4345
                     /locus_tag="VW411_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEU9793161.1"
                     /translation="MRIIYLIIGFLSLTLAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQAYVADFRETKSIARERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            4438..5070
                     /locus_tag="VW411_000364"
     CDS             4438..5070
                     /locus_tag="VW411_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEU9793162.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQAEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLENRQTFIDQVGVTQLHGTKVVF"
     gene            5114..5770
                     /locus_tag="VW411_000365"
     CDS             5114..5770
                     /locus_tag="VW411_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEU9793163.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVYMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            5789..6883
                     /locus_tag="VW411_000366"
     CDS             5789..6883
                     /locus_tag="VW411_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEU9793164.1"
                     /translation="MNFQLAKYSLLKRFLENIGFTTLEEYGAIFKYLIENVKTDRQII
                     YSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAENAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKILLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALVDKKQEITDADI
                     RALVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEFNATGQGSVEAIF
                     NAIDKFFNQSVRLVSYTIDAVTDGIDTQDRVLVTVENRDTETIFNAAGLDFDVLKASA
                     IVYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            6895..7932
                     /gene="leuB"
                     /locus_tag="VW411_000367"
                     /pseudo
     CDS             6895..7932
                     /gene="leuB"
                     /locus_tag="VW411_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
     gene            7929..8198
                     /locus_tag="VW411_000368"
     CDS             7929..8198
                     /locus_tag="VW411_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEU9793165.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKSKARRRVWRIPEKILLIL
                     AFTFGGFGAWLAGIIFHHKTRKWYFKIVWFLGMVTTLVALYFIWR"
     gene            8202..8561
                     /gene="leuD"
                     /locus_tag="VW411_000369"
     CDS             8202..8561
                     /gene="leuD"
                     /locus_tag="VW411_000369"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEU9793166.1"
                     /translation="MAGSSREYAAWALADYGFKVVIAGSFGDIHYNNELNNGMLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            8780..8923
                     /locus_tag="VW411_000370"
     CDS             8780..8923
                     /locus_tag="VW411_000370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793167.1"
                     /translation="MTSDKAGLERKFAAKERKRNKPGVVLCGSMDELCALAQLNPEIE
                     AFY"
     gene            9184..9345
                     /locus_tag="VW411_000371"
     CDS             9184..9345
                     /locus_tag="VW411_000371"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793168.1"
                     /translation="MVDKDGKLIPEQGGARSTSPAPVVIRKGLDIDKIMMHLSDTFNS
                     WDYRQVEYY"
     gene            9509..11440
                     /locus_tag="VW411_000372"
     CDS             9509..11440
                     /locus_tag="VW411_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HEU9793169.1"
                     /translation="MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEW
                     PLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNK
                     YKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
                     LLCIYTEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKL
                     GTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEI
                     AKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKE
                     TQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLP
                     SKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKNFVFIIDEI
                     NRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDR
                     SVDTFDFAMRRRFRFVEVTVEGQAGMLDKELNIHAEEAKIRLRNLNAAIENIQELNSH
                     YHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFDLTNNEQ
                     KDQAVADDNEGDENDDADY"
     gene            11427..12743
                     /locus_tag="VW411_000373"
     CDS             11427..12743
                     /locus_tag="VW411_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEU9793170.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNPLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLVYLFPKYLQAAIRKGLYKEYHRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYATREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLIFPSMEQSVNSEIGKV
                     AGYGAQLKKWSIRIPQNASFYSTFCKMMENSEENFKAIIDEEVGRK"
     gene            12918..13904
                     /locus_tag="VW411_000374"
     CDS             12918..13904
                     /locus_tag="VW411_000374"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEU9793171.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHNQGLIASISVGVKDYEY
                     DFVSQLKADTPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            13968..14039
                     /locus_tag="VW411_000375"
     tRNA            13968..14039
                     /locus_tag="VW411_000375"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14001..14003,aa:Arg,seq:ccg)
     gene            14222..14920
                     /locus_tag="VW411_000376"
     CDS             14222..14920
                     /locus_tag="VW411_000376"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEU9793172.1"
                     /translation="MKELQTVLKNHFAIEFADKKLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            14911..18450
                     /gene="smc"
                     /locus_tag="VW411_000377"
     CDS             14911..18450
                     /gene="smc"
                     /locus_tag="VW411_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEU9793173.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDIIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HVYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELTQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKSDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDNLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDVYYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDAIAVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAEEEASLGSEEATLKTLQDEMARLTESLEAIKSQGEQARIQEQGLFLAYQQTSQQV
                     EELETLWKLQEEEIDRLSEGDWQADKENCQERLAAISSDKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLDFEQEEIQRLLQEKIDN
                     LEKVDTELLSQQAEESKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQSQLTDQYQISYTEALEKAHELENLNLAEQEVKDLEK
                     AIRSLGPVNLDAIEQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEVIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            18447..19241
                     /locus_tag="VW411_000378"
     CDS             18447..19241
                     /locus_tag="VW411_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9793174.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLVSYKEKGIYFAVAS
                     GRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEDGEAWVNENVPGVKAMTTGFESIDIVLDYVDKGVAIVELVKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKERSVIAYMEGL"
     gene            19241..20059
                     /locus_tag="VW411_000379"
     CDS             19241..20059
                     /locus_tag="VW411_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9793175.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLQAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            20063..21352
                     /gene="ftsY"
                     /locus_tag="VW411_000380"
     CDS             20063..21352
                     /gene="ftsY"
                     /locus_tag="VW411_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEU9793176.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAEVEIVEQAVFQEEEIQDTVEESQDSEPAVGVSQEEIEELPNSEELPEEEKLEHE
                     GTVEENNSEVLEPEAPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEE
                     LEELLIMSDVGVQVASNLTEELRYEAKLENAKKPDVLRRVIIEKLVELYEKDGSYDES
                     IHFQDNLTVMLFVGVNGVGKTTSIGKLAHRYKQAGKKVMLVAADTFRAGAVAQLAEWG
                     RRVDVPVVTGPEKADPASVVFDGMERAVTEGIDILMIDTAGRLQNKDNLMAELEKIGR
                     IIKRVVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAI
                     REELNIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(21398..22885)
                     /locus_tag="VW411_000381"
     CDS             complement(21398..22885)
                     /locus_tag="VW411_000381"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU9793177.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYQSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVCASWKLIDRIEKLWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDITYRQDGRLE"
     gene            complement(23034..23774)
                     /locus_tag="VW411_000382"
     CDS             complement(23034..23774)
                     /locus_tag="VW411_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9793178.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(23774..25939)
                     /locus_tag="VW411_000383"
     CDS             complement(23774..25939)
                     /locus_tag="VW411_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU9793179.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALSEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            26109..27062
                     /locus_tag="VW411_000384"
     CDS             26109..27062
                     /locus_tag="VW411_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9793180.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            27139..29127
                     /gene="uvrB"
                     /locus_tag="VW411_000385"
     CDS             27139..29127
                     /gene="uvrB"
                     /locus_tag="VW411_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEU9793181.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTIGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            29137..29700
                     /locus_tag="VW411_000386"
     CDS             29137..29700
                     /locus_tag="VW411_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9793182.1"
                     /translation="MIYLRKLKKEDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKETRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNIGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            29666..30154
                     /locus_tag="VW411_000387"
     CDS             29666..30154
                     /locus_tag="VW411_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9793183.1"
                     /translation="MTVIIKSMETPEEIEGKSFVHWQTWREAYDDLLPAEFQETMTLE
                     RCRLFSQKYPENTLIAMDGVKIVGFISYGNCRDETIQAGEIIALYVLKDYYGKGIAQK
                     LVKAALTDLNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SK"
     gene            30223..30690
                     /locus_tag="VW411_000388"
     CDS             30223..30690
                     /locus_tag="VW411_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9793184.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGTLQS
                     SEEGEVSWVQKDQIPNLNLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
     gene            complement(30687..31202)
                     /locus_tag="VW411_000389"
     CDS             complement(30687..31202)
                     /locus_tag="VW411_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEU9793185.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQRLAEHFHVTRQVIVQDIAILR
                     ADGSPILSTNRGYIYKDANANTYHHKLFKVKHEVEEIGRELLAIVDNGGRVQNILIDH
                     PVYGEIETLLKLTCRRDVQHFLEQVERSDFKPISELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(31214..31741)
                     /locus_tag="VW411_000390"
     CDS             complement(31214..31741)
                     /locus_tag="VW411_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9793186.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWIFNFVLAVVHALAEVLACVVFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            complement(32023..32586)
                     /locus_tag="VW411_000391"
     CDS             complement(32023..32586)
                     /locus_tag="VW411_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9793187.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            complement(32570..33121)
                     /gene="coaC"
                     /locus_tag="VW411_000392"
     CDS             complement(32570..33121)
                     /gene="coaC"
                     /locus_tag="VW411_000392"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEU9793188.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            complement(33133..33822)
                     /gene="coaB"
                     /locus_tag="VW411_000393"
     CDS             complement(33133..33822)
                     /gene="coaB"
                     /locus_tag="VW411_000393"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEU9793189.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGYEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISADQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            34052..35722
                     /locus_tag="VW411_000394"
     CDS             34052..35722
                     /locus_tag="VW411_000394"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEU9793190.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRVVDLNDRTLRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAAL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            36050..37225
                     /gene="mutY"
                     /locus_tag="VW411_000395"
     CDS             36050..37225
                     /gene="mutY"
                     /locus_tag="VW411_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEU9793191.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQVYAQANLDSSQD"
     gene            37281..37985
                     /locus_tag="VW411_000396"
     CDS             37281..37985
                     /locus_tag="VW411_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9793192.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            37978..39327
                     /gene="vicK"
                     /locus_tag="VW411_000397"
     CDS             37978..39327
                     /gene="vicK"
                     /locus_tag="VW411_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEU9793193.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQV
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            39329..40138
                     /locus_tag="VW411_000398"
     CDS             39329..40138
                     /locus_tag="VW411_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU9793194.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            40229..40471
                     /locus_tag="VW411_000399"
     CDS             40229..40471
                     /locus_tag="VW411_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEU9793195.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            40473..40727
                     /locus_tag="VW411_000400"
     CDS             40473..40727
                     /locus_tag="VW411_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU9793196.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            40802..42674
                     /locus_tag="VW411_000401"
                     /pseudo
     CDS             40802..42674
                     /locus_tag="VW411_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
     gene            42839..46098
                     /locus_tag="VW411_000402"
                     /pseudo
     CDS             42839..46098
                     /locus_tag="VW411_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143847.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            complement(46408..47394)
                     /locus_tag="VW411_000403"
     CDS             complement(46408..47394)
                     /locus_tag="VW411_000403"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEU9793197.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            47588..50056
                     /gene="gyrA"
                     /locus_tag="VW411_000404"
     CDS             47588..50056
                     /gene="gyrA"
                     /locus_tag="VW411_000404"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU9793198.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMIGQVLSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            50056..50799
                     /gene="srtA"
                     /locus_tag="VW411_000405"
     CDS             50056..50799
                     /gene="srtA"
                     /locus_tag="VW411_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEU9793199.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSNEILTAFNQPYKQFY"
BASE COUNT        15869 a         8889 c        11220 g        14868 t
ORIGIN      
        1 acactataat ttagtgtaat tgagaaagga gaaatgattg tgattgatgt tggctaggtt
       61 atgttcaatg attcctaccg tctcaaatct tgtcagtaag gaaaaataaa ttcttcaaaa
      121 gtagagatta caaggcttgt ttaagaaaga attcaaagac cttgacaaat aaaaataaaa
      181 tggttattat aaaaaatggt ctgaaataga tgatgatact tttcgaaaat ctcttcaaac
      241 tacgtcagct cagctttgcc ttgctgtgtt ttgagcaagc tacggttagc ttccgagttt
      301 gattttcatt tactagaaat gaaactgatg agagatatca gtagacattt gagtcaggat
      361 attatggaaa atgataaaaa gagctcgtga gattggcata tcagactact aaagtattga
      421 gtttgttagg attttagcga ctagttagct gggaaaggaa gatatttgtg acaaataata
      481 aactgtattc gttgatagaa tttagaaata aaatatatga agaattagaa ctttccagaa
      541 gtgatttagc gattttacta tgtgccatgc ttatcgcctc tatcggatta aatatggatt
      601 cgactcccgt gattattgga gccatgttaa tctctccttt gatgacacct attctgggag
      661 tggggctctc tctagctata tttgatttta aattgttaag aaaatctttt aaaatattag
      721 ctattcaaat tcttgccagt ctaatagctt caacacttta tttttatctt tctcccattt
      781 cgtatgctag ttcggagatt gttgctagaa cctctccgac tatttgggat gttctcattg
      841 cttttgtagg agggatagca ggtatcattg gtgctaggaa aaaagagacc aataatattg
      901 ttcctggtgt tgctattgca accgccttga tgcctcctct ttgtacagta ggttatgcta
      961 ttgcttctgc taatctaaaa tttatcatag gctcctctta cctattcctc atcaattgta
     1021 gctttattgt cattgcgact tatataggtg ttaggttgat gatggttaag aaacattatt
     1081 ttaaagataa tgaagaagac tctaaaatgc gtaggatttt gcttctagtt gctgttttgc
     1141 tgatgattcc gagtttcatc tctgcaacga ctttagtgag agaaacgttg aaaaaagagt
     1201 cccttaagaa atttatatca gagcagtttc aggggtataa tattttgaaa aaaacctatt
     1261 ctaaaaagac tcatacccta aagctaacca tttcaggaaa ttatttgaca gaagaagaac
     1321 tcgatatgat ttcaagtaag agaggtgact atggtttaag tgatgtttct gttcaagttt
     1381 cacaattgtc tgattcagaa caacttagta aggaagagct ggtggagtat ttcttccagt
     1441 atatcaagga taaggaagca aaagaaaagg agaaagccaa taaatttgat acagagtctg
     1501 aggagcaata atttcttgag aatagctggt ttctctcgtg agtcttctat gtatatcaaa
     1561 ggaagactga ggttttaagt ataaaacttt tcttctatta tagtagctaa gtcttgacag
     1621 ggttcaaaaa atggtttaca ctttataaag tttattactt tgaaaaggtg tgatactgtg
     1681 gctacggcaa caaaaaagaa aaaatcaaca gttaaaaaaa atctagtcat cgtggagtcg
     1741 cctgctaagg ccaagacgat tgaaaaatat ctaggcagaa actacaaggt tttagccagt
     1801 gtcgggcata tccgtgattt gaagaaatcc agtatgtccg tcgatattga aaataattat
     1861 gaaccgcaat atattaatat ccgaggaaaa ggccctctta tcaatgactt gaaaaaagaa
     1921 gctaaaaaag ctaataaagt ttttctcgcg agtgacccgg accgtgaagg agaagcgatt
     1981 tcttggcatt tggcccatat tctcaacttg gatgaaaatg atgccaaccg tgtggtcttc
     2041 aatgaaatca ccaaggatgc agtcaaaaat gcttttaaag aacctcgtaa gatcgatatg
     2101 gacttggtcg atgcccaaca agctcgtcgg atcttggatc gcttggtagg gtattcgatt
     2161 tcgcctattt tgtggaagaa ggtcaagaag ggcttgtcag caggtcgcgt tcagtccatt
     2221 gcccttaaac tcatcattga ccgtgaaaat gaaatcaatg ccttccagcc agaagaatac
     2281 tggacagttg atgctgtctt taaaaaggga accaaacaat ttcatgcttc cttctatgga
     2341 gtagatggta aaaagatgaa actgaccagc aataacgaag tcaaggaagt cttgtctcgt
     2401 ctgacgagta aagacttttc agtagatcag gtggataaga aagagcgcaa gcgcaatgct
     2461 cctttaccct ataccacttc atctatgcag atggatgctg ccaataaaat caatttccgt
     2521 actcgaaaaa ccatgatggt tgcccaacag ctctatgaag gaattaatat cggttctggt
     2581 gttcaaggtt tgattaccta tatgcgtacc gattcgactc gtatcagtcc tgtagcgcaa
     2641 aatgaggcgg caagcttcat tacggatcgt tttggtagca agtattctaa gcacggtagc
     2701 aaggtcaaaa acgcatcagg tgctcaggat gcccatgagg ctattcgtcc gtcaagtgtc
     2761 tttaatacac cagaaagcat cgctaagtat ctggacaagg atcagcttaa gctatatacc
     2821 cttatctgga atcgttttgt ggctagccag atgacagcgg ccgtttttga taccatggct
     2881 gttaaattgt ctcaaaaagg ggttcaattt gctgccaatg gtagtcaggt taagtttgat
     2941 ggttatcttg ccatttataa tgattctgac aagaataaga tgttaccgga catggttgtt
     3001 ggagatgtgg tcaaacaggt caatagcaaa ccagagcaac atttcaccca accgcctgcc
     3061 cgttattctg aagcaacact gattaaaacc ttagaggaaa atggggttgg acgtccatca
     3121 acctacgcgc caaccattga aaccattcag aaacgttatt atgttcgcct ggcagccaaa
     3181 cgttttgaac cgacagagtt gggagaaatt gtcaataagc tcatcgttga atatttccca
     3241 gatatcgtaa acgtgacctt cacagctgaa atggaaggta aactggatga tgtcgaagtt
     3301 ggaaaagagc agtggcgacg ggtcattgat gccttttaca aaccattctc taaagaagtt
     3361 gccaaggctg aagaagaaat ggaaaaaatc cagattaagg atgaaccagc tggatttgac
     3421 tgtgaagtgt gtggcagtcc aatggtcatt aaacttggtc gttttggtaa attctacgct
     3481 tgtagcaatt tcccagattg ccgtcatacc caagcaatcg tgaaagagat tggtgttgag
     3541 tgtccaagct gtcatcaggg acaaattatt gagcgaaaaa ccaagcgtaa tcgcctattc
     3601 tatggttgca atcgctatcc agaatgtgaa tttacctctt gggacaagcc tgttggtcgt
     3661 gactgtccaa aatgtggcaa cttcctcatg gagaaaaaag tccgtggtgg tggcaagcag
     3721 gttgtttgta gcaaaggcga ctacgaggaa gaaaagatta aataagagga ggggcccgaa
     3781 agtaaatttc aggctccttt tggttagaac ttgacaaaaa acatcatttt atgcgaaact
     3841 agaagaagat tattttatac tcaatgagaa tcaaagagca aattagaaag ctagtcgcag
     3901 gttgctcaaa gcactacttt gaggttgaag atggaagctg acctggtttg aagagatttt
     3961 cgaagagtat taactaggag aagttatgcg tattatctat ctaattattg gttttttatc
     4021 gctgaccttg gctattgttg gggttgtttt acccttgttg cctacaacac ctttcctttt
     4081 gttgtctatt gcttgtttct ccagaagttc caagcgattc gaagattggc tttatcatac
     4141 caagctctat caagcatatg tagctgattt tcgtgagacc aagtctattg cgcgtgaacg
     4201 aaagaaaaaa atcatcgtct ctatctacgt cttgatggga atttctattt attttgcacc
     4261 tcttttacca gtcaaaatcg gtctgggtgc tttgaccatc tttattactt attatctctt
     4321 caaggtcatt ccagacaaag aatagttaaa acagtagtta tttgccttga taaaattgaa
     4381 agcatattca taacaatatg atataataaa attgaagtaa tattcaagga gaatcaaatg
     4441 atttacgaat tttgtgctga aaatgtgact ttacttgaaa aagcgatgca ggctggagct
     4501 cgtcggattg aactctgtga taatctagca gttggtggga caacacccag ctatggagtg
     4561 actaaggcag cggttgaact ggcagctaac tacgatacaa ccatcatgac catgattcgg
     4621 ccacgtggtg gtgactttgt ctataatgac ctagaaattg ctatcatgct agaagacatt
     4681 cgtttgactg ctcaggctgg aagtcaaggg gttgtatttg gagctttaac tgctgataaa
     4741 aagttggata agcctaatct ggaaaagtta attgctgcat caaaaggaat ggaaattgtc
     4801 tttcacatgg cctttgatga actaagtgat gaagatcaag cggaagctat tgactggctc
     4861 agtcaagccg gtgtcactcg tatcctaact cgtgctggtg tgtctggcga ctccttagaa
     4921 aaacgttttg ttcactatca cagaattttg gagtacgcta aaggtaaaat tgaaattcta
     4981 ccaggtgggg ggattgacct tgaaaaccgt caaaccttta tcgaccaggt gggggtaaca
     5041 caattgcatg gtactaaggt tgttttttaa aaaatagaaa ggaactgcta gctttgggta
     5101 gcagttttca cttatgtttg aaatttttaa atcctatcaa tttaatcaag aaaaggctca
     5161 tgattatggt tttatagaaa atagcgaagt ctggacatat agttgccaga ttttgcaagg
     5221 tgactttgtc atgactgtgt ccatcactgc tgataatgtg aactttcaag tctttgacca
     5281 agagactggt gacctctatc ctcacgttta tatggaaagc atgaggggaa gttttgtcgg
     5341 aaatgtccgt gaggcttgtc tggagattct ttaccagatt cggaaggctt gttttgatgt
     5401 gcaagatttt atctgtcatc agactaagcg tatcatgact caagttcagg aaaagtatgg
     5461 aaaccagttg gagtatctgt gggaaaaatc gcctgataca gctgtattgc gccatgaagg
     5521 caatcaaaag tggtatgccg tcttgatgaa aatctcttgg aataagctgg aaaagggcag
     5581 agaaggacaa gtggaagcag tcaacctcaa gcatgaccaa gtagctaatt tgctttcaca
     5641 aaaggggatt tatccagcct tccatatgag caagcgctac tggattagtg tgtcccttga
     5701 tgatacttta tcagatgaag aagtactgga attgatagaa aaaagttgga acttaacctc
     5761 taaaaaatga aatattttaa taattttcat gaactttcaa ttagctaaat attctttact
     5821 gaagagattt ttagaaaata taggatttac cacactagag gaatatggtg ccatcttcaa
     5881 atacctgatt gagaatgtca agacggatcg tcagatcatc tattcgcctc actgtcatga
     5941 tgacctcgga atggcagtgg caaatagcct tgctgctgtc aagaatggtg caggacgtgt
     6001 tgaagggact atcaatggta ttagggagcg agctgaaaat gctgctttgg aagaaattgc
     6061 agtggctctc aatattcgcc aagattacta ccaagtagaa accagtattg tcctaaatga
     6121 gaccatcaat acgtcagaaa tggtttctcg cttctctggt attccagttc ctaaaaacaa
     6181 agccgtcgtt ggtggcaata ccttctccca cgaatctggt attcaccaag atggagtcct
     6241 taaaaatcct ctcacttatg agatcatcac acctgaattg gttggtgtta agattctgct
     6301 tggaaaatta tctggtcgcc atgcttttgt tgagaaactg agagaattgg ccctagattt
     6361 tacagaagag gatatcaaac cactctttgc taagttcaag gcactggtcg ataagaagca
     6421 agaaatcaca gatgcagata ttcgagcttt ggtagctgga accatggttg aaaatccaga
     6481 aggcttccac tttgatgatt tacaacttca aactcatgca gataatgaca ttgaagcgct
     6541 cgttagccta gccaatatgg atggtgagaa agtcgaattt aatgcgacag ggcaaggttc
     6601 cgttgaagca atctttaatg ctatcgataa gttctttaac caatctgttc gtttggtgtc
     6661 ctacactatc gatgcggtaa cagatggaat cgatacccag gatcgggttt tggtcactgt
     6721 tgaaaacaga gatacagaaa ccatctttaa tgcagcaggg cttgattttg atgtgttgaa
     6781 ggcttctgct attgtctata taaacgctaa tacctttgtt caaaaagaga atgcaggtga
     6841 gatgggacgc agtgtttctt accacgatat gcctagtgtg taaaggagaa ggctatggca
     6901 aagaaaatag tagctctagc aggagacgga attggcccag aaatcatgga ggttggttta
     6961 gaagttctgg aggctctagc tgaaaaaaca ggttttgact atgagattga cagacgaccg
     7021 ttcggaggtg cagatattga tgcagcatga cctcccttac ctgatgaaac ccttaaggca
     7081 agtagggaag cagatgctat cctactagta gctatcggta gtcctcagta tgatggagca
     7141 gtggttcgcc ctgaacaagg cctgatggct ctccgtaagg aactcaatct ttacgctaat
     7201 attcgtcctg taaaaatctt tgacagtctc aagcatttgt caccactcaa actggaacga
     7261 attgctggtg tagactttgt cgtggtgcgt gaattgacag gcgggattta ctttggatat
     7321 catattcttg aagagcgcaa tgcgcgtgat atcaacgact atagctatga ggaagtggag
     7381 cggattattc gcaaagcctt tgaaattgca agaaatcgca gaaaaatcgt tactagtatc
     7441 gataagcaaa atgttctagc gacctcaaaa ctctggcgga aagtagctga ggaagtcgca
     7501 caggatttcc cagatgtaac cttggaacat cagctggtag actcagctgc tatgcttatg
     7561 attaccaatc ctgctaagtt tgatgttatt gtaacggaga atctttttgg agatatttta
     7621 tctgatgaat caagcgtctt atctggtaca cttggggtta tgccatcagc cagtcattct
     7681 gaaaatggac caagtctcta tgaacctatt cacggttcag cacctgatat tgcaggtcaa
     7741 ggaattgcca atcctatttc catgatttta tcagtttcca tgatgttgag agatagtttc
     7801 ggacgttatg aggatgcaga gcgtatcaaa cgtgctgttg agacaagtct ggcggcagga
     7861 attttaacga gagatatagg aggtcaggct tcaacaaagg aaatgacgga agctattatt
     7921 gcaaggttat gaagttagac gaaaaaatta ctctagtcct tttgatttgg aatgtcatca
     7981 ttttcttgat ttatggtatt gacaaatcta aggcaaggag aagagtttgg cgcatccctg
     8041 agaaaatctt acttatttta gcctttactt ttggtggttt tggtgcctgg ctagcaggaa
     8101 tcatctttca ccacaagact cgaaaatggt actttaaaat agtttggttt cttgggatgg
     8161 tgaccacact agtagcctta tattttattt ggaggtaatg gatggcaggg tcttcgaggg
     8221 aatacgctgc ttgggctcta gcggactatg gttttaaggt cgtgattgca ggatctttcg
     8281 gtgacattca ttacaataat gaactcaata atggcatgtt gccaatcgtt cagcctagag
     8341 aggttagaga gaaactagcc cagctaaaac caaccgacca ggtaactgtg gacttggaac
     8401 aacaaaaaat catctcacca gttgaagaat tcaccttcga gatagatagc gagtggaaac
     8461 ataaactcct aaatagtttg gatgatatcg gtattacctt gcagtatgaa gagttgattg
     8521 ctgcttatga aaaacaacga ccagcctact ggcaggatta gaaaaaatag aaaaggagat
     8581 atagtaaact gaaataagat gtaaacaaat gaattggagc ttaacatcca tttccagcaa
     8641 ttttttagaa actacagtgg actattctgg attcaacaca ttataaaatt atgacaaaac
     8701 acattcacaa gaaggctacg acattttaaa aggtgagggc ggatgtatcg tttgccctac
     8761 taaagttggt tacattatca tgaccagtga caaggcagga cttgagcgta agttcgcagc
     8821 caaagaacgt aagcgtaaca aaccaggtgt tgttctctgc ggtagcatgg atgaactttg
     8881 cgctttagcg caactcaacc cagaaattga agcattctac taaaaacatt gggatgaaga
     8941 tattcttctt ggttgtatcc ttccttggaa accagaagcc tttgaaaaac tcaaagcata
     9001 cggggatggc cgtgaagaac ttattactga tgtacgtggt actagctgtt ttgttatcaa
     9061 gtttggaaaa gcaggtgaac aattggctgc caagctttgg gaagaaggta aaatggtcta
     9121 cgcctcatct gcttcaatga caaaacgatt gaaactcgct atgagcaagg tgtaatggtg
     9181 tctatggtcg ataaggacgg caaactcatc ccagaacaag gaggagcacg ttcaacttca
     9241 ccagctccag ttgtgatccg taaagggctt gacattgata aaatcatgat gcacctgtca
     9301 gatactttta actcatggga ctaccgtcag gttgagtatt attaggatag agaagaagtc
     9361 tagtgttatg agatattaaa gctcctaaca ctgggctttt gtttagaatt tcttttcttt
     9421 ttctatagga tatggtattc tatgtagaaa atatatgtta ataagtaatg ccaatattta
     9481 aacatcatta gtaaaaggag ttagattgat gaataaaaga aaagttagtt tagaagattt
     9541 ttataaatgg tatagtctaa ataaagaaga gttattaaat aaggcaactg ttggtgaaaa
     9601 gtttaatgat aaattaaaag aagagtttct ccaggaatgg cctttggata ggattttaac
     9661 aatgtcaatc gatgaatatg taataggaaa gggacagcaa aataagtctt tatgctacgc
     9721 tcttgagaag ggaaaataca aaaatctatt tcttggaatt tctggtggct cagcttcaaa
     9781 atttggtatt tattggaata aaaaaacaaa caaatataaa gatcaagcta ataatgagat
     9841 ttcagagttg gatcagcgat tttcaaaatt aaaatcagat ttgtatgaaa ttatcaaaga
     9901 aggtattcgt tttaactttg aaaatcctat ttttgatatg aaaagatcaa caaatgaatt
     9961 tattggtcgt tctgctatgg tgacaaaatt actttgtatc tatactgagg gagatccttt
    10021 ctttggtgta aatattaata gtcagaaaga attttggaac cactttgttt ctcagacaaa
    10081 tcaaggtgga ccttatctgc aaaatcataa aataattgaa ctggtgtcca aaacttatcc
    10141 tgagttggag ccatcgaaat taggaactat gctttttgag tattctaagc tttttatgga
    10201 aaataaggaa gacaatagta caatggattc atcaaacaat tttcgtcatc aattaactca
    10261 atctctatta aagtctccaa acctcatcct ccgcggtgct cctggcacgg gaaaaactta
    10321 tcttgctaaa gaaattgcta aagaattaac ggatggcaac gaagatcaaa tcggatttgt
    10381 acaatttcac ccatcatatg attatacgga ttttgtagaa ggtttaagac cagtatcaaa
    10441 tggggatgga gctattgagt ttaggctaca ggacggtatt tttaaagatt tttgtcagaa
    10501 agcaaaagaa acccaattga ttggaggaca agataatttt gatgaggctt gggattctta
    10561 cttagaatat ataaatgttg ctgaagaaaa agaatatata acaaaaacat cttacttatc
    10621 tgttaatagt agacaaaatt tgtcagtaaa ttatgatagt ggtgttccag gatggtcact
    10681 acctagcaaa tatgtttacg agttgtataa agataaaaat tataataagc aagaatacta
    10741 caaaagtggt ggaaaaactg tcctagaaac attgagaaag agatttggtt tgaaagacta
    10801 tgtttcccca acagaaattg atactgataa gaattttgtc ttcatcatcg atgagatcaa
    10861 tcgtggggag atttctaaga tttttggcga actctttttc tctatcgacc ccggctatcg
    10921 tggtgaaaaa ggaagtgttt ctacccaata tgcaaatcta cacgaaactg atgaaaagtt
    10981 ctatatcccc gaaaatgttt acatcatcgg aactatgaat gatattgatc gttcagtgga
    11041 tacctttgat tttgctatgc gtcgtcgttt tcgttttgtt gaagttactg tcgagggtca
    11101 agctggcatg ttggataaag agttgaatat ccatgcagaa gaagcaaaaa ttcgtctaag
    11161 aaacttgaac gctgctatcg aaaatattca ggaattaaac agtcattatc atattggacc
    11221 aagttatttt cttaagttga aggatgtaga ttttgactat gaattactct ggtctgatta
    11281 tattaagcct ctcctagaag actacttgcg aggttcttat gatgaggttg aaactttgga
    11341 aactttgaaa aaagcatttg atctgacaaa taatgagcaa aaagatcagg cagtagctga
    11401 tgacaatgaa ggcgatgaaa acgatgatgc ggattactga taatcaacac aagattatta
    11461 aagaaaaatt tgttgaagaa tatcctaaac taagcaatcc tcttttagac agaaccttgg
    11521 aaagtctatc ccaagatgaa cgtattttca tttttccaaa tgatttgact catactcctg
    11581 atttggataa ggaccaaaag atttttgaaa cagtcaatca gaaaatcaag acagggaacg
    11641 tgattggttt tcttggatat ggtcaggaaa gattaacgat ttcctcacga ttttctgatg
    11701 agagtaatga ccactttttg cattatctct taaacaaggt tcttcatatc aatctcacta
    11761 gtttagatgt tgctttgtct cgtgaagaga ggctttatca acttttggtg tatctctttc
    11821 ccaagtatct acaagctgct attcgaaaag gtctttataa ggaatatcat cgattttctc
    11881 ataacgacag tcatgttaag ggagtgattg atgtaagaaa ccatctcaag aaaaatcttc
    11941 ctttcacggg aaatattgcc tacgcaacga gagagttcac ctatgataat cccctcatgc
    12001 agttggtccg tcacactatt gaatacatta agaatcagaa aagcattggt caaggggtac
    12061 tagataatct ctcaactagt cgtgaaaacg tatctgaaat cgtgcgtgta acgccctctt
    12121 ataaactagc tgatcgtgct aagattattc ggggaaatca atctaaacct atacgtcatg
    12181 catactttca cgagtacaga aacttacaag aactttgtct gatgatccta aaccaagaaa
    12241 agcacggttt agggtatcaa gatcaaaaaa tctatggtat tctctttgat gttgcctggc
    12301 tttgggaaga gtatgtttac accttgttgc caaaaggttt tgtacatccc agaaataagg
    12361 ataagacgga tggaatttca gtattttctg ttgggaaacg aaaagtatat ccagattttt
    12421 atgacagaga acgaaagatt gttctagatg caaaatataa aaaactggaa ttgactgaaa
    12481 aaggaatcaa ccgtgaggac ttattccagc tgatttccta ttcttatatt ttaaaagctg
    12541 agaaggctgg actgattttt cctagtatgg agcagtcagt aaatagtgaa ataggaaaag
    12601 tagctggcta tggagctcaa ttgaagaagt ggtctattcg aatccctcag aatgcctcat
    12661 tctatagtac attttgtaaa atgatggaaa attcagaaga aaattttaaa gcgattattg
    12721 atgaagaagt ggggagaaaa taatttcccc tctttctttt atttttaaca caagtaaaac
    12781 ccgaacgata tattttaatt cataaaataa tttgacaaaa actgtacttt gatttataat
    12841 aaattaagga aacgtcggaa aaggcgtagt gatgcaagag cataggtagg tcattacaaa
    12901 agaaacgaga catcgatatg ttaaatgaat ttccaatttt tgattacgaa gatattcaat
    12961 tgattccaaa taaatgtgtc attaaaagcc gtgcagaagc ggatacaagt gtcactttag
    13021 gaaatcacac ctttaaacta cctgttgtgc cagcgaatat gcagacgatt ttggatgaaa
    13081 atgtagcaga gcaactggct aaaggtggtt acttctacat tatgcaccgt tttgatgagg
    13141 caggacgcat tccttttatt aaacgaatgc acaatcaagg gctcattgct tctatctctg
    13201 tcggtgttaa ggattatgag tatgattttg ttagccagct caaggctgat actcctgagt
    13261 atatcactat tgatattgct catggtcatg cggatagcgt gatttctatg attcaacaca
    13321 tcaagaaaga attgccagat acctttgtca ttgctggaaa tgtgggaaca ccagaagctg
    13381 tgcgtgaatt ggaaaatgct ggtgcggatg ctactaaggt cggaatcggt cctggtaagg
    13441 tttgtatcac caaggttaag actggttttg gtacaggtgg ttggcagttg gctgctctac
    13501 gctggtgtgc caaggctgcg cgtaaaccga ttatcgctga tggaggaatt cgtactcacg
    13561 gtgatattgc taagtctatc cgcttcggtg ctagcatgat catgattggt tccctctttg
    13621 caggacacat tgaaagtcca gggaaaacga ttgaagtcga tggtgaacag ttcaaagaat
    13681 attatggttc agcctcacaa tatcaaaaag gtgcttacaa aaatgtggaa ggcaaacgta
    13741 tcttgcttcc tgctaaaggt catctgcaag acactttaac tgagatggaa caagaccttc
    13801 aaagtgctat ttcttatgca ggtggacgtc aggttgctga ccttaaacac gttgattatg
    13861 tgatcgtgaa aaactccatc tggaatgggg atgcttccca ctaagacggg gtctttgacc
    13921 aaaaaacttg ttattagagc aaatttctgt tataataaaa caagtttcca cccttagtgt
    13981 aatggatatc acgtaagatt ccggttcttg agatgggggt tcgattccct cagggtggat
    14041 gtaaacatcc taaaaagcct ttaatagggc ttttttcttt atcctgaccc aaatttgccg
    14101 ctaaaactga gaatctctgt ctaaagaatt cccaaatgct ttccagtccc gttttagagt
    14161 gactcagggg gctttttttg atataataaa aaggactatt atcagttaga aagaggttgg
    14221 tatgaaagaa ttacaaactg tactaaagaa tcattttgca atcgaatttg cagacaaaaa
    14281 gttactggaa actgccttta ctcatacgag ttatgccaat gagcaccgcc tcttaaaaat
    14341 ttcacacaat gaacgcttgg aatttttagg agacgctgtt ctacagttat tgatttcaga
    14401 atatctatat aaaaaatatc ctaaaaagcc tgaaggtgac ctatcaaaac tccgtgctat
    14461 gattgtccgt gaggagagtt tagctggttt tgcgcgtgat tgccagtttg accagtttat
    14521 caagttgggt aaaggggaag aaaaatcagg tggtcgcaat cgtgacacca ttcttggtga
    14581 tgcctttgaa gcctttcttg gtgcccttct tttggataag gatgtggcca aggtcaagga
    14641 atttatctat caagtcatga ttcctaaggt tgaagcaggc gagtttgaga tgattacaga
    14701 ctataaaacc catctccaag agttgcttca ggtcaatggt gatgtggcta ttcgttatca
    14761 ggtgatttct gaaacagggc ctgctcacga taaggttttt gatgtagaag ttcttgttga
    14821 aggtaagagc atcggtcaag gccaaggtcg ttctaagaaa ttagcagagc aggaagctgc
    14881 caaaaatgcc gttgagaagg ggctggattc atgtatttaa aggaaatcga aattcagggg
    14941 ttcaagtctt ttgctgataa gaccaaggtc gtttttgacc aaggtgtgac ggcagttgtt
    15001 ggacccaatg gatctggaaa gtccaatatt acagaaagtc tgcgttgggc tttgggggag
    15061 tctagtgtca agagtctccg tgggggcaag atgccggata tcatctttgc tggaaccgaa
    15121 agtcgcaaac cgctcaatta tgcttctgta gttgtgactc tggataatca tgacggattt
    15181 atcaaggatg caggtcaaga aatcagggtg gaacgccatg tctatcgtag tggagatagc
    15241 gaatacaaga ttgacggcaa gaaagtccgt ctgcgtgata ttcatgacct cttcttggat
    15301 actggattgg gacgagattc cttctctatt atttcccaag ggaaggttga ggagattttt
    15361 aattccaagc ctgaggaacg acgagctatt tttgaagaag ctgctggagt tttaaaatac
    15421 aagactcgca gaaaagaaac cgagagtaaa ctgcagcaaa ctcaggataa tctggaccgc
    15481 ttagaggaca ttatctacga gttggataat caaatcaagc ctcttgagaa gcaagctgag
    15541 aatgcccgta agtttttaga cttggaagga caacgtaagg ctatttattt agatgttctg
    15601 gttgctcaaa tcaaggaaaa taaggctgaa ctagagtcga cagaagaaga gttgactcag
    15661 gttcaagaac tcttgatgag ttattaccaa aagcgtgaaa aattagaaga agaaaatcaa
    15721 actcttaaaa agcaacgcca agatttacag gctgaaatgg ccaaagacca aggcagtttg
    15781 atggacttga ctagtctgat tagtgattta gaaagaaaat tagccctatc gaaactggag
    15841 tccgagcaag tggccttgaa tcaacaggag gcacaagccc gtttggctgc tttggaggat
    15901 aagagaaatt cactcagcaa agaaaagtct gataaagaaa gctctttagc tctgttagag
    15961 ggaaatctag tccaaaataa tcaaaaactc aatcgtttag aagctgaatt gctggctttc
    16021 tcagacgatc ctgatcagat gattgagctc ttacgtgaac gctttgtagc tcttttacaa
    16081 gaagaagcgg atgtctcaaa ccagttgacc cgtattgaga atgagttgga aaatagtcgt
    16141 cagctttctc aaaaacaagc agatcaacta gaaaagctga aagagcaatt agctatagct
    16201 aaagagaagg ctagtcagca aaaggaagag cttgaaactg ccaaggagca ggttcagaaa
    16261 ttattggctg actaccaagc tattgccaag gagcaagagg agcagaaaac ttcctatcaa
    16321 gctcaacaaa gccaactctt tgaccgtctg gataatctca aaaacaagca ggccagagct
    16381 caaagtttgg aaaatatcct gagaaatcat agtaactttt atgcaggtgt taagagtgtt
    16441 ctccaagaaa aagatcgcct aggtgggatt attggtgcag tcagtgagca tctgaccttt
    16501 gatgtttatt atcaaactgc cctagagatt gccttagggg caagtagcca gcatatcatc
    16561 gtagaagatg aagagtcggc aaccaaggct attgacttcc ttaaacggaa cagagccggt
    16621 cgtgcaacct ttcttccttt gaccactatc aaggcgcgta cgatttctag tcagaaccaa
    16681 gatgctatcg ctgtaagccc aggtttcctt gggatggcag atgagttggt gacttttgat
    16741 actagactgg aagccatttt caagaacttg ctagctacga cggctatctt tgataccgta
    16801 gaacatgcgc gtgcagcagc tcgccaagtt cgttatcagg ttcgtatggt gacattggat
    16861 gggacagagt tgcgcacagg tggttcttat gcaggtggtg ccaatcgcca gaataacagc
    16921 atttttatca agccagaact ggagcaatta caaaaagaaa ttgctgaaga agaagcaagc
    16981 ttgggttcag aagaggcgac tttgaagacc ttgcaagatg agatggccag attgaccgag
    17041 tcattagaag ctattaaatc tcaaggagag caggcacgta ttcaggagca aggcttgttc
    17101 ctcgcttatc agcaaactag tcagcaagtt gaagaactgg aaactctttg gaaactccaa
    17161 gaagaggaaa tagatcgtct ttccgaggga gattggcaag cggataagga aaactgccaa
    17221 gagcgtcttg ctgcaatctc cagtgacaag caaaatctgg aagctgagat tgaagagatt
    17281 aagtctaaca aaaatgccat ccaagaacgc tatcaaaact tgcaggaaga gctagcgcaa
    17341 gctcgtttgc ttaagacaga actgcaaggg caaaaacgtt atgaagttgc tgatattgaa
    17401 cgcttaggca aggaattgga caatcttgat tttgaacaag aggaaatcca gcgccttctt
    17461 caagaaaaga ttgataatct tgagaaggtt gatacagaat tgctcagtca acaggcggaa
    17521 gaatccaaaa ctcaaaaaac aaatctccaa caaggtttga ttcgcaagca gtttgagttg
    17581 gatgatattg agggtcaact ggatgatatt gctagtcatt tggatcaggc tcgccagcag
    17641 aatgaagagt ggattcgcaa gcaaactcgt gctgaagcca agaaagaaaa ggttagcgag
    17701 cgcttgcgcc atctacaaag tcaattaaca gaccagtacc agatcagcta cacagaagct
    17761 ttagaaaagg cgcatgagtt ggaaaatctc aatctggcag agcaagaagt taaggattta
    17821 gagaaggcta tacgctcact gggtcctgtc aatttagacg ctattgaaca gtacgaagaa
    17881 gttcacaacc gtctggattt cctaaatagc caacgtgatg atattttgtc tgcgaaaaac
    17941 ctgctccttg agaccattac agagatgaat gatgaggtca aggaacgctt taaatcaacc
    18001 tttgaggtca ttcgtgagtc ctttaaagtg accttcaagc agatgtttgg tggaggtcag
    18061 gctgacctca ttttgactga aggagacttg ctgacagctg gggttgaaat ttctgtccaa
    18121 ccaccaggca agaaaatcca gtctcttaat ctcatgagtg gtggtgaaaa agccctatcg
    18181 gctcttgcct tgctcttctc cattattcgt gtcaagacta ttccatttgt tatcttggat
    18241 gaggtggaag ctgcgctgga tgaagccaat gttaaacgtt ttggggatta cctcaaccgc
    18301 tttgacaagg acagccagtt catcgtcgta acccaccgta agggaaccat ggcagcggct
    18361 gattccatct atggagtgac catgcaagaa tcgggtgttt caaagattgt ttcagttaag
    18421 ttaaaagatt tagaaagtat tgaaggatga caattaaact agtagcaacg gatatggacg
    18481 gaaccttcct agatgggaat ggacgctttg atatggatcg tctcaagtct ctcttggttt
    18541 cctacaagga aaaagggatt tactttgcgg tagcttcggg tcggggattt ctgtctctag
    18601 aaaaattatt tgctggtgtt cgtgatgaca ttattttcat cgcggaaaat ggcagtttgg
    18661 tagagtatca aggtcaggac ttgtatgaag cgactatgtc tcgtgacttt tatctggcaa
    18721 cttttgaaaa gctgaaaact tcaccttatg tagatatcaa taaactgctc ttgacgggta
    18781 agaagggttc atatgttcta gatacggttg atgagaccta tttgaaagtg agtcagcact
    18841 ataatgaaaa tatccaaaaa gtagcgagtt tggaagatat cacagatgac attttcaaat
    18901 ttacaaccaa cttcacagaa gaaacgctgg aagatgggga ggcttgggta aacgaaaacg
    18961 ttcctggtgt taaggccatg acaactggct ttgaatccat tgatattgtt ctggactatg
    19021 tcgataaggg agtggccatt gttgaattag ttaaaaaact tggtatcaca atggatcagg
    19081 tcatggcttt tggagacaat cttaatgact tacatatgat gcaggttgtg ggacatcctg
    19141 tagctcctga aaatgcacga cctgaaattt tagaattagc aaagactgtg attggtcacc
    19201 ataaggaacg gtcggttata gcttatatgg agggcttata atggcagata taaaattgat
    19261 tgcattggac ttggacggga ccttgctgac tactgataaa aggctgacgg atcgtaccaa
    19321 ggaaaccttg caagctgcgc gtgatcgtgg tatcaaggtc gtattgacaa ctggtcgtcc
    19381 cttaaaagcc atggatttct ttctccatga gttagggact gacggtcagg aagatgagta
    19441 taccattact tttaatggtg gattagttca gaaaaataca ggagaaatcc ttgataaaac
    19501 agtcttttca tatgatgatg tggcacgttt gtatgaagaa acagagaaat tatcactgcc
    19561 tcttgatgcc atctcagaag gaacagttta tcaaatccaa tcggaccaag aaagtcttta
    19621 tgccaaattc aatccagctt tgacctttgt tccagtggac tttgaagact tatctagtca
    19681 aatgacctac aacaaatgcg tgactgcctt tgctcaagaa cccttggatg cagccattca
    19741 gaagatttct ccagaattgt ttgaccaata tgaaatcttt aaatcacgtg aaatgttgct
    19801 agaatggtca ccaaagaatg ttcataaagc aacaggtttg gcaaaactaa tcagccatct
    19861 tggaatcgac caaagtcaag tgatggcttg tggtgacgag gccaatgacc tctctatgat
    19921 tgaatgggca ggtcttggtg ttgctatgca aaacgctgtt cctgaagtaa aggcagccgc
    19981 aaatgtagtg acgccgatga ccaacgatga ggaagctgtc gcctgggcta tcgaagaata
    20041 tgtgctaaag gagaactaag atatgggatt gtttgaccgt ctattcggaa aaaaagaaga
    20101 acctaaaatc gaagaagttg taaaagaagc tctggaaaat cttgatttgt ctgaagatgt
    20161 tgatcctacc ttcacagaag ttgaggaagt ttctcaggaa gaagcagagg ttgaaattgt
    20221 tgaacaagct gtgttccaag aagaggaaat ccaagacaca gttgaagaaa gtcaggattc
    20281 agagccagct gtaggggttt ctcaagaaga aatagaagaa ttaccaaact cagaagaatt
    20341 accagaggaa gagaagcttg agcacgaagg aactgtagaa gaaaataatt ctgaagtgct
    20401 tgaaccagaa gctcctcaaa cagaagaaac tgttcaggaa aaatatgacc gcagtcttaa
    20461 gaaaactcgc acaggtttcg gtgcccgctt gaatgccttc tttgctaact tccgctctgt
    20521 tgacgaagaa tttttcgagg aactggaaga actgctgatt atgagtgatg ttggtgtcca
    20581 agtcgcttct aacttaacgg aggaactacg ttacgaagcc aagcttgaaa atgccaagaa
    20641 acctgatgta cttcgtcgtg tcatcattga gaaattggtt gagctttatg aaaaggatgg
    20701 tagctacgat gaaagcatcc acttccaaga taacttgaca gttatgctct ttgttggtgt
    20761 gaatggtgtt gggaaaacaa cttctatcgg aaaactagcc caccgctaca aacaagctgg
    20821 taagaaggtc atgctggttg cagcagatac cttccgtgcg ggtgcagtag ctcagctagc
    20881 tgaatggggc cgacgagtag atgttccagt agtaactgga cctgaaaaag ctgatccagc
    20941 cagcgtggtc tttgatggta tggaacgtgc cgtgactgaa ggtatcgata ttctcatgat
    21001 tgatactgct ggtcgtctgc aaaataagga taaccttatg gctgagttgg aaaagattgg
    21061 tcgtattatc aaacgtgttg tgccagaagc accacatgaa accttcttgg cacttgatgc
    21121 atcaacaggt caaaatgccc tagtacaggc caaagaattt tcgaaaatca cacctttaac
    21181 gggaattgtt ttgactaaga ttgatggaac tgctcgagga ggtgtggttc tagccattcg
    21241 tgaagaactc aatattcctg taaaattgat tggttttggt gaaaaaatcg atgatattgg
    21301 agagtttaac tcagaaaact ttatgaaagg tctcttggaa ggtttaatct aatcagaagc
    21361 aaaaatcctg caaggcataa acttgcagga aattttttta ttctaagcga ccatcttgac
    21421 gataggtgat atctggttgc caagtccatt tggcaccgaa tttttcaagt aggtcaaagc
    21481 tggcttgagg tcccatgctt ccagctttat agtcatgaag tggggcacca ttttcagccc
    21541 agagcttttc aatacggtca atcaacttcc atgacgcaca aacttcatcc cagtggctaa
    21601 agttagttga gttgttattt aggacatcat aaatcaattt ttcgtatggt tctggagaag
    21661 caccagttgc agtcgcatct gtacggtaat caagtgagtt aggagccaag ttaaattctt
    21721 ctcctacttg cttcccattt aggctaagag agaagccttc tgttggttga atatagatgg
    21781 tcaaaatatt tggagcaagt ggttctccaa agatagaatc catttgttta aagacgatgt
    21841 tgacatgagt tcctttttca gtcagtcgtt tacctgtacg gaaaaagaaa ggaacaccac
    21901 ggaatcgatc gctgtctaca aagaaggcac cagatgtaaa ggtttcagtt gttgattctg
    21961 gattcacatt tggctcgcta cgataagaga tgtatttcat gccatcaatc ttaccagagc
    22021 ggtattgccc acggataaag tgttctttga gttcttcatc agttggatga tagaggtttt
    22081 taaagacctt aatcttttca gcacgaatct cgtcttttgt gaagcttgct ggtttgtcca
    22141 tggcgaggag cgaaagaagt tgtagagtgt ggttttggac catgtcacgg agggcaccgg
    22201 attggtcata gtagccacca cgttcttcta cacccaagcg ctccgcaaag gtaatttgaa
    22261 cattgtcgat aaaatccttg ttccaaacgt tttcaaaaat caagtttgca aagcgaactg
    22321 caaagatgct ttggatcatt tccttaccaa gataatggtc gatacggaaa atttgttctt
    22381 cgtcaaatgt tgctaggagt tcgtcattca acttgcttgc agttgcgtaa tctgtaccaa
    22441 atggtttttc aacgatcaag cgctcaaaac ctttgccatc gacaatgttt tcagatttga
    22501 ggtgtttggc aatggttcca aagaactgag gtgccataga caagaagaag agcttattgt
    22561 gttcagcttg gtatttttca ttaagctcag cttgtaattg acgcaaagca atataatgtt
    22621 ccgaatcatt gacatcatgg ctttgatagt agaagtggct agcaaattct tgggcttgct
    22681 cggtactatc tgccaaatca aggatggact cgacaactac agattcaaaa tattccttac
    22741 tccaaggtct acgggcagtt ccaataacgg caaagtgctt ggaaagattg ccggattgat
    22801 atagtctaaa aagggaaggg tagagcttgc gtttagccag gtctccactc gcaccgaaaa
    22861 ttgtaacaat aaccttagat gacatctagc tacctaattt ctatttttat tcctagtctt
    22921 gctaggtatg aggaaacctc atttcataaa cttaattcta acataatttt ccgaaaaatc
    22981 aaaaattcca atacgaggta gaaaaactgc aaggaaatcc ttacagtttg agtttagacg
    23041 tttaagacct tatctaagaa ctctttcaga cgagggtgtt gtgggttatc aaagatttgg
    23101 tcaggtgttc cgtcttcaag gaactcgcca tctgcagtaa agataacgcg gttggcaacc
    23161 tgacgagcaa atcccatctc atgggttacg ataatcatgg tcatgccttg ctcagccaat
    23221 tccttcataa cgttaagtac gtctccaacc atctcagggt caagggcaga agttggttca
    23281 tcgaagagca tgatgtctgg attcattgct aggccacgag cgatggccac acgttgtttt
    23341 tgaccacctg ataggctatc tggattggca ttagctttat ctgctagtcc aaccttttca
    23401 agcaactcca ttcccaattc ctcagcttct tccttagtca tcaacttgtg ctcaatagga
    23461 gcaaaggtga tgttgtccaa tacagacata tgagggaaga ggttgaagtg ttggaatacc
    23521 atgccgatat tttcacggac gtggtcaaca ttggttgttt tttcagttaa atcatagccg
    23581 ttcacagtga tgtgaccgct agtgacttct tctaaaagat tgaggctacg gaggaaagtt
    23641 gacttaccag aacctgaagg accgatgata caaacaacat ctccttcata gaacttagtc
    23701 gtaattcctt ttaggacttc attttttcca tagtgcttgt gtaaatcatt tacatcaatt
    23761 tttaattttg ccattaacga atcctctttt ctaagcgttt cgctagtcta gtcaaaagtg
    23821 tgataattac aagatagaag atagcaagga ttgcatacat cttgaaactt tggtagttac
    23881 gagcaatgat aatcttacca gtttggaaga gttcaaccaa accgatagca gatacgatag
    23941 ttgtatcttt aagagcgata acgaattggt tgacaaagtt tggcaacatc aatttagttg
    24001 cttgtggcaa gataatctta cgcatggttt ttccataaga gatacccaag cttcggctgg
    24061 cttccatttg gccaactgga acggcctgaa taccaccacg aacgatttca gcgatataag
    24121 cagccgcatt gagtgagagg gcaatggttc cagctacaaa gtcgttaatt gggctttgtt
    24181 ggcctgtgat agactcgatg aagtttggaa ttccccagaa gatgaaggct gcaagaatca
    24241 tcaatggaat accacgaata acgtcaacga aaatctcaga gatgacgcga agagatttgt
    24301 atgggctaac gctaaacata ccgaagataa ttccgatgac aatggcaata gcaaatgaga
    24361 taagagctag agcaagagtg ataccaagac cgctaaggag ttgtttgtag ttgttttgaa
    24421 gcaagcccca gagcgttgtt tcgtcaacag tacttgttga agcagttgaa gattcgctag
    24481 ctaggtattt gtcaagaatc ttttggaatt caccgtttgc tttaaggttt gcaagtccgt
    24541 tgttgaacat ttcaatcagt tctggatttg ctcctttttt aacggcaaag gctgtttcac
    24601 cgattggagt tccagagatt ggagttttca atttttgacc ttggctgata gaatatttga
    24661 gaacaggttc atcatccata acggcatcaa tggcaccagt gtttaaactg tcatacattg
    24721 aagaaccatc agcaaaggtt ttgattttgt agccgtattt gctttgattt tctgttagga
    24781 aggtttgaga agcagttccg tttttaacac cgactgtctt tccttttaga tcttcataag
    24841 aagcaatatt gcttgattct ttgacaccaa gaatggtatt agcagtgtag tatgattctg
    24901 agaagtcaaa agttgcctta cgagcatctg tgacagacat accagcgatg ataccatcgg
    24961 cttgaccagc ttggacagca ctgatagcag catcaaaacc agggttggtg atttcaattt
    25021 caaaaccttg gtctttagcg attgccttaa tcaattccat atcaatacca gtgtattggt
    25081 tgcttgaatt ttggaaaaca aaaggggcaa aagaagaatc gctggcaatg atatatttag
    25141 ccttaacagg aatagctttt aatcctgcga gagtagttgt agttggcact gctgaagatg
    25201 atgaagcagt ccatttcttg ataattttat caagactacc atcttttttc atttcagaca
    25261 aggcttggtt aaattcagta accaggtgct cgtatttact tccttttttc acaccgaaag
    25321 caaaacttcc tacagcttca ccatccattt caatatggag gtcttgacct tggttaatgg
    25381 catattcgat aacaggtttg tcatccatca tggcatcgat ggcaccagca ctcaagctgt
    25441 tgttcattaa atcaccagtg tcaaatgttt taatagtaaa gccgtattta tctttgattg
    25501 tttcaaggaa acgttgagcg gcagttccgt ttttaacacc aacggttttg ccagttaatt
    25561 ggtcgtactt gctaattttg tgtgactttg tagtagcaat gacaactttt gtatcatagt
    25621 aagtatcaga catggtgaag actttttcac gttctttagt ctttgtcatc cctgccatga
    25681 tagcgtcggc ttgcccagct tgaaccgcat tgactgctgc gtcaaatcca ggataggaca
    25741 tctgaatgtt ccagccttta atctcagcga ctttgttaat aatgtcaaca tcaattcctt
    25801 tataagtttg atctgaatct ttaaactcaa aaggtgcata ggcggtatca gaaacaatct
    25861 taatcgtttc tgctttggca atacctaatg agaaaattgg gaataaaatt agcaaaaatg
    25921 ctagaaattt tttcttcatt tttagtctcc ttttccgaat attctctcat tatatcagaa
    25981 aaagtaaaaa aaggcaaatt tttatatttt ttgtgtcaga aaatataaca tcgatttttt
    26041 cctttccttg aattgctatt aaaaagtgct ataataatag tatatataga agaaaagagg
    26101 acggggatat gaagaacaaa agaatattta aagacttcca agcttcaaaa atgagtttaa
    26161 acatttacac aagccccttg ttagcctttg tttttgtctt cataggagag tttgtggctt
    26221 ttactttgta tggtattggc ttgttagctc tcatcggact tgctagaaat tttggagagg
    26281 ctggtcaaaa tcttgcaagc tacttgcaga ccttgcatca gagcttgacg gataaaacaa
    26341 gtgactttcg tttaatttta ggattactgg cctttggttt tattcttaac actgtgttca
    26401 gatggacaag aaaagttgag aaaagaccta ttcgaacctt gggattttat agagagaatt
    26461 tcctcagcaa tcttctgaaa ggatttagtc taggcctggc actttttctt ctgaccttgt
    26521 taggtttagt ggtcttaggt caatatcgtt tggaatccat tcacttgaat ccttattctc
    26581 ttgcctttgt cgtctttact atcccatttt ggattttaca ggggacagca gaagaagtgg
    26641 tggcccgtgc ttggctactt cctcaattgg cctcaagaac caatctaaaa ctagctattc
    26701 ttatatctag cctgttcttt accctgcttc atatgggcaa ttctggtctc acccctctat
    26761 ctctagtaaa tctcttttta ttcggagttg ccatggctct ttaccttctc aaaactgata
    26821 cagtttgggg tgttgcaggt attcatggtg cttggaattt tgctcagggt aatctctttg
    26881 ggattttagt tagtggtcaa ccgtcaggaa cgtctctgat gaccttttta ccacaaggca
    26941 atcaagattg gctatcaggt ggttcttttg gcatagaagg ttccattatg acaagtctgg
    27001 tattactact gctgattgtc tatcttgcta ataaattaaa gaaagaaaat gaaaggatgt
    27061 gacttcggtc cgtccttttc ttcgtgaaaa tactataagt atgctaaaat aggaatagca
    27121 catggagaga ggattcttat gatcaatcac attacagata atcaatttaa actagtatca
    27181 aaatatcaac catcaggaga tcaaccccaa gctatcgagc agttggtgga taacattgag
    27241 gggggagaaa aagctcagat tctgatgggg gcgactggaa cagggaagac ctatactatg
    27301 agtcaggtca tttctaaagt caataaacca actctggtta ttgcccacaa taaaactctg
    27361 gctggtcagc tctatgggga gtttaaggaa tttttccctg aaaatgcagt tgagtatttc
    27421 gtatcctact atgattatta ccagccagag gcctatgtcc cttctagcga tacctatatt
    27481 gagaaggata gttctgtcaa tgacgagatt gacaagcttc gccactcagc tacctcagcc
    27541 cttttggagc gtaatgatgt tattgtcgtg gcctcagtct cttgtatcta tggtttgggt
    27601 tcgcccaagg aatacgctga tagtgtcgtt agtctccgtc ctggtctaga gatttctcgt
    27661 gataaactct tgaatgactt ggtcgatatt cagtttgaac gtaatgatat tgatttccaa
    27721 cgcggaagat ttcgcgttcg tggggatgtg gtagagattt tcccagcttc ccgagatgaa
    27781 catgcctttc gagtagaatt ttttggagac gaaattgacc gtattcgtga agttgaggct
    27841 ctgacaggtc aggtgttggg agaagtggat catttagcga ttttcccagc gacacacttt
    27901 gtgaccaatg acgaccacat ggaagttgcc attgcaaaga ttcaggccga gttggaagaa
    27961 caattagctg tctttgaaaa ggaaggtaaa ctgcttgaag cccagcgttt gaaacagcgg
    28021 acagagtatg atatcgaaat gttgcgtgag atgggctata ccaatggggt tgaaaattat
    28081 tctcgccaca tggatggacg gagcgaagga gagcctcctt atacgcttct cgacttcttc
    28141 ccagatgatt tcttgattat gattgacgag agtcatatga ccatagggca aatcaagggc
    28201 atgtacaatg gagaccgttc gcgtaaagaa atgctggtta attatggttt ccgtttgccg
    28261 tctgctttgg acaatcgtcc tctccgtcgg gaggagtttg agagtcacgt tcatcagatt
    28321 gtttacgttt cagcgacacc tggtgactat gaaaatgaac agaccgagac agtgattgag
    28381 caaatcattc gtccaacggg actcttggat ccagaggtgg aagtccgtcc gactatggga
    28441 cagattgatg acctcttggg tgaaatcaat gcccgcgttg aaaaaaatga gcgtaccttt
    28501 atcacaactt tgaccaagaa aatggcagag gatttgaccg actacttcaa ggaaatgggt
    28561 atcaaggtca agtacatgca ctcggatatc aagaccttgg aacggacgga gattatccgt
    28621 gacctgcgct tgggtgtctt tgatgtcttg gtcggaatta acctgctccg tgaaggaatt
    28681 gacgttcctg aagtgagcct cgtagctatt ctcgatgctg acaaggaagg tttccttcgc
    28741 aacgaacgtg gactcatcca gaccattgga cgtgctgcac gtaatagcga aggtcatgtt
    28801 atcatgtatg cggacacggt tacccagtct atgcaacgtg ctatcgatga aactgcccgc
    28861 cgtcgcaaaa tccagatggc ctataatgaa gaacatggta tcgttccaca aaccatcaag
    28921 aaagaaatcc gtgacttgat tgctgtgacc aaggcagttg ctaaggaaga agacaaggaa
    28981 gtcgatatca atagcctcaa caaacaagag cgcaaagaac tagtcaaaaa gcttgagaaa
    29041 caaatgcaag aagcagttga agtgcttgac tttgaactag cagctcagat tcgtgatatg
    29101 atgctggaag tcaaggcctt ggattagggg aatagtatga tttatttaag aaagttaaag
    29161 aaagaagatt tgatgtcttt atgggaaatg gcttattcac agcttaatcc agtttggaaa
    29221 cagtatgatg ctccctatta tgatgattat cagtattttt caaattttaa agaattcgaa
    29281 ctacaaaaat cagaatccat tttaagcaac tcaaatcgcc ttggtatttt tgttgatgat
    29341 aaactagttg ggactgtttc gcgttattgg gtatgtaaag aaacaagatg gatggaattg
    29401 ggaattggta tttatgataa aaaattctgg aacactggta ttgggaaagt tgctatgttg
    29461 cagtggatag ataggacgtt tcaggattac ttggagttgg agcatctggg tttgacaact
    29521 tggtcaggaa atattggtat gatgaaactt gctgaaaaat taagaatgaa aaaagaagct
    29581 catattccaa aagttcgtta ttatcaaggt aaatattttg acagtattaa atatggtatt
    29641 ttgagagaag actgggagaa aataaatgac ggttattatc aaatcaatgg aaactcctga
    29701 agagatagaa ggtaaatcct tcgttcactg gcaaacgtgg agagaggctt atgatgacct
    29761 tttgcctgcg gaatttcagg agacaatgac attagaaaga tgtcgactct ttagtcaaaa
    29821 gtatccagaa aatacattga ttgcgatgga tggtgtgaag atagttggtt ttataagtta
    29881 tggcaactgt cgtgatgaga ctattcaagc tggtgaaatt attgctttat atgttttaaa
    29941 agactattat ggaaaaggaa tcgcacaaaa gttagtgaaa gcagctttga ctgatcttaa
    30001 tcatttttct gaaattttct tatgggtatt gaaagataac aagcgcgcca ttgctttcta
    30061 tcaaaaaatg ggttttactt ttgatggaca agaaaaaata cttgaacttg gaaagcctat
    30121 aaaggaaaaa cggatggtat tctattctaa ataattctca aaagtaaaag ctaatatggt
    30181 accaagtctg aaaatttaat aaattagaaa gcgagtaaat ttatgtcccg ttcccaatta
    30241 acaattttaa caaatatctg tctgattgaa gacctcgaaa ctcagcgcgt ggtgatgcag
    30301 tatcgcgccc ctgaaaacaa tcgctggtct ggttatgcct ttcctggagg tcatgtagaa
    30361 aatgatgagg cttttgcgga gtctgtcatt cgtgaaatct acgaagaaac agggttgact
    30421 atccaaaatc ctcaacttgt cggcattaaa aattggccac tagatacagg tgggcgctat
    30481 attgtcattt gttataaggc gactgagttc tctggtaccc ttcaatcttc agaagaggga
    30541 gaagtttctt gggtgcaaaa agaccagatt ccaaacttaa atctggccta tgatatgcta
    30601 ccattgatgg aaatgatgga agctcccgac aagtcagagt ttttctaccc tcgccgtaca
    30661 gaagacgatt gggaaaagaa aatcttctag tcttttacta aataacctag ctgatccaag
    30721 gcctcctcga tatagtggag gtcttgttgt gtctcggctt caactaggtg ataatgaata
    30781 ccatctgtta actcagaaat tggcttaaag tcagaacgtt caacttgttc tagaaaatgt
    30841 tgcacgtcgc ggcgacaggt cagttttagt aaggtttcaa tctctccata aacaggatga
    30901 tcaatcaaga tattttgaac gcgaccacca ttatctacga tagcaagtaa ttctcgtcca
    30961 atttcttcaa cttcatgctt gaccttaaat aatttgtgat gataagtatt tgcattagca
    31021 tctttataga tataaccacg attggtagat agaattggag atccatcagc tcttaaaatt
    31081 gcaatatctt gaacaataac ttgtcgagtg acatgaaagt gctcagctaa tctttggcca
    31141 ttgagggctt taggagcttc tttcaagagt tggagcaggg cttgtttgcg atcttttgtc
    31201 atagttcttc cttttaacgg cgttttcgaa gcactttata gacagctagt gctaatgtat
    31261 agtctaccat actatggata attgtaccaa atccaactag tacaaataga acataaaaca
    31321 tattttctac attggtacca gaagttgcgt aaaaaacgac acaggccaat acttcagcaa
    31381 gggcatgaac aacagccaaa acaaagttga aaatccagga agattttggt ttatctaggg
    31441 tatcggggaa tttttgtagg taaagagctc ctaaagcacc aaaagatata tgggaaaaag
    31501 cccgaaaaac gataaccatg ggatagccag ccatcaaaaa tccaaaacta gaggctagga
    31561 tgacaaaaac tgccatcaag ggcgacaaga acatggctat aaaaatagcg atgtggctcc
    31621 ccaaagtata ggaagcaggt ggaatgacaa tcttgaaagg cataacaatt ggaatcaaaa
    31681 tcgcaatagc cgttaaaagg gctgtcattg tcataaattg tgtctttttc cgtgtattca
    31741 caagaatctc ctttttaact gcatatacac tagtatggta caataaacca gacaataaag
    31801 caagaattta cttgggttta tagatcattt tttagttaaa agttatagta gattgaaact
    31861 agaatagtcc acctctactt ctaaaacatt gttagaaatc gatttggctg tcctgatcga
    31921 tttgtcctgt tcttatttcg ttttactata gtaaagattt cattaaaaag aaactgtata
    31981 gagcaaaatc tccaccttca ggtttgaaag cggagattgt ttttattttt tcaaggtttg
    32041 tagtcgtgga acaatggcta gggttagaat tgcagaaatg accaattcag caattgaatt
    32101 tgttgagata acggttgcca gaagaagttg gatatttcca ttataaacat ttccaaatag
    32161 gaagaagatt cctccaagga caaagatagt atttgtcaag gaaccaaggg ctccagctaa
    32221 aatcagacca gtcttgtttt tcatcagttt atagactaag taaggagtta aaccaatcaa
    32281 aatacgtggg acgatggcaa tgatagctga gtagatgttt ccgtttggta cgaagggaga
    32341 gaagaggtag cttgtcggta gaatcgtaat cgtgttaacc gtcaagctaa gtaatcccat
    32401 caaaaatcca agtgtaaccc caactcgtgg accataaata atgctggcaa taatgacagg
    32461 aatatgaaca atggtcggtt tgattggaaa tggaaaaagg ttaaagataa gtgagctcag
    32521 aaagtgtatc acgagcatgg tagcaaaaaa gatagcaatg ggtgcaatat tagagcgttt
    32581 tttcatcgat agtttccttt attctttcta aaataattgt gaggtcagct aaagctcctc
    32641 gtccgtggtc tccacaagct agtagggatt ccttaggagc aatcagctga tagccgtagg
    32701 tttctaatgt tttcagatta ttctgagtta ctggatggtc atacattttt gtattcatag
    32761 caggagctat tagttttgga atatgacttg gtagggctag agctgtactg gttaccatgt
    32821 tgtccgcaaa tccgtgagct agttttgcaa tagtgttagc agttgcaggt accacgataa
    32881 ataaatctgc tttttttcca agttcgatat gattgacctg atcaggatag ggttccttca
    32941 tgacatccaa gtggacagga ttctgtgaga gtacctgtag tgtcaaaggt tggataaact
    33001 ctgtagcagc ctgagtcatt aagacagtga cttgatggcc ttgttttttt agagaactga
    33061 ctaaatctgc cgacttataa gaggcgattg aacccgttac agccaagaga atgtttgcca
    33121 tagttttcct ttctaagaat gataggcttg aattttttca aggaggagtt ctgcaatttc
    33181 ttctttagtc tggactgttt gaagctgatt tttctcaaca aatatagctc ggtgctgatc
    33241 tgctgaaatt tgagtcaggt cattcgcgat gattaaatct gcttgattct tgataagact
    33301 ttttcgtgca atgtcaacca gatgatcttc ggtaacatca accagcagtt tgaaaccaat
    33361 cagatgaata gtaggattcc attccttgac tagggatatg attttgggtg tctttttaag
    33421 gaacaaaacc tgaacctcat cagttgaaga aatcttggcc tgatgatttt gcttgcttaa
    33481 aaattctttt agattggagc tagcctgaac ttcctcaagc cctgtcatat aaacaggagt
    33541 gtagtcagaa acagccattg agtggatcaa gacctgataa tcctgaacac gttcttgcat
    33601 ttctattaga aggtccttgg tattggtaat ttctcgaata cttaggttag gatgaggctc
    33661 tggcttcaga gctcgttttg tcgtaattaa acaaacttca taccctgcag aaagcaaagt
    33721 ctctgtgata attttcccca agtgacctgt agaatggtta gtgatagagc ggacgctatc
    33781 gatagcttca ctggtaccgc ccgatgtaac taaaattttc atagcactat tgtacacaaa
    33841 aataagcggt aagtaaaatg aaagtgataa atttttggta aaaacgaaga tttattatag
    33901 tttcaaacta taaagccata taaaatttaa gaaaggactc taaaagtctt aaaaacagag
    33961 atatttacga acgtttagag agttttagga tgttaaaaat cttgttttgt gatataatga
    34021 attatcatat aagaggttag aggaattttg aatgaaaaca gatattgaaa tcgcacagag
    34081 tattgagttg aagccaattg ttgatgttgt agagaaactt ggtatttctt atgacgattt
    34141 ggagttgtac ggaaagtaca aggctaaact cagctttgat aaaattcgtg cagttgagag
    34201 caatccagtc ggtaaattga tcttggttac tgccatcaac ccaacacctg caggtgaagg
    34261 aaagtcgacg cttaccattg gtcttgcgga tgccttgaac aagattggca agaaaaccat
    34321 gattgctatc cgcgaaccgt ctcttggtcc agtcatgggg atcaagggtg gtgctgctgg
    34381 tggtgggtat gcacaagttc tgccaatgga agacatcaac ctccacttta ctggagatat
    34441 gcatgctatt acaactgcca acaatgccct ttctgccttg attgacaacc acttgcacca
    34501 agggaatgag ctgggaattg atcaacgtcg tatcctctgg aaacgcgttg tggacttgaa
    34561 cgaccgtacg ctccgccatg tgactgttgg tcttggtggt cctctaaacg gtattccacg
    34621 tgaggatggt tttgatatta cagttgcttc agaaatcatg gcaattcttt gcttggcaac
    34681 ggacatcgag gacttgaaac gtcgtttggc gaatatcgtt attggttatc gctatgaccg
    34741 tacgcctgtt tctgtaggtg atttgcaggt tgagggtgcc ttggctttga ttttgaagga
    34801 tgctattaag ccaaacttgg ttcagacaat ttacggtaca cctgcctttg tacacggtgg
    34861 tccatttgcc aatatcgctc atggctgtaa ctctgttttg gcgacgacaa cagcccttca
    34921 cttggctgat tacactgtta ctgaagctgg ttttggtgcg gaccttggtg ctgagaaatt
    34981 ccttgatatc aagacaccaa acttgccaac atctccagat gcagttgtta ttgtcgcaac
    35041 ccttcgtgcc cttaagatga atggtggtgt ggctaaagac gctctgactg aagaaaatgt
    35101 agaggcagtt cgtgcaggtt ttgctaactt gaaacgccac gttgaaaata tccgtaagtt
    35161 cggtattcca gcagttgtag ctattaacga atttgtatct gatacagaag ctgaaattgc
    35221 agccttaaaa gaactctgtg cctcaatcga tgtaccagtt gaattggcta gtgtctgggc
    35281 tgatggagca gaaggtggag tagcacttgc cgaaacagtt gttaagacaa ttgctgaaaa
    35341 tccagctaac tataaacgtt tgtatgataa tgacctttct gtccaagaaa agattgaaaa
    35401 aattgtcact gaaatctatc gtggtagcaa agtgaacttt gagaagaaag ctcaaacaca
    35461 aattgctcaa atcgttcaaa acggttggga caaattgcca atctgtatgg ctaaaactca
    35521 atacagtttc tcagacaatc caaatgcgct tggagcacca gaaaactttg aaattaccat
    35581 tcgtgaattg gtaccaaaat taggtgcagg cttcatcgtt gccttaactg gtgatgttat
    35641 gaccatgcca ggtcttccaa aacgtccagc agctctcaac atggatgttg aaagcgatgg
    35701 aactgtacta ggcttgttct agtggattga aattgacttt aagtgagttt ggaaataata
    35761 tccaagctca ttttttctca gactgcaagc agttcatcaa taagatttat actcttcgaa
    35821 aatctcttca aactacgtca gcttcacctt gccgtaggta tatgatactg actctgtcag
    35881 ttctatccac aacctcaaaa cagtgttttg agcaacctgc ggctagcttc ctagtttgct
    35941 ctttgatttt cattgagtat aagtagatag aaaagttata tactatctat tgaaaatatt
    36001 ataaatcaga tacaaagaca ggtgaaacct tctgtgttat actagagata tgttagattt
    36061 gaaagaatac ggtatcgtca tgtggccgga ggagaaagtc atttctttcc gtgagaaact
    36121 tctcgcttgg tatgatgaaa acaaaagaga tttgccttgg aggagaagta aaaatcctta
    36181 tcacatctgg gtatctgaaa tcatgcttca gcagaccagg gtggatacag ttatccctta
    36241 ctacgaaaga tttttggact ggtttccaac tgtcgaaagt ctggcaactg cgcctgagga
    36301 gagtttactg aaagcttggg agggcttggg ctattattct cgagttcgca atatgcaggc
    36361 tgcagcccag cagattatga ctgactttgg tggccaattt ccaaatacct atgaaggaat
    36421 ttccagcttg aaagggattg gaccttacac agcaggagcc atttccagta ttgcttttaa
    36481 cttgcctgag ccagctgtag atggtaatgt catgcgggtc ttggcgcgtc tgtttgaagt
    36541 caaccacgat attgggattc caagtaatcg aaaaattttt caggcaatga tggagatctt
    36601 gattaatccg gatcgtccgg gtgattttaa tcaagccttg atggacttag ggtctgatat
    36661 tgagtctcct gtaaatccca gacctgaaga aagcccagtc aaggacttta gtgcggcata
    36721 tcagaatgga accatggacc gttatccaat taaatctcct aagaaaaagc cggttcctat
    36781 ttatcttaaa gccttagttg tgaaaaattc tcaaggacaa tttttacttg aaaaaaatga
    36841 aagcgaaaag ctcttggcag gtttttggca tttccccttt atagaagttg ataacttttc
    36901 gcaagaagag cagtttgacc tctttcatca ggttgcggaa gaaagtgtga actttggtcc
    36961 cagtccagaa gagagtttcc agcaggacta tgacctagat gttgattggc ttgatgtttg
    37021 ttttgataca gtccagcatg tttttagtca tcgaaaatgg catgtacaga ttgtagcagg
    37081 tcaggtgagt gacttccatg atttttcaga tagggaagtt cgctggcttt caccagaaga
    37141 attcaagaat tacccacttg ccaaacccca acaaaaaatc tggcaggttt atgcacaagc
    37201 caacttagac agtagccaag actagctatt gtgtcttctg actatttttt tggtataata
    37261 gtagagaaaa aggtgaacat atgaaaaaaa tactaattgt agatgatgag aaaccaatct
    37321 cggatattat caagtttaat atgaccaagg aaggttacga agttgtaact gcttttaatg
    37381 gtcgtgaagc gctagagcaa tttgaagcag agcaaccaga tattattatt ctggatttga
    37441 tgcttccaga aattgatggt ttagaagttg ctaagaccat tcgtaagaca agcagtgtgc
    37501 ccattcttat gctttcagcc aaagatagtg aatttgataa ggttatcggt ttggaacttg
    37561 gggcagatga ctatgtaaca aaacccttct ccaatcgtga gttgcaggcg cgtgttaaag
    37621 ctcttctgcg tcgttctcaa cctatgccag tagatggtca ggaagcagat agtaaacctc
    37681 aacctatcca aattggggat ttagaaattg ttccagacgc ctacgtggct aaaaaatatg
    37741 gcgaagaact agacttaacc catcgtgaat ttgagctttt gtatcattta gcatcgcata
    37801 caggtcaagt catcacgcgc gaacacttgc ttgagactgt ctggggttat gactattttg
    37861 gtgatgtccg tacagttgat gtgactgtac gacgtctgcg tgagaagatt gaagatacgc
    37921 ccagccgacc agagtatatc ttgacgcgcc gtggtgtagg gtattacatg agaaataatg
    37981 cttgatttac tgaaacaaac catttttacc agagatttta tctttatcct gattttgtta
    38041 ggtttcatcc ttgttgtgac cctcttatta ctggaaaata gacgtgataa tattcagttg
    38101 aagcaagtca atcaaaaggt taaagatttg attgcaggag attattccaa ggttcttgat
    38161 atgcaaggtg ggtctgaaat caccaatatt accaataatt tgaatgactt gtcggaggtt
    38221 attcgtctca ctcaggaaaa tctagaacaa gagagtaaga ggctaaatag tattctgttt
    38281 tatatgacag atggggttct tgcgactaac cgtcggggtc agattatcat gattaacgat
    38341 acagccaaga agcaactggg gttggttaag gaagatgttc tgaatagaag cattttggaa
    38401 ttgctcaaga tagaagaaaa ctatgaattg cgtgatttga ttacccaaag tccagaattg
    38461 ttgctagatt cccaagatat caatggcgaa tatttgaacc ttcgagttcg ctttgccttg
    38521 atacgtcgag agtctggctt tatttcaggt ttggtggctg ttttgcatga tacgacggag
    38581 caggagaagg aagaacgcga acgaagactc tttgtttcca atgttagcca tgagttacgg
    38641 actcctctga ctagcgtaaa atcctatctt gaagccttgg atgagggggc tttgtgtgaa
    38701 actgtagcac cagactttat caaggtttct cttgatgaga ccaaccgtat gatgcgcatg
    38761 gtgacggatc tcctccatct ttcacgtatt gataatgcta ccagtcacct agatgtggaa
    38821 ctgattaact tcactgcttt tattaccttt atcctcaatc gttttgacaa gatgaaagga
    38881 caggaaaagg agaaaaaata tgagttggtg agagattatc ccatcaattc tatctggatg
    38941 gaaattgata cagataagat gacgcaggtt gtcgacaata ttttaaataa tgctattaag
    39001 tattcgccag atgggggtaa aatcactgtc agaatgaaga caactgaaga ccagatgatt
    39061 ttatccattt ctgaccacgg tttggggatt cctaagcagg atttaccacg tatctttgac
    39121 cgtttctatc gtgtggatcg tgctagaagt cgtgcacaag gtggtacagg tctaggactg
    39181 tctatcgcta aagaaattat caaacaacat aagggcttta tttgggccaa gagtgaatac
    39241 ggcaagggtt caacctttac cattgtactc ccttatgata aggatgcagt gaaagaagaa
    39301 gtatgggagg atgaagtaga agactagaat gagtgaaata ggctttaaat acagtatttt
    39361 agcgtcgggt tccagtggaa attcttttta tctggaaacc tcaaaaaaga agcttttagt
    39421 agatgcaggc ttgtctggca agaaaattac cagtctgcta gctgaaatta accgtaagcc
    39481 agaagacctg gatgccatct tgattaccca tgagcattca gatcatatcc atggagtagg
    39541 cgttttggct cgcaagtatg gtatggatct ttatgccaat gaaaagacct ggcaagctat
    39601 ggaaaatagt aaatatcttg gcaaggtgga ttcttcgcaa aagcatattt ttgaaatggg
    39661 taaaaccaaa acctttggag atatcgacat cgagagtttt ggtgtaagcc atgatgcagt
    39721 cgcaccgcag ttctatcgct ttatgaagga tgataagagt tttgtcctct tgacagatac
    39781 aggttatgtc agtgaccgta tggcgggcat tgtcgaaaat gcggatggtt atcttatcga
    39841 ggccaaccat gatgtagaga ttttgcgatc aggttcttac gcttggcgac tcaaacaacg
    39901 aatcctatct gaccttggtc acctttctaa cgaggacggt gctgaagcta tgattcggac
    39961 gctaggaaat cgtactaaga agatttacct tgggcattta tctaaggaaa acaatatcaa
    40021 ggaactggct catatgacca tggtcaatca gctggctcaa gctgatctgg gagtcggagt
    40081 agactttaag gtttatgata cctcaccaga taccgcaaca ccattgacag agatatagaa
    40141 agaacgctga gaaggtgttc tttttatatt gactgaacac ctaaaaagta atacaatggt
    40201 gttaccatta aaaaagggag cacaaaagat gactactatt acattaaaag tttctgaagc
    40261 tgataaaaca tttatgaaag caatggctaa gtttgaagga gtttcccttt cggaacttat
    40321 tcgcaccaaa actcttgaag ccctagaaga tgaatacgat gctcgtgtgg cagatttagc
    40381 ctatcaagag tatttagaag acttggaaaa aggagttgaa cccattactt gggaagaaat
    40441 gatgcatgat ttaggcttga aggatgaata atttgtataa attagttcca acaagacgtt
    40501 ttatcaagca attgaaaaaa ttggaccgtt atacgcagaa gctaattaca aactatttac
    40561 aaaccaatgt tttggaagac ccaagacgac acggaaaggc tttggttggt aatcgcgttg
    40621 gtcaatggcg ctatagaatt ggtaattatc gagttatcgt acaaattgta gatgatgaat
    40681 tagtcgttgc tactctagaa gttggtcatc ggagagatat ttattgaatt acttttttca
    40741 agtaaatttt cattctttaa aatatgatat aatagggctt acaatataag agaggttgct
    40801 gatgacaata gatattagtg aagaaagttt ggctaaagaa tcggctgact tattaaaaat
    40861 tcttttaaaa gatcgaacga caaagaaatc aattgtttgg gctacgcatt cttatgaatt
    40921 gttaggaaag ggatttgctc caagtgatcg catcaatcca agtaaggtta ctggaaactt
    40981 tgccaacttg attcagccca gatctgaaaa atccaaatat gaacaaaaag atcggacaaa
    41041 aataagggca gaagtgttta caccaacgtg gttggtcgca aagcaaaatg gatatgtaga
    41101 atcgaaatta ggctcgttaa gtcttgagga gtatgtggac ttacgatggt tagaggtaac
    41161 ttgtggtgaa gcaccttata tggtaacgag atatgatacc gtaactggtg aggaaattcc
    41221 attatctgag cgagttggtt ttgttgatag aaaactgcaa cggattagtc gtgaggtttc
    41281 agatgaagtc acattttata aacttgtaaa agatgcttat catgcttcgt atggttatga
    41341 gtatcaagga gattctctcc ttttggcgcg tgagaatctt ttggcaactt ttgaggacta
    41401 ttatctagct aagactggaa atcaaccaac attagagcaa aagaaagaaa ttgcgactat
    41461 tatctcttac aatgtctttc agatggatgg attgaaaaaa agtagtcctt attcagctaa
    41521 acaaaagcaa tctcaacaat tgagtttgtt tgcggatgag ctagaagtcc aagaagtaga
    41581 agaatctaaa acccatatca aagactggaa aaagaataga atgataggtt ttgagcgcct
    41641 atctagcgag gaaagtgaaa tgaaatttga tgtcgtgata ggaaatcctc cgtatcagga
    41701 aacaggagaa gccagagatg agccgatata tcactatttt attgatgaag cttataaaat
    41761 cgcagataaa tctatactaa tcaccccagc aaggttttta tttaaagctg gtcaaactcc
    41821 taaaaattgg atggaaaaga tgcttgaaga caagcattta aaagttcaat tctatgaact
    41881 taaaagcggt aaagtattta caggaacgga tattaagggt ggagtagcaa taacttatcg
    41941 tgatgttgat aaagatttgg gaccaatcgg agtatttact atttttgaaa ctttgaatgg
    42001 tattgtaaaa aaaaatcgaa aaacatccag aatttgaaac tttttctgat ttagtttatc
    42061 ctcaaggtat ttatagattt tcaaataaac ttttttctga ttatccaaat gctgaaaagc
    42121 tgcaaggaaa agggacaaaa aataaaattg tttcgaaatc actaactcag ttagattttg
    42181 ttttcaaaaa caacgaagaa aaagaccatg ttgctctact tggaagaatt ggctccgaac
    42241 gtgtttatcg atatattaat aaaaaatatt tagatttacc ggaaacattc gaaaattata
    42301 atgtttttgt accagaagct aatggaagtg gtgccttagg tgaagtctta tcaacacccc
    42361 taatcgggga acccctaatc gggcatacag atactttttt atctattggt aattttaaaa
    42421 caaaatttga agccgatgct tgtattaaat ttattaaaac taaattcgct agagtattat
    42481 taggtgtttt gaaagttact cagcataatt cacgcaaaac ttggtattac gtccccctcc
    42541 aagactttac ggtcaattcg gacattgatt ggacacaatc agtgactgat attgaccgcc
    42601 agcttgatca aaaatatgac ctttcccctg aagaaattgc ctttattgag aatcatgtaa
    42661 gggagatgga ttagaaaagt atttttattt gacaaatagt gctcaatgat ctaaaatgac
    42721 tatataggat taggtcagga agcatacgat gccctgaccc tttttgtact tatgagatga
    42781 gaaagtcatt tgttagataa attgacttcg ttagcaaacg ttcaaaaaag gaaaacttat
    42841 gccagtagaa attaaaacca ctaaagaaat tcatcctaaa atctatgcct acaccacacc
    42901 gacagtaacc agtaatgaag gctggattaa gattgggtat acagaacgtg atgtcacaca
    42961 acgtatcaag gagcaaacgc atacagctca tatagctaca gatgtcttat ggactggtga
    43021 tgcagcttat acagaagagc ctgataaggg gaaaactttc aaggaccatg atttccacca
    43081 tttcctttct ttccatgatg tagaacgtcg tcccaagacg gaatggttct attttaatgg
    43141 aactcctgaa aaatcaaaaa atctttttga taagtttgtt cagcatgatt tgtctggtta
    43201 tcagcctgga aaaggacagg actatactct gcgacaagag caagaagaag cagttgctaa
    43261 gacattagct tatttccaag aacatgctgg aggcaagttt ctctggaatg ccaagccacg
    43321 ctttggtaaa accttgtcta cctatgacct agctcgacgg atggaagctg tcaatgtcct
    43381 aattgtaaca aaccgccctg ccattgctaa ctcatggtat gatgattttg aaacattcat
    43441 agcaggtcaa acgacttaca agtttgtttc tgaatcagat agccttaaga gtcgtccaat
    43501 cttgtcacga caagaatttc ttggtatttt agctgacgat gtaagacaac ttgcttttat
    43561 cagtctccaa gacttgaaag gatctgttta tttaggtgga gagcacgata aactcaaatg
    43621 ggtaactgat ctgcattggg acttgttggt tattgacgag gctcatgaag gagttgatac
    43681 cttcaagact gaccaagcct ttaataagat tcgacgaaat tttactctgc atttgtcagg
    43741 tacatcattt aaagcattgg ctaaaggaga ttttacagag gaacaaatct acaactggtc
    43801 ttatgctgat gagcaggctg ctaagtattc gtggtctctt gagcaagaag aggaaaatcc
    43861 ttatgaaagc ttgcctcagt tgaatctctt tacctatcaa atgtctcaga tgattggcga
    43921 aaagttagaa aaaggcgctc agatcgatgg tgaaaatatt gactatgttt ttgacttaag
    43981 tgaatttttc gctacagatg ataaagggaa atttattcat gagcatgatg tcagaaattg
    44041 gttagatact ctatcaagca atgaaaaata tccattttca accaaagaac tccgtaatga
    44101 actcaagcat actttttggc ttttagaacg tgtcgcttcg gccaaagcat taaaagccct
    44161 actagaagaa cacccaatct atgaaaacta tgagatcgtt ctagctgctg gtgacggacg
    44221 tatgtccgaa gaagacgata aagtcaaact caaatccttg gacttggtta gaaaagcgat
    44281 agcagagaat gacaaaacca ttaccctatc cgttggtcag ctgacgacag gtgtcactat
    44341 ccctgaatgg acaggtgtat tgatgttatc aaatttgaaa tcaccagctc tttatatgca
    44401 ggccgccttc cgtgctcaaa atccttactc atggagcgat aacaaaggaa atcactttcg
    44461 caaagaaaga gcctatgtat ttgactttgc gccggaaaga accttgattc tctttgatga
    44521 gtttgccaac aacttattgc ttgtaactgc agctggtaga ggaacttcag ctacacgcga
    44581 agaaaatatt agagaattat taaacttctt tccaattatt gccgaagacc gtgctggtaa
    44641 gatggttgaa attgatgcaa aggcagttct aaccactcct cgccagataa aagctagaga
    44701 agttcttaaa cgaggtttta tgtccaatct cttatttgat aatattagtg gtattttcca
    44761 agcaagtcaa acagttttag atattttaaa tgagctgcca gttgaaaagg aagggaaggt
    44821 acaagatagt tctgatttat tagatttttc agatgttaca gtcgatgatg agggaaatgc
    44881 agtagtagac catgaaattg tagttaatca gcaaatgcga ctttttggtg aaaaagttta
    44941 tggacttggt gaatctgttg ctgagttagt cacaaaagat gaggaacgaa ctcaaaaaca
    45001 gctggtcaat gacttgagta agacaagacc gtttcttcag tgattgtaga ggaattgaaa
    45061 gcagattatt ctctaaaaac aagggaaact gagcaaatta agaaacaaat tacagcaaca
    45121 cttgagaatg aaattcgaaa aaatgatatc gaaagaaaaa tttctgaagc tcatatcaag
    45181 caagagttgc aacagcagct caaagaagca aatgataaag cgcaaaaaga taagattcaa
    45241 gaagatttgg aaaaacgttt agaagaaaat aaactcattc ataaagaaaa actagaacaa
    45301 acactcaaaa aagaagtgga aaaaatgcct gagaaattta tcgaacaggt tgagataaaa
    45361 cgtgtggaac agttgaaaca atcagctcaa gatgaaattc gtgaccattt acgagggttt
    45421 gcaagaacaa ttccaagttt tattatggct tacggtgatc aaactctaac acttgataat
    45481 tttgatgcct ttgttcctga acatgttttt tatgaagtaa cagggattac gattgatcag
    45541 tttagatatt tgcgagatgg tgggcaggat tttgcagggc atctctttga taaagcaaca
    45601 tttgacgaag ctattcaaga atttcttcgc aagaaaaagg agttggcgga ttattttaaa
    45661 gatcaaaaag aagacatttt tgactatatt ccaccgcaga agaccaacca aattttcact
    45721 cctaaacgag tggtgaaaag gatggtagat gatttggaaa aggaaaatcc agggattttt
    45781 gatgatccat ctaagacttt tattgattta tatatgaagt caggcctcta tattgcagaa
    45841 cttgtgaagc ggttatataa tagcaatggc ttgaaagagg cctttccaaa tcctgaagaa
    45901 cgcttaaaac atattttgga aaagcaagtt tatggatttg ctccgtctga gattatctat
    45961 aacatttcca ctaattttat atttggcaat ctttctaaag atatcagtag gaagaatttt
    46021 gttttagcag ataccattcc agcggctaaa gaagggagca ttcaaaagtt ggttgattcc
    46081 tattttgaaa ataattaaaa agaaggccga gtcaaaattc tttgaaatca gaaaaaacgc
    46141 ataatattga gtgcttttgt actgcccccc aaaagttaga cagaaaaaat ctaacttttg
    46201 gggggcagtt cagacaatcc ttggtattat gcgttttatt gtgggaagat gtataatgga
    46261 ttgaaataag atatgaacaa atcaattagg aatttaaagc attttataac aacgttttag
    46321 agtaatgggg ggctatttca acttcaacct actataatac agaaaaaaac aactccctga
    46381 taattcaagg agttgtctat agttaaatta gtttttagaa gcttcttgga attctgggtt
    46441 tttccatgct tcgtcaatga tagcttgtaa ttctttagca gatgcttgca ttttttgagt
    46501 ttctgcgtcg ttcaatggga tatttactgg acgaacgata ccatgtgcac caacaacagc
    46561 tggttgaccg ataaagacat tctcaactcc gtattgacct tcttggaata ctgaaagtgg
    46621 aagtactgcg ttttcatcgt caaggattgc tttagtgata cgagcaaggg ctactgcgat
    46681 accgtagtat gttgcacctt ttttgttgat gattgtgtag gctgcatcac gaacaccttc
    46741 gaacaattca atcaattcag cttcttgaac attttgagtg tctttaagga attcttcaag
    46801 gtttacacca gcgatgttag cgtgtgacca aacagcgaac tcagagtcac cgtgttcacc
    46861 catgatgtag gcgtgcactg aacgagcatc cacatccaat ttttcagcaa gtgcttgacg
    46921 gaaacgagct gagtcaagtg aagtacctga accgataacg cgttctttag ggaaaccaga
    46981 gaatttccaa gttgagtaag tcaaaacgtc aactgggtta gcagcaacaa ggaagatacc
    47041 tttgaaacca gattcaacaa cttgagttac gattgatttg ttgatagcaa ggtttttacc
    47101 tacaaggtca agacgagttt cacctggttt ttgaggtgca cctgcagtga tcacaacaag
    47161 gtcagcgtct gcacagtcag agtattgagc tgcatagatt tttttaggtg aagtgaaggc
    47221 aagggcgtga ctaaggtcaa gcgcatcacc aacagctttt tcatgcaatt gtggaatttc
    47281 gataattcca agctcttgtg caattccttg gttaacaagt gcaaaagcgt aagatgaacc
    47341 tacagcacca tcaccgacaa ggataacttt tttgtgttgt ttagttgaag tcattgtttt
    47401 aaacatctcc ttaattttat taggggattt tccctagaca acttcattct atcactttta
    47461 aaaaactttg tcacgaatat gccttatagt tctcgatgta aacgttttag tggtttagag
    47521 gctgaaatag atgggaattt atggtataat gttgttactt actaattgtg aaatgaggca
    47581 tttattaatg caggataaaa atttagtgaa tgtcaatctg acaaaggaga tgaaggcaag
    47641 ttttatcgac tacgccatga gtgttatcgt agcgcgagct cttcctgatg ttcgagatgg
    47701 cttaaaacct gttcaccgtc gcattctcta cggaatgaat gaattgggtg tgaccccaga
    47761 caaaccccat aaaaaatctg ctcgtattac aggggatgtc atgggtaaat atcacccaca
    47821 cggggattcc tctatttatg aagccatggt ccgtatggct caatggtgga gctaccgtta
    47881 catgcttgta gatggtcatg ggaattttgg ttccatggat ggagatagtg ctgccgctca
    47941 acgttatacc gaggcacgta tgagcaagat tgctctggaa atgcttcgtg atatcaacaa
    48001 aaatacagtt gatttcgttg ataactatga tgccaatgaa cgggaaccct tggtcttgcc
    48061 agcgcgtttt ccaaaccttt tggttaatgg agcaactggt atcgcggttg ggatggcaac
    48121 caatattcca cctcataatc tgggtgaaac cattgatgca gtgaagttgg tcatggataa
    48181 tcctgaagtg actaccaagg acttgatgga agtcttgcct ggaccagatt ttccaactgg
    48241 tgctcttgtc atggggaaat caggtatcca taaggcttat gaaacaggta aaggttcgat
    48301 tgtcctacgt tctcgtacag agattgaaac gactaagact ggtcgtgagc gtatcgttgt
    48361 aacagaattt ccttacatgg tcaataaaac caaggtgcat gagcatattg ttcgcttggt
    48421 tcaggaaaaa cgcattgagg gtatcacagc agtacgtgat gagtcaaacc gtgaaggtgt
    48481 tcgatttgtt attgaagtca agcgcgacgc ctcagccaat gttattctca ataacctctt
    48541 caaaatgacc caaatgcaaa ccaattttgg tttcaatatg ctcgctatcc aaaatggtat
    48601 accgaaaatt ttgtctcttc gtcagatttt ggatgcttat atcgagcacc aaaaagaagt
    48661 ggttgttcgt cgtacacgtt ttgataagga aaaagcggaa gcgcgcgctc atatcttaga
    48721 aggtctcttg attgcgctag accatatcga cgaagtgatt cgtatcatcc gtgctagtga
    48781 aacggatgcg gaagctcaag ctgagttgat gagcaagttt aagctttctg aacgtcaaag
    48841 tcaagctatc cttgatatgc gtcttcgtcg tttgacaggt ttggaacgcg ataagattca
    48901 atctgagtat gatgacctct tggctctgat tgcggattta gcagatattc ttgctaagcc
    48961 tgaacgtgtt tctcaaatta tcaaagacga attggatgaa gttaaacgta aattttctga
    49021 taagcgccgt acagagttga tgattggaca ggtcttgagt ctcgaggatg aggacttgat
    49081 tgaagaatcg gatgtcttga ttaccctttc taacagaggc tacattaagc gtttggatca
    49141 ggacgagttc actgctcaaa aacgtggggg tcgtggtgtc caaggaacgg gagtgaaaga
    49201 tgatgacttt gttcgtgagt tagtgtcaac tagcacccat gatcatctgc tcttcttcac
    49261 aaacaaggga cgtgtctatc gtcttaaagg ttatgaaatt cctgagtatg gtcggactgc
    49321 caaagggcta ccagtagtca atctcttgaa attggatgaa gacgaaagta ttcagacggt
    49381 tatcaatgtt gagtctgatc gcagtgatga tgcttatctc ttctttacaa cccgtcacgg
    49441 tattgtgaag agaaccagtg ttaaggagtt tgccaatatt cgtcaaaatg gtctcaaagc
    49501 gctgaattta aaggatgaag atgagttaat caatgtcttg ttggcagaag gagatatgga
    49561 tattatcatt ggtaccaagt ttggttatgc agttcgcttt aatcaatcag ccgttcgtgg
    49621 tatgagccgt atcgccactg gtgtgaaagg tgttaacctt cgtgaaggag acacagttgt
    49681 tggtgccagc ttgattactg atcaagatga ggttcttatt atcacagaaa aaggatatgg
    49741 taagcgtaca gtcgctactg aatacccaac aaaaggtcgt ggtggtaagg gaatgcagac
    49801 agctaaaatt accgaaaaaa atggcttgct ggccggtctt atgactgttc aaggggatga
    49861 ggatttgatg attatcactg atacaggtgt catgattcga accaatcttg ccaatatttc
    49921 acaaacagga cgtgcaacta tgggagttaa agtaatgcgc ctggatcaag atgctcagat
    49981 agtgactttc acaacggttg cggtggcaga aaaagaagaa gttgggacag aaaacgaaac
    50041 agaaggtgaa gcataatgtc tcaaaaaaat aataaaaaga aaaacaagcg aaaaaatctg
    50101 ctgacaaata tcctagcagg atttctgata ttactgtcac tggctttgat ttttaatact
    50161 caaattcgaa atattttcat agtctggaat accaataagt atcaagttag ccaggtatca
    50221 aaagaaaaat tagaagaaaa tcaggataca gaaggcaatt ttgactttga ttctgtcaaa
    50281 gctatctctt cggaagctgt tctaacttct caatgggatg ctcaaaaatt accagttatt
    50341 gggggaattg caattcctga attggaaatg aatttgccga tttttaaagg acttgataat
    50401 gttaatctct tctacggagc tggtacaatg aaacgcgagc aagtaatggg agaaggaaat
    50461 tatagtctag ctagtcacca tatctttggt gttgataatg ctaataaaat gttattttct
    50521 cctttagata atgctaaaaa tggcatgaag atttatctaa ccgataaaaa taaagtttat
    50581 acttatgaaa tacgtgaagt caaacgtgtg acaccggatc gtgttgatga agttgatgat
    50641 agagatgggg tcaatgaaat cacattagta acctgtgaag accttgctgc tacagaacgt
    50701 attattgtca aaggtgattt gaaagaaaca aaagattatt cacaaacatc taatgaaatc
    50761 ctaacagctt tcaatcaacc atataaacaa ttttattaat acaaatcagt gaaatccatg
    50821 atttcactga ttttttaaga ttttca
//
LOCUS       DAVKNL010000008        50687 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVKNL010000008 DAVKNL010000000
VERSION     DAVKNL010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 50687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50687
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.008"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            <1..886
                     /locus_tag="VW411_000406"
     CDS             <1..886
                     /locus_tag="VW411_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9793200.1"
                     /translation="VFISVMASSISYVIEDVLIPQVVEYDKIVFANSLFSISYKVLDS
                     IFNSFASFLQVAVGFILLVKIDIGIFLLALFILLLLKFRTSNANIENFSFKYYKREVL
                     QGTKFILNNKLLFKTSISLTLINFFYSFQTVVVPIFSIRYFDGPIFYGIFLTIAGLGG
                     ILGNMLAPIVIKYLKSNQIVGVFLFLNGSSWLVAIVIKDYTLSLILFFVCFMSKGVFN
                     IIFNSLYQQIPPHQLLGRVNTTIDSIISFGMPIGSLVAGTLIDLNIELVLIAISIPYF
                     LFSYIFYTDNGLKEFSIY"
     gene            1311..1544
                     /locus_tag="VW411_000407"
     CDS             1311..1544
                     /locus_tag="VW411_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9793201.1"
                     /translation="MISIDLGGTNIKITVLSNDGEIETLWSITTDTSEKGSQIISDII
                     SSIKNKLTERNIPDSDLLGIGMGSCSSYFPCKS"
     gene            1827..3776
                     /gene="mutL"
                     /locus_tag="VW411_000408"
     CDS             1827..3776
                     /gene="mutL"
                     /locus_tag="VW411_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEU9793202.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLISD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(4101..4568)
                     /locus_tag="VW411_000409"
     CDS             complement(4101..4568)
                     /locus_tag="VW411_000409"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEU9793203.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(4569..5774)
                     /locus_tag="VW411_000410"
     CDS             complement(4569..5774)
                     /locus_tag="VW411_000410"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEU9793204.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAKMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(5794..6429)
                     /gene="ribE"
                     /locus_tag="VW411_000411"
     CDS             complement(5794..6429)
                     /gene="ribE"
                     /locus_tag="VW411_000411"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEU9793205.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(6414..7514)
                     /gene="ribD"
                     /locus_tag="VW411_000412"
     CDS             complement(6414..7514)
                     /gene="ribD"
                     /locus_tag="VW411_000412"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEU9793206.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKVFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(7648..7762)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            7919..8512
                     /gene="ruvA"
                     /locus_tag="VW411_000413"
     CDS             7919..8512
                     /gene="ruvA"
                     /locus_tag="VW411_000413"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU9793207.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            8522..9085
                     /locus_tag="VW411_000414"
     CDS             8522..9085
                     /locus_tag="VW411_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEU9793208.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQVLFELLCMETYQA
                     GLSWETVLNKRQAFREVFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKLFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            9082..9759
                     /locus_tag="VW411_000415"
     CDS             9082..9759
                     /locus_tag="VW411_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9793209.1"
                     /translation="MMSNKNKEILIFAILYTVLFMFDGVKLLASLMPSAIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLSGLLSIILV
                     GLVFALTHMHSLALSEWIDAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(9896..10927)
                     /locus_tag="VW411_000416"
     CDS             complement(9896..10927)
                     /locus_tag="VW411_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEU9793210.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVILFDYNK"
     gene            complement(11182..11865)
                     /locus_tag="VW411_000417"
     CDS             complement(11182..11865)
                     /locus_tag="VW411_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEU9793211.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(11877..12821)
                     /locus_tag="VW411_000418"
     CDS             complement(11877..12821)
                     /locus_tag="VW411_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU9793212.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            12953..15784
                     /gene="uvrA"
                     /locus_tag="VW411_000419"
     CDS             12953..15784
                     /gene="uvrA"
                     /locus_tag="VW411_000419"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU9793213.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLREGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            15777..16838
                     /locus_tag="VW411_000420"
     CDS             15777..16838
                     /locus_tag="VW411_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU9793214.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            16970..17368
                     /gene="spx"
                     /locus_tag="VW411_000421"
     CDS             16970..17368
                     /gene="spx"
                     /locus_tag="VW411_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEU9793215.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            17434..18003
                     /locus_tag="VW411_000422"
     CDS             17434..18003
                     /locus_tag="VW411_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU9793216.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLAIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            18090..18356
                     /locus_tag="VW411_000423"
     CDS             18090..18356
                     /locus_tag="VW411_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEU9793217.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            18360..18779
                     /gene="ruvX"
                     /locus_tag="VW411_000424"
     CDS             18360..18779
                     /gene="ruvX"
                     /locus_tag="VW411_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEU9793218.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            18795..19100
                     /locus_tag="VW411_000425"
     CDS             18795..19100
                     /locus_tag="VW411_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEU9793219.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            19359..20609
                     /locus_tag="VW411_000426"
     CDS             19359..20609
                     /locus_tag="VW411_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU9793220.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLTDQVKE
                     MEKTLLQTPAQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGEFAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLACQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNLSQLNIGLEGVYQQENAALALQTFLLFMRERKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLAEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTEKLPQVELKVTGFDYQGALDERDVTGYDIVSSYREFISDFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            20606..21151
                     /locus_tag="VW411_000427"
     CDS             20606..21151
                     /locus_tag="VW411_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEU9793221.1"
                     /translation="MTPFFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDID
                     GTYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTLDSANQRMIIGDDVKIYTVN
                     GNQIVVDDMDRDPSDQIVLTK"
     gene            21297..22829
                     /gene="cls"
                     /locus_tag="VW411_000428"
     CDS             21297..22829
                     /gene="cls"
                     /locus_tag="VW411_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEU9793222.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            22907..23050
                     /locus_tag="VW411_000429"
     CDS             22907..23050
                     /locus_tag="VW411_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793223.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            23087..24625
                     /locus_tag="VW411_000430"
     CDS             23087..24625
                     /locus_tag="VW411_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU9793224.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            24745..26952
                     /gene="nrdD"
                     /locus_tag="VW411_000431"
     CDS             24745..26952
                     /gene="nrdD"
                     /locus_tag="VW411_000431"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEU9793225.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKCLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKQEDYAWK
                     KAQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            26964..27104
                     /locus_tag="VW411_000432"
     CDS             26964..27104
                     /locus_tag="VW411_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793226.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            27148..27654
                     /locus_tag="VW411_000433"
     CDS             27148..27654
                     /locus_tag="VW411_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9793227.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKFQSPHDGGFWDTENFVYEDWL
                     ESNQEQEMGINLPEGWVSAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            27647..28237
                     /gene="nrdG"
                     /locus_tag="VW411_000434"
     CDS             27647..28237
                     /gene="nrdG"
                     /locus_tag="VW411_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEU9793228.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLEFLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            28234..28851
                     /locus_tag="VW411_000435"
     CDS             28234..28851
                     /locus_tag="VW411_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEU9793229.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRHLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            29118..29426
                     /gene="rpsJ"
                     /locus_tag="VW411_000436"
     CDS             29118..29426
                     /gene="rpsJ"
                     /locus_tag="VW411_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEU9793230.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            29643..30269
                     /gene="rplC"
                     /locus_tag="VW411_000437"
     CDS             29643..30269
                     /gene="rplC"
                     /locus_tag="VW411_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEU9793231.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            30294..30917
                     /gene="rplD"
                     /locus_tag="VW411_000438"
     CDS             30294..30917
                     /gene="rplD"
                     /locus_tag="VW411_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEU9793232.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            30917..31213
                     /locus_tag="VW411_000439"
     CDS             30917..31213
                     /locus_tag="VW411_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEU9793233.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            31231..32064
                     /gene="rplB"
                     /locus_tag="VW411_000440"
     CDS             31231..32064
                     /gene="rplB"
                     /locus_tag="VW411_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU9793234.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            32168..32449
                     /gene="rpsS"
                     /locus_tag="VW411_000441"
     CDS             32168..32449
                     /gene="rpsS"
                     /locus_tag="VW411_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEU9793235.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            32461..32805
                     /gene="rplV"
                     /locus_tag="VW411_000442"
     CDS             32461..32805
                     /gene="rplV"
                     /locus_tag="VW411_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEU9793236.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            32818..33471
                     /gene="rpsC"
                     /locus_tag="VW411_000443"
     CDS             32818..33471
                     /gene="rpsC"
                     /locus_tag="VW411_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEU9793237.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            33475..33888
                     /gene="rplP"
                     /locus_tag="VW411_000444"
     CDS             33475..33888
                     /gene="rplP"
                     /locus_tag="VW411_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEU9793238.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            33898..34104
                     /locus_tag="VW411_000445"
     CDS             33898..34104
                     /locus_tag="VW411_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEU9793239.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            34129..34389
                     /gene="rpsQ"
                     /locus_tag="VW411_000446"
     CDS             34129..34389
                     /gene="rpsQ"
                     /locus_tag="VW411_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEU9793240.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            34415..34783
                     /gene="rplN"
                     /locus_tag="VW411_000447"
     CDS             34415..34783
                     /gene="rplN"
                     /locus_tag="VW411_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEU9793241.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            34861..35166
                     /gene="rplX"
                     /locus_tag="VW411_000448"
     CDS             34861..35166
                     /gene="rplX"
                     /locus_tag="VW411_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEU9793242.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            35190..35732
                     /gene="rplE"
                     /locus_tag="VW411_000449"
     CDS             35190..35732
                     /gene="rplE"
                     /locus_tag="VW411_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEU9793243.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            35750..36019
                     /gene="rpsN"
                     /locus_tag="VW411_000450"
     CDS             35750..36019
                     /gene="rpsN"
                     /locus_tag="VW411_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEU9793244.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            36304..36702
                     /gene="rpsH"
                     /locus_tag="VW411_000451"
     CDS             36304..36702
                     /gene="rpsH"
                     /locus_tag="VW411_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEU9793245.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            36894..37430
                     /gene="rplF"
                     /locus_tag="VW411_000452"
     CDS             36894..37430
                     /gene="rplF"
                     /locus_tag="VW411_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEU9793246.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGSKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            37514..37870
                     /gene="rplR"
                     /locus_tag="VW411_000453"
     CDS             37514..37870
                     /gene="rplR"
                     /locus_tag="VW411_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEU9793247.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            37888..38382
                     /gene="rpsE"
                     /locus_tag="VW411_000454"
     CDS             37888..38382
                     /gene="rpsE"
                     /locus_tag="VW411_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEU9793248.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            38354..38578
                     /gene="rpmD"
                     /locus_tag="VW411_000455"
     CDS             38354..38578
                     /gene="rpmD"
                     /locus_tag="VW411_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEU9793249.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            38723..39163
                     /gene="rplO"
                     /locus_tag="VW411_000456"
     CDS             38723..39163
                     /gene="rplO"
                     /locus_tag="VW411_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEU9793250.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            39176..40486
                     /gene="secY"
                     /locus_tag="VW411_000457"
     CDS             39176..40486
                     /gene="secY"
                     /locus_tag="VW411_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEU9793251.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            40637..41275
                     /locus_tag="VW411_000458"
     CDS             40637..41275
                     /locus_tag="VW411_000458"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEU9793252.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGVINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            41392..41610
                     /gene="infA"
                     /locus_tag="VW411_000459"
     CDS             41392..41610
                     /gene="infA"
                     /locus_tag="VW411_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU9793253.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            41635..41751
                     /gene="rpmJ"
                     /locus_tag="VW411_000460"
     CDS             41635..41751
                     /gene="rpmJ"
                     /locus_tag="VW411_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEU9793254.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            41769..42134
                     /gene="rpsM"
                     /locus_tag="VW411_000461"
     CDS             41769..42134
                     /gene="rpsM"
                     /locus_tag="VW411_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEU9793255.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            42152..42535
                     /gene="rpsK"
                     /locus_tag="VW411_000462"
     CDS             42152..42535
                     /gene="rpsK"
                     /locus_tag="VW411_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEU9793256.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            42578..43513
                     /locus_tag="VW411_000463"
     CDS             42578..43513
                     /locus_tag="VW411_000463"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU9793257.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            43525..43911
                     /gene="rplQ"
                     /locus_tag="VW411_000464"
     CDS             43525..43911
                     /gene="rplQ"
                     /locus_tag="VW411_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU9793258.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTESRRGDAAPMAIIELV"
     gene            44176..44442
                     /locus_tag="VW411_000465"
     CDS             44176..44442
                     /locus_tag="VW411_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEU9793259.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            44452..45789
                     /locus_tag="VW411_000466"
     CDS             44452..45789
                     /locus_tag="VW411_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEU9793260.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGKEGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            46033..46725
                     /locus_tag="VW411_000467"
     CDS             46033..46725
                     /locus_tag="VW411_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9793261.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGEIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(46828..48473)
                     /locus_tag="VW411_000468"
                     /pseudo
     CDS             complement(46828..48473)
                     /locus_tag="VW411_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(48463..49554)
                     /locus_tag="VW411_000469"
     CDS             complement(48463..49554)
                     /locus_tag="VW411_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9793262.1"
                     /translation="MSEIKIINAKKIYHDVPVIENLNITIPKGSLFTLLGASGCGKTT
                     LLRMIAGFNSIEGGEFYFDDTKINNMEPSKRNIGMVFQNYAIFPHLTVRDNVAFGLMQ
                     KKVPKEELIQQTNKYLELMQIAQYADRKPDKLSGGQQQRVTLACALAVNPSVLLMDEP
                     LSNLEAKLRLDMRQAIREIQHEVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRP
                     KELYHKPANEFVATFIGRTNIIPANLEKRSDGAYIVFSDGYALRMPALDQVEEQAIHV
                     SIRPEEFIKDESGDIEGTIRDSVYLGLNTDYFIETGFASKIQVSEESTFEEDLQKGNR
                     IRLRINTQKLNIFSADGSQNLIKGVNHGT"
     gene            complement(49567..50583)
                     /locus_tag="VW411_000470"
     CDS             complement(49567..50583)
                     /locus_tag="VW411_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9793263.1"
                     /translation="MKKKWMYYAACSSNESADDSSSDKGDGGSLVVYSPNSEGLIGAT
                     IPAFEEKYGIKVELIQAGTGELFKKLESEKEVPVADVIFGGSYTQYTTHGELFENYTS
                     KENDNVIKEYQNTTGYSTPYTLDGSVLIVNPDLTKGMNIEGYNDLFKPELKGKIATAD
                     PANSSSAFAQLTNMLQAQGGYKDDKAWSYVKDLFTLIDGKIGSSSSSVYKVVADGEMA
                     VGLSYEDPAVKLLNDGANIKVVYPKEGTVFLPASAAIVKKSKNMENAKKFIDFIISQE
                     VQDTLGTTTTNRPVRKNAKTSENMKPIDKIKTLTEDYDYVIKNKSDIVKKYNEVFTDI
                     QSKQ"
BASE COUNT        15278 a         9481 c        11064 g        14864 t
ORIGIN      
        1 agtgtttatt tcagtaatgg ctagctccat aagttacgtg atagaagatg tgttgattcc
       61 tcaagtggta gaatatgata agattgtatt tgcaaattct ctttttagta tttcgtataa
      121 agtattagat tctattttta attcattcgc atcattttta caggtggcag taggatttat
      181 tttattggtt aagatagata taggcatatt tttacttgct ctatttatat tgttgttgtt
      241 aaaatttaga actagcaatg cgaatataga aaacttctct ttcaaatatt acaagagaga
      301 agtgttgcaa ggtacaaagt ttattttaaa taataaatta ttatttaaaa ccagtatttc
      361 tttaacgctt ataaactttt tttattcatt tcagacagta gttgtaccga ttttttctat
      421 tcgatatttt gatggtccga ttttttatgg tattttttta actattgctg gtttgggtgg
      481 tatattggga aatatgctag cgccaatcgt aataaaatat ttaaaatcga atcaaattgt
      541 tggtgtattt ctttttttga acggctcaag ttggttagta gcaattgtta taaaagacta
      601 tactttatca cttattttat ttttcgtttg ttttatgtct aaaggagtct tcaatattat
      661 ttttaattcg ttgtaccaac aaatacctcc acatcaactt cttggtaggg taaatactac
      721 cattgattct attatttctt ttggaatgcc aattggtagt ttagttgcag gaacgcttat
      781 tgatttgaat attgaattag tgttaattgc tattagcata ccttattttt tgttttctta
      841 tattttttat acggataatg gattgaaaga atttagtata tattagaaat gtttatgttc
      901 attcaaaagc ataatgacta taactgaaaa agaaaagtga tatctttaag gttgttcttc
      961 ttggtggtga gattcgtgag acaacccaag cttttgtcgg aaagattacc aatgctttga
     1021 tggataggat gtactttagc aagatgtttt tagtggtaac ggtatcgtgg atggacgtgt
     1081 aataacctct tctttcgagg agtattttac taaaaaacta gccttggagc gttccccaga
     1141 aacggactta ctcattgact cttcaaagat ttggggagaa gattttgctt catctgttcc
     1201 ttgaaaaaag tcacagcagt catcacagac gatagtactg aacaaaacta tgaagagtta
     1261 gaaatttata cgcaggtgat tgtataaagg atctggaaat agataagaag ttgattagta
     1321 ttgacctagg tggtacaaat attaagatta ctgttctttc aaatgacggt gagattgaaa
     1381 ctttgtggag tattacaaca gatacaagtg agaaaggttc tcaaattata tcggacatca
     1441 tcagttctat taaaaataaa ttgaccgaac ggaatattcc tgatagcgac cttcttggaa
     1501 tcggtatggg aagttgctca tcatactttc cttgtaaatc ataggggcta taaactctcc
     1561 gtctacttgt cctgcaacaa ttgaagtctg ctcaaaacgc cgtccgctaa tcttttcata
     1621 gactttctcc cttttaggag cctagctttc tagtttgttc tttgattttt attgagtata
     1681 ccactatttt actccctctg gcaagggact ttgtctatgt ggagggattg ggctcctatg
     1741 tggtggagct tttctgttct ttctgaaata tggtataata gcactaatca atttctagga
     1801 aaatagatac agaaaggggc tgaaagatgt ctcatattat tgaattgcca gagatgctgg
     1861 caaaccaaat cgcggctgga gaggtcattg aacgtcctgc cagtgtggtc aaagagttgg
     1921 tagaaaatgc cattgacgcg ggctctagtc agattatcat tgagattgag gaagctggtc
     1981 tcaagaaggt tcaaatcacg gataacggtc atggaattgc ccacgatgag gtggagttgg
     2041 ccctgcgtcg ccatgcgacc agtaagataa aaaatcaagc agatctcttt cggattcgga
     2101 cgcttggttt tcgtggtgaa gccttgcctt ctattgcgtc tgttagtgtc ttgactctgt
     2161 taacggcggt ggatggtgct agtcatggaa ccaagttagt cgcgcgtggg ggtgaagttg
     2221 aggaagtcat cccagcgact agtcctgtgg gaaccaaggt ttgtgtggag gatctctttt
     2281 tcaacacgcc tgcccgtctc aagtatatga agagccagca agcggagttg tctcatatca
     2341 ttgatattgt caaccgtctg ggcttggccc atcctgagat ttcttttagc ttgattagtg
     2401 atggcaagga aatgacgcgg acagcaggga ctggtcaatt gcgccaagca atcgcaggga
     2461 tttacggttt ggtcagtgcc aagaagatga ttgaaattga gaactctgac ctagatttcg
     2521 aaatttcagg ttttgtgtcc ttgcctgagt tgactcgggc taaccgcaat tatatcagcc
     2581 tcttcatcaa tggccgttat attaagaact tcctgctcaa tcgtgctatt ttggatggtt
     2641 ttggaagcaa gcttatggtt ggacgttttc cactggctgt cattcacatc catatcgacc
     2701 cttatctagc ggatgtcaat gtgcatccaa ctaagcaaga ggtgcggatt tccaaggaaa
     2761 aagaactgat gactctggtt tcagaagcta ttgcaaatag tctcaaggaa caaaccttga
     2821 tttcagatgc cttggaaaat cttgccaaat cgaccgtgcg caatcgtgag aaggtggagc
     2881 aaactattct cccactcaaa gaaaatacgc tctactatga gaaaactgag ccgtcaagac
     2941 ctagtcaaac tgaagtagct gattatcagg tagaattgac tgatgaaggg caggatttga
     3001 ccctgtttgc caaggaaacc ttggaccgat tgaccaagcc agcaaaactg cattttgcag
     3061 agagaaagcc tgctaactac gaccagctag accatccaga gttagatctt gctagcatcg
     3121 ataaggctta tgacaaactg gagcgagaag aagcatccag cttcccagag ttggagtttt
     3181 tcggacaaat gcacgggact tatctctttg cccaagggcg agatggactt tacatcatag
     3241 atcagcacgc tgctcaggaa cgggtcaagt acgaggagta ccgtgaaagc attggcaatg
     3301 ttgaccaaag ccagcagcaa ctcctagtgc cctatatctt tgaatttcct gcggatgatg
     3361 ccctgcgtct caaggaaaga atgcctctct tagaggaagt gggcgtcttt ctagcagagt
     3421 acggagaaaa tcaatttatt ctacgtgaac atcctatttg gatggcagaa gaagagattg
     3481 aatcaggcat ctatgagatg tgcgacatgc tccttttgac caaggaagtt tctatcaaga
     3541 aataccgagc agagctggct atcatgatgt cttgcaagcg atctatcaag gccaatcatc
     3601 gtattgatga tcattcagct agacaactcc tctatcagct ttctcaatgt gacaatccct
     3661 ataactgtcc tcacggacgt cctgttttgg tgcattttac caagtcggat atggaaaaga
     3721 tgttccgacg tattcaggaa aatcacacca gtctccgtga gttggggaaa tattaaaagt
     3781 ataaaaaagt ctgggaaaaa ttttcaaaat caaaaaaacg cataaaatca ggtgttcaaa
     3841 aaccttgatt ttatgcgttt tatcatggaa atagttactt cattttttcc taattctttt
     3901 cgaaactctt tttaaacgac gtcagtttta tcagtaatct caaaacagtg ttttgagcta
     3961 attttgccag ttttgtctgt aacatcgaag ttgtgtttta ccactctgcg actggtttcc
     4021 tagtttgctc tatgattttc acagagcatt aaattgcgat tttgccaagt ttctttattc
     4081 gtctaaaagt agagtctgtt ctatgcgtct aatgtacgaa tcaggttgac catttcaata
     4141 gctccttgtg cacactcaga acccttattt cctgctttag taccagctcg ttctatggct
     4201 tgttcaattg tatctgtcgt tagcacacca aacataacag gaatttcgct atttaaactg
     4261 atttgggcga ttcccttaga tacctcgcta catacataat cataatgact tgtattccct
     4321 ctaatgacag ctcccaagca gataattgca tcatattttt tactttttgc catttttgat
     4381 gcaatcagtg gtatttcaaa agctcctgga acccaggcta cctctatatc tttctcgttt
     4441 acattctctc ttttgagatt atctagtgct ccagataata attttgaagt tataaattca
     4501 ttaaatctcg ctacaacaat acctatttta atattgtttg ctactaaatt accttcataa
     4561 gtgttcattt atttttcctc catatttaaa atgtgaccca ttcgattttt ctttgtttct
     4621 aaataaaaac tatcgtaagg attggcttct atttcgattg atattctact ggaaatggta
     4681 attccatatt tttctaactg ttcaaccttg tcaggattat ttgtcagtaa atgaagtgac
     4741 tgaagtccca gatctttaag catttttgct ccaatatgat attctcttaa atcaccttca
     4801 aagcctaatg caagattggc atcaagcgta tccatgcctt gatcttgtaa atgataggct
     4861 tttaatttat tgataagtcc aattcctcgt ccctcctgtc gcaagtaaag taagacaccc
     4921 gaaccattct caacaatcat tttcatagcc ttatcgaatt gctgtccaca atcgcaacgt
     4981 aaagagccta aaacatctcc tgttaaacat tcggagtgga cccgacataa tacattggct
     5041 tcatcctcta tatttcccat aataagagca agatgatgtt ccccatttag tttatctata
     5101 tagctaattg ctttgaaatt accgtatcta gtaggcatat tgacagttga aactcgttct
     5161 accagctgat catatacttt tctatattct tgtaattctt tgatggtaat tagtggaatg
     5221 ttgtgttttt tcgagaactg aattaaatca tctgttctca tcattttgcc atcatgattc
     5281 attatttcac aacataggcc acactctttt agtccagcta attttaataa atcaacagtt
     5341 gcttctgtgt gtccatttct ttctaggaca ccaccttttt ttgcaattaa aggaaacatg
     5401 tgtcctggcc tgcgaaaatc agagggtgtt atatcttcag ctacacacat acgtgcggtc
     5461 agtcctcttt cctcggcaga aatacctgtg gtcgtttctt tataatcaat tgaaactgta
     5521 aaagcagtct tatgattatc tgtattgttt tcaaccatag gtgaaagcat taattgatta
     5581 gctaaacttt cgctcatagg catacaaatt aatcctttgg cataagtagc cataaaatta
     5641 acattttctg ttgtagctgc ttgtgcagaa caaattaagt ctccttcatt ttctctatcc
     5701 ttgtcgtcta taacaagaac aagtcgtccc ttctgcaatg cttctaatgc ttcttgtatt
     5761 tttcgatatt ccattgactg attatccttt ctgctaaaat ccattttgat ataatagttc
     5821 cttagatatt tctgattttg gagagttatc catcagtttt tgcacatatt tacctaagat
     5881 atcattttca agatttactg tactcccgac ttgtttactc ttaagaatgg tttgttccaa
     5941 ggtatgaggg ataacagata ctgaaaagtt tactttggag actttagcga cagtcagact
     6001 aatgccgtca attgtaatag atcctttttc aactattaaa tctaaaattt ctttttgtgt
     6061 gttgatttga taccatacag cattatcatc tttttttatt gacgagattt ttcctgtacc
     6121 atcaatgtgt cctgtaacga cgtgaccccc aagtcgaccg ttgacagata aggctctttc
     6181 tagattcacc tcacttccat gttttaatag agtaagagct gttcgactcc atgtttcatt
     6241 cattacatca actgtaaagg attgatgatt gaaatgagta actgtaagac agataccatt
     6301 tactgctata ctatcgccta aatggatatc cgttaatatt tttgaggctt taattgatag
     6361 tttacaatta cgagagtctt tctgtattct ttcaactttt ccgatttctt caattattcc
     6421 tgtgaacatg gataaatcac ttcactttct atgagatagt catttcctat ttgagaaaat
     6481 gcataaggtt tcaatctaat agcgtcattt ggcaaagaaa taccttcacc tccgacagga
     6541 aacttggcac tacctccaaa aacttttggt gcaatatata ttttcagctc atcaacaatt
     6601 tgttgttcca aagcactcca attcattaga ctgccccctt ctagaactag gctatcaatc
     6661 tgcatgtttc ctagatgttg cattaaactc gataagtcta tatgattgcc ttttttcttt
     6721 atggaaagta tttcacagcc atgattttga tatagcttca ttttattttt gtcttcagag
     6781 gaagtggcaa tgtaagtttt aatatcattt gctgttttta cgattttaga ggtaagagga
     6841 gttcgtaaat gtgtatcgca tatgatacgg ataggatttt tcccttcctc caatctacat
     6901 gtcagcaaag gatcgtcttg aataacagta ttgactccca ccataattgc actaacatgg
     6961 tgtcgtaact gatgcacatg ctttcttgct tcttcttcag taatccattt ggattgattt
     7021 gttttagtgg ctatttttcc atccattgac attgcatatt tcataaaaac atagggtaca
     7081 tgctgggtaa tatactttct aaaacttttt attaagttaa gacactcatt ttctaaaatt
     7141 ccaacagtaa cttgaagatt attttcctca agtatcttta ctccttttcc agatacaata
     7201 ggattacagt ctaggcttcc aatgactact cttgtaatac cactatcgat tatagcatct
     7261 atacagggag gtgttttccc gaagtgacaa cagggttcaa gtgttacata aagcgtcgct
     7321 ccgacagggg attctctaca gtttttaaga gcatttctct cagcatgtgg gccaccaaaa
     7381 aactcatgat aaccttgtcc gataatgtga ttatctttta caataactgc gccgaccata
     7441 ggattgggat tgacgtaacc agcccctttt tgtgccagtt ttattgctaa tttcatatat
     7501 tttgaatcgc tcatctcgct acctccaaaa aaatatacct tgaatagggg actactcaag
     7561 gcatacaaaa gaaaacttat gcgattaaca aaaatgctct gaaatgacaa gtaatcattt
     7621 cagagcacgc aaaaagcaca aatatacttt tatcttcttt catccagact atactgtcgg
     7681 ctttggaatt tcaccaaatc atgcctttcg gctcgtgggc tataccaccg gtagggaatt
     7741 tcaccctgcc ctgaagatag ttattcaatt acagatgatt atagtactta attttgaata
     7801 tgtcaacaga taaataccga ttgtttttga tatactgtat ttgtgataat cgattctcgc
     7861 tcctcggata aagaaaatat gatatactag ataaacgaaa taagagagaa ggaatactat
     7921 gtacgcatat ttaaaaggaa tcattaccaa aattactgcc aaatacattg ttcttgaaac
     7981 caatggtatt ggttatatcc tgcatgtggc caatccttat gcctattcag gtcaggttaa
     8041 tcaggaggct cagatttatg tgcatcaggt tgtgcgtgag gacgcccatt tgctttatgg
     8101 atttcgctca gaggatgaga aaaagctctt tcttagtctg atttcggtct ctgggattgg
     8161 tcctgtatca gctcttgcta ttatcgctgc tgatgacaat gctggcttgg ttcaagccat
     8221 tgaaaccaag aacatcacct acttgaccaa gttccctaaa attggcaaga aaacagccca
     8281 gcagatggtg ctggacttgg aaggcaaggt agtagttgca ggagatgacc ttcctgccaa
     8341 ggtcgcagtg caagcaagtg ctgaaaacca agaattggaa gaagctatgg aagccatgtt
     8401 ggctctgggc tacaaggcaa cagagctcaa gaaaatcaag aaattctttg aaggaacgac
     8461 agatacagct gagaactata tcaagtcggc ccttaaaatg ttggtcaaat aggagcagag
     8521 aatgacaaaa cgttgttcgt gggtcaagat gaccaacccg ctctacatcg cctatcatga
     8581 tgaggagtgg ggccagcccc tccatgatga ccaagtattg tttgagttgt tgtgtatgga
     8641 aacctatcag gcaggcctgt cttgggaaac ggtactcaac aaacgccaag ctttccgaga
     8701 agtctttcat agctatcaaa ttcactcagt cgcagagatg actgacactg aattggaagc
     8761 catgctggag aatccagcta tcattcgaaa tagagccaag ctttttgcta cacgcgctaa
     8821 cgcccaagcc tttctacagt tacaggcaga gtacggctct tttgatgcct atctttggtc
     8881 ttttgttgag gggaaaactg tcgttaacga tgttcctgat tatcgccaag cgccagctaa
     8941 aacaccctta tctgagaaat tagccaaaga tctcaaaaaa cgaggcttca agttcacagg
     9001 cccagtcgcc gtattgtctt ttctacaggc tgcagggcta gttgatgacc acgagaatga
     9061 ttgtgagtgg aaaggtctta aatgatgtct aacaaaaata aggaaattct gatttttgcg
     9121 attctctata cagtcctctt tatgtttgat ggcgttaaat tgctggcttc tttaatgcca
     9181 tctgccattg caaattatct tgtttatgta gttttagctc tatatggctc cttcttgttc
     9241 aaggatagat tgatccaaca atggaaggag attagaaaga ctaaaagaaa attcttcttt
     9301 ggagtcttaa caggatggct ctttctcatt ctgatgactg ttgtctttga atttgtatca
     9361 gagatgttga agcagtttgt gggactagat ggacaaggtc taaatcagtc taatattcaa
     9421 agtacctttc aagaacaacc actactgata gctgtttttg cttgtgtcat tggacctctg
     9481 gtagaagaat tatttttccg tcaggtctta ttgcattact tgcaggaacg gttgtcaggt
     9541 ttactaagca ttattctggt aggacttgtt tttgctctga ctcatatgca cagtttggct
     9601 ctatcagagt ggattgatgc agttggttac ttaggtggag gccttgcctt ttctattatt
     9661 tatgtgaaag aaaaagagaa tatctactat cccctacttg ttcacatgtt aagcaacagc
     9721 ctctccttaa tcattttagc tatcagtata gtaaaatgaa atgagaacag gacaaatcga
     9781 tttctaacaa tgttttagaa gtagaggtgt actattctag tttcaatata ctgtaatatg
     9841 tgatgaaaat gccagtaatg ataccgagaa aaaagctgag aaacttttcc cagctttatt
     9901 tgttatagtc aaagagaatg acttgttcct gtgcatctac atgagcatgg accccaaagg
     9961 gtacaattgc tcttggagtt gcgtggccga cattcagatt atagacaatc gggatattgc
    10021 tgtcaatgat atccaatagt gcctctttat agtcgtcatg gaaagtttca tccataggtt
    10081 ttccgaccaa gagtccattg atgaccgcga atatgccagt gtcctttaaa gttagcaaca
    10141 tctttttgaa gtcttctggc ttaggctttt cttcgcttgt ttcgagcaag aggattttcc
    10201 cttcccagtc tgacaagtca gggaaaagtt tgtatttttg gcagagttcc gtgctatctg
    10261 cgtatcgaga gttgtcaaag atatcgtaga gggattcgag gcaaccaccg aggattttcc
    10321 cctcgaactg ggcacttcct tgcaacaagt caaaacctgt atttgtatga ctgacacgag
    10381 gtgttcccag ggccgtggga ctaaaatcag ttcgttcctc ataccaaacg tcactagggc
    10441 ggatttctga aattcttccc gtctcaatca attctttaaa gtagtgaagg ctataggcta
    10501 gcatttcttt gtctaattca caaatgtctg ctaaaaagga ttgaccataa aaagtcttga
    10561 ttcctaattt atgcaacatg aggtggttca tggttgtatc cgagaagcca agaaaaattt
    10621 tttgcttgat aaccttttgg agttggtcat tttcaaaaag ataaggtagc aagcgatagg
    10681 tatcgtctcc accgatggca cataggatca tgtcgatgct atcatcagaa aaggcatgaa
    10741 tcaaatcctc tgcacgagct tcaggatggt ccttgataaa gtctaatcct tttaacgaat
    10801 ggggcaaaaa gatgggattg agtcccagat ccttgagacg ttggataccc aagtccactt
    10861 cgtgtttgac aaaatcctct ccgataatgc cactagataa actaacaata ccaatagtag
    10921 aaaccatatc tcatcctcct agaaatagat tgagcctatt ttatcacaaa atataagaga
    10981 aagctatgtg ggatttcaga agaatacttt taatactctt cgaaaatctc ttcaaaccac
    11041 gtcagtttta tctgcaacct caaagctgtg ctttgagcaa cctgcagcta gcttcctagt
    11101 ttgctctttg attttcattg aatataactc aaaaaagtaa gtgaaaaagc aaccgcacct
    11161 gcagttgctt ttatcgtttt cttattcttg atagcgtgtt ggtcctgcac ttgcactacg
    11221 gatattcaag cgtttcttga gatagaagcg aagggctagg agggctgctc caataatagc
    11281 tagtggcaat ggatccagta ctgggttaag gctagctggt aggaagcttg ttgcaaagaa
    11341 gacaagcaac caaaggaaca tagaagctag gataactagt acagatttcc agaaaggtgg
    11401 acgttgactg cgatccatat ctggtccata gtattggtaa acaaagtagt acatcaagta
    11461 gaaggcaaat ccaccaacca gtccaactaa tagaagagta atcaatccat agccgaaagc
    11521 ttggtctgcc gcaaagaagg ttgtgagggc gctgacaagg gcaaagaggc tagtgatgaa
    11581 aagagctgag tccataatca tcagttttgg gtcatcattt tcttttggat gctctttttc
    11641 gtactgctct ttgacagtga agctatgagc ccaatgagtt ggtgcgccgt atagggaacg
    11701 ggcagttgta cctttagatt gctcctcaag gatttgggga atgacttcct caaaaatagt
    11761 ctggatttca gcgtctgttt tcccatcttt gatgaattgt tgggtagcaa tgtggacaaa
    11821 ctcttggttt ttcttagtta atttttgtag atcaatttga gacataggaa ctcctcttag
    11881 aaccattttt tatggatgag atagagagtg agcgagacac tcatagcaaa ggcgataaag
    11941 acgattaacc agaaggcatt tggctctccg tttaggggga tttcattatc cttaaagttc
    12001 atcccgtagg cagaaaagac catggttggg atggacatga cgatggtcac aagggccaag
    12061 gttttcatga tgttgttctg gttgttagaa atgatagagg caaaggtctc tgtcatagaa
    12121 tgcaagacgt ttccataaat atctgccatc tcgatggcct gttgggtttc aatcagggtg
    12181 tcttcaagca ggtcttcgtc ctcaaggtat ttcttgatat tgctggttga actggtcaat
    12241 ttcttaatca cgcgctcatt tgttttgagg gaggccttga aatagacgat agttttttcc
    12301 aattccatga gctcaatcaa ttcttcatta cgagttgatt gatgcagttg actttcgatt
    12361 tgttcactct tgcggtcgat tgaacgaagg gctgttaggt aaagctctgc attgcgataa
    12421 agaatttgaa agataaaacg tgaacgcatg aaggtataga aattacgcaa tcgacggttg
    12481 ataaagacat caaggacagg tagtggttcc aaacacgtag tgataatggt ttcctcagtg
    12541 atgataatac caagcgggat ggttacgtag taggtgcggt tatttctttc ctccgtgacc
    12601 ggcacgtcta cgataatcag ggtatactcg tcttcaatgg taatacgaga catttcttcc
    12661 gcatcgagcg gtgctcgaag gtcagcaata tcaatatcga aggtgttggc gatttcgagt
    12721 gattcatttt gagtcggatt gacgagattg atccaagtac ccggttcaag cgtatcgatc
    12781 tctttaaatt cagttgttgt agagagaaaa acttgtttca tatcccttat cctttctcat
    12841 ttttcagatt ttttcacact gtactattat actacaaaat cggccttttg ggtaatagaa
    12901 tctcactttt tttggtataa tggtaagcaa taatggacta gaaagaacaa agatgcaaga
    12961 taaaattgtc attcatgggg cgcgtgccca taatttaaaa aatattgatg tggagattcc
    13021 gcgagacaag ttggttgtcg tgactggctt gtcaggttca gggaagtcca gtctggcttt
    13081 tgatactctc tatgcggagg gacaacgtcg ctatgtagag agtttgtccg cctacgctcg
    13141 tcagttcttg ggaaatatgg aaaaaccaga tgtagatgcc attgatggtc tcagcccagc
    13201 tatttccatc gaccagaaaa cgactagtaa aaatcctcgc tcgacggtgg gaacgacgac
    13261 tgaaatcaat gactatctgc gtctcctcta cgcacgtgtg gggacgcctt actgtatcaa
    13321 cggacatgga gctatcaatg cttcttctgt ggagcaaatc gttgataagg ttttggagtt
    13381 acctgaacgc cagcgcttgc agatcttggc tcctgtcatc cgcaagaaaa aaggccaaca
    13441 taagagtgtt atcgagaagg ttcagaaaga cgggtatgtt cgtgtccgtg tggatgggga
    13501 agtctatgat gtgaccgaag tgccagagtt gtctaagagc aagcaacaca atatcgatgt
    13561 cgtggttgac cgtattgtta tcaaggaggg cattcgtagt cgtctctttg attccattga
    13621 ggctgccctt cgtattgcag aaggttatgt cattatcgac accatggacg actcggagtt
    13681 gttgttctct gagcattatg cctgtccagt ttgtggtttt actgtcccag agttagagcc
    13741 tcgtctcttt tctttcaatg ctccttttgg ctcttgtagt gagtgtgacg gcttgggcat
    13801 caagctggag gtggatactg atttggtagt gccagatgct agcaaaacct tacgtgaggg
    13861 agcgctagca ccttggaatc ctatctcatc caactactat ccaaacatgt tagagcaggc
    13921 catgaaagtc tttggagtgg ctatggataa gccttttgag gacctgtcag aagaagataa
    13981 gaacttgatt ctctatgggt cagatggtaa ggaattccat ttccactatg agaatgaatt
    14041 tggtggcgtg cgcgatatcg acattccgtt tgagggagtt atcaataata tcaaacgtcg
    14101 ttaccatgaa accaatagcg attacactcg cactcagatg cgtctctaca tgaatgagct
    14161 gacctgcgga acctgtcaag gctatcgtct caatgaccag gccttgtctg tccgtgtggg
    14221 cggccagcaa gggccacata ttggagaaat ctcagacctg tctatcgctg accacttgga
    14281 cttggtgagc cagttaactt tgtctgaaaa tgaagccatc attgctcgtc ccattctcaa
    14341 ggaaatcaag gatcgtttga ccttccttaa taatgtgggt cttaactatc taaccctgtc
    14401 acgttcagca ggaacccttt caggtgggga aagtcagcgc attcgtttgg caacccagat
    14461 tggttccaac ctatcaggtg tcctctatat cctagacgag ccgtcaatcg gtcttcacca
    14521 gagggacaat gatcgcctga ttgccagtct gaaaaagatg cgtgacttgg gcaatactct
    14581 tatcgtggtg gaacacgacg aagataccat gcgtgaggct gattatctga ttgacgttgg
    14641 tcctggtgcc ggtgtttttg gtggtgagat tgttgcagca ggtacaccta agcaagtggc
    14701 tcgcaacagc aagtctatca caggtcagta cttgtcaggc aaacgtgtca tcccagtacc
    14761 agaagagcgc cgtgtcggaa atggtcgttt tattgaagtg ataggagcgc gtgagaacaa
    14821 cttgcaaaat gtcacggctc gctttccact aggaaaattt atcgcagtga caggtgtgtc
    14881 gggttcaggg aaatcgactc taatcaacag cattctcaaa aaagccattg cccagaagct
    14941 caaccgtaat tcagacaaac ctggtaagtt taagactatt acagggattg agcatgtcga
    15001 ccgtttgatt gatattgacc agagccctat cggacggacg ccgaggtcta acccagctac
    15061 ctatacagga gtttttgacg atatacgtga cctttttgct cagacaaatg aagccaagat
    15121 tcgaggctac aagaaggggc gcttcagttt caacgtcaag ggaggtcgct gtgaagcttg
    15181 ctcaggtgac gggattatca agattgagat gcacttcttg ccagatgttt atgtggcttg
    15241 tgaagtttgc cacggaaccc gctataacag tgaaacccta gaagttcact acaaggaaaa
    15301 gaatatttcg caggtcttgg atatgacggt caacgatgcg gtagaatttt tccaacacat
    15361 tccgaaaatt caacgcaaac tccagaccat caaggatgta gggctaggct atgtgacctt
    15421 gggacagcca gctaccaccc tttctggggg agaagcccaa cgtatgaagt tagctagcga
    15481 actccacaaa cgatcgacag gaaaatcttt ctacattctg gatgagccga cgacagggct
    15541 tcatacagag gacattgctc gcctgctcaa ggttttagct cgctttgtag acgatggcaa
    15601 tacagtcctc gtcatcgagc acaatctgga tgttatcaag acggcagacc atatcattga
    15661 cttgggacct gagggcggtg tcggtggtgg aaccatcatc gtaacaggaa ctccagaaga
    15721 agtagctgcc aacgaagcca gctatacagg acactatttg aaaggaaagt tacatcatga
    15781 ataaacgcgt acaagcattt ctagctaaaa tgcaagaaaa agaactagat ggtatcatca
    15841 tcaacaatct taaaaacgtc tattatttga ctggtttttg gggctcaaac ggaacagtct
    15901 ttatcagtcg tgaccgtcag gtcttagtga cagactctcg ctatatcatc gcagctaagc
    15961 aagaaaccag tggttttgag attgtggctg atcgtgatga attggctgtc attgcaggaa
    16021 ttgttaagga catgggcttg actcgtatcg gttttgaaga tgagatttca gtgtcttatt
    16081 accaccgtat gcaggcagct tttgcaggtt tgaacttgct tccacaaact cagtttgtgg
    16141 aaggtcttcg tatgattaag gatgaggcag agattgcagc gattcgcaag gcttgttcta
    16201 tctcagacca agctttccgc gatgcgcttg actttatcaa accaggaaaa actgaaattg
    16261 agattgccaa cttccttgat ttccgcatgc gtgagttggg agcatctggc ttatcttttg
    16321 atacgatcct agctagcggt atcaattctt ctaaacccca tgcccatcca atgcacaaac
    16381 cagtggagtt gggagaagcc atcaccatgg acttcggctg tctctatgac cactatgtca
    16441 gtgatatgac ccggactatc tatctagggc atgttagcga tgagcaggca gagatttaca
    16501 atacggttct aaaagctaac caagccttga ttgaccaagc taaggcaggc ttaggtttcc
    16561 gtgactttga caaaatccct cgtgatatta tcattgaggc aggttatggt gactacttta
    16621 ctcacggcat tggccacggt attggtctgg atatccatga ggaaccctac tttagtcaga
    16681 cttctacaga aactattaag acaggtatgg ccttgaccga tgaaccaggt atctatatcg
    16741 aaggcaaata tggcgttcgt atcgaggatg atatcctgat tacagagaca ggttgtgaat
    16801 tattgaccct agctccaaaa gagttgatag tcatttagtt gtttgtcaag aaaaaagtac
    16861 gaaaatgacg aaaaaagtca aaaaaaatta aaaataggtc gcaagtcgga tgttttttat
    16921 ggtataatag actaaactga tagtaacatg tagcgaaagg ggtaggtaca tgattaaaat
    16981 ttatacagtc tcaagttgta ctagctgtaa aaaagcaaaa acctggctca atgcccacca
    17041 gttaagttat aaagaacaaa accttggtaa agaaggaatt acgagagaag aattactgga
    17101 tattctaacc aaaacagata acggaatagc cagcatcgtt tcgtctaaaa atcgctatgc
    17161 caaagccctt ggagtggata ttgaagattt gagtgtcaat gaagttctca atctgattat
    17221 ggaaacaccg agaattttaa agagcccaat ccttgtagat gaaaaacgcc tgcaagttgg
    17281 ctacaaggaa gacgatattc gtgccttcct accacgctct gtccgtaatg tagaaaatgc
    17341 agaagcacgt ttgcgtgcag ctctataaac atcaaggctg ggagcacttc ccagtcttat
    17401 tctattttaa tttcaaaaag aaagaagaaa gaaatgaaaa aaatagttct tgttagtcta
    17461 gctttccttt ttgtcctggt tggttgcgga cagaaaaaag aaactggacc agctacaaaa
    17521 acagaaaaag atacgcttca gtcggcattg ccagttattg aaaatgctga gaagaataca
    17581 gttgtaacta agactttggt cttgcccaag tcagatgatg gtagccagca gacacaaaca
    17641 attacttaca aagacaagac ttttttgagt ctagctatcc aacaaaaacg tccagtctct
    17701 gatgagttga agacttatat tgaccaacat ggagtggagg aaactcaaaa agctcttctt
    17761 gaagcggagg agaaggataa gtctatcatt gaagctcgta aattggcagg tttcaaactt
    17821 gaaacaaaac tattgagcgc aacggaactt caaacaacga ctagttttga ttttcaagtt
    17881 ctggatgtca agaaggcttc ccagttggaa catctgaaga atattggttt ggaaaatctt
    17941 ttgaaaaatg aaccaagcaa atatatttca gatagattgg caaatggcgc gacagaacaa
    18001 tagaaaatcc aaataaatag actgcctgaa ttgtagaagg taaggaatta tgctataatg
    18061 gtactattct aaggaaagag ggtgttagaa tgggatttac tgaagaaaca gtacgtttta
    18121 aattggacga ttccaataaa aaagaaatta gcgaaacttt gacagatgtt tatgcttcgt
    18181 tgaacgataa gggttacaac ccaattaacc aaatcgtagg ttacgtattg agtggagacc
    18241 ctgcctacgt tcctcgttat aataatgcac gaaatcaaat ccgtaagtat gagcgtgatg
    18301 aaatcgttga ggaattggtt cgctactacc tcaaaggaca aggagtcgat ctataaccta
    18361 tgagaattat gggattggac gtcggttcaa aaacggtagg ggtggcgatt agcgatccgc
    18421 ttggttttac agctcaaggg cttgaaatca tccagataaa tgaagaacaa ggccaatttg
    18481 gttttgaccg cgttaaggaa ttggttgata cttacaaggt ggaacgattt gtagtgggct
    18541 tgcctaaaaa catgaacaat acaagtggac cgcgcgtaga agctagtcaa gcctacggag
    18601 caaagctaga agagtttttt ggtttaccag tagactatca ggatgaacgc ttgacaacag
    18661 tggctgctga gcgcatgttg attgaacaag cagatatcag tcgcaataag cgcaagaaag
    18721 tcattgataa gttagcagct cagctgattt tacaaaatta tttagataga aaattttaat
    18781 ataaaggaga ggctatgtca cacgatcata accacgacca cgaagaacgt gaactaatca
    18841 cactagtaga tgagcaagga aatgaaacct tgtttgaaat ccttttgaca attgatggaa
    18901 aagaagaatt tggtaaaaac tatgttcttc tagtaccagt taacgcagaa gaagacgaag
    18961 acggacaagt tgaaatccaa gcttactcat tcatcgaaaa cgaagatgga acagaaggcg
    19021 aattgcaacc aatcccagaa gactcagaag acgaatggaa catgattgaa gaagtcttca
    19081 acagctttat ggaggagtga tacagtctgg gagactgtat cagcccaact cccagaaatg
    19141 gggaagagtt ggaacgtcca gtgaacattt ttaccccaac tcccagaaat ggggaagagt
    19201 tggaacgtcc agtggatgtt tttagcccat attcacattc atagtagcta gttattagct
    19261 aaccaggtaa gaggtcggga cgaaaatcct ggccttgttt ttattagtaa ccatacttta
    19321 atgcgataat tgattggact tttgtcaagg agatgtgtat gtttgaagta gaagaatggc
    19381 tccatagtcg gattggtttg aattttcgat caggtttggg tcgaatgcag caagcggtgg
    19441 atttgttagg aaatcctgag cagtcttacc ctattatcca cgtaacaggg actaatggga
    19501 aaggatctac cattgctttt atgagggaat tatttatggg gcatggcaaa aaagttgcga
    19561 cctttacctc ccctcatatc gtctctatca atgaccgaat ctgcattaat gggcagccta
    19621 tagcagacgc agactttatc cgtttgactg atcaggtcaa ggagatggag aaaacgcttc
    19681 tgcaaactcc tgcccagttg tccttttttg aattgctgac cttggttgct tttctttatt
    19741 ttagggagca ggaggtggat ttggttttat tagaagtggg aattggtggc ttacttgaca
    19801 cgaccaatgt ggtaactgga gagtttgctg tcatcacctc cattgggctt gaccatcaag
    19861 aaaccttggg tgatagtcta gaagcaattg cagagcagaa agctggtatt ttcaaggctg
    19921 gtaagaaggc agtgattgcg aaattgcctc cagaagctag gcttgcctgt cagaaaaaag
    19981 ccgaatcttt agctgttaac ctttatcagg caggtcaaga ttttttaatg ctgaatggtg
    20041 atttttcaag ctctttacta aatctttcac agctgaacat aggcttagaa ggagtctatc
    20101 agcaggagaa tgcagccttg gcgttgcaaa cttttcttct ttttatgaga gaaagaaagg
    20161 aagctgttga tgaacaggct gtaagaaagg ccttggaaca gacccattgg gctggtcgct
    20221 tggagcgtat tcgcccacag atttatttgg atggtgctca taacctccct gccttgactc
    20281 gcttggctga gtttatcaaa gaaaaagagc aggaaggcta tcgacctcaa atcctctttg
    20341 gatccttgaa acgtaaggat tatcaaggga tgttgggtta tctgactgaa aaattgcctc
    20401 aggtggaact caaggtgacc ggctttgact atcagggggc tttggacgaa agggatgtga
    20461 caggttacga tatagtttct tcttaccgag aatttatcag cgattttgaa gaaagggcag
    20521 acgctcaaga cttgctgttc gttacagggt ctctctattt tatctcagaa gtacggggct
    20581 acctgctgga ccgtgagcag ataaattgac tccatttttt gaacttgtta tactagggga
    20641 attgctggtt agagaaaatt tctctaaatt ggtagcagaa aggaaattca tcatgaaatt
    20701 aaaaagattc acactttctc ttgcttctct agcaagtttt agtctcttag tagcttgttc
    20761 acaaagagct caacaggttc aacagcctgt tgctcagcag caggtccaac aacctgctca
    20821 acagaatacc aatactgcaa atgcaggagg taaccaaaat caagcggctc cagtacaaaa
    20881 ccaacctgtt gctcaaccga ccgatattga tgggacttat actggtcagg atgacggaga
    20941 ccgtatcact ttagtggtaa ctggaacgac tggtacatgg actgagctcg aatctgacgg
    21001 ggatcagaaa gtcaaacagg ttacattgga ttcagcaaat caacgcatga ttattggcga
    21061 tgatgtcaaa atttacactg taaacggtaa tcaaatcgtc gtagatgata tggatagaga
    21121 cccatcggac caaatcgttt taactaaata agactatgca agtagaaagg gctggatttt
    21181 tcagcctttt tacttttacc tagaaaataa tcataaatac ttgccttcta ccgaatacct
    21241 gcgttatact agtatcaatt acttgttttt agcattcaaa ctatcaagga ggggatatga
    21301 aatataggaa atttcaatta ttgatgtcca agtatggctt tagtctttcg attatgctac
    21361 ttgaactttg tcttgttttt ggtctctttc tttatttagg acgcatggct cccattttat
    21421 ggattactgt cctcattcta ctgagtatca tcacaatcat ttcgatagtc aaccgtaata
    21481 cgactcctga gaataaggta acctggttgt tagtagcctt tgtgccagta tttggtccct
    21541 tgctctatct gatgtttggt gaaaggcgat tgtccaaaaa agaaatcaaa caactgaaga
    21601 agctaggctc tatgcatttc caagaagcaa atagccagct actaaaagag aaattaaaag
    21661 aaagtgacaa ggcagcttat ggagtcatca agtccttatt gagtatggat accaatgctg
    21721 acatctatga tcaaactgcc tctacatttt ttcctaacgg agaagctatg tggaaaaaga
    21781 tggtagaaga tcttaaaaag gctgagaaat ttattttctt ggaatattac attatagaag
    21841 aaggtttgat gtggaatcgc atactagata tactagagca aaaggtagct cagggtgtag
    21901 aggttaagat gctctatgat gatatcggct gtatggctac tttaacagga gattatgcac
    21961 atcgacttcg tcagctgggc atcgaggccc ataaattcaa taaagttatt cctcgtttga
    22021 cagtggctta taataacaga gatcatagaa aaatattgat tgttgatggt cagatagcct
    22081 atactggtgg ggtcaatctg gcagatgagt acattaacca cgtcgagaga tttggttatt
    22141 ggaaggatag tggaattcgc ttagacggac tagcagtaaa agctctgaca cgcttatttt
    22201 tgaccacttg gtacattaat cgaggagaaa ttagtgattt tgatcaatat catttagaaa
    22261 atcattctat cccgagtgac ggtttaacca ttccatacgg aagtggaccc aagccaattt
    22321 ttcgagcgca ggtagggaaa aaagtttatc agagtttaat caatcaagca acagaatcgg
    22381 tctatattac gacaccttat ttgattatag attatgattt aacagagaca atcaaaaatg
    22441 cagctatgag aggggtcgat gttcgaatta tcacccctta cataccagat aagaagttca
    22501 ttcagttagt cacgagagga gcttatcccg accttctttc tgctggtgtt cggatttatg
    22561 agtatagtcc aggttttatt catagtaagc agatgttggt agacgaagat tttgcggtgg
    22621 tggggacaat caatctcgac taccggagct tggtacacca ttatgaaaat gcagtcttac
    22681 tctataaaac tccttctata agggaaatcg cccgagattt tcgaaatata tttgcagatt
    22741 ctcaggaagt ctatcctcat tctatcaaaa cgagctggta tcaaaagctt gtaaaagaaa
    22801 tcgcccagct attcgcccct atcttataag aaatctagga cttaggatac aaaccagacc
    22861 tatttttctt gtcttttacg aatagaaaga aagaggaggg gaattcatgc taactaagga
    22921 agaagtgaat gatatgattg agttcaaaca gacacattta caccgctttc gggaaattga
    22981 aacctttgtg aaactatgca aaaaatcact caaacagcca tcgcaagctg acaatcctag
    23041 gactttttga tatactaaga cagataaatt gaacaggaga aacgatatga gtgtgtatgg
    23101 tagagtagaa gaagttcata aggagaatcg tgaacctcta gaataccaaa tcgaacaaga
    23161 atcgcatcat cgtgaatcaa gtcgtcttcc tttggtgaaa attttactat ggagtacgct
    23221 tgtaacgggg ataactctag gagtaccgct attactcgac ttaatgagtg cacaagaagt
    23281 gcaggatttt tatgcaggtt gggcgcttca tcagacaggg aagatttaca gcgactatta
    23341 tggaagtcaa ggtttgcttt attatttgct gacttacgtg agtcagggcg gatttttctt
    23401 tgccatcttt gagtggttag ccttggtagc aggaggattt ttccttttta gatcagcgga
    23461 caccttgaca gagcaaggag accaagctgg acatctggtg actatttttt acatgctagt
    23521 tacaggtctt gcttttggtg gaggctatgc gactctttta gcgcttcctt tcttattcgc
    23581 agcctttagt ttagttgcgg cttacctaag caatccaagc catgataagg gatttgtacg
    23641 gattgggcta gctttggcag gcggattttt ctttgctccc ttatcatcgc tcctgtttat
    23701 tgctgtagtg agtttaggct tgttggtctt taaccttggg catagacgct ttgcgcatgg
    23761 gttttatcag tttcttgcag tggctttagg tttttcactt gtcttttatc caactgccta
    23821 ctatagtgct gcaacaggaa gttttgggga tgcgattagt ggtattcgtt atcctattga
    23881 cagtattcgc tttgatttta cttctaaaat tttagagaat atgttttttt atggcttgtt
    23941 gtcccttggt ttgggatttg tggtttgtat ctttttaggt ctctttcaat ccaaaccctt
    24001 taaactatac gtcatttcgg ttcctgcaag tcttgtggta attttaggtt tgattttact
    24061 tttcttttca caagagcctc tgcacgcttc ttatttgatg gtcgtcttcc ctgttttcct
    24121 acttttattg gtaaccaata ttaagagtca acagaggggg cgtagtgcta gaagaagccg
    24181 aagagaaacg ccagttagcc tatggagtcg tttcttcaaa ggaaatctat atctgctagt
    24241 ttttgggttt gtctatcttt tgtctgttcc ttttttgatg aagtttgtcc tttatccagt
    24301 accttatcaa gaacgtaatc gtcttgctga tttggtaaaa gaggagacaa atacggaaga
    24361 tgctatctat gcatgggatg atactgcgac tctttatcgt aagagtgagc gcttgtcgcc
    24421 atcggcgatt ttgtccccgt tgcactatac agcaactgag gaaaatcgta ataagttact
    24481 taatgacttg aaagaaaaac aacctaaggt gattgtggta aatgataagg tggtagtctg
    24541 gtctgaagtg gaaacactct taaaagaaaa ttaccaacaa gtaaagactg attactcaga
    24601 gtttaaagtc tataaaatta aataaccaaa tcaatatctt gtgtattttt aaaaatttta
    24661 ggatttttaa cacaagatat tgatttttct ttttagagtg gtataatact ttttagaaag
    24721 aacattttag aaaagagcat gcatatgatt gcactagaag aaaaaattac aattttgcca
    24781 actctcttcg tcgagaaacg agatgggaga cgtgttgtat ttgatgtgga caagattgac
    24841 aaggctctcc acaaggcggc tgacaaggtt atggatgtga cacccctggt tgaaaaatgc
    24901 ctcaatgatc tgactgagcg aattattaca gaaattcata gtcgctttcc acagggaatt
    24961 aagatttacg aaattcaaaa tatcgtagaa catgaactcc ttgaagccaa agaatatgcg
    25021 ctggctgagg agtatattac ttatcggaca cagagggatt ttgagcgctc aaaagcgacg
    25081 gatatcaact ttagtattca taaacttctc aacaaagacc agacagttgt caatgaaaac
    25141 gctaataaag acagtgatgt ctttaacact cagcgtgatt tgacagcagg gattgttggg
    25201 aaatcaatcg gactgcaaat gcttcctaag cacgtagcca atgcccacca aaagggggat
    25261 atccactatc acgatttgga ctacagtccc tataccccta tgaccaactg ctgtttgatt
    25321 gattttaagg gtatgttgga aaatggtttt aagattggaa atgcagaggt agagagtccc
    25381 aagtctatcc agactgcgac agcacagatt tctcaaatca ttgccaacgt tgcttctagc
    25441 cagtacggtg gctgttcagc tgaccgtatc gatgaaattt tggcgcctta tgcagagaag
    25501 aattatcaaa aacatctcaa agatgcagaa gagtgggtat tgcctgaaaa acaggaagat
    25561 tacgcttgga agaaagcgca aaaggacatc tacgatgcca tgcaatctct tgagtatgaa
    25621 atcaatactc tcttcacttc aaatggacaa acacctttta cttcgttagg ttttggtctg
    25681 ggaaccagtc gttttgaacg agaaattcaa aaagctattt taaacattcg catcaagggt
    25741 cttggttcag aacaccgtac ggctatcttt cctaaactta tctttacgct taaaagaggc
    25801 ctcaacttag aggaaggaac tcccaactat gacatcaagc agttggctct agagtgtgca
    25861 accaagcgga tgtatccaga cgtcttgtct tatgataaga ttgttgattt gacaggttct
    25921 ttcaaggtgc ctatgggctg ccgttctttc cttcaagggt ggaaggatga aaatggtgta
    25981 gaagtcaatt caggtcgcat gaatctgggt gttgtgacgg ttaatctgcc tcgtattgct
    26041 cttgagtctg aaggtgatat gaataagttc tgggaaatct tcaacgagcg aatgaatatc
    26101 gcagaagatg ctcttgttta ccgtgtcgaa cgcactaaag aggcgacacc agcgaatgct
    26161 cctattcttt atcagtacgg tgcttttggc catcgtctag gtaaagaaga aagtgttgac
    26221 cagctcttta agaatcgtcg tgcgaccgtt tcgctgggct atatcggctt gtatgaagta
    26281 gcgacagttt tctttggtaa cagctgggaa agtaatccag atgctaagga attcacgcta
    26341 gacatcattc acgatatgaa acgccgtgta gaagagtggt cagaccaata tggctaccat
    26401 ttctctatct actcaacacc atccgaaagt ctgacagacc gtttctgccg actagatata
    26461 gacaagtttg gctctattcc tgatatcaca gacaaggaat actacaccaa ctctttccac
    26521 tacgatgttc gtaaaaatcc aacaccgttt gaaaaattgg actttgagaa agtctatccg
    26581 gaagcaggtg cgtcaggtgg tttcatccat tattgtgagt atccagtcct tcagcaaaat
    26641 ccaaaggcct tggaagctgt ctgggattat gcttatgacc gtgtaggcta tctaggcacc
    26701 aatactccga ttgaccgttg ctacaagtgt gactttgaag gggattttga accaactgag
    26761 agagggtttg cttgtccaaa ctgtggcaat agcgacccta aaacagtaga tgtggtgaaa
    26821 cgaacttgtg gctacctagg taatcctcaa gcaagaccga tggtcaacgg gcgtcacaag
    26881 gaaatcgctg cgcgtgtcaa acatatgaat ggttcaacga ttaaaatagc tgggcatcaa
    26941 gtaacaaatt agaaagaaat gaaatgggaa aatatcaact agacgataag gggcgcgcac
    27001 aagtgacccg ttatcacgag aaacactcta aaggtggagc tggtaagaaa gaacgcttgc
    27061 ttagcttcag agaacaattt ttaaacaaga acaagaaaaa ataaaagtga gagccagctc
    27121 tcgcttttct catagtggga ggtaaggatg gaattacgca gaccaagatt agcggataag
    27181 aaagctgttt tagatatgat gacagagttt gaaaaatttc agtcgcctca cgacggcggt
    27241 ttctgggata cagagaactt tgtgtatgaa gactggttag aaagcaatca ggaacaggaa
    27301 atggggatta atctgcctga aggatgggtt tctgcaattc agttagtggc tttttctgag
    27361 aaaggtcaag cagttggatt tcttaatctc cggttgcgcc tcagtaactt tctactagaa
    27421 gaaggtggcc acattggcta ctccattcgt ccatctgaaa gaggcaaggg ttatgcaaaa
    27481 gagactctcc gtcagggctt gcaagttgct aaggaaaaga acatcaagaa agctctggtg
    27541 acctgtagtg tgaataatcc tgctagcaga gcagtcattc tagcaaatgg tggaatattt
    27601 gaggatgctc gcaatggagt cgagcgttat tggatagagg tagcgaatga ataatccaaa
    27661 accacaagaa tggaaaagcg aggaacttag tcaaggtcgt atcattgact acaaggcctt
    27721 taactttgtg gacggcgaag gcgtgcgcaa ctctctctat gtatcaggct gtatgtttca
    27781 ctgcgaggga tgttataatg ttgcgacttg gtcttttaat gctggcattc cctatacagc
    27841 agaattagaa gagcagatta tggcagacct tgcccaaccc tatgttcaag gcttgacttt
    27901 gctgggaggg gagccttttc tcaatactgg gattctcttg ccacttgtta agcggattcg
    27961 gaaggaattg ccagacaagg acatctggtc ctggaccggc tacacttggg aagaaatgat
    28021 gttggaaact ccagataaac tggaattctt gtcactgatt gacattcttg tcgatggaag
    28081 atatgatcga actaagagaa atcttatgct ccagtttcga ggttcatcta accaacgaat
    28141 tatcgatgtg caaaaatcgc tcaaaagtgg gcaagtagtg atttgggaca agctcaatga
    28201 cggaaaagaa agctatgaac aggtgaagag agaatgaaga aaaaggactt agtagaccaa
    28261 ctagtctcag agatcgagac ggggaaagtc aggacactgg gaatatacgg tcatggagct
    28321 tcaggtaaat caacctttgc acaggaattg taccaagctt tagattctac tacagtaaat
    28381 ttgctagaga cagatcctta tatcacctca ggacgccatc tggtagtacc caaggacgcg
    28441 ccgaatcaaa aggtgacagc cagtctgcca gtggcgcatg aactggagag tttgcagaga
    28501 gatatccttg ccttgcaggc gggtatggat gtcttgacaa ttgaagaacc ttggaaggct
    28561 agtgaggtct tgtctggagc caaaccaatt ttgattgtcg aagggatgtc tgttggcttt
    28621 ctacccaagg aactctttga aaaaaccatc tgtttctaca cggatgagga gaccgaatta
    28681 aagcgacgcc ttgctagaga tacgactgtg agaaatcgcg atgcatcctt tatattggct
    28741 agccatcaga tgagacggga gcagtatctg cgatactata aagaaactga gtctaaggcg
    28801 gatatcttag tggaccaatc agaagataaa tttgatgtca agaggactta acttatgtat
    28861 agaagaaata tagaagaaaa tccctaattt tagaaagaaa aagtaggaaa acagtttcat
    28921 cccaaaaaaa cgaaaaaaca gcaacaaatc ccttgcaatc gcaggggctt tgtgttattc
    28981 tattatggtg ctgtaaatta cagccttagt tttgatgcaa gaggttgcga cacgctcggt
    29041 tgcattgcca cgcaacaccg cgtcggtttt cttgtggagc tagcctatta tcttaaatag
    29101 acgaaaagga gaaaaagatg gcaaacaaaa aaatccgtat ccgtttgaaa gcttacgaac
    29161 accgtacgct tgacacagcg gctgcaaaaa tcgtagaatc agctactcgt acaggtgcac
    29221 aagttgcggg tccaatccca cttccaactg aacgtagcct ctacacaatc attcgtgcga
    29281 ctcacaaata caaagactct cgcgaacaat ttgaaatgcg tacacacaaa cgtttgatcg
    29341 atatcgttaa cccaactcaa aaaacagttg atgccttgat gaaattggat cttccaagtg
    29401 gtgtaaacgt agaaatcaaa ctttaatcta aaatataaaa gagcagaggc tggtgtttca
    29461 atctaattga acacgggcta aactcggtgt gaaaaagata aacttcctag tgtctgctag
    29521 acactgcgtc agtttcctat tttcacgttg agtttgacgc cctttgtatc ttagacttga
    29581 gcataaaaaa cgctcgttaa aaactttttg aataaaaaat atagaaaagg aactattttc
    29641 tcatgacaaa aggaatctta gggaaaaaag tgggaatgac tcaaatcttc actgaagctg
    29701 gcgaattgat ccctgtaaca gttattgaag caactccaaa cgttgttctt caagttaaaa
    29761 ctgttgaaac agacggatac aacgctatcc aagttggttt cgatgacaaa cgcgaagtat
    29821 tgagcaacaa acctgctaaa ggacatgtag cgaaagctaa cacggctcct aagcgcttca
    29881 ttcgtgaatt caaaaacgtt gaaggcttgg aagttggtgc tgaaattaca gttgaaacat
    29941 tcgcagctgg agacgttgtt gacgtaacgg gtacttctaa aggtaaaggt ttccaaggtg
    30001 ttatcaaacg ccacggacaa tcacgtggac caatggctca cggttctcgt taccaccgtc
    30061 gtccaggttc tatggggcct gttgcaccta accgcgtatt caaaggtaaa aaccttgcag
    30121 gacgtatggg tggcgaccgc gtaacaattc aaaaccttga agttgtacaa gttgttccag
    30181 aaaagaacgt tatccttatc aaaggtaacg taccaggtgc taagaaatct cttatcacta
    30241 tcaaatcagc agttaaagct ggtaaataat aaagaaaggg gaaatcagtc acaatggcaa
    30301 acgtaacatt atttgaccaa actggtaaag aagctggcca agttgttctt agcgatgcag
    30361 tatttggtat cgaaccaaat gaatcagttg tgtttgatgt aatcatcagc caacgcgcaa
    30421 gccttcgtca aggaacacac gctgttaaaa accgctctgc agtatcaggt ggtggacgca
    30481 aaccatggcg tcaaaaagga actggacgtg ctcgtcaagg ttctatccgc tcaccacaat
    30541 ggcgtggtgg tggtgttgtc ttcggaccaa ctccacgttc atacggctac aaacttccac
    30601 aaaaagttcg tcgcctagct cttaaatcag tttactctga aaaagttgct gaaaacaaat
    30661 tcgtagctgt agacgctctt tcatttacag ctccaaaaac tgctgaattt gcaaaagttc
    30721 ttgcagcatt gagcatcgat tctaaagttc ttgttatcct tgaagaagga aatgaattcg
    30781 cagctctttc agctcgtaac cttccaaacg tgaaagttgc aactgctaca actgcaagtg
    30841 ttcttgacat cgcaaatagc gacaaacttc ttgtcacaca agcagctatc tctaaaatcg
    30901 aggaggttct tgcataatga atttgtatga tgttatcaaa aaacctgtca tcactgaaag
    30961 ctcaatggct caacttgaag caggaaaata tgtatttgaa gttgacactc gtgcacacaa
    31021 acttttgatc aagcaagctg ttgaagctgc tttcgaaggt gttaaagttg ccaatgttaa
    31081 cacaatcaac gtaaaaccaa aagctaaacg tgttggacgt tacactggtt ttactaacaa
    31141 aactaaaaaa gctatcatca cacttacagc tgattctaaa gcaatcgagt tgtttgctgc
    31201 tgaagctgaa taatctaagg aggaaatatc gtgggaattc gtgtttataa accaacaaca
    31261 aacggtcgcc gtaatatgac ttctttggat ttcgctgaaa tcacaacaag cactcctgaa
    31321 aaatcattgc ttgttgcatt gaagagcaag gctggtcgta acaacaacgg tcgtatcaca
    31381 gttcgtcacc aaggtggtgg acacaaacgt ttctaccgtt tggttgactt caaacgtaat
    31441 aaagacaacg ttgaagcagt tgttaaaaca atcgagtacg atccaaaccg ttctgcaaac
    31501 atcgctcttg tacactacac tgacggtgtg aaagcataca tcatcgctcc aaaaggtctt
    31561 gaagtaggtc aacgtatcgt ttcaggtcca gaagcagata tcaaagtcgg aaacgctctt
    31621 ccacttgcta acatcccagt tggtactttg attcacaaca tcgagttgaa accaggtcgt
    31681 ggtggtgaat tggtacgtgc tgctggtgca tctgctcaag tattgggttc tgaaggtaaa
    31741 tatgttcttg ttcgtcttca atcaggtgaa gttcgtatga ttcttggaac ttgccgtgct
    31801 acagttggtg ttgtcggaaa cgaacaacat ggacttgtaa accttggtaa agcaggacgt
    31861 agccgttgga aaggtatccg cccaacagtt cgtggttctg taatgaaccc taacgatcac
    31921 ccacacggtg gtggtgaagg taaagcacca gttggtcgta aagcaccatc tactccatgg
    31981 ggcaaacctg ctcttggtct taaaactcgt aacaagaaag cgaaatctga caaacttatc
    32041 gttcgtcgtc gcaacgagaa ataatattaa actagtcgct taagcaacta gtaaatccgc
    32101 cagctcggta gcgctccata ggagtgcaag ccgctgtggt acaacattta aaggagaaaa
    32161 tataaaaatg ggacgcagtc ttaaaaaagg acctttcgtc gatgagcatt tgatgaaaaa
    32221 agttgaagct caagctaacg acgaaaagaa aaaagttatt aaaacttggt cacgtcgttc
    32281 aacgatcttc ccaagtttca ttggttacac tattgcagtt tatgacggac gtaaacacgt
    32341 acctgtttac atccaagaag acatggtagg ccacaaactt ggtgaatttg caccaactcg
    32401 tacttacaaa ggtcacgctg cagacgacaa gaaaacacgt agaaaataag gagaacataa
    32461 atggcagaaa ttacttcagc taaagcaatg gctcgtacag tacgtgtttc acctcgtaaa
    32521 tcacgtcttg ttcttgataa catccgtggt aaaagcgtag ccgatgcaat cgcaatcttg
    32581 acattcactc caaacaaagc tgctgaaatc atcttgaaag ttttgaactc agctgtagct
    32641 aacgctgaaa acaactttgg tttggataaa gctaacttgg tagtatctga agcattcgca
    32701 aacgaaggac caactatgaa acgtttccgt ccacgtgcga aaggttcagc ttcaccaatc
    32761 aacaaacgta cagctcacat cactgtagct gttgcagaaa aataaggagg taaaatcgtg
    32821 ggtcaaaaag tacatccaat tggtatgcgt gtcggcatca tccgtgattg ggatgccaaa
    32881 tggtatgctg aaaaagaata cgcggattac cttcatgaag atcttgcaat ccgtaaattc
    32941 gttcaaaaag aacttgctga cgcagcagtt tcaactattg aaatcgaacg cgcagtaaac
    33001 aaagttaacg tttcacttca cactgctaaa ccaggtatgg ttatcggtaa aggtggtgct
    33061 aacgttgatg cactccgtgc aaaacttaac aaattgactg gaaaacaagt acacatcaac
    33121 atcatcgaaa tcaaacaacc tgatttggat gctcaccttg taggtgaagg aattgctcgt
    33181 caattggagc aacgtgttgc tttccgtcgt gcacaaaaac aagcaatcca acgtgcaatg
    33241 cgtgctggag ctaaaggaat caaaactcaa gtatcaggtc gtttgaacgg tgcagatatc
    33301 gcccgtgctg aaggatactc tgaaggaact gttccgcttc acacacttcg tgcagatatc
    33361 gattacgctt gggaagaagc agatactaca tacggtaaac ttggtgttaa agtatggatc
    33421 taccgtggtg aagttcttcc agctcgtaaa aacactaaag gaggtaaata accaatgtta
    33481 gtacctaaac gtgttaaaca ccgtcgtgag ttccgtggaa aaatgcgcgg tgaagcaaaa
    33541 ggtggaaaag aagtagcatt cggtgaatac ggtcttcaag ctacaactag ccactggatc
    33601 actaaccgcc aaatcgaagc tgctcgtatc gccatgactc gttacatgaa acgtggtggt
    33661 aaagtttgga ttaaaatctt cccacacaaa tcatacactg ctaaagctat cggtgtgcgt
    33721 atgggatctg gtaaaggggc acctgaaggt tgggtagcac cagttaaacg tggtaaagtg
    33781 atgttcgaaa tcgctggtgt atctgaagag attgcacgtg aagcgcttcg acttgctagc
    33841 cacaaattgc cagttaaatg taaattcgta aaacgtgaag cagaataagg agaaggcatg
    33901 aaacttaatg aagtaaaaga atttgttaaa gaacttcgtg gtctttctca agaagaactc
    33961 gcgaagcgcg aaaacgaatt gaaaaaagaa ttgtttgaac ttcgtttcca agctgctact
    34021 ggtcaattgg aacaaacagc tcgcttgaaa gaagttaaaa aacaaatcgc tcgcatcaaa
    34081 acagttcaat ctgaagcgaa ataatagact agggaaggag aaatttcaat ggaacgcaat
    34141 aatcgtaaag ttcttgttgg acgtgttgta tctgacaaaa tggacaagac aatcacagtt
    34201 gtagttgaaa caaaacgtaa ccacccagtc tatggtaaac gtattaacta ctctaaaaaa
    34261 tacaaagctc atgatgaaaa caatgttgcc aaagaaggcg atatcgtacg tatcatggaa
    34321 actcgcccgc tttcagctac aaaacgtttc cgtcttgtag aagttgttga agaagcggtc
    34381 atcatctaat caaacctgaa aggagaaaac tgaaatgatt caaacagaaa ctcgtttgaa
    34441 agtcgcagac aacagcggtg ctcgcgaaat cttgactatc aaagttcttg gtggttcagg
    34501 acgtaaattt gcaaacatcg gtgatgttat cgtggcatct gtaaaacaag ctactcctgg
    34561 tggtgcggtt aaaaaaggtg acgttgttaa agcagttatc gttcgtacta aatcaggtgc
    34621 tcgtcgtgct gatggttcat acatcaaatt tgacgaaaac gcagcagtta tcatccgtga
    34681 agacaaaact cctcgcggaa cacgtatctt tggcccagtt gcacgtgaat tgcgtgaagg
    34741 tggcttcatg aagatcgtgt cacttgctcc agaagtactt taatttttag gaacaaacta
    34801 gtcccctagc ttcaagctag ggtgccctta tgggcgtaag aaaaatcaag gagaaaccta
    34861 atgtttgtaa aaaaaggcga caaagttcgc gtaatcgctg gtaaagataa gggaacagaa
    34921 gctgttgtcc ttactgccct tccaaaagta aacaaagtta tcgttgaagg tgttaacatt
    34981 gttaagaaac accaacgtcc aactaacgag cttcctcaag gtggtatcat cgagaaagaa
    35041 gcagctatcc acgtatcaaa cgttcaagtt ttggacaaaa atggtgtagc tggtcgtgtt
    35101 ggatacaaat ttgtagacgg taaaaaagtt cgctacaaca aaaaatcagg cgaagtgctt
    35161 gattaatcac gaaggaaagg agaagtataa tggcaaatcg tttaaaagaa aaatatctta
    35221 atgaagtagt tcctgctttg acagaacaat tcaactactc atcagtgatg gctgtgccta
    35281 aagtagataa gattgttttg aacatgggtg ttggtgaagc tgtatcaaac gctaaaagcc
    35341 ttgaaaaagc tgctgaagaa ttggcactta tctcaggtca aaaaccactt atcactaaag
    35401 ctaaaaaatc aatcgccggc ttccgtcttc gtgaaggtgt tgcgatcggt gcaaaagtta
    35461 cccttcgtgg tgaacgtatg tacgaattct tggataaatt ggtatcagtt tcacttccac
    35521 gtgtacgtga cttccacggt gtcccaacaa aatcatttga tggacgcggg aactacacac
    35581 ttggtgtgaa agaacaatta atcttcccag aaatcaactt cgatgacgtt gacaaaactc
    35641 gtggtcttga catcgttatc gtaacaactg ctaacactga cgaagagtca cgtgcattgc
    35701 ttacaggcct tggaatgcct tttgcaaaat aatataggag gtaaatctaa tggctaaaaa
    35761 atcaatggta gctagagagg ctaaacgcca aaaaattgtt gaccgttatg ctgaaaaacg
    35821 tgctgcatta aaggcggcag gggactacga aggtttatct aaattacctc gcaacgcctc
    35881 accgactcgt ttacataatc gttgtagggt tacggggcgc ccacattcag tttaccgcaa
    35941 atttggtctg agtcgtatcg cttttcgcga acttgcgcat aaaggtcaaa ttcctggtgt
    36001 aacaaaagca tcttggtaat ttaagatatc aagagcgtca aaactccaag taaaaatagg
    36061 aaacttgacg aagaaactaa agtttctagg aaagtttatc tttttcacac agagtttagc
    36121 ccgggttcaa ttgggcttgc caatttgaac acgagctaca gctttggcaa aaaagaccaa
    36181 tttgctttgg agcattgctt ctgcattaaa ttgtctattt ttgctcgtgc tgttacgctc
    36241 tttgtatcat gtattaacta gcaagtgcaa cttgcaaact actagtaaga ggagaaaaac
    36301 aaaatggtta tgactgaccc aatcgcagac ttcctaactc gtattcgtaa tgctaaccaa
    36361 gctaaacacg aagtacttga agtacctgca tcaaacatca aaaaagggat tgctgaaatc
    36421 cttaaacgcg aaggttttgt aaaaaacgtt gaaatcattg aagatgacaa acaaggcgtc
    36481 atccgtgtat ttcttaaata cggaccaaat ggtgagaaag ttatcactaa cttgaaacgt
    36541 gtttctaaac caggacttcg tgtctacaaa aaacgtgaag accttccaaa agttcttaac
    36601 ggacttggaa ttgccatcct ttcaacttct gaaggtttgc ttactgataa agaagcacgc
    36661 caaaagaatg ttggtggtga ggttatcgct tacgtttggt aaaatcaaga tacaaagctc
    36721 gtaaagaaca aagcaaaatt aggaagttgg agaagtttgt ttacaaacaa gccaacttat
    36781 ctattttgca cagttcttag agcgtgttca gttcagctct tgaactaaat aagtatctga
    36841 accccgtgaa aactggccgt tctggcctga caatttaaca ggagaaaata aacatgtcac
    36901 gtattggtaa taaagttatc gtgttgcctg ctggtgttga actcgctaac aatgacaacg
    36961 ttgtaactgt aaaaggatct aaaggagaac ttactcgtga gttctcaaaa gatattgaaa
    37021 tccgtgtgga aggtactgaa ataactcttc accgtccaaa cgattcaaaa gaaatgaaaa
    37081 ctatccacgg aactactcgt gcccttttga acaacatggt tgttggtgta tcagaaggat
    37141 tcaagaaaga acttgaaatg cgtggggttg gttaccgtgc acagcttcaa ggatctaaac
    37201 ttgttttggc tgttggtaaa tctcatccag acgaagttga agctccagaa ggaattactt
    37261 ttgaacttcc aaacccaaca acaatcgttg ttagcggaat ttcaaaagaa gtagttggtc
    37321 aaacagctgc ttacgtacgt agccttcgtt caccagaacc atataaaggt aaaggtatcc
    37381 gttacgttgg tgaattcgtt cgccgtaaag aaggtaaaac aggtaaataa tgttgagtgg
    37441 ttgatcatca accaccaacc tattttccaa ctttgtgcat agcacacgat ttaaaactaa
    37501 agaggtgaaa actgtgattt caaaaccaga taaaaacaaa ctccgccaaa aacgccaccg
    37561 tcgcgttcgc ggaaaactct ctggaactgc tgatcgccca cgtttgaacg tattccgttc
    37621 taatacaggc atctacgctc aagtgattga tgacgtagcg ggtgtaacgc tcgcaagtgc
    37681 ttcaactctt gataaagaag tttcaaaagg aactaaaact gaacaagccg ttgctgtcgg
    37741 taaactcgtt gcagaacgtg caaacgctaa aggtatttca gaagtggtgt tcgaccgcgg
    37801 tggatatcta tatcacggac gtgtgaaagc tttggctgat gcagctcgtg aaaacggatt
    37861 gaaattctaa taggaggaca ctagaaaatg gcatttaaag acaatgcagt tgaattagaa
    37921 gaacgcgtag ttgctgtcaa ccgtgttaca aaagttgtta aaggtggacg tcgtcttcgt
    37981 ttcgcagctc ttgttgttgt tggtgaccac aatggtcgcg taggatttgg tactggtaaa
    38041 gctcaagaag ttccagaagc aatccgtaaa gcagtagatg atgctaagaa aaacttgatc
    38101 gaagttccta tggttggaac aacaatccca cacgaagttc tttcagaatt cggtggagct
    38161 aaagtattgt tgaaacctgc tgtagaaggt tctggagttg ccgctggtgg tgcagttcgt
    38221 gccgttgtgg aattggcagg tgtggcagat attacatcta aatcacttgg ttctaacact
    38281 ccaatcaaca ttgttcgtgc aactgttgaa ggtttgaaac aattgaaacg cgctgaagaa
    38341 attgctgccc ttcgtggtat ttcagtttct gatttggcat aagaaagggg ataaaatggc
    38401 tcaaattaaa attactttga ctaagtctcc aatcggacgc attccatcac aacgtaaaac
    38461 tgttgtagca cttggacttg gcaaattgaa cagctctgtt attaaagaag ataacgctgc
    38521 tatccgtggt atgatcacag cagtatctca cttagtaaca gttgaagaag taaactaatg
    38581 aagttttagg ggatgtgcac tgtaccatcc cctaaaacta gatatagtca tctatgatga
    38641 catcgtatag gcgagttgat gggggagaca accttttctc ccttatcggc gctagcattt
    38701 tacaaaagag gagaaaataa aaatgaaact tcatgaattg aaacctgcag aaggttctcg
    38761 taaagtacgt aaccgcgttg gtcgtggtac ttcatcaggt aacggtaaaa catctggtcg
    38821 tggtcaaaaa ggtcaaaaag ctcgtagcgg tggcggagtt cgccttggtt ttgaaggtgg
    38881 acaaactcca ttgttccgtc gtcttccaaa acgtggattc actaacatca acgctaaaga
    38941 atacgcaatt gtgaaccttg accaattgaa cgtctttgaa gatggtgctg aagtaactcc
    39001 agttgttctt atcgaagcag gaattgttaa agctgaaaag tcaggtatta aaattcttgg
    39061 taacggtgag ttgactaaga aattgactgt gaaagcagct aaattctcta aatcagctga
    39121 agaagctatc actgctaaag gtggttcagt agaagtcatc taagagaggt gacctatgtt
    39181 ttttaaatta ttaagagaag ctcttaaagt caagcaggtt cgatcaaaaa ttttatttac
    39241 aatttttatc gttttggtct ttcgtatcgg aactagcatt acagttcctg gtgtgaatgc
    39301 caatagcttg aatgctttaa gtggattatc cttcttaaac atgttgagct tggtgtcggg
    39361 gaatgcccta aaaaactttt cgatttttgc cctaggagtt agtccctata tcaccgcttc
    39421 tattgttgtc caactcttgc aaatggatat tttacccaag tttgtagagt ggggtaaaca
    39481 aggggaagta ggtcgaagaa aattgaatca agctactcgt tatattgctc tagttctcgc
    39541 ttttgtgcaa tctatcggga ttacagctgg ttttaatacc ttggctggag ctcaattgat
    39601 taaaactgct ttaactccac aagtttttct gacgattggt atcatcttaa cagctggtag
    39661 tatgattgtc acttggttgg gtgagcaaat tacagataag ggatacggaa acggtgtttc
    39721 catgattatc tttgccggga ttgtttcctc aattccagag atgattcagg gcatctatgt
    39781 ggactacttt gtgaacgtcc caagtagccg tatcacttca tctatcattt tcgtaatcat
    39841 tttgattatt actgtattgt tgattattta ctttacaact tatgttcaac aagcagaata
    39901 caaaattcca atccaatata ctaaggttgc acaaggtgct ccatctagct cttaccttcc
    39961 gttaaaagta aaccctgctg gagttatccc tgttatcttt gccagttcga ttactgcagc
    40021 gcctgcggct attcttcagt ttttgagtgc cacaggtcat gattgggctt gggtaagggt
    40081 agcacaagag atgttggcaa ctacttctcc aactggtatt gccatgtatg ctttgttgat
    40141 tattctcttt acattcttct atacgtttgt acagattaat cctgaaaaag cagcagagag
    40201 cctacaaaag agtggtgcct atatccatgg agttcgtcct ggtaaaggta cagaagaata
    40261 tatgtctaaa cttcttcgtc gtcttgcaac tgttggttcc ctcttccttg gtgtgatttc
    40321 cattttaccg attgcagcta aagatgtatt tggtctttct gatgttgttg cctttggtgg
    40381 aacaagtctc ttgatcatta tctctacagg tatcgaagga atcaagcaat tggaaggtta
    40441 cctattgaaa cgtaagtatg ttggtttcat ggacagaaca gaataaaagt atttactgaa
    40501 tcagtaaata ctgagggagt ggaggtttaa actctgacat ttgtaagagt tggatctccc
    40561 ctcttctatt ttgtttttaa atcggggtga aaagactttt tgcttctatt taaaaataaa
    40621 ataaggagat caaatcatga atcttttgat tatgggctta cctggtgcag gtaagggaac
    40681 tcaagcagca aaaatcgtag aacaattcca tgttgcacat atctcaacag gtgatatgtt
    40741 ccgcgctgca atggcaaatc aaactgaaat gggtgttctt gctaagtcat atattgacaa
    40801 gggtgaattg gttcctgacg aagttacaaa tggaatcgta aaagaacgcc tttcacaaga
    40861 tgatattaaa gaaacaggat tcttattgga tggttaccca cgtacaattg aacaagctca
    40921 tgccttggac aaaacattgg ctgaacttgg cattgaacta gaaggtgtta tcaatattga
    40981 agtgaaccct gacagccttt tggaacgttt gagtgggcgt atcatccacc gcgtaactgg
    41041 agaaactttc cacaaggtct ttaacccacc agttgactat aaagaagaag attactacca
    41101 acgtgaagat gataagcctg agacagtaaa acgtcgtttg gatgttaata ttgctcaagg
    41161 agaaccaatc attgctcact accgtgccaa aggtttggtt catgacatcg aaggtaatca
    41221 agatatcaat gatgtcttct cagatattga aaaagtattg acaaatttga aataaagcgt
    41281 ttttcacact tgcaaaaatc cgctacaaat gttatactga gatagtctga cttataattg
    41341 ttgtctctgt gtctagaggc atcgaatcga aatttatgga ggtgcttttg cgtggcaaaa
    41401 gacgatgtga ttgaagttga aggcaaagta gttgatacaa tgccgaatgc aatgtttacg
    41461 gttgaacttg aaaatggaca tcagatttta gcaacagttt ctggtaaaat tcgtaaaaac
    41521 tatattcgta ttttagcggg agatcgtgtt actgtcgaaa tgagtccata tgacttgaca
    41581 cgtggacgta tcacttaccg ctttaaataa tcgaaaaact tggagggata agaaatgaaa
    41641 gtaagaccat cggtcaaacc aatttgcgaa tactgtaaag ttattcgtcg taatggtcgt
    41701 gttatggtaa tttgcccagc aaatccaaaa cacaaacaac gtcaaggata agatagaaag
    41761 gagaaaacat ggctcgtatt gctggagttg atattccaaa tgacaaacgc gtagtaatct
    41821 cattgactta tgtttatggt atcggacttg caacatctaa gaaaattttg gctgctgctg
    41881 gaatctcaga agatgttcgt gtacgtgatc ttacatcaga tcaagaagat gctatccgtc
    41941 gtgaagtgga tgcaatcaaa gttgaaggtg accttcgtcg tgaagtaaac ttgaacatca
    42001 aacgtttgat ggaaatcggt tcataccgtg gtatccgtca ccgtcgtgga cttcctgtcc
    42061 gtggacaaaa cactaaaaac aacgcccgca ctcgtaaagg taaagctgtt gcgattgctg
    42121 gtaagaaaaa ataatatagg aggtaaaagt cttggctaaa ccaacacgta aacgtcgtgt
    42181 gaaaaagaat atcgaatctg gtattgctca tattcacgct acatttaata acactattgt
    42241 tatgattact gatgtgcatg gtaatgcaat tgcttggtca tcagctggtg ctcttggttt
    42301 caaaggttct cgtaaatcta caccattcgc tgctcaaatg gcttctgaag ctgctgctaa
    42361 atctgcacaa gaacacggtc ttaaatcagt tgaagttact gtaaaaggtc caggttctgg
    42421 tcgtgagtca gctattcgtg cgcttgctgc cgctggtctt gaagtaacag caattcgtga
    42481 tgtgactcca gtgccacaca atggtgctcg tcctccaaaa cgtcgccgtg tataatcatc
    42541 gcattacact gcttttcgtt taagagggag taactaaatg atcgagtttg aaaaaccaaa
    42601 tataacaaaa attgatgaaa ataaagatta tggcaagttt gtaatcgaac cacttgaacg
    42661 tggctacggt acaactcttg gtaactctct tcgtcgtgta cttctagctt ctctaccagg
    42721 agcagctgtg acatctatca acattgatgg tgtgttacat gagtttgaca cagttccagg
    42781 tgttcgtgaa gacgtgatgc aaatcattct gaacattaaa ggaattgcag tgaaatcgta
    42841 cgttgaagac gaaaaaatca tcgaactgga tgttgaaggt cctgctgaag taacagctgg
    42901 tgacattttg acagatagcg atattgaaat tgtaaatcca gatcattatc tctttacaat
    42961 cggtgaaggt tcttctctaa aagcgactat gactgttaac agtggtcgtg gatatgtacc
    43021 tgctgatgaa aataaaaagg ataatgcacc agttggaaca cttgctgtag attctattta
    43081 tacaccagtt acaaaagtca actatcaagt ggaacctgct cgtgtaggta gcaatgatgg
    43141 tttcgacaaa ttaacccttg aaatcttgac aaatggaaca attattccag aagatgcttt
    43201 agggctttca gcacgtattt tgacagaaca tcttgatttg tttacaaatc ttactgagat
    43261 tgctaagtca actgaagtga tgaaagaagc tgatactgaa tctgacgacc gtattttaga
    43321 tcgtacgatt gaggaactgg acttgtctgt gcgttcatac aactgtttaa aacgtgccgg
    43381 tatcaatact gtgcatgatt tgacagaaaa atctgaagca gagatgatga aagtacgaaa
    43441 tcttggacgc aagagtttgg aagaagtgaa actcaaactc attgatttgg gtcttggatt
    43501 aaaagataaa taaaggagga atacatggct taccgtaaac taggacgcac tagctcacaa
    43561 cgtaaagcaa tgcttcgcga tttgacaact gaccttttga tcaacgaatc aatcgtgaca
    43621 actgaagctc gtgctaaaga aatccgtaaa actgttgaaa aaatgattac tctaggtaaa
    43681 cgtggtgatt tgcatgcacg tcgtcaagca gctgctttcg tacgtaatga aatcgcatct
    43741 gaaaactatg atgaagcaac tgataagtac acttctacta cagcacttca aaaattgttc
    43801 tcagaaatcg cacctcgtta tgctgaacgt aacggtggat acactcgtat ccttaaaact
    43861 gaatcacgtc gtggtgatgc agcgccaatg gcgatcatcg aattagtata aaatcatcaa
    43921 ttttgttgag tgttatgatg atggagtctt gtgctcttag tctagctctg gtctaccgct
    43981 aggatttcgg tcctagcggg aacactcatc ataagttggg atagtagacg cttgtttacg
    44041 aaattgtttt tttcttaaga acaacttcgt aagcaggcgt ttttgagtat tttcgttaga
    44101 attatgctat actatttgaa aagaatcctg tttaatgtta aggtttctta ttttaagaag
    44161 aattggagtt tacttatgaa agccattata actgttgttg gtaaagataa atctggaatt
    44221 gttgcaggtg tttctggtaa aattgcagaa ttaggattga atattgacga tatctctcaa
    44281 actgtcttgg atgaatattt tacgatgatg gctgttgtat ctagtgatga aaagcaagat
    44341 tttacctatc ttcgtaatga atttgaagct tttgggcaaa ctttgaatgt aaaaatcaat
    44401 attcagagtg cagcgatttt cgaagctatg tataatatct aggaggtcat catggatatt
    44461 agacaagtta ctgaaaccat cgccatgatt gaggagcaaa acttcgatat tagaaccatt
    44521 accatgggga tttctctttt ggactgtatc gatccagata tcaatcgtgc tgcggagaaa
    44581 atctatcaaa aaattacgac aaaggcggct aatttagtag ctgttggtga tgaaattgcg
    44641 gctgagttgg gaattcctat cgttaataag cgtgtatcgg tgacacctat ttctctgatt
    44701 ggggcagcga cagatgcgac ggactacgtg gttctggcaa aagcgcttga taaggctgcg
    44761 aaagagattg gtgtggactt tattggtggt ttttctgcct tagtacaaaa aggttatcaa
    44821 aagggagatg agattctcat caattccatt cctcgcgctt tggctgagac ggataaggtc
    44881 tgctcgtcag tcaatatcgg ctcaaccaag tctggtatta atatgacggc tgtggcagat
    44941 atgggacgaa ttatcaagga aacagcaaat ctttcagata tgggagtggc caagttggtt
    45001 gtattcgcta atgctgttga ggacaatcca tttatggcgg gtgcctttca tggtgttggg
    45061 gaagcagatg ttatcatcaa tgtcggagtt tctggtcctg gtgttgtgaa acgtgctttg
    45121 gaaaaagttc gtggacagag ctttgatgta gtagccgaaa cagttaagaa aactgccttt
    45181 aaaatcactc gtatcggtca attggttggt caaatggcca gtgagagact gggtgtggag
    45241 tttggtattg tggacttgag tttggcacca acccctgcgg ttggagactc tgtggcacgt
    45301 gtccttgagg aaatggggct agaaacagtt ggcacgcatg gaacgacggc tgccttggcc
    45361 ctcttgaacg accaagttaa aaagggtgga gtgatggcct gcaaccaagt cggtggttta
    45421 tctggtgcct ttatccctgt ttctgaggat gaaggaatga ttgctgcagt gcaaaatggc
    45481 tctcttaatt tagaaaaact agaagctatg acggctatct gttctgttgg attggatatg
    45541 attgccatcc cagaagatac gcctgctgaa actattgcgg ctatgattgc ggatgaagca
    45601 gcaatcggtg ttatcaacat gaaaacaaca gctgttcgta tcattcccaa aggaaaagaa
    45661 ggcgatatga ttgagtttgg tggtctatta ggaactgcac ccgttatgaa ggttaatggg
    45721 gcttcgtctg tcgacttcat ctctcgcggt ggacaaatcc cagcaccaat tcatagtttt
    45781 aaaaattaag aaaataggag aaattttaag ttctatttaa gattagacgt gtatactata
    45841 atcattaaat aaagacctcc taatattatt tgaaacagat aacactgaat tagtttgaat
    45901 ttgattttca tctaatatct ttatttaatg aactcctaaa ctttttcata ataatctcct
    45961 tcaaaagtcg cctgtatggg tggcttttat tttatcattc atgatataat agaagcaaac
    46021 ggaggacgga aaatggtaaa agtacgattg tatttggtac gtcatggcaa gaccatgttt
    46081 aacacgattg gtcgcgcgca aggttggagc gatactccct taactgctga aggtgaacga
    46141 gggattcaag agttaggaat cggtttgcga gaatctgatc tacagtttga gcgtgcttat
    46201 tcgagtgatt ctggtcgtac cattcagacc atgggaatta tccttgaaga acttggcttg
    46261 cagggggaaa tcccttatcg catggacaag cgtatcagag aatggtgttt cggtagtttt
    46321 gatggagcct atgatggcga tcttttcatg ggcattattc ctcgtatctt taatgtggac
    46381 cacgttcacc aattgtctta tgctgaactg gctgagggct tggtagaggt cgatacagct
    46441 ggttgggctg aaggctggga aaaactcagt ggccgaatca aggaaggctt tgaaatgatt
    46501 gcaaaagaaa tggaagatca aggtggaggt aacgcccttg ttgtcagcca tggaatgact
    46561 attggaacca ttgtttatct gattaatggc atgcatccgc atggtctgga taatggtagc
    46621 gtgacaatcc ttgaatatga ggacggccag tttagggttg aagttgtcgg tgaccgtagt
    46681 taccgagagc taggacgtga gaagatggaa gaaggctcta tttaatcagt ctagacttgc
    46741 ttgccatgag ctagggattt gataagaata tcaagataag aaaaaacagc cgagggcact
    46801 cctttcggct gtttttgatg tggaaaacta aagtgtaatg ctattgcttt tagagatttt
    46861 cataaacaag agcaaggaac ctactgttag aacagtcagg atagttgaca aggttgcggc
    46921 tacaccgtaa tttcctctga gaacctctgt ataaatagct acagtcattg ttcttgtttt
    46981 gacattgtag aggaggatag aagtagagag ttttgaaatc attgtgactc aagataagat
    47041 ggctccagaa atgataccag atagcatcat tggagttgta atcttagcaa aggtattgag
    47101 acgactactt cctaagcttt cagcagcttc ttcaatactt ggtgctattt gttgtaagct
    47161 agcaacagat gagcgaatag tataaggtaa tcttctggca gatagagaca taatcaagat
    47221 gaaagcagtc cctgtaatca taagaaatcc acttccaaat agaccagtat tgaaggaaga
    47281 aatgaaggca atccctagaa cggttcctgg tacaatataa ggtaccatac tgaggctgtc
    47341 aattaagttt gtaaacaaat tccgttttct aacggctagg taggagataa atgtcgcaaa
    47401 tagaacaact agaactaagg caatcaaagg gatacgaatg gtattgaaaa tagcagatcc
    47461 catacgatgg aaagctacct tgtaactgtt tggagaataa cctttaacag ataccatacc
    47521 tgatgttttt aggaaagagg tataaattaa gtagatttga ggtaaaacag agataaagat
    47581 aattccgtag actgttgcat aaatggcagc catttttcct tttgtagttt ttttaggctc
    47641 aattggatgg agcagattca tgctgaaact gtagcggttt gcaatgtgtt tttggataag
    47701 gaaaattgcc aaggcaatga taatcgccat aattgcaaaa gcagaatttc ctccaacctc
    47761 gctaataaat tgggtataaa tcaggacagg gaaagtccga tacccttcgc caatcaacat
    47821 aggcgttcca aagtctgaga atgctctcat aaatacaagc aaggagctgc tagtaaggtt
    47881 ggaactagga gaggtaaaac aaccgttacg ataggtttaa atccgaagga ccccatgctt
    47941 tcagctgctt caagtagaga attgtcaata ctgttcattg ttccagcaac atatagaaat
    48001 accagtggga atagttgcag tgtaaagaca agtacaattc ctttgaatca ataaatatcg
    48061 atagctggaa gataaagggc atttgtcaaa aatttagtga tgacctcatt tcgtcctagc
    48121 aagagaaccc aggagtaggc tcctacgaaa ggagctgaca tggaagcaat gataatcaat
    48181 atttgtagaa atttcttccc cttgaagtca tacatagaga agagataagc taatagggtt
    48241 cctacaacta aggaagtgat agtagcggta atggaaacct tgaaactgtt gactagtgtc
    48301 tcagagtagt aggctttact aaagaaagtg acaaaattag ctagtgagaa ttgtccttca
    48361 tgtataagtg cttgcttgag cacggtaacg ataggataaa cgagaaagat aggataggta
    48421 agaaagagga agaaagagga aactgtccaa atatttagtt ttttacgttc catggttgac
    48481 tccttttatc aggttttggg aaccatctgc agaaaagatg tttaattttt gcgtattgat
    48541 tcgtagacga atacgattgc ctttttgtag atcttcttca aaagttgatt cttcactaac
    48601 ttgaattttt gaggcaaaac ctgtctcaat gaaataatcc gtatttagtc caagatagac
    48661 gctatctcta atagttcctt caatatctcc agattcatct ttgataaact cttcgggacg
    48721 aatgcttaca tgaatagctt gctcctcaac ctgatcaaga gctggcattc gaagggcata
    48781 gccatctgaa aagacgatat aagcgccgtc gctccgtttt tcaagattgg cagggataat
    48841 atttgtgcgt ccgataaagg ttgccacaaa ctcattagct ggtttatgat agagttcttt
    48901 tggtcggccg atttgttgga tcaccccatc tttcataaca gcaatttggt ctgaaatagc
    48961 catggcttct tcttggtcgt gggttacata aacagttgta attcccactt cgtgttggat
    49021 ttctcggatg gcttgacgca tatccaagcg aagtttggcc tccagattac taagtggctc
    49081 gtccatgagg agaacacttg gattaaccgc taaggcgcat gccaaggtga cacgttgttg
    49141 ttgtccacca ctgagtttat cgggctttcg atccgcatat tgagcaattt gcatgagttc
    49201 aagatacttg ttggtctgtt gaatcaattc ttcttttgga accttctttt gcataagacc
    49261 aaaagcaacg ttgtctcgga cagtcaaatg tgggaaaata gcgtagtttt ggaaaaccat
    49321 cccgatattg cgtttgctgg gttccatatt attgattttt gtatcatcga agtaaaattc
    49381 tccaccttcg atactgttga aacctgcaat catacgaaga agggtcgttt tcccacatcc
    49441 tgaagctcca agaagggtaa agagacttcc ttttggaatt gtaatgttca aattctcaat
    49501 aacagggaca tcgtggtaga tttttttggc gttaataatt ttgatctcac tcatagtgaa
    49561 cctcttttac tgtttagatt ggatatctgt aaagacttcg ttgtatttct taacgatatc
    49621 tgatttattc ttgatgacat aatcataatc ttcagtgagt gttttgattt tgtcaattgg
    49681 tttcatgttt tcgcttgttt tagcattttt acgaacagga cggttagtag tggttgtacc
    49741 aagtgtatct tgtacttctt gagagataat aaaatcgata aatttcttgg cattttccat
    49801 atttttagat tttttaacga tagcagcact agcaggtagg aagacggttc cttcttttgg
    49861 atagactacc ttaatgttag ctccgtcatt taagagttta actgctggat cttcataaga
    49921 gagaccaaca gccatttctc catcagcgac tactttatag acactagatg aacttgaacc
    49981 gattttacca tcaataagtg tgaaaagatc ttttacataa gaccaagcct tatcatcttt
    50041 gtaaccacct tgagcttgta gcatatttgt taattgagca aaggcgctag aagagtttgc
    50101 tgggtcagca gttgcgattt ttccttttag ttcaggtttg aaaagatcgt tatatccttc
    50161 gatgttcatg cctttagtta aatcagggtt gacgattaaa acactaccat ctagtgtata
    50221 aggagtagag tagccagttg tgttttgata ttctttgata acattatcat tttcttttga
    50281 agtatagttt tcaaagagtt ctccgtgggt agtatattgt gtataagaac caccaaagat
    50341 aacatcagct acaggaactt ctttttctga ctctagtttt ttgaaaagtt ctccagtacc
    50401 agcttgaatc agttctactt tgataccata tttttcttca aaggcaggaa tagttgctcc
    50461 aattaagccc tctgagtttg gtgaataaac gactagcgaa ccgccgtctc ctttatcaga
    50521 tgaactgtca tcggcagatt cattagaaga acaagcagca taatacatcc atttcttttt
    50581 catgatggat acctccgttg tgttatttaa gtttatttta aaacaatgta agcgttttta
    50641 aaacatacaa ttctattcta tagtgtattg aatctataac agtacac
//
LOCUS       DAVKNL010000009        49187 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVKNL010000009 DAVKNL010000000
VERSION     DAVKNL010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 49187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49187
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.009"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            <1..749
                     /locus_tag="VW411_000471"
     CDS             <1..749
                     /locus_tag="VW411_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU9793264.1"
                     /translation="QDFNNLNIITVLKGRTQAVIRNHFLKYDRAVRCRVKIITMDMFS
                     PYYDLARQLFPCAKIVLDRFHIVQHLSRAMSRVRVQIMNQFHRKSHEYKAIKRYWKLI
                     QQDSRKLSDKRFYRPTFRMHLTNKEILDKLLSYSQDLKHHYQLYQLLLFHFQNKKPEK
                     FFGLIEDNLKQVHPIFQTVFKTFLKDKEKIVNALQLHYSNAKLEATNNLIKLIKRNAF
                     GFRNFENFKKRIFIALNIKKERTKFVLSRA"
     gene            complement(821..1471)
                     /locus_tag="VW411_000472"
     CDS             complement(821..1471)
                     /locus_tag="VW411_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEU9793265.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            1583..2425
                     /locus_tag="VW411_000473"
     CDS             1583..2425
                     /locus_tag="VW411_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU9793266.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQIGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            2501..2938
                     /locus_tag="VW411_000474"
     CDS             2501..2938
                     /locus_tag="VW411_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU9793267.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            3006..3308
                     /locus_tag="VW411_000475"
     CDS             3006..3308
                     /locus_tag="VW411_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793268.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYIFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(3399..4472)
                     /locus_tag="VW411_000476"
     CDS             complement(3399..4472)
                     /locus_tag="VW411_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEU9793269.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPRDPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILDAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            4628..5305
                     /locus_tag="VW411_000477"
     CDS             4628..5305
                     /locus_tag="VW411_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9793270.1"
                     /translation="MHKILLIEDDQVIRQQIGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            5298..6272
                     /locus_tag="VW411_000478"
     CDS             5298..6272
                     /locus_tag="VW411_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU9793271.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLVLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(6321..8264)
                     /gene="thrS"
                     /locus_tag="VW411_000479"
     CDS             complement(6321..8264)
                     /gene="thrS"
                     /locus_tag="VW411_000479"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU9793272.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELVSGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(8322..8645)
                     /locus_tag="VW411_000480"
     CDS             complement(8322..8645)
                     /locus_tag="VW411_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEU9793273.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDSKWTVEEDLLALSNDIGN
                     FQRLVMTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENFLSDKEKQLN
                     VRTWK"
     gene            complement(9076..9276)
                     /locus_tag="VW411_000481"
     CDS             complement(9076..9276)
                     /locus_tag="VW411_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793274.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKGLYINNLL
                     FFTPLVILIVSLLYSYRFRFSL"
     gene            9513..10142
                     /locus_tag="VW411_000482"
     CDS             9513..10142
                     /locus_tag="VW411_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU9793275.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTPDFPEEFKPLAKAQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
     gene            10283..10552
                     /gene="rpsO"
                     /locus_tag="VW411_000483"
     CDS             10283..10552
                     /gene="rpsO"
                     /locus_tag="VW411_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU9793276.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            11368..11982
                     /locus_tag="VW411_000484"
     CDS             11368..11982
                     /locus_tag="VW411_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEU9793277.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(12274..13023)
                     /locus_tag="VW411_000485"
     CDS             complement(12274..13023)
                     /locus_tag="VW411_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEU9793278.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            13156..15492
                     /locus_tag="VW411_000486"
     CDS             13156..15492
                     /locus_tag="VW411_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEU9793279.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEQNFLRKSMLRALPSALMVVFSVLFVKMF
                     GASQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTEQTLPVYGVMMLVFTVIFILTSRYQAKK"
     gene            complement(15623..16096)
                     /gene="tnpA"
                     /locus_tag="VW411_000487"
     CDS             complement(15623..16096)
                     /gene="tnpA"
                     /locus_tag="VW411_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISSpn6 family
                     transposase"
                     /protein_id="HEU9793280.1"
                     /translation="MREDNQSLSHTTWNCKYHIVFAPKYRRQIIYGRYKASIGRIIRD
                     LCERKGVIIHEANACSDHIHMLISIPPKLSVSSFMGYLKGKSSLMIFDKHANLKYKYG
                     NRKFWCRGYYVDTVGRNQKVIAEYIQNQLQEDRVADQLTLFESVDPFTGEINKRK"
     gene            16760..18223
                     /locus_tag="VW411_000488"
     CDS             16760..18223
                     /locus_tag="VW411_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEU9793281.1"
                     /translation="MSRKQEQMETLLLLLRDSKDYISAKVLGEKLNCSDKTVYRLVKG
                     INKDCPVEAFILSEKGRGFKLNPRSSLVDVDGNFTEAFDPEVRREKLLERLLLTAPKP
                     HSIYDLGEEFYVSESVVLKDRQILQESLAIYGLDLKMRQRKLFIDGDEAQIRSAILNL
                     LPMFNQLDLEQITQNKVQPLDGELAHFCLGLLITLERELGVNIPYPYNINIFSHLYIF
                     ISRNRRSTSIHVVAPSKPTIVDEKIYSVCQKIIQEIEQYFRMKVDAVEIDYLYQYVVS
                     SRLQKPFSSGKLPFSQRVLDVTHYYFSRMCMDNREIETTDPDFVDLASHISPLLRRLD
                     NRVQIKNSLLSQILLTYPNLVKELTTISKEVSLVFGFASLSLDEIGFLVLYFARFQEK
                     RARPLKTVVMCTSGVGTSELLRARLEKQFSELDIIDVVAYHQLDELINLYPDLDFIVT
                     TVALQEPASVPFVLVSVFLTEGDKQRLQAKIQEINYE"
     gene            18216..18644
                     /locus_tag="VW411_000489"
     CDS             18216..18644
                     /locus_tag="VW411_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9793282.1"
                     /translation="MNNLSLVLMDISVQNRQEAYKELANQISLLVSEDTEKIEELLYY
                     RERQGSIEVAKGVLLPHCEGNFQHHVLVITRLKSPIREWSKDIQCVDLIIGLAIAVSQ
                     DKSCIKTLMRRLADESFINQLKQLTKEELREIIYGNQRYS"
     gene            18622..19071
                     /locus_tag="VW411_000490"
     CDS             18622..19071
                     /locus_tag="VW411_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9793283.1"
                     /translation="MEIKDILNVSLIQTDLQMQSKEEVFEALAQLLVETGYVSDRDQF
                     IEGLYQREAEGQTGIGNYIAIPHSKSSAVEKAGVVIAINHNEIPWETIDGKGVKVIVL
                     FAVGDDTEAAREHLKTLSLFARKLGNDEVVAKLVRAQTSDDVIAAFC"
     gene            19099..19410
                     /locus_tag="VW411_000491"
     CDS             19099..19410
                     /locus_tag="VW411_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEU9793284.1"
                     /translation="MKIVGVAACTVGIAHTYIAQEKLENAAKVAGHVIHVETQGTIGV
                     ENELSQEQIDAADVVILAVDVKISGMERFEGKKIIKVPTEVAVKSPNKLIAKAVEIVT
                     K"
     gene            19436..20521
                     /locus_tag="VW411_000492"
     CDS             19436..20521
                     /locus_tag="VW411_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEU9793285.1"
                     /translation="MLKHLNLKGHLLTAISYMIPIVCGAGFLVAIGLAMGGGVPDALV
                     AGKFTIWDALATMGGKALGLLPVVIATGLSYSIAGKPGIAPGFVVGLIANSVGSGFIG
                     GILGGYIAGFLVQAIIKKVKVPNWIKGLMPTLIIPFVASLVSSLIMIYIIGAPIAAFT
                     NWLTSLLQSLGSASNGLMGAVIGILSAVDFGGPLNKTVYAFVLTLQAEGVKEPLTALQ
                     LVNTATPVGFGLAYFIAKLLKKNIYTQEEIETLKSAVPMGIVNIVEGVIPIVMNNLVP
                     GLIATGIGGAVGGAVSLTMGADSAVPFGGVLMLPTMTRPVAGICALLANIVVTGLVYA
                     ILKKPIKHAEPVMTVEEEIDLSDIEIL"
     gene            20532..21227
                     /locus_tag="VW411_000493"
     CDS             20532..21227
                     /locus_tag="VW411_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU9793286.1"
                     /translation="MSRIEFSPSLMTMDLDKFKEQITFLNDKVASYHIDIMDGHFVPN
                     ITLSPWFIQEVQKISDTPLSVHLMVTDPTFWVDQVLDLQCEYICIHAEVLNGLAFRLI
                     DKIHDAGLKAGVVLNPETPVSTIFPYIDLLDKATIMTVDPGFAGQRFLESTLYKIQEL
                     RQLRVQNGYHYIIEMDGSSSRKTFKQIDVAGPDIYVIGRSGLFGLDDDIAKAWDICSR
                     DYEEMTGKTMPIK"
     gene            21264..23231
                     /gene="tkt"
                     /locus_tag="VW411_000494"
     CDS             21264..23231
                     /gene="tkt"
                     /locus_tag="VW411_000494"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU9793287.1"
                     /translation="MSLQSVNAIRFLGVDAINKSNSGHPGIVMGAAPMAYSLFTKHLR
                     ITPEQPNWINRDRFILSAGHGSMLLYALLHLTGYKDVSMDEIKNFRQWGSKTPGHPEV
                     THTSGVDATSGPLGQGISTAVGFAQAERFLAAKYNKDGFPIFDHYTYVIAGDGDFMEG
                     VSAEAASYAGHQALDKLIVLYDSNDICLDGETKDTFSENVRVRYDAYGWHTVLVEDGT
                     DLAAISTAIETAKFSGKPSLIEVKTVIGYGSPNKSGTNAVHGAPLGAEETGATRKFLG
                     WDYDPFEVPEEVYSDFKTNVADRGQEAYDAWASLVSDYKVAYPEVASEIDAIVAGKSP
                     VTITEKDFPVYENGFSQATRNSSQDAINTAAVLPTFLGGSADLAHSNMTYIKADGLQD
                     KYNPLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAIRLSAIQELP
                     VTYVFTHDSIAVGEDGPTHEPVEHLAGLRSMPNLTVIRPADARETQAAWHHALTSTTT
                     PTVIVLTRQNLVVEEGTDFGKVAKGAYVVYDTPGFDTIIIATGSEVNLAIKAAKELVL
                     QGGKVRVVSMPSTELFDAQDATYKEDILPSKTRRRVAIEMAATQSWYKYVGLDGAVIG
                     IDIFGASAPAQTVIDNYGFTVENIVAQVKSL"
     gene            23606..24877
                     /locus_tag="VW411_000495"
                     /pseudo
     CDS             23606..24877
                     /locus_tag="VW411_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(24951..25481)
                     /locus_tag="VW411_000496"
     CDS             complement(24951..25481)
                     /locus_tag="VW411_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000883388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HEU9793288.1"
                     /translation="MLDEKRKNYVEDKRQNFPSLPSWKMDLFSEEEKRIYFQTTCEVS
                     SKDSAINKYKMEKIIKRSNKGNVYRAIRKSDGQKVIIKQSRPFVNYDAEGEWTALDDI
                     KNEAHMLKKLADKSYTTNLTDEFYIVDDYFLVQEQVDGLNFEEFIRETEHSLNIREKT
                     LDNIVNIVSYIHKLGI"
     gene            complement(25475..25943)
                     /locus_tag="VW411_000497"
                     /pseudo
     CDS             complement(25475..25943)
                     /locus_tag="VW411_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847907.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            26492..27169
                     /locus_tag="VW411_000498"
     CDS             26492..27169
                     /locus_tag="VW411_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEU9793289.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            27156..27953
                     /locus_tag="VW411_000499"
     CDS             27156..27953
                     /locus_tag="VW411_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEU9793290.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQEDDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVIFVEKIAALLSQWKEDKGWSIDILPSQASTNPFHHI"
     gene            27970..29073
                     /locus_tag="VW411_000500"
     CDS             27970..29073
                     /locus_tag="VW411_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU9793291.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQEIDFYQRSGVFLLKKDESNLE
                     ELYQLALQRREESPLIGQLAILNQASANELFPGLQGFDRLLYASGGARVDGQLLVTRL
                     LEVSHVKLVKEKVTLTPLASGYQIGEEEFEQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDETLLQQME
                     EATLTHYLILAEATSKSERVGIRAYTSDFSPFFGQVPDLTGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPLNYPIENYVKRVKSE"
     gene            29074..29154
                     /locus_tag="VW411_000501"
                     /pseudo
     CDS             29074..29154
                     /locus_tag="VW411_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            29374..30393
                     /gene="galE"
                     /locus_tag="VW411_000502"
     CDS             29374..30393
                     /gene="galE"
                     /locus_tag="VW411_000502"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9793292.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVEGITGVEIPFYEADIRDTDTLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            30395..31360
                     /locus_tag="VW411_000503"
     CDS             30395..31360
                     /locus_tag="VW411_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9793293.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKNKVKRL"
     gene            complement(31411..31617)
                     /locus_tag="VW411_000504"
     CDS             complement(31411..31617)
                     /locus_tag="VW411_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEU9793294.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDILEAVKNCPTRALIGNQEA"
     gene            31669..32145
                     /locus_tag="VW411_000505"
     CDS             31669..32145
                     /locus_tag="VW411_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEU9793295.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKGFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            32154..32825
                     /locus_tag="VW411_000506"
     CDS             32154..32825
                     /locus_tag="VW411_000506"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEU9793296.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPQVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            32956..33294
                     /locus_tag="VW411_000507"
     CDS             32956..33294
                     /locus_tag="VW411_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEU9793297.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLADGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            33297..33926
                     /locus_tag="VW411_000508"
     CDS             33297..33926
                     /locus_tag="VW411_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEU9793298.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITKHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            34166..35317
                     /locus_tag="VW411_000509"
     CDS             34166..35317
                     /locus_tag="VW411_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9793299.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            35635..36384
                     /gene="truA"
                     /locus_tag="VW411_000510"
     CDS             35635..36384
                     /gene="truA"
                     /locus_tag="VW411_000510"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEU9793300.1"
                     /translation="MTRYKATISYDGYAFAGFQRQPHARSVQEEIEKTLTRLNKGQAI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFTVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            36374..37147
                     /locus_tag="VW411_000511"
     CDS             36374..37147
                     /locus_tag="VW411_000511"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU9793301.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LVKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            37125..37586
                     /locus_tag="VW411_000512"
     CDS             37125..37586
                     /locus_tag="VW411_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9793302.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSALMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
     gene            37814..39154
                     /locus_tag="VW411_000513"
                     /pseudo
     CDS             37814..39154
                     /locus_tag="VW411_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(39282..40304)
                     /gene="cbpF"
                     /locus_tag="VW411_000514"
     CDS             complement(39282..40304)
                     /gene="cbpF"
                     /locus_tag="VW411_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpF"
                     /protein_id="HEU9793303.1"
                     /translation="MKLLKKMMQIALATFFFGLLATNTVFADDSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDPLSGEMVVGWQYIPAPHKGVTIGPSPRIEIALRPDWF
                     YFGQDGVLQEFVGKQVLEAKTATNTNKHHGEEYDSQAEKRVYYFEDQRSYHTLKTGWI
                     YEEGHWYYLQKDGGFDSRINRLTVGELARGWVKDYPLTYDEEKLKAAPWYYLNPATGI
                     MQTGWQYLGNRWYYLHSSGAMATGWYKEGSTWYYLDAENGDMRTGWQNLGNKWYYLRS
                     SGAMATGWYQESSTWYYLNASNGDMKTGWFQVNGNWYYAYDSGALAVNTTVGGYYLNY
                     NGEWVK"
     gene            complement(40323..41144)
                     /gene="cbpG"
                     /locus_tag="VW411_000515"
     CDS             complement(40323..41144)
                     /gene="cbpG"
                     /locus_tag="VW411_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpG"
                     /protein_id="HEU9793304.1"
                     /translation="MNVEGRGSANFIKDNVLITAAHNYYRHDYGKEADDIYVLPAVSP
                     SQEPFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLGTLGLPTSQKNLT
                     GITVTITGYPSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSGSTVYDASHRVVG
                     VHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGWKKINGSWYHYRQHDKQTGWQE
                     INDTWYYLDSSGKMLTDWQKVNGKWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            complement(41225..41464)
                     /locus_tag="VW411_000516"
     CDS             complement(41225..41464)
                     /locus_tag="VW411_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793305.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPESNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(41614..41880)
                     /locus_tag="VW411_000517"
                     /pseudo
     CDS             complement(41614..41880)
                     /locus_tag="VW411_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(41905..42159)
                     /locus_tag="VW411_000518"
     CDS             complement(41905..42159)
                     /locus_tag="VW411_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEU9793306.1"
                     /translation="MAANYLKAMQSYLKETDLPKLEQLVVTKSLWDTVDILDRVVGSL
                     VYEHPELEEIILILFENLFKPRQLYLQPQNSVLSNLRLAS"
     gene            complement(42141..42382)
                     /locus_tag="VW411_000519"
                     /pseudo
     CDS             complement(42141..42382)
                     /locus_tag="VW411_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(42383..43015)
                     /locus_tag="VW411_000520"
     CDS             complement(42383..43015)
                     /locus_tag="VW411_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9793307.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQEEF"
     gene            complement(43029..44024)
                     /locus_tag="VW411_000521"
     CDS             complement(43029..44024)
                     /locus_tag="VW411_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9793308.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSMTCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKS
                     LSGKLNLLTEALQKAENQSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(44021..44719)
                     /locus_tag="VW411_000522"
     CDS             complement(44021..44719)
                     /locus_tag="VW411_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEU9793309.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLIFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(44796..45806)
                     /locus_tag="VW411_000523"
     CDS             complement(44796..45806)
                     /locus_tag="VW411_000523"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEU9793310.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(45790..46797)
                     /locus_tag="VW411_000524"
     CDS             complement(45790..46797)
                     /locus_tag="VW411_000524"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEU9793311.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLEICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYDVSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFSISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLTSSKETVTSLVEALEQGKSEKIIDQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQTVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(46784..47737)
                     /gene="mvaD"
                     /locus_tag="VW411_000525"
     CDS             complement(46784..47737)
                     /gene="mvaD"
                     /locus_tag="VW411_000525"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEU9793312.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMAFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGHRYRLIVSKTKDLSQDDCC"
     gene            complement(47719..48597)
                     /gene="mvk"
                     /locus_tag="VW411_000526"
     CDS             complement(47719..48597)
                     /gene="mvk"
                     /locus_tag="VW411_000526"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEU9793313.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLNITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(48772..49050)
                     /locus_tag="VW411_000527"
     CDS             complement(48772..49050)
                     /locus_tag="VW411_000527"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9793314.1"
                     /translation="MIRKAYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMLPHDFPLYLMVWSFFRRSMTTGWFQVNGRWYYAYSAQVPWQ"
BASE COUNT        14361 a         9517 c        10381 g        14928 t
ORIGIN      
        1 cgcaagattt taacaatctc aatatcatca ctgttcttaa gggaagaaca caagctgtca
       61 tccgaaatca ctttcttaaa tatgatagag ccgtccgatg tcgcgtcaaa atcattacta
      121 tggatatgtt tagtccttac tatgatttag ctagacaact tttcccgtgt gctaaaatcg
      181 tgttggatcg ctttcacatt gtacaacatc ttagccgtgc tatgagtcgt gtgcgtgttc
      241 aaatcatgaa tcagtttcat cgaaaatccc atgaatacaa ggctatcaag cgctactgga
      301 agctcataca acaggatagc cgtaaactga gtgataagcg attttatcgt cctacttttc
      361 gtatgcactt aaccaataaa gagattttag acaagctttt gagctattca caagacttga
      421 aacaccacta tcagctctat caactcttgc tgtttcactt tcagaataag aaaccggaga
      481 aatttttcgg actcattgag gacaatctaa agcaggttca tcctattttt cagactgtct
      541 ttaaaacctt cctcaaagat aaagaaaaga ttgtcaacgc ccttcaacta cactattcta
      601 atgccaaact ggaagcgacc aataatctca tcaaacttat caagcgcaat gcctttggtt
      661 ttcggaactt tgaaaacttc aaaaaacgga tttttatcgc tctgaatatc aaaaaagaaa
      721 ggacaaaatt tgtcctttct cgagcttagc tgacttcaac ccactacagt tgacaaagag
      781 ccaaaataaa actttcaggg ttggtagggg acttgagaaa ttagttgatt ttctcgacat
      841 agagttgttt tgcaatgtcc atattcactt gtaaatcctc gtcgttactg aggatagtga
      901 aggtattgct gaagccatca aactgcttga cttggagctg gtcaccgatg tgaagtgagt
      961 gcttgtccag ataatggaga atgtcaaaac tatcgtgcac ccgagtcagg cggtaggcgc
     1021 cagcttcctt gatatcagct agtgggaggt tattgatttc aacgagtagt tctcccttgg
     1081 caggaatagt tcccccgtgg gggcaggttt tagggaaacc tagcagttta tctagtcttt
     1141 ccacgaacag gtcagagaca gtgtgttcca agacctcagc ttcctcgtga atctggtcac
     1201 ttgtatagtc taaatgatga actagaaaaa cttcaatcaa gcggtgctta cgatagagct
     1261 cagagaccag tttgagaccg aggtcagtca gtagatagcc acattccttg tcctttagga
     1321 tgagattttc acttttcatt cgtttgatca tttcagttac ggcaggggga gagacttgca
     1381 tgcgagccgc aatttccttg ttggtaatct tatgcaggtc tatgccaatt tcataaatac
     1441 attttagata gtcttctttg tttggggtca ttcgtttcct cttgtctaat atctttatct
     1501 agtatatcaa aaaaagctag atttctctag ctgtgacttt ttatgttata cttattgcaa
     1561 gagaaaaaac gaggagatgg atatgacgaa aatagctctt ctttcagata ttcatggaaa
     1621 taccaccgcc ttggaggctg ttttggcaga tgctcggcag ataggagtgg atgaatactg
     1681 gcttttggga gatattctca tgccagggac agggcgtaga aggattttgg acttgttgga
     1741 tcaactaccg attacggcta gagttttggg aaactgggaa gacagtcttt ggcatggtgt
     1801 ccgtaaggaa ttggacagta ctcgccccag tcaacgctat ctcttgcgcc agtgccagta
     1861 tgttttagag gaaatttccc tagaagaaat tgaagtgctc cacaatcaac ctcttcaaat
     1921 tcatcgtcag tttggggatt tgacggtggg aattagccat catctgcctg ataagaactg
     1981 ggggcgagag ttgattcata ctggcaaaca agaggagttt gaccgcttgg tgactcatcc
     2041 tccctgtgat attgctgttt atggacatat tcaccagcag ttgcttcgtt acgggactgg
     2101 tgggcaattg attgtcaatc cgggttcgat tggccaacct ttctttttgg acgctcagct
     2161 acggaaggac ttgcgggccc agtatatgat tttggagttt gatgacaaag gcctggtaga
     2221 tatggacttc cgacgggtag actacgatgt agcagctgaa ttgcagctgg ctaaagacct
     2281 gagacttccc tattttgagg tttactatga aagtctggtc aatgggatcc accatactca
     2341 tcatcaggaa tttttgagag aattggctca gaaagagggc tgcgatcggg agttagacga
     2401 ctggttgaaa agtggtaacg attgacatta caactaaaaa gggtataata atagaaaaag
     2461 aagagtagag gcagggtagc ctcgtaaaaa ggagaagagg atgcaaattc caagtagatt
     2521 taccattgcg actcatatgc tgataatcat tgccctcgag gggaaggaaa gcaaggtgac
     2581 cagtgatttt ctggctgcta gtgtcggggt caatcctgtc attatcagaa agatcttgtc
     2641 ccagttgaag aaggcagagc tgatttcagt agcgcgtgga acgggcggaa cagagattgt
     2701 caaggacctt aaggatatta gtcttttaga tgtttatcag gcggtcgaat gtcttggtaa
     2761 gacaggtcaa ctcttcagtt tccatgacaa tccgaatcca aattgccctg taggagctca
     2821 tattcatgat gttttggatc aaaaattgga gagaattcag ttgactatgg aggcagaact
     2881 tggtcaaacc agtctagaaa aagtcgtggc cgatgcagag agtcagatga aggattaaga
     2941 gggataaatg ccctcttttt tctttggata attatgataa aatgtagcta ttaaaggagt
     3001 aagaaatgta tctcgttata ggaattgtat tagcctttat agtgtcattt tggaaggata
     3061 atagaagttt gtggaatcca gtattatttt tattatccct catttcaagt tatttttatc
     3121 tatcctacct tttttataaa aatggatacg agaatgttca gttagctttt tatatatttg
     3181 cctttgtctt acttcctttt ttgctttttc taagtggcat ctttttaatc tataatgggg
     3241 tcattttgtt gaagcgagag ggacgttcca agccccatta tctctcaatg ctctttgatt
     3301 tttattgact attagattct atttaaaaag acttagaatg cacatgaaag tatgtattct
     3361 aggtcttttg ttttgtatac atttaatttt caagagtatt atctattttt ccatgcctgg
     3421 taacgagtat caatcaataa ctgggtaagg cgacccaagg tttctctttc ttctggagtg
     3481 agggattgag cttggacaaa gagatgatga atgtattcaa gcgcctttaa ggaagccaag
     3541 tcattgatag agctaatacc agcatcaaga atagtgccaa agaagaggtc gaagtagagc
     3601 tgaagttctt cgtcagggac tgtggccacc cattctttaa aggttgtgtc tacttgctgg
     3661 ctatcactgt tggtcttatc cagttggacg aagtgcttgt cctcaatctg ccaactaaag
     3721 gtatcgtgct gggcgatgcc acccagggca gtactctgaa cgatgatttg gtgagcagga
     3781 atttccagca tcataccgat aatggaacct tgtggaatga atatcttgct tctatccatt
     3841 atcctttgat aacccgcagt ctgtgtcaat tcttgatgga gaccaggtgc atcaaatgta
     3901 taaactgctg tgatctgatt ttgcaaactt tgctcaattt ggctagcagc atagatagcg
     3961 agatttcctc ccttggaatg cccagctaga ataacctttt gcttaggatg atgggcaaaa
     4021 aagttcttta aatagcgaag ggcgtgcttt tgagcaggaa tttccttcat ataggtcagg
     4081 tggaaatctt ccttccagcc aatgatactg tcatctgtcc cacgaaagac aatcagatag
     4141 gtatcgaggc tgacacgata agtcatagcc gcaaattgct tttgcagttc agggtcgatg
     4201 tcgttgataa aatgggagag tttgcaattt ttgaagcgct tgtgttgagc caattcatct
     4261 aataattgaa ggcgattttt gctagtaagc atggtgggat ctcttggaac ctgaggtgct
     4321 agatctaaaa gacgttgagg aagtgtggag accagattat caaaggagag gtaggtgatt
     4381 tctattaagg ttagaatgtc taactcattc aagggaaggt cgtaataaga atcatatgcg
     4441 acatctttca gatagtcaaa aatattggcc ataagagctc ctttcttttt atttatctta
     4501 gcaaatttcc ctcaaattag ctagtagcat agcctgtttg tactggctaa aaacaggcta
     4561 tttcaaattc agtttcagac catctagcat ggaaaaatct gttataataa tggaaaagga
     4621 gaagcgcatg cacaagattt tattaataga agatgatcag gtcattcgtc aacagattgg
     4681 gaaaatgctc tctgaatggg gatttgaagt ggtcctggta gaagacttta tggaagtttt
     4741 gagtctattt gttcagtcgg aacctcatct ggtcctcatg gatattggtt tgcccttgtt
     4801 taatggttat cactggtgtc aggaaatccg caagatttcc aaggtaccta tcatgtttct
     4861 ttcttcgaga gaccaggcta tggatattgt catggcaatc aatatggggg cggatgactt
     4921 tgtgaccaag ccttttgacc agcaggttct tttagctaag gttcagggct tgttgcgtcg
     4981 ttcctatgag tttgggcgtg atgagagttt gctggaatat gctggtgtta tcctcaatac
     5041 caaatccatg gatttacatt atcaagggca agtcttgaat ttgaccaaga atgaattcca
     5101 gattttacgc gtgttatttg agcatgcagg caacatcgta gcacgtgacg acctgatgcg
     5161 ggaactttgg aacagtgact ttttcattga tgataatacc ctctctgtca atgtggctcg
     5221 tttgcgtaaa aagttggagg agcagggatt ggtaggattt atcgagacca agaaaggaat
     5281 agggtacgga ttgaagcatg cttgattgga aacaattttt tctagcctat ctgcgctccc
     5341 gtagtcgtct ttttatctat ctgctttctt tggcatttct tgtcttactc tttcagtttt
     5401 tatttgccag tctaggaatt tacttcctct actttttctt cttgtgttgc tttgtaacca
     5461 tattattttt cacttgggac atattggtgg aaacgcaggt ctatcgccag gaacttctct
     5521 atggagagag ggaagccaag tctcctttgg aaatagcttt agcagaaaaa ttagaagcgc
     5581 gtgagatgga actctatcag cagaggtcaa aagcagaaag aaaactgacg gatttgctgg
     5641 attactatac cttgtgggtc catcagataa agacccccat tgcagccagt caactcttag
     5701 ttgcagaagt ggtcgaccgc caactgaagc agcagctaga acaggaaatt ttcaaaatcg
     5761 actcctatac caacctagtt ttacagtacc tgcgtttaga aagtttccat gatgatttgg
     5821 tcttaaagca ggttcaaatt gaggacttgg tcaaggaaat aattcgtaaa tatgctcttt
     5881 tctttattca aaaaggctta aatgtcaatc tacatgacct tgataaagaa atcgtgacgg
     5941 ataaaaagtg gctgctagtg gttattgagc aaatcatctc aaacagtctc aagtacacca
     6001 aggaaggtgg tctggagatt tatatggatg accaagagct ttgtatcaaa gatacgggaa
     6061 tcgggataaa aaacagtgat gtcctccgag tatttgaacg tggcttttca ggatacaatg
     6121 gccgtttgac ccagcagtcc tctggacttg gcctttatct atctaagaaa atttctgaag
     6181 aactggggca ccagattcgt atcgagtctg aggtcggaaa aggaacgaca gtgcggattc
     6241 agtttgctca agtgaactta gtccttgagt aaaaatagat gagaaaaagc ctccagattg
     6301 gaggctttta tgctagactc ttatttctca acgcgtgatt tgttggcgat atcagctagg
     6361 atagcttgaa caaaattatc aactgagaca gtttgtgttt ctttttggcc gtagcgacga
     6421 acgttgactg tttcgtcttc catttctttg tctccaacaa ttaattggta aggaatcttg
     6481 ctggtttgtg aagcacggat cttgaactgc attttttcat tgcgctcatc tacgtctgca
     6541 cggacaccgc ggtcacggag tttcttggcc acttcccaag cgtagtccac gtgtttttcg
     6601 ttagatactg ggatgagggt tacttggtgt ggtgccagcc atgttgggaa ggcccccttg
     6661 tagttctcaa tcaagatagc tgtgaagcgt tccatagttg agataacccc acggtggatc
     6721 atgactggac ggtgatcttc gccatcagct ccgatgtatt tgaggtcgaa gcgttctggc
     6781 aacaagaaat caagttggat agtagaaagg gtttcttctt ttccaagggc agttttaatc
     6841 tggatatcca attttggtcc gtagaaggct gcttcacctt cggcttcaaa gtagtccacg
     6901 cccatttcat caagagctgc acgaagcatg gtttgggcat tttcccacat ctcatcgtta
     6961 tcaaagtact tatgagtatc ttgagggtca cgaagagaga ggcggaagcg gtagtcagtc
     7021 aagttgaagt cttcataaac atcgataatc aactgaaggg cacgttggaa ttcttcttgg
     7081 atttgttctg gagtaacgaa taggtgaccg tcgttgagtg acatttcacg tacacgttga
     7141 aggccagtga gggcaccaga tttttcgtaa cggtgcatca taccgatttc agcgatacgg
     7201 attggcaatt cacggtaaga gtgaacatgg tgtttgaaaa cttggatgtg gtgcggacag
     7261 ttcattggac gaaggacaaa ttcttccccg tcacccatgt ccatggttgg gaacatgtct
     7321 tcttggtaat gatcccagtg accagaagtc ttgtaaagct caacagaagc aagtggtgga
     7381 gtgtagacgt gttggtagcc agaaaccaac tctttgttta cgatgtagcg ttccaattca
     7441 cgacggatag tcgcaccatt tggcaaccag aatggcaaac cttgtcccac ttcttgtgaa
     7501 atcataaaga ggtcaagctc tttaccaagt ttacggtggt cacgttcctt agcttcttca
     7561 cgcatttgaa ggtagttttt caagtctttc ttgtcaaacc aagctgtacc gtagatacgt
     7621 tgcatcatag cgttgtcgct gtttccacgc cagtacgcac cagctacatg gagaaggtgg
     7681 aagatttgga tacgacctgt tgatggaacg tgaggtccac ggcagaggtc tacatattca
     7741 ccctgacgat agatagtcaa accgccttcg tcttctgagt gttcttcaat caattccaac
     7801 ttgtaagggt catttttgaa gatttcacgt gcctcgtctt tagtcacttc ttcacgaata
     7861 gatgggaagt tttctttgac gattttttgc atttcttctt cgatacgagg aaggtcttcg
     7921 ttagagattt gaccagctgt gttgtcagta tcgtagtaga aaccatcttc gatggctgga
     7981 ccaactccca agtgaatgtc tgggaaaaga cgacgagctg cttgggcgaa caagtgagct
     8041 gctgagtgac gcaagattgg aagggcatct tcgtgatcag gtgtcacaat ttcgatgctt
     8101 ccatcttcag tgatagcgcg agtagtgtcg atgagtttgc cgttgaattt accagccaag
     8161 gcttttttag ctagggaatt gctgatagat tgggcaattt caaaagttgt tacgccagat
     8221 tcgaattcac gaacagcgcc atctgggaaa gtaatgttaa tcatgatgtt ctcctttttt
     8281 attatttcat agtttctaag cattgatttt ttatcagcag actacttcca agtcctaacg
     8341 ttcaattgct tttctttatc agagaggaag ttttccattt ccgtcagaat gtctatatcc
     8401 aaacgttgag aaagttctaa tagccaccag atattttctg aaagtttttg ttccagtgtg
     8461 tagggtgttt catcatagta gcgtccttgc tttgtcatca ccagtcgttg gaaatttcca
     8521 atatcattag ataaagccaa gaggtcttct tctaccgtcc acttggaatc atgatgctta
     8581 acttccagtt cgtgataagc ttgtcggatt gcccaactgc gctccactaa ttcttgcaac
     8641 tccataatta gtatcctaat aaaaaaatgc gacatcctcg aaaggacgtc gcaacgtggt
     8701 tccaccttca tttatgtacc tcaaaagaga gggacacctc tgatcggctc taacgtggcc
     8761 accgttttat gttttcataa aaaactcaag agtagtatca gtttagactt cctatgcgtt
     8821 tccagcaacc acgcactctc tagtagaaag ggactaagtg acttgtctct atggattatt
     8881 atagcatgat tgtgagattt ttcaaggatt ttgtgaaagt tttttgtttt tcattttttg
     8941 tagaatatgc ttgatgctaa taccagaatc catgccgacc agagcaactg tttcatagat
     9001 tagcaagtaa attagtataa actcaccaaa ggttatgata gtaaagtaaa agagcagtcc
     9061 gttaccaact aaccatcaaa gtgagaaacg gaaacggtag ctatagagta acgatacaat
     9121 caagattacc agaggtgtaa agaagagaag attgtttata tagagtccct tgagtgtaag
     9181 ggggtcagaa acaactagta agaacaactc ataaagtggc caataaaaga ataatatgac
     9241 cagatagtag gggagatggg aatagaatct acgcatgata atcacctcgt tttaaaaaaa
     9301 cagacaactt agatctcttg ctatgattat tatagcatgt ttaaaacaag aaagtaaatt
     9361 tgttgacaaa gaagattttt tttcactaga atcatggtta gttttcttgg tagaaatatt
     9421 ttgataataa ttaagcaaga ctattgacat tgattattaa gagtgtcaca atttctgtaa
     9481 ctaaaataat tacaacaaga gaggtataaa aaatgaaact agcagttatt gcagcaaatg
     9541 gacaagcggg gaaagcaatt gttgaagaag cagtcaaacg aggacatgaa gtaacggcta
     9601 ttgttcgttc tgaaaacaaa agtcaagctg agtctattat taaaaaagat ttatttgagt
     9661 tgacaaaaga tgatcttact ggatttgatg ctgttatttc agcgtttggt gcttatacac
     9721 cagatacgct accgctccac agcaaatcaa tcgaactatt taatcagctt cttgctggaa
     9781 cacagacacg ttttctagtt gtagggggag caggaagtct ttatatagat gaaacaaaaa
     9841 cgacacgact tttagatacg cctgattttc ctgaagaatt taagccacta gcaaaagctc
     9901 aagcggatga attggatctt cttcgtacaa aaaataatct caactggact tttgtcagtc
     9961 ctgctgtaga ttttattccc gatggtgaaa aaacaggaaa ctacattcta gcaggagaaa
    10021 tttttactac taatgaaaaa ggaataagcc agattagtta tgcagattat gctattggat
    10081 tggtagatga acttgaaaaa ggtcatcata taaaagaacg tatctcttta cttgaaaaat
    10141 aataactatg agagtattaa tgtttgacag aaaatttaaa tcttggtaaa atagggattg
    10201 tcgtaaagac aaataacttc ttcttggtta caggcatgcc aacctgtcac tcggatgaag
    10261 ccaaataaaa aggagaaaca tcatggcaat ctcaaaagag aaaaaaaatg aaatcatcgc
    10321 acaatatgca cgtcacgaag gtgatacagg ttcagtagag gttcaagttg ctgtccttac
    10381 ttgggaaatc aaccacctta acgaacacat caaacaacac aaaaaagacc acgctactta
    10441 ccgtggattg atgaaaaaaa tcggtcgccg tcgtaacttg cttgcatact tgcgtaaaaa
    10501 cgacgttaac cgttaccgtg agttgatcaa ctctctagga cttcgtcgct aattcaagat
    10561 acaaaggccg tcaaaagcac aaagcaaaaa taggaaaatt gacgaagaaa cttcagtttc
    10621 taggagattt tatctttttt gccaagtgct taggccgtgt tcaattgagc atatcttgat
    10681 aataaagcta ctctaaattg ggtagctttt ttgcgttgtc accttacctc gatatactca
    10741 agtataatct ttggttacgg ttcctagcac tgtaaggtaa aataaaccag cttttctccc
    10801 ttcggggaga ttttttttgt ttcactgaaa gtttcgtacg atcttcgcct cgtcgcctag
    10861 tctataaacg atttatctag caagaatcat gatgctaagg gcgtcaaaaa tccgtatgaa
    10921 aatagggaaa tgacacagtg ttcgatgagc acaaggagtt tcatcttttt cactaggatt
    10981 ttagcccggg ctcaaatcag ctctctgact tcagagggct tttttattga ggttttatcg
    11041 gtaacaattt tggagactac aaaaacctca aacggtatca gctaattaca ccataaaggt
    11101 gcgcagttac tcggcttttc aagccgagta gctgtatgca agccccctag aagagcccac
    11161 tctttacaaa gtaaagtata gtatgttata ctttacatgg aagtagtcac tgaattccag
    11221 ttagaaatta ctttgtaact acgttttgag gaggagtaaa atgctttcct acgttcgata
    11281 ttacccacta gcgatagcta aattaatgtg tctgtgctct cctaaaatct gctgatttat
    11341 tactgactaa tacaggaggt tttttttatg ggacagacaa tcatatctgc tattggtgtt
    11401 tatatttcca ccagtatcga ttatttaatt attttaatta ttttatttgc acagctatca
    11461 cagaataaac agaaatggca tatttatgcg gggcaatatc taggcacagg cttacttgta
    11521 ggggcgagtt tagttgctgc ttatgtcgtt aatttcgtgc ctgaagaatg gatggttgga
    11581 ttgcttggtt taatccctat ctatttaggg attcgctttg caattgttgg agaagatgcg
    11641 gaagaagaag aggaagaaat tattgaaaga ttagaacaaa gcaaggcaaa tcaactgttt
    11701 tggacagtta cattgctgac aattgcgtct ggcggagata atttaggtat ctatatacct
    11761 tattttgctt cgttagattg gtcacagacc ctcgtggcct tgcttgtgtt tgtaatcggc
    11821 ataattatct tttgcgagat tagtcgggtg ttatcctcta ttccgttaat attcgagaca
    11881 attgaaaaat acgagcgaat cattgtgccc ttagtattca ttctacttgg actatacatc
    11941 atgtatgaaa atggcacgat agagactttt ctgatcgtgt agattttttt gtttcactag
    12001 gattttagcc cgagctcaaa tcagctctct gattttcaga gggctttttc attgagtata
    12061 aatggacaat cttaaagtta ctctattgta gagtggcttt tgatttgatt aggagattct
    12121 tataaatata gtaaaatgaa ataagaatag gacaaatcga tcaggacagt caaatcgatt
    12181 tctaaaaatg ttttagaagt agaggtgtac tattctagtt ccaatctact atataaatga
    12241 aaaccttagc tgtttggcca aggttcattt cttctatgca agttcttctc ttttcttatc
    12301 tgggttccag atgaagcttg cgataaagct aaagatgatg gttaggatag cgaggataat
    12361 agcaaggatg agggcaggga tgcggataaa ccaccagagt ggatttggtt ttttatcatt
    12421 gtgccattgt ttcgtttctt cgtccagacg ttggaattct gtttgaatac gattggcgac
    12481 tgtttcaatg ccttcatcat tcattttctt gatatctgag atatcgattg gatttccaaa
    12541 gttcatatcg acacgttcac ggctaatcaa gcccttcaaa gtcatgggac cggtgtaggt
    12601 aaccggcatg atacggacct tggccatttt ggcaatcagt gctgcgcccc ccttgacatc
    12661 gtttgagtgg cggctaccac ttggaaacat gatgagagag cggtcacttt ttttgagaac
    12721 gttgatagga tatttgatgg ctgaggcgct gggattttca cggtcgatgg gaaaggcgcc
    12781 acacatacga atccaccaac caaagatacg gttggtaaag agttcttttt ttgccataaa
    12841 gatgaactgt tttggcttgg tcgcaaaggc catataaaca ggatcccacc aggtacggtg
    12901 aggcgcaact aaaatataat tttcatcttg attaggaatt ttatcagtat tatgatagtg
    12961 agcattgcca ttgatggacc ataagagcaa tacaactaat ccacgcaaat aagtataaaa
    13021 catgcgatct ccttcgattg ttttcttgtt attattatac cttatcaaag gagggctggc
    13081 aaacttttcc cttgactaga tacatattta ggatgaaatt agaattctgt taaaaaaaat
    13141 gatataatag aatttatgga taaaaataag attatgggat taacccaaag agaagtcaag
    13201 gaaagacagg ctgagggttt ggtcaatgac tttaccgcat cagccagtac cagcacttgg
    13261 caaatcgtta aacgaaatgt ctttaccctt tttaacgctt tgaactttgc cattgctttg
    13321 gctcttgcct ttgtgcaggc ttggagcaat ctggtcttct ttgctgttat ctgctttaac
    13381 gctttttctg ggattgtgac cgagctacga gccaaacaca tggtggacaa gctcaatctc
    13441 atgaccaagg aaaaggtcaa aaccatccgt gatggtcagg aagttgctct taatcctgaa
    13501 gaattagtgc taggagatgt cattcgtttg tctgcaggag agcagattcc tagtgatgcc
    13561 ttggttttgg aaggctttgc ggaagtcaat gaagccatgt taacgggaga aagtgatttg
    13621 gtgcaaaagg aagttgacgg cttacttttg tcaggaagtt tcctagccag tgggtcagtt
    13681 ttatctcaag ttcaccatgt cggtgcagac aactatgctg ccaaactcat gcttgaggct
    13741 aagaccgtta aacccatcaa ctcccgtatc atgaaatcgc tggacaagtt ggctggtttt
    13801 actgggaaga ttatcattcc ctttggtctg gctctcttgc tggaagcctt gcttttaaaa
    13861 ggcctgcctc tcaagtcatc cgttgtaaac tcgtcgacag ctcttttggg aatgttgcct
    13921 aagggaattg cccttttgac cattacttcg ctcttgactg cagtgattaa gttgggcttg
    13981 aaaaaggtct tggtgcagga gatgtactct gttgagacct tggcgcgcgt ggatatgctc
    14041 tgtctggaca agacgggtac catcacccaa ggaaagatgc aggtggaggc tgttcttccg
    14101 ttgacggaaa cgtatggtga agaggctatt gccagcatct tgactagcta catggcccat
    14161 agtgaggata agaatccaac tgcccaagcc attcgccagc gttttgtggg agatgttgct
    14221 tatcctatga tttccaatct tcccttctcg agcgaccgca agtggggggc tatggagtta
    14281 gaaggcttgg ggacagtttt cttaggggca cctgagatgt tgcttgattc tgaagtccca
    14341 gaagctaggg aggccttgga gagaggatca cgtgtcttgg tcttagctct cagtcaggag
    14401 aaattagacc atcacaaacc acagaaacca tctgatattc aggctctagc cttgctggaa
    14461 atcttggacc ccattcgaga gggagcagca gagacgctgg actatctccg ttctcaggag
    14521 gtgggactca agattatctc tggtgacaat ccagttacgg tgtccagcat tgcccagaag
    14581 gctggttttg cggactatca cagctatgta gattgctcaa aaatcaccga tgaggaattg
    14641 atggccatgg cggaggagac agctattttc ggacgtgttt cccctcatca aaagaaactc
    14701 atcatccaaa cgttgaaaaa agcgggacat acaacggcta tgacagggga cggggttaat
    14761 gatatcttgg cccttcgtga ggcggattgt tctatcgtga tggcggaggg ggatccagca
    14821 acccgtcaga ttgccaatct ggttctcttg aactcagact ttaatgatgt tcctgagatt
    14881 ctcttcgagg gtcgtcgcgt ggtcaataac attgcccaca tcgccccgat tttcttgata
    14941 aagaccatct attccttcct gttagcagtc atctgtattg ccagtgcttt actaggtcgg
    15001 tcagagtgga ttttgatttt ccccttcatt ccgatccaga ttaccatgat tgaccagttt
    15061 gtggaaggtt tcccaccatt cgttctgact tttgagcgaa atatcaaacc tgttgagcag
    15121 aatttcctca gaaaatccat gcttcgtgcc ctaccaagcg ctctcatggt cgtcttcagc
    15181 gtcctgtttg tgaaaatgtt tggcgcgagt caaggttggt ctgagttaga aatctcaact
    15241 ctactctatt atctcttggg gtcaattggt ttcttatccg tatttagagc ctgcatgcca
    15301 tttaccctat ggcgtgtcct cttgattgtt tggtcagtag gaggtttcct agccacagct
    15361 ctcttcccaa gaattcaaaa actgcttgaa atttcaacct taacagaaca aacgttgcct
    15421 gtttatggtg tcatgatgtt ggtctttacc gtgattttca tcctgaccag tcgttaccaa
    15481 gcgaaaaaat aaatcaaaac caccagtgtg aactggtggt ttgttctgcg gctataagcc
    15541 gcttctaccg gccagggcca aaggcccacc gaaatagctt cctcgcgcac cactttcccg
    15601 agcaggtgct aaagcacctt agttacttcc tcttatttat ttcgccagta aacggatcta
    15661 ctgactcgaa taacgtgagc tggtctgcta ctctgtcttc ttgtaattga ttctgaatat
    15721 attcagctat cactttctga ttacggccta ccgtatctac ataatagcct ctacaccaaa
    15781 acttgcgatt gccatatttg tattttaaat tcgcatgctt atcaaaaatc atcaaactgc
    15841 tcttgccctt taaatagccc ataaaggacg aaacactaag tttcggagga atactgataa
    15901 gcatgtgaat atggtctgaa caagcattcg cttcatggat tattacaccc ttacgctcac
    15961 ataagtcacg tatgattctt ccgatactag ctttgtatct gccataaatg atttgacgac
    16021 gatatttggg tgcaaaaaca atatgatatt tacaattcca tgtggtatgt gataaacttt
    16081 gattatcctc tctcatgagg tacctcctgt atgatatgtt gtagtggcgg agaaaccact
    16141 tctatcttat cattttagga ggttcttttt gttaccacgc taaaagctct atggaaccac
    16201 tagcatagct agtggttttc gggagacaac aagaaagact gcaatctgtg gattgcagtt
    16261 ttttatacga tggatctatc gtagatctga tgtgcaaggc ctacgtgccg atcatctatc
    16321 ggtgaaccca agagcgaccc tcaagcctgc ttggattgag gtaatagatt caaatatctg
    16381 tagttagact atttgaagtt tgatgtaaga aagagaaagc gacagattga agtaatttta
    16441 actctcttct attgctagaa caaatggtcg gataggttgg tagtttgaaa atgaagatgc
    16501 tatctattgt taaatggaac atagtgttat ttattagaaa atcgtttggt ttatttctta
    16561 tcaaatacga aaagcaactt aaatatttca actaaaatag atgttatgaa gaaaaggtaa
    16621 aatgattttg gcatagtgag gttctgttct atttgatatc atatttttga taaaaacaaa
    16681 aatgtccatt gcaaaggaca aaatgcgaag tatattattt tttgaaagcg atataatgga
    16741 ttcataaagg aggtgtatcg tgtctagaaa acaagaacaa atggaaacgt tgttgctcct
    16801 tttgcgagat agtaaggatt atatatctgc taaagtattg ggagaaaaat taaattgctc
    16861 tgataaaacg gtttatcgcc ttgtcaaggg aatcaacaaa gattgtccgg tagaagcatt
    16921 cattttatct gaaaaaggca gaggtttcaa attaaatcca agaagttccc tcgtggacgt
    16981 tgatgggaat tttacagagg cttttgatcc tgaagtaagg cgtgaaaaat tactagaacg
    17041 tctcttgttg actgctccta agccacattc tatttatgat ttaggagagg aattctacgt
    17101 aagcgagtca gtagtactaa aagatcgtca gatattacaa gagagtctag caatttatgg
    17161 gttagattta aaaatgagac aacgaaagct ttttattgat ggggatgagg ctcaaattcg
    17221 ttcagccatt ctaaatctac tgccaatgtt taatcagttg gatttagagc aaattacaca
    17281 gaataaggtt cagcctcttg acggagaact tgctcacttt tgtttgggat tactgattac
    17341 acttgagaga gaattggggg taaacattcc ctatccatat aatataaata ttttctctca
    17401 cctgtatatt tttatcagta ggaatcgtcg tagtactagt attcatgttg tagcaccttc
    17461 aaaacctact attgttgatg agaaaattta cagtgtctgt caaaaaatta ttcaagaaat
    17521 tgaacaatat tttaggatga aggttgatgc agttgagatt gactatcttt atcaatacgt
    17581 tgtatcttcg agattgcaaa aaccattttc ttccgggaag cttccttttt ctcagcgagt
    17641 tttagatgtc actcattact attttagccg tatgtgtatg gacaatagag agattgaaac
    17701 gacagatcct gactttgttg acttggcgag tcatatcagt cccttactga ggagattaga
    17761 taatagagta cagattaaga atagtctttt atcacaaatt cttttaacct atcctaatct
    17821 ggttaaagag ttaacaacta tttctaaaga agtgagtcta gtatttggtt ttgcttcctt
    17881 gagtctggac gagattggtt ttctagtctt atattttgca cggtttcaag aaaagcgagc
    17941 acgtcctcta aaaacagtag tgatgtgtac atcaggtgtc ggaacttcag agcttttacg
    18001 agcacgatta gaaaagcaat tttctgaatt ggatattatt gatgtagttg cttatcatca
    18061 attagatgag ctgataaatc tatatccaga tttagatttc attgtgacga cggtagcttt
    18121 gcaggaacca gcaagtgtcc cgtttgtcct agttagtgtt tttctaaccg agggtgataa
    18181 acaacgtctt caagcaaaaa ttcaggagat aaactatgaa taatctttcg cttgtcctta
    18241 tggatatatc tgttcaaaat cgtcaagaag cctacaaaga attagcaaat caaatcagcc
    18301 ttcttgtttc tgaagataca gaaaaaatag aagagcttct atattaccgt gagagacagg
    18361 gaagtataga ggttgctaaa ggtgttcttc taccacattg tgaaggaaac tttcaacatc
    18421 atgtcttagt gattactaga ttaaaatcac ctatcagaga atggtcgaag gatatccagt
    18481 gtgttgacct tattatcggt ttggccattg cagtatcaca ggacaagtca tgtattaaaa
    18541 cattgatgag aagactagca gatgaatcat tcataaatca attaaaacag ttaacaaaag
    18601 aagaattacg ggagataata tatggaaatc aaagatattc ttaatgtgag tctgatccag
    18661 acggatttac agatgcagag caaagaagag gtttttgagg cattagctca actattggtt
    18721 gagacgggtt atgtgtctga tagagaccaa tttatcgaag gtctttatca gagagaggca
    18781 gaaggacaga ccggtattgg gaattatatt gctattcccc atagcaagag ttctgctgtg
    18841 gagaaggcgg gggtagtcat agctataaat cacaatgaga ttccttggga gaccattgat
    18901 gggaaagggg tcaaagtaat tgtactcttt gcagttggtg atgatacaga agctgctagg
    18961 gagcatttga agaccttatc actctttgct cgaaaacttg gtaatgacga agttgttgcc
    19021 aaattagttc gggctcagac atctgatgat gtgattgcag ctttttgtta ataagaaaaa
    19081 attttggagg gtatccgtat gaaaattgtt ggtgttgcag cttgtactgt gggaattgcc
    19141 cacacttata ttgcacagga aaaattagag aatgccgcaa aggtagctgg acatgtgatt
    19201 catgttgaga ctcaggggac aataggggta gaaaatgaat tgagtcaaga gcagattgat
    19261 gcagcggatg tagttatttt agcagttgat gttaagattt ctggtatgga acgctttgag
    19321 ggtaaaaaga ttatcaaggt tccaacagaa gtggcagtca aatctcccaa taaactgatt
    19381 gctaaagctg ttgagattgt tacgaaataa ctgaaaatat ttaaggagaa aatatatgtt
    19441 gaaacactta aacttaaaag gtcacttatt gacagccatt tcctatatga ttccaattgt
    19501 ttgtggtgca ggattcttag ttgccattgg tttagcaatg gggggtggtg ttcctgacgc
    19561 tcttgtagca ggaaaattca ctatctggga tgctttagca actatgggtg gtaaagccct
    19621 tggtctcttg ccagttgtta ttgctacagg tttgtcttac tcgattgctg gtaagccagg
    19681 gattgcacca ggttttgttg ttggtctaat tgccaattct gttggttcag ggtttatcgg
    19741 tggtatcttg ggaggttata tagctggttt cttggttcaa gcgattatta aaaaggtcaa
    19801 agtaccaaac tggattaaag gtttaatgcc aaccttgatt attccttttg tagcctcttt
    19861 ggtaagtagt ttgattatga tttatattat tggagcgcct atcgcagcct ttaccaactg
    19921 gttgacgagc ttattacaaa gcttgggaag tgcttcaaat ggtttgatgg gggcagttat
    19981 tggaattctc agtgctgttg actttggtgg cccacttaat aaaacagtct atgcgtttgt
    20041 gttgacttta caggctgaag gtgtgaaaga accattgact gctttacaat tggtgaatac
    20101 tgctacacca gttggatttg gattggccta ttttatcgcg aaattactca aaaaaaatat
    20161 ctatactcaa gaggaaatcg aaacattgaa atcggctgtt cctatgggga ttgtcaatat
    20221 tgttgaaggt gtaattccga ttgttatgaa taacttggtt ccaggtctca ttgcaacagg
    20281 tatcggtggt gctgttggtg gtgctgtttc tttgacaatg ggtgctgatt ctgctgtgcc
    20341 atttggtgga gtgcttatgt taccaaccat gactcgtcca gtagctggta tttgtgcctt
    20401 gttagctaac attgtagtca caggacttgt ctacgcgatt ttgaaaaaac caataaaaca
    20461 tgcagaacca gttatgactg ttgaagaaga gattgatttg tcagatattg aaattttgta
    20521 agagggtaac gatgtcaaga attgaatttt caccatcttt gatgaccatg gatttggaca
    20581 aattcaaaga gcagattact tttttgaatg ataaagtagc atcttatcat atcgatatta
    20641 tggatggcca ttttgttccc aatattacct tgtctccttg gttcattcaa gaagttcaaa
    20701 aaattagtga cacaccttta tcagttcatc tgatggtcac agacccaacc ttttgggtag
    20761 atcaagttct cgatttacaa tgtgagtata tttgtattca tgctgaagtt ctgaatggtc
    20821 ttgcttttcg tttgattgat aaaattcatg atgcaggtct aaaggctggt gttgtcctta
    20881 atcctgaaac acctgtttct acaatctttc cctacattga tttacttgac aaagcaacta
    20941 ttatgactgt agatccaggt tttgcaggac aacgcttttt ggagtctacc ttgtataaaa
    21001 tccaagaact ccgtcagctt agagttcaga atggttatca ctacatcatt gagatggatg
    21061 gttcttcgag tcgtaagact ttcaaacaaa ttgatgtggc aggaccagat atttatgtta
    21121 taggtcgcag tggattattt ggtttggatg acgatattgc caaagcctgg gatatctgtt
    21181 ctagagatta cgaagaaatg accggaaaaa caatgccaat caaataatgg tttgagaaga
    21241 aatttattag ttaggaggaa tatatgtcac tacaatcagt taacgccatt cgttttcttg
    21301 gcgtagatgc tattaacaaa tctaattctg gtcacccggg aattgtcatg ggtgctgcgc
    21361 caatggctta tagcctattt acaaagcacc ttagaattac acctgagcag ccaaactgga
    21421 ttaaccgaga tcgctttatc ttgtctgcgg gtcatggatc aatgctactg tatgctctct
    21481 tgcatttaac agggtataag gatgtatcca tggacgagat taaaaatttc cggcaatggg
    21541 gatctaagac acctggtcat cctgaagtga cgcatacgtc tggtgtggat gcgacatctg
    21601 gtccgcttgg tcaggggatt tctactgccg ttggtttcgc ccaagcagag cgttttttag
    21661 ctgctaagta caacaaagat ggtttcccta tttttgacca ttatacttat gttatcgctg
    21721 gagacggtga cttcatggaa ggagtgtctg cggaggcggc ttcttatgca ggtcatcaag
    21781 ctttagataa gcttatcgtc ctctacgact ccaacgacat ctgcttggat ggtgagacca
    21841 aagatacttt ctctgaaaat gttcgcgtcc gttacgatgc ttatggttgg catacagttc
    21901 tggtagaaga tggaacagat ttagcagcaa tttctacagc aattgagacg gccaagtttt
    21961 ctggtaaacc gagtttgatt gaagtgaaaa cggtaattgg ttacggctca cccaataaaa
    22021 gtggtacaaa tgctgttcat ggtgcaccac taggagcaga agaaacagga gcaactcgta
    22081 agtttttggg atgggattac gatccatttg aagtaccaga ggaagtatat tctgatttca
    22141 agacaaatgt agcggatcgt ggtcaggagg catacgatgc ttgggctagt ttggtgtctg
    22201 attacaaggt tgcttatccc gaagttgcta gtgagattga cgctattgta gctggaaaat
    22261 cccctgtaac cattactgaa aaagacttcc ctgtctatga gaatggcttc tctcaagcaa
    22321 ctcgtaattc gtcccaagat gctattaata cagcagcagt tttaccaacc ttcttaggtg
    22381 gatcggcaga cttagctcac tctaacatga cctacatcaa ggcagatggc ttacaagata
    22441 aatataatcc attaaaccgc aatattcagt ttggggtacg tgaatttgcc atgggaacaa
    22501 tcctcaatgg aatggctctt catggtggtt tacgagttta tggcggaacc ttctttgttt
    22561 tctctgacta cgtcaaagct gctattcggc tatcagccat tcaggagttg cctgtaactt
    22621 atgtctttac ccatgattca attgccgttg gtgaagatgg tccaactcat gaaccagttg
    22681 aacatttggc aggtttacgc tcaatgccaa acttgactgt tatccgtcca gcggatgccc
    22741 gtgaaactca agcggcttgg catcatgcct tgaccagtac caccactcca actgtcattg
    22801 tcttaacccg tcaaaacttg gtagttgaag aagggacaga ctttggtaag gtcgctaaag
    22861 gagcctacgt cgtgtatgat accccgggat ttgatactat tatcattgct acaggatctg
    22921 aggtcaatct agctatcaaa gctgctaagg aattggtttt acaaggtggt aaagtacgtg
    22981 tggtatctat gccctcaacc gaactatttg atgctcaaga tgctacctac aaggaagaca
    23041 ttttaccatc taagactcgt cgtcgtgtgg ccattgaaat ggcagcgacc caaagttggt
    23101 acaagtatgt tggtttggat ggcgcggtca tcggtattga catcttcggt gcgtctgccc
    23161 cagctcagac tgtgattgat aattatggat ttacggtaga gaatatcgtt gctcaagtta
    23221 agtccctata gaaaccaatt acaatgaaga tacagctgtt gtcagactag cagatgtagt
    23281 gatagacact aatcagatga ttggttattt aaaaactgta atgaaaatgt aataatttat
    23341 ctacgaaagt tatagtagat agtatacaca atagagtata ccctgaaacg gttgcgaagt
    23401 acgctaatca ctttgctact gatctagata gtttctttaa tcaataaaca cagcatccac
    23461 agattgactt aggatattgt aagttttttg aaagctagag agaaggtctc taaaattaaa
    23521 aaacgcatag tataggatgt tgaaatgatg aactgcaccc caaaagttag acagaaaaaa
    23581 atctaacttt tggggtgttt ttattatgaa attaacttat gatgataaag ttcagttcta
    23641 tgaacttaga aaacaaggat atatcttaga gaagctttca aataaatttg ggataaataa
    23701 ttctaatctt aggtacatga ttaaattgat tgatcgttac ggaatagagt tcgtcaaaaa
    23761 agggaaaaat cgttactatt ctcctgattt aaaacaagaa atgattcata aagtctgaca
    23821 tgaaggctgg actaaagata gagtttctct tgaatacggt ctcccaagtc gtacgatact
    23881 tcttaactgg ctagcacaat acaggaaaaa cgggtatact attgttgaga aaacaaaagg
    23941 gagagtacct gagagcggag aatgccatcc taaaaaagtt aagagaactc cgattgaagg
    24001 aggaaaaaga gaaataagaa agacagaaat tgttcaagaa ttaatgactg agttttcgtt
    24061 agatcttctt ctaaaagcca ttaaactagc tcgttggacc tactactatc acttgaaaca
    24121 gctagataaa ccagataagg accaagagct taaagctgaa attcaatcca tctttatcga
    24181 acacaaggga aattatgctt atcgccgggt tcatttagaa ctaagaaatc gtgcttatct
    24241 ggtaaatcat aaaagagttc aaggcttgat gaaagtactc aatttacaag ctaaaatgcg
    24301 acagaaacga aaatattctt ctcataaagg agacgttggt aagaaagcag agaatctcat
    24361 tcaaggccaa tttgaaggct ctaaaacaat ggaaaagtgc tacacagatg tgacagaatt
    24421 tgccattcca gcaagtactc aaaaacttta cttatcacca gttttagatg gctttaacag
    24481 cgaaattatt gcttttaatc tttcttgttc gcctaattta gaataagtac aaacaatgtt
    24541 ggaacaggca ttcaaagaga agcactatga gaatacgatt ctccatagtg accaaggctg
    24601 gcaataccaa cacgattctt atcatcggtt cctagagagt aagggaattc aagcatccat
    24661 gtcacgcaag ggcaacagcc cagacaacgg catgatggaa tctttctttg gcattttgaa
    24721 atcggagatg ttttatggtt atgagaagaa ctttagatct ttagaaaacc ttgaacaagc
    24781 tattgtggac tacattgatt attacaacaa caagagaatt aaggtaaagc taaaaggact
    24841 tagccctgtg caatacagaa ctaaatcctt cggataaatt aattgtctaa cttttggggt
    24901 gcagtacatt tttggtatat ataaaatttg taggagctat atctacaatt ttatattccc
    24961 agtttatgga tgtaacttac tatattcaca atgttatcca gtgttttttc tctaatattt
    25021 aaggagtgtt ctgtttctcg aataaattct tcaaagttta acccgtcaac ttgttcctga
    25081 acaagaaaat aatcatccac gatataaaat tcatcagtta aattagtagt ataactttta
    25141 tcggctaatt tttttagcat gtgagcttca ttttttatat catcaagagc tgtccattct
    25201 ccttcagcat cataattcac aaaaggtctt gactgcttga tgattacttt ttgcccgtcc
    25261 gattttctaa ttgcccgata aacatttcct ttatttgatc tcttaataat tttttccatt
    25321 ttgtatttat ttattgcaga gtccttactt gaaacttcac atgtggtttg aaaataaatc
    25381 cttttttctt cttctgaaaa taaatccatt ttccagctag gaagactagg gaaattttgt
    25441 ctcttatctt ctacatagtt cttccttttt tcatctagca ataaatagat gactttttta
    25501 tttttttcat cataagcttg tttttttaaa aaagccccat atctataatg aactggagaa
    25561 tgcattccac attgatagtc agatagtatt tttggagcct taaattctga cagtctattc
    25621 gtaagattac aaatcatact cttagcttca gattctgact taggatatag agttataaat
    25681 ttgttagcag tagggctcat ttccctaggg gaattaattt tttttaattc ctctaaattt
    25741 ttaacaactt taaagctaca attatttagt tgggatagtt tatacacaat cttaaaaata
    25801 tttacagcgt cttttatttg ggagcttatg tgtattttcc acccttgtgc aggcatctta
    25861 tctgataaat aatagtaatt ccaaatatca ccctgttgga aatttttatc acattttaaa
    25921 cattctttct ctaataaatc cataacaaac tcctaattat taaaataggc tagagacaca
    25981 gcactagcct attccgtaag taaagctact tattttatat aaaatataac tagcgacctg
    26041 taattaaaca agtaaaagaa caatacttag ggagacctgt gacaatgata tcttcctctg
    26101 gaagtagttc taattcaaac aatgtttgtt ccatgactgt atcctcctgt tatgataaat
    26161 tccagaaagc atagagcaaa ttcgtggttc gtctgactaa atttcttatc tgaacaccac
    26221 cttcaaatac aaattttagt caaactttta ttttttctaa ctttctaatt atattctacc
    26281 atgtgttttt atatgtcaag aattttctga ataaactgtt tattttttat tttttgacat
    26341 ttttgcaaat tgtttaaaat ctttcaattg gattcactta ttactttagg acattgaaac
    26401 ttctttcgcc agaggtgctt atattactgg gaagttgaag attgaaatgt ggtataataa
    26461 gaggtaatag agttttggaa agtgagagaa gatgatttca aagagattag aattggtagc
    26521 ttcctttgtg tcacaggggg ctattttact agatgtggga agtgaccatg cttatctgcc
    26581 tatcgagttg gttgagagag gccaaatcaa aagcgctatt gcaggtgagg tggtggaagg
    26641 tccctatcag tctgcggtta aaaatgttga ggctcacggc ctaaaggaga aaatccaagt
    26701 ccgtttagcc aatggcttgg cagcttttga agagactgac caagtgtctg tcattaccat
    26761 tgctggcatg ggtggtcgtt tgattgctag gattttagaa gaaggtttgg ggaagttagc
    26821 taatgtagag cgtttgatcc tccagcccaa taatcgtgaa gacgacttgc gtatctggct
    26881 acaggatcat ggattccaga ttgtagcaga aagcatctta gaagaagctg gaaagtttta
    26941 tgagattttg gtggtggaag caggacaaat gaagctatca gccagtgatg ttcgctttgg
    27001 tcccttcttg tccaaagaag tcagtccagt atttgtccaa aaatggcaaa aagaagctga
    27061 gaagctagag ttcgccctcg gacaaatccc agaaaaaaat ctggaagaac gtcaagttct
    27121 agtagataag attcaagcta tcaaggaggt gctccatgtt agcaagtgaa gtgatccaag
    27181 cttatgaagc cttttgccct caggaatttt ctatggaggg agacagccgt ggtctgcaaa
    27241 ttggcacttt agacaagggt atccaaaggg tcatggttgc cctcgatatt cgtgaagaga
    27301 cagtggcaga ggccattgaa aagggtgtgg acttgattat cgtcaagcac gcgcctatct
    27361 ttcgtcctat caaggacttg cttgccagcc gtccgcaaaa tcagatttac atcgacctga
    27421 ttaagcatga tatcgcagtt tatgtcagcc ataccaatat tgatatcgtt gaaaatgggc
    27481 tcaatgactg gttttgtcag atgctaggaa tcgaggagac gacttatctg caggaaacag
    27541 gtccagaacg tggaattgga cgtattggga atattcagcc tcagacattt tgggaattgg
    27601 cccaacaggt caagcaagtc tttgacctag atagccttcg aatggtgcat tatcaagagg
    27661 atgatttgca gaagcctatt tcaagagtag caatttgtgg tggaagcggg cagtctttct
    27721 ataaggatgc tttggcaaag ggggcagatg tctatatcac tggtgatatc tactaccata
    27781 ctgctcagga tatgctatct gatgggttgc ttgcattgga cccaggtcac tatatcgaag
    27841 tgatttttgt agaaaaaatc gcagcacttc ttagtcaatg gaaggaagat aaggggtggt
    27901 ctattgatat cttgcctagt caggcatcaa ccaatccttt ccatcatatc tagttagaaa
    27961 gtgaagacaa tgaaaaaagt tgccattatt ggagcaggaa ttgtgggagc aacagctgcc
    28021 tactacctct cgagagaaag tgacctagag gtgaccgttt ttgaccatga gcaaggtcaa
    28081 gccaccaagg ccgcagcagg aattatcagt ccttggtttt ccaaacgccg taataaagcc
    28141 tggtacaaga tggcgcgctt gggggctgat ttttatgtgg atttattagc tgatttagag
    28201 aaatcaggac aagaaatcga cttttaccag cgttcgggag tctttctctt gaaaaaggat
    28261 gaatccaatt tggaagaact ttatcaactg gccctccagc gcagagaaga atctcccttg
    28321 atagggcaat tagccattct gaaccaagcc tcagctaatg aattattccc tggtttgcag
    28381 ggatttgacc gcctgctcta tgcttctggt ggagcgagag tagatggcca acttttagtg
    28441 actcgtttgc tggaagtcag tcatgtcaag ctggtcaaag aaaaagtgac tctgacaccg
    28501 ttagcatcag gctaccagat tggtgaagag gagtttgagc aggttatttt ggcgacggga
    28561 gcttggttgg gggacatgtt agagccttta ggttatgaag tggatgtccg tcctcaaaaa
    28621 ggacaactac gagattatca gcttgcccaa gacatggaag attaccctgt tgtcatgcca
    28681 gaaggggagt gggatttgat tccctttgca ggtgggaaat tatccttagg cgctacccac
    28741 gaaaatgaca tgggatttga tttgacggta gatgaaacct tgctccaaca aatggaggag
    28801 gccaccttga ctcactatct gattttggct gaagctactt caaaatctga gcgtgttgga
    28861 atccgtgcct acaccagtga tttctctcct ttctttgggc aggtgcctga cttaactggt
    28921 gtctatgcag ccagtggact aggttcatca ggcctcacaa ctggtcctat cattggttac
    28981 catctagccc aactgatcca agacaaggag ttgaccttgg accctctaaa ttacccaatt
    29041 gaaaactatg tcaaacgagt aaaaagcgaa taatactcaa taaaaatcaa agaacaaact
    29101 aggaaactag ccgcgggtta ctcaaagcac tgctttgagg ttgtggatag aactgacgaa
    29161 gtcagctcaa aacactgttt tgaggttgtg gatagaactg acgaagtcag taaccatacc
    29221 catggcaagg cgacgttgac gtagtttgaa ttggattttc gaagagtata agcattttac
    29281 tgaaatttta tcaggtagtt taggatggca aatgacattc cctgtcaaaa atggtaaaat
    29341 aagagaaaat aatctgagaa tcgaggaaaa aagatgcaag aaaagatttt ggtaactggt
    29401 ggtgccggtt ttattggaac ccacactgtt attgaattga tccaagcagg tcatcaagtt
    29461 gttgtggtgg ataatcttgt caatagcaat cgtaagagtt tagaagttgt tgaaggaatc
    29521 acaggagttg agattccttt ctatgaggca gatattcgtg acacagatac cctcagagat
    29581 attttcaagc aggaagaacc gactggtgtc attcactttg ctggtttgaa ggctgttggc
    29641 gaatcaacac gtatccctct tgcctactat gacaacaata tcgctggaac tgtcagcctt
    29701 ttgaaagcca tggaagaaaa caactgtaaa aacatcatct tcagttcttc tgcgacagtt
    29761 tacggggatc cgcacacagt gcccatcttg gaagatttcc cactttcagt gaccaaccca
    29821 tacggtcgta ctaagctcat gctagaggaa attttgactg atatttacaa agcagactca
    29881 gaatggaatg ttgtcttgct tcgttacttt aacccaatcg gagcccatga gagtggtgat
    29941 ttgggagaaa atccaaacgg tattccaaac aatctcttgc catatgtgac tcaagtagcc
    30001 gttggaaaat tagagcaagt gcaagtgttt ggagacgatt acgatacgga agatggaaca
    30061 ggtgttcgtg actatatcca cgttgtcgat ttggctaagg gtcacgttgc agctttgaaa
    30121 aaaatccaaa aaggttcagg actaaacgtt tataaccttg gaactggtaa aggttactca
    30181 gttcttgaaa ttatccaaaa catggaaaaa gcggtgggcc gtcctattcc ttaccgcatc
    30241 gtagaacgtc gcccaggtga tatcgctgcc tgctactcag acccggcaaa agctaaagca
    30301 gaactcggtt gggaagcaga actcgacatc acccaaatgt gtgaagacgc atggcgttgg
    30361 caaagcaagc atccaaatgg atttgaagac taagatgatg atttcaatca tcgtcccttg
    30421 tttaaacgaa gaggaagtac ttcctctttt ttatcaggct ctggaagctt tacttccaga
    30481 tttggaaaca gaaatcgagt atgtctttgt cgatgatgga tcaagtgatg ggaccttgga
    30541 actcttaaag gcctatcggg agcaaaatcc ggcagtccat tatatttctt tctctcgaaa
    30601 ttttggcaaa gaagcagccc tttatgcagg cttgcaatat gcgacaggag atttggtggt
    30661 ggtgatggat gcagacctcc aagatcctcc tagtatgttg tttgagatga aaaatgtatt
    30721 agacaaaaat gtagacttgg actgcgttgg gacacggaga actagtcggg agggagaacc
    30781 cttctttcgc agtttctgtg ctgttctctt ttatcgcctc atgcaaaaaa tcagcccagt
    30841 agctctgccg tcgggtgtcc gtgattttcg tatgatgaga aggtctgtag tcgatgccat
    30901 tttaagcttg accgagtcca atcgtttttc aaaggggctc ttcgcctggg tgggtttcaa
    30961 aacctactat ttggactatc caaatgtcga aaggcaggct ggcaagacaa gttggagttt
    31021 taggcaactc tttttctact ccattgaagg gattattaat ttttcagatt ttcccttgat
    31081 tatagccttt gtagctggtc tcctatcttg ttttctttct ctgctgatga ccttttttgt
    31141 tgtggttcgg accctcattt tgggcaatcc gacatctggt tggacctctc tgatggctgt
    31201 tattctcttt cttggaggca ttcaactctt gaccattggg attctcggca agtatatcag
    31261 taagatttat ttagagacta aaaaaagacc actttatctt atcaaagaaa aaagtgacct
    31321 tcctgatttt acagaaaaaa ataaagtgaa aagactataa ttttacatgg aaatgtgcta
    31381 aactagaggg agtggattat cgccattgat ttaggcttcc tggtttccaa tcagggcgcg
    31441 agttgggcaa tttttaacag cctctaagat atcctgacta ggagaaattt ctttttccag
    31501 ttggtcaggg tcatcgtaaa aacgcacgat tccattatcg tggtaatcaa ataaatcaga
    31561 ataagtttgg caaagcccac aggcgataca tcgttcaggt ataagtgtga ttttcatgtt
    31621 catatttata ttgtaataag aaaagtaaaa aaaaacaagg agtaaggtat ggcaaaagaa
    31681 ccgtggcaag aagatatcta tgatcaagaa gaatcaagag cagagcgtcg gcatcgaaac
    31741 cacggagggg ctgataggat ggctaatcgt attttgacga tcctagctag tattttcttt
    31801 gtaattgtgg tggtgatggt catcgttctc atctatctat catcgggggg gagtaatcgc
    31861 acagcagcct taaaaggctt tcatgattct gatgccagtg tagtacaaat ctcatcttca
    31921 agtagttctc agcctgagca gagttcagag ccagaatcta cttctagtag ttcagaagaa
    31981 gctgctaatc ctgaaggaac gattaaagtt ctcgcaggag aaggggaagc agctattgcc
    32041 gctcgtgcag gaatctccat tgctcagtta gaggccttga atcctgggca catggctaca
    32101 ggatcttggt ttgctaatcc aggtgatgtt ataaaaataa aataggagcc gatatgaaaa
    32161 caattcaaat tgctattgat ggtcctgctt ccagcggtaa gagtacggtc gcaaagatta
    32221 ttgctaagga ttttggattc acctaccttg atacaggagc tatgtatcgt gcagcgacct
    32281 atatggctct taagaaccaa ttaggagttg aagaagtcga agcccttcta gccttgttgg
    32341 accagcatcc aatcagcttt ggacgttcag aaactggaga ccagcttgtt tttgtaggag
    32401 atgtggatat tacccatcct atccgtgaaa atgaagtgac caatcatgtt tctgctattg
    32461 cagcaattcc tcaagtgcgt gagaaactgg tttctctcca acaagaaatt gcccagcaag
    32521 gcgggattgt catggatggt cgcgatattg gaactgttgt attgccacaa gcagaattga
    32581 aaattttcct agtagcttct gttgatgaga gagcagagcg tcgttacaag gaaaatattg
    32641 ccaagggaat tgaaacagac cttgaaaccc taaaaaagga aattgctgcg cgtgactaca
    32701 aggatagtca tcgtgagact tctcctctca aacaagcaga ggatgctgtc taccttgata
    32761 caactggttt gaacattcaa gaagtagttg aaaaaatcaa agcagaagct gaaaaaagaa
    32821 tgtaaatgta gaaaagccga tagattgata tcggtttttt tctaaatttg tcaaaatgct
    32881 gattttaggg tataatagtt gctgttcgag aaattttgat tttcaaagaa tagtgaatga
    32941 tgataaggag aaaccatgaa caatttacca aattgtccaa aatgtaactc agagtatgtc
    33001 tacgaagacg gtgccctact ggtttgccca gagtgtgctc atgagtggaa tcctgctgaa
    33061 gttgcagaag tagaagaagg tcttgtcgct atcgatgcca acggaaataa attggctgat
    33121 ggtgatacag taactctcat caaggacttg aaagtaaaag gtgcgccaaa agatttgaaa
    33181 caagggacgc gcgtgaaaaa tatccgcatc gtagaaggcg accacaatat cgactgtaag
    33241 attgatggtt ttggtgccat gaaacttaaa tcagagtttg tgaggaagat ttaagcatgt
    33301 caaaacacta taaacttgta ttttatagcc gtatcttctt gtttctagcg gcttttacgg
    33361 gagtttatct tgaaattact aagcatggtg gttttgggat gcttctctat tacacggttc
    33421 tgtccaacct cttggtaact atttttaccc tttatcttct aaaggttatg agccgtgtag
    33481 gtgaaaattg gcaaaggcca agtctcttgc gcttaaaagg tggggtcacc atgagtatca
    33541 tgattacctg tgtgatttac catttcctct tggcgcccat tgcgactaat ttctataccc
    33601 tagaaaattt cctttgccac tatatcgttc ccatctggtt tttagcggat accctctttt
    33661 ttgacaaaca gggtcaatac aagatttggg atccagcagt gtggaccatt ttaccctttc
    33721 tgtatatgat gtttgctctt tttaatggct tggttctaaa actcaatatt ccaaatgcca
    33781 aggataatcc tttcccttac ttctttttga atgtgaacaa gggttggaat gttgtgttta
    33841 agtggtgtct gattatcttt gttgcctata tggtagcagg atttattttc tactttatca
    33901 agcaaatcaa gagaaagtca tcctaatact cttcgaaaat ctcttcaaac cacgtcagtt
    33961 tccatctgca acctcaaaac actgttttga acaacctgcg gctagcttcc tagtttgctc
    34021 tttgattttc attgagtata agatacaagg gattcatttt cgaatttaag aaggagtcta
    34081 caaaccaatg aggcttcttc ttttcttgct tatttgataa ctatcaaaaa agtagaagat
    34141 taagagaaat atagaaagag atttcatgaa acaattttta gaacgggcca gcattttggc
    34201 tctctccctc gttttgatta cctccttttc catttcgagt gccctaccag ccatgtttga
    34261 ctattatcag ggttattcta aggaacaaat tgagctcttg gtgagcttgc cttcctttgg
    34321 aatcatgatg atgttactgc taaatggttt cttagaaaaa atatttcctg agcgcttaca
    34381 gattagtttg ggcttgctga ttttatcatt gagcggtaca gctcccttct ggtaccaagc
    34441 ctatcccttt gtctttggaa cacggcttct ctttggtttg ggtcttggga tgatcaatgc
    34501 caaggccatt tctattatca gtgaacgcta ccaaggaaaa aggcgaattc agatgttagg
    34561 gctacgcgct tctgcagagg tcgttggagc ttctctcatt accttggccg tcggtcagtt
    34621 gttggccttt ggttggacag ctatctttct agcctatagt gctggatttt tggtgctgcc
    34681 cctttatctg ctctttgtcc cttatggaaa atcaaagaaa gaagtcaaga aaagagcgaa
    34741 ggaagcaagt cgtttaactc gagaaatgaa aggcttgatt tttaccttag ctatcgaagc
    34801 ggcagttgta gtttgtacca atacagctat taccatccgt attccaagtt tgatggtgga
    34861 aagaggattg ggggatgccc agttatctag ttttgttctt agtatcatgc agttgatcgg
    34921 gattgtggct ggggtgagtt tttctttctt gatttctatc tttaaagaga aactgctcct
    34981 ctggtctggt attacctttg gcttggggca aatcgtgatt gccttgtctt catccttgtg
    35041 ggtggtagta gcaggaagtg ttctggctgg atttgcctat agtgtagtct tgacgacggt
    35101 ctttcaactt gtctctgaac gaattccagc taaactcctc aatcaagcaa cttcatttgc
    35161 tgtattaggc tgtagtttcg gagcctttac gaccccattc gttctaggtg caattggctt
    35221 actaactcac aatgggatgt tggtctttag tatcttagga ggttggttga ttgtaatctc
    35281 tatctttgtc atgtacctac ttcagaagag agcttaggat tgattcctaa gtttttcttt
    35341 tgatactcaa tgaaaatcaa agagcaaact atagttgatt gagtttggaa tagtacgctg
    35401 tagctgctaa aatatttcta gaaattaatt tgactttcct aatagagttg ttcatatctt
    35461 atttcaatct actatagaaa gctagccgca ggttgctcaa aatagtgtgt tgaggttgca
    35521 gatggaaact gacgtggttt gaagagattt tcgaagagta tgagattttt atacccatac
    35581 aatatttgtt ttaggtcctt gtttacagac taatttcttg ataaaataga ggctatgacg
    35641 agatataaag caactatttc ctatgatggt tatgcctttg ctggctttca gcgccagcct
    35701 catgcgcgta gcgttcagga agaaattgaa aaaaccttga ccagattaaa taaagggcaa
    35761 gccattactg ttcacggtgc tggtaggaca gatagtgggg ttcatgccct gggacaggtg
    35821 attcattttg acctgcctta tcagatggat gaggagaaac tccgttttgc cttggatacc
    35881 cagtctcctg aagatattga tgtgatttcg attgagcttg tggcagatga ttttcattgc
    35941 cgttatgcca agcatagcaa gacctatgag tttactgtgg atagaggacg tcccaaaaat
    36001 ccgatgcgcc gtcactatgc cacccacttt ccctacccac tcgatgtgga acgaatgcag
    36061 attgcaatca aaaagctaga gggaacccat gattttaccg gttttacagc ctctgggact
    36121 agtgtagagg ataaggttcg caccatcaca gaagctagtt taatagtcga tgagacagga
    36181 caatttttga cctttacctt ttcaggaaat ggtttcttgt ataaacagat tcgcaatatg
    36241 gtggggactc ttctcaaaat cggtaacaac cgcatgccag tagagcagat tgatctcatc
    36301 ttggagaaga aggacaggca acttgcaggt cccactgcag caccaaatgg tttgtattta
    36361 aaggagattc gttatgaaga ataatcgtat tttagcactt tctggaaatg atatttttag
    36421 tggtggtgga ctgtcagctg atttggctac ctataccttg aacggcttgc atgggtttgt
    36481 agcagtgact tgtttgacag ccttgacaga aaaaggattt gaagtctttc caactgatga
    36541 taccattttt caacatgaat tagatagctt gcgtgatgtg gaatttgggg gaattaagat
    36601 tggtcttctc cctactgtca gcgtggctga gaaggccttg gactttatca aacaacgccc
    36661 aggagtacct gtggtgttgg atcctgtctt ggtctgcaag gaaacgcatg atgtagctgt
    36721 cagtgagctc tgccaagagt tgattcgctt tttcccttat gtcagtgtga ttacgcctaa
    36781 tctcccagaa gcagaattat tatccggtca ggaaattaaa accttggaag acatgaaaac
    36841 tgcagcgcag aaattgcatg atttaggagc gccagcagtc attatcaagg gaggcaatcg
    36901 tcttagtcag gacaaggctg tggatgtctt ttatgatgga cagaccttta ctatcctaga
    36961 aaatccagtt atccaaggcc aaaatgctgg tgcaggttgt acctttgcct ctagcattgc
    37021 cagtcacctg gttaaaggtg ataaactttt gccagcagta gaaagctcta aggctttcgt
    37081 ttatcgtgct attgcacaag cagatcagta tggagtaaga caatatgaag caaacaaaaa
    37141 caactaaaat cgcccttgta tccctattaa ccgccctttc tgtggttcta ggttatttct
    37201 taaaaatccc aacacctaca ggaattctaa ctcttttaga tgctggtgtc ttctttgcgg
    37261 ccttttactt tggtagtcgt gaaggagcgg tagtcggagg actagcaagt ttcttgattg
    37321 acctcttatc aggctaccct cagtggatgt tctttagctt ggtcaaccat ggcttgcagg
    37381 gatttttcgc aggatttaaa ggaaaaagtc agtggttagg ccttatttta gcaactattg
    37441 ccatggtagg aggctacgcc ttgggttctg ctttgatgaa tggctgggca gcagccctcc
    37501 cagaaattct accgaatttt atgcaaaata tggtagggat gattgtagga tttattctta
    37561 gtcaaagtat caagaagatt aagtaatact cttcgaaaat ctcttcaaac cacgtcagct
    37621 tccatctgca acctcaaaac actgttttga gcagcctgag gctagctttc tagtttgcac
    37681 tttgattttc attgagtata aagaggttga gtaaaaagta tttcaaataa gaaaaacgca
    37741 tagtatcagg tgttgaatga actgcccccc aaaagttaga cagaaaaaat ctaacttttg
    37801 gggtgttttt attatgaaac taacttatga tgataaagtt cagatctatg aacttagaaa
    37861 acaaggatat agcttagaga agctttcaaa taaatttggg ataagcaatt ctaatattag
    37921 gtacatgatt aaattgattg atcgttacgg aatagagttc gtcaaaaaag gaaaaaatcg
    37981 ttactattct cctgatttaa aacaagaaat gagtaataaa gtctgacatg aaggctggac
    38041 taaagataga gtttctcttg aatacggtct cccaagtcgt acgatacttc ttaactggct
    38101 agcacaatac aggaaaaacg ggtatactat tgttgagaaa acaagaggga gagtacctaa
    38161 aatgggacgt aagccaaaaa cgagacctga agagaggaca gaattagaac gtcttcaagc
    38221 agaaaatgag tacctgagag cggagaatgc catcctaaaa aagttaagag aactccgatt
    38281 gaaggaggag aaagaagaaa gacagaaatt gttcaagaat taatgactga attttcgtta
    38341 gctcttcttc taaaagtcat taaactagct cgttcgacct actactatca cttgaaacag
    38401 ctagataaac cagataagga ctaagagctt aaagctgaaa ttcaatccat ttttatcgaa
    38461 cacaagggaa attatactta tcgccgggtt catttagaac taagaaatcg tggttatctg
    38521 gtaaatcata aaagaattca aggcttgatg aaagtactca atttacaagc taaaacgcga
    38581 cagaaacgaa aatattcttc tcataaaaga cgttggcaag aaggcagaga atctcattca
    38641 aggccaattt gaaggctcta aaacaatgga aaagtgctac acagatgtga cagaatttgc
    38701 cattccagca agtactcaaa agctttactt atcaccagtt ttagatggct ttaatagcga
    38761 aattatcgcc tataatcttt caacttcacc caacttagaa caagtacaaa caatgttgga
    38821 acaggcattc acagagaagc actacgagaa tacgattctc catagtgacc aaggctggca
    38881 ataccaacac gattcttatc atcggttcct agagagtaag ggaattcaag catccatgtc
    38941 acgtaaggga aacagcccag acaatagtat gatggagtcc ttctttggga ttctgaaatc
    39001 ggaaatgttt tacggttatg agaagtcgtt tcagtcgctt aagcaattgg aacaagccat
    39061 tatagactat attgattact acaacaataa acgaattaag gtaaaactaa aaggacttag
    39121 ccctgtgcaa tacagaacta aatccttcgg ataaattaat tgtctaactg tttgagggca
    39181 gtacagaaaa atcttgatac tatgcgtttt ttgattgtga agacttttat ccacctatta
    39241 tacaaagtta agtatccatc acagtttaca attagccttc attacttaac ccattcacca
    39301 ttatagttta agtagtaacc acctactgtg gtattaacag ctaaagcacc tgaatcatag
    39361 gcatagtacc agttaccatt gacttggaac cagcctgttt tcatatctcc attacttgca
    39421 tttagatagt accaagtcga actttcctga taccaaccag ttgccatagc tcctgatgaa
    39481 cggagatagt accatttgtt cccaaggttt tgccagccag ttctcatatc accattttca
    39541 gcatctagat agtaccaagt tgagccttcc ttataccagc cagttgccat agctcctgac
    39601 gaatggaggt agtaccatct attacctaga tattgccaac ctgtttgcat aatgccagtt
    39661 gctggattta gatagtacca tggagctgct tttagcttct cttcatcata cgtaagaggg
    39721 taatccttaa cccaaccacg tgctagctct ccaaccgtca atctgttgat gcgcgaatca
    39781 aagccaccat ccttctgtaa ataataccaa tgaccctctt cataaatcca accagttttt
    39841 aaagtatgat aactacgctg atcttcaaaa taatagactc gtttctctgc ttggctatca
    39901 tattcttccc catgatgttt gttggtattc gtagcagttt ttgcttctaa aacttgcttg
    39961 ccaacaaatt cttgtaatac accatcttga ccaaaataaa accaatctgg tctaagagca
    40021 atctctattc ttggagaagg accaatcgta acccccttgt gtggagcagg tatatattgc
    40081 cagccgacaa ccatctctcc ggataaagga tcaaaataat agtacttccc atctatcact
    40141 ctccagtagg tttcttttag atcccccttt ttgtagtagg ttctaccatt ttcttggaca
    40201 aactgccatc cttcagaatc atctgcaaat actgtatttg tcgctaacaa accgaagaaa
    40261 aatgtggcta gtgcgatttg catcattttt ttcaaaagct tcattttata tatcctccaa
    40321 cattaaatcc actcaccaga tgaagcgaag ttataaactt taccatcgat agtttggcta
    40381 cctgtaacca ttgctccatt tgaattgaga taataccatt ttccatttac tttttgccaa
    40441 tctgtaagca tcttaccgga actgtctaaa taataccaag tatcatttat ctcctgccaa
    40501 cccgtttgtt tatcatgttg tctataatgg taccaactac catttatttt cttccatcct
    40561 tcaagagagt aacctttaag aaccgaataa ataaatggca aatttcgttc atttaattta
    40621 actgcactgt taatttgatt agctccatct cctaaagtat gcactcctac tacacggtga
    40681 ctagcatcat aaactgtaga tccactagac ccctctaaag tatcaacttg gtaatccaag
    40741 aacatgccat catcacttaa aacttgtttt ttatctgtat acatttgatg aattttaaaa
    40801 ttatatgatg gatagcctgt gatagtcaca gttattcctg tcaaattttt ttgactagta
    40861 ggaagaccca aagtccctaa ttttgcacca atgggctctt ctagaattaa taaagccaag
    40921 tcatattccc ttgcatcctt agaatttaaa tttctaaatt ccttcaaata acgaacttcc
    40981 tttactttga tctttccaaa tggttcttga cttggactaa cagccggaag aacataaata
    41041 tcatccgctt ctttcccata gtcatgtctg tagtagttgt gagccgctgt aattaacaca
    41101 ttatctttaa taaaattcgc acttcccctc ccttctacat tcattccatc ggctactcca
    41161 taatacttag ataaaaccat agctgaagtc gaatacggat actgtaaagt attatcaatt
    41221 ttaatcaaat catcattacc gataatactt ctgattgctt ttggtagtat gaaccatacg
    41281 ttggtgaaat ctcagataat gaagaatcat tagactctgg acctttttct agtgtctcac
    41341 ttacctcata ttcttcacct gtactagaaa taacactcaa agcagatact gtcgataact
    41401 ggctagccaa taaagtactc gcaataattg aaatacccaa ttttttataa acagttttct
    41461 tcattattgt atcctcctaa tgtaattata gcgtactatt ctaaatttct taatctacta
    41521 tagaatcaag aaatctacca ccttctttaa ataccctcca ttatcacata aacaggtaaa
    41581 cttttcaatt aatgactgcg cttttcaatc acgctagagg tacttgcttg cttctttgat
    41641 actaagttca gccattcttt ccttgttttt ctcaataaag catgttaccc aagtgggatt
    41701 cgttttggag tagtctcgca gagtccagcc aatggcttta ttgataaaaa attctgtttg
    41761 gttcaagtta tgaaggagaa tcttttccat taattgagta ttggtcttct cttttcttaa
    41821 caactggtgg tcaatagcga cacgtctcag ccagatatta tctgataggc tccattttat
    41881 actcaatgaa aatcaaagag caaactagga agctagccgc aggttgctca aaacactgtt
    41941 ttgaggttgc agatagagct gacgtggttt gaagagattt tcgaagagta ttaagattat
    42001 ttcttctagt tcagggtgtt catacaccaa actccctact actcgatcta ggatatctac
    42061 cgtgtcccac aaggattttg tcacgactaa ctgctctagc ttaggcaaat cggtttcctt
    42121 tagataagac tgcattgctt tcaaatagtt agcagccaca tattggtatt ttctaggatc
    42181 cttttcccag caagtgtctg caaaatccca atcgataatc tttgtttttt tcgcttctgg
    42241 aaaatatttt atagagttta tttctttcag gcaccgcaat acctagaaaa gaaaattgat
    42301 ggcgcatata ggcttccatg gaccttgctt ttttagagtc ttttgctgct tctagctcct
    42361 caagtaaatc tgctaaactc atctaaaact cctcttgccc caccaaatgg tgctgaaagg
    42421 catagacagc cgcctgggta cgatcgctga cttcaagttt ggcaagaata ttggacacgt
    42481 gggtcttgac cgtcttgaga gagataaaga ggtcatctgc gatgcgctga ttttcgtagc
    42541 ccttggcgat gagttggaga acatctcgct cacgcgcagt caattcttca tgaagttcca
    42601 tatgattgcg gtggtattca accttcttgc taacctcttg ctcaatggcc agctcgccag
    42661 cagctacctt actgacggca tgaagcaatt catctgcact agaagtcttg agcatatagc
    42721 ctttggcacc agcatctaag actggcatga ttttttcatt gtccaaataa gaggtcacaa
    42781 tcaaaatctt ggcttcaggc cattctttaa ggattgctaa ggtcgcgtca atcccattca
    42841 tctcaggcat gacaatatcc atgacaatga catctggacg cagttccaag gccaagtcaa
    42901 tcccttgaga cccgttggac gcctcaccca caacttctac atcgtcttgg aggtcaaagt
    42961 agcttttcaa gcccaatcgg accatttcat ggtcatctac tagtaaaatt ttcatcttta
    43021 ctcctttatc attccttatc taacagggga atacggatat caaccgccag cccttgcttg
    43081 ggagctgtca agagttgaac tgttccagcc atatcttcaa cccgctcctt gatatttcgc
    43141 agtccataac tcaagtcgtc taagctccct aactggaaac caatcccatt gtccaccacc
    43201 ttcagttgca attcaacatc tgtctgatag aggtagacat ctaggcaaga tgcctgggca
    43261 tggcggaggg tattgctaat caactcttgc aggatacgga agatatgctc ctcgattttc
    43321 ttaggcaatt tcgtcatatt ctgcttgaga ctaaccctaa gatcactctt gtcctcaagc
    43381 tcttttaaaa gaatttgaat cccttctatc aagctcttct gctccagttc aactggtcgc
    43441 aaatgcaaga gcaaaacccg caaatccttc tgggctgttt ctaaaatagc tgtgacactc
    43501 tgcaactggg tctgcatctt ttctctatcc aatttcaaag cctgctgact gatacccgat
    43561 aaaatcatgt gggccgcaaa caactcctga ctgactgtat cgtgcaaatc ccgagcaatt
    43621 cgcttccgtt ccttctcgat gatttcctct tcctgagcaa ggctctgatt ttcagctttt
    43681 tgaagagcct ctgtcaaaag gttaagttta cctgataagg acttgaaact ggcatccaaa
    43741 tctggatctg caacctgaac cacttcttgc cctgctaata aacgcttgag attagcctgc
    43801 atttttctta gagaaagctc ttcgatccct cgccaaaaca gggctaagag acaggtcatg
    43861 gacatgctga aaaccaacaa taaaaagaca aatttttctg ttttttcgac atcgtgcaaa
    43921 aagatagacc agtcaaaatc aagtatttcc agcaagctgt gggagaaaaa aaagacaaat
    43981 aggaaggagg tgagagcaat aatgacatag gcttgttttt tcatcctcta accacctcca
    44041 catcaccaat catagtggtc aagaaaatct tgacactctt gttactcttg agatagtctt
    44101 ttgtttcttg atgatagtgt tcattgcgga gggctcgctt gggctggttg aaaaaaatca
    44161 aatccccata gagacagtta acgctgagac tgacttccac atctacaggt acgatgattt
    44221 tggtcgttcc taccatcttt ctgaggataa tgacattgtc atgattggtt aagatgaccc
    44281 tctccagatg aatagtgtcc ttgcccatga agcgaaagag attgatatca tcgaattggc
    44341 aagtctggta gcttgaaaaa tgatgaagat ttccaaacca acgatttttc tccttcttaa
    44401 ccgtcacgac ctcttcaaaa accaaattgg tctgctcttt ttcctggttc atcatcgggt
    44461 aaagaagaaa gaggctatag ataaccgcaa caaaaatagc tagaatcaca aaaggattga
    44521 gcataacgat gaaaaagaag agaatggttg ccgctactaa aagaagatta tttccctctt
    44581 taccagtgta gtagcgaatc aaaagcaaaa agaggaatag tatcagcaga aaacgcgaaa
    44641 aatgctctga taccatcaaa atcagagctc ctgtcagaag acaggcttcg ataaataaaa
    44701 agattttaaa ttttctcata ggttcatcct ctcccttcta ttttatcaca attcaaaaaa
    44761 gtcacctcag tctgaggatg gaaaaaaggc gctggttacg cctttttcat ctgatccttt
    44821 gcttctttta attttccata aagaagatag tctacttttt gtagatctgc tatggtggca
    44881 cagttaaggg aacacataat caagcgtaga tctgctttcc agccttggac aatgccaatc
    44941 acttcttcaa ctgtgtaggt ttcaaccaat tccagaacgg ttcgtgacaa tcccacagcc
    45001 ttagcaccaa aaaccaagca cttaatcata tccagcggat tccgaacccc tccactaacc
    45061 aagagttcga ccttatcttt ccattcttgg gcattgagaa gggcctgcat ggtagactga
    45121 ccccattgat tgaggtaatc acgctggcca ctacgacggt tttcgatata ggcaaagctg
    45181 gtgccaccac gacccgatag gtccactgta cgaacaccga attcataggc tctttcgatt
    45241 gtcttggcat ccattccaaa gcccacttcc ttgaggacaa taggaacggg aatttgcttg
    45301 ctataatctg ctagatgcga ttgccagctt ctaaacttcc tttctccctc gggcatgagt
    45361 aattcctgca tgacattgac atgcacttgc aatagaacag gattcatctc ttctacagtc
    45421 tgaagtccta actcgacagg cttgtccaat ccaatattgg ttccaaggag gagattggga
    45481 tgactagact tgacagaaaa agaatcatcc gttggatttt tgagggctgc gctataagaa
    45541 cccgttacaa ataaaatacc acaggattcc gccacctgag ccagcttttg attgatttct
    45601 cttcccttat tacttccacc agtcatggca ttgatataaa aaggaaagtc ccactttcga
    45661 ccagcaaact ctgtcgaaag atcgatttca tccagattgt aaagaggcaa ggaagaatga
    45721 atcagctcca cctcatcaaa gctattatag gaacttttct gctcaagggc atagaggata
    45781 tgctcgtcct tacgatttgt cgtcatgtcc tatcctttct tgatataaga gctcaatccc
    45841 cagatcggcc caacgatttt ttaaggtttt ggttgattgc gcatcaaaac tcagggcgat
    45901 gccacagtca ccaccaccag caccactact cttggcaacg gtctgcaaat cttgactggc
    45961 ttctttcaac tgtctaagca aaggcgtgta aatatctgta ctcaagcctt ctaaaagctt
    46021 gctggctact tctacttgat cgataatctt ttctgatttc ccctgttcca aggcttctac
    46081 cagagaagtc accgtttctt ttgaggaagt taaaaaattt tgattgatat tttgcttgat
    46141 ttgctggacc atgtgactcg atacagccac ttccttggtc catcccacta agaaatcaca
    46201 ttctaaagtt ggtttcactt gtgaaattga aaagccccaa tcacgctcca gaactgtcgc
    46261 caagttttct tcttctaacc aagcagccac cttctggcga tcaaatgact ggtagagaac
    46321 caaatcctct gccacaatac aggcaaggtc gcccatggaa ccattgtctc ctcgcttaag
    46381 caagacagcg ctagtcagct tgaacaagag ctcctgatca acagaaacat catacagagc
    46441 cagtaaagcc ttgacaacca agacaacgac gctgccacta gaacctagac caaacttttt
    46501 cccttctcgt tccattttgc cacagatttc tagagaaaaa ggtcttaaat tctgaccacg
    46561 aacagcgagg aagtctccca tcaaagcaat cgtttcttga atcaagctat agtcaggatt
    46621 aggccttaag tccactgcga aatcaaacat atctgaatag atacggtagc tgtcagaaaa
    46681 agcaatctca gccctcatat agatgggaat atcctttatc aaagctaact gccctggctc
    46741 taaaatagca tattcacctg cccaatagag ttttccgcaa gttttaacag caatcatctt
    46801 gactcaaatc ctttgttttt gacacaatca agcgataacg atgaccgaaa atttctgata
    46861 aatgctccaa gtctttctcc tgacagaaga ccttaacatt gggaccagca tccatggtaa
    46921 agtagcaggc ctctcctttc tcacgaagct ggcgaacaaa ggccatagcc tcataagagg
    46981 catccgtcag ataagaaaag gctggactag cagtctttgt cgtagcatgc atagccaggg
    47041 catttttctc cgttaattct ccaatcttgg caaaatcatt ttccttgaga taaatcagca
    47101 tatcctgata gtccttctca gactgacgaa cccagtcgtc gaaagtcgtc gaggtttcca
    47161 cacaaagttt catcccgtca cggctagaga ttggtttttt cttgtcctct agcaccaaca
    47221 taatcatagc tagtttcaag tctgtctcta cagggtaaat ttctccacta tccttatccc
    47281 aggctcctag tggtccataa aaactccgag aagaagaacc tgaggcaaat ttggcttcct
    47341 gtgccaactg acttctatcc aatccaagct tgaaataagc attacaagcc ttgaccaggg
    47401 cggacaaacc actagaactt gaggacagac ccgctgccgt aggcatattg ttttgagtat
    47461 cgatacggac aaagccctca ccagctggac gataacggtc aataatctta ctcatcttgg
    47521 catgctcgac ctcattttgt agctgaccat tgatgtaaaa ttcgtcagct gttacattgg
    47581 ctggtaaagg cgacaaggtc gtctctgtat acatattttc caaagttaga gaaatactgc
    47641 tagtagcagg caccatctct ttttcttttt tctttcccca atatttgata atagcaatat
    47701 ttgcgtagga acgtactgtt acaggctctc tatccatgtc tgaacagctc ctttctcttc
    47761 taatctttct gctagttctt gtgcgtgtgt caaattggtt accaaggcta tgatacaacc
    47821 tcctagccca ccaccgctca tcttggcacc cagagcacca tggctaagag tcgtttcaac
    47881 caaaaagtct gcctcagggc tactgactcc aatttctttt aaatgtaaat gcgcttgact
    47941 gaggatttgt cccagtcctt cagcatcttt ttgtgaaatc gcaacttctg cttgctgggt
    48001 taattctccc aaggcatgca aaaacggtag ggcatccttg cccttatttt gaaccacttg
    48061 gatggcttca cgagtatgac cataaacacc cgtatcggca atcaccaaat aggcggataa
    48121 atccatctca agttctgtaa atcctacgtt cttgataaag cgaataggtt ggtcactaag
    48181 acaggtctta gcatccaaac cactaggatt catatgggca atcatttcag ctcgattgac
    48241 caagatttct agtacatcat gaggcagatc agcctgatag tagtcaaata ctgcacgaat
    48301 ggccgctatg ctgatagccg ctgacgaacc catcccccgt ttctcaggga tagccgagtc
    48361 aatctcacaa cgaatgcagg cttctgtgat attcaaatac tccagtgagg cataaaccgc
    48421 catggacaag gtatcctcct cataaaggcg ccaaggactc tctgcaggaa ctaccttaca
    48481 ggtcacctcc acctccaaaa gaggcaggga aatggcagga taaccgtaaa cgaccgcatg
    48541 ttcccctatt aaaattatct tactatgtgc ctgaccgaca ccaacttttt ttgtcatttt
    48601 ttccttttac tagacgaaaa aacgtcttat ttttcataca agtattaatt ctttcctatc
    48661 tattttatta tattttcaca aaaaaagcga ttgtttccat tcacaatcgc ttctttcatt
    48721 attgaaccca ttcgccatta tagttgacag aatagccatc tacggtcgta ttcactgcca
    48781 aggcacctga gcgctataag cgtagtacca tctgccattg acctggaacc aacctgtcgt
    48841 catagaacga cgaaagaaac tccataccat taagtaaaga ggaaagtcgt gagggagcat
    48901 gcgccattga caacctgttt tagtgacgta caaagtctca ttaacaagta ctcgtttcgg
    48961 ccatttatag gtgcggtgtt tggagaaata gggttcaatc ttcgcccatt cttgatcgtt
    49021 taaatcagta tcatatgctt tgcgtatcat aactctagct taacattttt ttgtgaatac
    49081 aggttctaaa taatcgacca cgaaaatttc ttaagtggaa aacgccttat gaagtatgct
    49141 acgggaaagt tatgcactta atttgacaat tcaagatgta aaaatat
//
LOCUS       DAVKNL010000010        47503 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVKNL010000010 DAVKNL010000000
VERSION     DAVKNL010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 47503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47503
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.010"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(<1..334)
                     /locus_tag="VW411_000528"
     CDS             complement(<1..334)
                     /locus_tag="VW411_000528"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C family protein"
                     /protein_id="HEU9793315.1"
                     /translation="MLVNEMEITDHRVDNLFEKGKNEIKDSIGTNSVLNKKIILQKIR
                     KLSNQPSGYWIGSLDERFLDHAIINQIDVTSEQIVLMSDGFYEFYQNNQNKTFEELIK
                     MRFNSSAID"
     gene            complement(494..790)
                     /locus_tag="VW411_000529"
     CDS             complement(494..790)
                     /locus_tag="VW411_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793316.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYKLTEYSKMK"
     gene            complement(774..2933)
                     /gene="pfbA"
                     /locus_tag="VW411_000530"
     CDS             complement(774..2933)
                     /gene="pfbA"
                     /locus_tag="VW411_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEU9793317.1"
                     /translation="MLKIVKKLEVLMKYFVPNEVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEVVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKIQNNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVIN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYI
                     SFIKSNANGKEPVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVI
                     DSNQKNGEFNSSKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVK
                     QKLPKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            complement(4063..5289)
                     /locus_tag="VW411_000531"
     CDS             complement(4063..5289)
                     /locus_tag="VW411_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEU9793318.1"
                     /translation="MPNYNIPFSPPDITEAEITEVVDTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSIYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(5332..6024)
                     /locus_tag="VW411_000532"
     CDS             complement(5332..6024)
                     /locus_tag="VW411_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU9793319.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            complement(6197..7948)
                     /locus_tag="VW411_000533"
     CDS             complement(6197..7948)
                     /locus_tag="VW411_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9793320.1"
                     /translation="MQNKQEQWTVLKRLMSYLKPYGLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGFSIG
                     ITVGTMYAFIQYINRLFDPLIEVTQNFSTLQTAMVSAGRVFALIDERTYEPLQENGQA
                     KVQEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQETSDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLALGG
                     TYHKMYSLQAGAMADTL"
     gene            complement(7938..9683)
                     /locus_tag="VW411_000534"
     CDS             complement(7938..9683)
                     /locus_tag="VW411_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9793321.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALILVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYTTSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLLAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKEMRKDKTTIITAHRLSAVVHADFILVLQNGQIIERGTHEDLLALDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(9889..10266)
                     /locus_tag="VW411_000535"
     CDS             complement(9889..10266)
                     /locus_tag="VW411_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEU9793322.1"
                     /translation="MKRLIDGKPTLLIKNGNIDPEACRSVGLSASDVSLKLRSQGIFQ
                     MKQVKRAVQEQNGQLIVVQMGDENPKYPVVTDGVIQVDVLESIGRSEEWLLDNLSKQG
                     HDNVANIFIAEYDKGAVTVVTYE"
     gene            complement(10338..10794)
                     /locus_tag="VW411_000536"
                     /pseudo
     CDS             complement(10338..10794)
                     /locus_tag="VW411_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            10928..11755
                     /gene="xth"
                     /locus_tag="VW411_000537"
     CDS             10928..11755
                     /gene="xth"
                     /locus_tag="VW411_000537"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU9793323.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDAKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            11767..11910
                     /locus_tag="VW411_000538"
     CDS             11767..11910
                     /locus_tag="VW411_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEU9793324.1"
                     /translation="MDYNAVIPELLVSNIEQSRSFYCGLLGFRIEYQRPEENFLFLLK
                     SVN"
     gene            12021..12149
                     /locus_tag="VW411_000539"
     CDS             12021..12149
                     /locus_tag="VW411_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEU9793325.1"
                     /translation="MKANYPIYRPLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     regulatory      12532..12627
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            13018..13599
                     /locus_tag="VW411_000540"
     CDS             13018..13599
                     /locus_tag="VW411_000540"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEU9793326.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFATTGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAEKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDCFENGQVVFKEADL"
     gene            13599..14861
                     /locus_tag="VW411_000541"
     CDS             13599..14861
                     /locus_tag="VW411_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEU9793327.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVATLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(14911..15372)
                     /locus_tag="VW411_000542"
     CDS             complement(14911..15372)
                     /locus_tag="VW411_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpnD"
                     /protein_id="HEU9793328.1"
                     /translation="MKTKQLVASEEVYDFLKVIWPDYETESRYDNLSLIVCTLSDPDC
                     VRWLSENMKFGDEKQLALMKEKYGWEVGDKLPEWLHSSYHRLLLIGELLESNLKLKKY
                     TVEITETLSRLVSIEAENPDEAERLVREKYKSCEIVLDADDFQDYDTSIYE"
     gene            complement(15369..16133)
                     /locus_tag="VW411_000543"
     CDS             complement(15369..16133)
                     /locus_tag="VW411_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-2 restriction enzyme DpnI"
                     /protein_id="HEU9793329.1"
                     /translation="MELHFNLELVETYKSNSQKARILTEDWVYRQSYCPNCGNNPLNH
                     FENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGAYATMMKRVQADNNPNFFFLTYT
                     KNFEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDG
                     QVRDPEKVTKEFKQGLFLRKSSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKN
                     IFVKNNHIKEKIRQQLQILRDKEIIEFKGRGKYRKL"
     gene            complement(16314..16718)
                     /locus_tag="VW411_000544"
     CDS             complement(16314..16718)
                     /locus_tag="VW411_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU9793330.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVRI"
     gene            complement(16788..18269)
                     /locus_tag="VW411_000545"
     CDS             complement(16788..18269)
                     /locus_tag="VW411_000545"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEU9793331.1"
                     /translation="MTLVNKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGAELMDLVTPCPSQVNRDFWATYAHSPE
                     QAIEDFYQLSQKNDYIKLKAIARNIAYRVPSDYGELEITINLSKPEKDPKEIVAAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVDQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKAFRFAGFEQVKAGIVKWPMSVLRLTSDSKEDLINLADKILQEWRQYSDPAVQ
                     ILAETDRTPHHTITPIARKRDGQFELDLVLRDNQTSAEYPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFARVLEDAGVYKQTEQGQTAFMRFVEQVGILLD"
     gene            complement(18288..19466)
                     /locus_tag="VW411_000546"
     CDS             complement(18288..19466)
                     /locus_tag="VW411_000546"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEU9793332.1"
                     /translation="MAQHLTTEALRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVSLDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQVALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            19631..20638
                     /gene="galR"
                     /locus_tag="VW411_000547"
     CDS             19631..20638
                     /gene="galR"
                     /locus_tag="VW411_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEU9793333.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFKNYSQARGIYHDELVFQGR
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(20684..21721)
                     /locus_tag="VW411_000548"
     CDS             complement(20684..21721)
                     /locus_tag="VW411_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU9793334.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGADSATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(21722..22075)
                     /locus_tag="VW411_000549"
     CDS             complement(21722..22075)
                     /locus_tag="VW411_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU9793335.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(22319..23209)
                     /locus_tag="VW411_000550"
     CDS             complement(22319..23209)
                     /locus_tag="VW411_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEU9793336.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            23342..23878
                     /locus_tag="VW411_000551"
     CDS             23342..23878
                     /locus_tag="VW411_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU9793337.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNLPTSYLQHLVMSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            complement(24045..24851)
                     /locus_tag="VW411_000552"
     CDS             complement(24045..24851)
                     /locus_tag="VW411_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEU9793338.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(25196..26716)
                     /locus_tag="VW411_000553"
     CDS             complement(25196..26716)
                     /locus_tag="VW411_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEU9793339.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPNLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLAALIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLVQKEKENLVIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGKTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATIEPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            complement(26709..27437)
                     /locus_tag="VW411_000554"
     CDS             complement(26709..27437)
                     /locus_tag="VW411_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9793340.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKAPATDFVADLFGGSVHD"
     gene            complement(27452..28216)
                     /locus_tag="VW411_000555"
     CDS             complement(27452..28216)
                     /locus_tag="VW411_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU9793341.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFREDENG
                     CIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHDIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(28220..28663)
                     /locus_tag="VW411_000556"
     CDS             complement(28220..28663)
                     /locus_tag="VW411_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9793342.1"
                     /translation="MKDGHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKSQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            complement(29345..29641)
                     /locus_tag="VW411_000557"
     CDS             complement(29345..29641)
                     /locus_tag="VW411_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU9793343.1"
                     /translation="MVEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFVKREEAKYRGTATSALMYEELFKML"
     gene            complement(29663..30727)
                     /gene="pepA"
                     /locus_tag="VW411_000558"
     CDS             complement(29663..30727)
                     /gene="pepA"
                     /locus_tag="VW411_000558"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEU9793344.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            31128..31829
                     /gene="galE"
                     /locus_tag="VW411_000559"
     CDS             31128..31829
                     /gene="galE"
                     /locus_tag="VW411_000559"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9793345.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYQVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            32252..33211
                     /locus_tag="VW411_000560"
     CDS             32252..33211
                     /locus_tag="VW411_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9793346.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTHKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            33201..34157
                     /locus_tag="VW411_000561"
     CDS             33201..34157
                     /locus_tag="VW411_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEU9793347.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFVLLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFILANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRSRQ"
     gene            34154..34906
                     /locus_tag="VW411_000562"
     CDS             34154..34906
                     /locus_tag="VW411_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9793348.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            35002..35967
                     /locus_tag="VW411_000563"
     CDS             35002..35967
                     /locus_tag="VW411_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9793349.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELTKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(36192..36434)
                     /gene="yidD"
                     /locus_tag="VW411_000564"
     CDS             complement(36192..36434)
                     /gene="yidD"
                     /locus_tag="VW411_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEU9793350.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDRFSLKRNQEGE"
     gene            complement(36434..37156)
                     /locus_tag="VW411_000565"
     CDS             complement(36434..37156)
                     /locus_tag="VW411_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU9793351.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            complement(37143..37712)
                     /locus_tag="VW411_000566"
     CDS             complement(37143..37712)
                     /locus_tag="VW411_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEU9793352.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            complement(37731..38459)
                     /locus_tag="VW411_000567"
     CDS             complement(37731..38459)
                     /locus_tag="VW411_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEU9793353.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLTFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            complement(38459..39199)
                     /gene="xerD"
                     /locus_tag="VW411_000568"
     CDS             complement(38459..39199)
                     /gene="xerD"
                     /locus_tag="VW411_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEU9793354.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            complement(39190..39651)
                     /locus_tag="VW411_000569"
     CDS             complement(39190..39651)
                     /locus_tag="VW411_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9793355.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            complement(39648..40169)
                     /locus_tag="VW411_000570"
     CDS             complement(39648..40169)
                     /locus_tag="VW411_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU9793356.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            complement(40145..41116)
                     /locus_tag="VW411_000571"
     CDS             complement(40145..41116)
                     /locus_tag="VW411_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEU9793357.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSIYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            complement(41113..41907)
                     /gene="racE"
                     /locus_tag="VW411_000572"
     CDS             complement(41113..41907)
                     /gene="racE"
                     /locus_tag="VW411_000572"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEU9793358.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            complement(42115..42363)
                     /locus_tag="VW411_000573"
     CDS             complement(42115..42363)
                     /locus_tag="VW411_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEU9793359.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            complement(42619..44244)
                     /gene="treC"
                     /locus_tag="VW411_000574"
     CDS             complement(42619..44244)
                     /gene="treC"
                     /locus_tag="VW411_000574"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEU9793360.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEKMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWTPFGDTGKYYLHLFDETQA
                     DLNWRNSNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKYINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            complement(44422..46389)
                     /gene="treP"
                     /locus_tag="VW411_000575"
     CDS             complement(44422..46389)
                     /gene="treP"
                     /locus_tag="VW411_000575"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEU9793361.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QGVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWHKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGLAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            46574..47284
                     /gene="treR"
                     /locus_tag="VW411_000576"
     CDS             46574..47284
                     /gene="treR"
                     /locus_tag="VW411_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEU9793362.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
BASE COUNT        13913 a        10784 c         8860 g        13946 t
ORIGIN      
        1 gatcaatagc agaagaatta aatctcatct taattaactc ttcaaaagtt ttattttgat
       61 tattttgata gaattcataa aagccatcgc tcattaaaac aatttgttca ctagtaacat
      121 ctatttgatt aataatagca tggtctaaaa atctctcatc caacgaacct atccagtacc
      181 cactcggttg attagataat tttctgattt tttgtaaaat aattttttta tttaaaacac
      241 tatttgtacc aattgaatct tttatctcat ttttcccttt ttcaaataag ttatctactc
      301 tatgatcagt tatttccatt tcgtttacta acatgacgca gtcacctagc atcatatact
      361 ccaacttttt ttctgaaagt ttagcaaata ttggtaagcg ataatatagt atattgaaac
      421 tagaatagta cacctctact tctaaaacat tgttagaaat cgatttgact gtcctgattg
      481 atttgtccta ttattatttc attttactat actctgttaa tttatatgag tttaaaccga
      541 tttcatcttt aacctcgagt aaagcagttt caaatatttg tttaagagtt tttgattctt
      601 tacaattaac cgacaaactt tctgataaaa tatgtacaac ttctgagact gaataaccta
      661 tctcctcttt agaattatat aaatctgtag ctccaccaat aatccaaaaa tactgatttt
      721 gtgaacctac aatatcctca ttttctacgg aacttccttg tatcgaacaa attttattta
      781 tctttaccat aatacttcaa cccttttagt gtcaaaagta aaccaattcc tgtcactgtt
      841 aagaatagtt ccataatctt attcgaacca gtctttggta atttttgttt tacatctact
      901 atttctttag atttattaat atgattttca gtttctctgc catctccaac tattttatag
      961 tttacttctt ctgtcttatt atcttgttta ttgtcgatct tgtcattcat ttgtctatta
     1021 tctttacttg agttaaactc tccgttcttc tggttactat caattacatt atttgaatta
     1081 gattgttttt cctctttgtt tttttctttt tcgtttttat cacttaaatt atttgttaca
     1141 attttgtaaa gcccattctc cgttacaata ttgaaattac catcgctatc acgtataaca
     1201 ggttctttcc catttgcatt agatttgatg aatgatatat acttaccgga taaattataa
     1261 aattggttat ttaaaacggt tattttaccc tttgaatcct caataacaat tccttcttta
     1321 cccccgaata tactattctc tgagactact aaattatcac taactcgtaa taattcaata
     1381 tttggttgtt ctgtttcctt agaattatta ttaattacat tttttgttac agtaatatct
     1441 gatacttttc ctaaatattc tgcactatct tttgcaactc gtatcgcttt tgttttagga
     1501 tcggcaatat taaatatatt ttcggcgata accacctgtt tatttaaatc taataggtct
     1561 ttagtgtttt tatagctata agcatttact aaagctgctc cgctttctcg ataatgtaca
     1621 ctctctcctt taactttctt atcaaagcga tttcctttga ttaatacatc agtgaatcct
     1681 gtaaaacgta cacctgcata catcatgtta tcaaaatgat tattttgaat tttaatatta
     1741 gaggggttct gtgtcgacaa tgtttgatag tgtgtgccaa ttgctgttac taattcccca
     1801 gatttatcac ttttgccaaa ataggaattt tgaatagtca cattttcaga ttttttccca
     1861 tcatcattca aggcataagg aaaacctttt ctagttaatg gttcaatctg aatgctctcc
     1921 ttacttatga tttgcccatc cttcatcgtt ttgggtaagg cttgcccaag aaaacgagaa
     1981 ttatcaacta atacattttt cgaacctgca atttgaatag catgcccttg ataactatcc
     2041 ttgaatgtta cattttttat agttacgtta tttgaattcc caatagcaaa tgcacctgaa
     2101 gaatttataa gtggtagatt ttttgcttta gttccttctt cattcaaagc accgttcata
     2161 tcaatcgtac caccagaata actaatatct tctgttgggc cccattctac ttgcgcacca
     2221 tcatccgtaa ataaacctgt cataaaaaca atggaagggt gattcttaat atttatacca
     2281 tttagaattg tagctttctc attcaattct aagtgtgtat gactttttaa aaaaacaatt
     2341 tcttttacta aataagttcc ttcaggaaaa tatacatttc ctccacctag cccttgagct
     2401 gcagcatcta ttgcatcttg aattgcttgt cgatcatcat gaatcccatc acctaccgca
     2461 ccataatctt ttacacttaa agcagtatct tcaaattctt ttttgataat tctgtcactt
     2521 ataacatctt ccttataact cttattttca ttagtagatg ttactgtaga ttcaactgtt
     2581 ctgttgtcta attgtgaatt ggaacgaata gtaccattag agctgatact attaacactt
     2641 aacgaattat ctaaaccatt agtggttgaa ttagaggcaa tcatttcagc agaacgattc
     2701 tgattaacag ttggtgaatt atctaaatca ttagtaattg cattagaaga ctcttttgta
     2761 gccatcggtg aagaactagt aacaacttca tccgataaaa taccttggct tcccaaaatt
     2821 gaaattgcca atagtaccga gcaagtcccc acctttaatt tacgaatact gaatacctca
     2881 ttaggaacaa aatatttcat cagaacctcc aattttttta ctatttttaa caattgatta
     2941 ttacttttct taaaatagat agaatgacac cgctgtcatt atctttatca tatattttcc
     3001 aaattgaatt gtcaaggaat aacacaagaa aatattaaaa tttatactct tcggaaatct
     3061 cttcaaacta ggtcagtgcc gccttgccct aagtctaggt tactgacttg gtcagttcta
     3121 tagtagactg aaactaaaat agtacacctc tacttctaaa acattgttag aaatcgattt
     3181 gactgtcccg atcaatttgt cctattctca tttcatttta ctatatctac aacctcaaag
     3241 cagggttttg agctatctat ggccaacttc ctagtttgct ctttagcctt ccattcaaaa
     3301 gtgatgctat ctttcttagc ttgaatataa aatatcccat ttactatatt taaatttgtt
     3361 gatatgttgt aaattataaa tgaaagaaca aataccttgt catacaagca ttcgccaggg
     3421 atatttacct gaatatcgct catatggcgg attgaaacta aaacactaca atatatttct
     3481 taaatagacc ttctataaaa gctagattta acctcgataa aaatagatgc agtgcttcta
     3541 aaatactgga atagggcata taggtaatcg tagacaagtg ttttatagat tgtctcgcag
     3601 gagatctaat aatgtatagc aagcatcact aaacatatag aatatttgta ttattataat
     3661 atcaacatgc tataaattct atcatgcaaa agaatgacta gaacatgaaa gacaggcaca
     3721 aaattttgaa caatattctg aaaacaaaaa aagacgcgag tttccacaat ctcactcatg
     3781 gaaactcacg tctttcaaaa aatagggtga attgaaaact agaacaagac actagatttt
     3841 ctaaatgatg tctagtaagg attctaaatg ctacactaaa ttgctcacat cttatttcaa
     3901 accacgggac tcccacaaga aaggaaaatc ctataaaaat tctctctctg aggagaaagg
     3961 ggcctcaact acaggtgtaa actattatca attgaaaaca acagttttta cactcaatta
     4021 gactttttag gggcaggatc actttcgctt gactgtagtt tgtcattttt ttgataaagt
     4081 tagcactttt tcagaaactg ttttgaaagt ctcaatgata tagtctactt cttcatcgct
     4141 taatttagta tgaagaggga gggtaatttc attctcaaag aaggcatagg ccttaggata
     4201 gttcgtcata tcaaatccaa gattcttata ggctgtcaag agaggaagcg gtttgtagtg
     4261 aacattactt gcaattcctg ctttagccaa ttcttggatg atgaggttgc gttcttctag
     4321 gcttgctcct tctacacggg tgatgtagag gtggcgtgaa gattcgacag tttcagtctt
     4381 gtgtgccaaa ggatggatgc gagaacctgc aaaaccacta tcatagcggt ccacaatgtc
     4441 cttacggcgt tgcaacaaac ttggatagcg gtccaattgt accaaaccaa gtgaagccat
     4501 gatatcggtc atgttgcact tataggctgg tgtaacgata tcgtattccc atgaccccag
     4561 ttgcatcttg gcaagagcat ccttagtttg cccgtgaagg gaaaggattt ggaattcctt
     4621 gtacatctct tcgtcatcaa tcactggatt ggctttccaa gtcgcacttc caccttctgc
     4681 cgttgtaaag ttcttaactg catggaatga gaaggaagta aagtcagcga tagaaccaga
     4741 aggttgtcct ttataaatag atcccaaagc gtgggcacta tcagagacaa tgacaatacg
     4801 gttaaaggcc ttttgccact tgcttgaagc ggtaaagaag tcacgttttt tctccacgac
     4861 ttggaacaaa cggtcataat cgcaaacaat ccctgcgagc tctactggaa taatcacctt
     4921 agttttctca gtgatagctt gctcaagcag gtcatagtcc atctcaaacg tatctgcttg
     4981 gatatccacc atgacagggg ttgctcccac gtgcgtaatg acactacatg aagccgtata
     5041 ggtcatggct ggaacgatga cttcatcacc aggtcccact tccaaaacgc gtaaaatcaa
     5101 ctccagagcg gctgtcgcag agttgagaca aacagtctta ggtgtctgtg tgtaaagaga
     5161 caagcggcgc tccagttctt ttgttttagg acctgttgtg atccaaccag aacgcagggt
     5221 atccactact tcagtaattt ctgcttctgt gatatcaggc ggtgaaaatg gaatattgta
     5281 atttggcatt gatttgctcc ttttatctgt actcattttc ttatgactac tttattttag
     5341 tacctcaaac acggtttgaa acatgatttt gatgtcccca aagaaactaa actctcggag
     5401 ataggcgagg ttatagcgca tcttttcagg aagaacatgc tccacatagg cctgatcaac
     5461 tgacagacct ttctccgtca tttgactgat aattgtgtcc tcatccttgt agttgatgct
     5521 ggctggagag gtaatccctg cttgcaagag caaggttgcc atcatttcag ggctatactg
     5581 ctctgtataa cgtggcactt caggtcgtgt accgacaaag gacatctcac ctttaaggac
     5641 attgaccaac tgaggcagtt cgtccaaacg gacacgtcgg atgaaatttc caaccttggt
     5701 aatgcggcta tcgttagcag aagtcaccag acttcctttt ttatccgcat ccgtcaccat
     5761 ggtacgaaac ttccaaatct tgaaccgacg gttgtactgg gtcacacgct cttgcttgta
     5821 aatcactggc cctttgctat ccaacttgat ccaaatgctc aagatgagaa agatgggaga
     5881 ggtcagaacc agtaagacca aggccaaaac ccagtccaag caacgcttga aaatcagcga
     5941 acccttcctt ttagagacaa gctggtagta agactcaacc tcgcttgatt tcatttccac
     6001 aggcaagtct tcccatttca gcatgctgtt ctcctttgtt tttattatac tatttgtcaa
     6061 catttttcat tataccacaa aatggaaaag gcggtgaaag aaatctgtaa tttgaaggat
     6121 ttccttctag tactcaatga aaatcaaagt acagctttga gattgcagat aaagctgaca
     6181 tggtttaaag agattttcaa agagtatcgg ccatggcccc tgcctgcaaa ctatacatct
     6241 tgtgataggt tcctcccaga gccaagagtt cctcatgggt tccactctcg ataatgcgtc
     6301 ccttatccaa gacatagatg cagttggcat cttgaatagt agaaaggcgg tgagcgatag
     6361 caatagttgt tcggccctgt ctcatcttcg ccagagaagc ttgaaccaag ctttctgttt
     6421 cagagtcaat attggctgtc gcttcatcca aaatcaggat tttaggctgg ctggcgactg
     6481 ttctagcaaa ggcaagaagc tggcgttgcc cagtagagaa gctcgaacca cgctcggaaa
     6541 caggggagtc gtacccctga ggaagttctt gaataaagga atctgcatcc acaaaggctg
     6601 ccgcagcctg aacctgctca tcactggttt cttggtacat ggcgatattg gacttaatag
     6661 ttccatgata gaggaagggt tcctgcaaga ccaaaccgat gttttttctc agctcttctt
     6721 gactgaaatc cctgatatcc acatcatcca agagaactct ccctgactgg aattcataaa
     6781 agcgcatgag gacattgata atagacgatt tccctgaacc tgtatgacct acaaaggcaa
     6841 tggtttcacc cttattaaca gagaaagaaa tgtcatccag aatcggatgt ttaccgtcat
     6901 atgagaaaca cacatgttca aaacggatat tgccttcttg gactttggct tgcccatttt
     6961 cttgaagagg ttcataggtc ctctcgtcta tcagggcaaa gacacgacct gcagaaacca
     7021 tagccgtttg cagagttgaa aagttttgcg tcacctcaat caaggggtca aaaaggcggt
     7081 tgatgtactg gataaaggca tacatggtcc cgaccgttat cccgatagaa aaaccacggt
     7141 agccaaagta ggccatcaag actgcatagc ctagaagttt cagcaaactc atggcaggtc
     7201 tcaaaaagag ggcatccaag gctacagaac ggttggcgta gaccaagtgt tcttggttga
     7261 tttcatcaaa ttctgcctgc aggcgcttct cttgattaaa ggcctgaata atcctgattc
     7321 cctcgatatt ctctgccagc ttactattga tatctgacaa gagacttctg gttttctcga
     7381 tgattttcac tgactttttt cgatagagat tgaccaaaag gaaaatcaaa ggaagaaaga
     7441 gcaagactaa agccgtcaaa cgaaaatcca gcaccaacat ggtataaagg gttgtcagaa
     7501 agataaaaac tgctgagata aagctggata aaatcccaga aaacatatca ctaatcgtct
     7561 cggtatcgtt ggtcaaacga gaaacgatag aacctgctgg cgtcttgtca aagtaagaca
     7621 tgcccagttt ctccatattg gcaaaggcat cccgacgaat atccctaaca atactgtaag
     7681 acacgcgcgc aaagagaaga ttgccgacat actgaactac agtttgtagg atgtagagac
     7741 catagtagac cagcaaaacg gtaacggcta gttggttaag attgctgaga tactggtcga
     7801 taaagtggga agccacgagg ggtatgacac ttttaatgac cgtcgtcgct aggagaaaac
     7861 tgagtgccaa aaaggtcagg agtccataag gcttgagata agacatcaag cgcttcaata
     7921 cagtccattg ttcttgttta ttctgcatct tcttctcctt tcatttccaa ctgctgagac
     7981 tggtaggttt gggcatacca gccatccaaa gctagcaagt cttcgtgcgt gcctcgttcg
     8041 ataatttgac cattttgtag aactaaaata aaatctgcat ggacaacagc actgaggcga
     8101 tgggcagtga taatggttgt cttgtccttt cgcatctcct tgaggttgtc gataatcgca
     8161 tactctgtct tggcatctac ggcggataag gaatcatcca aaatcaagat atcagggtct
     8221 aaaatcatag cccgactcat agccaaccgt tgcttttgac caccagaaag actgactcct
     8281 ttttcaccaa tcagcgtatc aaatccttga ggcatgtcta caatatcttg gtaaacccgg
     8341 gctagcttag tagcttcctc gaccgctgaa aggggcaagt taggattgcc aaagcggata
     8401 ttgtctagga ttgaagtcgc aaaaagaaac tggtcctgag gaacatagcc catgagactg
     8461 cgaaggtctg tcagacgata gtcccgaata tcgtgaccgt ttagataaat ggcaccctta
     8521 tccacatcgt attcacgcaa gaggagcttg attaaggacg ttttcccaga gcctgtctgc
     8581 ccaaccaagc ccagtgtttg cccttttgcc aaactaaagt gaatatccgt cagtgtttcc
     8641 tcattttcaa aagcaaagct gtcaatggca tactccaaac gcccattttc aataccatcc
     8701 agaggaaact cagggtcttg tacaggagat tcctgagaca aaagattttc aatccgctgg
     8761 taagaaacct tccctcgctg agtagtatta aagaggaaac cgatggccag aagaggccag
     8821 accagcatat ccaaatagct gataaaggtg actagattcc caactgtaat ctgcccttcc
     8881 tgaaccatca aggagccaac caaaagcgtt aaaacatagg acgaaccaac aaacaagaga
     8941 accatagggt caaagagact atcatatttc atggtttgca ggttcttttg gaaggttaat
     9001 tcattgactg cctgaaaaga cttcaactcg tctgcctgat aaccgaaaga cttggtcact
     9061 ttgatacctg atacggactc ctgtaccttg ttattgagtt cagaaaaagc agcttgggat
     9121 tcgccaaagg ccttatgagt ctttctccct aggcgactag tcgtataggc catgaaaggt
     9181 agggggagaa tggcaacaag agtcatctgc catgagatgc taaagagcat ggtcaacaaa
     9241 gtcaccagag ccgtgataga ggcatccacc gcagacatga caccgccacc tgctaaacga
     9301 gtcaaggcat tgatatcatt ggttgcgtgt gccatcagat cacccgtccg ataggtttga
     9361 taaaaggctg acgacatttt tgtgaaatgc ttaaacaagc gagaccgcat gatctgtccc
     9421 aagcaataag aggtcccaag gatatacata cgccacacat agcgcaaata gtacatacca
     9481 aaggctgcaa gtagcaagta aaataggcta agaaggaggt cctgctgggt taattgcccc
     9541 gatgtgatgg catcaatgac ccgccccata accataggag gaatgagatt gaggacggaa
     9601 accaagatca gggccacaat tccgactaga taacggcgtt tttctaactt gaaaaaccac
     9661 caaagttttt gaataatgga cataaaatcc ctttctgatt gcaaatggaa acctgaggcc
     9721 aatactcaat gaaaatcaaa gagcaaacta ggaaactagc cgcaggctgc tcaaagcact
     9781 gctttgaggt tgtagataga actgacgaag tcagtaacct acatacggca aggcgacgtt
     9841 gacgcggttt gaagagattt tcgaagagta caagacccca ggtttttctt attcataagt
     9901 tacgactgta acagcaccct tgtcatattc agcaataaag atattggcta cattgtcatg
     9961 cccttgttta ctgaggttat caagcaacca ctcttcgcta cgaccaatcg attccaagac
    10021 atctacttga atcacaccgt cagtcacaac tggatactta ggattttcat ctcccatttg
    10081 cacaacgatg agttgcccat tttgctcttg cacagctcgt ttgacttgct tcatctggaa
    10141 aatcccttgg ctacgaagtt tgagggatac atccgatgca gacaaaccaa ctgaacgaca
    10201 ggcttctggg tcaatattcc catttttgat aaggagagtt ggcttaccat caatcaaacg
    10261 tttcacaaag tgaacattgt tgttgagcta tttgagagtc aagaccagtt tgattaaaat
    10321 ttcgatataa ttaagtgtca tttgttcaca tccaccagtt caattgcgtc tgtcttatac
    10381 aattcgactt tctctaacag gtacttgtct ggctcacttc catttagggc acgataatag
    10441 cgatctccca ccttgataag tgcgccatct gtgtttgtgg aagtattaac atagacttct
    10501 gacttgtcta ctcacaaatc tttggaaaca acctcgatga aatgaagtga agtttgaaat
    10561 ttattgttag aaacttgatt gttttgataa gtcgtgatgc tgaccaaaag cagagctaaa
    10621 taaggccaga tctgaaatca tgaccaactc acgaaattcg cttccctttt tattatggta
    10681 tgccttgaaa gcaaaaaaaa ctgtcacagc taatagaaca atcccaaagc caaccatgat
    10741 accattttgc tgaccgattt ggctgagtac atagtcataa gagtagaatt tcatgtattt
    10801 tcactccctt tcataaaatc attcttaatt ataaacgaaa gagagtaatt tttcaaacca
    10861 tacgttgggg aaatagatct ttttttggta taataaaatc tataatctga atgaaaaagg
    10921 taactttatg aaacttatct catggaatat tgattcccta aacgctgccc taactagtga
    10981 ctcagctcgt gccaaattgt cccaagaagt cctacaaacc ttggtcgctg aaaatgctga
    11041 tatcattgct atccaagaaa ccaagctttc tgccaaagga cctacaaaga aacacgtgga
    11101 aattttagaa gaactcttcc caggctacga aaacacgtgg cgctcttccc aagagcctgc
    11161 ccgtaaaggc tatgctggaa ccatgttcct ttataagaaa gaacttacac ctactatcag
    11221 cttcccagaa atcggtgccc cttctaccat ggacttggaa ggtcgtatca tcactctaga
    11281 atttgatgca tttttcgtaa cccaagttta cactccaaac gctggtgacg gtctcaaacg
    11341 cttggaagaa cgccaagtct gggatgccaa atatgctgag tatttggctg aactagacaa
    11401 agaaaaacca gtccttgcga ccggtgacta caacgtagcc cacaatgaaa tcgaccttgc
    11461 aaatcctgct agcaaccgcc gttcacctgg atttactgac gaggaacgtg ctggatttac
    11521 caaccttttg gcaactggat ttacagacac cttccgccat gttcatggcg atgttcctga
    11581 acgctacact tggtgggcac aacgcagcaa aacttctaaa atcaacaata caggctggag
    11641 aatcgactac tggctcacaa gtaaccgtat cgctgacaag gtcactaaat ctgatatgat
    11701 tgactcaggt gctcgccaag accatacacc gattgtcttg gaaattgact tgtaaggatt
    11761 ttctatatgg actacaatgc ggtcattccc gagttgcttg tatcgaatat cgaacagtcc
    11821 cggtccttct actgtggttt actgggtttt aggattgaat accaacgtcc agaagaaaac
    11881 tttctctttc tcttgaagag tgtcaactaa tgttagagga gggaacaaag gatcaactag
    11941 ctgaactgac ctatcccttt ggtcgtggag tcaatctctc ctttggcata aaggatgttt
    12001 caaaactcta ttaaagagtg atgaaggcaa actatcctat ttatcgtccc ttgaccaaaa
    12061 gaaagtttcg tgtcagtgat ccctatatct atcctcataa atttgcagtt ttggatccag
    12121 atggctattt tttaagattt agcgagtaga ataataaaaa ttgggaaaag tctttaaagt
    12181 tagaaaaagg catattgtca ggtgtctaaa agcttgatag tatgcctttt atgatggaaa
    12241 aatcttctaa tactcttcga aaatctcttc aagccacgtc aacgtcgcct taccgtaggt
    12301 atatgatact ggctctgtca attctatcca caacctcaaa acagtgtttt gaggttgtgg
    12361 atagcttcct agtttgctct ttgattttca tttagtataa ctgttagctt acaatggagt
    12421 ttttgttcac cctctcttct ttttgttcgt tttttaaacc aaaacactaa caaaatagtt
    12481 gatttctaaa gttatttagt gtaaaataaa aacattggct caagcctatt taaaattgaa
    12541 tatcgtttta cttgtttatg tcgtgaattg gcacgacgtt tctacaaggt gccggaacac
    12601 ctaacaataa gtaagtcagc agtgagatat ggccgttttt gccatgtcta tttaggggac
    12661 ttacttagag attaagtagg tcttttttta atatttccct taattagtgc ggacgggagg
    12721 tgaaattatc ctgtgaataa tttcaggaat ccagggaatt tcatccctaa tttttgagcc
    12781 cctagctctt tcatttctag gcttaggcta aaatagttcc ccgaactatt ttactctcaa
    12841 aaatcccgtc atagacaata acaagattat tgtctatgat accactacaa cgggaaatat
    12901 ttgataagac tcgcttcgct cgtaatagtt ttatcaaata tatagtatta gcaaaaggga
    12961 tcactataga aatatttaaa caacaagtaa attaactttt agactttagg aggtcttatg
    13021 aaattattag aagagcgcat cctcaaggat gggcatatct tgggtgataa catcctcaag
    13081 gtagattcct ttttaaccca ccaagttgac tttagcttga tgcgagagat tggtaaggtt
    13141 tttgcggaaa aatttgctac tactggcatt accaaggtcg taaccattga agcgtcgggt
    13201 attgccccag ccgtttttac agctgaagcc ttaaacgttc ccatgatttt cgccaaaaaa
    13261 gctaagaaca tcaccatgaa cgaaggcatc ttaactgctc aagtctactc ctttaccaag
    13321 caggtgacca gcaccgtttc tatcgctgaa aaattcctct caccagagga caaggttttg
    13381 attatcgacg atttccttgc taatggccaa gctgctaaag gcttgattca aatcatcgaa
    13441 caggccggtg ccacagtcca agctatcggt atcgtgattg agaaatcctt ccaagatggt
    13501 cgtgatttgc ttgaaaaagc aggctaccct gtcctatcac ttgctcgttt ggattgtttt
    13561 gaaaatggtc aagtcgtatt taaggaggca gatctctaat gcaaactcaa gaaaaacact
    13621 cgcaagcagc cgttcttggc ttgcagcact tactagccat gtactcagga tctatcctgg
    13681 ttcccatcat gattgcgaca gcccttggct attcagctga gcagttgacc tacctgattt
    13741 ctacagatat cttcatgtgt ggggtggcaa ccttcctcca actccaactc aacaaatact
    13801 ttgggattgg actcccagtc gttcttggag ttgcattcca gtcggtcgct cccttgatta
    13861 tgattgggca aagccatggt agtggcgcta tgtttggtgc ccttatcgca tctgggattt
    13921 acgtggttct tgtttcaggc atcttctcaa aagtagccac tctcttccca tctatcgtaa
    13981 caggatctgt tattaccacg attggtttaa ccttgatccc tgtcgctatt ggaaatatgg
    14041 gaaataacgt tccagagcca actggtcaaa gtctcttgct tgcagctatt actgttctga
    14101 ttatcctctt gatcaacatc tttaccaaag gatttatcaa gtctatctct attttgattg
    14161 gtctggttgt tggaactgcc attgctgcta ctatgggctt ggtggacttc tctcctgttg
    14221 cggtagctcc acttgtccat gtcccaactc cactctactt tgggatgcca acctttgaaa
    14281 tctcatctat tgtcatgatg tgtatcatcg caacggtgtc tatggttgag tcaactggtg
    14341 tttatctggc cttgtctgat atcacaaagg atccaatcga cagcacgcgc cttcgcaacg
    14401 gataccgcgc agaaggtttg gccgtacttc tcggaggaat ctttaacacc ttcccttaca
    14461 ccggattttc acaaaacgtt ggtttggtta aattgtcagg catcaaaaaa cgcctgccaa
    14521 tctactacgc agctggtttc ctggttctcc ttggactgct tcctaagttt ggcgcccttg
    14581 cccaaatcat tccaagctcc gtcctcggtg gtgccatgct ggtaatgttt ggttttgtat
    14641 caattcaagg gatgcaaatc ctcgcccgtg ttgactttgc taacaatgaa cacaacttcc
    14701 ttatcgcagc tgtttcaatc gctgcaggtg tcggtctcaa caacagtaat ctctttgtca
    14761 gcatgccgac agccttccaa atgttcttct caaacggaat cgtcgtagcc agcctactcg
    14821 ctattgtcct caatgccgta ttaaatcata aaaagaaata agaaaaagag gtgcgagcct
    14881 cttttttgtt gacaaaataa aaacatctac ctattcatat atgctagtgt catagtcctg
    14941 aaaatcatct gcatcaagaa caatttcaca actcttatac ttttctctta caagtcgttc
    15001 ggcttcatct ggattttcag cctctatact tactaaacgt gataaagttt ctgtaatttc
    15061 tactgtatac tttttcagtt ttagattgct ttccaataat tcacctatta acaataatct
    15121 atgataggag ctatgtagcc actctggcaa tttatctcct acttcccacc catatttttc
    15181 cttcatcaaa gctagttgtt tttcgtcacc aaatttcata ttttcagata accatctcac
    15241 acaatcggga tctgataagg tacagacgat taaacttagg ttatcgtaac ggctttcagt
    15301 ttcataatca ggccagatga cttttaagaa atcatacacc tcttctgatg caacaagttg
    15361 tttcgttttc ataatttccg atactttcct ctacctttaa attctattat ttctttgtct
    15421 cttaatattt gaagctgttg cctaatcttt tctttgatat gattgttctt aacaaagata
    15481 ttttttaggt cactttcaaa acgatacata tcttcaaggg taaattctga accctctatc
    15541 ttatctatac aatttagaat ttctattgtc caacctcttg atgacagaga gctcttcctt
    15601 aaaaataaac cttgcttaaa ttcttttgta actttttctg gatctctaac ttgtccatct
    15661 tgcacaagaa atatccttcc tttagaaggt acttgtgata aatcaatgtt acaaccaatc
    15721 caacctgctc gtctagcagt tggtgcaagt ggttttcttt gaataatcga tttcggtgta
    15781 acaaattgct tcggaaggac aagaaagtta tttacctcaa aattttttgt gtaagttaaa
    15841 aaaaagaaat taggattatt atctgcctgc acacgcttca tcatcgttgc ataagcacca
    15901 tcattgattg ttgatgaaaa atttcctttt ttgctcttta gttcaaactc ctcactacaa
    15961 tgattacagt aaaaatctgc tacaggccga ttattttcaa aatgatttaa gggattgttc
    16021 ccacaattag ggcaataaga ctgacgataa acccaatcct ccgttaagat acgtgccttt
    16081 tggctattac ttttataggt ttctactaat tctaaattaa aatgtaattc catttaaaaa
    16141 ttcctcacta atatgagaac tcactcgata taagcaatta aaatctacta taactagtat
    16201 accacatata tgtacctatt acatttgtat ttcatcactc tcttttatat ttccttctaa
    16261 catatctcat aaattaaaaa tgagtgagat aaatttatcc cactcacctt tctttatatc
    16321 cttacccgtc tttcctcaga cctttggcct gtgacaaaca tggtaaaggt tgctttgcag
    16381 acatttctgc cctcttgatt ggtgatatcc acatccatta cacaagttgt acgaccttga
    16441 tggacacatt ctcctttaat ggtcaataca tcgtcgagtt ttcctgcctt aaggtagttg
    16501 atagaggatt ggagtgtcac cccatcaagt cccagcgaga taaccaccaa accactgatt
    16561 tggtcacata gcgtgaatag atagccacca tgggcattgc catagtagtt gagcgacgag
    16621 ttcactactt tggtcgtcac cacaacgtga ccatctctca ttttttcaat ttcgtaattt
    16681 tcaaaggcag atatagcgtc aaaatgaaag tctttcatcg tttcctcctg atatttcaaa
    16741 cgacactatg atactacttt ttataggact gtgcaaggag aaagctccta gtctagtaaa
    16801 attccgacct gttccacaaa gcgcataaag gctgtctgcc cttgttctgt ctgcttgtag
    16861 actcctgcat cctcaagtac acgcgcaaag atagcaccca cagagtcctt gacgattgca
    16921 agggcttttt ctttatccgt taggtctgga tgttgggatt tgagttggtc tgcccactcc
    16981 tgatgataat cggcaactgt aacagcttct cctacaagat agctagcgac ttgctccact
    17041 tcttctttca gacgtggtgg caagattgcc aagcccatga cctcaatcaa gccgatattt
    17101 tccttcttga tatgttggac atccttgtgg ggatgataga taccatcagg atactctgct
    17161 gaagtctgat tgtctcgcaa gaccaagtcc aactcaaact gtccatcgcg tttgcgggca
    17221 atgggtgtga tagtgtgatg cggtgtcctg tctgtctctg ccaaaatctg cactgcagga
    17281 tctgaatact ggcgccattc ctgcaaaatc ttatcagcca aattgatcaa atcctctttg
    17341 gaatccgaag tcaaacgtag gacagacatg ggccacttga caattccagc cttgacctgc
    17401 tcaaaaccag caaatcggaa ggccttttgc aagggagcca attccatagg aaatacgtga
    17461 cggcctccct gataatgatc atgagttaga atagagcccc ccacaatcgg caggtcggca
    17521 ttagatccag caaaatatcc tggaaactgg tctacgatag ccaacagacg ttcaaaactc
    17581 tgacgactaa tggccatggg acgatgctgg ccatctaaaa agatacaatg ctcattaaag
    17641 taagcatagg gcgaatactg gaaaccccat tcctgaccaa ccatttcaaa acggataata
    17701 cggtgattgc tacgagctgg gtggttaact cgaccatggt agccctcatt ctctagacaa
    17761 agctgacact gaggataatt actagcttgc accaacttgg ctgccacaat ctctttggga
    17821 tctttttcag gcttagagag attgatggta atttcaagtt ctccgtagtc agatggaaca
    17881 cgataagcga tatttctagc aatggccttg agtttgatgt agtcattttt ctgactgagt
    17941 tggtaaaaat cctctatcgc ttgttctgga gagtgggcgt aggttgccca aaaatcacga
    18001 ttgacctgac ttggacaagg agtcaccaaa tccatcagtt cagcaccaag gatttcacgc
    18061 gcagtctgac tatcctcaat cgtctctaat cgaacggctt cttcaaccag ctggtccttg
    18121 aggtcaatca atttatccag attggtctca acttccaaaa caccttctcc cactcgtgcc
    18181 aaaacacgat tggtcagata gattcgatcc atttcctcaa atgagctttc agaaatgaca
    18241 tgtgttacaa atttatttac taaggtcact atagagcctc cttttgacta gtcaaggacg
    18301 cgagtgccac ctgcaacttc agcgatatag aagcttggag cgtatccaac tacttcctcg
    18361 tagtgtttgc ctacagcttc cttaaaggcc tcaacagtat ctttttgaac caaggcaatg
    18421 gcacagccac caaaaccagc ccctgtcata cgagcaccga gaactccttc ttgtgcccaa
    18481 gctgtgtgaa caagggtatc caattccaaa ccagttactt cataatcatg ctccagagaa
    18541 acgtgtgacg cattcatcaa gcgtccaaat gtttccaaat ctcctgcttg gagtgctact
    18601 tgagctttga gggtacgttg gttttcaagc acagcatggc gagcacgttt caaacgattt
    18661 tcatctttaa tcagatagct atattggtca acggcccact cgtccaattc acccagagtc
    18721 tgaatatcca aggaaacttg caattcttcc actgcttttt cacactcagc acgacgttca
    18781 ttgtatttag agtccgccaa ttcacggcgt ttgttggtgt tcatgataac aacgacattg
    18841 tccttcaaat caagtggcac caagtcgtat tctaaagtat tagtatctag gtaaatagca
    18901 cgttggtctg cccccatacc aatagcaaac tggtccataa tgccagagtt tactccgata
    18961 aagttgtttt ctgtttgttt gccgatttta accaaatcga gacgctctaa ttttaaatca
    19021 aagagatgct cagccacgac tcctgtcaag agttccaagg atgcagaaga agacaagcca
    19081 gcaccatttg gaatatttcc ataaacataa aaatcaaaac ctttgtcaat cacgtgccca
    19141 gcttcttgca agaaatgaag gacacctttt ggataattgg tccagttgtg ctctttttca
    19201 aacttgaggt cagcgagagg cacttcgata atgcccttgt cctcaaagtt agctgagtag
    19261 aaacgcaaga cttggtcgtc acgcttacga gctgcaccgt aagttcccaa ggaaatagca
    19321 gcaggaaaaa cgtgcccacc gttgtagtct gtgtgttcac caatcaaatt aatgcggcct
    19381 ggtgaaaaga aggtttgatc tgcttcttga ccaaaaacag caagaaagtc tttgcgaagg
    19441 gcttcagtag taagatgttg tgccatatga attctccttt gactgtccgt taagccatct
    19501 taacgtgtaa tcgttttctt tctattatat ccaaggaatt ttccaaggtc aatccttttt
    19561 actaaaattt tagtaaaata tcagtaaaaa gaagaaaaac atgatatact aacctaatca
    19621 aggaggattt atggctacct taaaagacat tgcacagcta gcctctgtct ctatcgcgac
    19681 cgtatcccgc gtcctcaacc gcgaccagag cctatctgtt acagaagaaa ccagacaccg
    19741 tattttaacc gttgctgaag agctgggcta caccaagcac ctcaagacag gcgagtccca
    19801 caaacccaag caaaagattg ccattatcca atgggtcagc gaacaagggg agctggacga
    19861 cctctactac taccagattc gcctaggaat agaaaaaaga gcccaagagt tggactatga
    19921 tatcttgcgc tattttaatg accacccttt taccctaagc gaggaagtga ttgggattct
    19981 ctgcatcgga aagtttagtc gagctcagat ttctgccttt gaagaatacc aaaagcctct
    20041 tgtatttcta gacagcgata cactttccct gggacatacc tgtattatca cggattttta
    20101 cactgctatg aaacaggttg tcgattattt cctcagtcaa ggaatggacc gtatcgggat
    20161 tctaacaggc cttgaagaaa caacagacca agaagaaatc attcaggaca agcgtctaga
    20221 aaacttcaaa aactatagtc aagcgagggg aatctatcat gatgaactgg tctttcaagg
    20281 aagatttact gcccagtctg gctatgactt aatgaaggag gccattcaga gcttgggaga
    20341 ccaacttccg ccagcatttt tcgcagccag cgatagttta gctatcggtg ccctccgtgc
    20401 cctccaagaa gctggaatca gcctgccaga tcgcgtcagc ctcatttcct ttaacgacac
    20461 tagtctgacc aaacaggtct atcctcccct ctctagtatt acagtttata ctgaagaaat
    20521 gggccgagca ggtatggata ttcttaacaa ggaagtcctc cacggtcgga aaatccctag
    20581 cctgaccatg ctgggaacca gactgacatt aagagaaagt accctaaatc aagaatagga
    20641 taacataaaa aacgaataga gttctaaaac tcctattcgt tttttattcg attacaatca
    20701 tagacttaat tgtcttacgt tcatccatat ctttataggc ttggtcgata tcttccagtt
    20761 tataacttga agtaaagacg cgacctggat tgatatcacc atcaaggacg gcttttagta
    20821 aaaattgctt atcgtatgtt gtagcagaat ctgccccacc tgctacagag atattttgca
    20881 taaatgtcga accaagagca cgattattat agtgtgggac tcctacaaag cccatacgcc
    20941 ctccattatg aagaacacct agcgcctgtt ctatagcagc ctccgtacca acacattcaa
    21001 gtgctgcatc tgctcctcca ccgaggattt cacgcacctt ggtaattcct tcttgaccac
    21061 gttctgcaac aacagctgtc gcacctgact ccatagccat cttttgacgg tcttcatgac
    21121 ggctcataag gataatttgt gatgctccac gcatcttagc cgcgatgaca gcacattgac
    21181 caacagcccc atcaccgata acaacaacct tgtccccttt ttgaacattt gcaacacgcg
    21241 ccgcatgata gcctgtcggc atgacatctg caagagtcaa aagggacttg agcatccctt
    21301 ctgtatagtc agaaggttga ccagggattt taaccagcgc ccagtttgca tagtggaagc
    21361 gaatatattc tgcctgaaaa tcacccccca aattattgcc aatatgattg tcgcaagaac
    21421 cgtcaaatcc agcaagacag gcatcacact caccacatcc atgtgtaaaa gggacaatca
    21481 caaaatcacc tggtttcacc gtcgtaatgg cttccccagc ttcttcaaca atcccaatcg
    21541 cttcgtgtcc actatttttg tgtccagctt tcgtttctgg attacggtac ctccataaat
    21601 ctgaaccaca aacgcacgca cgaaccacac gaataatcac atcatccgct tctattattt
    21661 gcggacgttc aatgctagca agtccaacct gacctgcctt tgtatatact gctgatttca
    21721 tttaaaattt tccttcctta taaagtttaa ttttgagatt taaacgattt aaagctgtct
    21781 gtagctgaga caagcgctcc tctaattttt gcttttcctc ttctaaaata ccaagcctct
    21841 cctctctcgt ttcatctccc ttttggtaga gcgacatata gtcaactaaa ctatctacag
    21901 agacacccgc cgaacgaaaa cacttaataa attccagcgc ttcgatatcc tgatcttgaa
    21961 aatcacgaat cccagtagca gtacgagtaa tcggtggcac aagaccaacc cgttcataat
    22021 accgaatcgt atccgctgaa attcccaaca aatcactggc agatttaata ttcacacgct
    22081 aacctcctct catttcattc actgaattca cgctattcta actcacttat cttattatat
    22141 aactttgagt tgactccaag tcaagcattt tgtatctaat cgaaaaattt ttatcattaa
    22201 caatcatata tctggagttt atacaacgct cttctcttac caatttttga aatatttaaa
    22261 taataaagaa attactctat tccaaataag atagtaccca agtatttttc acttttttct
    22321 aatgttgatg ctcataactc cgttccaagt cacacacctt tcgcttatgg gtttggtgag
    22381 tttctaggtc agcatcaatt tcaatggtaa tattttgaaa accacaatct tttaggaaaa
    22441 ttcgaataga ctctttacaa gtttccatat gttccatttc ttttagacaa acatggacaa
    22501 tggcattttt ttccaaagca tccatagtcc agagattaag ctgattaagg ctggccacat
    22561 tgtccaatcg ctccaggcca ctctttactt gcttgatatc aagaccttct ggcacagcat
    22621 ccaagaaaat cttgagtgta gaccaaaaac gtggaagggc ttttgaaaga ataaagaaag
    22681 aaatgacaag ggacaaaaga ggatctagga tataccagtc cgtaaatcga agaacaatcg
    22741 ccatcaggat aacagctacc caccctagcg tatcttccag aaaatgcaga ctcagaatag
    22801 actcattctt tgtctttccc ttaccaacca ccagactcgc taacagattg atagtaatcg
    22861 caataattcc taaccagaga atcccctcat cattgactgg ttgcggatgc aaaatcttcg
    22921 tgacattttc caaaatgact agaacagagc ccgttacgag aatcacagct gttaccaagg
    22981 ctcctagcag gctaaaccgc ttatagccca aggtgtactg attgtcttct tcacgattgg
    23041 agattgtttc tagaaaagct gatattccaa ttgcaatcgc atctcccaag tcatgcacag
    23101 agtcagcaag aacagcgcta gaaccaaata ctccacctgc aataaactca acaatggcat
    23161 aagtcaaatt taagaaaaaa gccacccaaa cagcatattt tgccttcata tttctcattc
    23221 ctttgttata atagatttat gaacaccttg ttcattatca ttatccacta aaactcaaag
    23281 aaaggataga agcaaaacgt cagctttatt cagttctgaa caatttgcct taagtgtcca
    23341 tatgactaac attgaccgcc gtatcagcaa aaccaaaaaa gccatctatc aagcttttat
    23401 acaacttttg aatgctaagg gctacgaggc cactactgtt caggatatca ttgatctcgc
    23461 agatgtggga cgatccacct tttactgtca ctatgagagt aaggagcttc ttctggacca
    23521 gctctgccgc tacctcttcc atcatctttt tgaaagagag caagccattt caaccgagga
    23581 ctacctcgcc cacctctttc tccattttca taaaaaccaa gaccatatca ccagtctgct
    23641 attttccaaa aatgactact tcctccgtca actccataaa gagctagaac accatgtcta
    23701 ttccgtgcta gctgataatt taaaagaagc ccaccctaat ctgcctactt cttacctcca
    23761 acacttggtc atgtccaact ttatcgagac attgacctgg tggctcaaaa aaggtcaaga
    23821 tttcacagac caggaagttg tccaatttta tctagacctt ctcattccta aaaattgaat
    23881 atagagtaaa gcttcagttg tcttatttct aggttactga gttttttatc ttttcaacaa
    23941 caaaagagga cccgccgatc ctctttttca tactataaat ccttgattat caactatatc
    24001 tgttttaatc gaaatctcaa aacagcactt tcaaacatct tttcctagtt aagtaaatca
    24061 gtattctgct tagctgcctt gctccattga taccaaccaa ctagactgtt aatgagataa
    24121 attagatatt tcccttgaat ttgcaggctt tctccccacc agagatagat tgaaaagaca
    24181 ttggtagccg cccagaatat ccactgttca cggtaaacag ctgtcatgag gatttgccct
    24241 accccattgg ttgcatctgt gattgaatca cgatagggac gattggcacc aatagactga
    24301 taaatgaagc caaaggccaa ccaccaaagc acactaatgg aaagatactt tgtccagccc
    24361 ttgccgtcca gtttacgcgc gacaaactcc tgcttttcct tcttaaactg tgcctgataa
    24421 atccaaacta gaagtccaat tggctgcatg actgtgaagt aaagtgtcgt cagcacctca
    24481 ccataaaagc ctttctgtag ggccaaaata aggtaaataa cagagttaat caagccaaaa
    24541 agataattac ttgctcgacc ttccgataca aagattacac agataatccc tgtcaagcta
    24601 caaatcatcc caatccagtc aacaatacga tgttcgtaaa ccaactccag ccagagagga
    24661 aaacttccta aaaccagcaa ataaatccac tgggcaaaac tacgatgggc aaagaggtca
    24721 tcccagatag ccttcatagt tcctgaaaat cctaaatcag ccatagccgc aaccatacga
    24781 cggtaaccac ctgacatttc acctagggtt gttttgatat tttcaatttt cttttgcaaa
    24841 taagtatgca tcatttctcc ttttgttttt aaagagccgt gtctggatag actttcggac
    24901 gcaacgctct attagataat gaactgccta tacacaagat ttctaacctt agtcgacatg
    24961 agctgaaacc tcttatttgt taagtagttc acaaaatatt atacacctat tttatgaata
    25021 gtcaactgtc tttacagtaa aattttagaa aatcatgaaa attttctctt tctttccatt
    25081 ttaagtgaca ttcagtcatt ctcacatcaa aaaagcccag acgaaattgt ctgagcattc
    25141 ttttatctag tcgtttaagg aagttgagtt cagtatgttt aaagtctctg tcccatcatt
    25201 tcttcaacaa accttgttct tggagaaact ccttggctac ttgctttgct gacttgcctt
    25261 caacaccgac ttggtagttg agctggctca tctggctttc tgtaatctta ccagccaatg
    25321 tattaagaac tctttccaac tctgggtgtt tcttgagaag agcttctttc atgagtggag
    25381 ccccttgata aggtgggaag agttgcttgt catcttccaa gacctgtaaa tcataacgct
    25441 ccaattccgc atcagtcgaa taggcatccg tgatttgaat atcccctgac tgaatagcct
    25501 gatagcgaag ggctggctca atggtcgcta cattgagatt gagaccatac attgattgca
    25561 agcccttatt tccatcttca cggtcgttaa actcgagtgt aaaacctgcc ttcaactgcc
    25621 cttccacttt tttcaagtct gaaatggtct tcaagccata ttcttgagca atctttttcg
    25681 gaacagctac agcataggtg ttttgataag acatgggttt gagataggct agatgatcct
    25741 gcttagcaat gccatcacgc gccacctgat aaacctgttc tggttcatga ctcaccttgg
    25801 gtgatggttg aagcaaactt tcagtcaccg taccagtaaa ttcaggatag atgtcaatat
    25861 cgcctttttt cagagcttca taaaggaagc ttgtcttccc aaaattcggt ttaacagtcg
    25921 cagtcatgct ggtattttct tcaatcagca acttatacat attggccaaa atttctggtt
    25981 ctggacctat tttcccagca ataaccaagt tttccttctc tttttgaacc aaaagagctg
    26041 gactataaga cagacccagt aataaagcca ccaaggcaaa acctgagaaa atcgtccgta
    26101 attttgcttt ttccatcact tttagtagga agttaaaggc aatggctagc actgcagaag
    26161 aaagtgcccc aatcaaaatc aaactggcat tattacggtc aattcccaaa agaataaagg
    26221 aacctagtcc ccctgcacca atcaaggccg ccaaggttgc cgtaccgata atcaaaacag
    26281 ctgccgtccg aatcccagac atgataacag gcatggcgag tggaatttca aatttcttga
    26341 gacgttccca tctggtcatc ccaaaggcaa tcccagcctc ttgcaggttc ggatcaattc
    26401 ccttcagccc agtgatagta ttttgcaaaa tagggaaaat cgcataaatc actagagctg
    26461 tcaaagccgg caaggtccca attcccatca aagggataaa gagccccaac aaggccagag
    26521 acgggatggt ctggaaaata cctgcaatct gcaagaccca gtcggccagc ttctcatgat
    26581 agcgaagaaa aacagccaag ggaatcgcaa gcaaaatagc tagtaacaag gtcaaaagcg
    26641 acaactgcaa atgttgagat agagctgtca accaatcact aaaacgatcc tgaaaagttg
    26701 caattaaatt agtcatgaac actacctcca aacaagtctg ctacaaagtc tgttgcaggc
    26761 gcttttaaaa ttgtctcggg attcgctacc tggcgaattt ctccatcctg caagacagca
    26821 atacggtccg ccaacttcaa ggcttcatcc gtatcatggg ttacaaaaat cgttgtcatc
    26881 ccaaactctt tatgcaattc ttttgtcaga acctgcaact gttttctcga aatagcatcc
    26941 aaggccgaaa agggttcatc catgaggaaa atcttgggct gaccaatcat agctcggaca
    27001 ataccgaccc gttgctgttc tccaccagat aattcactag gtaagcgatg cccatactcg
    27061 gctactggta aaccaacctt agccaaaagc tcttctgttt tcttcgtaat ttcttccttg
    27121 ctccacccct tcatttcagg aatgagagca atattttccg caactgttag atttggaaaa
    27181 agagcaatag cctgtaaaac ataaccagta gaaagacgaa gttcacgctc atcatagtct
    27241 ttgatgcgct tcccatccat ataaatattt ccatcagttg gttccaaaag acggttaatc
    27301 atcttgagca tggtcgtctt acctgaccca gaaggcccta ctaaaaccat aaattcccca
    27361 tcctcaatct gtaagttgac atctctcaag acatcctttt ctgtgtagcg cagtgctaca
    27421 tttttgtatt caatcattct ttgtcctcaa tttaaaactt ccctcgattg gtcaagtctt
    27481 ctaccttagg cataacttcc ttattatccc aatgctccac aattttcccg ttctctaaac
    27541 ggaagatatc gtactgggca taagcaacgc catcaatctg agtctgacca tagctaacca
    27601 catagtttcc ttgtcctaag agttggaaaa caaagtcaaa agtgacacta tattcagcca
    27661 catagttttt ataagcagca cttccttgtc caatatcatg attatgctga atcaaatcgt
    27721 ctgccacata atcactccac tgctctagct ccccattttg gaaaatttct gtcaagaaac
    27781 ggcgaaccag ctttttattt tctgctttct tatccaaatc cttgatttca aaatctccaa
    27841 aaatttgatc tagttggtca ttttcaggtg ttcgatagta gtcaatgaca tcccaatgct
    27901 caacaataca accattctca tcctcacgga aagtatccgt cgtcacccat tgagcttctc
    27961 caccattcag atattgatga acatgaacaa agaccagatt gccatcctca atggtgcgga
    28021 caatcttaat ctgacgctct ggatgacgct caaagaaatc tgcaaagaag gctgcaaatc
    28081 cttctttccc gtcaggaaca cctgtcgaat gttggatata ggtatcccct acagactggg
    28141 cttgagcctc agcaactcgt ccgtcttgaa tggcatggat gtataggttg tgagcatttt
    28201 tcacttgttg tgacatattc taaacctcat ttcccttctc tttcagattc gccaaaattc
    28261 tttcttgaaa accttcaaat tggtgaattt cttcctctga aaatcctttg taaaagatag
    28321 tatccaattt ctgactgaca cgatgcccca cttctttctg ggacttgcct aactccgtta
    28381 aaactaaata cttcttacgc ttgtcttttc cacacggact aacaattaca agcttttgtt
    28441 cctctagctt ttttatcata gtcgtcagcg tattattcgc aagtccagtc gcaagcgcga
    28501 tatctgtcgc agttgcgcag ccagtttcac tattccataa aaccgctaaa atcttgccct
    28561 gttcacccct ataaagagcc tcaggatctt gactcagtaa cttttgaaaa atccgcccat
    28621 tcaacaaacg aatatgatgg gctagcaaat gaccatcttt cataacacct ccaatttatt
    28681 tcgatatcga aatgaataaa acaattgtaa cactcatcgt tctaactgtc aactatttcg
    28741 atttagaaat aatttttgat aattatccac accaccatac tccggctcaa ctaactttta
    28801 acgagagttt ctaaactcct tcgtcctcca gtctacaaaa gccttccatt cgtactatcc
    28861 tatattttat gaggggacac atttttccta tcagaccatt tattttaaag atagaagtaa
    28921 atcataattg cttccatctg ttcttttata gtatattgaa gttagactag agcactgtat
    28981 cttctaaaac attgatagaa agcgatttga atttcccaat caatttgttc gtatttatag
    29041 catttcgaaa ctggaatagg acaccatgac tgctaaaaga tttctataaa ttcatttaat
    29101 ttcctcaatc aatttgttca tatcttattt cattccgcta taatttcacc ttaccctatc
    29161 tttttcgtag cacccttcaa acagcctatc ccctaccgtt tgacgattcc tcacttcgct
    29221 ccacttccat tacagaagtt tcttcactac tatgggctcg gctgacttct catgattcct
    29281 tgttactact atttgaacgc tcacgagata gatcttacaa aaaatgcttt gatccacaat
    29341 ggaatcaaag cattttaaag agttcctcat acataagcgc agaagtcgca gttcctctgt
    29401 acttggcttc ttctcttttg acaaagcgag ccaagttgag caactcaggt gctggatgtt
    29461 tgggatttag gagcaattca cgattgacca ggcctgagag acgaactgcc tgcaattgct
    29521 catttgtagt aggcagtttt ttagtagtct ctaggagagc agcaactaaa tcttcactca
    29581 aatcatgtcg agcatgattg taaagatctt ttataaggct ttctaggttt ggttctacca
    29641 tccctaccac ctcccttatg gtttaataat gtttaatcaa atcaaccgtt gaacgatcca
    29701 atttcttcac caaggcttgt aagaaagctt gcgcttctag gaagtcatcc attgcataga
    29761 gggtttggtg agaatggata taacgagcgc agacaccgat agttgttgat gggacaccac
    29821 catttttcag atgagctgca cctgcatctg ttccgccttt accacagtag tattggtact
    29881 tgataccagc ttcttcagcc gttgtcaaaa ggaaatcctt catccctggg agaagcaagt
    29941 gacctggatc atagaaacga atcaaggttc catctccaat cttgccttga ccaccgtaga
    30001 catcacctgc tggtgagcaa tcaactgcga ggaagacttc tgggtcaaac ttggttgtag
    30061 aggtatgagc gccacgcaga ccaacttctt cttggacgtt agaacccaga tagagttcat
    30121 tgccgagttt ttgacccgat aaagcttcag ctagctcgct taccatgagg acaccgtagc
    30181 ggttatccca agcttttgag atgatatttt tttcattggc tgtcaaaatt gcagaactat
    30241 ctggtacaat ggtatcacca ggacggatgc caaaactttc tgcctcagcc ttgtccgcaa
    30301 aaccaccatc aaaaacgata tcggcaatgg ctggcatggt tggtcccccc tttccacgag
    30361 tcaaatgcgg aggaacagaa cctgaaatca caggaatttc atgaccatca cgagtcaaga
    30421 gtttgaaacg ttggctgcta accaccatgg ggttccagcc accgatttct acgacacgga
    30481 aggtaccatc tggcttgatt tcgctgacca taaaaccaac ttcgtccata tgagaagcga
    30541 ccaagacgcg cggtgcatcc acagcttctg aatgtttgat accaaaaata ccacccaagc
    30601 catctgtcac cacttcatcc acatgcggtg tcaacttttc acgaagataa gcacggacag
    30661 gcgcttcatg acctgagact gcagcaagtt ctgttacttc tttaattttt gaaaataatg
    30721 ttgtcatttc agttccttct ttctttcatc cattttacca ctttttatag gagaaggata
    30781 gtgggaaggt ggatttctaa gttagtatct tagtcctgct ctatcttaga aaaggatagt
    30841 attctcttgc atgtagtgca aaatctagta aacattccaa aattaactcg aatatttatt
    30901 tccaaacaaa aaaacaatac accatcaaag ttgtttggat ttttcatgaa atttacagaa
    30961 aatagttgac ttccctttct tctttcttta aatatatagt tggttgagtt tggaatagta
    31021 cgctgtagct gctaaaacat ttctagaaat taatttgact ttcctaatag agttgttcat
    31081 atcttatttc aatttactat agtacaaaac tagaaaagga aaaaatcatg accaaaacaa
    31141 ttcttgttac aggtgggact agctacattg gctcccatac cgttaaagct cttttaaatg
    31201 ctggctatca ggtgcatgtc ctcgataatc tctccacagg aaatcgagcg gctgtggata
    31261 gtcgtgctag ctttaaagaa ctggatgttt atgatgctag cgccttaaag gcttacttag
    31321 aagaaaatca gattgatgct gtcctccatt gtgcaggtga aattgttgtg agcgaaagta
    31381 ttgaaaatcc aagtaaatac ttcactgcca acgttgctgg tatgaaccaa gttctcaaag
    31441 tcttatctga agttggcatt caaaaaatca tgttctcttc gactgcttct ctctatggaa
    31501 ataactgtat tgacaagccg gtgactgaag ataccctgct cgaccctgtc aacccttatg
    31561 cagagacaaa actgatgggc gaacgaatga tttactggat ggccaatcgc tacgactgga
    31621 aatatgttat tttccgttac tttaatgttg ctggggctga aatggatgct tcaaacggtc
    31681 tgcgtgtgaa aaatccaact cacatcattc caaatatcaa caagaccgca ttgggacaaa
    31741 atgatagctt aaaaatattt ggagatgact acgatacacg tgacggttca tgtattcgag
    31801 attacattta tgtcttggac cttgcatagg ctcatgttaa aggaatgaac tacctatttc
    31861 aagaagacag ttcttctcaa atctttaact taggaactaa aaaaggctat actatcaaag
    31921 aaatctttaa aactgctgaa gaattactaa attaaaaaat cccacacgag attgttgctc
    31981 gacgtgctag tgacccagcc agtgtcctag cagatgcttc aaaagctaaa caatatcttg
    32041 attggacggc tagctactcc ctcgaagata ttattttatc agattaaggt aaaaacattt
    32101 tagaatagga taacaaagga gaaagtcttg ttgctctctc cttgttgtat ttattaaatt
    32161 gtcagaaatt ttattgacag attagaaaaa taatgttaca tttatatccg cagatatctt
    32221 tcgataccaa atctacatga aaggacgggg tatgaaactt tctcattatt taattggctt
    32281 acttctactc ctagtctttc tctctattag cattgggacc agtgattttt catggggaaa
    32341 gctatttgat ttcgaccagc agacctggct cctctttcaa gagtcccgtc tcccaagaac
    32401 tatcagtatt ctcctgactg cctctagtat gagtatggca ggccttctca tgcagactat
    32461 tacccaaaat cagtttgctg caccgagtac agttggaacg actgaagccg ccaaactggg
    32521 aatggtgctg agcctttttg tctttccatc ggctagtctg acccacaaga tgctcttcgc
    32581 ttttgtttca tccatcgtat tcaccctctt cttcctagcc tttatgacca tttttactgt
    32641 aaaggaaagg tggatgttgc ctctgattgg gatcatctat agcgggatta tcggctcagt
    32701 cacagaagtt atcgcctatc gtttcaatct ggttcagagt atgactgcct ggacccaggg
    32761 ctccttctcc atgattcaga cccatcagta tgagtggctc ttcttaggcc tcatcatcct
    32821 gataaccgtt tggaaattat cccaaacctt caccatcatg aatctaggga aagaaaccag
    32881 cgagagtttg gggatttcct actccctact tgaaaaactg gccctctttc tggtggcgct
    32941 aacgacaagc gtcaccatga ttaccgtggg tggcctacca tttctcggag ttatcgttcc
    33001 caatcttgtt cgcaagcgct atggagataa tctaagtcaa accaaactca tggtcgcact
    33061 ggttggtgcc aatctagttc tggcttgcga tatcctatcc cgagttctga ttaggcccta
    33121 tgagttgtct gtcagtctct tgctaggaat catcggtagt ctcgtcttta tcctacttct
    33181 ctggagaggg ggacgaaaag atgcagacta aaagcaaaca taccaagctc ttctggattc
    33241 tcattattct tgccatcgga gcttgtcttc tctacttttg gcccatcact cacttgtcag
    33301 cctttgcttg gaagttgcgt tcccaaaaga tcatcgttta tctcttggta gccatcgcga
    33361 ctgggatttc gaccattagt tttcaaaccc tgacggaaaa tcgcttcctg acgcctagta
    33421 ttttgggaat tgaatccttc tacgtcctac tacaaaccct actactggtt tttgaaagca
    33481 agtttcttca acttggcaaa tcccctatct tagaattcct agtcttactt cttgtccagt
    33541 ccctcttctt tctcgcctta caaggttact tgaagacact gatgaagcaa gacctggtct
    33601 tcgtcctgct gatctgtcta gcgctcagaa gtctctttcg aaatatcagc accttccttc
    33661 aagtcctaat ggatccaaac gaatacgata aactgcaaaa tagtcttttt gcctcctttc
    33721 aacatctcaa cacttccatc ctagccatcg gttctctgat catcctcgct ttgacaatct
    33781 ttttctttcg aaaagcagtc gttctagatg tcttgcacct gcaaagagaa acggctcaga
    33841 tattgggact cgatgttgaa aaagaacaga aagagctcct ctggggaatc gtgcttttga
    33901 cctcaacggc cactgccttg gtaggaccta tggccttctt cggctttata ctggccaacc
    33961 tcacctacct gattgtcaaa gactatcagc acaagttact ctttatagtg gccattctgg
    34021 ttggatttat tagcttaacc ttggggcaag ccttgattga acgagtcttt gcactggaaa
    34081 ttcgtatcag tatgatcatt gagagtgtgg gtggcttctt attctttatc ttactatata
    34141 ggaggtctcg tcagtgaaac tggaaaacat tgacaaatcc attcaaaaac aggatatttt
    34201 gcaaggcatt tcgcttgaag tcagtcctca aaaactgaca gcctttattg gtccaaatgg
    34261 tgctggaaaa tcgactctcc tctccatcat gagcaggcta accaagaaag atcagggagt
    34321 tctcagtatc aaaggacgtg aaatcgagag ctggaattcg caagaactgg cccaagaact
    34381 aaccatccta aaacagaaaa tcaattacca agccaaattg actgttgaag aactggtcag
    34441 ttttggacgt tttccctaca gccgaggtcg acttagatca gaagactggg aaaaaatccg
    34501 agaaactctg aactatttag aactgaccaa cttaaaagac cgctacatca atagcctgtc
    34561 aggggggcaa ctccagcgcg tctttatcgc tatggtactg gcccaggata cggactttat
    34621 cttgctggac gaaccactca acaatctcga tatcaagcaa agcgtcagca tgatgcagat
    34681 tcttcgacga ctggtggagg aactcggcaa gaccattatc atcgtcctcc acgatatcaa
    34741 catggccagt cagtatgcag atgaaattgt cgccttcaag gacggccagg tctttagcaa
    34801 gggaagaacc gatcaaatca tgcaggctga cctgctcagt caactttatg agattcccat
    34861 cacgctagct gatatcaatg acaaaaagat ctgtatctat agctagtaac ataaaagctc
    34921 aagttagaga accttcagtc tcttagtcaa taagattaag agactcccta aatcgttatc
    34981 acattttaaa aaggagaaat tatgaaaaca tcccttaaac tttatttcac tgccctagtg
    35041 gccagcttct tgctcctact tggtgcatgt agtacaaact caagcactag tcagacagag
    35101 accagtagct ctgctccaac agaggtaacc attaaaagtt cactggacga ggtcaaactt
    35161 tccaaagttc ctgaaaagat tgtgaccttt gacctcggcg ctgcggatac tattcgcgct
    35221 ttaggatttg aaaaaaatat cgtcggaatg cctacaaaaa ctgttccgac ttatctaaaa
    35281 gacctagtgg gaactgtcaa aaatgttggt tctatgaaag aacctgattt agaagctatc
    35341 gccgcccttg agcctgattt gattatcgct tcgccacgta cacaaaaatt cgtagacaaa
    35401 ttcaaagaaa tcgccccaac cgttctcttc caagcaagca aggacgacta ctggacttct
    35461 accaaggcta atatcgaatc cttagcaagt gccttcggcg aaactggtac acagaaagcc
    35521 aaggaagaat tgaccaagct agacaagagc atccaagaag tcgctactaa aaatgaaagc
    35581 tctgacaaaa aagcccttgc gatcctcctt aatgaaggaa aaatggcagc ctttggtgcc
    35641 aaatctcgtt tctctttctt gtaccaaacc ttgaaattca aaccaactga tacaaaattt
    35701 gaagactcac gccacggaca agaagtcagc tttgaaagtg tcaaagaaat caaccctgac
    35761 atcctctttg tcatcaaccg tacccttgcc atcggtgggg acaactctag caacgacggt
    35821 gtcctagaaa atgcccttat cgctgaaaca cctgctgcta aaaatggtaa gattatccaa
    35881 ctaacaccag acctctggta tctaagcgga ggcggacttg aatcaacaaa actcatgatt
    35941 gaagacatac aaaaagcttt gaaataagcc acttcaaact caatttaata ctcaatgaaa
    36001 atcaaagagc aaactaggaa actagccgca ggttgctcaa aacactgttt tgaggttgca
    36061 gatgaaagct gacgcggttt gaagagattt tcgaagagta taaaattttg acattctaaa
    36121 acgcatccta tcaagttcac tcaaacctga taggatgcgt ttttatcatt ttgaaatcta
    36181 tttaccccac ctcattcccc ttcttgattt cgtttaaggg aaaagcggtc tggaacgggg
    36241 tccttacctg ttttcgacca gggatgacaa cgtaaaatcc gagccaagcc catcaatacc
    36301 cccttaaacc catgtttttc aatagcctga atcatgtagt tggaacaagt cagctcaaag
    36361 cgacaagagg gtggaaagac tggtgagata aaacgttggt aaaagcgcac aggcgctatt
    36421 aaaattcgtt tcattatttc ttagttacag ccatggtgtg tagttggctg atttcttttt
    36481 tattaagacg acgggattct cctggacgga gtcctgtcaa gtctaggtgt ccgaaacgag
    36541 tccgagacaa cttatctact tggagaccaa cagcttcaaa catcttttta acctgatggt
    36601 tacgcccttc atggatggtc aactgcacca cagagcgatt tttgactggg tccactttga
    36661 gaatttcata aacagctggc ttggttttct taccatcaat ctcaagacca cgggtcaagg
    36721 ggcggagatt gtccttattg gccacacctt taacacgcgc gacataaacc ttgtcaatct
    36781 cattacgagg gtgaatcatc tcgtctgtaa agtccccatc attggtcaaa atcaagacac
    36841 ctgatgtatc ccagtccaaa cgacccacag ggtaaatacg ctctttgaca ttgggcaaga
    36901 ggtcgacaac cgtcttgcga cccttatcat ctgtcacact ggaaatcaca ccgcgtggtt
    36961 tgttaagcag atagtagacc ttttcttcgt tgtagatagg ttgaccttca acttcgacct
    37021 tgtcgcctga cttgatagtg gttgctagtt cacgcaccac ttggccgtta accgtcacca
    37081 agccttgctt aatcagctct tctgcttttc tcctactggc cacacctgcg tgggcaatat
    37141 acttattgat tctcatcttc ttctatcctt tcaccaaata attggctttc ttgggcttga
    37201 atctcaagct catcaatcac tggtaattct tctaaatggt ttatccccat gtaatctagg
    37261 aaataatccg tagtcacata gaggttgggg cgccccaata cttccttttt cccgtcttcc
    37321 tttatcaggt caaaagcctg caactttgcc aaggctccac tcgagttaac tccacggatg
    37381 gcatcaattt ctatccgcgt aatcggctgt ttgtaggcaa taatggacaa ggtctcaagg
    37441 gcagcccgag acaagctctg gttgataggc gccttagagt attccttcaa aatctctgca
    37501 aattgaggct tggtcaccaa tctataagca ccacttgtct caatcaaagc caaactggaa
    37561 tctgggtcct tttcatactt ctgggctaat tttcctaaac tttgctggat gcctgtcggt
    37621 ggcagagaga ggagttcagc taactggcgg acccgaatcc catcttcacc cgctacaaac
    37681 aagagcgctt ctatttttgc taaagtactc atctttcctc tctatcaagt ctagctttga
    37741 ggcacttgac tttcttcctt cttttccatg agatagatat ctccaaaact ctcctcttgc
    37801 acgaggatca actcctgggt tttgattaac tctagggttg ccaaaaagag ggtgatgacc
    37861 tcttggacat tctgggcttc cttgaacaaa tcctgcaagc gcaattgatc tcgtccaatc
    37921 aaggactctt tcacgataat catcatgtcc tcaatcttat actcatcccg caagatcgtc
    37981 gtgtgatttt gtgcaaactc ctcttttttc ttggctagga tatttgaaaa agtcaaaaag
    38041 aggtcaatgg tcgtcttgtc atgcacaagc tccgcatctt cgtaaatcaa ctctgtcggc
    38101 gctttggaat aatactgggc ccgttcttgg tgcttggctt ccaagtgctc acccaagagc
    38161 ttgaacttgc gatattcttc gatttgagag aggaggtcct gctccaggtc atcccccaag
    38221 tctgtcactt ctgctacctt cggaaggagt ttacgactct taatcagcat gagctgacta
    38281 gccatgacca tgtactcacc cgtcacttcc agacgcatgg cctgcagggt tgagacatag
    38341 gctagatact gttcgatgac ttccgtaatg ggcacatcgt agatatccat ctggtactta
    38401 gaaaccagat gcaagagcaa gtccaggggt ccttcaaaat cttttaattt aatatccatt
    38461 atctatattt ttctaaggtc aggactgttt ttaatcctaa tttttttgca atttcgtaca
    38521 aatcgacctt gttttctatt tgtcttagaa taaactgttc acgtaagact tgagctgata
    38581 aggatggaaa acctttctcc ttgacaaaag attctaactg acgaaaggcc cactgacgag
    38641 aatagggttt ctctcctctt tcaaaaagat aggtctgccc catcaagggt tccaattctg
    38701 aaagcaaggc cgtgggaatg gtgacaatcc tctgttggga agccttgctg attcgcaaca
    38761 cctgaaaatc cagattgatg tccgcaacct tgatggctaa aatctcactg ggcaagagcc
    38821 ccatttctag gattaagagc gctagcaagc ggccctctgg atggtcgctt tcctgccaaa
    38881 aagagtctag gtatagaatc tctggctttt ccgtcttctt ttcagcttgt ttggctaatt
    38941 ccaagcggta aaagctgtcc acctctcctt tttgatagag aaagtataga aattggttac
    39001 aggccgaaat ctttcgcttc tgggcgctga tttttagatt ggctagctgg gcttggtaaa
    39061 tcttgagact ggtctcagaa atccgctcac ctaccatgtc taaaaattgc tccaaatcat
    39121 acttatagga ctgcttggaa ttgacagaca agccctgctt ttcctctaaa aaggctgaaa
    39181 tcctgtctct cattggcatc gaatctcata ttccttacta aagtcatgca agagggcatt
    39241 aacggccttg aggatggact tgcgcgtaat aatcccttgg aaaataccct ctgcatccac
    39301 aaccggtaag aaggactcat ctactagctt gtgcaagacc tccgtaatgg tgaaatcagg
    39361 cgaaacaacc gctacgtccg tttttgtcat atgaacgata tccgtatccg ccatgatttc
    39421 ttggctcaag tcatgctcca tctgataagc cataatatct ctgagtccaa tcgtcccaac
    39481 aaactgtttt tcatctgtca caacgggaac acgggtatag gtcatctgac tgagcaagag
    39541 ggtcgcatga tccgcattgt gggtatcaat caacacagct agatttttag caggggtcaa
    39601 aaaagtttcc tcctgcccca acaagaaagt ctcaaactcc ttggcaatca tcggctaaac
    39661 tccttggaca aacctggata cacctcgtga tctcgtgtca aaaagtccac tttgaagtaa
    39721 ctatcatcaa tctccacacg agcatagaga cattctctga tggtacctcg tggttgactg
    39781 atagaacctg gatttagaaa gaggatcttg ccttccaacc aagcacttgg cacatgcaag
    39841 tgaccataga ggcagatagc ggcctcttcc tcctgagccc agtagtccaa cttttgaaag
    39901 ttgaaattga tgtcaaacaa gtgaccatga gtttggataa tcttggtcga accaagctca
    39961 gtcaccagac gttctgggta gccggcgtag aagtccatgt tccctttaac aacgcggatg
    40021 ccctcccaaa gtggagaatc cggacgtagt tcagaatcgc cgttatgaaa aacagcatcg
    40081 actttgccca catagcgatc acggacttct tccacaatca agctatcgcc atgggaatcg
    40141 ctcattacaa tgatggtttg ctttgccatg atggaaatac ctccaaaagt ttcttaacgg
    40201 ctaaggcacg gtgagattga ctattttttt cttccagggt taattcagct gatgactcac
    40261 ctgtttctcc tacaaggaag aggggatcat agccaaagcc attttcaccc ttaggttcaa
    40321 agttaatata acctgaccag tctgcttcaa caactaaact ttccttattt gggctggcta
    40381 cgactagggt tgtgtggaac tgagccgagc ggtccttgag ttcaaagacc atggccaatt
    40441 cgtgcaagag tttggcatta ttttcacggt cagttgctcc cacacctgcg aaacgagctg
    40501 accagacgcc tggtaagcca ccaaggacat cgactttgag accagaatca tctgccaaaa
    40561 ccatcttgcc cgttaattga gaaatggttt ctgccttaag gcgggcattt tcttcaaagg
    40621 tcatacctgt ttctgctact tcaggcaggt cagggtagtc attaagattt tccacatcgt
    40681 agcctaactt atcaaagata gctcggaatt ccttggtttt accttcgtta cgagtcgcaa
    40741 tcaacaaggt ttctctgacc ttgcttgtct caaagaaatc atgaacattg accccttctt
    40801 taggcaattc tacatacaag agttgcccat tttcaaccaa gagcagggcc ccatgacgtt
    40861 gaataacttc caagtttcgt cccatttctt gattaagcat gtctaccaca aaggagaaca
    40921 aacggtagat agaaccatag cgtaaaacag taactgaaag cgggaaacca tactcttcat
    40981 ctccaaatat tttggagaat tcaaacagag gaaaatctgt cttatagtcg ctcaaactgt
    41041 taacgccacc aaaaatacta taagacccaa cataccagtc ctggtcatcc ttatattcat
    41101 aaattttatt tgtcataatt ctacatgctc cacatgaatc tctttttcca gccattcttc
    41161 accaatttgt gcaaaacttt ggctactggc tgttgtgtaa aaacggtgat ggagtggtcc
    41221 agcatcgcga ccacgattga tttcaaaata attgagtaag actgagatat cccgtacgca
    41281 ctctgcccca ctatcgatga gctgaacctt tggccccatc acattttgga taatagggcg
    41341 aaggagtgga taatgagtac agcccaaaat caggctatcc acctttccaa ccaagggacg
    41401 cagggtttca tagaccacct tcttggtaac actggttgac agggcacctg actcaaccaa
    41461 gggagcaaac ttgggacagg ccaagctctc cacctgtaag tcgggatcca gatcatggat
    41521 tttctgacgg tatatgtctg attgtaccgt catgggcgtt ccaatcactc cgattttccc
    41581 accttgactg gacttgatgg ctgccgaagc tcctggcaaa attacaccca agacaggaat
    41641 atctagttga gccttgattt cttcccagac gaccgcagtc gcagtgttac aagcaatgac
    41701 aatcattttg acatccttgg tcaagagaaa gttgaccagc tgccaagtat attcacgaat
    41761 ttgctcagca ggacgggggc catagggcgc ccgcgccgaa tctccaatat agacgatttc
    41821 ttcatgggga agctggcgca tgagctcgcg cacaacggtc aagcccccga cacccgaatc
    41881 caaaaaacca attggtcgat tatccataca gtcttctttc tagtcttttt ttctgattga
    41941 tcgttcatta tgattccttg tactgaacga actgaccgac agcttgatag aatgtcaact
    42001 acctctctct taatcataat caaatatcaa tagaatgcaa ggaaaaagtc tcatccctaa
    42061 accataacca acatagtgag aagtctggaa ctttcatccc agacttttcc ttatttattt
    42121 tttcttttta ttgttagcaa gggctgcctt ttgttggcgg atgatttggt ggtaaacttg
    42181 ttgtacctta gcttcgcttg gtttttgacc atttgcactc aaaagagtac gaactgcttc
    42241 agcattcaaa cgtgggttgt cagcaaattc tttttcgatt tgcttacgaa ccaagtacat
    42301 tcctcctaga gctcctccta gaaaagctag cacaatcaat acaattgcta aaagtaaatc
    42361 catcattttt ctcctgtttc tttttgagtc cctttcatta taccacaaac tagccaccct
    42421 gaccagtttg ttctacactt tcagggatgg aatagacagc aaaaagaacc ctgcttttct
    42481 gaaaaagagg tctccttgct aagtttatac tcaatgaaaa tcaaagaaca aactaggaag
    42541 ctagccacag gttgctcaaa gcaccgcttt gaggttgcag ataaaactga cacggtttga
    42601 agagattttc gaagagtatt aatttacata aatagccagt gtttgatagg gtttgagtag
    42661 aattttctca gacacttctg catcttcata gtttgatatc aaaatctgtc cattttggta
    42721 gactgctggc aagtcgattt ccacttcttt agcataaaag ttattgagca ctagtaactt
    42781 ttgatcctca aactggcgtt caaaagcgta gacttgtttg ctatcttcaa aggctggttt
    42841 gtaacttcct tctgaaatga ttggcatttc cttacgcagt cgaatcaagt cttgatagaa
    42901 ggtaaaaatc ggaccctgga tttcattttc tacattgatg tatttatagg atttaccagc
    42961 tttcaaccaa ggagtgcctg ttgaaaatcc tgcattttcc gaagcatccc actgcatggg
    43021 aatgcgtgaa ttatcacgcg acttagcttg aataatctgg aaggcttctt gctgactctt
    43081 tccttcttct aagagcatct gataggcatt aagcgattcg acatccacat aatcagccat
    43141 agaatcatag tctgggtcaa tcatcccgat ttcctcaccc atgtagatat aaggtgtccc
    43201 acgtgacagg tgaatgctgg ctgctagcat ggtggctcct tccttgcgga agttttgaat
    43261 atcgacaaaa cggttcaagg cacgtggttg atcgtgatta ttccaaaaga gggcactcca
    43321 accgtcttta tcactcattt ccttacccca actatggtaa agactcttca actcttcaaa
    43381 atcaaaggga gccaaggtcc acttttgtcc atccttatag tccaccttga ggtgatgaaa
    43441 attaaaggtc atggataatt cctgacgatc aggcgacgaa tagaggacac agttttccat
    43501 ggtggtagaa gacatttccc caactgtcat aaagctatcg tcggatccaa aagtggcttg
    43561 gttcatcata cgcaaatagt tatgaacgat gggtttgtct gtataagctg gcttcccttc
    43621 attttcagga cagtccactg aaacctcgtc cttaccgatc aaattgatca catcaaatcg
    43681 gaaacctttg acacccttgt cgcgccagaa attaacaacc ttgaaaagct ccttacggac
    43741 attggaattg cgccagttaa ggtcagcctg ggtctcatca aataggtgaa gatagtattt
    43801 cccagtatcc ccgaaaggcg tccatgcaga accaccaaac ttagactgcc aatctgttgg
    43861 ttggtcttgg atgaagaaaa agtcttgata atacttatca ccagctaggg ctttctgaaa
    43921 ccattcatgc tctgtcgaac aatgattaag taccatgtcc agcataaagt caatcttgtg
    43981 ctctttaccg acacacacca ttttctcaaa atcagccata tcaccaaaaa gaggatccac
    44041 tgccatataa tctgaaatat cgtaaccatt atcccgttga gggcttggat agaatggatt
    44101 gagccagacc atatccacac ctagtttggc taaataggga attttttcga taatcccacg
    44161 gaaatcccca ataccgtttt cagtggtgtc tttgtaagat tttggataga tttgatagac
    44221 tacttttcct ttatcaagtg tcatctgttt ctccttttct gataaaaggg aggaagcagt
    44281 cttccgtccc tatttgtgct atttcaatta tactcaatga aaatcaaaga acaaactagg
    44341 aagctagcca caggttgctc aaaacactat tttgaggttg cagatagagc tgacgtggtt
    44401 tgaagagatt ttcgaagagt attagattcg tgtagcgacc atgagagatg ctccagcttg
    44461 gatcgttgtc ggataagttc cgggaatagt cgctgtataa gcatcttggt tggtgatgat
    44521 aacaggagtt tctgtcacca gacctgcagc cttaatgaca tccatatcaa aacgaatcag
    44581 ttgctgacca actgtaacgt gatctccttg gactacaaga ctttcaaaac ctttgccatc
    44641 aagacctact gtatccatac cgatgtggat gagcaattca actccctcgt cagagacaat
    44701 gccgatggca tgcttggtag ggaaaagaac cgtcactgtc ccattaactg gagaggtcaa
    44761 ctcaccttgg cttggttcaa tgactagacc ttgccccatg acacctgatg caaaaatagg
    44821 atccgtcgct tgactcaatt ctttcacttg gccagttagt gggctgataa tttctaccga
    44881 agtaagttct actggttcat ggttcacaaa ttctgcttct tcttgagcaa cgaattctgc
    44941 ctgcaagttc gtatcgccct ctgtttttgt aaagagacca gccttgcgga agaagaaagt
    45001 caagagcatt ggaacaacaa tcgcaactag catagttcct gcaaatggca gcatgtattg
    45061 aggttgaata gagagaatac ctggcaaacc accgatacca atagaagccg cagttacatt
    45121 aaaagtaacg gataacatgc ctgcaagggc tgaaccagtc atcccagcaa caaatggata
    45181 aatatatttt acgttaaccc caaaaagagc tggttctgta acaccgagat aggctgaaat
    45241 ggttgcagga agtgaaacct gagcctcacg ctcatcatgg cgatgcatga aataataggc
    45301 aaacacggct aagccttgag caatattaga aagagcaatc attggccata gggcagtgcc
    45361 accagcatcc gcaatcaatt gtgtatcaat ggcattggtc atatggtgca gacctgtgat
    45421 gacaaatgga gcgtagaggg cgccaaaaat tgcaccgaag agccatttaa ctggaccagt
    45481 taaacctgcc aagacaactg atgaaagtcc ttgtccaatt gtccaaccga ttggtcccaa
    45541 aacagtatga gccaaaatca aggctggaat caatgacaag aaaggtacaa aaatcataga
    45601 aatgacttct gggatatgct tgtgccagaa gatttcaaga taagacagac tcaaacctgc
    45661 aagcaaggct gggataactt gggcttggta accgatacga ttaacagtaa aatagccaaa
    45721 attccaaacc cagtttgccg cgatatcagc tgctggcgtt gaagcaaccg cataggcatt
    45781 gagcaactga ggcgatacca aacagattcc gagaacaatt cccaaaattt ggctggttcc
    45841 catcttacga gaaacagacc aagtaatccc tactggtaag aactggaaga tagcttcacc
    45901 aggcaaccag aggaagtgat tgacacctgc ccaaaactga gaggattctg tgatggtctt
    45961 gccatccaac atcgaccaat ggacaccttc caagacatta cggaaaccga ggatcaatcc
    46021 tccgactatc aaggctggaa taatcggagt aaaaatctcc gccagagtgg tcataacacc
    46081 ttggaccacg ttttgattac tcttagctgc agacttggct gcttctttgg aaacaccctc
    46141 aatacctgaa acggctgtaa aatcattata aaagatgggc acgtcatttc caatgattac
    46201 ctgaaattga cctgcatttg taaaggttcc tttaacagct ggaattgact cgatagcttt
    46261 aacattagcc ttcttatcat ctcctaaaac aaaccgcatc cgtgtcgcac agtgagttac
    46321 ggcagtcaca ttttctttgc ctccgattgc ctgaagcaga tctttggctt cttgttcaaa
    46381 ttttcccatt tgattctcct tatacttgtc aaagcaagtt tgatttgata agtatattgt
    46441 aaacgatacc aaacttgtct gcaacttgtt tggtataaaa agggtgtttt tttgtaaatt
    46501 ttctagcact tgtatggtaa aatagataca ggtgttcatt aaactagact aaaaacctat
    46561 ttaagcaggc aaaatgaaga aataccaaca attatttaag caaatccaag aaaccattca
    46621 aaacgagact tacgctgtcg gagatttcct tcctagcgag cacgacctta tggagcaata
    46681 tcaagtgagt cgtgataccg tccgaaaggc cctgtctctc ctccaagagg aaggattgat
    46741 caaaaagata agagggcaag gttctcaagt cgtcaaagaa gaaaccgtca atttccctgt
    46801 atccaaccta accagctacc aagaactagt taaagaactt ggactgcgct ctaaaaccaa
    46861 cgtggtcagt ctggacaaga ttattattga taaaaaatcc tcactgataa ccggtttccc
    46921 agagtttcgg atggtttgga aggtggtccg ccagcgtgtg gtggatgatc tggtatccgt
    46981 tctggatacg gactatctgg atatggaact catcccaaat ctcactcgcc aaattgctga
    47041 gcagtctatc tattcttata tagaaaatgg cctcaaactc cttattgatt atgctcagaa
    47101 ggaaatcacc attgaccact caagcgaccg agacaagatt ctcatggaca ttggcaaaga
    47161 cccttatgtc gtttcgatta aatcaaaagt ctatctccaa gacggacgcc aatttcagtt
    47221 taccgaaagt cgccataagt tagagaaatt tagatttgta gattttgcaa aacgcaagaa
    47281 ataaaagact gagacaccag atctcagcct ttttcggctc tataatattt gtagtgggta
    47341 acccccctat ggatattatg gagcctattt tgtgtagaaa aaaagtccca tatgacctat
    47401 aatgaaaagc gacaaaacaa ctcattagaa agattcatat ggaacaatta cattttatca
    47461 caaaactgct cgatattaaa gacccaaaca tcaagattct aga
//
LOCUS       DAVKNL010000011        47019 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVKNL010000011 DAVKNL010000000
VERSION     DAVKNL010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 47019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47019
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.011"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            650..2803
                     /locus_tag="VW411_000577"
     CDS             650..2803
                     /locus_tag="VW411_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEU9793363.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPHKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIF
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGSVNLNQIDKKALRQYINYLSQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLIALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2816..4165
                     /locus_tag="VW411_000578"
     CDS             2816..4165
                     /locus_tag="VW411_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEU9793364.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGASLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKVTASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKYVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4335..5042
                     /locus_tag="VW411_000579"
     CDS             4335..5042
                     /locus_tag="VW411_000579"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU9793365.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNVAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQELK"
     gene            5044..5187
                     /locus_tag="VW411_000580"
     CDS             5044..5187
                     /locus_tag="VW411_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU9793366.1"
                     /translation="MFATENLRLSTKRTQAEKVPQTFSLCRELGELTDVYEIVWEKLQ
                     GLK"
     gene            5244..8969
                     /locus_tag="VW411_000581"
     CDS             5244..8969
                     /locus_tag="VW411_000581"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEU9793367.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLAEDLFAPAEKHIFSEQVTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYAAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGATPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDAFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWFKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLA
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEAIQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELETALTAQLGGFVFTSPEEIAGVEKVG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEAANSTSPTLFYNDANQHVAKMVETRIANTNSPWLVGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            9062..10504
                     /locus_tag="VW411_000582"
     CDS             9062..10504
                     /locus_tag="VW411_000582"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU9793368.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAVCSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10723..11745
                     /locus_tag="VW411_000583"
     CDS             10723..11745
                     /locus_tag="VW411_000583"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEU9793369.1"
                     /translation="MANKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKTLEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11742..12287
                     /gene="purN"
                     /locus_tag="VW411_000584"
     CDS             11742..12287
                     /gene="purN"
                     /locus_tag="VW411_000584"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEU9793370.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKADYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            12371..12880
                     /locus_tag="VW411_000585"
     CDS             12371..12880
                     /locus_tag="VW411_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU9793371.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLIFFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLLSV"
     gene            12905..14452
                     /gene="purH"
                     /locus_tag="VW411_000586"
     CDS             12905..14452
                     /gene="purH"
                     /locus_tag="VW411_000586"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEU9793372.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            14574..15836
                     /gene="purD"
                     /locus_tag="VW411_000587"
     CDS             14574..15836
                     /gene="purD"
                     /locus_tag="VW411_000587"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEU9793373.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHYKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVPTATYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGVIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELYQQKIEGLFYRTDIGSKAIK"
     gene            16239..16727
                     /gene="purE"
                     /locus_tag="VW411_000588"
     CDS             16239..16727
                     /gene="purE"
                     /locus_tag="VW411_000588"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEU9793374.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16714..17805
                     /gene="purK"
                     /locus_tag="VW411_000589"
     CDS             16714..17805
                     /gene="purK"
                     /locus_tag="VW411_000589"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEU9793375.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPVSRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLVDKAKAMAVRIAEQLNLSGTLCVEMFATADDIIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEKYVTENPSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVEEFGEGIDF"
     gene            17815..18042
                     /locus_tag="VW411_000590"
     CDS             17815..18042
                     /locus_tag="VW411_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HEU9793376.1"
                     /translation="MIQIIVNTFIEKYKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYNVPLDTDLNTLDHYPSVFIGKEEFE"
     gene            18105..19403
                     /gene="purB"
                     /locus_tag="VW411_000591"
     CDS             18105..19403
                     /gene="purB"
                     /locus_tag="VW411_000591"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU9793377.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(19458..23396)
                     /gene="strH"
                     /locus_tag="VW411_000592"
     CDS             complement(19458..23396)
                     /gene="strH"
                     /locus_tag="VW411_000592"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU9793378.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAVLQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPATEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVTAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLDDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYILGQKPEDGGG
                     FLKKAIENTGKTPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAVTHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQEEEIPFTTVTCENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKVLPQDPAPVVTEKKLPETGTHDSAGLVVAGLMSTLAAYGLTKRKED"
     gene            complement(23724..24440)
                     /locus_tag="VW411_000593"
     CDS             complement(23724..24440)
                     /locus_tag="VW411_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9793379.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
     gene            24789..26576
                     /locus_tag="VW411_000594"
     CDS             24789..26576
                     /locus_tag="VW411_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEU9793380.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPCEGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            26573..27049
                     /locus_tag="VW411_000595"
     CDS             26573..27049
                     /locus_tag="VW411_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU9793381.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILGGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            27077..27982
                     /locus_tag="VW411_000596"
     CDS             27077..27982
                     /locus_tag="VW411_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9793382.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            27969..28784
                     /locus_tag="VW411_000597"
     CDS             27969..28784
                     /locus_tag="VW411_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9793383.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            28791..29195
                     /locus_tag="VW411_000598"
     CDS             28791..29195
                     /locus_tag="VW411_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9793384.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            29494..30660
                     /locus_tag="VW411_000599"
     CDS             29494..30660
                     /locus_tag="VW411_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU9793385.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATVLVSFARSGNSPESLATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTTLLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            30776..31813
                     /locus_tag="VW411_000600"
     CDS             30776..31813
                     /locus_tag="VW411_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEU9793386.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHVYNGTLLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            31930..33143
                     /locus_tag="VW411_000601"
                     /pseudo
     CDS             31930..33143
                     /locus_tag="VW411_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041867.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein C"
     gene            33595..39165
                     /gene="zmpC"
                     /locus_tag="VW411_000602"
     CDS             33595..39165
                     /gene="zmpC"
                     /locus_tag="VW411_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease ZmpC"
                     /protein_id="HEU9793387.1"
                     /translation="MSRKSIGEKRHSFSMRKLSVGLVSVTVSSFFLMSQGIQSVSADN
                     MESPIHYKYMTEGKLTDEEKSLLVEALPQLAEESDDTYYLVYRSQQFLPNTGFNPTVG
                     TFLFTAGLSLLVLLVSKRENGKKRLVHFLLLTSMGVQLLPASAFGLTSQILSAYNSQL
                     SIGVGEHLPEPLKIEGYQYIGYIKTKKQDNTELSRTVDGKYSAQRDSQPNSTKTSDVV
                     HSADLEWNQGQGKVSLQGEASGDDGLSEKSSIAADNLSSNDSFASQVEQNPDHKGESV
                     VRPTVPEQGNPVSATTVQSAEEEVLATTNDRPEYKLPLETKGTQEPGHEGEAAVREDL
                     PVYTKPLETKGTQGPGHEGEAAVREEEPAYTEPLATKGTQEPGHEGKATVREETLEYT
                     EPVATKGTQEPEHEGEAAVEEELPALEVTTRNRTEIQNIPYTTEEIQDPTLLKNRRKI
                     ERQGQAGTRTIQYEDYIVNGNVVETKEVSRTEVAPVNEVVKVGTLVKVKPTVEITNLT
                     KVENKKSITVSYNLIDTTSAYVSAKTQVFHGDKLVKEVDIENPAKEQVISGLDYYTPY
                     TVKTHLTYNLGENNEENTETSTQDFQLEYKKIEIKDIDSVELYGKENDRYRRYLSLSE
                     APTDTAKYFVKVKSDRFKEMYLPVKSITENTDGTYKVTVAVDQLVEEGTDGYKDDYTF
                     TVAKSKAEQPGVYTSFKQLVTAMQSNLSGVYTLASDMTADEVSLGDKQTSYLTGAFTG
                     SLIGSDGTKSYAIYDLKKPLFDTLNGATVRDLDIKTVSADSKENVAALAKAANSANIN
                     NVAVEGKISGAKSVAGLVASATNTVIENSSFTGKLIANHQDSNKNDTGGIVGNITGNS
                     SRVNKVRVDALISTNARNNNQTAGGIVGRLENGALISNSVATGEIRNGQGYSRVGGIV
                     GSTWQNGRVNNVVSNVDVGDGYVITGDQYAAADVKNASTSVDNRKADRFATKLSKDQI
                     DAKVADYGITVTLDDTGQDLKRNLREVDYTRLNKAEAERKVAYSNIEKLMPFYNKDLV
                     VHYGNKVATTDKLYTTELLDVVPMKDDEVVTDINNKKNSINKVMLHFKDNTVEYLDVT
                     FKENFINSQVIEYNVTGKEYIFTPEAFVSDYTAITNNVLSDLQNVTLNSEATKKVLGA
                     ANDAALDNLYLDRQFEEVKANIAEHLRKVLAMDKSINTTGDGVVEYVSEKIKNNKEAF
                     MLGLTYMNRWYDINYGKMNTKDLSTYKFDFNGNNETSTLDTIVALGNSGLDNLRASNT
                     VGLYANKLASVKGEDSVFDFVEAYRKLFLPNKTNNEWFKENTKAYIVEMKSDIAEVRE
                     KQESPTADRKYSLGVYDRISAPSWGHKSMLLPLLTLPEESVYISSNMSTLAFGSYERY
                     RDSVDGVILSGDALRTYVRNRVDIAAKRHRDHYDIWYNLLDSASKEKLFRSVIVYDGF
                     NVKDETGRTYWARLTDKNIGSIKEFFGPVGKWYEYNSSAGAYANGSLTHFVLDRLLDA
                     YGTSVYTHEMVHNSDSAIYFEGNGRREGLGAELYALGLLQSVDSVNSHILALNTLYKA
                     EKDDLNRLHTYNPVERFDSDEALQSYMHGSYDVMYTLDAMEAKAILAQNNDVKKKWFR
                     KIENYYVRDTRHNKDTHAGNKVRPLTDEEVANLTSLNSLIDNDIINRRSYDDNREYKR
                     NGYYTISMFSPVYAALSNSKGAPGDIMFRKIAYELLAEKGYHKGFLPYVSNQYGAEAF
                     ASGSKTFSSWHGRDVALVTDDLVFKKVFNGEYSSWADFKKAMFKQRIDKQDNLKPITI
                     QYELGNPNSTKEVTITTAAQMQQLINEAAAKDITNIDRATSHTPASWVHLLKQKIYNA
                     YLRTTDDFRNSIYK"
     gene            39521..40375
                     /locus_tag="VW411_000603"
     CDS             39521..40375
                     /locus_tag="VW411_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9793388.1"
                     /translation="MTYEYQSHIYLAEAVLNVKDLVSQTVFYQQIIGLEILSQTDTEV
                     VLGLGGKALVHLIQAQEGGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEALYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEVLAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSRQFYQTVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLDPRKQVLPGLAYYVIEVAHKEELLTIAQRAQEVDAPIKWMTSIQLEITDSDGI
                     VTRIRLAR"
     gene            40481..41038
                     /locus_tag="VW411_000604"
     CDS             40481..41038
                     /locus_tag="VW411_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEU9793389.1"
                     /translation="MTSEYQKMIAGEFYRPSDPELRALAQASRQKQAAFNKEENPLKG
                     AEIIKTWFASTGKNLYINTRLMVDYGVNIHLGENFYSNWNLTMLDICPIRIGDNAMIG
                     PNCQFLTPLHPLDPQERNSGIEYGKPITIGDNFWTGGGVIVLPGVTLGNNVVAGAGAV
                     ITKSFGDNVVLAGNPARVIKEIPVK"
     gene            41255..42019
                     /locus_tag="VW411_000605"
     CDS             41255..42019
                     /locus_tag="VW411_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEU9793390.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGANCVGEFVSATKTYPVYVVNYKDEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            42632..44011
                     /locus_tag="VW411_000606"
     CDS             42632..44011
                     /locus_tag="VW411_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU9793391.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLV
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            44025..44690
                     /locus_tag="VW411_000607"
     CDS             44025..44690
                     /locus_tag="VW411_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEU9793392.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            45000..45539
                     /locus_tag="VW411_000608"
     CDS             45000..45539
                     /locus_tag="VW411_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9793393.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKCHGGAVNRGLVEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQELESKGQEVDVFVTNFVYEKEGQSRKKSMSYDSVLPVRQIFGWDQVGNFSKGQ
                     YTMMHSLIYRTDLLRASQF"
     gene            45606..46016
                     /locus_tag="VW411_000609"
     CDS             45606..46016
                     /locus_tag="VW411_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEU9793394.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLIDQLD
                     LSQVSHPKMREYLLNHIELTTVISSTLLNRSGTAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            46132..>47019
                     /gene="pavB"
                     /locus_tag="VW411_000610"
     CDS             46132..>47019
                     /gene="pavB"
                     /locus_tag="VW411_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEU9793395.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADG
                     LNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     SPSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLV"
BASE COUNT        13602 a         8856 c        10846 g        13715 t
ORIGIN      
        1 ttaattagaa aaaaattttt aaagtcttcg gagaagaaaa cagaaaaatc tgttaaaaaa
       61 taatcaaaac tataaatgat gaatctgaat caaaattatt ttgcgcatgt aaagaggagt
      121 cttatagtaa cgagtcaaaa aaggagtaac tatgaatcgt aatttagaac ggtgttatct
      181 attctgacta ggaatagatc ataccagagg tagcttagaa atagcagaga cattagaaat
      241 tgaagtaata aataggatgt cgtaagtgtt actatcaatg atttatttgt ttcaagcttg
      301 cctagggtga cagtaaaaaa tcaatttcct ttcaatagca tatttttagt gggcaggact
      361 cttgttctgc ctattttttt atccaaaaag tgcagttggg agggagatag gctcatttgg
      421 gaaggaagtc cagtttttgt ttagtgattg gggtaagata gttgttatca gatgagttaa
      481 tactcttcga aaatcaaatt caaaccacgt caacgtcgcc ttgccgtata tatgtgactg
      541 acttcgtcag tcctatctac aacctcaaaa cagtgttttg agcagcctac ggctagtttc
      601 ctagtttgct ctttgatttt cattgagtat tagggaaaag gagatgaata tgaaatttgg
      661 gaaacgtcat tatcgtccgc aggtggatca gatggactgc ggtgtagctt cattagccat
      721 ggtttttggc tactatggta gttattattt tttggctcac ttgcgagaat tggctaagac
      781 gaccatggat gggacgacgg ctttgggctt ggtcaaggtg gcagaggaga ttggttttga
      841 gacgcgagcc attaaggcag atatgacgct ttttgacttg ccggatttaa cttttccttt
      901 tgttgcccat gtgcttaagg aagggaaatt gctccactac tatgtggtga ctgggcagga
      961 taaggatagc attcatattg ccgatccaga tcccggggtg aagttgacta aactgccacg
     1021 tgagcgtttt gaggaagaat ggacaggagt gactcttttt atggcaccta gtccagacta
     1081 taagcctcat aaggaacaaa aaaatggtct gctctctttt atccctatat tagtgaagca
     1141 gcgtggcttg attgccaata tcgttttggc aacactcttg gtaaccgtga ttaacattgt
     1201 gggttcttat tatctgcagt ctatcattga tacctatgtg ccagatcaga tgcgttcgac
     1261 actagggatt atttctattg ggctagtcat cgtctacatc ttccagcaaa tcttgtctta
     1321 cgctcaggag tatctcttgc ttgttttggg gcaacgcttg tcgattgacg tgattttgtc
     1381 ctatatcaag catgtttttc acctccctat gtccttcttt gcgacacgca ggacagggga
     1441 gatcgtgtct cgttttacag atgctaacag tatcatcgat gcgctggctt cgaccatcct
     1501 ttcgattttc ctagatgtgt caacggttgt cattatttcc cttgttctat tttcacaaaa
     1561 taccaatctc tttttcatga ctttattggc gcttcctatc tacacagtga ttatctttgc
     1621 ctttatgaag ccgtttgaaa agatgaatcg ggataccatg gaagccaatg cggttctgtc
     1681 ttcttctatc attgaggaca tcaacggtat tgagactatc aagtccttga ccagtgaaag
     1741 tcagcgttac caaaaaattg acaaggaatt tgtggattat ctgaagaaat cctttaccta
     1801 tagtcgagca gagagtcagc aaaaggctct gaaaaaggtt gcccatctct tgcttaatgt
     1861 cggcattctc tggatggggg ctgttctggt catggatggc aagatgagtt tggggcagtt
     1921 gattacctat aataccttgc tggtttactt tactaatcct ttggaaaata tcatcaatct
     1981 gcaaaccaag cttcagacag cgcaggttgc caataaccgt ctaaatgaag tgtatctagt
     2041 agcttctgag tttgaggaga agaaaacagt tgaggatttg agcttgatga agggagatat
     2101 gaccttcaag caggttcatt acaagtatgg ctatggtcga gatgtcttat cggatatcaa
     2161 tttaaccgtt ccccaagggt ctaaggtggc ttttgtgggg atttcagggt caggtaagac
     2221 gactttggcc aagatgatgg ttaattttta cgacccaagt caaggggaga ttagtctggg
     2281 tagtgtcaat ctcaatcaga ttgataaaaa agccctgcgc cagtacatca actatctgtc
     2341 tcaacagccc tatgtcttta acggaacgat tttggagaat cttcttttgg gagccaagga
     2401 ggggacgaca caggaagata tcttacgggc ggtcgaattg gcagagattc gagaggatat
     2461 cgagcgcatg ccactgaatt accagacaga attgacttcg gatggggcag ggatttcagg
     2521 tggtcaacgt cagagaatcg ctttggcgcg tgctctcttg acagatgcgc cggtcttgat
     2581 tttggatgag gcgactagca gtttggatat tttgacagag aagcggattg tcgataatct
     2641 cattgctttg gacaagacct tgattttcat tgctcaccgc ttgactattg ctgagcggac
     2701 agagaaggta gttgtcttgg atcagggcaa gattgtcgaa gaaggaaagc atgctgattt
     2761 gcttgcacag ggtggctttt acgcccattt ggtcaatagc tagaaagagg agaggatgaa
     2821 accagaattt ttagaaagtg cggagtttta taatcgtcgt taccataatt tttccagtag
     2881 tgtgattgta cccatggccc ttctgcttgt gtttttactt ggctttgcaa ctgttgcaga
     2941 gaaggagatg agtttgtcca ctagagctac tgtcgaacct agtcgtatcc ttgcaaatat
     3001 ccagtcaact agcaacaatc gtattcttgt caatcatttg gaagaaaata agctggttaa
     3061 gaagggggat cttttggttc aataccaaga aggggcagag ggtgtccaag cggagtccta
     3121 tgccagtcag ttggacatgc taaaggatca aaaaaagcaa ttggagtatc tgcaaaagag
     3181 cctgcaagaa ggggagaacc actttccaga ggaggataag tttggctacc aagccacctt
     3241 tcgcgactac atcagtcaag caggcagtct tagggctagt acatcgcaac aaaatgagac
     3301 catcgcgtcc cagaatgcag cagctagcca aacccaagcc gaaatcggca acctcatcag
     3361 tcaaacagag gctaaaattc gcgattacca gacagctaag tcagctattg aaacaggtgc
     3421 ttccttggcc ggtcagaatc tagcctactc tctttaccag tcctacaagt ctcagggcga
     3481 ggaaaatccc caaactaagg ttcaggcagt tgcacaggtt gaagcacaga tttctcagtt
     3541 agaatctagt cttgctactt accgtgtcca gtatgcaggt tcaggtaccc agcaagccta
     3601 tgcgtcaggg ttaagcagtc aattggaatc ccttaaatcc caacacttgg caaaggttgg
     3661 tcaggaattg acccttctag cccagaaaat tttggaggca gagtcaggta agaaggtaca
     3721 gggaaatctt ttagacaagg ggaaagttac ggcgagtgag gatggggtgc ttcatcttaa
     3781 tcctgagacc agtgattcta gcatggttgc agaaggtgcc ctactagccc aactttatcc
     3841 atctttggaa agagaaggga aagccaaact cacagcttat ctaagttcaa aatatgtagc
     3901 aagaatcaag gtcggtgatt ctgttcgcta tactacgact catgatgccg ggaatcaact
     3961 tttcctagat tctactatta caagtattga tgcgacagct actaagactg agaaagggaa
     4021 tttctttaaa atcgaggcgg agactaatct aacttcggag caggctgaaa aacttaggta
     4081 cggggtggaa ggccgcttgc agatgattac gggcaagaaa agttacctac gttattattt
     4141 ggatcaattt ttgaacaaag agtaatgttc gtgtttttag agttaaataa tttttaaact
     4201 gtgagaaaga ttcttcttgc agttttttct ttacaatttt tgaaaaacat ctactattta
     4261 ttcggttaaa ttcttgtgtt ttttggtttt ttgtggtaaa atgtgctcaa gtaatacgaa
     4321 aggcgaactt taaaatgtca aaacaattga tctattcggg aaaagctaaa gatatctata
     4381 caactgagga tgaaaatctt attatttcaa cttacaagga ccaggcgact gctttcaacg
     4441 gtgtcaagaa ggagcagatt gcaggtaagg gagtcttgaa taatcagatc tcatctttta
     4501 tttttgagaa attaaatgtg gctggtgtgg cgactcactt tgtggagaaa ctttcagaca
     4561 cggaacaact caataaaaag gttaagatta ttcctttgga agtcgtgctc cgcaactata
     4621 ctgctggttc cttttcaaaa cgttttggtg tggatgaggg aatcgccttg gagactccga
     4681 ttgtcgaatt ttactacaaa aatgatgatt tggatgatcc atttatcaat gatgagcatg
     4741 tgaaattcct acagattgcg ggtgaccagc agattgccta cttgaaggaa gaaacgcgtc
     4801 gtatcaatga actattgaaa gtctggtttg ctgagattgg gcttaaattg attgacttta
     4861 agctagagtt cggttttgac aaggatggca agattatctt ggcagacgaa ttttcaccag
     4921 ataactgccg cttgtgggac gctgatggca accacatgga taaggatgtt ttccgtagag
     4981 gattgggaga actaaccgac gtttatgaga ttgtttggga aaagttgcag gaattgaaat
     5041 aatctgtttg caacggaaaa ccttcgtctc tcaactaaaa ggactcaggc tgaaaaggtc
     5101 ccccagacct tttcactctg tagagaacta ggtgaactaa cagatgttta cgaaattgtc
     5161 tgggaaaagt tgcagggttt aaaataacaa cctcaaggct gtttgggaat attgcaagag
     5221 ctgaaataaa ggaataagaa ttgatggata aacgtatttt tgttgaaaaa aaggctgatt
     5281 ttcaggtcaa gtcagagagt ttggttagag agctccagca caacttggga ctgtcaagct
     5341 tgaaaagtat tcgtattgtg caagtatatg atgtatttga cttggctgag gacttgtttg
     5401 cacctgcaga gaagcacatt ttctctgagc aggtaaccga ccatgtttta gatgaagtat
     5461 ctgtgcaggc ggatcttgct aactatgctt tctttgccat tgaaagtctg ccagggcagt
     5521 ttgaccagcg tgcagcttcg tcacaggaag ccttgctttt gttgggaagt tcgagtgacg
     5581 tgacagtcaa cacagcccaa ctttacttgg tgaataaaga tattgatgcg actgagttgg
     5641 aagctgtcaa aaactacctg ctcaatccag ttgattctcg tttcaaggat atcacgacag
     5701 ggattgccaa gcaggagttt tcagagtcag acaagaccat tcccaaattg actttctttg
     5761 aaagctatgc agcagaagac tttgctcgct acaaggccga acaagggatg gccatggaag
     5821 tggatgattt gctctttatc caagactact ttaagtcaat cgggcgcgtg ccaactgaga
     5881 ctgaactcaa ggttttggac acttactggt ctgaccactg ccgtcatacg acttttgaga
     5941 cagagttgaa acacatcgac ttttcagctt ctaaatttca aaagcaattg cagtcaacct
     6001 atgacaagta tattgccatg cgcgaggaat taggtcggtc tgaaaaacca caaaccttga
     6061 tggatatggc gactattttc ggtcgttatg agcgtgctaa tggacgattg gatgatatgg
     6121 aagtctctga cgaaatcaat gcctgctcag ttgaaattga agtggacgtt gatggtgtca
     6181 aggaaccttg gctcctcatg tttaaaaacg aaacccacaa ccatccaaca gaaattgagc
     6241 catttggtgg agcggctacc tgtattggtg gagctattcg tgatccgttg tcaggccgtt
     6301 cctatgttta ccaagccatg cgtatttcag gtgctggtga tattacagca ccgatttcgg
     6361 aaactcgcgc tgggaaattg ccacaacaag tcatttctaa aacagcagct catggttatt
     6421 cttcatatgg taaccagatt gggcttgcaa caacctacgt tcgtgaatac ttccacccag
     6481 gctttgtagc taaacgtatg gaacttggtg ccgttgttgg tgcgactccc aagggcaatg
     6541 ttgtccgtga aaaacctgaa gcaggtgatg tgatcatcct tctcggaggc aaaacaggtc
     6601 gtgatggtgt cggtggtgcg acgggctctt ctaaggttca aacagttgag tctgtagaga
     6661 ctgctggtgc tgaggttcaa aaaggaaatg ccatcgaaga acgcaagatt cagcgcctct
     6721 tccgtaatgg caatgtcact cgtctgatca agaagtccaa tgactttggg gcaggcggtg
     6781 tctgtgtggc tatcggtgaa ttggcagacg gtcttgaaat cgacctcaac aaggtgcctc
     6841 ttaaatacca gggcttgaat ggtacagaaa ttgccatctc tgaatcacaa gaacggatgg
     6901 cggtcgtggt tcgtcctgaa gatgtggatg ccttcgttgc cgaatgtaac aaagaaaata
     6961 ttgatgctgt tgtggtggcg acagtaactg aaaaaccaaa tcttgtcatg cactggaatg
     7021 gtgagacaat cgttgacttg gagcgtcgtt tccttgacac caatggtgtg cgcgtggttg
     7081 tcgatgccaa agttgtggac aaggatgtca aactcccaga agagcgtcaa acatctgctg
     7141 aaacactgga atcagatacc cttacggttc tatctgacct caaccatgca agtcaaaaag
     7201 gattacagac tatctttgac tgctctgttg gacgctcaac ggttaatcac ccacttggtg
     7261 gtcgttacca actcacacca actgaggcat ctgtgcagaa attgccagtt caacacggtg
     7321 tgactcatac tgcgtcggtc attgctcaag gtttcaaccc atatgtagct gaatggtctc
     7381 cataccacgg tgctgcttat gcggttatcg aagcaactgc tcgtttggtg gctgctggtg
     7441 ccaactggtt caaggctcgt ttctcttacc aagagtattt cgagcgtatg gataaacaag
     7501 cagagcgttt cggtcagcca gtagctgctc ttctaggttc tattgaagca caaattcagc
     7561 ttggcttgcc atctatcggt ggtaaggact ccatgtctgg tacctttgaa gaattgaccg
     7621 ttccgccaac cttggctgcc tttggggtga cgacggcaga tagccgtaag gtgctctctc
     7681 cagaatttaa agctgttggg gaaaatatct actacatccc aggtcaagcc ctctctgcag
     7741 agattgattt tgacttgatt aagaaaaatt ttgctcagtt tgaagccatc caagctgacc
     7801 ataaagtgac atctgcatca gctgtcaaat acggtggtgt agttgaaagt ttggctcttg
     7861 ctacctttgg aaactatatt ggtgcagagg tgaccttgcc tgaacttgaa acagctttga
     7921 cagctcaatt aggcggcttt gtcttcacat ctcctgaaga aattgctgga gtagagaagg
     7981 ttggacaaac gaaagcagac tttacactga ctgtcaacgg tgtgaagcta gatggacaca
     8041 agcttgacag tgcatttcaa gggacattgg aagaagttta cccaacagaa tttacccaag
     8101 cgaaagaact agaagaagta ccagctgtgg catcagatgt tgtgattaaa gccaaagaaa
     8161 aggttgaaaa acctgtggtt tacatcccag tctttccagg aaccaactca gaatatgatt
     8221 cagctaaggc cttcgaaaaa gaaggtgcag aggtcaattt ggtgccattc gtgaccttga
     8281 atgaagaagc tattgtcaag tcagttgaaa ctatggttga caatatcgac aagactaata
     8341 ttctcttctt tgctggtgga ttctcggctg cggatgaacc agatggttca gctaagttta
     8401 tcgtcaatat cctgcttaat gaaaaagtgc gtgtggctat tgatagcttt atcgcccgtg
     8461 gtggtttgat tatcggtatt tgtaatggat tccaagcctt agtcaaatcg ggtctcctac
     8521 cctacggaaa ctttgaagct gctaacagta ctagcccaac cctcttctac aatgatgcca
     8581 accaacacgt ggccaagatg gtggaaactc gcattgccaa taccaactca ccatggttgg
     8641 ttggtgtgca agtgggcgat atccacgcta ttcctgtttc gcacggtgaa gggaagtttg
     8701 tcgtgacggc tgaggaattt gcagagctcc gtgacaatgg acaaattttc agccaatacg
     8761 ttgactttaa cggtaaacca agtatggatt ctaagtacaa tccgaatggt tctgtccatg
     8821 ccatcgaagg aattaccagc aagaatggtc aaatcatcgg taagatgggc cactcagaac
     8881 gttatgagga tggtcttttc caaaatatcc caggcaataa agaccaacac ctgttcgcat
     8941 cagcggttaa acatttcact ggaaaataag acttacagat tttctaatag atagtatcag
     9001 taatgtaaaa gtcatgtaaa tctagctctt gatgattaca aatgaaaatt aggtataaaa
     9061 aatgacatac gaagtaaaat ctcttaatga agaatgtggt gttttcggta tttggggaca
     9121 tccagatgct gctaagttga cctattttgg actccacagt cttcaacacc gtggtcagga
     9181 gggggcagga atcctctcca atgatcaagg acaactgaag cgccatcgtg acatggggct
     9241 tttatcagaa gttttcagaa atccagctaa tttggataaa ttgacaggag ctggtgcgat
     9301 tgggcatgtg cgttatgcga ctgctggcga agcttctgta gataacatcc agcccttcct
     9361 cttccgtttt cacgatatgc agtttggttt ggctcataat ggaaatctga ccaatgcagc
     9421 ctctctcaag aaagaactgg aacaaagagg agcaattttc agcgcgactt cggactctga
     9481 aatcttggct cacctcattc gtcgcagtca taatcctagc ctgatgggca aaatcaagga
     9541 agcgctcagc cttgtcaaag gtggttttgc ctatatcttg ctgtttgagg acaagttgat
     9601 tgcggctctt gacccaaatg gattccgacc gctttcgatt ggtaaaatgg ctaatggagc
     9661 agttgttgta tcttctgaaa cctgtgcttt tgaggtcatt ggtgccgagt ggattcgtga
     9721 tttgaagcca ggtgagattg tgatcattga tgacgagggc attcagtatg acagctatac
     9781 agatgatacc cagttggcgg tttgttctat ggagtatatc tactttgctc gccctgattc
     9841 taatatccac ggtgtcaatg tccatacggc acgtaagaga atgggagcgc aattggcgcg
     9901 agaatttaag catgaggcag atattgtagt tggtgtgccc aattcttccc taagcgcggc
     9961 tatgggattt gcggaagaat caggcttacc aaatgaaatg ggtctgatca aaaaccaata
    10021 cacccagcga acttttatcc aaccgactca agaattgcgg gagcaaggag tgcggatgaa
    10081 actgtctgct gtttcgggtg ttgtcaaagg caaacgtgtg gtcatggtgg atgattccat
    10141 tgtacgtgga acaacctctc gtcgtatcgt tcagctcttg aaagaagcgg gtgcgactga
    10201 ggttcacgtt gccattggaa gtcctgcact agcgtatcca tgtttctacg ggattgatat
    10261 ccagacccgt caggagctga ttgcagccaa tcatacggtc gaagaaactc gccaaatcat
    10321 tggtgcggac agtctgactt atctttcaat tgatggcttg attgagtcga ttggtatcga
    10381 aacagatgcg ccgaacggtg gtctctgtgt cgcttacttt gacggtgact acccaacgcc
    10441 tctttatgac tacgaagaag actatcgtag aagtttggaa gaaaagacca gtttttacaa
    10501 gtaggcgaca gattctccat taaagaaaag gaaaaaataa atgacaaata aaaatgcata
    10561 tgcctcacgt ctcactactg actaaaggct taagcattta gtcagtagac gctttgtcct
    10621 ataggatcaa agctagagcc ctgactagta tttttagata aaaagatggt ttatctaaaa
    10681 atacgtcgca gtctttctca aaaaaagaaa aggaaaaata aaatggcaaa taaaaatgcg
    10741 tacgctcaat ctggtgtgga tgttgaagcg ggttatgaag ttgttgaacg gattaaaaag
    10801 cacgtggccc gtacggagcg tgcaggtgtc atgggagctc ttggtggctt tggtggtatg
    10861 tttgaccttt ccaagactgg ggttaaagaa cccgtcttga tttcagggac tgacggtgtc
    10921 ggaaccaagc tcatgttggc tatcaagtac gacaagcacg ataccatcgg gcaggactgt
    10981 gtggccatgt gtgtcaacga catcattgct gcaggtgcgg aacccctcta ttttctcgac
    11041 tacgtagcga cagggaagaa tgaaccagct aagctagaac aagtggttgc tggtgtggca
    11101 gaaggttgtg tgcaggctgg tgctgccctc atcggtgggg aaacggctga aatgccgggc
    11161 atgtacggcg aagacgacta tgacttggct ggttttgcgg tcggtgtggc tgaaaaatct
    11221 caaatcattg acggttcaaa ggtggtagag ggagatgttc ttctcggact tgcttcaagt
    11281 gggattcact caaatggtta ctctttggtt cgtcgtgtct ttgcggatta cacaggtgag
    11341 gaagtcctac cagaattgga aggcaagaaa cttaaggaag ttctacttga gccgactcgt
    11401 atctatgtca aggctgtctt gccgctcatc aaagaagagt tggtcaacgg cattgcccac
    11461 atcacaggtg gtggctttat cgaaaatgtc cctcgtatgt ttgcagatga cctagctgct
    11521 gaaattgatg aaagtaaagt tccagtgctt ccaattttca aaacccttga aaaatacggt
    11581 cagattaaac acgaagaaat gtttgaaatc ttcaatatgg gtgtgggact tatgttggcg
    11641 gtcagccctg aaaatgtaga gcgtgtaaaa gaattgttgg atgaagcagt ctatgaaatt
    11701 ggtcgcatcg tcaagaaaga aaacgaaagt gtcattatca aatgaaaaaa atagcggttt
    11761 ttgcctctgg taatggctca aattttcagg tgattgccga agaatttcca gtggagtttg
    11821 tcttttcaga ccatcgtgat gcctatgtgc ttgagcgtgc aaagcagctc ggcgttctgt
    11881 cctatgcttt tgaactcaag gagtttgaga gcaaggcaga ctacgaagca gcccttgtcg
    11941 aactcttgga agaacaccag attgacttgg tttgcctagc aggctacatg aaaatcgttg
    12001 gaccaacctt attgtcggct tatgaaggtc ggattgtcaa cattcatcca gcctacttgc
    12061 cagaatttcc aggagctcat gggattgagg atgcttggaa tgctggcgtg ggtcagtctg
    12121 gtgtgaccat tcactgggtg gattcgggtg tggatacagg ccaggtcatc aaacaggttc
    12181 gtgtgccacg actagctgat gataccattg acagatttga agctcgcatc catgaagcag
    12241 agtacaggct gtatccggaa gtagtgaagg ctctatttac agattgactt tttgatgatt
    12301 catatgatat ctttgatttt aaattggagt cagtgtttgt tgaagacggc ttcaaacgga
    12361 ggtatttgta atgttagaat ctaaaaaaac aactcgatat gtattttatg tctatctgat
    12421 gttattaact tggggaatct tatttaagtt tgaaacaaat cctgaattta tagcattttt
    12481 cttagctcca aggtatatca attggattcc attttcagaa ccactaatag tcgatggaaa
    12541 aattgttttt gctgaaatgt tatttaatct gattttcttt attccattag gtgtttgttt
    12601 ccctttgata aaaactaatt tatctagttt aagaatagtc gggacaggtt tcttgattag
    12661 tttattgttt gagtgcttac agtatatttt agcaataggt ataacagata taacggattt
    12721 gactttaaat acgctaggtg tctgtgtagg cttactgatt tatcaaattt ttataagagt
    12781 gttcaaatca cagactagaa aatggatcaa tatcttaggt atgcttagcc ttggttttgc
    12841 ttatcttgtt ttactgttac tgcatttact tagtgtttaa ctaatgatta aaaaggagaa
    12901 tataatgact aaacgcgtct taatcagcgt ctcagacaaa gcgggcattg ttgaatttgc
    12961 ccaagaactc aaaaaacttg gttgggagat tatctcaaca ggtggaacta aggttgccct
    13021 tgataatgct ggggtggata ccattgctat cgatgatgtg actggtttcc cagaaatgat
    13081 ggacggtcgt gtgaagaccc tccacccaaa tatccacgga gggcttctcg ctcgtcgtga
    13141 cttggatagc cacttggaag cggctaagga caacaagatt gagctcattg accttgtggt
    13201 ggtcaacctt tacccattta aggaaactat ccttaaacca gatgtgactt atgctgatgc
    13261 agttgaaaat atcgatattg gtgggccatc tatgcttcgt tcagcagcga aaaatcatgc
    13321 cagtgttaca gttgtggtag atcctgctga ctacgctgtg gttttggatg aattggcagc
    13381 aaacggcgaa acctcttatg aaactcgcca acgtttagca gccaaagtat ttcgtcacac
    13441 agcggcttat gacgccttga ttgcagaata cttcacagct caagtgggtg aaagcaagcc
    13501 tgaaaaactc actttgactt atgacctcaa gcaaccaatg cgttacggtg agaatcctca
    13561 acaagacgcg gacttttacc agaaagcttt gcctacagac tactccattg cttcagccaa
    13621 acagctcaac gggaaagaat tgtcatttaa taatatccgt gatgcagatg ctgctatccg
    13681 tatcatccgt gacttcaaag atagtccaac cgttgtggct ctcaaacaca tgaatccatg
    13741 tggaattggt caagctgatg acatcgagac tgcttgggac tacgcttatg agtctgaccc
    13801 agtgtctatc tttggtggga ttgtcgtcct caaccgtgag gtggatgctg cgacagctga
    13861 gaagatgcac ggcgttttcc tcgaaatcat cattgcacca agctatacgg atgaagcgct
    13921 agccattttg atcaataaaa agaaaaactt gcgtatcctt gccttgccat ttaatgctca
    13981 agaggctagc gaagtggaag cagaatacac aggtgtagtc ggtggacttc tcgtgcaaaa
    14041 tcaagacgtg gtcaaggaaa gcccagctga ctggcaagtg gtgactaaac gtcagccaac
    14101 tgagacagaa gcgactgctc ttgagttcgc ttggaaggct atcaagtacg tcaaatcaaa
    14161 tggtattatc gtgaccaacg accacatgac acttggtgtt ggtccaggtc aaaccaaccg
    14221 tgtggcttct gttcgccttg ccattgacca agccaaagat cgtctggacg gggcggtcct
    14281 tgcttcagat gccttcttcc catttgcgga taacgtggaa gaaatcgcca aagcaggaat
    14341 taaggccatc atccagcccg gtggctctgt ccgtgaccaa gaatccatcg aagcagcgga
    14401 taaatacggc ttgactatgg tctttacagg tgtgagacat tttagacatt aagaagataa
    14461 aagggaagaa aacagtttct ttcctttttt ggcttaaaat actaactgaa acaagattaa
    14521 aacgaacttt tttgatataa tgttggtaaa taattcgcaa aagaggttga ggaatgaaac
    14581 tgcttgttgt cggttctggt ggtcgtgagc atgcgattgc taaaaagtta cttgaatcaa
    14641 aagacgtgga aaaagtcttt gtagctcctg ggaatgatgg gatgactctg gatggtttgg
    14701 aattggtaaa tatctctatt tccgaacatt ataaattgat tgacttcgca aagaccaatg
    14761 atgttgcttg gacctttatc ggtccagatg atgcccttgc tgctggtatc gtggatgatt
    14821 ttaaccaagc tggacttaag gcctttggtc cgactagggc tgcagcggag ctggagtggt
    14881 ccaaggattt cgccaaggaa atcatggtca aatacggcgt tccgacagca acatatggca
    14941 cattttcaga tttcgaggaa gccaaagcct atatcgaaaa gcatggtgcg cctatcgtag
    15001 tcaaggcgga tggcttggca cttgggaagg gtgtcgtcgt tgctgagacg gttgagcaag
    15061 cggtcgaagc cgctcatgag atgcttttgg acaataaatt tggtgactca ggtgcgcgcg
    15121 tggttattga ggaattcctt gaaggagagg aattttcact ctttgccttt gtcaatggtg
    15181 ataagttcta catcatgcca acggctcagg accacaaacg tgcctatgat ggcgacaaag
    15241 ggcctaacac gggtggtatg ggtgcctatg cgccagtccc acacttacca cagagtgtag
    15301 ttgatacagc ggttgacacc attgtcaagc cagttctaga aggggtgatt aaagaaggtc
    15361 gcccttatct gggagttctt tacgcagggc ttatcctgac agctgatgga ccgaaagtca
    15421 ttgagttcaa cgctcggttc ggagatccag aaactcagat tatcttgcct cgcttgacct
    15481 ctgactttgc tcaaaatatc acagatatcc tggatagcaa ggagccaaat atcatgtgga
    15541 cggacaaggg tgtgactctg ggtgtggttg tcgcatccaa gggctacccg ctagactatg
    15601 aaaggggcgt tgagttgcca gccaagacag aaggcgatgt catcacctac tatgcagggg
    15661 ctaagtttgc ggaaaatagc agagcactgc tctcaaacgg cggacgagtt tatatgctcg
    15721 ttaccacagc agataccgtc aaagaagccc aagccagcat ataccaagaa ctataccaac
    15781 aaaaaataga aggactcttc taccgaacag atatcggaag caaggcaatt aagtaaagat
    15841 ataagaataa cgcgccgtag tcgccaaaca cgataatggt cgtcgtggtg aaaagaccag
    15901 aacagtgaat gttctggtca gggggaaact tggagacctt aggctcaaag tttaggaatg
    15961 aaaccgaagg tttgcttccg cctccatcac ctaagaccat tatcaaaaag aaaaataaaa
    16021 attcacaaaa tacgttaatg atcgtatggt ttgcgagcgt tagcgagcta atatagaaca
    16081 atcaccgccg ttgtgaaaga acgattggat gataatccaa tcgttcaggg aaattggaag
    16141 accttgggtt tccaatttag gcatgagaca cctttggtgg ctgctgccgt ccctcacaag
    16201 ctaaggtgat tgttgaaaaa gaggaaaaag gagaagaaat gaaaccagta atttccatca
    16261 tcatgggctc aaaatccgac tgggcaacca tgcaaaaaac agcagaagtc ctagaccgct
    16321 tcggtgtagc ctacgaaaag aaagttgttt ccgcacaccg tacaccagac ctcatgttca
    16381 aacatgcaga agaagcccgt agtcgtggca tcaagatcat catcgcaggt gctggtggcg
    16441 cagcgcattt gccaggcatg gtagctgcca aaacaaccct tccagtcatt ggtgtgccag
    16501 tcaagtctcg tgctcttagt ggagtggatt cactctattc tatcgttcag atgccgggtg
    16561 gggtgcctgt tgcgaccatg gctatcggtg aagctggagc gactaacgca gctctctttg
    16621 ccctccgtct cctctctgta gaagataagt ccattgcgga tgcacttgcc aactttgctg
    16681 aagaacaagg aaaaatcgca gaggagtcgt caaatgagct catctaaaac aatcggaatt
    16741 atcggtggcg gtcaactggg tcagatgatg gccatttctg ctatctacat gggccacaag
    16801 gttatcgcgc tggatcctgc ggcggattgc ccggtctctc gtgtggcgga aatcattgtg
    16861 gcaccttata acgatgtaga cgccctccgt cagttggcag accgttgcga tgtcctcact
    16921 tatgagtttg aaaatgtcga cgctgacggt ttggatgccg ttatcaagga tggacaactc
    16981 cctcaaggaa cagatctgct ccgcatttcg caaaatcgta tttttgaaaa ggactttttg
    17041 tcaaacaagg ctcaagtcac tgtggcaccc tacaaggtcg tgacttctag cctagacttg
    17101 gcagatatcg acttgtcgaa aaactatgtc ctcaagactg cgactggtgg ctacgatggt
    17161 catggacaaa aggttattcg ttcagaagca gacttggaag cagcctatgc gctagcagac
    17221 tcagcagact gcgtcttgga agaatttgtc aactttgacc ttgagatttc tgtcatcgtg
    17281 tcaggaaatg gcaaggaggt gacgtttttc ccagttcagg aaaatatcca ccgcaacaat
    17341 atcctgtcta agaccatcgt accagcccgc atttctgaaa gtctagtaga caaggctaaa
    17401 gctatggcag tgcgaatcgc agaacaactc aacttgtctg gaactctctg tgtggaaatg
    17461 tttgcgacag ctgatgacat cattgtcaat gaaatcgccc cacgaccaca taactctggg
    17521 cactattcta ttgaagcctg tgatttctct cagtttgaca cccatattct gggtgttctc
    17581 ggagcaccat taccagtcat caaactccat gcgccagccg ttatgcttaa tgtcctcggt
    17641 cagcatgtcg aggctgctga aaaatatgtc acagaaaatc caagcgccca cctccacatg
    17701 tatggtaaaa tagaagcaaa gcataatcgt aagatgggac atgtgacttt gtttagtgat
    17761 gtgccggata gtgtggaaga gtttggggaa gggattgatt tttaggacaa gtctatgata
    17821 caaattatcg ttaatacatt tattgaaaag tataagactg gagcagttgt tgaagtgttg
    17881 tatgccagtg ctgaccaaga taaggtacaa gctaaatatg aagaactagc tgcacaatac
    17941 cccgaaaatt atttagctat ctataatgta ccgctggata cggatttgaa tacactagat
    18001 cattacccgt ctgtgtttat tggaaaagag gagtttgagt agaaatcttg gtttacctag
    18061 atagcttatt cccaacagct taagaagaaa ggaaaaatta acacatgatc aaccgttact
    18121 ctcgccctga gatggcgaat atttggagtg aagaaaataa ataccgtgct tggcttgagg
    18181 tggaaatcct ctctgacgag gcatgggctg agttggggga aatccctaag gaagatgtgg
    18241 ctttgattcg caagaaggcg gactttgaca tcgaccgtat tttggaaatt gagcaggaga
    18301 cgcgccacga tgtggtggct ttcacgcgtg cggtttctga gactcttggt gaagagcgca
    18361 agtgggttca ctatgggtta acttctactg acgtggtgga tactgcttat ggttacctct
    18421 acaagcaggc caacgacatc atccgtcgtg accttgaaaa cttcactaat atcatcgctg
    18481 acaaggccaa ggagcacaag ttcaccatca tgatggggcg tactcatggt gtgcacgctg
    18541 agccgacaac ctttggtctt aaattagcaa cttggtacag cgaaatgaaa cgcaatatcg
    18601 agcgcttcga gcatgcggct gctggtgtag aagctggtaa gatttctggt gcggttggga
    18661 actttgccaa tatcccacca tttgtagagg agtatgtctg cgataaactt ggcatccgtg
    18721 cccaagaaat ctctacacaa gtccttcctc gtgaccttca cgctgagtac tttgcggttc
    18781 ttgccagcat tgcgacttca atcgaacgta tggcgactga gattcgtggt ctacaaaaat
    18841 ctgagcaacg cgaagtagaa gagttctttg ctaaagggca aaaagggtct tcagcaatgc
    18901 ctcacaaacg caacccaatc ggttctgaaa atatgactgg tctggcgcgt gtcattcgtg
    18961 gtcacatgat tacggcttat gaaaacgtcg ctctctggca tgaacgcgat atttctcact
    19021 catcagctga gcgtatcatc acaccagata cgaccatttt gattgactac atgctcaacc
    19081 gttttggaaa tatcgtcaag aacttgacag tcttcccaga aaatatgatc cgaaacatga
    19141 actcgacttt tggtcttatc tttagccaac gggctatgtt gacattgatt gaaaaaggca
    19201 tgacccgtga gcaagcctat gacttggtgc aaccaaaaac agcctactct tgggacaacc
    19261 aagtagactt taaaccactt cttgaggcag attcagaagt aacatcacgt ctcacacaag
    19321 aagaaatcga tgaaatcttc aacccagttt attacaccaa acgagtggat gatatctttg
    19381 aacgtcttgg actaggtgat taattaaaaa ataaacagcg agcttcaatc tcgctgttta
    19441 ttttttatcg aaaagactta gtcttctttt cttttagtga gtccataggc tgctagtgtg
    19501 gacatgagtc ctgcgactac tagtcctgca gaatcgtgag ttcctgtttc aggaagtttt
    19561 ttctctgtta ccacaggagc tggatcttga ggaagaactt tgctttcctc agcaggagca
    19621 gttgatggag ctggttggct tgggatttct agttttggtt tttcttcagc aatagcggct
    19681 tgtccgtttt catcgcctac atgtgttacc atagttccga cttcgactat ttgagtaacg
    19741 gcttcctgtg ctacgacact atttacaagt gttttcactt ccttaccatc ggcagaagtg
    19801 ctcacagagt agaagttgct acgatgtcca ttgacgccct tagtaatgac ttgtgttttt
    19861 cctttgagta agagtggatt ttcacaagtc actgtggtaa atggaatttc ttcttcttgg
    19921 atatccagtc taggttttac ctcagtagtt ggtgcaagac cactttcatc acccttgtga
    19981 gttacaggag cgccaacttc aaccacttgg tttataactt ctttggttac ctggctatca
    20041 aggactgttt ctgttgtttt tccattttca gtgagtacag agatgtaatg agttcgttca
    20101 cctttgactc ctgctgtgat aatattttcc tgaccggctg ggaggttagg attttctttc
    20161 ttgataactt caaatggaat ttcttcagtt cttgtgatga gttctggtct ggtttcaaca
    20221 ttggcagcca cttcattttc atctaggctt cctgaatgag ttacagctgg tttgaggcct
    20281 tgaagagcgg cttttaggtt ggctacaagc gtgtcaagct cagcttgttt attacggttg
    20341 aggttgtaat ttagagctgt tttagctgcg tcaagggcct caagactttc tttactatat
    20401 ccttctaagt ttgtaggaat tttagctaat tcttcgcgga gagcattata attagcacga
    20461 aagtagtctt tgttgtggtc tgcaaaggca gtcatgagtt caaagatttc ctcttccttg
    20521 tattcagcgc ttggtctatc tgcccagatt gaaagcatac ttccgactgt tggaagatct
    20581 acttcaggat atttggtaga agctagttga ttgaatggtg tttttccagt attctcaata
    20641 gctttcttga ggaaaccacc accatcttct ggtttttgac caagaatgta gtaccagtca
    20701 ccgttggtat tcaagaattt atagcctttg cttgctaggt attgaggtga tgcgaggtta
    20761 tatccccacc agcctttaga ccagtaagaa atcaagacat ctttgtcaaa ctgaacatcg
    20821 tccttgtctt catagtagaa gccatcgttg aaggccattg gttgaagccc tctttctttg
    20881 gccatagctg cgagggtgtt ggcatattcg gcaaatttgc catagagttg ataccacttg
    20941 aggtagtacc agccttgggc actagtcgca tcgttggcgt attcgtcagt accaaagttg
    21001 aaaatctttg ttttacctgc aaagaagtcc atgtatttac cgatgagggc ttttacaaag
    21061 ttcatcgctt cttcgttttt caagtccata gttgtttttg aaactttatc aaagtgggct
    21121 tgaggatttt taatacctaa tttttccatg gcaaccagca tagcatccat gtgacctgga
    21181 ctgttaatag ctgggatgag accgatgtcc ttagatttag cgtattcaat tagctctgtt
    21241 acttctgcct gtgttagtgc agtaccgttt ggatcgtcgt agtaagcttt agttccttcg
    21301 ataatagctt ttttaacgtc atcactagca taggtttttc cgttggcagt aatggtcata
    21361 tcatcgagta gaaagcgaag tccgtcattt cctagaagga gatggacatc agaatatccg
    21421 agctcactgg ccttgtctac gatgcgtttg agctggttca gagtaaagta tttgcgtcca
    21481 gcatcgattg agattacctt gtttttggca agtttttcaa cctcacgttt agcttcttct
    21541 tctttttgag cttcaggcgt gagggtcaag ttgttgacag tttcttgaag tttagcaatg
    21601 gcttgatcaa tcgtatcttg ttgggcacgg ctaaggttgc tatcgagaga gcgaatagct
    21661 ttttcagctt cttttacggc cgtgacgctt tctgcagtat aacggttcag gtcttttggt
    21721 acctcgttaa gtgcttgctc tgcagattca taatcagctg cgaagtattc agcgttggca
    21781 tttgcaaaat gacgcatgag tttgaagagg cgtgatggtg aataacgtgc agatggagtg
    21841 tcagcccaag cagctaccat accaccgatg attgggatat cagctccttc tgtttttggt
    21901 acagaagtga ttggtgtgtt tttaatacca ttgagcccct gatcgagatt gtaccagcct
    21961 tggccatcag cgtttcgtcc aagaacgtag taccaagcat cattggtatt aaggatttgg
    22021 tgaccttttt cagctagtag tttagaagaa gcgacatcgt agcctcccca accaccagtc
    22081 cacatagaaa cgatgatgtc tttgtcaaaa ctaccaaagc ttgtgtcgct attgtagtag
    22141 ataccgtcgt taaaagccat tggtttgaga ccgtgcgatt ttacaatacg agcgaggtca
    22201 ttggcgtagg caataaattt ttcatagcct tttacagggt agccttcgtt tggatagtat
    22261 ttatcagctt gaagcacact ccaaccttta gcatctgtcg catcattggc atattcatca
    22321 agtccgatgt tgaagatttc agtctttttc gcgaaataag cagcatactt gtcgataagg
    22381 gcttttgtaa aagcgacagc ttgttcgttg tcaagatcga cagtacgggc tgatttcttc
    22441 ccaaaatagc taaagttagg gttttggatt cccaattctt tcatggcatt gagaatcgca
    22501 tccatgtgtc caggactatt tactgtcgga atgagaccga tacctttatc tttggcatag
    22561 ttaatcagat ctgtcatttg actttctgtt aagtgattgc cgtttggatc gttgtaataa
    22621 tcatttgtac ctttttcaat ggcgcgtttg acatcgtcac tggcataggt cttgccgtta
    22681 gctgtgatgc tcatatcgtc caacatgaaa cggagtccat catttccgac taataggtgt
    22741 aaatcagtgt agccataatg tttcgcttta tcgatgattt ccttgagctg ttctggtgag
    22801 aaatatttac gtccagcatc aatagaaaca attttctttt tcgctagttt ttcatttaca
    22861 gttgcagcac gttcctttcc tgcctctgtt gccggtttgt cagcctctgc tttcgcttca
    22921 tcttttttag ctggtttatc cttgtcagtc ttgtctgtat ttgactcttt agaatcaacc
    22981 tctttcgctt cttccttttt agggctagct tcttctgcct ttttattagc agtttctttt
    23041 tcagcagaag ttggagttac cacttctgct ttatcactag gagttgaact aacttcctct
    23101 tgtggttttt cttctgtttt tggaagacta gctaccttat cagtagctgg agtttctgtt
    23161 tctacagttt ttggagcttc tggttgaagc actgctttag gtgtttcctc agtccgattt
    23221 tcggatgatt gaggggaatc agaaaccgta tggatggtcg gttggttttc tgtagtagta
    23281 ggagtaactc catcggctgc aacagtctgt gcttggaagg caaatccaat tagaacagaa
    23341 gctgctccta cagcgtattt acgaatagaa aaacgctgtt gtttttcatg tttcattgca
    23401 aaacctcctg attgcattgt tatattgata gcgattatat aaatcaacgc ctttatttta
    23461 tttcttatat taatttctta tattaacgag agtcaagagg agatgacaaa aaactataat
    23521 aagtataaaa aaatataaaa tttaaactta agatttcaga ttggtcggaa aaaatacgta
    23581 tatatatcta gtataatttt tggttctatt tctataaaat attccacaaa ttatagaatt
    23641 ttccaaaaat aggtaagcgc tacctttttg gtgtagtata ataagcatag aaaaagccca
    23701 agcgattagc tcaggttttc ttcttagtga tcacggtcac atgagataaa tttaatcttg
    23761 tagtaatcag atcgtttgta agtttcactg tattctaaaa cttggccagt tgattcgagt
    23821 ttggtgattt tagtttgtag gacagtaggg aattgttcat cgactccgag gactgaagct
    23881 gcatgttctg gagttggaaa gactatttcg ttgatttctt caaagtgttc atcattcatg
    23941 tgaatgtggt agtctaactt gaaacgatta tagatagaac tatagtattc aaggtttgga
    24001 taatttgcgt tgatatattg ttctgggatg taggatgtat ggtagatata aacgacaccg
    24061 tttgattcgc ggatacgttc aatcttgtag tagaattgat cgccgcgtag acccaatttt
    24121 tccaagtaaa caagcttgtt tccgcgttca attgaaagaa cagttacctt atcatcttta
    24181 gcattgaaga gttcaatatc tgaaaactct acaagcttgt gtttgcgtgc acgtgaaacg
    24241 aaggttcctt ttccttgttg gcggacaata tagccatctt tggcaaggtc gtttaaggcg
    24301 cgaacaactg tgatagagct gacatcgtac attgaaatga gttctgcttc agtgtaaaat
    24361 ttatctccac tgctaaactg cccagagatg attttatttt ttaattcgtc ttttatgtat
    24421 tgatatttgg gaattgccat atttttacct ctattttcct tcttccatag aaaattttac
    24481 cataaaagcg aaaaaaatag tagtcttttg aataaaaaat tagaataata gtttgaaaaa
    24541 ttaagcgata tagggattgc tcatatttta taaatgtatt ctatacaaaa ttattttagg
    24601 tcctatattc cgaatttaaa gagcgtttga aatgggtcta aaaatgtttg aaactaattg
    24661 aatgtgtgta taatagtaaa aatgcagtaa atatcccgac aatttctatt gacaattcaa
    24721 acagattggt ttataattaa tataacaaca aatgaaagcg caaactttcg cggtcggaag
    24781 gtagttttat gacacgattt gagatacgag atgatttcta tctcgatgga aaatcattta
    24841 agattttatc tggtgccatt cattatttta gggttcctcc agaggattgg tatcattcgc
    24901 tctataactt gaaggctctt ggttttaata cggtagagac ttatgttgct tggaatttac
    24961 acgagccttg tgaaggtgag tttcattttg aaggtgatct ggatttagag aaatttctcc
    25021 aaatagcgca ggatttgggt ctctacgcaa ttgtgcgtcc gtctccattt atctgtgcgg
    25081 aatgggaatt cggtggctta ccagcttggc tcttgaccaa gaacatgcga attcgctcat
    25141 ccgacccagc atatatcgag gcagttggtc gctactatga tcagttattg ccaagactgg
    25201 tgcctcgttt gttggacaat ggtggcaata ttctcatgat gcaggttgaa aatgagtatg
    25261 gttcttacgg agaagataag gcttacctga gagcgattcg acagctaatg gaagagtgtg
    25321 gcgtaacctg tcccctcttt acatcagatg gtccatggcg agctactctg aaagctggaa
    25381 ccttaattga agaggacctc tttgtaacag gaaactttgg ttctaaggca ccttacaact
    25441 tttcgcagat gcaggaattc tttgatgaac atggtaagaa atggccactc atgtgtatgg
    25501 agttctggga tggttggttc aatcgctgga aagaaccgat tatcacacgg gatcctaagg
    25561 aattggcaga tgcagttcga gaggttttgg aacaaggctc tatcaatctt tacatgttcc
    25621 acggtggtac aaactttggt ttcatgaatg gttgctcagc tcgaggaact ttggacctgc
    25681 cacaagttac gtcttatgat tacgatgccc ttctggatga agaaggaaat ccaactgcta
    25741 aatatcttgc agtcaagaag atgatggcaa cacatttttc agagtatccg cagttggaac
    25801 cactctacaa agagagtatg gagttggatg ctattccact agttgaaaaa gtttctttgt
    25861 ttgaaacctt agatagcttg tcaagtcctg tagaaagtct ctatcctcaa aagatggagg
    25921 agctgggaca aagttatggc tacctacttt atcgaacaga aacaaactgg gatgcagaag
    25981 aagaaagact tcgtatcatt gatggtcgag atagggccca gctgtatgtc gatggtcagt
    26041 gggttaaaac tcaatatcag acagagattg gggaagatat tttttatcaa ggtaaaaaga
    26101 aagggctatc taggttagat atcttgatag aaaatatggg gcgtgtcaac tatgggcata
    26161 agttcttagc ggatacgcaa cgtaagggaa ttcggacagg ggtctgtaag gatctgcatt
    26221 tcttactaaa ctggaaacac tatccactcc cactagacaa tcctgagaaa attgattttt
    26281 caaaaggatg gactcaagga caaccagcct tttacgctta tgactttaca gtcgaagagc
    26341 caaaagatac ttacctagac ttgtctgagt ttggtaaggg ggttgccttt gtcaatgggc
    26401 agaatctagg acgtttttgg aacgttggcc caactctctc actttatatc cctcatagct
    26461 atctcaagga aggtgccaac cgcatcatta tctttgaaac agaaggtcaa tataaagaag
    26521 agattcattt aactcgtaaa cctacactaa aacatataaa gggggaaaac ttatgacaat
    26581 tgtaggatgc cgtattgatg gacgtttgat ccacggacaa gtagccaatc tttgggctgg
    26641 aaaactaaat gtttcacgca ttatggttgt agacgacgaa gttgtcaaca acgatattga
    26701 aaagagtggt ttgaaacttg cgacaccacc aggtgtgaaa ttgagtattt tgccagttga
    26761 gaaagctgca gccaatattc ttggtggcaa atacgatagc caacgtctct ttatcgtggc
    26821 tcgtaaacca gaccgcttcc ttggtttggt agaagcaggt gtaccacttg aaacccttaa
    26881 tgttgggaat atgtctcaaa caccagaaac tcgttctatt acacgttcta tcaacgtagt
    26941 agacaaggat gtggaagact tccacaaact ggcagaaaaa ggtgttaaac ttactgctca
    27001 gatggttcca aatgatccaa tttcagactt tttgagctta ttaaaatagg aaaaaaattt
    27061 ttaggaggtc attgttatga tacaatggtg gcaaatttta cttctcactt tgtactcagc
    27121 ttatcaaatc tgtgatgagt tgacgatcgt ttcatctgca ggttcccctg tatttgctgg
    27181 tttcattact ggtttaatca tgggagatgt gactactggt ttacttatcg gtggtaactt
    27241 gcaactgttc gttcttgggg ttggtacctt cggtggtgct tctcgtatcg acgcaacttc
    27301 tggtgcggtt cttgcgacag ccttctctgt ttcacaagga attgatgcac cgcttgccat
    27361 tactacaatc gctgtaccag tagcagctct cttgacttac ttcgacgttc ttggtcgtat
    27421 gactactacc ttcttcgctc accgtgtgga tgctgcaatc gaacgctttg actataaagg
    27481 tattgaacgc aactacttgc ttggtgcgat tccgtgggct ctatctcgtg cccttccagt
    27541 cttctttgcc cttgcttttg gtggtgcctt tgtacaatca gtagtagact tcgttgaagc
    27601 ctacaaatgg gttgcagatg gcttgacact tgcaggacgt atgcttccag gtcttggatt
    27661 tgcaatcttg cttcgttacc ttccagttaa acgtaacctt cactaccttg ctatgggatt
    27721 tggtttgaca gctatgttga ctgttcttta ctcatatgta acaggtcttg gtggcgctgt
    27781 tgctggtatc gtaggtactc ttcctgctga agttgctgaa aaaattggtt tcgtgaacaa
    27841 cttcaaaggt ttgtctatga ttggtatttc tatcgtaggt attttccttg cagtgcttca
    27901 cttcaaaaat agccaaaaag tagctgtagc agcaccttct acaccatcag aaagtgggga
    27961 aatcgaagat gacgaattct aattacaaac ttacaaaaga agattttaat caaatcaaca
    28021 aacgtagctt gtttactttc caattaggtt ggaactacga acgtatgcaa gcttctggtt
    28081 acctttacat gatcttgcct cagttgcgta aaatgtatgg tgatggaact cctgaattga
    28141 aagaaatgat gaaagttcat actcaattct tcaatacttc accattcttc cataccatta
    28201 tcgctggttt tgaccttgcc atggaagaaa aagatggtgt aggttcaaaa gacgccgtta
    28261 acggtatcaa gacaggtttg atgggaccat tcgctcctct tggggataca atctttggtt
    28321 cacttgtacc tgctatcatg gggtcagtcg cagcaactat ggctatcgct ggccaacctt
    28381 gggggatctt cctttggatt gcagttgcag tagcgtatga catcttccgt tggaaacagt
    28441 tggaatttgc ttacaaagaa ggggttaacc ttatcaacaa catgcaaagt accttgacag
    28501 ctttgattga cgctgcatct gtacttggtg tcttcatgat gggtgctctt gtagcaacag
    28561 tgattaactt tgaaatttct tacaagttgc caatcggtga aaagatgatt gatttccaag
    28621 acatcttgaa ccaaatcttc ccacgtttgc ttccagcaat ctttactgcc tttatcttct
    28681 ggttgcttgg taagaaaggt atgaactcta ctaaagctat cggtattatt atcgtacttg
    28741 ctttggctct ttctgccctt ggtcactttg cacttggaat gtaattcctt atgactaaat
    28801 cattaatttt ggtgagccat ggtcgcttct gtgaggagct tagaggtagc acagaaatga
    28861 ttatgggccc acaagacaac atttacacag tagctcttct tccagaagat ggcccagaag
    28921 aatttactgc taaatttgaa gctgttattg aaggattgga tgatttccta gtctttgcgg
    28981 atcttctcgg tgggacacct tgtaatgtgg tgagtcgctt gatcatggaa ggtcgtgata
    29041 ttgaccttta cgcagggatg aatcttccaa tggtgattga atttatcaat gcgagcctta
    29101 caggcgcaga tgcggactac aagagccgtg ctgcagaaag cattgtgaaa gttaatgacc
    29161 tgttagcggg cttcgatgat gacgaagatg aataatactc ttcgaaaatc tcttcaaact
    29221 acgtcaacgt cgccttgccg taggtatatg ttactgactt cgtcagtctt atccggcaac
    29281 ctcaaaacgg tgttttgagc tgacttcgtc agtcttatcc ggcaacctca aagcagtgct
    29341 ttgagcagcc tgcggctagt ttcctacaga ttttagttgg aactcgattc aattcatgtg
    29401 acaacgtgaa aatcgttaga gcattttata tagaatatac atgggaatgt agcttactcc
    29461 cattcccata tttaatagaa aaagaggaac tcaatgctac attatacaaa agaagacttg
    29521 ctcgaattgg gtgcagaaat cactacgcgt gaaatctacc aacagcctga tgtatggaga
    29581 gaagcttttg aattttatca agcaaaacgt gaagaaattg cagccttcct acaagaaatc
    29641 gctgataaac atgactatat taaagttatc ttgacaggtg ctgggacttc tgcttatgtg
    29701 ggagatacct tgctacctta ttttaaggaa gtctatgacg aacgcaaatg gaatttcaat
    29761 gctattgcga caacagatat cgttgccaat ccagcaacct atttgaaaaa agatgtggca
    29821 actgtccttg tgtcttttgc tcgtagtggg aattcgcctg aaagtttggc gactgttgat
    29881 ttggccaaat ccttggtgga tgagctttat caagtgacga ttacttgtgc agcagatggt
    29941 aaattggctc ttcaagctca cggtgatgat cgtaatctct tgctcttgca accagctgtc
    30001 tctaatgatg ctggatttgc catgacttct agctttacgt ctatgatgtt gacaactctc
    30061 ttggtctttg atcctacaga atttgctgtt aagtctgaac gttttgaagt tgtatctagt
    30121 cttgcccgta aagttttaga caaggcagaa gatgtcaaag agctcgttga tttagacttt
    30181 aaccgtgtca tctatctagg cgctggtcct ttctttggac ttgctcatga agctcagctc
    30241 aagattttgg aattaactgc tggtcaagtt gcgaccatgt atgaaagccc agttggcttc
    30301 cgtcacggtc caaaatctct tatcaacgac aatacagttg ttttggtctt tggtacaacg
    30361 acagactaca ctcgtaagta cgacttggac ttggttcgtg aagttgctgg tgaccagatt
    30421 gctcgtcgtg ttgtgctttt gagtgatcaa gcttttggtc ttgaaaatgt caaagaagtg
    30481 gcccttggtt gtggcggtgt cttgaatgat atttaccgtg tcttccctta catcgtttat
    30541 gcccaactct ttgctttatt gacttcactc aaggtagaaa ataaaccaga tacaccgtct
    30601 cctacaggta cagtaaaccg tgtagtacaa ggtgtcataa ttcacgaata tcaaaagtaa
    30661 gacagtgttt atgaattctt gacaagagga tttgtaaatt atcagataaa ccatagattg
    30721 tcagtacgct ttctatggtt tgtttgcttg agagaaatag taaaaggaga acagaatgaa
    30781 agcatacaca gagcgtgtat ttggaaatgt tgagggtgag gatgtcttgg cctatcgatt
    30841 tgagacagac ggtggctacc aacttgaggt tatgacttat ggtgcgacta tcttgcgcta
    30901 tgtcgcacct gacaaggctg gaaattttgc caatgttatc ttgggatttg atgactttga
    30961 tagttatgta ggcaatagtc ccaagcatgg agcaagtgta ggtcctgtag cgggtcgtat
    31021 tgcaggtgcg acctttgagc tcaatggtaa gacctatgac cttgaggtta ataatgctag
    31081 caactgtaat cacagtggtt caactggttg ggattccagc ttgtttgaag ttgaagaagt
    31141 aagcgatcat ggcttgactc tctacacaga gcgtacagat gggacaggag ggttccctgg
    31201 aaatctcaag atttggatca gttatcactt ggaagaaact ggtgcctatg aaatcagcta
    31261 caaggtaacg accgatcagg atacgctggt caatccaacc aaccacagct atttcaactt
    31321 gtctggtgat ttcacgcaga cgattgaccg tcatgtcttc caactaaaca cagagggcat
    31381 ttactcaatc gctcctgacg gtgttcctgc caaaactcca gaagccaacc gtgatgtggt
    31441 caaacacgtc tacaatggta ccttgttgaa ggatatcttt gcagaagaag atgagcaaat
    31501 ccagctggca tcaggtttgg atcatccatt tgcccttcct gcaggccatg acaatgctgg
    31561 attcctttat gaccaaaatt caggtcgctt cctgcttttc aagacagaag ctccttgctt
    31621 tgtggtctac acagcaaact ttgtggatga aagtgtcatc ataggaggtc agccaatgct
    31681 acagcacaat gggattgctc ttgaagcgca agctttacca gatgccattc acagtgacct
    31741 taaaggccaa gtcattctta aagctggtca aaccttcacc agtaagacac gttatgaact
    31801 tgttgtgaag taaaagagtc attgcgccta cttttgggag ctaggaatag gtacgcagag
    31861 acaaatagta ggaaaatatg atataactaa gcgttgaaag ctatctgtta atataatatt
    31921 caaactacaa taaggagtaa gaaagaaacg aagaaaattg tatttgctag tgccttggct
    31981 ttgaccttgg ctggagcagt tttgacaaat gatgtttttg cgaacgacag acttgtggca
    32041 acacaaacta ctgatggtaa aaatgaaaat gtattgacct cagaggtgct aaaaccttct
    32101 agtggcaatg ttttggttgg aatcaaagga gaatttgtgg ctcctcatca acaatctatt
    32161 ttggatgcca tcaatgctat ctgtaaagaa gcggctgacg aaggtttggt agataagtat
    32221 gtccctatca aatgatcaac tgacctagaa aaggcagctt ttgccagagc tacagaagca
    32281 tctataacca tggatcatac ccgtctttct agcaaagatc tttggagtgc ctttccaact
    32341 tctaatagta taatgggaga aaatttggca tggaatcatg acggttttct aaaagctatt
    32401 gaacaatggc gtgctgaaaa agcagattat gtggagaaaa aaatagtggt tcagacaacg
    32461 ggaaatctgg tcactatgag tcgctaatta accctaaatt tacacacatg gggatggcag
    32521 cttttaaaaa tcctaacaat caatacaaag ctattacaat tgctcaaact ctaggtgatg
    32581 atgcttcttc agaggaattg gctggtagat atggttctgc tgttcagtgt acagaagtga
    32641 ctgcctcaaa cctttcaaca gttaaaacta aagctacggt tgtagaaaaa ccactgaaag
    32701 attttagagc gtctacgtct gatcagtctg gttgggtgga atctaatggt aaatggtatt
    32761 tctatgagtc tggtgatgtg aagacaggtt gggtgaaaac agatggtaaa tggtactatt
    32821 tgaatgactt aggtgtcatg cagactggat ttgtaaaatt ttctggtagc tggtattact
    32881 tgagcaattc aggtgctatg tttacaggct ggggaacaga tggtagcaga tggttctact
    32941 ttgacggctc aggagctatg aagacaggct ggtacaagga aaatggcact tggtattacc
    33001 ttgacgaagc aggtatcatg aagacaggtt ggtttaaagt cggaccacac tggtactatg
    33061 cctacggttc aggagctttg gctgtgagca caacaacacc agatggttac cgtgtaaatg
    33121 gtaatggtga atgggtaaac taggctcagg ccataggtaa agcattcatc ttacttagca
    33181 aaaagaatga acgataagaa agaggttgat ggcgaacatt ggcctctttt gatttataaa
    33241 gattggattc ttgtcgcctc aatttcagac ttttctattg taagctaata ttttatagcc
    33301 cattaaaagc ataagcggta atctaattta aaaaatgctg taattagtct gaagtccaca
    33361 cttacttgtt gagatgttat ctctgttttt tatcgttata atttactgta ttttttatag
    33421 tatgcagaat atttttaagt atatttcaat agaaatttct atcgatttat tgtataatga
    33481 taagtaattg ttgaaaagta ctcagaaaat tccatactat attattttta tgtttatact
    33541 tttatgctat aaaatataga ttgatataaa gaatatagaa aaagcgaggt taatatgagc
    33601 cgaaaaagca ttggtgagaa acgccatagt ttctcgatga gaaagttgtc agtgggattg
    33661 gtatcagtta ctgtatctag tttctttttg atgagtcaag ggattcaatc ggtatcggcc
    33721 gataatatgg aaagtccaat tcattataag tatatgaccg agggtaaatt gacagacgag
    33781 gaaaaatcct tgctggtaga ggcccttcca caactggctg aagaatcaga tgatacttat
    33841 tacttggttt atagatctca acagttttta ccgaatacag gttttaaccc aactgttggt
    33901 actttccttt ttactgcagg attgagcttg ttagttttat tggtttctaa aagggaaaat
    33961 ggaaagaaac gacttgttca ttttctgctg ttgactagca tgggagttca attgttgccg
    34021 gccagtgctt ttgggttgac cagccagatt ttatctgcct ataatagtca gctttctatc
    34081 ggagtcgggg aacatttacc agagcctctg aaaatcgaag gttatcaata tattggttat
    34141 atcaaaacta agaaacagga taatacagag ctttcaagga cagttgatgg gaaatactct
    34201 gctcaaagag atagtcaacc aaactctaca aaaacatcag atgtagttca ttcagctgat
    34261 ttagaatgga accaaggaca ggggaaggtt agtttacaag gtgaagcatc aggggatgat
    34321 ggactttcag aaaaatcttc tatagcagca gacaatctat cttctaatga ttcattcgca
    34381 agtcaagttg agcagaatcc ggatcacaaa ggagaatctg tagttcgacc aacagtgcca
    34441 gaacaaggaa atcctgtgtc tgctacaacg gtgcagagtg cggaagagga agtattggcg
    34501 acgacaaatg atcgaccaga gtataaactt ccattggaaa ccaaaggcac gcaagaaccc
    34561 ggtcatgagg gtgaagccgc agtccgtgaa gacttaccag tctacactaa gccactagaa
    34621 accaaaggta cacaaggacc cggacatgaa ggtgaagctg cagttcgcga ggaagaacca
    34681 gcttacacag aaccgttagc aacgaaaggc acgcaagagc caggtcatga gggcaaagct
    34741 acagtccgcg aagagactct agagtacacg gaaccggtag cgacaaaagg cacacaagaa
    34801 cccgaacatg agggcgaagc ggcagtagaa gaagaacttc cggctttaga ggtcactaca
    34861 cgaaatagaa cggaaatcca gaatattcct tatacaacag aagaaattca ggatccaaca
    34921 cttctgaaaa atcgtcgtaa gattgaacga caagggcaag cagggacacg tacaattcaa
    34981 tatgaagact acatcgtaaa tggtaatgtc gtagaaacta aagaagtgtc acgaactgaa
    35041 gtagctccgg tcaacgaagt cgttaaagta ggaacacttg tgaaagttaa acctacagta
    35101 gaaattacaa acttaacaaa agttgagaac aaaaaatcta taactgtaag ttataactta
    35161 atagacacta cctcagcata tgtttctgca aaaacgcaag ttttccatgg agacaagcta
    35221 gttaaagagg tggatataga aaatcctgcc aaagagcaag taatatcagg tttagattac
    35281 tacacaccgt atacagttaa aacacaccta acttataatt tgggtgaaaa taatgaggaa
    35341 aatactgaaa catcaactca agatttccaa ttagagtata agaaaataga gattaaagat
    35401 attgattcag tagaattata cggtaaagaa aatgatcgtt atcgtagata tttaagtcta
    35461 agtgaagcgc cgactgatac ggctaaatac tttgtaaaag tgaaatcaga tcgcttcaaa
    35521 gaaatgtacc tacctgtaaa atctattaca gaaaatacgg atggaacgta taaagtgacg
    35581 gtagccgttg atcaacttgt cgaagaaggt acagacggtt acaaagatga ttacacattt
    35641 actgtagcta aatctaaagc agagcaacca ggagtttaca catcctttaa acagctggta
    35701 acagccatgc aaagcaatct gtctggtgtc tatacattgg cttcagatat gaccgcagat
    35761 gaggtgagct taggcgataa gcagacaagt tatctcacag gtgcatttac agggagcttg
    35821 atcggttctg atggaacaaa atcgtatgcc atttatgatt tgaagaaacc attatttgat
    35881 acattaaatg gtgctacagt tagagatttg gatattaaaa ctgtttctgc tgatagtaaa
    35941 gaaaatgtcg cagcgctggc gaaggcagcg aatagcgcga atattaataa tgttgcagta
    36001 gaaggaaaaa tctcaggtgc gaaatctgtt gcgggattag tagcgagcgc aacaaataca
    36061 gtgatagaaa acagctcgtt tacagggaaa cttatcgcaa atcaccagga cagtaataaa
    36121 aatgatactg gaggaatagt aggtaatata acaggaaata gttcgagagt taataaagtt
    36181 agggtagatg ccttaatctc tactaatgca cgcaataata accaaacagc tggagggata
    36241 gtaggtagat tagaaaatgg tgcattgata tctaattcgg ttgctactgg agaaatacga
    36301 aatggtcaag gatattctag agtcggagga atagtaggat ctacgtggca aaacggtcga
    36361 gtaaataatg ttgtgagtaa cgtagatgtt ggagatggtt atgttatcac cggtgatcaa
    36421 tacgcagcag cagatgtgaa aaatgcaagt acatcagttg ataatagaaa agcagacaga
    36481 ttcgctacaa aattatcaaa agaccaaata gacgcgaaag ttgctgatta tggaatcaca
    36541 gtaactcttg atgatactgg gcaagattta aaacgtaatc taagagaagt tgattataca
    36601 agactaaata aagcagaagc tgaaagaaaa gtagcttata gcaacataga aaaactgatg
    36661 ccattctaca ataaagacct agtagttcac tatggtaaca aagtagcgac aacagataaa
    36721 ctttacacta cagaattgtt agatgttgtg ccgatgaaag atgatgaagt agtaacggat
    36781 attaataata agaaaaattc aataaataaa gttatgttac atttcaaaga taatacagta
    36841 gaatacctag atgtaacatt caaagaaaac ttcataaaca gtcaagtaat cgaatacaat
    36901 gttacaggaa aagaatatat attcacacca gaagcatttg tttcagacta tacagcgata
    36961 acgaataacg tactaagcga cttgcaaaat gtaacactta actcagaagc tactaaaaaa
    37021 gtactaggag cagcgaatga tgcagcctta gataacctat acttagatag acaatttgaa
    37081 gaagttaaag ctaatatagc agaacaccta agaaaagtat tagcgatgga taaatcaatc
    37141 aatactacag gagacggtgt agttgaatac gtaagtgaga aaatcaaaaa taacaaagaa
    37201 gcatttatgc taggtcttac ttatatgaac cgttggtacg atattaatta tggtaaaatg
    37261 aatacaaaag atttatctac gtacaagttt gactttaacg gaaataatga gacttcaacg
    37321 ttggatacta ttgtcgcatt aggaaatagt ggactagata acctgagagc ttcaaatact
    37381 gtaggtttat atgcgaataa acttgcatcg gtaaaaggag aagattcagt ctttgacttc
    37441 gtagaagcgt atagaaaact gttcttacca aacaaaacaa ataacgagtg gtttaaagaa
    37501 aatacaaagg catatatagt cgaaatgaag tctgatattg cagaagtacg agaaaaacaa
    37561 gaatcaccaa cagccgatag aaaatattca ttaggagttt acgatagaat atcagcacca
    37621 agttgggggc ataagagtat gttattacca ctactaactt tacctgaaga atctgtgtat
    37681 atttcatcga atatgtctac acttgcattc ggttcgtatg aaagatatcg tgatagtgtg
    37741 gatggagtta ttctttcagg agatgcttta cgaacttatg taagaaatag agttgatata
    37801 gcagcgaaaa ggcatagaga ccattatgat atttggtaca atcttcttga cagtgcttca
    37861 aaagaaaaac ttttccgttc tgtgatagtt tatgatggat tcaatgtaaa agatgagaca
    37921 ggaagaactt attgggcaag gttaacggat aaaaacatcg gctctattaa agaattcttc
    37981 ggacctgttg ggaaatggta tgagtataat agtagtgcag gagcgtatgc gaatggaagt
    38041 ttaacgcact ttgtgttaga tagattatta gatgcttatg gaacgtcggt ttatactcat
    38101 gaaatggttc ataattctga ttctgcaatc tactttgaag gaaatggtag acgtgaagga
    38161 ttgggagcgg agttatacgc acttggttta ctgcaatctg tagatagtgt aaattctcat
    38221 attttagctt taaatacgtt atataaagca gaaaaagatg atttgaatag attgcataca
    38281 tataatccgg tggaacgttt cgattcggat gaggcgcttc aaagttatat gcatggatca
    38341 tatgatgtaa tgtatacact tgatgcgatg gaagcaaaag cgatattagc tcaaaataat
    38401 gatgttaaga aaaaatggtt tagaaaaata gaaaattatt acgttcgtga tactagacat
    38461 aataaagata cacatgcagg aaataaagtc cgtccattaa cagatgaaga agtagctaac
    38521 ttaacatcgt taaactcatt aatcgacaac gacatcataa atagacgtag ctatgatgat
    38581 aatagagaat ataaacgaaa tggctactat actataagta tgttctctcc tgtatacgca
    38641 gcgctaagca attcgaaagg tgctcctgga gatattatgt ttagaaaaat agcttatgaa
    38701 ttacttgcgg aaaaaggtta tcacaaagga ttcctacctt atgtttctaa tcagtacgga
    38761 gcagaagcat ttgccagcgg aagcaaaaca ttctcatcat ggcatggaag agatgttgct
    38821 ttagtgacag atgatttagt atttaagaaa gtattcaatg gtgagtactc atcatgggct
    38881 gatttcaaaa aagcaatgtt taaacaacgt atagataaac aagataatct gaaaccaata
    38941 acaattcaat acgaattagg taatcctaat agtacaaaag aagtaactat aacaacggct
    39001 gcacaaatgc aacaattaat taatgaagcg gctgcgaaag atattactaa tatagatcgt
    39061 gcaacgagtc ataccccagc aagttgggtg catttattaa aacaaaaaat ctataatgca
    39121 tatcttcgca ctacagatga ctttagaaat tctatatata aataagattg tagagtttca
    39181 ttgttgagta gtgtttcttg taaggatgag gagtcagatg acaaatcgac tcctttttct
    39241 tatggatcga tgtagagatt tgattgaatg cagattgcag gaatcatctt caactcatca
    39301 acgaccaatg gtgacaaggt ggatttcaat cccacagaaa atgttgattt gagaaataac
    39361 tttgctagtc tagtaaaata aatacaaaac aatcctagaa gattttttct gggattgttt
    39421 tttgctgagt gggatgcttc aagttgtctg gcttgacttt cttgagggaa gttatataat
    39481 agttgtaata attaaagtta caataaaaga ggaaatattt atgacatatg aataccagag
    39541 ccacatttat ttagcagagg cagttttaaa tgtgaaggat ttggtcagtc aaacagtttt
    39601 ttatcagcag attattggtt tagaaatcct atctcaaacg gatacagagg tcgttctggg
    39661 acttggagga aaagccttgg tacacttgat tcaagcacaa gagggtggag aagtaaggga
    39721 acattatggt ctttaccatc tggctattct tttgccgaca cgaaaggctt tggcggatgt
    39781 cttgaagcac ctgacggatt tacagattcc tcttgttggc ggtgcagatc acggttacag
    39841 tgaggccctt tacttagagg acttggaggg aaatggcatt gaactctatc gagataagcc
    39901 agtttccaca tgggatattc gagaagatgg acgtattatc ggggtgactg aagtccttgc
    39961 ggctcaggat atctatgagt tgggggaaag agtagagcct tttatcctag cagagggtac
    40021 gagaatgggg catattcatc tttctgtcaa ggatagtcga aagtccagac agttttatca
    40081 aacggtgtta gggctcgagg ataaattcag tgtgcctagt gctagttgga tcgcagctgg
    40141 ggactaccat catcatttag cagtcaacga atggggagga aaaggtctgg atccgcgtaa
    40201 acaagtccta ccaggtttag cctactatgt catcgaagtc gcacataaag aagaactgtt
    40261 aacgattgcc caacgagcac aagaagttga cgcaccaatc aaatggatga catcgatcca
    40321 attggaaatc acagactcag atggcatcgt gacccgtatt cgtttagcta gatagatggt
    40381 atgtgatgaa ggtagagcat caattgtaga aagtgctcct tattctagtc agatacgtgg
    40441 aaaatttgat atgatagtag ggaataagga gaagaataat atgactagcg aataccagaa
    40501 aatgatagca ggagagtttt accgtccatc agacccagaa ctgagagcct tagctcaggc
    40561 ttctcgccaa aaacaggccg cctttaacaa ggaagagaac cccttgaagg gagccgaaat
    40621 catcaagact tggtttgcct caaccgggaa aaatctttac atcaacactc gcttgatggt
    40681 ggactacggt gtcaacatcc atctagggga aaatttttat tctaattgga acttgaccat
    40741 gctggatatc tgtcccattc gtatcgggga caatgctatg attggtccta attgtcagtt
    40801 tttgacaccc ctccatccac tagatccaca ggaacgcaat tcaggtatcg agtacggaaa
    40861 gcctatcaca atcggagata atttctggac tggtggtggc gtcattgtcc ttcctggagt
    40921 gacactggga aataatgtcg ttgcaggagc aggggcagta attaccaaat cttttggcga
    40981 caacgttgtc ctagctggca atcctgcgcg cgtgattaag gaaatacctg ttaaatagaa
    41041 gtaaaaagga acagctgggg ttgtttcttt tttgtaggtt tcatcatttt ttacccagtt
    41101 cacatttacc tactctatct cttagcaagt ctgtttcatt aagcaagttc aaagcatctc
    41161 gtaagtggga tgtttttctc ctcagttcat cagcttcctc cttgacactc ggtcagattt
    41221 tgatacaata gtacaaaatt agaggaggca ggctatgatt cagaaacatg cgattcctat
    41281 tttagagttt gatgacaatc ctcaggcggt tatcatgccc aatcacgagg ggctggactt
    41341 gcagttgcca aagaagtgtg tttatgcatt tttaggtgag gagattgacc gctatgcgag
    41401 ggaagtaggg gcgaactgtg ttggcgaatt tgtttctgcc accaagacct atccagttta
    41461 tgtcgtgaac tacaaggacg aggaggtctg tctggctcag gctcctgttg gctccgctcc
    41521 agcagcccag tttatggatt ggttgattgg ctatggtgtg gagcagatta tctctactgg
    41581 gacctgtggt gtcctagctg atatagagga aaatgccttt ctagtccctg ttcgcgctct
    41641 gcgagatgaa ggagccagtt accactatgt ggcaccttgt cgttatatgg aaatgcagcc
    41701 agaggctatt gctgctattg aggaagtttt ggaagacaga gggattcctt atgaagaagt
    41761 catgacctgg acgacagacg gtttttaccg agaaacggct gaaaaggtgg cttatcgtaa
    41821 ggaagaaggc tgtgctgttg tggagatgga gtgttctgct cttgcggcag tagctcaatt
    41881 gcgtggggtt ctctggggtg aattgttgtt cacagcagat tctctagcgg acttggacca
    41941 gtacgacagt cgtgactggg gctcggaagc ttttaataag gcgctagaac tgagtttagc
    42001 aagtgttcac cacctttagt tgtactggca aaggatttgt tttatcataa aatgtctagc
    42061 tcatactttt caaaaatatg tttaaacgag gtcaccttcc tcttgtccta ggcatgttga
    42121 ggttgggaaa aatctttaaa atcagaaaaa cgtatcatat caggtgatga aaactttgac
    42181 actatgcgtt ttatgtcgat aagatttaga gtgagatgaa atgatactct tcgaaaatct
    42241 cttcaaacca ggtcagcttc accttgccgt aggtatatgt tactgacttc gtcagtctta
    42301 tccggcaacc tcaaaacggt gttttgagct gacttcgtca gttctatttg caacctcaaa
    42361 acagtgtttt gagcaacctg tgactagctt tctaatcgat gccttggttt tcattgccta
    42421 taatcaaaaa gagaaatttt ctcctgaaaa gcatatagag tagctggcgt taaaagctcc
    42481 tgtcttgctt ttttgaccta tagtcacatc tatcaagtat tgttcttgcc taagctatca
    42541 ataaaaaggt ggcatttttt aggcttggtg ttagtagatt ttgccttatc ctatctaagt
    42601 catttcgaac tttttatggt acaatggaaa catgttattc aaattatcta aggaaaaaat
    42661 agagctaggc ttatctcgtt tatcgccagc ccgtcgtatt tttttgagtt ttgccttggt
    42721 cattttacta ggctctcttc ttttgagctt gccctttgtc caagttgaaa gctcacgagc
    42781 gacttatttt gatcatcttt tcactgctgt ctctgcagtc tgtgtgacgg gtctctcaac
    42841 ccttccagta gctcacacct ataatatctg gggtcaaata atctgtttgc tcttgattca
    42901 gatcggtggt ctagggctca tgacctttat tggggttttc tatatccaga gcaagcaaaa
    42961 gcttagtctt cgtagccgtg caactattca ggatagtttt agttatggag aaactcgatc
    43021 tttgagaaag tttgtctatt ctatttttct cacgaccttt ttggttgaga gcttgggagc
    43081 tattttgctt agttttcgcc ttattcctca acttggctgg ggacgtggtc tttttagttc
    43141 catttttcta gcgatctcag ccttctgtaa tgccggtttt gataatttag ggagcaccag
    43201 tttatttgct tttcagaccg atttactggt caatctggtg attgcaggct tgattattac
    43261 aggcggcctt ggttttatgg tctggtttga tttggctggt catgtaggaa gaaagaaaaa
    43321 aggacgtctg cactttcata cgaagcttgt actattattg actataggtt tgttgttatt
    43381 tggaacagca actactctct ttcttgagtg gaacaatgct ggaacgattg gcaatctccc
    43441 tgttgccgat aaggttttag ttagcttttt tcaaacagtg acgatgcgaa cagctggctt
    43501 ttctacgata gattatactc aggctcatcc tgtgactctt ttgatttata tcttacagat
    43561 gtttctaggt ggggcacctg gaggaacagc tgggggactc aagattacga cattttttgt
    43621 cctcttggtc tttgcacgaa gtgagcttct aggcttgcct catgccaatg ttgcgagacg
    43681 aacgatcgcg ccgcgaacgg ttcaaaaatc ctttagtgtc tttattatct ttttgatgag
    43741 cttcttgata ggattgattc tgctagggat aacagccaaa ggcaatcctc cctttatcca
    43801 cctcgtattt gaaaccattt cagctcttag tacagttggt gtaacggcaa atctgactcc
    43861 tgaccttggg aaattggctc tcagtgttat catgccactt atgtttatgg gacgaattgg
    43921 tcccttgacc ttgtttgtta gcttggcaga ttaccatcca gaaaagaaag atatgattca
    43981 ctatatgaaa gcagatatta gtattggtta agaaaggaaa gagcatgtca gatcgtacga
    44041 ttggaatttt gggcttggga atttttggga gcagtgtcct agctgcccta gccaagcagg
    44101 atatgaatat tatcgctatt gatgaccacg cagagcgcat caatcagttt gagccagttt
    44161 tggcgcgtgg agtgattggt gacatcacag atgaagaatt attgagatca gcagggattg
    44221 atacctgcga taccgttgta gtcgcgacag gtgaaaatct ggagtcgagt gtgcttgcgg
    44281 ttatgcactg taagagtttg ggggtaccga ctgttattgc taaggtcaaa agtcagaccg
    44341 ctaagaaagt gctagaaaag attggagctg actcggttat ctcgccagag tatgaaatgg
    44401 ggcagtctct agcacagacc attcttttcc ataatagtgt tgatgtcttt cagttggata
    44461 aaaatgtgtc tatcgtggag atgaaaattc ctcagtcttg ggcaggtcaa agtctgagta
    44521 aattagacct ccgtggcaaa tacaatctga atattttggg tttccgagag caggaaaatt
    44581 ccccattgga tgttgaattt ggaccagatg acctcttgaa agcagatacc tatattttgg
    44641 cagtcatcaa caaccagtat ttggataccc tagtagcatt gaattcgtaa agagggatga
    44701 cccctctttt ttgatgccta agatggcaaa tagagacaga agccccttgt cttctagtaa
    44761 aagttcttca aaggctggac tttatggtaa aatagaaaga agtgacaaga gagagtaata
    44821 ctcaatgaaa atcaaagatc aaactaggaa actagctacg ggctgctcaa aacactgttt
    44881 tgaggttgca gatagaactg acgaagtcag taacatctat acggcaaggc gacgttgacg
    44941 cggtttgaag agattttcga agagtataag aaaaaatcag tcccctaaag gagtagatta
    45001 tgaagttatt gtctatcgca atttctagct ataatgcagc agcctatctt cattactgtg
    45061 tggagtcgct agtgattggt ggtgagcaag ttgggatttt gattatcaat gacgggtctc
    45121 aggatcagac tcaggaaatc gctgagtgtt tagctagcaa gtatcctaat atcgttagag
    45181 ccatctatca ggaaaataaa tgccatggcg gtgcggtcaa tcgtggcttg gtagaggctt
    45241 ctgggcgcta ttttaaagta gttgacagtg atgactgggt ggatcctcgt gcctacttga
    45301 aaattcttga aaccttgcag gaacttgaga gcaaaggtca agaggtggat gtctttgtga
    45361 ccaattttgt ctatgaaaag gaagggcagt ctcgtaagaa gagtatgagt tacgattcag
    45421 tcttgcctgt tcggcagatt tttggctggg accaggtcgg aaatttctcc aaaggccagt
    45481 ataccatgat gcactcgctg atttatcgga cagatttgtt gcgtgctagc cagttctaac
    45541 tgcctgaaca tactttttat gtcgataatc tctttgtctt tacgcccctt cagcaggtca
    45601 agaccatgta ctatctgcct gtcgatttct atcgttattt gattgggcgt gaggaccagt
    45661 ctgtcaatga gcaagtgatg attaagtgca ttgaccagca actcaaggtc aatcgactct
    45721 tgatagacca acttgatttg tcccaagtga gtcatcccaa aatgcgagaa tatctgctga
    45781 atcatattga actcacgacg gtgatttcca gtaccctgct caaccgatct ggaacagcgg
    45841 agcatctggc aaaaaaacgc caattgtgga cctatattca gcagaaaaat ccagaagtct
    45901 ttcaggctat tcgtaagacc atgttgagcc gtttgaccaa acattctgtc ttgccagatc
    45961 gcaaactgtc caatgtcgtc tatcaaatca ccaaatctgt ttatggattt aattaatata
    46021 agtgttttat aagagggatt taagaaaaat tttaactttt tcttagtcct ttttaatttc
    46081 aggagattat actagagtca tcaaataaag aaagactcta aggagaatcc tatgaaattc
    46141 aatccaaatc aaagatatac tcgttggtct attcgccgtc tcagtgtcgg tgttgcctca
    46201 gttgttgtgg ctagtggctt ctttgtccta gttggtcagc caagttctgt acgtgccgat
    46261 gggctcaatc caaccccagg tcaagtctta cctgaagaga catcgggaac gaaagagggt
    46321 gacttatcag aaaaaccagg agacaccgtt ctcactcaag cgaaacctga gggcgttact
    46381 ggaaatacga attcacttcc gacacctaca gaaagaactg aagtgagcga ggaaacaagc
    46441 ccttctagtc tggatacact ttttgaaaaa gatgaagaag ctcaaaaaaa tccagagcta
    46501 acagatgtct taaaagaaac tgtagataca gctgatgtgg atgggacaca agcaagtcca
    46561 gcagaaacta ctcctgaaca agtaaaaggt ggagtgaaag aaaatacaaa agacagcatc
    46621 gatgttcctg ctgcttatct tgaaaaagct gaagggaaag gtcctttcac tgccggtgta
    46681 aaccaagtaa ttccttatga actattcgct ggtgatggta tgttaactcg tctattacta
    46741 aaagcttcgg ataatgctcc ttggtctgac aatggtactg ctaaaaatcc tgctttacct
    46801 cctcttgaag gattaacaaa agggaaatac ttctatgaag tagacttaaa tggcaatact
    46861 gttggtaaac aaggtcaagc tttaattgat caacttcgcg ctaatggtac tcaaacttat
    46921 aaagctactg ttaaagttta cggaaataaa gacggtaaag ctgacttgac taatctagtt
    46981 gctactaaaa atgtagacat caacatcaat ggattagtt
//
LOCUS       DAVKNL010000012        45552 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVKNL010000012 DAVKNL010000000
VERSION     DAVKNL010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 45552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45552
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.012"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            271..768
                     /locus_tag="VW411_000611"
     CDS             271..768
                     /locus_tag="VW411_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU9793396.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTAWDFTM
                     FEEKS"
     gene            768..2423
                     /gene="dnaX"
                     /locus_tag="VW411_000612"
     CDS             768..2423
                     /gene="dnaX"
                     /locus_tag="VW411_000612"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEU9793397.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGANTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKSEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            2451..2645
                     /locus_tag="VW411_000613"
     CDS             2451..2645
                     /locus_tag="VW411_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEU9793398.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            2795..3565
                     /gene="sufC"
                     /locus_tag="VW411_000614"
     CDS             2795..3565
                     /gene="sufC"
                     /locus_tag="VW411_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEU9793399.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            3593..4855
                     /gene="sufD"
                     /locus_tag="VW411_000615"
     CDS             3593..4855
                     /gene="sufD"
                     /locus_tag="VW411_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEU9793400.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIECVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIVGKNSKISYLERLESRGEGSDKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            4866..6092
                     /locus_tag="VW411_000616"
     CDS             4866..6092
                     /locus_tag="VW411_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9793401.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQFASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            6079..6519
                     /locus_tag="VW411_000617"
     CDS             6079..6519
                     /locus_tag="VW411_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEU9793402.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQDQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            6573..7985
                     /gene="sufB"
                     /locus_tag="VW411_000618"
     CDS             6573..7985
                     /gene="sufB"
                     /locus_tag="VW411_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEU9793403.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVYYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(8216..9457)
                     /locus_tag="VW411_000619"
     CDS             complement(8216..9457)
                     /locus_tag="VW411_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEU9793404.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            9560..10669
                     /locus_tag="VW411_000620"
     CDS             9560..10669
                     /locus_tag="VW411_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU9793405.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFFYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSHLYENHKIMKERERELAK"
     gene            11203..11550
                     /gene="ssrA"
                     /locus_tag="VW411_000621"
     tmRNA           11203..11550
                     /gene="ssrA"
                     /locus_tag="VW411_000621"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(11733..11820)
                     /locus_tag="VW411_000622"
     tRNA            complement(11733..11820)
                     /locus_tag="VW411_000622"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11784..11786),aa:Ser,seq:gga)
     gene            11982..12722
                     /locus_tag="VW411_000623"
     CDS             11982..12722
                     /locus_tag="VW411_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9793406.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPYSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGINGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            12719..13630
                     /gene="pfkB"
                     /locus_tag="VW411_000624"
     CDS             12719..13630
                     /gene="pfkB"
                     /locus_tag="VW411_000624"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEU9793407.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVESVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDVVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            13627..15579
                     /locus_tag="VW411_000625"
     CDS             13627..15579
                     /locus_tag="VW411_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9793408.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTDHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGALYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            15743..17968
                     /locus_tag="VW411_000626"
     CDS             15743..17968
                     /locus_tag="VW411_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEU9793409.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFLNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEIAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(18013..18441)
                     /locus_tag="VW411_000627"
     CDS             complement(18013..18441)
                     /locus_tag="VW411_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793410.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTVATIASILGLITASITIMN
                     ALDNNKNIKKEPTTSQTTTIVKEIQKESPKENTSPTKETNTSQEKTQQEETPKSSVKE
                     EKKEDQKTATQDSSTPASSKPATENEKQSNAPTSENKSNQ"
     gene            18579..19721
                     /locus_tag="VW411_000628"
     CDS             18579..19721
                     /locus_tag="VW411_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9793411.1"
                     /translation="MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWLIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRHDTILVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKISTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     NYPDIFVFSDEENFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            19730..20944
                     /gene="thiI"
                     /locus_tag="VW411_000629"
     CDS             19730..20944
                     /gene="thiI"
                     /locus_tag="VW411_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEU9793412.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYTQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKE
                     GMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
                     LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            21079..21903
                     /locus_tag="VW411_000630"
     CDS             21079..21903
                     /locus_tag="VW411_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9793413.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFNSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPGQQFGGKGVEYAEFVMEVVKPFIDETYRTKADCQHTAMIGSSLGGNITQFIGL
                     EYQDQIGCLGVFSSANWLHQEAFNRYFECQKLSPDQRIFIYVGTEEADDTDKTLMDGN
                     IKQAYIDSSLCYYHDLIAGGVHLDNLVLKVQSGAIHSEIPWSENLPDCLRFFAEKW"
     gene            21925..22664
                     /locus_tag="VW411_000631"
                     /pseudo
     CDS             21925..22664
                     /locus_tag="VW411_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            22677..23843
                     /locus_tag="VW411_000632"
     CDS             22677..23843
                     /locus_tag="VW411_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU9793414.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIGRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMEQMIADFGQRQE"
     gene            24351..25844
                     /locus_tag="VW411_000633"
     CDS             24351..25844
                     /locus_tag="VW411_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU9793415.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            25857..26978
                     /locus_tag="VW411_000634"
     CDS             25857..26978
                     /locus_tag="VW411_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9793416.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLRDHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKRLITKRK
                     FQLDELNLLVKSRFNEMFEEYPDSVFLDTYIKELRAGKSLAGEENNKNKVLKTGAVSY
                     DYFNSSEVKNLPIDYIPLDEHKVEIGDVIISRMNTSELVGAAGYVWAINSDNIYLPDR
                     LWKVILNDRVNPVFLWKLITNEKTKLKIKRISSGTSGSMKNISKSQLLQIRVPFPPLA
                     LQNEFADFVALVDKSQLAIQKSLEELETLKKSLMQEYFG"
     gene            27038..27274
                     /locus_tag="VW411_000635"
     CDS             27038..27274
                     /locus_tag="VW411_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEU9793417.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            27271..27684
                     /locus_tag="VW411_000636"
     CDS             27271..27684
                     /locus_tag="VW411_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEU9793418.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSPSALNMIVNLPEQ
                     FVFGKPLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWISEHSVREKVKK"
     gene            27802..28767
                     /locus_tag="VW411_000637"
     CDS             27802..28767
                     /locus_tag="VW411_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9793419.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKVQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            28801..29907
                     /locus_tag="VW411_000638"
     CDS             28801..29907
                     /locus_tag="VW411_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9793420.1"
                     /translation="MKKVKLGQVATFINGYAFKPQDWSSEGKEIIRIQNLTKTSKGIN
                     YYSGTIDKKYIVEAGDILISWSGTLGVFQWCGRSAVLNQHIFKVVFDKIDIDKSYFKY
                     VVEKGLQDAVKHTHGSTMKHLTKKYFDNIMVSYTNLREQQRIASELDLLSKLILRRQE
                     QLEELNLLVKSRFNEMFGENKIFESIDNLFDIIDGDRGKNYPKSDELFSEEYCLFLNT
                     KNVTKNGFSFDTKQFITKTKDKLLRKGKLERYDIVLTTRGTVGNVAYYDELIKYKHLR
                     INSGMVILRPKTPNLNQKFIIHVLRNNNYSRVISGSAQPQLPITKLKKILLPLPPLAL
                     QNEFADFVVQVDKSQFACEIAIKVWRNSLKFSII"
     gene            29937..33287
                     /locus_tag="VW411_000639"
     CDS             29937..33287
                     /locus_tag="VW411_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU9793421.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIDQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSDHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDNSCEKDIDGSVFNSFIFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSPLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
     gene            complement(33793..34263)
                     /locus_tag="VW411_000640"
     CDS             complement(33793..34263)
                     /locus_tag="VW411_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9793422.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(34280..36553)
                     /locus_tag="VW411_000641"
     CDS             complement(34280..36553)
                     /locus_tag="VW411_000641"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEU9793423.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
     gene            36900..40001
                     /locus_tag="VW411_000642"
     CDS             36900..40001
                     /locus_tag="VW411_000642"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU9793424.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQGVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGQSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRLAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
     gene            40083..41090
                     /gene="pfkA"
                     /locus_tag="VW411_000643"
     CDS             40083..41090
                     /gene="pfkA"
                     /locus_tag="VW411_000643"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEU9793425.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            41149..42654
                     /gene="pyk"
                     /locus_tag="VW411_000644"
     CDS             41149..42654
                     /gene="pyk"
                     /locus_tag="VW411_000644"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU9793426.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(42925..43218)
                     /locus_tag="VW411_000645"
                     /pseudo
     CDS             complement(42925..43218)
                     /locus_tag="VW411_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167986.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(43385..43610)
                     /locus_tag="VW411_000646"
                     /pseudo
     CDS             complement(43385..43610)
                     /locus_tag="VW411_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            44458..45330
                     /locus_tag="VW411_000647"
     CDS             44458..45330
                     /locus_tag="VW411_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEU9793427.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTESTTDVKVHAEKMEAFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
BASE COUNT        13948 a         8035 c         9845 g        13724 t
ORIGIN      
        1 ttattgatga tgtctaaaat ctggatatta gggtctttaa tgtctagtaa ttttgtgata
       61 aaatgtaatt gttccatatg aatctttcta atgagttgtt tggtcgcttt tcattatagg
      121 tcatatggga ctttttttct acaataaaat aggctccata atatctataa gggatttacc
      181 cactacaaat attatagagc caaaaatcct ttgtttacta aacaagggat ttttcttttg
      241 tctctgctcc ttttttgata taatagttct atgttaaaat cagaaaaaca atcacgttat
      301 caaatgttaa atgaagaatt gtccttccta ttggaaggcg aaaccaatgt tttggctaat
      361 ctttccaacg ccagtgctct cataaaatca cgttttccta ataccgtatt tgcaggcttt
      421 tatttgttcg atggaaagga attggtttta ggccccttcc aaggaggtgt ttcctgcatc
      481 cgtattgcac taggcaaggg tgtttgtggt gaggcagctc actttcagga aactgttatt
      541 gttggagatg tgacgaccta tctcaactat atttcttgtg atagtctagc taaaagtgaa
      601 attgtggtgc cgatgatgaa gaatggtcag ttacttggag ttctggatct ggattcttca
      661 gagattgagg attacgatgc tatggatcga gattatttgg aacaatttgt cgctattttg
      721 cttgaaaaga cagcatggga ctttacgatg tttgaggaaa aatcttaatg tatcaagcac
      781 tttatcgaaa atatagaagt caaaacttct cccagttagt tggtcaagaa gttgtggcta
      841 agactcttaa acaagcggtg gagcaagaga aaataagtca cgcttatctt ttttctggtc
      901 ctcgtggaac gggaaaaacc agtgttgcta aaatctttgc caaggctatg aactgtccca
      961 atcaagtggg tggcgaacct tgcaataact gctatatttg tcaagcagtg acggacggta
     1021 gtttagaaga tgtcattgaa atggatgcag cttctaataa tggggtagat gaaattcgcg
     1081 aaattcgtga taaatctacc tatgcgccta gccttgctcg ttataaggtt tatatcatag
     1141 atgaggttca catgctgtct acaggggctt ttaatgccct cctaaagacg ctggaagaac
     1201 caacacagaa tgtagtcttt attttggcca ctactgaatt gcacaagatt cctgctacta
     1261 ttctatcccg tgtgcaacgt tttgagttta aatcaattaa gacacaggat attaaggaac
     1321 atattcacta tatcttagaa aaagaaaata tcagttctga accagaggct gtggaaatca
     1381 ttgccagacg ggcggaaggt ggaatgcggg acgccttgtc tattttggat caagccctga
     1441 gtttgacaca gggaaatgag ctgacgactg ctatctctga agaaattact ggcaccatta
     1501 gcctatcagc cttggatgat tatgtggcgg ccttgtctca acaggatgtt cccaaagctt
     1561 tgtcttgctt gaatcttctt tttgacaatg gtaagagcat gactcgtttt gtgaccgatc
     1621 ttttgcacta tttaagagac ttgttaattg ttcaaacagg gggagcaaat actcatcata
     1681 gttcagtctt tgtagaaaat ttggcacttc ctcaaaaaaa tctgtttgaa atgattcgct
     1741 tagcaacagt gagtttagca gatattaagt ctagtttgca acccaagatt tatgctgaaa
     1801 tgatgaccgt ccgtttggcg gaaatcaagt ccgaaccagc tctatcagga gcggttgaaa
     1861 atgaaattgc tacgctgaga caggaagttg cccgtctcaa acaagagctt tctaatgtag
     1921 gtgcggttcc taaacaagtt gcaccagctc ctagtcgacc agctacgggc aaaacagtct
     1981 atcgtgtcga tcgcaataaa gtgcaatcta tcttacaaga ggccgtcgaa aatcctgatt
     2041 tagcacgtca aaatttaatt cgtttgcaga atgcctgggg agaggtaatt gaaagtctag
     2101 gtgggccgga caaggctctg ctagttggtt ctcaaccggt tgctgccaat gaacaccatg
     2161 ctattcttgc ttttgagtct aacttcaatg ctggtcaaac tatgaaacga gacaatctca
     2221 ataccatgtt tggtaatatc ctcagtcagg cggcaggttt ttcacctgag attttagcta
     2281 tttccatgga ggaatggaaa gaagttcgcg cagccttttc agccaaagcc aaatcttctc
     2341 aaactgaaaa agaagtagaa gaaagcctga ttccagaagg atttgaattt ttggctgata
     2401 aagtgaaggt agaggaagac taaagaaaga tttcatgata caataagttt atgaataaac
     2461 aacaatttat tattatggcg ctgtttacag ctgctgagac ctattttttc aatgaagcct
     2521 ggatgactgg ccgctatatt atggcagcct tttgggcaat tttactcttt agaaatttcc
     2581 gagtcagtta tgtgatgggc aaaatcgttg atgtcatcga tcagcatttt aataggaaag
     2641 actagccctc agcttccaga caaaatcaaa gccttttagg cttttttttg ttatactaga
     2701 aaagtatatt tatagaattt ttgctctatt tctggggaaa tcagacgttt ttctagtaag
     2761 tactgtaaaa gttttgaaaa agaaaggaac tatcatgtca gtattagaga tcaaagatct
     2821 tcacgttgag attgaaggaa aagaaatttt aaaaggggtt aacctgaccc tgaaaacagg
     2881 agaaattgcc gctatcatgg gaccaaatgg tacaggtaaa tcgactcttt ctgccgctat
     2941 catgggaaat ccaaactatg aagtaactaa aggtgaagtt ttgtttgatg gcgtaaacat
     3001 ccttgagttg gaagtggatg agcgtgcgcg tatgggactt ttccttgcta tgcaataccc
     3061 atcagaaatc cctggaatta ccaatgctga gtttcttcgt gccgctatga atgcgggtaa
     3121 agaagatgat gagaagattt cagttcgtga gtttattact aagctagatg aaaaaatgga
     3181 attgctcaac atgaaagaag aaatggcaga gcgttacctc aacgaaggct tctctggtgg
     3241 tgagaaaaaa cgcaatgaaa ttcttcaact tttgatgttg gagccaacat ttgctctttt
     3301 ggacgagatt gactcaggtc ttgatattga cgctcttaaa gttgtgtcta aaggtgtcaa
     3361 tgccatgcgt ggtgaaggtt ttggtgctat gatcatcact cactaccaac gtcttttgaa
     3421 ctatatcaca cctgatgtgg tacacgtgat gatggaaggt cgtgttgtcc tttctggtgg
     3481 tccagaattg gctgcgcgtt tggaacgtga aggatacgca aaattagctg aagaacttgg
     3541 ctacgactac aaggaagaat tgtaattccc tcgtatcttt taggagaagt aaatgactag
     3601 agaaaatatt aaactttttt cagaaatgca cgctgaacca agctggttgg ctgatctccg
     3661 tcaaaaagct tttgacaaga ttgagacttt ggaattacca gttattgagt gtgtcaaatt
     3721 ccaccgttgg aatctgggtg atggaacgat tacagaaaat gagccatcag caaatgttcc
     3781 agatttcaca gctttagatc atcacttgaa gttggtgcaa gtaggaactc aaactgtttt
     3841 cgaacaaact ccagttgagt tagctgaaca gggtgttgtc ttcacagact ttcactcagc
     3901 tttagaagaa attccagagc tgatcgaaga attcttcatg tcatctgtta agtatgatga
     3961 tgacaagttg gcggcttacc acacagctta ctttaacagt ggtgctgtac tctatattcc
     4021 agataacgta gaaatcacag agccaattga aggaattttc taccaagata gcgatagcaa
     4081 tgtgccgttt aacaagcata ttatgattat cgttggtaaa aattctaaga ttagttatct
     4141 ggagcgttta gagtcacgcg gtgaaggaag tgacaaagca actgccaata tcacagtgga
     4201 agtgattgca cgttctggtg cgcaagtcaa gtttgctgct atcgaccgtc taggtgaaaa
     4261 cgtcactgcc tacattagcc gtcgtggtaa attaggcaac gatgcaagta ttgactgggc
     4321 tatcggtgtc atgaacgaag gaaatgtcgt tgctgatttt gatagtgact tgattggtaa
     4381 tggtagccat gctgacctca aggttgtagc tctttcaagt ggtcgtcagg tacaagggat
     4441 tgatactcgt gtaactaact atggctgcaa ctcaatcgga aacattctac aacatggggt
     4501 tatccttgaa aaagcaactt tgactttcaa tggtatcggc cacatcatca agggtgctaa
     4561 gggagcagat gcgcaacaag agagccgtgt tctcatgctt tcagaccaag cgcgttcaga
     4621 tgctaaccca attcttttga ttgatgaaaa tgacgtaact gcaggccatg cagcctctat
     4681 tggtcaggta gatccagaag atatgtacta cctcatgagt cgtggcttgg ataaggcaac
     4741 tgcagagcgt ttggttgttc gtggtttcct tggatctgtt atcgtggaga ttccagtcaa
     4801 ggaagttcgt gatgaaatga ttgcaactat cgaagagaaa ttgtcaaaac gctaaggggc
     4861 agcctatgtt agatgtagaa gcgattcgca aggattttcc aattttagat cagattgtca
     4921 atgatgaacc tctggtctat ctggacaatg ctgcgacgac acaaaaacca ctagtagttc
     4981 tgaaagctat taacagctac tatgagcagg acaatgccaa tgttcaccgt ggtgtccata
     5041 ccttagcgga acgagcgaca gcttcttatg aagctgctcg tgaaaccatt cgtaagttta
     5101 ttaatgcagg ctctacaaag gaagttctct ttaccagagg aacgacaacc agccttaact
     5161 gggtggcacg ctttgctgag gaaattctca ctgagggaga ccaggtcttg atttcagtaa
     5221 tggaacacca ttctaatatc attccatggc aggaagcttg tcgaaagact ggagcagagc
     5281 ttgtctatgt ctatcttaaa gacggtgcct tggatatgga ggatttgcga gctaaattga
     5341 ctgataaggt taaatttgtt tccctagctc atgcctccaa tgttcttggt gtggtcaatc
     5401 cgatcaagga aatcactcaa ttagcccacc aagttggggc aattatggta gtggatggtg
     5461 ctcaatctac acctcatatg aagattgatg tccaggactt ggatctggac tttttcgcct
     5521 tttcgggtca caagatggct ggtccgactg gtatcggtgt cctttacggc aaagaaaagt
     5581 atcttgagca aatgtctcca gtagaatttg gcggcgagat gattgatttt gtctacgagc
     5641 aatttgctag ttggaaggaa ttgccttgga aatttgaggc tggaacgcca aatatggcag
     5701 gagctattgg acttgcgact gcagttgatt atctggaaaa gattggtatg gatgccgttg
     5761 aagctcatga acaggaattg attgcgtacg tctatccaaa actgcaggca attgagggat
     5821 tgaccattta cggttctcag gatttggctc aacgttcggg tgttattgcc tttaacctag
     5881 gtgatctcca tcctcacgat cttgcgacgg ctctggatta tgaaggagtg gctgttcgtg
     5941 ctggtcacca ttgtgcgcaa cccttgcttc agtatttgga agtcccagca acagctcgtg
     6001 caagttttta tatctacaat accaaggcag attgcgacaa actagtcgat gccctacaaa
     6061 agacaaagga gtttttcaat ggcactttct aaactagata gcctttatat ggcagtggta
     6121 gcagaccatt cgaaaaatcc acatcaccaa gggaagttag aagatgctga gcaaatcagt
     6181 ctcaacaatc cgacttgtgg ggatgtcatc aacctctctg tcaagtttga tgcagaggac
     6241 cgtttggaag atattgcttt tctaaattca ggatgcacga tttcaactgc ttctgctagt
     6301 atgatgacag atgccgtttt aggaaaaacc aaacaagaaa ttttagaact ggcgactatt
     6361 ttttctgaaa tggttcaagg gcaaaaagat gagcgtcaag accaacttgg agacgcggca
     6421 ttcttgtcag gtgttgccaa attccctcaa agaatcaagt gtgcaaccct agcttggaat
     6481 gcccttaaga aaacaattga aaatcaagaa aaacagtaag acaagtttct tttgtcttat
     6541 gaattattag aaatgaagaa agaaaggata ctatggctga agaaagagta gaaccaaaac
     6601 caattgacct tggtgaatat aaatttggtt tccatgacga tgtagagcct gtcttatcga
     6661 caggaaaagg actcaacgaa ggtgttattc gtgaattatc tgctgctaag ggtgagcctg
     6721 agtggatgtt ggagttccgt ttgaagtctt atgaaacctt caaaaaaatg cccatgcaaa
     6781 cttggggagc agacttgtca gagattgact ttgatgactt aatctactac caaaaaccat
     6841 ctgacaaacc agcccgttct tgggatgatg tacctgaaaa gattaaagaa acctttgaac
     6901 gtatcgggat tccagaagct gaacgtgctt atttagcagg ggcttctgcc cagtacgagt
     6961 cagaagtggt ttaccacaac atgaaggaag agttccaaaa attaggtatt atctttacag
     7021 atacagattc cgcactcaag gaatacccag acttatttaa acaatacttt gcgaagttgg
     7081 taccgccgac agataacaag ttggcagccc tcaactcagc agtatggtcg ggtggaactt
     7141 ttatctacgt gccaaaaggt gtcaaggtag atattccact tcaaacttat ttccgtatca
     7201 ataacgaaaa tataggtcag ttcgaacgta ccttgattat cgttgatgag ggagcaagcg
     7261 tctactacgt agaaggatgt acagcaccaa catattcaag caatagctta cacgctgcca
     7321 ttgtagaaat ttttgctttg gacggagctt atatgcgtta tacaactatc caaaactggt
     7381 ctgataacgt ctataacttg gtaacaaagc gtgctaaggc tcaaaaggat gccactgttg
     7441 agtggattga tggaaacttg ggtgccaaaa cgactatgaa atatccatct gtttaccttg
     7501 atggagaagg agcgcgtggt accatgctct ctatcgcctt tgctaatgca gggcaacacc
     7561 aagacacggg tgctaagatg attcacaatg ctccacatac cagctcgtct attgtgtcta
     7621 aatccatcgc taaaggtgga ggaaaggttg actaccgtgg acaagtcacc tttaacaaga
     7681 actctaagaa atctgtttcc cacattgaat gtgataccat tatcatggat gacttgtcag
     7741 catcagatac tattccattt aatgaaattc acaactcgca agtggctttg gaacacgaag
     7801 ccaaagtatc taagatttca gaagagcaat tgtattatct catgagccgt ggattgtcag
     7861 aatctgaggc aactgaaatg attgtcatgg gatttgtaga accctttaca aaagaacttc
     7921 caatggaata cgcagttgag ctgaaccgct tgattagcta tgaaatggag ggatcagttg
     7981 gataaaattt gattttatac tcttcgaaaa tctcttcaaa ccacgtcagc atcgccttac
     8041 cgtatgtatg gttactgact tcgtcagttt catctacaac ctcaaaacag tgttttgagc
     8101 aacctgcggc tagcttccta gtttgttctt tgattttgag tattagattt actcaaaatc
     8161 aaggattttg aagatgaact tgtatcaaaa aatcgcggtt taaaatcgcg attttttata
     8221 atttctcgtt aacaaagcgg acaaactgat tccaccaaac ttttaagaag aaggcttttt
     8281 caattttctt gtctgctacc atttcgaaac tagggcgctc tgtggtgatg taaccttgac
     8341 caatcaagtc cttgtcttca taagtcaaat ggccaaccac tgttccagct tcaagtggtg
     8401 ctgggattgc tttggaatca ggtgtgaatt gaacagattg ggaagattga ttcccaacac
     8461 gttcgattag atagatatcc tctggagcca ctgcagttac tgtatcttct tttccatctt
     8521 gtacaggggc tttgctatct tgataggcat cgccttgttg aacgattttg cgaagtgtaa
     8581 atgtagaaga aatataatcc attagggaag atgtagctgt aaatcgagcg taaggattat
     8641 tgtcttgatg atctgcattt aaaacaactg tgatgactct catgcctttt tcgacagtag
     8701 taccaacaaa agactctcca gccttatctg ttgttcctgt ttttagccca tcaaaaccac
     8761 cacggtaagc aggcatacct tctaacatgt agttggttga agtgattgtc atcccagcaa
     8821 aagtagaaga aggttttttg gtgatttcta agacttgtgg gtattttttg atgaggttgc
     8881 gagcaacgat agcgacatca taagcactaa gcttattttc ctcatctttt ttagaacctg
     8941 ggtaaatgtt atcccctaga gtttcattgt taagacctgt cgtattgaca acagtggcat
     9001 cctgaattcc ccattccaag agttttgccc gcatcatatc gacgaaatct ttttctgagc
     9061 cagcaatttt ctcagctagg gcaatagcgg cgctgttggc actagatacc agagttgctt
     9121 caagcaactc ttcgacagta taattacggg cctccatagg aatattactg gcttcagaat
     9181 ttgtcgtcaa ttgataagga taatcagaaa tatctacagg agtggagagg gtaatacttc
     9241 cgttttccaa agcttcatag accagataaa cagtaatcaa ttttgttatg gaagcaattt
     9301 cgacaggttg cgttgcatcc ttctcataga gaattttacc agtatttgcc tcaacagcaa
     9361 tcgcatgttt agcggcaatg gtaaaatctt gagcaacagc agtagaagca ccccctaaaa
     9421 gagagacagt taacaaagtt aaaaatattt ttttcatagt agtcttattc tatcataaag
     9481 aaaaaaaata ttcttgcttt aataattcat ctgttaagct ttttgaaaat atggtaaaat
     9541 aaagtaaggg aggtaactca tgtttcgtag aaataaatta tttttttgga ccacagaaat
     9601 tttactctta accatcatct tttacctatg gagacagatg gggtctttga ttaacccttt
     9661 tgttagcgtg cttaatacaa ttatgattcc atttttatta gggggctttt tttattattt
     9721 gacaaaccct attgttactt tcttaaataa agtctgtaaa ctcaatcgtt tgcttggtat
     9781 tttaattacc ttgtgtactt tggtctgggg aatggtcata ggtgttgtct atctcttacc
     9841 tattttgatt aatcagttat ctagtttgat tatatctagt caaactattt atagtcgagt
     9901 acaagactta atcatagact tatctaatta tcctgcgctc cagaatttgg atgtagaagc
     9961 tacaattcag cagttaaact tatcctatgt tgatattctt caaaatatcc taaatagcgt
    10021 atcaaatagt gtggggagcg tcttgtcagc tcttatcagt actgttttga ttttgattat
    10081 gactccagtt tttttggttt atttcttatt agatggacat aaattcttgc ccatgcttga
    10141 aagaacgatt ctaaagaggg atcgcttgca tattgcaggc ttattaaaga atttaaatgc
    10201 gacgattgct cgctatatta gtggagtttc gattgacgca atcattatag gttgtttggc
    10261 ttatattggc tatagtatta ttggtttaaa atatgcttta gtttttgcca ttttttctgg
    10321 tgtagccaat ttaattcctt atgtggggcc aagtattggt ttgattccta tgatcatcgc
    10381 aaatatattc actgatcccc atagactgct gattgcagtg atttatatgc ttgttgttca
    10441 gcaggtagat ggcaatatct tatatcctcg aatcgtagga agtgttatga aggttcatcc
    10501 aatcacgatt ttagttttac ttttgttgtc aagcaatatc tatggtgtag ttggaatgat
    10561 tgtcgcagtg ccaacctatt ctatcttgaa agaaatttct aagttcttat cccatttgta
    10621 tgaaaatcat aaaataatga aagaacgaga aagagaatta gctaagtaaa agtcaggaga
    10681 accctgattt ttctttactg gaagtggcct ttagattaga agactgaaaa taagttaaag
    10741 tcttaaacta attttcacag ctaagaatag tagaagttaa tctgataaaa atcgaaaaaa
    10801 ccagtggaat tctgtgtcag ggtaagttcc actggttttc atagtctatt aaagttcgaa
    10861 tgaaacctat ttatagtaga ttgaaactag aatagtacac ctctaattct aaaacattgt
    10921 tagaaatcga tttgactgtc ctgatctatt cgttctattc ttatttcatt ttactatatt
    10981 ttggtgcaat aagtgaaaag tagtccgaat aatataagga ttgattttat agtttttaaa
    11041 ctcaaatgaa ttgaaataaa gagagtacga aaattctcat ctgaaagtat tttagaataa
    11101 ttctcttcgt gaatttcttc aaaacagata gcttcatctt aggtatgtga tttctttttg
    11161 catttttgag ttagataagg tataatgatt ttattgtctt ttggggtcgt tacggattcg
    11221 acaggcatta tgaggcatat tttgcgactc gtgtggcgac gtaaacgctc agttaaatat
    11281 aactgcaaaa aataacactt cttacgctct agctgcctaa aaaccagcag gcgtgacccg
    11341 atttggattg ctcgtgttca atgacaggtc ttattattag cgagatacga ttaagccttg
    11401 tctagcggtt tgataagaga ttgatagact cgcagtttct agacttgagt tatgtgtcga
    11461 ggggctgtta aaataataca taacctatgg ttgtagacaa atatgttggc aggtgtttgg
    11521 acgtgggttc gactcccacc ggctccatta ttcctttgca ttcttttgca ttccttggta
    11581 aaacgttgtt aaatcaacgt tttttatttt tatctttggt attcctttgc attcttttgc
    11641 taaaaaggga gtcacaaaca gaccctattt taaaaaaagg atagaaaaaa ggatacaaca
    11701 tttgtcgcat cctaaaaata atcttttttc gacggaagac atgggattcg aacccacgca
    11761 cgctattaca cgcctaccgc gtttccaaca cggcctctta agcctcttga gtaatcttcc
    11821 aatacttact caaatagtct accataaagg ctcttatctt gcaataaaaa ttctagaaat
    11881 aagaaaaatg atagattttg aaagaaaatg ataaaaaatg cttgacttcg aaagaaagta
    11941 tgatagaatg aatagtgtaa acgataacag gaggtgattc agtgttaaaa acagaacgta
    12001 aacaactaat tttagaggag ttaaatcaac atcatgtagt ttctctagaa aaattagtta
    12061 gtttgctaga aacgtcagaa tcaacggttc gaagagactt ggatgagttg gaagcggaaa
    12121 acaagcttcg tcgtgtgcat ggtggagcag aactccccta ctccttacag gaagaagaaa
    12181 ccattcaaga aaaatctgtc aaaaaccttc aagaaaagaa attgctggct cagaaagcag
    12241 cctctctcat taaagaaaaa gatgtcatct ttatcgatgc tggaacaaca actgcttttt
    12301 tgattcatga attggtcaat aagaatgtta cagttgtgac caactccatt caccatgccg
    12361 ctcagttggt tgaaaagcag attccaactg tcatggttgg aggaaacgtc aagacggcga
    12421 cagatgctag tatcgggggc gttgctctta accagattaa ccaattgcac tttgaccgtg
    12481 cctttatcgg aataaatggt gttgacgatg gctattatac gactcctgat atggaggagg
    12541 gagctgtgaa aagagctatt ttggagaatg ccaagcagac ctacgtcttg gtggattcgt
    12601 caaaaattgg acaaacttgc tttgccaagg tagccccact caaacgcgct atcgttatca
    12661 ctagtcaagg gcatgagctc ttgcaggtta ttaaggagaa aacggaggta atagaagtat
    12721 gatttataca gtcacactca atccatccat tgactatatc gttcgtttgg accaagtcaa
    12781 agttggtagt gtcaatcgta tggacagtga tgataagttt gctggtggga aaggaatcaa
    12841 tgtcagccgt gtcttgaaac gtttgaatat accaaataca gcgacgggat ttatcggtgg
    12901 ctttactggt aaatttatca cagatacttt agcagaggaa gaaatcgaga cacgttttgt
    12961 ccaggtggca gaagatactc gtatcaatgt taaaatcaaa gcagaccaag aaacagaaat
    13021 caacggaacg ggtccaactg ttgaatcggt tcagctagaa gaattgaaag ctattttatc
    13081 tagtctgaca gcagaagata cagttgtctt tgcaggttca agtgctaaaa atctaggcaa
    13141 tgttatctat aaggatttga tttccttgac gcgccagact ggtgcgcaag tggtctgtga
    13201 ctttgaagga cagaccttaa ttgatagttt ggactaccag cctcttcttg taaaaccaaa
    13261 caatcatgaa cttggagcga tttttggggt taaactcgaa agtttagatg aaattgagaa
    13321 atacgctcgt gagttactgg ctaagggtgc tcaaaatgtt attatctcta tggctggtga
    13381 tggtgccctt cttgtcacat ctgagggagc ttacttcgct aaaccaatca aaggaacagt
    13441 caaaaattca gttggagctg gtgattctat ggttgctgga ttcacaggtg aatttgtcaa
    13501 atcaaaagac gtagtagaag ccttcaaatg gggagtggct tgcggaacgg caactacctt
    13561 ctcagatgac ttggcaacgg cggaatttat taaagaaaca tatggaaaag ttgaggtaga
    13621 aaaacgatga aaattcaaga cctattgaga aaagatgtca tgttgctaga tttgcaggca
    13681 actgaaaaaa cagctgtcat cgacgagatg attaaaaatt tgacagacca cggttatgta
    13741 acagattttg aaacatttaa agaaggaatt ttggcgcgtg aagctttgac ttctactggt
    13801 ttgggtgatg gaatcgcaat gcctcacagc aaaaacgctg ctgtcaaaga agcgacagtt
    13861 ctatttgcta agtcaaataa gggtgttgac tacgagagct tggatggaca agcaactgac
    13921 ctcttcttca tgattgcagc tccagaaggt gccaatgata ctcacttggc agccttggca
    13981 gaattgtctc aatacttgat gaaagacggt tttgcagaca aacttcgtca agcaacatct
    14041 gcagaccaag ttatcgaact ttttgaccaa gcttcagaaa aaactgagga acttgttcaa
    14101 gcacctgcta atgactctgg tgactttatc gtagctgtta cagcttgtac aacaggtatt
    14161 gcccacactt acatggccca agaagccctt caaaaagtag ctgctgaaat gggggttggt
    14221 atcaaggtcg aaaccaacgg tgctagcggt gttggaaatc aactaactgc agaagatatc
    14281 cgtaaggcta aagctattat cattgcagca gacaaggccg ttgaaatgga tcgatttgat
    14341 ggaaaaccat tgatcaatcg tccagttgct gacggtatcc gtaagacaga agagctaatt
    14401 aacttggctc tttcaggaga tactgaagtc taccgtgccg ctaatggtgc caaagctgca
    14461 acagcctcta acgaaaaaca aagccttggt ggtgccttgt acaaacactt gatgagtggt
    14521 gtatctcaaa tgttaccatt cgttatcggt ggtggtatca tgattgccct tgccttcttg
    14581 attgacggtg ctttgggtgt tccaaatgaa aaccttggca atcttggttc ttaccatgag
    14641 ttagcttcta tgttcatgaa aattggtgga gctgcctttg gtttgatgct tccagtcttt
    14701 gcgggttatg ttgcctactc tattgctgaa aaaccgggtt tggtagcagg tttcgtggct
    14761 ggtgctattg ccaaagaagg ttttgccttt ggtaaaattc cttatgccgc aggtggtgaa
    14821 gcaacttcaa ctcttgcagg tgtctcatct ggtttcctag gtgcccttgt tggtggattt
    14881 atcgcaggtg ccttggttct tgccatcaag aaatacgtta aagttcctcg ttcactcgaa
    14941 ggtgctaaat caatccttct attgccactt cttggaacaa tcttgacagg atttgttatg
    15001 ctagctgtga atatcccaat ggctgcaatc aacactgcta tgaatgactt cctaggcggt
    15061 cttggaggag gttcagctgt ccttcttggt atcgtccttg gtggaatgat ggctgttgac
    15121 atgggtggac cagttaataa agcagcttat gtctttggta caggtacgct tgcagcaact
    15181 gtttcttcag gtggttctgt agccatggca gcagttatgg ctggaggaat ggtgccacca
    15241 cttgcaatct ttgtcgcaac tcttcttttc aaagataaat ttactaagga agaacgtaac
    15301 tctggtttga caaacatcat catgggcttg tcatttatca ctgagggagc gattccattt
    15361 ggtgccgctg acccagctcg tgcgattcca agcttcatcc ttggttcagc agtagcaggt
    15421 ggactcgttg gtcttactgg tatcaaactc atggcgccac acggaggaat cttcgttatc
    15481 gcccttactt caaatgctct cctttacctc gtttctgtct tggtaggagc aatcgtaagt
    15541 ggtgtggttt atggttacct acgcaaacca caagcataaa aaatagaaaa atgaaaagat
    15601 tggaccgttt ggtgcagtct ttttctcttc ccgaaatgcc tgtgaaatat ggtataatag
    15661 aagaatggca aacaagaata caagtacaac aagacggaga ccgtctaaag cagaactgga
    15721 aagaaaagaa gcgattcaac gaatgttgat ttcgttagga attgcgattt tattgatttt
    15781 cgcagccttc aaattagggg ctgcaggtat aaccctttat aatttaattc gcttgctagt
    15841 gggtagccta gcttatctgg cgatattcgg cctattaatc tatctcttct ttttcaagtg
    15901 gatacgaaaa caggaaggac tcttatctgg ctttttcacc atatttgctg gcttactctt
    15961 gatttttgag gcctacttgg tttggaaata tggtttggac aagtccgttc taaaagggac
    16021 catggctcag gttgtgacag atctgactgg ttttcgaacg actagctttg ctggaggggg
    16081 cttgatcggg gtcgctcttt atattccaac agcctttctc tttttaaata tcggaactta
    16141 ctttattggt tctatcttga ttttagtggg ttctctccta gtcagccctt ggtctgttta
    16201 cgatattgct gaatttttca gtagaggctt tgccaaatgg tgggaagggc acgagcgtcg
    16261 aaaagaggaa cgctttgtca aacaagaaga aaaagctcgc caaaaggctg agaaagaggc
    16321 tagattagaa caagaagaga ctgaaaaagc cttactcgat ttgcctcctg ttgatatgga
    16381 aacgggtgaa attctgacag aggaagctgt tcaaaatctt ccacctattc cagaagaaaa
    16441 gtgggtggaa ccagaaatca tcctgcctca agctgaactt aaattccctg aacaggaaga
    16501 tgactcagat gacgaagatg ttcaggtcga tttttcagcc aaagaagccc ttgaatacaa
    16561 acttccaagc ttacaactct ttgcaccaga taaaccaaaa gatcagtcta aagagaagaa
    16621 aattgtcaga gaaaatatca aaatcttaga agcaaccttt gctagctttg gtattaaggt
    16681 aacagttgaa cgggccgaaa ttgggccatc agtgaccaag tatgaagtca agccggctgt
    16741 tggtgtaagg gtcaaccgca tttccaatct atcagatgac ctcgctctag ccttggctgc
    16801 caaagatgtc cggattgaag caccaatccc tgggaaatcc ctaatcggaa ttgaagtgcc
    16861 caactccgat attgccactg tatctttccg agaactatgg gaacaatcgc aaacgaaagc
    16921 agaaaatttc ttggaaattc ctttagggaa ggctgttaat ggaaccgcaa gagcttttga
    16981 cctttctaaa atgccccact tgctagttgc aggttcaacg ggttcaggga agtcagtagc
    17041 agttaacggc attattgcta gcattctcat gaaggcgaga ccagatcaag ttaaatttat
    17101 gatggtcgat cccaagatgg ttgagttatc tgtttacaat gatattcccc acctcttgat
    17161 tccagtcgtg accaatccac gcaaagccag caaggctctg caaaaggttg tggatgaaat
    17221 ggaaaaccgt tatgaactct ttgccaaggt gggagttcgg aatattgcag gttttaatgc
    17281 caaggtagaa gagttcaatt cccagtctga gtacaagcaa attccgctac cattcattgt
    17341 cgtgattgtg gatgagttgg ctgacctcat gatggtggcc agcaaggaag tggaagatgc
    17401 tatcatccgt cttgggcaga aggcgcgtgc tgcaggtatc cacatgattc ttgcaactca
    17461 gcgtccatct gttgatgtca tctctggttt gattaaggcc aatgttccat ctcgtgtagc
    17521 atttgcggtt tcatcaggaa cagactcccg tacgattttg gatgaaaatg gagcagaaaa
    17581 acttcttggt cgaggagaca tgctctttaa accgattgat gaaaatcatc cagttcgtct
    17641 ccaaggctcc tttatctcgg atgacgatgt tgagcgcatt gtgaacttca tcaagactca
    17701 ggcagatgca gactacgatg agagttttga tccaggtgag gtttctgaaa atgaaggaga
    17761 attttcggat ggagatgctg gtggtgatcc gctttttgaa gaagctaagt ctttggttat
    17821 cgaaacacag aaagccagtg cgtctatgat tcagcgtcgt ttatcagttg gatttaaccg
    17881 tgcgacccgt ctcatggaag aactggagat agcaggtgtc atcggtccag ctgaaggtac
    17941 caaacctcga aaagtgttac aacaataaaa aaatagcttc tttccaagtt tggagggaag
    18001 ctattttagt ggctattgat tgcttttatt ttctgaagtt ggcgcattgg actgtttttc
    18061 gttttcagta gcaggtttac ttgaagcagg agtagaagag tcctgagttg ctgttttctg
    18121 atcttctttt ttctcttcct tgacgctaga ttttggtgtt tcctcttgct gtgttttttc
    18181 ttgactagtg ttagtctctt tagttggact ggtgttttcc ttaggggatt ccttttggat
    18241 ttctttgaca atggttgtcg tctggcttgt cgtaggttct tttttaatat ttttgttatt
    18301 atccaaggcg ttcataatcg tgatacttgc ggtaatcaag ccaaggatac tagcgattgt
    18361 agcaacggtt ttttgaaaga ctgtaaagcc ttttttgatg tgttctgttt ttggttttga
    18421 agttctacga tagttagaca tacattctct cctttgatta tgatttatta tactctgttt
    18481 ctttaaaaaa atcttaaaaa aagagaaatt tccctttctt gtcgcaagct ccgtttcatg
    18541 atacaataga ggaagtgatt ttagaaagcg tgagaaacat gatttacttt gataattcag
    18601 cgacgaccaa gccttatcct gaagcccttg aaacctatat gcaggtcgct tcaaaaattt
    18661 taggaaaccc atctagtctc catcgtttgg gagaccaagc aacacgaatc ttagatgctt
    18721 ctcgccaaca gattgcagat ttaatcggta agaaaagcga tgaaatcttc tttacctcgg
    18781 gtggaacaga aggggataac tggcttatca agggtgtggc ctttgaaaaa gctcagtttg
    18841 gcaagcacat cattgtttca gccattgaac atccagcagt caaagagtca gccctctggt
    18901 tgaaaagtca aggatttgaa gtggattttg ctccagttga taagaaaggc ttggtcgatg
    18961 ttgaggcgtt agcaggtttg atacggcatg atacaatcct cgtttccatc atggctgtga
    19021 acaatgaaat cggctctatc caacctattg aggctatttc agaattcttg gcagacaagc
    19081 cgactatttc cttccacgtt gatgcggttc aggcgcttgc caaaatttcg actgaaaagt
    19141 atctgacaga acgggtggat tgcgcgactt tctctagtca caagttccac ggggttcgag
    19201 gtgttggctt tgtctatatc aaatctggca agaagattac acctcttctt acaggtggtg
    19261 gccaggagcg agattatcgt tcgacaactg aaaatgtggc agggattgca gcgacagcca
    19321 aggccctccg tttgtctatg gaaaagctag atatctttag gagcaagact gggcagatga
    19381 aggcagtgat tcgccaagct cttctgaact atccggatat ttttgtcttt tcagatgagg
    19441 aaaactttgc acctcatatt ctgacttttg gaatcaaagg tgttcgaggt gaagtcatcg
    19501 ttcacgcctt tgaagactat gatattttca tctcaacaac ctcagcttgt tcatctaagg
    19561 caggaaaacc agccggtacc ttgattgcca tgggagtgga caaagataag gccaagtcag
    19621 ctgtgcgtct tagcctagac ttggaaaatg atatgagtca ggtcgagcag tttttgacca
    19681 agttaaaatt gatttacaat caaactagaa aagtaagata ggagcattca tgcagtattc
    19741 agaaattatg attcgctacg gagagttgtc aaccaagggt aaaaaccgta tgcgtttcat
    19801 caataaactt cgtaataata tttcggacgt tttgtctatc tatacccaag ttaaggtaac
    19861 agcagatcgc gaccgtgccc acgcttacct caatggagct gattacacag cagttgcaga
    19921 atctctcaaa caagtttttg gaattcaaaa cttttctcct gtttataagg ttgaaaaatc
    19981 tgtagaagtt ttgaagtctt ctgtccaaga gattatgcgg gacatctaca aggaaggtat
    20041 gacctttaag atttctagca agcgtagcga ccacaacttt gaacttgata gtcgtgaact
    20101 caaccaaaca cttggagggg ctgtattcga agccattcca aatgtgcaag ttcaaatgaa
    20161 aagtcctgac atcaatcttc aggtggagat tcgtgaagaa gcagcctatc tttcttatga
    20221 aaccattcgt ggggctggtg gtttgccagt tggaacttca ggtaaaggga tgctcatgtt
    20281 gtcaggaggg attgactcac ctgtagcagg ttatcttgct cttaagcgtg gggtggatat
    20341 cgaggcagtt cactttgcta gtccaccata tactagtcct ggtgccctca agaaagcgca
    20401 ggacttgacc cgtaaattga ccaagtttgg cggaaatatc cagtttatag aggtgccttt
    20461 cacagagatt caagaggaaa tcaaagccaa agcgccagaa gcttatttga tgactctaac
    20521 tcgtcgcttt atgatgcgga ttactgaccg tattcgtgag gtacgaaatg gtttggttat
    20581 catcaatggg gaaagtctag gtcaagtagc cagccaaacc cttgaaagta tgaaggctat
    20641 caatgctgtt accaacactc ccatcattcg tcctgtggtt accatggaca agttggaaat
    20701 cattgacatc gcccaggaaa tcgatacctt tgacatttca atccaaccgt ttgaagactg
    20761 ttgtaccatt tttgcaccag atcgtccaaa aacaaatcct aaaattaaga atgcggagca
    20821 gtacgaagcg cgtatggatg ttgaaggctt ggttgagcga gcagtggctg gaatcatgat
    20881 tactgaaatc acacctcaag ccgaaaaaga tgaagttgat gacttgattg acaatctgct
    20941 ctaattcaga aaatccaaaa gaatagcgaa aatcagtaaa aaaagttagt tttttctcta
    21001 aaaacaggta aaaaactaac tttttttatt tttatgatat aatgatataa aattttgaat
    21061 atagagagtt ttctgacaat gaatcaatcc tacttttatc taaaaatgaa agaacacaaa
    21121 ctcaaggttc cttatacagg taaggagcgc cgtgtacgta ttcttcttcc taaagattat
    21181 gagaaagata cagaccgttc ctatcctgtt gtatactttc atgacgggca aaatgttttt
    21241 aatagcaaag agtctttcat tggacattca tggaagatta tcccagctat caaacgaaat
    21301 ccggatatca gtcgcatgat tgtcgttgct attgacaatg atggtatggg gcggatgaat
    21361 gagtatgcgg cttggaagtt ccaagaatct cctatcccag ggcagcagtt tggtggtaag
    21421 ggtgtggagt atgctgagtt tgtcatggag gtggtcaagc cttttatcga tgagacctat
    21481 cgtacaaaag cagactgcca gcatacggct atgattggtt cctcactagg aggcaatatt
    21541 acccagttta tcggtttgga ataccaagac caaattggtt gcttgggcgt tttttcatct
    21601 gcaaactggc tccaccaaga agcctttaac cgctatttcg agtgccagaa actatcgcct
    21661 gaccagcgca tcttcatcta tgtaggaaca gaagaagcag atgatacaga caagaccttg
    21721 atggatggca atatcaaaca agcctatatc gactcgtcgc tttgctatta ccatgatttg
    21781 atagcagggg gagtacatct ggataatctt gtgctaaaag ttcagtctgg tgccatccat
    21841 agtgaaatcc cttggtcaga aaatctacca gattgtctga gattttttgc agaaaaatgg
    21901 taagttaaga aaggaaaaaa cgaaatgcat attgaacatc ttagccactg gagtggtcat
    21961 cttaaccgtg aaatgtacct taaccgttat ggacatggtg ggattccagt tgtggtcttt
    22021 gcttcatcag gtggtagtca caacgaatac tatgattttg gcatgattga tgcctgtgct
    22081 tcctttatcg aggaaggcct tgtccagttc tttaccctat ctagtttgga tagtgagagc
    22141 tggttggcta cttggaaaaa tgctcatgac caagcggaaa tgcaccgtgc ctacgaacgt
    22201 tatgtgattg aggaggccat tcttttatca agcacaagac aggttggttt gatggcatga
    22261 tgacgacagg ttgctctatg ggagcctatc atgcactcaa tttcttcctc cagcatccag
    22321 atgtctttac caaagtgatt gctctcagtg gtgtttacga cgcacgtttc tttgtcggtg
    22381 attactacaa cgatgatgct atttaccaaa actcgccagt agattatatt tggaaccaaa
    22441 acgacggctg gtttattgac cgttaccgtc aggcagagat tgtgctgtgt acggggcttg
    22501 gagcctggga acaagatggt ttgccatcct tttacaagct caaagaagcc tttgacaaga
    22561 aacaaattcc agcctggttt gctgaatggg gacatgatgt cgcccatgac tgggaatggt
    22621 ggcgtaaaca aatgccttat ttcctcggta atctctattt ataaaaggag ttacctatga
    22681 attaccttgt tatttctccc tactatccac aaaactttca acagtttacc atcgaactag
    22741 ctaataaagg catcacagtc ttgggaattg gtcaagagtc ttacgagcaa ttggatgagc
    22801 ccttgcgcaa tagcttgacc gagtattttc gtgttgataa tcttgagaac atagatgaag
    22861 tcaaacgtgc agttgctttt ctcttttata aacatggtcc aattggccgc atcgagtctc
    22921 acaatgaata ctggcttgag ctagacgcaa cactcagaga acaattcaat gtttttggtg
    22981 ccaaaccaga ggatctcaaa aagacgaaat ataagtctga aatgaagaaa cttttcaaaa
    23041 aagcaggtgt tcctgtggta cctggagctg ttatcaagac ggaagcagat gttgatcaag
    23101 cagtgaaaga aatcggtctt ccaatgattg ccaaacctga taatggagtg ggagcagccg
    23161 caacctttaa acttgagaca gaagacgata tcaatcactt caagcaagaa tgggaccatt
    23221 caacccttta tttctttgaa aaatttgtca cttccagcga aatctgtacc tttgacgggc
    23281 tcgtggacaa ggatggaaag attgtcttct caacaacctt tgactacgcc tatacaccgc
    23341 ttgacctcat gatttataag atggacaatt cttattatgt gctcaaggat atggatccta
    23401 aactgcgcaa gtatggggaa gcaattgtca aagaatttgg tatgaaagaa cggtttttcc
    23461 atattgagtt cttccgtgag ggggacgatt atattaccat cgagtacaat aaccgccctg
    23521 caggtggttt taccattgat gtttataact ttgctcattc cttggacctt tatcgtggct
    23581 atgcagctat tgtcgcagga gaggagttcc cggcgtcaga ctttgaaact cagtattgtt
    23641 tggctacttc tcgccgtgca aatgctcact atgtttattc agaagaggat ttgcttgcca
    23701 aatatagcca gcagttcaag gttaaaaaag tcatgccagc tgccttcgcg gaacttcaag
    23761 gagattacct gtatatgctg accactccga gtcgacaaga aatggagcag atgattgcag
    23821 atttcggaca acgtcaagaa taagaactat cggattaagg aaattaactc ccttaatcct
    23881 tttgttttgt ctgataaaaa ataagagcat cccaacaagg tagctatcat aaaacttgtt
    23941 cgataactat ttgaagcagg attaggtggt cagaaattaa attttaatat ttcaattgag
    24001 tcatagtatt gtgtttgcgt atccttaaat cagctaaaag gatccatgac gacacctata
    24061 cgatatagtt ttcaagatac caaacaagtc tattaatatt caatgaaaat caaagagcaa
    24121 actaggaagc tagccgcagg tttctcaaaa cactgttttg aggttgtgga tagaactgac
    24181 agagtcagta tcatatacct acggcaaggt gaagctgacg tggtttgaag agattttcga
    24241 agagtataaa atattcaggt gacgcataga tatagttaat tgaagctttg tttgaaatct
    24301 gataaaataa tgatattact aagttttaaa aactaaagaa aagggaagat atgattacag
    24361 gcgaattaaa aaataaaatc gatcagctgt gggaaattct ttggacagaa ggaaacgcaa
    24421 atcctttaac aaatattgaa cagttgactt atctcttatt tatgaaagat ttggatagtg
    24481 tcgagcttgg acgtgaaagt gatgctgaat ttctagggat tccttatgag ggagtttttc
    24541 caaaagataa acctgaatac cgttggtcaa cttttaaaaa tataggagat gctcaggaag
    24601 tttatcgttt aatgactcag gagatttttc cgtttattaa aaatctcaag ggggatacag
    24661 atgatacagc cttttcacga tatatgcgag aagctatttt tcaaataaat aaacctgcta
    24721 cgcttcaaaa ggcaatttct atcttagatg tttttccaac taggggatta gatgtagatt
    24781 ttgataatga caaacaaagt attactgata tcggagatat ctatgaatat ctgttatcaa
    24841 aattgtcgac cgcaggtaaa aatggacagt tccgtacacc tcgtcacatc atcgatatga
    24901 tggttgagtt gatgcaaccg actatcaaag atatcatctc agatcccgct atgggttctg
    24961 ctggcttctt agtatctgct agccgttact taaagcgtaa gaaagatgaa tgggaaacca
    25021 atacagataa tatcaatcat tttcataatc agatgtttca tggaaatgat acggatacga
    25081 ctatgttgag acttggggcg atgaacatga tgctacatgg agtagaaaat ccacaaatca
    25141 gttaccttga ctcgctgtct caagataatg aagaagccga taaatatact ttggttttag
    25201 caaatcctcc ttttaagggc tcacttgact acaattcaac ctctaatgac cttcttgcaa
    25261 ccgtaaaaac caaaaaaaca gaattactct ttctttctct tttcttgcga actttaaaac
    25321 caggtggacg agcagcagtt atcgtacctg atggtgtcct ttttggttcg tctaaagctc
    25381 ataaaggaat tcgtcaggaa attgtagaga atcataagct tgatgctgta atctcaatgc
    25441 ctagtggtgt gttcaagcct tatgctggag tttcaactgc cattctcatc tttacaaaaa
    25501 ctggtaatgg tggtactgac aaagtctggt tttacgatat gaaagcggat ggtttaagtt
    25561 tggatgataa gcgacaaccg attagcgaca atgatattcc agatattatc gaacgctttc
    25621 atcatcttga aaaagaagca gaacgtcaga gaacggatca atctttcttt gttccagttg
    25681 ctgagataaa ggaaaatgat tatgatttgt ctatcaataa atataaagag attgagtatg
    25741 aaaaagttga gtatgaacca acagaagtca tattaaagaa aatcaatgat ttagaaaaag
    25801 aaattcaagc tggcttggct gaattggaaa aattactcaa gtagggaggt ggctgtatga
    25861 aaaaagtgaa gttgggggaa gtcttatctc taaaaaaagg caagaaagcc actgtacttg
    25921 ctgaacaaac aactctaagc caacgttata ttcaaataga tgatttaaga aataataata
    25981 atttaaaatt cactgaaagt ttaaatatga ctgaagcact cccagatgat attctgatag
    26041 catgggatgg agctaatgca ggaacagttg gttatggatt atcgggagct gttggtagta
    26101 caattacggt cttaaaaaag aatgagcgat acaaagaaaa aattatatca gattacttgg
    26161 gagtcttttt ggaaagtaaa tcgcagtatt tacgagatca ttcaacaggt gcaacaattc
    26221 ctcatttaaa caagaatata ttacttgatt tacaattaga attgctaggt atcgaagaac
    26281 aagagaacat tatctgtatt cttaatacga ttaaaaggct tattactaaa agaaaatttc
    26341 agttagatga actaaacttg ctcgtcaaat cccgatttaa cgagatgttc gaagagtatc
    26401 ctgactcagt attcttagat acatatataa aagaactaag agcaggtaaa agtttagctg
    26461 gtgaggaaaa taataagaat aaagtattaa aaacaggagc tgtatcgtac gattatttta
    26521 attcatctga agtaaaaaat ttaccaattg actatattcc cttagatgaa cataaagttg
    26581 aaatagggga tgtaattatt agtcgtatga acacttctga actagttggg gctgctgggt
    26641 atgtttgggc tatcaatagt gataatatat atttgccaga tagattatgg aaggttattc
    26701 tgaatgatcg agtaaatcca gttttcttat ggaagcttat tacaaatgag aaaacaaaat
    26761 taaaaattaa gagaatttcc tctggaacta gtgggagtat gaaaaatatt tcgaaatcac
    26821 aattgttaca aattagagtt cccttccccc ctctagccct ccaaaatgag ttcgcagact
    26881 ttgtagccct ggtcgacaaa tcacaattgg caatccaaaa atctctggaa gaacttgaaa
    26941 ctttgaagaa atctctgatg caggagtatt ttggctgata ttctgccatt gtaattacgg
    27001 taatgatttg ttataatact tcaaaggagg aaatcagatg gtagtaaaaa caagaaaaca
    27061 aggaaattca atcaccatta cgattccaag tgaatttaat attccaagtg gtgttaaata
    27121 cgaagcgaaa ttgttaccaa gtggtgagat tatctttact cctgaagaat tggggcagca
    27181 ggtttcttat gtatctgatg atgcctttga cttaaattta gataaaatat ttgacgaata
    27241 cgacgatgtt ttcaaagctt tggtggaaaa atgacaatct atttgacaga aaagcaaatt
    27301 gaaaaaataa atgctttagc aattcaacgg tattctccaa atgagaaaat tcaaacagtt
    27361 agtccttctg ccttaaatat gattgtgaac ttaccagaac aatttgtctt tgggaagcct
    27421 ctttatccaa caatttttga taaagcaacg atactatttg tccaattgat aaagaagcat
    27481 gtttttgcta atgctaataa aagaactgct ttcttcgttt tggtcaaatt tttacaatta
    27541 aacggctatc gtttttctgt aacggtagaa gaagcagtaa aaatgtgtgt aaccatcgca
    27601 gtagaagctt taactgatga aaaaatgaca agctactcca aatggatttc tgaacattct
    27661 gttagagaaa aggtcaaaaa gtaacctagt atgctggatt tgaatgagca caagaaaata
    27721 aatgaacaga caatattaga attctgtaat gcagaaactg atattgtctc tttttattga
    27781 tgaataagaa agtgagaaat tatggaatca aaagttacaa ttatcatgca agaaatgtta
    27841 cctcttttaa ataatgaaca attactagcg ttgagagaga gtttagaaca tcatctagta
    27901 gacggaaaaa agcagcagaa gtattcgaat aataacctgt tgcaactatt tattaccgcc
    27961 aagcaggtag agggctgtag ctcaaaaaca attcgttatt atcagaggac gattgaaaac
    28021 ttgtttaatg ctattaaaga gtctgtgaca caactcacaa cagatgattt aaggagttat
    28081 ttagcaaatt accagtctga aaaggattgt agtaaggcaa atttagacaa tattaggcgt
    28141 atattgtctt ctttttttgc ttggcttgag caagaggaat atatcattaa aaatcccatt
    28201 cgacggataa agaaaattaa gactgagcaa aatgtgaagg aaacttatac tgatgaacat
    28261 ttggaaatta tgcgtgataa ctgtgaaaat ttgagagatt tggcaataat agacctacta
    28321 gcatcgacag gtatgcgtgt aggggagctt gtacagttga atcgttcaga tattgatttt
    28381 gaaaacagag agtgtgttgt ctttggtaaa ggaaagaagg agagaccagt atattttgac
    28441 gctcgtacga aaattcattt aagaaattat cttaacgaca gaaaagatag tcaccctgct
    28501 ctttttgtaa cgctagttgg aaaagtccag aggcttggaa ttgctggtgt agagattcgc
    28561 ttaagaaagt taggagacaa actcggcata caaaaggttc acccacataa gttcagaaga
    28621 actttagcga ctaaggcaat tgataaaggt atgcctatcg aacaagtcca aaaactgcta
    28681 ggtcatagca agattgacac aaccttggcc tatgccatgg tcaatcaaaa taatgtcaag
    28741 cattcacacc aaaaattcat ctcttaaaag caaatcccga tattccgagg aggtggttgc
    28801 atgaagaaag tgaaattagg gcaagtggct acttttatca atggctatgc ttttaaacct
    28861 caagattggt catctgaagg gaaagagatt atcagaatac agaatctgac taaaacttca
    28921 aaagggataa attattattc gggtacgatt gataaaaaat acattgttga agctggtgat
    28981 attcttattt cgtggtcggg tacattaggg gtttttcagt ggtgtggaag atccgcagtg
    29041 ttaaatcagc acatttttaa agtggttttt gataagatag atatagataa atcgtatttt
    29101 aaatatgttg ttgaaaaagg tttgcaagat gctgtaaaac atactcatgg ttccacaatg
    29161 aaacatttaa ctaaaaaata ttttgataat attatggttt catatacaaa tttgagagag
    29221 caacaaagaa ttgcaagtga gctggatttg ttaagcaagc tgattttaag gagacaagaa
    29281 caacttgaag agcttaatct acttgtaaaa tcccgattta acgagatgtt tggggaaaat
    29341 aaaatatttg aaagcattga taacttattt gatattatag atggtgatag gggcaaaaat
    29401 tatcctaaat cagatgagtt gtttagtgag gagtactgtt tatttttaaa tacaaagaat
    29461 gttactaaaa acggattttc attcgataca aagcaattta tcactaaaac aaaggataaa
    29521 ttacttcgaa aaggcaaact tgagcgttat gatatagtct tgacaacaag aggtactgtt
    29581 ggaaatgtag cgtactacga tgaattaata aaatataaac atttacgtat aaattcaggt
    29641 atggtaatat tacgtcccaa gacaccaaat ctaaatcaga aatttattat ccatgtttta
    29701 aggaataata attatagtcg agtgatatca ggaagtgctc agcctcagtt accaattaca
    29761 aaattaaaaa aaatacttct ccccctcccc ccactagccc tccaaaatga gttcgcagac
    29821 tttgtagtcc aggtcgacaa atcacaattt gcttgtgaga tagctataaa agtgtggaga
    29881 aatagcttga aatttagtat aatatagcta aactatttgt ttaaagtgag aaaaaaatgg
    29941 gaaattttag ctttctttta aaaaatgacg aatatgaatc tttttcaaaa ccttgcattg
    30001 aagctgagaa tatgattgct acatcaactg tggctactgc ctttatggcg cgtcgtgctt
    30061 tagagcaggc tgtccattgg atatatagtc acgattcata tttagaagct ccctatcgtg
    30121 ctactctatc ttctttagta tgggatgatg attttaggga tatcgtagat tctgaactcc
    30181 acaagcagat agttctgttg attcggtggg gaaaccatgc tgctcatggt ggtgaaatta
    30241 aggaacgaga agcgatttta gctttgcatc atttgtatca gtttgttaat tttatcgatt
    30301 attgttacag caatgagttt gtggagcgtt attttgatga gaagtgctta ccactttcag
    30361 caaacatcaa ataccgagaa actccacaat ctatgataaa gttacaagac agtttaccag
    30421 aactgcctga ttttcatgaa cagatggctg ctcagtccgt agaagttcaa gagacttata
    30481 ctgaaaaacg tgagactgca gcgcaacggc aagatgtgcc tttccatatt gatcaattat
    30541 ctgaggcaga gacaagaaag ctctttattg atatcgatct ccgtttagca ggatggatat
    30601 ttgaagaaaa ctgtcgtgtt gagatagccg ttgatggtct caagcacggt tcaggaattg
    30661 gttactgtga ctatgtactt tatggtaaaa atgggaaaat tttagcgatt gtggaggcta
    30721 aaaaagcctc tgtcaatcca gaagtagggg aagtacaggt caaagaatat gctgaagctt
    30781 tggagaaaca tatcggctat cagccaattt gctttattac aaatgggttg aagcactata
    30841 tacttgatgg tccgaaccgc cgccagattg caggctttta ctctcaagaa gaattgcaat
    30901 tagtgatgga tagacgtcat cttcaaaaac cgcttgagga tatttctagt aaaattaggg
    30961 acgatatttc cgggcgtcac taccaaaaac atgccattgc aagcgtttgt gaagctttct
    31021 ctgatcatcg tagacaggca cttttggtta tggcaactgg ggcggggaaa actcgtacag
    31081 cagtttctct agttgatatc ttatcacgtc ataactgggt aaaaaacgtt ctcttcttag
    31141 ccgatagaac ttccttggtt aagcaagcat atgattcgtt tagaaaatta ctcccagatc
    31201 tttccgtttg taacttctta gaagataaag aaggagctca atcaagtcgc atggtctttt
    31261 caacttatcc gaccatgatt ggagcgatta gtggtcaaga agaagtaaat caacgccctt
    31321 tcactgttgg gcattttgac cttatcataa ttgacgaatc tcaccgttct atttatcaga
    31381 aatacaagtc catttttgat tattttgatg caagaattgt aggcttaaca gctactccgc
    31441 gtcaagattt agataaaaac acctatggat tctttaattt ggagaatggg gttccaacat
    31501 atgcatatga tttggaagag gctgttaaag acggatattt agtagcctat cattctatcg
    31561 aaaccaaact gaaactacct acggatggtc tacattatga tgatttgtcc gaagaagaaa
    31621 aggaacattt tgatagcaaa tttgaagaca atagctgtga aaaagatatt gatgggagtg
    31681 tatttaattc ctttattttc aataaaagta cagtagaaat tgttttaaat gaactcatga
    31741 caagaggaat tcagacagcc tcgggtgatg aaattggtaa aactattatt tttgctaaaa
    31801 atcatgatca tgcggaatat atcagaggta tttttaacaa ccgctatcct gaaaaaggga
    31861 gcgactatgc tcaggtgatt gattatagta ttaagcatta tcagaccttg attgatgatt
    31921 ttaaaattaa ggagaagtat cctcaaattg cgatttctgt cgatatgtta gatacaggta
    31981 ttgatgtacc agaggttgtt aatttagtct tcttcaagaa agtacgctct aaaactaagt
    32041 tttggcagat gattggtcga ggaacccgtc tatgtaaaga tttatttgga cctgagcagg
    32101 ataaggaaaa cttcttggta tttgattatg gggacaattt tgattatttt cgtgcagatc
    32161 caagagatgg agagggtcgt cacattgttt cgctgactca gcgtttattt aatatcaaag
    32221 tggacttgat tcgagaactt cagggactcc aataccaaga agatcagttt gcgagagcat
    32281 accgtcagca gcttgtctcg gaacttcaag gtcgtataga gagcttaaat gagttggact
    32341 tcagggttcg tatggtttta gatacagttt atagctatag gaaattggaa agttggcaga
    32401 atctaactgc tgttacaagt gaaaccattc aaaaaaatct ctctccgctt ttatttgatg
    32461 aagataaaga agatgagatg gcgaggagat ttgatttgtg gttgcttcat attcagttgg
    32521 ggcaactgac agctaaatct tccactgttc atatttccca agtgatgaag acggctagag
    32581 ctctttctgc tattggcaat atcccgcagg tttttgagca ggctgaaatt atcaggaaag
    32641 tacaggagcc tgaattttgg aaagaagtta acttgtctga tttggaaaaa attcgtcttg
    32701 ctattcgaga tttattacag tttttggata aaacagaccg taaaccctac tatgttaact
    32761 ttgaagatcg tatactctcc actgttcacg agaccacagc atttttgcag gtcaacgatc
    32821 ttcggtctta caatgaaaaa gttgagcatt atttgaaaac tcatctggat gaggagtcca
    32881 tttctaagct ataccataat aaaaagttga catctgatga tatgcttgca cttgaaaaat
    32941 tgctttggga aaaattaggt agtaaagcag actaccaaag tcattatgaa aataaggcaa
    33001 ttccgagatt ggttcgtgag attattggct tagatagaga gtctgccaat cgtatttttt
    33061 ctaaattttt gtcggatgag aatcttaatg ccaggcagat ttcatttgta aaattgattg
    33121 tagactacat tgtagaaaat ggttttttag agacgaaagt gttaacgcaa gagccgttta
    33181 aatcttatgg ttctgttcaa ctactcttcc aacaccaact accagtactt cgtaatattg
    33241 ttcaaatcat tgaacttatc aataatcgag ctggagaagc ggcttaaatt ctaaagtgat
    33301 tgccatgctg agactcattt aaaattaaaa agagtagaaa tttatgctat atatgagaag
    33361 ttttattagg aagaatgtca tcgttttcct agaatacagt atcagttgtt aagtggttga
    33421 taaatttcaa agtagatact tgtaccacga tgtttgttga tcgagttatt aacaaaagag
    33481 ctactttgat tttaaagaaa tagaaaacaa aaagccgagc aagaattcaa ttgcaggaga
    33541 aaatgaaata atactcaatg aaaatcaaag agcaaactag gaaactagct gcaggctgct
    33601 caaaacactg ttttgaggtt gcagatggaa gctgacgcgg attgaagaga ttttcgaaga
    33661 gtataaatct tcctaggata aagcaaaacg catagtatca agggttttca acacttgata
    33721 ctatgcgttt tctgatgtta aagactttct accaggtttt ttaaaagcat aattgttagt
    33781 tgtagtcatt tattattctt caaagaaaaa tggtggggcg aattttttca gttcttcaaa
    33841 gcacttttga gcagtatctg catcttcaca gatgataaga cagacatcat taccacaaag
    33901 ggtagcgata gcgtcaggga agctcaaagt atcaatgata gaaccaaagg attgagccag
    33961 tccaggaagg gtttttagta ggacttggtg ttgaactggg cgcatccaga caagggcgtc
    34021 ttccatgtag agttcgagac gtttttccca ttttgagatg gaaccattgt taagaacata
    34081 ataagcgcta tcttcttcgc ggacttttga taggttcata tttttgatgt cgcgtgagag
    34141 ggttgcctgg gttacttgaa tgtcgttctc agcaagaagg gcttgcaact cagcctgtgt
    34201 atgaatcttg ttttttgtga taagagcgcg tataagttgg tggcggtgtt ctgatttatt
    34261 cataataatg taactccttt tagcaaggta aggtaagcat ggactgagcg aggtcgacag
    34321 tcaagtggta gtctgtattg tcacggatgg tgatttcaaa gtcagtagta tagaggacta
    34381 aacggagagt gtctccttct tttagcttgt aaatagttgg ctgcagttca aattgaacgt
    34441 ccatccattc atctgcagta atatcctcta ctaacagtaa atcatttcta ttttgtaaat
    34501 taaggtaacc ttttgtcacg actcgttgtg cctctggtct aaatggcaat tcacagagat
    34561 tttccaacat gtgatagcga ccgttgtcaa tggttctagc acttaaaata gctggataag
    34621 gttgtaggta tttcttttgc ccaaattcta gcagttgggc agataagagc cccttgtttg
    34681 tactggattt gatacgaaga ttgagctgag cgcgaccgtt taggtggaga tctttagtca
    34741 caggaaggtt aatagtaatc tgattggctt tcccttgata gagctctgta ttgaaggttt
    34801 ggtatgtctt accatagcgc tcaaaatcct tatctgggta ctggttttga atagcttgct
    34861 cttcttgacc aagtgagaag gtttcacagt tttcttgccc accgaagtta tcaagtgata
    34921 accaagtctg tggagctgta ttgtcctgcc agataacagt aggaagttga aagtctgttt
    34981 cctgtcctag taatttcttg gtcaataagg catttatgga ctcacggaag tcaattgatt
    35041 gccaattgtt catgtaaaca tgggcaccat tatggaaaaa gagatgcttg tgtatatgag
    35101 taggaagagc atggaacatc tggtaaacat gaagtggttt gacattccaa tcctgagaac
    35161 catgagtaaa gacaacctct gcctttactt tatgggcatt gagcagataa ttgcggtcat
    35221 gccaaaactg attgtagtcc ccagtttttc ggtctagctg agctttcact ttttctaagt
    35281 cagcttggtg agcttcattg ccacggatat agtcgccagc taagagatta cgagaatagg
    35341 ttaactcagc aagggagtca aagtcctcac ctggataacc acctgggcta gtcaccagac
    35401 cgttttcacg gtagtagttg taccatgatg aaattcctgc ctcggcaatg ataacttcta
    35461 aaccatcgac tcctgtagtc gcaagaccat tggacatggt acctagatag gaaagtcctg
    35521 ttgtagcaac ttttccgttt gaccaatcag ccttgacttg acgctggcgc gtgtgatcag
    35581 taaaggcacg gcaacgaccg ttaagccaat cgatgacatt tttataagcc tcgatttgct
    35641 ggtagtctcc attagtcatg aaacctgtcg agtctttggt accaacacct gagacataga
    35701 gattggcaaa gcctctcgga aggaagtagt cgtttagtgt atagctagag ttgatgtgag
    35761 ttagcttttc ctcagcctct gctataagct cagctttacc ttggggttgg acgagattta
    35821 gttgaggttt ctctagctca atcttgtgag gaagcttaac ctcaagctcg ccctccatct
    35881 tgtagagagc cttgtcacta gccttgtcat tggttccctg atgataaggg ctggctgtca
    35941 tgatggcagg gatttttcca tcaaaacgag ggcgaataat gctaaccttt actaggtctg
    36001 atagcccttt ttggtcagta tcgacacgag actcaacgta aacgacttca cgaatgacat
    36061 cctggttaga aaaagtagcc aaactcttgc cgttaaagta gtggtagtca ttatcctccg
    36121 gaataagacc atcactaaca agttggtcga taagagtatt tccttttttg gtgcgagtat
    36181 tgagtaactg atagagattt tcaatcaagt caccatatat aatgggaaat ccagtttctt
    36241 tacgaaaaac gtcactatct tcgaagtcaa ccaaataaga aaagcctaaa agttgaaaag
    36301 caacagtata aaaaatatct gctgtcagtt catcttctga ttgaaaaaat gtcagcaggt
    36361 ctgttttttt atcagctgct aggatagaaa gtgggtagtt ggtgtcttga taagtgaaaa
    36421 agaaacgacg taaaaaggtt tcaagtgagt ctttgtgatt ggctgtattt tgtaaatcaa
    36481 agccacattt ttttagttca gataagacat tttcttttgg aaaattgata taactatatt
    36541 gattaaaacg catagaacct ccatatagaa tgacagttaa ggttattata tcaaaaaaaa
    36601 agcagaaagg gaattgttaa cttcaaaagg aaataatcca ataaaaatga ataaagtact
    36661 aaattcaata tagagaacag agtaacaata agaataaata gatagggtat aaaagttcta
    36721 ggagatttat attatatgct ttctattttt atatacaata tagtataaat ataaaaatga
    36781 tgacaaaaat acaaatgaat agaaaataaa ttagtaagct gatgaaattt ttctcaagag
    36841 aagccattta taggtgaaaa tggtataata tagtgagaag gatagaggag aagtgtaaat
    36901 tgatcgcaca actagataca aaaacagtct atagttttat ggaaagcgtc atttcgatcg
    36961 aaaagtatgt gagagcagct aaagaatacg gctacactca tttggctatg atggatattg
    37021 acaatcttta tggcgctttc gactttctag agattacaaa aaaatacggc attcatcctt
    37081 tgctagggct tgaaatgaca gtgtttgtag atgatcaggg agtgaatttg cgctttttag
    37141 ctctatctag tgtgggctat cagcagttga tgaagctttc gacagccaag atgcaggggg
    37201 agaaaacttg gtcagtcctg tcccagtacc tggaggatat cgcggtcatt gtgccttatt
    37261 ttgatagagt tgagtcgtta gaactaggct gtgattacta tataggggtt tatccagaaa
    37321 cactagcaag cgaatttcat catcctatct tacctcttta tcgggtcaac gcttttgaaa
    37381 gcagggatag agaagttctt caagttttaa cagcgattaa agaaaatcta ccgctcagag
    37441 aagttccctt gcgttcgaga caagatgtct ttatatcagc aagttcttta gagaaactat
    37501 tccaagagcg ttttccgcaa gctttggaca atttagaaaa gcttatttca ggcatttctt
    37561 acgacttgga tactagtctg aaactgcctc gttttaatcc agctagacca gcagtagagg
    37621 agttgagaga gcgtgctgaa ctggggcttg ttcagaaggg gttgactagt aaagaatatc
    37681 aagatagact agaccaagaa ttgtctgtta ttcatgatat gggctttgat gattatttct
    37741 tggttgtttg ggatttgttg cgttttggac aatcgaatgg ctattatatg ggaatgggaa
    37801 ggggttctgc agtaggcagt ttggtttctt atgccttaga catcacgggg attgacccag
    37861 tagagaaaaa tctgattttt gaacgctttc ttaatcgtga acgctatacc atgcctgata
    37921 ttgatattga tatcccagat atttatcgtc cagattttat cagatatgtt ggtaataaat
    37981 atggtagtaa acatgcggca caaatcgtta ctttttcaac ctttggagcc aagcaagctc
    38041 ttcgagatgt cttgaaacgc tttggtgtgc cagagtatga attatctgca attactaaga
    38101 aaatcagttt tcgtgacaat cttaagtcgg cctatgaggg aaatctccag tttcgtcagc
    38161 aaatcaatag taagttagaa taccaaaaag cttttgagat tgcttgcaag atagagggct
    38221 atccaaggca aacctctgtc catgcggctg gtgttgtaat tagtgaccaa gatttaacca
    38281 actacattcc tctaaagtat ggtgatgaaa ttccactgac tcagtatgat gctcatggag
    38341 ttgaggctag cggacttttg aagatggact ttctgggact acgaaatttg acctttgtcc
    38401 agaagatgca agagttgctt gctgaaacag aaggtattca tctgaaaatt gaagaaatcg
    38461 atttagaaga caaagaaacg ttagctttat ttgcctctgg taatacaaaa ggtatctttc
    38521 aatttgagca accaggtgcc attcgtctgc ttaagcgtgt gcaaccagtc tgttttgaag
    38581 atgtcgtcgc gactacttct ctaaatcgac cgggtgctag tgactatatc aataattttg
    38641 tggcaagaaa gcatgggcag gaagaagtga ctgttctgga tccagtactg gaggatattt
    38701 tggctccaac ctacggcata atgctctatc aggagcaggt tatgcaggtt gcccagcgac
    38761 ttgccggatt tagtcttggg aaagccgata ttttgcgtcg ggctatgggg aaaaaggatg
    38821 cctctgccat gcatgagatg agggcttcct ttattcaagg ttcattagaa gctggtcata
    38881 ctgtggaaaa agcagagcag gtctttgatg ttatggagaa gtttgcaggt tatggtttta
    38941 acaggtcaca cgcctatgcc tactcagcct tggccttcca gttggcttat ttcaaaacgc
    39001 attatccagc cattttttat caggtcatgt taaattcttc caacagtgat tacttaatag
    39061 atgcacttga agcaggtttt gaagtagcct ctctatccat caacaccatt ccctatcacg
    39121 ataaaattgc caacaaggcc atctatctag gtttgaaatc cattaaagga gtcagtaatg
    39181 atttagctct ctggattatt gaaaatagac cttattctaa cattgaagat tttatagcta
    39241 aattacctga gaattatctg aaacttcctc tgctagaacc tttggtaaaa gttggtcttt
    39301 tcgattcatt tgaaaaaaat cgtcaaaaag tatttaataa cttagctaat ctatttgaat
    39361 ttgtgaaaga gttgggaagt ttgtttggag atgctattta tagttggcag gaatcggaag
    39421 attggacgga acaagaaaaa ttttatatgg aacaagagct tttagggata ggtgtcagca
    39481 aacatccact acaagctatt gcaagtaagg ctatttaccc gattacccca atcggaaatt
    39541 tgtcagaaaa tagctatgct attatcttgg ttgaagttca gaaaataaaa gtgattcgta
    39601 ccaaaaaggg tgaaaatatg gccttcttac aggcagatga tagtaagaaa aaattggatg
    39661 tcactctctt ttcagactta tatcgtcagg ttggacagga aataaaagag ggagccttct
    39721 actatgtaaa aggaaaaata caatcacgtg atggccgtct gcaaatgatt gcacaagaaa
    39781 taagagaagc agttgctgaa cgcttttgga tacaggtgaa aaatcatgaa tcggatcaag
    39841 aaatttcacg cattttagaa caatttaaag gcccaatccc agtcatcatc cggtatgaag
    39901 aggaacagaa aaccatcgtt tctccccatc attttgtagc taaatccaat gaattagagg
    39961 agaaattgaa tgaaatcgtt atgaaaacga tttatcgcta aaaatacgga aaatagaaga
    40021 attttcaacg taaatgtggt ataatcagta agaatgttaa aagaaaaagg agcataacca
    40081 atatgaaacg tattgctgtt ttgactagtg gtggagacgc ccctggtatg aacgctgcca
    40141 tccgtgcagt tgttcgtcaa gcaatttcag aaggaatgga agtttttggt atctatgacg
    40201 gatatgctgg tatggttgcc ggtgaaattc atcccctaga tgcagcttca gtaggggaca
    40261 tcatttctcg tggtggtact ttccttcact cagctcgtta cccagagttc gctcaacttg
    40321 aagggcaact taaagggatt gagcaattga aaaaacacgg aattgaaggt gtagttgtta
    40381 tcggtggtga cggatcttac cacggcgcta tgcgtttgac tgaacatggc ttcccagcta
    40441 ttggtcttcc aggtacaatc gataacgata tcgttggtac tgactttaca atcggttttg
    40501 acacagcggt tactactgcc atggacgcta tcgataagat tcgtgataca tcatcaagtc
    40561 accgtcgtac ttttgtaatc gaagttatgg gacgtaacgc tggtgatatc gctctttggg
    40621 ctggtattgc aactggtgct gatgaaatca tcatccctga agcaggcttc aagatggaag
    40681 atatcgtagc aagcatcaaa gctggttatg aatgtggtaa aaaacacaat attatcgtct
    40741 tagctgaagg tgtgatgtca gcggctgaat ttggtcaaaa acttaaagaa gctggagata
    40801 caagcgacct tcgtgtaaca gaacttggac atattcaacg tggtggttct ccaactgcgc
    40861 gtgaccgtgt tttggcgtca cgtatgggtg cacatgctgt taaacttctt aaagaaggta
    40921 tcggtggtgt tgcggttggt attcgtaacg aaaaaatggt tgaaaatcca attcttggta
    40981 ctgcagaaga aggggcattg tttagcctta ctgcagaagg taagattgtg gttaacaacc
    41041 cacacaaagc cgatattgag ctatctagct tgaataagag cttgtcataa tttacaattt
    41101 agttaataag accaaaaagg tcatatatat aaaggagtca caaaaatcat gaacaaacgt
    41161 gtaaaaatcg ttgcaacttt gggtcctgcg gtagaaatcc gtggtggtaa aaaattcggt
    41221 gaggacggat actggggtga aaaacttgat gttgaagctt cagctaaaaa cattgctaaa
    41281 ttgattgaag ctggtgctaa cacattccga ttcaacttct cacacggcga ccaccaagaa
    41341 caaggtgagc gtatggcaac tgttaaactt gcggaaaaaa ttgcaggtaa aaaagttggt
    41401 ttccttcttg atacaaaagg acctgaaatc cgtacagaat tgttcgaagg tgaagctaaa
    41461 gaatattcat acaaaactgg tgaaaaaatt cgtgttgcaa ctaaacaagg aatcaaatca
    41521 actcgtgaag tgattgcgtt gaacgttgct ggtgctcttg atatctatga tgatgttgaa
    41581 gttggtcgtc aagttttggt tgacgatggt aaactgggtc ttcgtgtggt tgctaaagat
    41641 gatgcaactc gtgaatttga agttgaagtt gaaaacgatg gtatcatcgc taaacaaaaa
    41701 ggtgtgaaca tccctaacac taaaattcct ttcccagctc ttgctgaacg cgataacgac
    41761 gatatccgtt tcggtcttga acaaggtatc aacttcatcg caatttcatt cgtacgtact
    41821 gcaaaagatg tgaacgaagt tcgtgcaatc tgtgaagaaa ctggaaacgg acatgttcaa
    41881 ttgttcgcta aaatcgaaaa ccaacaaggt atcgataact tagatgaaat catcgaagca
    41941 gctgatggta ttatgattgc tcgtggtgat atgggtatcg aagtaccgtt cgaaatggtt
    42001 ccagtttatc aaaaaatgat tatcaagaaa gtcaatgctg caggtaaagt tgttatcact
    42061 gcaacaaaca tgcttgaaac aatgactgaa aaaccacgtg caactcgttc agaagtatca
    42121 gatgtattca acgctgttat cgacggaact gacgctacaa tgttgtcagg cgagtctgca
    42181 aacggtaaat acccactcga gtcagtaact acaatggcta caatcgacaa gaacgctcaa
    42241 gctcttctta atgaatacgg acgtcttgat tcagattcat ttgagcgtaa ctctaagaca
    42301 gaagtaatgg cttctgctgt taaagatgct actagctcaa tggatatcaa attggttgta
    42361 actcttacta agacaggtca tactgcacgt ttgatttcta aataccgtcc aaatgctgac
    42421 atcttagcat tgacatttga cgaattgaca gaacgtggct tgatgttgaa ctggggtgtt
    42481 atcccaatgt tgacagatgc tccatcttca actgacgata tgttcgaaat cgctgaacgt
    42541 aaagcggtag aagcaggtct cgttgagtca ggcgatgata tcgttatcgt tgctggtgtg
    42601 ccagtaggag aagctgttcg cacaaacaca atgcgtatcc gcacagtacg ttaagaaaaa
    42661 tataaaaacc tatcatatcc agctttagag cttgtgtgat aggctttttg tatagagggt
    42721 aagaaatagg caaaactttc ataatggatt gatactcttc gaaaatctct tcaaaccacg
    42781 tcagcgtcgc cttaccgtat atatgttact gacttcgtca gttctatcta caacctcaaa
    42841 gcagtgcttt gagcaacctg cggctagctt cctagtttgc tctttgattt tcattgagta
    42901 tgaaataaga tatgcacaaa ttgattagaa agtcaaatga atttctacaa atgttttagc
    42961 aatcgtaatg tacttgtcta gattcgatct gatatatttt cgatttaatg atatggtatt
    43021 taaaacctcc aaagtagctt actccattct tttacttacg tgagtgtaga tgttatttac
    43081 tgttttagcg tttttgtgtt ccactctaac cattatagca ttcttctcag ctagtgtact
    43141 aaggagtgtg tgcctgaaaa tatgggaact aaggggctgg tttatcggtt tctctagttt
    43201 agtatttgcc ttttgcaaag tgatcttaaa tgcctttctc taaatttaca tatcactatt
    43261 gtttaacaaa atctaatcta ttttaggtca cttattcttt ttttgaaatg tagaatgaac
    43321 tttttcaaag tttttcgaat cttttaaaat ctgtttgctt tatatcgcca ttctcccccc
    43381 ttttttaatt ctccctatat agcctgacag ctttcccgat ggtacgaata tggttgcttt
    43441 cgtctaggtg gatgtcgggg tattcgggat tgagtttttt tgaggcagcc ttggcggagt
    43501 ttcttgacat agttagtgcc gtctacttgg aagatgccga tggtattata gtcaatctgt
    43561 ggggtattct tgataaatag gtagtcgctg tttcttatct ttggctccat ggacttgctg
    43621 acgacataag cgattgggtc gtagtcgtct gggataatgg aaactccata tctaaatcgt
    43681 tgtcctgcat cgagcggcta cctgcagaga taaactacct aacacgagag taagtagtct
    43741 gtctgtagtc gtccagtctg atgattttta cgatacttcg tttttctgat catacagttg
    43801 cctctcggca taggtcagaa ctttaccttg tctgggtggt tcccgttggt cgtagataga
    43861 ttggatatcg ctaggagaat ccttttgaac tggaggaaag agggcatcga tcaagctact
    43921 gaatacttta actaagtcaa atatagtatt tttcttagta gacctaaccc ttttttcata
    43981 atttctaatg gtgtttttac ttatacctat cttagtaccc aattcttatt gagtccaacc
    44041 attactagtc tatattgttt tatagttgat tgagtttgga atagtacgct gtagctgcta
    44101 aaacatttct agaaattaat ttgactttcc taatagagtt gttcatatct tatttcaatc
    44161 tattatgttt ttcacctcta acaatcgcaa tctcttcttt atccatgaat gaaatcgctt
    44221 tctatttttg taagtaaagc ataacacgaa atccacgaaa atgaaaacct ttgttgtgtt
    44281 ttcgtaaaaa atttgttgac agagcacgaa acgcctacta taattaaatc aagcttaagg
    44341 aaataacaaa aaaacaaatc ggagggaaag acgatgaaca cattaaatga gaaagtaatc
    44401 aatatctgta aagcaatagt taaagaaact ttaatccaag acatttagga gaaagaaatg
    44461 aagaaatcaa gaaaattagc cacattagga atctgttcag ccttgttttt aggcttggct
    44521 gcttgtcaac aacaacatgc tacttctgag gggacgaatc aaaggcaaag cagttcagcg
    44581 aaagttccat ggaaagcttc atacaccaac ctaaacaacc aggtaagtac agaagaggtc
    44641 aaatctctct tatcagctca cttggatcca aatagtgttg atgcattttt taatctcgtt
    44701 aatgactata ataccattgt cggctcaact ggcttatcag gagatttcac ttcctttact
    44761 cacaccgaat acgatgttga gaaaatcagt catctctgga atcaaaagaa gggcgatttt
    44821 gttgggacca actgccgtat caatagttat tgtcttttga aaaattcagt caccattcca
    44881 aagcttgaaa agaatgacca gttgcttttc ctagataatg atgcgattga taaaggaaag
    44941 gtctttgatt cacaagataa ggaagagttt gatattctat tttcgagagt tccaactgag
    45001 tcaactacag atgtcaaggt tcacgctgaa aagatggaag cattcttctc acaatttcaa
    45061 ttcaatgaaa aagctcgaat gctgtctgta gtcttgcacg acaatttgga tggcgagtat
    45121 ctgtttgtag gccacgttgg ggtcttagta cctgctgatg acggtttctt atttgtagag
    45181 aaattgactt tcgaagagcc ctaccaagcg attaaatttg ctagtaagga agattgctac
    45241 aagtatttgg gcaccaagta tgcggattat acaggcgagg gactggctaa gccttttatc
    45301 atggataatg ataagtgggt taaactttaa tagtgttatg atatgacagt aatcttagga
    45361 tttttatttc tatttttgat aagttggtgg gttatttcaa tcgttaatag ccagaaacca
    45421 cgcaaaaatg aaacatttat tggctatgtc caaagatacg atattgatgg gaatggctat
    45481 caatcattac cgtgtcctca gaactgagag gagttcttga aatcgtaaca ccactttgaa
    45541 caagagttac tt
//
LOCUS       DAVKNL010000013        43340 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVKNL010000013 DAVKNL010000000
VERSION     DAVKNL010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 43340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43340
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.013"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(73..1035)
                     /locus_tag="VW411_000648"
     CDS             complement(73..1035)
                     /locus_tag="VW411_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEU9793428.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            1101..2489
                     /locus_tag="VW411_000649"
     CDS             1101..2489
                     /locus_tag="VW411_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9793429.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAADKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            complement(2524..2976)
                     /locus_tag="VW411_000650"
     CDS             complement(2524..2976)
                     /locus_tag="VW411_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU9793430.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            3138..3432
                     /locus_tag="VW411_000651"
                     /pseudo
     CDS             3138..3432
                     /locus_tag="VW411_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3588..4802
                     /locus_tag="VW411_000652"
     CDS             3588..4802
                     /locus_tag="VW411_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9793431.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            4947..5081
                     /gene="rpmH"
                     /locus_tag="VW411_000653"
     CDS             4947..5081
                     /gene="rpmH"
                     /locus_tag="VW411_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEU9793432.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            5365..6258
                     /locus_tag="VW411_000654"
     CDS             5365..6258
                     /locus_tag="VW411_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEU9793433.1"
                     /translation="MVAQDPKAQDSTKLTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATPEY
                     KLENTPGGDKGGNTGSSDANANEGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIAMVTVPKRPVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALAS
                     LGLVVATSGFALLGRKTRRRK"
     gene            6563..7336
                     /locus_tag="VW411_000655"
     CDS             6563..7336
                     /locus_tag="VW411_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU9793434.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIE
                     HALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHSKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            7336..7896
                     /gene="rnmV"
                     /locus_tag="VW411_000656"
     CDS             7336..7896
                     /gene="rnmV"
                     /locus_tag="VW411_000656"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEU9793435.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINAQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMMVIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            7968..8867
                     /locus_tag="VW411_000657"
     CDS             7968..8867
                     /locus_tag="VW411_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9793436.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLDYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMPQIIEKEAYSINDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTQIENYFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALREERDFSLAESFYQSSKTFAQ
                     LIGDEFLVKKLTEEWQEDVKKYL"
     gene            9136..11166
                     /locus_tag="VW411_000658"
     CDS             9136..11166
                     /locus_tag="VW411_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9793437.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETRFTYATYGEGKLPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSVYYQGLFTDYL
                     QNFSSESVEVTSQKHYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEKGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            11168..11809
                     /locus_tag="VW411_000659"
     CDS             11168..11809
                     /locus_tag="VW411_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9793438.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            11802..12110
                     /locus_tag="VW411_000660"
     CDS             11802..12110
                     /locus_tag="VW411_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793439.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFERMFRENC
                     "
     gene            12241..13113
                     /gene="rsmA"
                     /locus_tag="VW411_000661"
     CDS             12241..13113
                     /gene="rsmA"
                     /locus_tag="VW411_000661"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEU9793440.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            13115..13993
                     /gene="rsgA"
                     /locus_tag="VW411_000662"
     CDS             13115..13993
                     /gene="rsgA"
                     /locus_tag="VW411_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEU9793441.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            14004..14660
                     /locus_tag="VW411_000663"
     CDS             14004..14660
                     /locus_tag="VW411_000663"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU9793442.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTSVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGEVNERVQTLRKQLD
                     "
     gene            14623..15285
                     /locus_tag="VW411_000664"
     CDS             14623..15285
                     /locus_tag="VW411_000664"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEU9793443.1"
                     /translation="MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLE
                     EDLPLALAVGDFDSVTEEERQVIQKGAQYFVQARPEKDDTDLELALLTIFEQNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMHQIEIEDGQNLITYCPEGISQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            15287..16543
                     /locus_tag="VW411_000665"
     CDS             15287..16543
                     /locus_tag="VW411_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEU9793444.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVVVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFARDIRNKYI
                     APPRTTNFGVLFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            16533..17474
                     /locus_tag="VW411_000666"
     CDS             16533..17474
                     /locus_tag="VW411_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEU9793445.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            17741..18568
                     /gene="purR"
                     /locus_tag="VW411_000667"
     CDS             17741..18568
                     /gene="purR"
                     /locus_tag="VW411_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEU9793446.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            18635..19885
                     /locus_tag="VW411_000668"
     CDS             18635..19885
                     /locus_tag="VW411_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEU9793447.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     ISLDFINLSGGIGVNYHPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEVVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            20011..20805
                     /gene="pflA"
                     /locus_tag="VW411_000669"
     CDS             20011..20805
                     /gene="pflA"
                     /locus_tag="VW411_000669"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEU9793448.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKQLMDTESYQDYMKRVHG"
     gene            complement(20880..21806)
                     /gene="yidC"
                     /locus_tag="VW411_000670"
     CDS             complement(20880..21806)
                     /gene="yidC"
                     /locus_tag="VW411_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU9793449.1"
                     /translation="MKSIKRFALSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(21884..22162)
                     /locus_tag="VW411_000671"
     CDS             complement(21884..22162)
                     /locus_tag="VW411_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEU9793450.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYPDFKIAN"
     gene            22196..22936
                     /locus_tag="VW411_000672"
     CDS             22196..22936
                     /locus_tag="VW411_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU9793451.1"
                     /translation="MTIITSKANSVVKNTKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFTLENYRDQLATFSQTVWVSEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            22992..23675
                     /locus_tag="VW411_000673"
     CDS             22992..23675
                     /locus_tag="VW411_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEU9793452.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYLLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            23787..24779
                     /locus_tag="VW411_000674"
     CDS             23787..24779
                     /locus_tag="VW411_000674"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEU9793453.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            24844..25383
                     /gene="rsmD"
                     /locus_tag="VW411_000675"
     CDS             24844..25383
                     /gene="rsmD"
                     /locus_tag="VW411_000675"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEU9793454.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEVGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAERELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            25373..25861
                     /gene="coaD"
                     /locus_tag="VW411_000676"
     CDS             25373..25861
                     /gene="coaD"
                     /locus_tag="VW411_000676"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEU9793455.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKAVKHLGNVKVVSSHDKLVVDVAKRLGATCLVRGLRNASDLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACYVPESILEEIRNEK
                     KD"
     gene            25845..26882
                     /locus_tag="VW411_000677"
     CDS             25845..26882
                     /locus_tag="VW411_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU9793456.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            26940..27170
                     /locus_tag="VW411_000678"
     CDS             26940..27170
                     /locus_tag="VW411_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEU9793457.1"
                     /translation="MVQLLFTLSSHMFFIYVSFYLLKDLVRWEKVLKVTDDNTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            27194..28477
                     /gene="murA"
                     /locus_tag="VW411_000679"
     CDS             27194..28477
                     /gene="murA"
                     /locus_tag="VW411_000679"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU9793458.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            28470..28658
                     /locus_tag="VW411_000680"
     CDS             28470..28658
                     /locus_tag="VW411_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU9793459.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            28697..29521
                     /gene="endA"
                     /locus_tag="VW411_000681"
     CDS             28697..29521
                     /gene="endA"
                     /locus_tag="VW411_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEU9793460.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMPSAPNSPKTNLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            29750..31081
                     /locus_tag="VW411_000682"
     CDS             29750..31081
                     /locus_tag="VW411_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEU9793461.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPIIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(31279..31349)
                     /locus_tag="VW411_000683"
     tRNA            complement(31279..31349)
                     /locus_tag="VW411_000683"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31315..31317),aa:Cys,seq:gca)
     gene            31765..35376
                     /gene="rpoB"
                     /locus_tag="VW411_000684"
     CDS             31765..35376
                     /gene="rpoB"
                     /locus_tag="VW411_000684"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU9793462.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TSEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVIAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            35409..39086
                     /gene="rpoC"
                     /locus_tag="VW411_000685"
     CDS             35409..39086
                     /gene="rpoC"
                     /locus_tag="VW411_000685"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU9793463.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            39197..39610
                     /gene="ndk"
                     /locus_tag="VW411_000686"
     CDS             39197..39610
                     /gene="ndk"
                     /locus_tag="VW411_000686"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEU9793464.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEIIQNVVHGSDSEESAKREIALWF"
     gene            complement(40556..41188)
                     /locus_tag="VW411_000687"
     CDS             complement(40556..41188)
                     /locus_tag="VW411_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9793465.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLVYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            complement(41190..41957)
                     /locus_tag="VW411_000688"
     CDS             complement(41190..41957)
                     /locus_tag="VW411_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9793466.1"
                     /translation="MSQKMNHLDVGEFVLLLPEHLRSEEEHYKSVFEDDLTSRISSQD
                     ERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQSILFWIDAVQ
                     NYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVMLAGAVLGIAT
                     SILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLGFVASVFLQVE
                     IGVAFLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
     gene            complement(42008..43340)
                     /locus_tag="VW411_000689"
                     /pseudo
     CDS             complement(42008..43340)
                     /locus_tag="VW411_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699970.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
BASE COUNT        12710 a         8377 c         9841 g        12412 t
ORIGIN      
        1 aaactgacga agtcagtaac catacatacg gtaaggcgac gtgacgtggt ttgcagagat
       61 tttcgaagag tattagcctt ccatatagtc tttcaaagcc tgtcctgtta gtccggcatt
      121 gagggcgatg aggagtttca agcgagcttt ttgggcgttg agttctttaa caaagaaaac
      181 gcctgctttt tgcaactgta cgcccccacc ctggtatgca taaacaggct cggcaatacc
      241 gttaaagcat cgtgatacca gagcgactgg aattcctttt tgcagaaggc tttctaattt
      301 ttgagccgtt tctttgggaa tattaccagc tccgaaggct tggataatca aaccgtccaa
      361 gtgttctaaa tccagcatat caatcagctc atctgtcata ccagcataag ccgagatgat
      421 agggactaaa ccttgtatgt gatcaaggtc aaagcgaaca cgaggttcag ctgttttgaa
      481 gtagaggatt tcctgtttca tgatgagacc aagggggcca tgtgttggag tctggaaggt
      541 gctgacatta gtcgtatgtg ttttggtgac atacttggca gcgtggattt catcgttcat
      601 aacgaccaaa actcctttgt cagcagccct gtcatcgctg gccactcgta aagcacttag
      661 gtaattataa acaccatcac taccgagctc attggagcta cgcatggctc ctgttagaac
      721 gataggcata tggggaactt ccatggtatc aaggaaatag gctgtttcct ctaaagtatc
      781 ggttccgtgt gtgatcacca ctccatcgta gttatctgct tcctctttaa ttttctggta
      841 gaggaccagc atatgtttgg gtttgatatg gggacttgga aggttaaaaa agtccaaggc
      901 gtggacttgg attccttcaa gtgggttgga cacatggttc atgggattat ctgaactcgt
      961 cacaacagcg ccagaagcat cggcctgcat ggaaatagtt ccacccgtat gtaaaacaag
     1021 gattttctta ggcatgattt actcactctt tctgaaatgt ggtataatca ttgtatcaca
     1081 aaaggggaga ttgggatagg atggaagtca aagctgtttt ttttgatatc gatggaacct
     1141 tggtcaacaa tcgcaagagt gttttgaaat ccactaagga tgcgattaag attgtcaaag
     1201 aacaaggggt actagttggc gtagcgacag ggcgaggacc tttttttgtt aaggaattga
     1261 tggacgattt ggatctggat tttgcggtaa cctataatgg ccagtatatc tttagtaaag
     1321 acagagtctt gttcacgagc cctatttcca agttacattt gcgccatcta attagctatg
     1381 ctaaaaaaga gggcacggag attgctctag ggaccaagga tgccatgttg ggttctaaaa
     1441 tcatgtcctt tggtctgggt tctttttccc aacgaattag tcgcttcgtt ccctctgttt
     1501 taactcggac ggtgagtcag tcttttaatc gcatggtcag caaggttgtt ccccaaaagg
     1561 aagaggacct gcttcacctg atgaatcagc ctatctacca agttttgatg ctgatgacac
     1621 cagaagaatc tgagaaggcg gcagctgatt ttgaagactt gaaattgaca cgtagcaatc
     1681 cttttgcatc ggatgtcatc aatcagggaa attctaaatt agaaggcatt cgccgagttg
     1741 ggaaagaata tggctttgac ctcaaccaag tcatggcttt tggtgactca gataatgatc
     1801 ttgaaatgct ggctggcgtt ggtatgtcgg tcgctatggg aaatggtagt agcagtgtca
     1861 aggaagctgc caagcacatt accactagca atcaacaaga tggaatccac aaggccttgg
     1921 aacattttgg tgttttgtct tcagaaaaag tctttgtcag ccgtgactat catttcaata
     1981 aggtcaagac cttccaccac atgatggatg aacgaaccca agaagaacct cgagcttggg
     2041 atttagaggg tgcaacccat agggctggtt ttaaaataga agaattggtg gagtttgttc
     2101 gagcagccag tccttctgaa gaagattttg gtcaagctgt atcgcaactt catcaggccc
     2161 ttgataaggc agcagataaa gtagccaaga agacacctgc tcagcaagat ttgatagggc
     2221 aagtggatgc cttgattgac acgctttact tcacctacgg cagttttgtc ttgatggggg
     2281 tggacccaga acgtattttt gatattgtcc atcaggccaa tatggggaaa atttttccag
     2341 atggcaaggc tcattttgat ccagtgaccc ataaaatctt aaaaccagat aattgggaag
     2401 aaaaatatgc tccagaacct gctattaaaa aggaactgca gcgccagctc aaggcttacg
     2461 aacgccataa agagagaaat aaatcgtaaa caaaaaggag ctaggggctc cttttctttg
     2521 tgattataag gttttttctt gttctctcac aaccagcaaa tcgaccttag catggcggag
     2581 tatgtattca gatgaagagc cgaccaagag gcgttcaaag gcgttgagac cagttgcgcc
     2641 aacgaggatg aggtccactt cctcggcatc tggaatagta cgtgctagca gggtctttgg
     2701 atttcccatt tcaatgacga tatgaacatc tgctacccca gcatctttag cacgtttttc
     2761 gtactctttc atcagacttt cagcgtcgac ttggagttct tcgtaaactt cagcatcaaa
     2821 ggtggatacg ctttggagag cgcgtgtgtc aatgacatgt gcgatggtga gtttagcgtc
     2881 gtttcgtaga gcagaatgaa ctcccttgac aaaagccaag tccgcttcct tagaaccatc
     2941 gattgcgacc atgatatttt ggtaacgttg tgccataatg aaacctcctg aaattttctt
     3001 actataattg taaacctttt cactatagaa gtaaagcaaa aaaagtattt ttcaaaagaa
     3061 tattagggct taaactctat ataaatatat gataaaataa aagaaaggat agcaggaaag
     3121 aagtgaagga agggagaatg atgaaaaact cttttcaaaa atcaaatttc ctttattatg
     3181 gaattatcct agtttcagtc ttggtagaag tgatcatgat ctgtcaatta gagagtctgg
     3241 taccgcttct ttatccaagt tttattggat ttttagtttt tcatgtactc taccatctaa
     3301 tcttatttct agttgcaaaa cgaagtgggc gttgggatta cttgatgata tgggggcttt
     3361 tcctcatgtt taatcttctt tatgactcct ttctaggctt gcttttttct aggtttatct
     3421 tttggtatgt gatgtgtctt tgcaaaatac ctgctatctc tacgttctta acctcgcctt
     3481 tagcaaggtt tttcttctat acgaaactag taacacattt cagtaaactt gtcgcattct
     3541 ctttctagtg gtataatgtt actatcctga tgaattgagg aaacaagatg aaagaatata
     3601 acaagtctag taagttagag catgttgctt atgatatccg tggtcctgta ttggaagaag
     3661 ccatgcggat gcgagcgaat ggagaaaaga ttttacgtct gaatacagga aatccagcag
     3721 aatttggctt tacagcgcca gacgaggtca ttcatgactt gattatgaat gcgcgtgata
     3781 gtgaaggcta ttctgactcc aaagggattt tctcagcccg taaggccatc atgcagtatt
     3841 gccagctgaa gaaattccca aacgtagata ttgatgatat ttaccttgga aatggtgtca
     3901 gtgagttgat tgtcatgtcc atgcaggggc ttttggacaa tggggatgag gtcttagtgc
     3961 caatgccaga ctatcctcta tggacggcgg ctgtcagcct agctggagga aatgccgttc
     4021 actatatctg tgatgaagct gtggaatggt acccagatat tgacgatatc aagtcaaaaa
     4081 tcacttccaa taccaaggca attgtcctta tcaatccaaa taacccaact ggagcccttt
     4141 atcctaagga actcttgctg gagattattg agattgcccg tcaaaacgat ttgattatct
     4201 ttgcggatga aatctatgac cgcatggtaa tggacggaca tgtgcatacg cctgtggcga
     4261 gcttggcacc agatgtcttc tgtgtcagca tgaatggtct gtcaaaatcc caccgcatag
     4321 caggtttccg tgtgggttgg atggtcttgt ctggccctaa gactcatgtt aagggctata
     4381 tcgaagggct caatatgctg tccaatatgc gcctttgctc taacgttttg gcccaacaag
     4441 tcgtacaaac ttccttgggg ggtcaccaat cagtcgatga attgcttctt cctggtggac
     4501 gaatctacga gcaaagaaat ttcatctata atgccattca agatattcca ggtttgtctg
     4561 ccgttaaacc caaggcgggg ctctatatct tcccaaaaat cgaccgcaat atgtaccgta
     4621 tcgatgatga tgagcagttt gtccttgatt tcttgaagca ggaaaaggtt ctcttggttc
     4681 atggtcgagg ctttaactgg caggaaccag accacttccg tatcgtttac cttcctcgtg
     4741 ttgatgagtt agcccaaatc caagaaaaga tgactcgttt cttgaaacag tatcgtagat
     4801 agggcttgca ttcgaaaaag ctggaaacat ttgcctagag ctattgacaa aagtgaaaga
     4861 atcagataga atagtaaagt atgtttagta actgatcact ttatagccgg ctaaacgaat
     4921 acaaaatcta tgaggaggta ttcatcgtga aacgtactta tcaaccaagt aaacttcgtc
     4981 gtgcgcgcaa acacggattc cgtaaccgta tgtcaactaa aaatggtcgt cgcgtattgg
     5041 cagctcgtcg tcgtaaagga cgcaaagttt tggctgcata atccaaacga attctatcaa
     5101 aaatcagtag gaactcgagt ctactgattt ttatttttgt aaaaaagttc agtagatgca
     5161 aatggattcg gaagcgatgt tacagtagat tgaaactaga atagtacacc tctgtttcta
     5221 aaacattgtt agaaatcgat ttgactgtcc tgatcgattt gtcctgttat tattttattt
     5281 tactataaag ttgaagtagg tggagatggt acagcaacaa tcgtctttaa agatggttca
     5341 gctattacaa ttccaggaaa tcaattggta gcacaagatc caaaagcaca agatagcact
     5401 aaactgactg ctgaaaaatc aactgttaaa gcacctgctc aaagagtaga tgtaaaagat
     5461 ataactcatt taacagatga agaaaaagtt aaggttgcta ttttacaagc aaatggttca
     5521 gcattagacg gagcgacaat caatgtagct ggagatggta cagcaacaat cacattccca
     5581 gatggttcag tagtgacgat tctaggaaaa gatacagttc aacaatctgc gaaaggtgaa
     5641 tctgtaactc aagaagctac accagagtat aagctagaaa atacaccagg tggagataag
     5701 ggaggcaata ctggaagctc agatgctaat gcgaatgaag gcggtggtag ccaggcgggt
     5761 ggatcagctc acacaggttc acaaaactca gctcaatcac aagcttctaa gcaattagct
     5821 actgaaaaag aatcagctaa aaatgccatt gaaaaagcag ccaaggacaa gcaggatgaa
     5881 atcaaaggcg caccgctttc tgataaagaa aaagcagaac ttttagcaag agtggaagca
     5941 gaaaaacaag cagctctcaa agagattgaa aatgcgaaaa ctatggaaga tgtgaaggaa
     6001 gcagaaacga ttggagtgca agccattgcc atggttacag ttcctaagag accagtggct
     6061 cctaatgctg ctcctaagac aacaagtgca ccgcaagcaa ctgcaggaac aatgcaagat
     6121 gttacctacc agtcacctgc tggcaaacaa ttacctaaca caggttcagc atcaagtgca
     6181 gcacttgcta gtcttggtct agtggtggca acaagtggtt ttgctttgct aggaagaaag
     6241 actagacgta gaaaatagaa cagctagaaa attctattct ctacttaaag ttagattata
     6301 agggggattt tgagaagtca tcaatcctag tgatgggtga gaaaagtgag aacccaagat
     6361 aatcacatac tttagctgaa taggaatatt ctatcaatgt agccaatctc ttctgtctct
     6421 aactgtggaa taggagatgg gcaatatcgg atagaaaaga tagcagaata gctctctatt
     6481 gaagagagga ggggaaaccg aaaaattagg tgcccctcct cttttttggt ataatagaag
     6541 atagaaaacg aggttagaag agatgatttt tgatacacat acacacttga atgtagaaga
     6601 atttgcaggt cgtgaggcag aagaaattgc cttggctgct gagatgggtg tgacacagat
     6661 gaatattgtt ggttttgata aaccgacgat tgagcatgcc ttggagttgg tagatgagta
     6721 tgagcagctc tatgcgacta ttggttggca tcctacagaa gctggtactt atacagagga
     6781 agttgaggct tacttgttgg ataagttaaa acattccaag gttgtggctt taggtgaaat
     6841 tggcttagat taccattgga tgacagcgcc caaagaggtg caggagcagg tttttcgccg
     6901 tcagattcag ctatctaagg acttggattt gccttttgtt gtccataccc gtgatgcgct
     6961 ggaagatacc tatgagatta tcaagagtga gggcgttggt cctcgtggtg gtatcatgca
     7021 ttcattttca gggacgcttg agtgggcaga gaagtttgtg gatcttggta tgaccatttc
     7081 cttctcagga gtggtgactt ttaagaaggc aactgacctc caagaagcag ctaaagagtt
     7141 acctttggac aagatgttgg tggaaacaga tgcgccttac ttagcacctg tacccaagcg
     7201 tggtcgtgaa aataaaacag cctatactcg ctatgtggtc gactttatcg ctgacttgcg
     7261 tggtatgacg acagaagagc tggcggtagc aacgactgca aatgcagaac gaatttttgg
     7321 actggacagc aagtaatgaa agagaaaatt tctcaagtta tcgtggttga agggcgtgat
     7381 gatacggtca atctcaaacg ttatttcgat gtggagacct atgagactcg aggttctgcc
     7441 atcaatgctc aggatataga gcggattcag cgcctgcacc aacgtcatgg agtcattgtc
     7501 tttacagacc cagattttaa tggggaacgg attcggcgca tgatcatgat ggtcattcca
     7561 acagttcagc atgcctttct caagcgagat gaagctgttc ccaagtccaa gaccaagggg
     7621 cgttctctgg gaattgagca tgccagctat gaagacctga aaacggctct agctcaagtg
     7681 acagaacaat ttgaacatga gagtcagttt gacattagtc gtagcgattt gattcgcctt
     7741 ggttttctag caggggcaga cagccgtaag cgtagagaat atctcggaga gactctccga
     7801 atcggctatt ccaacggcaa gcaactcctc aaacgcctag agttgtttgg ggttactttg
     7861 gcagaagtgg aagaagctat gaaatcttat gagtaggaaa gatgtagccg ttacaatttt
     7921 ttaagtttca cagtattttt cgaagcaggt agaagaggag gcgtctgatg ttaattggtc
     7981 aaaaaattaa agagattcgg atagaaaaag gaattagtcg tccagatttt tgtggagatg
     8041 agcaagaact gacagttcgt caactgtcgc gaattgaaag tggagcttcg caaccgagtt
     8101 tgcccaagtt agactatatt gctcgccggc taggagttcc agtttatagc cttatgccgg
     8161 atttttcagc tcttccttct gcttatttag aattgaaata ccagatttta cgtgaaccaa
     8221 tctatggtaa agaagaggag tacgataaga aggaagcgtg tttggaagag atttataaaa
     8281 catactttga taatcttcct aaagaagaac aattagcatg tgaagtattg caggcgtgtt
     8341 tggatacttc tagaactaga aggcctgaat atgcagagtt aatacttgag gaacatatgc
     8401 ctcagattat agaaaaagaa gcttattcaa taaatgatat gttgttgatt cgtttgtttt
     8461 tttatcaaat gctcattaga aaagatcttg ccaaatttat aaatcaaatc gaaaagctaa
     8521 tgctctttct tttggaacag aagaaggtaa ctcaaataga gaattacttt ataattagag
     8581 atactcttat ttcaggaatg tgttgtcttg aaaaggtagg agtaactgat tgttttaatg
     8641 attatctatc gtgtttacaa gaaattatgg ataaaactca agattatcaa aagaaacctc
     8701 ttgtatttat gtttttgtgg aagcaagcat taagagaaga aagagatttt agtttagctg
     8761 aatcatttta tcagtcttct aaaacatttg cgcagctaat tggagatgaa tttctagtaa
     8821 agaaattgac agaggaatgg caagaggatg tcaaaaaata tttataaaca tagtgaatca
     8881 gtgacaaaga tgtccttgtc ctcgtatcaa aacagttcta aagttcgtct ttagggatgt
     8941 ttttttagat ataagctaaa aatgacacga aatggttaga ttttaaggac attgatgtcc
     9001 ttgatagttc ttgaaaaaac ggatataatg tatttgaaga tgttgctagc agaaagaact
     9061 taataagctt cttaacatcg cagtgactgt ggtcaccttc tcacgaagtg accaagtaac
     9121 tttttggagg aaatgatgaa acgtttattt attttgattt caatggtatt agtatcgctt
     9181 tatatggtga taacttccgt tgaccatcga gaagagattt tatttggtaa ctatccttct
     9241 gttgatgtga caggaatgat gataaatcaa ccagtagcta gtcgcgaaga ggtgacagag
     9301 gctttgagtc acttggcggt agagcacaat agtctcattg ctcgtcgaat cgttgagcca
     9361 aatgaagctg gagaaacacg ctttacctat gccacttatg gtgagggaaa gcttccagaa
     9421 ggtctgacca tttcctccaa ggagagtgca gaaacgagtg atttattagg gtcttacttg
     9481 attgtatcag gaagtttgga tggagtgagc ttacagacca ccttgaaaga gcttggttat
     9541 caaggctttg tttcgaatgg agaagatcca ttttcgatag tcttactatt gacggccacc
     9601 cctatggtgc tactgagttt agctattttt ctgctgacct ttatgagtct gaccctgatt
     9661 tatcggatca aatcccttcg tcaggcaggg attcgcttaa tagctggtga gagcttgttt
     9721 ggagttgctc tcagaccagt gttagaagat gtgagacagc ttatctgctc agtgctggta
     9781 tccagtcttt tgggattggg gattctctgg tatcaaggtg ccttgtttat ggcaacggtg
     9841 caactggtca tcattgctct tctactttat ggattgacct tggcagggat ttctacctta
     9901 ctaagtgtcg tctatctact tggtttacag gaaaatagtc tggtggatct attgaaaggg
     9961 aaactccctc tcaaacgtat gatgacattg atgatggtgg ggcaactctt agctgtattg
    10021 gtggtcggat cgagtgcgac agctctccta ccccactacc gtgaaatgca ggaaatggag
    10081 agagctagca ataaatggag ccagtcctca gaccgttacc gtctatcctt tggttggtct
    10141 agtgcatttg ccgatgaaga aggaacgcgt aaggataatc gtgagtggca gacatttact
    10201 gaagaacggt tagccaatac agactctttt tatattatga gcaatgttga caatttctca
    10261 gatggagcag aagtggacct agatggcaat cgtctcagtg actacacacc gtcagggaat
    10321 gttatctatg tctcaccgcg ctatctgata gaagaaaaga ttaccgtttc ttcagagttt
    10381 atggacaaga tgcaaaactt gtctgaggga gagtttgggc tgatcttgcc tgagagcttg
    10441 cgagagcagt ctgtctacta ccaaggattg tttacagatt acctgcaaaa cttttcatct
    10501 gaaagtgtag aagtgacgag tcagaaacac tacctcccac aggtaaggct agcttttaca
    10561 gaaacaggac aggaacgttt cctctataat gatgggtaca agacaacacg ccagtaccta
    10621 aaagatccga ttattgtagt tctaacgccg caagcgactg gaacaagacc tgttgcaggg
    10681 atgttgtggg gaactacggc taatagtgcc ttgaaactag atcgatatgg agacagcatc
    10741 acagctctaa aagagaaagg tctgtatcac aaggtttctt acttggtaaa aagccagcta
    10801 ttttttgcca aggtactaaa tgacaaacgg gtggagtttt actctctcct tattgggacg
    10861 attttgaccc tgtctacggc tatcttgtta tttgattcca tgaatcttct ctattttgag
    10921 cagttcagac gggaacttat gattaaacgt cttgctggta tgacaatcta tgagcttcat
    10981 ggcaagtatt tactggcgca aggaggagtt ctcttgcttg gcctagtcct atctagtatt
    11041 ttgacaagag atggtttgat tagcgctcta gttgtagctt tgtttacgct taacgccctc
    11101 ttgattttag taaggcagga caaaaaagaa gaagctggta gcatggcagt attgaaagga
    11161 aaataagatg attgatattc aaggattgga aaagaaattt aatgaccgcg cgattttctc
    11221 tggtttgaat ctcaagctgg agaagggcaa ggtttatgcc ttaatcggaa agagtggaag
    11281 cggaaagacg acgctgctga atatcttggg aaagctagaa aagatagatg gtggaagggt
    11341 tctctatcag gggaaagatt taaaaaccat tcccactcgt gagtattttc gagaccagat
    11401 gggctatctc tttcaaaatt tcggcctctt agaaaaccaa tcaatcaaag aaaatttgga
    11461 tttgggtttt gttggtcaga aaatctcaaa agtagaacgt ttggaaaggc aagtgggggc
    11521 tttagaaaaa gttaatctag ggtatttgga tttagaacaa aaaatctata ctttatctgg
    11581 gggagaggcc caacgagttg cccttgctaa gactattttg aaaaatccac ccttgatttt
    11641 ggcagatgaa ccaacagcag ctcttgatcc tgaaaattca gaggaggtta tgaatctctt
    11701 ggtggatttg aaagatgaaa atcgaattat catcattgcg acccataatc ccctagtctg
    11761 gaataaggct gatgaaatca ttgatatgag gaaacttgct catgtgtgaa aaaatccgta
    11821 ttcgcagggt atctgattat cctagtgcca gaggtggttt agaagatatc ctcatcatgg
    11881 aaaatatgac caatcatctc cttttggttc aaatccgagt gcatggctat ttgcttgatt
    11941 ttgctagtat tgaagggcaa aggcaaaagc attatcgttt gaaaaattta cctcagacgg
    12001 ttgaactgac agtggatgat gtggaggagg atgtggattt gaccctacct gaaaatcgaa
    12061 gttatcaaga agctgatttt tttgaacgca tgtttcgaga gaactgctaa ggccactttt
    12121 aaagatttcc aagactatct ttcttcatga ggaaagatag ttttttggta tgattttcat
    12181 tcccaaaata caaggggaat gtgttacaat agtagtaaca gataatagaa aagagaatag
    12241 atgagaattg cagattatag cgtgaccaag gcagtgctgg agcgtcacgg ttttaccttt
    12301 aaaaagtcct ttgggcaaaa ttttttgacg gataccaata tccttcaaaa aattgtggat
    12361 acggctgaaa ttgatgatca ggtcaatgtc atcgaaatcg ggccaggtat tggtgccttg
    12421 acagaatttt tggctgagcg tgcagcccaa gtcatggctt ttgagattga ccaccgtttg
    12481 gtgccaattt tggcagatac cctgcgtgat tttgataatg tgaccgtagt taacgaagat
    12541 attctcaagg ttgatttggc gcaacatatc cagaatttta aaaatcctga cctgccaatc
    12601 aaggtagtgg ctaatttgcc ttactacatc acgacgccta ttctcatgca cttgattgag
    12661 agtggcattc ctttttgtga gtttgtggtc atgatgcaga aagaagtagc ggaccgcatt
    12721 tcagcccagc ctaacaccaa ggcttacggt agcttgtcta tcgccgtgca gtattacatg
    12781 acagccaagg ttgcctttat cgtgcctcgt acggtctttg tgccagcgcc aaatgtggat
    12841 tcagccatct tgaaaatggt gcgtcgtcca gagccagccg tagcagtaga agatgagaac
    12901 tttttcttta aggtttccaa ggctagtttt acccatcgcc gcaagacctt gtggaataac
    12961 ttgacaggtt actttggtaa gactgaagag gtcaaggaca agctgaccaa ggctttggac
    13021 caggcaggct tgtcaccaag tgtgcgtggg gaagctctca gcttggcaga atttgccggt
    13081 ctagcagacg cacttaaagg gcaaggactc taagatgcag ggacaaatca ttaaagcctt
    13141 ggcaggtttc tactatgtgg agagtgatgg ccaggtttat caaacacgcg cgcgtgggaa
    13201 tttccgtaaa aaaggccata ccccttatgt tggggactgg gtagatttct ctgccgagga
    13261 aaattcagaa ggctatatcc tcaaaattca cgaacggaaa aacagtctgg ttcgtccgcc
    13321 tattgtcaat atcgatcaag ctgtagtaat catgtccgtc aaggaacctg attttaacag
    13381 caatttgctg gatcgtttct tggttctttt ggagcacaag ggcatccatc ccattgtcta
    13441 tatttccaaa atggatttgt tggaagatag gggagaactg gatttttacc agcagaccta
    13501 tggtgacatc ggctatgact ttgtgaccag taaagaggaa ctcctgtctt tgttaacagg
    13561 caaggttacg gtctttatgg ggcagacagg tgttgggaag tcaactcttc tcaataaaat
    13621 cgcaccagac ctcaatcttg aaacgggaga aatttcagac agtctaggtc gcggtcgcca
    13681 taccactcga gctgttagtt tttacaatct caacgggggt aaaatcgcag atacaccagg
    13741 attttcatcc ttggactatg aagtatcaag ggctgaagac ctcaatcagg ctttcccaga
    13801 gattgctact gttagccgag attgtaagtt ccgtacttgt acccataccc atgagccgtc
    13861 ttgtgccgtc aaaccagctg ttgaagaggg tgttattgca accttccgtt ttgacaatta
    13921 cctgcaattc cttagtgaaa ttgaaaatcg tagagaaacc tataaaaaag tcagcaaaaa
    13981 aattccaaaa taaggagaaa cctatgtctc aatacaagat tgctccgtca attctggcag
    14041 cagattatgc caactttgaa cgtgaaatca aacgtctaga agcaactggg gcagaatatg
    14101 cccatatcga tatcatggac agtcattttg taccgcaaat cagttttggt gcaggtgtgg
    14161 tcgagagcct tcgtcctcat agtaagatgg ttttcgattg ccacttgatg gtgtcaaacc
    14221 ctgagcatca tctggaagat tttgcgcgtg caggtgcaga catcatcagt atccatgtag
    14281 aagcaacgcc tcatattcat ggcgccctcc aaaaaattcg ttcactcgga gttaagcctt
    14341 cagtcgttat caatcctggc acatcagttg aagccatcaa gcacgtcctt catctagttg
    14401 accaagtttt agtcatgacg gttaatccag gttttggtgg gcaagccttt ctgccagaaa
    14461 ccatggataa ggtccgtgag ttggttgctc ttcgtgagga aaaaggtttg aactttgaaa
    14521 tcgaagtgga tggtgggatt gatgaccaaa ctattgctca agccaaagaa gccggtgcga
    14581 ctgtttttgt agcaggttcc tatgtcttta agggagaagt caatgagcga gtacaaactc
    14641 tcagaaaaca actggactag ggttgcagtt tttgcaggcg gaaaccgcgg tcattatcgg
    14701 acagattttg atgcttttgt tggggtggat cgaggctcgc tctgggtctt ggaagaagac
    14761 ttacctcttg ctctagcagt cggagatttt gattctgtga cggaagaaga gcgacaggtg
    14821 attcaaaaag gtgcccagta ttttgtccaa gcacgaccag aaaaggatga tacagatctg
    14881 gaattggctc tcttaaccat ctttgaacaa aatcctcagg ctcaggtcac tattttcggt
    14941 gccttgggtg gccgtattga ccatatgttg gccaatgtct ttctgcctag caatcctaag
    15001 ttggcaccct atatgcatca aatagaaatt gaggatgggc aaaacttgat tacttattgt
    15061 ccagaaggaa tcagtcagct agaacctcgt tcagactacg actatctagc ctttatgcca
    15121 gttcgggata gccagctgac tattcttgga gccaagtatg agttgacaga ggaaaatttt
    15181 ttctttaaaa aagtgtacgc ttctaacgaa tatatagata gggaagtgtc ggtaacttgc
    15241 ccagatggtt atgtggtcgt actgcatagc aaggacagga ggtaggatgg aaagtttact
    15301 tattctatta ttaattgcca atctagctgg tctctttctg atttggcaaa ggcaggatag
    15361 gcaggagaaa cacttaagta agagcttgga ggatcaggca gatcatttgt cagaccagtt
    15421 ggattaccgc tttgaccaag ccagacaagc cagccagtta gaccaaaaag atttggaagt
    15481 ggttgtcagc gaccgtttgc aagaagtgcg gattgaattg caccaaggtc tgacccaagt
    15541 ccgtcaagaa atgacagata atctcctcca aactagagac aagacagacc aacgtctcca
    15601 agccttgcag gaatcaaatg agcaacgttt ggaacaaatg cgccagacgg tcgaggaaaa
    15661 actagaaaag accttgcaga cacgcttaca ggcttccttt gagacagttt ctaaacaact
    15721 ggagtctgtc aatcgtggcc ttggagaaat gcagacagtt gcccgtgatg tcggagctct
    15781 taacaaggtt ctctctggaa ccaagacgcg agggattctg ggagaattgc aactggggca
    15841 aattattgaa gacatcatga cacctgccca gtacgaacga gaatacgcaa cggttgaaaa
    15901 ctctagtgaa cgagtggagt atgccatcaa gttacccgga caaggcgacc aagaatacgt
    15961 ctatctgcca attgactcta agtttccact ggcagattat taccgcttgg aagaagccta
    16021 tgagacaggt gacaaggatg agattgaacg ctgtcgtaag tcactcctag caagcgtcaa
    16081 gcgctttgct agggatatta ggaacaagta catagcacca cctcggacga ccaattttgg
    16141 agttttgttt gttccgacag aaggtctcta ctcagaaatc gtccgcaatc cggtcttctt
    16201 tgatgatttg agacgggaag aacagattat tgttgcagga ccaagtaccc tatcagccct
    16261 tcttaactcc ctatcagttg gtttcaagac ccttaatatc caaaagagtg ccgaccatat
    16321 cagcaagact cttgccagtg tcaagaccga gtttggcaag tttggtggta ttctggtcaa
    16381 ggcacaaaaa catctccaac atgcctctgg caatattgat gaattattaa accgtcgtac
    16441 catagctatc gagcggacgc tccgtcacat tgagttgtca gaaggtgagc ctgcgcttga
    16501 tctactccat tttcaagaaa atgaggaaga atatgaagat tagtcacatg aaaaaagatg
    16561 agttatttga aggcttttac ctaatcaaat cagctgacct gaggcaaact cgagctggga
    16621 aaaactacct agcctttacc ttccaagatg atagtggcga gattgatggg aagctctggg
    16681 atgcccaacc tcataacatt gaggccttta ccgcaggtaa ggttgtccac atgaaaggac
    16741 gccgagaagt ttataacaat acccctcaag tcaatcaaat tactctccgc ctgcctcaag
    16801 ctggtgaacc caatgaccca gctgatttca aggtcaagtc accagttgat gtcaaggaaa
    16861 ttcgtgacta catgtcgcaa atgattttca aaattgaaaa tcctgtctgg caacggattg
    16921 tccgaaatct ctacaccaag tatgataagg aattctactc ctatccagct gccaagacca
    16981 accaccatgc ctttgaaacg ggcttggcct atcatacggc gaccatggtg cgtttggcag
    17041 acgctattag cgaagtttat cctcagctca ataagagcct gctctatgcg gggattatgt
    17101 tgcatgactt agctaaggtc atcgagttga cggggccaga ccagacagag tacacagtgc
    17161 gaggtaatct tcttggacat atcgctctca ttgatagcga aattaccaag acagttatgg
    17221 aactcggcat cgatgatacc aaggaagaag tcgttttgct tcgtcatgtc atcctcagtc
    17281 accacggctt gcttgagtat ggaagcccag tccgtccacg cattatggaa gcagagatta
    17341 tccatatgat tgacaatctg gatgcaagca tgatgatgat gtcaacagct cttgctttgg
    17401 tggataaagg agagatgacc aataaaatct tcgctatgga taatcgttcc ttctataaac
    17461 cagatttaga ttaataattt aagaaaaatg agcatttttt aggataagaa tgttcgtttt
    17521 tttatgtgaa tatggtataa taagtaaaag acaaaaatga atactcttcg aaaatctctt
    17581 caaactaggg tagtatcgcc ttgtcgtatg tatatatgca ggtatattac agggtttgtc
    17641 agttctattg acaatctcaa aacagtgttt tgaaccacca gcgaccagct ttctagtttg
    17701 ctttttgatt ttttgaataa aaatggaata ggaaatagaa atgaaattaa gaagaagtga
    17761 tcggatggtt gtcatttcca actatttgat taataatcct tataaactaa ctagtctcaa
    17821 tacttttgct gaaaagtatg agtctgctaa atcatccatc tcagaagata tcgtcattat
    17881 caaacgcgcc tttgaggaaa ttgaaatcgg tcatatccag acagtgactg gggctggcgg
    17941 aggtgtcatc ttcacaccgt ctatttcgag tcaggatgct aaggaaatgg ttgaagactt
    18001 gcgtaccaag ttgtcagaaa gtgaccgtat cttgccaggt ggttatatct atctgtctga
    18061 tttgcttagc acaccagcca tcttgaaaaa tattggtcgt attattgcca aaagctttat
    18121 ggaccaaaaa attgacgcgg ttatgaccgt agcaactaag ggtgtgccac ttgcaaatgc
    18181 agttgccaat gtcctcaatg tctcttttgt cattgtgcgc cgtgacctga aaattaccga
    18241 aggttcaact gttagcgtca actatgtttc aggttcaagt ggtgaccgta tcgagaaaat
    18301 gttcctttca aaacgtagtc ttaaggcagg cagccgtgtc ttgattgtgg atgacttctt
    18361 gaaaggtggc ggaacggtca atggtatgat tagtctcttg cgcgagttcg actcagaact
    18421 ggcaggtgta gcggtctttg cggacaatgc ccaagaagaa cgtgaaaagc agtttgacta
    18481 caagtcactc ttgaaggtaa ccaatattga tgtcaagaac caagccatcg atgttgaggt
    18541 tggcaatatc tttgacgaag ataaataaga gatagaacta aaggttggaa cgattgtccc
    18601 agcctttctt tgcaaacaga atagaaggaa gcttatgaaa acaccattta tcaatagaga
    18661 agagttagaa gcgattgttg ccgagttccc gactcccttt cacttgtatg atgagaaggg
    18721 gattcgtgag aaggcaagag ccgtcaacca agctttttcg tggaacaagg gctttaagga
    18781 atattttgca gttaaggcta ctccaactcc agctattttg aaaattctcc aagaagaagg
    18841 ttgtggtgtg gactgctcta gttatgtaga gcttttgatg agccataaac tggactttct
    18901 gggttctgag attatgttct cttccaacaa cacgccagac aaggaatacg cctatgcacg
    18961 tgaattgggt gcgaccatta acttggatgc ctttgaagat attgaacatc tggagagagt
    19021 agcaggcatt ccagaaatca tctcttgtcg ttataatcct ggaggcgttt ttgaactggg
    19081 gacagacatt atggacaatc ctggggaggc taagtttggc atgaccaagg accagctctt
    19141 tgaagccttt gctatcttga aggaaaaagg agccaagact tttgggattc actccttcct
    19201 agcgtccaat accgtgaccc atctctatta tccagagttg gctcgtcagc tctttgaact
    19261 ggctgttgaa atcaaggaaa agttgggcat ttcgctagac tttatcaatc tttctggcgg
    19321 tattggtgtt aattatcatc cagaccagga gccgaacgat atcgccttga ttggtgaggg
    19381 agttcgtaag gtgtatgaag aggttcttac gtcagcaggt cttggtcagg tcaagatttt
    19441 caccgaattg ggtcgtttta tgctggcacc tcacggtgct ctagtcacaa gagtcactca
    19501 taagaaggaa acctaccgta cctatctagg tgtggatgcc tcagcagtca acctcatgcg
    19561 tccagctatg tacggagctt accatcatat tagcaacgtg acccatccag atggaccagc
    19621 tgaagtggta gatgtggtcg gttcactctg tgaaaacaat gataaatttg cagttaatcg
    19681 cgaactgcct catacagaaa tcggtgattt gctggtcatt catgatacag gtgcccacgg
    19741 attttcaatg ggctaccagt ataatgccaa attacgttct gcggaaatcc tctataccga
    19801 agaaggtaaa gcccgtcaaa tccgccgtgc agagcgccct gaggactatt ttgcaacctt
    19861 atatggcttc gattttgaag aataatctga taatagattg aaaatgaaat tgaaaaacag
    19921 attgctttct aaaaaatagg caaaaatctt gtttttcctt caagtcgtga tataataaaa
    19981 ctataaaacg ttttcaagga aggtaacgat atgtctgaag aaacaattga ttatggacaa
    20041 gtgacaggaa tggtgcattc gacagaaagc tttgggtcag tagatgggcc tggtattcgc
    20101 tttattgtct ttttgcaggg ctgtcacatg cgttgccagt attgccacaa cccagacact
    20161 tgggctatgg agtccaataa gtcacgtgaa cggacggtag atgatgtctt gacagaggcc
    20221 ttgcgctacc gtggtttctg gggaaataag ggtgggatta cagtcagtgg aggagaagct
    20281 ctcttgcaga ttgatttcct gattgctctc ttcaccaagg ctaaggaaca aggaatccac
    20341 tgtaccttgg acacctgtgc tcttcctttc cgtaataaac cacgttacct tgagaagttt
    20401 gacaaactca tggctgtcac tgacttggtt cttttggata tcaaggaaat caacgaagaa
    20461 cagcacaaga ttgtcactag ccaaaccaat aaaaatatct tggcttgtgc ccagtatcta
    20521 tcagatattg gaaaacctgt ctggattcgc cacgtgctag ttccaggatt gacagacaga
    20581 gatgatgact tgattgaact tggtaagttc gtcaagaccc tcaaaaatgt tgataagttt
    20641 gaaattctac cttatcacac catgggtgag ttcaagtggc gtgaacttgg aattccatat
    20701 tccctcgaag gagtcaaacc accaacagca gatcgcgtca agaacgctaa acaactcatg
    20761 gataccgaaa gttatcaaga ttatatgaaa cgtgtacatg gatagaaaag aagcctgatg
    20821 gaaacatcgg gcttttgact tgcaaaaaga cttagcaaat cagctaagcc tttttcttct
    20881 tatctcgaac gttgttttcc agcgttgcga tttttgtgtt ttttcttgct tgtgatagca
    20941 gttggttgtt caggggtaac gtcttttcgt ccacttggtt tagagaaagc acttgctttt
    21001 ggtgggttct tggctagttc ttcacggact tttttgcgaa gttttggacg aacgatatag
    21061 ttgacgataa actgttggag aatcatcatg aaaccaccga caacccagta aagtgtgaca
    21121 ctagctggtg agaagaggga gaagacgacg atcatgagtg ggctcatgta aatcattttc
    21181 ttgatttgtt ctctttgcat ttcatcttct actccgtgaa gtgaaaggag cgattgaaga
    21241 tagtaaagga caccagcaca ggcaaccaaa atcatacttg gagaacctag aggaatgcct
    21301 aggtagcttg cttgagcaac cccttcagta tgttgggcag caaagtagat agcagagaag
    21361 aaaggcattt gaaggaggat agggaaacat cctacaccgc caaacatgct gataccgtgc
    21421 tctttttgag cagcaaagag agcttgttgg gcttcgagtt tttcttcttg agtagtcgct
    21481 tctttgagac gcgtttggtg tggctcaagg acgtgcttga gggcgttcat cttttcagag
    21541 tgaagcgttg ccttccatga ttggtagata ccaagtggta agataatcaa gcgtacgata
    21601 atggttacga taatgatagc gacaccaaag cctagacctt tatcagtagc gaagtacttg
    21661 atggcttcag ccataggcgc tccgatcgta ttccaaataa atcctgttgg ctgacctgtg
    21721 gttttatcga cattgacaca gccagtcaag acaagcaaca tagccactcc catagccgag
    21781 agtgcaaaac gtttaataga tttcactgtt tttattcctt ctttaaaaat tatacctttc
    21841 tattctactg tttttttaca aaatatacaa tagttctgga gacctaattt gcgattttaa
    21901 agtcagggta gggaggaaag ttgctcagtt tgacatctaa gtaactgact tttgaaaaag
    21961 gtgtcggtcc ttttcggatt tcttggataa attttgccat aatagctgat gagtctgctt
    22021 gggctaagat ttccactgtg ccatcgtcgt tattccatac ccgacctgtg atgccaccga
    22081 tttcaagggc caggctataa acaccccaac gaaagccgac tccctgcacc ctaccttggg
    22141 caatcattct aaccttttgc ataccaaacc cctttgattg tgttataata tttctatgac
    22201 tattataacc tcaaaagcca attctgtggt aaaaaatacc aagaaattac accaaaaaaa
    22261 ataccgcaag tcagcctatt tgattgaagg ctggcacttg tttgaagaag ctgttcaagc
    22321 tggagtgacg attgagaaaa tctttaccct agaaaattat cgagatcagt tagcaacttt
    22381 ttcgcaaact gtctgggtgt cagaggatat tttgctagat ttggcggatt ctcagactcc
    22441 acagggaatt gtggccgtgg ttcaaaaaga agaagtagga caagctgatt tgagtcaggg
    22501 caagttcttg tttttggaag atgtgcaaga tcctggtaat gtaggaacta tcattcgaac
    22561 tgcggatgca gcaggtttta caggagtgat tgtttcagat aagtcggcag acatctacag
    22621 tctcaaaacc ctacgttcca tgcaaggtag tcatttccat ctgcccattt accggatgac
    22681 tagtcaagcg cttcttgacg aaactaaaaa ggtagctatc ccagtgttag caacaaccct
    22741 atctaaagat tctgttgatt acagagaact gccttctata gaaaattttg tactagttat
    22801 gggaaatgag ggtcaaggaa ttagtcccct tatggctgaa agtgcagacc agttggtcca
    22861 tattagcatg aaggggcagg ccgagagttt gaatgttgcg gttgcagccg gtattttaat
    22921 cttccattta agctaatttt aacttttttt gttataatca aggaaagatg ttcacagaaa
    22981 aggagaaatg gatgaatcac actattatac atgaccgtgc aggtctcaat caattttacg
    23041 ctaaggttta tgcctttgtt ggtctgggaa tcggactatc tgctttggta tcaggcctta
    23101 tgttgacggt ctttcagtct cagttggttt acttgttgat gcaggggcgt ctctggttga
    23161 ccattgctac ttttgcggag ttggctttgg ttttcgttgc cagtagcatg gcttcaagaa
    23221 atagtccagc tgctcttcca gtatttttac tttactccgt tttaaacggc tttaccctta
    23281 gttttgtggt ggctttctat actccgggta cagttttatc tgcctttgta tcgagcgccc
    23341 ttctcttctt tgtcatggcg gcagttggta tctttaccaa gaaagacttg agtgggattg
    23401 gtcgagcaat gatggcagct ctcattggtt tactgattgc tatggtagtc aatattttct
    23461 tggctagtgg cttctttgat tatatgatta gcgtagccat ggttttggtt ttttcagggt
    23521 tgattgcttg ggataaccaa aagattcgcc tcgcctatga acaatcacaa ggtcgtgtag
    23581 cgacaggctg ggtggtgtca atggctctca gcatctatct tgattttatc aatcttttcc
    23641 taagtattct acgtatcttt ggtcgcaatg actaaaaggt ctatgaaatc agtgttttga
    23701 gtgacactga ttttttttaa attttctctt ttcttttctt ggaaataggt gtataatacg
    23761 tttattaaat ttttgaggag ttgtctatga agaaaagttt tatccatcaa caagaagaaa
    23821 tttcctttgt caaaaacact tttacccagt atttgaaaga taagctagaa gttgtcgaag
    23881 ttcaaggtcc tatcttgagt aaggtcggtg acggaatgca ggacaacctg tctggtgtgg
    23941 aaaatccagt atcggtcaag gttctccaaa tccctgatgc tacttatgaa gtggtgcact
    24001 cacttgctaa atggaaacgc cacaccttgg ctcgttttgg ctttggtgaa ggagagggtc
    24061 tctttgtcca catgaaagcc cttcgtccag atgaggattc cttggatgca acccactctg
    24121 tttatgttga ccagtgggac tgggagaagg ttatcccaaa tggtaagcgt aacatcgttt
    24181 atctaaaaga aacagttgag aagatttata aggctattcg cctgactgag ctagctgttg
    24241 aagcccgcta tgacatcgag tctatcttgc caaaacaaat tacctttatc catacagaag
    24301 aattggtaga acgctaccca gacttgacac cgaaagaacg tgaaaatgcg atttgtaaag
    24361 aatttggagc cgtctttttg attggtatcg gtggcgagtt gccagatggt aaaccgcacg
    24421 atggacgtgc accagactat gatgactgga caagcgagtc tgagaatggc tacaagggtc
    24481 taaatggtga tattcttgtc tggaatgagt ctttaggtgg agcctttgag ttgtcttcta
    24541 tgggaattcg tgtagatgaa gaaactctta gacgtcaggt ggaaattaca ggtgatgaag
    24601 accgcttgga attggaatgg cacaagtctt tgttgaatgg tctattccca ttgacaatcg
    24661 gtggaggaat tggacaatct cgtatggcca tgttcctact tcgcaagaga cacatcggag
    24721 aagtgcaaac aagtgtttgg cctcaagaag tccgcgatac ttacgaaaat attttgtaga
    24781 gaatcgaacc gcaaggttcg gttttctttc tctttttgtc tataatttgg tataataaac
    24841 agtatgaaaa tcgtatcagg aatctatggg ggacgtcccc tcaagacact agaaggcaag
    24901 acgacaagac ctacttcgga taaggttagg ggagccattt ttaacatgat tggtccctac
    24961 tttgaagtgg gacgagtctt ggacctttat gcaggtagtg gtggtttatc tatcgaagca
    25021 gtatcgcgtg gcatgtccag tgctgttttg gtggagcgag accgtaaggc tcagaccatc
    25081 gtggctgaaa atatccagat gaccaaggaa gttggaaaat ttcaactcct caagatggat
    25141 gcagaaaggg cattggaaca ggtatctggg gaatttgacc tcgttttctt agaccctccc
    25201 tatgccaagg aacaaatcgt agcagatatt gaaaaaatgg ctgagagaga gcttttttct
    25261 gaagatgtta tggttgtgtg cgagacggat aaagccgttg aacttccaga agaaattgcc
    25321 tgtctgggta tctggaagga aaagatttat ggaattagta aggtgacagt ctatgtcaga
    25381 taagattggc ttattcacag gctcatttga tccgatgaca aatgggcatc tggatatcat
    25441 tgaacgggcg agcagacttt ttgataagct ttatgtgggt atttttttta atccccacaa
    25501 acaaggattt ctccctcttg aaaatcgtaa acgggggtta gaaaaggctg tgaaacattt
    25561 gggaaatgtt aaagtcgtgt cttctcatga taaattggtg gtcgatgtcg caaaaagact
    25621 gggggctact tgcctagtgc gaggtttgag aaatgcgtcg gatttgcaat atgaagccag
    25681 ttttgattac tacaatcatc agctgtcttc tgatatagag actatttatt tacatagtcg
    25741 acctgaacat ctctatatca gttcatcagg cgttagagag cttttgaagt ttggtcagga
    25801 tattgcctgc tatgttcccg agagtatttt ggaggaaata agaaatgaaa aaaaagatta
    25861 gatggccctt atacgtcatt gcggccttga ttgtgacttt cttggcattt gtagtgccct
    25921 tgccttatta tatagaggtt ccaggtggtt cggaagatat tcgccaagtc cttaaagtaa
    25981 atgacacaga agataaggaa gctggtgcct atcaattcgt tacggttggt gttcaacatg
    26041 ccactttagc tcatatgatt tatgcttggt tgacaccttt tacagatatt cgtagtgctc
    26101 aggagactac aggtggttct tccgatgttg aatttatgcg aatcaatcaa ttctacatgc
    26161 aaacatcgca aaatatggcc aagtatcaag gactaaaaac agctggtaag gatatcgaac
    26221 tcaagtactt tggagtttat gttttgaatg tgacggataa ttcaaccttt aaagggattc
    26281 tcaatatctc tgatacagtc acagcagtca atgatcagac ctttgatagt tccaaagact
    26341 tgattgatta cgtcagttct caaaaattag gggattccgt caaggtcacc tatgaagagg
    26401 atgggcaaac caagtctgca gaaggaaaaa tcatcacctt ggaaaatggc aaaaatggaa
    26461 ttggaatcgg cttgattgac cgtacagagg taatcagcaa tgtcccaatt agcttttcaa
    26521 cagctggtat tggcggtcca agtgctggtc tcatgtttag tctagctatc tatactcaaa
    26581 tagctcaccc agatcttcgt aatggtcgta ttgttgccgg tacaggtacc attgaccgcg
    26641 atggtaatgt gggagacatt ggaggtattg ataagaaggt tgtagcttcg gctagggcag
    26701 gtgctgctat tttctttgct cctgataacc ctgttagcga agaagaacaa aaggcgcatc
    26761 cggacgcgaa aaacaactac caaacagccc tagaagcggc taaaacaatc aagacggata
    26821 tgaaaatcgt gcccgttaaa accctacaag atgcgattga ttacttgaaa aacaatccct
    26881 aatttttgag tgaagtttcc aaactagcgc acaaacagag aagatggtat aatagtcaaa
    26941 tggttcaatt attattcact ctaagtagtc atatgttctt tatttatgtg agtttttacc
    27001 ttttaaagga tcttgttaga tgggagaagg ttttaaaagt gacagatgat aatacaagaa
    27061 aagttcgttt attagtagcc ttttttagca ttgtcatagg ctacatcctg agttctttct
    27121 ttattagcct gtatcatttg tggcaagaag cgcttagagg attattatga aatcaagagt
    27181 aaaggaaacg agtatggata aaattgtggt tcaaggtggc gataatcgtc tggtaggaag
    27241 cgtgacgatc gagggagcaa aaaatgcagt cttacccttg ttggcagcga ctattctagc
    27301 aagtgaagga aagaccgtct tgcagaatgt tccgattttg tcggatgtct ttattatgaa
    27361 tcaggtagtt ggtggtttga atgccaaggt tgactttgat gaggaagctc atcttgtcaa
    27421 ggtggatgct actggcgaca tcactgagga agccccttac aagtatgtca gcaagatgcg
    27481 cgcctccatc gttgtattag ggccaatcct tgcccgtgtg ggtcatgcca aggtatccat
    27541 gccaggtggt tgtacgattg gtagccgtcc tattgatctt catttgaaag gtctggaagc
    27601 tatgggggtt aagattagtc agacagctgg ttacatcgaa gccaaggcag aacgcttgca
    27661 tggtgctcat atctatatgg actttccaag tgttggtgca acgcagaact tgatgatggc
    27721 agcgactctg gctgatgggg tgacagtgat tgagaatgct gcgcgtgagc ctgagattgt
    27781 tgacttagcc attctcctta atgaaatggg agccaaggtc aaaggtgctg gtacagagac
    27841 tataaccatt actggtgttg agaaacttca tggtacgact cacaatgtag tccaagaccg
    27901 tatcgaagca ggaaccttta tggtagctgc tgccatgact ggtggtgatg tcttgattcg
    27961 agacgctgtc tgggagcaca accgtccctt gattgccaag ttacttgaaa tgggtgttga
    28021 agtaattgaa gaagacgaag gaattcgtgt tcgttctcaa ctagaaaatc taaaagctgt
    28081 tcatgtgaaa accttgcccc acccaggatt tccaacagat atgcaggctc aatttacagc
    28141 cttgatgaca gttgcaaaag gcgaatcaac catggtggag acagttttcg aaaatcgttt
    28201 ccaacaccta gaagagatgc gccgcatggg cttgcattct gagattatcc gtgatacagc
    28261 tcgtattgtt ggtggacagc ctttgcaggg agcagaagtt ctttcaactg accttcgtgc
    28321 cagtgcggcc ttgattttga caggtttggt agcacaggga gaaactgtgg tcggtaaatt
    28381 ggttcacttg gatagaggtt actacggttt ccatgagaag ttggcgcagc taggtgctaa
    28441 gattcagcgg attgaggcaa gtgatgaaga tgaataagaa atcaagctac gtagtcaagc
    28501 gtttactttt agtcatcata gtactgattt taggtactct ggctctagga atcggtttaa
    28561 tggtaggtta tggaatcttg ggcaagggtc aagatccatg ggctatcctg tctccagcaa
    28621 aatggcagga attgattcat aaatttacag gaaattaggc tggagaacca gcctttttct
    28681 aaagataagg agaaatatga acaaaaaaac aagacagaca ctaatcggac tgctagtgtt
    28741 attgcttttg tctacaggga gctattatat caagcagatg ccgtcggcac ctaatagtcc
    28801 caaaaccaat cttagtcaga aaaaacaagc gtctgaagct cctagtcaag cattggcaga
    28861 gagtgtctta acagacgcag tcaagagtca aataaagggg agtctggagt ggaatggctc
    28921 aggtgctttt atcgtcaatg gtaataaaac aaatctagat gccaaggttt caagtaagcc
    28981 ctacgctgac aataaaacaa agacagtggg caaggaaact gttccaaccg tagctaatgc
    29041 cctcttgtct aaggccactc gtcagtacaa gaatcgtaaa gaaactggga atggttcaac
    29101 ttcttggact cctccaggtt ggcatcaggt caagaatcta aagggctctt atacccatgc
    29161 agtcgataga ggtcatttgt taggctatgc cttaatcggt ggtttggatg gttttgatgc
    29221 ctcaacaagc aatcctaaaa acattgctgt tcagacagcc tgggcaaatc aggcacaagc
    29281 cgagtattcg actggtcaaa actactatga aagcaaggtg cgtaaagcct tggaccaaaa
    29341 caagcgtgtc cgttaccgtg taacccttta ctacgcttca aacgaggatt tagttccctc
    29401 agcttcacag attgaagcca agtcttcgga tggagaattg gaattcaatg ttctagttcc
    29461 caatgttcaa aagggacttc aactggatta ccgaactgga gaagtaactg taactcagta
    29521 aaagataagc ctaaactcct atgtcactta tggatgtagg agttcttttt actagtttaa
    29581 gcaggactag aacaggtact aagaaaaaat agcaacttct aaaactaact tccagttttg
    29641 ggagagagat ggaagttact ttgagaagtt acggtaagaa aaaataatat gtattggatt
    29701 aaattttgtg atataataaa ggataagata ctattttagg agaagaacta tggaagaccc
    29761 aagtagtcag aatttgttgc tacaatttgt tttattgttt atcttgacgg tattaaatgc
    29821 ctttttctca gccacagaaa tggccatggt ttctcttaat cgtgcccgag ttgaacaaaa
    29881 ggcggaagaa ggagacagac gctacatccg cctgctgaag gtactagaaa atcctaacca
    29941 ctttttatca accatccagg taggaatcac cctgattaca atcttatcag gggcgagttt
    30001 aactgatact ctaggacgtg tgattgcctc ttggcttggg aatggcgaaa cagcacaagc
    30061 cgtggcaact tttctatcct tggcattttt gacctatatt tccatcgttt ttggggaatt
    30121 atatcctaag agaatcgctc ttaatctaaa ggatgccttg gcaattcgta cagcgccgat
    30181 tatcattggg atcggaaaac tagtcagtcc ttttgtttgg cttttagctg cttcgaccaa
    30241 tttcttgagc catttgactc ctatgtcatt cgatgatgcg gatgaaaaga tgacccgtga
    30301 tgaaattgcc tatatgctga caaatagtga agaaacattg gatgctgatg agattgagat
    30361 gctacaaggt gtcttttcgc tcgatgaact gatggcacga gaggttatgg ttcctcgaac
    30421 ggatgccttt atggtggata ttcaggatga tagtcaagcc attatccaaa gtattttaaa
    30481 acaaaattat tctcgtatcc cggtttatga tggggataag gacaatgtaa ttggaatcat
    30541 tcacaccaag agtctcctta aggcaggctt tgtggacggt tttgacaata ttgtttggaa
    30601 gagaatttta caagatccac tttttgtacc tgaaactatt tttgtggatg acttgctaaa
    30661 agaactgcga aatacccaaa gacaaatggc aatcttgctg gatgaatacg gtggtatggc
    30721 tggtttggtg actttggaag acctcttaga ggaaattgtt ggtgaaattg atgatgaaac
    30781 ggacaaagca gcaatagatg ttcatcagat tggcgaggat acctacattg ttcaaggaac
    30841 tatgaccctc aatgatttca ataattactt tgatgttgaa ctggaaagtg atgacgttga
    30901 tactatcgct ggttattatt tgacaggagt gggaactatt ccaacgactg agaaactcag
    30961 ctatgaattg gtcagccaaa acaaacagtt tatcttaacc aatgataaag tgaagaatgg
    31021 acgtgttacc aaggtaaaag tccaaatcac agaagtcgaa atagaagaag aaacagaata
    31081 aactagaggc ttttgtcacc aggcgtgacg aagctttttt caattgattt caaaagggaa
    31141 ttatgccatg gaaagaactc tgccacaaac tcgctccaaa agtttttaaa gttatacgta
    31201 tttattcgag ggaaaataaa aaaagccctt caaattgggc tttctgttca tttgaaacct
    31261 gataaatcaa gttatagaag gcggtagacg gatttgaacc gacgatcaag cttttgcaga
    31321 gccgtgcctt accacttggc tataccgcct taacgtttat tattatacct tgaaaatgat
    31381 tttcagtcaa tagttttttc agttttttgt accatagcgt gattcgaatg tgatgccaag
    31441 ttttctatgg gaaaaaattt accaaaaacc tactgaaacc ccttgaaaaa tctagctaaa
    31501 tagggtataa tatgggtaat catttgtcgt aggttttgtc tgaaatattg tccagacaag
    31561 gctcacagca gttaaatctt ctgaaaaagt cagatttagc tgctcttttt gtgctttttt
    31621 tcaagatttt gagcatttgt aacagaggct taaagattct gaaaattcgt caagaggaca
    31681 cggtgataag gggtttacaa ccatatgacg attagaaaag cctgattgac aaggcttgga
    31741 acttatttac aaaggagaat catcttggca ggacatgacg ttcaatacgg gaaacatcgt
    31801 acccgtcgta gtttttcaag aatcaaagaa gttcttgact taccaaattt gattgaaatt
    31861 caaactgact cattcaaagc tttcctagac cacggtctta aggaagtgtt tgaagatgta
    31921 ttgccaattt caaacttcac agacacaatg gagttggaat ttgttggata tgaaatcaag
    31981 gaaccaaaat atacgctaga agaagctcgt atccacgatg ctagctactc agcaccaatt
    32041 tttgtaacct tccgtttgat caataaagaa acaagcgaaa tcaagaccca agaagttttc
    32101 tttggtgatt tcccaatcat gacagaaatg ggtactttca tcatcaatgg tggtgaacgt
    32161 attatcgttt ctcagttggt ccgctcacca ggtgtttact ttaacgacaa agtagacaaa
    32221 aatggtaagg tgggctatgg ttcaactgtt atccctaacc gtggagcttg gttggaactt
    32281 gaaagcgact caaaagatat cacctacact cgtatcgacc gtactcgtaa gattccattt
    32341 acaaccttgg ttcgtgctct tggtttctca ggtgatgatg aaatctttga tatctttggt
    32401 gacagcgaat tggttcgcaa cactgttgaa aaagatatcc acaagaatcc aatggactct
    32461 cgtacagacg aagccttgaa agaaatttac gaacgccttc gtccaggtga gcctaagacg
    32521 gctgaaagct cacgtagctt gcttgtagct cgcttctttg acccacgtcg ttatgacttg
    32581 gcagcagttg gtcgttacaa aatcaataaa aaactcaatg ttaaaacacg tttgctcaac
    32641 caaaccattg cagagccatt ggtagaccct gaaactggag aaatcttggt agaagctggt
    32701 acgattatga ctcgtagcgt gattgaaagc attgaaagcc atttggatgg cgacttgaac
    32761 aagattgtct acatcccaaa cgatgcagcc gttgtgactg agcctgttgt tcttcaaaaa
    32821 ttcaaggtta ttgctccaac tgatccagat cgcgtcgtaa cgatcattgg taatgctaac
    32881 ccagatgaca aggttcgtac ggtgactcct gcagatatcc ttgctgagat gagctacttc
    32941 ctcaacttgg ctgaaggact tggccgtgta gatgatatcg accaccttgg aaatcgtcgt
    33001 atccgtgcgg ttggtgaatt gcttgccaac caagtacgtt tgggactttc tcgtatggaa
    33061 cgtaatgtcc gtgaacgtat gtctgttcag gacaatgaag tcttgacacc acaacaaatt
    33121 atcaatatcc gtcctgtaac agctgcagtt aaagaattct ttggttcatc acagttgtca
    33181 cagttcatgg accaacacaa cccgctttct gagttgtctc acaaacgccg tttgtcagcc
    33241 ttaggacctg gtggtttgac tcgtgaccgt gctggatatg aagtacgtga cgtgcactac
    33301 actcactatg gtcgtatgtg tccaatcgag acacctgaag gacctaacat cggtttgatc
    33361 aataacttgt catcttatgg acacttgaac aaatatggtt ttgttcaaac accataccgt
    33421 aaggttgacc gtgaaacagg tgttgtcacg aacgaaattg tttggttgac agctgatgaa
    33481 gaagatgaat atactgtagc tcaggctaac tctcgtctga atgaagatgg aacctttgct
    33541 gagaagattg tcatgggacg tcaccaaggg gtcaaccaag agtatccagc taatattgtt
    33601 gactacatgg acgtttcacc aaaacaggta gttgccgttg cgacagcatg tattcctttc
    33661 ttggaaaacg atgactccaa ccgtgccctc atgggagcca atatgcaacg tcaggctgtg
    33721 ccattgatta atcctcaggc accttacgtt ggtactggta tggaatacca agcagcccac
    33781 gattctggtg cggctgtgat tgctcagtat gatggtaaag ttacttacgc agatgctgac
    33841 aaggtagaag ttcgtcgtga agatggttca ttggatgttt accacatcca aaaattccgt
    33901 cgttcaaact caggtactgc ttacaaccaa cgcactctcg taaaagttgg tgatgtcgtt
    33961 gaaaaaggcg atttcatcgc tgacggacct tctatggaaa atggagaaat ggcgcttgga
    34021 caaaacccaa tcgttgccta catgacttgg gaaggttaca acttcgagga tgccgttatc
    34081 atgagcgaac gcttggtgaa ggacgatgtc tacacatctg ttcaccttga agaatacgaa
    34141 tcagaaacgc gcgatacaaa gcttgggcct gaagaaatca ctcgcgaaat tccaaacgtt
    34201 ggtgaagatg ccctcaaaga ccttgacgaa atggggatta tccgtattgg tgctgaggtt
    34261 aaagaaggtg atattcttgt aggtaaagta acacctaagg gtgagaaaga tctttcagct
    34321 gaagaacgtc tcttgcacgc tatctttgga gacaagtctc gtgaagtgcg tgatacttct
    34381 cttcgtgtac cacacggtgc cgatggtgtc gttcgtgatg ttaagatctt tacacgtgta
    34441 aatggagatg agttgcaatc aggtgttaac atgttggttc gtgtttacat cgctcaaaaa
    34501 cgtaagatta aggtcggaga taaaatggcc ggacgtcacg gaaacaaagg ggttgtctct
    34561 cgtatcgttc ctgtagaaga catgccttac cttccagacg gaactccagt cgacatcatg
    34621 ttgaacccac ttggggtacc atcacgtatg aatatcggtc aggttatgga gcttcacctt
    34681 ggtatggcag ctcgtactct tggtattcac atcgctacac cagtctttga tggagcaagt
    34741 tctgaagatc tttggtcaac tgttaaagaa gcaggtatgg atagcgatgc caaaacgatc
    34801 ctttacgatg gacgtactgg tgaaccgttt gataaccgtg tttctgtcgg agtcatgtac
    34861 atgatcaaac tccaccacat ggttgatgat aaattgcacg cgcgttcagt cggaccatac
    34921 tcaactgtta cccaacaacc actcggaggt aaggctcagt ttggtggaca acgtttcggt
    34981 gagatggagg tttgggctct tgaagcctac ggtgcgtcaa atgtccttca agaaatcttg
    35041 acttacaagt cggacgatat caacggacgt ttgaaagcct atgaagctat tacaaaaggc
    35101 aaaccaattc caaaaccagg tgttccagaa tccttccgag ttcttgtcaa agaattgcaa
    35161 tctcttggtc ttgacatgcg tgtcctagac gaagatgacc aagaagtgga acttcgcgac
    35221 ttggatgaag gaatggacga agatgtcatc cacgtagatg accttgaaaa agcccgcgaa
    35281 aaagcagccc aagaggctaa agcagccttt gaagctgaag aagctgagaa agcaacaaaa
    35341 gcggaagcaa cagaagaagc tgctgaacaa gaataagcag ttcacttaga atagaaaggg
    35401 aagaaatagt ggttgatgta aatcgtttta aaagtatgca aatcacccta gcttctccaa
    35461 gtaaagtccg ttcatggtct tatggagaag tcaaaaaacc tgaaacaatc aattatcgta
    35521 ccttgaaacc agaacgtgaa ggactctttg atgaagtgat ctttggtcct acaaaagact
    35581 gggaatgtgc ttgtggtaag tacaaacgca ttcgttacag aggaattgtt tgtgaccgct
    35641 gtggggttga agtaacgcgt acgaaagttc gtcgtgagcg tatgggacat atcgaattga
    35701 aagctcctgt atctcacatc tggtacttca aggggattcc aagccgtatg ggcttgaccc
    35761 ttgatatgag ccctcgtgcc ctcgaggaag ttatctactt tgcggcttat gtggtgattg
    35821 atcctaagga tacaccactt gagcacaagt ctatcatgac agagcgcgaa taccgagagc
    35881 gcttgcgtga atatggttat ggttcatttg ttgccaagat gggtgcggaa gccatccaag
    35941 accttttgaa gcaagtagat cttgaaaaag aaattgctga actcaaagaa gaattgaaaa
    36001 cagctactgg acaaaagcgt gtcaaagcca tccgtcgttt ggatgttttg gatgcctttt
    36061 acaagtctgg aaacaaacct gaatggatga ttcttaacat ccttccggtt atcccaccag
    36121 atcttcgtcc aatgttgcag ttggatggtg gccgttttgc ctcatctgac ttgaatgacc
    36181 tttaccgccg tgttatcaac cgtaacaacc gtttggctcg tttgcttgag ttaaatgcac
    36241 caggtatcat cgttcaaaat gagaagcgta tgcttcaaga agcagttgac gctttgattg
    36301 acaatggtcg tcgtggtcgt ccaatcacag gaccaggtag ccgtccattg aaatcattga
    36361 gccacatgct taaaggtaaa caaggacgct tccgtcaaaa cttgctcggt aaacgtgttg
    36421 acttctcagg acgttcagtt atcgccgttg gtccaactct taagatgtac caatgtggtg
    36481 tgccgcgtga aatggcgatt gaactcttta aaccatttgt catgcgtgaa atcgttgccc
    36541 gtgatatcgt gcaaaacgtc aaagcagcta aacgcttggt ggaacgcgga gatgagcgta
    36601 tctgggatat ccttgaagaa gtgattaaag aacacccagt gcttttgaac cgcgcaccga
    36661 cccttcaccg tttgggtatc caagccttcg agccagtctt gattgatggt aaggctcttc
    36721 gcttgcaccc acttgtctgt gaagcctaca atgctgactt tgacggggac caaatggcca
    36781 tccacgtacc actttcagaa gaagcacaag cagaagctcg tatcctcatg ctagctgctg
    36841 agcacatctt gaacccgaaa gatgggaaac cggtagttac tccatctcag gacatggttt
    36901 tgggtaacta ctacttgacc atggaagaag ctggtcgcga aggtgaagga atggtcttca
    36961 aagaccgtga cgaagcggtt atggcttacc gcaatggtta tgttcacctc cactcacgtg
    37021 ttggtatcgc aacagacagc ctcaacaagc cttggacaga agagcaaaga cataaggtct
    37081 tgcttacaac agttggtaaa attctcttca acgatatcat gccagagggg ctaccatact
    37141 tgcaagaacc aaacaatgcc aacttgacag aaggtgttcc agctaaatac ttcttgccac
    37201 ttggtggaga tatcaaggaa gctatcagca atcttgagct caaccctcca ttcaagaaga
    37261 aaaaccttgg aaatatcatc gctgaaatct tcaaacgttt ccgtacgaca gaaacttctg
    37321 ccctacttga ccgcatgaag aacctcggtt accaccactc aactcttgca ggattgacag
    37381 tgggtattgc cgatatccca gtcgttgatg acaaggctga aatcattgaa gaatcacaca
    37441 aacgtgtcga acaaatcacc aaacaattcc gtcgtggtat gatcacagac gacgagcgtt
    37501 acaatgctgt tacagctgaa tggcgtgctg cccgtgaaaa acttgagaaa cgcttgattg
    37561 ctaaccaaga tcctaagaac ccaatcgtta tgatgatgga ctctggagcc cgtggtaaca
    37621 tctcaaactt ctcacagctt gccggtatgc gtggtctgat ggctgctccg aatggacgta
    37681 tcatggaatt gccaatcctt tcaaacttcc gcgaaggttt gtcggtattg gaaatgttct
    37741 tctcaactca cggtgctcgt aaaggtatga ccgatacggc ccttaagaca gccgactcag
    37801 gttacttgac tcgtcgtttg gtcgacgttg cccaagacgt tatcatccgt gaggacgact
    37861 gtggaactga ccgtggtctc ttgatccgtt ctatcgcaga aggaaaagag atgatcgagt
    37921 ctctcgaaga gcgtctcaac ggtcgttaca ctaagaaaac tgttaaacat ccagaaactg
    37981 gtgcagtgat tattggtcca aatgaattga ttacagaaga caaggcgcgt gaaattgtca
    38041 atgctggtgt ggaagaagtg actatccgtt ctgtatttac atgtaacact cgtcacggtg
    38101 tctgccgtca ctgttacggt atcaacttgg cgactggtga tgcggttgaa gttggtgaag
    38161 cagttggtac aatcgctgcc caatctatcg gggaacctgg tacacagctt acaatgcgta
    38221 ccttccacac aggtggggtt gcctcaaata ccgatatcac tcagggtctt cctcgtgtcc
    38281 aagaaatctt tgaagcccgc aatcctaaag gggaagcggt tattacagag gttaaaggac
    38341 aagttactgc tatcgaagaa gatgcatcaa ctcgtaccaa gaaagtcttt gttaagggtg
    38401 aaactggcga aggtgaatat gtcgttccat ttacagctcg tatgcgtgtc gaagttgggg
    38461 gccaagtagc gcgtggtgct gctctgacag aaggttctat ccaaccaaaa cgtctccttg
    38521 cagttcgtga tgtcttgtca gttgaaacgt accttctcgg tgaagtacaa aaagtttacc
    38581 gtagccaagg ggtagaaatc ggtgacaaac acatcgaggt aatggttcgt caaatgatcc
    38641 gtaaagtccg tgtcatggat ccaggtgata cagatcttct catgggtacc ctcatggata
    38701 tcaatgactt tacagatgcc aacaaagatg tccttatcgc aggtggagtt ccagcgacag
    38761 gtcgcccagt ccttatggga attaccaaag cctcacttga aacaaacagt ttcttgtcag
    38821 cggcttcctt ccaggaaaca actcgtgtcc ttactgacgc agctatccgt ggtaagaaag
    38881 accatctcct tggacttaaa gaaaatgtta tcatcggtaa gatcatccca gctggtactg
    38941 gtatggcccg ttaccgtaac cttgaaccac atgctgtcaa cgaagaagaa taccttaatc
    39001 ctccagtaga ggaagaagga aatgaagaaa caacagaagt agttgtggat actgccgttg
    39061 aaactgtgga agaaacagta gaataagaga gaatgagaga acccgagggt tctctcattc
    39121 tctcttctga aaactttctc catttttcca cgaaatgtgg taaaataaaa gaaagaagct
    39181 gacaaaggag acgggtatgg aacaaacatt ctttatcatc aaaccagatg gtgtaaaaag
    39241 agggctagtg ggtgaagtgt taaagcgcat cgaacaacgt ggatttacaa tcgaaaaatt
    39301 ggagtttcgt tcacaggttt cagaagagtt gattgaccag cactatcagg acttggttgg
    39361 tcagagtttt tacccaccga ttcgtgaatt catgacttca ggtccagttc ttgtgggtgt
    39421 catttctggt cccaaagtaa tcgaaacttg gcggaccatg atgggtgcaa ctcgtccaga
    39481 agaagcttta ccaggcacta ttcgaggtga ttttgcaaaa gctgctggag aaaatgagat
    39541 tatccaaaat gttgtacatg gttcagattc agaagagtca gctaagcgag aaattgctct
    39601 ttggttttaa gagtggattg gctcaatcaa ttggttaaaa gctcatttga atagaaagta
    39661 tagtcaatta gtttaagaca tgacgcatga tatcaaactt tttagttttt gatatggtgc
    39721 gttttttgat taagtgacta ttagtttgtc tcgctccgtt aggtgcgaga caaaaaaaac
    39781 acccgctatg cgggtgtgcg tcgaaggtta taccaaaaaa actccaaacg cgatacaata
    39841 aaggtgttca atccaattgt aaagcgaaag gagaaaaata tggcacaaaa ggttctttta
    39901 tcctgttctt gtttcaattg actatatttc tatacttttt cttcctttct ctgtgaaaat
    39961 cgcaagtttt tcccaccttt tctcaatact gaaacaacat ctttccagaa cttcaataat
    40021 ttctttttgt gttaaatcat tatttaacta aatctttgga ggcaatacgt tttcaaagct
    40081 acttttttac cataggtttt gagccttcaa cacagattac taagccaaaa atgctctaaa
    40141 tttttgaaat tctaacaaat tccactcaac tttatcatca tctttgactt tcacaatatc
    40201 ttcctcaaaa taacatggaa taaagatagt tttcttttaa aacggttgta ggaggaaaat
    40261 ctctcctaca ctattattga ttctgagaga aaattgcaaa atatcatcta ccaaactttc
    40321 aattattaag attttttgca gtttatctga attagaatcg caaggcttct caataatata
    40381 attgtcgaat tttttcattc taacttcctc tccattattt cttcctttat caagaaaaag
    40441 ttacctcaat attaataaag tacaattcta aattaagtac aattctaaat taactccaat
    40501 tcttgattca tgataatatt tcagtaactc atcgtgcaat taaaaacaag gatttttatt
    40561 tcagatgatc catcgtgaac acttcatcag ccatctccca aattgccgga ttatgtgttg
    40621 cgataatgat tagcctatta tcatctcgaa gagatagcaa aatctccata atcaactgag
    40681 aggttgctgg gtctattgaa gctgttggct catctgccag aataaagggt ggattcttta
    40741 agataatttt tgccaaggca acccgttgcg attctccgcc cgataactca aagatgcgct
    40801 tatctaggtc aagataaacc aggccgacct gttctaaagc ctgcttctgc ctcaaccgct
    40861 gttccgaccg actcaacttt tgaccaatga gacctagctt aaggttttct tcaatacttt
    40921 ggttttcaat taagccaaag ttctggaaga ggtagcccaa ttcgtgacgg aaaaaatcac
    40981 ttgatttata attggccaag tctttacctc ggtaaaaaat cgtcccatca taaggttcta
    41041 atttcccaat catgttcatc aaggttgttt ttccgctacc acttgaacca attaaggcat
    41101 agacttttcc agcctcaaat gtcattgaaa gattcgaaaa taactctcgt tctccaaaag
    41161 atttactcac ctgtttaagt tcaatcatat taacctccct tcaaaacaag catggacatc
    41221 ttgttttctt tctgcatttg gacatgtaac tgtaaaagag atagaccagt aaagagtaac
    41281 aagactaaga aagcaactcc tatctctacc tgaagaaata cactcgcaac aaatcccagt
    41341 aaaaacacac ccagttgagc aaagagataa gtgcgatgga tttctaagaa cctgagacct
    41401 gcaatgcgtt tgataaaaat ggcacgtcta aattcttcaa agtagagcct attcatagtg
    41461 ttaaacaaca agattgaagt tgcaatccca agcacagctc ctgctagcat tacccaacgt
    41521 tccctctgga tattatccaa taatgtgatg tagttgtggt aacctgtttg catttctgag
    41581 acccaatttt caatgccttg tctctggata agctcctggg catcagacaa ttgattaaag
    41641 agaacgtagt tctgtactgc gtctatccaa aacaaaatgg actgtggacc agttgattgg
    41701 ggtgttataa caatgatgat tggatctttc aaaaactgct ggtaagaaat aggggtcgta
    41761 ttatacacaa aacgatcctg acctgattct aaataaccta ccgtagcagt catttgctgt
    41821 cgttcatctt gactagatat gcgactggtt aagtcgtctt caaaaacaga tttataatgt
    41881 tcttcctctg aacggaggtg ttcaggcagc aataagacaa actcccctac atcaaggtga
    41941 ttcatctttt gactcatttt agttgaactg taggacttac tttttcagag ggctccaaat
    42001 tttattttca tttacttttt ttagcccata aaatatttac catacttata aaaagtagta
    42061 atactccaac taaactgact tgtaatgaat tatatgtatt ttctgaagtg ttaggtaaat
    42121 aattttgaga gtacccttct cgggcaatag taataatatt gtgtgtgttt atttgtttaa
    42181 ttccaatagt agtttgaatt ctctcttcca actcaattaa attaacatta tctatgaaac
    42241 tatcccaact ttgttcattt ttatattcta attgtaacat attcaaatca tatctcaata
    42301 cgtttggtat atttaacact tttataccat tttttactgg taatgtttct tccagaattt
    42361 ttttacaaac atataattat agggctgatc ttttaatttg tatttatcaa taatcattgc
    42421 aatcgcacca gaaactttag gagcagcaaa tgagtttcca taaagatacg tatatcctcc
    42481 tagtgttgcc gaaaaaatcc aatcttcttt atatttttct gcattaataa agttattaag
    42541 tcctaattct gaaagagata atgttgagcc tgcaggcgca tatattgcat cagaatcccc
    42601 tttattgcta aaatctgata aattacctaa gcgatctatg cctccgaccg aaattgtaga
    42661 tgagaaataa cttggaacat caactactaa tcctggtttt cttacttttt tgcgtgaact
    42721 aataagtttc aataaatctg actgatttga tacatttagg gagtcattcc ctaatgcagc
    42781 tactataatt actccttttt gattcgcgta atcaatagcc tttttatact tcaaaaatgt
    42841 ttcaaaatca tttgttccgt cctcatattc tccatcaatc attaaatatt gtccagtact
    42901 aagattgata atatcattgc catcatctac agcatcaaaa attgctttta caatccaatc
    42961 tggactagct gacgacttac caaatattct atagacgtta atattaacgt gaggatttac
    43021 tccattaata ttgtcatccc ctacaatttg agctacaaca gccgtaccat gacctaatct
    43081 atcttgaata tctgatatta ttccttcctc tccctcctct tttcctaaat atcctttatt
    43141 ggggacatag ttttttgagt gatttgaaag atttttcagt aatccagtat tttgtaaatc
    43201 gactcctgaa tctaaaatag caattgaaac tttagaatta cttttagaat ataatttata
    43261 actttcgcct ccaccagtaa ctttatctat atcccattca tattttctta caatcgacga
    43321 ttctaagtta tctttgtcat
//
LOCUS       DAVKNL010000014        42903 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVKNL010000014 DAVKNL010000000
VERSION     DAVKNL010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 42903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42903
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.014"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(20..1978)
                     /gene="ftsH"
                     /locus_tag="VW411_000690"
     CDS             complement(20..1978)
                     /gene="ftsH"
                     /locus_tag="VW411_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU9793467.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEETGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
     gene            complement(1994..2536)
                     /gene="hpt"
                     /locus_tag="VW411_000691"
     CDS             complement(1994..2536)
                     /gene="hpt"
                     /locus_tag="VW411_000691"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEU9793468.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            complement(2540..3817)
                     /gene="tilS"
                     /locus_tag="VW411_000692"
     CDS             complement(2540..3817)
                     /gene="tilS"
                     /locus_tag="VW411_000692"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEU9793469.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETIFMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIKNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            complement(3814..5082)
                     /locus_tag="VW411_000693"
     CDS             complement(3814..5082)
                     /locus_tag="VW411_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEU9793470.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            complement(5202..5570)
                     /locus_tag="VW411_000694"
     CDS             complement(5202..5570)
                     /locus_tag="VW411_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEU9793471.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            complement(5563..5829)
                     /locus_tag="VW411_000695"
     CDS             complement(5563..5829)
                     /locus_tag="VW411_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEU9793472.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            complement(5887..9396)
                     /gene="mfd"
                     /locus_tag="VW411_000696"
     CDS             complement(5887..9396)
                     /gene="mfd"
                     /locus_tag="VW411_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEU9793473.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERDLAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTNICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNIDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKESKEENSI"
     gene            complement(9397..9966)
                     /locus_tag="VW411_000697"
     CDS             complement(9397..9966)
                     /locus_tag="VW411_000697"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEU9793474.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            complement(10036..11151)
                     /gene="ychF"
                     /locus_tag="VW411_000698"
     CDS             complement(10036..11151)
                     /gene="ychF"
                     /locus_tag="VW411_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEU9793475.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELNDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            complement(11235..11429)
                     /locus_tag="VW411_000699"
     CDS             complement(11235..11429)
                     /locus_tag="VW411_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEU9793476.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            complement(11494..12630)
                     /gene="dnaN"
                     /locus_tag="VW411_000700"
     CDS             complement(11494..12630)
                     /gene="dnaN"
                     /locus_tag="VW411_000700"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU9793477.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            complement(12789..14150)
                     /gene="dnaA"
                     /locus_tag="VW411_000701"
     CDS             complement(12789..14150)
                     /gene="dnaA"
                     /locus_tag="VW411_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEU9793478.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYNYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
     gene            complement(14363..15121)
                     /locus_tag="VW411_000702"
     CDS             complement(14363..15121)
                     /locus_tag="VW411_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU9793479.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            complement(15179..16360)
                     /locus_tag="VW411_000703"
     CDS             complement(15179..16360)
                     /locus_tag="VW411_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU9793480.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVIVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            16543..17022
                     /gene="rlmH"
                     /locus_tag="VW411_000704"
     CDS             16543..17022
                     /gene="rlmH"
                     /locus_tag="VW411_000704"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEU9793481.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            17066..17139
                     /locus_tag="VW411_000705"
     tRNA            17066..17139
                     /locus_tag="VW411_000705"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17100..17102,aa:Arg,seq:tct)
     gene            17304..17429
                     /gene="comC"
                     /locus_tag="VW411_000706"
     CDS             17304..17429
                     /gene="comC"
                     /locus_tag="VW411_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEU9793482.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRISRIILDFLFLRKK"
     gene            17450..18775
                     /locus_tag="VW411_000707"
     CDS             17450..18775
                     /locus_tag="VW411_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9793483.1"
                     /translation="MDLLGFGTVIVHFLIISHSYRLICKGRINRKELYVFGAYTLLTE
                     IVLEFSFYLLYLDKIGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVIFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDKDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            18772..19524
                     /locus_tag="VW411_000708"
     CDS             18772..19524
                     /locus_tag="VW411_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9793484.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            19576..19647
                     /locus_tag="VW411_000709"
     tRNA            19576..19647
                     /locus_tag="VW411_000709"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19609..19611,aa:Glu,seq:ttc)
     gene            19653..19726
                     /locus_tag="VW411_000710"
     tRNA            19653..19726
                     /locus_tag="VW411_000710"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19687..19689,aa:Asn,seq:gtt)
     gene            complement(19767..20309)
                     /locus_tag="VW411_000711"
     CDS             complement(19767..20309)
                     /locus_tag="VW411_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEU9793485.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            20488..21255
                     /locus_tag="VW411_000712"
     CDS             20488..21255
                     /locus_tag="VW411_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEU9793486.1"
                     /translation="MIGISLIPDLYNIIFLSSMWDPYGQLSDLPVAVVNNDKEASYNG
                     NTMAIGKDMVSNLKENKTLDFHFVDEEEGKKGLEDGDYYMVVTLPSDLSEKTTTLSNI
                     QSTAAYQSLTSEQQTEISDSVSQNSTDSIQSAQSIVALVQDLQGSLENLQNQSSNLST
                     LKNQSNQVSPITSTSLIGLSSGLTEIQGDVTSKLVPASQSIASGVNAYTTGVDKVSQG
                     ASQLSEKNATLTGSLDKLVSGSNTLTQKSSRLTAGVG"
     gene            21340..21615
                     /locus_tag="VW411_000713"
     CDS             21340..21615
                     /locus_tag="VW411_000713"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793487.1"
                     /translation="MADGSGKLAEGGTKLTSGLEDLQTGLASLGQGLGNASDQLKSVS
                     TESKNAEILSNPLNLSKTDNDQVPVNGIAIAPYMISVALFLQQYQQI"
     gene            21705..22019
                     /locus_tag="VW411_000714"
     CDS             21705..22019
                     /locus_tag="VW411_000714"
                     /inference="COORDINATES: protein motif:HMM:TIGR03062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEU9793488.1"
                     /translation="MAGILVYGGVQLIGLTANHEMRIFILIILTSLVFMSMVTTLATW
                     NSRIGAFFSLILLLLQLASSAGTYPLALTNDFFRSINPWLPMSYSVSGLRQTISINKS
                     FS"
     gene            complement(22120..24672)
                     /locus_tag="VW411_000715"
     CDS             complement(22120..24672)
                     /locus_tag="VW411_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEU9793489.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQSIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSNLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAIYLTTLLKFGLIGLSTFFSLNKLFQSIPQILKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(24734..26356)
                     /locus_tag="VW411_000716"
     CDS             complement(24734..26356)
                     /locus_tag="VW411_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9793490.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            26562..27587
                     /gene="trpS"
                     /locus_tag="VW411_000717"
     CDS             26562..27587
                     /gene="trpS"
                     /locus_tag="VW411_000717"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEU9793491.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            27739..29217
                     /gene="guaB"
                     /locus_tag="VW411_000718"
     CDS             27739..29217
                     /gene="guaB"
                     /locus_tag="VW411_000718"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEU9793492.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(29268..30365)
                     /gene="recF"
                     /locus_tag="VW411_000719"
     CDS             complement(29268..30365)
                     /gene="recF"
                     /locus_tag="VW411_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEU9793493.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(30368..30736)
                     /gene="yaaA"
                     /locus_tag="VW411_000720"
     CDS             complement(30368..30736)
                     /gene="yaaA"
                     /locus_tag="VW411_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEU9793494.1"
                     /translation="MEYKLFEEFITLQALFKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            30895..32145
                     /locus_tag="VW411_000721"
     CDS             30895..32145
                     /locus_tag="VW411_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU9793495.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYCSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            32142..33425
                     /locus_tag="VW411_000722"
     CDS             32142..33425
                     /locus_tag="VW411_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU9793496.1"
                     /translation="MTKVVFEEKYYPAVKEMVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGEIIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            33467..34288
                     /locus_tag="VW411_000723"
     CDS             33467..34288
                     /locus_tag="VW411_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9793497.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            34299..34844
                     /gene="pgsA"
                     /locus_tag="VW411_000724"
     CDS             34299..34844
                     /gene="pgsA"
                     /locus_tag="VW411_000724"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEU9793498.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            34841..35668
                     /locus_tag="VW411_000725"
     CDS             34841..35668
                     /locus_tag="VW411_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU9793499.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            35653..36492
                     /locus_tag="VW411_000726"
     CDS             35653..36492
                     /locus_tag="VW411_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU9793500.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            36485..37279
                     /locus_tag="VW411_000727"
     CDS             36485..37279
                     /locus_tag="VW411_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9793501.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            37338..38156
                     /gene="mreC"
                     /locus_tag="VW411_000728"
     CDS             37338..38156
                     /gene="mreC"
                     /locus_tag="VW411_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU9793502.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            38156..38650
                     /gene="mreD"
                     /locus_tag="VW411_000729"
     CDS             38156..38650
                     /gene="mreD"
                     /locus_tag="VW411_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEU9793503.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            38744..39922
                     /locus_tag="VW411_000730"
     CDS             38744..39922
                     /locus_tag="VW411_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEU9793504.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            40146..40925
                     /gene="rpsB"
                     /locus_tag="VW411_000731"
     CDS             40146..40925
                     /gene="rpsB"
                     /locus_tag="VW411_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU9793505.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAALETQADSIEEIVEVVEGDNA"
     gene            41004..42044
                     /locus_tag="VW411_000732"
     CDS             41004..42044
                     /locus_tag="VW411_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEU9793506.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            42290..42613
                     /gene="tnpB"
                     /locus_tag="VW411_000733"
     CDS             42290..42613
                     /gene="tnpB"
                     /locus_tag="VW411_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU9793507.1"
                     /translation="MIIQLSDLGQVHLVCGKTDMRQGIDSLAYVVKTHFELDPFSDQV
                     FLFCGGRKDRFKVLYWDGQGFWILYKRFENGKLTWSSTEKDVKALAPEQVDWLMKGFS
                     ITPQI"
     gene            complement(42754..>42903)
                     /locus_tag="VW411_000734"
     CDS             complement(42754..>42903)
                     /locus_tag="VW411_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEU9793508.1"
                     /translation="IEQVWKEIRKRGFKNKAFRTLEDVIQGLEKEVIKSIVNRRRTRM
                     LFENR"
BASE COUNT        13436 a         7408 c         7897 g        14162 t
ORIGIN      
        1 gctccagcct ctctcagggt tatttttcgt cattcatttt tgactttact tcatcatagg
       61 atagtgcatg agattcctct tctactgctt caggcatctt tcctgtttcg taaagagctt
      121 taatttgtgt actatccaat gtttcgtatt tcaataatgc ttctgcaatt aacttgtgag
      181 tttcacgatt tgactgaata atttcagcag ctttatttcg tgcctcattt aataatgaac
      241 gaacctcttc atcaatttca taagctgttt gttctgaaat tgatttttga ggactctgtg
      301 caccaagcat agcatggttt ccttcatatt gtactgggcc aagtttttca ctcataccgt
      361 actctgtaac cattgcacgt gccatttgtg tcgcttgttc aaagtcgttt gaagctcctg
      421 tggtttggac attaaagata atttcttcag ctacacgtcc acccattaag ccagccaatt
      481 gctctttcat atcttcttta gatagaagca tttgatcctc tttaggaagt gcaatcatgt
      541 atccgcctgc acggccgcgt ggtacaattg taaccttatg gacaacgcga gcattcgaca
      601 agactagacc aacaatggta tgtcctgcct catggtaagc aaccaattct cgttcttttt
      661 gtgaaactgt cttatctttc ttagaaggtc cagcaataac tctatcttct gcttcatcaa
      721 tatctgaagc atcaattatc gatttattgc gacgagcagc aactaaagct gcttcattca
      781 agacattctc taaatcagca ccaacaaagc ctggagtttg ttgagccact aatttcaaat
      841 caacatcttc tgctaaaggc ttattcttag cgtgaacttt caagattgct tcacgacctt
      901 taacatcagg acgaccaacc aatacttttc tatcaaaacg tcctggacgc aaaagggcag
      961 ggtcaagtac atctgaacgg tttgtcgcag cgatgacgat aatcccttca tttccctcaa
     1021 aaccatccat ctcaatcaaa agttggttca aggtttgttc acgttcgtca ttacctccgc
     1081 cgagaccgac tccacgttga cgtccaacag catcaatttc atcgataaag atgatagctg
     1141 gtgctgcttt tttggcatcc tcaaaaagag agcgaacacg actagctcca actccgacaa
     1201 acatttctac aaagtcagaa cctgagatac taaagaatgg aacacctgct tctccagcga
     1261 ctgccttagc aagcaaagtt ttacctgtcc ccggaggtcc ctccaaaaga acacctgctg
     1321 gaatacgggc tccaagtttt gtgaatcgtt ttggatcttt taagaactca acaacttcaa
     1381 ctagttcttg tttttcttcc tcagctccag caacatctga aaatcttact ttaatatctt
     1441 ctttatttgc tgctttagcc ttactacgtc caaaactcat tggattacgg ccattgcctc
     1501 ctcccatatt tcccatcata gagaatagga agaagaatag aattccaaat ggcacaatgg
     1561 atacgagtag attaatccat ataccacttg aactttcatg cttaacagtt acttctgctt
     1621 tatggtcagt agcaagtttt tgcaattctg atacggtagt atctgcagga agaatagtgc
     1681 tggtaaattt ctctacctta gtaacagatg gcgtgaaaaa ctgaatacct gtttcttctt
     1741 tacttgtttt aggattttta tagacaccag aaacttcgat aacactacca tttggttggt
     1801 aagttaattc ttttacatta ccatcggtaa tttcttgtac caactcagta tagttgattt
     1861 gctgacttcc tcctgagtta ttcccagaat agaaatactg gaatcctgtc acaaggaaaa
     1921 agataaataa taaccataga aaaggatttt taattaaacc attattttgt tttttcatta
     1981 aagattattc tttctaattt gaatacactt cctctttcaa tactccaata taaggaagat
     2041 tacgataatt ttctttgtag tctaaaccat aacctactac aaactcattt gggatagtaa
     2101 agcaagtata gtctgcctca atttctacaa cacgtccttc tggtttatcc aacaaggttg
     2161 caattttaac agaagctgct tctctttctt taaacatatc tcgcaaattc ttcaaagttt
     2221 gacctgtatc aatgatatct tctacaaata gaacatgtct tcctttgata tcttgagtca
     2281 catcttgttt aatattgata acaccactac ttgctgttcc accatggtag ctagaaacca
     2341 tcatgaagtc catttcaata tgtgtatcaa tatgtttgac caattcagcc ataaaaggaa
     2401 tagatccttt taaaatccca actaagattg gattttttcc tgcatagtct ttagttaatt
     2461 gagcacctag ttttttagct gcttctgtaa tttcatcgtg tgaaacgagg acttttttaa
     2521 tatcgttttc taacattttt tacctatcta ttttttctat ataaagtaca gtgttcatta
     2581 tatcattttt cgttttttta ctcaaattat tggtcgcaat tcccaaaatt gagacaattt
     2641 caccaaattg ctcaataata agagcagagt ttctcttttc catagggatt ttcaaatcaa
     2701 taaataaacg tctgagtttt tttctatgcc catttttaat caaaacatct cctgtttttc
     2761 gatgacgaat gtgtatggat gtttcacgtg aaacaggtat ttgttgaatt aattcacctt
     2821 ctaatggaag tccaaaagaa aataaatatc cttgataagc tacctgattt tgatagtgta
     2881 acacaagttc atcttccttt tcatcagcct gcggactgat tttacaaatc tgaaactgtt
     2941 ggtactcttt tatcaattca tagccatttt taatcggatg acgatactgg cttttagatt
     3001 ttaaaatctg ctgaacttca gcaaactgag cttttgtaag attcaaatct ggaaaacgat
     3061 tcagataagt ttgaagtaaa actctttgtg tagactcaga gtaagaaaat aactgctgta
     3121 aatcttccac attaatattg ttagataatt cagctattgc caaatcataa tctaaaattt
     3181 cattgccaat gcctaagatt gcatccctaa atcgaggatt ttctttttcc aattctggta
     3241 agtaagaatt tcgaatacga tttcgaaaat aatgattctc ctgatttgat gtatcttcaa
     3301 agtgaaaaat tgatggaaag tcttttttct gaaaatgcaa gaagggacga atgatttcta
     3361 tctctccgac tacttgcttc tccttaattc ctgatagata gcgcaagcga gttcctcgaa
     3421 tcaagcgcat aaaaatcgtt tccacctgat catcagcatg gtgggcagtg actaaagctg
     3481 tcgcacctgt ctttttcatg acctcttgaa aaaaatcata acgaaaattt cgtgcacgcg
     3541 cttctgaaaa ttctcctgaa aaattgctga tataaatagg aagctctgct tcagcagcca
     3601 acttccttaa ttccttttct tcccaatctg attcaattct ctgcttatga ttcacatgag
     3661 ctagaatcaa ttcaatctct aactcttttt gataagtaga caatacctta aatagaaaca
     3721 tggaatctaa tccaccagaa agagctagaa ccgccttagc atgctttttg aaatatccct
     3781 tcttgagaaa atgatttaaa aaatctggtt ccctcatttt agaacctcat aaacatcctt
     3841 ggctatctta gaaatcgtat cataatcaga attcttagtg aaaatagaaa gaataaatgg
     3901 agaatctgca tagacaacac ccgtatcatg cttaaattca tccgcatctc caattttatg
     3961 agctacttta acagaaacac ctttggcaat tcgctgacta tcaaaatctg ttttagtcaa
     4021 agactctagc acaaatccat tttgattata aatagcttcc ataaacttcc cggccatctt
     4081 agaagaaatc aatttttctt ttggatccca atcatctccc ataatggcag acatcttgga
     4141 tttgaatgtg gcatcagatt ggtttgaaat gtaatatccc aatagattat gagctacatt
     4201 atcagattct tttgatactt tcgtaattaa atcctttaaa gaatattctt tattatcttc
     4261 ttttttagga agactaccac ttccctctgg tttataagaa cctggaaaat cattgactgc
     4321 agatacgtat tttacagtcg tatctaactg ataaagaccc tcatttattt tttcttgcgt
     4381 ataatagaga taagagagtt tcaaaacgct ggctgcatac atcttttcat cttgattgat
     4441 accagcttct tttccagtag tcagttgctt aacataaata gagaaagaat ctttctgata
     4501 tttttcagat aacatttctt gaactttact catccgatta tcttcttcag aagttgattc
     4561 tttagctacc catccagcct gatcaatatg tagaaattct cttccttcta caaacatggt
     4621 cttgtcgatt gatacttgcg aataagctga taaggatgat ttcacttctt ttaaatcata
     4681 aggactattg tacagtttaa agtcagattc taaccatact ttttttattg ttggagttac
     4741 ctctgattga tcatataaaa atcgtttgtc cgcagctata aattgatgat ttgatagctt
     4801 aaatactgga attccttgtt tatttaagcg ccactcggtt atttgaaaac ttgttttggg
     4861 agtcaatttc ccagattcca ctactaaatc ttcattcgca taaacaggaa cctctccata
     4921 aaccatggga gaacttaatt tttctctaaa ataaatacca aagtcagatt gtgaaaggta
     4981 ataaatttct ttcgaagtat agacgacttc tttttctgtg ctaacgactt ttgaaatggt
     5041 caaaaaactt ggtagcaaca aaataattaa gaatttacgc attcttcctt tccttttctt
     5101 cttgtaaacg caataattga tttttatcag ccaattcttc cagcttttca tctgacaaac
     5161 tcaaaaattg ctctattacg tctaataaat tttccatttt atcacctttg aagcaagtca
     5221 ggaatcgtat aaactttttc cctcgactta gaataatagt acttcgctcg tgtatattta
     5281 gcagcataat cttcatcttt caacttggta gcaaatgctg tctccttatc cttttcatca
     5341 ctcaaagttt gatactgagt ttgcaagtct gctaattgct gacgtctttg gagtaattgc
     5401 tgataactct gcgctaaatt aaaagttggc aagataaata atagcataat caaaatcaat
     5461 acccctccca taaaacgatt ccgtttttgt cgttctttca tcaggtagcg acgacgttgg
     5521 tattcatttt gaataaaaga attattcaat tgtacaatat ttttagacat tttcttctac
     5581 ccgtgtttca ctgataattt catacattcc tgctgcatct tctttttttg tactatcttt
     5641 catctctagt acttttacaa gcagcaactt attgccaaag cgaatttcaa cttggtcatt
     5701 aactttcaag tccgttgaac ttttggccaa gattccatta accttgattc tacctttatc
     5761 tgctacttcc tttgcgactg tacgacgctt gataattcgc gatactttta aatatttatc
     5821 taatctcatc cttattacct caatttatta ttgtaccatt tttatctatt ttatagaaga
     5881 aaaatatcaa atggaatttt cttccttaga ctcttttatc tctaataaac tttctccaaa
     5941 aatcagcaaa ccttctaaaa tttcataatc tttcttattt tggacatcaa atacaagctc
     6001 cattaatccc ttattctcag cgatgcctgc ttttaagttc gttacggata aagctttaaa
     6061 ataatcttga gctaaaaaca gtcgttgagt gactttttca aattgaattg taattttatt
     6121 atcttttctt tccacacgtt gaacaaagac cttgtccaag tatgatttga ccaaaccaat
     6181 ctctaacaga taggctacta catctgggta ttctccaaaa cggtctatca actcctcttg
     6241 taactcttca taattgacac ggttgtcaat ttgacgaatt ttcttgtaaa tttcaatctt
     6301 atgtcgttga tcagaaatat aagtatcagg aagataggca tcaatttgca aaatcaactc
     6361 agcattccct ttggttcttg tgttagcgtt agcattaccg tttcgtttag caatagcttc
     6421 ctctaataac tgcgaataca attcaaaacc aacagaatca atgaaaccag actgggattt
     6481 tcctaaaaga tttcctgctc cacgaatcga aagatctcgc attgcaatct taaagccaga
     6541 gcccaattct gtaaatcctt taatcgcttc taatctcttt tcagagactt cactgattga
     6601 tttttctgga cgatacatga gataagcata agcaatacga ttactacgac cgactcttcc
     6661 tcttaactga tataaggttg acaagcccat atggtccgca ttttcaataa ataaagtatt
     6721 agcatttgga atgtccaccc ctgtctcaat aatagtagtc gtcaccaaga tatcgtattg
     6781 tccctcaata aagtctaata gagtattttc caactggact tcactcattc gaccatgaac
     6841 atatccaatc gaagcctccg gaatcaactc ctgtaattct gaaaccttct gaacaattgt
     6901 gtcaactttg ttgtaaagat aataaacttg acctccacgc tccatttcac gcaagacagc
     6961 atcacgaatg acactatcat tcttttccaa aacataggtc tgaacaggat agcgattagt
     7021 cggcggagtt tcaataacag ataaatctct gattcccagc atagacatat ggagggtacg
     7081 agggattggc gtagcggtca aggttaggac atccacttgt ttcttcagtt ctttcaaagt
     7141 ttccttatgc ttgacaccaa atcgctgttc ctcatcaata atcatcaagc ccaaatcagc
     7201 aaacacaaca tcttttgaca aaacacgatg tgttccaatc aaaatatcga cttgaccgtt
     7261 tttcaatttt tcaagtgttg cagtctgctc ttttttactt ctaaagcgac tcaacacatc
     7321 aatattaact gcaaaatttt ggaatcgttc cttaaaattc gtatagtgct gttgcgctaa
     7381 aaccgtcgtc ggaactagaa tgacaacctg tttgtgatca ttgactgctt taaaggctgc
     7441 acgcatagca acttcagtct ttccaaaacc aacatcccca actaaaagtc gatccattgg
     7501 ctgagaagcc tgcatatccc tcttgatttc ctcaatacta cgaagttgat catccgtttc
     7561 aacataaggg aaagcatcat caaaggcatc ttgatcatca tcatcagctg agaaagcaaa
     7621 acccttcaac tgactacgtt cagagtagag tttgattaaa tcatcagcta tatcctctac
     7681 ctggttctta accttttgct tggccttttt aaaatgaccg tcatttaatt tattgagttt
     7741 tggagcttta ccatcacttg aaatatattt ggacagtaga tgaatctgtt ccacggggat
     7801 agaaatttga tcaccatttt ggtattggac actgacataa tcgcgatgaa ttcccttgat
     7861 ttcaatggtt tcaattccta gatattgacc aatcccatgg atatgatgga caacatagtc
     7921 ccctttttca agttcattgt aatcttttaa tctctctgca tttgaaacat gttgtcttcg
     7981 aaaacgacgc tttaatttct tttgaaaaat ctcatgttca gttatcaata aaatcttttc
     8041 atctacaaaa tgaaaaccat gtctgagatt accctctatt aagtttacag attctttaca
     8101 gatatttgtc ttatctctag aatccaattt aatctgatat tcctctaaca tatcctccaa
     8161 tgttttactt cccattgaat tgctagactg cagaataatg gtgtaatcca tttttttata
     8221 tcgttcaatt tcttctttta gaaaagaaaa ctgattgaaa aattcctgca taggatattg
     8281 attgaattga taaattttgt caaatttgag atttcctaaa cccttttgaa gattagagaa
     8341 aaaggtcact ggactttgtt ttttatagat ttgttcaata tcagaaaaat actgcatatc
     8401 agaaaatgct ttactattct gtaattcttc tgtaaagtac tgcgctaagt ctctttcaaa
     8461 gacttcatac tgattcatca atttttgata atcatcaaag aatattggag tatctttttc
     8521 aatataatca aagacagtcc atgtcttatc atagcacaaa gataaaaact tccgagagtc
     8581 tgcatgactt tgtttttggt gaaaacttga aagaatttct tctaggtatg atttcaaaat
     8641 aggtgataaa gtttttgaaa tttgtttttc taaagctgac tgtcctcgtt gataatcctt
     8701 ttctctcaaa agcatatcac tagctggaaa gatagtgagt tctgtcttat tttctttcga
     8761 taattgtgtt tctacttcaa atgacctgat accatcaatt tcatcaccaa aaaactcaat
     8821 tcgacaaggt tctaactggg atatttcaaa aatatctaaa atatctcctc gaagactaaa
     8881 ttcgccctga gtttgtactt gagtaacttt tcgatagcca ttttccttta actgatggat
     8941 aaacgcgtgt tgatcatatt cttcaccaac tgagattttt acaatactat ctttgaatgc
     9001 attgggagac ggtaaaatca atcgacttgc tgcgatatta caaactaaaa tccctttctt
     9061 agatgaatca gtcaaaaaac gcaaggcttc aacccgtgaa ataatttttt cctgtgaaga
     9121 catcaaaaac tccaccatag gagcatcatc taccaaaaat ggatagacga gttcctcacc
     9181 caagatagaa ataagatcac taacaagtcc ttctgcttct ccataagttg acgtcaataa
     9241 cacaatccta tcttcttttt ctaaactgct tgcaattgca agagccttag tagatgttga
     9301 taaaccaagt attagttgtc ttttcttatc tgttaaattt tgatgccatt ttttaatctg
     9361 atcattttct gagaataaat ctaataaggt caccatttat ccgttatacc tctgcattgt
     9421 tttctcaaaa tttttctctt gtaaatagta gtttacagaa tcgtcaactt tgtcaacaga
     9481 ctgtaaaata ccgatataat catccctgtc aaacttactc aaaacatgat gaacaactga
     9541 cataccattt ttaggtcttc caattccaat cttaacacgg ttaaagacct gagttcctat
     9601 atgttgaata atagacttga taccattatg accacctgct gagccttttg ctcttaaacg
     9661 aattttccca acttccatgt caagatcatc gtaaatgata agtaaatcgt caatatccaa
     9721 accatagtaa gttaataaag catgaactgc ttttccactt tcattcataa aggtcgttgg
     9781 tttaaccaga taaatttttt ctccatttag gaaaaaggat gctaggtcag cttgaaatat
     9841 cttatcgtgt gtaaaagtga cattctgttt cttcgctagt tgatcaatca acataaaacc
     9901 aacattgtgt tttgtttcaa aatatttatc ccctggattt cccaagccta caagtaattt
     9961 ggtcatttat ttttcctttc aaaagccaaa agggttggaa ttttttccaa cctaattgac
    10021 accatttatt aatttttaga cattaaagcg gaattccatg atatcgccat cttgaacgat
    10081 atattctttt ccttcttcac gcaagcgtcc agcttctttt acggcctttt cagatccgta
    10141 tttcactaga tcttcatatg acatggttac tgcacgaata aagccttttt caaagtctga
    10201 gtggataata ccagctgctt gaggagcctt cataccacgt ttgaaagtcc aagcgcgaac
    10261 ttctttttca ccagctgtga agtaagttcc caatccaagc aagtggtaag ctgcacgcgt
    10321 caacttatct acacctgatt ctgtcaaacc aatggcttca agaaactctt ttttatcttc
    10381 atcattcaat tcagaaattt cttcctcagc acgcgcagaa ataacgacta cttcagcatt
    10441 ttctgtcgct gcaaattcac gaatttgttt gacatagtcg atagagtcag gttctgaaac
    10501 cacatcctcg tccacattag ctacataaag aactggttta gtcgtcaaaa ggaaaagacc
    10561 tttgacaacc ttttgttcct catctgtaaa ttcaatggta cgagctgatt tcccgtcttc
    10621 taggactggt ttaatctttt gaagaacatt gaattctgct actgattctt tatctttttg
    10681 cgtacgtgcc atcttttcta cacgcgcata tcgtttgttc actgattcta agtcagcaag
    10741 aatcaattcc agattaatgg tatcaatatc tgcaagtgga tctacaaagg cgtcttcacg
    10801 tccttgctcg cgcattacat tttcatcatc aaaagcacga actacgtgaa caatcgcatc
    10861 tacttcacga atattggcca agaatttatt ccctagcccc tctccttttg aagctccttt
    10921 tacaatccct gcaatatctg taaattcaaa tgttgtggga actgtctttt taggagttat
    10981 catttcagtt agtttttgta ggcgttcatc tggaacttcc accattccaa catttggatc
    11041 aatcgtcgca aatgggtagt ttgctgcctc tgctcctgct tttgtaattg cattaaatag
    11101 tgttgattta ccaacgtttg gcaaaccaac gatacctgct gtcaaagcca tatttctcat
    11161 tctccgtttt catttcaatc cctaatatta taacacaaaa agggataaag tgctaaccct
    11221 ctaactaagg gttctcagtc aataatttta ttcatttttc gctcaaaatc atatcgcccc
    11281 atcatgacaa catgctcaca attactacat tttattttga tatctgctcc tacacgtgta
    11341 atttcccaac gattagcctt tttaccagtc gacttgattg tacaagcgtg tgattttttc
    11401 atctcaacaa aatttccaac ttgatacata ctactctcct tttctttttc gattatatca
    11461 taaaagaggc gagccaggct caacctcttt cacttaattt gtacgaactg gtgtaatgag
    11521 ctgcatgaag tcttcgtcag tatctgctgg cacaagagta aatggacgaa cagctgagat
    11581 aaagctaata gtcacctttt cgctatttaa agctttaaga gaatcaatca agtaagttgg
    11641 gttgaaacta atggtcaaat cttcaccagt aacctgatca gtatcgattt cttcgtttac
    11701 tttaccaact tctggagagt gaacatgggc gctaacaacc ccatccttaa tttcaagttt
    11761 cacagtacca ttttgagtcg cacttgataa aagacgggca cgctccattg actggcgtaa
    11821 gtttaccaca ttaaaagtaa tagtagtgtt aaagtctgtt ggaatcaagc gatctgtatc
    11881 aggatagttt ccttctagga gacgagtata gaagctaata ttttcgcttc taaagaggat
    11941 ttggttattg gcaaagaaaa tctctacagt ttcgatatca tctgtaaata ccgctgaaaa
    12001 ttcgcgtaga gaacggctag gaattacgac atcaaaatca tcactatttt tttcaagagt
    12061 caattttttc tggcttaggc gatgagagtc tgttgcaact gtttttaact ctttgtgttg
    12121 actcaatacg aagtggacac ctgttaaaat cggacgactc tcttgtgtac ttgcagcaaa
    12181 ggctgtttca ttaataattt tcttgagtaa ttttgtttca agtattaaag gagtgcttgc
    12241 tgaaatttct tggattcgtg gatattgttc gctatctttt ccttttaggg taatttctga
    12301 tttgccactg gttaaaacaa tttgattttg ttcaatttct ttaaaatcaa gagttacatc
    12361 aggtaaacta gatactacat tgataaagaa agaagcttca agaaggatcg aacctaaaga
    12421 agtaattaac aaaccagcat cttcattttt ttgagaaata aaattttcaa ttgaaatttg
    12481 accatttgaa ccaattaaag taataccttc attggtcacg tcaattttta ctgttgataa
    12541 aataggaatg gcatttttag aactaatagc tctcttagta gtatttaatg cttgtagaaa
    12601 taaattttta ttaattgaaa aatgaatcat ggattctcct ttatttattt ttagtattag
    12661 aaagataata gtaataatag agtctgtgaa atctgtggaa aacagccttt aaacaagtca
    12721 tatcaagttt tttagcttgt ttaaaaaatg tggataaaaa agataaaaaa ctaaaagtta
    12781 tccacaaatt atttgatttt ctttttgatt gattcaattt ctaaacgtaa attatcgtct
    12841 tgatcaatca aagattttat tttggcatgg gcatgaatga ctgtggtatg atcttttccc
    12901 ccaaattcct tcccaatttt tggaagacta ttatctgtta gttctctaga taaatacatg
    12961 gctacttgac gggccaaaac aatattttga aggcgtctac ttcccttcat ttctttgata
    13021 ctaacaccat aaaagttacc aacttcagtt tggattttat caattgggat gacgagcatt
    13081 tggctaacat cttgtttgcg ggctctaatg gcttctgcag caatatcaat agtgatatcc
    13141 ttgatttttt ttactctggc aattaaagtg atgtcgttga tggctccctc aagatctcga
    13201 acatttgaat caaattgccc agctaggtat tctagagtat cactttggaa attgtagcct
    13261 aaatgttccg tcttactttg taaaatggca atacgtgttt caaagtcagg gggggtgata
    13321 gtttgtgtca atccccaact aaaacgcgtg acaagcctct cctcgagccc ttctagatgt
    13381 tttggactac gatcactcgt taggacaatc tgtttttgct tgtcatgaag ggcgttaaag
    13441 gtattgaaaa attcttcctg agttgcgact ttttttccgc tgagtgactg gatatcatcg
    13501 attaacaaaa gatcaagact acgataggtc tttttaaact tttccatttc cccaagtctt
    13561 aggtgatcaa gaaagtcatt aataaagctt tcggcaggga tatatttaac acgcgcatta
    13621 ggaatatttt ttagaatttc atttccaata gcgtttaata agtgagtctt accaaggcct
    13681 ggtcctccat agataaaaag agggttatag gtcagagcca aatcttcaga gacagctaaa
    13741 gcggctgata cagcccaaac atttccatcc ccttgaataa agttatcaaa ggtatacttt
    13801 tcttttaatc ccgtatctga ataaggaata gatactaact ttggactata gttataaaga
    13861 gttaaatttg tagcttcttc aacttgtgag ctagtcgtat cttgaggttt ggtgaaaata
    13921 tagtggggag ttatttcagc gtcataaatt tcaaaaccag ctactacaat aatatctttt
    13981 agttgttttt cccagaccat ttccatttca gagcgaggta gaaatatagt ggcaacattt
    14041 tcctctacct tgatgagttc agcttgaata gcatagaaat catacatgga tcgagtcagt
    14101 ctttcttgtg caaattctaa tatacgattc caaaattgtt tttctttcaa tcctttctcc
    14161 tcctttacta tttaaaagtt taatactaga gatattttac catagatagc aagaattttc
    14221 cacaagctgt gaaaaataat ccacaatgtt accaaacttt atccacaggt tggggataaa
    14281 agaagaaatt attgatttat taagcttttt atcgaaaaac atagtggata accttgtgag
    14341 ataaaaaaat aaaagaacag ccttatttca ggctgttgat aattctacta tattcttctt
    14401 gatttgaaaa agaaataatg atttttccac tgtctttttt agatagttta atttctacat
    14461 ctaatccgag tagttttctt aactgttttt cttcattttg tatgaaatga ttagtttttt
    14521 gctgtttctt ttgttttttc tctgtcagaa gagcttctaa tttccttaca gaaatatctt
    14581 cttctataat ccgttgaaag aaatagtctt gttgttcctt atttaaccca actagggaac
    14641 gcgcatgggc ttgtgatagt ttgccatttt ctacttctga aagaatctgt tctggcaagg
    14701 aaagtaaacg aatggagttg ctgatatatg gacgagactt gcccatctta tctgcaattt
    14761 cagcatgggt gaatcctttc tctacgagag attcataggc gcgtgcttct tctattgggt
    14821 ttaaattttc tctctgtaaa ttttcaataa tggactggac catcatctct tggtctgaaa
    14881 tctgtttaac aacagctggg atagaccgta gaccagctaa aagtgaagcc cgatagcgtc
    14941 tctctcctgc aaggatttca taaccaataa caggagattg acgaacaata atcggttgaa
    15001 tgaccccatt ttctttgata gactgtgcta gttcatctag tttttctcta tcaaattctt
    15061 ttcggggttg atagggattt ttttgtatat ctgtgataga aatcatttca aatttttcca
    15121 tgattctaca ctaacacatc ttttctctta tgtaaagctt tctttacata gatgtcaatt
    15181 aagattctaa atcacctgaa ctcttgttaa gtttgataga ggtagtttct tctttcccgt
    15241 tacgatagta ggttatctta atggtgtctc cgatagaatg gttgtaaaga gcactttgta
    15301 agtctgttga tgaagcaatc tctttgtcat ctacttttgt aattacatcg tatttttcaa
    15361 ggtgaccatt ggcaggcata ttactttgta ccgaacgaac aattacacca gatgtaacat
    15421 tacttggaat attgagtctt ctgatgtcgc ttgtactcac attagataaa ttaaccatct
    15481 ggattcccaa agctggacgc gtcacttttc cgtttttttc taactgttca ataatattga
    15541 tagcatcatt tgcaggaatt gcgaaaccaa gaccttctac agatgttcct ccatttgtag
    15601 caattttact tgaggtaatt ccgataacct gcccttgaat attgatcagt gggccgccag
    15661 agttacctgg gttaatagca gtatcagttt ggatggcttt tgtagaaata gcttgtccat
    15721 cttccgattt taaggataca tttctattga gactggatac gataccttga gtgacagtat
    15781 ttgcatattc agaacctaac gggctaccga tggcaatagc agtttctcct acagttaact
    15841 tactagaatc accaaactca gctactgttg tcactttttc tgaagagatt ttgacgacag
    15901 caatatcaga gaaagtgtca gctccgacaa tttctccagg tactttagtc ccatctgaca
    15961 atcgaatatc tactttgctg gcgccattaa taacgtgatt gttggtgacg atgtaagctt
    16021 ctttatcatt ctttttataa ataactccag atccttcact agagattcgc tgagaatctg
    16081 tgtcagtatc atcattgcca aatacgctat tttgtctgtt tgccgaataa gtaataacag
    16141 aaacaacagc atcttttact ttgttaacag cctgtgttgt tgaattttcg ttcttatagg
    16201 cagtttgtgt aatagtacta ttgttgttag agttgtttac actacttttt tgagttagtt
    16261 gagttattga aaaactaccc aaggctccac taaaaaagct aatgacgata acgactaata
    16321 attgaaacca ttttttgtaa aatgttttta gatgtttcat atttgcctcc atatgtttga
    16381 attactgaaa gtataaactg actagcttaa ttataactta aacacaaaag ttttacacaa
    16441 actgtggata actcttttga aattgtgatt ttcttaattg aaatctattt tttattttgt
    16501 gaataagatg tgaaaaaata gagaatatgt tagaatagag tcatgaaaat taaagttgta
    16561 acagttggga aactgaaaga aaagtattta aaagatggta tcgcagagta ttcaaaacga
    16621 atttctagat ttgctaagtt tgaaatgatt gagttatcag atgaaaaaac accagataag
    16681 gccagtgaat cagaaaatca aaagatttta gaaatagaag gtcagagaat tttatcaaaa
    16741 attgctgacc gtgatttcgt tattgtgtta gccattgaag ggaaaacttt cttctcagaa
    16801 gaatttagta agcagttaga agaaacttct ataaaaggat tttctactct tacttttatt
    16861 attgggggaa gtttaggatt atcatcatct gtaaaaaata gagccaatct ttctgtcagt
    16921 tttggtcgcc taaccttacc ccatcagtta atgagactag ttcttgttga acaaatctat
    16981 cgcgctttta cgattcagca gggattcccc taccataaat agaaaattga cttttaattg
    17041 aatttttggt agaataattg tattaggtct catagctcag ctggatagag cattcgcctt
    17101 ctaagcgaac ggtcgcaggt tcgaatcctg ctgggatcaa tataatagca aagctgggaa
    17161 ttttcccggc ttttttctta aaaaagtaca ctttgggaga aaaaaatgac agttgagaga
    17221 attttatcta aaacgaaatt ccattttgta taatggtttt tgtaagttag cttacaagaa
    17281 aaaacatttt aggagatttt attatgaaaa acacagttaa attggaacag tttgtagctt
    17341 tgaaggaaaa agacttacaa aagattaaag gtggggagat gaggatttca agaatcatcc
    17401 ttgattttct tttcctacga aaaaagtaat gaaataagga gaaagagtaa tggatttact
    17461 tggatttggg acagttattg ttcatttttt aattattagt cacagttacc gtttaatttg
    17521 taaaggtcga ataaatagaa aagaattata cgtttttggt gcttatacat tactaactga
    17581 aatagtactt gaattttcct tttatcttct atatttagat aaaataggga ttgaaagatt
    17641 tttatttcct ttgggcttat attcctattt tcgatggatg aaacagtatg agagggatag
    17701 aggactattc ctaagtttac tactatctct tttatatgag agcactcata actttctgtc
    17761 cgtaattttc tcctctataa caggagataa ttttgtttta caatatcatt tcccattctt
    17821 tttcgttgtg acggtgttaa cctattttgt tacattaaaa atcatttact atttccattt
    17881 ggaactagcc tattttgaca aggactacct ttatcctttc ttgaaaaaag tattttttgc
    17941 tttactattg ctacatattg tatctttcgt ttcagatatg gtaagtacga ttaaacattt
    18001 gaatagtttt ggaagtattt tgtcatctat tgtctttatc tctctccttt tgaccttctt
    18061 tgcaatgaat tctcataaag ttcaaatgga gaaagagatt gctttgaagc agaagaaatt
    18121 tgaacagaaa catttacaga attacacaga tgaaattgtt ggtctgtata atgaaatccg
    18181 tggttttcga catgattatg ctggaatgct tgtcagcatg cagatggcaa ttgacagtgg
    18241 taatttacag gaaattgaca gaatttacaa tgaagtttta gtcaaagcaa atcataaatt
    18301 gcgttcagat aagtacactt actttgattt gaacaacata gaagactcag ctttacgaag
    18361 tttggttgct cagtcaattg tctatgctcg aaataatggt gtagagttta cactggaagt
    18421 aaaagatacg attaccaagc ttccaattga actattggat ttggttcgta tcatgagcgt
    18481 tttattgaat aatgctgtcg aaggatcggc tgatagctat aaaaagcaga tggaagtagc
    18541 agttattaag atggaaactg aaacagttat tgtgattcag aattcatgta aaatgacgat
    18601 gactccttca ggagatctat ttgccttagg attctccact aagggaagaa atcgcggagt
    18661 cggattaaat aatgtgaaag aactactaga taagtacaac aatattattt tagaaacaga
    18721 gatggaaggc agtacattta gacaaatcat tagatttaag agggaatttg aatgaaagtt
    18781 ttaattttag aagatgttat tgaacatcaa gtgagactag agagaatatt ggatgaaatt
    18841 tcgaaagaat cgaatattcc aatatcatac aagacaacgg gaaaagtccg tgaatttgaa
    18901 gaatacattg aaaatgatga agtaaatcag ctttatttcc tagatatcga tattcatgga
    18961 attgagaaaa agggatttga agtggctcag ctcattcgtc attacaatcc ttacgctatt
    19021 atcgtcttta tcactagtcg atcagagttt gcgactctaa cctataaata ccaggtatca
    19081 gccctagatt ttgttgataa ggatatcaat gatgagatgt ttaagaagag aattgagcaa
    19141 aatatcttct acacgaagag tatgttactt gaaaatgaag atgttgtaga ttatttcgac
    19201 tacaattaca agggaaatga tttaaaaatt ccttaccatg atattttgta tattgaaaca
    19261 acaggggtat ctcataaatt gcgcattatt ggtaagaatt ttgcaaaaga gttttatggt
    19321 accatgacag atattcagga aaaggacaaa catactcagc gattttattc tcctcacaag
    19381 tcatttttgg taaatatagg taatatcaga gaaattgatc gaaaaaactt agaaattgtt
    19441 ttctatgaag accatcgttg tcctatttca agattaaaaa ttagaaaatt aaaagatatt
    19501 ttagagaaaa aatctcaaaa gtgattgaca attagcaaga aattgatata atggttatat
    19561 ctgctacaga tagaaggtcc gttggtcaag gggttaagac accgcctttt cacggcggta
    19621 acacgggttc gaatcccgta cggactattt tatccgccat agctcagttg gtagtagcgc
    19681 atgactgtta atcatgatgt cgtaggttcg agtcctactg gcggagtata gataaaaggg
    19741 aacacagctg tgttcctctt tttgtatcaa tttgtatcac caagcatttt cataaggaag
    19801 tctgttattt cttgagaact ttcttttttt ccatgtgcaa tccaagtttg gcagacacca
    19861 aaaagtgcat gagttagata gatgctacta tattctaatt cagtggtatt tagattcagt
    19921 tgcataaatc gcttttgtaa atctgtacta agcatgatat gaagtttatt tcgtaagaaa
    19981 ttttggattt ctttagtccc attttcagaa agaagggcag ccagaagtgg ttctgactct
    20041 agatattcaa aaacttctaa aatagcgtct cttttgtgat gagcatgttt ttgaaaaata
    20101 tattcaaatg tatggaatag cttgctttga tagtgctcaa tcatatcata cttatcctta
    20161 tagtgagtat agaagctgga acgactaatt ccggcttttt ctactaattt gacagtagaa
    20221 attttatcaa atggctgttc catcagtaat tgtaccatag cattttcaat agttcgcttt
    20281 gtttttaagc gtttgttact ttcttgcata tttcctcctt gtaaacaaat tagactatat
    20341 gtctaaaaat agatttttta tcttgtaatt tagatttttt aatgtataat ctattatatc
    20401 aaaattttag acaatatgtt taaaaaagga gaaactaagt ttaaagaatg gaaagcaatt
    20461 taaaaaaaac caacctttat tattgtcatg atcgggattt ctcttattcc agatctgtac
    20521 aatatcatat ttttgtcatc aatgtgggat ccatatgggc aattgtctga cttacctgtg
    20581 gcagttgtaa ataatgataa agaggcttcc tataatggta atactatggc aataggaaaa
    20641 gacatggtgt ccaatttaaa agaaaataaa accttggatt ttcattttgt agatgaagag
    20701 gaaggaaaga agggattgga agatggcgat tactatatgg tagtgacttt accaagtgat
    20761 ttatctgaaa aaacaactac attatccaat attcaatcga cagcagctta tcaatcattg
    20821 acaagtgagc aacaaactga gataagtgat tctgtatctc aaaattcaac tgatagtatt
    20881 caatcggctc agtcaattgt agctttagta caagatttac agggaagttt agaaaactta
    20941 caaaatcaat cttctaatct ttcgacttta aaaaatcaat ctaatcaagt atcacctatt
    21001 acttctactt ctttgatagg attgtcaagt ggattaacag agatacaagg agatgttact
    21061 agcaaattag ttcctgccag tcagtcgatt gcatcaggtg taaacgcata tactacaggt
    21121 gttgataaag tttctcaggg cgcaagtcaa ctaagtgaaa aaaatgccac cttgacaggt
    21181 agtttggata aactagtttc aggctcaaac accttgacac aaaaatcttc tagattgaca
    21241 gcaggagttg gttaattaca atcaggatct gggcaattag cagacaaatc cagtcagtta
    21301 ctttcaggtg cttctccatt agagaataga gctaataaat tggcagatgg atctgggaaa
    21361 ctagcagaag gtggaacaaa gttaacttct ggattggaag atttacagac aggacttgct
    21421 tctttaggac aaggactagg taatgctagt gatcaactca aatcagtatc aacagaatct
    21481 aaaaatgcag agattttgtc aaatccactc aatctttcaa aaacagacaa tgatcaagtt
    21541 cctgtaaatg gaatcgcaat agctccttat atgatatcag ttgctctttt tttgcagcaa
    21601 tatcaacaaa tatgatattt gcgaaattgc cttcaggacg tcatccagag agccgttggg
    21661 cttggttgaa atcttgagct gaaataaatg gtattatagc tgttttggca ggaattttgg
    21721 tatatggagg agttcagctt attggtttaa ctgctaatca tgagatgaga atatttattc
    21781 tcatcatcct aacaagttta gtattcatgt ctatggtgac cactttagca acgtggaata
    21841 gccgtatagg agcttttttc tcacttattt tgcttttact acagttagca tcaagtgcag
    21901 gtacttatcc acttgctttg acaaatgatt tctttagatc tattaatccc tggttaccaa
    21961 tgagctattc agtttcggga ttacgacaaa caatctctat caacaagtca ttttcctagc
    22021 tgtcatacta gttctattta ctagtttagg tatgctagcc tatcaacata agaaaatgga
    22081 agaagattaa aaaaatcgac cgattaactg gtcgattttt tatgccttag atgactttcg
    22141 tctgtgatta tagattccaa atagtaagag agaagtaaag gaacagattg ctccagtaat
    22201 aaaaccattg ggaatgaagg aaagtgtaat agttcctttc cccttgggaa tgtcaacttt
    22261 cataaatcca gtttgagctt gtttaatttc tattttctta ccatcttggt aggcagacca
    22321 acctttgtca taaggaatgg tgaagaaaat agatgtatct tgttggacat catatgtagc
    22381 aaaaaccttg tttttagaag ttgatactgt gacaggttgt tctttaattt tttgaattgc
    22441 ctcggtgaaa gttttggtat ctaaacgata gaaggtagga gattcaaatg atacttgtga
    22501 atttccaggg aaactaacat tgatattgaa agtttttttc tctttagtat atcctagatt
    22561 aaagaaggag aagacattat cagttgtaaa agtctttttt tcaccattta caaggatgtc
    22621 aaccttcttt tgtttatcgt tagaaaagtg aaggtttatg aaagagagat aaacttggct
    22681 gttttctgga acttcaattt gatactggat tgctgcatct tcatttgaag aacttgtgac
    22741 actaatcaaa tcattagtat tttctatttt ttctgttttt tcataaggta ttggagaaaa
    22801 ataatcaaaa ttgacgttag caagttgatt taaaaatgag gcctgattat ccaaggtatg
    22861 ttcattgaac ttgacatcat tgtaaacaga ttgactcgca actgcaatcg gaagagagta
    22921 ttgattttca tatagggtaa gattatcttt ttgatagata tctttaaagc catacttatc
    22981 aataggactg tctgagatat tgtactggat accaaataaa ctatcagcca aaatactatt
    23041 atttgcatat cggagattga gattagtccc agaggattta aaaccaagtt tatctaaagt
    23101 agagcttgat gaacgatttc gaacagatga aaattgagag attccattgt agttgaattt
    23161 catactgtca tttcctgtct gagtttgtag tttttcagta cgagtaaatt gatttccaat
    23221 atatgttgag aaagattcca tagctgggat atctcgacta taagcacttc gagaagcaaa
    23281 tccccattcc ttagcaattc cgtccatttg agatgaagca tttaaactca tttcaaccag
    23341 tataaataaa gagattagaa tggcaaatag attcacagat ataaactttt tgataactgc
    23401 aaggagtaaa agagaataga caaccaaaaa ttcaagagta agcagaatat tcaaatctgt
    23461 taaaaaagaa taatgcgatt ttagatagat ggtagctaaa aatcctgcta ctacaagaaa
    23521 aagcgaaact aaaaaattcc agactttaag ttctttcaga cgctttaaga cttctgctgc
    23581 tgtgtaaatt aacaaggtag agaaaatcca agcatagcga tgtaaaaaca tgtttggagt
    23641 atgcatgcct tgccaaaata agtcaagagc ttctatgtaa aagcttgcaa ttagaaatgc
    23701 aaagaatatt acatatatga gtttcacgtg aaacttaata gatttcagcg taaaaaataa
    23761 aatggtcaaa ataaagggaa atagtccaac aaaaatcatt gggatggccc catactttgt
    23821 tgtgtcaaag gaaccaatga attgcttagc aaagagatca agataccagc tactttcagt
    23881 ttgaaacttt gtaacttcag tcaatttttc cccatgtgtc tgtaaatcaa atagagtggg
    23941 aagagtcata atcaaactag ccataccagc taaaaaggag ataactatga aatcaagaac
    24001 agatgatttt cgagtcttaa agtcccacga aatttgacag agataccaga aaataagaaa
    24061 caatactgtc atatatccaa aataataatt ttgaataaat aagattgaca gacttgtaaa
    24121 gtacaatagg agtttctttt cagttatcag tagatgtaaa ccagttataa ttaaaggaat
    24181 caagataaaa acatctagcc aggtttttat ctctaattga ctgacagtga aactcatcag
    24241 agcataggaa gtagataagg ctagttttaa aatctgaggg atagattgaa acaatttatt
    24301 caaactaaaa aaggttgaca gaccaatcaa tccaaatttt aagagagttg tcagatagat
    24361 agcatctggc atattcgtta gatcaaaaaa gtaaaccaga ggcgcgagaa aactacccaa
    24421 gtaataacta gatagggcat agaagtttag ccctagacca cttgtaaagg tgtaaaacag
    24481 attactattt ccatgtagga tatttcgtaa ggctacatca aaaataacgt attgatgaaa
    24541 gccatctcct aatagaggag agttgtcgct attccagtag atactttgag atagatatac
    24601 tccagacata atcactacag gaatgatgaa agaaataaaa taggttcgat atgtttttaa
    24661 aaatgatttc atgttacctc gtagaatgat agaaaactca gttggttaac ccaactgagt
    24721 tttgaagttt tatttagtct ttccaaagtt ctttaacttt tgcttgtact tctgcatttt
    24781 ctaggaattc atcgtaggtt tcatcgatac ggtcaatgac gccattttta gataagacaa
    24841 tgatatggtt agccaaagtt tgaataaatt cgtggtcatg gctggcaaag atgattgatt
    24901 ctttaaagtt tttcaatcca tcattcaagc ttgagataga ttccaagtcc aagtgatttg
    24961 ttggatcatc aagtacaagg acatttgatt ttaagagcat gagttttgaa agcatgacac
    25021 gaactttttc tccccctgac aagacattta caggtttgtt aacttcatct ccagagaaga
    25081 gcatacggcc gaggaagcca cgtaggaaag tattgtcatc ttcttcttta cttgcgaatt
    25141 gacgcaacca gtcaagaatt gattctcctc ctgcaaaatc agctgagtta tcttttggta
    25201 ggtaagattg actagttgta actccccact tgacagttcc ttcatagtca atatctccca
    25261 tgattgcacg aattaatgca gtcgtttgaa tatcattttg tccaataagt gctgtcttat
    25321 catctggacg caagatgaaa ctaatattat ccaagatagt ttcaccatca atctttacag
    25381 ttaaattttc tactgtcaag agatcattac caatctcacg ttccgcttta aagttgataa
    25441 atggatattt acgactagat ggcacaatct cttctagctc aatcttatca agcattttct
    25501 tacgtgatgt tgcctgcctt gacttagaag cattggcaga gaaacgagca acaaattctt
    25561 gcaattgttt aattttttct tctgctttag cattacggtc tgctagcaat ttagcagcaa
    25621 gctcagaaga ttccttccag aagtcgtagt ttccgacata gagtttgatt tttccaaagt
    25681 caaggtcggc catgtgagta caaactttgt ttaagaagtg acggtcgtgg gatactacga
    25741 taactgtgtt atcaaagtca atcaagaagt cttctaacca agtaatcgat tggatatcca
    25801 aaccgttagt aggctcgtcc aagagaagaa catctggttt accaaaaagt gctttggcga
    25861 ggagaacctt tactttttca ccgttggcca attcgctcat gttttggtag tgtaattctt
    25921 ctggaatgtt taggttttga agtagttgag aggcttcact ctctgcttcc caacctccaa
    25981 gttcggcaaa ctctccttcg agttcggcag cacgaacccc gtcctcgtct gagaaatctt
    26041 ccttcatgta gatagcatct ttctctttca tgatgctata aagtttttca tttcccatga
    26101 taacgacatc aatggcacgt tcatcttcgt agtcaaagtg attttgacga agaacagaga
    26161 gacgttcatc tggaccaaga gagatgtgac cagtagtagg ttcgatatct ccagctaaaa
    26221 tttttaaaaa ggttgatttt ccggcaccat tagcaccgat taatccgtaa gtatttcctt
    26281 ctgtaaattt gatattgaca tcatcaaaaa gtttgcgatc actaaaacgt agtgaaacat
    26341 cagatactgt aagcaatgtt tttctcctat atgtgtaata tatttattct actagaaaat
    26401 acagaaatat tcaaattttt atttgtcaat tttgtgtaaa ttatatttac agtatccttt
    26461 acacaaatct gtaaaaagca aggctgattt attttgataa attacggtta tttcattaaa
    26521 aaaatgctat aattgaaagg actatatcga aggagaacaa aatgactaaa cccattattt
    26581 taacaggaga ccgtccaaca ggaaaattgc atattggaca ttatgttgga agtctcaaaa
    26641 atcgagtatt attacaggaa gaggataagt atgatatgtt tgtgttcttg gctgaccaac
    26701 aagccttgac agatcatgcc aaagatcctc aaaccattgt agagtctatc ggaaatgtgg
    26761 ctttggatta tcttgcagtt ggattggatc caaataagtc aactattttt attcaaagcc
    26821 agattccaga gttggctgag ttgtctatgt attatatgaa tctagtttcg ttagcacgtt
    26881 tggagcgaaa tccaacagtc aagacagaga tttctcagaa aggatttgga gaaagcattc
    26941 cgacaggatt cttggtctat ccaatcgctc aagcagctga tatcacagct ttcaaggcta
    27001 attatgttcc tgttgggaca gatcagaaac caatgattga gcaaactcgt gaaattgttc
    27061 gttcttttaa caatgcatat aactgtgatg tcttggtaga gccggaaggt atttatccag
    27121 aaaatgagag agcagggcgt ttgcctggtt tagatggaaa tgctaaaatg tctaaatcac
    27181 taaataatgg tatttattta gctgatgatg cggatacttt gcgtaaaaaa gtaatgagta
    27241 tgtatacaga tccagatcat atccgcgttg aggatccagg taagattgag ggaaatatgg
    27301 ttttccatta tctagatgtt tttggtcgtc cagaagatgc tcaagaaatt gctgatatga
    27361 aagaacgtta tcaacgaggt ggtcttggtg atgtgaagac caagcgttat ctacttgaaa
    27421 tattagaacg tgaactgggt cctattcgtg agcgccgtat tgaatttgct aaggatatgg
    27481 gagaagttta taatatgatt caaaaaggta gtgaaagagc gcgtgaagtt gcgggtcaaa
    27541 ccctatctga ggtaaaaggg gcaatgggac ttcattactt taactaagtt tattttacaa
    27601 gaaactatct tttctatctc gaagaaaaga tagtttttta tttacccctg taacatttga
    27661 caaattttta tagaatggta tgatagatac aatataaaaa gagtcaagct caaaaagaaa
    27721 gaaaagagga aacttcgaat gtctaattgg gacactaaat ttttgaaaaa aggttttacc
    27781 tttgatgatg tattgcttat tccagctgaa agtcatgtgt tgcctaacga tgcagattta
    27841 acaactaaat tggcagataa tttgacttta aatatcccaa ttattaccgc tgccatggac
    27901 acagttacag agagtcaaat ggccattgct attgctcgtg caggcggtct cggagttatc
    27961 cataaaaaca tgtcaattgc tcaacaagca gacgaggttc gtaaggtaaa acgttctgaa
    28021 aatggagtta ttattgatcc gttcttcttg acgcctgaac atacaattgc tgaagcagat
    28081 gagcttatgg gtcgttaccg catcagtggt gttccagttg ttgaaacact tgaaaatcgt
    28141 aaattggttg gtattttgac aaaccgagat cttcgtttta tttcagatta taatcaacca
    28201 atttcaaacc atatgactag tgaaaatctt gttactgctc ctgtgggtac ggatcttgca
    28261 acggctgaga gtattcttca agagcatcgt attgaaaaac ttccgttggt cgatgaagaa
    28321 ggcagtcttt ctggtttgat cactatcaaa gatattgaaa aagttattga gtttccaaat
    28381 gcggctaaag atgagtttgg tcgtcttcta gttgcaggtg cagtaggtgt tacttcagat
    28441 acatttgaac gtgcagaggc tctttttgag gcaggagcgg atgcgattgt tattgatact
    28501 gcacatggtc attctgcagg tgtcttgcgt aaaattgccg agattcgtgc tcatttccca
    28561 gatcggactt tgattgctgg aaatattgct actgctgaag gtgcacgtgc cctttatgaa
    28621 gcgggtgtag acgttgttaa ggttggtatt ggaccaggtt ctatctgtac tactcgtgtg
    28681 attgctggtg ttggtgttcc gcaagtaaca gctatctacg atgctgcagc tgttgcgcgc
    28741 gaatatggta aaacgattat tgctgacggt gggatcaagt attctggaga tattgtaaaa
    28801 gcacttgctg caggtggaaa tgctgttatg cttggatcta tgtttgctgg aactgatgaa
    28861 gctccaggcg aaactgaaat cttccaagga cgtaaattca agacttaccg tggtatggga
    28921 tcaattgctg ctatgaagaa aggttcaagc gaccgttatt tccaaggttc tgtcaatgaa
    28981 gcaaacaagc ttgttccaga aggaattgaa ggtcgtgttg cttataaagg agcggcagct
    29041 gatattgttt tccaaatgat tggtggtatt cgctctggta tgggttactg tggtgcagct
    29101 aaccttaaag aactacacga taatgctcaa tttattgaaa tgtctggtgc tggtttgaaa
    29161 gaaagccatc ctcatgatgt gcaaattact aatgaggcac caaattattc tatgtaaaaa
    29221 acaatgaaaa gaactccagt gaaaacagga gttcttttac aatgttgtca atttccattt
    29281 acagcagctt taccatcctg aatagtgaag atacttagat tttctggcag attttgaaga
    29341 tggtctaagc ttgttgttgt gataaaggtt tggattgatt gagaaatcgt ttctaataat
    29401 tttaactgtc tagtgttgtc aagttcactc atcacatcgt caagcagtaa tataggagat
    29461 tctgtggtaa tgctttccat taattcgatt tctgctaatt ttatcgagag gacgagacta
    29521 cgatgttgac cttggcttcc gaaactagca tccatcccat ttatataaaa agaaatgtca
    29581 tctcgatgag gaccgacacc agtattcttt ttaaataaat ctctggatct acttttttct
    29641 aaagcaattt tgaaagattc ggataagttt tgtttgtcag ttatattgac agaagattga
    29701 taggatattg acaactcttc gatctgatta gagagttcaa aatgtttctt acgcccaaat
    29761 gattctagtt tttttatgaa atctaagcgg tgattcatta cacgacatcc ataatcaact
    29821 agctgatcat ctaacacaga aaggaatgtt tcatctattt tttgagctga ttttaggtaa
    29881 gtgtttcttt gctttaggat gtggttataa ttggttaagt cagataaata gattggctta
    29941 atttgcccaa gttccatatc aatgaatttt cgtcgaatcg aaggtgctcc tttaattagt
    30001 tgtaaatctt caggagcaaa taagacaaca ttcatgtgtc ctacataatc tgaaaggcgt
    30061 gcctgtttta agtgattaac ttttgttaca cgaccttttt gtgttagttc gatttctagg
    30121 ggaatggatc cagttttttt ctgaacgaga cctgaaagat gaagttgttc ctcatcaaaa
    30181 tgaatgagat ttttatctgt tcgagttcga tgactacgcg ttaaggctaa aaaatagata
    30241 gcctctaaca tgtttgtttt tccttgtgca ttacgtccca aaaagacatt caatttagga
    30301 ttaaagtcta ttttcgtttc tttgtaatta cgaaaagtct tgagagatag atgttgtagc
    30361 cacatgatta tctaccaggg aatcgtggag cttgttggct tttaggtgat gaagcaggtt
    30421 tcgatttgtc ttttttaact cccttattca tctttttgac aagtttagcg atccgttctt
    30481 tttcaacttt atcagcttgg tattcatctt gttcttcaga agtaggttgt gtcaacaaga
    30541 tgtcaatgtt catgtcaggg atgtcaactt tatcgccaat acgaagtttt tttccacgac
    30601 ggctctctaa ttccctatta aagtaaacag aatgttcaga gagaaatgat ttaatagctc
    30661 ctccgctatg tgtaattcca agttctttga atagtgcttg gagggtaata aattcttcaa
    30721 ataatttgta ttccataatt cacctcaatc tttggtatta taccatattt tcctaaaaat
    30781 agtgagagag acagcgagaa atgtaagcga tttttatttc aaaagtgtta cagggaacaa
    30841 gaaaatttca ggttttcgtg atataataga agtctgtata taaggaggta aatcatggag
    30901 ttagtgcatg gaatttcaac acattttatc caatcaaaaa agtttaaaac aaacaaaatt
    30961 accgtgcgtt ttaccgctcc attatccctt gatacgattg caggtcacat gttgagtgca
    31021 agtatgctag agactgctaa tcagatgtac cccacttctc aagatttgag gagacacttg
    31081 gccagtctat acggtacaga tatgtcaacc aattgtttca gaagagggca aagccacatt
    31141 atagaattga catttaccta tgttcgtgat gagtttttaa gtaggaaaaa cgtgctaacc
    31201 tctcagattt tggaacttgt aaaagaaact cttttttcac ccgtagtagt tgataatggg
    31261 tttgatccgg ccttatttga aattgagaaa aaacaattgc tagcaagttt agcagctgat
    31321 atggatgatt ctttttattt tgcacataaa gaattggata aattgttttt tcatgatgaa
    31381 cgtcttcaat tggaatatag tgatttacga aatcgtattt tagctgaaac tccacaaagt
    31441 tcttattctt gtttccaaga atttttagcc aatgatcgaa tagatttctt tttcctaggt
    31501 gattttaatg aggttgaaat tcaaaatgta ttagaatcat ttggctttaa aggtcgaaaa
    31561 ggagatgtga aggttcagta ttgtcaacct tattctaata tccttcagga aggtatggtt
    31621 cggaaaaatg tgggacaatc cattttggaa ttaggttatc attactgttc taaatatggt
    31681 gatgagcaac atttacccat gattgtaatg aatggtttac ttggtggatt tgctcactct
    31741 aagctcttta caaatgtccg tgaaaatgct ggattagctt ataccatttc aagtgagctt
    31801 gatttattta gtggattctt gaggatgtat gctggtatca atcgagaaaa tcgtaaccag
    31861 gctcgtaaaa tgatgaataa tcaactgctt gatttaaaaa aaggttattt tacagagttt
    31921 gagttaaatc agaccaagga aatgattcgt tggtcgttgt tactttctca agataatcaa
    31981 tcttcattga ttgaacgtgc ttatcaaaat gccttatttg gaaaatcttc agcagacttt
    32041 aaaagttgga ttgcaaagct tgaacaaatt gacaaagatg ctatttgtag agtagctaat
    32101 aatgtgaaac tacaagcgat ttactttatg gaaggaatag aatgacaaag gttgtttttg
    32161 aagaaaaata ctatccagct gtaaaagaaa tggtttatcg aactcgtttg gccaacggat
    32221 tgacagttgc tcttttgcct aaaaaggaat ttaaagaggt ttacgggagt gtcactgtac
    32281 agtttggttc ggtagatacg tttgtcacag aagttgacgg agatgtaaaa caatatcctg
    32341 gaggaattgc tcattttctt gaacataaat tatttgagag agaagattct agtgatttga
    32401 tgtcggcttt tacgagtcta ggtgcagata gtaatgcctt tacaagcttt acaaaaacaa
    32461 actatctttt ttcagcaacg gattattttt tagaaaattt agatttactt gatgaattgg
    32521 taacatcagc acactttact gaagcttcca ttctgacaga gcaggatatt attcagcaag
    32581 aacgagaaat gtaccaagat gatccagatt cgtgtttatt cttttcaact ttagcgaatt
    32641 tgtatcctgg tacaccttta gcaactgata tagttggaag tgaggagtcc atttcccaaa
    32701 tcaatctaac taatttgcaa gaaaatttta caaagtttta caaacctgta aacatgtctc
    32761 tgtttttagt tggtaatttt gatgtggagc gagtacagga ctattttgaa agcaaagaac
    32821 tgaaagattc agattttcag gaagtagcaa gagaaaagtt gtttttacag cctgtaaagc
    32881 caacagatag tatgagaatg gaagtatctt ctcccaaact agcgattgga gttagaggta
    32941 agcgagaagt ttctgaagcg gattgctatc gacatcatat tttattaaaa ttattgtttg
    33001 caatgatgtt tggttggact tcggatcgtt ttcaaaaatg ttatgaatca ggtaaaattg
    33061 atgcgtcctt atctctggaa gttgaaataa caagtcgctt tcattttgtc atgttgacaa
    33121 tggatacgaa agagccagtt gctttgtctc atcaatttag gaaggctatt cgtaatttta
    33181 caaaggattt agatattaca gaggaacatt tagatattat caaaagagag atgtttggcg
    33241 aatttttcag tagcatgaac tctcttgaat ttattgcaac gcaatatgat gcttttgaaa
    33301 atggtgagat aatttttgat ttgccgaaaa ttttacagga aattacttta gaggatgtcc
    33361 ttgatgctgg acatcattta atagatgatg gtgacatagt tgattttaca atattcccat
    33421 cgtagtaacc tattataata gacactagaa agaagggatg acaagtatga gaaaaaaaac
    33481 aattggagag gttttacgat tagctagaat caatcaggga ttgagtttag atgaattgca
    33541 gaaaaagaca gaaatccagt tagatatgtt ggaagcaatg gaagcagacg atttcgatca
    33601 acttccaagt cctttttaca cgcgttcttt cttgaaaaaa tatgcatggg ctgttgagtt
    33661 agatgaccaa attgttttgg atgcttatga ttctgggagt atgattactt atgaggaagt
    33721 agatgttgat gaagatgagt tgacaggtcg tagacgttca agtaagaaaa agaagaaaaa
    33781 aacatcattt ttacctttat tttattttat cctttttgct ttatcgattt taatttttgt
    33841 gacttattat gtttggaact atattcaaac tcaaccagag gagccttctc tttctaatta
    33901 cagtgtggtt caatcaacaa gttcaactag ctctgttccc cactcctcaa gtagtagttc
    33961 ttctagtata gaatcagcta taagtgtatc aggcgaagga aatcatgtag aaatcgctta
    34021 taagacaagt aaggaaacag ttaaattgca attggcagtt tcagatgtta caagttgggt
    34081 cagtgtttca gaaagcgaac ttgagggcgg tgtaacctta tcgccaaaga agaaaagtgc
    34141 agaagcaaca gttgcaacta aaagtcctgt aacaattacg ttaggtgttg taaaaggtgt
    34201 tgatttgaca gtagataatc agactgttga tttatcgaaa ttaacagctc agactggaca
    34261 aatcactgta acctttacta aaaattaagg aaaaacgaat gaaaaaagaa caaattccca
    34321 atctcttaac aataggtcga attctcttta tacctatttt tatctttatt ttaacgatag
    34381 gaaattcgat agagagtcat atagttgcag ctattatctt tgctgttgcc agtattaccg
    34441 actatttaga tggatattta gctcgtaaat ggaatgtggt cagtaatttt ggtaaatttg
    34501 cagatcctat ggcggataag ttactagtta tgtcggcttt tattatgttg attgagttag
    34561 gtatggctcc ggcttggatt gttgcagtga ttatctgtcg tgagttagct gtgacaggtt
    34621 taaggctttt attggttgaa actggtggaa caattttagc agcagcaatg cctggaaaaa
    34681 ttaaaacttt tagtcagatg tttgccatta ttttcttgct attacattgg actttgcttg
    34741 gtcaagttct actttatgta gccttatttt tcactatcta ctctggctat gactatttca
    34801 agggtagtgc ctatgtattt aaagggacat ttggttcgaa atgaaatcaa taattgatgt
    34861 aaaaaatctt tcttttcgct ataaagaaaa tcagaactac tacgatgtga aggatattac
    34921 gtttcacgtg aaacgtggag aatggctttc gattgtaggg cataatggta gtggtaaatc
    34981 aacgacggtt cgattaattg atggcttact ggaagcagaa tccggagaga ttgtaattga
    35041 tggccaacgt ttgactgagg aaaatgtttg gaatatacgt cgtcaaatcg gtatggtttt
    35101 tcaaaatcca gacaatcaat ttgttggagc gactgttgaa gatgatgttg cttttggttt
    35161 ggaaaatcag ggactttctc gtcaagaaat gaaaaagaga gtggaagaag ctctggcttt
    35221 agttggcatg ttggacttta aaaagagaga gccagcgcgt ctatcaggtg gccaaaagca
    35281 acgtgtggcc attgcaggtg ttgtagccct aagaccagct attttaatct tagatgaagc
    35341 aacgagtatg ttggatcctg aggggcgtag agaacttatt gggacagtaa aaggaattcg
    35401 aaaagactat gatatgacgg tcatttctat tacccatgat ttggaagaag tcgccatgag
    35461 tgatcgcgta ttggtcatga aaaaagggga aattgaatca actagtagtc caagggagct
    35521 tttctctcga aatgatttag atcaaattgg attagacgat ccttttgcta atcaattaaa
    35581 aaaatctttg agccagaatg gctatgattt acctgaaaat tatttgacag aaagtgagct
    35641 agaggataag ctatgggaat tgctctagaa aatgtgaatt ttacatatca agaaggtact
    35701 cccttagctt cagcagcttt gtcggatgtt tctttgacga ttgaagatgg ctcttataca
    35761 gctttaattg ggcacacagg tagtggtaaa tcaactattt tacaactctt aaatggttta
    35821 ttggtgccaa gtcaagggag tgtgagggtt tttgatacct taatcacctc gacttctaaa
    35881 aataaagata ttcgtcaaat tagaaaacag gttggcttgg tatttcagtt tgctgaaaat
    35941 cagatttttg aagaaacggt tttgaaggac gttgcttttg gaccgcaaaa ttttggagtt
    36001 tctgaagaag atgctgtgaa gactgcgcgt gagaaactgg ctctggttgg aattgatgaa
    36061 tcactttttg atcgtagtcc gtttgagctg tcagggggac aaatgagacg tgttgccatt
    36121 gcaggcatac ttgccatgga gccagctata ttagtcttag atgagccaac agctggtcta
    36181 gatcctctag ggagaaaaga gttgatgacc ctgttcaaaa aactccacca gtcagggatg
    36241 accatcgtct tggtaacgca tttgatggat gatgttgctg aatatgcgaa tcaagtctat
    36301 gtaatggaaa agggacgttt agtaaagggg ggcaaaccaa gtgatgtctt tcaagacgtt
    36361 gtttttatgg aagaagttca gttgggagta cctaaaatta cggccttttg taaacgattg
    36421 gctgatagag gcgtgtcatt taaacgatta ccgattaaga tagaggagtt caaggagtcg
    36481 ctaaatggat agtatgattt tagggcgtta tatcccaggg gattcgattg ttcaccgatt
    36541 ggatccacgt agcaaattgc ttgctatgat gctactgatt ttgattgtgt tttgggctaa
    36601 taaccccttg acgaatctga ttctttttat agcgacaggg atatttattg ccttgtcagg
    36661 agtatctctt tcatttttta ttcagggctt gaagtctatg tttttcttga ttgccttcac
    36721 aactattttt caactatttt tcatttctaa tgggaatgtt ttatttgagt tttcgtttgt
    36781 gagaatcacg gattatgctt tgcaacaagc tgggattatt ttttgtcgct ttgtattgat
    36841 tattttcttt tcaactttgt taaccttaac aaccatgcct ttaagtttgg catcagctgt
    36901 cgaagcttta ttagcacctt taaagcgtgt gaaagttcca gttcatgaaa ttggattgat
    36961 gctgtctatg agtttgcgtt ttgtcccaac cttgatggat gatacgactc ggattatgaa
    37021 tgcacagaaa gctcgtggag tggattttgg agaaggaagc atcgttcaaa aagtaaaggc
    37081 gatgattccc attttgattc ctctttttgc gacaagttta aaacgtgcag attccttggc
    37141 tatcgctatg gaagcgcgtg gctatcaggg tggaaaaggc agaagtcaat accgacaatt
    37201 gaaatggact ctaaaggata cgctgaccat tcttgttatt ctcgtacttg gttgttgttt
    37261 atttttctta aaatcttagt agctttcagg aattgataaa aagttactgt aacagttttt
    37321 gatataaagg tagggatatg aaccgtttta aaaaatcaaa atatgtcatt attgtttttg
    37381 tcactgttct gcttgtgtca gctctcttag cgacgactta ttcaagtaca attgtgacaa
    37441 aattaggaga tggaatctca ttggttgata gagttgtaca aaaacctttt cagtggtttg
    37501 attctgtcaa atcagatttg gctcatttga cacgaacata taatgaaaat gaaagtttga
    37561 agaaacagct ttaccaatta gaagttaaat caaatgaggt ggaaagttta aagacagaaa
    37621 atgaacaact gcgccaattg cttgatatga agtctaaatt gcaagccaca aagactttag
    37681 cagcagatgt tattatgcgt tctccggtat cttggaagca ggagttgacc ttagatgcag
    37741 gtagatcaaa aggtgcttct gagaacatgt tagctattgc aaatggtggc ttgattggga
    37801 gtgtttcaaa agtagaggag aactctacta tagtcaacct tctgacaaat acggaaaatg
    37861 ctgataagat ttctgttaaa attcaacatg gctctactac aatttatgga attattattg
    37921 gctatgacaa ggaaaatgac gttcttaaaa ttagccaatt aaatagtaat agcgatatta
    37981 gtgcgggaga taaggtgact actggtggat taggaaactt taacgttgct gatattcctg
    38041 ttggtgaagt ggttgccaca acgcatagta cagactattt gacacgagaa gtaactgtta
    38101 aattgagtgc agatactcat aatgtagatg tgatagaatt agtggggaat tcataatgag
    38161 acagttgaag cgagttggag tatttttatt gcttcctttc tttgttctaa ttgacgccca
    38221 tattagccag cttctgggct catttttccc ccatgtacat ttggctagtc attttctttt
    38281 tctatttctc ttatttgaga cgatagaagt atcagagtat ctctacctag tctattgttt
    38341 tgttataggc ttggtttatg atgtttactt tttccatcta atagggatta caactctctt
    38401 atttatctta ttgggagcct tccttcataa attgaatagt gttattttgt tgaatcgttg
    38461 gacaagaatg ctagctatga ttgtgctgac attcctgttt gaaatgggta gttatctttt
    38521 ggcttttatg gtagggttga cagtagatag catgtcgatt tttatagtct atagcttggt
    38581 accgacgatg attttaaatt ttttatggat tactgttttt caatttattt ttgaaaaata
    38641 ttatctataa gaacgacata taaatgtaac aaaggcgtaa tatttattag gccttttttt
    38701 ggtatactag tattgtcttt aaaagaagga gtatctacgt aatatgaaga aaaaaatctt
    38761 agcgtcactt ttattaagta cagtaatggt ttctcaagta gctgttttaa caactgcgca
    38821 tgcagaaacg actgatgaca aaattgctgc tcaagataat aaaattagta acttaacagc
    38881 acaacaacaa gaagcccaaa aacaagttga ccaaattcag gagcaagtat cagctattca
    38941 agctgagcag tctaacttgc aagctgaaaa tgatagatta caagcagaat ctaagaaact
    39001 cgagggtgag attacagaac tttctaaaaa cattgtttct cgtaaccaat cgttggaaaa
    39061 acaagctcgt agtgctcaaa caaatggagc cgtaactagc tatatcaata ccattgtaaa
    39121 ctcaaaatca attacagaag ctatttcacg tgttgctgca atgagtgaaa tcgtatctgc
    39181 aaacaacaaa atgttagaac aacaaaaggc agataaaaaa gctatttctg aaaaacaagt
    39241 agcaaataat gatgctatca atactgtaat tgctaatcaa caaaaattgg ctgatgatgc
    39301 tcaagcattg actacgaaac aggcagaact aaaagctgct gaattaagtc ttgctgctga
    39361 gaaagcgaca gctgaagggg aaaaagcaag tctattagag caaaaagcag cagctgaggc
    39421 agaggctcgt gcagctgcgg tagcagaagc agcttataaa gaaaaacgag ctagccaaca
    39481 acaatcagta cttgcttcag caaacactaa cttaacagct caagtgcaag cagtatctga
    39541 atctgcagca gcacctgtcc gtgcaaaagt tcgtccaaca tacagtacaa acgcttcaag
    39601 ttatccaatt ggagaatgta catggggagt aaaaacattg gcaccttggg ctggagacta
    39661 ctggggtaat ggagcacagt gggctacaag tgcagcagca gcaggtttcc gtacaggttc
    39721 aacacctcaa gttggagcaa ttgcatgttg gaatgatggt ggatatggtc acgtagcggt
    39781 tgttacagct gttgaatcaa caacacgtat ccaagtatca gaatcaaatt atgcaggtaa
    39841 tcgtacaatt ggaaatcacc gtggatggtt caatccaaca acaacttctg aaggttttgt
    39901 tacatatatt tatgcagatt aatttacaga gggactcgaa tagagccctc ttttcaggtt
    39961 ttaccgtgac aatccctatt aaaaattata tcaaaatagc ttgaaaatat tggaaaagta
    40021 tggtagaatg aaaattgtcg tgtgaacgat aatactcatt cttgatgaat tgtgaagcag
    40081 ttgcccttgg gtcgttttgc gagttgaagt caagaagagg aaaaaaacaa aaaggagaaa
    40141 tactcatggc agtaatttca atgaaacaac ttcttgaggc tggtgtacac tttggtcacc
    40201 aaactcgtcg ctggaatcct aagatggcta agtacatctt tactgaacgt aacggaatcc
    40261 acgttatcga cttgcaacaa actgtaaaat acgctgacca agcatacgac ttcatgcgtg
    40321 atgcagcagc taacgatgca gttgtattgt tcgttggtac taagaaacaa gcagctgatg
    40381 cagttgctga agaagcagta cgttcaggtc aatacttcat caaccaccgt tggttgggtg
    40441 gaactcttac aaactgggga acaatccaaa aacgtatcgc tcgtttgaaa gaaattaaac
    40501 gtatggaaga agatggaact ttcgaagttc ttcctaagaa agaagttgca cttcttaaca
    40561 aacaacgtgc gcgtcttgaa aaattcttgg gcggtatcga agatatgcct cgtatcccag
    40621 atgtgatgta cgtagttgac ccacataaag agcaaatcgc tgttaaagaa gctaaaaaat
    40681 tgggaatccc agttgtagcg atggttgaca ccaatactga tccagatgat atcgatgtaa
    40741 tcatcccagc taacgatgac gctatccgtg ctgttaaatt gatcacagct aaattggctg
    40801 acgctattat cgaaggacgt caaggtgagg atgcagtagc agttgaagca gaatttgcag
    40861 ctttagaaac tcaagcagat tcaattgaag aaatcgttga agttgtagaa ggcgacaacg
    40921 cttaatttat acaaatagta attacctagg agggcggggc ttagcccggc tctcctattt
    40981 tcaaaaaata taggagaatt aaaatggcag aaattacagc taaacttgta aaagagttgc
    41041 gtgaaaaatc tggtgccggt gttatggacg ctaaaaaagc gcttgtagaa acagacggtg
    41101 acatcgaaaa agcgattgaa ttgcttcgtg aaaaaggtat ggctaaggca gctaagaaag
    41161 ctgaccgtgt tgctgcagaa ggtttgactg gtgtttatgt taacggtaat gttgcagcag
    41221 ttattgaagt aaacgctgaa actgacttcg ttgcaaaaaa cgctcaattc gttgaattgg
    41281 taaatactac agctaaagtc attgctgaag gaaaacctgc taacaacgaa gaagctcttg
    41341 ctttgataat gccttcaggt gaaactcttg aagctgcata cgtatctgca acagcaacta
    41401 tcggagagaa aatctcattc cgtcgctttg cattgattga aaaaacagac gcacaacact
    41461 ttggagcata ccaacataac ggtggacgta tcggtgttat ttcagttgtt gaaggtggag
    41521 acgaagcact tgctaaacaa ttgtcaatgc acatcgcagc gatgaaacca acagttcttt
    41581 cttacaaaga attggatgag caattcgtta aagatgagtt ggcacaattg aatcacgtta
    41641 tcgaccaaga caacgaaagc cgtgcaatgg ttaataaacc agctcttcca cacttgaagt
    41701 atggatcaaa agctcaatta actgatgatg ttattgctca agctgaagct gacatcaaag
    41761 ctgaattggc tgcagaaggc aaaccagaaa aaatctggga caaaattatt ccaggtaaaa
    41821 tggatcgctt catgcttgat aacactaaag ttgaccaagc ttacacactt cttgcacaag
    41881 tttacatcat ggatgacagc aagacagttg aagcatacct tgaatcagtt aacgcttcgg
    41941 tagttgagtt tgctcgcttt gaagttggtg aaggtatcga gaaagctgca aacgacttcg
    42001 aagctgaagt tgcagctaca atggcagcag ccttgaataa ctaataatag aaaggaagca
    42061 aattgcttcc ttttttgtat agggcttaga tttttcctga aattgttttt tccccagtct
    42121 cactcgatta gaataattaa aacaaaaagc cctataataa gggctaagtg catttaggag
    42181 actatacttc aaattcatag taagcgcgtc ataacaaggt atctatccta attccccaac
    42241 ttccagtata ccagataaaa aataattatc ggaggaaagg tattgtacta tgataattca
    42301 actaagtgat ttaggtcaag ttcaccttgt ttgtggcaag acagatatga ggcagggaat
    42361 agactcatta gcctatgtag ttaaaaccca ctttgaattg gatcctttct ccgatcaagt
    42421 ctttctcttt tgtggtggac gtaaagaccg ctttaaagtt ctttactggg atggtcaagg
    42481 attttggata ttatataaac gctttgagaa cggaaaactg acttggtcca gtacagaaaa
    42541 ggatgtcaaa gctctcgcac ctgaacaagt agattggctg atgaagggct tttctatcac
    42601 tccccaaata tagtggattt aaactagaat agtacacctc tgcttctaaa acattgttag
    42661 aaatcgattt gactatcctg ttcttatttc attttactat aaattcatca gaaagtcgtg
    42721 atttctattg aaatgaggac tttcttttta tactcatctg ttttcaaaaa gcattctagt
    42781 ccgtctccga ttaacgatgg actttatcac ctccttctcc agtccttgta tgacatcttc
    42841 caaagttcga aaggctttat tcttaaatcc acgtttacga atctctttcc acacttgttc
    42901 aat
//
LOCUS       DAVKNL010000015        40273 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVKNL010000015 DAVKNL010000000
VERSION     DAVKNL010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40273
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.015"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            5..75
                     /locus_tag="VW411_000735"
     tRNA            5..75
                     /locus_tag="VW411_000735"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37..39,aa:Gly,seq:tcc)
     gene            110..183
                     /locus_tag="VW411_000736"
     tRNA            110..183
                     /locus_tag="VW411_000736"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:144..146,aa:Ile,seq:gat)
     gene            266..355
                     /locus_tag="VW411_000737"
     tRNA            266..355
                     /locus_tag="VW411_000737"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:302..304,aa:Ser,seq:tga)
     gene            366..439
                     /locus_tag="VW411_000738"
     tRNA            366..439
                     /locus_tag="VW411_000738"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:400..402,aa:Met,seq:cat)
     gene            443..515
                     /locus_tag="VW411_000739"
     tRNA            443..515
                     /locus_tag="VW411_000739"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:476..478,aa:Phe,seq:gaa)
     gene            528..608
                     /locus_tag="VW411_000740"
     tRNA            528..608
                     /locus_tag="VW411_000740"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:562..564,aa:Tyr,seq:gta)
     gene            613..683
                     /locus_tag="VW411_000741"
     tRNA            613..683
                     /locus_tag="VW411_000741"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:645..647,aa:Trp,seq:cca)
     gene            698..770
                     /locus_tag="VW411_000742"
     tRNA            698..770
                     /locus_tag="VW411_000742"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:731..733,aa:His,seq:gtg)
     gene            781..852
                     /locus_tag="VW411_000743"
     tRNA            781..852
                     /locus_tag="VW411_000743"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:813..815,aa:Gln,seq:ttg)
     gene            862..945
                     /locus_tag="VW411_000744"
     tRNA            862..945
                     /locus_tag="VW411_000744"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:896..898,aa:Leu,seq:caa)
     gene            1052..2329
                     /locus_tag="VW411_000745"
     CDS             1052..2329
                     /locus_tag="VW411_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEU9793509.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     MKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDETDFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATSEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            2405..3889
                     /locus_tag="VW411_000746"
     CDS             2405..3889
                     /locus_tag="VW411_000746"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU9793510.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPDTYPKVDLNF
                     DKLKDASYQEVAKLVLSAFLDDFTVEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFANVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTTELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVCELDS
                     YIEDPHTAVASAVYKKYQSATGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            3950..5230
                     /locus_tag="VW411_000747"
     CDS             3950..5230
                     /locus_tag="VW411_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU9793511.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTIVSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            5234..5854
                     /locus_tag="VW411_000748"
     CDS             5234..5854
                     /locus_tag="VW411_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9793512.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            6020..7132
                     /locus_tag="VW411_000749"
     CDS             6020..7132
                     /locus_tag="VW411_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU9793513.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEVTKTVIASEEVAPST
                     GTSVPEEQTTETTRPVEEATPQETTPAEKQETQASPQAASAVEVTTTSSEAKEVASSN
                     GATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAAFKEEIANLFG
                     ITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGISYIIWKQRFY
                     APFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            7455..7880
                     /locus_tag="VW411_000750"
     CDS             7455..7880
                     /locus_tag="VW411_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEU9793514.1"
                     /translation="MLEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAVQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIRVKEEIESILTEFERTELSRLLNKLVLGIENIEI"
     gene            7891..8250
                     /locus_tag="VW411_000751"
     CDS             7891..8250
                     /locus_tag="VW411_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEU9793515.1"
                     /translation="MSIILTTIVALEHFYIFYLESIATQSDATSRVFNMEKEELAHPS
                     VSSLFKNQGIYKALLGVFLLYVIYFSQNLEIVTIFVLFVIGAATYGSLTADKKIILKQ
                     GGSAILALISILLFKYT"
     gene            8357..9001
                     /gene="pcp"
                     /locus_tag="VW411_000752"
     CDS             8357..9001
                     /gene="pcp"
                     /locus_tag="VW411_000752"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEU9793516.1"
                     /translation="MKVLVTGFEPFGGEKGNPALEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSLTPERVTINQDDACISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKSPYVK
                     AGFMHIPYMMEQVVNRPTTPAMSLVDIRRGIEAAIGAIIEHGDQELKLVGGETH"
     gene            complement(9029..9886)
                     /locus_tag="VW411_000753"
     CDS             complement(9029..9886)
                     /locus_tag="VW411_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEU9793517.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSGFLNP
                     LSRIHGTVLEQPFFSILGQILQTYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            10016..11158
                     /gene="tgt"
                     /locus_tag="VW411_000754"
     CDS             10016..11158
                     /gene="tgt"
                     /locus_tag="VW411_000754"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU9793518.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            11256..13073
                     /locus_tag="VW411_000755"
     CDS             11256..13073
                     /locus_tag="VW411_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEU9793519.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAVAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQESKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPIVQTLRPLPYIHAILAAGTGILTIIVCTVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            13226..14377
                     /gene="nagA"
                     /locus_tag="VW411_000756"
     CDS             13226..14377
                     /gene="nagA"
                     /locus_tag="VW411_000756"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEU9793520.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAIDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            14540..15598
                     /locus_tag="VW411_000757"
     CDS             14540..15598
                     /locus_tag="VW411_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEU9793521.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPATFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            15749..16114
                     /locus_tag="VW411_000758"
     CDS             15749..16114
                     /locus_tag="VW411_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEU9793522.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            16190..17131
                     /locus_tag="VW411_000759"
     CDS             16190..17131
                     /locus_tag="VW411_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEU9793523.1"
                     /translation="MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDHKIASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAS
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            17079..18095
                     /locus_tag="VW411_000760"
     CDS             17079..18095
                     /locus_tag="VW411_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU9793524.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSALLDKQCVTQMRVGLSQGKSFSEMMESLGCSSAIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEVGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            18097..18423
                     /gene="comGC"
                     /locus_tag="VW411_000761"
     CDS             18097..18423
                     /gene="comGC"
                     /locus_tag="VW411_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="comG operon protein ComGC"
                     /protein_id="HEU9793525.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLEKNEDASLRKLQADGRITEEQAKAYKEYNDKNGGA
                     NRKVND"
     gene            18416..18820
                     /locus_tag="VW411_000762"
     CDS             18416..18820
                     /locus_tag="VW411_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEU9793526.1"
                     /translation="MIKAFTMLESLLVLGLVSILALGLSGSVQSTFSAVEEQIFFMEF
                     EELYRETQKRSVASQQKTSLNLDGQTLSNGSQKLPVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            18825..19085
                     /locus_tag="VW411_000763"
     CDS             18825..19085
                     /locus_tag="VW411_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793527.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            19048..19509
                     /locus_tag="VW411_000764"
     CDS             19048..19509
                     /locus_tag="VW411_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU9793528.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEVELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTNARGRGYQPMVYGLKSVRITEDNQLVRFHFQFQKGLEREFIYRVEKEKS"
     gene            19487..19900
                     /locus_tag="VW411_000765"
     CDS             19487..19900
                     /locus_tag="VW411_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU9793529.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQVAHYQDYALNK
                     EKLVAFAMAKRTKDKVEQESGEQFFNLGQVSYQNKKTGLVTRVRTDKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKVEKKKSEEKPEKKENS"
     gene            20037..20631
                     /locus_tag="VW411_000766"
                     /pseudo
     CDS             20037..20631
                     /locus_tag="VW411_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            20692..21645
                     /locus_tag="VW411_000767"
     CDS             20692..21645
                     /locus_tag="VW411_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9793530.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKENNQTLKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGAIFLTSLTKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKNEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            21696..22886
                     /locus_tag="VW411_000768"
     CDS             21696..22886
                     /locus_tag="VW411_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEU9793531.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHIQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGADAIVFTAGVGE
                     NAESFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            23035..23406
                     /gene="rnpA"
                     /locus_tag="VW411_000769"
     CDS             23035..23406
                     /gene="rnpA"
                     /locus_tag="VW411_000769"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEU9793532.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNRFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            23381..24205
                     /locus_tag="VW411_000770"
     CDS             23381..24205
                     /locus_tag="VW411_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU9793533.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            24224..25210
                     /locus_tag="VW411_000771"
     CDS             24224..25210
                     /locus_tag="VW411_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEU9793534.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QAESQELEDLGLKVETNFDIEQVATEVMAYVQTIIDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSRTFYVTINVNDYVEHRAEVLQTYAQKL
                     ATRVLEEGRSHKTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            25261..25884
                     /locus_tag="VW411_000772"
     CDS             25261..25884
                     /locus_tag="VW411_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEU9793535.1"
                     /translation="MLNKIRDYLDFAGLQYRNPDKAGAEREKMLAFRHKGQEARKVFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            26015..27472
                     /locus_tag="VW411_000773"
     CDS             26015..27472
                     /locus_tag="VW411_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9793536.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIVAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            27495..27776
                     /locus_tag="VW411_000774"
     CDS             27495..27776
                     /locus_tag="VW411_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9793537.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            27853..28338
                     /locus_tag="VW411_000775"
     CDS             27853..28338
                     /locus_tag="VW411_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9793538.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            28355..29020
                     /locus_tag="VW411_000776"
     CDS             28355..29020
                     /locus_tag="VW411_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEU9793539.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGIDVFTFIAGRGITEAVDPAGAARAFKDEIKR
                     IWG"
     gene            29024..29887
                     /locus_tag="VW411_000777"
     CDS             29024..29887
                     /locus_tag="VW411_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEU9793540.1"
                     /translation="MVRPIGIYEKATPTHCTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKAIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIS
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            29889..30593
                     /locus_tag="VW411_000778"
     CDS             29889..30593
                     /locus_tag="VW411_000778"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEU9793541.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKTWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            30772..32445
                     /locus_tag="VW411_000779"
     CDS             30772..32445
                     /locus_tag="VW411_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9793542.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYNLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            32557..33648
                     /gene="ulaG"
                     /locus_tag="VW411_000780"
     CDS             32557..33648
                     /gene="ulaG"
                     /locus_tag="VW411_000780"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEU9793543.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            33762..35738
                     /gene="tkt"
                     /locus_tag="VW411_000781"
     CDS             33762..35738
                     /gene="tkt"
                     /locus_tag="VW411_000781"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU9793544.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDANRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            35855..36154
                     /gene="yajC"
                     /locus_tag="VW411_000782"
     CDS             35855..36154
                     /gene="yajC"
                     /locus_tag="VW411_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU9793545.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            36200..36628
                     /locus_tag="VW411_000783"
     CDS             36200..36628
                     /locus_tag="VW411_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEU9793546.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            36630..37040
                     /locus_tag="VW411_000784"
     CDS             36630..37040
                     /locus_tag="VW411_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793547.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            37340..39991
                     /gene="adhE"
                     /locus_tag="VW411_000785"
                     /gene_synonym="adhC"
     CDS             37340..39991
                     /gene="adhE"
                     /locus_tag="VW411_000785"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEU9793548.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRKLAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT        12216 a         7220 c         9076 g        11761 t
ORIGIN      
        1 tttagcgggt gtagtttagt ggtaaaacta cagccttcca agctgttgtc gcgagttcga
       61 ttctcgtcac ccgctttgaa ctttgttctt tgtaccaagt ttttgacttg ggcgcgtagc
      121 tcaggtggtt agagcgcacg cctgataagc gtgaggtcgg tggttcgagt ccactcgtgc
      181 ccatagtgtt tagtccatta ctaggggatt ggaatattat ctgttcacta agaggacacg
      241 ggcttgttcc cgtataaact attttggagg attacccaag tccggctgaa gggaacggtc
      301 ttgaaaaccg tcaggcgtgt aaaagcgtgc gtgggttcga atcccacatc ctccttttat
      361 attaacgcgg gatggagcag ctcggtagct cgtcgggctc ataacccgaa ggtcgtaggt
      421 tcaaatcctg ctcccgcaat aaggctcggt agctcagttg gtagagcaat ggattgaagc
      481 tccatgtgtc ggcggttcga ttccgtctcg cgccatttat atattttgga agggtagcga
      541 agaggctaaa cgcggcggac tgtaaatccg ctccttcggg ttcgggggtt cgaatccctc
      601 cccttccatt ttacgggcat agtttaaagg tagaactaag gtctccaaaa ccttcagtgt
      661 gggttcaatt cctactgccc gtgttaatag aattatggcg ggtgtggtga agtggttaac
      721 acaccagatt gtggctctgg catgcgtggg ttcgatcccc atcactcgcc tattttatat
      781 tggggtatag ccaagcggta aggcaaggga ctttgactcc ctcatgcgtt ggttcgaatc
      841 cagctacccc agttactatt tgccggcgtg gcggaattgg cagacgcgct ggactcaaaa
      901 tccagtgtcc gcaaggacgt gccggttcga ccccggccgc cggtatagta tagtgttagg
      961 aacgttgtta ttcttcgttc cttttttata ttatttttgg tataattata gttattcaaa
     1021 ttttatttag attaagaaag tgtaggggag tatgtcttgt tctatcgatt tattaaaaca
     1081 tcggtatttg aaaaatatta aagaaaatcc tgaattgttt gtcggaattg agttggagta
     1141 tcctgttgca agtttagaag gggatgctac agatgttgaa gttatgaagg atctatttca
     1201 ttatttagtt tctactttgg atctcaccgt agcaaaggta gatgattttg gcaatctgat
     1261 ccagttagta gatccgataa gtcaggatgc tattttattt gaagtttcct atacaacgat
     1321 tgagtttgca tttggtaagg ctgaaacgat tcaagaggtc gaaaatcgtt tcaataatta
     1381 tatgaatgta attcagagaa agttagctga atcaaatcat gctattgttg gctgtggtat
     1441 ccatcccaac tgggataaaa atgagaattg tccagtggct tatccacgct atcagatgtt
     1501 gatggattat ttgaatttga gtagaaatat tattaaatca gatttacatc atttccctga
     1561 atatggtact tttatctgtg ggagccaggt tcagctggat atttcaaaaa ccaactactt
     1621 acgggtgatt aatgctttta ctcaaattga agcggctaag gcttatttat ttgcaaactc
     1681 tgaattttcg ggtgcggatt gggatacgaa aatttcaagg gatattttct gggaagaatc
     1741 tatgcatggt atctatccag agaatgttgg ggtcaatgct agactcctta atgatgaaac
     1801 tgattttttt gactatctaa atcattctgc gatttttact gcggaacgtg atgggcagac
     1861 ctattatttt tatcctattc aggctgggga ctatttggct acgtccgaaa tccaagcatt
     1921 tgctctgaat ggggatgagg ttattattta cccccaagag aaggattttg aaactcatcg
     1981 tagttaccag taccaagatt taacgactcg aggaacagtt gagtttcgta gtgtgtgtac
     2041 acagccactt gataggactt ttgcttctgc agcttttcac ttgggattat tggttaattt
     2101 agacaagtta gaagcttact tagaaacagc acctttcttt aaagtatttg gttatgatta
     2161 caagtcttta aggagacaat tttctaagaa aaatcttaca gatgaggaag aaactacgat
     2221 tattgaattt tccaaagact tactcctact agctgaggag ggactagtgg tgagaaataa
     2281 ggaagaaatg acctatttac agcctttgag agaagaattg agcctataat ttctcttata
     2341 aagggagaat tttctgaaaa atcatgatat aatggacgag actatagata aaggatagag
     2401 agtaatgaca ttagtttatc aatcaacgcg tgatgccaac aatacagtaa ctgccagcca
     2461 agcaattttg caaggtttgg cgacggacgg cggtttgttt acaccggata cttatccaaa
     2521 ggtagatttg aactttgaca aattgaaaga tgcttcttac caggaagttg ctaagctagt
     2581 tttgtcagca tttttagatg actttacagt tgaggagttg gactactgta tcaacaatgc
     2641 ctacgatagc aaatttgata ctccagctat tgcaccatta gtgaaattag atgggcaata
     2701 caatttggaa cttttccatg gttcaacgat tgcctttaag gatatggcct tgtctatttt
     2761 gccatacttt atgacgactg ctgctaagaa acatggtttg gagaacaaga ttgttatctt
     2821 gacagcgaca tctggtgaca cggggaaagc tgctatggcg gggtttgcga atgtgcctgg
     2881 tactgagatt atcgtctttt atccaaagga tggtgtcagc aagattcaag agttacaaat
     2941 gaccactcag actggcgaca atactcatgt tattgctatt gatggtaact ttgacgatgc
     3001 gcaaacaaat gtgaagcaca tgtttaacga cgtggctctt cgtgaaaaat tgactaccaa
     3061 caagttgcaa ttttcatcag ctaactctat gaacattggt cgtctggtgc cacaaattgt
     3121 ttattatgtt tatgcttacg ctcaattggt taagactggt gaaattgtag ctggtgaaaa
     3181 ggttaacttc acagtaccaa caggaaactt tggaaatatc ttggctgcct tttatgccaa
     3241 acaaattggt ttgccagttg gtaaattaat ctgtgcttca aatgacaaca atgttttgac
     3301 agacttcttt aaaacacgtg tctatgacaa aaaacgtgag tttaaggtaa caaccagccc
     3361 atctatggat atcttggtat cttcaaactt ggagcgcttg attttccatc ttttgggaaa
     3421 taatgctgaa aagacaactg aacttatgaa tgccttgaac acgcaaggac aatataagtt
     3481 gacagacttt gatgcagaga ttttggacct ctttgcagct gaatatgcga ctgaggaaga
     3541 aacggcagca gagatcaagc gtgtttgtga gttagattct tatatcgagg accctcatac
     3601 agctgttgct tcagcagttt ataaaaaata ccaatcggcc actggagatg taactaagac
     3661 agtgattgct tcaacagcta gtccatacaa gttcccagta gttgcagtag aagctgtaac
     3721 tggaaaagca ggtttaacag actttgaagc cttggctcaa ttacatgaaa tctcaggcgt
     3781 tgcagtgcca ccagcagttg atgggcttga aatagctcca attcgtcaca agacaacagt
     3841 ggcagctgct gacatgcaag cagcggttga ggcttattta ggactttaag acagagggag
     3901 caaactcggt tgggaaacca actgagtttc ttttcatcag gaggagagat tgtttaagaa
     3961 aaataaagac attcttaata ttgcattgcc agctatgggt gaaaactttt tgcagatgct
     4021 aatgggaatg gtggacagtt atttggttgc tcatttagga ttgatagcta tttcaggggt
     4081 ttcagtagct ggtaatatta tcaccattta tcaggcgatt ttcatcgctc tgggagctgc
     4141 tatttccagt gttatttcaa aaagcatagg gcagaaagac cagtcgaagt tggcctatca
     4201 tgtgactgag gcgttgaaga ttaccttact attaagtttc cttttaggat ttttgtccat
     4261 cttcgctggg aaagagatga taggactttt ggggacggag agggatgtag ctgagagtgg
     4321 tggactgtat ctatctttgg taggcggatc gattgttctc ttaggtttaa tgactagtct
     4381 aggagccttg attcgtgcaa cgcataatcc acgtctgcct ctctatgtta gttttttatc
     4441 caatgccttg aatattcttt tttcaagtct agctattttt gttctggata tggggatagc
     4501 tggtgttgct tgggggacaa ttgtgtctcg tttggttggt cttgtgattt tgtggtcaca
     4561 attaaaactg ccttatggga agccaacttt tggtttagat aaggaactgt tgaccttggc
     4621 tttaccagca gctggagagc gacttatgat gagggctgga gatgtagtga tcattgcctt
     4681 ggtcgtttct tttgggacgg aggcagttgc tgggaatgca atcggagaag tcttgaccca
     4741 gtttaactat atgcctgcct ttggcgtcgc tacggcaacg gtcatgctgt tggcccgagc
     4801 agttggagag gatgattgga aaagagttgc tagtttgagt aaacaaacct tttggctttc
     4861 tctgttcctc atgttgcccc tgtcctttag tatatatgtc ttgggtgtac cattaactca
     4921 tctctatacg actgattctc tagcggtgga ggctagtgtt ctagtgacac tgttttcact
     4981 acttgggacc cctatgacga caggaacagt catctatacg gcagtctggc agggattagg
     5041 aaatgcacgc ctcccttttt atgcgacaag tataggaatg tggtgtatcc gcattgggac
     5101 aggatatctg atggggattg tgcttggttg gggcttgcct ggtatttggg cagggtctct
     5161 cttggataat ggttttcgct ggttatttct acgctatcgt taccagcgct atatgagctt
     5221 gaaaggatag gaaatgcaaa aaacagcttt tatttgggat ttagacggga ctttattgga
     5281 ctcttacgaa gcgattttat cagggattga ggagactttt gctcagtttt ctattcctta
     5341 tgataaggag aaggtgagag agtttatctt caagtattcg gtgcaagatt tgcttgtgcg
     5401 ggtggcagaa gatagaaatc tggatgttga ggtgctaaat caggtgcgtg cccagagtct
     5461 ggctgagaag aatgctcagg tagttttgat gccaggtgcg cgtgaggtgc tagcttgggc
     5521 agacgaatca ggaattcagc agtttatata tactcataag gggaacaacg cttttaccat
     5581 tctcaaggac ttgggggtgg aatcctattt tacagagatt ttaaccagtc agagtggctt
     5641 tgtgcggaag ccaagtccag aagcggctac ctatctgcta gataagtatc agttgaattc
     5701 tgataatact tattatatag gggatcggac tctggatgtg gaatttgccc agaatagtgg
     5761 gattcaaagc atcaactttt tagagtctac ttatgaaggg aatcacagga ttcaagcgtt
     5821 agcagatatt tcccgtattt ttgagactaa gtgataaaaa gattgtgtca gttttgtgac
     5881 agagacctaa caaactattt caagtaacct agtttgttac aaggaataga cagttctgtt
     5941 aaataggccc gagagggctt tttttctaca ttttttgtgt tatgatagac aggtactcat
     6001 ttgaaaggaa tttgaaagaa tgaagaaaag aatgttatta gcgtcaacag tagccttgtc
     6061 atttgcccca gtattggcaa ctcaagcaga agaagttctt tggactgcac gtagtgttga
     6121 gcaaatccaa aacgatttga ctaaaacgga caacaaaaca agttataccg tacagtatgg
     6181 tgatactttg agcaccattg cagaagcctt gggtgtagat gtcacagtgc ttgcgaatct
     6241 gaacaaaatc actaatatgg acttgatttt cccagaaact gttttgacaa cgactgtcaa
     6301 tgaagcagaa gaagtaacag aagttgaaat ccaaacacct caagcagact ctagtgaaga
     6361 agtgacaact gcgacagcag atttgaccac taatcaagtg accgttgatg atcaaactgt
     6421 tcaggttgca gacctttctc aaccaattgc agaagttaca aagacagtga ttgcttctga
     6481 agaagtggca ccatctacgg gcacttctgt cccagaggag caaacgaccg aaacaactcg
     6541 cccagttgaa gaagcaactc ctcaggaaac gactccagct gagaagcagg aaacacaagc
     6601 aagccctcaa gctgcatcag cagtggaagt aactacaaca agttcagaag caaaagaagt
     6661 agcatcatca aatggagcta cagcagcagt ttctacttat caaccagaag agacgaaaat
     6721 aatttcaaca acttacgagg ctccagctgc gcccgattat gctggacttg cagtagcaaa
     6781 atctgaaaat gcaggtcttc aaccacaaac agctgccttt aaagaagaaa ttgctaactt
     6841 gtttggcatt acatccttta gtggttatcg tccaggagac agtggagatc acggaaaagg
     6901 tttggctatc gactttatgg taccagaacg ttcagaatta ggggataaga ttgcggaata
     6961 tgctattcaa aatatggcca gccgtggcat tagttacatc atctggaaac aacgtttcta
     7021 tgctccattc gatagcaaat atgggccagc taacacttgg aacccaatgc cagaccgtgg
     7081 tagtgtgaca gaaaatcact atgatcacgt tcacgtttca atgaatggat aaacccgact
     7141 tgataacatc attttgacga atgagatcta gctttcgtga tggaaagcga ttctcgttcg
     7201 ttttttcttt gtcatactct tcgaaaatct cttcaaacca cgtcagtttt atctgaaact
     7261 tcaaagctgt gctttgagca acctgcgact agcttcctag tttgcttttt gattttcatt
     7321 gagtatcaat ttgaatggaa aatggaaagt tatcatcttg taatgagtta agcaacattc
     7381 ttgcaatcta ttttacttta tatcacaatt aattagtcaa atattgataa atcaataaaa
     7441 agagagggga agaaatgcta gagattcaag atttactgta tcaactccgc ttgtctgagc
     7501 aagcgagtac gcaattgttt gaaaaaaggc ttgggattag tttgacacgg tatcagattt
     7561 tactgttttt gctggagcat tctccttgta accaaatggc ggttcaggag cgtttgaaaa
     7621 ttgatcaggc tgctttgaca cggcatttca aaattttgga aacggaaggt ttggtggagc
     7681 gtcatcgtaa tcctgaaaat cagcgggaag tgttggtaga ggctgcgaag tatgccaagg
     7741 agcagttagt ggtgaatccc cctctgcaac atatcagggt taaggaagag atagaaagta
     7801 tcttaacaga gtttgagaga acagaactca gccgtttatt aaataaattg gttttgggta
     7861 ttgaaaatat agaaatttaa ggagaaatag atgtcaatta ttttaacaac gatcgttgct
     7921 ttggagcatt tttacatttt ttatttggaa agtattgcca cgcaatcaga tgcgactagt
     7981 cgtgtattta atatggaaaa ggaagaattg gctcatccgt cagtaagttc attgttcaaa
     8041 aatcaaggaa tttataaggc tctgctagga gtctttctct tgtatgtcat ttatttctca
     8101 cagaatttag aaattgtgac tatttttgtc ttatttgtga ttggtgctgc gacttacggc
     8161 tctttaacag cggataaaaa aattattttg aaacaaggtg gatcagctat tttggccttg
     8221 attagtattt tactctttaa atacacttga aggtcgattc taatctcgct aatccttttt
     8281 aatccagaat aagggaaata tgttatactt gtttttaaga aaaaagtctc attgaattgg
     8341 ttttgaggag ttagaaatga aagtattagt gacaggtttt gagccctttg gaggggaaaa
     8401 gggcaatcca gctttggagg ccattaaagg tttaccagct gaaatccatg gtgctgaggt
     8461 ccgttggcta gaggtgccga cagtttttca caaatctgct caagtattgg aagaagagat
     8521 gaatcgttat caacctgact ttgtcctttg tattgggcaa gctggtggaa gaactagttt
     8581 gacacctgaa cgagtgacca ttaatcaaga cgatgcatgc atttctgata acgaagataa
     8641 tcaaccgatt gaccgtccca ttcgcccaga tggtgcttcg gcctacttta gtagtttgcc
     8701 gattaaagcg atggttcaag ctataaaaaa agagggctta ccggcctctg tttccaatac
     8761 ggcagggact tttgtctgca gccatttgat gtatcaggct ctctatttgg tagaaaagaa
     8821 atctccatat gttaaggcag gttttatgca tattccttat atgatggaac aggtggtgaa
     8881 cagaccgact actccagcta tgagtttagt ggatattcgg cgagggatag aagcagcaat
     8941 cggcgctata atagaacatg gagatcagga actcaagttg gtaggcggag aaactcattg
     9001 atagaaaaaa gcttgagggg aaaaaccttc aagcttttgg acgttttcgg gccaatactg
     9061 ctcggtaaaa cataatttta gtgcattgga tataaggtag gagtgaaaaa ctagcaatgc
     9121 caaaggtaat ccaattgagg aagtaccaag gaagaagctg taaatctagg acaaagtgct
     9181 ggaacttgta gcccttcata aaggaacggc tagtttttag gattcgtctt ggtgggacct
     9241 gtcctaggtc tagactataa cagagaagaa attccacctg tgaataggca taatactgtg
     9301 gaatatagag gatatttcct acaatgatca agatgagact tgcaagaaag tagagtccaa
     9361 agaccatgag gaaacgctcg gtttcaactg atgagagatc tagatttgga aactcaggat
     9421 gtagggtgac gaattttttg gctaaaaagc tactataaaa gaggaggtaa atcccaagta
     9481 aattagggat actccataaa aagagataga aacgtttgag aagtagggtc aaaaaggttt
     9541 gagaaaagcg ctcctcatca aagagagcta ggctgttttt tacagatggc tccgttttag
     9601 aatctttcat gagtgtcagt gttgcataga cggaactggt caaaagaata gtcccgataa
     9661 aggagactag tagaggaaag aggtaggttt gaagtatttg gccaagtatg ctgaaaaatg
     9721 gctgttctaa aacagtcccg tggatccgag ataagggatt aagaaaacca gataagatga
     9781 ccagcatact gggaaggata tagaggagaa agagacgggg ggtgtcagcc tgaaaatgtt
     9841 ttgactcctg acgaattgtt tttaaatcaa tttttggata gttcattctc ttattatacc
     9901 atagttctta tacatagttc gtgacagttc ctactttttt tgataaaatc atacagtgtg
     9961 tccttgggca cactgtatga actgggactg tctttcccag cttcggaggt aaaaaatgtc
    10021 agattcacca atcaaatatc gtttgattaa gaaagaaaaa cacacaggag ctcgtctggg
    10081 agaaatcatc actccccacg gtacctttcc gacacctatg tttatgccag ttgggacaca
    10141 agccactgtc aaaactcagt cacctgaaga attgaaggag atgggttcgg gaattatcct
    10201 atcaaacacc tatcatctct ggcttcgccc tggagatgaa ctcattgcac gcgctggtgg
    10261 tctccacaag ttcatgaatt gggaccagcc tatcttgaca gatagtggtg gttttcaggt
    10321 ttattcttta gcagatagcc gtaatatcac agaagaagga gtaaccttta aaaatcatct
    10381 aaatggttct aagatgttcc tatccccaga aaaagccatc tctattcaga ataatctggg
    10441 ttcagacatc atgatgtcct ttgatgaatg tcctcagttt tatcaacctt atgactacgt
    10501 taagaaatcg atcgagcgta ccagccgttg ggctgagcgt ggtttgaagg ctcaccgtcg
    10561 tccacatgac caaggtttgt ttggaattgt gcaaggtgca ggatttgaag accttcgccg
    10621 ccaatcagct catgatcttg tcagcatgga tttctcaggc tactctatcg gtggtttggc
    10681 agtgggagaa acccatgaag agatgaatgc ggtcttggac tttacaactc aactgctgcc
    10741 tgaaaataaa cctcgttatc tgatgggtgt gggagcgcca gatagcttga tcgatggggt
    10801 cattcgtggg gtggatatgt ttgactgtgt cttaccgact cgaattgctc gtaacgggac
    10861 ttgtatgacc agtcaaggac gtttggttgt gaaaaatgcc cagtttgctg aggactttac
    10921 gccactggat cctgagtgtg attgctacac atgtaataac tatacacgcg cttaccttcg
    10981 tcacctgctc aaggctgatg aaacctttgg tatccgcttg actagctacc acaatcttta
    11041 cttcttgctt aacctgatga agcaagtgcg acaagccatc atggatgaca atctcttgga
    11101 attccgtgag tattttgtgg aaaaatatgg ctataataag tcaggacgta atttctaaaa
    11161 tggaattgat ataaaaaaat cctaagtttt ctcttaggat ttttcttctt tttttgatag
    11221 aataaagtgt acaatgaaag gaagaataaa ctcgtatgcg cattaaatgg ttttccttga
    11281 ttaggattat aggtttactt ttggtactct tgtatcactt ctttcagacg atctttcctg
    11341 gaggattttt cggggtagat gtctttttca cattttcagg tttcctgatt acagctctac
    11401 tcattgaaga attttctaag aacaatgaga ttgatttgat aggatttttt agaagacgct
    11461 tttatcggat tgtgccacct gtggttttga tggtcttggt gaccatgcct tttactttct
    11521 tggttcgcca agactatgtt gctggaattg gtggccagat tgcgggcgtc ttaggcttta
    11581 tgaccaactt ctatgaactc ctaacaggtg ggagttatga atctcagttc attcctcatt
    11641 tgtttgttca taattggagt ttggcagttg aggttcacta ctatattctt tggggattgg
    11701 cagtttggtt cttatccaaa caagctaaat caaatggtca gttgaagggg atggtctttc
    11761 tcttatctgc tgttgccttc ttgatcagtt tcttctccat gtttattggt agttttctag
    11821 tgacctctta ttcctctgtt tatttctcca gtttaactca tgtctatcca ttctttttag
    11881 gaagtatgtt agcaactatt gtaggcgttc gtcagacgac ttccctcgtc aagcagttgg
    11941 ataaaatctg ggatttacga aagactttgg tagtttttgg aggaggcttt ggtttcctag
    12001 ttcttttgac tttctttgtc aaattcactt atctttttgc ctatcttatc ggcttcttac
    12061 ttgccagtct tgcagctctt gccatgattc tggcggcgcg tgtcttacat gaaaagacac
    12121 atcatataca ggagtcgaag attatcagct ttttagcgga tactagctat gcggtttatc
    12181 ttttccattg gcctttctat atcattttct cacagttgac atcaaatctt cttgctgtat
    12241 tactgactct gatttgttct tatggctttg ccagtctgtc attttatgta ttggaacctt
    12301 ggattgcagg caagaacaca cctattgtcc aaacccttcg tcccctgcct tatattcacg
    12361 caattcttgc agcaggtaca ggaatcttga ccatcattgt ctgcacggtg accttgttgg
    12421 caccacaagt gggagcgttt gagacagact tgactgtcaa tggcttgaag caagctgcaa
    12481 caaatattgg ccagaccaag gtgatggcag aacgggcaga tgcaaacagt ttgggaattg
    12541 ctgatggcac tatgttaatt ggtgactcag tggctttaag ggcaaataca gcactacaga
    12601 cagctcttcc tggagcacag attaacgcgc aggtcagcgt aacaaccaag accgcaaatg
    12661 aaatcatgct aaataatagc cagaataaat ttttacctaa gacggtggtc attgcgactg
    12721 gggtaaataa tcctgagaat tacaaggatg actgggacag tatcgtgaaa aatcttccta
    12781 agggacacca tatgattttg gtgactcctt atgagggaga taagacaaaa gagacctatg
    12841 ccatcgttga gaaggctgct gcctatatga gagaattggc agagaagaca ccttacatta
    12901 cgatagcaga ttggaatcaa gttgcgaaag agcatccaga aatttgggct ggaacagacc
    12961 aggttcattt cgggagtgag agtagcacta tcgaagcagg agcaaaattg tatgcagata
    13021 cgattgccac agctttgcag acagctcaag acaagccggt taaatcaaaa taacttgtat
    13081 taaaaaagag aagagttgga gaaatccagc tcttttaaaa tatctccaaa aaataggtct
    13141 ataccattta caaataaaaa agaaaggttt ataatgtaat tgacataata aattgtagaa
    13201 tcaatctttt aaggaggtta acattatgcc taactatatt aaagcggatc agtttttcta
    13261 cccacacgga gttcgtcgag gtggttactt ggaacttgtg gacggcaagt ttgggaaaca
    13321 tgtagagcag attcctgaag gggctgaggt gattgactat acaggttata gcattgcccc
    13381 aggtcttgtg gatactcata ttcatggata tgcaggtgta gatgtgatgg acaacaacat
    13441 tgaaggtaca ttgcatacta tgagtgaagg acttcttagt accggtgtta ccagtttctt
    13501 acccacaact ttaacagcca cttatgagca attgcttgca gtcactgaaa atcttggaaa
    13561 ccattataaa gaagcaacag gtgctaagat tcgtgggatt tattatgaag gtccatattt
    13621 cacagaaact tttaaggggg cacaaaatcc aacttatatg agagacccgg gtgttgagga
    13681 gtttcattct tggcaaaaag cggcaaatgg cttgcttaat aaaattgccc ttgcaccaga
    13741 acgtgatggg gtggaagact ttgttcgtac agttacgggc gaaggtgtga cggttgctct
    13801 tggacattca aacgcgactt ttgatgaagc caaaaaagca atcgatgctg gagcgagtgt
    13861 ttgggtgcat gcctacaatg gaatgcgtgg gttgactcac cgtgaattgg ggatggttgg
    13921 agccatgtac caattgccac atacctatgc agagttgatc tgtgatggtc accacgtaga
    13981 tccaaaggct tgcgaaattc ttatcaaaca aaaaggaaca gaaaacatcg ctcttatcac
    14041 agactgtatg acagctgggg gattggaaga cggagattat atgttgggag aattcccagt
    14101 tgtcgttgca aatggaactg cacgcctcaa atcgacaggt aacttggcag gttctatcct
    14161 caaactcaaa gatggtttga agaatgtggt cgaatgggga attgcgaatc cgcatgaagc
    14221 agtcatgatg gccagcttca acccagctaa atccgttcac atcgatgacg tctgtggcca
    14281 aatccgtgaa ggctacgacg ctgacttcat cgtattagat aaagatttgg aattggtagc
    14341 aacctaccta gatggcgtaa aacgttatca agcataagag agaaagcaga agttagagac
    14401 taacttctgc ttttttatgc agggtacttt actggtaaat aaaaagttaa aaaaatcaca
    14461 aaaaaagctt gaagaaacaa agaaaaaggc ttacaattga actataaata ttacaaataa
    14521 aaaaacggag gagtgcttta tgaaagccta tacttatgtt aaaccaggac ttgcttcttt
    14581 tgttgatgta gacaaaccag ttattcgcaa gccaacagac gctattgtgc gtattgtaaa
    14641 aaccactatt tgtggaacag acctccatat tatcaaaggg gatgttccta cttgccaaag
    14701 tggtaccatt cttggccacg aagggattgg gattgttgaa gaagttgggg aaggagtttc
    14761 caacttcaaa aaaggtgaca aggtcttgat ttcttgcgtc tgtgcctgtg gtaaatgcta
    14821 ctactgtaaa aaaggaattt atgctcactg tgaagacgaa gggggctgga ttttcggtca
    14881 cttgattgat ggtatgcagg ctgaatatct acgtgtccct catgcagata atactcttta
    14941 ccatactcca gaagacttgt cagatgaagc tttggttatg ctgtcagaca ttctgcctac
    15001 tggatatgaa attggtgtct taaaagggaa agtagaacct ggttgcagcg tagccattat
    15061 tggttcaggt ccagttggat tggctgctct tttaacagcc caattctatt caccagctaa
    15121 attgattatg gtagacctag acgataaccg cttggaaact gccctatcat tcggtgcgac
    15181 tcataaggtt aattcttcag accctgaaaa agccattaaa gaaatttatg atttgacaga
    15241 tggtcgtggt gtggatgtcg ctatcgaagc tgttggtatt cctgcaacat ttgatttctg
    15301 tcaaaagatt atcggtgtag acggaacggt tgccaactgt ggtgtgcatg gtaaaccagt
    15361 tgaattcgat ttagataaac tttggattcg caacatcaat gtaacaactg gtttggtatc
    15421 tacaaatacg actccacaat tgttgaaagc acttgaaagt cataagattg aaccggaaaa
    15481 attggtaact cactatttca aactcagtga aattgaaaaa gcctacgaag tcttcagtaa
    15541 ggcagcagac caccatgcca ttaaggtcat tatcgaaaac gatatctcag aagcctaagt
    15601 agtaaaaata tttttgtaca taagtaaata gaaattcagt catccatcag atggctggat
    15661 tttttatcaa aaaattaaga aatgagcata tttctttcct tgtctggcgg aattggttat
    15721 aatatacggt acaaaggaat gaatgaatat gtatcgtgtt atagaaatgt acggagattt
    15781 tgaaccgtgg tggttcttag aaggttggga agaagatatt gtagcaagta gaaaatttga
    15841 ccagtattat gatgctctca aatactacaa aacttgctgg tttagattgg aacaagaatc
    15901 gcctctttat aaaagtagaa gcgacttgat gaccattttt tgggacccgg aagaccaacg
    15961 ctggtgtgat gaatgtgatg agtatttaca acaataccat tctttggctc ttttgcagga
    16021 tgagcaggtt atcccagacg aaaaactacg ctcaggctat gaaaaacaaa ccagtcagga
    16081 aaggaatcgt tcttgccgta tgaaattaaa atagagaaaa gtaacttttt tggagttgct
    16141 ttttttattt ttctaactct ttgcgaatag tataggtgag gaggtaagta tggttcaaga
    16201 aattgcacaa gaaatcattc gttcagctcg gaaaaaaggg acgcaggata tctattttgt
    16261 ccctaagtta gacgcctatg agcttcatat gagggtagga gacgagcgct gtaaaattgg
    16321 tagctatgat tttgaaaagt ttgcagccgt tatcagtcac tttaagtttg tggcgggtat
    16381 gaatgtggga gaaaaaagac gtagtcaact gggttcctgt gattatgcct atgaccataa
    16441 gatagcgtct ctacgtttat ctactgtagg cgattatcgg gggcatgaga gtttggttat
    16501 ccgtttgttg cacgatgagg agcaggacct gcatttttgg tttcaggata ttgaagaatt
    16561 aggcaagcag tacaggcaac ggggactcta tctttttgct ggtccggttg ggagtggtaa
    16621 gacgaccttg atgcatgaat tgtccaagtc actctttaaa ggacagcaag ttatgtccat
    16681 cgaagatcct gtcgaaatca agcaggacga catgcttcag ttgcagttga acgaagcaat
    16741 cggcctaacc tatgaaaatc taatcaaact ttccttgcgt catcgaccag atctcttgat
    16801 tatcggagaa attcgtgaca gcgagacggc gcgtgcagtg gtcagagcta gtttgacagg
    16861 tgcgacagtc ttttcaacca ttcacgccaa gagtatccga ggtgtttatg agcgtctgct
    16921 ggagttgggt gtgagtgaag aagaattggc agttgttctg caaggagtct gctaccagag
    16981 attaatcggg ggaggaggaa tcgttgactt tgcaagcaga gattatcaag aacaccaagc
    17041 agccaagtgg aatgagcaaa ttgaccagct tcttaaagat ggacatatca caagtcttca
    17101 ggctgagacg gaaaaaatta gctacagcta agcaaaaaaa tatcatcacc ctatttaaca
    17161 atctcttttc tagcggtttt catctggtgg agactatctc ctttttagat aggagtgctt
    17221 tgttggacaa gcagtgtgtg acccagatgc gtgtgggctt gtctcagggg aaatcattct
    17281 cagaaatgat ggaaagtttg ggatgttcaa gtgctattgt cactcagtta tccctagctg
    17341 aagttcatgg caatctccac ctgagtttgg gaaagataga agaatatctg gacaatctgg
    17401 ctaaggtcaa gaaaaaattg attgaagtag cgacctatcc cttgattttg ctgggttttc
    17461 ttctcttaat tatgctgggg ctacggaatt acctgctccc acaactggat agtagcaata
    17521 ttgccaccca aattatcggt aatctgcccc aaatttttct aggcatggta gggcttgttt
    17581 ccgtgcttgc ccttttagca ctcacttttt ataaaagaag ttctaagatg agtgtctttt
    17641 ctatcttagc acgccttccc tttattggaa tctttgtgca gacctacttg acagcctatt
    17701 atgcacgtga atgggggaat atgatttcac agggaatgga gttgacgcag atttttcaaa
    17761 tgatgcagga acaaggttcc cagctcttta aagaagtcgg tcaagatctg gctcaaaccc
    17821 tgaaaaatgg ccgtgaattt tctcagacga taggaaccta tcctttcttt aggaaggaat
    17881 tgagtctcat catagagtat ggggaagtta agtccaagct gggtagtgag ttggaaatct
    17941 atgctgaaaa aacttgggaa gcctttttta cccgagtcaa ccgcaccatg aatttggtgc
    18001 agccactggt ttttatcttt gtggcactga ttatcgtttt actttatgcg gcaatgctca
    18061 tgcccatgta tcaaaatatg gaggtaaatt tttaaaatga aaaaaatgat gacattcttg
    18121 aaaaaagcta aggttaaagc ttttacattg gtggagatgt tggtggtctt gctgattatc
    18181 agcgtgcttt tcttgctctt tgtacctaat ctgaccaagc aaaaagaagc agtcaatgac
    18241 aaaggaaaag cagctgttgt taaggtggtg gaaagccagg cagaacttta tagcttagaa
    18301 aagaatgaag atgctagcct aagaaagtta caagcagatg gacgcatcac ggaagaacag
    18361 gctaaagctt ataaagaata caatgataaa aatggaggag caaatcgtaa agtcaatgat
    18421 taaggccttt accatgctgg aaagtctctt ggttttggga cttgtgagta tccttgcctt
    18481 gggcttgtcc ggctctgtcc agtccacttt ttcagcggta gaggaacaga ttttctttat
    18541 ggagtttgaa gaactctatc gggaaaccca aaaacgcagt gtagccagtc agcaaaagac
    18601 tagtctgaac ttagatgggc agacgcttag caatggcagt caaaagttgc cagtccctaa
    18661 aggaattcag gccccatcag gccaaagtat tacatttgac cgagctgggg gcaattcgtc
    18721 cctggctaag gttgaatttc agaccagtaa aggagcgatt cgctatcaat tatatctagg
    18781 aaatggaaaa attaaacgca ttaaggaaac aaaaaattag ggcagtgatt ttactggaag
    18841 cagtagtcgc tctagctatc tttgccagca ttgcgaccct ccttttggga caaattcaaa
    18901 aaaataggca agaggaagca aaaatcttgc aaaaggaaga agtcttgagg gtagctaaga
    18961 tggccctgca gacggggcaa aatcaggtaa gcatcaacgg agttgagatt caggtatttt
    19021 ctagtgaaaa aggattggag gtctaccatg gttcagaaca gttgttggca atcaaagagc
    19081 cataaggtca aggcttttac cttgttagaa tccctgcttg ccctcattgt catcagtggg
    19141 ggattactcc tttttcaagc tatgagtcag ctcctcattt cagaagttcg ctaccagcaa
    19201 caaagcgagc aaaaggagtg gctcttgttt gtggaccaac ttgaggtaga attagaccgt
    19261 tcgcagttcg aaaaagtaga aggcaatcgc ctatacatga agcaagatgg caaggacatc
    19321 gccatcggta agtcaaagtc agatgatttc cgtaaaacga atgctcgtgg tcgaggttat
    19381 cagcctatgg tttatggact caaatctgta cggattacag aggacaatca actggttcgc
    19441 tttcatttcc agttccaaaa aggcttagaa agggagttca tctatcgtgt ggaaaaagaa
    19501 aaaagttaag gcaggtgttc tcctctacgc agtcaccata gcagccatct ttagtctttt
    19561 gttgcaattt tatttgaacc gacaagtcgc ccactatcaa gactatgctt tgaataaaga
    19621 aaaattggtt gcttttgcta tggctaaacg aaccaaagat aaggttgagc aagaaagtgg
    19681 ggaacagttt tttaatctag gtcaggtaag ctatcaaaac aagaaaactg gcttagtgac
    19741 gagggttcgt acggataaga gccaatatga gtttctgttt ccttcagtca aaatcaaaga
    19801 agagaaaaga gataaaaagg aagaggtagc gaccgattca agcgaaaaag tggagaagaa
    19861 aaaatcagaa gagaagcctg aaaagaaaga gaattcatag tcaattcaac tataatgcgt
    19921 tgaatccaga atagtccact gtagtttcta gaaaattgct ggaaatggat gttaagctcc
    19981 aattcatttg tttatatctt atttcagttt actatacttt gtgctaaatt aaagatatga
    20041 aacatgattt taaccacaaa gcagaaactt tcgattcccc taaaaatatc ttcctcgcaa
    20101 acttggtatg tcaagcagcc gagaaacaga ttgatcttct atcagacaaa gaaattttag
    20161 atttcggtgg tggcacgggt ctattagcct tgcccctaac ccctagccaa gcaggctaag
    20221 tcagtcactc ttgtagacat ttctgagaaa atgttggagc aagctcgttt gaaagtggag
    20281 cagcaagcaa tcaagaatat ccagtttttg gagcaagatt taccgaaaaa tcccttggag
    20341 aaagagtttg attgccttgc tgttagtcgg gttcttcatc atatgcctga tttggatgcg
    20401 gctctctcac tgtttcatca acatttgaag gaagatggga aactcatcat tgctgatttt
    20461 accaagacag aagctaatca tcatggattt gatttagctg aactggaaaa caagctaatt
    20521 gagcatggtt tttcatctgt gcatagtcag attctctata gtgctgaaga cctgtttcaa
    20581 ggaaatcact cagaattctt tttaatagta gcccaaaaat cactcgccta gtcagggagt
    20641 gatttttcta taaggatgga aaaaagaagg gaaatttggt aagataggaa tatggatttt
    20701 gaaaaaattg aacaagctta tacctattta ctagagaatg tccaagtcat ccaaagtgat
    20761 ttggcgacca acttttatga cgccttggtg gagcaaaata gcatctatct ggatggtgaa
    20821 actgagctaa accaggtcaa ggagaacaat caaaccctta agcgtttagc actacgcaaa
    20881 gaagaatggc tcaagaccta ccagtttctc ttgatgaagg ctgggcaaac agaacccttg
    20941 caggccaatc accagtttac accggatgct attgctttgc ttttggtgtt tattgtggaa
    21001 gagttgttta aagaggagga aattactatc ctcgaaatgg gttctgggat gggaattcta
    21061 ggcgctattt tcttgacctc gcttactaaa aaggtggatt acttgggaat ggaagtggat
    21121 gatttgctga ttgatctggc agctagcatg gcagatgtaa ttggtttgca ggctggcttt
    21181 gtccaaggag atgccgttcg cccacaaatg ctcaaagaaa gcgatgtggt catcagtgac
    21241 ttgcctgtcg gctattatcc tgatgatgcc gttgcgtcgc gccatcaagt tgcttctagc
    21301 caagaacata cttacgccca tcacttgctc atggaacaag ggcttaagta cctcaagtca
    21361 gacggatacg ctatttttct agctccgagt gatttgttga ccagtcctca aagtgatttg
    21421 ttaaaagaat ggctgaaaga agaggcgagt ctggttgcta tgattagtct gcctgaaaat
    21481 ctctttgcta atgccaaaca atctaagact atttttatct tacagaagaa aaatgaaata
    21541 gcagtagagc cttttgttta tccacttgct agcttgcaag atgcaagtgt tttaatgaaa
    21601 tttaaagaaa attttcaaaa atggactcaa ggtactgaaa tataaaatag attttgttat
    21661 aatagttgaa aacgcttaaa aaggggtatc atgttatgac aaaaacaatt gcaatcaatg
    21721 caggaagttc aagtttgaaa tggcaattat acttaatgcc agaagaaaaa gtattggcga
    21781 aaggtttgat tgaacgtatc ggtttgaaag attcaatttc aactgtaaaa tttgacggcc
    21841 gttctgaaca acaaattttg gatattgaaa atcatataca agccgttaaa attttattgg
    21901 atgacttgat tcgtttcgat attatcaagg cttatgacga gattacaggt gttggacatc
    21961 gtgttgttgc tggtggagaa tatttcaaag aatcaacagt tgttgaggga gatgttttag
    22021 aaaaagttga agagttgagt ttgttggctc ctctacacaa cccggccaat gcagcaggtg
    22081 ttcgtgcctt caaggaattg ttgccagaca ttaccagtgt agttgttttt gatacttcct
    22141 tccacacaag tatgccagag aaagcttatc gctaccctct accaacaaaa tattacacag
    22201 aaaacaaggt tcgtaaatac ggtgctcatg gtacaagtca ccagtttgta gcaggagaag
    22261 ctgcaaaact cttgggacgt ccattagaag acttgaagtt aattacctgt catattggta
    22321 acggaggctc aattacagct gtgaaagccg gcaaatctgt agacacttct atggggttca
    22381 ctcctcttgg tggtattatg atgggaacgc gtacagggga tattgatcca gctatcattc
    22441 cttatttaat gcaatataca gaggatttta acacaccaga agatatcagt cgtgttctta
    22501 accgtgaatc aggtcttttg ggagtttctg ctaattctag cgatatgcgc gatatagaag
    22561 cagctgtagc agaagggaat cacgaggcta gcttggctta tgaaatgtat gttgaccgta
    22621 tccaaaaaca tatcggtcag taccttgcag tgctaaatgg agcagatgcc attgttttca
    22681 cagcaggtgt cggtgaaaat gcagagagtt tccgtcgtga tgtaatctca gggatttcgt
    22741 ggtttggttg tgatgttgat gatgaaaaga atgtctttgg cgttacagga gacatctcaa
    22801 cagaggcagc taaaatccgt gtcttggtta ttccaacaga tgaagaatta gtcattgccc
    22861 gtgacgttga acgcttgaaa aaataagtga aactaaaaaa atattcaata caaggagttg
    22921 ggaaagttat ttttccagct tctttttctg atgaaattgt ccaaaacctt gctatgattg
    22981 gcttttttga aaaatatggt ataatagtag taatttaata gatggagttg agttttgaag
    23041 aaaaactttc gtgtaaaaag agagaaagat tttaaggcga ttttcaagga ggggacaagt
    23101 tttgctaatc gcaaatttgt ggtctaccaa ttagaaaacc agaaaaaccg ttttcgagta
    23161 ggtctatcag ttagcaaaaa actggggaat gccgtcacta gaaatcaaat taagcgacgg
    23221 attcggcata ttatccagaa tgcaaaaggg agtctggtag aagatgtcga ctttgttgtc
    23281 attgctcgaa aaggagtcga aaccttggga tacgcagaga tggagaaaaa tctactccat
    23341 gtattaaaat tatcaaagat ttaccgggaa ggaaatggga gtgaaaaaga aactaaagtt
    23401 gactagtttg ctaggactgt ctctgttaat catgacagcc tgtgcgacta atggggtaac
    23461 tagcgatatt acagccgaat cggctgattt ttggagtaaa ttggtttact tctttgcgga
    23521 aatcattcgc tttttatcgt ttgatattag tatcggagtg gggattattc tctttacggt
    23581 cttgattcgt acagtcctct tgccagtctt tcaggtgcaa atggtggctt ctaggaaaat
    23641 gcaggaagct cagccacgca ttaaggcgct tcgagaacaa tatccaggtc gagatatgga
    23701 aagcagaacc aaactagagc aggaaatgcg taaagtattt aaagaaatgg gtgtcagaca
    23761 gtcagactct ctttggccga ttttgattca gatgccggtt attttggccc tgttccaagc
    23821 cctatcaaga gttgactttt taaagacagg tcatttctta tggattaacc ttggtagtgt
    23881 ggatacaacc cttgttcttc cgattttagc agcagtattc acctttttaa gtacttggtt
    23941 gtccaacaaa gctttgtctg agcgaaatgg cgctacgact gcgatgatgt atgggattcc
    24001 agtcttgatt tttatctttg cagtttatgc gccaggtgga gtcgccctat actggacagt
    24061 gtctaatgct tatcaagtct tgcaaaccta tttcttgaat aatccattca agattatcgc
    24121 agagcgcgag gccgtagtac aggcacaaaa agatttggaa aatagaaaaa gaaaagccaa
    24181 gaaaaaggct cagaaaacga aataaataag gaggaatctg gtagtggtag tatttacagg
    24241 ttcaactgtt gaagaagcaa tccagaaagg attgaaagaa ttagatattc caagaatgaa
    24301 ggctcatatc aaagtcattt ctagggagaa aaaaggcttt cttggtctat ttggtaaaaa
    24361 accagcccaa gtggatattg aagcgattag tgaaacgact gttgtcaaag caaatcaaca
    24421 ggtagtaaaa ggcgttccga aaaaaatcaa tgatttgaac gagcctgtga agacggttag
    24481 tgaagaaacc gttgaccttg gtcatgtggt tgatgctatt aaaaaaatag aggaagaagg
    24541 tcaaggtatt tctgatgaag tcaaggctga aatcttaaaa catgaaagac atgccagcac
    24601 tatcttagaa gaaactggtc acattgagat tttaaatgaa cttcaaatcg aggaagcgat
    24661 gagggaagaa gcaggcgctg atgaccttga aactgagcaa gaccaagctg aaagtcaaga
    24721 actagaagac ttgggcttga aagttgaaac gaactttgat attgaacaag tagctacgga
    24781 agtaatggct tatgttcaaa cgattattga tgacatggat gttgaggcta cactttcaaa
    24841 tgattataac cgtcgtagca tcaatctaca aattgacacc aacgaaccag gtcgtattat
    24901 cggctaccat ggtaaagtct tgaaggcctt gcaactgttg gctcaaaatt atctttacaa
    24961 ccgctattcc agaaccttct acgttacaat caatgtcaat gattatgtcg aacaccgtgc
    25021 agaagtcttg cagacctatg cgcaaaaatt ggcgactcgt gttttggaag aagggcgcag
    25081 tcataaaaca gatccaatgt caaatagcga acgcaagatt atccatcgta ttatttcacg
    25141 tatggatggc gtgactagtt actctgaagg tgatgagcca aatcgctatg ttgttgtaga
    25201 tacagaataa gtaaaatcag gtttatcctg attttttgct agttagagga ggttaaactg
    25261 atgttgaata agataagaga ctatttagac tttgctggtt tgcagtaccg taatcctgat
    25321 aaagcgggag cagagcgaga gaagatgctg gcattccgcc acaaaggaca agaggcccga
    25381 aaggttttta cagaactggc caaagccttt caagcaagcc atccagaatg gcaactccaa
    25441 cagactagcc agtggatgaa tcaggcccag cgtttgagac cacatttttg ggtttatcta
    25501 cagagagacg gacaagtgac agaacctatg atggccttac gtttgtatgg gacatctact
    25561 gactttggaa tttctttgga agtcagtttc atcgaacgta agaaggatga gcaaacactg
    25621 ggcaagcagg ccaaagtttt agacattcca accgttaaag ggatttatta tctaacctac
    25681 tctaatggtc aaagtcaacg gtgggaggcg aatgaagaaa agcgtcgtac tttacgcgag
    25741 aaggtgagaa gtcaagaagt tcgaaaagtt ttagtgaagg tagatgttcc tatgacagaa
    25801 aattcgtctg aagaagaaat cgtagaaggc ttattgaagt cttattctaa aattcttccc
    25861 tattatctag ctacgagaaa ataagataat ttgtaaaaca tcataaatca tacagtccaa
    25921 gagtgaacag tccgctgtgt aattcttggt ctttttgttt gcgctttcgc attatataat
    25981 aaacttacaa aaacaattca aaaggagaac aattatggaa gtcgtttcaa gtgttctaaa
    26041 ttggttttct agcaatattt tgcagaatcc cgcatttttc gtaggtttat tggtgttgat
    26101 aggatatgca cttttgaaaa aacctgccca tgacgttttt tcagggtttg ttaaagcaac
    26161 agtagggtat atgttgctta acgtgggtgc tggtggtttg gttacaacct ttcgtccaat
    26221 cttagcagct cttaactaca aattccaaat tggtgcagcg gttatcgacc cttactttgg
    26281 acttgctgca gcaaacaaca aaattgtagc agagtttcca gattttgttg gaactgcaac
    26341 tacagctcta ttgattggtt ttggaataaa tatcttgctc gtagctcttc gaaagattac
    26401 gaaggtaaga accctcttta ttactggtca catcatggta caacaagctg caacagtatc
    26461 tcttatggtt ctattcttag taccacaatt gcgcaatgct tacggtacag cagcgattgg
    26521 tatcatctgt ggactttact gggcagttag ttcaaatatg actgttgagg caactcaacg
    26581 cttgactggt ggtggcggat ttgcgattgg tcaccaacag caatttgcaa tctggtttgt
    26641 agataaagta gcaggacgct ttggtaagaa agaagaaagt ttagacaatc ttaaattacc
    26701 taagttcctc tcaatcttcc acgatacagt tgttgcatct gctaccttga tgctcgtatt
    26761 cttcggagcc attcttttaa tcttgggtcc agacattatg tctaataaag aagtcatcac
    26821 ttcaggaact ctattcaatc ctgctaaaca agatttcttt atgtacatta tccaaacagc
    26881 ctttaccttc tcagtttact tgttcgtttt gatgcaaggt gtccgaatgt tcgtatctga
    26941 gttgacaaac gccttccaag gtatttcaaa caaattgttg ccaggttcat tcccagcggt
    27001 tgacgttgca gcttcttatg gatttggttc tccaaatgct gtcttgtcag gatttacctt
    27061 tggtttgatt ggtcaattga ttacaattgt tttgctcatc gtctttaaaa atccgattct
    27121 tattattaca ggatttgtac cagtgttctt tgacaatgca gccattgcgg tctacgctga
    27181 taaacgcggc ggatggaaag cggctgttat cctttccttt atatcaggtg tccttcaagt
    27241 tgctctagga gctctttgtg tggcccttct cgatttggca tcttatggtg gctaccatgg
    27301 aaatatcgac tttgaattcc catggcttgg atttggatat atcttcaaat accttggtat
    27361 tgttggttat gtacttgtgt gtctcttctt gcttgttatt cctcaacttc aatttgccaa
    27421 agcaaaagat aaagagaaat attacaacgg tgaagttcaa gaagaagctt agtatctaga
    27481 aaaggagaaa taaaatggtt aaagtattag cagcgtgcgg aaatggaatg ggttcatcaa
    27541 tggttatcaa gatgaaggtt gaaaatgctc tccgtaagct taatcaaaca gattttacag
    27601 tcaattcatg cagtgtcggt gaagctaaag gtttagcagt aggatatgac atcgtaatcg
    27661 cttctcttca tttgattcaa gaattggaag ggcgaactaa tgggaagtta attgggcttg
    27721 ataacttgat ggatgataaa gaaatcaccg aaaaactcag tcaagcacta cagtaaaagg
    27781 ttggaggggg ctggacagaa actgagagtt atcgtttctg tccttctccc tctttaaata
    27841 aaggaggcag atatgaattt aaaacaagct ttaattgaca atgactcgat ccgactaggt
    27901 ttagaggcta acaattggaa agaagcagtc aaggtagcag tagatccctt aattgaaagt
    27961 ggggcaattt tgccagagta ttacgatgct atcattgaat cgactgaaga gtatgggcct
    28021 tactatatct tgatgccagg tatggctatg ccccacgcta gacctgaagc aggtgtgcaa
    28081 agtgatgcct tttcattgat taccttacaa aatcctgttg tattttcaga tgggaaagag
    28141 gtatctgttt tgttggcact agcagcaaca agttcaaaaa ttcacacaag tgtagccatt
    28201 ccacaaatta ttgccctatt tgaattagaa gattctattg cacgtttaca ggcttgccag
    28261 actaaagaag atgtcttggc tatgattgaa gaatctaagg atagccctta tctcgaagga
    28321 ttggatttgg aaagttagaa agaggaataa agaaatgaca aaaagaatac ctaatttaca
    28381 agttgcatta gaccattcag acttgcaagg agcgattaaa gcagctgttt ctgttggtca
    28441 ggaagtagat attatcgaag ctggaactgt ttgcttgctt caagttggaa gtgaactggc
    28501 tgaagtcttg cgtagccttt tcccagataa gattattgtg gcagacacaa aatgtgctga
    28561 tgctggtgga acagttgcta aaaataatgc ggttcgtgga gcagactgga tgacttgtat
    28621 ctgttgtgca accatcccta ctatggaagc agctctaaag gctatcaaga ctgaacgagg
    28681 agaacgaggc gaaatccaga tcgagcttta tggcgattgg acttttgaac aagctcagct
    28741 ttggctagat gcaggtatct cacaagctat ttatcaccaa tctcgtgatg ctcttcttgc
    28801 tggtgaaact tggggtgaaa aagaccttaa taaggttaaa aaactcattg acatgggctt
    28861 ccgtgtatct gtaacaggtg gtctagatgt agatactctc aaactctttg aaggtattga
    28921 tgtctttacc tttatcgcag gtcgtggaat tacagaggct gtggatccag caggagcagc
    28981 gcgtgccttc aaggatgaaa tcaaacgaat ttgggggtaa atcatggtac gtccaattgg
    29041 aatttatgaa aaggcaaccc caacacactg tacttggcta gaacgtttaa attttgccaa
    29101 ggagttaggc tttgattttg tcgagatgtc tattgacgaa cgtgacgagc gtttagcaag
    29161 acttgactgg agtaaggaag aacgcttgga agttgtcaaa gcaatctatg aaactggtgt
    29221 tcgtattcct tctatctgtt tttcaggcca tcgtcgctac ccattgggtt caaaagatcc
    29281 agttctagag gaaaaatctc tagaactcat gaaaaaatgt atcgaattag ctcaagactt
    29341 gggagttcgt acgattcaat tagctggtta cgatgtttac tatgaggaaa agtcacccca
    29401 gacacgccaa cgttttatca aaaatttgag aaaagcctgt gactgggctg aagaagctca
    29461 ggtggtactt gctattgaaa ttatggatga tcctttcatc agtagcatcg aaaaatattt
    29521 ggctatagaa aaagagattg actctccctt cctctttgta tatccagata ttggtaatgt
    29581 gtctgcatgg cataatgata tctatagtga gttttatctt ggtcatcatg ccatcgcagc
    29641 tctccatctc aaggatactt atgcagtgac agaaagttca aagggccagt tccgagatgt
    29701 acctttcggg caaggttgtg tcaaatggga agaagctttc gatattttaa aggaaaccaa
    29761 ttataatgga cctttcctaa tcgaaatgtg gtctgaaaat tgtgaaacag tagaagaaac
    29821 acgcgcagcc attcaagagg cgcaagcttt tctctatcca ctcattaaga aagcaggttt
    29881 gatgtaagat gaatcaagta atcaatgcta tgcgtaaacg agtctgtgat gccaatcaat
    29941 cattgccaaa acatggactt gtcaaattta cctgggggaa tgtatctgaa gttaatcgcg
    30001 aactcggtgt cattgttatc aaaccatcag gcgtggatta tgacgaattg acacctgaaa
    30061 acatggtagt gactgatcta gatggtaaga tcctagaagg ggatttaaga ccatcttccg
    30121 acctcccaac tcatgtgcaa ttatataaga cttggtcaga aattggtagt gtggttcaca
    30181 cccattcgac agaagctgtt ggttgggctc aggcaggtcg tgatattcct ttctacggaa
    30241 caacccatgc agattatttc tacggttcaa tcccttgcgc ccgtagtttg accaaggacg
    30301 aagtagaagt ggcctatgaa aaagatactg gcctggttat cgtagaagag tttgaacatc
    30361 gcggacttaa cccggttgaa gtaccaggaa ttgttgtacg caatcacggt ccattcacct
    30421 ggggcaaaaa tccagagaat gctgtttatc actctgtcgt actagaggaa gtatcaaaga
    30481 tgaatcgctt tacagaacaa atcaatccaa gagttggacc tgctccccag tacatactag
    30541 aaaaacacta ccaacgtaaa catggaccaa atgcttatta tggtcaaaag taagaacgat
    30601 gaaggaggag aaaaagataa atttagctcc tctttttaca tttgattttt attgagagta
    30661 aagttggagt tgaagtaatt ttaaaagatt ttttagaaat agcgcttgat atatatatgg
    30721 taaaataaaa agaattgctg tgatatcaat agatttgggg gattttttaa tatggtactg
    30781 gataaggcaa gttgtgattt gcttcaatat ttgatggatc aagaaacgtc caaaacgatt
    30841 atggcgattt cgaaagattt gaaagagtca agaaggaaaa tttattatca cattgacaaa
    30901 atcaatgctg ctctgggtga cgaggcgctt cacatcatta gtattccacg aattggtatt
    30961 cacttaacgg aagagcagag agatgcttgt tgtaaactat tatcggaagt agattcgtac
    31021 gattatatca tgagtgcgca tgaacgtatg atgataatgt tactatggat aggtatttct
    31081 aaagaacgta ttacgattga aaaattgata gagttaacag aggtatctag gaatactgtt
    31141 ctcaatgatt tgaatagtat tcgttatcaa ctaactttgg aacaatatca ggtgatcttg
    31201 caagtgagca agtcacaggg atacaacctt catgcccacc ctcttaataa aattcagtat
    31261 cttcaatcgc ttctatatca tatttttatg gaagaaaatg ccacttttgt atctatttta
    31321 gaagataaga tgaaagagag gttagatgat gagtgtttgc tttctgttga aatgaaccaa
    31381 ttttttaagg aacaggttcc tttagttgaa caagatttag ggaagaaaat aaaccatcat
    31441 gaaataactt ttatgttgca ggttctacct tatttgctgt taagctgtca taatgttgaa
    31501 cagtatcaag aaagacatca ggatatagag aaagaatttt ctttgataag aaaaagaata
    31561 gagtatcagg tgtctaagaa attaggagaa cggttgtttc aaaagtttga aatttctttg
    31621 tcaggacttg aagtttctct tgtagctgtt ctcctcctct cctatcgtaa agatttggat
    31681 attcatgcag aaagtgatga ttttcggcaa ttaaaacttg ctttagaaga atttatctgg
    31741 tattttgaat cacaaatccg aatggagatt gagaacaagg atgatttgtt acgaaatttg
    31801 atgatccact gtaaagcctt gttatttaga aagacttacg gtattttttc taaaaatcct
    31861 ctaacaaaac aaattcgatc caagtatgga gaattatttt tagtcactag aaaatctgcg
    31921 gaaattttag aaggagcatg gtttattcgg ctaacagacg atgatattgc ctatttgacg
    31981 attcatattg gaggattttt aaaatataca ccatcatctc aaaaaaatat gaaaaaagtt
    32041 tatctcgttt gtgatgaagg tgttgcggtt tcgagacttt tgctgaaaca atgcaaactt
    32101 tattttccaa atgagcaaat tgacactgta tttacaacag aacaatttaa gagtgtggaa
    32161 gatattgcac aagttgatgt agtgattact actaatgatg atttggatag cagatttccg
    32221 attttaaggg ttaatcctat ccttgaagca gaagatattt tgaaaatgct agactatctt
    32281 aaacacaata tatttcgtaa taagagcaaa agtttcagtg aaaatctttc tagtcttatt
    32341 tcgtcttata ttgtagacag caagttggct agtaagttcc aagaagaggt tcaaacactt
    32401 ataaatcaag aaatagtagt tcaagctttt ttggaagata tttgaaggac agtccaatga
    32461 tgaacacaaa cctgtgtttt tcttggtctt ttttagtgtt ttgaagggtg gtatactaat
    32521 ctcaaagata acaattatat ccaaaggagg caacatatgc caaacgtcaa agaaattaca
    32581 agagagtcat ggattttagc cactttccca gagtggggaa catggttgaa cgaagaaatc
    32641 gaagaagaag tcgtacctga aggcaacttt gccatgtggt ggctaggcaa ctgtggtact
    32701 tggattaaga caccagctgg tgctaacgtt gtcatggacc tttggtcaaa ccgtggaaaa
    32761 tcaaccaaaa aagtgaaaga tatggttcgt gggcaccaaa tggcaaatat ggcaggtgtt
    32821 cgtaagctgc aaccaaactt gcgtgttcag ccaatggtta tcgatccatt tgctatcaac
    32881 gaactagact attacttagt ttcacacttc cacagtgatc atatcgaccc atacacagct
    32941 gcagcaattc tcaataatcc taagttagag catgttaagt ttatcggtcc ttaccactgt
    33001 ggacgaatct gggaaggatg gggtgttcca aaagaacgta tcatcgttgt taaaccaggt
    33061 gacactatcg aattaaaaga tatgaagatt catgcagtag aatcatttga ccgtacttgc
    33121 ttggtaactc tcccagtgaa cggtgctgat gagacaggcg gtgaacttgc tggcttggct
    33181 gttacagatg aagaaatggc tcaaaaggct gttaactata tctttgaaac accaggtgga
    33241 accatctatc atggtgcaga ttctcacttc tcaaactatt ttgcaaaaca tggtaaagac
    33301 tttaaaattg atgttgcttt gaataactat ggtgaaaatc cggtaggtat ccaagacaaa
    33361 atgacatcta tcgaccttct tcgtatggca gaaaatctgc gtaccaaagt cattatccca
    33421 gttcactatg atatctggtc taacttcatg gcttctacta atgagattct agaactttgg
    33481 aaaatgcgaa aagatcgctt gcaatacgat ttccatccat ttatctggga agttggcggt
    33541 aagtacactt atcctcaaga tcaacactta gtagaatacc atcatccacg tggttttgat
    33601 gattgttttg aacaagactc taacattcaa tttaaagctt tgctataata taaaaatatt
    33661 ctttggaggt tatccgtatg ttgcatgata cggataattt tcatataata gtaaaataga
    33721 atgtgtgatt caataatcac ctcaaataga aaggaaattc tatgtcaaat ctatctgtta
    33781 atgcaattcg ttttctaggt attgacgcca ttaataaagc caactcaggt catccaggtg
    33841 tggttatggg agcggctccg atggcttaca gcctctttac aaaacaactt catatcaatc
    33901 cagctcaacc aaactggatt aaccgcgacc gctttattct ttcagcaggt catggttcaa
    33961 tgctccttta tgctcttctt cacctttctg gttttgaaga tgtcagcatg gatgagatta
    34021 agagtttccg tcaatggggt tcaaaaacac caggtcaccc agaatttggt catacggcag
    34081 ggattgatgc tacgacaggt cctctagggc aagggatttc aactgctact ggttttgccc
    34141 aagcagaacg tttcttggca gccaaatata accgtgaagg ttacaatatc tttgaccact
    34201 atacttacgt tatctgtgga gacggagact tgatggaagg tgtctcaagc gaggcagctt
    34261 catacgcagg cttgcaaaaa cttgataagt tggttgttct ttatgattca aatgatatca
    34321 acttggatgg tgagacaaag gattccttta cagaaagtgt tcgtgaccgt tacaatgcct
    34381 acggttggca tactgccttg gttgaaaatg gaacagactt ggaagccatc catgctgcta
    34441 tcgaaacagc aaaagcttca ggcaagccat ctttgattga agtgaagacg gttattggat
    34501 acggttctcc aaacaaacaa ggaactaatg ctgtacacgg cgcccctctt ggagcagatg
    34561 aaactgcatc aactcgtcaa gccctcggtt gggactacga accatttgaa attccagaac
    34621 aagtatatgc tgatttcaaa gaacatgttg cagaccgtgg cgcatcagct tatcaagctt
    34681 ggactaaatt agttgcagat tataaagaag ctcatccaga actggctgca gaagtagaag
    34741 ccatcatcga cggacgtgat ccagtcgaag tgactccagc agacttccca gctttagaaa
    34801 atggtttttc tcaagcaact cgtaattcaa gtcaagatgc cttgaatgtt gtggcagcta
    34861 aattaccaac ctttctaggt ggatcagctg accttgctca ctcaaacatg acttatatca
    34921 agacggatgg acttcaagac gatgccaatc gcttgaatcg taacattcag tttggtgttc
    34981 gtgaatttgc aatgggaaca atcttgaacg ggatggccct tcatggtgga cttcgtgtat
    35041 acggtggaac tttcttcgtc ttctctgact atgtgaaggc agctgtccgc ttgtcagcct
    35101 tacaaggact tcctgtgact tatgtcttta cccatgattc aatcgcagtt ggggaagatg
    35161 gtccgactca tgaaccagtt gagcatttag caggtcttcg tgctatgcca aatctaaatg
    35221 ttttccgtcc agcagatgcg cgtgaaacgc aagcagcttg gtaccttgca gtgacaagtg
    35281 agaaaacacc aactgccctt gtcttgacac gtcaaaattt gactgttgaa gatggaacag
    35341 acttcgacaa ggttgctaaa ggtgcttatg ttgtatatga aaatgcagcc gactttgata
    35401 ccatcttgat tgcgacaggt tcagaggtta atcttgctgt ctcagctgct aaagaattgg
    35461 ctagtcaagg cgaaaaaatc cgcgtagtca gcatgccatc tacagatgtc tttgataaac
    35521 aagatgcagc ttacaaggaa gaaattcttc caaatgcagt ccgccgtcgt gttgcagtcg
    35581 aaatgggtgc aagtcaaaac tggtacaaat atgttggtct cgatggtgcc gttctaggta
    35641 ttgatacctt cggagcctct gccccagcac caaaagtatt ggcagaatat ggctttactg
    35701 tagaaaatct tgtaaaagtt gttcgaaact tgaaataatc ctaaaaatca gggcgtaagc
    35761 tctggttttt cttaccagaa aagtaaggta caatcttgta aaagtagctg aaatttgata
    35821 tagtagtcct atgtaaaaga caaaggagaa aacaatgaat ccaaatatta cttttttaat
    35881 catgcttgta ggtatgatgg ccttgatgtt ctttatgcaa cgttctcaaa agaaacaagc
    35941 tcaaaagcgt atggaaagct taaataaact acaaaaaggc tatgaagtga ttacaatcgg
    36001 tggactttat ggaacagtcg atgaagtaga tacggagaag ggaacaatag ttcttgacgt
    36061 agatggagtt tacttgactt ttgaactagc tgctatcaag acagtattac cgctgaaaga
    36121 aacagcttca ctagaaggcg caattgaaaa ataagacggg atcacgaact cccgttttct
    36181 ataaaagaaa ggaaatggga tgaaaaaatt agtctttgtc tgtctgggaa atatttgccg
    36241 tagccctatg gccgagtttg ttatgaaatc aatgacagat aactacgaaa tccaaagtcg
    36301 agcaacttcc tcttgggaac atggcaatcc gattcataag gggactcagg gaatttttca
    36361 agagtatgag attccttatg acaagaacaa gacatcgctt cagattagta aggaagattt
    36421 tgaagccttt gattatatta tcggaatgga cgcttcaaat atttctgact tacgtcagat
    36481 gtgtccagta gactgtcaag ataagattta ctcattttca tctgaaagtg ttccagaccc
    36541 ttggtataca ggagattttg aagaaaccta tcgacgtgtt caagagggct gtcaagcttg
    36601 gttagaacgt ttagaaaagg agagttaagg tggaaaatct tacgaatttt tacgaaaagt
    36661 atcgtgtcta tctgactcgt ccacgtttag agcttttggc agtagttacc attgttttct
    36721 gtgctgtact cgtctttttt ctaaatattc caggaaaagg tgtcttaaaa ctcgataatg
    36781 gaacgattgt ttatgatggc agtcttgtcc gcggtaaaat gaatggccaa ggtaccatta
    36841 ccttccaaaa tggagaccaa tatacaggtg gcttcaacaa tggagccttc aacggaaaag
    36901 gtacctttca atctaaagaa ggctggacct acgaaggtga ttttgtaaat ggtcaggctg
    36961 aaggaaaagg gaaactaaca acagaacaag aagtcgttta tgaaggaact tttaaacaag
    37021 gcgtttttca acaaaaataa agcctcctta tcaaaggagg tattattaga atcgcaaggt
    37081 aagcgcttac ctgcaaatcc ctttctttcc aaatccctct tccaagcaag tttgtgaaat
    37141 aaaaaatatt tgaaataaat ttcacaaact tcaaagataa aacctgataa gaaaagaaaa
    37201 tgagaaaagt ttcacaagag tttaaaaatt ctttgtgaaa tgtttatgaa actgtttaca
    37261 aagttgaaat aatgcgttat aataaacttg tgaaaaaatt aacaaaggat atcgttcctt
    37321 gaaagctatg gaggaaaata tggctgataa aaaaactgtg acaccagagg aaaagaaact
    37381 cgttgctgaa aaacacgtag atgagttggt tcaaaaagct ctagttgccc ttgaagaaat
    37441 gcgtaaattg gatcaagaac aagttgacta catcgttgcc aaagcatcag tagcagcttt
    37501 ggatgcccac ggagaattgg ctttacatgc ctttgaagaa acaggacgtg gtgtatttga
    37561 agacaaagca actaagaact tgtttgcctg tgaacacgta gtaaacaaca tgcgccacac
    37621 taagacagtt ggcgttatcg aagaagacga tgtaacagga ttgactctta ttgctgaacc
    37681 agttggtgtt gtttgtggta ttactccaac aacaaaccca acatcaacag caatcttcaa
    37741 atcattgatt tcattgaaga cacgtaaccc aatcgtcttt gccttccatc catcagcaca
    37801 agaatcatct gctcatgcag ctcgtatcgt ccgcgatgca gctatcgcag ctggtgctcc
    37861 tgaaaactgt gtgcaatgga ttactcaacc atctatggaa gcaacaagtg cccttatgaa
    37921 ccacgaaggt gttgcgacaa tccttgcaac aggtggtaat gccatggtta aggcggctta
    37981 ttcatgtggt aaaccagctc ttggggtagg tgccggaaac gttccagctt atgttgaaaa
    38041 atcagcaaac attcgtcaag cagcacacga tatcgtcatg tctaaatcat ttgataacgg
    38101 tatggtctgt gcatctgaac aagcagttat cattgataaa gaaatttacg atgaatttgt
    38161 agcagagttc aaatcttacc acacttactt tgtaaacaaa aaagaaaaag ctcttcttga
    38221 agagttctgc ttcggcgtca aagcaaacag caaaaactgt gctggtgcaa aattgaacgc
    38281 tgacatcgtt ggtaaaccag caacttggat tgcagaacaa gcaggattta cagttccaga
    38341 aggaacaaac attcttgctg cagaatgtaa agaagttggc gaaaatgagc cattgactcg
    38401 tgaaaaattg tcaccagtta ttgcagtttt gaaatctgaa agccgtgaag atggtattac
    38461 taaggctcgt caaatggttg aatttaacgg tcttggacac tcagcagcta tccacacagc
    38521 tgacgaagaa ttgactaaag aatttggtaa agctgttaaa gctattcgtg ttatctgtaa
    38581 ctcaccttct acttttggtg gtatcgggga cgtttacaat gccttcttgc catcattgac
    38641 acttggatgt ggttcttacg gacgcaactc agttggggat aacgttagtg ccattaacct
    38701 cttgaatatc aaaaaagtcg gaagacggag aaataacatg caatggatga aacttccttc
    38761 aaaaacatac tttgaacgtg attcaattca ataccttcaa aaatgtcgtg acgttgaacg
    38821 tgtcatgatc gttactgacc atgccatggt agagcttggt ttccttgatc gtatcatcga
    38881 acaactggac cttcgtcgca ataaggttgt ttaccaaatc tttgcggatg tagaaccgga
    38941 tccagatatc acaactgtaa accgtggtac tgagattatg cgtgccttca aaccagatac
    39001 catcatcgca ctcggtggtg ggtctccaat ggatgctgcc aaagtaatgt ggctcttcta
    39061 cgagcaacca gaagtggact tccgtgacct tgtccaaaaa ttcatggata tccgtaaacg
    39121 tgccttcaag ttcccattgc ttggtaagaa gactaaattc atcgcgattc caactacatc
    39181 tggtacagga tctgaagtaa caccatttgc cgttatctct gataaagcaa acaaccgtaa
    39241 atacccaatc gctgactact cattgacacc aactgtggca atcgtagatc ctgctttggt
    39301 attgacagtt ccaggatttg ttgctgctga tactggtatg gacgtattga ctcacgcgac
    39361 agaagcatac gtatcacaaa tggctagtga ctacactgat ggtttagcac ttcaagccat
    39421 taaattggtc tttgaaaatc tcgaaagctc agttaagaat gcagacttcc actcacgtga
    39481 gaaaatgcat aacgcttcaa caatcgctgg tatggccttt gccaatgcct tcctaggtat
    39541 ttctcactca atggcccata agattggtgc gcaattccac acaatccacg gtcgtacaaa
    39601 tgctatcttg cttccatacg ttatccgtta caacggtaca cgtccagcta agacagcaac
    39661 atggcctaag tacaactact accgtgcaga tgaaaaatac caagatatcg cacgcatgct
    39721 tggacttcca gcttctactc cagaagaagg ggttgaatct tacgcaaaag ctgtctacga
    39781 actcggtgaa cgtattggga tccaaatgaa ttttagagac caaggaattg acgaaaaaga
    39841 atggaaagaa cattctcgta aattagcctt cctggcttat gaagaccaat gttcaccagc
    39901 taacccacgt cttccaatgg tagaccatat gcaagaaatc atcgaagatg catactatgg
    39961 ctacaaagaa agaccaggac gccgtaaata attgtttatc agtctagaag caagacaaaa
    40021 actcaatttg agggaaagat ccagtaattt ttctatgata aaaggcatcc tatcaaggtt
    40081 tttgaacacc tgataggatg cctttttatg atattgaggc ctttttgccc tttttgaaaa
    40141 actagaatag aaacaaaata tataatagat tgaaactaga atagtacata tctgcttcta
    40201 aaacattgtt agaattcgat ttgactgtcc tgatcgattt gtcctgttct tatttcattt
    40261 tgatatataa aaa
//
LOCUS       DAVKNL010000016        36328 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVKNL010000016 DAVKNL010000000
VERSION     DAVKNL010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36328
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.016"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            83..880
                     /locus_tag="VW411_000786"
     CDS             83..880
                     /locus_tag="VW411_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEU9793549.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            1028..1150
                     /locus_tag="VW411_000787"
     CDS             1028..1150
                     /locus_tag="VW411_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine sulfhydrylase"
                     /protein_id="HEU9793550.1"
                     /translation="MTCDFKFETLQLHAGQVVAPATKSRAVPIYQTTFFVFDDT"
     gene            1172..2212
                     /locus_tag="VW411_000788"
     CDS             1172..2212
                     /locus_tag="VW411_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HEU9793551.1"
                     /translation="MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILAIAH
                     AGDHVVAASTIYGGTFNLLKEPLPRYGITTTFFDIDNLEEVEAAIKDNTKLVLIETLG
                     NPLINIPDLEKLAEIAHKHQIPLVSDNTFATPYLINVFSHGVDIAIHSVTKFIGGHGT
                     TIGGIIVDSGRFDWTASGKFPQFVDEGPSCHNLSYTRDVGAAAFIIAVRVQLLRDTGA
                     ALSPFNAFLLLQRLETSSLRVERHVQNAETIVDFLVNHPKVEKVNYPKLADSPYHALA
                     EKYLPKGVGSIFTFHVKGGEEEARKVIDNLEIFSDLANAADAKSLVVHPATTTHGQLS
                     EKDLEAAGVTPN"
     gene            2383..3657
                     /locus_tag="VW411_000789"
     CDS             2383..3657
                     /locus_tag="VW411_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEU9793552.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMKQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHEALLAKDKEV
                     QLLENQLATLRLEHENQLQKTLSDLEKERDQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3667..4545
                     /gene="truB"
                     /locus_tag="VW411_000790"
     CDS             3667..4545
                     /gene="truB"
                     /locus_tag="VW411_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEU9793553.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTKTEDASGEVVAETPVLSLLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLSPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            4786..5088
                     /locus_tag="VW411_000791"
     CDS             4786..5088
                     /locus_tag="VW411_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU9793554.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQS
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            5196..5834
                     /gene="udk"
                     /locus_tag="VW411_000792"
     CDS             5196..5834
                     /gene="udk"
                     /locus_tag="VW411_000792"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEU9793555.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            5962..7302
                     /locus_tag="VW411_000793"
     CDS             5962..7302
                     /locus_tag="VW411_000793"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEU9793556.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMVTAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            7280..7492
                     /locus_tag="VW411_000794"
     CDS             7280..7492
                     /locus_tag="VW411_000794"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEU9793557.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            7489..8364
                     /locus_tag="VW411_000795"
     CDS             7489..8364
                     /locus_tag="VW411_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU9793558.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDSYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            8357..9172
                     /locus_tag="VW411_000796"
     CDS             8357..9172
                     /locus_tag="VW411_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEU9793559.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKVHQNVLESVTAMAVEV
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            9165..9596
                     /locus_tag="VW411_000797"
     CDS             9165..9596
                     /locus_tag="VW411_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9793560.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            9603..11270
                     /gene="recN"
                     /locus_tag="VW411_000798"
     CDS             9603..11270
                     /gene="recN"
                     /locus_tag="VW411_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEU9793561.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSYLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNKMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            11272..12000
                     /locus_tag="VW411_000799"
     CDS             11272..12000
                     /locus_tag="VW411_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEU9793562.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQPQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYSRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEAADLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            12079..13902
                     /gene="lepA"
                     /locus_tag="VW411_000800"
     CDS             12079..13902
                     /gene="lepA"
                     /locus_tag="VW411_000800"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEU9793563.1"
                     /translation="MNLEELKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMELERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(14106..14186)
                     /locus_tag="VW411_000801"
                     /pseudo
     CDS             complement(14106..14186)
                     /locus_tag="VW411_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            14360..14835
                     /locus_tag="VW411_000802"
                     /pseudo
     CDS             14360..14835
                     /locus_tag="VW411_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
     gene            14873..15175
                     /locus_tag="VW411_000803"
     CDS             14873..15175
                     /locus_tag="VW411_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9793564.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSVPVFDNIRLPAKQNMV"
     gene            15183..15988
                     /locus_tag="VW411_000804"
                     /pseudo
     CDS             15183..15988
                     /locus_tag="VW411_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            16037..16501
                     /locus_tag="VW411_000805"
     CDS             16037..16501
                     /locus_tag="VW411_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEU9793565.1"
                     /translation="MGGHPGFNCPLLDDEVYEDYYLEFEKEEICSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSTDAVTLDELQSRTIALRSLKHDKGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTSLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            16658..17083
                     /gene="lacA"
                     /locus_tag="VW411_000806"
     CDS             16658..17083
                     /gene="lacA"
                     /locus_tag="VW411_000806"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEU9793566.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFIVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            17114..17629
                     /gene="lacB"
                     /locus_tag="VW411_000807"
     CDS             17114..17629
                     /gene="lacB"
                     /locus_tag="VW411_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEU9793567.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPTEENKKLIAKIEHVESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            17640..18569
                     /locus_tag="VW411_000808"
     CDS             17640..18569
                     /locus_tag="VW411_000808"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEU9793568.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIKGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQEFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKHVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQALYDQLIVKEV"
     gene            18571..19551
                     /gene="lacD"
                     /locus_tag="VW411_000809"
     CDS             18571..19551
                     /gene="lacD"
                     /locus_tag="VW411_000809"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEU9793569.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMVKHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQRTATSWKERV"
     gene            19854..20426
                     /locus_tag="VW411_000810"
     CDS             19854..20426
                     /locus_tag="VW411_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793570.1"
                     /translation="MVSIFLSALPMVSGSVFADSALTTVDKANDIVLNVDGNKFYNVS
                     VSEDIVNAGQILEDYFYVDKFGNINLKGTPEELAKNIGISVQEASLMYGAVKELPNVY
                     ERGPVGFRFNLGPQVRGMGGWAAGAFATGYAGWHLKQFAVNPVTSGFVAVISGAIGWA
                     VKTAVENYWTVAVATVEVPFVNLVYTIDLP"
     gene            20824..21660
                     /locus_tag="VW411_000811"
     CDS             20824..21660
                     /locus_tag="VW411_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9793571.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            21696..22013
                     /locus_tag="VW411_000812"
     CDS             21696..22013
                     /locus_tag="VW411_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9793572.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVQ"
     gene            22013..23704
                     /locus_tag="VW411_000813"
     CDS             22013..23704
                     /locus_tag="VW411_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9793573.1"
                     /translation="MNKLIAFIEKGKPFFEKLSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQ
                     INYMSTLLAAIVGLLMLAADPIESGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
                     TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARYFVGSSVAESIGKFFAPLFSA
                     ADGYLGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLV
                     LNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFI
                     LAALLIVVDVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAG
                     VDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLPE
                     FDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQAQFD"
     gene            23913..25319
                     /gene="lacG"
                     /locus_tag="VW411_000814"
     CDS             23913..25319
                     /gene="lacG"
                     /locus_tag="VW411_000814"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEU9793574.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILLVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            25409..25621
                     /locus_tag="VW411_000815"
     CDS             25409..25621
                     /locus_tag="VW411_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEU9793575.1"
                     /translation="MNFEKCSQIPCLTSEELKSLGKWYVSTGKEWICHSDDELEEFKN
                     LFLNFINPEEWDTISFDSDFMPFQQS"
     gene            complement(25900..26661)
                     /locus_tag="VW411_000816"
     CDS             complement(25900..26661)
                     /locus_tag="VW411_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9793576.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNVSNLDTIVSDSKLSDSILFKLSKHIKVIKP"
     gene            complement(26949..27911)
                     /gene="nrdF"
                     /locus_tag="VW411_000817"
     CDS             complement(26949..27911)
                     /gene="nrdF"
                     /locus_tag="VW411_000817"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEU9793577.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLH"
     gene            complement(28099..30258)
                     /gene="nrdE"
                     /locus_tag="VW411_000818"
     CDS             complement(28099..30258)
                     /gene="nrdE"
                     /locus_tag="VW411_000818"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEU9793578.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(30339..30557)
                     /gene="nrdH"
                     /locus_tag="VW411_000819"
     CDS             complement(30339..30557)
                     /gene="nrdH"
                     /locus_tag="VW411_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEU9793579.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            30908..31171
                     /locus_tag="VW411_000820"
     CDS             30908..31171
                     /locus_tag="VW411_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEU9793580.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            31177..32910
                     /gene="ptsP"
                     /locus_tag="VW411_000821"
     CDS             31177..32910
                     /gene="ptsP"
                     /locus_tag="VW411_000821"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEU9793581.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            33164..35614
                     /gene="phtA"
                     /locus_tag="VW411_000822"
     CDS             33164..35614
                     /gene="phtA"
                     /locus_tag="VW411_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HEU9793582.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGDHYHF
                     IPYSQMSELEERIARIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPGPQPAPNLKIDSN
                     SSLVSQLVRKVGEGYVFEEKGISRYVFAKDLPSETVKNLESKLSKQESVSHTLTAKKE
                     NVAPRDQEFYDKAYNLLTEAHKALFENKGRNSDFQALDKLLERLNDESTNKEKLVDDL
                     LAFLAPITHPERLGKPNSQIEYTEDEVRIAQLADKYTTSDGYIFDEHDIISDEGDAYV
                     TPHMGHSHWIGKDSLSDKEKVAAQAYTKEKGILPPSPDADVKANPTGDSAAAIYNRVK
                     GEKRIPLVRLPYMVEHTVEVKNGNLIIPHKDHYHNIKFAWFDDHTYKAPNGYTLEDLF
                     ATIKYYVEHPDERPHSNDGWGNASEHVLGKKDHSEDPNKNFKADEEPVEETPAEPEVP
                     QVETEKVEAQLKEAEVLLAKVTDSSLKANATETLAGLRNNLTLQIMDNNSIMAEAEKL
                     LALLKGSNPSSVSKEKIN"
     gene            35772..>36328
                     /locus_tag="VW411_000823"
     CDS             35772..>36328
                     /locus_tag="VW411_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU9793583.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRYQAGQDKKESNRVAYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVNGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDG"
BASE COUNT        11264 a         6507 c         7940 g        10617 t
ORIGIN      
        1 acttttatga aatacagaaa acgcttccta aaacctaaag tttgtgatat aattatcaag
       61 aaaaaagatt taggagttta ttatggtttc ttcggaattt atctcaaaga ttgaatttgc
      121 ttgcaataag aaagaaagtc tttatagtca aagcaaattt aagtatgcga ttcgttcgat
      181 gttcgcaggt gcatttttaa ccttcagtac tgctgcaggt gcagttgggg ctgacttgat
      241 taataaaatt gcaccaggta gtggacgctt cctctttcca ttcgtttttg cttggggctt
      301 ggcctacatt gtttttttga atgccgagtt ggtcacttca aacatgatgt tcttgactgc
      361 tggtagtttc ttaaaaaaaa tctcttggag aaaaacagct gagattttac tatactgtac
      421 cttgttcaac cttatcggag ccttgatagc agggtggggc tttgctcatt cggcagccta
      481 tgcgaatctg acacacgata gtttcatctc aggtgttgtt gagatgaagt taggccgctc
      541 aaatgaattg gtcttgcttg aggcgatttt ggcaaatatt tttgtaaata ttgcgattct
      601 gtcatttatt ttggtcaaag atggtggtgc caaactttgg cttgtgttgt cagctattta
      661 catgtttgta ttcttaacaa acgagcacat tgcggcgaac tttgcttctt tcgcgattgt
      721 gaaattcagt gttgctgcgg attcaattgc caacttcggt gttggaaata tgcttcgcca
      781 ctggggtgtg actttcatcg gaaactttat cggaggaggc ctcttgatgg gtcttccata
      841 tgccttcctc aataaaaacg aagatactta tgtagattaa gaaaatgagc acgattgagt
      901 cgtgcttttt tcattttcaa aataaggtaa tagctatttc ttatatcaaa atatagaaaa
      961 ctgatatttg taaactataa ctcaaggtgc tacaatatcc ttaataaaat aatatggagg
     1021 tcaccttatg acttgtgatt ttaaatttga aactctacaa ctacatgctg gtcaagttgt
     1081 ggctccagct actaagtctc gtgcagtgcc gatttatcaa acaacatttt ttgtttttga
     1141 tgacacgtag gaaggtgccg atctgtttgc cttgaggaaa ccagggaaca tttatactcg
     1201 tatcaccaat cctacaacag ctgcccttga aggtggtgtt gaagcgctag caacagcatc
     1261 aggtatgact gcagtgactt atacgatttt ggcgattgcc catgctggtg accatgtagt
     1321 ggctgcttcg actatttacg gtggaacctt caatcttttg aaagaacccc ttcctcgtta
     1381 tggtatcaca acaacctttt tcgatattga taatttggag gaagtagaag cagctatcaa
     1441 agacaatacc aagcttgtct tgattgaaac cttgggtaac cccttgatta atattccaga
     1501 cctggaaaaa ctggcagaga ttgctcataa acatcaaatc ccacttgtgt cagacaatac
     1561 ttttgcaaca ccttatttga ttaacgtctt ctctcatggc gttgacattg ccattcactc
     1621 tgtgactaag tttatcggtg ggcatggtac aactattgga ggaataattg tcgatagtgg
     1681 tcgttttgac tggacggctt cagggaaatt ccctcaattt gttgacgagg gtccaagctg
     1741 ccacaatttg agctatactc gtgatgtggg tgcagcagcc tttattatag ctgttcgagt
     1801 tcaattgctt cgtgatacag gtgcagcctt gtcaccattc aatgctttcc tcttgctaca
     1861 aagacttgaa acctcttcac ttcgtgtgga acgccatgta caaaatgctg agacaattgt
     1921 tgattttctt gtcaaccatc ctaaggtaga aaaggtaaat tatccaaaac ttgcagatag
     1981 tccttatcat gccttggctg agaaatactt gccaaaaggt gtcggttcaa tctttacctt
     2041 ccacgtcaaa ggtggcgagg aagaagcacg caaggtcatt gataatttag aaatcttttc
     2101 tgaccttgca aacgcggcag atgctaaatc gcttgttgtc catccagcaa caaccactca
     2161 cggtcaattg tcagaaaaag acctagaagc agcaggtgtc acaccaaact aaattcgttt
     2221 gtcaatcggt cttgaaaatg tagaagattt gattgaagac ttgcgcttgg ccttggaaaa
     2281 aatttaaagt aaaagaagat aaacagtggg cttcgactca ctgtttttga ttttccctca
     2341 ggcatgatat aatggttaca gaagtctaga aagaggaacg atatgaacga aatcaaatgt
     2401 cccaactgtg gggaagtctt tacagtaaat gagagtcagt atgccgaact cttgtcccaa
     2461 gtgagaacgg cagagtttga taaggaacta cacgatagga tgaagcagga actggccttg
     2521 gctgagcaaa aggccatgaa tgagcaacag actaaactgg ctcagaagga tcaagaaatt
     2581 gcgcaattac agagtcagat ccaaaacttt gatacagaaa aagaattggc caagaaagag
     2641 gttgaacaga caagccatga ggctctcttg gctaaggaca aggaagtaca gctcttagaa
     2701 aatcagttgg ctaccttgcg tttggagcat gaaaatcaac tacaaaagac cctttctgac
     2761 ctagaaaaag aacgggatca ggttaaaaac caactacttt tgcaggaaaa ggaaaatgaa
     2821 ttatctttgg cttctgttaa gcaaaactac gaagcccagc tcaaggcagc tagtgaacaa
     2881 gtcgagtttt ataagaattt taaggctcaa caatctacaa aagcgattgg ggaaagccta
     2941 gaacagtatg cagagagtga gtttaacaag gttcgtagtt tcgcctttcc aaatgcttac
     3001 tttgagaagg ataacaaggt ctcttcgcgt gggtctaaag gggactttat cttccgtgag
     3061 tgtgatgaaa atggagttga aatcatttct atcatgtttg agatgaaaaa cgaagcggac
     3121 ggaacagaga agaagcacaa gaatgcagat ttttacaagg aattggacaa ggaccgtcgg
     3181 gagaagaact gtgagtatgc cgttttggtg accatgcttg aggctgataa tgactacttt
     3241 aacacaggga ttgttgacgt cagtcacgag tatgaaaaaa tgtatgttgt tcgtcctcaa
     3301 ttctttatcc aattgattgg tctcttacgt aatgcggcgc taaattccct aaaatacaag
     3361 caggagttgg ccttggttcg cgagcaaaat attgacatta cgcattttga ggaagatttg
     3421 gatgccttta agctagcttt tgctaagaac tataattcag cttcgactaa ctttggaaaa
     3481 gctattgatg aaatcgacaa ggccatcaaa cgcatggaag aggttaagaa attcctgacc
     3541 acatctgaaa accaactccg tttagctaac aacaaattgg aagatgtctc tgttaaaaaa
     3601 ttgacccgga aaaatccaac aatgaaagcg aagttcgaag cactgaaggg ggagtagaaa
     3661 gcaaaaatga acggtattat taacttaaaa aaggaagcag gaatgacctc gcatgatgcg
     3721 gtttttaaac tgcgtaagat tttgggaacc aagaaaattg gtcatggtgg aaccttggat
     3781 ccggatgtgg tgggtgtttt gccgattgcg gttggcaagg cgacacgcat ggtcgagttt
     3841 atgcaggacg agggtaagat ctatgagggg gaaatcactc tgggctattc cacgaagact
     3901 gaggatgcta gtggggaagt ggtcgcagaa acccctgttt tgtctctctt ggatgaaaag
     3961 cttgttgatg aagcgattgc tagcttgact gggcctatta ctcagattcc ccctatgtat
     4021 tcggcagtta aggttaatgg tcgcaagctc tatgagtatg cgcgtgctgg tcaggaagtg
     4081 gagcgtccag aacgtcaggt gaccatttat caatttgagc gaacaagtcc gatttcttat
     4141 gatggccaac ttgcccgatt cacttttcgt gtaaaatgca gtaaagggac gtacatccgt
     4201 actttgtcag ttgatttggg tgaaaagctt ggttatgcgg ctcatatgtc ccatttgact
     4261 cgtactagtg ctgctggctt acaattagaa gacgctcttg ccttggagga aattgctgaa
     4321 aaagtagagg ctgggcaatt agattttctc catcctttag agattgggac aggtgacctt
     4381 gtcaaagttt tcctaagtcc agaagaggct acagaagttc gctttggtcg ttttattgag
     4441 ctagaccaaa cggacaaaga actggctgcc tttgaagatg ataaattgtt agccattcta
     4501 gaaaaacggg gcaatctcta taagccaagg aaggttttta gctagatcgt ttaggaataa
     4561 aaatcgggtg atagataaca attgcttgat aaaaccccat actaatagta gaatggtttt
     4621 gggaattata atattccaat tgttgcgagt tgtaggtact caaataatct atatagaaat
     4681 ttagaggtgt gaaatgaagc aatttaaaat tctttcagat aaatatttag agtccattac
     4741 aggttctgat gggaacttag gcccaggatt tggtgtgata attccatgat gcgaaatgag
     4801 tttcgagaaa gggtggagca acttcttcaa caaaaagaaa taaatgaaaa tagtgagttg
     4861 agtcacctgt ttcgtcttgc tatacaaaat ttagacagaa atgaaaaata ccaatcggtc
     4921 atggccaatt tgagtcaagg gttgtcactt tacctcatga cgcatcatta ccaggcacct
     4981 aagtctgtca ttgattttgg tttatggatt gccaaagctc ctagccagga aagagggaga
     5041 ctggctttct tgcagatgct tgctcaaacc ctacagggct ttcgttaaaa ctgtcgaaca
     5101 tcagagggaa gactctggtg ttttttgctg atgagactat aaattttgta aaaaatgtga
     5161 tagaatagac gacggataaa ataacggagg atagcatgca aaatagacca atcattattg
     5221 gagtgacagg tggttctggt ggtggtaaaa ccagtgtttc aagagccatt ttatcgcatt
     5281 ttcctgatga aaagatttcc atgattgagc atgattcata ctacaaggat cagtctcatt
     5341 tgacctttga agagcgtgtc aaaaccaact atgaccatcc ttttgccttt gatacagact
     5401 tgatgatcga gcagattaag gaattgttgg cagggcgtcc ggtggacatc ccgacctacg
     5461 actatacaga gcatacacgg agtagcaaga cctatcgtca ggaacctcaa gatgtcttta
     5521 tcgttgaggg tattttggtc ttggaggaca agcgtctgcg cgatttgatg gatatcaaga
     5581 tttttgtgga tacggatgac gatgtgcgca ttattcgtcg tatcaagcgt gatatggagg
     5641 agcgtggccg tagccttgat agcgttatta accagtactt aggtgtggtc aaaccaatgt
     5701 accaccagtt tatcgagtca actaagcgtt atgctgatat cgtcattcct gaaggggtta
     5761 gcaataccgt ggctatcgac ctgttgacga ccaagattgc aaagattttg gaagaagctc
     5821 gaaacagcaa ataatcacat gtggaggcct tgcctccttt ttctattttt ccctcataat
     5881 ggtacataaa tggagatttt taggcatatt tttggtataa taatacccat gaaagagcaa
     5941 gtaaatgaat agtaggtgga gatggaaaag tatttatcgg taacaacttt gaccaagtat
     6001 ctgaaaatga aattcgataa agacccatac ttggaacggg tctatttaac tggtcaagtt
     6061 tccaactttc gtaaacgacc tactcaccaa tatttctccc taaaggatga ccatgcagtt
     6121 attcaagcga ccatctggtc tgggatttat cagaaattag ggtttgacct ggaagaagga
     6181 atgaagatca atgtgattgg gcgtgtacag gtctatgaac caagtggtag ctactccatc
     6241 atcattgaaa aagctgagcc tgatggggtt ggggcgcttg cgattcagtt tgaacaactt
     6301 aagaaaaaat tgacagaaga aggcctgttt caagaacgct tcaagcaagc tctgccccaa
     6361 ttttctaaga gaattggtgt agtaaccagc cgtagtggag ccgttattcg agatattatc
     6421 acgaccgtca gcaggcgatt cccaggtgtt gacatccttc tttatccgac caaggttcaa
     6481 ggtgaagggg ctgcggagga aattgctaga aatattgcgc gtgctaatca acgggacgat
     6541 ttggatttgc tcattattgg tcgtggaggt ggttctattg aggatctctg ggcctttaac
     6601 gaagaaattg tggtacgagc tatttttgaa tctcgtttgc cagttatctc tagtgtgggg
     6661 catgagacgg atgtgacctt ggcagatttt gtggcagatc gacgcgctgc aacgccaaca
     6721 gcggcggctg aactggcaac acctgtgacc aagttggatg tattagctca tttgcaaaat
     6781 caggaaaaac ggatggtaac ggcagtccga aatgttctat ctaagaaaca agaggctttg
     6841 aaaaaatgca gtcagtctgt tatctttaga caacctgagc gcttgtatga cggttatttg
     6901 caacgcttgg accaactgca actgcgtttg aaacaaagtt tgcgaactcg gatttctgat
     6961 aacaaacaat tagttcaagc aagaactcat caattagtac aattatcacc tgttaccaaa
     7021 atccaacgct atcaagaccg tttaggacag ttggacaagc tcttaggtag ccaaatggcg
     7081 ttagtttatg acgccaaggt tgctgaggcc aagcgacttt cggaagcttt gctcatgttg
     7141 gatactagcc gaatcgtggc gcgtggttat gctattgtca aaaaagaaga gtccgttgta
     7201 gattcggttg agagtttgaa gaaaaaagac caagtaacgc ttttgatgcg agatggtcaa
     7261 gtagaattag aggttaaaga tgtcaaaaca aaagaaattt gaggaaaatc tagcagaact
     7321 ggaaaccatt gtccaaagtt tggaaaatgg tgaaattgct ctggaagatg cgattactgc
     7381 ctttcaaaag ggcatggtct tgtcaaaaga gctccaagct acgctggaca aggctgaaaa
     7441 gaccttggtc aaggtcatgc aagaagacgg aacagaaagt gattttgaat gaaaaagcaa
     7501 gaaaaattag ctcttgtcga gtcggctttg gaagattttt atggagacca gcagtttgcc
     7561 tctagtttac gggagtctgt tctctattct attcatgctg gtggcaagcg tattcggcct
     7621 tttctcttgt tagaagttct ggaagccttg caggttacca tcaaacctgc tcacgcgcag
     7681 gtagctactg ccttagagat gattcataca gggagcttga ttcacgatga ccttcctgct
     7741 atggatgatg acgattatcg aagagggcgg ttaaccaatc acaagaaatt cggtgaagct
     7801 atggccattt tggctggaga tgccttattc ttagactcat atgccttgat tgcgcaggca
     7861 gatttgccaa gtcagattaa ggtggacttg attgccaact tatcccttgc ttcaggtagt
     7921 ctgggtatgg tagcagggca agttttggat atggagggcg aacaccagca cttgtctttg
     7981 gaagaacttc agactattca tgccaataag actgggaagt tactagccta tcccttccaa
     8041 gcggcagcta ttatagctga attgtcacct gaaatgcagg tgaagctgaa aactgtgggt
     8101 gaattgattg gacttgcttt tcaagtcaga gatgatgtac tggatgtgac agctagtttt
     8161 gaggaaatcg gcaagacacc tcaaaaggat ctgcaggcag aaaaatcaac ctatcctgcc
     8221 ctgttgggct tggaagagtc cattgccttt tgtaaccaaa ccctggatca agctaatgaa
     8281 aaattagaag aaattgccca gcagcttccc tttgaaacag aatcgattgt aagtgtagta
     8341 gaaagtttga gaatcaatgg ctaaggaaag agtggatgta ctagcttata aacaggggtt
     8401 gtttgaaacg agagagcagg ccaagcgagg tgtgatggct ggcctagtcg tagcagtcct
     8461 taatggagaa cggtttgaca agccaggaga gaaaattcca gatgacaccg aattaaaact
     8521 caagggggag aaactcaagt atgtcagccg tggtggtttg aaactggaaa aggccttgca
     8581 ggtctttgat ttgtcggtgg atggcgcgac tacgattgat atcggggcct ctactggagg
     8641 ttttaccgat gtcatgctac agaatagtgc caagttggtc tttgcagtcg atgttggtac
     8701 caatcagttg gcttggaaat tacgccaaga cccacgagtt gtcagcatgg agcagttcaa
     8761 tttccgctat gctgaaaaga ctgatttcga gcaggagccg agctttgcca gtattgatgt
     8821 gagtttcatt tcccttagtc tgattttgcc agccttgcac cgtgtcttgg ctgatcaagg
     8881 tcaggtggta gcacttgtca aacctcagtt tgaggcagga cgtgagcaga ttgggaaaaa
     8941 tggaattatt cgagatgcta aggttcatca gaatgtcctt gaatctgtaa cagctatggc
     9001 agtagaggta ggtttttcag tccttggctt ggacttttct cccatccaag gtggacatgg
     9061 aaatattgaa tttttagcgt atttgaaaaa agaaaagtca gcaagcaatc agattcttgc
     9121 tgagattaaa gaagcagtag agagggcgca tagtcaattt aaaaatgaat aaaaaagaga
     9181 gacttgaaaa aattagaaga ttggtgacag attatcaaat cggcacgcaa gaagaaattg
     9241 tagaacattt gaaagaagca ggtatcactg ccactcaggc gacggtatcc cgagatatca
     9301 aagagttagg tattgtcaaa attcctttga gagacaacac ctatgtctac gaattgccaa
     9361 aatcaatcgt aaaaagtctg caactggctg aagacaatat cgaatcggct gaattgatgg
     9421 ataagatgat caatctccaa gttattccag gaaatacggc ttttgtaaaa gctcagttaa
     9481 tcgagacttt tgcagacaag atttttagct gtttgactga tgatagctcg attttagtca
     9541 ttgccagaag tggaagtcta gcagaggaaa tctttgaaca agtaaaaaat tggtaggtct
     9601 atatgttact tgaaatttcg ataaaaaact ttgccattat tgaggctatt tccctcaatt
     9661 ttgaaaaggg gatgactgtc ctgactggtg aaacgggtgc agggaagtcg attatcattg
     9721 atgccatgaa tatgatgttg ggagctcgtg cgacgacaga tgttattcgt catggtgcac
     9781 caaaggcaga gattgagggg cttttctcag ttgagaatag tcgtctttta caggaaattt
     9841 ttgatgagca aggtttagaa ttaggtgatg aaattatcat ccgtcgggaa atcttgcaaa
     9901 atggtcgtag tatcagccgt gtaaatggtc agatggttaa tctgtctgtt ttgcgagcta
     9961 ttgggcaaca tcttgtagat attcatggtc agcatgacca tgaggagtta atgcgtcccc
    10021 aactgcatat ccagatgttg gatgaatttg gtgatgccgc tttttgggac ttgaaagaaa
    10081 cctatcaaac gagttttgat gcctatcgga aaatgcgcaa gcaggttctg gaagtcaaga
    10141 aaaaccaaca ggaacacaag gcacgtatcg aaatgttgga atttcaaatg gcagagattg
    10201 aggcagcaaa cttgcaggct ggagaagact tggctctcaa tcaagagcga gataaactcc
    10261 tcaaccacaa aaatattgcg gatacactga ccaatgccta cagtatgttg gacaatgaag
    10321 atttttcaag tctggccaat gttcgttcag ctatgaatga catggaaagt gtcgaagagt
    10381 atgatcctga ataccgtgaa atttcaagct atctgtctga gacctactat gttttagaag
    10441 acattagcaa acgtttggaa gctatcattg aggaccttga ttttgatggc aatcgcctga
    10501 tgcaggttga gaatcgtttg gacctccttc atactattac tcgtaagtat ggtgggactg
    10561 ttgatgatgt tttgctttat tttgccaaga ttacggaaga atacaatctc ttgacaggca
    10621 ataatctttc gtctgaggac atggaagcag agcttaagaa gttggaagtc aatcttgtca
    10681 atttggcagg tcaacttgct tctgctcgtc ataatttggc tcagcaactc gaagctgaga
    10741 ttaaacaaga actgcaagat ctttatatgg aaaaagccca gtttcaggtt cgttttagta
    10801 agggaaaatt cagtcgtgag ggaaataaaa tggttgagtt ttatatttca accaaccctg
    10861 gagaagactt taaacccttg gttaaggttg cttctggagg ggaattatct cgtctcatgt
    10921 tagccattaa gtctgccttt tcacgtaaag aaggcaagac tagcattgtc tttgatgagg
    10981 tggatacggg agtttcaggt cgtgttgctc aagctattgc tcagaaaatt cataaaattg
    11041 gtcagcatgg tcaggttttg gctatctccc atttgccaca agtaattgcg attgcagatt
    11101 atcaattctt tattgagaag attagtaatg accattcaac ggtttcgact gttcgtctct
    11161 tgacggtcga agagcgagtg gaggaagttg ccaagatgtt ggcaggtgat gatgtgacag
    11221 aagcagccct gacgcaagca agagaattgt tgagaaacag ggagaaataa gatgacagat
    11281 tattatgtaa ttggagatgt tcacggaaaa gctgggatgc tggaagacct tctcaaaacc
    11341 tgggatggtc agccccagtt gctctttcta ggtgatttga ttgacagagg tgaggatagt
    11401 caccgtgttc ttgaaatggt caaggacttg gtggacaatc aaggggctat ctgtttgtcc
    11461 ggaaaccacg agtatatgtt tttgacttgg ctagatgacc cagaagaaag ttatgaccat
    11521 tatagtcgca atggtggaga tacaaccatt aactctatcc taggtcgtcc cttggatgca
    11581 ccagttgatg gagtagaaga tgccaagcgg gttgccgctg aagcggcaga cttggtcgaa
    11641 ttcattcgtc aaatgccatt tgtagtagag acagacaagt atatctttgt tcacgcaggt
    11701 attgatttga ccttggacga ctggcatgaa acaacagatt ataaaaaagt atggcttaga
    11761 aaaccattcc acggagctga aaatcatact ggaaaaacca ttgtcttcgg tcatacaccg
    11821 gtctatggtt tgttacagca agaacgcggt acatctgagc tttggactac agaagatggc
    11881 aagattggaa tggatggagg agctgtttat ggtggtgtcc ttcacgggat tgtctttaca
    11941 gaccaaggaa tgacagaaca ccactttatc gaaaatgacg gctttgtcgc tgaagattag
    12001 tactcctagc agggtatggt cttgtcaaaa tatcaaaaac aatttataat aaatagatac
    12061 cctgaaagga agagaatcat gaacttagaa gaattgaaaa aacgacagga gaagatccgt
    12121 aacttctcta ttatcgccca tattgaccac ggaaaatcaa ctctagcaga ccgcattttg
    12181 gaaaaaacag agacggtttc aagtcgtgaa atgcaggccc agcttttgga tagcatggag
    12241 ctagagcggg aacgtggaat tactattaag ttgaatgcca tcgagttgaa ttacactgca
    12301 aaagatgggg aaacttatat tttccacttg attgacacac cagggcacgt tgactttacc
    12361 tatgaagttt cacgttcgct agctgcctgt gagggtgcta ttttggtggt cgatgcggct
    12421 caaggaattg aggctcaaac tcttgccaac gtttatctgg ctttggataa tgatttggaa
    12481 atcatgccta tcattaataa aattgacctg ccggctgcag atccggagcg cgtgcgtaca
    12541 gagattgaag atgtgattgg tttggatgcc agtgaagcag ttttggcttc tgccaaggct
    12601 ggtattggga ttgaagaaat cctcgagcaa attgtagaaa aagtaccagc accaacgggt
    12661 gatgtgacgg cgccacttaa ggccttgatt ttcgactctg tttacgatgc ttaccgtggg
    12721 gttatcctcc aagtgcgtgt catggacgga gtggtcaaac ctggtgataa gattcagctc
    12781 atgagcaata gtaagacctt tgatgtggcc gaagtcggta tttttacacc aaaagcggtt
    12841 ggtcgtgatt tccttgcgac tggtgatgtt ggttacattg cggcgtctat caagacggtt
    12901 caggatactc gtgtgggtga taccgttacc ttggcaacca atcctgcggc agaaccatta
    12961 catggttata agcagatgaa tcctatggtc tttgcgggtc tctacccaat cgaatcaaac
    13021 aagtacaatg acctgcgtga agcgcttgaa aaattgcaac tgaatgatgc tagtcttcag
    13081 tttgaaccag aaacatctca ggcacttgga tttggtttcc gttgtggatt tcttggactt
    13141 ctccatatgg atgttatcca ggaacgttta gagcgtgagt tcaatattga cctcatcatg
    13201 acagctccgt ctgttattta caaagttaat ttgaccgacg gtgagtctat ggatgtgtct
    13261 aacccatctg agtttccaga cccaactaag attgcgacca ttgaagagcc ttatgtcaag
    13321 gcgcaaatca tggtaccaca ggagttcgtc ggagcagtaa tggagctagc tcagcgtaag
    13381 cgtggggact ttgtgactat ggactatatt gatgataacc gtgtcaatgt tatctatcaa
    13441 attcctcttg ctgaaattgt ctttgacttc tttgataaac ttaagtcttc gacacgtggt
    13501 tatgcaagct ttgactacga attgtcagaa tatcgcccat ctaagctggt gaaaatggat
    13561 attcttctca atggagataa ggtggatgcc ctcagcttta tcgttcacaa ggactttgcc
    13621 tacgaacgtg ggaaactcat cgttgataaa ctcaagaaaa tcatccctcg ccaacaattt
    13681 gaagttccaa tccaagcggc tattggacac aagattgtcg ctcgtactga tatcaaggcc
    13741 cttcgtaaga acgtacttgc taaatgttat ggtggtgacg tttctcgtaa gcgtaaactc
    13801 cttgaaaaac aaaaagctgg taagaaacgc atgaaatcca tcggatcagt tgaagttccg
    13861 caagaagcct tcctcagcgt cttgagtatg gatgaagaat aagttatcag aaatgccttt
    13921 ccttgttcac aggaaattta tataagaatc aatagtaaag caggcttaga tggtctgctt
    13981 ttttagtttt tatactcttc gaaaatctct tcaaaccacg tcagcgtcgc cttaccgtat
    14041 gtatggttac tgacttcgtc agtttcatct acaacctcaa aaccatgttt tgagctgact
    14101 tcgtcagttt catctacaac ctcaaaactg tgttttgaac aacctgcggc tagcttccta
    14161 gtttgctctt tgattttcat tgagtattag aacagaaaaa tgcttggagt atttgtttgt
    14221 gtgtttattt ttatataaca aactataaac aaaacaaaaa tataaaaaaa gagacaaaaa
    14281 agaatagaaa gtaattaaca acgatttcat atagtgttat acttatagca taaagttaat
    14341 ggaaagaagg gaaaacctta tggtattaga ttatttcttt gacaaaaacc ttgtgttttg
    14401 cttagaagcg gataatcaag aataactttt tgatcaggta gcaagcttgt tggaagaacg
    14461 agaaattgtc actccaactt atcgtgaagc cttgatcacg cgtgaaaagt catttccaac
    14521 tggtttatat atggaatttc taggaaagga cttgcaaatg taatgattcc tcacacagat
    14581 attgttcata atctagctga gaaagtggtg gttgttcgat tagagaaacc ggtgactttc
    14641 cataatatga ttgcgccagg taaggaagta gaagtatcct tgctcttctt tatcatcaat
    14701 aattcaagtt caagtcaaac gaacattctt gctcagttaa tggacttctt cacaggaaat
    14761 ggtcatcttg aagacctatc aaaaatttct gaaccagaag ccctttatgc ttacattgct
    14821 gaagcaaccg cttaatcttg tctattaaaa aataaaatcg gaggaaatcc aaatgattaa
    14881 aattcttgct gcctgcggtg caggtgttaa ctcaagtcac caaattaaaa gtgctctaga
    14941 agaagaactt tcaaaccgtg gttacgatgt tcactgtgat gcagttatgg tgaaagatgt
    15001 aaacgaagac cttatgaaag gttacgatat ctttacacca atcgctgcaa cagaccttgg
    15061 ttttgaacca ggtatcccag ttatcgaagc tgggccaatc ttattccgta tcccagcaat
    15121 gagcgttcca gtatttgaca atattagact tcctgcgaaa caaaacatgg tatagtagtt
    15181 ctatgaatga tgaagcaagt aaacaactaa ctgatacacg atttaaatgt cttgttggtg
    15241 ttcagcgcac gacttttgaa gagatgttag ctgtgttaaa aacagcttat caacttaaac
    15301 acgcaaaagg tggacgaaaa cctaaattaa gcctagaaga ccttcttatg gccactcttc
    15361 actatgtgcg agaataccgc acttatgaag aaattgcggc tgattttggt atccacgaaa
    15421 gcaacttact ccgtcggagc caatgggttg aagtaactct tgttcaaagg tggtgttacg
    15481 atttcaagaa ctcctctcag ttctgaagac acggtaatga ttgatgcgac ggaagtaaaa
    15541 atcaatcgac ctaaaaaaag aattagcgaa ttattctggt aaaaagaaat ttcacgctat
    15601 gaaggctcaa gcgattgtca caagtcaagg gagaattgtt tctttggata tcactgtgaa
    15661 ctattgtcat gatatgaagt tgttcaaaat gagtcgcaga aatatcggac aagctggtaa
    15721 aatcttggct gacagtggtt atcaaggacc catgaagata tatcctcaag cacaaactcc
    15781 acgtaaatcc agcaaactca agccgctaac agttgaagat aaagcctata accatgcgct
    15841 atctaaggag agaagcaagg ttgagaacat ctttgccaaa gtaaaaacgt ttaaaatgtt
    15901 ttcaacaacc tatcgaaatc atcgtaaacg cttcggatta cgaatgaatt tgattgctgg
    15961 tattatcaat catgaactag gattctagtt tttcaggaag tctaataaag aaactagcaa
    16021 ggtcaggcct tactttattg gtggacatcc aggatttaat tgtcctctac ttgatgatga
    16081 agtctatgaa gattattatc tagagtttga gaaagaagag atttgctctg ttccacgtcc
    16141 tttcccagaa acaggtatgt tggatttcca agatagaagt ccatggctag agggacaaaa
    16201 agaaatagat cttagttatg atcttttcag cacagatgca gtgactttgg atgaattgca
    16261 atctcgaaca attgcccttc gttctcttaa acatgataag ggattgaaag tgcattttgc
    16321 agaatttcca aacctcatca tctggtcaac tttgaacaag ggacctttca ttacctttga
    16381 accatggtct ggcttgtcaa catcccttga agaaggagat catttagaag ataagaagaa
    16441 tgtttgtctc ttagaagcca atcaggttga agaattaggg tttgagatag aagttttata
    16501 aaaaagtaaa aaataaataa caaaaacaaa caaaagctgt tgacttcttc aaacaatagt
    16561 agtatattag gtttgtgagg actaattgac tgttttttaa agacagtcaa tacactgctg
    16621 gaatccagca tagaaaaaat aaaaaggagt atacaatatg tctattgtta tcggtgcaga
    16681 tgctgcaggt ttgagattga aagaagttgt gaaagacttc ttagaaaaag aaaacttcca
    16741 ccttgtggat gttacagctg aaggtcaaga ctttgttgat gtgactctcg ctgttgctgc
    16801 agaagtaaac aaagaagaac aaaaccttgg tatcgtgatt gatgcttatg gagctggtcc
    16861 atttatagtt gcaactaaga tcaaaggaat ggttgctgca gaagtatctg acgaacgttc
    16921 agcttatatg actcgtggcc acaacaactc acgtatgatc actatgggag cacaacttgt
    16981 tggtgatgaa ttggctaaaa atatcgctaa aggatttgtt aatggtaaat acgacggtgg
    17041 tcgtcaccaa atccgggttg acatgttgaa caaaatgggc taattagatt atagtaagaa
    17101 aggtaagtta aaaatgagaa ttgcaattgg atgtgaccac atcgtaactg atgaaaaaat
    17161 ggcggtttca gaatttttga aatcaaaagg atatgaagtc attgactttg gtacctatga
    17221 ccatacacgg actcactacc caatctttgg taaaaaagta ggggaagctg taactagcgg
    17281 tcaagctgat cttggagtat gtatctgtgg tactggtgtt ggtatcaaca acgctgtaaa
    17341 taaagttcca ggtgttcgtt ctgccttggt tcgtgatatg acaacagccc tttatgctaa
    17401 agaacaattg aacgctaacg ttattggttt tggtggtaaa attactggtg aattgcttat
    17461 gtgtgatatc atcgaagctt tcatccatgc tgaatacaaa ccaactgaag aaaacaaaaa
    17521 attgattgcg aaaattgaac atgttgaaag tcacaatgct caacaaacag acgcaaactt
    17581 ctttacagaa ttccttgaga aatgggatcg tggagaatac cacgactaag aggtgaccta
    17641 tgattttaac agtcacaatg aacccatcca tcgatatttc ctatcccttg gatgagttga
    17701 agattgatac tgtcaatcgt gtggtggatg taaccaaaac ggctggtggt aagggactca
    17761 atgttacccg agtactttca gaatttggcg attctgttct tgctactggt ttagtgggtg
    17821 gcaaacttgg tgagtttttg gttgaacata tcgataatca agtaaagaaa gatttcttct
    17881 caattaaggg agaaactcgt aactgtatcg ctattctcca cggagacaac caaacagaag
    17941 ttcttgaaaa aggtcctgaa gtattggaac aggaaggtca agagtttttg gaacatttca
    18001 aaaaactctt ggagtcagtt gaagtagtag ccatctcagg tagtctgcca gctggccttc
    18061 cagttgatta ctatgcgagc ttggtagaac ttgctaatca agctggcaag catgtagtct
    18121 tggactgctc aggtgcagca cttcaggctg ttcttgaatc accccataaa ccaacagtca
    18181 tcaaaccaaa taatgaagaa ttgtctcagc ttcttggaag agaagtttct gaggatttgg
    18241 atgaattaaa agaagtactt caagaacctt tgtttgcagg gattgaatgg attatcgttt
    18301 cacttggtgc caacggtact tttgccaaac atggtgacac tttctacaag gtagatattc
    18361 ctagaattca ggtggtaaat cctgttggat ctggagactc tactgtggca ggaatttctt
    18421 caggacttct tcacaaagaa tcggatgcag aattactcat caaggcaaat gtccttggta
    18481 tgctcaatgc tcaagaaaaa atgactggtc atgtcaacat ggccaactat caagctctat
    18541 atgatcaatt aatagtaaaa gaggtataaa atggctttaa cagaacaaaa acgtgtacgc
    18601 ttagaaaaac tttctgatga aaatggtatc atctcagctc ttgcatttga ccaacgtggt
    18661 gctttgaaac gcctcatggt taaacaccaa acagaagaac caactgtggc ccaaatggaa
    18721 gaacttaaag tcttggtagc agatgaattg actaaatatg cttcatctat gcttcttgac
    18781 cctgagtatg gacttccagc aactaaagct cttgatgaaa aagctggtct tctccttgct
    18841 tatgaaaaaa caggttatga cacaacaagc acaaaacgct tgccagactg cttggatgtt
    18901 tggtctgcaa aacgtattaa agaagaaggt gcagatgcag ttaaattctt gctttactat
    18961 gatgtagata gctcagacga actcaatcaa gaaaaacaag cctacatcga acgcatcggt
    19021 tctgagtgtg tggctgaaga tatcccattc ttccttgaaa tccttgctta cgatgaaaaa
    19081 attgcggatg caggttctgt agaatacgct aaagtaaaac cacacaaagt tatcggcgct
    19141 atgaaagtct tttcagaccc acgctttaac attgatgttt tgaaagttga agttcctgtt
    19201 aacattaaat atgttgaagg cttcgctgaa ggtgaagtag tttatacacg tgaagaagca
    19261 gcagccttct tcaaagcgca agatgaagca acgaacttgc catacatcta cttgagtgct
    19321 ggtgtatcag ctaaactctt ccaagatact cttgtatttg ctcatgaatc aggtgcgaac
    19381 tttaacggag ttctttgtgg ccgtgctaca tgggcaggat cagttgaagc ttacatcaaa
    19441 gatggtgaag cagcagctcg cgaatggctt cgcacaactg gatttgaaaa cattgacgaa
    19501 ctcaacaaag ttcttcaaag aacagcaact tcatggaaag aacgcgtgta agaaagtcct
    19561 cctagtttag gaacatgaat ctaaaaaaat ttaaaaaaag ttgtatgtaa aggcttacaa
    19621 aataacttac ttgtgctata cttaaatcac aagttaatat gaattagaaa gtaactatat
    19681 gaagtataat aaaaatagga tatagtttat tttacgagct aggaaggaaa aatacggaaa
    19741 caatattgcc agaataaact atatttagat gcacatttca ttcattgttt tataaaagga
    19801 gaagataaac ggctactaaa aagagtttta aagcgttagt tgtaggacta ggtattgttt
    19861 caatattctt atcagcctta cctatggtta gtggttctgt atttgcagat agtgccctaa
    19921 ctacagtaga taaagcaaat gatattgttt tgaatgttga tgggaataaa ttttataatg
    19981 tttcggtttc agaagatatt gtaaatgctg gtcaaatttt ggaagattat ttttatgtag
    20041 ataaatttgg aaatataaat ttaaaaggca ctcctgaaga gttagcaaaa aatattggta
    20101 tttctgtaca agaagcaagt ttgatgtatg gagctgtaaa agagttaccc aacgtttacg
    20161 aaagaggtcc tgtaggtttt cgtttcaatc ttggtcctca agtgaggggg atgggtggct
    20221 gggctgctgg agctttcgct actggatatg ctggatggca tttgaaacaa tttgcggtta
    20281 atcctgttac atctggattt gttgctgtaa taagtggtgc gattggctgg gctgtaaaaa
    20341 ctgctgtaga aaattattgg acagttgctg tagctacagt agaagtgccg tttgtgaacc
    20401 ttgtttacac catagattta ccttagaggt tatttcttta tgaatcattc ttttaaaaaa
    20461 ataactgtat tttgttttat agtttcttgt gttctttgtt tattagactt aatgaatttt
    20521 aaaaatgtag ctactttttt atttttctgt cttcctgttt ttgttttgat ttacaaaaat
    20581 aaataaaaac agagcctctg tttgatgaat tttagaacat agttaagttt taaaaaaagt
    20641 tgtatgtaaa ggtttacaaa ataacttact tgtgctatac ttaaatcaca agttaataca
    20701 aggtgagtgt tactaagtaa tattaggcat gatcacaggt gaattagaaa tcagctgatt
    20761 ttctagttca tttgtggtca ttttttgtac ttatatacct ttaagatata aaaggaggtt
    20821 gacatgtatc gaattctaaa tccaatgaat cacaatgtct cgcttgtcag aaatgataag
    20881 ggagaagagg tgattgtaat tggtaaggga attgcattcg gaaagaagaa gggggatttg
    20941 attgctgaaa atcaggttga gaaaatcttt cggatgaaga ccgaagagtc cagagaaaac
    21001 tttatggctc ttctcaaaga tgttccgctt gattttatca cagtgaccta tgaaatcatt
    21061 gataagctat caaagaaata tcattatccg attcaagagt atctctatgt aaccttgaca
    21121 gatcatattt actgttctta tcaagctcta actcaaggaa ggtacaagga tagtaatctg
    21181 ccagatattt ccgctaagta tcctgtcgct tttcaaatcg caaatgaagc ttttgaaatt
    21241 taccgtcaga agctagcaga tcattttcct gaggacgaaa ttattcggat tgcttatcat
    21301 ttcattaatg ctgaaggtga aaatgaagtg gaacttgtgg agtcgattga taagaggaaa
    21361 gaaattctca ggaatgttga agaagtttta acggactatg caattcaacg aactaaaaag
    21421 aataaccatt tctatgatcg ctttatgatc catttgaatt atttcttgga ttatttagac
    21481 agatctagag atgataacca atcacttctg gatatggaag atcatattaa acaatcctat
    21541 ccaaaagcct tcgagattgg ttccaagatc tatgatgtga ttacgcaaca tacgggtctt
    21601 gatttgtata aaagtgaacg agtttatcta gttctacata tccaacgttt attgtcataa
    21661 aaatttattt aaaactatat aaggagaatt ctatcatgaa tagagaagaa gtaacattgt
    21721 taggttttga aatcgtagcc tatgctggcg atgctcgttc aaaactattg gaagccttga
    21781 aggctgctga agctggtgat tttgaaaaag cggacgctct ggtagaggaa gctggtagct
    21841 gtattgcaga ggctcaccac gcgcaaacaa gtctattgac taaggaagct tcaggtgagg
    21901 acttggctta tagtgtaacc atgatgcatg gccaagacca cttaatgaca actatcttgt
    21961 taaaagattt gatgcatcat ttaattgaac tctacaagag aggagttcaa taatgaataa
    22021 actaattgca tttatcgaga aaggaaagcc tttctttgaa aaactatctc gtaatatcta
    22081 tcttcgtgct attcgtgatg gtttcattgc aggtatgcct gttattctct tctcaagtat
    22141 ctttatcttg attgcctttg taccaaactc atggggcttt aaatggtctg atgaagttgt
    22201 agcctttctg atgaaacctt atagctattc tatgggtatt ctggctctct tggtagctgg
    22261 tacaacagct aagtcattga ctgactcagt aaaccggagc atggaaaaaa ccaatcaaat
    22321 caactatatg tcaacattgt tggcagcaat tgttggtttg ttgatgttgg cagctgatcc
    22381 tatcgaaagt ggtctagcta ctggattctt ggggacaaaa ggtttgcttt cagccttcct
    22441 tgctgccttt gttactgtag ccatctataa ggtttgtgtt aagaacaacg tcactattcg
    22501 tatgcctgac gaagttccac caaatatctc acaagtcttt aaagatgtga ttccattcac
    22561 tctatctgtt gtttctcttt atgctcttga cttattagca cgttattttg ttggttctag
    22621 tgtggcagaa tcaatcggta aattcttcgc accactcttc tcagcagcag acggatacct
    22681 tggtattacc attatctttg gtgcctttgc cttcttctgg tttgttggga ttcatggtcc
    22741 atctatcgtt gaaccagcta tcgcagctat tacctatgcc aatgccgaag ttaacttgaa
    22801 ccttctccaa caagggatgc atgcagacaa gattcttact tctggtacac aaatgtttat
    22861 cgttaccatg ggtggtacag gtgcgacatt ggtcgttcca tttatgttca tgtggttgac
    22921 aaaatcgaaa cgtaaccgtg caatcggacg tgcttcagta gttcctacct tcttcggtgt
    22981 aaatgaacca atcttgtttg gtgcacctct tgttttgaat ccaatcttct tcattccatt
    23041 tatctttgct ccaattgcaa acgtatggat tttcaaattc tttattgaaa ctcttggaat
    23101 gaactcattc actgctaatc taccatggac aactccagct ccactaggtc tagttcttgg
    23161 aactaacttc caagtgctat cattcattct tgctgccctt ctaatcgtgg ttgacgttgt
    23221 catttactat ccattcctta aggtctatga tgaacaaatt cttgaagaag aacgttcagg
    23281 taagtctaat gatgaattga aagaaaaagt tgctgcaaac ttcaacactg caaaagcgga
    23341 tgctattctt gaaaaagcgg gtgtcgatgc agcacaaaat accatcactg aagaaacaaa
    23401 tgtcctcgtt ctctgtgcag gtggaggaac aagtggtctc cttgcaaatg ctttgaataa
    23461 ggcagcagca gaatacaatg tccctgtgaa agcagcagca ggcggctatg gtgctcaccg
    23521 tgaaatgtta ccagagtttg atcttgttat ccttgcccct caagttgctt caaactttga
    23581 agatatgaaa gcagaaacag ataagctcgg tattaaacta gcgaaaacag aaggcgctca
    23641 atacatcaaa ttaactcgtg atggaaaagg tgctcttgca ttcgtacaag cgcaattcga
    23701 ttaaggctag agactctgaa atagtctccc atcgttacgg aaatcgctat ggcgaatttc
    23761 ctattattaa ttcgtcggta aaaagatatc gtttttacct cctcatgtca caattcggtg
    23821 acttggtaca agaagtgaga tggagaagga tggctcactg actcctctcc tctcactttt
    23881 actttattta aatcaagaaa taggtgaaaa aaatgacaaa aacacttcca aaagacttta
    23941 tttttggtgg cgcaacagct gcttatcaag cagaaggtgc tacacatact gatggaaaag
    24001 gaccagttgc ttgggataaa tatcttgagg ataactactg gtacactgcc gaaccagcta
    24061 gtgattttta caatcgatat ccagttgacc tcaagctagc agaagagtat ggtgtcaatg
    24121 gtattcgaat ttctattgct tggtcacgta ttttcccgac tggttacggc caagtaaatg
    24181 ctaaaggtgt tgagttttat cataatttat ttgcagagtg tcacaaacgt catgttgagc
    24241 cttttgtaac tcttcatcac tttgacacgc cagaagctct ccactcaaat ggagacttct
    24301 taaaccgtga aaatatcgaa cattttgtag actacgctgc cttctgtttt gaagaatttc
    24361 cagaagtaaa ctattggaca acctttaatg aaattggacc aatcggtgat ggtcaatatt
    24421 tggttgggaa attccctcca ggtatccagt acgaccttgc caaagtcttt caatcacacc
    24481 acaatatgat ggtgtctcat gcacgcgcgg taaaattgta caaagagaaa ggctataaag
    24541 gggaaattgg tgttgttcac gccctgccaa ctaaatatcc tctagatcct gaaaatccag
    24601 cagatgttcg tgcagctgag ttggaagata tcatccacaa taaattcatc ttagacgcaa
    24661 cttatctagg tcgctattca gctgaaacca tggaaggtgt caaccatatc ttattagtca
    24721 atggtggtag tttggatctt cgtgaagaag attttacagc attagaagct gcaaaagact
    24781 tgaatgattt cctaggaatc aactactata tgagtgactg gatggaagcc tttgatggag
    24841 aaactgaaat tatccataat ggtaaaggtg aaaaaggaag ctctaagtat caaatcaaag
    24901 gtgttggtcg tcgtgtagct cctgactatg taccacgcac ggattgggat tggattatct
    24961 accctcaagg tttgtatgac caaatcatgc gtgtgaagaa agattatcct aactacaaga
    25021 agatttacat cactgaaaat ggtctcggct ataaagatga gttcgttgat aacactgttt
    25081 acgatgatgg tcgtattgat tacgtgaagc aacacttgga ggttttatct gatgcgattg
    25141 cagatggagc taatgtaaaa ggttacttca tttggtcatt aatggatgtc ttctcatggt
    25201 caaacggtta tgagaaacgt tatggtctct tctacgtaga ttttgaaact caagaacgtt
    25261 atcctaagaa atcagctcac tggtacaaga aagtagcgga aactcagatt atagactagt
    25321 agaattagtc attagatata gaattttagt gagtcaaaaa gatgttcaaa gattttatcc
    25381 aatctattta tgaaaaaaag tttatattat aaatttcgaa aaatgctctc aaataccgtg
    25441 tttgacgagt gaagaattga aaagtcttgg aaaatggtat gtctcgactg gtaaagaatg
    25501 gatttgtcat tcagatgatg agctggaaga atttaaaaat ctatttttaa attttatcaa
    25561 tcctgaagaa tgggatacta tctcctttga ttcagatttt atgccgtttc aacaatcgta
    25621 accaatttct caaaaaagtt aaatcttata tttagtactc tgtaaaactc ttatctaatc
    25681 acgttgctta tactcaatga aaatcaaaga gcaactttaa actaggaagc gagtcgcaga
    25741 tttctcaatg catagctttg aggaattggg caaaaagtct ttgatataga aaaacgcata
    25801 gtatcaggtg tttcaacacc tgatactatg cgttttattg tgggaagatt tacttttttt
    25861 cttctgaaat tgagttgtta cccaggctct ttcagtttat taaggcttga tgactttaat
    25921 gtgtttagat agcttaaaaa ggattgaatc acttagttta gaatctgaaa caatagtatc
    25981 aagatttgat acattataaa aagtataaaa atcaaactta ttgaacttgc tatgatctgc
    26041 gagtaaatat tttttattag aattatttaa agcgatgcgt tgagcctctc cctcttcctc
    26101 gctaaaagta gctagagctc cgttttgaat accattacag ctaacgaaag ctttagaaaa
    26161 ttggagatta gagagatttt gtagggtcaa tgtaccaaca aaagcacctg taatatcgcg
    26221 ataatttcca cctattaaaa tcaaatctgt taattttcgt tcgcttaaaa tcagaaaaac
    26281 aggtagactg ttggttacga cgcggatatt gtcaataggc aactcacgcg caaaaaactc
    26341 taatgttgtt cctggtccaa tgaaaatagt ttctctttct tctactagac tgcctgcaaa
    26401 atgggctatt tcttgttttt ctgccgtttg gagggcttgt ttttcaatat ttgatcgctc
    26461 attagtcaaa agggagttgg ttcgaagttt ttcagctcca ccatgcacac gaatcagcaa
    26521 atctttatca gctaattcct gtaaatagcg ccttgcagtc atatctgaaa cggctatttc
    26581 gtccataatc tgtttaactg ttatggttcc tttactattt actatctcta aaattttggt
    26641 taatttttct tgtttgagca tattatcacc tcgtttccta ctactatctt accataaact
    26701 agctcatcat tcaaatacaa aaacaacaaa ataaaacaaa aacaaaaata tcgaggttta
    26761 ttttcaaaac tttcgatatt tttattaagt tattattttg ttgtttctag tttacttttt
    26821 gatggttaag agtggtggag aattatactc aatgaaaatc aaagagcaaa ctaggaagct
    26881 agccgcaggc tgtacttgag tacggcaagg cgaagctgac gtggtttgaa tttgattttc
    26941 gaagagtatt agtgcaaacc gtagttgtag tcatcatctt gcatggcttc aacttcgcca
    27001 agaaggtaac catttccgac ttgagagaag aagtcatggt tggaagttcc tgttgaaata
    27061 ccgttcataa cgattgggtt gacatcttca gctgaatctg ggaaaagtgg atcttgtccc
    27121 atgttcatga gagctttatt ggcattgtag cgaaggaagg ttttaacctc ttcagtccaa
    27181 ccaacaccgt cataaagact ctctgtgtag ccttcttcat tttcataaag agtatagagt
    27241 aggtcgtaca tccattcttt gagtttttct tgctcttctt caggtaattc attgaaacca
    27301 agttggaatt tgtaaccaat gtaggttccg tgaacagact cgtcacgaat aatcaattta
    27361 atgatttctg caacgttggc aagtttgttg ttaccgagat agtagagggg agtgaagaaa
    27421 ccagagtaga agaggaaggt ttcgaggaag acgctggcaa ctttcttttc aagtgggctg
    27481 ccgtttaggt agatttcgtt gacaatctca gccttctttt gtaggtaagg attggtattg
    27541 gtccattcga aaatttcttc aatctcagcc ttagtattca aggtagaaaa gattgatgag
    27601 taagatttag cgtggacaga ttccataaat tggatgttat tgaagacagc ttcctcatgt
    27661 ggtgtacgga tgtctgcgcg aagggcttga accccagttt cagattgcat agtgtcaaga
    27721 agggttaaac caccaaaaac ttttccgacc aagtctttct ctttgttaga tagctttctc
    27781 cagtcatcca agtcgtttga taagggaata cgtgtatcga gccaaaattg ctccgtcagt
    27841 ttttcccaag ttgatttgtc gatgacatct tcgatggcat tccagttaat ggctttgtag
    27901 taagtttcca tttaaaatct ctttctgtgt ttagtattgc gaactcacaa ttatttctac
    27961 tttaccataa ttctatagga gtatcgcaca aaaagtcgga agcccgactt ttaaaatgtt
    28021 acataaatta tgttatgaca tagtagattt gattttatca gtgctgctta gggaaaaata
    28081 gtgtttctat gctagaaact aaatcacaca gctttcacat tggttggcgc cgacttctcc
    28141 accgtcatct gtaaaggtac ggacgtagta gatagacttg attcccttgt taaaggcata
    28201 gttacgaagg atggacaagt cacgtgtcgt ttgtttattt tccctcttcc attcgtaaag
    28261 gccttttgga atgtcactgc gcatgaagag ggtgagtgaa agtccttgat ccacgtgttc
    28321 agtcgcagca gcgtaaacat cgatgacttt acgcatatcc atatcgtagg cagaagtgta
    28381 gtaaggaatg gtttctgtag acaagccagc agcagggtaa tagattttac caattttctt
    28441 ctcttggcgt tcttcgatac gttgcgtaat cgggtggata gaagcagaaa cgtcgttgat
    28501 atagctgata gaaccatttg gcgctacagc aaggcgattt tggtggtaaa gaccatcttc
    28561 ttgaaccttg tcgcgaagtt cagcccaatc agcaacacca gggataaaga catttttgaa
    28621 gagttcttta acacggtctg atgttggaac aaattcacca gttacatact tgtcaaagta
    28681 acttccgtta gcatagtctg atttttcaaa gttgtggaag gtaataccac gttcacgtgc
    28741 aatattgttt gactctacca aggtccagta gttcataagc ataaagtaga tgcttgtaaa
    28801 ttcaacagac tcaggtgaac catattcaat gagttgttgg gcaaggtagc tgtgcagtcc
    28861 catggcaccg agaccaaagg tgtgggcttg gctatttcca tggtcaatcg ttggtacagc
    28921 tacgatatgt gaactatctg taacgaaagt aagggcacga accatagcac ggatagaacg
    28981 accaaaatca ggtgaagtca tcatgttaac cacgttggtt gaacccaggt tacatgaaac
    29041 atctgttccc atttgaagga attcttgagc atcgttgatc aagcttggtt cttgaacttg
    29101 aagaatctca gaacacaagt tactcatgat aatctttcca tcaacaggat ttgcacggtt
    29161 agccgtatcg atgttgacta cataaggata gccagactct tgttgcaatt tagagatttc
    29221 agtttccaaa tcccgcgcct tgatttttgt cttgcgaata tttggatttg cgaccaattc
    29281 atcgtatttt tcagtaatgt cgatgtaatt gaatggcaca ccgtattctt tttctacaga
    29341 gtaagggctg aagaggtaca tttcttcatt tttacgagcc aattcgtaga atttatcagg
    29401 tactacaaca ccaagtgata gagtcttgac acgtactttt tcatcagcgt tttctttctt
    29461 agttgaaagg aaagcgatga tatctgggtg aaagacgttg aggtagacaa caccagcacc
    29521 ttgacgttgc cccaattggt tggagtaaga gaagctgtct tcaaaaagct tcataacagg
    29581 aacgacacct gaagcagctc cttcatagcc tttgataggt gcaccagctt cacgaaggtt
    29641 gctgagggta attcccacac caccaccaat acgtgaaagt tgaagagctg agttgataga
    29701 acgcccgata gagttcatat catccgtcac ttggattagg aaacaagata ccaactcccc
    29761 acgacgagca cgtccagcat tcaagaagga aggagtagca ggttggtagc gttggtggat
    29821 gatttcattg gcaatatcga ttgcaacagc ttcattccca tcagcgaaat aaagggcatt
    29881 gaagaagaca cggtcttcca tattttcaag atagtattca ccgtcattag tctttaaggc
    29941 atattgattg taaaatttat aagctgccat gaatgacttg aattggaagt tttggtcttt
    30001 gataaattga gctaattctt ccaagaactc tggacggtat ttcttgataa aggctgtttc
    30061 gatgtagttg tgttcaatga ggtaattgat tttgtctttg attgaatcaa aaaccatagt
    30121 gtttggaact acattttctt taaagaaagc atccaaggct tccttgtctt tatgaagcat
    30181 gatttgtcca ttaacaggac ggttaatttc gttattaaga cggaagtaag tcacgtcttc
    30241 aagatgtttt aatcccataa aatttccctt atctaattac aaaagaaagg cttctaagtt
    30301 agccctaaaa gcagtttctt ctggatgatg tactaagatt atgctaattg tttcagtttt
    30361 cctggttgga aacctgaaaa gacttcagtt ggtgtttgga taacaggagc tgcgctaaaa
    30421 ccgagctctt taacttgatc gacgtactca ggttgctcat caagattgat ttcacgataa
    30481 gagacattat tactgtccaa gaaacgcttg gtcattttac attggacaca attgttttta
    30541 gaataaacgg ttaccattgt gtaactcctc ttcaaaattt aatactatct tagtatatca
    30601 gaaaataaaa ttttgtcaat gatttgaact aaatatagtg gtgttctttt gttgttttta
    30661 tatataaaca caatatatag tgtttgtatt gtgaaatagc aatgattatc ctataaattg
    30721 tttttaaaga aactatatcc tgtgttttgt tatctaggat agaagatttt cagcaagtta
    30781 tctgaaagga aattgaagga aaatccagaa aatgcttgaa aaaaatccta gaagatggta
    30841 taatactaaa ttgtaagggt tatcacatat aactcaaaaa aagaaagaac aaaaggagag
    30901 tcaaactatg gcttctaaag atttccacgt agtggcagaa acaggtattc acgcacgtcc
    30961 agcaacattg ttggtacaaa ctgctagcaa atttgcttca gatatcactc ttgagtacaa
    31021 aggtaaatca gttaacctta aatcaattat gggtgttatg agtcttggtg ttggccaagg
    31081 tgctgacgta actatctcag ctgaaggtgc agatgcagat gacgctatcg ctgcaatctc
    31141 agaaacaatg gaaaaagaag gattggcata agggaaatga cagaaatgct taaaggaatc
    31201 gcagcatctg acggtgttgc agttgcaaaa gcatatctac tcgttcagcc ggatttgtca
    31261 tttgagacta ttacagtcga agatacaaac gcagaagaag ctcgccttga tgccgctcta
    31321 caggcatcac aagacgagct ttctgttatt cgcgagaaag cagtaggtac gctcggtgaa
    31381 gaagcagctc aagtttttga tgctcactta atggttcttg ctgacccaga aatgatcagc
    31441 caaatcaagg aaactatccg tgcgaagaaa gtgaatgcag aagcaggtct gaaagaagtt
    31501 acagatatgt ttatcactat ctttgaaggc atggaagaca acccatacat gcaagaacgc
    31561 gcagcggata tccgcgacgt gacaaaacgt gtattggcaa accttcttgg taaaaaattg
    31621 ccaaacccag cttctatcaa tgaagaagtg attgtgattg cgcatgactt gactccttca
    31681 gatacagctc aattggacaa aaactttgta aaagcttttg taaccaacat tggtggacgt
    31741 acaagccact cagctatcat ggcacgtaca cttgaaattg ctgctgtatt aggtacaaat
    31801 aacatcactg aaatcgttaa agacggtgac atccttgctg ttaacgggat cactggagaa
    31861 gtgattatca acccaacaga tgaacaagcg gcagaattta aagcagctgg tgaagcctat
    31921 gcgaaacaaa aagctgaatg ggcacttttg aaagatgctc aaacagtgac tgctgacggt
    31981 aaacacttcg agttggctgc taatatcggt actccaaaag acgttgaagg tgttaacaac
    32041 aacggtgcag aagctgttgg actttaccgt acagagttct tgtacatgga ttctcaagac
    32101 ttcccaactg aagatgagca gtatgaagca tacaaggctg ttcttgaagg aatgaacggt
    32161 aaacctgttg tcgttcgtac aatggatatc ggtggagata aggaacttcc ttacttcgat
    32221 atgcctcacg aaatgaaccc attccttgga ttccgtgctc ttcgtatctc tatctctgag
    32281 actggagatg ctatgttccg cacacaaatc cgtgctcttc ttcgtgcgtc tgttcacggt
    32341 caattgcgta tcatgttccc aatggttgcg ctcttgaaag aattccgtgc agcgaaagca
    32401 gtctttgatg aagaaaaagc aaaccttctt gctgaaggtg ttgcagttgc ggataacatc
    32461 caagttggta tcatgatcga gattcctgca gcggctatgc ttgcagacca atttgctaaa
    32521 gaagttgact tcttctcaat tggtacaaac gacttgatcc aatatacaat ggcagcagac
    32581 cgtatgaacg aacaagtttc atacctttac caaccataca acccatcaat cctacgcttg
    32641 attaacaatg tgatcaaagc agctcacgct gaaggtaaat gggctggtat gtgtggtgag
    32701 atggctggtg accaacaagc tgttccactt cttgtcggaa tgggcttgga tgagttctct
    32761 atgtcagcaa catctgtact tcgtacacgc agcttgatga agaaactcga cacagctaag
    32821 atggaagagt acgcaaaccg tgcccttaca gaatgctcaa caatggaaga agttcttgaa
    32881 cttcaaaaag aatacgttaa ttttgattaa tcgaaaagtc cctgcaactc agttacaggg
    32941 atttttttga tattttaaaa agaattttca agaaaatctt tcttatagaa agtccaacct
    33001 tgaaaaagta gtggtcagaa caaaaaatac ttaaatggtt cataaaattc ttgacaagtt
    33061 ggatatttag gagtaaacta ttaaccagtt aagtaataga gaggagtttc tgcaatttag
    33121 aaatgaattg caactagaaa tatcaaatag aaagagagtt tcgatgaaaa ttaataagaa
    33181 ataccttgtt ggttctgcgg cagctttgat tttaagtgtt tgttcttacg agttgggact
    33241 gtatcaagct agaacggtta aggaaaataa tcgtgtttcc tatatagatg gaaaacaagc
    33301 gacgcaaaaa acggagaatt tgactcctga tgaggttagc aagcgtgaag gaatcaatgc
    33361 tgagcaaatc gtcatcaaga taacagacca aggctatgtc acttcacatg gcgaccacta
    33421 tcattattac aatggtaagg ttccttatga cgctatcatc agtgaagaat tactcatgaa
    33481 agatccaaac tataagctaa aagatgagga tattgttaat gaggtcaagg gtggatatgt
    33541 tatcaaggta gatggaaaat actatgttta ccttaaggat gctgcccacg cggataacgt
    33601 ccgtacaaaa gaggaaatca atcgacaaaa acaagagcat agtcaacatc gtgaaggtgg
    33661 aactccaaga aacgatggtg ctgttgcctt ggcacgttcg caaggacgct atactacaga
    33721 tgatggttat atctttaatg cttctgatat catagaggat actggtgatg cttatatcgt
    33781 tcctcatgga gatcattacc attacattcc taagaatgag ttatcagcta gcgagttggc
    33841 tgctgcagaa gccttcctat ctggtcgagg aaatctgtca aattcaagaa cctatcgccg
    33901 acaaaatagc gataacactt caagaacaaa ctgggtacct tctgtaagca atccaggaac
    33961 tacaaatact aacacaagca acaacagcaa cactaacagt caagcaagtc aaagtaatga
    34021 cattgatagt ctcttgaaac agctctacaa actgcctttg agtcaacgac atgtagaatc
    34081 tgatggcctt gtctttgatc cagcacaaat cacaagtcga acagctagag gtgttgcagt
    34141 gccacacgga gatcattacc acttcatccc ttactctcaa atgtctgaat tggaagaacg
    34201 aatcgctcgt attattcccc ttcgttatcg ttcaaaccat tgggtaccag attcaaggcc
    34261 agaacaacca agtccacaac cgactccgga acctagtcca ggcccgcaac ctgcaccaaa
    34321 tcttaaaata gactcaaatt cttctttggt tagtcagctg gtacgaaaag ttggggaagg
    34381 atatgtattc gaagaaaagg gcatctctcg ttatgtcttt gcgaaagatt taccatctga
    34441 aactgttaaa aatcttgaaa gcaagttatc aaaacaagag agtgtttcac acactttaac
    34501 tgctaaaaaa gaaaatgttg ctcctcgtga ccaagaattt tatgataaag catataatct
    34561 gttaactgag gctcataaag ccttgtttga aaataagggt cgtaattctg atttccaagc
    34621 cttagacaaa ttattagaac gcttgaatga tgaatcgact aataaagaaa aattggtaga
    34681 tgatttattg gcattcctag caccaattac ccatccagag cgacttggca aaccaaattc
    34741 tcaaattgag tatactgaag acgaagttcg tattgctcaa ttagctgata agtatacaac
    34801 gtcagatggt tacatttttg atgaacatga tataatcagt gatgaaggag atgcatatgt
    34861 aacgcctcat atgggccata gtcactggat tggaaaagat agcctttctg ataaggaaaa
    34921 agttgcagct caagcctata ctaaagaaaa aggtatccta cctccatctc cagacgcaga
    34981 tgttaaagca aatccaactg gagatagtgc agcagctatt tacaatcgtg tgaaagggga
    35041 aaaacgaatt ccactcgttc gacttccata tatggttgag catacagttg aggttaaaaa
    35101 cggtaatttg attattcctc ataaggatca ttaccataat attaaatttg cttggtttga
    35161 tgatcacaca tacaaagctc caaatggcta taccttggaa gatttgtttg cgacgattaa
    35221 gtactacgta gaacaccctg acgaacgtcc acattctaat gatggatggg gcaatgccag
    35281 tgagcatgtg ttaggcaaga aagaccacag tgaagatcca aataagaact tcaaagcgga
    35341 tgaagagcca gtagaggaaa cacctgctga gccagaagtc cctcaagtag agactgaaaa
    35401 agtagaagcc caactcaaag aagcagaagt tttgcttgcg aaagtaacgg attctagtct
    35461 gaaagccaat gcaacagaaa ctctagctgg tttacgaaat aatttgactc ttcaaattat
    35521 ggataacaat agtatcatgg cagaagcaga aaaattactt gcgttgttaa aaggaagtaa
    35581 tccttcatct gtaagtaagg aaaaaataaa ctaatgaaaa atgaaagtct cgataaagag
    35641 gctttcattt ttattatgta tatatgtaaa attcttgaca agcaatatta aaaagagtaa
    35701 actattaact agttaattaa ccggtttatt actttatagt gaatcaaata tacttaagaa
    35761 aagaggaaag aatgaaaatt aataaaaaat atctagcagg ttcagtggca gtccttgccc
    35821 taagtgtttg ttcctatgag cttggacgtt accaagctgg tcaggataag aaagagtcta
    35881 atcgagttgc ttatatagat ggtgatcagg ctggtcaaaa ggcagaaaac ttgacaccag
    35941 atgaagtcag taagagggag gggatcaacg ccgaacaaat tgttatcaag attacggatc
    36001 aaggttatgt gacctctcat ggagaccatt atcattacta taatggcaag gtcccttatg
    36061 atgccatcat cagtgaagag ctcctcatga aagatccgaa ttatcagttg aaggattcag
    36121 acattgtcaa tgaaatcaag ggtggttatg tcattaaggt aaacggtaaa tactatgttt
    36181 accttaagga tgcagctcat gcggataata ttcggacaaa agaagagatt aaacgtcaga
    36241 agcaggaacg cagtcataat cataactcaa gagcagataa tgctgttgct gcagccagag
    36301 cccaaggacg ttatacaacg gatgatgg
//
LOCUS       DAVKNL010000017        35762 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVKNL010000017 DAVKNL010000000
VERSION     DAVKNL010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35762
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.017"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            16..255
                     /locus_tag="VW411_000824"
     CDS             16..255
                     /locus_tag="VW411_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU9793584.1"
                     /translation="MPYFVSQEVYWKVRNIEAEAIRRNCERGAIFSGKIKYHEDSQFK
                     GDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            449..1783
                     /gene="trmFO"
                     /locus_tag="VW411_000825"
     CDS             449..1783
                     /gene="trmFO"
                     /locus_tag="VW411_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEU9793585.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVANHPLIEVVRDEITELPTDVITVIATGPLTSDALAEKIHALNDGAGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLSSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEAIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            1867..2604
                     /locus_tag="VW411_000826"
     CDS             1867..2604
                     /locus_tag="VW411_000826"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU9793586.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQPGNIKRVVFGENIGTTVSNNIEEKE"
     gene            2613..3170
                     /gene="frr"
                     /locus_tag="VW411_000827"
     CDS             2613..3170
                     /gene="frr"
                     /locus_tag="VW411_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEU9793587.1"
                     /translation="MANAIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDIGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKREKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            3230..4084
                     /gene="cvfB"
                     /locus_tag="VW411_000828"
     CDS             3230..4084
                     /gene="cvfB"
                     /locus_tag="VW411_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEU9793588.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            4093..4308
                     /locus_tag="VW411_000829"
     CDS             4093..4308
                     /locus_tag="VW411_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEU9793589.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEESAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            4394..5362
                     /locus_tag="VW411_000830"
     CDS             4394..5362
                     /locus_tag="VW411_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU9793590.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            5431..6245
                     /locus_tag="VW411_000831"
                     /pseudo
     CDS             5431..6245
                     /locus_tag="VW411_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558050.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS1515 family transposase"
     gene            complement(6210..6392)
                     /locus_tag="VW411_000832"
     CDS             complement(6210..6392)
                     /locus_tag="VW411_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793591.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLRPLDKTFSLYNWIFLSQKYI
                     RLVRNLVHFIIDDGTN"
     gene            6428..6928
                     /locus_tag="VW411_000833"
     CDS             6428..6928
                     /locus_tag="VW411_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9793592.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            6931..7191
                     /locus_tag="VW411_000834"
                     /pseudo
     CDS             6931..7191
                     /locus_tag="VW411_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(7558..8657)
                     /gene="ald"
                     /locus_tag="VW411_000835"
                     /pseudo
     CDS             complement(7558..8657)
                     /gene="ald"
                     /locus_tag="VW411_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003072563.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
     gene            8834..9403
                     /locus_tag="VW411_000836"
     CDS             8834..9403
                     /locus_tag="VW411_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9793593.1"
                     /translation="MESIFVKFAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKSNGQFIGTIDLHKIDSVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKANPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            9471..10121
                     /locus_tag="VW411_000837"
     CDS             9471..10121
                     /locus_tag="VW411_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEU9793594.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDSSTEKEVKKEEKEEPLEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD"
     gene            10105..12345
                     /locus_tag="VW411_000838"
     CDS             10105..12345
                     /locus_tag="VW411_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEU9793595.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCL
                     FIQFPWKSAGKVLIICGIFGFWFVFQNWQQSQASQNLADSVERVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMGNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     VFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVARRPLVFGQPNAWLLILLLI
                     SLALVYDLRKNIKGLTVLSLLITGLFFLTKYPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIKKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     SEMTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMIVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            12524..12712
                     /locus_tag="VW411_000839"
     CDS             12524..12712
                     /locus_tag="VW411_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793596.1"
                     /translation="MTVTKSYKYDWNTVWEYSTNYHDHQYAWIPSWSRYDSYSEYKVG
                     GGWNYARYEVINYYSGGY"
     gene            12745..13332
                     /locus_tag="VW411_000840"
     CDS             12745..13332
                     /locus_tag="VW411_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9793597.1"
                     /translation="MLQLTHVTLKTRQVILQDVDFTFKKGRVYGLLAINGSGKTTLFR
                     AISNLIPISSGNIAAPPSLFYYESIEWLDGNLSGMDYLRLIKNIWKSGLNLRDEIAYW
                     EMSDYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDVCDRVVTIHQGQIEEV"
     gene            13336..14520
                     /locus_tag="VW411_000841"
     CDS             13336..14520
                     /locus_tag="VW411_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9793598.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIVLALFVSVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASPGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLPKQIDNVDLNWSMGMVLLPCLIIFLLLGILFIERWGSSQKKEFFN
                     RF"
     misc_feature    14684..14815
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            14831..15361
                     /gene="infC"
                     /locus_tag="VW411_000842"
     CDS             14831..15361
                     /gene="infC"
                     /locus_tag="VW411_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEU9793599.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            15394..15594
                     /gene="rpmI"
                     /locus_tag="VW411_000843"
     CDS             15394..15594
                     /gene="rpmI"
                     /locus_tag="VW411_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU9793600.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            15646..16005
                     /gene="rplT"
                     /locus_tag="VW411_000844"
     CDS             15646..16005
                     /gene="rplT"
                     /locus_tag="VW411_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEU9793601.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            16063..16443
                     /locus_tag="VW411_000845"
     CDS             16063..16443
                     /locus_tag="VW411_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9793602.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            16693..17493
                     /locus_tag="VW411_000846"
     CDS             16693..17493
                     /locus_tag="VW411_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEU9793603.1"
                     /translation="MNPTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIDGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPENETVSQRVCEDGPVFRTGTVVL"
     gene            17504..18442
                     /locus_tag="VW411_000847"
     CDS             17504..18442
                     /locus_tag="VW411_000847"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEU9793604.1"
                     /translation="MTTNRLQVSLPGLDLKNPIIPASGCFGFGQEYAKYYDLDLLGSI
                     MIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAEKLPWLEREYPNLPIIA
                     NVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGLLIGQDPDLAYDVVKAA
                     VEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVGMRFDLKTRKPILANGT
                     GGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEMYLAGASAIGVGTANFT
                     NPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            18484..20517
                     /locus_tag="VW411_000848"
     CDS             18484..20517
                     /locus_tag="VW411_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEU9793605.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKMAEKEWVYDS
                     HSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKIAEKEWVYDSHSQAWYYFKSGGYM
                     TANEWIWDKESWFYLKSDGKIAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSD
                     GKWLGGKTTNENAAYYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAIT
                     ISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKY
                     YSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAE
                     EHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILG
                     ATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(20578..22233)
                     /gene="pavA"
                     /locus_tag="VW411_000849"
     CDS             complement(20578..22233)
                     /gene="pavA"
                     /locus_tag="VW411_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEU9793606.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTKSLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            22338..22835
                     /gene="ybeY"
                     /locus_tag="VW411_000850"
     CDS             22338..22835
                     /gene="ybeY"
                     /locus_tag="VW411_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU9793607.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRNTDRPTDVISLEYKPELEIAFDEEDLLENSELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            22816..23211
                     /locus_tag="VW411_000851"
     CDS             22816..23211
                     /locus_tag="VW411_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEU9793608.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFALTGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            23228..24127
                     /gene="era"
                     /locus_tag="VW411_000852"
     CDS             23228..24127
                     /gene="era"
                     /locus_tag="VW411_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEU9793609.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            24176..25000
                     /gene="mutM"
                     /locus_tag="VW411_000853"
     CDS             24176..25000
                     /gene="mutM"
                     /locus_tag="VW411_000853"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEU9793610.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDVYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            25000..25605
                     /locus_tag="VW411_000854"
     CDS             25000..25605
                     /locus_tag="VW411_000854"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU9793611.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKPGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPDEREWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            25592..26791
                     /locus_tag="VW411_000855"
     CDS             25592..26791
                     /locus_tag="VW411_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter PmrA"
                     /protein_id="HEU9793612.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            26791..26940
                     /gene="rpmG"
                     /locus_tag="VW411_000856"
     CDS             26791..26940
                     /gene="rpmG"
                     /locus_tag="VW411_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9793613.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            26980..27213
                     /gene="secG"
                     /locus_tag="VW411_000857"
     CDS             26980..27213
                     /gene="secG"
                     /locus_tag="VW411_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU9793614.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            27315..29669
                     /gene="rnr"
                     /locus_tag="VW411_000858"
     CDS             27315..29669
                     /gene="rnr"
                     /locus_tag="VW411_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEU9793615.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLEFGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVSSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRDSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            29632..30099
                     /gene="smpB"
                     /locus_tag="VW411_000859"
     CDS             29632..30099
                     /gene="smpB"
                     /locus_tag="VW411_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEU9793616.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQEIKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            30115..30975
                     /gene="tehB"
                     /locus_tag="VW411_000860"
     CDS             30115..30975
                     /gene="tehB"
                     /locus_tag="VW411_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEU9793617.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRISAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            31048..32001
                     /gene="coiA"
                     /locus_tag="VW411_000861"
     CDS             31048..32001
                     /gene="coiA"
                     /locus_tag="VW411_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEU9793618.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            32020..33822
                     /gene="pepF"
                     /locus_tag="VW411_000862"
     CDS             32020..33822
                     /gene="pepF"
                     /locus_tag="VW411_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU9793619.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQIEKLYAYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            33824..34537
                     /locus_tag="VW411_000863"
     CDS             33824..34537
                     /locus_tag="VW411_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEU9793620.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            34604..35545
                     /gene="prsA"
                     /locus_tag="VW411_000864"
     CDS             34604..35545
                     /gene="prsA"
                     /locus_tag="VW411_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEU9793621.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKSNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(35663..>35762)
                     /locus_tag="VW411_000865"
     CDS             complement(35663..>35762)
                     /locus_tag="VW411_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU9793622.1"
                     /translation="AIIDYIDYYNNKRIKIKLKGLSSVQYRTKSFG"
BASE COUNT        10784 a         6663 c         8041 g        10274 t
ORIGIN      
        1 attcatagaa ctactatacc atattttgtt tcgcaggaag tctattggaa agtaagaaat
       61 attgaagctg aggctattag aagaaattgt gagcgtggtg ctattttttc aggtaaaata
      121 aaatatcacg aagattcaca gtttaaagga gatcactatg ttgaatgtta tgctgtttta
      181 gataatacgg ttatagcaag agatagaata acagtcccta tcgatccgtt atgtggaaaa
      241 gattttatag agtagcatat aattgattct taactggaat actcactatc tctttacatc
      301 aagaaaatga ctaaacaggg aagtttgcct tcttcccttt ttttgttata ctagtagaag
      361 aaaaaattag aaagatttgt gggtgtcaaa cagcccagtg gggtgtttta atatggactt
      421 aggtcccacc caaagaggta ttagtgtcgt gtctcaatct tatatcaatg ttatcggtgc
      481 tggtttggca ggttctgaag cagcttacca aatcgcagag cgtggtattc cagttaaact
      541 atatgaaatg cgtggtgtca agtctacacc ccagcataaa acagacaatt ttgctgagtt
      601 ggtttgttcc aattctttgc gtggggatgc tttgacaaat gcagttggtc ttctcaagga
      661 agaaatgcgt cgcttgggtt ctgttatctt ggaatctgct gaggctacac gtgttcctgc
      721 aggtggtgcc cttgcagtgg accgtgatgg tttctctcaa atggtgaccg aaaaagttgc
      781 caaccacccc ttgattgaag tggttcgtga tgaaattaca gaattgccga cagatgttat
      841 tacggttatc gctactggtc ctttgacaag tgatgccttg gctgaaaaga ttcatgctct
      901 taatgacggt gctggttttt atttctacga tgcggcagcg cctattatcg atgtcaacac
      961 tatcgatatg agcaaggtct acctcaaatc acgttatgat aagggagaag cggcctacct
     1021 caatgcccct atgaccaagc aagaatttat ggatttccat gaagctttgg tcaatgcaga
     1081 agaagcaccg cttagttctt ttgaaaaaga aaagtacttt gaaggatgta tgcctatcga
     1141 agtcatggcc aaacgtggca ttaaaactat gctttatggc cctatgaagc cagtcggtct
     1201 tgagtaccca gacgactata caggacctcg tgatggagaa tttaaaacac cttatgcggt
     1261 tgtgcaactt cgtcaggata atgcagctgg tagcctctac aatattgttg gtttccagac
     1321 ccacctcaaa tggggagaac aaaagcgtgt cttccaaatg attccgggtc ttgaaaatgc
     1381 ggagtttgtc cgttatggtg tgatgcatcg caattcttac atggattcac caaatcttct
     1441 tgagcagact taccgttcta agaaacaacc aaatctcttc tttgctggtc aaatgacggg
     1501 tgtggaaggc tatgttgagt cggcggcttc aggcttagtt gcgggaatta acgcagctcg
     1561 tctcttcaag gaagaaagcg aggctatttt ccccgagacg acagcgattg gaagcttagc
     1621 tcattacatt acccatgccg acagcaaaca tttccaacca atgaatgtca attttgggat
     1681 catcaaggag ttggaaggcg agcgtatccg tgataagaag gctcgttatg aaaaaattgc
     1741 agagcgtgcc cttgccgact tagaggaatt tttgactgtc taattttttt gaaagaattg
     1801 ctcatgatac tataaaaatc ttagaaattg tgataaaata ggtaggatga aagaaggaga
     1861 gtgaaaatgg cgaatcccaa gtataaacgt attttaatca agttatcagg tgaagccctt
     1921 gccggtgaac gtggcgtagg gattgatatc caaacagttc aaacaatcgc aaaagagatt
     1981 caagaagttc atagcttagg tatcgaaatt gcccttgtta tcggtggagg aaatctctgg
     2041 cgtggagaac ctgcagcaga agcaggtatg gaccgtgttc aggcagatta cacaggaatg
     2101 cttgggactg ttatgaatgc tcttgtgatg gcagattcat tgcaacaagt tggggttgat
     2161 acgcgtgtac aaacagctat tgccatgcaa caagtggcag agccttatgt ccgtggacgt
     2221 gcccttcgtc accttgaaaa aggccgtatc gttatctttg gtgctggaat tggttcacct
     2281 tacttctcga cagatacaac agcggccctt cgtgcagctg aaatcgaagc agatgccatc
     2341 ctcatggcta aaaatggtgt cgatggtgtt tacaatgccg atcctaagaa agataagaca
     2401 gctgttaagt ttgaagaatt gacccaccgt gacgttatca ataaaggtct tcgtatcatg
     2461 gactcaacag cttcaaccct ctcaatggac aacgacattg acttggttgt attcaacatg
     2521 aaccaaccag gcaacatcaa acgtgtcgta tttggtgaaa atatcggaac aacagtttca
     2581 aataatatcg aagaaaagga ataagaaaga atatggctaa cgcaattatt gaaaaagcta
     2641 aagagagaat gacccagtct caccaatcac ttgctcgtga atttggtggt atccgtgctg
     2701 gtcgtgccaa tgcaagcttg cttgaccgtg tacatgtaga atactatgga gtcgaaactc
     2761 ctcttaacca aatcgcttca attacgattc cagaagcgcg tgttttgttg gtaacaccat
     2821 ttgacaagtc ttcattgaaa gacatcgaac gtgccttgaa cgcttctgat attggtatca
     2881 caccggctaa tgacggttct gtgattcgct tggttatccc agctcttaca gaagaaactc
     2941 gtcgtgacct tgctaaagaa gtgaagaagg tcggcgaaaa tgctaaagtg gctgtccgca
     3001 atatccgtcg cgatgctatg gacgaagcta agaaacgaga aaaagcaaaa gaaatcactg
     3061 aagacgaatt gaagactctt gaaaaagaca ttcaaaaagt aacagacgat gctgttaaac
     3121 acatcgacga catgactgct aacaaagaga aagaactttt ggaagtctaa aaataaacag
     3181 aaaaactcag ttggcattgc tggctgagtt ttattcgaaa gaaggaaata tgaatacaaa
     3241 tcttgcaagt tttatcgttg gactgatcat cgatgaaaac gaccgttttt actttgtgca
     3301 aaaggatggt caaacctatg ctcttgctaa ggaagaaggc caacatacag taggggatac
     3361 ggtcaaaggt tttgcataca cggatatgaa gcaaaaactc cgcctgacaa ccttagaagt
     3421 gactgccact caggaccaat ttggttgggg acgtgtcaca gaggttcgta aggacttggg
     3481 tgtctttgtg gatacaggcc ttcctgacaa ggaaatcgtt gtgtcactcg atattctccc
     3541 tgagctcaag gaactctggc ctaagaaggg cgaccaactc tacatccgtc ttgaagtgga
     3601 taagaaagac cgtatctggg gcctcttggc ttatcaagaa gacttccaac gtcttgctcg
     3661 tcctgcctac aacaacatgc agaaccaaaa ctggccagcc attgtttacc gtctcaagct
     3721 gtcaggaact tttgtttacc taccagaaaa taatatgctt ggttttattc atcctagcga
     3781 gcgttacgca gagccacgtt tggggcaagt attagatgcg cgcgttattg gtttccgtga
     3841 agtggaccgc actctgaacc tctccctcaa accacgctcc tttgaaatgt tggaaaacga
     3901 tgctcagatg attttgactt atttggaaag caatggcggt ttcatgacct taaatgacaa
     3961 gtcatctcca gacgacatca aggcaacctt tggcatttct aaaggtcagt tcaagaaagc
     4021 tttaggtggt cttatgaagg ctggtaaaat caagcaggac cagtttggga cagagttgat
     4081 ttagggaggc ttatgagaaa atcattttac acttggctca tgaccgagcg caatcctaaa
     4141 agtaacagtc ccaaagcaat tttggcagac ctcgcttttg aagagtcagc ctttccaaaa
     4201 cacacagatg attttgatga ggtcagtcgc tttttggagg agcatgccag tttctctttt
     4261 aacctaggag attttgacag catttggcag gaatatctag aacactagca tttattcatt
     4321 gggtttgggc tagtaatttc tccatccctc tgctataata aaaagaaata aaaggattag
     4381 agaggttctt tatttgaagg aacattcaat agacattcaa ctgagtcatc cagatgacct
     4441 gtttcatctt tttggttcca atgaacgcca tcttcgtttg atggaagaag agcttgatgt
     4501 tgtgattcat gctcgtacgg agattgtcca ggttttggga gaagagtctg cctgtgagga
     4561 agcccgtcaa gttattcagg ctttgatggt cttggtaaat cgtgggatga ccgttggtac
     4621 gccagatgta gtcactgcga ttagcatggt caaaaatgat gaaattgaca agtttgtcgc
     4681 cctttacgaa gaagaaatta tcaaggataa tactgggaaa cctatccgtg tcaaaaccct
     4741 agggcaaaag ctttatgtgg acagtgtcaa acagcatgat gtgacctttg gaattgggcc
     4801 agcaggtaca gggaagacct tccttgcagt gaccttggca gtgactgccc ttaaacgtgg
     4861 gcaagtcaag cgaattatcc taactcgtcc agcggtggaa gcgggagaga gtcttggatt
     4921 tcttccgggt gatcttaagg agaaggtgga tccttacctt cgtcctgttt acgatgcctt
     4981 gtatcaaatt cttgggaaag accaaacgac tcgtctcatg gagcgtgaaa ttatcgaaat
     5041 tgcgcccctt gcctatatgc gtggccggac cttggatgat gcctttgtca ttctcgatga
     5101 ggcgcaaaac acgaccatca tgcagatgaa gatgttcttg acgcgtttag gttttcattc
     5161 taagatgatt gtcaatggag atattagtca gattgacctg ccacgtaatg tcaagtccgg
     5221 tttgattgat gctcaagaga aactcaagaa catccatcag attgactttg ttcatttttc
     5281 agccaaggat gtggttcgcc atcctgttgt cgctcagatt atccgagcct atgaatattc
     5341 tactgaagtt gcacacgact gattttgagg aagttcgcct gcaaaagaat agacttgttc
     5401 ggtaactgta aaaagtgtta tactattttt atggaaacag tatacgacaa agcacaaaaa
     5461 cttaactcaa aaaacttcaa actattgatt ggtgtcaaaa aggaaacctt tcaactcatg
     5521 ctagaacacc tgaattcagc ctatcagatt cagcaccgaa aaggtggacg tccacgtagt
     5581 ctgcccatgg aagaccagct cattatgacc ctccgttact tgcgatatta tcccactcag
     5641 cgtctgctgg cctttgattt tggcgtcggt gtagctacgg taaatgccat catcacttgg
     5701 gtggaggata cacttcgtgc gtcaggtagc tttgatttgg accatttaga agccccgagt
     5761 gctgctgtgg ctattgacgt gaccgaaagt ccgattcagc gtccaaacaa aaccaaagca
     5821 aaaattattc tggtaaaaag aaacgacaca ccttaaaaac tcaaattatg ctggatttga
     5881 cgacacataa agtctgtcaa atggcctttt ctgacggaca tacgcatgat tttactctct
     5941 tcaaagaaag tattggacaa agtttgcctg aaacgacgct tgcctttgtt gacctaggtt
     6001 atttaggcat cttgaaattt catgagaata ctttcattcc tgctaaaaat tccaaaaatc
     6061 gccgcctgag tgaggatgat aagcagttaa ataaagagat gtcagcgata cgaattgaaa
     6121 ttgaacattt taacgctaaa ttcaagacct tccaaatcat gtcagtccct tatcgtaacc
     6181 gcagaaaacg tttcgagtta cgggcggaat taatttgtgc catcatcaat tatgaagtga
     6241 actagattcc gaacaagtct aatatacttt tgagagagga aaatccagtt gtataggcta
     6301 aaggttttat ccaaaggtct gagacaacga ttaggcacga tggaaagaac ttttatgtgg
     6361 ctgatgacga tcagtgcatc ttcctgtgtc ataatcacag ggcacaagaa agtaggaatt
     6421 tgaaaagatg attgaccaac tatctaagta ttacagttgt aggatactaa ctgaaaagga
     6481 tattccaagt attttatctt tatatgaaag taatcctctg tattttcagc attgtccacc
     6541 agagccaaat tttgcaactg taaaagagga catgctttgt ctacctgaag gtaaagctaa
     6601 ggctgataag ttttttgttg gattttggaa tggatctgac cttgtggctg ttatggattt
     6661 tgtctatgca tatcctgatg aggagactgt ttttattggt ttgtttatgg ttgatcaagc
     6721 ctatcagaga aaagggattg gtagtcatat tgtgacagaa gcactagctt attttgctaa
     6781 gaactttcga aaggcacgtt tggcttatgt taagggaaat ccgcaatctc agcatttttg
     6841 ggaaaagcag ggctttaaat caattggatg cgaggttaag caagaactct atacggttgt
     6901 tatcgctgaa cagagcctag aagattagaa atggcatcaa gtaagaacta tttggaattt
     6961 gttttggaac aattatcagg attagatgat gtgacttacc gttccatgat gggggagtat
     7021 attctttact tccgcggcaa gattattggc ggcatttatg acgatcgctt tttagttaaa
     7081 cccgtgcaag cagtcttaga taagattgac caatcttctt ttgagtttcc atacaaaggt
     7141 gccaaagaaa tgatttgagt ggaagaactt gataataaga tgtttctata agacctaatt
     7201 ttagctatgt ataaccaact gccaacgccc aaacctaaaa agaaaaagca agggtgaacg
     7261 aagtaaaaaa gaagtctgct aaggccctgt ctttgcacgg gtaaaatttt atatataaaa
     7321 agaagctggg actaaagagc tcagcttcct ttggtttata taattgtcat tacaagacga
     7381 agtggttggg cgaaactctg ttgactttat tcaatttaga gtttcttatg cacaattgag
     7441 tctggaacga aagtctccag ttgcaaagta tacagtacaa taaaccaacg atgtaatagc
     7501 tgatgacaca aagcacagtg ggtaggactt gcgaagtcac ccttttcttt tcaaaattta
     7561 tactaaatca ttgatatcag tgtagtcacg attaagtcct tgagcaactg gtaggttagt
     7621 caagtaacct tgataagtag tcacaccttg acgcaagcct tcatcttcag agattgcttg
     7681 tgcgaatcct ttgccagcca aagcttcgat ataaggaaga gtgacattgg ttagggcgat
     7741 ggttgaagtg cgagcaaccg caccagggat attggcaacg gcatagtgga gaacaccgtg
     7801 tttttcatag acgggttcat cgtgcgttgt cacacggtca gctgtttcga taacgccacc
     7861 ttggtcaaca gcaacgtcaa cgatacagag cctggacgca tttgtttgac catctcatct
     7921 gtcaccaatt ccggtgcttt tgcaccaggg atgagaatgg ctccaatcac cacatcagca
     7981 tctctcacac ttgcttcaat gttgaatgaa ttagacataa gagtttgaat ttgacttcca
     8041 aagacttctt ctagaactga gagacgcttg gaactaatat ctaaaatagt cacttgagca
     8101 ccaagaccaa gggcgatgcg ggcagcatgt gtaccgacga caccaccacc gatgatagtt
     8161 acttttcctt ttggaacacc tggtacacca ccaagtagaa caccagagcc accagcttgc
     8221 ttagtaagga agtgagctcc gatttgaaca gccatacgac ctgcaacctc actcatagga
     8281 acgaggagcg gtagttgtcc ttgattgtca cgaacagttt cagttgtttt tgctgttaac
     8341 atagcatctg ctaattctgg agcagcggcc atgtgcaagt aggtgaagag aagaagatcg
     8401 tcgcgcaagt aaccgtattc agaacttaaa gattctttta ctttcacaac caactctgct
     8461 gcccaagctt caccagcagt agcgacaatc tcagctcctt gcttttgata gtcagcatca
     8521 gtaaagccag aaccgagacc agcatttgtt tcgataagga cacgatgacc acgactaact
     8581 aagctatgaa cacctgcagg tgtgagggcg acacggtttt cgttattttt aatttctttt
     8641 gggattccga ttaacattga gataacctac ctttcaattg acggtcttgt tttggttgtc
     8701 acattccagt tcataaatca aaaatgtgac ggtttcattg tatatgaaac cgcttcaaaa
     8761 atcaagaaaa acttgtcatc caaatttttt tatgctagac tagtgaaaat caagctctaa
     8821 tggagggaaa agtatggaat caatatttgt gaaatttgcc cagtatccgt ctatagaaac
     8881 ggagcgttta ttgctcagac ctgtaacttt ggatgatgcg gaagcaatgt ttgactatgc
     8941 ctcggacaag ggtaatacac gttacacttt tccaaccaat caaagcttgg aagaaaccaa
     9001 gaataacatt gctcagttct acttggctaa tcccttggga cgttggggaa tagaactaaa
     9061 aagcaatggt cagtttattg gaaccattga cttgcacaag attgattctg ttcttaagaa
     9121 ggcagctatt ggctacatta tcaataaaaa gtattggaat caaggattaa cgacagaagc
     9181 caatcgtgct gtgattgagc tagcttttga gaagataggg atgaataagt tgactgccct
     9241 tcacgataag gctaatcccg cgtcaggaaa ggtcatggag aaatcaggca tgcgtttttc
     9301 ccatgcagaa ccatatgctt gtatggacca gcatgaaaaa ggccgaatcg tgacaagagt
     9361 tcattatgtc ttgaccaagg aagactattt tgcaaataaa taagcagttg aaaagaaatt
     9421 tttcgactgt tttttcttcc tcttacgaat aatctaagag aggagaaaat atggaagcaa
     9481 ttatcgagaa aatcaaagag tataaaatca tcgtcatctg tactggtctg ggcttgcttg
     9541 taggaggatt tttcctgcta aaaccagctc cacaaacacc tgtcaaagag acgaatttgc
     9601 aggctgaagt tgcagctgtt tccaaggact catcgaccga aaaggaagtg aagaaggaag
     9661 aaaaggaaga accccttgaa caagatctaa tcacagtaga tgtcaaaggt gctgtcaaat
     9721 cgccagggat ttatgacttg cctgtaggta gtcgagtcaa tgatgctgtt cagaaggctg
     9781 gtggcttgac agagcaagca gacagcaagt cgctcaatct agctcagaaa gttagtgatg
     9841 aggctctggt ttacgttcct actaagggag aagaagcagt tagtcaacag actggttcgg
     9901 ggacagcttc ttcaacaagc aaggaaaaga aggtcaatct caacaaggcc agtctggaag
     9961 aactcaagca ggtcaaggga ctgggaggaa aacgagctca ggacattatt gaccatcgtg
    10021 aggcaaatgg caagttcaag tcagtagacg agctcaagaa ggtctctggc attggtggca
    10081 aaacaataga aaagcttaaa gactatgtta cagtggatta agaatttctc tattccccta
    10141 atttacctga gttttctatt actttggctt tattacgcta ttttctcagc atcttatctt
    10201 gctttgttgg gctttgtttt tctgctagtc tgtctcttta tccaatttcc gtggaaatct
    10261 gctggtaaag ttctaataat ttgcggaatc tttggatttt ggtttgtttt tcaaaattgg
    10321 caacagagtc aagcgagtca aaatctggcg gattctgttg aaagggtacg gattttgcct
    10381 gatactatta aggttaatgg tgatagtcta tcctttcgtg gcaagtctaa cggtcgtgct
    10441 ttccaagtct attataaact ccagtccgag gaggagaaag aagcctttca agctttaact
    10501 gacctgcatg agataggact agaagggaag ctttcggagc cagaagggca gagaaatttt
    10561 ggtggcttta attaccaagc ctatctgaag actcagggaa tttaccagac tctcaatatc
    10621 aaaacaatcc agtcacttca aaagattggc agttgggata taggagaaaa cttgtccagt
    10681 ttacgtcgaa aggctgtggt ttggattaag acgcactttc cagaccctat gggcaattac
    10741 atgacaggac tcttgctggg acatctggac accgactttg aggagatgaa tgagctttat
    10801 tccagtctag gaattatcca cctctttgcc ctatctggca tgcaggtagg ttttttcatg
    10861 aatggattta agaaacttct cttgcgattg ggcttgaccc aagaaaagtt gaaatggctg
    10921 acttatccct tttcccttat ctatgcggga ctaactggat tttcagcatc ggttattcgc
    10981 agtctcttgc aaaagctact ggctcaacat ggggttaagg gcttggataa ttttgccttg
    11041 acggtgcttg tcctctttat tgtcatgcca aactttttct tgacagcagg aggagtcttg
    11101 tcctgcgctt atgcttttat cctgaccatg accagcaaag aaggggaggg gctcaaggct
    11161 gttactagtg aaagtctagt catctccttg ggcatattgc ccattctatc cttctatttt
    11221 gcggaatttc aaccttggtc tatccttttg acctttgtct tttcctttct ttttgacttg
    11281 gtcttcttac cgctcttgtc tatcttattt gtcctttcct ttctctatcc agtcattcag
    11341 ctgaacttta tctttgaatg gttagagggc attattcgct tggtctcgca ggtggcaagg
    11401 agaccacttg tctttggtca acccaacgca tggcttttaa tcttattgtt aatttccttg
    11461 gctttggtct atgatttgag gaaaaacatt aaaggattaa cagtattgag tttattgatt
    11521 acaggtctct ttttccttac caagtatcca ctggaaaatg aaatcaccat gctggatgtg
    11581 gggcaaggag aaagtatttt cctacgggat gtaactggga aaaccattct catagatgta
    11641 ggtggtaagg cagaatctta taagaaaatc aaaaaatggc aagaaaagat gacgaccagc
    11701 aatgcccagc gaaccttgat tccctatctc aaaagtcgag gagtagctaa gattgaccag
    11761 ctaattttga ctaacacgga caaggagcat gttggagatt tgtcagagat gaccaaggct
    11821 ttccatgtag gggagattct agtatcaaaa gacagtctga aacagaagga atttgtggca
    11881 gaactacagg cgactcaaac aaaggtgcgt agtatgatag taggggagaa cttgcccatt
    11941 tttggaagtc agttagaagt tctatctcca aggaaaatgg gagatggagg acacgatgat
    12001 accctagttc tgtatgggaa attcttggat aagcaatttc tcttcacggg aaatttggag
    12061 gagaaaggag agaaggactt gctgaagcac tatccagact tgaaagtaaa tgttttgaaa
    12121 gctagccaac atggcaataa aaaatcatca agtccagcct ttctagaaaa actcaaacca
    12181 gagcttactc ttatctcagt tggaaagagc aatcgaatga aactccccca tcaggaaaca
    12241 ttgacacgac tggaaggtat caatagcaaa gtttatcgaa ctgaccagca aggagctata
    12301 cgttttaagg ggttggatag ttggaaaatc gaaagtgttc gataggaagg ataaatgttg
    12361 tagattagtg aaataaacta aaaatttgtt gcataataat gataaaaatg gtataatgaa
    12421 aacgtattca atattgagga tataaaatca ttaaaaatca gcaaaagttg ttttattagt
    12481 tagtttataa tctattggtc ttcttcagtc cagtgtatct gctgtgacag tcactaaaag
    12541 ttacaagtat gattggaata cggtttggga atatagtacc aactatcacg accatcagta
    12601 tgcttggatt ccgtcatggt ctcgttatga cagctattct gagtataaag ttggcggagg
    12661 ctggaactac gctcgttatg aggtcataaa ctattacagc ggaggctatt aattcttaaa
    12721 gagtgagaaa aaggagggct agatatgttg cagcttactc atgtgacctt aaaaacgcga
    12781 caagtcatct tgcaagatgt ggatttcacc tttaaaaagg gtagggttta tggtcttctt
    12841 gctatcaatg gctctggaaa gacgaccctg ttccgtgcca ttagcaattt aattcccata
    12901 agtagtggaa atatcgcagc ccctccttct ttattttatt atgagagtat tgaatggctg
    12961 gatggaaact taagtgggat ggactacctt cgtcttatca aaaacatctg gaagtcaggt
    13021 ctgaacttga gggatgaaat cgcctattgg gaaatgtctg actatatcag tcttcccatt
    13081 cgcaagtatt ccttaggcat gaagcaacgc ttggtgattg ccatgtattt cctcagtcag
    13141 gccaaatgct ggctcatgga tgagattaca aatggcttag atgagtatta tcgacagaag
    13201 ttttttgata ggctagcaca aatcgataga caagaacagc tggttctttt aagttcccac
    13261 tataaggaag agttggttga tgtctgcgat agagtagtaa ccattcatca ggggcagata
    13321 gaagaggttt agtttatgaa agatgttagt ctatttttat tgaaaaaagt tttcaaaagc
    13381 cgcttaaact ggattgtctt agctttattt gtatctgtac tcggtgttac cttttattta
    13441 aatagtcaga ctgcaaactc acacagcttg gagagcaggt tggaaagtcg cattgcagcc
    13501 aacgagaggg ctatcaatga aaatgaagag aaactctccc aaatgtctga taccagctcg
    13561 gaggaatacc agtttgctaa aaataattta gacgtgcaaa aaaatctttt gacgcgaaag
    13621 acagaaattc tgactttatt aaaagaaggg cgctggaaag aagcctacta tttgcagtgg
    13681 caagatgaag agaagaatta tgaatttgta tcaaatgacc cgactgctag ccctggctta
    13741 aaaatggggg ttgaccgcga acggaagatt taccaagccc tgtatccctt gaacataaaa
    13801 gcacatactt tggagtttcc gacccacggg attgatcaga ttgtctggat tttagaggtt
    13861 atcatcccaa gtttgtttgt ggttgctatt atttttatgc taacacaact atttgcagaa
    13921 agatatcaaa atcatctgga cacagctcac ttatatcctg tttcaaaagt gacatttgca
    13981 atatcctctc ttggagttgg agtgggatat gtaactgtgc tgtttatcgg aatctgtggc
    14041 ttttcttttc tagtgggaag tctgataagt ggttttggac agttagatta tccctaccca
    14101 atttatagct tagtgaatca agaagtaact attgggaaaa tacaagatgt attatttcct
    14161 ggcttgctct tagctttctt agcctttatc gtcattgtgg aagttgtgta cttgattgct
    14221 tactttttca agcaaaaaat gcctgtcctc tttctttcac tcattgggat tgttggctta
    14281 ttgtttggta tccaaaccat tcagcctctt caaaggattg cacatctgat tccctttact
    14341 tacttgcgtt cagtggagat tttatctgga agattaccta agcagattga taatgtcgat
    14401 ctaaattgga gcatgggaat ggtcttactt ccttgcctga ttatcttttt gctattggga
    14461 attctattta ttgaaagatg gggaagttca cagaaaaaag aattttttaa tagattctag
    14521 ctttcctata ggtagggaaa ataagtaaaa actaacatag agagggaatc aacttgattc
    14581 tctctttttg attcgaaaac caaaccaaaa tacaaacaca aacttttcaa aaaataactt
    14641 tttatcttga caagagctag aaaacttggt atcatataaa agttgagaaa agcagaagtg
    14701 agagcttctc gccttgtgac attaagttgc ctggccctac ggatgaaaag tttcgaagaa
    14761 acgctatcat aacgtgcggg cttgtatatt tacaagtccg ctattgtttt tctctaataa
    14821 aacaaaagag gtgaaaacca tagcaaagca agacttattc atcaatgatg agattcgtgt
    14881 acgtgaagtt cgcttgattg gtcttgaagg agaacagcta ggtatcaagc cactcagtga
    14941 agcgcaagct ttggctgata acgctaatgt tgacctagta ttgattcaac cccaagccaa
    15001 accgcctgtt gcaaaaatta tggactacgg taagttcaaa tttgagtacc agaagaagca
    15061 aaaagaacaa cgtaaaaaac aaagcgttgt tactgtgaaa gaagttcgtc taagtccaac
    15121 aattgacaag ggtgactttg atacaaaact tcgtaatgca cgtaagttcc ttgaaaaagg
    15181 aaataaagtt aaggtatcta ttcgctttaa gggtcgtatg attacccata aagagattgg
    15241 tgcaaaagtt ttagccgagt ttgctgaagc aactcaagat attgccatca tcgaacaacg
    15301 tgctaaaatg gatggacgtc aaatgttcat gcaattggcg ccagcgactg acaaaaaata
    15361 attgtcagaa agttaaataa aggagaaaaa atcatgccaa aacaaaaaac acaccgcgca
    15421 tcagctaaac gtttcaaacg tacaggttct ggtggactta aacgtttccg tgcttacact
    15481 tctcaccgtt tccacggaaa aactaagaaa caacgtcgtc atcttcgtaa agcatctatg
    15541 gtgcattcag gagattacaa acgtatcaaa gcaatgctta ctcgcttgaa ataatagctt
    15601 gactgtaagt aaacaattct aggaaatatt tggaggaaat aaaacatggc acgtgttaaa
    15661 ggtggcgttg tatcacgcaa acgtcgtaaa cgtattctta aattagcaaa aggttactat
    15721 ggagctaaac acatcttgtt ccgtactgca aaagaacaag taatgaactc ttactactat
    15781 gcataccgtg accgtcgtca gaaaaaacgt gacttccgca aattgtggat tactcgtatc
    15841 aatgcggcag ctcgtatgaa cggactttca tactcacaat tgatgcatgg tttgaaattg
    15901 gctgagatcg aagttaaccg taaaatgctt gctgacttgg ctgttaacga tgcagtagct
    15961 ttcacagctc ttgcagatgc agctaaagca aaacttggta aataacaact gatgagactg
    16021 gaacaggatt gtccagtctt ttttaaaata aaggagaaag atatggcttc aaaaatgcta
    16081 cacacttgct tacgagtaga aaatcttgaa aaatcaatcg cattttatca agatgctttt
    16141 ggttttaaag aattgcgtcg cagagatttt ccagaccatg ccttcacgat tgtctatcta
    16201 ggtcttgagg gcgatgacta tgagttggag ttgacttata actacgatca cggtccgtat
    16261 gtggttggtg atgggtttgc ccatatcgcc ctcagtacac ctgatcttga ggcacttcat
    16321 caagaacaca gtgcaaaagg ctatgaagtg actgagccaa atggtctacc aggaactaca
    16381 ccaaactatt actttgtcaa agaccctgat ggatataagg tcgaagtcat tcgtgaaaaa
    16441 taatttcgtg caagttaagt agaatattcg ttttctactt gacttttttt agctgaaaga
    16501 gtatactaat aaaaattcaa cctttaaggg gagtccagag agactcacaa ggtgtcagat
    16561 aaaagaatgg tgcaattttc tagaggagac tttttgagtg tgctctcttg tgttgtacga
    16621 ttttaactga ggccttgcac tagcaaggtc ttttctttat ctggtcccct taaaatttaa
    16681 ggaggaaaag ttatgaatcc cacatgtaag aagcgtttgg gtgtcattcg gttggaaacc
    16741 atgaaggtgg ttgcacaaga ggaaatcgcg ccagcaatct ttgaattagt cctagaagga
    16801 gaaatggttg aagccatgcg agcaggccaa tttcttcatc tgcgtgtacc ggacgatgcc
    16861 catctcttac gtcgtcctat ttcaatttcg tctattgaca aggcaaacaa gcagtgtcac
    16921 ctcatttatc ggattgacgg agctgggact gcaatttttt caaccttaag tcagggagac
    16981 actcttgatg tgatggggcc tcagggaaat ggttttgact tgtctgacct tgatgagcag
    17041 aatcaggttc tccttgttgg tggtgggatt ggtgttccac ccttgcttga ggtggccaag
    17101 gaattgcatg aacgtggagt gaaagtagtg acagtcctcg gttttgctaa taaggatgct
    17161 gttattttga aaacggaatt ggctcagtat ggtcaggtct ttgtaacgac agatgatggt
    17221 tcttatggca tcaagggaaa tgtttccgtt gttatcaatg atttagacag tcagtttgat
    17281 gctgtttact cgtgtggggc tccaggaatg atgaagtata tcaatcaaac ctttgatgat
    17341 cacccaagag cctatttatc tctggaatct cgtatggctt gtgggatggg agcttgctat
    17401 gcctgtgttc taaaagtacc agaaaacgag acggtcagcc aacgcgtctg tgaagatggt
    17461 cctgttttcc gcacaggaac agttgtatta taaggagaaa attatgacta caaatcgatt
    17521 acaagtttct ctacctggtt tggatttgaa aaatccgatt attccagcat caggctgttt
    17581 tggctttgga caagagtatg ccaagtacta tgatttagac cttttaggtt ctattatgat
    17641 caaggcgaca acccttgaac cacgttttgg gaatccaact ccaagagtgg cagagacgcc
    17701 tgctggtatg ctcaatgcaa ttggcttgca aaatcctggt ttagaggttg ttttggctga
    17761 aaagctacct tggctggaaa gagaatatcc aaatcttcct attattgcca atgtagctgg
    17821 tttttcaaaa caagagtatg cagctgtttc tcatgggatt tccaaggcaa ctaatgtaaa
    17881 agctatcgag ctcaatattt cttgtcccaa tgttgaccac tgtaatcatg gacttttgat
    17941 tggtcaagat ccagatttgg cttatgatgt ggtgaaagca gctgtggaag cctcagaagt
    18001 gccagtttat gtcaaattaa ccccgagtgt gaccgatatc gttactgtcg caaaagctgc
    18061 agaagatgcg ggagcaagtg gcttgaccat gatcaatact ctggttggaa tgcgctttga
    18121 cctcaaaact agaaaaccaa tcttggccaa tggaacaggt ggaatgtctg gtccagcagt
    18181 ctttccagta gccctcaaac tcatccgcca agttgcccaa acaacagacc tgcctatcat
    18241 tggaatggga ggagtggatt cggctgaagc tgccctagaa atgtatctgg ctggggcatc
    18301 tgctatcgga gttggaacag ctaactttac caatccttat gcctgccctg acatcatcga
    18361 aaatttacca aaagtcatgg ataaatacgg tattagcagt ctggaagaac tccgtcagga
    18421 agtaaaagag tctctgaggt aaactgcaat caatctgttc ttgatttttt attagtttgt
    18481 aatatgaatt taggagaatt ttggtacaat aaaataaata agaacagagg aagaaggtta
    18541 atgaagaaag taagatttat ttttttagct ctgctatttt tcttagctag tccagagggt
    18601 gcaatggcta gtgatggtac ttggcaagga aaacagtatc tgaaagaaga tggcagtcaa
    18661 gcagcaaatg agtgggtttt tgatactcat tatcaatctt ggttctatat aaaagcagat
    18721 gctaactatg ctgaaaatga atggctaaag caaggtgacg actattttta cctcaaatct
    18781 ggtggctata tggccaaatc agaatgggta gaagacaagg gagcctttta ttatcttgac
    18841 caagatggaa agatgaaaag aaatgcttgg gtaggaactt cctatgttgg tgcaacaggt
    18901 gccaaagtaa tagaagactg ggtctatgat tctcaatacg atgcttggtt ttatatcaaa
    18961 gcagatggac agcacgcaga gaaagaatgg ctccaaatta aagggaagga ctattatttc
    19021 aaatccggtg gttatctact gacaagtcag tggattaatc aagcttatgt gaatgctagt
    19081 ggtgccaaag tacagcaagg ttggcttttt gacaaacaat accaatcttg gttttacatc
    19141 aaagaaaatg gaaactatgc tgataaagaa tggattttcg agaatggtca ctattattat
    19201 ctaaaatccg gtggctacat ggcagccaat gaatggattt gggataagga atcttggttt
    19261 tatctcaaat ttgatgggaa aatggctgaa aaagaatggg tctacgattc tcatagtcaa
    19321 gcttggtact acttcaaatc cggtggttac atgacagcca atgaatggat ttgggataag
    19381 gaatcttggt tttatctcaa atctgatggg aaaatagctg aaaaagaatg ggtctacgat
    19441 tctcatagtc aagcttggta ctacttcaaa tccggtggtt acatgacagc caatgaatgg
    19501 atttgggata aggaatcttg gttttacctc aaatctgatg ggaaaatagc tgaaaaagaa
    19561 tgggtctacg attctcatag tcaagcttgg tactacttca aatctggtgg ctacatggcg
    19621 aaaaatgaga cagtagatgg ttatcagctt ggaagcgatg gtaaatggct tggaggaaaa
    19681 actacaaatg aaaatgctgc ttactatcaa gtagtgcctg ttacagccaa tgtttatgat
    19741 tcagatggtg aaaagctttc ctatatatcg caaggtagtg tcgtatggct agataaggat
    19801 agaaaaagtg atgacaagcg cttggctatt actatttctg gtttgtcagg ctatatgaaa
    19861 acagaagatt tacaagcgct agatgctagt aaggacttta tcccttatta tgagagtgat
    19921 ggccaccgtt tttatcacta tgtggctcag aatgctagta tcccagtagc ttctcatctt
    19981 tctgatatgg aagtaggcaa gaaatattat tcggcagatg gcctgcattt tgatggtttt
    20041 aagcttgaga atcccttcct tttcaaagat ttaacagagg ctacaaacta cagtgctgaa
    20101 gaattggata aggtatttag tttgctaaac attaacaata gccttttgga gaacaagggc
    20161 gctactttta aggaagccga agaacattac catatcaatg ctctttatct ccttgcccat
    20221 agtgccctag aaagtaactg gggaagaagt aaaattgcca aagataagaa taatttcttt
    20281 ggcattacag cctatgatac gaccccttac ctttctgcta agacatttga tgatgtggat
    20341 aagggaattt taggtgcaac caagtggatt aaggaaaatt atatcgatag gggaagaact
    20401 ttccttggaa acaaggcttc tggtatgaat gtggaatatg cttcagaccc ttattggggc
    20461 gaaaaaattg ctagtgtgat gatgaaaatc aatgagaagc taggtggcaa agattagtac
    20521 tataagtgaa tatgatttga gtgaatagta agttaaaaat cctgatttca agtaaaatca
    20581 ggattttttc atggatgcaa tttttttgga gtctggtgtg acgcggaggg tcttttgtcc
    20641 tgtgtaagtg acaaagccgg gttttccacc agttggttta ttgagttttt tgacttcaat
    20701 catatctacc tgcaccagat tcgacaggcg cccttgagag aagtaggcag ctaactctgc
    20761 tgcgtctgtc ttgactgcat cagatgggtc aagatttcct gagatgacaa catggcttcc
    20821 aggaatgtcc ttagcatgga accaaagttc ctccttgcgg gccattttaa aggtcaattc
    20881 ctcattttga agattgtttc gtccgacata gatgatggtt ttgccatcgc ttgctagata
    20941 ttgttctagt tttttgcgtt tctggatttt ctcccgttgt cttctgcgga taaaacctgt
    21001 ttgaatcaat tcttcacgga tttcagcgat ttcttccagt ccagcttggt tgaggacggt
    21061 ttctacactt tccagataga gaatagtggc tttggtttct tcaatcaaat cagtcaagta
    21121 tttgacagct tctttgagtt tctgataccg tttaaaatag cgttgggcat tctggttggg
    21181 agtcagagcc ttatcaagcg caatcatgat aggttggttg gtatagtagt tgtctaggat
    21241 aacctggtct tggtcgttag gcacttggtg gaggaaggtt gtcagcaatt ctcctttttg
    21301 acgaaattct tcagcgttgt ctgtcgccag taactctttt tcctgttttt tgagtttgtg
    21361 tcggtttttc tgaagttcat tttcaacacg acgaatcagt tcactggcct gctgtttgac
    21421 gcggtcgcgc tcagccttat ccttatagta ggtgtccaac aaatcagaaa gatttgcaaa
    21481 aggctctccc acctgatttg caaaaggaac tggactgaag gaagtctcag tcaagcatgg
    21541 cttggtttct tgattgaaaa aatttcggaa agcggaaagt ttttcactaa ccagtatcct
    21601 ttccaattca tttgccgtat cgcgtcccag accttgaaag aggctttgaa gattttttgc
    21661 tgttagttct tgggtttgca ggatttcaaa gagcttttca tccttgatag taaaaggatt
    21721 gagagatttt gtacttggcg gagcgatata ggtcgatcct ggaagtaagg tgcggtagct
    21781 attttgtgaa aagccgacgt gtttgataac ttcgaggatt ttatgactgc ttttatcgac
    21841 cagtagaata ttactgtgtt tccccataat ttcgataatc aaggtagcct ggatatggtc
    21901 tccaatctcg tttttattgg aaactgtaat ttccacaata cggtcatttt ccacttgctc
    21961 aatcgactca atcagggccc cctgcaaata ctttctcaaa accatgataa aggtagaagg
    22021 ttgagctgga ttttcaaaag tcgtttgggt cagctgaatg cgtccaaaaa ctggatgggc
    22081 agaaaggagc aggcgatggc tttggcgatt gctgcggatt tgcaagacca actcttgttc
    22141 aaaaggctga ttgattttct ggatgcgacc attcactaat tcgcttcgca attcctcaac
    22201 tatgtggtgt aaaaaaaatc cgtcaaatga catcgttctc tccttgtgat tgtattccat
    22261 agtattatat caaaaaggta gaataaaatc atggaaatgt ggtataataa agccaagtaa
    22321 agagaaacga gaagcacatg tatattgaaa tggtagatga aactggtcaa gtttcaaaag
    22381 aaatgttgca acaaacccaa gaaattttgg aatttgcagc ccaaaaatta ggaaaagaag
    22441 acaaggagat ggcagtcact tttgtgacca atgagcgtag tcatgaactt aatctggagt
    22501 accgtaacac cgaccgtccg acagatgtca tcagccttga gtataaacca gaattggaaa
    22561 ttgcctttga cgaagaggat ttgcttgaaa attcagaatt ggcagagatg atgtctgagt
    22621 ttgatgccta tattggggaa ttgttcatct ctatcgataa ggctcatgag caggccgaag
    22681 aatatggtca cagctttgag cgtgagatgg gcttcttggc agtacacggc tttttacata
    22741 ttaacggcta tgatcactac actccggaag aagaagcgga gatgttcggt ttacaagaag
    22801 aaattttgac agcctatgga ctcacaagac aataaacgaa aatggaaaaa tcgtgacttg
    22861 atatccagtt tagaatttgc tttgacaggt atttttactg ctatcaagga agaacgcaat
    22921 atgcgaaaac acgcagtgac ggctctagtg gtcatccttg caggttttgt ttttcaggtg
    22981 tcacgaatcg aatggctctt tctcctattg agtattttct tggtagtagc ctttgagatt
    23041 atcaactctg ctattgaaaa tgtggtggat ttggccagtc actatcactt ttccatgctg
    23101 gctaaaaatg ccaaggatat ggcggccggc gcggtattag tggtttctct tttcgcagcc
    23161 ttaacaggcg cattgatttt tctcccacga atctgggatt tattatttta aacagtaaga
    23221 ggaaattatg acttttaaat caggctttgt agccatttta ggacgtccca atgttgggaa
    23281 gtcaaccttt ttaaatcacg ttatggggca aaagattgcc atcatgagtg acaaggcgca
    23341 gacaacgcgc aataaaatca tgggaattta cacgactgat aaggagcaaa ttgtctttat
    23401 cgacacacca gggattcaca agcctaaaac agctctcgga gatttcatgg ttgagtctgc
    23461 ctacagtacc cttcgcgaag tggacactgt tcttttcatg gtgcctgctg atgaagcgcg
    23521 tggtaagggg gacgatatga ttatcgagcg tctcaaggct gccaaggttc ctgtgatttt
    23581 ggtggtgaat aaaatcgata aggtccatcc agaccagctc ttgtctcaga ttgatgactt
    23641 ccgtaatcaa atggacttta aggaaattgt tccaatctca gcccttcagg gaaataacgt
    23701 gtctcgtcta gtggatattt tgagtgaaaa tctggatgaa ggtttccaat atttcccgtc
    23761 tgatcaaatc acagaccatc cagaacgttt cttggtttca gaaatggttc gcgagaaagt
    23821 cttgcaccta actcgtgaag agattccgca ttctgtagca gtagttgttg actctatgaa
    23881 acgagacgaa gagacagaca aggttcacat ccgtgcaacc atcatggtcg agcgcgatag
    23941 ccaaaaaggg attatcatcg gtaaaggtgg cgctatgctt aagaaaatcg gtagcatggc
    24001 ccgtcgtgat atcgaactca tgctaggaga caaggtcttc ctagaaacct gggtcaaggt
    24061 caagaaaaac tggcgcgata aaaagctaga tttggctgac tttggctata atgaaagaga
    24121 atactaagta gaggtaggct catgcctgct tcttgttttt acagaaggag gacttatgcc
    24181 tgaattacct gaggttgaaa ccgtttgtcg tggcttagaa aaattgatta taggaaagaa
    24241 gatttcgagt atagaaattc gctaccccaa gatgattaag acggatttgg aagagtttca
    24301 aagggaattg cctagtcaga ttatcgagtc aatgggacgt cgtggaaaat atttgctttt
    24361 ttatctgaca gacaaggtct tgatttccca tttgcggatg gagggcaagt atttttacta
    24421 tccagaccaa ggacctgaac gcaagcatgc ccatgttttc tttcattttg aagatggtgg
    24481 cacgcttgtt tatgaggatg ttcgcaagtt tggaaccatg gaactcttgg tgcctgacct
    24541 tttagacgtc tactttattt ctaaaaaatt aggtcctgaa ccaagcgaac aagactttga
    24601 tttacaggtc tttcaatctg cccttgccaa gtccaaaaag cctatcaaat cccatctcct
    24661 agaccagacc ttggtagctg gacttggcaa tatctatgtg gatgaggttc tctggcgagc
    24721 tcaggttcat ccagctagac cttcccagac tttgacagca gaagaagcga ctgccattca
    24781 tgaccagacc attgctgttt tgggccaggc tgttgaaaaa ggtggctcca ccattcggac
    24841 ttataccaat gcctttgggg aagatggaag catgcaggac tttcatcagg tctatgataa
    24901 gactggtcaa gaatgtgtac gctgtggtac catcattgag aaaattcaac taggcggacg
    24961 tggaacccac ttttgtccaa actgtcaaag gagggactga tgggaaaaat catcggaatc
    25021 actgggggaa ttgcctctgg taagtcaact gtgacaaatt ttctaagaca gcaaggcttt
    25081 caagtagtgg atgccgacgc agtcgtccac caactacaga aacctggtgg tcgtctgttt
    25141 gaggctctag tacagcactt tgggcaagaa atcattcttg aaaacggaga actcaatcgc
    25201 cctctcctag ctagtctcat cttttcaaat cctgatgaac gagaatggtc taagcaaatt
    25261 caaggggaga ttatccgtga ggaactggct actttgagag aacagttggc tcagacagaa
    25321 gagattttct tcatggatat tcccctactt tttgagcagg actacagcga ttggtttgct
    25381 gagacttggt tggtctatgt ggaccgagat gcccaagtgg aacgcttaat gaaaagggac
    25441 cagttgtcca aagatgaagc tgagtctcgt ctggcagccc agtggccttt agaaaaaaag
    25501 aaagatttgg ccagccaggt tcttgataat aatggcaatc agaaccagct tcttaatcaa
    25561 gtgcatatcc ttcttgaggg aggtaggcaa gatgacagag attaactgga aggataatct
    25621 gcgcattgcc tggtttggta attttctgac aggagccagt atttctttgg ttgtaccttt
    25681 tatgcccatc ttcgtggaaa atctaggtgt agggagtcag caagtcgctt tttatgcagg
    25741 cttagcaatt tctgtctctg ctatttccgc ggcgctcttt tctcctattt ggggtattct
    25801 tgctgacaaa tacggccgaa aacccatgat gattcgggca ggtcttgcta tgactatcac
    25861 tatgggaggc ttggcctttg tcccaaatat ctattggtta atctttcttc gtttactaaa
    25921 cggtgtattt gcaggttttg ttcctaatgc aacggcactg atagccagtc aggttccaaa
    25981 ggagaaatca ggctctgcct taggtacttt gtctacaggc gtagttgcag gtactctaac
    26041 tggtcccttt attggtggct ttatcgcaga attatttggc attcgtacag ttttcttact
    26101 ggttggtagt tttctatttt tagctgctat tttgactatt tgctttatca aggaagattt
    26161 tcaaccagta gccaaggaaa aggctattcc aacaaaggaa ttatttacct cggttaaata
    26221 tccctatctt ttgctcaatc tctttttaac cagttttgtc atccaatttt cagctcaatc
    26281 gattggccct attttggctc tttatgtacg cgacttaggg cagacagaga atcttctttt
    26341 tgtctctggt ttgattgtgt ccagtatggg cttttccagc atgatgagtg caggagtcat
    26401 gggcaagcta ggtgacaagg tgggcaatca tcgtctcttg gttgtcgccc agttttattc
    26461 agtcatcatc tatctcctct gtgccaatgc ctctagcccc cttcaactag gactctatcg
    26521 tttcctcttt ggattgggaa ccggtgcctt gattcccggg gttaatgccc tactcagcaa
    26581 aatgactccc aaagccggca tttcgagggt ctttgccttc aatcaggtat tcttttatct
    26641 gggaggtgtt gttggtccca tggcaggttc tgcagtagca ggtcaatttg gctaccatgc
    26701 tgtcttttat gcgacaagcc tttgtgttgc ctttagttgt ctctttaacc tgattcaatt
    26761 tcgaacatta ttaaaagtaa aggaaatcta gtgcgagtaa aaatcaatct caaatgctcc
    26821 tcttgtggca gtatcaatta cctaaccagt aaaaattcaa aaacccatcc agacaagatt
    26881 gaggttttaa agtattgtcc caaggaaaga aaagtaaccc tacatcttga atctaagtag
    26941 attttatggt aaaataatag ggattttaag gagtttgata tgtataacct attattaacc
    27001 attttattag tattatctgt tgtgattgtg attgcaattt tcatgcaacc aaccaaaaac
    27061 caatccagca atgtatttga tgccagttca ggtgatttgt ttgaacgcag taaagctcgc
    27121 ggttttgaag ctgtaatgca gcgtttgaca gggattttag tctttttctg gctagccatt
    27181 gccttagcat tgacggtatt atcaagtaga taagaaaata atgggcagga ctaggtcttt
    27241 gcctcttttt atttttaaag gatgtttgag aaggttttac agtaaaagaa aattaaaaaa
    27301 tctagaaaga aaatatgaaa gatagaataa aagaatattt acaagacaag ggaaaggtga
    27361 ctgttaatga tttggctcag gctttgggaa aagacagttc caaggatttt cgtgagttga
    27421 ttaaaacctt gtccttaatg gaaagaaagc accaaattcg ttttgaagaa gatggtagtc
    27481 tgacattaga aattaagaaa aaacatgaga ttaccctcaa ggggattttt catgcccata
    27541 aaaatggctt tggctttgtt agtctggaag gcgaggagga cgaccttttt gtagggaaaa
    27601 atgatgtcaa ctatgctatt gatggtgata ccgtcgaggt agtgattaag aaagtcgctg
    27661 accgcaataa gggaacagca gcagaagcca aaattattga tatcctagaa cacagtttga
    27721 caacagttgt cgggcaaatc gttctggatc aggaaaaacc taagtatgct ggctatattc
    27781 gttcaaaaaa tcagaaaatc agtcaaccga tttatgttaa gaaaccagcc ctaaaattag
    27841 aaggaacaga agttctcaaa gtctttatcg ataaataccc aagcaagaaa catgatttct
    27901 ttgtcgcgag tgttctcgat gtagtgggac actcaacgga tgtcggaatt gatgttcttg
    27961 aggtcttgga atcaatggac attgtatccg agtttccaga agctgttgtt aaggaagcag
    28021 aaagtgtgcc tgatgctccg tctcaaaagg atatggaagg tcgtctggat ctaagagatg
    28081 aaattacctt taccattgac ggtgcggatg ccaaggactt ggacgatgca gtgcatatca
    28141 aggctctgaa aaatggcaat ctggagtttg gggttcacat cgcagatgtt tcttattatg
    28201 tgaccgaggg gtctgccctt gacaaggaag cccttaaccg tgcgacttct gtttacgtga
    28261 cagaccgagt ggtgccaatg cttccagaac gactatcaaa tggcatctgc tctctcaatc
    28321 cccaagttga ccgcctgacc cagtctgcta ttatggagat tgataaacat ggtcgtgtgg
    28381 tcaactatac cattacacaa acagttatca agaccagttt tcgtatgacc tatagcgatg
    28441 tcaatgatat cctagctggc gatgaagaaa agagaaaaga atatcataaa attgtatcaa
    28501 gtatcgaact catggccaag cttcatgaaa ctttagaaaa catgcgtgtg aaacgtggag
    28561 ctctcaattt tgataccaat gaagcgaaga ttttagtgga taaacaaggt aagcctgttg
    28621 atatcgttct tcggcagcgt ggtattgccg agcggatgat tgagtctttt atgttgatgg
    28681 ctaatgaaac agttgccgaa catttcagca agttggattt gccttttatc tatcgaattc
    28741 acgaggagcc taaggctgaa aaggttcaga agtttattga ttatgcttcg agttttggct
    28801 tgcgcattta tggaactgcc agtgagatta gtcaggaggc acttcaagac atcatgcgtg
    28861 ctgttgaggg agaaccttat gcagatgtat tgtccatgat gcttcttcgc tctatgcagc
    28921 aggctcgtta ttcggagcac aatcacggcc actatggact agctgctgac tattatactc
    28981 actttaccag tccaattcgt cgttatccag accttcttgt tcaccgtatg attcgggatt
    29041 acggccgttc taaggaaata gcagagcatt ttgaacaagt gattccagag attgcgaccc
    29101 agtcttccaa ccgtgaacgt cgtgccatag aagctgagcg tgaagtcgaa gccatgaaaa
    29161 aggctgagta tatggaagaa tacgtgggtg aagagtatga tgcagttgta tcaagtattg
    29221 tcaaattcgg tctctttgtc gaattgccaa acacagttga aggcttgatt cacatcacta
    29281 atctgcctga attttatcat ttcaatgagc gtgatttgac tcttcgtgga gaaaaatcag
    29341 gtatcacttt ccgagtgggt cagcagatcc gtatccgtgt tgaaagagcg gataaaatga
    29401 ctggagagat tgatttttca ttcgtaccta gtgagtttga tgtgattgaa aaaggcttga
    29461 aacagtctag tcgtagtggc agagggcgtg attcaaatcg tcgttcggat aagaaggaag
    29521 acaagagaaa atcaggacgc tcaaatgata agcgtaagca ttcacaaaaa gacaagaaga
    29581 aaaaaggaaa gaaacctttt tacaaggaag tagctaagaa aggagccaag catggcaaag
    29641 ggcgagggaa aggtcgtcgc acaaaataaa aaggcacgcc acgactatac aatcgtagat
    29701 acgctagagg cagggatggt cctgactgga actgaaatca agagtgtacg agctgctcga
    29761 attaatctca aggatggctt tgctcaagtg aaaaatggag aagtttggct gagcaatgtt
    29821 catatcgcgc cttacgaaga gggcaatatc tggaaccagg aaccagaacg tcgtcgtaaa
    29881 ctcctgctcc ataaaaagca aattcaaaaa ttggaacaag agatcaaagg gacaggaatg
    29941 accttagttc cccttaaggt ctatataaaa gatggctacg ctaagcttct tttaggactt
    30001 gccaaaggga agcatgacta tgacaaacgg gagtctatca aacgtcgtga gcaaaatcga
    30061 gatatcgcgc gtgtgatgaa agctgttaat cagcgataaa aagaggaatt gaaaatggaa
    30121 aaattagttg cctataaacg catgcctttg tggaataaac aaacaatgcc tgaagctgtt
    30181 cagcaaaagc acaatacaaa agttgggact tgggggaaaa ttactgtctt gaagggagct
    30241 ctcaagttta ttgaattgac agaagaaggg gaagttctag ctgaacacct ctttgaagca
    30301 ggggcagaca atccaatggc ccaacctcaa gcctggcacc gagtggaagc tgccacagat
    30361 gatgtggaat ggtacttgga attttattgt aaacctgagg attattttgc taaaaaatac
    30421 aataccaatc ctgttcattc agaggtccta gaggccatgc agacagtgaa acaagggaaa
    30481 gctttggatt tgggttgtgg tcaggggcgt aattctcttt ttctagccca gcaagatttt
    30541 gatgtgacgg ctgtagatca aaatggacta gctcttgaaa tcttgcaaag cattgtggag
    30601 caggaagatt tggacatgcc tgttggcctt tacgatatca attcagctag cattgaacaa
    30661 gaatatgatt ttatcgtttc aacagttgtt ctcatgtttc tacaagcgga ccgcatttca
    30721 gctattattc aaaatatgca ggagaaaacc agtgttggtg gttacaacct tatcgtttgt
    30781 gccatggaca cggaggatta tccttgctcg gttaacttcc cattcacctt taaagaagga
    30841 gaactggcag actattacaa ggattgggaa ttggttaagt acaatgaaaa tccaggccat
    30901 ttgcaccgtc gcgatgagaa tggcaatcgt attcaactac gctttgcgac cttactagct
    30961 aagaaaatca agtaaacaca catgaagatt aggaattttc ctgatctttt ttctttttta
    31021 cgaatgatat agaaaaggag ggaattcatg tttgttgcga gagatgctag gggagaattg
    31081 gtaaatgtgt tagaggataa acttgagaag caagcataca cctgcccagc ttgtggaggc
    31141 cagctccatt tgcgtcaagg accaagtgta cggacgcatt ttgcccataa atccttaaaa
    31201 gactgtgatt ttttctttga aaatgaaagt ccagaacacc tggccaataa ggaatccctc
    31261 tatcactggt tgaaaaaaga gacaaaggtt caattagagt acccgctttc agaacttaaa
    31321 cagattgcgg atgtatttgt aaatggcaat ctagctctag aagttcagtg tagtcccttg
    31381 cctcagaaag tccttaaaga gcgaagtgag ggctatcgta gtcagggtta ccaagtactg
    31441 tggttgctgg gtcaaaaact gtggctcaag gagcgtttga ctcgtctaca gcaaggtttt
    31501 ctttatttca gtcaaaacat gggcttttat gtttgggaat tagacaagga aaaacaagtt
    31561 ttaagactca aatacctgat ttaccaggat ctccgcggta aactccatta tcaaatcaag
    31621 gaattttcct atggtcaagg tagtttattg gaaatattgc gtcttcccta taagagacaa
    31681 aaaatatctc attttacagt ttctgaggac aaggacatct gtcgctatat ccggcaacaa
    31741 ctttattatc aaaatctctt ttggatgaaa gaacaagcag aagcctatca aaagggagaa
    31801 aatatcctga cttatggact gaaagaatgg tatccacaaa ttcgaccaat agtgggcaaa
    31861 tttttccaga ttgaacaaga cttgactagc tattatcagc acttttatac ctattaccaa
    31921 aaaaatcctc aaaatgattg gcaaaagctt tatccaccag ccttttatca gcaatatttc
    31981 ttgaaaaata tggtagaata gaaaggatgg aggaatctaa tggtattaca aagaaatgaa
    32041 ataaatgaaa aagatacatg ggatctatca acgatctacc caactgacca ggcttgggaa
    32101 gaagccttaa aagatttaac agaacaattg gagacagtag cccagtatga aggccatctc
    32161 ttggatagtg cggataacct actagaaatc actgaatttt ctcttgaaat ggaacgccag
    32221 atagagaagc tttacgctta tgctcatatg aagaatgacc aggatacacg tgaagctaag
    32281 tatcaagagt actatgccaa ggccatgaca ctctacagcc agttagacca agccttttca
    32341 ttctatgagc ctgaatttat ggagattagc gaaaagcagt atgctgactt tttagaagct
    32401 caaccaaagc tgcaggttta tcaacactat tttgacaagc ttttgcaagg caaggatcac
    32461 gttctttcac aacgtgaaga agaattattg gctggagctg gagaaatctt tggttcagca
    32521 agtgaaacct tcgctatctt ggacaatgcg gatattgtgt tcccttatgt cctagacgat
    32581 gatggtaaag aagttcagct atctcatggg acttacacac gtttgatgga gtctaaaaaa
    32641 cgtgaggttc gccgtggtgc ctatcaagct ctttatgcga cttacgaaca attccaacac
    32701 acctatgcca aaaccttgca aaccaatgtt aaggtgcaaa attaccgtgc taaagttcgt
    32761 aactacaaga gtgctcgtca tgcagccctc gcagcgaatt ttgttccaga aagtgtttat
    32821 gacaatttgg tagcagcagt tcgcaagcat ttgccactct tacatcgcta tcttgagctt
    32881 cgttcaaaaa tcttggggat ttcagatctc aagatgtacg atgtctacac accgctttca
    32941 tctgttgaat acagttttac ctaccaagaa gccttgaaaa aagcagaaga tgctttggca
    33001 gtcttgggtg aggattactt gagccgtgtt aaacgtgcct tcagcgagcg ttggattgat
    33061 gtttacgaaa atcaaggcaa gcgttcaggt gcctactctg gtggttctta tgataccaat
    33121 gcctttatgc ttctcaactg gcaagacaat ctggacaatc tctttactct tgttcatgaa
    33181 acaggtcaca gtatgcattc aagctatact cgtgaaactc agccttatgt ttacggggat
    33241 tactctatct ttttggctga gattgcctca actaccaatg aaaatatctt gacggagaaa
    33301 ttattggaag aagtggaaga cgacgcaaca cgctttgcta ttctcaataa cttcctagat
    33361 ggtttccgtg gaacagtttt ccgccaaact caatttgctg agtttgaaca cgccattcac
    33421 caagcagatc aaaatgggga ggtcttgaca agcgatttcc taaataaact ctacgcagac
    33481 ttgaaccaag agtattatgg tttgagtaag gaagacaatc ctgaaatcca atacgagtgg
    33541 gctcgcattc cacacttcta ctataactac tatgtatatc aatattcaac tggctttgcg
    33601 gccgcctcag ccttggctga aaaaattgtc catggtagtc aagaagaccg tgaccgctat
    33661 atcgactacc tcaaggcagg taagtcggac tatccactta atgtcatgag aaaagctggt
    33721 gttgatatgg agaaggaaga ctacctcaac gatgcctttg cagtctttga acgccgttta
    33781 aatgagtttg aagcccttgt tgaaaaatta ggattggcat aaaatggttg aatcgtatag
    33841 taagaatgct aaccataaca tgcgtcgtcc tgtcgtcaaa gaagaaattg tagacttgat
    33901 gcgtcagcgt caaaagcagg tcacaggttt cttgaaagaa ttggaagact ttgcccgcaa
    33961 ggaaaatatt cctattattc cccatgaaac ggttgcttat ttccgttttc ttatggaaac
    34021 catgcagcct aaaaatattc tggaaattgg gacggctatc ggtttttcag ctctcttgat
    34081 ggctgaacat gcgccaaatg ctaagattac aactattgat cgtaatccag aaatgattgg
    34141 ttttgccaag gaaaattttg cccagtttga cagtcgcaag caaatcactc tcctagaggg
    34201 agatgcggtg gatgtcttat ctacactgac agagtcttat gatttcgtct ttatggattc
    34261 tgccaagtct aaatacatcg tctttctgcc agaaatcctc aaacatttgg aagttggtgg
    34321 tgtggttgtc ttggatgata tttttcaagg tggtgatgtt gccaaggata ttatggaagt
    34381 ccgtcgtggt cagcgaacca tttatcgagg ccttcaaaaa ttatttgatg caaccttaga
    34441 caatccagaa ctcaccgcaa cattagtgcc tttaggagat ggtattctca tgcttcgtaa
    34501 aaatgtagca gatgttcaac tgtctgaaag cgaatgattt tcagaaaaat ttaagaaaaa
    34561 atagtaaaat agatagagta acacttatct caaaggagta gacatgaaga aaaaattatt
    34621 ggcaggtgcc atcacactat tatcagtagc aactttagca gcttgttcga aagggtcaga
    34681 aggtgcagac cttatcagca tgaaagggga tgtcattaca gaacatcaat tttatgagca
    34741 agtgaaaagc aacccttcag cccaacaagt cttgttaaat atgaccatcc aaaaagtttt
    34801 tgaaaaacaa tatggctcag agcttgatga taaagaggtt gatgatacta ttgccgaaga
    34861 aaaaaaacaa tatggcgaaa actaccaacg tgtcttgtca caagcaggta tgactcttga
    34921 aacacgtaaa gctcaaattc gtacaagtaa attagttgag ttggcagtta agaaggtagc
    34981 agaagctgaa ttgacagatg aagcctataa gaaagccttt gatgagtaca ctccagatgt
    35041 aacggctcaa atcatccgtc ttaataatga agataaggcc aaagaagttc tcgaaaaagc
    35101 caaggcagaa ggtgctgatt ttgctcaatt agccaaagat aattcaactg atgaaaaaac
    35161 aaaagaaaat ggtggagaaa ttacctttga ttctgcttca acagaagtac ctgagcaagt
    35221 caaaaaagcc gctttcgctt tagatgtgga tggtgtttct gatgtgatta cagcaactgg
    35281 cacacaagcc tacagtagcc aatattacat tgtaaaactc actaagaaaa cagaaaaatc
    35341 atctaatatt gatgactaca aagaaaaatt aaaaactgtt atcttgactc aaaaacaaaa
    35401 tgattcaaca tttgttcaaa gcattatcgg aaaagaattg caagcagcca atatcaaggt
    35461 taaggaccaa gccttccaaa atatctttac ccaatatatc ggtggtggag attcaagctc
    35521 aagcagtagt acatcaaacg aatagtccaa atcaatgagt cagggaaaaa actcgacttc
    35581 aggaaaaaat gaagcaaaca ttcccacaat aaaacgcata gtacaaggtt tgtactgccc
    35641 cccaaaaagt tagacaatta atttatccga aggatttagt tctgtattgc acagagctaa
    35701 gtccttttag ttttatctta attctcttat tgttgtaata atcaatatag tctataatgg
    35761 ct
//
LOCUS       DAVKNL010000018        34405 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVKNL010000018 DAVKNL010000000
VERSION     DAVKNL010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34405
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.018"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            556..1008
                     /locus_tag="VW411_000866"
     CDS             556..1008
                     /locus_tag="VW411_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU9793623.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            1027..2364
                     /locus_tag="VW411_000867"
     CDS             1027..2364
                     /locus_tag="VW411_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEU9793624.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2443..3327
                     /locus_tag="VW411_000868"
     CDS             2443..3327
                     /locus_tag="VW411_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9793625.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3327..4160
                     /locus_tag="VW411_000869"
     CDS             3327..4160
                     /locus_tag="VW411_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9793626.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAVFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4178..6271
                     /gene="nanB"
                     /locus_tag="VW411_000870"
     CDS             4178..6271
                     /gene="nanB"
                     /locus_tag="VW411_000870"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEU9793627.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISSLLLDKLSGESQTVVMKFKADKPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSTIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     NETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLRERHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSKVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6283..7386
                     /locus_tag="VW411_000871"
     CDS             6283..7386
                     /locus_tag="VW411_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU9793628.1"
                     /translation="MVKYGVVGTGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7560..8258
                     /locus_tag="VW411_000872"
     CDS             7560..8258
                     /locus_tag="VW411_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEU9793629.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8277..9794
                     /locus_tag="VW411_000873"
     CDS             8277..9794
                     /locus_tag="VW411_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9793630.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADLAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPFGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            9919..11247
                     /locus_tag="VW411_000874"
     CDS             9919..11247
                     /locus_tag="VW411_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9793631.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11499..12365
                     /locus_tag="VW411_000875"
     CDS             11499..12365
                     /locus_tag="VW411_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9793632.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            12381..13220
                     /locus_tag="VW411_000876"
     CDS             12381..13220
                     /locus_tag="VW411_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9793633.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13492..13944
                     /locus_tag="VW411_000877"
     CDS             13492..13944
                     /locus_tag="VW411_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU9793634.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYDIDGD
                     KVFLVVQENVLNQAENDQFEYHKNYADLHLLVEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            14041..14385
                     /locus_tag="VW411_000878"
     CDS             14041..14385
                     /locus_tag="VW411_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793635.1"
                     /translation="MVSSKYAARASFLDGQGITVDEMAWIIRGIVNALIGRYIKLGTY
                     AAKYGISMARSILSRVAATAAARVGLLTKISGWILRVAVNVADVYGNFANNIAAAWDV
                     YDKIPNNGRINF"
     gene            14662..15306
                     /locus_tag="VW411_000879"
     CDS             14662..15306
                     /locus_tag="VW411_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU9793636.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            15475..16392
                     /locus_tag="VW411_000880"
     CDS             15475..16392
                     /locus_tag="VW411_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU9793637.1"
                     /translation="MSDLKKYEGVIPAFYACYDDQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGARAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            16410..17294
                     /locus_tag="VW411_000881"
     CDS             16410..17294
                     /locus_tag="VW411_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9793638.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17310..18161)
                     /locus_tag="VW411_000882"
     CDS             complement(17310..18161)
                     /locus_tag="VW411_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEU9793639.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18361..20418
                     /gene="pbp2b"
                     /locus_tag="VW411_000883"
     CDS             18361..20418
                     /gene="pbp2b"
                     /locus_tag="VW411_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEU9793640.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKIVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSTSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            20429..21025
                     /gene="recR"
                     /locus_tag="VW411_000884"
     CDS             20429..21025
                     /gene="recR"
                     /locus_tag="VW411_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEU9793641.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21201..22244
                     /locus_tag="VW411_000885"
     CDS             21201..22244
                     /locus_tag="VW411_000885"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEU9793642.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVDYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNNITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPKLIERLVDL
                     AKESFDKREAHLI"
     gene            22328..23701
                     /locus_tag="VW411_000886"
     CDS             22328..23701
                     /locus_tag="VW411_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEU9793643.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQDLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSVQLHNQMILSLSPDVLDTVIFYGENIAE
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAHDQILLKGSNSMNLAKLVE
                     SLENEDK"
     gene            23688..24299
                     /locus_tag="VW411_000887"
     CDS             23688..24299
                     /locus_tag="VW411_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9793644.1"
                     /translation="MKTSDFVKYLQRMIAITDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQASDLDSEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYTKFVFGCKLLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIELLWQVYQGHRGQYLD"
     gene            24390..25094
                     /locus_tag="VW411_000888"
     CDS             24390..25094
                     /locus_tag="VW411_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEU9793645.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            25313..26686
                     /gene="ftsA"
                     /locus_tag="VW411_000889"
     CDS             25313..26686
                     /gene="ftsA"
                     /locus_tag="VW411_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEU9793646.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26703..27962
                     /gene="ftsZ"
                     /locus_tag="VW411_000890"
     CDS             26703..27962
                     /gene="ftsZ"
                     /locus_tag="VW411_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU9793647.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            27967..28638
                     /locus_tag="VW411_000891"
     CDS             27967..28638
                     /locus_tag="VW411_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU9793648.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            28648..29187
                     /locus_tag="VW411_000892"
     CDS             28648..29187
                     /locus_tag="VW411_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEU9793649.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTSVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            29187..29450
                     /locus_tag="VW411_000893"
     CDS             29187..29450
                     /locus_tag="VW411_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEU9793650.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            29447..30232
                     /locus_tag="VW411_000894"
     CDS             29447..30232
                     /locus_tag="VW411_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEU9793651.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            30241..31029
                     /gene="divIVA"
                     /locus_tag="VW411_000895"
     CDS             30241..31029
                     /gene="divIVA"
                     /locus_tag="VW411_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEU9793652.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMAELQARIEV
                     ADKELSEFEAQIKQEVEAPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            31377..34169
                     /locus_tag="VW411_000896"
     CDS             31377..34169
                     /locus_tag="VW411_000896"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEU9793653.1"
                     /translation="MKLKDTLNLGKTEFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGEARKFVVAAELLTSLSEK
                     FGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMVAETIEHVAQLFEEYGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPELTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDTVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFETEDFVQLSELPEVQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIAEGPAPEAALSFEDVAFTVERATGEVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAEGFEEK"
BASE COUNT        10580 a         6065 c         7484 g        10276 t
ORIGIN      
        1 aaactaattt agagaatgaa atatagagta tttctctctt aaaagttttt ggtgaaacga
       61 gatgtagaaa ggagatttag ccaaagagtc tattagtgct agaataatag attagaatta
      121 ttttagaaaa acgaagtgag cagcttataa attcaagtcc ccaaatagat tcatactagt
      181 atcttttgca aaaaataaag ggcgacttcc ttcatgaata tcaatttcat ctataaggaa
      241 ggtagctaat tgaactaact tatttattct gtttgtcgct agaaaaatca gacctccttg
      301 tgaagattga ggagatactt aatgaaaatc aaagaagaaa ctagcaagct agtagcagat
      361 tgcccaaaac accgctttga ggttgtagat aagactgacc tatataatcc aaggtgaagc
      421 gactgtggtt tgaagagatt ttcaaagagt ataggctaga gagtagtgtt tttatgtcct
      481 tctagtagaa aatgctagac agaagaatgg ggaacttgga taggaaaaat agattgagaa
      541 aggaggttag aagagatgat tattacaaaa attagccgtt taggaactta tgtgggagta
      601 aatccacatt ttgcaacatt aatagatttt ctagaaaaaa caggactaga aaatttaaca
      661 gaaggttcga ttgctatcga tggtaatcga ttgtttggga attgctttac ttatctagca
      721 gatggtcaag caggggcttt ctttgaaacc caccaaaaat atttggatat tcatttagtt
      781 ttggaaaacg aagaagccat ggctgttaca tcgccggaaa atgtaagcgt tacccaagaa
      841 tatgatgaag agaaagatat tgaattatac acagggaaag tggaacagtt ggttcatttg
      901 agagctggcg aatgcctcat cacttttcca gaagatttac atcaacccaa ggttcgtata
      961 aatgatgaac ctgtgaaaaa agttgtcttt aaagttgcga tttcttaatg tagaaagaga
     1021 agaacgatga aaaaaatgag aaagttttta tgtctagctg gaattgcgct agcggctgtt
     1081 gccttggtag cttgttcagg aaaaaaagaa gctacaacta gtactgaacc accaacagaa
     1141 ttatctggtg agattacaat gtggcactcc tttactcaag gaccccgttt agaaagtatt
     1201 caaaaatcag cagatgcttt catgcaaaag catccaaaaa cgaaaatcaa gattgaaaca
     1261 ttttcttgga atgacttcta tactaaatgg actacaggtt tagcaaatgg aaatgtgcca
     1321 gatatcagta cagctcttcc taaccaagta atggaaatgg tcaactcaga tgctttggtt
     1381 ccgctaaatg attctatcaa gcgtattgga caagataaat ttaacgaaac tgccttaaat
     1441 gaagcaaaaa tcggagatga ttactactct gttcctcttt attcacatgc acaagtcatg
     1501 tgggttagaa cagatttgtt aaaagaacat aatattgagg ttcctaaaac ttgggatcaa
     1561 ctctatgaag cttctaaaaa attgaaagaa gctggagttt atggcttgtc tgttccgttt
     1621 ggaacaaatg acttaatggc aacacgtttc ttgaacttct acgtacgtag tggtggagga
     1681 agcctcttaa caaaagatct taaagcagac ttgacaagcc aacttgctca agatggtatt
     1741 aaatactggg ttaaattgta taaagaaatc tcacctcaag attctttgaa ctttaatgtc
     1801 cttcaacaag ctaccttgtt ctatcaagga aaaacagcat ttgactttaa ctctggcttc
     1861 catatcggag gaattaatgc caacagtcct caattgattg attcgattga tgcttatcct
     1921 attccaaaaa tcaaagagtc tgataaagac caaggaattg aaacctcaaa cattccaatg
     1981 gttgtttgga aaaattcaaa acatccagaa gttgctaaag cattcttaga agcactttat
     2041 aatgaagaag actacgttaa attccttgat tcaactccag taggtatgtt gccaactatt
     2101 aaggggatta gcgattctgc agcctataaa gaaaatgaaa ctcgtaagaa atttaaacat
     2161 gctgaagaag taattactga agctgttaaa aaaggtactg ctattggtta tgaaaatggg
     2221 ccaagtgtac aagctggtat gttgactaac caacacatta ttgaacaaat gttccaagat
     2281 atcattacaa atggaacaga tcctatgaaa gcagcaaaag aagcagaaaa acaattaaat
     2341 gatttatttg aggctgttca gtagatgtaa aagactagaa aataggtggg atagtgagct
     2401 gaaaagctct agcccaatct tgtaaaagaa gggagaagga gaatggttaa agaacgtaat
     2461 ttaactcgct ggatatttgt tttgccagct atgattatcg taggattact ctttgtttat
     2521 ccgtttttct cgagtatttt ttatagcttt accaataagc atttgattat gcctaattat
     2581 aaatttgttg gtttggctaa ctataaagct gtgctatcag atcccaactt ctttaatgcg
     2641 ttctttaatt caattaagtg gaccgttttc tcattagttg gtcaagtttt agtagggttt
     2701 gtattggctt tagctcttca cagagtacgc cacttcaaga aattatatag gacattattg
     2761 attgttcctt gggcatttcc taccatcgtt attgccttct cttggcagtg gattctaaac
     2821 ggggtttatg gctacttacc taatctaatc gtaaaattag gtttaatgga acatacacct
     2881 gcatttttga cagatagtac atgggcattc ctatgtttgg tgtttatcaa catttggttt
     2941 ggagcaccaa tgattatggt taatgtgctt tcagctttgc aaacagtacc agaagaacaa
     3001 tttgaggctg ctaagataga tggtgcttca agttggcagg tgttcaagtt tatcgtcttt
     3061 ccacatatta aagtggttgt aggacttcta gttgttttga gaactgtatg gatctttaat
     3121 aactttgaca ttatctacct cattactggt ggtggaccag ccaatgctac aacgacgctt
     3181 ccaatttttg cttacaacct gggctgggga actaaattgt tgggtcgtgc ttcagcagtt
     3241 acagtactgc tctttatctt cttggtggcg atttgcttta tctactttgc tatcatcagt
     3301 aagtgggaaa aggagggtag aaaataatga agaagaaatc cagtatttat ttagatattc
     3361 tctcacatgt acttttagtt ggtgcgacca tcgttgcagt tttcccattg gtatggatta
     3421 tcatatcttc tgtcaaaggg aaaggggaat taactcagta tccaacacga ttttggcctg
     3481 aacagtttac attagattat ttcactcatg ttatcaacga tttgcacttc attgataaca
     3541 ttcgaaacag tttaatcatt gccttggcta caacccttat tgcgattatt atttctgcta
     3601 tggcagccta tggtattgtt cgattctttc ctaaattggg agcaatcatg tcgagactac
     3661 tcgtcattac ctacattttc ccaccaattt tgttagcaat tccctattca attgccattg
     3721 ctaaagttgg gttaacaaat agtttatttg gcttgatgat ggtttatcta tcttttagtg
     3781 ttccatatgc agtttggctc ttagttggat ttttccaaac agttccaatt ggaattgaag
     3841 aagcggctag aattgatggt gcaaataaat ttgttacgtt ttataaagtt gtgctaccga
     3901 ttgtagcacc aggtattgta gcaacagcta tttatacatt tatcaatgct tggaatgaat
     3961 tcctgtatgc cttgattttg attaacaata caggaaagat gacagtagca gtagcccttc
     4021 gttcacttaa tggttcagaa atactagact ggggagatat gatggcagcg tctgttattg
     4081 tagttcttcc atcaattatt ttcttctcta tcatccaaaa taagattgca agtggattat
     4141 cagaaggatc tgtgaagtag acgaaagaag gaaaaaaatg aataaaagag gtctttattc
     4201 aaaactagga atttccgttg taggcattag tcttttaatg ggagtcccca ctttgattca
     4261 tgcgaatgaa ttaaactatg gtcaactgtc catatctcct atttttcaag gaggttcata
     4321 tcaactgaac aataagagta tagatatcag ctctttgtta ttagataaat tgtctggaga
     4381 gagtcagaca gtagtaatga aatttaaagc agataaacca aactctcttc aagctttgtt
     4441 tggcctatct aatagtaaag caggctttaa aaataattac ttttcaattt tcatgagaga
     4501 ttctggtgag ataggtgtag aaataagaga cgcccaaaag ggaataaatt atttattttc
     4561 cagaccagct tcattatggg gaaaacataa aggacaggca gttgaaaata cactagtatt
     4621 tgtatctgat tctaaagata aaacatacac aatgtatgtt aatggaatag aagtgttctc
     4681 tgaaacagtt gatacatttt tgccaatttc aaatataaat ggtatagata aggcaacact
     4741 aggagctgtt aatcgtgaag gtaaggaaca ttacctcgca aaaggaagta ttgatgaaat
     4801 cagtctattt aacaaagcaa ttagtgatca ggaagtttca actattccct tgtcaaatcc
     4861 atttcagtta attttccaat caggagattc tactcaagct aactatttta gaataccgac
     4921 actatataca ttaagtagtg gaagagttct atcaagtatt gatgcacgtt atggtgggac
     4981 tcatgattct aaaagtaaga ttaatattgc cacttcttat agtgatgata atgggaaaac
     5041 gtggagtgag ccaatttttg ctatgaagtt taatgactat gaggagcagt tagtttactg
     5101 gccacgagat aataaattaa agaatagtca aattagtgga agtgcttcat tcatagattc
     5161 atccattgtt gaagataaaa aatctgggaa aacgatatta ctagctgatg ttatgcctgc
     5221 gggtattgga aataataatg caaataaagc cgactcaggt tttaaagaaa taaatggtca
     5281 ttattattta aaactaaaga agaatggaga taacgatttc cgttatacag ttagagaaaa
     5341 tggtgtcgtt tataatgaaa caactaataa acctacaaat tatactataa atgataagta
     5401 tgaagttttg gagggaggaa agtctttaac agtcgaacaa tattcggttg attttgatag
     5461 tggctcttta agagaaaggc ataatggaaa acaggttcct atgaatgttt tctacaaaga
     5521 ttcgttattt aaagtgactc ctactaatta tatagcaatg acaactagtc agaatagagg
     5581 agagagttgg gaacaattta agttgttgcc tccgttctta ggagaaaaac ataatggaac
     5641 ttacttatgt cccggacaag gtttagcatt aaaatcaagt aacagattga tttttgcaac
     5701 atatactagt ggagaactaa cctatctcat ttctgatgat agtggtcaaa catggaagaa
     5761 atcctcagct tcaattccgt ttaaaaatgc aacagcagaa gcacaaatgg ttgaactgag
     5821 agatggtgtg attagaacat tctttagaac cactacaggt aagatagctt atatgactag
     5881 tagagattct ggagaaacat ggtcgaaagt ttcgtatatt gatggaatcc aacaaacttc
     5941 atatggcaca caagtatctg caattaaata ctctcaatta attgatggaa aagaagcagt
     6001 cattttgagt acaccaaatt ctagaagtgg ccgcaaggga ggccaattag ttgtcggttt
     6061 agtcaataaa gaagatgata gtattgattg gaaataccac tatgatattg atttgccttc
     6121 gtatggttat gcctattctg cgattacaga attgccaaat catcacatag gtgtactgtt
     6181 tgaaaaatat gattcgtggt cgagaaatga attgcattta agcaatgtag ttcagtatat
     6241 agatttggaa attaatgatt taacaaaata aaggagaaaa acatggttaa atacggtgtt
     6301 gttggaacag ggtattttgg agctgaattg gctcgctaca tgcaaaagaa tgatggagca
     6361 gagattactc ttctctatga tccagataat gcagaggcga ttgcagaaga attgggagca
     6421 aaagtagcaa gttccttaga tgagttggtt tctagcgatg aagtagattg tgttatcgtc
     6481 gcaactccaa ataatcttca taaggaaccg gttattaagg ctgcacagca tggtaaaaat
     6541 gttttctgtg aaaaaccaat tgcgctttct tatcaagatt gtcgcgagat ggtagatgcg
     6601 tgtaaagaaa acaatgtaac ctttatggca ggacatatta tgaatttctt taatggtgtt
     6661 catcatgcaa aagaactcat taatcaagga gttatcggag acgttctata ttgtcataca
     6721 gctcgtaatg gttgggaaga acaacaaccg tcagtatcat ggaaaaaaat tcgtgaaaaa
     6781 tcaggtggtc acttgtatca ccacatccat gaattggatt gcgttcaatt ccttatgggg
     6841 ggcatgcctg aaactgtaac catgacaggt ggaaatgtgg cccatgaagg tgaacatttc
     6901 ggtgatgaag atgatatgat ttttgtcaat atggaatttt ctaataagcg ttttgccttg
     6961 ttagaatggg gttcagctta tcgttggggt gaacattatg tcttaatcca aggaagcaaa
     7021 ggtgccatcc gcttagactt attcaactgt aaaggaactc ttaagctaga tgggcaagaa
     7081 agctatttct tgattcacga atcgcaagaa gaagatgatg atcggactcg tatctatcat
     7141 agtacagaga tggatggagc aattgcttat ggtaaaccag gtaaacgtac tccattatgg
     7201 ctatcatctg tcattgataa agaaatgcgc tatctgcatg agattatgga aggagctcca
     7261 gtatcagaag aatttgcaaa acttttgaca ggtgaagctg ccctagaagc aattgctact
     7321 gcagatgctt gtacccagtc tatgtttgaa gatcgcaaag taaaattgtc agaaattgta
     7381 aaataaattt tggtattctc ctatttatag gtcgacttgc tcctctgaaa gtacttttag
     7441 aggagctgtt tgactttgct agtttttgaa actgaaatct attatactac aaactattga
     7501 aagcgtttta attttaaggt ataataatct catagaaata aagaaaagga ggaaagagga
     7561 tgccacagat tagcaaagaa gccttgattg agcaaatcaa agatggaatc atcgtttctt
     7621 gtcaggctct tcctcatgaa ccgctttata cagaagcggg aggggtgatt cccttgctgg
     7681 tcaaagcggc tgagcaaggt ggagcagtcg gtatccgagc aaacagtgtt cgcgatatca
     7741 aggaaattaa ggaagtcact aaacttccaa tcattgggat tatcaaacgt gattatccac
     7801 ctcaggaacc cttcatcacg gctactatga aagaagttga tgaattggca gaactggaca
     7861 tcgaggtgat tgctctggat tgtaccaagc gtgaacgcta cgatggtttg gaaattcaag
     7921 agttcattcg tcaggttaag gagaaatatc ctaatcagct tttgatggct gatactagta
     7981 tcttcgaaga agggctagca gctgtagaag caggaattga ctttgtcgga acaaccttat
     8041 caggctacac atcctacagt ccaaaagtag acggtccaga ttttgaattg attaagaaac
     8101 tctgtgatgc tggtgtagat gtcattgcag aaggaaaaat tcatacacca gaacaagcca
     8161 aacaaatcct tgaatatgga gtgcgaggca tcgttgttgg tggcgccatt actagaccaa
     8221 aagagattac agaacgcttc gttgctagtc ttaaataaga tgtgaggggg agttttatgt
     8281 ttaaagtttt acaaaaagtt ggaaaagctt ttatgttacc tatagctata cttcctgcag
     8341 caggtctact tttggggatt ggtggtgcac tttcaaaccc aaccacgata gcaacttatc
     8401 caatactaga caatagtatt tttcaatcaa tattccaagt aatgagctct gcaggagagg
     8461 ttgtattcag taatttgtca ctacttctct gtgtgggatt atgtattggc ttagcgaaac
     8521 gagataaagg aaccgctgcg ttagcaggag taactggtta cttagttatg actgcaacga
     8581 tcaaagcttt ggtaaaactt tttatggcag aaggatctgc aattgatact ggagttattg
     8641 gagcattagt tgtcggaata gttgccgtat atttgcacaa ccgatataac aatattcaat
     8701 taccttccgc tttaggattc tttggaggtt cacgcttcgt tcctattgtt acatcgttct
     8761 cttctatctt gattggcttt gtcttctttg ttatttggcc acctttccaa caacttcttg
     8821 tttctacagg tggatatatt tctcaggcgg gtccaattgg aacttttcta tatggatttt
     8881 taatgagact ttctggagca gtaggcttac atcatataat ttaccctatg ttttggtata
     8941 ctgaacttgg tggtgttgaa actgttgcag gacaaacagt ggttggagct caaaaaatat
     9001 tttttgctca attagccgat ttggcccatt ctggattatt tacagaagga acaaggtttt
     9061 ttgcaggtcg tttctcaaca atgatgttcg gtttaccggc tgcctgttta gcgatgtacc
     9121 atagtgttcc taaaaatcgt cgtaaaaaat acgcgggttt gttttttgga gttgctttaa
     9181 catcttttat taccggtatt acagaaccaa ttgaatttat gtttctattc gtcagtccgg
     9241 ttctatatgt tgttcacgca ttccttgatg gtgttagctt ctttattgca gacgtcttaa
     9301 atatttcaat aggaaacaca ttttcaggag gtgtaatcga tttcacttta tttggaattt
     9361 tgcaggggaa cgctaagacg aattgggttc ttcagattcc atttggactt atttggagtg
     9421 ttttgtatta tattattttt agatggttca ttactcaatt caacgttcta acgccagggc
     9481 gaggagaaga agtagattct aaagaaattt ctgaatccgc agattcaact tcaaatactg
     9541 cagattattt aaaacaggat agcctacaaa ttatcagagc cttgggtgga tcaaataata
     9601 tagaagatgt agatgcttgt gtgacacgtt tacgtgtagc tgtaaaagaa gttaatcaag
     9661 ttgataaagc acttttaaaa caaattggtg cagttgatgt cttagaagtg aagggtggca
     9721 ttcaagcaat ctatggagca aaagcaatct tatataaaaa tagtattaat gaaattttag
     9781 gtgtagatga ttaagtactt actgacttaa taaaaaacag aggagagtga tggatgagta
     9841 ggatgaaatg aaatcgcata caagaaataa agaactcatt atccaagttg gatacgctta
     9901 ttacatagga gaatacaaat gaaatttaga aaattagctt gtacagtact tgcgggtgct
     9961 gcggttcttg gtcttgctgc ttgtggcaat tctggcggaa gtaaagatgc tgccaaatca
    10021 ggtggtgacg gtgccaaaac agaaatcact tggtgggcat tcccagtatt tacccaagaa
    10081 aaaactggtg acggtgttgg aacttatgaa aaatcaatca tcgaagcgtt tgaaaaagca
    10141 aacccagata taaaagtgaa attggaaacc atcgacttca agtcaggtcc tgaaaaaatc
    10201 acaacagcca tcgaagcagg aacagctcca gacgtactct ttgatgcacc aggacgtatc
    10261 atccaatacg gtaaaaacgg taaattggct gagttgaatg acctcttcac agatgaattt
    10321 gttaaagatg tcaacaatga aaacatcgta caagcaagta aagctggaga caaggcttat
    10381 atgtatccga ttagttctgc cccattctac atggcaatga acaagaaaat gttagaagat
    10441 gctggagtag caaaccttgt aaaagaaggt tggacaactg atgattttga aaaagtattg
    10501 aaagcactta aagacaaggg ttacacacca ggttcattgt tcagttctgg tcaaggggga
    10561 gaccaaggaa cacgtgcctt tatctctaac ctttatagcg gttctgtaac agatgaaaaa
    10621 gttagcaaat atacaactga tgatcctaaa ttcgtcaaag gtcttgaaaa agcaactagc
    10681 tggattaaag acaatttgat caataatggt tcacaatttg acggtggggc agatatccaa
    10741 aactttgcca acggtcaaac atcttacaca atcctttggg caccagctca aaatggtatc
    10801 caagctaaac ttttagaagc aagtaaggta gaagtggtag aagtaccatt cccatcagac
    10861 gaaggtaagc cagctcttga gtaccttgta aacgggtttg cagtattcaa caataaagac
    10921 gacaagaaag tcgctgcatc taagaaattc atccagttta tcgcagatga caaggagtgg
    10981 ggacctaaag acgtagttcg tacaggtgct ttcccagtcc gtacttcatt tggaaaactt
    11041 tatgaagaca aacgcatgga aacaatcagc ggctggactc aatactactc accatactac
    11101 aacactattg atggatttgc tgaaatgaga acactttggt tcccaatgtt gcaatctgta
    11161 tcaaatggtg acgaaaaacc agcagatgct ttgaaagcct tcactgaaaa agcgaacgaa
    11221 acaatcaaaa aagctatgaa acaatagtcc ttagttattc tataaaaagt agttttttaa
    11281 agaacctaag agtgtatacc cccttttccc tctacacaga tagtgtaaga aaagggggct
    11341 tttgtttaaa atgtaagaaa ctgtcacgaa attaaaatga agttcttaca taagcgaatc
    11401 ataaaaaatt tcattttgat tttaaaacag ttcaagaaag tcaaaaaatt attctatttg
    11461 aaagagaggt gccgactgtg aaagtcaata aaatccgtat gcgggaaaca gtgatttcct
    11521 acgctttcct agcaccagta ttattcttct ttgtcatctt tgtgttggct ccgatggtga
    11581 tgggcttcat tacaagtttc tttaactact caatgactaa atttgagttt gtaggcttgg
    11641 ataactatat ccgtatgttt aaagatcctg tctttacaaa atctctgatt aacacagtta
    11701 ttttggttat tggatctgta ccagttgttg ttctattctc actctttgta gcatctcaga
    11761 cctatcatca aaatgtcatt gccagatcct tctaccgttt cgtcttcttc cttcctgttg
    11821 taacgggtag tgttgccgtg acagttgttt ggaaatggat ttatgaccca ctatcaggga
    11881 ttctaaactt tgtccttaag tccagccaca tcatcagcca aaacatttct tggttgggag
    11941 ataaaaactg ggcattgatg gcgattatga ttattctctt gaccacttca gttggtcagc
    12001 ccatcatcct ttatatcgct gccatgggga atattgacaa ttcactggtt gaagcggcgc
    12061 gtgttgatgg tgcaactgag tttcaagttt tttggaagat taaatggcca agccttcttc
    12121 caacaactct ttatattgca atcatcacaa caattaactc attccagtgt ttcgccttga
    12181 ttcagctttt gacatctggt ggtccaaact actcaacaag taccttgatg tactaccttt
    12241 acgaaaaagc cttccaattg acagaatacg gctatgccaa cacaattggt gtcttcttgg
    12301 cagtcatgat tgctatcgta agctttgttc aatttaaagt acttggaaac gacgtagaat
    12361 actaaagaaa ggagacagct atgcaatcta cagaaaaaaa accattaaca gcctttactg
    12421 ttatttcaac aatcattttg ctcttgttga ctgtgctgtt catctttcca ttctactgga
    12481 ttttgacagg ggcattcaaa tcacaacctg atacaattgt tattcctcct cagtggttcc
    12541 ctaaaatgcc aaccatggaa aacttccaac aactcatggt gcagaaccct gccttgcaat
    12601 ggatgtggaa ctcagtattt atctcattgg taaccatgtt cttagtttgt gcaacctcat
    12661 ctctagcagg ttatgtattg gctaaaaaac gtttctatgg tcaacgcatt ctatttgcta
    12721 tctttatcgc tgctatggcg cttccaaaac aagttgtcct tgtaccattg gtacgtatcg
    12781 tcaacttcat gggaatccat gatactctct gggcagttat cttgcctttg attggatggc
    12841 cattcggtgt cttcctcatg aaacagttca gtgaaaatat ccctacagag ttgcttgaat
    12901 cagctaaaat cgacggttgt ggtgagattc gtaccttctg gagtgtagcc ttcccgattg
    12961 tgaaaccagg gtttgcagcc cttgcaatct ttaccttcat caatacttgg aatgactact
    13021 tcatgcaatt ggtaatgttg acttcacgta acaatttgac catctcactt ggggttgcga
    13081 ccatgcaggc tgaaatggca accaactatg gtttgattat ggcaggagct gcccttgctg
    13141 ctgttccaat cgtcacagtc ttcctagtct tccaaaaatc cttcacacag ggtattacta
    13201 tgggagcggt caaaggataa tactctgcga aaatctcttc aaactacgtc agcttcacct
    13261 tgccatactt aagtattgcc tgcggttagc ttcctagttt gttcttcaat tttcattgag
    13321 tataggaaaa tcaatctatc aagatacaga agtatatttt atagatttag agaatataga
    13381 ggttataagt gtctacaaaa tggagggtat gcagttactt tatgaagttt tgtcagacac
    13441 ttataaactt aagaatggtt ttagttaact atcagaaacg aaggaaagag tatgattttt
    13501 gacgatttga aaaacatcac cttttacaaa gggattcatc ctaatttaga caaggctatc
    13561 gactatctct accaacatcg taaggattct ttcgaattag gaaagtatga tattgatgga
    13621 gataaagtct ttctagttgt tcaggaaaat gtcctcaatc aagctgaaaa tgatcaattt
    13681 gagtatcata agaactatgc agatttgcat ttgctggtag aaggacatga atattcgagc
    13741 tacggttcac gtatcaaaga cgaggcagta gcattcgacg aagcgagtga cattggcttt
    13801 gttcattgtc atgaacacta cccactcttg ttgggttatc acaattttgc gattttcttc
    13861 ccaggtgagc cacatcagcc aaatggttat gcaggcatgg aagaaaaggt tcgaaaatat
    13921 ctctttaaaa ttttgattga ttaaaaatag gatgaattgt ttttttgtaa agctttgata
    13981 atactctacc atgaaattga tctttgtgag gtagagaaat gagaataaaa tatttaaaaa
    14041 ttggtatctt ctaagtatgc tgcaagagct agtttcttag atggacaggg gattacagtt
    14101 gatgagatgg cttggataat taggggcatt gtgaatgcat tgattggtag atacataaaa
    14161 ttaggtactt atgcggctaa gtatggtatt agtatggcac gctcgatctt aagtagggta
    14221 gctgcaactg cagcagcaag agtaggatta ctgaccaaga tttctggatg gattttacga
    14281 gtagctgtga atgtagctga tgtatatggt aattttgcca acaatattgc tgcagcttgg
    14341 gatgtatatg ataaaattcc taacaatggt cgtataaact tttaaaatgc gagaatgaaa
    14401 gcactttgta tttttttatt gaatatgtta gcttggacag tgcttgcaat gataattcgt
    14461 ggagggctag atggatttga taggcatact tggagtacta ttttaattgc ttcgctgttc
    14521 ggggtatatg attataagcc catagataaa aatagaaaaa agtccaaaag aaaaaataga
    14581 tttgttcatg gtagggactt atgaaagctt tactgacaaa aaagaaaaca gtttacaaag
    14641 aaaaatgatg gaggagcaaa catggcacaa aaaggagtaa gccttatcaa ggcagcattt
    14701 gatacagata actttctcat gcgttttagt gagaaggtct tggacatcgt gacagccaat
    14761 cttctttttg tcgtctcttg tttacccatc gtgacgattg gagtggctaa aatcagcctc
    14821 tacgagacca tgttcgaagt taagaagagc agacgggtgc ctgtttttaa aatctatcta
    14881 agatctttca agcaaaatct gaaactaggt cttcagctgg gtttaatgga gttaggaatt
    14941 gtgtttctta ccctttcaga tctctatctt ttctggggtc aaacagctct gcccttccaa
    15001 ttgctgaaag ccatttgttt aggtattctg atttttctta ctatcgtgat gctggctagt
    15061 taccctatcg cggcacgtta tgacctatct tggaaagaaa ttcttcaaaa aggattgatg
    15121 ttggctagtt ttaactttcc ttggttcttc ctcatgttag ccattcttgt cctcattgtg
    15181 atggttcttt atctgtccgc cttcagtcta ctcttaggtg gctcagtctt cctacttttt
    15241 gggtttggac tattggtctt tatccagact ggattgatgg agaaaatttt cgcaaaatac
    15301 caataggagc tttatttctg aaactacttt caaaggctcc aaacgctatt ctataagcga
    15361 gaaactaaaa tctaagtgtc tataagatat tgaaagcctt ttcggtaaag tgtatactaa
    15421 agaagtaaag aaaaatctgc tttagcagaa aataaaaata ataggagaaa atctatgtca
    15481 gatttgaaaa aatacgaagg tgtcattcca gccttctacg catgttatga tgatcaagga
    15541 gaagtaagcc cagaacgtac gcgtgccttg gttcaatact tcattgataa aggtgttcaa
    15601 ggtctttatg tcaatggttc ttctggtgaa tgtatctacc aaagcgttga agatcgcaag
    15661 ttgattttgg aagaagtcat ggcggtagcc aaaggtaaat tgaccattat tgcccatgtt
    15721 gcttgcaata atactaaaga tagtatggaa cttgctcgcc atgctgaaag cttgggagta
    15781 gatgctattg caacgattcc accaatttat ttccgcttgc cagaatactc agttgccaaa
    15841 tactggaacg atatcagttc tgcagctcca aacacagact acgtgattta caacattcct
    15901 caattggcag gggttgcttt gactccaagc ctttacacag aaatgttgaa aaatcctcgt
    15961 gttatcggtg tgaagaactc ttctatgcca gttcaagata tccaaacctt tgtcagcctt
    16021 ggtggagaag accatatcgt ctttaatggt cctgatgagc agttcctagg aggacgcctc
    16081 atgggggcta gggctggtat cggtggtact tatggtgcta tgccagaact cttcttgaaa
    16141 ctcaatcagt tgattgcgga taaggaccta gaaacagcgc gtgaattgca gtatgctatc
    16201 aacgcaatca ttggtaaact cacttctgct catggaaata tgtacggtgt catcaaagaa
    16261 gtcttgaaaa tcaatgaagg cttgaatatt ggatctgttc gttcaccatt gacaccagtg
    16321 actgaagaag atcgtccagt tgtagaagcg gctgctgcct tgattcgtga aaccaaggag
    16381 cgcttcctct aatctaaaag gaggtattta tgacatatta cgttgcaatt gatatcggtg
    16441 gaaccaacat caagtatggt ttggttgatc aagaggggca acttcttgaa tcgcatgaaa
    16501 tgccaactga ggcgcataag ggtggacctc atatcttaca aaagaccaaa gatatcgtag
    16561 ctagttattt agaaaaaggc ccagtagcag gtgttgccat atcttctgct gggatggtgg
    16621 atccggataa gggtgagatt ttctatgctg ggccgcaaat ccctaactac gcaggcaccc
    16681 agttcaaaaa ggaaatcgaa gaaagcttta ctattccttg tgagattgaa aatgatgtca
    16741 actgtgcagg tcttgctgag gcagtatctg gttcaggcaa gggagcaagt gtgacacttt
    16801 gcttgaccat tggaaccggt atcggtggtt gcttgattat ggataggaaa gtcttccatg
    16861 gttttagcaa ttcagcctgt gaagtcgggt atatgcatat gcaggatgga gcttttcaag
    16921 acttggcttc tacaacagct ttagtgaaat atgtagctga agcccatgga gaagatgttg
    16981 atcagtggaa tggccgtaga attttcaaag aagccactga aggaaacaaa atctgcatgg
    17041 aaggtattga ccgtatggtt gactatctag gaaaaggtct ggcaaatatt tgctacgttg
    17101 ccaatccaga agtggttatt cttggtggtg gtatcatggg gcaagaggct atcctcaaac
    17161 ctaagatccg tacagccttg aaagaggctt tggtaccaag tttagcagaa aaaacacgat
    17221 tagaatttgc ccatcaccaa aatacagcag ggatgttggg tgcatattat cattttaaga
    17281 caaaacaatc ctagtttggc tcagccaaac taggattttc ttacacgttt ttgtctacga
    17341 tagccgttga gttttttatt ttcccagtag ctattaaaga ttttttcctt gctttcgcga
    17401 ttgatttcca aaaagtaggc ataaatcaaa tcgataaaga agagcatagg aagttgagcg
    17461 gatattcgtt ggatatagga gggttggctg tgggtggcta caagaacagt ctctgtatag
    17521 gtctggctat ctttattggg aacacttgta aagagtacag tctttgcccc catctcctta
    17581 gcatctaata gactatctaa aatagaagga gttgagcctg aaagtgagaa gcccagtact
    17641 agacaatttt catccatgat gctggttgtc caggcaaagc cgtcttggtc tgtcaaagct
    17701 tcgcagacca cacctagtcg cataaaacgt aatttcattt cacgggcgac gaggccagaa
    17761 ctccctgttc caaagaagta gatacgctca gcatcttcga ttagctgggc aattcgttct
    17821 agttggattt cgtcaatcaa gtcttgtgtt tgttccctca tattgctata acttctgagg
    17881 actcgtttgg tcagtggact gtgcttggag acttggttgg cttgattttc tgcctgatgt
    17941 tggtattgga aaataaattc tcggtagcca gtaaagccac actttttagc aaagcgggtc
    18001 aaagcagctt gagaaatatg taatttttgg gtgacttgtt gagaagataa atcatctgta
    18061 atcgtttcag cttgcaaaaa atagcgagcg atttcttgtt ctaggtctgt catttcttca
    18121 aaatgtgaat caatgatagt tgcgatatct ggtttgtcca tagggaaagc tcctttacat
    18181 gagtcatact ggaagactag atcagagaat agtcacactt cattataaca cataatataa
    18241 ggatagataa ataaaaacgc atctctgttt taaaaacgaa aaaatcgaaa aagcttctct
    18301 cttttccata attttctact caaattgtgg tacaattaag agtaagattt taagttagaa
    18361 atgagactga tttgtatgag aaaatttaac agccattcga ttccgattcg gcttaattta
    18421 ttgttttcaa tcgtcatttt actctttatg accattattg gtcgtttgtt gtatatgcag
    18481 gttttgaaca aggattttta cgaaaaaaag ctagcttcag ctagtcagac caagattaca
    18541 agcagttcag cccgtgggga aatttatgat gctagtggaa aacctttggt agaaaatacg
    18601 ttaaagcagg ttgtttcctt tacgcgtagc aataaaatga cggctacaga cttaaaagaa
    18661 acagctaaaa agttactgac ttatgtgagc atcagttctc caaatttgac agaacgccag
    18721 ctggcggatt actatttggc tgatcctgaa atctataaaa aaatagtgga agctctccca
    18781 agtgagaaac gcttggattc agatggcaat cgtctatccg aatcagaact gtataacaat
    18841 gcggtcgata gtgtacaaac gagtcaacta aactatacag aggatgaaaa gaaagaaatc
    18901 tatcttttta gtcagttaaa tgctgttgga aactttgcga caggaaccat tgcgacagat
    18961 cctctaaatg attctcaggt ggctgttatt gcctctattt caaaggagat gcctggcatt
    19021 agtatttcta cttcttggga tagaaaggtt ttggaaactt ccctttcttc tatagttggg
    19081 agtgtatcca gtgaaaaagc tggtctccca gcggaagaag cagaagccta tcttaaaaaa
    19141 ggctattctc taaatgaccg tgtaggaacc tcctatttgg aaaagcaata tgaagagacc
    19201 ttacaaggaa aacgctcggt aaaagaaatc catctggata aatatggcaa tatggaaagc
    19261 gtggatacaa ttgaggaagg tagtaaggga aacaatatca aactgaccat tgatttggct
    19321 ttccaagata gcgtggatgc tttactgaaa agttatttca attctgagct agaaaatggt
    19381 ggagccaagt attctgaagg tgtctatgca gtcgccctta acccaaaaac aggtgcggtt
    19441 ttgtctacgt cagggattaa acatgacttg aaaacgggag agttgacgcc tgattccttg
    19501 ggaacggtaa ccaatgtctt tgttccaggt tcggttgtca aggcggcgac catcagctca
    19561 ggttgggaaa atggagtctt gtcaggaaac cagaccttga cagaccagtc cattgtcttc
    19621 caaggttcag ctcccatcaa ttcttggtat actcaggctt acggttcatt ccctatcaca
    19681 gcggtccaag ctctggagta ttcatcaaat acctatatgg tccaaacagc cttaggtctt
    19741 atggggcaaa cctatcaacc caatatgttt gtcggcacca gcaatctaga gtctgctatg
    19801 gagaaactgc gttcaacctt tggcgaatat ggcttgggta ctgcgacagg aattgaccta
    19861 ccagatgaat ctactggatt tgttcccaaa gagtatagct ttgctaatta cattactaat
    19921 gcctttgggc agtttgataa ctatacgccg atgcagttgg ctcagtatgt agcaactatt
    19981 gcaaataatg gtgttcgtgt ggctcctcgt attgttgaag gcatttatgg taataatgat
    20041 aagggaggac tgggtgactt gattcagcaa ctgcaaccga cagagatgaa taaggtcaat
    20101 atatccgact ccgatatgag catcttgcac caaggttttt atcaggttgc ccatggtact
    20161 agtggattga caactggacg tgccttttca aatggtgcct tggtatccat tagcggaaaa
    20221 acaggtacag ccgaaagcta tgtggcagat ggtcagcaag caaccaatac caatgcggtg
    20281 gcctatgccc catctgataa tccccaaatc gctgtcgcag tggtctttcc tcataatacc
    20341 aatctaacaa atggtgtagg accttccatt gcgcgtgaca ttatcaatct gtatcaaaaa
    20401 taccatccaa tgaattagaa aggaaattat gctttatcca acacctattg ccaagttgat
    20461 tgacagttat tctaagttac caggtatcgg gattaagacg gctacgcgtc tggcctttta
    20521 tacgattggg atgtctgctg atgatgtcaa tgaatttgca aaaaatctcc tttctgctaa
    20581 gagagaattg acatattgtt ctatttgtgg acgtttgaca gacgacgatc cttgttctat
    20641 ctgtactgat ccgactcgtg accagacaac aattttagtt cttgaggata gtagagatgt
    20701 ggcagccatg gaaaatatcc aagaatacca tggactctat catgtccttc atggcctcat
    20761 ttctcctatg aatggtatca gtccggacga tatcaatctc aagagcctta tgactcgtct
    20821 tatggatagt gaggtttcag aagtgattgt ggcgactaat gctacagcgg atggtgaagc
    20881 gacttccatg tatctttcac gtttgctcaa gccggctggt atcaaggtta cgcgtctagc
    20941 acgaggtctc gctgtgggag cggacattga gtatgcggac gaagtgacac tcttacgagc
    21001 cattgaaaat cggacagagt tgtaagtgta ggcaaattta cgaactccat tcatttataa
    21061 aaaatcaaag aggctgaaaa tcgttcctat cggcctcttt ttgtatagtg tgatgagtag
    21121 gctcaggttc aagttttaaa aaaccaagca aatatgatat actaaagagc gagtattcta
    21181 gtagaattag gacaaataat atgaaacaaa cgattattct tttatatggt ggacggagtg
    21241 cggaacgcga agtctctgtc ctttcagctg agagtgtcat gcgtgcggtc gattacgacc
    21301 gtttcacagt caagactttc tttatcagtc agtcaggtga ctttatcaaa acacaggaat
    21361 ttagtcatgc tccggggcaa gaagaccgtc tcatgaccaa tgaaaccatt gattgggata
    21421 agaaagttgc accaagtgct atctacgaag aaggtgcagt ggtctttcca gtccttcacg
    21481 ggccaatggg agaagatggc tctgttcaag gattcttgga agttttgaaa atgccttacg
    21541 ttggttgcaa cattttgtca tcaagtcttg ccatggataa aatcacgact aagcgtgttc
    21601 tggaatctgc tggtattgcc caagttcctt atgtggctat cgttgaaggc gatgatgtga
    21661 ctgctaaaat cgctgaagtg gaagaaaaat tggcttatcc agtcttcact aagccgtcaa
    21721 acatggggtc tagtgtcggt atttctaagt ctgaaaacca agaagaactc cgtcaagcct
    21781 taaaacttgc cttccgatat gacagccgtg tcttggttga gcaaggagtg aatgcccgtg
    21841 aaattgaggt tggcctcttg ggtaactacg atgtcaagag cacgctacca ggagaagttg
    21901 tcaaggacgt tgccttttat gactacgatg ccaagtatat tgataacaat attactatgg
    21961 atattcctgc caaaatcagt gatgatgtgg tggctgtcat gcgtcaaaat gcagaaacag
    22021 ccttccgtgc cattggtggc cttggtctat ctcgttgcga tttcttctat acagataagg
    22081 gagagatttt tctcaacgag ctcaatacta tgccaggttt cacccagtgg tctatgtacc
    22141 cactactttg ggacaatatg gggatcagct acccaaaact aatcgagcgt ttggttgacc
    22201 ttgccaagga aagttttgac aagcgcgaag cgcatttgat ataaaatgaa agagagggta
    22261 gaagccagaa ccatcactgc acggtgacta gagttctcgg acttcagccc tttttaaagg
    22321 agtagaaatg aaattaacaa tccatgaaat tgcccaagtt gttggagcca aaaatgatat
    22381 cagtatcttt gaggacaccc agttagaaaa agctgagttt gatagtcgtt tgattggaac
    22441 tggagattta tttgtgccac ttaaaggtgc gcgtgatggc catgacttta ttgaaacagc
    22501 ctttgaaaat ggtgcagcag taaccttgtc tgagaaagag gtctcaaatc atccttacat
    22561 tctagtagat gatgttttga cagcctttca atccttagca tcctactatc ttgaaaaaac
    22621 gactgttgat gtctttgctg ttacaggttc aaatggcaag acaacgacta aggatatgtt
    22681 ggcgcattta ctgtcaacaa gatacaagac ctacaaaaca caaggcaatt acaataatga
    22741 gattggcctt ccttacacag ttcttcatat gcctgaagga acagaaaagt tggttttgga
    22801 gatgggacag gatcacttgg gcgatattca tctcttgtct gaattggctc gtccaaaaac
    22861 agccatcgtg accttggttg gagaagccca tttggccttt ttcaaagacc gttcagagat
    22921 tgctaaggga aaaatgcaaa ttgcagacgg aatggcttca ggttccttgc ttttagcgcc
    22981 ggctgaccct atcgtagagg actatttgcc aactgataaa aaggtggttc gttttgggca
    23041 aggggcagag ctggaaatta ctgacttggt tgagcgcaaa gatagtctga ccttcaaggc
    23101 caatttctta gagcaagccc ttgatttgcc agtaactggc aagtacaatg cgacaaatgc
    23161 tatgattgca tcctatgttg ccttgcaaga aggagtttca gaggagcaaa ttcgtttggc
    23221 cttccaagat cttgaattga cgcgtaaccg taccgagtgg aagaaagcag ccaatggagc
    23281 agatatcctg tcagatgttt acaatgccaa tccaactgct atgaaactga ttttagagac
    23341 tttctctgcc attccagcca atgaaggtgg caagaaaatt gcagtgttgg cggatatgaa
    23401 ggagcttggt gaccagtctg ttcaacttca taatcagatg attttgagcc tttctccaga
    23461 tgtgcttgat accgtgattt tctatggaga aaatattgct gaattagccc aattggccag
    23521 tcaaatgttc ccaatcggcc acgtttacta cttcaagaaa acagaagacc aggatcaatt
    23581 tgaagaccta gtcaagcagg tcaaggaaag ccttggagcc catgaccaaa tcctgctcaa
    23641 aggctctaac tctatgaatc tagccaagtt ggtagaaagt ttagaaaatg aagacaagtg
    23701 attttgtcaa gtatttgcaa agaatgattg ccattacaga tactggctta acctttacaa
    23761 aagatccgtt tgaccgtgag cgctacgaag acttgcgaag tctgttatct gaaatgttga
    23821 atcaagcatc agaccttgat tccgaagaag tggcagaagt cttgaagcca acttctgctt
    23881 atgcgactcc gttaatggac gtccgtgctt ggattgttga ggatgagaag atttgtctgg
    23941 ttaggggaca aggagaggat agttgggctt tgccaggtgg ctttggtgaa gtcggctatt
    24001 ctccaacaga aaatattctc aaggaaattg aagaagaaac cggttttaaa gccaaagttg
    24061 aaagactgct agctgttttt gataccaatc gtttccaact acagagcaaa cagtatacaa
    24121 agtttgtttt tggatgtaag cttcttgatg gacaattcca agaaaatcaa gaaattgctg
    24181 accttcaatt ttttgccatt gaccaactgc cgaacttatc tgaaaaacgc attaccaagg
    24241 agcaaataga gcttctttgg caggtttatc aaggtcatag ggggcaatat cttgactaag
    24301 aagatgatta tcgtatttct aaatccattt ttaacaacta gcatggtata ataatatgca
    24361 ggaaaatttt gaattatgag gaagactaga tgaatttatg ggatattttc tttacgactc
    24421 aggcaaccga gccgcccaaa tttgaccttt tttggtatgt tagcctattt acgctcttag
    24481 ccttaacctt ttatacagcc catcgctatc gtgaaaagaa ggtttaccaa cgatttttcc
    24541 aaatcttgca gactgttcag ttaatccttc tttatggttg gtactgggtc aatcatatgc
    24601 cactgtcaga aagcctaccc ttttaccatt gccgtatggc tatgtttgtg gtactcttgc
    24661 ttcctggtca atccaaatat aaacaatact ttgcattatt gggaacattt gggacattag
    24721 cagcctttgt ttatccagtg ccagatgctt acccttttcc acatatcacc attctatcct
    24781 ttatctttgg tcatttagca ctcttgggga actctctagt ttatctattg agacagtata
    24841 atgcgcgatt gctggatgtg aagggaattt ttctcatgac ctttgcccta aatgccttga
    24901 tttttgtggt caatttggtg acaggtggcg attacggatt tttgacaaaa ccgccattgg
    24961 ttggggatca cggtctagta gctaattatt tacttgtttc aattgtgctg gtagctacta
    25021 tcagtttgac taagaaaatc ttagaattct ttttagctca agaagcagaa aaaatgattg
    25081 caaaggaagc ttaacacaga gctttctttt ttgctcttag agagttttta caagcagctt
    25141 ataaaataag aatttctgaa tagacaaact caaaaaatgg ctgggaaatt taggaaaaaa
    25201 gcaagcacga ttaaattttt tgtgttataa tattttgtga atagctatgc ctatgtttag
    25261 ctatggaata atacgaagtg cgaaacttgg aagatagaga ggaagcgatg taatggctag
    25321 agaaggcttt tttacaggtc tagatattgg aacaagctct gtcaaggtgc ttgtggccga
    25381 gcagagaaat ggtgaattaa atgtaattgg cgtgagtaat gccaaaagta aaggtgtaaa
    25441 ggatggaatt attgttgata ttgatgcagc agcaactgct atcaagtcag ccatttccca
    25501 agcggaagaa aaggcaggca tttcgattaa atcagtgaat gtcggcttgc ctggtaatct
    25561 tttgcaggta gaaccaactc aggggatgat tccagtaaca tctgatacta aggaaattac
    25621 ggatcaagat gttgaaaatg ttgtcaaatc agctttgaca aagagtatga cacctgaccg
    25681 tgaagtcatt acctttattc ctgaagaatt tattgtggat ggtttccaag ggattcgtga
    25741 cccacgtggc atgatggggg ttcgccttga aatgcgtggt ttgctttata caggacctcg
    25801 tactatcttg cacaatttgc gtaagacggt tgagcgtgca ggtgttcagg ttgaaaatgt
    25861 tatcatttca ccactagcaa tggttcagtc tgttttgaac gaaggggaac gtgaatttgg
    25921 tgctacagtg attgatatgg gggcaggtca aacgactgtc gctacaatcc gtaatcaaga
    25981 actccagttc acacatattc tccaagaagg tggagattat gtaactaaag atatctccaa
    26041 ggttttgaaa acctctcgca aattagcgga aggcttgaaa ctgaattacg gggaagccta
    26101 tccgcctctt gcaagcaaag aaaccttcca agtagaggtt attggagaag tagaagcagt
    26161 cgaagtgacg gaagcctact tgtcagaaat tatttctgca cgaatcaagc acatccttga
    26221 acaaatcaag caagaattag atagaaggcg tctattggac ctccctggtg gtattgtctt
    26281 aatcggtggg aatgccattt taccaggtat ggttgagctt gctcaggaag tctttggcgt
    26341 ccgtgtcaag ctttatgttc caaatcaagt tggtatccgt aatccagcct ttgcgcatgt
    26401 gattagttta tcagaatttg cgggtcaatt aacagaagtt aatcttttgg ctcagggagc
    26461 gataaaaggt gagaatgact taagtcatca gccaattagt tttggtggga tgctgcaaaa
    26521 aacagctcag tttgtacaat caacgcctgt tcaaccagct cctgctccag aagtagagcc
    26581 ggtggcgcct acagaaccaa tggcggattt ccaacaagct tcacaaaata aaccgaaatt
    26641 agcagatcgt ttccgtggat tgatcggaag catgtttgac gaataaagag gaaaaataaa
    26701 ttatgacatt ttcatttgat acagctgctg ctcaaggggc agtgattaaa gtaattggtg
    26761 tcggtggagg tggtggcaat gccatcaacc gtatggtcga cgaaggtgtt acaggcgtag
    26821 aatttatcgc agcaaacaca gatgtacaag cattgagtag tacaaaagct gagactgtta
    26881 ttcagttggg acctaaattg actcgtggtt tgggtgcagg aggtcaacct gaggttggtc
    26941 gtaaagccgc tgaagaaagc gaagaaacac tgacggaagc tattagtggt gccgatatgg
    27001 tcttcatcac tgctggtatg ggaggaggct ctggaactgg agctgctcct gttattgctc
    27061 gtatcgccaa agatttaggt gcgcttacag ttggtgttgt aacacgtccc tttggttttg
    27121 aaggaagtaa gcgtggacaa tttgctgtag aaggaatcaa tcaacttcgt gagcatgtag
    27181 acactctatt gattatctca aacaacaatt tgcttgaaat tgttgataag aaaacaccgc
    27241 ttttggaggc tcttagcgaa gcggataacg ttcttcgtca aggtgttcaa gggattaccg
    27301 atttgattac caatccagga ttgattaacc ttgactttgc cgatgtgaaa acggtaatgg
    27361 caaacaaagg gaatgctctt atgggtattg gtatcggtag tggagaagaa cgtgtggtag
    27421 aagcggcacg taaggcaatc tattcaccac ttcttgaaac aactattgac ggtgctgagg
    27481 atgttatcgt caacgttact ggtggtcttg acttaacctt gattgaggca gaagaggctt
    27541 cacaaattgt gaaccaggca gcaggtcaag gagtgaacat ctggctcggt acttcaattg
    27601 atgaaagtat gcgtgatgaa attcgtgtaa cagttgttgc aacgggtgtt cgtcaagacc
    27661 gcgtagaaaa ggttgtggct ccacaagcta gatctgctac taactaccgt gagacagtga
    27721 aaccagctca ttcacatggc tttgatcgtc attttgatat ggcagaaaca gttgaattgc
    27781 caaaacaaaa tccacgtcgt ttggaaccaa ctcaggcatc tgcttttggt gattgggatc
    27841 ttcgccgtga atcgattgtt cgtacaacag attcagtcgt ttctccagtc gagcgctttg
    27901 aagccccaat ttcacaagat gaagatgaat tggatacacc tccatttttc aaaaatcgtt
    27961 aagtaaatga atgtaaaaga aaatacagaa cttgtttttc gagaagttgc agaggctagt
    28021 ctgagtgctc atcgagagag tggttcggtc tctgtcattg cagttaccaa gtatgtagat
    28081 gtaccgacag cggaagcctt gcttccgcta ggtgtccatc atatcggtga aaatcgtgta
    28141 gataagtttc tggaaaaata tgaagcttta aaagatcgag atgtgacttg gcatttgatt
    28201 ggtaccttgc aaagacgtaa ggtgaaagat gtcattcaat acgttgatta tttccatgca
    28261 ttggactcag taaagctagc aggggaaatt caaaaaagaa gtgaccgagt catcaagtgt
    28321 ttccttcaag taaatatttc taaagaagaa agcaaacacg gtttttcgag agaggaactg
    28381 ctggaaatct tgccagagtt agccagacta gataagattg aatatgttgg tttaatgacg
    28441 atggcacctt ttgaggctag cagtgagcag ttgaaagaga ttttcaaggc ggcccaagat
    28501 ttacaaagag aaattcaaga gaaacaaatt ccaaatatgc ctatgaccga gttaagtatg
    28561 ggaatgagtc gtgattataa agaagcgatt caattcggtt ccacttttgt tcgtataggt
    28621 acatcatttt ttaagtagga gagaaccatg tctttaaaag atagattcga tagatttata
    28681 gattatttta cggaggatga ggattcaagt ctcccttatg aaaaaagaga tgagcctgtg
    28741 tttacttcag taaattcttc acaggaaccg gctctcccaa tgaatcaacc ttcacagtcg
    28801 gctggcacaa aagagaacaa tatcaccaga cttcatgcaa gacaacagga attggcaaat
    28861 cagagtcagc gtgcaacgga taaggtcatt atagatgttc gttatcctag aaaatatgag
    28921 gatgcaacag aaattgttga tttattggca ggaaacgaaa gtatcttgat tgattttcag
    28981 tatatgacag aggtgcaggc tcgtcgttgt ttggactatt tggatggagc ttgtcatgtt
    29041 ttagctggaa atttgaaaaa ggtagcttct accatgtatt tgttgacacc agtgaacgtt
    29101 attgtaaatg ttgaagatat ccgtttacca gatgaagatc aacagggtga gttcggtttt
    29161 gatatgaagc gaaatagagt acgataatga tttttttaat tcgtatgatt tataatgcag
    29221 tggatattta ctccctgatt ttggtagcct tcgctgtcat gtcttggttt ccaggtgcct
    29281 acgaatccag tttaggtcgt tggattgtag cgttggtgaa accagtgctt gctcccttgc
    29341 aacgcctgcc tttacagata gcgggtcttg atttatctgt ttgggttgcg attgttttgg
    29401 ttcgattttt aggagaaaac ctagtgcgtt ttctggcgat gataggatga ataaagggat
    29461 ttatcagcat ttctccatag aagatcgtcc atttcttgac aagggaatgg aatggataaa
    29521 gaaggtagaa gatagctatg ctcctttttt aactcctttt atcaatcctc atcaggagaa
    29581 gctattaaag attttggcca aaacctatgg tcttgcttgt agcagtagtg gggaattcgt
    29641 ctcgagtgag tatgttcgag ttttattata cccagattat ttccaaccag agttttcaga
    29701 ttttgaaata tctctccagg aaattgtgta ttccaataaa tttgaacatt taacgcatgc
    29761 taagatttta gggacagtca tcaatcaatt agggattgaa cggaaacttt ttggagatat
    29821 cctagtagat gaagaacggg cgcagattat gattaatcag cagtttcttc ttctctttca
    29881 agatggacta aagaaaattg gtcgtatacc tgtttcgctg gaggaacgtc ctttcaccga
    29941 gaaaatagat aagctagaac agtatcgaga actggattta tctgtgtcta gttttcgatt
    30001 agatgttctt ttatcaaatg ttttgaaact atctaggaat caagcaaacc agttgattga
    30061 aaagaaactt gtccaagtaa attatcatgt ggtagacaaa tcagattaca ctgttcaagt
    30121 tggagacttg attagtgtga gaaaatttgg tcgcttgaga ttacttcaag ataagggaca
    30181 aacgaaaaaa gagaagaaaa aaataaccgt ccagttatta ttaagtaagt gaggaataga
    30241 atgccaatta catcattaga aataaaggac aagacttttg gaactcgatt cagaggtttt
    30301 gatccagaag aagtcgatga atttttagat attgtggttc gtgattacga agatcttgtg
    30361 cgtgcgaatc atgataaaaa tttgcgtatt aagagtttag aagagcgttt gtcttacttt
    30421 gatgaaataa aagattcatt gagccagtct gtattgattg ctcaggatac agctgagaga
    30481 gtgaaacagg cggcgcatga acgttcaaac aatatcattc atcaagcaga gcaagatgcg
    30541 caacgcttgt tggaagaagc taaatataag gcaaacgaga ttcttcgtca agcaactgat
    30601 aatgctaaga aagtcgctgt tgaaacagaa gaattgaaga acaagagccg tgtcttccac
    30661 caacgtctca aatctacaat tgagagtcag ttggctattg ttgaatcttc agattgggaa
    30721 gatattctcc gtccaacagc tacttatctt caaaccagtg atgaagcctt taaagaagtg
    30781 gttagcgaag tacttggaga accgattcca gctccaattg aagaagaacc aattgatatg
    30841 acacgtcagt tctctcaagc agaaatggca gaattacaag ctcgtattga ggtagccgat
    30901 aaagaattgt ctgaatttga agctcagatt aaacaggaag tggaagctcc aactcctgta
    30961 gtgagtcctc aagttgaaga agagcctctg ctcatccagt tggcccaatg tatgaagaac
    31021 cagaagtagc tccaatgcat ccgataggtc caacaccagc tacagaaact gttgattcaa
    31081 taccgggatt tgaagcaccg caagaatctg ttacaatttt ataagaaata ttctgagaac
    31141 aatatcttat ccttatattt ccagcgagca ggagatggtg tgagtcctgt aatccctatt
    31201 gataagatta tcctctcaaa aactcaagtc tgaagctagt aagatttgac gtttcccacg
    31261 ttacgggata agagggagaa agactaaatc tttttccgaa taaaggtggt accacgattt
    31321 tcgtcctttt tggaagtcgt ggtttttaat ttgttattat ttataaagga gataccatga
    31381 aactcaaaga cacccttaat cttgggaaaa ctgaattccc aatgcgtgca ggccttccta
    31441 ccaaagagcc agtttggcaa aaggaatggg aagatgcaaa actttatcaa cgtcgtcaag
    31501 aattgaacca aggaaaacct catttcacct tgcatgatgg ccctccatac gctaacggaa
    31561 atatccacgt tggacatgct atgaacaaga tttcaaaaga tatcattgtt cgttctaagt
    31621 ctatgtcagg attttacgca ccatttattc ctggttggga tactcatggt ctgccaatcg
    31681 agcaagtctt gtcaaaacaa ggtgtcaaac gtaaagaaat ggacttggtt gagtacttga
    31741 aactttgccg tgagtacgct ctttctcaag tagataaaca acgtgaagat tttaaacgtt
    31801 tgggtgtttc tggtgactgg gaaaatccat atgtgacctt gactcctgac tatgaagcag
    31861 ctcaaattcg tgtatttggt gagatggcta ataagggtta tatctaccgt ggtgctaagc
    31921 cagtttactg gtcatggtca tctgagtcag cacttgctga agcagagatt gaataccatg
    31981 acttggtttc aacttccctt tactatgcca acaaggtaaa agatggcaaa ggagttctag
    32041 atacagatac ttatatcgtt gtctggacaa cgactccatt taccatcaca gcttctcgtg
    32101 gtttgacggt tggtgcagat attgattacg ttttggttca acctgctggt gaagctcgta
    32161 agtttgtcgt tgctgctgaa ttattgacta gcttgtctga gaaatttggc tgggctgatg
    32221 ttcaagtttt ggaaacttac cgtggccaag aactcaacca catcgtaaca gaacacccat
    32281 gggatacagc tgtagaagag ttggtaattc ttggtgacca cgttacgact gactctggta
    32341 caggtattgt ccatacagcc cctggttttg gtgaggacga ttacaatgtt ggtattgcta
    32401 ataatcttga agtcgcagtg actgttgatg aacgtggtat catgatgaag aatgctggtc
    32461 ctgaatttga aggtcaattc tatgaaaagg tagttccaac tgttattgaa aaacttggta
    32521 acctccttct tgcccaagaa gaaatctctc actcatatcc atttgactgg cgtactaaga
    32581 aaccaatcat ctggcgtgca gttccacaat ggtttgcctc agtttctaaa ttccgtcaag
    32641 aaatcttgga cgaaattgaa aaagtgaaat tccactcaga atggggtaaa gtccgtcttt
    32701 acaatatgat ccgtgaccgt ggtgactggg ttatctctcg tcaacgtgct tggggtgttc
    32761 cacttcctat cttctacgct gaagatggta cagctatcat ggtagctgaa actattgaac
    32821 acgtagctca actttttgaa gaatatggtt caagcatttg gtgggaacgt gatgccaaag
    32881 acctcttgcc agaaggattt actcatccag gttcaccaaa cggcgagttc aaaaaagaaa
    32941 ctgatatcat ggacgtttgg tttgactcag gttcatcatg gaatggagtg gtggtaaacc
    33001 gtcctgaatt gacttaccca gccgaccttt acctagaagg ttctgaccaa taccgtggtt
    33061 ggtttaactc atcacttatc acatctgttg ccaaccatgg cgtagcacct tacaaacaaa
    33121 tcttgtcaca aggttttgcc cttgatggta aaggtgagaa gatgtctaaa tctcttggaa
    33181 atactattgc tccaagcgat gttgaaaaac aattcggtgc tgaaatcttg cgtctctggg
    33241 taacaagtgt tgactcaagc aatgacgtgc gtatctctat ggatatcttg agccaagttt
    33301 ctgaaactta ccgtaagatt cgtaacactc ttcgtttctt gattgccaat acatctgact
    33361 ttaacccagc tcaagataca gtcgcttacg atgagcttcg ttcagttgat aagtacatga
    33421 cgattcgctt taaccagctt gtcaagacca ttcgtgatgc ctatgcagac tttgaattct
    33481 tgacgatcta caaggccttg gtgaacttta tcaacgttga cttgtcagcc ttctaccttg
    33541 attttgccaa agatgttgtt tacattgaag gtgccaaatc actggaacgc cgccaaatgc
    33601 agactgtctt ctatgacatt cttgtcaaaa tcaccaaact cttgacacca atccttcctc
    33661 acactgcgga agaaatctgg tcatatcttg agtttgaaac agaagacttc gtccaattgt
    33721 cagaattacc agaagttcaa acttttgcta accaagaaga aatcttggat acatgggcag
    33781 ccttcatgga ctttcgtgga caagcacaaa aagccttgga agaagctcgt aatgcaaaag
    33841 ttatcggtaa atcacttgaa gcacacttga cagtttatcc aaatgaagtt gtgaaaactc
    33901 tactcgaagc agtaaacagc aatgtagcac aacttttgat cgtgtctgag ttgaccatcg
    33961 cagaaggacc agctccggaa gctgccctta gcttcgaaga tgtagccttc acagttgaac
    34021 gtgctactgg tgaagtatgt gaccgttgcc gtcgtatcga cccaacaaca gcagaacgca
    34081 gctaccaggc agttatctgt gaccactgtg caagcatcgt agaagaaaac tttgcggaag
    34141 cagtcgcaga aggatttgaa gagaaataag attgaaaagt ctaggcaaaa ttcaatttga
    34201 gaagaaaaga caactaattt tatagtctat taaacgcatt gtatcacgtt tttgaatacc
    34261 tgatatgatg cgttttttat ttattttaaa aatttgcgag gtatgacttt ttatactcaa
    34321 caagaatcaa agagaaactt agcaagctaa cagtagtaag ataaaatagg aatttgatat
    34381 tagggataag attggtaaat agtgt
//
LOCUS       DAVKNL010000019        33919 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVKNL010000019 DAVKNL010000000
VERSION     DAVKNL010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33919
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.019"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(88..324)
                     /locus_tag="VW411_000897"
     CDS             complement(88..324)
                     /locus_tag="VW411_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9793654.1"
                     /translation="MDVILSAIIFGISHLILSHRDPISLLYYSLIGFFFALVYRSTDN
                     LRLTILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(337..810)
                     /locus_tag="VW411_000898"
     CDS             complement(337..810)
                     /locus_tag="VW411_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793655.1"
                     /translation="MRNTKKLRQFGIFLLIILLSTYLPQTIGLYVTIILGLGADVYSL
                     ILTMGLVGSFLLLIWRLKKKKMLFIFEKKSWNWSFVFYLFATYVVYQILGNFWARYAH
                     LINHRNIHDEYFTVLFSNGQPTFLSTILSFVLPVIIGPVFEETLDRGYFMNTFFP"
     gene            complement(828..1013)
                     /gene="briC"
                     /locus_tag="VW411_000899"
     CDS             complement(828..1013)
                     /gene="briC"
                     /locus_tag="VW411_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HEU9793656.1"
                     /translation="MTGTNTFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRAG"
     gene            complement(1172..1939)
                     /locus_tag="VW411_000900"
     CDS             complement(1172..1939)
                     /locus_tag="VW411_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEU9793657.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEIGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(1936..2802)
                     /locus_tag="VW411_000901"
     CDS             complement(1936..2802)
                     /locus_tag="VW411_000901"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEU9793658.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(2839..4206)
                     /gene="accC"
                     /locus_tag="VW411_000902"
     CDS             complement(2839..4206)
                     /gene="accC"
                     /locus_tag="VW411_000902"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU9793659.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(4218..4640)
                     /gene="fabZ"
                     /locus_tag="VW411_000903"
     CDS             complement(4218..4640)
                     /gene="fabZ"
                     /locus_tag="VW411_000903"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEU9793660.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVVIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGTLTFAIGN"
     gene            complement(4637..5122)
                     /locus_tag="VW411_000904"
     CDS             complement(4637..5122)
                     /locus_tag="VW411_000904"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEU9793661.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(5125..6360)
                     /gene="fabF"
                     /locus_tag="VW411_000905"
     CDS             complement(5125..6360)
                     /gene="fabF"
                     /locus_tag="VW411_000905"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU9793662.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(6382..7113)
                     /gene="fabG"
                     /locus_tag="VW411_000906"
     CDS             complement(6382..7113)
                     /gene="fabG"
                     /locus_tag="VW411_000906"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEU9793663.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVVAIDGGLSM"
     gene            complement(7147..8067)
                     /gene="fabD"
                     /locus_tag="VW411_000907"
     CDS             complement(7147..8067)
                     /gene="fabD"
                     /locus_tag="VW411_000907"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU9793664.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            complement(8060..9034)
                     /gene="fabK"
                     /locus_tag="VW411_000908"
     CDS             complement(8060..9034)
                     /gene="fabK"
                     /locus_tag="VW411_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEU9793665.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVD
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWTGVVRND"
     gene            complement(9153..9377)
                     /locus_tag="VW411_000909"
     CDS             complement(9153..9377)
                     /locus_tag="VW411_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU9793666.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(9437..10411)
                     /locus_tag="VW411_000910"
     CDS             complement(9437..10411)
                     /locus_tag="VW411_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEU9793667.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(10411..10845)
                     /locus_tag="VW411_000911"
     CDS             complement(10411..10845)
                     /locus_tag="VW411_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9793668.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(10939..11724)
                     /locus_tag="VW411_000912"
     CDS             complement(10939..11724)
                     /locus_tag="VW411_000912"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEU9793669.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            12049..13413
                     /locus_tag="VW411_000913"
     CDS             12049..13413
                     /locus_tag="VW411_000913"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEU9793670.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKNQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            13416..13787
                     /locus_tag="VW411_000914"
     CDS             13416..13787
                     /locus_tag="VW411_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEU9793671.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            14000..15274
                     /gene="serS"
                     /locus_tag="VW411_000915"
     CDS             14000..15274
                     /gene="serS"
                     /locus_tag="VW411_000915"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEU9793672.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(15299..16198)
                     /locus_tag="VW411_000916"
     CDS             complement(15299..16198)
                     /locus_tag="VW411_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEU9793673.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLIGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(16308..16856)
                     /locus_tag="VW411_000917"
     CDS             complement(16308..16856)
                     /locus_tag="VW411_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU9793674.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(17074..18396)
                     /locus_tag="VW411_000918"
     CDS             complement(17074..18396)
                     /locus_tag="VW411_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEU9793675.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTIRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSIAESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMAIIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTNFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGVRFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(18487..18575)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(18739..21075)
                     /locus_tag="VW411_000919"
     CDS             complement(18739..21075)
                     /locus_tag="VW411_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEU9793676.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QNFYTNLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQLLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(21179..21727)
                     /locus_tag="VW411_000920"
     CDS             complement(21179..21727)
                     /locus_tag="VW411_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEU9793677.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPVTTSWLKQIWVTNLIG"
     gene            complement(21724..22026)
                     /gene="zapA"
                     /locus_tag="VW411_000921"
     CDS             complement(21724..22026)
                     /gene="zapA"
                     /locus_tag="VW411_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEU9793678.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            22113..22994
                     /locus_tag="VW411_000922"
     CDS             22113..22994
                     /locus_tag="VW411_000922"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEU9793679.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHDFLRKLGVGDSKTLTDQKIRQITPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLSVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            22998..23612
                     /gene="lepB"
                     /locus_tag="VW411_000923"
     CDS             22998..23612
                     /gene="lepB"
                     /locus_tag="VW411_000923"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU9793680.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRLWPITRIGTF"
     gene            23686..23814
                     /locus_tag="VW411_000924"
                     /pseudo
     CDS             23686..23814
                     /locus_tag="VW411_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chlorohydrolase"
     gene            23891..26257
                     /locus_tag="VW411_000925"
     CDS             23891..26257
                     /locus_tag="VW411_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEU9793681.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMILAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLETIQKHITTVEQELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDFRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSKESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKRDRVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDRIPAMIGITDTDLKEIFGK"
     gene            complement(26305..27588)
                     /locus_tag="VW411_000926"
     CDS             complement(26305..27588)
                     /locus_tag="VW411_000926"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEU9793682.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDQKFGEEALYQDAMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVITAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYSKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
     gene            complement(28374..29510)
                     /locus_tag="VW411_000927"
     CDS             complement(28374..29510)
                     /locus_tag="VW411_000927"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEU9793683.1"
                     /translation="MKHSVHFGAGNIGRGFIGEILFKNGFHIDFVDVNNQIIHALNEK
                     GKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQKTDIITTAIGPNILPFIAELL
                     AKGIEARRVAGNTQALDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAV
                     DRIVPAQSHEDSLFVVVEPFNEWVVETKRLKNPDLRLKDVHYEEDLEPFIERKLFSVN
                     SGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLIAKWNFDKKELENYHK
                     VIIERLENPFIVDEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYVFD
                     YRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDQELIEQIVEYI"
     gene            complement(29572..30009)
                     /locus_tag="VW411_000928"
     CDS             complement(29572..30009)
                     /locus_tag="VW411_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9793684.1"
                     /translation="MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIE
                     RDKELSVYMGNFIAIPHGTDAAKNDVLKSGITVVQVPRGVDFGNVSNPQVATVLFGIA
                     GIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE"
     gene            complement(30011..31966)
                     /locus_tag="VW411_000929"
     CDS             complement(30011..31966)
                     /locus_tag="VW411_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9793685.1"
                     /translation="MLLTKREEQLLKAFLHVGKLSIQDMTEILQVSSRTIYRTLSDLT
                     DSMEQYGIEITKHGKYYILTGELDDLPTELEVLVEYSPQERQELITYRLLTESGFVTN
                     EALQECTKVSNVTIIQDISDIDKRLLDFDLKIERQKGYRISGDSVGKRRFLAILLTNC
                     ISVADFSTGNFGSFDILEADRTGLASQIVNKQLSGFPDMDARMKMFFAILLSLIGQEQ
                     NIENSPNTSKQALEISQKIFQAYSKQTAQFYSIQEIIYFASILDELIIKRQDNPLFTE
                     KFDGEFFYNISNLIDTVSMYTKIDFFKDKVLFNFLFHHIRLSLGVPILFQGENLPESI
                     QILVERNKFLYTVISLLVNDIFPKYLHTEYEYGMIALHFISSLGRSPEIYPVRVLLLT
                     DERRVTRDLLVSKIKSVAPFVELIDIQSLVDYHSIDLSQYDYILSTKPLTNQEIDVIS
                     SFPTVKELLELQERLQYVQAHRTIVARDAIAPEKSYDLQDYLISSSQLLSQFELVQLE
                     NNQSFEHTVEQIIQYQKNVSDRAYLTRKLLSHFQNSPMAIPNTGLVLLHSQSSKVTTN
                     SFTMFELKLPISALSMKREEEEVKRCLLMLMSKEASEEARDLMTAISQSIIENHLYTE
                     IYKTGNQSIIYQMLNTIFNEKIKKLEN"
     gene            complement(31990..33759)
                     /locus_tag="VW411_000930"
     CDS             complement(31990..33759)
                     /locus_tag="VW411_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEU9793686.1"
                     /translation="MEEKVSLKVRVQKLGTSLSNMVMPNIGAFIAWGVLTALFIADGY
                     LPNEQLATVVGPMLTYLLPILIGYTGGYMIHGQRGAVVGAIATVGAITGSSVPMFIGA
                     MVMGPLGGWTIKKFDEKFQEKIRPGFEMLVNNFSAGLVGFALLLLAFYAIGPVVSTLT
                     GAVGNGVEAIVNARLLPMANIIIEPAKVLFLNNALNHGIFTPLGVEQVAQAGKSILFL
                     LEANPGPGLGILLAYAVFGKGSAKSSSWGAMVIHFFGGIHEIYFPYVMMKPTLFLAAM
                     AGGISGTFTFQLLDAGLKSPASPGSIIAIIATAPKGVWPHLNVLLGVLVAAVVSFLVA
                     ALILHADKSTEDSLEAAQAATQAAKAQSKGQLVSTSVDAVVSTDSVEKIIFACDAGMG
                     SSAMGASILRDKVKKAGLEIPVSNQAISNLLDTPKTLIVTQEELTPRAKDKSPSAIHV
                     SVDNFLASSRYDEIVASLTGASPIAEIEGDIPTSAPVDSQESDLNHIDAVVVAYGKAQ
                     GTATMGCETIRAIFRNKNIRIPVSTAKISELGEFNSKNIMIVTTISLQAEVQQAAPNS
                     QFLIVDSLVTTPEYDKMAARMYK"
BASE COUNT        10081 a         7608 c         6406 g         9824 t
ORIGIN      
        1 ggatttaccc actacaaata ttatagagcc aacaataaaa agaaaaagtg tttgatagat
       61 atcaaacact tttttctttg cctcccacta tctaaaaaaa tgataataga tataattgta
      121 aacaaaaatc cagataggtt ttgcatgatt gagaaagtta aaaaaactat ggcagagaat
      181 cgttaatctc agattgtcgg tagaacgata aacaagggca aaaaagaaac caatcagact
      241 ataatataat aaactaattg gatctctgtg agatagtatc aaatggctaa tcccaaagat
      301 gatagcagat aggataacat ccaaatagta cttggactag ggaaagaagg tattcataaa
      361 ataccctcta tcaagagtct cctcaaaaac aggaccgatg attacaggca ggacaaaaga
      421 taagatagtc gataaaaagg ttggttgtcc atttgaaaaa agcacggtaa aatactcatc
      481 atgaatattc ctatgattaa tcaaatgagc atagcgtgcc caaaaattac cgagaatctg
      541 ataaaccaca taagttgcaa ataagtagaa gacaaatgac cagttccagc tctttttctc
      601 aaagataaag agcatctttt tcttttttaa cctccaaatt aatagaagga aacttcccac
      661 taatcccatt gttaaaataa gagaatagac atcagctcct aaccctaaaa tgatcgtcac
      721 atacaatcca attgtttgtg gtaaataggt agatagtaaa ataataagca aaaatattcc
      781 aaattgtctt agtttttttg tgtttctcat cgtacttttt tgaaagatta ccctgctcgg
      841 aagccgtact tccaagcatc tatataagaa ttaagtgccc cttgcctcat atagggagca
      901 aattctctat aatataacca tctactatat ccatcttccc aaacagcaag accacctgaa
      961 gtttgctcca agtcctcagt tgaaagaact gtaaatgtat ttgtacctgt cattgcaagt
     1021 accttcttaa aatagattgt tgtaggctca catttatagt atatttcttt ttttgtctat
     1081 tttatagccc atctcctcaa ctggcaattt ttcgacctga attacatttt tccataaaaa
     1141 atgagacctt tctagtctca tttagtcatt cttagtattt tctaaatcgt tgatagcgtt
     1201 cttccagcaa ctcttctagc ggtttttgtg aaagtctagc cagctccgtt tggagttctt
     1261 ttttgacact cttaatcagt tctttactag aaagtcctat ttcagaaatc accttatcca
     1321 ccacgtccat ttctaacagt tcatgcgaag tgattttcat cagttctgct gcttccatag
     1381 cgcgagtacc gtccttccat aaaatggaag caaagccttc tggactgaga atggcataga
     1441 tagaattttc cagcatccag acacggtccg cgacagctag agccagagcc ccgcctgaac
     1501 caccttcacc gataataatg gcgataatag gaactttcag gtcactcatt tccatgagat
     1561 tgcgagcgat agcttcccct tgaccacgtt cttccgctcc gacaccagga taagcacctg
     1621 ctgtattgat aaaggtcaca actggacggc caaatttctc agcctgtttc atcaaccgca
     1681 gtgcctttcg gtagccttct ggatgtggtt ggccaaaatt ccgtttgagg ttgtcttgca
     1741 aactcttgcc tttttggata ccaaccactg ttacagcttg gtctccaagc caaccaatac
     1801 caccaacaac tgcaccatca tcacgaaaag aacggtcacc atgtaattgg ataaattcat
     1861 caaaaatgcc tgtcgcaaag tccaaggttg tcaagcgact ctgctcacgc gcttctctga
     1921 ctatttttgc aatattcatc taggactccc tccatgcaat ctgactaggc tagcaatcgt
     1981 atctggtaag tctcttcttt tgacaatagc atccacaaag ccatgttcta ataggaattc
     2041 tgccttttgg aaatcctcag gcaagctttc acgaaccgta ttttcaatca cacgacgccc
     2101 agcaaaacca accaagctct gtggttcagc cagaatgata tcgccttcca tagcgaaaga
     2161 agctgtcaca ccaccagtcg ttggatctgt caaaatggtc aggtaaaaga gaccagcatt
     2221 tgaatggcgt ttaaccgccg cagagatctt agccatctgc atgagactca tgattccttc
     2281 ctgcatacgg gctccaccag aggctgtgaa taggacaact ggcaattttt cgacagtcgc
     2341 atactcaaac aaacgagtga ttttttcacc tacaaccgta cccatagaag ccatgataaa
     2401 gttagaatcc ataatcccaa gagccacagt ctgaccttta ataagagcag ttcctgtcac
     2461 aacggcttca tgcagacctg ttttttcacg catagatgcc agtttctttt ggtaaccagg
     2521 gaaatgcaag ggatccttgc tttcaatccc tgtaaacaat tctttgaagg ttcccatatc
     2581 aatcgtcaaa gccaagcgtt cttgggcaga aatacgaaag gtatagctac agtgcggaca
     2641 gatacgttca cttcccagat ccttctgata gatggtatgc ttacagcctg gacactggga
     2701 aaataattca tctggaacct ctggcttagc ttgaggtttt tccctaaccg aacgattggg
     2761 attgattcga atatacttat cttttttact aaatagagcc attgattccc cttttcggtt
     2821 taaactctta aagtcatttt attctttttc ttgatattta ggtaagaagg tttccatcaa
     2881 gaaggaagta tcataatccc cagcaatgac attgcgatct gaaatgaggt caagctggaa
     2941 atctgcattg gtctgcactc cttcaatttc taattcatag agggcacgtt gcattttcat
     3001 caaggcgtca aaacgatttt cgccgtgtac tatgattttg gcaatcatac tatcataata
     3061 aggcggaatg gtataacctg gataaactgc tgaatccacg cgcaagccaa ctccaccact
     3121 tggcagatag agattagtaa tcttacctgg acttggagca aagttaaagg ctgggttttc
     3181 tgcattgata cgacactcga tggcatgacc gcgtaggaca atatcttctt gcttaacaga
     3241 caaaggctga cctgccgcaa tgcaaatctg ttccttaacg atatcaacac ctgaaacaaa
     3301 ctctgttact ggatgttcta cctgaacacg agtattcatc tccatgaaat agaaattgct
     3361 acttgcttca tcaagaagaa attcaatggt tcctgcattc tcatagccaa caaactctgc
     3421 cgctcgaaca gcagcagcac ctatttcatg acgcagcgtt tttccgattg caatcgaggg
     3481 actttcttcc aaaacctttt ggttattcct ttgaagagaa caatcccgtt cacccaagtg
     3541 aatcacatgt ccatgctcat cacctaggat ttgaacctca atgtgccgag ctggatagat
     3601 aacccgttct atgtacatgg caccattgcc ataattggcc ttggcctcac tagaggcagt
     3661 ttcaaaggca gaaacgaggt catctggttt ttcaacctta cgaatccctt taccacctcc
     3721 acctgctgaa gccttgagca taacaggata gccaattttt tcagcaacaa tcaaagcttc
     3781 ttcagagtta tgcacttctc catctgaacc tggtataaca ggcacacctg ctttaatcat
     3841 ctgagcacgc gcattgatct tatcccccat catatccata acatgaccag atggaccgat
     3901 aaacttgata cctacttctt cacacatggt cgcaaatttg gaattttcac tgagaaatcc
     3961 aaaaccaggg tgaatagctt ctgcctcagt caagactgca gctgatagaa ctgcattaat
     4021 attgagataa gactctgttg ccttgccagg accaatacaa actgcttcat ctgccaaaag
     4081 cgtatgaaga gcttccttat cagcagttga ataaaccgct accgtcgcaa tccccaattc
     4141 acgtgccgca cggataatac gaaccgcaat ttcaccacga ttggcaatta aaatttttcg
     4201 aaacatggag aacctcctta gttcccaatt gcaaaagtaa gggtaccact ggctgcaagc
     4261 ttgccatcca cttcagcctt tgcttcaacc acagctatgg tgccacgacg ttttacaaaa
     4321 gtcgctgtca taaccaattg gtcgcctggt acaacttgct tcttgaactt aaccttgtcc
     4381 ataccagcgt aaaagaccag ttttccttta ttttcaggtt ttgataactc caacacaccg
     4441 gcagtttgcg ccaaggcttc cataatcaca acacctggca taactgggta ttgaggaaag
     4501 tggccgttaa agaaaggctc gttgatggtc acatttttga tagcaacaat ggtatcctcg
     4561 ctcacttcca agacacggtc cactagaagc ataggataac ggtggggaag agcttctttg
     4621 attccttgaa tatcgatcat ttgatacgta ccaatccttt accaaactca accatttctt
     4681 cgttagagac gagaatttcc gttaccacac catccttagg agctgggatt tcattcatga
     4741 ctttcatggc ttcgataatt accaatgttt gacctttttt gacactatca ccaactgtaa
     4801 cgaaggcagg tttatctggt ccagcagcca agtaaaccac tccaacaagt ggactctcta
     4861 caagatttcc ctcagtagcc acacttgctt cagctggagc tggaacttct tctgctacag
     4921 tctctgctgg agcagatgta ggagctactg gactcggtgt tgctagaacg ggtgctggag
     4981 cgacttgagt tgcaacttca ggcacaggtc ttgcttcatt cttgctaaac tgcaactcat
     5041 ccgtcccatt tttataagaa aattctctca aacttgactg gtcaaattga gtcatcaagt
     5101 ctttaatatc gtttaaattc atacttatct attctcccaa cgtttgaaag caagaactgc
     5161 attgtggcct ccaaaaccaa aagtatttga aatagcgtat ggaatttctt tctccaagcc
     5221 ttgtccataa acgacattag cttcgatata atctgatact tcacttgtcc cagctgtcat
     5281 tggtacaaag ttatgacgca tagcttcgat ggtgacgata gcttctactg cacccgcagc
     5341 ccccagcaaa tgtcctgtaa aagacttggt tgatgataca ggtacttcct taccaagaac
     5401 agctacgata gcaccacttt ctcctttttc attggcagga gttgacgttc cgtgagcatt
     5461 gacataggct acttgctctg gagaaatctc agcttcttcc aaggctagtt tgatggcctt
     5521 gatagctccc tgaccttctg gatgtggaga agtcatgtgg taggcatcac aagtatttcc
     5581 gtaaccaacc acttcagcca ggatagtagc tccacgtttt tcagcgtgtt caagactttc
     5641 tagaaccaac atccctgaac cttcacccat aacaaaccca ttgcgatcct tatcaaatgg
     5701 gatcgaagca cgagttggat cctctgtagt agagagagct gttaaggctt ggaaaccagc
     5761 gatggcaaaa ggtgtgatag aagcttctgt tcctcccacc aacatcacat cttggaaacc
     5821 aaacttaatg gagcggaagg catccccaat cgcatcattt gatgaagagc aggcagtatt
     5881 gatagattta caaacaccgt ttgcaccaaa acgcatggct acattcccag aagccatatt
     5941 tggtaaagct tttggaagag tcattggttt gacacgtttg ggtccttttt catgaaggcg
     6001 aagtacctga tcttcaattt ccttgattcc accaatacca gatgcaacga taacaccaaa
     6061 acgatcccta ttaagagcct ctacatcaag attggcatga tttacagcct cttgggctgc
     6121 atacaaggca tataaagaat agttatcaaa acggttggta tcttttttta caaagtattt
     6181 atcgaacgga aaatcttgga tttctgccgc attatgcaca tcaaagtcac tatgatcaaa
     6241 ttttgtaatg ccaccaatgc cgattttccc agttgctaaa ctattccaaa attcttctgg
     6301 tgtatttccg attggagatg ttactccata acctgttacc actactcgat ttagtttcat
     6361 tcttttcacc tctagctttc gctacatact taagccacca tcaatggcaa ccacttgtcc
     6421 agttagataa tcttggcctg ctaaaaatac tgtcaaatct gcaacctgct ctgcctgccc
     6481 aaattctttc atcggaatct gagctagtgt agcttcctta atcttatctg acaggatagc
     6541 ggtcatatca gactcaatca ttcctggagc aatcacattg actcgtatat tccgactagc
     6601 gacctcgcgt gccacagact tggtaaagcc aatcaagcca gccttagaag cagcataatt
     6661 agcttgacca atattcccca tcaaaccaac aacactagac atattaatga tagcaccttc
     6721 tctggctttc atcatcggtt tcaagactga ttgtgtcata ttaaaggcac cagtcagatt
     6781 gaccttgagc actttttcaa aatctgcttc tgtcatcttg agcataagag tatcttgggt
     6841 aatccctgca ttgttgacca aaacatctac tgaacccagt tctgcaatag cttgatcaat
     6901 catacgctta gcgtctgcaa aatctgatac atctcctgaa atgggaacca ccttgatacc
     6961 atagtttgaa aactcagcga gcaattcttc tgagattgcc ccacgactgt ttaagacaat
     7021 gttggctcct gcttgagcaa acttgtgggc gatggcaaga ccaattccac gactcgaacc
     7081 tgtaataaag atatttttat gttctagttt catttttttc ctttcaaaac ttctacttat
     7141 tttagtctat ttttctaaaa gtgctactaa actcgcttga tcttccacat gagctaagtg
     7201 agcagtttga tcaatttttt taacaaaacc tgacaagact ttccccggtc caatctcgat
     7261 aaagttgctt atgcctgctt cttgcatgac cccaatactt tcatagaaac gaacgggttc
     7321 cttgacctga cgcgtcaaga gctgagcaat gtcctctttt tgcatcacag cagcttctgt
     7381 attgccgact aggggacaag taaaatctga aaaacttacc tgagctagag tttcagctag
     7441 tttctggcta gcaggttcaa ggagagcggt gtgaaaggga cctgacacct taagaggaat
     7501 caagcgtttg gcacctgctt cttgcaaaag ttcaaccgct cgatcaactg caaccacttc
     7561 tccagcaatg acgatttgtg caggtgtgtt atagttggct ggagtaacca ctccaagttc
     7621 agaagctttt tgacaggctt cttcaatgac ctctactggc gtattgagaa ctgctaccat
     7681 cttgccagag tcagcaggag ccgcttcttc catataggct ccacgcttag ctaccaaggc
     7741 aaccgcatct tcaaaatcca aggcgccact tgccaccaag gcagagtatt ctccaagaga
     7801 caaaccagca accatatcag gctgatagcc cttttcttgc aataaacggt agatagcaac
     7861 cgaagtcgct agaatggctg gttgcgtata gcgggtctga ttgagtttgt cttcttccgt
     7921 atcgatgaga taacgcaaat cataaccgag cacctggctc gctcgatcaa tcgtttcttt
     7981 aacaatcgga tactgatcat agaaatcccg tcccatccct agatactggg caccttgacc
     8041 agcaaataaa aaggctgttt tagtcatttc ttacaactcc tgtccagcga gaggcttctt
     8101 cttgaatttt cttagcggct ccgtaataca aatcttttag gatttcttca gctgtttctt
     8161 ctttagaaac aagccctgcg atttgacctg ccataacaga gccaccatcc acatcaccgt
     8221 gaacaactgc tttggctaga gcacctgctc ccatttgttc aaagatttct aaatcaggat
     8281 cttcttgctt aaaggcatct ttttcagcca gttcaaaatc tctagtcaac tgatttttaa
     8341 tagcacgaac agcatgacca aagtgctgag ctgaaatcgt agtatcaata tcccttgctt
     8401 ttaaaatttt ctccttgtag tttggatggg cattcgactc ttttgcaact acaaaccgtg
     8461 tccccacctg tacagcctct gcacctagca taaagccagc cgcagcacct tcaccatccg
     8521 caattcctcc tgcagcaata acaggaatag atatagctgt ggctacctgt cgcaccaagg
     8581 tcatggttgt taatttaccg atatgccccc cagcttccat tccttctgca ataacagcgt
     8641 ctgcaccgat tttttccatg cgtttagcta aagcgacact aggaacaaca ggaataacga
     8701 ttatcccagc ttcatggaaa cgttccatat acttgcttgg atttcctgct cctgttgtga
     8761 caactttaac accttcttca ataacgagat ccacgatgtc ttccacaaag ggagataaga
     8821 gcatgatgtt gaccccaaag ggtttatcag tcaatgattt gattttatca atattggcct
     8881 tgacaacttc tttcggggca tttcccccac cgataattcc taatcctcca gccttggaaa
     8941 cagcccctgc caaatcacca tcagcaaccc aggccatccc tccttggaaa ataggataat
     9001 caatcttcaa taattctgta atacgcgttt tcatagtgcc tccaaccttc cttgcttacg
     9061 taatagttcg atttcaccat aatttgacag tcaaactatt acctaaacaa gagggagtgg
     9121 gtttctccct actccttcta ctaatattct gcttattttg cttgctcttc aacgtaagca
     9181 accaagtcac caactgtttt caagtcattt tctgcttcga tttggatatc aaaagcatct
     9241 tcgatttctg agattacttg gaacaagtcc aatgaatctg cgtccaaatc atcaaaagtt
     9301 gattcaagtg ttacttctga tgcgtctttt ccaagttctt caacgataat ttcttgtact
     9361 ttttcaaata ctgccatgat aggactcctt taaaataaat agttttttta taacaatgtg
     9421 ttcaccacat gattacctaa attgtaagaa tgagcgtgcc ccaggtcaag cctccaccga
     9481 agcctgatag aagaacagtc tggctaccat ctaaagggat gagaccttgt tctacacact
     9541 ctgaaagtaa aatcgggata ctggctgcac tggtattgcc atattccatc atattggctg
     9601 gaagtttggc tcggtcaaca ccaatttttc tagccatctt atccaaaata cggtcattgg
     9661 cttgatgaag tagcagataa tccaagtctg tcacctctat aggagattca tcaatagtct
     9721 gcttgataga cttggctaca tctcgaatgg caaaatcaaa gactgtgcgt ccatccatct
     9781 tcaaaaacga atctgcactt tcttgatctg aaaatggaga atgtaaacct gaatgcccat
     9841 aagttaaaca ctcgctgcga cttccatcgc tattgagact ctcagctaag aaatgctctt
     9901 gctcgctagc ttctaacaag acaccaccag caccatctcc aaacaacaca gctgttgatc
     9961 gatccgacca atcgactgcc ttagagaggg tttcactacc aatcaccaag cctttttgaa
    10021 agcgaccaga agcgataaac ttttcagcag ttgaaagagc aaatacaaat ccactgcaag
    10081 ccgcggttaa gtcaaaagca aaggctttat tagcaccaat attagcttga acacgagcag
    10141 ctgtagaggg catcatcgaa tctggagtaa tggtagctag gatgataaaa tccagttctt
    10201 ctcctgttat tccagctttt gccatcagtt tcttagcaac ctctgtagcc aaatcactgg
    10261 tagattctgt tcttgaaata tgcctttgtc gtattcccgt tcgacttgaa atccactcat
    10321 cattggtatc cataatctga gccaagtcgt gatttgtaac cacttgctct ggcacataat
    10381 gagcaacctg acttattttt gcaaaagcca ttatttcaaa tcctccaaaa attggtaaag
    10441 attagtcaaa cctttaccca tgacagcaat ttcttcctcg ctcatgccat caataatttt
    10501 ttctaccatg gccttgtgga agcgtttatg cagtctatga atcaagcgac ccttctttgt
    10561 caaatgcaga tgcaccacac gacgatcctg ttctgaccga actcgctcaa tgtagccctt
    10621 acgctctaaa ttgttcaaac ttgtcgtaac agttccaaga gttaccatca actcttttga
    10681 cacttgactt ggagtcacgt ctggagcctt accgatgaca tcaatcgtat gcatttcttt
    10741 gatggagata tccttaaaac gactacctct caagttcact tcctcaatta caaggacatt
    10801 gttaaatata gatgttaaat attcattgat tcgttggtag tccatttttc atatccctcc
    10861 ttcttcaaaa aacttttaca atcaaaacat ttgacagaaa gaattatatc aaaattcaaa
    10921 gaatgatgca agtatttttt attttcctgt aaatttaggt cttcttctct ccgaatgagc
    10981 tcgcactcct tctttgaaat cctctgtttg agctaaggat ttctgtaggt tcagttctaa
    11041 agtagcgtaa ccctgccagt ctttaaattg gctctcccaa accaacttct taatggcagc
    11101 ataggaatta cttgaagcac gtcttaattt tttaagaagc tgttctctcg tcttttcaag
    11161 tttttcagct tcagaaacgc ggtaaaccaa cccccattct agggcttttt ctgccgttaa
    11221 agcctctcct gtcatagcta attgagcagc acgcgtcaca ccgatactgc ggctcaagag
    11281 atgaatcccc cctgcatctg gagccaaacc aacaccaaca aaggcttgga taaacttagc
    11341 cttatccgtc gccaaacaga aatctgccgc aacagccata ttcgctgcgg cacctgcaac
    11401 agcaccgtca acttccatta aaacaggttt ggctatttgc ttgattttat aagaaatcgt
    11461 attgaccaac tctgcgattt ttgtcaatga tggaatatca tcctcatcca ctgcccgctt
    11521 catctctacc aaatctcccc caactgagaa gacctttcca ttggcattga ttaagataaa
    11581 atgcacagct ggattttctt ctgccaaagt cagagcttct aaaatctcct cacacatggg
    11641 aatatgaaaa ccatttgcga cctcaggacg gttcaaggta aggattgcca agtcctcttc
    11701 aagttgataa ataatgtgtt ccatcagggc tcctttttat tttataagca gtttatactc
    11761 ttcgaaaatc tctttaaacc gcgtcagctt ccatctgcaa cctcaaaaca gtattttgag
    11821 ctgacttcgt ctagcttctt agtttgctct ttgattttca ttgagtgtta aattatttca
    11881 ctgtcaaagt atatcttttt taggagcaga aagcaagaaa aaatcgactg aaagtgtctc
    11941 agccgataag aaaggctcgc tttaggctag aaagcatctt attttcttca ttgaaaaaag
    12001 cctttctttc tgctataatc gtataaaata cttactttag gagttcttat gaaagttgtt
    12061 aaatttggag gtagttctct tgcctctgct agtcaattag aaaaagtttt aaacatcgtc
    12121 aaaagcgatt cagagcgtcg ttttgtagtc gtttctgcgc ctggtaaacg caatgctgaa
    12181 gatactaagg ttacggatgc cctgattaaa tactaccgcg actatgttgc gggtaacgat
    12241 attagcaaga accaaagctg gattatcgac cgctatgctg ctatggttag tgaattggga
    12301 ctaaaaccag ctgtgctaga aaaaatttct aaaagcattc acgccttggc cactcttcct
    12361 attgaagaaa atgaatttct ctacgatact ttcctagcag ccggtgaaaa taacaatgcc
    12421 aaattgattg ctgcctactt taaccaaaat ggtatcgatg cacgctatat gcaccctaga
    12481 gaagctggga ttgtggtcac aagtgaacct ggtcacgctc gcatcattcc atcaagttat
    12541 gacaagattg aagaattgac aaacaccaat gaagtccttg tcattcctgg tttctttggt
    12601 gtcactaagg aaaatcaaat ctgtactttc tcacgtggag gttctgatat tacaggttct
    12661 atcattgctg ctggtgtcaa agctgacctc tatgaaaact ttacggacgt tgatggtatc
    12721 tttgcagccc accctggtat tatccaccaa ccacactcga ttcctgagtt gacctaccgt
    12781 gaaatgcgcg agttggccta tgcaggcttc tcagtccttc atgacgaggc tcttcttcct
    12841 gcctaccgtg gaaaaattcc tctggttatc aagaatacca acaaccctga ccatccaggt
    12901 actcgtatcg ttctaaaaca cagtaatgat gaatttccag ttgtgggaat tgctggtgac
    12961 tcaggctttg tcagcattaa catgtcgaaa tacctcatga accgtgaggt tggatttggc
    13021 cgcaaggttc tgcaaatcct ggaagaactt aacatcggtt gggaacatat gccaacaggt
    13081 atcgacgatc tttctatcat tctccgttct cgccaactaa ctcccatcaa ggaagaagaa
    13141 atcctgcgtc agttggttca aaaggctaaa gtagatcatg cagaaatcga acacgacctt
    13201 tctatcatta tgattgttgg tgaaaaaatg aagagccata tcggagtgac tgctactgcg
    13261 acacgcgctc tatctgaaaa caaaatcaac atccagatga tgtctcaagg ttctagtgaa
    13321 gtttctatta tgtttgttgt caataaagac caagagaaag cagcaattaa agccctctac
    13381 aatgcctttt ttggagaaag taaggaagac taagcatggc ccaatcactt aacaaaacag
    13441 tactcctcag tacgacaggc acttcctacc tctctatcgc tgggaaagtt gggaaattcc
    13501 ttgtcggaga tcaggctttg gaattttacc cagatgtcaa tgtcgaacaa tttatccaga
    13561 ttccttggag ccatatcaat cagattggag ccaatgtcac tggacgcaaa atcagccgcc
    13621 acttcgaagt ctttacagac aaaggaaaat tcctctttgc ttcaaaagac tcaggtgcca
    13681 ttctcaaaat tgcacgcgaa aaactaggca atgacaaggt cgtcaaactt ccgaccctga
    13741 ttcagacaat tagccaaaaa tttaaaaatc tatttgcaaa aaagtagaaa ctttagtata
    13801 atcatagcaa cggataagta ggttatcttc ttctttcaga aagtctgcgg gtactgcgag
    13861 cagataggag ggataggtga aattctaccg ttagtgacaa ttacatacac aatcaagtgc
    13921 acacctgtga agttggatgg aaccgtggcc ctgccactcc aacgttttgt cagggtgctt
    13981 ttttcataaa ggagttctta tgttagatat caaacgtatt cgtacagatt ttgaagctgt
    14041 cgcagaaaaa ttagctacac gtggtgtaga tgctgctgtc ttgaatgaaa tgaaagaaat
    14101 cgatgctaaa cgtcgtaaca tcttggtcaa ggttgaaact ctcaaagcag aacgtaacac
    14161 agtttctgct gagattgccc aagctaagcg caacaaggaa aatacagatg acaagattgc
    14221 tgccatgcaa aatctatctg ctgaggttaa agccttggat gctgaattgg cagaaatcga
    14281 tgctaaattg acagaattta caacgactct tccaaatatc ccagctgaca gcgttcctgt
    14341 tggggctgac gaagacgaca atgtggaagt tcgccgttgg ggtactccac gcgagtttga
    14401 cttcgaacct aaagctcact gggatctcgg tgaagacctt ggtatccttg actgggaacg
    14461 cggtggtaag gtaacaggcg ctcgcttcct cttctataaa ggcctcggtg ctcgtttgga
    14521 acgtgctatc tacaacttta tgttggatga acatggaaaa gaaggctata ctgaagtcat
    14581 cacaccttac atagtcaacc atgattctat gtttggtact ggtcagtatc caaaatttaa
    14641 ggaagatact tttgaactca gcgataccaa ctttgtcttg attccaactg ctgaagttcc
    14701 tctgacaaac tactaccgtg atgaaatctt agacggcaaa gatcttccaa tctacttcac
    14761 tgccatgagt ccgtcattcc gttctgaggc tggttctgcc ggtcgtgata cgcgtggctt
    14821 gatccgtttg caccaattcc acaaggttga aatggtcaaa tttgccaaac cagaagaatc
    14881 ttacgaagaa ttggaaaaaa tgacagccaa cgctgaaaac attcttcaaa aactcaacct
    14941 tccataccgt gtcgttgctc tctctactgg agatatgggc ttctcagctg cgaagactta
    15001 cgacttggaa gtgtggattc cagcacaaaa caattaccgt gaaatctcaa gctgttcaaa
    15061 cacagaagat ttccaagccc gtcgtgccca aatccgttac cgtgatgaag cagatggcaa
    15121 ggtgaaactc cttcatacct tgaacggttc tggacttgca gttggacgta cagtggctgc
    15181 aattcttgaa aattaccaaa atgaagatgg ttctgtgacc atcccagaag cacttcgtcc
    15241 atacatgggt ggagctgaag tcatcaaacc ataaaaaata aggtttagct atttctagct
    15301 agaccttttt tcgtaaccaa atcagataag cacctagtac aaagaataaa atagttaggc
    15361 atataatggt ttcagccaat accaggtaat ccagaaatgg aagtttcaaa attccctgag
    15421 ccatcttgag cgaggtcgct gtgataatgg ttgggaaggt gagggctgag aaggctggtt
    15481 gaaaaccttg ttttaaaatg ttgggcagac gagttaaaac aaagaaaaag aaggattgag
    15541 aagccaaaat catgacaatc aagacccaag tcggcaggct ggttcctcct actcgaacta
    15601 gagaagccaa gagtagagag aaaggagcac agtagattcc ttcttgtcca agcaaggcta
    15661 gtgggagtgg atgtttcttt aaatcgctat aaataagggg atagagatag aaggtcaaga
    15721 gaaaaccaaa actcaaggtc gcataggcaa tttcgataat acctaccaga ggataggtca
    15781 aggcagccac tgctatcccc acatagagaa ccgtccagct tggagtggca tgaaccctcc
    15841 gccctggaca agcaaacttg atggtaaaac cagcaatcaa ggtcaaatcc aagagaaatg
    15901 aaaaccacca aatcccttgt gctaccaaag gaagataaga gaatacgcga aagacatagg
    15961 tcgataaaat catcccagcc ataggaaagg ttgccattcc tgacaaaaga gggggcttgg
    16021 tcaattcttg cttggtttct ttccaattaa agagatgcag aattagaaag taaatccata
    16081 aaaccaaacc aatcagacta aaaagatggg atagaaccgg caacgtatct aaaataagat
    16141 ttccagctcc tgccaaacct agcaaacaac ctgaaaatac taaggggagt tttttcatcc
    16201 taacctccaa taatcatgtt agtttcagta taacataaaa gcgcttaaat gaggatttaa
    16261 aaaaacgaag tccgcttatt tcagacttca ttttactcag atatgaatta ggcataaggt
    16321 tgcaattctg gattaattgg tgtattagct aagttgttgg catagttaca gaggattgct
    16381 aggctgacac caaaaaccac atccaaggca ttttgttgag tgtagccagc ttctaaaaac
    16441 tcagacaagg cttcatctcc tacacgaccc ttggtattga taactgccaa ggtaaactta
    16501 gctagggtat ccaatttagg atctgtttca attggagtac gattgcgaag agcttgaatc
    16561 aagtcatcat tcatctggat ttgtttgatg gaaaaggctg tgtgacctgc gacacagaag
    16621 gcacaaccat tggtcacggc tgccgtgatt tgcaccactt cacgctcaac gggtgtcagg
    16681 ctgttgcgac ggtggataga tgagacaatt tggtaggctt ctaaaacagt cggggcattg
    16741 gccaagagac cgattaggtt gggaatatag ccattgttgt ctttttctac tgtttcaaga
    16801 atttctttca cttctgctgg tgctgactct actgtatgga tagtaaatgt tgtcataaga
    16861 tacctctttt cttattattg acactaatat tattggaaaa tcttataaaa tcctgattcc
    16921 taagtttatc taagataaag ctttattctc tcataagatt ttcgttgtta tattagttta
    16981 tcacacttcc aatcacttgt ataatatata ttatatatca ggctgataaa aattatttat
    17041 aggcaaaaaa atcacacgag ctgtgtgatt ccattatttg tcaaaatact ttttagtttc
    17101 agcaataacg actggcgaca agaccaagag ggcaatcaag tttggcagag ccatcaaggc
    17161 gttaacgata tctgcgataa tccagaccat atccaactcg ataaatcctc ctaacaagac
    17221 catgagcaca aaaaccacac ggtagagcca gataaagcga accccaaaga ggaactcaaa
    17281 acagcgttct ccgtaatagt tccaacctag aatcgttgta aaggcaaaaa gtacaaggaa
    17341 gatggtcaag agagcaggcc caaagtgtga aaagtttgtt gagaaagctg actgagtcaa
    17401 ggcaacccca ttcaagtcac cgctccaaac tccagttacc aagatggtca aaccagttag
    17461 agtacaaatg atgagggtat caataaaggt tcctgtcatg gaaatcaaac cttgctctac
    17521 tggttcattt gtcttggcag ctgcagctgc aataggagca gaacccagac cagattcgtt
    17581 tgagaacaca ccacgcgcca caccattttg aatagccatc cgaacgctag caccagcaaa
    17641 tccacctacc gcagcaaggg gactaaaagc tgaggtaaag actaaagcga ttgtgccagg
    17701 gatttttccg atattaaaga aaataactgt aagagttcct aagatataaa tgatggccat
    17761 aaaaggaaca acagtagttg aaaccttaga aatagacttg agtccaccaa agactgcaat
    17821 cgctacaaag acagacaaga cgagagctgt gatggctggc gaaatcgtcg ttgtattttg
    17881 gatagattct gcaatcgagt tgacttgggt gaaggttccg attcccaaga gagcaaccaa
    17941 tactcctgct actgcaaaca aaacagcaag tggtcgccac ttttctccca tccctagaag
    18001 gatataatgc atgggacctc ccgctactgc accatggtcg tccttggtgc ggtatttgat
    18061 ggccaagagt ccttccgcat acttggtagc cattccaaag aaagccgcca tccacatcca
    18121 aaatagagct cctggtccac caaccttgat agccgtcgca actcctatga tatttcctgt
    18181 tccaacagtt gatgccaagg ctgtacacag agctgcaaaa ctggatacat caccatgtcc
    18241 cttatcctgg ataaaaataa gctgaaaggc cttgggtaga cgcaaaacct gcaagagtcc
    18301 tagccgaata gttaggtaaa tccctgttcc gaccaataaa atcaagaggg gcggtcccca
    18361 agcaaaagca tcgattgatt taagcaattc taacatttcc ttctcctatc gtttcaaccc
    18421 caaaagaaag agcacatgca agatacatgt actctggaat gcttagataa atgctaaaaa
    18481 gcggtctatc ctagctctgt ccttttacct gagagtttga gcagttgcct gccttgcccc
    18541 ttcggtgcct ttacggtctc tccagagttc cgtccattta cagtcatgga aaatcaaacg
    18601 attccccact tctattaaac ttcattcggt gttggtattt aattgattct aattttacaa
    18661 aaaatgttgg cttttgtcaa tgtgtttatt agtaaaaatt agttcaacag tttttacttt
    18721 ataaagtcca gaatactgct atcctttaaa agtgacaata gtcgcaccac tgcctccagc
    18781 attttgtggg gcatagccga aactcttgac atgtttgttt ctttgcaagt atttggtaac
    18841 tccttcacgg atgactcctg ttccgatacc atggatgata tcaacttgag ccatattgtt
    18901 aagcaaggct tggtcgatga aggtatctag ctcattcatg gcttcttcat agcgcttgcc
    18961 tcgaagatcc agtctagctt gaggtcctcg cccagaagtt cgtttcacaa cattgacctg
    19021 tttcttcttg actggttttt cttgctgggc ttgaacaaga tcaaactctt tctcttccaa
    19081 ggtcatctta atcaagccaa cttgggcttc ccagcgaccg tccttgagtt gactggtcaa
    19141 ggtaccacgc tgaccataac tgagaaccac gatatcatct cccacctttg gagctcgttt
    19201 tttcttggcc ttttgaagga ccttattttt agacaagtcc actttttcag gagccaattt
    19261 tttcaacttg gccttggctt caatgatttc gtggggcttg agttgggatt tactgtggag
    19321 atttttgaga atctggtcac tttcacttag ggccatatcc acaatctcag cagcctgttc
    19381 acgcgccttg ttaagctcgg tttccttttc acgattaagc tcgttgtaga gtttttttag
    19441 cgcacggttc atcttgagat tttcttgctc cacctcacgg atattgtcca aacgtttgcg
    19501 gctttccagc gtctgctctt ctaattgctc aatgatacga ttgacgtcat tgtcctgatc
    19561 gatctgctga ctggcatctc ctacgataac ttcagatagg cctagacgtt tggcaatttc
    19621 aaaggcatta cttcggccag gaacaccctg cataaagcga taggtcgggc gaagagttgc
    19681 agtatcaaac tccatactgg cattttgcac aaaggctgtc tcaataccgt aggccttgag
    19741 ttctggatag tgggtcgtcg ccatggtctt gatttgacgc aggcgaaggt cctccagaat
    19801 agccatggca agggctgctc cctcttgggg atcagtacca gcccccaact catccaaaag
    19861 taagagtgaa tgttggttga ccttgccaag aatatccacg atattggtca tatgactaga
    19921 gaaggtagac aagctctgct caatagactg ctcatctcca atatcagcaa agatttcttc
    19981 aaaaatacca acacgacttc ccttgtctgc taaaatcggc aatcctgact gggccatgac
    20041 ctgtgtcaag cccagagttt tgagcatgat ggtcttccca cctgtattgg gacctgtaat
    20101 gacaatagct gttaaatctt gaccaaaata gacatcattt gcgacggcat ttttgaccaa
    20161 aggatggcag acatggagca gttgaatctc ttgattttct gacagctgag gcacgactgc
    20221 ttgtctttct tggataaatc gaaccttggc acgaatcaag tccagatgac cgataatcca
    20281 agcgtcatta gcaatctcag ccgcatgagg gcggacacgc tcagaaattt cttggagaat
    20341 gcgaagcatt tcatagcgct catctgctcg cagactagca atttcttcgc tcagtttgac
    20401 tacctcacgg ggttcgatat agacggtgtt tccactagca gaaatatcat gaacgacacc
    20461 tgcaatctta ttgcggtagg tgtttttgac tggtaaaacc tgacggccat ttctgctagc
    20521 aacaattcct tccgtcaaca gctgcgcttt ttgcttgagc aagtcttgta aaacatcgcg
    20581 tacctgactc tcgctatcat gtatttttcg acggattcgc gccaattctt cactggcaaa
    20641 attttcaatg aaacccgcat cattaaaggc ctgaagattt ccttgtaatt gcggaaaatc
    20701 atgtaatttc tcaaaccaaa gggctaattc ttccaagctg acattttcca gattggtgta
    20761 aaaattttga agttctcggc tggcaagaag cacgcgtttc aagagtagga actcctcgat
    20821 attgagatcc gctcccatct ccaacctctt gcagactcct gcaatttcct tagttgagag
    20881 aatagtaaaa tgcggttgct cgacgaaaag agcctgcatt tccttcatct cagcaaaagc
    20941 ctgtttgatt ttatctgctt tggcagtcgg agccagttgt ctcaattgct ccaagccctg
    21001 ctcggtcaac aaatgaggct caaacaaggc cttgacctta tcgaactcta atgtttctaa
    21061 tattttctta ttcatcttta tttccttgtc tttgaatgtc taggtgtggt agctttttac
    21121 attctgatag attcgagtca aatctgtaaa caaagggcta ggaaaactcc tgcccttttt
    21181 atccgattaa atttgtcacc cagatttgtt tgagccaact ggttgttacc ggtatgctct
    21241 ggatgatgtg ttttgcgacg atactctttt caagaggatt ttgtataact gccatgggga
    21301 tggtcgccaa gattgtcaag gccatttgca agacaaataa ggtcaccaac atggacaaga
    21361 tacctgctga aacttggaac aacttaccac ccagtttttt gctaggaatc aagtgtaaga
    21421 gaagaccaag taaacgacca atgctataga caatcccaaa tacaagcaag tagccgatac
    21481 ctgcataaaa gaccttatcc agctgaaaga gttgatccga tgggaaaaag aaagtcccct
    21541 gaccttcctg cgaatttgca taagggagca ataaatggaa ttgctctcca agccccttat
    21601 aaaactggcc agccataaaa gccgatgcca tggctgaaat caggtaataa acctgtaaga
    21661 gcaggcctct ccgatagccg atataaaatc cccaaaccaa gaccaataga agaaggaatg
    21721 aaatcataag gaatcctcaa tcttgctctg ttcttgctta caagtcacaa gcttgtgacg
    21781 gagttcttct agctcttgct ccttatcgtc aaattcaatc tcacggctga gctgagttga
    21841 taaacagttg actgccaaca aaagagcgat tgtttcatca tctgcgctag gcatttgttc
    21901 tttaattgct tggtattttt ctgtcgcaac cttagcgatt tcctccataa aaaggttgtc
    21961 atgttcgctt gtcaaggtta acgatttttt cccgaatgta aatttgaatc gatttagatt
    22021 tgccataaaa ttcacctcac gatattatac caaaattcgc taattttgtc agtttttaca
    22081 aattttactg cttttgtggt acaatagaaa ctatggcaag tatcacactc acaccaagcg
    22141 aaaaggatat tcaggctttt cttgaacact atcaaaccag tctggctcct agcaagaatc
    22201 cctatatccg ctactttttg aaactacctc aagcaacggt ttctatctat acttctggaa
    22261 aaatcttgct tcagggtgaa ggggctgaaa aatacgccag tttctttggc tatcaagctg
    22321 tagagcaaac cagcggacaa aatcttcctt taattgggac agatgaggtg ggaaatggtt
    22381 cctactttgg tgggcttgca gttgtggctg cctttgtcac acctgaccag cacgactttt
    22441 tacgaaaact cggtgtgggg gattctaaga ctctgaccga ccaaaagatc cgtcagatta
    22501 ctcctattct caaggaaaaa attcagcacc aggcactcct tctctcaccc agcaagtaca
    22561 acgaggtcat cggagaccgc tacaacgctg tttcggttaa ggttgccctc cataatcagg
    22621 ctatctatct cctccttcaa aaaggtgttc agcctgagaa aattgtgatt gatgccttta
    22681 ccagtgctaa aaattatgac aagtacttgg cacaagaggc caatcgtttc agcaatccta
    22741 tcagcttaga agaaaaggct gagggcaaat acttgtctgt cgcagtttct tctgtcattg
    22801 cgcgtgatct ctttctggaa aatcttgaaa atttgggacg agaactgggt tatcagctcc
    22861 caagtggagc tggaacggct tctgacaagg tggctagcca gattttgcaa gcctatggta
    22921 tgcagggact cagcttctgc gccaaattgc actttaaaaa cactgaaaaa gcgaaaaaac
    22981 gcttagaaag gtaagttatg aattcattta aaaatttctt aaaagagtgg ggattgttcc
    23041 tcctaattct gtcattacta gctttaagtc gtatcttttt ttggagcaat gttcgcgtag
    23101 aaggacattc catggatccg accctagcgg atggcgaaat tctcttcgtt gtaaaacacc
    23161 ttcctattga ccgttttgat atcgtggtgg cccatgagga agatggcaat aaggacatcg
    23221 tcaagcgcgt gattggaatg cctggcgaca ccattcgtta cgaaaatgat aaactctaca
    23281 tcaatgacaa agaaacggac gagccttatc tagcagacta tatcaaacgc ttcaaggatg
    23341 acaaactcca aagcacttac tcaggcaagg gctttgaagg aaataaagga actttcttta
    23401 gaagtatcgc tcaaaaagct caagccttca cagttgatgt caactacaac accaacttta
    23461 gctttactgt tccagaagga gaataccttc tcctcggaga tgaccgcttg gtttcgagcg
    23521 acagccgcca cgtaggtacc ttcaaagcaa aagatatcac aggggaagct aaattccgct
    23581 tatggccaat cacccgtatc ggaacatttt aagaaaccta agaggcagag aatcaccaat
    23641 ctcagcctct tcttctatcg tgagaagtga ttgtttattt acaaatactc aatgaaaatc
    23701 aaagaacaaa ctaggaagct agccgtaggc tgcttaaaac agtgttttga ggttgtggat
    23761 agaactgacg aagtcagctc aaaatactgt tttgaggttg cagatggaag ctgacgtggt
    23821 ttgaagatat tttcgaagag tataaaataa aaacaatccc aactctcacc tattcatgca
    23881 aaggaatttt atggaagttt atttttcagg aactattgaa cggattattt ttgaaaatcc
    23941 cagcaatttt tatcgcatcc tcctcctaga aatcgacgat acggacgcag aggattttga
    24001 tgattttgaa atcattgtca caggaaccat ggctgatgta attgagggcg aagactatac
    24061 tttttggggg caaattgtcc agcactccaa gtatggagaa caactgcaaa tcagtcgtta
    24121 tgatcgcgca aaaccaacta gtaagggctt ggtcaagtac ttttcaagta gccatttcaa
    24181 ggggattggt ctcaagacag ctcagaaaat cgtggatacc tatggcgaaa ataccattga
    24241 cgaaattttg caacacccag aaaagttaga aggcatcgca ggactctctg ccaaaaatcg
    24301 cgaggctttc gtctccactc tccgtctcaa ctacggaacg gagatgattt tggccaaact
    24361 agccaactac ggcattccca acaaactagc ctttcagatt caagactttt acaaggaaga
    24421 aacccttgat gtggttgaaa attatcccta ccagctggtt gaggatatca agggtttggg
    24481 ctttaccatt gctgaccaac tagctgagga actaggcatc gaaagtcagg ctcctgaacg
    24541 cttccgcgcc ggtctagttc acagtctttt tcaggcctgt atggaaacag gggacaccta
    24601 tgttgaagca cgggatttgc tggaacaaac ccttactctc cttgagtctt cccgtcccgt
    24661 ggaactagac cccagccaag ttgcccaaga gctctcctac ctgatcgaag aagacaaggt
    24721 tcagcagatt gataccaaga tctttgacaa cagcctcttt ttcgctgagg aaggcatccg
    24781 cagtcacttg gttcgtatcc ttgaaaaagg aaaacagaag agtcatgatt tagaaaccat
    24841 tcaaaaacat atcactactg tcgagcaaga actgggaatt gagtatgata acattcaaaa
    24901 acaggctatc tgtgatgcta tccagaataa ggtctttatc ctgacaggtg ggcctggtac
    24961 tggtaagaca actgttatca atggaatcat tgctgtttat gcccttttag aaggacttga
    25021 cttcaggaag aaaagcaatc tgcccattct tcttgctgct ccaactggtc gagctgctcg
    25081 tcgcatgaat gaattgacag gtttgcctag cgcgaccata catcgccact tgggaatgac
    25141 aggtgacgat gataccagtc atctggaaga ttacctagat gctgacttta tcatcgtgga
    25201 tgaattctct atggtagata cttggctggc caatcaactc ttctccaata tctcttctaa
    25261 cagtaagatc ctcatcgtag gtgacagtga tcagctacct tctgtcagtc ctggacaggt
    25321 tctagcggat ctacttcata ttcctttgat tcctcagact cgcttggaaa aaatttatcg
    25381 acaaagcaaa gaatcaacca tcgtcaccct agctagtcag attcgacagg gcatcttgcc
    25441 agctgatttc acccaaaaaa aagctgaccg ttcctacttt gaaattgcta gtggccatat
    25501 tcctgccacc attgaaaaaa tcttaggtgc cgccctcaga aatggtattc ccgcccgtga
    25561 tatccaagtt ctggctccta tgtaccgagg gacggcaggg attgatgcta tcaatcagct
    25621 catgcaagac ctgctcaatc ccccacaaaa agatcaactc agttttgaag ctcctcagtg
    25681 tcactatcgt aagagagaca gggtcattca tttggttaac gatgctgaaa tcaatgtctt
    25741 taatggggat ttaggagcta tcacagacct gattcctggt aaatacaccg aatcgaaaca
    25801 agacgagatt gtcattgatt ttgatggtaa tgaagtctct tacccccgta acgaatggta
    25861 caagattcgc ttggcctatg ccatgagtat tcataagtct cagggaagtg agttcccagt
    25921 tgtcatccta ccgattacta gtgctagccg acgtatgctg gagcgaaatc tcatctacac
    25981 agccattaca cgtgccaaaa gcaaactcat cttactaggc gaattacaag ccttcgacta
    26041 tgctacccaa cacatcggaa ctgcccgaaa aacctatctg attgaacgct tcagtgatct
    26101 actggagaat gttgaagaaa agcaacaagc tgtctctgaa acagtcacat caagtgcctc
    26161 tgaacaatcc tacatcctaa ccgaagaaaa ctgggaccgc atcccagcca tgattgggat
    26221 tacagacaca gacctcaaag agatttttgg aaaatagtgc atcagaaaac ccaccaattc
    26281 aggtgggttt tctgtttatt aagattattt tactgttgct gtgcttgtga tcaattcaac
    26341 agcttttttg atagtgatat catgtttcaa catgtcagct gaaagcaagt tttgaacttg
    26401 tgcaacttcc atgttgtagt ctgctgccaa ttgctcaact tctttttgga tttcttcttc
    26461 tgaagcatca aatccttcag ctttggcaac tgcttcgata acaaggttag tcttagtacg
    26521 tgactcagct tctgcttggt attggttgtg aaggtcttct tgagtagttc cagtgatttg
    26581 gaagtacatg tcagggttga tcccttgacg ttgcaaattc ccaaggaatt catttactga
    26641 acggtgaact tcttcatgga tcatttcttc tggaagttct acgatttcag cattttctac
    26701 agctgtatca attgctgcac cttcaactgc atctttgtaa gcttcttctt tagcagcagc
    26761 caattctttg ctgtattttt ctttcaagtc agcaagtgtt tcaacttctt catcaatgtc
    26821 ttttgcaagt tcatcgtcaa gagccggaac ttctttagct tttacttcgt ggatagttgt
    26881 cacgaattta gcttctttac ctgcaaggtc ttctgcttgg tagtcttctg ggaatgttac
    26941 gataacatca acggtttcgc cagctgagtg acctaccaat tggtcttcga aaccagggat
    27001 gaattgacct gaaccaagtc caagtgagaa gttttcacct tttccaccgt caaattcaac
    27061 accgtcgata gaaccaacga agtcgatcac aacagtgtcg ccgttttcag cagcagcttc
    27121 cttgataacc aattcagcca ggttgttgcg ttcgcgttcg atacgctctt cgacatcagc
    27181 gtcagttact tctttttcta catcaactga tacttcaagg tttttgtagt cacccaattt
    27241 tacttcaggt tttgtaacga cttcagcagt gataacccag tcttgacctt tttccattga
    27301 agttacgtca atttttggtt gggcaaccac ttcaagacca gcttctttta cagctgcttc
    27361 ataagcgttt ggcaaaagtg cgttcattgc atcttgataa agagcttctt caccaaattt
    27421 ttggtcgaag atagggcgtg gaaggtgacc tttacggaaa cctggaacat taagagattt
    27481 cttcactgac ttgaagacac ggtccaattc tggtttgatt tggtcttgag agatagtgaa
    27541 agtcaagaca ccacggtttg tttctttgtt ttcaaatgat acagacattc tgtcatttct
    27601 ccttaaaatt ttttaaatac agtctattat aacattttct ataaataaaa tctagactaa
    27661 tgcagacaat tatagacaac aagccttttt cctaactaaa acactcataa agtatagaca
    27721 attttagact actttggata tcttttggtt agtcattttc caaaactaat ttatctaatt
    27781 tttcagttat atagtagatt gaaactagaa tagtacacct ctacttctaa aacattttta
    27841 gaaatcgatt tgactgtcct gatcgatttg tcctgttctt gtttcatttt actatataga
    27901 tcgcattcga aagaattcgt tccaaaattg cttggttcgt tcttctcttg gacgactaaa
    27961 gaattgatgg gcattatttt tttcaacaat tttcccactg tccataaaaa taacatgatg
    28021 ggctacatct ctagcaagac ctatctcata aagctgatca aaaactcaat ctgaggagaa
    28081 aatccaataa ggctttccat aataaaggac atcctatcaa gatttttgaa cacccgatag
    28141 gctgcctttt cccgattttg aagacttttt gtccagcttc ttttacctta taaataacaa
    28201 caaaaatcaa agacaagcta gaaagattac cgcagataac tcaaacagtg ttttgagatt
    28261 gcagataaga ctgacggagt cagctcaaag cactgctttg aggttgtaga tagaacaaca
    28321 tatgcctaag acaaagcgat gctaacctga tttgaagaga ttttcgaaaa agattaaata
    28381 tactctacaa tttgctcaat caattcttgg tcgtctaaac ctgtaacttg tataacaaca
    28441 tctttgactg attgtttagc caacaattca cctaatcgaa tactttcttc atcatttaca
    28501 tcgcgatagt caaagacata gccaactgtt ttaagtaggt ttttatatga caaactgagt
    28561 tctttcaatt cacgtatcgg ccggatgaat cgttcattat agcctaattt tcggattgga
    28621 gtacgagcta cgcgactaac ctcgtccact atgaaagggt tttcaagtcg ttctataatg
    28681 actttgtgat aattctccaa ttctttttta tcaaagttcc atttggcaat caagagactc
    28741 cgaatttcag ctaatacaga ttcaatccga gatttaatat taggattttg aagagcttcc
    28801 aaaattgtct tggcaccata atgcgcacca atgtaagctg aagttgcatg tccagaattg
    28861 actgaaaaaa gttttcgctc aataaagggt tctaaatctt cttcataatg cacatctttt
    28921 agacgtaaat ctggattttt aagacgcttg gtttccacga cccattcatt aaagggctcg
    28981 accacaacaa aaagggaatc ttcgtgactt tgtgctggaa caatcctgtc tactgcagca
    29041 tttggaaaac ctatgtagtt atcagcaaat gtcaaacctt ccggacttaa atatttcttg
    29101 acttcttgat aaagaaattg agacccgcca atcatatttt cacaggccat aacatccaat
    29161 gcctgtgtat ttcctgcaac tcggcgagct tcgattcctt tggctagaag ttcggcgata
    29221 aaagggagta tattaggtcc gattgcagta gtaataatat ccgtcttttg aatcgcttca
    29281 atgacttgct caggatgttc tttgctatta atgccagcca cattagttac ttctatacga
    29341 gactgtcctt tctgtgcaat ttcaatttca tacttgccct tttcattcag agcatgaatt
    29401 atctgattat tgacatccac aaaatcaata tggaaaccat ttttaaatag aatttcacct
    29461 ataaaaccac gaccgatatt accggcacca aaatgaacag aatgtttcat tcaatttctc
    29521 cttttcctat ttgagggaca tatatactag atgacaaatg actaaattca attattcaac
    29581 agcatcaaat aagcgcaata cttcctcttt tgactgagca tctgctagtt taagaacatt
    29641 atctacatct gcacagaaga tagaaatttt ctgaataatt tctaagtgtt cattaccaat
    29701 accagcaata ccaaaaagaa ccgttgccac ttgagggtta gatacattcc caaaatcaac
    29761 ccctctaggg acttgaacga ctgtaatacc agacttgagg acatcatttt ttgctgcatc
    29821 tgttccatgc ggtatggcga taaagttacc catgtaaaca gatagctctt tatctcgctc
    29881 aatcatggct tcaatatagt cttcattaac atatccaccc tcataaagaa cttgcccaca
    29941 ataacaaata gcttcctcct tgttagaaaa ttgtttatta agcttaatca aatgtttttc
    30001 aagtttcata ttagttctcc aatttcttaa ttttttcgtt aaaaatagta tttagcatct
    30061 gataaataat ggattgattt cccgtcttgt aaatctctgt ataaagatga ttttcaataa
    30121 tcgactgact aatagctgtc attaaatctc tagcttcctc gctagcttct ttagacatta
    30181 gcattagcag acaccttttg acctcttctt cctctcgttt cattgacaat gcggagatag
    30241 gtagtttgag ttcaaacata gtaaaactat ttgttgttac tttgctagac tgactatgta
    30301 aaagcaccag accagtatta ggaatagcca taggactatt ctggaagtga gataacaatt
    30361 ttcttgttag gtaagctctg tcactcacat tcttctgata ttggatgatt tgttctaccg
    30421 tgtgctcaaa tgattgatta ttctccaatt gaaccaactc gaattgactc aaaagctgac
    30481 tactagatat taaataatct tgcaagtcat aacttttctc tggagcgata gcatcacgcg
    30541 cgacaattgt acgatgtgcc tgtacatact gaagtcgttc ctgtaattca agcaattctt
    30601 tgacggttgg aaaactagaa attacatcga tttcctgatt agtcagcggc ttggtagata
    30661 aaatataatc atactgactg agatcaatac tgtggtaatc tactagagat tgaatatcta
    30721 tcaactctac aaaaggagca acactcttaa ttttactgac taataaatct ctagtgaccc
    30781 gacgttcatc cgttaaaagc aaaacacgga ctggataaat ctctggacta cggcctaagc
    30841 tagagataaa atgtagggca atcatgccat actcatactc tgtatgaaga tatttcggaa
    30901 aaatatcatt cactaaaaga ctgatgactg tataaagaaa tttattcctt tcaactaaaa
    30961 tctggataga ttctggcaaa ttttcaccct gaaaaaggat agggacgcct aaactgagcc
    31021 gaatatgatg gaaaagaaaa ttgaataaaa ccttgtcctt aaaaaagtca atcttggtat
    31081 acatggaaac cgtatcaatc agatttgaaa tattgtagaa aaattcacca tcaaatttct
    31141 ccgtaaagag cggattgtcc tgacgtttaa tgattaattc atccaagatg ctcgcaaaat
    31201 agataatttc ctgaatacta taaaattgtg cagtctgctt agagtaagct tgaaaaattt
    31261 tttgagaaat ttccaaagcc tgcttactag tattaggtga attttcaatg ttttgctcct
    31321 gacctataag agataacaag atcgcaaaaa acatcttcat cctagcatcc atatctggaa
    31381 aacctgacag ttgcttatta acaatctgac tggccagccc agttctatct gcttctaaaa
    31441 tatcaaagct cccaaaatta ccggttgaaa aatctgctac tgagatacag tttgtcagta
    31501 aaatagccaa aaatcttctc ttaccaactg aatcaccaga aatccgataa cctttttgtc
    31561 gttcaatttt cagatcaaag tctaaaagac gcttatcaat atctgaaata tcctgaataa
    31621 tagttacatt actgactttc gtgcactctt gcaatgcttc attggtgaca aaaccactct
    31681 cagtcagaag gcgataggta atcaactctt gtctttcttg gggactatac tcaactaaca
    31741 cttcaagttc tgtcggcaaa tcatccaact ctccagtcaa aatatagtat ttcccatgct
    31801 tcgttatttc gattccatat tgctccatgc tatctgtcaa atctgataaa gttcgataaa
    31861 ttgttctaga tgaaacctgt aagatttcag tcatatcttg tattgaaagc ttccctacat
    31921 gtaggaaagc cttcaataat tgttcttctc gtttggttaa tagcattcgt aatttagaaa
    31981 cctctagttc tatttgtaca ttctagcagc cattttgtca tattctggtg ttgttactaa
    32041 actatccaca ataagaaatt gagaattcgg tgctgcttgc tgcacttctg cctgtaaaga
    32101 aatagttgtt acaatcatta tgtttttaga attaaattca cctaattctg aaattttggc
    32161 agtagaaact ggaatacgaa tattcttgtt tctaaaaata gcccgaatcg tttcacagcc
    32221 catagttgca gttccctgtg ctttaccata agcaactact acagcatcaa tatggttaag
    32281 gtcactttcc tgactatcta ctggtgctga agttggtata tctccttcaa tttctgctat
    32341 tggagaagct cctgttaatg aagctacaat ttcatcataa cgagaggacg ctaagaaatt
    32401 atcaacagaa acatgaatag cacttggact cttgtcttta gctcttggtg tcagttcttc
    32461 ctgagtaaca attaatgttt ttggtgtatc aagcaaattt gagattgcct gattagatac
    32521 tggaatctct agacctgctt ttttaacctt atctcgaaga atactagctc ccatagcaga
    32581 gcttcccata ccagcatcgc aggcgaaaat gattttttcc actgagtctg tcgaaacaac
    32641 tgcatcaaca gaagttgata ctaactgacc tttagactga gccttagctg cttgggtagc
    32701 cgcctgagca gcttcgagcg aatcctcagt tgacttgtct gcatgaagaa taagggctgc
    32761 tacaaggaaa gaaacaactg ctgccactaa aacacctaaa agaacattta gatggggcca
    32821 aacacctttt ggcgccgtag ctataatcgc aataatagaa cctggtgaag ctggagattt
    32881 aagaccagcg tctaagagtt gaaaagtaaa agttccagag atacctcctg ccatagcagc
    32941 taaaaataga gtaggcttca tcataacata aggaaagtaa atttcatgaa tccctccgaa
    33001 gaaatgaata accattgccc cccaagaaga agatttagca gaacctttac cgaatacagc
    33061 ataagctaat agaattccca gacctggtcc aggattagct tccaatagga agagaattga
    33121 cttaccagct tgagctacct gttctactcc cagaggagta aaaatgccat gattgagggc
    33181 attattgagg aaaaggactt tagccggttc gatgataata ttagccatag gaaggaggcg
    33241 agcattgaca atagcctcaa caccattccc aacagctcca gtaagagtcg atacgactgg
    33301 accgattgcg tagaaagcca aaagcaataa tgcaaaacca acgagaccag ctgagaagtt
    33361 attaactaac atttcaaatc cgggacgaat tttttcctgg aacttctcat caaatttctt
    33421 gatagtccat cctcccagtg ggcccattac catagctccg ataaacatag gaacactaga
    33481 acctgtgatt gcaccaacag tagcaatagc tcctacaacg gcaccacgtt ggccatggat
    33541 catatatcca cctgtgtaac caatcaggat tggcaataaa tacgttaaca taggaccaac
    33601 aacagtagct aactgttcat ttggcagata gccatcagcg ataaagaggg cagtcaatac
    33661 tccccaagca ataaatgctc caatattggg cataaccata tttgaaagcg atgtccctag
    33721 tttttgaacc ctgactttca atgatacttt ttcttccatt agatcatcct ccttttctta
    33781 aaactagtat atacaaagtt aaaaaccttt aaaactaaag ttttgtcaca gattttgtgt
    33841 caaaacatta cttttgacga ggctgggcaa aaaggcttaa aaattataaa acgcatcata
    33901 tcaggtgtac atgaactac
//
LOCUS       DAVKNL010000020        33761 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVKNL010000020 DAVKNL010000000
VERSION     DAVKNL010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33761
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.020"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            <1..379
                     /locus_tag="VW411_000931"
     CDS             <1..379
                     /locus_tag="VW411_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEU9793687.1"
                     /translation="RNKNGQADTNQTEKPSEEKPQTEKPEEETPREEKPQSEKPESPK
                     PTEEPEESPEESEEPQVETEKVEEKLREAEDLLGKIQDPIIKSNAKETLTGLKNNLLF
                     GTQDNNTIMAEAEKLLALLKESK"
     gene            801..1175
                     /locus_tag="VW411_000932"
     CDS             801..1175
                     /locus_tag="VW411_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002960428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEU9793688.1"
                     /translation="MESIGLVIVSHSKHIAEGVVELISKVAKDVPITYVRGTEGGGIG
                     TSFEQVDRVVSENPADTLLAFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAYNAAA
                     LLQAGAELSVIQTQLAELEINK"
     gene            1635..2348
                     /locus_tag="VW411_000933"
     CDS             1635..2348
                     /locus_tag="VW411_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEU9793689.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFAHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYNLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            2406..3008
                     /locus_tag="VW411_000934"
     CDS             2406..3008
                     /locus_tag="VW411_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793690.1"
                     /translation="MSEVDFNEAVNYEFTSDTCQLANSIYQSLFKFFDKKNFSGDLIF
                     TWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLNMNRNGCMGDFPHDFF
                     DIYLDHVAKYAYEQKVNNIKEYYPLKRAILHQENALYFRFFSNFDDFLEKNYLKTIWQ
                     VSKETPFSEMDFNMFKNISEKIIFERGSKMLNDLKSNYKK"
     gene            3146..3799
                     /locus_tag="VW411_000935"
     CDS             3146..3799
                     /locus_tag="VW411_000935"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU9793691.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            3809..4273
                     /locus_tag="VW411_000936"
     CDS             3809..4273
                     /locus_tag="VW411_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9793692.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            4286..5554
                     /locus_tag="VW411_000937"
     CDS             4286..5554
                     /locus_tag="VW411_000937"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU9793693.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIASEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLS
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRFVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            5568..5828
                     /locus_tag="VW411_000938"
     CDS             5568..5828
                     /locus_tag="VW411_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793694.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            5839..7200
                     /gene="pdrM"
                     /locus_tag="VW411_000939"
     CDS             5839..7200
                     /gene="pdrM"
                     /locus_tag="VW411_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEU9793695.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPIILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSPLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTVRFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLSK
                     SKC"
     gene            7354..8322
                     /locus_tag="VW411_000940"
     CDS             7354..8322
                     /locus_tag="VW411_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU9793696.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            8338..9330
                     /locus_tag="VW411_000941"
     CDS             8338..9330
                     /locus_tag="VW411_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEU9793697.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            9548..10591
                     /locus_tag="VW411_000942"
     CDS             9548..10591
                     /locus_tag="VW411_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEU9793698.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGKIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            10655..12340
                     /gene="lpdA"
                     /locus_tag="VW411_000943"
     CDS             10655..12340
                     /gene="lpdA"
                     /locus_tag="VW411_000943"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU9793699.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQFGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVNKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            12404..13393
                     /locus_tag="VW411_000944"
     CDS             12404..13393
                     /locus_tag="VW411_000944"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEU9793700.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGIKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(13458..14528)
                     /gene="xerS"
                     /locus_tag="VW411_000945"
     CDS             complement(13458..14528)
                     /gene="xerS"
                     /locus_tag="VW411_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEU9793701.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGCKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVSDEQKNALDSL"
     gene            complement(15112..16662)
                     /locus_tag="VW411_000946"
     CDS             complement(15112..16662)
                     /locus_tag="VW411_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU9793702.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
     gene            complement(16678..17457)
                     /locus_tag="VW411_000947"
     CDS             complement(16678..17457)
                     /locus_tag="VW411_000947"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU9793703.1"
                     /translation="MATIKEIKEFLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILSKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELLKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            complement(17444..18295)
                     /gene="ylqF"
                     /locus_tag="VW411_000948"
     CDS             complement(17444..18295)
                     /gene="ylqF"
                     /locus_tag="VW411_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEU9793704.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            complement(18514..18690)
                     /locus_tag="VW411_000949"
     CDS             complement(18514..18690)
                     /locus_tag="VW411_000949"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9793705.1"
                     /translation="MQAGIPTDNEVSYDLIYQQETLPATGSSTSELTALGLLAVGSLV
                     LLVHNMTGTVFCSL"
     gene            complement(18742..24756)
                     /locus_tag="VW411_000950"
     CDS             complement(18742..24756)
                     /locus_tag="VW411_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9793706.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAAMGILIFAVSKKKVKNKTVLHLVLVAGIGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSESEVSNQKSSVATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETMETKIDFQEEI
                     QENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPSPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGAIVEPAIQPELPEAVVSDKGEPEVQPTLPEAVVTDKG
                     ETEVQPESPDTVVSDKGEPEQVAPLPEYKGNIEQVKPETPVEKTKEQGPEKTEEVPVK
                     PTEETPVNPNEGTTEGTSIQEAENPVQPAEESTTNSEKVSPDTSSKNTGEVSSNPSDS
                     TTSVGESNKPEHNDSKNENSEKTVEEVPVNPNEGTVEGTSNQETEKPVQPAEETQTNS
                     GKIANENTGEVSNKPSDSKPPVEESNQPEKNGTATKPENSGNTTSENGQTEPEPSNGN
                     STEDVSTESNTSNSNGNEEIKQENELDPDKKVEEPEKTLELRNVSDLELYSLSNGTYK
                     QHISLEQVPSNPNSYFVKVKSSSFKDVYLPVASISEERKNDKILYKITAKVEKLQQEI
                     ESRYKDNFTFYLAKKGTEETTNFTSFSNLVKAINQNPSGTYHLAASLNANEVELGPDE
                     RSYIKDTFTGRLIGEKDGKNYAIYNLKKPLFENLSGATVEKLSLKNVAISGKDDIGSL
                     ANEAQNNTKIKQVHVDGVLAGERGIGGLLAKAEQSSITESSFKGRIINTYETTAAYNI
                     GGMVGHLTGDKALLTKSKATVAISSNTNTSDQTVGGLAGLVDRDAQIQDSYAEGDINN
                     VKHFGRVAGVAGNLWDRTSGDVRHAGSLTNVLSDVNVTNGNAITGYHYNEMKVKDTFS
                     SKANRVYNVTLVKDEVVSKESFEERGTMLDASQIASKKAEINPLILPTVEPLSTSGKK
                     DSDFSKVAYYQAKRNLTYKNIEKLLPFYNKATIVKYGNLVNENSLLYQKELLSAVMMK
                     DNQVITDIVSNKQTANKLLLHYKDDLSEKLDLKYQNDFAKLAEYSLGNTGLLYTPNQF
                     LYDQTSIIKQVLPDLQKVDYHSEAIRKTLGISPNVKQTELYLEDQFAKTKQQLEDSLK
                     KLLSADAGLASANPVTEGYLVDKIKRNKEALLLGLTYLERWYNFSYGQVNVKDLVLYH
                     LDFFGKGNASPLDTLIELGKSGFNNLLAKNNVDTYGISLASQHGTTDLFSTLEHYRKV
                     FLPNTSNNDWFKSETKAYIVEEKSTIEEVKTKQGLAGTKYSIGVYDRITSATWKYRNM
                     VLPLLTLPERSVFVISTMSSLGFGAYDRYRSSDHKAGKALNDFVEENARETAKRQRDH
                     YDYWYRILDDNAREKLYRNILLYDAYKFGDDNTVGKATEVADFDNPNPAMQHFFGPVG
                     NKVGHNQHGAYATGDAVYYMGYRMLDKDGAITYTHEMTHDSDQDIYLGGYGRRSGLGP
                     EFFAKGLLQAPDHPDDATITINSILKHSKSDSTESRRLQVLDPTTRFNNADDLKQYVH
                     NMFDVVYMLEYLEGNSILKLDTNQKQQLLRKVTNEYHPDPDGNKVYATNVVRNLTVEE
                     VERLRSFNDLIDNNILSSREYASGKYERNGYFTIKLFAPIYAALSNDIGTPGDLMGRR
                     IAYELLAAKGFKDGMVPYISNQYEEEAKQKGKTINLYGKTRGLVTDDLVLEKVFNNQY
                     HTWSEFKKAMYQERQDQFDRLNKVTFNDTTQPWQTFAKKTTSSVDELQKLMDVAVRKD
                     AEHNYYHWNNYNPDIDSEVHKLKRAIFKAYLDQTNDFRSSIFENKK"
     gene            complement(24965..26143)
                     /locus_tag="VW411_000951"
     CDS             complement(24965..26143)
                     /locus_tag="VW411_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793707.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSFIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSNFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWILNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
     gene            complement(26358..30008)
                     /gene="addA"
                     /locus_tag="VW411_000952"
     CDS             complement(26358..30008)
                     /gene="addA"
                     /locus_tag="VW411_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEU9793708.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSAQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLILE
                     NEVFHEVFEAHYQGKQKETFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQESDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FAKLGQLSDQIAILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGRLIILKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNKHQLVFANTKLTPNPDNKAAFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLESKEYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTVLGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            complement(30005..33280)
                     /gene="rexB"
                     /locus_tag="VW411_000953"
     CDS             complement(30005..33280)
                     /gene="rexB"
                     /locus_tag="VW411_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEU9793709.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLEYLSQQASFSITVTRFAQMARYLVLNDLPAKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQESQLSHLIEAIENDKVSSDFNQIALVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSVRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAEKLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDQQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLHPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWLEKIREE
                     LNR"
     gene            complement(33480..33635)
                     /locus_tag="VW411_000954"
                     /pseudo
     CDS             complement(33480..33635)
                     /locus_tag="VW411_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
BASE COUNT         9661 a         6587 c         6912 g        10601 t
ORIGIN      
        1 aagaaacaaa aatggtcaag ctgataccaa tcaaacggaa aaaccaagcg aggagaaacc
       61 tcagacagaa aaacctgagg aagaaacccc tcgagaagag aaaccgcaaa gcgagaaacc
      121 agagtctcca aaaccaacag aggaaccaga agaatcacca gaggaatcag aagaacctca
      181 ggtcgagact gaaaaggttg aagaaaaact gagagaggct gaagatttac ttggaaaaat
      241 ccaggatcca attatcaagt ccaatgccaa agagactctc acaggattaa aaaataattt
      301 actatttggc acccaggaca acaatactat tatggcagaa gctgaaaaac tattggcttt
      361 attaaaggag agtaagtaaa ggtagcagca ttttctaact cctaaaaaca ggataggaga
      421 acgggaaaac gaaaaatgag agcagaatgt gagttctagt tctcattttt ttcatgaaaa
      481 tgtgcaaaat atagtagatt gaaactagaa tagtatacct ctacttctaa aacattgtta
      541 gaaatcgatt tgactgtcct gttcttattt cattttacta tatcttaaca gatagtgtaa
      601 ataaagataa actatttact ggctaattaa tcagttaaac actagttaag gagtaatgat
      661 gaaaaaaaga acaatactat tattgatggc cagtctgtta gctcttgtct taggagcatg
      721 tggtttcttg gacatattga tcctggatca ttctcatcag gattactctt tactgctatt
      781 ttagaaactg gggtggtttg atggaaagta ttggtcttgt tatcgtttca cattccaaac
      841 acattgcaga aggtgttgtt gaactgatta gtaaagtagc taaagatgtt ccgattactt
      901 atgtaagagg aaccgagggc ggaggaattg gaacgagttt tgaacaagta gatagggttg
      961 tttccgaaaa tccagcagat actttacttg ccttttttga cctaggttct gctaaaatga
     1021 acttaaaaat ggtgactgat ttcagtgata aaagtatcat catcaacagg gttccaattg
     1081 tagaaggtgc ctataatgca gctgctcttc ttcaggctgg tgcagaactg tcagttattc
     1141 aaacacagtt agcggagctt gaaatcaata aataaggaat tttactataa ctctttttat
     1201 agataagcta ttgattatct caactataaa atgttaagta aatgatttca aaatcagaaa
     1261 gggattgctt tatgcagttc ctttttattt taacaggagt gaaactatag tgtttctaaa
     1321 ttgtgaatca atcaaaactg attgtgatgg ggctattcta gctttagaaa ccttcaaaaa
     1381 ttaaaattta aggcaatcaa ttacttggaa gagtatgaaa gcatttagtt tataggaatt
     1441 ctaggtctag aattacatat atatatttat gaagacgggg tgttcgatag ttagtattgt
     1501 tctattctga aagatttgag ctgtcagttg tatagaaagt gttcgaattt ttttaagtga
     1561 ttaaattagt taattgtatg aggtgcttta tgatataatg ttcttaatga attttcagaa
     1621 aggaaaacct caaattgttc tacaaatttc tactcttcga cctcgaccac actcttcttg
     1681 attttgatgc tgctgaggat gtggctttga cccaacttct aaaagaagaa ggagttgcgg
     1741 atattcaggc ttataaagat tattacgttc ctatgaacaa ggctctctgg aaagacttgg
     1801 agctgaagaa aatcagtaaa caagagctgg ttaacacgcg cttttctcgt ttatttgctc
     1861 attttggaca ggaaaaagac ggtagttttc ttgcccagcg ttaccaattt tacctcgccc
     1921 agcagggaca aacactatcg ggcgctcatg atctcttgga cagcctcatt gagcgtgatt
     1981 ataacttgta tgctgcgaca aatggcatta ctgccattca gacaggacgt ttggctcaat
     2041 ctggtctagc accttatttc aatcaagtct ttatctcaga acagttgcaa actcaaaagc
     2101 cggatgctct tttttatgaa aagattggcc agcaaattgc tggatttagt aaagaaaaga
     2161 cgctgatgat tggagattct ctaaccgccg acattcaagg tggcaataat gcggggattg
     2221 acactatctg gtataatcct catcacctcg aaaatcacac acaagcccag ccgacttacg
     2281 aagtctattc ttaccaagac ttgctggatt gtttagataa aaatattctt gaaaagatca
     2341 cattttaaag gagacgagct aatgactaca aaaaagctaa tattactatt gaagagtaca
     2401 ttgaaatgtc tgaagttgat tttaatgaag ctgttaatta tgaatttaca tctgacactt
     2461 gtcaattagc aaatagtatt tatcaatctc tttttaagtt ttttgataag aaaaatttct
     2521 ctggcgattt aatttttact tggaaatctc catcattagt caaagaaggg gattatattg
     2581 ggagaaggga ttcacaagta gataatctta gagtaatagg aaatatattt ccgaattatc
     2641 ttactaatcg aaaatatagc ctcaatatga atcgtaatgg ctgtatggga gattttcctc
     2701 atgacttttt tgatatatac ctagatcatg tagcaaaata tgcctacgaa caaaaagtta
     2761 ataatattaa agagtattat cctttaaaaa gagcgatttt acaccaagag aatgcattgt
     2821 attttcgatt tttttctaat tttgacgact ttttagaaaa aaattattta aagactatat
     2881 ggcaagtttc taaagaaact cctttttctg agatggattt taatatgttt aaaaatattt
     2941 cagaaaagat aatcttcgaa agaggtagta aaatgcttaa tgatttaaaa agtaattata
     3001 aaaaatagcc agtctttata aactgtattc acgtattcaa atcgtatata tagtcgattt
     3061 attaaatctt tagataaact gtaaaaagtg gtttccatag ccactttttg ctataataga
     3121 ggcagtaaaa atgaaggagt cggctatgga acactcgtct tggcatgctt tgattaaggc
     3181 gcaattacct gagggttatt tcgggaaaat caatcagttt atggagcagg tctattctca
     3241 ggggattatt tatccaccca aggaaaaggt ttttcaggct ctcttgacaa cactgcttga
     3301 agaagttaag gtggtaattc tagggcaaga cccctatcac ggaccaggtc aagcgcaggg
     3361 cttgagtttt tctgtacctg actctatccc agctccacca tccttgcaaa atatcttgaa
     3421 agaattgtca gatgatatcg gagttaagaa atctcatgat ttgacagctt gggctgagca
     3481 aggagtcttg cttcttaatg cttgtttgac tgttcctgct ggacaggcca atggtcatgc
     3541 tggtcaaatc tgggagcctt ttactgatgc tgtgattcag gtggtcaatc atctagatag
     3601 accagtcgtt tttgtactct ggggagctta tgcacgtaag aagaaggcat tagttaccaa
     3661 tcctcatcac ttgattatcg aatcagccca tccaagtcct ttgtcggttt atagaggatt
     3721 ttggggttcc aagccctttt ccaaggccaa tgcattctta aaagagacag gacaagagcc
     3781 aatcgattgg cttagataag gagaaaatat gcctcagtta gcgacgattt gctacattga
     3841 taatgggaaa gaactgctca tgctccatcg taataagaaa cccaatgatg tccatgaagg
     3901 gaaatggatt ggtgtgggtg gtaagctaga gagaggagag acgccccagg aatgcgcggc
     3961 gcgtgaaatc cttgaagaaa cagggctcaa agccaagcca gttctaaaag gtgtcatcac
     4021 ttttcctgaa tttacaccag atttagactg gtacacctat gtttttaagg tgacggagtt
     4081 tgagggcgac ttgattgact gcaatgaggg gacgctagaa tgggttccct atgatgaggt
     4141 tttgagcaag ccgacttggg aaggtgacca cacctttgtt gagtggcttt tagaggataa
     4201 acccttcttt tcagccaagt ttgtttatga tggggataaa ttgttggata cccaagttga
     4261 tttctatgaa taaaggagaa agcagatgct actaatcaaa aatggtcgtg taatggatcc
     4321 caagtctggt ttggatcaag tttgtgatgt cttagttcaa gatgggaaaa ttatcaaaat
     4381 tgcgtctgag atcacggaag aaggagcaga aacgattgat gctactggtc ttgtggttgc
     4441 tcctggcttg gtcgatattc atgttcattt ccgtgaacct ggtcaaacac ataaagaaga
     4501 cattcatact ggtgccctag cagccgctgc aggtggtttt actactgtcg tcatgatggc
     4561 taataccagt ccaaccattt cagacgtgga gactttgcaa gcagttctcc agtcagctgc
     4621 caaagagaag attaatgtca agacagttgc gaccattact aaaaacttta atggtaaaaa
     4681 cttgactgac tttaaggcac tcttagaagc tggtgcggtt ggtttctctg atgacggtat
     4741 tccgcttgag agcagtaaga ttgtcaagga agccatggag gaagccaaaa aactcaatac
     4801 ctttatcagc cttcatgagg aagatccagg tttgaacggt gttcttggct ttaatgaaaa
     4861 tattgctaga gaacatttcc atatctgcgg tgctactggg gtggctgagt acgctatgat
     4921 ggcgcgtgat gtcatgattg cctatgcaac taaagcccat gttcacatcc agcatttgtc
     4981 taaggaagaa agtgttaaag tagtggagtt tgctcagggg ttaggtgcag aagtcacagc
     5041 agaagtagcg ccacagcatt tctctaagac agaagcactt cttttaacac aaggtagcaa
     5101 tgctaagatg aatccaccgc ttcgtttgga atcagaccgt cgtgccgtta tcgaaggtct
     5161 caaatcaggt gtcatcacag ttattgcgac tgaccacgcg cctcatcatg tagatgaaaa
     5221 aaatgttgag gatattacca aagcgccatc tggtatgact ggcttagaaa catccctgtc
     5281 tctcagcttg acctatttag tagaagctgg tgagttgagc ttgatggaat tacttgaaaa
     5341 aatgacatac aacccagcca agctttacaa ctttgaagca ggttacttgg ctgagaatgg
     5401 tccagcagat atcactattt ttgatgccaa ggctgaccgc tttgtggact cccattttgc
     5461 ttccaaagca gctaattcac cattcatcgg tgaaacctta aaagggcagg ttaaatatac
     5521 catctgtaag ggacaaatcg tctatcaagc ttgataggag ttagaatatg aattattttc
     5581 gaaaacgtag ggagagacaa gcgaaatcta atagtggaat ttactgtccc gcagctaatc
     5641 gttccaaaat tcaatatgaa actaaagaaa aagctgacca tgcggttcaa tatgataacg
     5701 gaggtttagt tagaagctat tattgtcgta catgtgcgtg ttggcatact acttcaaaat
     5761 ctaataaacc tccgctaagt ttgaaaaact atggtctgaa actctttggt ttagataaat
     5821 cagaatagaa agagaagtat gtataagaca aagtgtttac gagagaagtt agtattattt
     5881 ttaaaaattt tcttcccaat cctgatctac caatttgcca attattctgc ctcttttgtt
     5941 gatactgcaa tgacaggtca atacaacact atggacttgg ctggtgtatc tatggcaacc
     6001 agtatctgga atcctttctt tacatttcta acagggattg tgtcagcctt ggtgcctatc
     6061 attggtcacc atcttggtcg aggcaaaaag gaagaagttg cgtctgattt ttaccaattt
     6121 atttatttgg ccttgggcct atctgtggtc ttgctgggga tggtactttt cttggcacca
     6181 ataatcttga atcatattgg gttagaagca gcagtagcgg cagtagcggt tcgctatctt
     6241 tggtttttat ctatcgggat tatccccttg ttgctcttta gcgtcattcg ttccttgctg
     6301 gattcgctgg gcttgaccaa actgtccatg tacctcatgc ttttgttact ccctctcaat
     6361 agcggattta actatctctt gatttacggt gcctttggtg ttccagaact gggaggggct
     6421 ggtgctggtt taggaacatc cttggcctac tgggtcttgc ttgggatttc tgttctggtt
     6481 ttatttaaac aggagaagct caaagcctta caccttgaga aacgaattcc acttaatatg
     6541 gataaaatta aggaaggagt tcgtttaggt ctgcctattg ggggaactgt cttcgcggaa
     6601 gtggctatct tttcagtggt tggcttgatt atggctaagt tttcgccctt gattatagct
     6661 agtcaccagt cagctatgaa cttttcaagt cttatgtacg cctttcctat gagtatctca
     6721 tcggctatgg ctattgtcgt ttcctatgaa gtgggagcca agcgatttga tgatgcgaaa
     6781 acctatattg gtctaggaag atggactgcc ctcatttttg cggccttcac cttaaccttc
     6841 ctttacattt ttaggggaaa tgtggccagt ctttatggta acgacccaaa atttatcgat
     6901 ttgacagtgc gttttttaac ttatagtctt ttcttccagt tagcagatac ctttgcggcg
     6961 ccgcttcagg gaattttgcg ggggtataag gatacagtta ttccttttta ccttggtttg
     7021 cttggttatt ggggcgtagc aatccctgtg gctacgctat ttgattccct aacagatttt
     7081 ggagcttatt cttactggat cggcttgatt attagtttga ttgtgagcgg ggcgctctac
     7141 cgttggcgtt taactgtgat tatgaagaga tttgaatctt tatcaaaatc caaatgctaa
     7201 aaagtttgtg aaaaaatatt cttgacaaga aaatcccttg ttttcacggg gttttctttt
     7261 ttattttgtg aaatttcttt tagaaaaact ttgacagtat ttgccaaaag cggtaaaata
     7321 gtaaggtaag aagaaagtta gaaaggcaag aagatgtcaa ctttagataa aaatcttttg
     7381 ctagaaatgt tccgtaagat ggaagaaatc cgtcgcatgg acttaaaaat tgcacaatta
     7441 gtaaagaaag ggaaagtgcc aggaatgacg cacttttctg ttggtgaaga ggcagctaac
     7501 gtgggggcta tgttagctct caatccagat gatctgatta cctcaaacca ccgtggacac
     7561 gggcaagcta ttgctaaagg gattgacctc aacggaatga tggctgaaat ccttggtaaa
     7621 tacactggaa cctgtaaagg gaaaggtgga tctatgcata tcgctgacct tgatgctggg
     7681 aaccttggtg ccaatggtat cgtaggtggt ggtatgggga tcgctgtcgg tgcagccctc
     7741 agtcagcaaa tgcaaaatac cggtaaaatc gttgtctgct tctttggaga tggtgcgacc
     7801 aatgaaggtg ttttccacga agcagtgaac atggcttcta tctggaacct gccagtcatt
     7861 ttctattgca ttaacaacgg ttacggtatc tctgcggata tcaagaaaat gaccaatata
     7921 gaacatatcc atcaacgtag cgccgcttat ggaattcctg gaatgttcat cgaagacggt
     7981 aacaatgtca tcgatgtcta tgaaggattt cagaaagctg tggaccatgt tcgcagtggc
     8041 aatggtccag tcttgattga aagtgtaact tatcgctggc tcggtcactc atcatctgac
     8101 cctggtaaat atcgtacgcg tgaagaagtg gaattgtgga aacaaaaaga tccaatcgaa
     8161 aacctccgca attaccttat tgaaaataac attgcaagtg ccgaagaatt ggaagaaatc
     8221 caagcgcaag taaaggaagc agtagaagct tctgttaaat ttgcagagga aagcccattc
     8281 ccaccgcttg aatcagcctt tgaagatatt tacgcagact aaggagaaag agataaaatg
     8341 gaaacaaaaa caatgtcctt ccgtgacacc attatccttg ctatgtctga ggaaatgcgt
     8401 cgcgatgaaa atgtgttctt gatgggagaa gacgtcggtg tcttcggagg agacttcggt
     8461 acttctgttg gaatgcttga agaatttggt ccagaacgtg tccgtgactg tccgatttct
     8521 gaagctgcca tctctggagc agcagcagga gcagccatga caggacttcg tccaatcgtc
     8581 gatatgacct tcatggactt ctctgttatt gccatggaca atatcgtcaa ccaagctgct
     8641 aaaacacgtt acatgtttgg tggtaaaggt caggttccaa tgactgttcg ttgtgcagct
     8701 ggtaacggag ttggctctgc cgcccagcac tcacaatctc tagagtcttg gtttactcac
     8761 attccaggac ttaaggttgt ggcacctgga acacctgcgg acatgaaagg cctgcttaag
     8821 tcttctatcc gtgataacaa cccagttatt atacttgagt acaagtcaga atttaaccaa
     8881 aaaggggaag tgccagttga tccagactac acaattccac ttggggttgg tgaaatcaaa
     8941 cgccaaggaa cggatgtaac agttgttact tatggaaaaa tgcttcgccg tgtggttcaa
     9001 gctgctgaag aattagcaga agaaggaatt tcagttgaaa ttgttgaccc acgtaccctt
     9061 gttccgcttg ataaggatat catcataaac tcagtgaaga agactggtaa ggttgttctg
     9121 gtcaacgatg cccacaaaac aagtggctat atcggtgaaa tttcagctat tatttcagaa
     9181 tcagaagcat ttgactatct agatgcacca attcgccgtt gtgcaggaga agatgtgccg
     9241 atgccttatg cacaaaacct agaaaatgca atgattccaa cagttgaaag tatcaaagat
     9301 gcaatccgaa aaacttataa caaagaatag ggttacataa gataagttat gtaaataaaa
     9361 taaagctgac gagaaacttt gtatctttta ggtgcagagc tcttcgtcat taatatctta
     9421 gattagagca cgggctaaaa gatcggaaaa aagataaatc tccttggctt catcgaagcc
     9481 atcgtcgatt tcctattttt cagtcgcttt tgacgccctt tgtatcataa atagaattgg
     9541 agaggtcatg gctgatgaca agctaagagc gactcctgca gctagaaagt tagcggatga
     9601 tttagggata aacctctacg acgtttctgg ctcaggtgca aacggtcgtg tccacaaaga
     9661 agacgtggaa acttataaag acacaaacgt ggttcgcatt tcgccacttg caaaacgaat
     9721 tgccctcgaa cataacattg cttggcagga aatccaagga accggtcatc gtggtaaaat
     9781 catgaagaag gatgttttgg ccctgcttcc tgaaaatatc gaaaacgata gcatcaaatc
     9841 tcctgctcag attgaaaaag tggaagaagt ccctgataac gtaacaccat atggtaaaat
     9901 cgagcgtatt ccaatgacac caatgcgtaa ggttattgcc caacgcatgg ttgaatctta
     9961 cttgactgcg ccaaccttca ccctcaacta tgaagttgat atgactgaaa tgttggctct
    10021 tcgtaagaag gttcttgagc caatcatgga agcaactggg aagaagacta ctgtaacaga
    10081 ccttctttcg cttgcagttg ttaagactct tatgaaacac ccatacatca acgcttcatt
    10141 gacagaagat ggcaagacta ttatcactca caactatgtc aatcttgcca tggcagttgg
    10201 gatggataat ggattgatga cacctgttgt ttacaatgct gagaagatga gtctttcaga
    10261 actggttgta gcctttaagg atgttattgg ccgtaccttg gatggtaaat tggctccaag
    10321 tgagttacaa aattcaacat tcacaatcag taatttggga atgtttggtg ttcagtcctt
    10381 tggtccgatt attaaccaac ccaactcagc tatccttggt gtcagttcga caatcgagaa
    10441 gccagttgtc gtcaatggtg aaattgtgat tcgcccaatc atgagtttag gattaaccat
    10501 tgaccaccgt gtcgtagatg gtatggctgg tgctaagttt atgaaggact tgaaagagtt
    10561 gattgaaacg ccaatctcaa tgttaattta agagcaagag tattcatttt taagatatag
    10621 ataaaggaag gaagacatgg ccttagaagt aattatgcca aaagccggcg tggatatgac
    10681 agaaggacaa atcgtccaat ggaataaaaa agtcggagaa tttgtaaaag aaggagaaat
    10741 ccttttggaa atcatgactg ataaagtcag catggaattg gaagccgaag aagatgggta
    10801 cttgattgcc attctcaaag gagatggtga aactgtccct gtaacggaag ttatcggtta
    10861 ccttggtgaa gaaagggaaa acatcccaac agctggagca gcatcgccag aagctagccc
    10921 tgtacctgta gcaagtacct caaacgatga tggtaagagc gatgatgctt ttgatatcgt
    10981 tgtgattggt ggaggtcctg ctggttatgt tgcagccatt aaagctgccc aattcggcgg
    11041 taaggttgcc cttgttgaga aatctgaact tggtggaacc tgcttaaacc gtggatgtat
    11101 tccaaccaag acctaccttc ataacgctga aattattgaa aatatcggtc atgctgcaaa
    11161 tcgtggtatc gtgattgaaa atcctaactt cactgttgat atggaaaaac ttttagaaac
    11221 taaatctaaa gttgttaata ctcttgttgg tggtgttgca ggacttcttc gtagctacgg
    11281 agttactgtt cataaaggaa ttggtacaat cactaaagac aagaacgtct tggtaaatgg
    11341 ttctgaattg cttgaaacca agaaaatcat tcttgctggt ggttcaaaag tcaacaagat
    11401 caacgtccct ggtatggaat ctccacttgt catgactagt gatgacattc ttgaaatgaa
    11461 cgaagtgcca gaaagccttg ttatcatcgg tggtggagtt gtcggtatcg aactcggtca
    11521 ggccttcatg acatttggtt caaaagtgac tgttatcgaa atgatggacc gtatagttcc
    11581 agctatggat gtggaagttt ctaagaacct tcgcttgatt cttgagcgta aaggaatgac
    11641 catcttgact ggtactaaac tgcaagaaat cattgaggaa aatggtcaac ttcgtatcaa
    11701 ggttgaagga aaagacgata tcatcgcaag caaagctctt ctttcaattg gtcgtatgcc
    11761 agaccttgaa ggtattggag aggttgagtt tgaattggat cgtggttgta tcaaggtcaa
    11821 cgaatacatg gaaacttcag ttccaggcat ttatgcacca ggtgacatca acggtactaa
    11881 gatgttggct cacgcagctt tccgcatggg tgaagtttcc gctgaaaatg cccttaaagg
    11941 aaatcatgca gttgccaaat tgaatttgac tcctgcagcc atctacactc tccctgaagt
    12001 agcagcagta ggtttgacag aagaacaagc ccgtgagaaa tacgatgttg ccatcggtaa
    12061 gtttaacttt gctgctaacg gtcgtgctat tgcatctgac gcagctcaag gtttcgtaaa
    12121 agttatcgct gataagaaat acggagaaat ccttggtgta cacattattg gtcctgcagc
    12181 cgcagaatta ataaacgaag catcaagcat catcgaaatg gaaatcactg ttgaggaaat
    12241 gctgaagacc atccacgggc acccaactta ctctgaagtg atgtacgaag cgtttgcaga
    12301 tgttctagga atggccatcc attcacctaa gaaaaaataa aacgaagata gattagactg
    12361 gaaggttatt tccagtttct atcatataaa gtaaagggat atcatgaaat acattatcaa
    12421 tcattcaaat gacactgctt ttaatattgc cttggaagaa tacgccttta aacacctttt
    12481 ggatgaggat caaatcttcc tactttggat taacaagcca tctatcattg ttggtcgtca
    12541 ccagaacact atcgaagaaa tcaaccgtga ttatgttcgc gaaaatggta ttgaggtagt
    12601 tcgccgtatt agtggtggtg gagctgttta ccacgattta aataacctca actacacgat
    12661 catctccaaa gaagatgaaa ataaggcatt tgacttcaag agcttctcaa ctccggttat
    12721 caataccctg gctcaacttg gggttaaagc tgagttcaca ggtcgtaatg accttgagat
    12781 tgatggtaag aagttctgtg gcaatgccca agcctatatc aacggccgta tcatgcacca
    12841 cggttgcttg ctctttgacg ttgatttgtc agtcctcgca aatgccctta aggtttcaaa
    12901 agataaattt gaatcaaaag gtgtgaaatc cgtccgtgcc cgtgtaacca atattatcaa
    12961 tgaattacca aaaaaaatca cagtcgaaaa attccgtgat ttactcttgg aatacatgaa
    13021 aaaagagtac ccagagatga ctgaatacgt cttttcagaa gaagaattgg ccgaaatcaa
    13081 tcgcatcaag gatactaagt ttggaacttg ggactggaac tatggtaaat cacctgaatt
    13141 taacgtccgt cgtggaatca aattcactag tggtaaggtt gaagtctttg ctaacgttac
    13201 tgaatcaaaa atccaagaca tcaagattta tggtgacttc tttggtattg aagacgttgc
    13261 tgcagtagaa gatgtccttc gtggggtgaa atacgaacgc gaagatgttc ttaaggcgct
    13321 aaaaaccatt gatatcacac gctacttcgc tggtattagc cgtgaagaaa tcgctgaagc
    13381 ggtagttgga taaatcaaaa agaagttggc caacttcttt ttttgatatt tatttacata
    13441 atttaaaata cgtaaaatca taaactatcc agagcattct tttgttcatc actaacaata
    13501 tgggtataga ggtcagtgac ttgtgtgcta gcatgtccta gttggtgact gactaaaact
    13561 tgtgatttag tcgcatcata gagcctagtt gctagtgtat ggcgcagttt atggggtgtt
    13621 acacgcactt taaaatcctc tgagtattta gcaaccattt tctcaacgct agaagcatcg
    13681 atacgattag gaacacctct gtagagagtt aaaaaaaggg ctgtatctgt tttttccgtt
    13741 ttatagcgtt gattccgaat ggccagataa ttctctaaat aaggtttagc aaaagcagcg
    13801 acattgactg agtcacgttt gcaacctttt cgagtaacat caataaccat catttttaga
    13861 ttgagatctc ttagatctag attaacagct tcagataagc gaacaccaga tgccaagaga
    13921 agggcaataa tggctaaatc tcgttcttta tttttgttga atgatgagag agctcgattt
    13981 gaaagctgtt gtgggtgctc ttgatcgata taagttagaa aaccttctgt ttcatcacct
    14041 agaaagagtt tttgcttgat attttcagct ctggcagcaa gggtttcttt ctttttcttg
    14101 gtggaaactt ttttcattac attacgatag aaataaggtt ccccctgatc gttttcaacc
    14161 tcctcggtta gatacttgta aagactagaa agtgctgata aggttcgatt gatagttgtc
    14221 tgtgaaacac cttgttttgt tgtattagca ttcagcaagg gacgttcacg tagataaagg
    14281 ataaaggatt ccatgtcttt cttagacata ttttccaaaa ctgataaagg aatatcggat
    14341 attttatcag cgtttgaaat accagactcc aaaacccagc tgaaaaatcg gtcatattcc
    14401 ttaaggtatt cgtacagggt tgtaaaactg tagggcacag ccagcttaga ttggtagtat
    14461 tccagaacat accagggcat gagttgtttt agtttgtcga ttcgttccag taaaatctca
    14521 cgtttcatgt attttctcca taagtaagta tactagtagt atagcataga cttgtatatt
    14581 tttcaagaaa attatagttt ttcggaaaga tgttatagga gtttttcaaa gtgataaaaa
    14641 gacttagacc tacaaacaaa acagaataag gaaaataata aaaatctcac ccactaaact
    14701 tctgttaata ctacataact gaagagctat ataaggagct atgagataca aaacacattg
    14761 tataatagat tgaaactaga atagtacatc tggacttcta aaacattgct agaaattgat
    14821 ttgattgtcc tgattgtttt atcctatttc tatttcattt ctctatataa ttgctattgt
    14881 tttctttttc atgattttaa tcttactgta cagatttgat acagtaaact cctttctagc
    14941 tttatacttt atcatttcta acttaattat agtcttattt ttaataaagt tcaattattt
    15001 atatgtaaaa ttcctcatca gtaaaaaata accgatctca ttcctgagaa tcggttttct
    15061 aaataaatca aactgatcat ttacataaca taaattatgt aaatactcat actacaacaa
    15121 caaatctttg acttttccaa tttcactttt tggaataacc aggtgaatca tatcacccag
    15181 atacattctg gttgagccgt taactgtttg gctcttgcca ttatggactt gagttgtgat
    15241 gaggacgttg tgtggtaagt tgagttcatg aacttgtttc ccagcaattt tatcagaaac
    15301 tggtatttcg ataagtgtaa cttctccttc gctagatact tcttctggaa gcattttttc
    15361 cagcatggct tcatagactg gcgtaccttt gagcaaatcc atgataatat aagaaacaag
    15421 agtgacaaga ccaagtggca taaggttgcg aatatctcct accatctcag ttacgaggat
    15481 catagcggtt aagggagctt ttgatatggc tccaaaatag ccactcattc ctagaatgac
    15541 aaatataggg aattgctctt gactgacaag tccaagattg acacagataa caccaactaa
    15601 ggcaccaagc aaagaaccaa gagctaaaat ggggaggaaa attcctcctg gcagtccact
    15661 tccatagcta atcatactcc aaataaagcg gattaaaaag taagctaata aaacttggaa
    15721 actaaaattt tgttcagtta aagaaaggac aagctgattt ccgccaccaa tgatttgagg
    15781 taagaagatt ccgactggta tgataaggat aaaagccaag atgggataat aagccctatc
    15841 caaatggatt ttttgaccaa tcaagtcata aactcttcca acgtttaata cagctttctc
    15901 atagagaaaa cctgaaaatc caaggaaaat tcccatgacg agatatatcc aatactgatc
    15961 tagggtcatg ggaggaatgt tatctggcat atccaatact ggtgtcaaac cgaacatgag
    16021 tagagacaca aagtttgcta cgatgctggc tgctagagtt gagacccaga aaaagcgcga
    16081 aaaatggtga tagacttctt ctacaacaaa gagaagtgct gcaataggag cattaaaggc
    16141 tgcggctaaa cctgctgcag ctccactggc aatcaaggaa cgttcctcta ctggactgga
    16201 tttgagccac ttggcaattc ctttaccacc aactgctcca agttgaatgc tgggtccctc
    16261 tcgacccagc atgagtccac tggcaatagc aagaatacct agcacatatt ttttccaaag
    16321 aatgccccac cagttgaggg acatgaggcc tttcagttcg gcttcgactt gaggaattcc
    16381 tgagccttta atatcttttt ctgaccgtgt tagtttggca ctgagccaac agatgagtat
    16441 ataaaacaaa accagtacaa aaagattgcg cactaagtac ccttgatctt gataaactcc
    16501 ttgtatcagg tggaagccct tttcaattaa gaaacgaaag gatccgacga taattccaac
    16561 gatgaggccg acaatgattc ctcgaccaac ttgggatagt atagtgctgg atgcaaaggc
    16621 gaattctttc ttagaacgga ctatttccga ctgttcctcc atgttatttc ctttcaatta
    16681 actttctttt ttacctaaaa ccagtgattt aacgggttca aagctggttc ggtgaattgg
    16741 ggtaactcct agttttgtga ggccttccag atgtttagct gtgccatatc ctgcattagt
    16801 agcgaaatca tagccaggga actgctgatc gtattctttc agcaattcat cacgtgttac
    16861 cttggctact atagatgctg ctgcgataga gagggagttg gcatctcctt tgataatgga
    16921 ggtttgtgaa atgggcaagt ccagtttcat ggcatcaatc aaaaggtgct ctggttgagg
    16981 gctgagctgg gagattgctt cttgcatggc tagtttggtt gcttcataga tgttgacttg
    17041 gtcgatgacc tgattatcta tgataccaat tccaatcgac aaggcttggt cttgaacggc
    17101 ttggaaaatc tccagatgtt tctttttagg aattttcttg ctgtcgttga gacctttaat
    17161 cttacaattt ttagataaaa taacggccgc agcgactaca ggaccagcaa gaggaccacg
    17221 accaacctca tcaatacctg caattaaggt caatccttgc ttataaagtt ctttttcata
    17281 agaaagcatg gattccaagc gcaaattttc atctaattca gcttgaatgg ctcttttacg
    17341 cttgctgatt tccttttgaa ctcctgagcg attatccttt tcaagctcta aaaaaatagg
    17401 gctttctaac tccttgactg tcacaaggaa ttctttgatt tctttaatcg ttgccatcga
    17461 ggtcttccaa tgtatctaag gtatagttac cgagtttgcc atcacggact tccttcacga
    17521 agagactgta aaaacggtca tagtcatcac ggaaaccgag ggcgcgggtc atatccataa
    17581 taatcacagg cgcttcttct tcaattttca tttgtttgaa gcgttcagcc agcttttctg
    17641 gataatgttc tttgaaataa ttgataccaa aaatggtaac ctcatccata ggaagcaact
    17701 ggtctttgat agctccagtc aatgccaact taagtgcaac agtttcatcc tcaaacttag
    17761 gccagagaat ccccggtgta tccaagattt ccaggtcttt attggtttta agccattgtt
    17821 gaccttttgt gacccctggc ttgtttccaa caacagcaat ctttttacca gccaaacggt
    17881 tcatcagagt tgatttacca gcgtttggaa tcccgataat catagtacgc aaggtttcaa
    17941 tctgaatccc acgttctttc tggcgagcaa tcttatcagc catgagcttc ttggccgcat
    18001 ctgttacaac ttttacagtc acttgctctt tggagttgat agctagcgtc tggattcctt
    18061 gtgattcaaa atactgacgc cattccttgg tcattgctgg atcagccaag tcggccttgt
    18121 ttaaaatcaa gagttttggt ttatcaccaa caatcttggt caacatagga ttttgactag
    18181 atagaggcaa gcgtgcatct actaaaatcg tcacaaaatc aacaaatttt aaattctcct
    18241 gcacctgtcg acgagcttta gacatgtgac caggaaacca ttgaatagta gccattgagt
    18301 tttttccttt catagcaagg atttagatcc cctattttat tttactattg tttgaacata
    18361 gagagttttc aaatgaatga tgtccaaaca gttcttgttt tatgacatag aaatctactt
    18421 tatattatat catgccttct ttattttgtc cgatgatagc cttttaccct atcttttacc
    18481 ctatatagaa aaaagccatc aaatgatgac ttttcagagg gagcaaaaaa ctgttcccgt
    18541 catattatga accaaaagaa ctaaactacc aacagctaat aggcctaaag ctgtaagctc
    18601 agaagttgat gaacctgttg caggaagagt ttcctgctga taaataagat cataacttac
    18661 ttcattatca gtaggaattc ctgcttgaat cttggtttgt tcttcatcac ctttttaaac
    18721 tttatttcct aatagtagac actatttttt attctcaaaa attgaacttc taaaatcatt
    18781 tgtttggtca agataggctt taaagattgc tctcttgagc ttgtggactt cactatctat
    18841 gtctggattg tagttattcc aatggtagta attgtgttct gcatccttac gaacagcaac
    18901 gtccattaat ttctgtaatt catctacact gcttgtagtt ttcttggcaa atgtttgcca
    18961 aggctgtgtt gtatcattaa aagtaacttt gttcaatcta tcaaactgat cttgtcgttc
    19021 ttgatacata gctttcttaa actcactcca agtatgatat tggttattaa ataccttttc
    19081 caaaaccaag tcatctgtaa ccaaacctct tgttttaccg tagagattga ttgtcttgcc
    19141 cttttgtttg gcttcttctt cgtattggtt tgagatatat ggtaccatac catctttaaa
    19201 gcctttagca gctagtagtt cataggctat acgacgtccc atcaggtcac ctggtgttcc
    19261 tatatcatta cttaatgcag cataaatcgg tgcaaataac ttaatagtga agtagccatt
    19321 tctttcgtat ttacctgagg catattccct agacgaaaga atattattat caatcaaatc
    19381 attgaatgaa cgtagtcttt caacttcttc tactgttaga tttctgacaa catttgttgc
    19441 atagacctta tttccatcag gatcaggatg gtactcattt gtaacttttc taagaagttg
    19501 ttgtttttga ttcgtatcca atttaagaat tgaatttcct tcgagatatt ccaacatata
    19561 aacaacgtca aacatgttgt ggacatattg cttcaaatca tctgcattat taaatcttgt
    19621 agttggatca agtacttgta atcgtcgact ttctgtacta tcagattttg aatgtttcaa
    19681 gatggagttg atggtaatgg tcgcatcatc tggatggtct ggtgcttgta ataatccttt
    19741 agcaaagaac tctggtccca agccacttct tcgaccatat cctccaagat aaatgtcctg
    19801 atctgagtca tgtgtcatct catgcgtata agtaatagct ccatccttat ccaacattcg
    19861 ataacccata taataaactg catcacctgt agcataagca ccgtgttgat tatgcccaac
    19921 tttatttcca acaggtccaa agaaatgttg cattgcagga tttggattat caaaatctgc
    19981 cacttctgta gctttcccta cggtattatc atcgccaaat ttataagcat cgtaaagcaa
    20041 aatatttcta taaagttttt cacgtgcatt gtcgtctaaa atacgatacc aataatcgta
    20101 gtgatctcgc tgacgtttgg ctgtttcacg cgcattttct tcaacaaaat cattgagagc
    20161 cttgcccgct ttatggtcac tactgcggta gcgatcataa gctccaaatc ctagactaga
    20221 catggtcgag atgacaaata cggatctctc tggcaaggtc aggagaggca agaccatatt
    20281 gcggtatttc catgtggcac tcgtgatacg atcataaaca ccgatagaat acttggtgcc
    20341 agctaaccct tgcttcgttt tcacctcttc gatagtggat ttttcttcga caatgtaagc
    20401 cttagtctct gatttaaacc agtcattatt gcttgtattt ggtaaaaaga cttttcggta
    20461 atgttccagc gtgctaaaca aatctgtcgt tccatgttga ctggcaagac tgataccata
    20521 agtatcgaca ttattcttag ctagaagatt gttaaagcca gatttaccca actcaatcag
    20581 agtatctaat ggtgaagcat tccccttacc aaagaagtcc aaatggtaca gaactaggtc
    20641 tttgacattc acctgaccat agctaaagtt ataccaccgt tccagatagg tcaagccaag
    20701 tagcaaggct tccttgttgc gtttgatttt atctacaaga taaccttcag tgacggggtt
    20761 agcactagcc agtccagcat ccgctgacaa gagttttttc aaactgtctt ccagttgttg
    20821 ttttgttttg gcgaactggt cttctagata gagctcagtt tgcttgacgt ttggagaaat
    20881 acccagcgtc tttctgatgg cttctgaatg atagtcaacc ttttgtaagt caggtaagac
    20941 ttgcttgatg atagaggttt ggtcatacag gaattggttt ggcgtataga gaagtccagt
    21001 attgcccaga ctatattctg ctaatttggc gaaatcattc tggtatttga gatccagctt
    21061 ctcagataaa tcatccttgt agtgaagcaa gagtttgttt gcagtctgtt tgttagaaac
    21121 aatgtctgtg atgacttggt tgtccttcat catgactgct gacaagagtt ctttttgata
    21181 taaaagactg ttctcattga ccaggtttcc gtatttgacg atggttgcct tgttgtagaa
    21241 aggtagcaat ttttcaatgt ttttataagt caagttgcgc ttagcttgat aataggccac
    21301 cttagaaaaa tcactgtctt ttttgccact tgttgaaagt ggctccactg ttggtagaat
    21361 gagaggattg atttctgctt ttttgcttgc aatttgagaa gcatctagca ttgttcctct
    21421 ttcttcaaag gattccttgc tgacgacctc atccttgacc aaggtgacat tgtagactct
    21481 gttggccttg ctgctgaatg tgtcctttac cttcatttcg ttatagtggt aaccagtgat
    21541 ggcatttccg ttggttacat taacatcgct gagaacattg gtcaaacttc cagcatgcct
    21601 aacatcacca gaagttcgat cccacaaatt gcctgccact ccagcgactc taccaaagtg
    21661 cttgacattg ttgatatcac cttcagcata gctatcttgg atctgtgcat ctcggtctac
    21721 taggcctgca agtccaccca cagtctgatc tgaagtattt gtgttagatg aaatggctac
    21781 tgtcgctttt gacttagtaa gtaaagcctt gtcacctgtc aaatgaccga ccataccacc
    21841 gatattgtag gcagcagtcg tttcataagt gttgataatt cttcccttga aactgctctc
    21901 tgtgatgctt gattgctcag ccttagccag caaaccaccg ataccacgtt caccagccag
    21961 aacaccatcg acgtgaactt gcttaatttt tgtgttattc tgagcttcat ttgccagtga
    22021 accgatatca tctttccctg aaatagcaac attttttaga ctcagttttt ctactgtagc
    22081 accactcaag ttttcaaaca gaggtttttt caaattatag atagcataat tcttgccatc
    22141 tttttcaccg attaaacgac cagtaaaggt gtccttgata taggatcttt catcaggacc
    22201 aagctccact tcgttagcat tcaggctggc cgctaaatga taggttccag agggattttg
    22261 gtttatagct ttgaccagat tactaaagga agtaaagttt gttgtttctt ctgttccctt
    22321 cttagctaga tagaaggtaa aattatcttt atatctgctt tctatctcct gctgaagctt
    22381 ctctactttt gctgtgattt tataaaggat tttatcattt tttctttcct ctgatattga
    22441 tgctactggt aggtatacat ctttgaatga agaagatttc actttaacaa agtagctatt
    22501 tggattgctt ggaacttgct ctaacgaaat gtgttgttta taagtaccat ttgacaaact
    22561 gtataactct aggtcggaaa catttcttaa ttcaagtgtt ttctctggtt cttctacctt
    22621 tttatcaggg tctagttcat tttcttgttt aatttcttcg tttccatttg aattggatgt
    22681 gtttgattcg gttgaaacat cctcagttga atttccgttt gatggttctg gttctgtttg
    22741 tccattctct gatgttgtat tacctgaatt ttctggtttt gttgcagttc cgtttttttc
    22801 tggttgattt gattcttcaa ctggtggttt tgaatcacta ggtttattgg atacttctcc
    22861 agtattttcg ttagctattt tcccagagtt tgtttgtgtt tcttctgcag gttgaactgg
    22921 tttttctgtt tcttgatttg aggtaccttc tactgtgcct tcatttggat ttactggaac
    22981 ttcttctaca gttttttctg aattttcatt tttagagtca ttatgttctg gtttatttga
    23041 ttctccaact gaggttgtcg aatcactagg attactggac acttccccag tatttttgct
    23101 agatgtatct ggtgatactt tctctgaatt cgttgttgat tcttctgcag gttgaactgg
    23161 attttctgct tcttgaattg aggttccttc tgtagtacct tcatttggat ttactggtgt
    23221 ttcttctgtt ggttttactg gaacttcttc agttttttct ggaccttgtt ctttggtctt
    23281 ctcaaccgga gtttcaggtt ttacttgctc aatattaccc ttatattctg gaagcggtgc
    23341 tacctgctct ggttcacctt tatcacttac cacagtatct ggcgactctg gttgaacctc
    23401 agtctcacct ttgtcggtca caactgcttc gggtaatgta ggttgaactt ctggttcgcc
    23461 tttgtcactt actacagctt cgggcaactc aggctgaatt gcgggttcaa caatagctcc
    23521 agactgtacg tccttatgtt ctacaccagt ctcaggttgt tcctttataa cttgagtttt
    23581 tttagtacct ttttcgacta ttcttggact aggcgcagtc gttgaagttg aaacaatttc
    23641 tcgcgaaact tcttccttgt ttacagagaa tattctgacg atttcaactt tcttacctaa
    23701 tttaccttct tgttttactc ttacagttcc ttcagctaaa tcaggatttt cttgaatttc
    23761 ttcttgaaaa tctatttttg tctccatagt ttcctcacga tataagagtt caggtttgtt
    23821 caattgacct gataaaactt catcctgtgg atttaatgta tttaccccag tcttttcttt
    23881 tggagaaatc ttctcctctt tcttcgtttc tagattctta tgttcggcta attgttcttg
    23941 agaatctgaa gattgtttct cttcttttct tggattgatt aattcagtag agaaaggttt
    24001 ttcaactact tgaacttctg tcggcttagt tgaagaaaca ggtgtttgtt cctgaatagc
    24061 ttgtactgtt gatggatggt ctacaaaatt cggtgtaaca ttataatcca ccttttgttg
    24121 ttttgtagga gtggcaactg aactcttttg attacttact tcagactcag aagtcgtttt
    24181 tccctctttg atatatccaa tataagtgta acctgaaatc tctttaggaa gaggtaattt
    24241 ttctccagag gtcaattcat agtccgtatt gtaatttagc aaaagatgat tttctaaagc
    24301 atggactgaa actaagacac catttcctat ccctgcaacc aatactaaat gtaataccgt
    24361 tttattctta acctttttct tggaaacagc aaaaattaaa attcccatag cagctaagct
    24421 agcaccagca actagggctt gcctctcatt cttgcttcca gtatttggca attccgccag
    24481 ttgattttga gaatttaact tataaacaag ataataagtt tcatcatcat tctccacgta
    24541 tgtcggaata tcatagacaa gctgcttctt ttcttctgat gatagctctg aatctgccac
    24601 atatttatag tgaactcccg cagtttcttg agcatccaca gatgaactag ctaatacaga
    24661 cataaaaaat aaacttgaaa tcgttgcaga tacaagtcct actgataatt ttctaaatga
    24721 aaaacgctct tgtttttcac caaaatactt ttccattatt cctccttgaa ataaaattta
    24781 tatatgttac aaagaccttt attatattag tgtattatct attatctata gaaaaggcag
    24841 tataccttaa ttatactctt aatttacaaa aaagtcttaa aattgagatg cgctttcata
    24901 ctttgtttta tattatttgg aggtacaata acacctacca tgaaatttac acggtaggtg
    24961 ttactcatat cactaatcgt tctaaaaatg gtttgaggca gttgaggaga attccttcta
    25021 tccagcttcc ttgtgctgat gagcgatggt cttcctgcag gctttttttt agaaaatctc
    25081 ggacttgttc tggtgcgatt tcaaattcaa aggctttcat tttatagaaa aagtcgatga
    25141 gatgatctga caggtattca gttgaaaagg gtacttcacc actttttcta tattctaata
    25201 agagtctaga aaatcgagct ttttcttcag gaagctcacg aaaataggaa ttgaggatcc
    25261 aagtctgctt ctgttttctt tcaattggat cctgactggc aattcgttgg tctttttcca
    25321 gctctttttg gtattgtttg gccttgatag ctcgttctgc tctattttta ccaaaaagaa
    25381 ttttttccca cttgcgttct tcttgagtca gggtctctgt aaagccaaag taatcttgat
    25441 aagcacgctc tgcgggtccc atggctagaa ccagattgtc tgcatattgc ttggcgattt
    25501 tatccctctt cttgcgttct ttctctgcct ggatacggag ttcttgttcg tagtcaattt
    25561 tctccttgcc tagcttgaca aggtagagtt ggtcatccga tttcccaagt aaaaagggtt
    25621 tgatacactt ttcaaggact tcttccatcc gagccttttt ctttggttcc gccttggtcc
    25681 aacttcctcc ctgaaagact tctaggaaaa gctggtagtc tctctcaggc gcaaattgat
    25741 tgccacgatt gggtttgaaa acaccttttt cccagagcca ttttagaagt cgctcgtcaa
    25801 agttactttt attgaccttg attttttcct ttttctgagc ttttctggtt agattttcaa
    25861 cctttctgag cagtttttct tcctcttcca attgctggtc aagggacaat cgatgaaaat
    25921 gacgaacaca gtcgctacca attggaaaga ggcgttggcc tgtgacaccg ttaaagagtt
    25981 cataagcgta tttgatggca tttccacaga cacaattgct acggccgata ccgttaaaaa
    26041 taaaggaaac ttcattccat tccttggtag cttgttccca agtatccgct ttcgaagcct
    26101 gtaaaactgc atcgtgcagg gattttctaa ctggaagtgt catgaggtct cctttctaat
    26161 actcaataaa aatcaaagag caaactagaa agctagccgc aatcagctca aaacactgtt
    26221 ttgaggttgt agatagaact gacgaagtca gctcaaaaca ctgttttgag gttgtggata
    26281 gaactgacga agtcagtaac catatataca gcaaggcgaa gctgacgtgg tttgaagaga
    26341 ttttcaaaga gtataagtta tacttttaca acttgaacct cgtctttacc gagtaaaatc
    26401 aagtattttt caatattttc aatcgaatag gctcgtgata aagcctcttc gtatagagct
    26461 aactgaccac gatagcggtc tacgagttga cttggttcat catagcggtc tgtcttgtag
    26521 tcgaacagaa caattttgtt ttcgtaaagc agatagccat caaggatacc acggacaaca
    26581 aagtcttcct gactcttttg gtctcgtttg agcatggaga aaggttgctc gcgataaaga
    26641 tggtcggtat tagcaagaat ttcctgaccg agtactgtgt caaagaaagc aagaatttta
    26701 tcaagattga tcttgtctct gacagcttgg ctagtttgaa cttgtttgag tgtttctgtt
    26761 aggctagcaa gggttagttg ctggctgagg tcaattctct gcatgagttc gtgagtagca
    26821 ctaccaatct cagctccagt taccttttct ttggttgaaa aatctggcaa atcgaagctg
    26881 attttcttgc ctactgactg accttgacca gcaatctcga caccttccat atccataact
    26941 ggttcgtaga atttcttgat ttgacttggg gtttgaacac taggaagttc aatagctgcg
    27001 cggtgaagag tattataaac ttccacctcc ttcagcattt ccagagcttc tttgatggta
    27061 tctgactgac gattgtctgc ttgggagcta tcttggagag gactcttggt ttccaactct
    27121 ccgatagctt ctctggtcaa ctgatcttcg ccaataaaac gataactaaa gttgagcttg
    27181 tccttagtaa acactttact gatagcccaa agccaatctt ggaaattccg tgcttgcagt
    27241 ctagtattgc tatttagttt cccatttttg gctgctgggt attccttgga ttccagcttt
    27301 tcacgagaac ccttgccgac aagatagagc tttttctcag cccgcgtcat agcaacatac
    27361 agcaaacgca tctgctcaga atagcttgct agctgtaatt cctcttcgtt ctgcctatag
    27421 gtcagactag gaatggagag tttgatggtt ttaggatagt ggtcttctac tgcccctgtc
    27481 tccatcttgg caatatattt gacaccaaga ccattctgac gactgagaat gacttctgac
    27541 atagagtctt gcttgttgaa atcttgatcc atattgagga taaagacgta aggaaactcc
    27601 agccctttac tcttgtggat ggtcatgagc tctactgcat cttttggcgg tgcgacggcc
    27661 acgcttgcca aatcgtgctg ggcttctaag acttggtcaa tcatacgaat aaaacgcgac
    27721 aaacctttga aattgctctt ttcaaattga tcagcacgca gtgctagggc atagagattg
    27781 gcctgcctag caggaccatt cggcaaagcc ccaacatagt cataataaaa acggtcgttg
    27841 taaatcttcc aaatcaagtc atagagagag tgggttttgg catacaagcg ccaagaagct
    27901 aggatatcca tgaattgctt tagtttttca gctagagctg tgtgaatcaa gcctttttga
    27961 ctacttgcca ttttttgtgc attgaccagt ttctcataga gattttcgtg gactttatcc
    28021 tctgctttct gaagggacaa acgtgctagc tcatcctcat caaaaccaaa cattggagac
    28081 ttcataaggg caaccaaggc gtagtcttgc aggggattgt gaatgacacg aagagtgtct
    28141 agcatgactt gcacttctag ggattggaga taattgtttt gctctccgtc agttttgaca
    28201 ggaattccgt actcagacag ggcgaggaga atctggtcat tacgactgcg gctggaggtc
    28261 agaagggcaa tttccttaaa ggcaacacct ttttcttgat gaagtttcag aatctccttg
    28321 ataactaagc gcatttcgcc tgttagtttc gtttctgttt gactctcttc ttcctcacct
    28381 gtatcgtcct tgtcgtagag gagaaatgct gccttgttgt ctggattggg agtcagtttg
    28441 gtattggcaa aaacaagctg gtgcttgtta tcatagttga tttcgccgac ctcttggtcc
    28501 atgagacgtt caaagacatc attggttgct gacagcactt ctgaactact acggaaattt
    28561 tccttgagga taatgagcct gccttcttgg ggattttgcg catagcgttg gaatttctca
    28621 ttgaaaatct gcgggtctgc ctgacggaaa cgatagatgg attgcttgat atctcccacc
    28681 ataaagcgat tgtggccatt agacaacaat tccagcatcc gttcttgaat atggttggta
    28741 tcctgatact catcgaccat gacttcatgg aagcgctcct gataagactc acgaacttgt
    28801 gggaaattct ctaaaatctc aatggtgtaa tggctgatat cagcgaattc gaaggcattt
    28861 tcctgtcgtt ttctctgacg ataagcctct acaaaatcgc tcatgaaaga ttggaaggtt
    28921 ttagctagtt tccaagtgtc tccatgataa cgttcttgat agtcgagaat cgctatctgg
    28981 tctgataatt gtcctagttt agcaaactgg gtctttctct cttcgttgta ggcatcagcc
    29041 aggggcttca aatcagcctt acggctggca ttagtcagag ctcgaccgtt tttctcctta
    29101 gagatggcga caacacgcgc aagcactgcc tgataagcct gactatcgga ctcctgattt
    29161 agggagccaa tttcatccag aattaactga acattttcta aataggcagc ctttgcaaac
    29221 tccttggcat cgttatccag atggtaacgg aaaaagcttt ccaaatccca aagggcttgt
    29281 ttgatttgct cggtcagttt ttctttttca ctggtaaaat cagctttctc aaatcctttg
    29341 aggaaagatt cactcagcca cttttgagga ttactggtgg attggaggaa gtcatagatt
    29401 ttatagacct gctggcgcag accccgttcg tccttgccac gcccagcaaa gtttttcagc
    29461 aaatgactaa aggtctcttt ctgtttacct tggtaatgcg cttcaaagac ctcatgaaag
    29521 acttcgtttt cgagaataag ttgctcgctt tggttttgta aaatacggaa attaggtgca
    29581 atatcaagca gataaccatg tttgccaagg aatttttgtg tgaaagaatc catggttcca
    29641 atggcagcgt tgggtaggtc tgccaactgg cgacccaagt gttgtttgag gtcgacatca
    29701 tctgtttctt ggattttctt gctgattttt ttctctaaac gttctttaag ttcagttgca
    29761 gccttgacgg taaaggttga gataaagagt tgagaaattt cgacaccacg cgccaattgg
    29821 tccagaatgc gctctgccat gacaaaggtc tttccagaac cagccgatgc tgagaccagg
    29881 atattctggg cagaagtgta gatagcttcg atttgctcgg cagttttctt ctgttccttg
    29941 ctcgaatttg cttctgcttc ttgcagtttt tgaatctcct cctcacttaa aaagggaata
    30001 agcttcatcg attcaactcc tctcttattt tttcaagcca agcttgcttg agtttttctc
    30061 cgaccagacg cttgccatca gctaggtcca acttttctag gaaacgggct tggcccagat
    30121 ggtaattggc ttcaaagcct gtaatagcct gatgttgctg gacgtatggg gcaatgcttc
    30181 tgccattttc agtataagga ttgatggcga accggcctgc taaaatcttc tcagcagctt
    30241 tcttgtaaag ataggcattg tagtccagta ggagctgaaa ttcctcatct gtcagttgat
    30301 tagccttgtt tttgttataa aattcgccta aataactgct ttctttttcc aagaagagcc
    30361 cttggtattt catagatttg ctggcttcta ccactgctcc tgccagactt tttaccgcca
    30421 tcagagattg gacaggttca gccatttcca agtacatggc gccgaaaaag ttctgctccc
    30481 cttctctttt tagggcagca agataggttg gtaactgaga attgagccca ttaaagaaat
    30541 gaggaaactg gaactgagtc agactggatt tgtagtctac tactcctatc gctccattag
    30601 ctttcaaacg gtcaatccgg tccaccttgc ctcgtacaaa gacactgcgt ccattgtcta
    30661 attgaataaa ggcttggtct tttccaccaa aatttgcttc ttctttgatg gtttcgatgg
    30721 ctggattgtg tcggagaata tgtccagttg tccgtgcaac atcaagcaaa acttccttgg
    30781 taaactgggc ttccaaactt tcttgataaa tagcttcaaa ttcgcgttct tgactggttt
    30841 cttgaatagc ttgttctaga cgttggtcaa aggaatcttc attaggcaac tgtaaggcgc
    30901 gttcaaagat acgatgcaag aaattcccgt gactacgggc atcaggatgc aaacgtaatt
    30961 cctcctgcaa gcctaaaacg tagcgtagga aataactgta ttcattgcga taaaactctg
    31021 tcaaacccga cgtagacagg taaaactcct gtttggcagg atagagagct tgcaaggtgt
    31081 ccttggctaa ggtcttgctg cttggactgg ttgggatagc tggattttcc agaccttgct
    31141 gatctagttt tttacctatg acacgcgaca gaaccttgac aaaagtcaaa tcttgctcag
    31201 tatcgctcat ctcaccctgc tggtgatagg caaccagact agacaaaaga ctgtgatagg
    31261 accccatatc ctccttagac agtcctttgt gattcatcct cttctctctc cgcctaaatc
    31321 caaaatggat caactcttga agataggcag attccttact ttcactttcg ttaaaaaggc
    31381 ttggagccga caagaacaac tgcttacgag cagaattgac caaggaaagc atagtgtagc
    31441 gatttttctt gagattttca ctgctggcaa tcagtaattg aacgccttct tcggtcgctt
    31501 ggtttaggtt ttgcctttct tcatctgtca gaagactggt gttttgagaa atttttggta
    31561 aattgtcctg agttagtcca atagcataga caaagtcagc agtcaatggt gcaatcaaat
    31621 cgtaactctg caccagaaca gtgtccactg ttgctggaat ggtacggtat tgggacaaac
    31681 tcattccaga atggagcaag gctaggaagt cttccagact aacctgtgaa ccagcaaaaa
    31741 cagtcgcaaa ttgttctaaa acatggcaga aagccttcca aacttcggct tgtctttcct
    31801 gttctacagc ttccaaagtg gttgtcaaat cttgtaactg cttggtcaca gctccttctt
    31861 ttagaaagac actccatttt tgtaggagtt tttcagcctt ttgttttcgg ctggcaaaga
    31921 gggtttcaag aggtgctaaa attctcaggc ggaggacatt caaacgctca agattaaatt
    31981 ttccatggtg ggatttggtg aaggtttgct gaaaggctgg caagccattg ataccaagat
    32041 agcggatata ttgctcaaaa gcatcaatat cagactgact gaggtcagta tacaaatcag
    32101 ttctaagaag attaatcaaa tcctcctgac gaaaacggta acgttttaaa gctaaaatag
    32161 actcgacaaa ctgagtcaag ggatgatgag ccatggcttc gcttctacca agataaaaag
    32221 gaatctgata ctggtcaaaa atggttttga gagataactg gtaagaagct acatccccca
    32281 agagaatacg aaaatgcttg tagctcaggt ctgagttctc atgtaatttc tgacgaacac
    32341 tacgggctac tagctccaac tcctcctttt gcgtcaaaca agaccagatt tgtaaatttt
    32401 cacggtcttt ctcatcgaca tccaaagcga gttctgaaaa gtcataagaa gactccaaca
    32461 aacgagaggc cttgtcaaaa ctatccatct tctcatgagt ttgagaacag tcctgagcag
    32521 gcgtttggta tttagaagcc agatgatgga gaaattttac gctggcttgg tagagattgc
    32581 cctcgctaaa aggactggta taggctttct tactagcata agccccgata acaatctcaa
    32641 cacctttgcc gtgaagtaag tccacaaccc gctcttcctc agcagaaaaa cgagtaaagc
    32701 cgtcaatgac caaggcgatt tgattaaaat cactacttac cttgtcattc tcaatagcct
    32761 caatcaaatg ggacaactga ctttcctggg ctaactgacc ttgattaaga taggctgtta
    32821 ctttctcaaa aatcaagagt aaatccgccc tcttatcctc atctgttaaa ttctccaagt
    32881 ccaaaaaact catctgagat ttggtcatct catggtaaag ctcaattaac tgctggatca
    32941 attgaggatc ctgcttaata gcgccataaa cacgcaagtc cttgggatcg agttcggcaa
    33001 ggcatttgta aaaggccaac ccaagaccga tatcatcaag agtagtttta gctggtaaat
    33061 cattcaagac cagatagcga gccatttgag caaagcgcgt gacggtaatc gaaaaagaag
    33121 cctgctggga caagtattcc agcacggcgc gttccttttc aaaagaaaga gagttggggg
    33181 caatgtagaa gacccgcttg ccagctgcaa ctagctcttc tgcctctctt gttagaattt
    33241 ctgtcaaaga agtccgaata tcagtataaa gtaatttcat ctcagcctcg ttggaatttt
    33301 tcatcaccct atattatacc atgattagcc tcgtaaatct gttaaaatat ttaggccatc
    33361 ctttcttttc ttcatcatct gctaaatctt aaatacttag ctttacttgt attagataga
    33421 ataagtctgg ctactgaaaa tcacataata aaaaagcctc ggtaacaagg ctttgagttt
    33481 tatgattgtt tcttaggtac ggaatacact tcaatgtgtt gtcccagtat cttaatgtcg
    33541 actggtagat tgtctgattt atcgccatca acatcggact ctaattcgat atcagaagaa
    33601 gttttaatat tacgtgcctt tatatattca atattcttga tagaatgatt gaactatagt
    33661 aaattgaaac tataatagta caccgtggat gctaaaatat ttctagaaat taatttgatt
    33721 tccctaatca agctattcgt atcttatttc aatctactat a
//
LOCUS       DAVKNL010000021        32552 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVKNL010000021 DAVKNL010000000
VERSION     DAVKNL010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32552
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.021"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(186..1748)
                     /gene="guaA"
                     /locus_tag="VW411_000955"
     CDS             complement(186..1748)
                     /gene="guaA"
                     /locus_tag="VW411_000955"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEU9793710.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            1890..2588
                     /locus_tag="VW411_000956"
     CDS             1890..2588
                     /locus_tag="VW411_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9793711.1"
                     /translation="MLPAYIKIHDQIKKDIDEYRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(2631..3527)
                     /locus_tag="VW411_000957"
     CDS             complement(2631..3527)
                     /locus_tag="VW411_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU9793712.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAIFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(3536..4471)
                     /locus_tag="VW411_000958"
     CDS             complement(3536..4471)
                     /locus_tag="VW411_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEU9793713.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(4468..5193)
                     /locus_tag="VW411_000959"
     CDS             complement(4468..5193)
                     /locus_tag="VW411_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEU9793714.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDIASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(5277..5912)
                     /locus_tag="VW411_000960"
     CDS             complement(5277..5912)
                     /locus_tag="VW411_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEU9793715.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEREEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKSLKDYLY"
     gene            complement(5914..6741)
                     /locus_tag="VW411_000961"
     CDS             complement(5914..6741)
                     /locus_tag="VW411_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9793716.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALTHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(6763..7689)
                     /locus_tag="VW411_000962"
                     /pseudo
     CDS             complement(6763..7689)
                     /locus_tag="VW411_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809127.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
     gene            8350..8961
                     /locus_tag="VW411_000963"
     CDS             8350..8961
                     /locus_tag="VW411_000963"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU9793717.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            9006..9734
                     /gene="rlmB"
                     /locus_tag="VW411_000964"
     CDS             9006..9734
                     /gene="rlmB"
                     /locus_tag="VW411_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEU9793718.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEITEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRTVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(9773..10684)
                     /gene="trxB"
                     /locus_tag="VW411_000965"
     CDS             complement(9773..10684)
                     /gene="trxB"
                     /locus_tag="VW411_000965"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEU9793719.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDNHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(10750..10974)
                     /locus_tag="VW411_000966"
     CDS             complement(10750..10974)
                     /locus_tag="VW411_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEU9793720.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(11052..11795)
                     /locus_tag="VW411_000967"
     CDS             complement(11052..11795)
                     /locus_tag="VW411_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9793721.1"
                     /translation="MIKISNLSKSFSGQTVLDHLDLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(11795..12595)
                     /locus_tag="VW411_000968"
     CDS             complement(11795..12595)
                     /locus_tag="VW411_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9793722.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(12753..13109)
                     /locus_tag="VW411_000969"
     CDS             complement(12753..13109)
                     /locus_tag="VW411_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEU9793723.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(13103..13633)
                     /locus_tag="VW411_000970"
     CDS             complement(13103..13633)
                     /locus_tag="VW411_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU9793724.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDRSNRRRSTC"
     gene            complement(13633..14127)
                     /locus_tag="VW411_000971"
     CDS             complement(13633..14127)
                     /locus_tag="VW411_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9793725.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKMPVDGERLAYQ
                     KLKK"
     gene            14262..14708
                     /locus_tag="VW411_000972"
     CDS             14262..14708
                     /locus_tag="VW411_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEU9793726.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            14705..15352
                     /locus_tag="VW411_000973"
     CDS             14705..15352
                     /locus_tag="VW411_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEU9793727.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(15373..15954)
                     /gene="pdxT"
                     /locus_tag="VW411_000974"
     CDS             complement(15373..15954)
                     /gene="pdxT"
                     /locus_tag="VW411_000974"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEU9793728.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(15955..16830)
                     /gene="pdxS"
                     /locus_tag="VW411_000975"
     CDS             complement(15955..16830)
                     /gene="pdxS"
                     /locus_tag="VW411_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEU9793729.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(16982..18361)
                     /locus_tag="VW411_000976"
     CDS             complement(16982..18361)
                     /locus_tag="VW411_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU9793730.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALAGGKIELFRNGAF
                     LVDKKQETSIPDVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            18655..19578
                     /locus_tag="VW411_000977"
     CDS             18655..19578
                     /locus_tag="VW411_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEU9793731.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            19638..20243
                     /locus_tag="VW411_000978"
     CDS             19638..20243
                     /locus_tag="VW411_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9793732.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFTDKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            20262..21506
                     /locus_tag="VW411_000979"
     CDS             20262..21506
                     /locus_tag="VW411_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9793733.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AFTGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(21729..21986)
                     /locus_tag="VW411_000980"
     CDS             complement(21729..21986)
                     /locus_tag="VW411_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEU9793734.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(22028..24064)
                     /locus_tag="VW411_000981"
     CDS             complement(22028..24064)
                     /locus_tag="VW411_000981"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEU9793735.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKGKRLSFEAI
                     QTFSTPRRLAVRVTGLADKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEIGQAVEAIVPGIVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGQETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKASLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLILSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(24276..25193)
                     /gene="glyQ"
                     /locus_tag="VW411_000982"
     CDS             complement(24276..25193)
                     /gene="glyQ"
                     /locus_tag="VW411_000982"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEU9793736.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQDCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRAKLLAEDAE"
     gene            complement(25564..26324)
                     /locus_tag="VW411_000983"
                     /pseudo
     CDS             complement(25564..26324)
                     /locus_tag="VW411_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
     gene            complement(26597..27439)
                     /locus_tag="VW411_000984"
     CDS             complement(26597..27439)
                     /locus_tag="VW411_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU9793737.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQDQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     VAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTIAVQSGAPRVD
                     DVDF"
     gene            complement(27553..28944)
                     /locus_tag="VW411_000985"
     CDS             complement(27553..28944)
                     /locus_tag="VW411_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU9793738.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKHQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQYQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(29179..29556)
                     /locus_tag="VW411_000986"
                     /pseudo
     CDS             complement(29179..29556)
                     /locus_tag="VW411_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
     gene            29771..30748
                     /locus_tag="VW411_000987"
     CDS             29771..30748
                     /locus_tag="VW411_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU9793739.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVSQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFGKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            30745..31827
                     /locus_tag="VW411_000988"
     CDS             30745..31827
                     /locus_tag="VW411_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9793740.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(31891..32444)
                     /locus_tag="VW411_000989"
                     /pseudo
     CDS             complement(31891..32444)
                     /locus_tag="VW411_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(32478..>32552)
                     /locus_tag="VW411_000990"
     CDS             complement(32478..>32552)
                     /locus_tag="VW411_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU9793741.1"
                     /translation="YNNKKIKIKLKGLSPVQYRTKSFG"
BASE COUNT         9640 a         7300 c         5854 g         9758 t
ORIGIN      
        1 attctcatta tatatatcaa aagaccgcag cttgaaaact acggtctaat tttttgtcca
       61 aatgtaaacc acttggaact attattcaat ttattctttt tagcaaatgt tcccgtttta
      121 atcccagtta cagtttttaa tcctctgtaa ccgttgattt tacaaagctt ttaatttttt
      181 tgcgattatt cccactcaac tgttgcaggt ggtttacttg taatatcgta gacgatacgg
      241 ttaacatgat ccacttcatt tacgatacgt actgagattt tttgaagtac ttcccatgga
      301 attttggcaa aatcagcagt cataccatcg atagaagtga tagcacggat tgcaatcgtg
      361 tagtcatacg tacgaccgtc acccataaca ccgactgaac gaacgcctgt gttaacagtg
      421 aagtattgcc aaatatcgcg gtcaagtcca gctttagcga tttcttcacg aagaatagcg
      481 tctgattcac gaacggtttc aagtttctct tcagtgattt cacccatgac acggatagca
      541 agtcctggtc ctgggaatgg ttggcgccat acgatatggt ctggcatacc aagctctgta
      601 ccaagagcac gaacttcatc cttgtaaaga gtattgagtg gttcaatcaa ttcaaactgc
      661 atatcttctg gaagaccacc cacgttgtgg tgtgacttga tagtttgagc tgtatccgta
      721 ccagactcga taacatctgt atataaagta ccttgagcaa ggaatttcac atctttgagc
      781 ttgcttgctt cgtcatcgaa tacatagaca aactcgttac cgatgatttt acgtttttgt
      841 tcagggtcag aaacgccagc aagtttgtca aggaaacgtt tagcagcgtc tgctttgacg
      901 atattcaaac caaacttacc accgagcatg tccataactt gatcagcttc gcctttacga
      961 agaagaccgt ggtctacgaa gatacagatc aattgatcgc caatcgcttt ttggagaaga
     1021 accccaacga cagatgagtc aacaccacct gatagaccaa gaaggacacg tttatcaccg
     1081 acggtttcac gaattttttt gatctgcatg tcaatgaaat tatccattga ccagtcacct
     1141 ttagccttac aaatgttaag ggcaaagtta cgaaggatat catttccgta tacagaatga
     1201 cgaacttctg ggtggaattg gataccgtaa atgtgtttat ctgggttttc gatggctgcg
     1261 tatgggcagt cagctgatgt acctgtacga acaaagtcag caggaatctc agtaaccgca
     1321 tcaccatggc tcatcaaaac agtctgttca tcaggtgttg attcaaaaag cgctgatggt
     1381 gtgtgagtta gggttgattg accgtattca cgatttccag catcacctgc aggaacaact
     1441 tttcctccaa gtttatgggt caataactgc ataccataac agattcccaa aattggaatt
     1501 ccgagttcga agatttctgg gtcaatatca aatgaaccat cttcatatac agaatttgga
     1561 ccacctgata gaataattcc tacaggattg acttcacgaa cttcagcagc tgaaatttta
     1621 tggcttttta gttctgaaaa aacaccaatc tcacggatac ggcgtgaaat cagctggttg
     1681 tactggctac catagtccaa tacgatgatt ttttctacat cttgcaaatc agttgaaatg
     1741 ttgctcatct ttttcctttc tcaaaagaaa tatcttttct caatattcta tcacaaataa
     1801 gggcgaatta ccaactaaac aaggcgaagt ttgacttttt ctagtttatt ggggtacaat
     1861 agagaaaaga tagaaatgaa gtgaaaaata tgctacctgc ttatataaaa atccatgatc
     1921 agataaaaaa ggatattgac gagtaccgtt gggctattgg tgagaggctt cccagtgaaa
     1981 gagatttagc tgagcagttt gcggtcagtc gcatgaccct ccgccaagcc gtatctctat
     2041 tagtcgaaga aggcgtctta gagcgccgtg taggaagcgg cacctttgtt tccagtactc
     2101 gagtacaaga aaagatgcga gggacaacca gttttactga aattgtcaaa tcccaaggta
     2161 aagttccctc tagccagctc atttcctaca aaaaaaccat tcccaatgag caagaagttg
     2221 ccaagctagg aatttttcca acggacaata ttatccgaat ggagcgggtc cgctatgccg
     2281 accaagttcc cctagtttat gaagttgctt ctattcctga aaaattcatt aaggacttta
     2341 aaaaagaaga aatcaccagt catttcttcc aaaccttgca aaaacatggc tatcgtatcg
     2401 gcaaatctca acagaccatc tatgctcgcc ttgctaaaga aaagattgcc cactatttgg
     2461 aagttgaaaa aggacatgct attcttggat tgacccaggt ttcctaccta gaagatggta
     2521 ctgcttttga atacgtaaaa agtcaatatg taggcgaacg ctttgaattt tatcttgaaa
     2581 ataattagat taccacgcaa aaactctctg tcacggacaa agagtctttt ttatttttct
     2641 aagaataaat ctttcaaaga aatcaaagtt acagcaaaca atatcattgc cataccaaga
     2701 aaatcaatgg gataaaattg ttcattcatt attaagaagg caaagaaaat cgccgatatt
     2761 ggctcaattg aagccaacaa gcttgacttg accggtccta tcagactggc tcctttaagg
     2821 aaagctgtat aggcaaagac agtcccgata aggataatgc ctgcaaacgc aaggagaaaa
     2881 tcaagactag tcgggatatc ggcctgtaga acccctgtaa aaggaagggc gaccaaacct
     2941 gctatgacca ttcccacacc aatgaccaag ctgctccccc actttttaat caaggctatg
     3001 ggtaaaatga tatacagagc ataagtcaag gcagaaaaga gaccccagaa cagaccagca
     3061 ggtgtcatgg ataactggtc caactgccca tgtgttgcga tcaggaaggt tcctccgatg
     3121 gcgaatatga tggaaactat ctctcccagt gtcggtgcca ccctatcctt gatacagcta
     3181 taaattaaaa ttccgacagg acaaacatac tgaagcaccg tcgctgttcc cgcattggtc
     3241 tcctgaatag cagacagata ggcgaattgg ttgagaaaaa gaccaatcag agcaaaaata
     3301 agaagagaca gcaaactctt tctatccttt aaaaagacca gtattttatc ctttgcagta
     3361 gcataagcca agagcatgag aattccacca gcgattaaaa gacgcaagtt agtcaagacc
     3421 agagccgaaa ttccgtgtgc cattaggtat tggccactcg ttcctgacaa cccccaagca
     3481 ataccagcca caactgttag taaagtccct tttaaactat ttgacatgtc tctccttact
     3541 cttctttgcg aatcaagttc atcacttgat tgcggtctaa tatccattga gctctctcgt
     3601 ccttttcata ggtacgattc gataggaaaa tagtggcttc ttgcttctga cgattccaca
     3661 tgataaaggt acctgtatag cccgtatggt ctagccaatc tccttccaaa ttccatgcta
     3721 aagaacgttc cttgtcatcc aaaggagaaa aattttgatt taagtctctt gcaaaatcat
     3781 ctgctaaata gtgttctaaa aagatttgta aatcctttat agtcgaaaat aaaccagcac
     3841 tcccagcatg tctacccagg agacgagcct tgggatcatg cactatgcct gcctctacac
     3901 ctctaactgt tggaacagca agctcaactg gcccaaactt agtttccgtc attccccaag
     3961 gtttccagac ttgatccttt aaaatcacat ccaaatcttg attaaaaatt ctttccaaaa
     4021 taaagcccaa cagcaaaaaa tggacatccg aataaagaaa ggctggctga cttcgtctgt
     4081 tgagatgaaa catcgcttcc tttaattcag gggctgttaa aagatcacga ttaggaataa
     4141 aaggatcaag gtctgttgca tgagtcaaga gctggcgaat agtgatgtct ggataatcac
     4201 tctcaggtaa aaaatctatt accagtctat caatatctaa ttgacctatt tcccacaaga
     4261 aggtacaaac tgtgccaacc ccaacaacct tgctgacact agctaggtca taaactagtc
     4321 ctgcctcagt ctgcaagcca tgctctgggt cactctggcc taaatagaac tctgtccatt
     4381 gattgtcctt aaaatacgca aaagaggctc cgggataaat ccctgcctcg atttgttctt
     4441 ctattttttt aataatcttg gtccacttca tacttcttca aaccacaatt caacattatt
     4501 tctatcttta ccaaggaaga atttttcaga cttaggaata aaatattcag tagactcaaa
     4561 cttctggcga agacttgcta tgtctaattc attgaccaag aacttgagca tagataagtc
     4621 ccaggtaacc gtattgtcca cagtcaaatc ctgcccctga gctggaataa aatcaaggga
     4681 tgccccaatt tcagatgatt ccaagaaact ttcgatatca gttgggagat gtaattccat
     4741 agaaatctca aatttactta aagaaattga tgccaaatct gtttgaaatt caggcttttc
     4801 tcctacttct actagacttg ctatgtcatc ttccgcatga atcaaaatca aatcatcttc
     4861 tggtgagaaa atttcaaaag cgtagccatt ttgaccttta tataatcgat gaatcgaatc
     4921 tgttttagat aagattcctt caatttctaa gggattttcc accttgacaa tcaatctagc
     4981 tagttttttt cttccctcta ccttacgagt acgcatactg ggagcttctt ctaaaaccag
     5041 cttttcaaga cccgtttggt cacctagtga cagaaaggcc gattcttcta acaaggcctt
     5101 cattccaagg gtttcaatat aaaatgtttc atttaatttt ctattattag cttttaaagt
     5161 aggaataatc cgtacaatct gatttacatt cataaattcc tccgtcactt tttattttaa
     5221 aggattttag aattttttac aagatattat gctcaaattt aacattattt agctatttag
     5281 taaagatagt ctttcaagga ttttgacaaa gcctgataac cagcaatact gaggtgcagt
     5341 ccatcagttg tatattcttt tttgagttgg cctgcttggt ctgtcaaaca atcaaatact
     5401 ggcacaaatt ccacctgcat ataggcagat gcaagctctt gataggcttg attccagttc
     5461 tgaatttttt cattcgagcg gatatagact gcctgctggt actcctctct ctcattgaca
     5521 ggcaaaatgg aaagcaattt aatctctgtc aatggataat cgcgagcaac ggattgaatg
     5581 atagcttcga gattattgag agcctcattc acaggaacat cctttccgat atcatttgtc
     5641 ccaatcagaa gaaaaatttt atctactgct ccaccatata gatgagcatc aaggttctct
     5701 agtaacagtc ctgtctgata gccacgaatt cctcgattga caatcgtctt tgaagtccca
     5761 aatagctcct gtagaggata atactcgaca atggaatccc caataaaaag aatgtttggt
     5821 tctacaacag aaatgtgatt taggtgacga tacttagttt gaattttttc ttgttccttt
     5881 aggagccaat tttctaataa ctgtactgcc acttcattct cctttttcta accagttttc
     5941 caagacttga taaactcctc cttggctgtt ggctggtgct agagcagtcg ccacagcttt
     6001 ggctttctca tcagcatttt ccatcgcata ggcaattcca gccatttcaa gcatttcaac
     6061 atcattttca ctatcaccaa aagccatgat ttcttgggat ttcaagtccc agcgcttgag
     6121 taattcctcc aagccccatg ctttatgaat cccagcttgg aggatatcaa tgcaaccata
     6181 gccactggat acagctcgga cacggccatc aaagagagca ttgatttctt ccaaaaccga
     6241 actcaaacgt tcctcaccaa caaccatgct catcttgagc acaccaccaa agaggtcaga
     6301 tgttaattca tccacaaatt gcatccgttg gtagaatttt tcaatcattt ctggagtcat
     6361 aaaactttca agatctgtaa aaatcgtacc ttccttgaca aaatcaccct tcatccccgt
     6421 tacaacaaac tggtcctgac acgctcgacc cttgaaatga gtcaaagcct tgttgacaat
     6481 ggcgtcatcc catgtctgag cctgaatcaa ttcattgttt tcaaaaatac gagcgccatt
     6541 agcaacaacc agaaccactc gatccaccaa gggactcagt agttgtctca tgcggtgaat
     6601 ttcattgccc gtcgcaatga caaaacgaat gcccctttga tccaactgat ctaaaatctt
     6661 ttccaatcgt gggagatcaa gctgacctct agcatccagc aaggtcccat ccatatctgt
     6721 cgcaattacc ttaatcatca tttactttcc tctggattca agctaatacc acttcaatcc
     6781 cacatgttca ttcatttctt tataggctgt atcaattcgt tccttagtcg cttcatctaa
     6841 ctggtcacga tgactgccat cctctataaa atcttccaaa atataagttt ggtaatgata
     6901 caagggatat ccttctggtg aaaaggttcc ctttggtgtt cgattgaccc aagtaggatg
     6961 agctttagct gttccgatag ttgtttttcc atccttcttc ttgatggtga catccatgag
     7021 aacaccacgt tcagtccact tagcattctc ttcatctccc atagattcaa ttcgttgatt
     7081 ggaaatgaag ttccccattt aatagataat gagtttctta tctccatctt tttcaaccgt
     7141 ttcagatggt tcaacaacgt gaggatgccc tccaaagata atatccgctc ccaaatcgat
     7201 catcttgtga taaagagctt tttgttcttc agttggttcc aatcgatact caacacccat
     7261 ctgaagcatg ataatggtga tatctgcttc cttctctgcc cgttcaattt caaccttcat
     7321 cttatcttcg tttaaatctg aaagataacg attatagtct tcctgagaaa tatactgctc
     7381 aattccattg aaaccatagg aataggctaa caatgcaacc ttgataccat tcacttcctt
     7441 aatgaccagc ggagcctgat cacgtggttc gtgcgtataa actccgattg gagtgattcc
     7501 agctttctca ataatatcgg ccgttgaaat aactccctca atttgcgaat ccaaaatatg
     7561 attatgagct aaatccagca catgataacc tgcctcctta atagcatcca taacttcaac
     7621 aggagcatta aagagaagat aacccgctaa ataatgatcc ttattaatgg ttccttcaaa
     7681 atcaccaata gctaaatctg ctgcttagtc cccttgcttg agccaaggag tcacatactc
     7741 aaaattttca tgaaagtcat aggtaccgtc ttctttttta gctgaaaaga aaagtccatc
     7801 gtggtagagc aaatccccgt gggccataat tctggcagtt ttttcctctt cctactcctg
     7861 agacttttgc ttagtcccct cttgagaaat agtatctcgt ttttgactag tcaagggatt
     7921 ccttggaagc tttcaaacaa caagaccaag cccattgata aaccaactgc tagcaagagt
     7981 atcgccacaa atcccttatt gctccacttg cgataactcc taaaaagttt accaagccct
     8041 tcataaaacg aaaagctaaa ccaccctgat ttcgattttg tcttctttgt atcttcgttc
     8101 tccctacttt cttatgcaag ccttttcttt ttattatatc acagataagt atttctttca
     8161 caattgaatt gaacttccta tctattttct ataaatccta aatgccataa tgctttcaat
     8221 tcttgtcatt ttgtgatatc atgtagaggt aatgaaagaa aatgaactaa tccacagtgg
     8281 cttattccaa gtataccact tgggctttgg cagtagctaa ctgcactaaa cataatataa
     8341 ggagagaaaa tgtctgcaat agaacgtatt acaaaagctg ctcacttaat tgatatgaac
     8401 gatattatcc gtgaagggaa tcctactcta cgcgcgattg ctgaggaagt cactttcccc
     8461 ctatctgacc aggaaatcat cctaggcgaa aagatgatgc aattccttaa acattcccaa
     8521 gatcctgtca tggctgaaaa aatgggactc cgcggtggtg ttggactggc tgctccccag
     8581 ttagatatct caaaacgcat tatcgctgtt ttggtaccta atattgttga agaaggcgaa
     8641 actccacagg aagcctacga tttggaagcc attatgtaca atccaaaaat cgtctctcac
     8701 tctgttcaag atgctgctct tggcgaagga gaaggttgcc tgtctgttga ccgtaacgtg
     8761 cctggctatg ttgttcgcca tgcccgcgtt actgttgact actttgacaa agatggagaa
     8821 aaacaccgta tcaaactcaa aggctacaac tccattgttg ttcagcatga aattgaccac
     8881 attaacggta tcatgtttta cgatcgcatc aatgaaaaag acccatttgc agttaaagat
     8941 ggtttactga ttcttgaata aagaaaatcc cgttgcaaga cggggttttg tgttataata
     9001 gaggcatgaa aacaaatgat attgtctatg gtgtccacgc cgttaccgaa gccctccttg
     9061 caaatacagg aaacaaactc tacctccaag aagatctccg aggtaagaat gttgagaaag
     9121 tcaaggaact agctacagaa aagaaggtgt ccatttcttg gacatcaaaa aaatctctct
     9181 ctgagattac tgaaggtgct gttcatcaag gttttgttct acgagtgtct gaatttgcct
     9241 atagcgagct agattacatc cttgcaaaaa cacgccaaga agaaaatcca cttctattga
     9301 ttctagatgg tctaaccgat ccccataatc tgggttctat cttgcgaaca gccgatgcga
     9361 ccaatgtttc aggtgtcatc attcccaagc accgtactgt cggagtaact cctgtcgttg
     9421 ccaaaacagc cacaggtgct attgaacacg ttccaattgc ccgagtgacc aacctcagtc
     9481 aaaccttaga taaacttaag gatgaaggtt tctggacctt tggaacggat atgaacggta
     9541 ctccttgcca caagtggaat acaaaaggga aaatcgccct catcattgga aatgaaggaa
     9601 aaggtatctc tagcaacatc aaaaaacagg tcgatgaaat gattaccatt ccgatgaatg
     9661 gacatgttca aagccttaat gccagtgttg ctgcggccat tctcatgtac gaagttttcc
     9721 gaaatagact ataaaaaagt ttccagtcat ctgattggaa acttttttat gattaactat
     9781 gttctgtaat gaatttatag gcttcttgac cagcgatagc tccatctcca accgctgttg
     9841 ttacttggcg aaggtctttc aagcgaacat ctccaactgc aaagataccg tcgactgcag
     9901 ttttcatgtg gttatctgtc acaatccatc ctgcctgatc ttggatattc aattctttaa
     9961 caaaatcgct aagagggtcc aaaccaacat agataaagac accaccgaag gcttgttctg
    10021 tcacttgacc tgttttcaca ttttcaaata cgactgattc tactcggttt tcacccttga
    10081 tttcccttac tacagaatcc cagataaagc tgattttttc attcgcaaag gcgcgatctt
    10141 gtaaaacctt ttgggcacga agttggtcac gacggtgaac aatggtaaca gtcttagcaa
    10201 aacgagtcaa gaagagggct tcttcaacag ctgaatctcc accaccaact accaataaat
    10261 cttggtcacg gaagaaagca ccatcacaca cagcacagta agaaacacca cgactgttca
    10321 gttcttcttc tccaggcact cccaaaggac ggtgtttaga accagttgct acgataactg
    10381 tacgtgtttc atatgtttgg tcatcagtca tcactttctt aaaatcacca tggtcttcga
    10441 cattttcaac ataaccataa atgtgctcaa caccaagatt ttcaagtggt tcaaacatct
    10501 tttcagccaa ttcaggtcca ctaatattag cgtatcctgg gtaattttcg atatcagatg
    10561 tattattcat ctgaccacct ggcagaccac cttcaatcaa agctactttt agattgcttc
    10621 gagcagcata caaggccgca gtcatccctg caggtccagc accgataata atagtatcgt
    10681 acatatagat tccttctttc ttgttgtaac tatctttatt ctaactcttt cttgtcaatc
    10741 acgcaaggat tatgccttga aaacaagaat taaactcata accgcaaagg ataatactgg
    10801 gacaaccaag actccaatca taatcatatc atagagatgg gcttggcgag ccctggctgt
    10861 cttatcaact cccgacatgg ctctcagacc aatccaaatc cctaaaaaaa tcagaacaag
    10921 gatggtggtc aagatcaaac tctcgaaata taaagaaaat agttgcagta gcatgatttc
    10981 tctcatttct atctttttta aagagtaaac tcagctagtc caactaactg agttttcctt
    11041 tatctattat atcaaatata agtccgtttg taactagcga agaattcttt tgtccgctct
    11101 tctttagggg tgtggataat ctcatccgga gttccagact cgatgatttt ccccttatct
    11161 aagaagagaa ttttatccgc aacttgggct acaaaggaca tgtcatgact gaccaaaatc
    11221 atggtctgac ctgacttagc agcatctgca atagactttt ctacttcacc gaccaattct
    11281 gggtcaaggg ctgaagttgg ttcgtctaag agcaaaacat ctggtttcat agcaagcgca
    11341 cgcgctaggg caacccgttg cttctgtcca cctgataaat ggcgaggata atggttttca
    11401 cggtccgaaa gcccaacctt agccaactct tccttggcaa tcttagtcgc ttcttggtca
    11461 gataatttct tgacaacaac caagccttct ttcacattat caagtgctgt tcggcgttca
    11521 aacaaattaa actgttggaa aaccatagac aacttacgac gtagggcaag gatttcttct
    11581 tgagtgattt tagaaaaatc aactgaaaaa ccatcaatct gaatagagcc actgtcaggt
    11641 gtttcaagat aattgagact gcgaagaaag gttgattttc cagctcctga agaaccaatc
    11701 aaggctacaa cttccccttt ttgaatatcc aagtccagat gatccaagac agtctgtcct
    11761 gaaaaggatt tgcttaaatt cgaaatctta atcattaacg aaggtctcct ttcacatctg
    11821 tttgcactgt atcaggtgca gaaatagcca tttttctctc gatgaaacga ccgaggcttt
    11881 caattccgat attgactacc caataaacaa gggcaacgga gatgaagcgt tcaaaatagc
    11941 gataatcagc tccacctaga atctgagctt gggcaaagac ttccacaaca cccgcactaa
    12001 aagctagaga tgttcccttg gtcaaaccga tgagggaatt aatcaaggtt ggagtagcta
    12061 ccaccgctgc attaggaata atcactcgtc gataaacttg cgctcgggtc atacccagac
    12121 tgcgtgccgc ctcaatctca ccaggattaa ctgagagaat ggctgcacga atggtttcac
    12181 tagcataagc tgcctcatta aaggcaaagg cgacaatcgc aaaagctgca gctggaatcg
    12241 cattgatatt gagaccagtt ccccattgct gattgagggc tttcaaagcc aaagggattc
    12301 cgtagtaggt caacatgagt tgcaccaaaa tcggtgtccc ttttaagaaa ctaacaaaga
    12361 aggcctgcaa gggatataaa atcttgacac gattgatctt cacaatggca aaaagaagcg
    12421 ccaaaaccaa gccaaaaagg gcaccaccaa ttgtcaacat aattgttgtt ggaagttgtt
    12481 ggacaattct agggattcca tcaaagaccg aacgtaggct aaacagcttg ccatccggaa
    12541 tcaattgcat caagttttgg taccaatctg atgctaaaat cgttgtaaca ttcataaaaa
    12601 catcctcttc tgataatgat tcattctacc atgcttctgc cgtcaatgaa attatttgct
    12661 acggtcagat acagactttg tctacctact agtttatcat actttgaaac agggaagtta
    12721 tatattttat ctatcaaaga gatagataaa aactatttca gtcccagttc ctcataagat
    12781 tttcgataac cgatttgctt aacagttcca ttttctacta aaatgggccg ttttaacaac
    12841 ataccgtcac ttgctagcaa ctcagccgct tcttggtttg acaaacttcc taccttatct
    12901 tttagcccta attcacggta tttgatacca ctggtgttga aaaattgctt caattcaaat
    12961 cctgaggttt ctagccaatt caaaatgact tcttggctag gtgtttcttc cacgatatgg
    13021 acggctttat agtccacacc taattgattt aattcttgtt ttgctttttt acaagttgaa
    13081 catttggggt attcgataaa ttctaacatg tgcttctcct tctattgctt ctatctttaa
    13141 aatctactcc ttcatgctcc aagagccaag ctttcttttc cactcctgca gcataacctg
    13201 tcagacgctt gcctgctccc aacacacgat gacaaggtac taggatagac caaggattgc
    13261 gtcccactgc tccaccaatt gcttgagcag aagccacttg caggtcttga gcaatttgtc
    13321 cataggtcac tgtctgacca taaggaatgc cctgtaaata gtcccaaact ctcttttcaa
    13381 aattcgttcc gattggcgcc aagagcaagt tggataaatc ctgaggcttg cctttaaagt
    13441 aatcatctaa gcaagcaata actgggtcta aaataggatg actaacaact tcttctatcg
    13501 tttcatctcc tagtcccctc tcaaaatgct tctgctcctg aacccaaatt ccatacaaat
    13561 aatggtcatc agctacaagt gatagaatac caattggtga ggagtagagg atttttacat
    13621 acttcttttg cattatttct ttaatttttg ataggccaag cgttcgccat ctactggcat
    13681 cttaccgact tgtttaaaac caagtttttc aaaaatatgt tgcataacct tgttttcagc
    13741 atgcgtatct gagcgaaaat caagataatc aaaaccttca atcaagccct ctaagaaggt
    13801 ttgagcaact ccttttccct gcacatctgc tgccacagca atacggtgaa agactagata
    13861 ctctgactct ccagcttgcc agtttccctc ataaatagct tcataggcct cctctggact
    13921 cttggtcaca gcagcatagg ctagcagttc tccctcttcc aaggctacgt aggcttgccc
    13981 tgagatgata tcatcgataa taacatctgc atttggatag ccattttgcc actggtcact
    14041 accagcatct gctaaacatt ttttggcatc ctccatcacc tgcatgatgg catctacttc
    14101 atttggaaaa gctaaacgaa tttccatctt ttttcctcat ttctgactag tttctcccat
    14161 catagcataa tttaagtatt tctacaagca gttaaaactt gactttgtgt ttttgttatt
    14221 ttataatcta gttatcaaaa ctatataaaa aggagttaaa aatgaaaact accttttcct
    14281 acccaaaatg ggcagaaatt ccaaacattg acctctatct ggaccaggtt ttgctctatg
    14341 tcaatcaggt ctgcgcccct atctctccta ataaagacaa gggcctaaca gcatctatgg
    14401 tcaacaatta tgtgaaaaat ggttacctga caaagcctga caaaaaaaaa taccaacgcc
    14461 aacagattgc ccgtttgatt gctatcacaa ccctcaagtc tgtattttct attcaagaaa
    14521 tagcccagac acttaatact ctacaaactc aagcaagttc agaccaactc tacgatgctt
    14581 ttgtggacta catgaaccaa ggaattgacc cagctaaccc tattatccaa accagctgcc
    14641 aaaccgttaa actctatcat caaactctag acttaatcga tcatactcaa gaggaggtaa
    14701 tccaatgaac actagtctta aactcagcaa acaactcagt tttggagagg agattgctaa
    14761 tagcgtgacc catgctgtgg gtgcagtcat catgcttatc ttgctgccta tttcatccat
    14821 ctatagttat gaagcacacg gatttttatc ctctatcggc gtttccattt tcgtcatcag
    14881 tctctttctc atgttcctat catccaccat ttatcactct atggcctatg gttcgaccca
    14941 caaatatgtt ttgcgaatca ttgaccattc tatgatttac gttgccattg ccggctcata
    15001 cacgcccgtt gtcttgacct tgatgaataa ctggtttggc tatctgatta ttgtcatcca
    15061 atggggaacg accatctttg gtattctcta taaaatcttt gctaaaaagg tcaatgagaa
    15121 atttagcctt gctctttacc tgattatggg ctggttggtt ctggctatca ttcctgccat
    15181 tatcagtcaa acgacacccg ttttctggag tctcatggta actggcggac tctgttatac
    15241 agttggagct ggattttatg ccaagaaaaa accttatttc cacatgattt ggcatctctt
    15301 tatcctagct gcgtccgcac ttcaatacat cgctattgtt tattacatgt aaaaaagttg
    15361 agaaattcaa tctcaacttt tttctttaca catattgata aagtactggt gcaagcgcac
    15421 atcatcagtc aattctggat gaaaagaact taccaacata tttttttctt gggctgcaac
    15481 aatttgattg ttcactgttg ctaaaatttc tacaccctca ccaacactac tgataatcgg
    15541 accacggata aaggtcattg gaatcttgcc aactccctta cattctgctt ccgtgtagaa
    15601 acttcctaat tggcgcccat aagcattacg ctcgaccacc atatccatag ttcctagatg
    15661 actctctttc tgagaagtga tttccttagc cagcaaaatt aagcccgcac aggtcccaaa
    15721 cactggtaag ccagatagaa tggcttctcg gatgggaagt agcatgttct ggtcacgtaa
    15781 gagcttgccc atggttgtag actcaccacc aggcaaaatc aaacccgaca agtcactctg
    15841 atcttgctga aaatcatcta gatttctgag ttctacactc tcgacaccta attgatctag
    15901 cacttttgca tgttctgcaa aggccccttg caaggccaat attccgattt tcatctattt
    15961 tcctcgttca gccatgagaa tttggatttc attttcatta ataccaacca tggcttctcc
    16021 taaatcttca gagatttgag ctaggatttg aggattacgg aagttagtca cagccttaac
    16081 aatggcactc gctcgtttaa caggatctcc tgacttgaaa atacctgaac cgacaaagac
    16141 cccctctgcc cctaattgca tcattaacgc agcatctgct ggcgttgcaa cacctccagc
    16201 agcgaaattt acaactggca attttccatg ttcatgaaca tattggacca attctacagg
    16261 gacttgcaaa tccttggcag caacataaag ctcgtcctca cgtaagtttt gaatgcggcg
    16321 aatttcctga ttcatcatac gcatatgacg aacagcttgg acgatatccc ctgtccctgg
    16381 ttctcctttg gtacgaatca tggaagcacc ttcagcgata cgacgcaagg cttcacccaa
    16441 atccttagca ccacagacaa aaggaacttg gaattctttc ttgtccacat ggaaacggtc
    16501 atcagctgga gatagaactt cactctcgtc gatataatca atttcaatag cctctaaaat
    16561 ctgagcttca acaaaatgcc cgattctgac cttagccatt actggaatac taaccgcttc
    16621 ttggatttcc ttaatcatct ttgggtcgct catgcgggaa actcctccag ctgcacgaat
    16681 atcagccgga attcgttcca aggccatcac agctgccgca ccagcagctt ctgcgatacg
    16741 agcctgttca ggattctgca catccataat aacaccaccc ttgagcatct gtgccaagtt
    16801 tttatttagt tcataacgat tttcagtcat ttgttttatc ctcatcattt gtattggtag
    16861 ctaccttttc attttaagtc agaacagaat gaagcacaag ataaaaaaac tataattgta
    16921 tggggacaaa ttagctaaaa actatctgac cttaacttaa ggccagatag ctcattcatt
    16981 tttatttttc agccgtaagg gcagccattg tgatgtagtt gtatggtttg ttgaagtgtg
    17041 gcaagaagaa gaggtctgtc aatgccaatt tatcaattgt cacatgctct tggatagcaa
    17101 gtgagaacat gtggattccc atgctaattg caatatcatg tgaaaccatt tgggcaccaa
    17161 gaatttcacg gctatctttg tcaaagacaa tcttaattgc tacttcatgg ttgtcatgtt
    17221 tcatgaattc tggtttttga agatcgttaa agcctgtttc agttgcgttg taaccagcag
    17281 ctttcgcttt ttcaagagtc aaaccagttg aaaccatgtg aagaccgtag attgagatac
    17341 catttgaacc ttgaacaccg attccttcca attcatgtcc acaagcattg taggcaccaa
    17401 cgataccagt gcgaacagca tttgaagcaa gagcgatata gcttgtatct ttacgagcat
    17461 tgtcataaac agtcgcacag tcaccaacag cgtaaacgtc tgggatagat gtttcttgtt
    17521 tcttgtctac aaggaaggca ccgttgcgga agagttcgat cttaccacct gcaagggctg
    17581 tgtttggacg gaaaccaact gcaaggataa ccatatccac gtcaaagctt tctttgtcag
    17641 taatcaagcg ttcaacttta ccgtcacctt cgattgcttt aacagtttga cctagagcca
    17701 agcggatgtt gtgatcttcc aagttcttcg ccatcatttg tgtgaagtct ttgtcatagt
    17761 aaccgttcaa gacagtatca acgatatcaa caaggacaac ttcttttcca agacgttcaa
    17821 aggcttcagc aagttcaaca ccgatgtaac caccaccaac aacggcgata cggtcgaggt
    17881 gttggctctt gtcagaaagt ttattgataa cttcttcagc attttggtac aatttcacga
    17941 attgtacgtt ttcaagagtt gctttaaatt cgcggtttcc tttaacaatt tcaacacctt
    18001 cgattggtgg caagattggt gtagagcctg tagcgaaaat caatttttcg tatgattctt
    18061 tgtgctcttt tccttcaact tccgctgtaa ctactttgtt atcatagtcg attgaaagaa
    18121 caggtgagtt catgtaaact ttagcacctt tagcttccaa tttttcttta tcagaataga
    18181 acaagccttc agcaccgtca atttgttcac caatccaaag agccattcca catcctagga
    18241 aagagatgtt agagttttgg tcaaatacaa caatttcgtt ctcatttcca aaattatcca
    18301 acatggtatt gatacatgct gtaccagcgt ggttagcacc gactacaacg attttactca
    18361 taaaaatttc ctgccttcat tctaaattta ccttactagt ataccattta ccgttagcgt
    18421 ttacaagggg ggtactatta tttcagcatt ttttatcaaa aataattccc catttatcct
    18481 gttttcaagc ctgatgaatt ttatgagcat atttcttgac tttttccaaa ttttgtttgt
    18541 gaaaattcta actttatggt attataataa catgaaaatg aaatgtaagg gcttcaattt
    18601 agatgattat ttgatattac aacttatttt ctataagaga aaggagttgg tagcttgact
    18661 cttcgttcac attctgaacg gctaatgggg actactatca ctatttcatt agtggatgag
    18721 caagccgata tctttctcca aaaatccttt gacttgctca aagaacttga ataccgcttc
    18781 aacgccaata gtcaagaatc tgagttgatg gaaatcaatt atcaagcagg aatagccccc
    18841 gtcacggttc atccagacct gtttgagctg atttcacttg ggttagagca tagcctagcg
    18901 ctctctagcc acctcaatat cagcattggt cccttaattc aaacctggcg tatcggtttt
    18961 tcagatgcca aggtcgccca gcctcaagaa attgaatcgg tgctgcctct aatcaatcct
    19021 catggtatcg agttagattc ttctacttct actgtgtttt taaaacagaa aggaatgaag
    19081 atcgatctag gttgtttagc caaaggatac agtgcggata aggttgccca atttcttagg
    19141 aaagaggggg tgacttctgc cttgatcaat ctgggaggga atatcctgac cattggaaaa
    19201 aatcaggcaa gaggggataa cccatggcaa atcgggattc aagacccagc caatcctagg
    19261 ggaaatcatt taatgaccat ccctgttgtc aataaatctg tcgtgacttc aggcatttat
    19321 gaacgtcacc tgaccgtcga tggacaagat taccatcaca tttttgacag tcaaacagga
    19381 tatcctgttg aaacggaact agcgagtcta acaatcatct ctgataaatc agtcgatggc
    19441 gaaatctgga cgactcgact ctttggagaa agacctgctt ctatcctctg gcaagtcgaa
    19501 agtttggagg gcatcgaagt catcctcatc gataaagaag gccacctaag ctgttcttca
    19561 ggaattccta ctctatagag acaactgttt caatggaatt ttaaaattgt attatgtaaa
    19621 aagagaaagg aaatctcatg ttaaaactta ttgctattgt tggaacaaat tcaaaacgtt
    19681 ctacaaaccg tcaattgctt caatacatgc aaaaacactt tactgacaaa gctgaaattg
    19741 aacttgttga aatcaaggcc attcctgtct tcaacaaacc agctgacaag caagtacctg
    19801 ctgaaatatt ggaaattgct gctaaaatcg aagaggcaga tggcgttatt atcggtactc
    19861 ctgagtatga tcactctatt ccagctgttt tgatgagcgc tcttgcttgg ttgtcttatg
    19921 gtatttaccc acttttgaac aaaccaatca tgattacagg tgcttcttac ggtacgcttg
    19981 gttcatctcg tgcccaattg caacttcgtc aaatcttgaa tgctcctgaa atcaaggcaa
    20041 atgttcttcc agatgaattc ttgctctcac actctcttca agcatttaac ccaagtggcg
    20101 acttggttga ccttgatgtt atcaagaaat tggatgccat ctttgatgac ttccgtatct
    20161 ttgtaaaaat cacagaaaaa ttacgtaatg cacaagaatt acttcgcaaa gatgctgaag
    20221 actttgactg ggaaaatttg taagatagga gaccaaaaag aatgaaattt gttggacttg
    20281 ttggatcaaa ctacgatcaa tcatataacc gtaagctctt ggaatttatc cgtcgctact
    20341 tcaaactcaa atttgaatta gaagttcttg aaattgacga agttccaatg tttaaccaag
    20401 atgaaaaatg ggatgaaagt ttccaattac gttatttata taacaaaatt actcgtgcag
    20461 atggtgttat cattgctact cctgagcata accacactat ctcagcttca ctcaaatctg
    20521 tacttgaatg gctttcatac gaagttcatc catttgaaaa caagcctgtt atgattgtgg
    20581 gtgcatcata ctatgaccaa ggaacatcac gtgctcaagt tcaccttcgt aaaatccttg
    20641 atgctccagg tgttaatgcc tacacgcttc caggtaacga attccttctt ggtaaagcta
    20701 aggaagcttt tgataacaac ggaaatatca ccaacgaagg cactgttaaa ttccttgaaa
    20761 cttgcttaga taactttgtt aaatacgtag gagtcgtttc gaaattgaaa aaaccaaaac
    20821 caatcgaatc agaagacttg gattgtggaa aaccaatcgc tacaaccatc acagaagttg
    20881 accctgacga tccagaatgg gtagaaaaag ttgcagcatt cactggtgct gtttctggtg
    20941 atacctatgt caaattggac cacggtatcc taacagttaa ccaaattgat atgttcttga
    21001 aagctatgcc atttgaattg acatacgctg acgacaacaa ccaattcctc tactacaaca
    21061 acgctcacca agatccagac actatgttcg ctaaacgtgt accacctcaa tcaggtagcc
    21121 gtatgtcgac tgttcatggt tctcttccac cagcacgtat gaagaacgta gagtgggtta
    21181 tcggaacact tcgcaacgga aaccaagaat acgtccgtac gatcgttcca ggttctcctg
    21241 caggtgttat caacactcac aactaccaag caatgtacta tcctgatgga tcatacgctg
    21301 gtatcaatga aatcgtcttt aacttccaac catggcttga ctggtaccta aaagagactg
    21361 gtcaacgttt ggtgggtggt agcggaccat ttgctcctgc tgccggaggt catggagacg
    21421 ccgatgctac ttctggcgct tctgattcag gggatgctgg aggccacggt ggtgacgcag
    21481 acgctacagc cggtgcaagt tactaatgat agtttttagg aaccattatg gttccttttt
    21541 tagcgaaaaa agactagcaa acacaaactg ctagtctctt tgttatcgtt tcatctatct
    21601 ttgttaaaag aaagaataca tatactcaat gaaaatcaaa gagcaaacta ggaagctagc
    21661 cgcaggctgt acttaagtac ggcaaggtga agctgacgtg gtttgaattt gattttcgaa
    21721 gagtattatt atgaatttgg atcatcaaga ctacggccat gtaatccttt ttcacgttgt
    21781 acttggcgta gtttttctgg tgtaacatcg tttccttctt cgtccacaat tttgattcct
    21841 tcaatgtggt gacgaacagc gcggcgataa ccttcgatgt actcctcacg tagtttggct
    21901 tgttccactt tttcttctgg tgttaagcct tctgtttttt tctttttagc aagctcattg
    21961 atacgagcaa tttttttcgg atccatttct tctcctttgt aataagataa agtccgttta
    22021 tcaaatttta tttagtgtta atttggttaa aacaagcaaa cttagctgct ttcttggtta
    22081 gttgtgacaa gattgccaaa cgattttgac ggacagcctg atcttcagcc attaccatag
    22141 tattttcaaa gaaagcatca atgactgggc taagcgcaaa aagttgtttc aattgctgac
    22201 ttgcaggtcc tgataaaatg agtgtttcta ctgcttctgc caaagctttt tcttggtcat
    22261 tctcaaatag tgctgaatca accgtagcaa ccccttctgc cttctcggcc aggttaaagg
    22321 cacgagaaag tgattcaaca gatggtttaa aatcttcttc cttgcttact tctacgagag
    22381 cacttgctgc ttccaacata tctgccacaa caaagtttga acctgcaaga actgcttcct
    22441 tgatatcttt tggagtagag cccatcatct tatcaacacg agccttgata aagtccataa
    22501 cctctgcttt attttcataa gtcaaactgt caaatttcaa tgcataaagg ctatcaatca
    22561 gctcatccat agcaatgtgc caaccaaagg catccaagat acgaaccaca ccttgagttg
    22621 cacgacgaag ggcataaggg tcattagaac ctgatggaat caatcctact gagaagaaac
    22681 tcaaaatcgt atccaatttg tctgcaatgg ctagaactgc gccgaccttg ctctctggaa
    22741 gttctccttc agctgatgta ggcatgtagt gttcacgaat agcagctgcc accgctggag
    22801 tttcaccagc aagaagggtg tatttttcac ccataattcc ttggagttcg tcaaattcac
    22861 caaccatacc tgtcaacaag tcaaacttgt aaatggctgc tgcacgagca aggtcaactg
    22921 tttcatccac tgacaaacta gctttttctg ccaaaagtac agtgatttga cccgtacgaa
    22981 tcatgtgttc acgaagagaa ccaatcttct catggaaggt gacattgttt aatttttcaa
    23041 caagatctga aatcaccaat ttttggtctt cacgccagaa gaattctccg tcttccaagc
    23101 gggctaccaa gactttttca tttcctttga tgacattttt caaacgctct gcgtttccgt
    23161 tacgaacaga aatgaagttt ggcaagagtt ttccatcttg atcacgaaca acaaagtaac
    23221 gctggtgttc cttcatagaa gtcaccaaga cttcttctgg aacttcaagg tatttagcat
    23281 caaaacttcc catgaaggca gttgggtatt caaccaaatt caagacttca ttcagcaaat
    23341 ccgcatcaat ttcgatacgt acaccatgtt ttgcctcaat ttccttgatt tggtcaacaa
    23401 tcatttgctc acgttcacat ggatctgcga ttacaaactg cttacgaagg tcttcttcat
    23461 agctcaatgc tgactgaatc ttggtttctt gtcccaaaaa acgatggcca cgactcacac
    23521 gacttccctt gatatcaagg aaatccaagt caaactcttg ctcatccaag agaacagtta
    23581 aagtgtgaac agggcggatg tattcaaagc tatttcccgc ccagtgcatg ctgacaggga
    23641 aagtcagtga cttcaagaca tccacaatgc ctggaacaat ggcttcaact gcttgaccaa
    23701 tttcttcctt agtgacatag acatattctt cacccttgat ttcacggaat tcgatatctt
    23761 caacagtcaa acctttccca cggacaaatc cttgagctgc tttggtgaag tttccatcac
    23821 tatctaaggc aattttcttt gctggaccct tgaaatcttc tgttaaatca gactgtttgt
    23881 ctgcaagacc agttacacga acagccaaac gacgtggtgt tgagaaagtt tgaatggctt
    23941 caaaagacag gcgttttccc ttgaggaagg ctgccatttt ttcgcctagt tgtttttcac
    24001 ttggcgtaac aacataggct ggtaattctt caagaccgag ttctactaat aagttttttg
    24061 tcatgttttt tctccgaaaa gtatcttttt attttccagt ttgccttatg tgattggcac
    24121 gcaagcaacc aacttcgttg tttcattgct aaaattggag ccaaaagtct ccaattttgc
    24181 ctagtatcct tagtttcact aaggatacct tcatcactgc ggcaactggc tcagggctca
    24241 ctctgttcgc ccattttcgt aatttgtcac tctctttatt ctgcgtcttc tgctaggagt
    24301 ttagctcttg tttcttcatc caaaagtggg tagcctaggc gtttgcgttc tgcgacaaag
    24361 gttttggcta cgacacgggc caagttacgg atacgagcga tatagcctgc acgctctgtt
    24421 acagatacgg caccacgcgc gtcaagcaga ttaaaggtat gtgaacattt gagaacatag
    24481 tcataggcag ggtgtaccaa gccttcttct aatgcacgac cagcttcttt ttcaaactta
    24541 tcaaagtttt caagcaacat ttcttggtcc gaaatttcaa atgaatattt tgagtgctca
    24601 tactcaggct ggataaagat ttctccgtat tttacaccat cagcccactc gatatcatag
    24661 acagagtcta cttcttgaat gtaagaagcc aagcgctcca aaccataggt aacttccgca
    24721 gtcacagggc cagttgccaa tccaccgact tgttggaaat aagtgaactg agtgatttcc
    24781 attccgtcaa gccaaacttc ccaaccaaga ccagctgaac cagttgatgg gttttcccag
    24841 ttgtcctcaa caaaacgaat atcgtgctcc aaaggattga ttcccaattt ttccaaagac
    24901 tcaaggtaaa gttcttggat atttgatgga gaaggcttca tgaccacctg gaattggtgg
    24961 tgttggtaga gacggttagg gttttcccca taacgaccgt cagcaggacg acgtgatggc
    25021 tctacataag ctgcattcca tggctcaggt ccgatagcac gaaggaaagt gtaaggactc
    25081 attgtccccg cacctttttc attatcataa gcctgcataa gcatacaatc ttggtcattc
    25141 caaaattgtt gcaaagtcaa aataatttct tgaaatgtta atttcttaga cattgaatac
    25201 tccttaataa aaaataattt cttgcgacac gagtctgcct cgtcgattat acgaggcaag
    25261 acgagttagt actgcgtcta gctcaggcac tctagtgctg agcgtagggc agtccgactg
    25321 taaggaggtt tctgccactg acgcagtgaa atgttaattt cttagacatt gaatactcct
    25381 taataaaaaa taattacttg cgacacgagt ctgcctcgtc gatcatacga ggcaagacga
    25441 gttagtactg cgtctagctc aggcactcta gtgctgagcg tggagcagcc cgactgtaag
    25501 gaggtctctg ccactgatgc agtgaaatgt taatttctta gacattgttt actcctttag
    25561 tttttatatt tctttttcaa ttaatttctg ctgaacaaac caatctaaca agggaaaagt
    25621 tttttctgta tttgcccata aatgataaga atctaagacc gcttgtacac tacctaaatt
    25681 ttctaccaac ttatctactg tcaaaaagct gcgccagtat tcataaaaaa tacttgcata
    25741 gttcttatca taagtcgaag aaccaaaatc attcaatgaa tgccaactat acttcttctg
    25801 aaaaagttct acgagagatt gattacaaat tttttccgct tgaaattcct cttctgtcaa
    25861 gaaatacttg cgactaatat attcaaccat cccctcttcg aaccagatat aagagtcata
    25921 accatcaaaa tcatctaaaa aatgtccgac caatgagcta actcatgtcc tacaatctgt
    25981 aagaggaaat tttcagataa agaatgatag tgactttcta ttgcttgagt ttgctcagaa
    26041 cactcgtagt ccatcaactg atacaaatac aattctttcc aaacagctaa atcaggcgtc
    26101 ataaccactc gtctattatt tgtataggtt ggaacagcac tttccctaat aatctgtgta
    26161 gcagcattaa aatctgacca aataacagct tgcggtaaat cacagactgc aagttcattc
    26221 tttaaaaaag attgataatc tttcaacttt tcagtatttc ttactacaaa atcacgaaat
    26281 gtagctagtt gactatcgtc ttttacaaga taaaggttgt ccacggctct cctttctttc
    26341 acacaataga ctgaataagc aaaagaacca catctgacaa tcctaggttg atttaaccta
    26401 ggggcgtcag aaacgcggtt ccaccctaat ttattacttc attgtttttg aaaatactac
    26461 agaaagcgcc agccctttat tttgccctac ttggctccca ctatcccaag ctcgcttgaa
    26521 gaacgccata agactttctt tcctgctgac tattatatca aaaattagaa taatgtacaa
    26581 ttctttttat gattttctag aaatccacat catcaactcg tggagcaccc gattgaacag
    26641 caatcgtttt taaggtttct ctctcctcat gactcagttc aattccaaag caatcaagat
    26701 tagcttgaat acgagaggtt gtgacagatt ttggaagtgg taaaaatcct tctgccaagc
    26761 tccaggccaa ggctatctga gcaaccgatt ttccgtgatt tgctgctatt tcttggactt
    26821 gcttgctatc aaacagttct ccttgtccaa aaggccccca agcttccaat aaaattccct
    26881 tttcacgaca gtaagctacg acttgatctt gatacacacc tggcgccaag cgaacttgat
    26941 tgaccgcagg aacgatagtt gcagtttcaa gcaaggcatc caaatgatgg ggaagaaaat
    27001 tgctaacgcc gatagcacgg attttccctt cttgatagag gtcttccatc gctctccaaa
    27061 cttccgcatt gcgagttttc catgcgtcat tttctctgag cggttttggg ttcggccaat
    27121 gaatcaaata caaatccaaa taatccaagc ccagtttttc tatagatttt tccaaagctt
    27181 gacgagtttg ctcataggtt tgctgactat tccaaagctt ggtagttacg aacatttctt
    27241 cacgtggaac tccgctatct ttgattgctt gaccaacact ttcttcattc tgataaatcg
    27301 ccgccgtatc aatatgacga taaccagcct tcaaggcttc taacactgca cgataggctt
    27361 cttctccatc cttagcctta aaagttccaa atcccaatac tggaatttct actccattat
    27421 ttaattgata ggtattcata tgcttctcct tctattttat tcatcaatta tagcataaaa
    27481 cattttttaa gtagtactat attctgtaaa aaataagtct gtcaaatatc taacagacct
    27541 attttttctt gcttattcat cacgactata gtacagctca tgagctttta ggcgagtatt
    27601 gagcatctct ggtatggtca caaaggtata accttgattt ttcaaatact caatgaccct
    27661 tggcaaggca ttgactgtcg gactgtgaat atcatgcatc aaaacgatag agccattagc
    27721 tacttgatac tgaatttctg tcaaaataga tgcttcattt ttactcttcc agtccagact
    27781 atccacatcc cacatgataa agctcaaatc caagctattg cgaatatcat ctgtaatagc
    27841 accataaggt ggacgcatga gtttagaact agaacccagc actttagtta gcacatcctc
    27901 agtatcagta atctgctttt tagcttcatc aagagagagt tgcgagagaa tcggatggct
    27961 ccagctatgg tttccaacaa catgaccttc agattttatc ctcttcacca agtcctcatt
    28021 cccagaaaca tttttcccaa gcacaaagaa agtcgcttta atatcatatt tagctagggt
    28081 ctctaatacc tgcggggtcg ttgctggatt tggaccatca tcaaaggtta gagcgacaac
    28141 tttttgatgt ttcttatcaa agtaagattg gtacaaggcc gcatcttttt cgagtaagta
    28201 cgaagattgg ataacatcaa agaaagcaga tactggcaag gctatctctt ctaaattttc
    28261 aaccacagga cttggataaa ggataatctg actatccttg taatcaaaat tccatgcaga
    28321 caagtcttgg tcagagaagt tttttacaat ctgctcactc tggtcttgct ctattttttt
    28381 atcctctatg aaggaggtca actcttttat cagctgttcc ttagccttac tagcatctga
    28441 aaacagttgg tcaagtgtaa aaggttgccc atcttctgtt aagtgaacct ttcctagact
    28501 ggtcttttca gtctcttcaa tcttaaaagc aactaaatca tagatttgtt tggtcacatt
    28561 acgattaacg actcccttta aacctgactc ttcttgctct gtatagtaga aaacaagatt
    28621 gtccttactt tctagcttgt ccttaacatc ttgatttatc agctccctaa cagaggaaat
    28681 cactttctcc ccttggagag gataataggc aatcacttcg gcttgcccct gacgaaaatg
    28741 ctccttctga tttccctcac tcaattgatc atctttctct tttttgagcg attcaatctt
    28801 ttgctcaaaa cttttttgct ggtagatctt aaaagcaata aatccgccta ataggcaaat
    28861 agaaattgct aaaataccaa tcaaagccaa taatacatgt ctcgttttcc cgtgtctgcc
    28921 acgtcctagt ctacttttat tcatatttat atcataacaa atttgagcaa taatttcaat
    28981 gatacaattt ggcaatccac aattcctcta gcaagccaaa gcatcttaca tcaactccaa
    29041 catccgtatc tgtagagatt atgtagtttt ctatctatgg aatttctagt agattgaagc
    29101 tagaatagta gacctatact tctaaaacat tgttagaaat cgatttgact gtcctgatcg
    29161 atttgtcctg ttctcatttc attttactat aaaactatga aaagctaggt catctgagaa
    29221 gtaattacta tcgacaattg cgatagcgta tactagtgcg atttgtttgt agctctggtg
    29281 ggtatcacct tcacgttggc gaattttttc taggttttga ttaacctgac tgcacagata
    29341 aggccatagg cgattgatga aaaaattctg atgatgaacc tgaatttcga tgataacttg
    29401 gattccatta tctaactccg ccaagacgtc tatactagta tagaaatctt acgctgagta
    29461 gggcatggaa ggcaagacgt gaatgttact tccctccaaa atcgtcacat tttttgctgg
    29521 caagtccagc atatcgcgga taaattgaca aatcattagt tgggctgata cctagatgtc
    29581 tgagaatcct ccttttcctc cttctattat agcacatttt taaatctagg tttgctagat
    29641 tctatgcttc tatctattta ttcggaaaag aaagctaata gcttatttga aattttctcc
    29701 tctctgttgg atagtcccgt ctatctatgt tataatgaaa gaaggattta aaatcggaaa
    29761 aggagtattt atgctaaaat taggtgtcat cggaactggc gctatcagcc atcatttcat
    29821 agaagcagcc cataccagtg gagaatacca actggtcgca atctattcta gaaaactaga
    29881 aactgcagca acctttgctt ctcgctatca gaatatccaa ctattcgatc aattagaagt
    29941 cttcttcaaa tcttcttttg atttagtcta tatcgccagt ccaaactcct tacattttgc
    30001 tcaggcaaaa gctgccttgt ctgctggtaa acatgttatt ctcgaaaagc cagctgtctc
    30061 tcaaccacaa gaatggtttg atttgattca aacagctgaa aaaaataact gttttatctt
    30121 tgaagcagct cgtaattacc acgagaaagc ctttactact atcaaaaact ttttagcaga
    30181 taagcaagtg ttaggagcag atttcaacta tgccaaatac tcttccaaga tgcctgactt
    30241 gttggctgga caaacaccaa atgtcttttc agaccgtttt gctggtggag ccctcatgga
    30301 tttggggatt tatcccctct atgctgctgt tcgtcttttt ggaaaagcca atgacgcgac
    30361 ttaccatgct caacagcttg acaatagcat tgacctaaat ggagatggta tcctcttcta
    30421 cccagactat caagttcaca tcaaggctgg aaaaaacatc acttccaatc ttccttgcga
    30481 gatttataca acagatggaa ccttgactct caacacgatt gagcatattc gctcagctat
    30541 ttttaccgac caccaaggaa atcaagtcca gcttcctatc caacaggctc ctcatacgat
    30601 gactgaggaa gtcgctgcat ttgcacacat gatccagcaa ccagatctga atctctacca
    30661 aacttggctg tatgatgcag gctctgttca tgagctacta tataccatgc gccagactgc
    30721 tggcattaga tttgaggcag aaaaatgaaa accaaactac cgactgaatg gcaagaactg
    30781 agtgaccaac tcggtttcca agaattcacc cccattcaaa ctcaactatt tgagcccctt
    30841 cttgctggag aaaacctcct aggagtgagc caaaccggaa ctggtaagac cctagcgtat
    30901 ctcctaccaa gtcttctcag actacaaaag aaaaaagccc aacaactctt gattctagca
    30961 ccaaatacgg aacttgctgg acagattttt gatgtatgta aaacgtgggc agaagctatc
    31021 ggattgactg ctcaactctt cctttcaggt tcaagtcaga aacgacaaat tgaacgtctt
    31081 aaaaaggggc cagaaattct gattggaact cctggccgta tctttgaact cattaaactt
    31141 aaaaaaatca agatgatgaa tgtggaaacc atcatcctgg atgaatttga ccaattacta
    31201 gatgattctc agattcactt tgtagaaaaa atcactcact acgcacctcg tgaccaccaa
    31261 ctggtctaca tgagtgccac gaccaagttt gaccaagaaa agattgtgcc gaatacacgc
    31321 actattgatc tctctgatca aaaattggac aatatccagc atttctacat gcaggtagac
    31381 caacgtcatc gagtggatat gctacgaaaa ctggctcatg tagaggattt ccgtggtcta
    31441 gtcttcttca atagcctgtc agaccttggg aatgccgagg aaaaactaca gtatcgtgat
    31501 atattggctg tttccctcgc tagtgatgtt aatgttaaat ttagaaaaat catcttagaa
    31561 aagtttaaag ataaccaact aaccctgctt cttgcaactg accttttggc tcgtggaatt
    31621 gatatcgata gcctagaatg tgtcgtaaac tttgatattc ctagagatag cgaaacctac
    31681 acacaccgtg ctggccgtac tggtcgtatg ggcaaggaag gttatgttat cactctcgtc
    31741 actcatccag aagaaatcaa aaaacttaaa aagtttgcaa gtatccgaga aattgtccta
    31801 aaaaaccaag aactctatat caaataaagc cccattgctc tatacacatc cagtgtatgg
    31861 agctttttta taagcaattc aattttatac tcatctgttt tcaaaaagca ttctagtcca
    31921 tctccgatta acgatggact ttatcacctc cttctccaat ccttgtatga catcttggag
    31981 ttgattcatg acatcttcca aagttcgaaa ggctttattc ttaaatccac gtttacgaat
    32041 ctctttccac acttgttcaa tgggttcatc tctggtgtgt atggaggaat aaaggtaaaa
    32101 ccaatattag tcggaatctt taaggtactt gatttatgcc atatagcatt gtccataacg
    32161 agtaaaagat aatcatctgg ataagcttgt gaaagctctt ctaaaaaggc gttcatccac
    32221 tcagtattac atccaccagc tattaagaaa aatgattcgc ctgtatgggc atcaacagct
    32281 ccataacaat agcgaaattc tcgtatatag tgactatgga catgtggacc tactcctatt
    32341 ggagaccaac aagatcccag tttactgatt ctaccgaaac cagcctcatc ttggtacatc
    32401 aagcgaacct tatgaaaagg tctactggtt ttaaagcgct ttcttgtgta ctgcccccaa
    32461 acagttagac aaataattta tccaaaggat ttagttctgt actgcacagg actaagtcct
    32521 tttagtttta tcttaatttt cttattgttg ta
//
LOCUS       DAVKNL010000022        31160 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVKNL010000022 DAVKNL010000000
VERSION     DAVKNL010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31160
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.022"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            108..1619
                     /locus_tag="VW411_000991"
     CDS             108..1619
                     /locus_tag="VW411_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEU9793742.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNTWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            1641..2972
                     /locus_tag="VW411_000992"
     CDS             1641..2972
                     /locus_tag="VW411_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU9793743.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     LLYEQVCNGLKEAGYDYQTTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAEDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            complement(3378..3668)
                     /locus_tag="VW411_000993"
                     /pseudo
     CDS             complement(3378..3668)
                     /locus_tag="VW411_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3656..3907)
                     /locus_tag="VW411_000994"
     CDS             complement(3656..3907)
                     /locus_tag="VW411_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU9793744.1"
                     /translation="MKEFQFERKQRFSLRPYAIGACSVLLGTSLFFAGMGAQPVQDTE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            4111..4188
                     /locus_tag="VW411_000995"
                     /pseudo
     CDS             4111..4188
                     /locus_tag="VW411_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(4306..5190)
                     /locus_tag="VW411_000996"
     CDS             complement(4306..5190)
                     /locus_tag="VW411_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU9793745.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(5335..6486)
                     /locus_tag="VW411_000997"
     CDS             complement(5335..6486)
                     /locus_tag="VW411_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEU9793746.1"
                     /translation="MAIFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKTLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            complement(6632..8398)
                     /locus_tag="VW411_000998"
     CDS             complement(6632..8398)
                     /locus_tag="VW411_000998"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEU9793747.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEASDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERALKWVKENVKEGFDHNREDLVLSREEKDRQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPIMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            complement(8481..11597)
                     /locus_tag="VW411_000999"
     CDS             complement(8481..11597)
                     /locus_tag="VW411_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEU9793748.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVSFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDPNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKEDSPSWWEIDLAQTEELEKLIIYNRTDAE
                     IQRLSNFDIIIYDSNDYEVFTQHIDSLESNNLSIDLKGLKGKKVRISLRSAGIPLSLA
                     EVEVYTYK"
     gene            complement(11607..13901)
                     /locus_tag="VW411_001000"
     CDS             complement(11607..13901)
                     /locus_tag="VW411_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEU9793749.1"
                     /translation="MKLWYKKAAANWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTVFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKGCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPKTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQEREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            complement(13960..14760)
                     /locus_tag="VW411_001001"
     CDS             complement(13960..14760)
                     /locus_tag="VW411_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9793750.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            complement(14757..15530)
                     /locus_tag="VW411_001002"
     CDS             complement(14757..15530)
                     /locus_tag="VW411_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9793751.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            complement(15555..16025)
                     /locus_tag="VW411_001003"
     CDS             complement(15555..16025)
                     /locus_tag="VW411_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9793752.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            complement(16016..16447)
                     /locus_tag="VW411_001004"
     CDS             complement(16016..16447)
                     /locus_tag="VW411_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9793753.1"
                     /translation="MFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVDELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISSYDSKINLEEIVHNAQNSLFNVKQQLNVEEEEDLCL"
     gene            complement(16437..16880)
                     /locus_tag="VW411_001005"
     CDS             complement(16437..16880)
                     /locus_tag="VW411_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HEU9793754.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENVQ"
     gene            complement(16892..17530)
                     /locus_tag="VW411_001006"
     CDS             complement(16892..17530)
                     /locus_tag="VW411_001006"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HEU9793755.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMELMAEKFKTYGQRK"
     gene            complement(17645..19048)
                     /locus_tag="VW411_001007"
     CDS             complement(17645..19048)
                     /locus_tag="VW411_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEU9793756.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLLQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKEE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGLTEATAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            19224..19997
                     /locus_tag="VW411_001008"
     CDS             19224..19997
                     /locus_tag="VW411_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9793757.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTVPDQKLLDAVELFDGEWVSDIQ"
     gene            complement(21157..22662)
                     /gene="adcA"
                     /locus_tag="VW411_001009"
     CDS             complement(21157..22662)
                     /gene="adcA"
                     /locus_tag="VW411_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEU9793758.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYHTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNVAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(22672..23478)
                     /locus_tag="VW411_001010"
     CDS             complement(22672..23478)
                     /locus_tag="VW411_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU9793759.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIVLRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            complement(23471..24175)
                     /locus_tag="VW411_001011"
     CDS             complement(23471..24175)
                     /locus_tag="VW411_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU9793760.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYCVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            complement(24175..24615)
                     /gene="adcR"
                     /locus_tag="VW411_001012"
     CDS             complement(24175..24615)
                     /gene="adcR"
                     /locus_tag="VW411_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEU9793761.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            complement(24770..26038)
                     /gene="dltD"
                     /locus_tag="VW411_001013"
     CDS             complement(24770..26038)
                     /gene="dltD"
                     /locus_tag="VW411_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEU9793762.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMNYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            complement(26031..26270)
                     /gene="dltC"
                     /locus_tag="VW411_001014"
     CDS             complement(26031..26270)
                     /gene="dltC"
                     /locus_tag="VW411_001014"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEU9793763.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            complement(26284..27528)
                     /gene="dltB"
                     /locus_tag="VW411_001015"
     CDS             complement(26284..27528)
                     /gene="dltB"
                     /locus_tag="VW411_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEU9793764.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIVIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMWGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            complement(27525..29075)
                     /gene="dltA"
                     /locus_tag="VW411_001016"
     CDS             complement(27525..29075)
                     /gene="dltA"
                     /locus_tag="VW411_001016"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEU9793765.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLASFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEDLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            complement(29096..29227)
                     /locus_tag="VW411_001017"
     CDS             complement(29096..29227)
                     /locus_tag="VW411_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEU9793766.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            complement(29541..30719)
                     /locus_tag="VW411_001018"
     CDS             complement(29541..30719)
                     /locus_tag="VW411_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9793767.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTCINLVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMEIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
BASE COUNT         9527 a         6598 c         5265 g         9770 t
ORIGIN      
        1 gactttaata tggtaaaaca aatataaata gaaagcgttt tcttgaatgt ttatttaaga
       61 aagtagttgg ttttttacac tttgttagac atcaggagga aaaacaaatg agtgaaaaag
      121 ctaaaaaagg gtttaagatg ccttcatctt acaccgtatt attgataatc attgctatta
      181 tggcagtgct aacttggttt atccctgcgg gggcctttat agaaggtatt tacgagactc
      241 agcctcaaaa tccacaaggg atttgggatg tcctcatggc accgattcgg gctatgctag
      301 gtactcatcc agaggaaggt tcgctcatta aagaaacgag cgcagcgatt gatgtagcct
      361 tcttcatcct tatggttggt ggtttccttg gcattgtcaa caaaactggt gctcttgacg
      421 tagggattgc ctctatcgtg aagaagtata agggccgcga aaaaatgtta attttggtac
      481 tgatgccttt gtttgccctc ggtggtacaa cttatggtat gggtgaagaa acaatggcct
      541 tctatccact ccttgtgcca gttatgatgg ccgttggttt tgatagcctg actggtgttg
      601 caattatttt gctcggttct caaatcggct gtttggcatc tactctgaat ccatttgcga
      661 caggtattgc ttcagcgact gcgggagttg gtacagggga cggtatcgta cttcgtctga
      721 tcttctgggt taccttgact gctcttagta cttggtttgt ttaccgttat gcggataaga
      781 ttcaaaaaga tccgactaag tcactggttt atagtactcg caaagaagat ttgaaacact
      841 ttaacgtaga agaatcttca tctgtagaat ctacacttag cagcaaacaa aaatcagttc
      901 tcttcttatt tgtgttgaca ttcatcttga tggtattgag cttcattcca tggacagacc
      961 ttggcgttac catttttgat gactttaata cttggttgac tggtcttcca gttattggta
     1021 atattgtcgg ttcatctact tctgcactag gtacttggta cttcccagaa ggcgcaatgc
     1081 tctttgcctt tatgggtatc ctgattggtg ttatttatgg tcttaaagaa gataagatta
     1141 tctcttcctt catgaatggt gctgctgact tgctcagtgt tgccttgatc gtagcgattg
     1201 ctcgtggtat tcaagttatc atgaacgacg gtatgattac cgatacaatc ctcaactggg
     1261 gtaaagaagg cttgagcggt ctatcttcac aagtctttat cgttgtaact tatatcttct
     1321 atctacctat gtcattcttg atcccatctt catctggtct tgccagcgca actatgggta
     1381 tcatggctcc acttggagaa tttgtaaatg tccgtcctag cttgattatc actgcttacc
     1441 aatctgcttc aggtgtcttg aacttgattg caccaacatc tggtattgtg atgggagctc
     1501 ttgcacttgg acgtatcaac attggtactt ggtggaaatt catgggcaaa ctcgtagtcg
     1561 ctattattgt agtgaccatc gcccttcttc tccttggaac cttccttcca ttcctataaa
     1621 atagtgagtg aggtgattcc atgaaaatag atataacaaa tcaagttaaa gatgaatttc
     1681 ttatatcatt aaaaaccttg atttcctatc cttcagtact caatgaagga gaaaatggaa
     1741 caccttttgg acaagcaatc caagatgtcc tagaaaaaac tttagagatt tgtcgagaca
     1801 taggtttcac tacctatctt gaccctaaag gttattacgg atatgcagaa atcggtcagg
     1861 gagcagagct tctggccatt ctctgtcatt tggatgttgt tccatcaggt gatgaagcag
     1921 attggcagac accgccattt gaagcaacta tcaaagacgg ctgggtattc ggacgtggtg
     1981 tccaagatga taaaggccct tcgctcgcag ctctctatgc agtaaaaagc ttgctggacc
     2041 aaggtattca gttcaaaaag cgcgtacgct ttatctttgg taccgatgag gaaaccctct
     2101 ggcgctgcat ggcacgctac aataccatcg aagaacaggc cagtatgggc tttgcacctg
     2161 actcatcttt tcctctgacc tatgctgaaa aagggcttct acaggtcaaa cttcatggcc
     2221 ctggatcgga tcaactagag cttgaagtag gaggcgcctt taacgttgta ccagacaagg
     2281 ccaactacca aggtctcctc tatgaacagg tttgtaacgg tctcaaagaa gctggttatg
     2341 attaccaaac cactgaacaa accgtaacgg ttctcggagt gccaaagcat gctaaggatg
     2401 ctagtcaagg tatcaatgct gtcatccgac tagctaccat tcttgctcct ctccaagaac
     2461 accctgctct cagttttctt gcaacacaag caggtcaaga cggcacagga agacaaatct
     2521 ttggtgatat agcagatgaa ccttctggtc acctatcctt taatgtcgca ggtctcatga
     2581 tcaatcatga acgttctgaa atccgtattg acattcggac tcctgtctta gctgacaagg
     2641 aagaactagt agagttgctt acaagatgtg cacaaaacta ccaactccgc tacgaagagt
     2701 ttgactatct agcgcctcta tacgtcgcag aagacagtaa actcgttagc acactgatgc
     2761 aaatctacca agaaaagact ggcgataaca gtcctgctat ttcatccggt ggtgccactt
     2821 ttgctcgcac catgccaaat tgtgtagcct tcggcgcctt attcccagga gcgaagcaga
     2881 cagaacatca ggcaaatgaa tgtgccgttc tagaagattt gtaccgtgct atggatattt
     2941 atgccgaagc cgtctatcga cttgcaactt aatcaggcaa ctgtttctac caaaaaaaat
     3001 cgaccgatta atgaactgca ccccaaaagt tagacagaat aaatctaact tttggggtgt
     3061 tttattatga aattgagtta tgaagataaa gttcagatct atgaactaag aaagcaagga
     3121 caaagcttca aacagctttc aaaaagattt ggtgtggatg tttctggtct aaagtcatct
     3181 gaatctttga gatgagcttt ataaatcgct tttttcagtt tttgcactgg tgtttcgata
     3241 aactcaaact ttttagccgt ggtattgcct gattttatag tatattgaaa ctagaatagt
     3301 acacctctcc ttctaaaaca tttttagaaa tcgatttgac tgtcctgatc gatttgtcct
     3361 gttcttattt cattttacta tatttgagcc acttcgtctt taacggcttt attcataagc
     3421 tcttgtaatt tttctttact atcaattact tctgattttc cgttgtaatt tattgtaata
     3481 ggttttaact tacctaattt ctcgacacgc tcattaattt gatctttttt gaaggctgct
     3541 tatgtttttc ctaagatttt ttcaaaaata tatttatcag atagcggttt gtcttcttct
     3601 tcagcttggt ttttgtatta atttgaaaca taaggaacaa atccttcata gtaacctaat
     3661 gctcccataa gttcaaaagc ttgttttcta attcaaacca ttgcaactca gatttcagct
     3721 tttcagataa atcctgctca tccaaataat gacttgaaat tagtgctgaa ctcgtttctg
     3781 tatcctgtac aggctgagca cccataccag caaaaaataa actcgttcct agcaagaccg
     3841 aacaagctcc tattgcatat ggcctcaaag aaaaacgctg ctttctttca aattgaaatt
     3901 ctttcatccc atctcccatc attcattatt actgtatatt ttgtatatca gaaatagttt
     3961 gtattcacaa atctttctag ttattccctt atcattccta attaagggag ataacataca
     4021 ataattttta gttaaatgta tatcgatgtt ttttgttttt cttaataaac gcaatacaaa
     4081 aagagcctgt taccaagctc tttgtactca atgaaaatca aagagcaaat taggaaacta
     4141 gccacaggtt gctcaaaaca ccgttttgag gttgcagata gaactgacga agtcagctca
     4201 aaacactgtt ttgaggttgc agatagaact gacgaagtca gtaacatcta tacggcaagg
     4261 cgacgctgac gtggtttgaa gagattttcg aagagtatta gtctattatt tcttctcagc
     4321 gcgaagggct gacaagattt gtgttcggat atcatccaca ccatttggag tatttggtaa
     4381 aaagatagtt tgatttcctt tagaggcaaa ggtattcaag gtatccaaat actggttggt
     4441 caagaggata gacatgattt gttcttctgt catgccaaca ttggcttcct tgagttcggt
     4501 gatagactct gccaatccat ccacaatcgc cttacgttgt tgggcaatcc ccacaccatg
     4561 aaggcggtct ttttctgctt ctgcttcagc tgcagtgaca attttaatct tgtcagcttc
     4621 cgccaattct tgtgctgcga cccgcttacg ttgcgccgca ttgatttcat tcatggattg
     4681 cttaacttct gcatctggtt cgaccttggt aatcaaggtt ttcacgataa tgtagccgta
     4741 agtggtcatt tcttctgcta cttggtgttg aacttcaagg gcaatctcat cttttttctc
     4801 aaacaattca tccaaggtta attttggaac agaagagcga agagcatctt cgatataaga
     4861 tttaatctga gattctggac gtatgagttt atagtaagca tctgtcacgc tctgctcgtt
     4921 gacacggtac tgagtcgcta cattcatcat aacgaacaca ttgtccttgg tcttagtctc
     4981 aaccacaata tcactttgca acaagcgcaa ctgaatccgt gctgcaatcg agtcaatccc
     5041 aaaaggcaag cgaatatgaa taccgctatt agcaaccttt tggtatttcc caaagcgttc
     5101 aataatcgcc accgactgct gacgaaccac ataaactgta ctcagtgtga ctatcaccaa
     5161 taggagcaca caaacaatca gaaaaatcat gaaaaatatt gccataatgg aacctccaca
     5221 agtatttttc tagtattata gcacatttaa agaaggctgt gccgttttta ctgcgatttt
     5281 tcctgaaatg tcaataatta gaggtgaatt gtcctattgt cgtccaatct cttgctaaaa
     5341 taactcttta taaaaggcaa tcgtttcttc taaggttggc ataaatggat ttcctggtgc
     5401 gcaggcatca atcaaggcat tcttagaaag gtattcaaag tcgaaatctt tttcttcaat
     5461 accaagttca gtcagtttct taggaatacc tactgtctca gaaagcttct caatctcagc
     5521 aatcgcataa tcggcacatt cttgatctga tttaccttct acatgaagtc ccaaggcttt
     5581 ggcaacattg cggaaagctt ctggtacacg tttagcattt tcacgttcta taactggtag
     5641 caacatggca cagcacacgc catgtggcaa attatatact gcaccgagtt ggtgagccat
     5701 tgagtgaaca tagccaagac cagcattatt aaagctcata ccaccaagga agatggcatt
     5761 gaccatacct tcacgcgctt caatatcatg tccattttct acagcacgag gaagatattc
     5821 cttgataagc tcaatagcac caatagacag tttcttggtt acatcataag caccaggtgt
     5881 taccaaagct tcaacagcat gagtcagagc atccatacca gtagcagcag tcaggccttt
     5941 aggtttggaa agcatgagtt cagggtcatt aacagagata agagcaaggc tattcttgtc
     6001 aaccattacc atcttaacct tgcgttcttc atcagtaatc acatagttaa tggtaatttc
     6061 tgctgatgta cctgccgttg tattgatagc cacaactggc aagccttttt tagcagactt
     6121 atgaagccct tcataatcct gtggttttcc accatttgta gccatgatag aaatacaact
     6181 agctgcatcc tgtggagaac ccccgccaag actgatgata aagtcacagc catgttcttg
     6241 caaagcagct accccatctg tgacattttt acaagtaggg tttggctcta catcgctaaa
     6301 gatgacatat tcgattcctt ctgtatccag tggttttaaa gtcttaggta aaatatcact
     6361 gccttcgatg tacttatctg tcaccaaaag tgccttttta tagccaagtt ccttgatata
     6421 aggacccact tcatttacaa caccttttcc aataaggttg actgccggaa cataaaatat
     6481 agccatataa ttttcctcct gcgcctccgc gctatctatt taacatcatt atattacttt
     6541 ttgtattcgt tttcaattat ttgtgaattg tttaacaaat ctttttaaaa ggataggagg
     6601 gacgtaagtt cacctcctat ataaacaagt tttatttatg tagtggcccc aacaactgac
     6661 atgcacgata gtctgctgat tctagatctt ctgttccaaa taaggaccaa tttttaggtc
     6721 taaagatatc ttcctcaggt acattatgca tattgacagg aattctcaac atagaagcca
     6781 aggtaatcaa gtctgctcca atgtgtccat atgttatggc tccgtgatta gctccccaat
     6841 tattcatgac gtcatagaca gacttgaaag caccttttcc tgtcaaacgt ggagcaaacc
     6901 aagtagttgg ccatcctgga tctgtacgat tatctaaagt atggtgaaca tcttcaggaa
     6961 gttcaagtgt gtaaccttct gcaatttgta gcactggacc aacccctttt agaagattga
     7021 gacgtaccat tgttactggc atatccccct tcgtcaagaa acgagttgag aatcctcctc
     7081 cacggaagta ttcgcggttt gctggtggga agtctgtatt ttcaagcata gcctgcactt
     7141 cactttcttc caactcccag aatggtttca taataggttt gccatctcga gtagcttgac
     7201 ctgtaccatc caatgtacaa gaaccagagt tgattagatg taagaagcca gctgcagcac
     7261 gaccctctaa agtatgtccc gttacacgtt taacagcctc tgggctccaa taagtacgca
     7321 catcagcaaa gatttgtgga gtatttgtta atagataatt aaagagcata gacacaccat
     7381 ttagtgaatc attctctgtc gcaaatacaa atggttttcg aataccattc cagtcaaact
     7441 gagtattgag gaaagtttcc ataaagtccc catttggaaa atggtctgtc cactgacgtt
     7501 gaccttggaa accagctact aaagcatggt gaccaaccgc ttcttcctca aaaccaagtt
     7561 cagcaagtct tgggttacca accattaagt cacgtccaat catgaacatc ttaataacaa
     7621 attcccattg tctatctttt tcttcacggc ttaaaacaag gtcttcacgg ttatggtcga
     7681 atccttcttt tacgttttct ttcacccatt tgagcgcacg ttcgaactct tcagggtcgt
     7741 aaataccacg gtccatacgg cgcgtgaact ccgtcatatc taccgattca tttcgcattc
     7801 ctaagtattc ttggaagaaa tccggattta caatagaacc accaatcccc atcgaaacac
     7861 tacccattga taggtaagca gtgtctctca tcaagccagt tgcaagagct gcacgcgcat
     7921 agcgtaaaag tttttctttg acatcttctg gaatatctgt gtcactagct tcctgaacat
     7981 ctcttccata aatcccaaag gctggaatcc ctttttgagc atgtgaagct agtacagctg
     8041 caagatagac agctcctggg cgttctgtcc cattaaatcc ccaaatagca tgaggaatat
     8101 ctggagacat atccatagtt tcactaccat aacaccagca tggtgtaact gtaattgttg
     8161 cgcaaacatt tgatttttta aacaactcat gggaagctgc agcctctggt acacggccaa
     8221 tagtagatgg agaaatcacg cattccacag gttccccatc tggatatttc aatgtgcttg
     8281 aaatcaaatc tgccacactt ttagccatgt tcattgtttg cacttcaagt gattcgcgta
     8341 caccttgacg acgaccatca atagtcggac gaatcccaat acgtggatgt tgaatcatac
     8401 tattttcctc ttatttttct gtttctttca tactaccatc tccattatat ttacggtagc
     8461 ctgggtgata atttttaaaa ttacttataa gtataaacct ctacctctgc taaacttaaa
     8521 ggaattcctg cgcttctcaa agaaattcta accttttttc ccttcagtcc ttttaagtct
     8581 atggatagat tattgctttc taaactgtca atatgttgtg taaaaacttc ataatcattt
     8641 gaatcatata taataatatc aaaatttgat aatctctgaa tttcagcatc tgttctatta
     8701 taaataatta acttttctaa ttcttcggtt tgtgctaaat ctatctccca ccatgaagga
     8761 gaatcttctt tggtatgagt cactgaatga tgaccataat ctccgttttt atttccatca
     8821 actgctagac gagaaaaacc attgtaatcc gttgaactct gtcgagtttc ttttgttaat
     8881 gcaatattag aaagtttctt cggagttctc tttgaaccat agacttcaac ttccgctaaa
     8941 cttaacggaa tagctgctga ttttaattct attttgacta gcttagcccc tacagaaggt
     9001 acttttaaag ataacaaacc atcaactact ttatcaaaat gtcttctaaa aacttcttct
     9061 cctgatgaag atagaaaaat aacatcaaaa ttagataaac gctgaggttc ggcatctgtt
     9121 ctattataaa tatcaacttt agaaaccgta aactcttctc ccagatcgac ctgccaccaa
     9181 gcgttagaat ctgccttagt atgagtcacc gaatgatgtc cgtaatctcc atctttatta
     9241 ccatcaactg ctaaagcagc tacaccctca taattggttg aactttgagt agctacctta
     9301 tttgtagaga ctactttatc ttctgttatt ttatctatat cctcttcaga ttgcttacca
     9361 tctgattctc taaaaacctc tacttctgct aaactcaaag gcactccact cgttagtaat
     9421 ttaactttaa tataccttgc tcctttttct ttgaaatcta gactaacaga ttcacccgac
     9481 aaattattaa catgtttctt agcaacttcg tttctattat tgtcatatag aatcacatca
     9541 aaattagata gacgttgagt ctcagcatct gtgcgattat aaattttaac aagcccaact
     9601 ttatccattt ttttcaaatc gacttcccac caagagggat tatctgccct agtgtgtgtt
     9661 accgaaccag agttaaaatt accatttctg ttaccatcca cagctctatt agggtctcca
     9721 ttgtaatcta cagaactttg tgttgttggt tttgcatatg ctatattcaa attaccatca
     9781 ttaaatttat ctggtgttgg gaaagagact ggacctgtcc cctcagtatt tatagacatc
     9841 tctaccattc cattatgatt aaccgtaatg gtataaacat gggtattctc atcccaattt
     9901 tctgtataag tataatgatt tttatcgcca cttatattta tctgaggttt atgaccagta
     9961 tgccctttta atgtaagtgt tgttgtccta aagtcattat ctacaggatt gatggaataa
    10021 tttttgctta tccagttcgc taactctaat tcttctttct tccaactttt tgatgcatca
    10081 aaatttcctg ataatgccca catagctgtc ttatctgtcc tgtaattatt caataaaata
    10141 tgtaaattat ttggattttc tttaacaaca gcgtaagtat gtggcgtcaa atctaacgat
    10201 aacgactttg tattattagt atacataggc aacattactt gctgattttt attgatatta
    10261 gcattactat tataaatata ccaggaatta cctacacgct gagcataccc atctccttca
    10321 taaagttttg gataaagcga gtttaaatag ttgactttac tagacaattc ctcactattt
    10381 ttagtcaaaa tttttgcatt agggaatata gatgaaatct tttccttatc aattttctca
    10441 tgtattacag gaagaatatg atatctccca ttattatata aaggcattgt ttcatcattc
    10501 gaataaagtc cttgataaaa tccgtttaat gaactaatcc taccttctcc attccaaaat
    10561 acagcttttg ttctatttac aacttcttcc ttacttggag ctggattttg tatagcatgt
    10621 ctaaagaaag gaataatacc tttagtaaat gctggagttg gtacatcatt tgtcataaat
    10681 gtatacgcgg cacattcgaa attataaact gtgcctcccc cagtatatac attcatcatt
    10741 tccatagcaa tcattgattc tggttccgat gcataggatc tcatatccct agctcttcca
    10801 gtttcaaatg tgtttgtata atgtttttcc caccatttcc atgtatctgt tgatgagccc
    10861 cagttatcac ataagccact caaccaaaat ccactaacga tagaatctgt acccgcatca
    10921 tctcttattg gcgtattttt agttgccaac accaaatttt tatgatattt ttgactcgct
    10981 tcaaagaatg tcggatcatt cataatagtt tcccagaacc atttttcatg atcatgccag
    11041 ataaaatgcg ctccatattt ggcacaaact tccaaatatt tagcactatg cggagctaac
    11101 tggttattgt aaatccaata attctcaata tttaaaacac cttttaacac actatacttt
    11161 tggaattgtt catctaacca ctctggagga actgtattac gctctccagc cgacataata
    11221 accaagaaaa ctggaatgtt tagactttga gcttcctcca acatcttgac ataccattct
    11281 ctcaattctt tcgtatctcg tggaatacaa cttgttggtt tacagacttt tgcaggatga
    11341 agttcaatcg ctgcatatgg ctttacatct tcaggaatag cttcccatgc tcccttcaat
    11401 gtgttccccc accataatcc gttaccatta tcattgccat acaacggaga aagcaacaat
    11461 ggcgattcat tattaatcgt cgttctcatt tgaacacgat tatcagcctt aactcctgct
    11521 aacaatccaa aacataacat cccaatacat acaaacaata ttgtgattaa ttttcttttt
    11581 attttttctt tattcatgac ttatacctaa aaatttaact caataatttt ttctgaatta
    11641 aataccaatt caatattttg tacatcagta tttttgccat atattcttac tcttactttt
    11701 tgatctttgt ttcctccttc caattttagg aatgttatat ccccattttt ccaagcaaac
    11761 gataccttat atcctcctct tactctgaaa cctttcactt ctccttctga ccaagcagaa
    11821 ggtaaagctg gaattagtga taaccaatta tgatggctct gtactaataa ttcacaaatt
    11881 ccactcacca aacctaaatt accatcaatt tgaaatggtg gatggtcaag aaataaattg
    11941 ccaagagtcg cattatttaa caaaccatta atctggttat aagcaggttc accttgatat
    12001 agtctcgcaa aaaaatgaat cagccatgca gcactccaac ctgtttgtgt actagcatgc
    12061 aaaccactta ctaaccaatt attaatcgct tgctccctct cctgtgaaga taaaaaatta
    12121 gcgtttgata atctcctatt gatagtgatt ttagctgctt ctgctaattc cggagtttta
    12181 tgaatatcaa tctcattata aggataaagc ccaaatagag gtgaaatgtg tctatgccca
    12241 ggctctactt cttcataatc ttctaaccat tcttggattt gcccattact acctattttt
    12301 gttttaggta gtttcttttt taactccttc acacgactaa taaaatccga attgtctcct
    12361 aattgttttg caatgccaat gcatgaatca caaaaatatc ttagaatttg attatcaatt
    12421 gtagatgata gacaagcatt tccttcaata ccatttttta agcgatattt attttccggt
    12481 gagacacttg gacctgtcat caagtagcca tccacctcaa ataaataatc ttcaaagaaa
    12541 agaaatgctt cttttatcat ttcaaaatgt tccgtaagaa tacgctcatc ttggaaatat
    12601 aaatagtgtt cccaaatatg agtacataac catggaatag ttaataccca aattgcagcc
    12661 cccatggcat gagattgggg agccgtatcg ccaaaaccat ccgtattatg atgtgctgta
    12721 aaacctctag ctccatacat tttcttagcg gttagtcttc ccggttctct cattctttcg
    12781 agcatatcaa ataatggata ttctacttct ggtaaatcac atggacctac catccaataa
    12841 ttcatttgag tattaatatt aatcgtatat ttagaacccc aaattggatt taattcatca
    12901 caccatattc cttgaagatt ggcaggtaaa ccattcggtt gactagacga tattaacaga
    12961 tatcttccat aatgaaataa caagttagtc aagtagttac tatacttttt agtgttttca
    13021 agaagtagat tcgttggaat gctaagacaa cctttactat agtctagttt aaaatcaact
    13081 ctattaaatt gctcctgata tttttttaca tgttcatctt tttctgtaaa gtaatcaata
    13141 ctactaaatt ctccctgaag agaagaaata tctatatttc cccaataatc cgtcattgat
    13201 ttgagataaa gaaatacctc tgtagcattc cgaataacta ttgtctctcc caatacactt
    13261 acttcaccat ccgtaacctt agaatgacat actactttaa actgaacacc ttttctacct
    13321 ccagcagagg ccgacattaa aattgtactt gaatccagtt tagatacttc gtcattaaac
    13381 cgtttatttc tacccaaatt aatgtttaaa tttaatgtgt tttgaactga tgacactata
    13441 cggcaacata aaatattctt attaaaactc gtaaaatatt ctctttttat ttgtaaatta
    13501 caactattag gctcaaacac aacattagaa atagctgtat ctaaatctag ctctctttca
    13561 tacaatgaaa gagcacaaga ctgaatatct atatgctcaa tgtaaagttc cccaagtaat
    13621 tcatagtggc tttgatctct tggggtagca aacactgtta actttatcaa ttcttcggct
    13681 ttctgaattt ctccatctaa aagatattcc cgaatttttt taagatgcaa tagtgagtct
    13741 ggattatttc tatctgactt tcctctatac caaatagtct catcgtttag ttgaatacat
    13801 tcttttgtag ctgaaccata aatcatacca cctaaatgac cgttcccaat cggcaaggct
    13861 tcattccaat ttgcggcagc tttcttatac cataatttca ctctttacct ctcttccaaa
    13921 atggaggtga tatcccaaat accacctcca cactttctac tacttcaaaa ttcctaaaac
    13981 aactagaata actccaatag ccataacact aaagattagt acaaccggat tctttccttt
    14041 tctaattaat ttacacatta ataaagtcaa agccataggg ataaatcctg gaattaattt
    14101 tgtaatcatt tcttgaacag aaataacaag ttccccctgc ttaaattcta atccaaaatt
    14161 aataccaacc attgatggaa tcaaaccacc cactagtact agccctaatg ctgtcgccat
    14221 actcgtaacg cgattcaatg ttccttttgt attattttct tggataagac tagaaccctt
    14281 agtataccca tatttcaaac cgaaatattt aacaggaata ttaataatat taaacaatat
    14341 taaggcgata aagataccta aagcaccgcc gtctttagaa taagacgcac caatactaaa
    14401 acagatagga actagtgtca gccagaacaa actatctcct agaccagcca gtggccccat
    14461 caagccaact ttaataccag taattgaagc tgcaccttca tttccttctt gttcttccat
    14521 agcggaagta actccaagaa taaatggtgc cgctgtttga tgagtattga aaaactcaag
    14581 gtgacgtttc attgcaggag aagctgaatc tttgtctggg tatagttttt tcaatactgg
    14641 aagaattgta tatagaaaac ctaggttctg cattctctca tagttccatg aagaaccata
    14701 cataaaactt cgtttgaaaa cttttttaag ttcaacttta gttactttat tattcatcat
    14761 agatcaagat cctcctcaac atcactactt gaagttgttt cttgtggttt gcttccaatc
    14821 atatcgtaga agtatgctac cgcaatacca actagtgaga tcccaatggt tgacattcct
    14881 ccatacacag aacaaatgaa tccaatcaac aagaataccc acatattttt cttgagcatc
    14941 atatttaaaa gcataccaaa accaattgaa ggtagtaaag cacctgcaga gttaagtccc
    15001 tgcataacga ttggtggaac cttagcaaac aagtctgcca catattcccc tccaagcata
    15061 atagctagga aaattggtac aattttaaat aacgtgatta aaaccaaact tgaataatga
    15121 taaaaaccga tctttgaata atctccgttt ttagcatcat tatcagcttt tttcacaaaa
    15181 taaacatcta aagtttttgc aagaacatcc aactgttggg tagcaactgc aattggaact
    15241 gctatagcga taccagcagt ttctccttga ccagataaaa taccaaatgc agtaccaata
    15301 atcgcacctg aaatagtatc tggtggagta taacctccaa tacctgttac accgagccaa
    15361 gttaattcaa gagctgaacc aataagaaca ccttgggtga aatctcctaa tactaagcca
    15421 accattgcac ttgtaactaa cggacggtga atcataaata acggtccatt atagtcaata
    15481 gtggcaataa tcccaactaa tgtcaccagt aatgcttgtg tgaatagcat atactgtacc
    15541 tccttaaatt atttttataa aaatttttcc aacattgtaa ctttatcatt aggaaccatt
    15601 tgaatttcaa ctctcgttcc cttatcaact attttcttaa aataatattt gtcttcttca
    15661 cctagagcta cacctcccgt taccttttca ttttcctccg ttttactcat ctgtcctaca
    15721 ttgaggtact ccaattttaa atttccttca atagaatcat acacatcttt tggatttgta
    15781 tatatcagca ttgttctctt ttttattggc acagagttta aaacttccac aaaccgtttt
    15841 acactaaaga aaacaatttt taaacctacc ggtgcagaaa tctttaaaat agattgtcgt
    15901 gttttatctt ctgagatgcg atcattaaca atgataactt gctcaatatc atactttttt
    15961 agccacgtag tgacaacttg accatgtacc agacggtcat caatacgaac aaactctata
    16021 gacataaatc ttcttcctcc tctacgttaa gttgttgttt aacattaaac aaactatttt
    16081 gagcattgtg aacaatttct tctaaattga tttttgaatc ataacttgat attaattcca
    16141 ctaggagagg gatattaaac cctgttacaa tatcaactga atccaaattt aaaaaccgtg
    16201 acaaagccac attattagga cttcctccaa tcaagtcagt caaaacgata acctcttgat
    16261 ttgagtctaa cagttcatcc acttgatttt taaaataatg ctcaaactct acaatattct
    16321 ctccaggcat caaacctaat gtcctaactc tatcatttgt tgtctcacca acaaacattt
    16381 ctgcggtcat gagagctccg ctagcaaaat taccatgact ggcaactaaa attcctctat
    16441 tgaacatttt ctctttcaac aactactcct ttcttaagga taatattagc ataaagtgaa
    16501 gtttctcctg tagcaacaat agcataagct ttcttactac gttcataaaa gtcttctctt
    16561 ctaagataag taatcgtttt aagatctgta ccatgacctt caatcatctg tctataggta
    16621 ccccatatct taggaatatc atcacccgaa acaacgttca taaactgaat tgaactatcg
    16681 acgtaactat ctaatggcat taaatacaga atggaatcta ataattctgg aatatttaca
    16741 ccatcacaac gaattagctt atttgcacat gaggcagaag gataattcgc gtcagctaat
    16801 actatttcat ctccatgtcc catttccatt aaagtcttca ataaatctgg agaaatattt
    16861 ttcggtatat gttttaacat taatatcctc cctattttct ctgaccgtat gttttaaatt
    16921 tttctgccat caattccatc tcctcatcag gaagaactac tggctctcca aaattcttag
    16981 ctaaacaata aatttttgca caatattcaa cttcttcaac aatattaaat gcattcaata
    17041 aattttgtgc acctgctaaa attccatgat tcgctagtaa aactgctcta cgaccttcca
    17101 ttgctttagc tgcattcaca gccaattctt tcgtgccata tgttgcatac tcagctaccc
    17161 gaacatcttt ccctgccact gcaatcatat aatgactcgc tggaagtggt tctctgagac
    17221 aagctaatac tgttgcataa gttgtatgag catggataat tgcatcgata tcatcacgag
    17281 tttgatattg aatcaaatgc atataccatt cgctagatgg caagcgttct ccctctacaa
    17341 catttccatt aatatccatc actacaatat cggattcttt gatttcaaag aaatcaatac
    17401 ccgacggggt aattgccatc aattgttttt cacgatcgaa aacgctgaga ttcccacctg
    17461 ttcctttcgt caaatctgtt tctactagct tcttaccata tttaattaat tcttgtttta
    17521 catctgacat ccttgtcctc ctcatatcaa aacactaaat atttttcaat tcaccaatta
    17581 tccccccttc ctgcacaaaa gctaagaagg gggagggagg caaatttaca aactctcaat
    17641 ctttttaatt cttttgggag taataactct ctaattgtag aaactcctca ataacatggt
    17701 gagcctcttc catccctttt aattgtccca tagctatgag ctgcacaaca atattcccca
    17761 cagctgtagc ctcagtcaaa cctgtaagaa cctctttacc agttctatca gcaatcattt
    17821 ggttaaagta actggctcta gcacctcctc caatcacata tatcctctta taaaccttat
    17881 gagttagttc ttctagtaac tctatcgctt tcctatacgt ttcagctagg ctttcataaa
    17941 caatcttaaa tagttgtcca tctgtccact ctctagtttc atgatgataa gctagatatt
    18001 ctgtcaaagt cttgtgcata tcagattctg ttgcaaattc agttgattca gtatcaatca
    18061 gaggaagatt ttctttttct ttctccacca ttgtcctaat atcatcaaaa gaataggctt
    18121 tccctcttcg ttcaaatgaa cgtcttagtt cctctatgat ccacaaccct gtacaattct
    18181 tcagaaatgt aatcactcca tctttaccga cttcatttgt aaatccataa ctgaaggatt
    18241 cggtagtaag aatcggtgaa gtaagttcca ctccaaccaa agaccaagta cctgatgaaa
    18301 taaataaact accttctgtc ttaggtactg agacaatcgc gcttgctgta tcatgactac
    18361 aaacattcac aacaggaata tcgcctaaac catactcctc ttttatcctt ccaagaacat
    18421 ttccctctga aacaatttca ggaagtaaag atgaatccaa ttcaaatagt tttaagatat
    18481 tctgattcca attttgactc ctaggatcaa ataattgagt tgttgaagca atgctttttt
    18541 ctgtagcaaa cttacctgtc aagagataat taaacaaatc tggcattaaa agaatcttat
    18601 tggtcttata gaaagagtca ggagattctt gacgtgcctt aaagagttga aacaaggtat
    18661 ttatctccat aatctgattt cctgtctctg aatacagttt ttctaattca gtcatttcag
    18721 atatttcctt taacactccc tttgttcttt catcacgata atgaacaggt tgtaataaca
    18781 gcttaccttc attatcaatc agtccaaaat caactcccca tgtgtcaata ccgatagata
    18841 aaatcttgta actagtatta gccaagcgga tactttcaag aattttagct agtagaaagt
    18901 caatatccca agataaatgc ccttttactc taataggtag attagaaaag cgatttattt
    18961 cttccattac tagtttattt tcagaaaggt aaccaacgat tgctcttcca gaagtcgcac
    19021 ctaaatctat tgctaaaacc gcttccattt ttcttctctc ttcgtccttg attaacttta
    19081 ttataatccc cacgttttat tttttcaata tgtgaacgct tacttgaata atgttcaaat
    19141 gttaacccaa tattcataaa aataattcaa attcctccaa tttgtgctat aatatagttg
    19201 aaatgataaa ttaggaggag cttatgataa aagatgaacg tgtacttgaa ttgattgaaa
    19261 ttatcaaaaa gaaaaaaaga attgccgtaa aagagctggc agaaatcact ttctccagca
    19321 caagtacctt acgtcgtgat ttaattttct tagaaaatca aggtcttatc aaaagaaagc
    19381 acggatacgt gaccctgtcc tctatgaaca caattgaact ttctcatcaa atacgtgaag
    19441 gagaaagtac taggcaaaaa agactaatcg ctagtctcgc taaagacttt attcggtctg
    19501 gtatgtgtat ctatctagat tctagtacta ctgtctacga actctgtccc tatctttctg
    19561 aacttgataa tttgattatt tttacaaatg gtttacatac tgcacaaacc ctatctgaaa
    19621 ctgttaaaga tagctccaaa atctttatca catctggcga ggtcaaacat caatcctgtt
    19681 ccgtggtcaa ctatgataag gaaaattctt tattagatca ttttaatatc gatttagcat
    19741 tttgttcagc aagaggtatt gatgaccaat atgtttatga agcttctctc agccaagcta
    19801 tttcaaaaaa gaatattatt gacaaagccc atgaaaccat cttactgatt gatagttcta
    19861 aattttacaa gactggattt tttaaaatta atcccctctc caaatacaca acctttattt
    19921 cagacaccgt gccagatcaa aaattattag acgcagtcga attatttgat ggagaatggg
    19981 tttctgatat tcaatgaaaa tcaaatagga agctagtctc agcttgctca aagtacagat
    20041 ttgaaaattg atcaagtcag ctcgaaacac tgttttgagg ttgtagatag aactgatgaa
    20101 gtcagtcaca tatatacgga aagacgaagc tgacatgatg tgaagagatt ttcgaagagt
    20161 atcgttctgt ctgctgactg atttacaaat attcaccaca tgacattatt ttcttagcaa
    20221 ccctaaaaac agaggctgga caaaaactca atttcaggag aaaatggagt aaaccttccc
    20281 acaataaaac gcatagtatc aagtttttgc acacctgata ttatgcgttt ttctgatttt
    20341 aaagattttt ctcttagatc tgatatttca agaattaagt gaccttgatg atctaaatgt
    20401 attgtggtat actatttttg tcatcggtta ccgattacgt tcctatgggt tcgtgagagg
    20461 aggtgaaact aatgagcctt atcatagagc tcgctcttac tattatagcg gatgttatag
    20521 ctggaattat cttgtatttc gtctgcagat ggctagatag taaggagtag accgactgac
    20581 tagcctataa acacccgtta aatcgctaag atacgtcaaa aaagccctta actacggcca
    20641 tagttagggg ctttggtgtt ctaatgaacc ttatcaatta actacattct agcatataat
    20701 ctacaatagt tcaagcatta agtaaggttg atgacctgaa tgtagtgtga tatactattt
    20761 ttgtcatcgg ttaccgatta cgttccttac ggttcgtgag aggaggtgaa actaatgagc
    20821 cttataccag agcttgctct tactattata gcagatgtta tagctggaat tatcttgtat
    20881 ttcgtctgca aatggctaga tggtaagaag tagaccgaat gactagccta taaacacccg
    20941 ttaaatcgct aagatacgtc aaaaaagccc ttaactatgg cactagttag gggctttggt
    21001 gttctaatga accttataca ctaactacat tctagcatat aagcccagat atttcaagag
    21061 ttttatttat tgtttaaagt tctgaaaggt ctataatgaa gttagccatc tagtatcaaa
    21121 aaaccgacta gctcttatga actagtcgat ttctcatcaa tgcgccaaca tttcttgggc
    21181 gatttcttgg ccagataggt tatctgggta gtaggttggc cagttgtcca tttcttcaaa
    21241 gagggcttct tggcttgtgc ctccaaagaa gatatggaaa tgttctgcct taactggggc
    21301 aacattgtgg tcactaaact gaacatactt gaattgtcca gcgtcagcat ctgtggcttc
    21361 aaagaggaaa cgcacgccac gattgccttt cttgtaagtc aaaattttct taccgacata
    21421 cttgtaagtg tatttcttgc tttgtccacc ttgaacaaat tccatagtat tatcagtaat
    21481 gttaatctta gtcacatctg tatgatagcc ttttgtatag taagccttgt actcagcctg
    21541 ggtcatctta ccagtcaact tagccttgta gtcaaagact tggtcaaacg tgccgtcttc
    21601 aaggaaagga taaactgatt gccagttacc tgcatagtca ctcaaggtgc ggtccttgac
    21661 agctgcatcc tcgaagtaac cattttggac tgtcttggta tcctctgcct tttcaggttc
    21721 aattgctggg ccttcttggt ctgttgtttg tttcaaagcc ttgaggtttt tctccatcac
    21781 ggaaatgtag ttttctccag ccttggtgtc ctcttctgtc agactttcta aaggattgag
    21841 gacatcagtt ttgacacctg cttcttttga aagtgtgtta gcaagggctt gtgaggcatt
    21901 ttcttcaaaa tagatatagg cgattttatt tttcttgaca tactctgtca attctgccaa
    21961 gcgagcagct gatggctctg catctggaga aagtcctgag attgcgactt gtttgagtcc
    22021 atagtccaag gcaagatagt taaaggctgc gtgttgagtc acaaagctct tttgttttgc
    22081 ttgagacaaa ccttctgcgt aagccttatc caaggcttgc aatttttcga tataggcagc
    22141 tgcattcttc tcaaaggtct cttttttatc aggataatct gctgacaagc tgtcgcggat
    22201 gtgctctact agtttaatgg cacgaactgg tgataaccaa acatgggggt caaactcatg
    22261 gtgatgacct tcttctccat ggtcatggtc tccctcttct tcctcgccac ctggcaagag
    22321 caacatatcg cctgtcgcct tgatggtttt cacttttttc ttatccaagg tatctagcaa
    22381 tttaggtacc catgtttcca tgttttcatt ttcataaacg aaggtatctg catcttggat
    22441 tttggcaact gccttggcag atggttcgta ttcatgaggt tctgtcccag caccgattag
    22501 gagttctaca ttagccgtat ctcctgcgac ttgcttggta aattcataga cagggtaaaa
    22561 ggttgtcacg atattgagtt taccatctgc ctgtttttga ttggaacaag ccactaaaaa
    22621 caaggcacat agactggcta gtaataagct aatttttttc acgttcgtct cctatttgat
    22681 aaaacgtctt actaaactga ttagtataaa gacagttaca aaaataatgg taatacttgc
    22741 acttgcaggt gtttctgcat agtaggaaat gtaaagtcct gctaccattc ccaaaaagcc
    22801 aatcgcactg gcaagcagca taaccgattt aaagtttttc cccagacgca ggacaatact
    22861 agctggcaag accataatgg tcgataccag aagagctcct gctgcaggaa tcataagggc
    22921 aatagccacc cctgtcacca tgttaaaaag aatggacatg gtacgaactg gcaagccatc
    22981 cacaaaggcc gtatcttcgt caaaagttaa gatatacata ggacgaagaa agagaaaggt
    23041 caaaatcaaa acaaccgccg caatgacaaa gagggaaatg acctgttctt cactgatagt
    23101 cacgatcgaa ccaaagagat attggtccaa actcattgaa ctcgagcttt tacccttgct
    23161 catgacaatc agagaaacag ccagacctgt tgacatgagg atagctgtcc cgatttccat
    23221 aaagctcttg taaaccgtac ggagatactc cagaaagacc gccgcaatca agacaatggc
    23281 aatagtagaa acagttggag aaatccccaa aaccagacca aaggctacac ctgaaagtga
    23341 gacgtggcta agggtatcac tcatcaaact ctgacgacgc aagatgagga aggttcccaa
    23401 taccggtgag aaaagactca tagcaataac cgccaaaaag gcgcgttgta taaagtcgta
    23461 agataataaa ctaagcatgg cccacctcct ggccattctc atgaacattg aaacaacgcc
    23521 atggcgagtc ttggttacgg actagatgaa tattgcgatc cgcataatcc ttaacttctt
    23581 cagggtcatg ggtaatcatc aaaacagcct tgccatgatg atgggcgctg tggtgcatga
    23641 gttcgtaaaa ttcattttta cttcctgcat ccatccccgt tgtcggctcg tctaggataa
    23701 acacatcagg gtcagaagca aacatacgcg caattaccgc tcgctgcttt tgtcccccag
    23761 atagagaccc caagcgtttg tctcgatgtt cccacatgcc aactgagtcc agactagcct
    23821 tgatatgctc ctcatcatga gcattcaaac gacggaacca gccttttctc ggatagcgac
    23881 ccgacttgac aaattcatag accgtacttg gaaaaccagc attaaaactg gcaatttgtt
    23941 gaggaagata ggctattctc aatttcttac cttgcgtatt tgtctttgaa atagccacct
    24001 ttccaatgcg tggttgcaga attccaagac tagccttgat gagcgtcgtc ttagccgctc
    24061 cattttcccc agtcaaggta acaaattccc cactatcaac acaataattg atatgttcaa
    24121 gaacaggctc cttatcataa tagaaggaca aatcctctac cgtaatatat ctcattattt
    24181 gatttctcct actaaagcag tcaaaaaccg ctgaatcact ttttgttcat ttggagtaaa
    24241 ctgagtcgcc acttgttcat aggttaaaag tgtatgctca tggtgatggt ggtgctcctc
    24301 agcgattgga cgagccaagt cagtcaactg ataaaaaatc acacgcgcat ctttagaatc
    24361 tttagatgtt tccaacatcc cttccttgac caaagactta atggccttgg taactgccgc
    24421 ctgactgaca ttgagacgac gggccaattc tgaatttgtt aaagattcct ctgacaagag
    24481 cataaggata tgctcctgag tattggtcag ggccacctcg ctagtgcaat gacctattag
    24541 gatttcatgc tgattttccg cctgcaaaat cacctcattc aaaaaagcat tgatatcctt
    24601 tgctagctgt ctcatatctg actcctttcc ttttagactt ctctttttta agagaaaaat
    24661 actattcttt gacattttgt ttaccagtta attatatcac aagcaaaaaa agagtcaaga
    24721 aaaaacgtga aaactagttt cattcttgaa ctcttctata ttatattatc tattgaaatt
    24781 ctttgacatc tccatcataa gtcgcccaat ctttgctgaa aaagcgctca ttcagatggt
    24841 aagtcggagc tggtgtggga ttggatagga aaggatcaac tgccttgtca aaagccaacc
    24901 aacccaacca accaaggtga atggtgtcct tcataaagaa aggctccccg ccgtccttag
    24961 aaaaatctgc tatattggta aaaccttgac tttctaactg gtagcgaatc ttctgcaccg
    25021 tttgttggta catatcctct cgtagaccag catagttcat ccatttttta ttaacaggtg
    25081 gaatgataaa aatcgggttt accttagatt tagaaaactg tgttaaaacc aactgcaagt
    25141 cattatactc tggcgacttg agataggtaa agcttttctg agaatccttt aatttcttca
    25201 aatccttctt gatctgctca ttatagaaat aattttccat tcccatctca ttattggaag
    25261 tatttttttc agcatctgct ttgacaacat cttctattgc ctgataagaa aactggtctg
    25321 gcaagatttt taaatactta gctacatgct tatcgtagtt aacatagcct ctaaccgaaa
    25381 actgaccaaa aaaggaagct tggcgttcat taaaacgagc caataattca atcatttcat
    25441 tgtctgctgt cgacaattct tctttacttg ccaacttctg aaccaggtcc ttcatagcta
    25501 cgtttgggaa ctgttgcagt aagcgagtcg ctgcatattg actagcctga tccccagatt
    25561 gatgtttcag aaaactagtc aactggtctc cattaaaata ctgctggaag gctgctggat
    25621 catagccatt tttactgaac cactgaggtg agataacata cacaacttgt ttattctcca
    25681 gctgtggtaa catctgttgc attccaaaat attggttaag cgatgcagct cccccctgtc
    25741 ctaaaagata aggacggtag gaacgattgt atttctcagc taataccgca ggatgagcac
    25801 cgtcaaaacg aagccattca ctagagccaa agaagggaac aaaacgcaca tttggatcag
    25861 atagtgctct gactttttga cttcgctcct taaaactatc gatagtagta gccactgctg
    25921 aacgcttttc agctcctaga ttatgatgca tctcagtagg ataaaagaaa atgagcagaa
    25981 aaaccaacaa accagcgatc aagaccggtc cgaagatcat ccataagcgt ttaagcattt
    26041 tgtagctcca caataccagc tatgatttta ttagctgtat tccagtcgtc acgaccaaac
    26101 tctgttacag ggacacgaat gtcaaaacgg ttctcaatct ccacaatcaa ctcaaccgtt
    26161 cccatactat ccaagacacc tgcatcaaaa agatcttcat ccatcatgtc agaaacatct
    26221 tccataaaca actcatcaat aatttcaata acttctgatt tgatatccat attttatttc
    26281 cttttatttt ttaaaccata gattattcaa gaatccagaa aagattaaga atgacaacat
    26341 gacaacatgg aaagtgacaa ccatgccaag caactgaatc cagcgattct caggtagggc
    26401 agccttccct gcttttttcc gttccttatt gagcgttttt ttcttgcgaa cccaggcatc
    26461 attgatgacc aagcctagtc catgaaagag tccataggcg atatagtacc aggtcacacc
    26521 atgccaaaat cccataatca gcatatttac aatgtaggcc atgcttgagg ttacattacg
    26581 atttttaaag actttctttc tggttaacac catcaccatt cgcataaaga caaagtcacg
    26641 gaaccagaag gacagactca tatgccagcg attccaaaac tcctttaaat cccttgataa
    26701 aaagggcttg ttaaagttga tagggctacg gattcccatc aagtttgaga tggccaaagc
    26761 aaacatagaa taacctgcaa agtcaaagaa gagttccaga ccaaaagtat acataactgc
    26821 caaggcatag agattaaaga agccacctga ctgcaaggct aaattcttca gaggaggtag
    26881 taaggtctct cctaaaacat gagctaggat aaacttatac aaaaagcccc acatgatata
    26941 gcggacagat tcatccagca tatccatcaa ctcatctcgc tcaggaatag cctgataatt
    27001 ttcattaaat cgcttaaagc gatcgattgg accactcgag aaagttggca tgaagagaag
    27061 gaaacggagg aattcccaga gggtaaaatc cttaatcact ccatctctca gctcgatgac
    27121 aattccaacc gaacgaaagg tcaggtaaga aattcccaag aacccaagca aagactgcgt
    27181 tccattgata gctggttgca ccttgacaaa gataatcgga agtagggaca gaaaactaac
    27241 taagtagaag acccacttgc catccttgct ttttcgataa tgcttgtaga aaagcaggag
    27301 caatatttcc cagcaaaggt aaatacccaa ggcagctagt tgattggtct ttccacccac
    27361 caacatggtg acaataaaga agagacttac caacacttca taccaggcaa agcgtttctt
    27421 gaaaaagaga cctataaaga tgggcaaggt tgcagcaatc acataaacaa aatactgagg
    27481 attgccatat ggctctaaat gaggaagctg ttgaaaaaac tccatcatct cttattcacc
    27541 tcgttaatca atcctttgat gtcaatcttt ccatttggag ttagtggcaa actgtctcgg
    27601 taaaggaatt tagatggcat catataggac atcatgatgt ctgtcaggtc ttccttgatg
    27661 gccttggtaa tatcgatatc tcgctcaaac tgctcacgaa caccgtcttt taagatgaca
    27721 taagccaata gattttgtac cttgtggtcc ttgttatagc gcggtactgc gacagcagat
    27781 tcgataaagc gagacttgtt gaggttttga gagacatctt ctaactcaat gcggtaaccg
    27841 ttaaacttaa tctggaagtc catgcgtccg ccgtagagaa gcaagccctc atctgtcatg
    27901 gttcccacat cgcctgtgtg ataggctggc agatcttcaa actcaaagaa ggcttctgct
    27961 gttttttcag gattgttcat ataacctttt gaaacagctg gcccagaaac aatgatttct
    28021 ccctgctcac catttggcag tttatttcct tcctcgtcaa tgataaaggt tggagaatca
    28081 gccttggtat agccgattgg taggcgtttg agagtcgcta acatctcgtc tgtcacggca
    28141 actgctgaca gagctactgt cgcttctgtt gggccgtaag cattgatgat acgggcattt
    28201 gggaaacgct cgcgcagttt ttgagctgtt ttgaccgtca attcttcacc atcaaagtag
    28261 aaatgcgtga ttccaggcat tttctcactg ttgaagtatt cagacaacat ggccatatct
    28321 gcaaaggatg gtgttgatgt ccagatagcg attggcaatg aaaagatagc cgcaaagagt
    28381 tgcttaaagt cctgagtgat gactgaagga agagtgaaaa gcgtaccacc aagtgccaag
    28441 gtcggtgccc aatacatgac agacaagtca aaagaataag gtggctgtgc cagcatttgc
    28501 ggacgactcg gtgtcgcaaa ttccttatcc gtaatcatcc agtttgtaaa gctgaggaga
    28561 ttatcatgtg aaatctgcac tcccttaggc ttaccagtcg taccagaagt aaagataatg
    28621 tagtaattat catctccctt gactggatgc gtgatttcat agttattccc ttgggcaaag
    28681 gcttcttgaa cctgagctag atttatcatt ggtgtagaaa cctgctccaa gggaaaggct
    28741 gaaatggcaa taatcaagct tggctctgct acttctaaaa tagctgaaac tcgctccaag
    28801 gccgaatggc tatcaattgg aatgtaggca tgacctgact tagtcagcgc tacaaagctt
    28861 gccaacattt catattcttg gccaccaaaa acaaccacag gagacttctc aggcaagcct
    28921 agttggtcaa tgactgcagc caaactatcc gaatcagcct ttaaatcgcc ataagtgtgt
    28981 tcctgcccca aaacattata gacaggatag ctaggctgtg tctgagcaaa atgctcaatg
    29041 gtttcaatca tatctgctat tggtttattt gacacaatag ggattctcct tcaagttaaa
    29101 attcattata gataaagctt ccttgaccct gaccaagata gctaaagaag taaagcagcc
    29161 ctagaaagat aagaaaatac aaggctgtcc gaccaagaaa gaggtacaat tcttttctct
    29221 gtttcatcaa gaaaaaccat tcatttctgt aatttttcgc taaaataaga gtgattctta
    29281 ctagcttatt tttctaccat tgtaccactt tatatagtat cttttcaatt gtttaccgta
    29341 tgtttccaat agatttcagc ttattttaag gattatacag tttttctatg tatattttca
    29401 aatagagtga tcctgcttca aaactccatt tcaggagaca atgaagtaaa tcttcccata
    29461 ataaaacaca caatatcaag ttttttcaac acctgatact atgcgctttt ctgattttta
    29521 aagacttttt aaccactctc tcatttaaaa taatctcgtc tgatataaat taaaatagct
    29581 tctatcatca gacaaatggc tgatagccaa aaactgatgc taataccaaa actctcagta
    29641 atatagctca ttagcaaaac aaatactgaa aatgctaatg tagaaatcac ttcaagaacg
    29701 gaatagacat taactaaatg attttcctct actgtttcct gaagaaatac actttcagga
    29761 acttctttta gttgcgataa cataccaact aaagctgaaa ataataaaaa catctgtgcg
    29821 tttggaaaat atagaatagt cagtgtcact atttccatag ctacaagagg aaaaagaata
    29881 ctttcccatt tagcagcaag gaacttttca gatagcctaa aagcaattaa gccactaatt
    29941 ataataccaa tagagtatgc tgtattagaa tatccccagt aactttccgt tttatttaat
    30001 aactccgtta caaaaacaag tataatggaa gaaacccaaa tcgtatttga aaaaatttcc
    30061 aataaatttg ctgatacaaa aagtcttaat ctaggatttc tagcaactaa cttccaacct
    30121 ttgagcaaaa tttcaagatt agtttctgac tctaacacct ccacttcagc gttaggaaga
    30181 aataacatca gaaagctaga aatgatatac aagactaaat tgatacacgt ggtaggtaac
    30241 agaccaattg ttgcaaacaa gagtccacct aatccccaac ctatcaattg aacagcttca
    30301 ccagtcattg ataaggctga attagcctta cccaaatcgg tcgcatagcg tggcacaata
    30361 gcataggaaa cgggtgctgc aaaaccatct agtatggaaa ttgcaacaac aaatagatag
    30421 gtcaccaaag gcgctacgga ttgcattacg gtaaacattc ctaccagtat cgccaataat
    30481 atagtctttc caaattgaga taaagataaa accctattta gcgctaacct tttagtaacc
    30541 aacggaacta aaagactcgc aacaaaagag gatattccta ttaagatagg aactagtgat
    30601 gtggcaatta ctgattttga aataatgtat atgttagcaa tgattgttac tctaaagaaa
    30661 atatctgcta aatttgcaaa taattgagag actaacaact tgacaaatga attagacatg
    30721 tagcgctccc cctttagtta caaactttgg aattaaattt gatttttaat attttagcat
    30781 aaaatagcag aaaacaaaag tgaggttttt tacatgaaaa aacaaaaact attgccttta
    30841 ttattcctaa ttttaggagg aattatgatt atcgttgttg gctagtacta agaagttagt
    30901 tagttagtag aataattaca tcttcaatcg aaaaagagac tggaaaaagt cttaaaaatc
    30961 agaaaaacgc tcaaatcctt tggcgctcta agcttttcta atgatagatt tagatagtca
    31021 aaaaaatatc tgaaaagttc ccaggatcgt cgtctcagca tcaaaaatac gtgaaatttg
    31081 cataattaaa ataaatttca ggggagttaa tcgagtaggg gataatctct agcccctctc
    31141 acaccaccgt acgtgccgtt
//
LOCUS       DAVKNL010000023        31080 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVKNL010000023 DAVKNL010000000
VERSION     DAVKNL010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31080
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.023"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            193..507
                     /locus_tag="VW411_001019"
     CDS             193..507
                     /locus_tag="VW411_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793768.1"
                     /translation="MWVLGFILFMIFFYSNNSKKIKKLENKIKRLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRRVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            517..723
                     /locus_tag="VW411_001020"
     CDS             517..723
                     /locus_tag="VW411_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9793769.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            723..920
                     /locus_tag="VW411_001021"
     CDS             723..920
                     /locus_tag="VW411_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793770.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            976..1656
                     /locus_tag="VW411_001022"
     CDS             976..1656
                     /locus_tag="VW411_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEU9793771.1"
                     /translation="MGMFVGMFKARVESHEIILDVKALMPWISAICLLIGFISMFLTF
                     NFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAFNITGVAIPLAIFISLSEV
                     IILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLEFFATPKDVLNYINSYDEG
                     ERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQLLAFLLVGAIHVYINVMQL
                     PMVKRYFK"
     gene            1668..2309
                     /locus_tag="VW411_001023"
     CDS             1668..2309
                     /locus_tag="VW411_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9793772.1"
                     /translation="MKLLKNLGWILLALLSFLFIYGFIQGLATASLALGASPYAVTLL
                     YVALAGVYVYGIYKWYQKAPVHIEKSGFNRFIWLPVLVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            2327..2557
                     /locus_tag="VW411_001024"
     CDS             2327..2557
                     /locus_tag="VW411_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793773.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            2593..2673
                     /locus_tag="VW411_001025"
                     /pseudo
     CDS             2593..2673
                     /locus_tag="VW411_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            2909..4672
                     /gene="aspS"
                     /locus_tag="VW411_001026"
     CDS             2909..4672
                     /gene="aspS"
                     /locus_tag="VW411_001026"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEU9793774.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITESLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVRGVDFKVFSEAPAVKAIVVTGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALVLEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
     gene            4650..5591
                     /locus_tag="VW411_001027"
     CDS             4650..5591
                     /locus_tag="VW411_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU9793775.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(5667..5747)
                     /locus_tag="VW411_001028"
                     /pseudo
     CDS             complement(5667..5747)
                     /locus_tag="VW411_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(5883..6869)
                     /locus_tag="VW411_001029"
     CDS             complement(5883..6869)
                     /locus_tag="VW411_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9793776.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            complement(6879..7679)
                     /locus_tag="VW411_001030"
     CDS             complement(6879..7679)
                     /locus_tag="VW411_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEU9793777.1"
                     /translation="MLPYPFSYFSSIWGVRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTDKVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASFLLYITKRSRLFSFNTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            complement(7932..8774)
                     /locus_tag="VW411_001031"
     CDS             complement(7932..8774)
                     /locus_tag="VW411_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9793778.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(8776..10068)
                     /locus_tag="VW411_001032"
     CDS             complement(8776..10068)
                     /locus_tag="VW411_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9793779.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            complement(10179..11450)
                     /locus_tag="VW411_001033"
     CDS             complement(10179..11450)
                     /locus_tag="VW411_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9793780.1"
                     /translation="MSSKFMKSAAVLGTATLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIVGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            12000..13517
                     /gene="malQ"
                     /locus_tag="VW411_001034"
     CDS             12000..13517
                     /gene="malQ"
                     /locus_tag="VW411_001034"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEU9793781.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEYFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            13543..15801
                     /gene="glgP"
                     /locus_tag="VW411_001035"
     CDS             13543..15801
                     /gene="glgP"
                     /locus_tag="VW411_001035"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEU9793782.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIDLDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAVVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERFYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(16845..17417)
                     /locus_tag="VW411_001036"
     CDS             complement(16845..17417)
                     /locus_tag="VW411_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793783.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAVWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     FAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
     gene            complement(18216..19064)
                     /locus_tag="VW411_001037"
     CDS             complement(18216..19064)
                     /locus_tag="VW411_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU9793784.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLVPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSKLTEITIEAEI
                     LVGKAF"
     gene            19216..19494
                     /locus_tag="VW411_001038"
     CDS             19216..19494
                     /locus_tag="VW411_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793785.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            19496..21559
                     /locus_tag="VW411_001039"
     CDS             19496..21559
                     /locus_tag="VW411_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU9793786.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWFSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(21637..22893)
                     /locus_tag="VW411_001040"
     CDS             complement(21637..22893)
                     /locus_tag="VW411_001040"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU9793787.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQELDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            23035..25500
                     /locus_tag="VW411_001041"
     CDS             23035..25500
                     /locus_tag="VW411_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU9793788.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     VIFPAILLSIKALFNLLFVLGFLGGMLGAGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAAGGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPEKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            25565..26476
                     /locus_tag="VW411_001042"
     CDS             25565..26476
                     /locus_tag="VW411_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU9793789.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            26706..27404
                     /gene="dapD"
                     /locus_tag="VW411_001043"
     CDS             26706..27404
                     /gene="dapD"
                     /locus_tag="VW411_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEU9793790.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGSVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            27472..28602
                     /locus_tag="VW411_001044"
     CDS             27472..28602
                     /locus_tag="VW411_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEU9793791.1"
                     /translation="MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPDQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            28614..29153
                     /locus_tag="VW411_001045"
     CDS             28614..29153
                     /locus_tag="VW411_001045"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEU9793792.1"
                     /translation="MKSELRKQVLHEMKAISQEQKQAIDQALTERLLQHPFYQEAKVI
                     ATYLSFSHEFQTQELIEQALKDGKKVLIPKTYPKGRMDFVVYDPQQLVKTFFALLELQ
                     GDLEVVDASQIDLIHVPGLAFTTKGYRIGYGGGYYDRYLEHFSGRTLSTIYPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            29137..29814
                     /locus_tag="VW411_001046"
     CDS             29137..29814
                     /locus_tag="VW411_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU9793793.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGL"
     gene            29828..29905
                     /locus_tag="VW411_001047"
                     /pseudo
     CDS             29828..29905
                     /locus_tag="VW411_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(30115..31056)
                     /locus_tag="VW411_001048"
     CDS             complement(30115..31056)
                     /locus_tag="VW411_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793794.1"
                     /translation="MENKFVEFIENNKKVIISSVAVGVVLVLGFGWYSYNQQQAEQQA
                     KIVQLEKDSKSDKEQVDKLFESFDASSDESISKLKELSETSLKTDAGKDYLNNKVKES
                     SKAIVDFHLQKGLAYDVKDSDDKFKDKATLETNVKEITKQIDFIKKVDETFKQENLEE
                     TLKSLNDLVDKYQKQIELLKKEEEKAAEKAAEKAKESSSQSNSSGSASNESYNGSSNS
                     NVDYSSSEQTNGYSNNYGGQDYSGSGDSSTNGGSSEQYSSSNSNSGANNVYRYKGTGA
                     DGYQRYYYKDHNNGDVYDDDGNYLGNFGGGIAEPSQR"
BASE COUNT         9062 a         5671 c         6762 g         9585 t
ORIGIN      
        1 gaagctgacg tggtttgaag agattttaga agagtattaa tgatagataa agtggtcagg
       61 aacctactcc tgaccttttt cttttgcaaa atgacaaaaa tcataatttt tttgacaact
      121 atacttgtca aaaagaaaaa gatatgttac aatgaattca agttaagaaa taggaagcag
      181 aaaggagtta taatgtgggt actaggattt atactattta tgattttctt ttattctaat
      241 aattctaaaa aaatcaagaa actagagaat aaaatcaaaa gacttgagcg aaaagagaaa
      301 ggaaacgcag aaatgtcgag attattacaa gaaatgattg gaaaggaacc aattataacg
      361 ggagtgtata ttgggccaga taactgggaa gttgtggatg ttgatgagga atgggtaaag
      421 ctacgacgtg tagataatac gggaaaagaa aaattcaagt tgcaacgtat tgaggatatc
      481 caaaccgttg aatttgacgg agagtaggtg tgtaacatgc aactaaaaaa tcgtctaaaa
      541 gagcttcgag ctcgcgatgg tctcaatcaa accgacctag ccaaactggc aggggtttcc
      601 agacagacca ttagcctact agaacgggat gagtacaccc catccattat cattgccctg
      661 aaaatatctc aaattttcaa cgaaacagtc gaatcggtat ttcgtttgga ggaggacgag
      721 tgatgaacaa gtataaagtg atttattatg tagtggtcat agctttatta gtcagcgtat
      781 ttctactgat tgggatggac ctaagctggt ttaatcccta tcaaagcgac caatttgttt
      841 gggtctactt tgctctcatc ccagtaattg aatggattga aaagaaatcc aaaaatctag
      901 caagtgaaaa aggagaatga atatgaaaaa gaaacgtaga ttgttatttt tgatgtctat
      961 tgtcttgggg ggttcttggg catgtttgta gggatgttta aggcacgtgt cgaatcccac
     1021 gaaattattt tagatgtaaa agccttgatg ccatggatat cagctatttg tttactgata
     1081 ggtttcatta gtatgttttt gactttcaat ttcttaaaga aaagcagaaa atttcattct
     1141 ttgtatcaag aggaaatgga tgacgatctg aatgaaacct attatgtgca aatgtatcgg
     1201 aatcttgagt ttggaaccat tgcttttaat attacaggtg tagcgattcc attggctatt
     1261 tttatttcat taagtgaggt gattatattg catacaaacc ctcaaacatt tttcctttct
     1321 ttcttactct ttgtggtatt cttagtcgct caaaaatctc tttttaaaac cattgcgatt
     1381 gttcgtcagt ttgatttgga atttttcgct acaccaaagg atgtcttgaa ctatataaat
     1441 tcttatgatg aaggggagcg tcaggctaat ttggaacaga gttttcgaat tttattccaa
     1501 ttacaccaat atgtcttacc agccttatat atttttctta ttatcatttc tttcttgaca
     1561 ggagagattc agttactagc tttcttgctt gtaggagcca tccatgttta tatcaatgtg
     1621 atgcagttac ctatggtaaa acgttatttc aaataaagga gttatctatg aaattactta
     1681 aaaaccttgg ctggattctt ctagcccttc tatccttttt atttatctat ggctttattc
     1741 aggggctcgc gactgcgtcg cttgctttag gcgcttcacc ctatgctgtt actctactct
     1801 atgtggcctt ggctggagta tatgtgtacg gcatttacaa atggtatcag aaggctcctg
     1861 ttcatattga gaagagcggc tttaatcgat ttatttggct tcctgtcttg gtttggttct
     1921 tatctctggt cgttcaattc ttcttgccag atgatccttc agtaaatcag caaatagcga
     1981 cagacttgac cttgtctcaa ccacttttct cattctttgc tgtggttatt tttgctcctt
     2041 tgacggaaga gattgttttt agagggatgt tagcacgcta tctctttcct aagcaggaca
     2101 atagtaaacg aaccctgatt tttcttctgg tatctagtct tctatttgcc ttgattcatt
     2161 ttccaggtga tgtgcaacaa ttttttgtct attttagcct tggttttagt ttgggtttgg
     2221 cttacattag cagaaaaggt ctggtctaca gtatttctct tcacgctttg aataatttag
     2281 tcggcttttt gatgattctc atgctataat agagtcagga ggtcacatga aacgagtaat
     2341 tttattagca gtgattcagg cagtcgttct atttttcatc attggagcgc tagcttatgc
     2401 cttcaaaggc gatttcttct ataactatct agcagttgtc tttgctccta ttgcaggtgt
     2461 actgcgtttt gggacggctt acataacgga aattgtcttg cctcgaaagg cagccgaaat
     2521 cgctgaaaag cgtaaagcag gcaaaaattc aaaataaaag agtccggtga agttcatcgg
     2581 atttttgtat tatactcaat gaaaatcaaa gagcaaacta ggaagctagc cgcaggttgc
     2641 tcaaaacact gttttgaggt tgtggataga actgacgaag tcagctcaaa acatggtttt
     2701 gaggttgtag atgaaactga cgaagtcagt aaccatacat acggtaaggc gacgctgacg
     2761 tggtttgaag agattttcga agagtattag cgctaatttt tagggctttc acctgacttt
     2821 taaagactgg caaacttttc tgatgatttt catgaagagc ctgcgctttt atggtaaaat
     2881 agtaacagaa taaaagagga gagaaataat gaaacgtagt atgtatgctg gtcgtgttcg
     2941 tgaggaacac atcggacaag aaataacctt gaaaggatgg gttggccgtc gtcgtgacct
     3001 tggtggtttg atctttatcg atcttcgtga ccgtgaagga atcatgcagt tggttatcaa
     3061 ccctgaaaaa gtatctgcag aggttatggc aacagctgaa agccttcgta gcgaatttgt
     3121 tattgaggtg actggtcagg tcgctgcgcg tgagcaagcc aatgataagt tgccaactgg
     3181 tgcggttgag ttaaacgtga cagctcttat tgtgcttaac acagctaaga caacaccatt
     3241 tgagattaag gatggcattg aggcaaatga cgatacacgt ttgcgttacc gttaccttga
     3301 ccttcgtcgt ccagaaatgt tggaaaatct taaacttcgt gccaaggtga cccactctat
     3361 ccgcaactac ttggatgagt tggagtttat cgacgtggag acaccattcc tttctaagtc
     3421 aacgcctgaa ggggcgcgtg attatttagt gccgtctcgt gttaataagg ggcattttta
     3481 cgctcttcct caaagtccac aaatcacgaa acagctcttg atgaatgctg gttttgaccg
     3541 ttactaccaa atcgttaaat gtttccgtga cgaggacttg cgtggagacc gccagcctga
     3601 atttactcag gtcgacttgg aaacgtcctt ccttactgag caagaaatcc aagatattac
     3661 agaaagcttg atcgcgcgcg tgatgaagga aacaaaaggc atcgaagtaa cgctaccatt
     3721 ccctcgtatg aaatacgatg atgctatggc tctttacggt tctgacaagc cagatactcg
     3781 ttttgacatg ttgcttcagg acttgacaga agtggtccga ggtgtagact ttaaagtctt
     3841 ttcagaagca cctgctgtaa aagcgattgt ggtcacagga gctgcggaca actattcacg
     3901 taaagacatc gacaagatga cggaagtagc caaacagtat ggtgccaaag gtcttgcttg
     3961 ggtcaaggtg gttgatggag aattaaacgg accagttgcc aagttcttga ctggtatcca
     4021 agaagaattg acaacagcgc ttgttcttga agataaggac ttggttctct ttgtggcgga
     4081 tacgcttgaa gtggctaatg caacactggg tgcccttcgt ggacgtattg ctaaagagct
     4141 tggcttgatt gataatgata agttcaactt cctttgggtg gttgactggc cgatgtttga
     4201 atggtctgaa gaagaaggcc gctacatgag cgcccaccat cctttcacac ttccacagga
     4261 agagactgct catgaattag aaggtgattt ggctaaggtt cgtgccattg cttacgatat
     4321 cgtcttgaac ggttatgagc ttggtggtgg tagccttcgt atcaaccaaa aagaccttca
     4381 agaacgcatg ttcaaggctc ttggtttctc aactgaagaa gccaatgacc agtttggttt
     4441 ccttcttgaa gccatggact atggtttccc accacacggt ggtttggcta tcgggcttga
     4501 ccgttttgtc atgttgcttg ctggagaaga aaatatccgt gaagtcattg cctttcctaa
     4561 gaacaacaag gcaactgacc caatgacaca agctccatca acagtcgctc tcaaacaact
     4621 agaggaactc agcttacaag tagaagaaga tgaaacaaac aaaacgaatt aagcggtggc
     4681 gctattatct gcgccgcttt gcttatcaga taaaaatttt acgtgtctta caaagtatct
     4741 ctcgagaaaa gtatgatgag aagatttcgg cctctctggt ctatggtttt ttatcagcag
     4801 tagcagttaa tttctttttc caaccagggc atgtgtattc gagtggtgca acaggtctgg
     4861 cacagattat ctctgccttg agtaatcact ggtttggttt tcatattccg atttcgctaa
     4921 gcttctacgc cattaacttc cctttgatgg tcttagcttg gtatcagatt ggccataagt
     4981 tcaccgtctt tacctttatc acggtatcta tgagttcctt ctttatccag tttgtccctg
     5041 tggcaacctt gacagaggat cccattatca attccctttt tgggggtgtt gttatgggtt
     5101 tggggattgg ttttgctctt cgaaacaata tctccagtgg tgggacggat atcgtcagcc
     5161 tgactattcg taagaaaacg ggtaagaatg tcggtagtat ttctttcttg gtaaatggaa
     5221 ctatcatgct gatagcaggt ttgacctttg gttggaaata cgctctttat tctatgatta
     5281 ccatctttgt ctctagccgt gtgacagacg cagtctttac taagcaaaag cgtatgcagg
     5341 ccatgattgt gacaaatcat ccagagaagg taattgaaaa aatccataaa aaattgcacc
     5401 gcggagcaac catgatccac gatgcagaag gaacctataa tcacgagaga aaggcagttt
     5461 taatcactgt cattacacgt gcagagttta atgaatttaa acagattatg acacaagtgg
     5521 atccaagctc ctttgtctct gtctcggaaa atgttcatat tctaggaaga tttgttgaga
     5581 tagataatta gtgattaata ctcttcgaaa atctcttcaa accacgtcag cttcaccttg
     5641 ccgtaggtat ggttactgac ttcgtcagtt tcatctacaa cctcaaaaca gtgttttgag
     5701 caacctgcgg ctagcttcct agtttgcact ttgattttca ttgagtataa aaagatcttg
     5761 ggaaaagact caatttcagt agaaaaggaa gtaaatcttc ccacaataaa acgcatagta
     5821 tcaagttttt tcaatacctg atagtatgcg tttttgcttt taacgacttt ttgcccagtc
     5881 tcttattttt cgataatttt gtgggcgatc aattgacggt aacaaatttg tttattgttt
     5941 ttattatcat taataatctg gagaatagtt tcaagggaaa cacgaccaag ctctaaacta
     6001 ttgatatcga cataggctgc caagttgagc ttgggattaa ccgagtcaaa gctgagaaca
     6061 gggacatcca gctggtgttt ggcaatatag ttacaaacac cttcagctag gaggctatcg
     6121 gttgtgatga tagcatcaat ttgtggatcg tgcttgaata atcgcttgct aaatttatag
     6181 cccttttctt ccagaaactc gtcggcaaag tagatgcgat tgttgtcagt ggtaagtttg
     6241 taatgtttaa gcgcctgttc atagcctgtt aaacggtctt tggtcacgaa gagcttttta
     6301 cttcctccga taaaggcaat gcgtttgcag ccttttttga tgaaatattc agtcgcatca
     6361 aaaccagctt gaacattgtc gttgtcgaca agtgggatga aaggagatag agatttacct
     6421 aagataagga aggggaactg ttcttctgcg acgagtttta cgagagggtc ttcttcttgg
     6481 gcatagagaa aaattagccc atctacacgc ttgccgtaga ccatttgtga aatagcgttg
     6541 agacgctcct tctcatcttt ccctgttgct atctgaatgg catagtggtt ttcagatgcg
     6601 acttgagaga tgccacgtag aaccgatgga aagaaaggat tctggtagaa ggcgtctgag
     6661 tcatcaggaa gaactaatcc gataacctga gtatagctgc ttaccaagct acgagcgttg
     6721 aggtttgggt ggtagttgag ttccttcata gctttgcgaa cacgtttttt tgtttcgtcg
     6781 ctaatggttg atttattttg aataacacgg gttacggttg aaggcgaaac accagcagcc
     6841 ttggccacgt ctttaatcgt aacgggcata aaaatctcct atttatggta atttggatca
     6901 cggaaatgtg ttttataaaa gatagtgacc agatacagaa caaaaagaat attttgtaca
     6961 gtaatcaggg ttgtcatatt ttggccaaat aatcccaaaa taagtgtaat cagagtcggc
     7021 aatcctaaac agttcaagat aaaatggtag cactctttaa aggtattaaa tgaaaagagg
     7081 cgtgatcttt tggtgatata aaggagaaag ctagctccaa gagagacgat aaagaaattc
     7141 aaaccaaaga ggaagctcgc accgagaact aggaagaggc tgatatagac acgattttgt
     7201 tggtaccagt ctttagaaat tgcttgggtc aagctgtctt tgcttttgaa actctcagtc
     7261 tgaatggctc ggtaagagat gcgggtcagt tccttgcttt ccttgctgat gactagctca
     7321 tttgtatcga aatgcagttg caagtcctta ggtaattcct tgatttgact tggaccaatc
     7381 acaacagaag gggcttgact agctgttcca gtataagtta atgtgccatc gacaattgta
     7441 gcatgttcag agagatcctg gacaacctta tctgtcagag gttcatagac attatcgata
     7501 aaagtttcta gcggataggt ctcctgggag ctgttttgga tagcaatggg taccatagac
     7561 aagctgataa ggaagatact ggtaaagaga agttgaaacc agttgagctc gaaacgtttg
     7621 gacaggggct tacgaacccc ccaaatactt gaaaaatagg aaaatggata tggaagcata
     7681 ggtatctttc taaaaggtgt tttctatatg agtattatta tatagagaaa tgcgctttat
     7741 ttcaagtcta ggagaaaaat tgcttgctac aaggatatta tttacagtga caattgctaa
     7801 taatcaatga aaatcaaagt gcaaactagc cacaggttgc tcaaagcaca actttgaggt
     7861 tggagataaa gctgacgtgg tttgaagaga ttttcgaaga gtataaaatg aaaaagggtg
     7921 gcggggataa attatccctt gtcgccacca cttgtaagtc ctgaaacaaa gttcttttgt
     7981 aggaagaaga agagaataca gattggaagg gcgatgagga tagcacctgc tgagaagtag
     8041 gcaatcttca agtttttcgc attgttaacg aaggtttgga gacctacggc aacagtaaag
     8101 tattctttct cacgaagcaa gaaactagag aggatgtagt ccccgaaagg tcccatgaag
     8161 gcccagagag cttgtacggc aaccattggg cgaacaagtg gtagaacaat ttgccagaag
     8221 cggcggaagt gtcctgcacc gtctagtttt gcagattcgt ctaaagacat tggcactgta
     8281 tcgaagtagc ctttcatgag ccaagcattc atcgggatac caccaccaac gtagaggaag
     8341 atgaggaacc agttgtggtt aagggcgttc aacataagcg ccataacgaa gaaggctgtc
     8401 aaagcggcca tagttggcac catttggatg atcaagaaga agaccaaact ttgtttacga
     8461 gccaagaagt tgtaacggct gtaagcataa ccagcaagta cgatgatact tgtttgaaca
     8521 gccatggtaa ttaaggcgat aatcaaagtg ttgaggtacc aagtaccgta caaggtttca
     8581 gtgaagaggc ctttaaagtt atcaaaattg aggtcgatat tagtatctag tttaaaggct
     8641 gagacgttac ctgctttaaa ggctgacata atggtaatca acagtggata gataattaca
     8701 attgatagac caatcaggta aaggtaagta aggctttgag tcagtctacg tttgagttta
     8761 attgagttat tcatcttaga cgtcctccat atcaaatgcg tgtagtttct tgaatgcgat
     8821 catagagatt gagatgacaa tgatagagat aatcaaggta acagctgccg ccattgagta
     8881 ttgaggagat gtacctgttg tcaaacggta gatccatgag atcaagatat cggttgaacc
     8941 agctccacct ccgacactac caggtcctcc accattgaag aggtacatga tagagaagtt
     9001 gttaaagttg aaggtgtatt ggctaatcaa agtaggtgcc gcaacagcca aaatcattgg
     9061 gaaagtgatg ttgcggaatt tttgccaagc gttggcaccg tcaatataag ctgcttcgta
     9121 aaggtcgtta ggaatagatt gcaagatacc caaggtcaga acgtagatgt atgggaatcc
     9181 gagccaacct tgcatcataa tcaaggcaat cttagtccaa gttgggtctg ttttccaagg
     9241 aataagagct ccatcaagga aaggaaggaa tttagccaag attggcaata cttgagtgtt
     9301 gatagcaccg acactatcgt taaacatgtt tgagaatgtc aagatagtga tgaaggctgg
     9361 gacagcccaa ggaagaagga aaataacacc aaagatacgt tttcctttga taaatggttg
     9421 gttggcaatg atagctgtga agataccaat tacgatttgt aaagtagaag ctgccaaagc
     9481 ccaaatgata gtccaagaaa gaacagaacc aaaggcagaa cggaaggtac tcaagctcca
     9541 aatgtttgta aagttggtca aaccaaccca gtccaacaac ttgtttggtg gcaagtgttg
     9601 gaagtcgtag ttggtaaagg cgatcatcaa ggttacgata actgggaaga taatcgcgaa
     9661 ggtcatggca acataagatg gaatgatcaa gaggtaaggg aagccatttt cataaatccc
     9721 tttgatcatg tctttgagtg tgcgtggaac tggaattcca ttgttaatgc gttttgcaat
     9781 cgtatgtgca tctttgatat ttgagaaata aaagagtaca taaacgatta caaagattag
     9841 atggaaggca ccacgaatca gcataaagag ggaattatca cgacctggtt tgtcaccaag
     9901 agtgatgagg ttgctcaatt caggggttgc aagtgctagg aagtaaagga caaatacgat
     9961 ggttacacca aggaagataa aacctttggc tttttgttta ttgtaaatct gtcctaaccc
    10021 aggaatgata gacagcaggg ctgctttact aggttgttgc ttttccatat taactccttt
    10081 cataggatac tactttttta taagaaagct aaattatttg tgtctctatt gataaattca
    10141 aagggggatt tgatttccac cccccttgaa caaatttttt attcaccaaa tttttgtttg
    10201 attgtttctt tgatcaatgt tacagcatcg ttagcagctg ttttagcatc tttttgacca
    10261 cttacagcat caaagagcat atttttcgct ggatcccaaa ctgcagacat ttgagagatg
    10321 tttggcagtg gttgagtgtt cttgaactgt ttgataacag ctgttgtcaa ctcatcgttt
    10381 ttaccttcag cgtatgaacg agcctcagta ttagctggga tttcgttagt cttatcatat
    10441 aatacttttt gttgttcagt tgcaacaagg aagtctacaa atttttgaga agcttcaagg
    10501 ttcttaacgg cttgaggaat gacccaagct ttaccaccac cgaatgcagc atattctttt
    10561 ccatttggaa gagttgggat agttgcaact ccgtagttta ctttagcatc tttaaaggct
    10621 tgagctttcc aaggtccgtc gatgatagca gctgttttac cttcttggaa ttgagtttgg
    10681 attaagtttc cagcaccttc tgtatcttgc atacctttag gccatttttc gtaccaagat
    10741 ttagcgtagt tgatacctac gatagaaccg tcgtttgcaa gaccgatgtc tttagcgtct
    10801 ttaccgtttt ggccaaagac gtaagcaccg ttaccggcaa gaagtccata tgtatagtag
    10861 aagtttgtcc agtcagctag gaaggcagta gttttaccat cttcaccagc gaatgcgtat
    10921 ttgctatctt tagcaaggtt ttccaagtca gcaaatgttt ttggagcatc tttcaccaag
    10981 tctttgttgt agtacataac aagtgactcg ataacggcag gagcaccgta aactttacca
    11041 ttagcagctg ttacaagaga tttagttgtg tcgtctgttt tagcaccatc gctcaatttc
    11101 acttctgaaa gttgtccgtc agaaccaagg ctacctacac ggtcgtatgg agccatcata
    11161 acatcaggga cattaccaga ttggttgtca agagaaagtt tatcaagacc tcctagagca
    11221 tcaccagttt taagagtgac ttttactcca gcttcttttt cataagcttt agcaacctct
    11281 tcaatatagc tcttatatcc ctcgtctaca tatacagtga gttctttgac ttcagatgaa
    11341 ccagaatcag caggcttatc agcagttttg cttccgcaag ctaccaaaag caagctagca
    11401 agtgtagcag ttccaagcac cgcagcgctc ttcataaatt tagatgacat agtgtattcc
    11461 tcctaaagaa tagcaagttt tattgataag gaaacgcaaa cgttttcctt tatgagctta
    11521 gtatagcaca aatagaaaac ggttgcaagt attttttgta aaaattttaa aaaattttta
    11581 agcttgattt gatagcataa ttttgcattt taatagaaga aaagtatgaa atagataaga
    11641 atacagtatt tttaataaaa atatatgaaa tctgtctaaa gagggaaatc aggaggggct
    11701 accttcctat ttctaatcta tatctatttt ctatagacgt ttgtgctttg ttgcatataa
    11761 attcaagctc cctaaggagg ttttgtactt tgtatactgt ctcgtgagag tcaaacttta
    11821 ggattagata gttttaagtt atctttgcta aaatgagtta gtctccttaa aaataatcaa
    11881 aaaagttttt taaaaacgct tgcaattatg cttgaaaagg agtatactta taagtaacgc
    11941 aaacgtttgc gtctgaaaaa tacgcaacgt tccattattt taacacacga ggtgctatta
    12001 tgaaaaaacg tcaaagtggt gtgttgatgc acatctcttc tcttccagga gcttacggaa
    12061 tcggatcatt tggtcaaagt gcttacgact tcgttgattt cttggtccgt acaaaacaac
    12121 gttactggca aatccttcca ttaggagcaa ctagttacgg ggattctcct taccaatctt
    12181 tctcagcctt cgcaggaaac actcatttta tcgatttaga tatcttggtg gagcaaggtt
    12241 tgttggaagc aagtgacctt gaaggagttg actttggtag cgatgcgtct gaagttgact
    12301 atgctaaaat ctactatgca cgtcgtcctc ttttagaaaa agcggtgaaa cgtttctttg
    12361 aagtcggaga tgttaaagat tttgagaaat ttgctcaaga caaccaatca tggcttgagc
    12421 tctttgctga gtatatggct atcaaagagt attttgacaa tcttgcttgg actgaatggc
    12481 cagatgcaga tgctcgtgct cgtaaagctt cagcacttga aagctatcgt gagcaattgg
    12541 cagacaagtt ggtttaccac cgtgtgactc aatacttctt cttccaacaa tggttgaaat
    12601 tgaaagctta cgctaacgac aaccacatcg aaatcgttgg ggacatgcca atctacgtag
    12661 cggaagattc aagtgatatg tgggcaaatc cacatctctt caaaacagat gtcaatggta
    12721 aggctacttg tatcgcagga tgcccaccag atgagttttc tgtaactggt cagctttggg
    12781 gtaatccaat ctatgactgg gaagcaatgg acaaagacgg ctacaaatgg tggattgaac
    12841 gcttgcgtga aagcttcaaa atctacgata tcgttcgtat cgaccacttc cgtggcttcg
    12901 aatcttactg ggaaatccct gctggttccg atacagcagc acctggtgag tgggtgaaag
    12961 gtccaggtta caagcttttt gcagccgtta aggaagaact tggtgagcta aacatcatcg
    13021 cagaagacct tggcttcatg acagatgaag tgatcgaatt gcgtgaacgt actggcttcc
    13081 caggaatgaa gattcttcaa tttgccttca acccagaaga cgaaagcatt gatagcccac
    13141 acttggcacc tgctaactca gttatgtaca caggaacaca cgataacaat acggttcttg
    13201 gttggtaccg taatgagatt gatgatgcga ctcgtgagta catggctcgt tacacgaacc
    13261 gtaaagaata cgaaacagtg gtacacgcta tgcttcgtac agtattttca tcagttagct
    13321 ttatggcaat tgcaactatg caagatttac tagaattgga tgaggcagct cgtatgaact
    13381 tcccatctac ccttggtgga aactggtctt ggcgtatgac tgaagatcaa ttgacaccag
    13441 ctgtcgagga aggtttgctt gacttgacaa caatttatcg ccgaattaat gaaaatttgg
    13501 tagatttaaa gaaataagac aataatcagg agacaactaa acatgttatc actacaagaa
    13561 tttgtacaaa atcgttacaa taaaaccatt gcagaatgta gcaatgaaga gctttacctt
    13621 gctcttctta actacagcaa gcttgcaagc agccaaaaac cagtcaacac tggtaagaaa
    13681 aaagtttact acatctcagc tgagttcttg attggtaaac tcttgtcaaa caacttgatt
    13741 aaccttggtc tttacgacga tgttaaaaaa gaacttgcag ctgcaggtaa agacttgatc
    13801 gaagttgaag aagttgaatt ggaaccatct cttggtaatg gtggtttggg acgtttggct
    13861 gcctgcttta tcgactcaat tgctactctt ggtttgaatg gtgacggtgt tggtcttaac
    13921 taccactttg gtcttttcca acaagttctt aaaaacaacc aacaagaaac aattccaaat
    13981 gcatggttga cagagcaaaa ctggttggtt cgctcaagcc gtagctacca agtaccattt
    14041 gcagacttta ctttgacatc aactctttac gatattgatg ttactggtta tgaaacagcg
    14101 actaaaaacc gcttgcgttt gtttgacttg gattcagttg attcttctat tattaaagat
    14161 ggtatcaact ttgacaagac agatatcgct cgcaacttaa ctctcttcct ttacccagat
    14221 gatagtgacc gtcaaggtga attgctccgt atcttccaac aatacttcat ggtttcaaac
    14281 ggtgcgcaat tgatcatcga cgaagcaatc gaaaaaggaa gcaacttgca tgaccttgct
    14341 gactacgcag ttgtccaaat caacgatact cacccatcaa tggtgattcc tgaattgatt
    14401 cgtcttttga ctgcacgtgg tatcgatctt gacgaagcaa tctcaattgt tcgtagcatg
    14461 actgcctaca ctaaccacac aatccttgct gaagcgcttg aaaaatggcc tcttgaattc
    14521 ttgcaagaag tggttcctca cttggtacca atcatcgaag aattggaccg tcgtgtgaag
    14581 gcagagtaca aagatccagc tgttcaaatc atcgatgaga gcggacgtgt tcacatggct
    14641 cacatggata tccactacgg atacagtgtt aacggggttg cagcactcca tactgaaatc
    14701 ttgaaaaatt ctgagttgaa agccttctac gacctttacc cagaaaagtt caacaacaaa
    14761 acaaacggta tcactttccg tcgttggctt atgcatgcta acccaagatt gtctcactac
    14821 ttggatgaga ttcttggaga tggttggcac catgaagcag atgagcttga aaaacttttg
    14881 tcttatgaag acaaagcagt tgtcaaagaa aaattggaaa gcatcaaggc tcacaacaaa
    14941 cgtaaattgg ctcgtcactt gaaagaacac caaggtgtgg aaatcaatcc aaattctatc
    15001 tttgatatcc aaatcaaacg tcttcacgag tacaaacgcc aacaaatgaa cgctttgtac
    15061 gtgatccaca aataccttga catcaaagct ggtaacatcc ctgctcgtcc aatcacaatc
    15121 ttctttggtg gtaaagcagc tccagcctac acaatcgctc aagacattat ccatttaatc
    15181 ctttgcatgt cagaagttat tgctaacgat ccagcagtag ctccacactt gcaagtagtt
    15241 atggttgaaa actacaacgt tactgcagca agtttcctta tcccagcatg tgatatctca
    15301 gaacaaatct cacttgcttc taaagaagct tcaggtactg gtaacatgaa attcatgttg
    15361 aacggagctt tgacacttgg tactatggac ggtgctaacg tggaaatcgc tgagttggtt
    15421 ggagaagaaa acatctacat cttcggtgaa gattcagaaa ctgttatcga cctttacgca
    15481 aaagcagctt acaaatcaag cgaattctac gctcgtgaag ctatcaaacc attggttgac
    15541 ttcatcgtta gtgatgcagt tcttgcagct ggaaacaaag agcgcttgga acgtttttac
    15601 aatgaattga tcaacaaaga ctggttcatg actcttcttg atttggaaga ctacatcaaa
    15661 gtcaaagagc aaatgcttgc tgactacgaa gaccgtgacg catggttgga taaagtcatc
    15721 gttaacattt ctaaagcagg attcttctca tctgaccgta caatcgctca gtataacgaa
    15781 gacatctggc acttgaacta atactcttcg aaaatctctt caaaccacgt cagctttatc
    15841 tgcaacctca aagcagtgct ttgagcaacc tgcggctagc ttcctagttt gctctttgat
    15901 tttcattgag tataagatac aaatttatac taatacattt tgtaaaaaag cgagtttcga
    15961 ttgaaattcg cttttttaat gatgtagatt tgggtcaatc ttgtctaaaa atagggaaat
    16021 cctagataca gtgaaggctt taaatgctgg tttttactgt cctcagcctt atattttttc
    16081 gtagttggtt acctcatatc tattatattc gcttacataa agtattataa tataattgta
    16141 ggaaagaagg tgtttttatg atatacacac ttaaattggt gttgtttatt acctttcttg
    16201 taataagctt gttacctgat aagatttttg gaaaaaataa aaaaatttgg aaaatagttt
    16261 ttgcaatatt gacggcagtg gcagcattgt catttatgta ctaagttatt ttaagaatgt
    16321 agggaaataa accctacatt ctttttagtt ttttctgttt tctaaattct atttatccaa
    16381 gcgattcaac atttcttgct tcttcgcttc aagttctgca cgcttttctt cgatttcggc
    16441 atgttttttc tcgagttcag aacaacttgc accattgcta aattcttttc gccatcagga
    16501 gatagggtga gtcgacatgt ctattactca cccaaagcag tcctacaaag caggaatttt
    16561 ctgttacttt tttggaaata gtaacgttta tacagctttg acacttcgta tcaaagcgcc
    16621 aaacacactc cgaggggttt acagaaagca gaaaaggaat gatctggtat aagatcattc
    16681 cttttctctc tttttcttta agtaattata tacaatgtac gacgaagtcg tcattgcaat
    16741 gctgatccac cacctaaagg gaactttaaa caacattgat aagataaaga atataaacaa
    16801 cgaaaatacg ttatacccaa ttaattttat tgtatatctc atgattaaaa gttaatcctt
    16861 ccgttgttag gaatggcatc atttttatcc cataattgtg ctaaataagt ccccggtgat
    16921 aataaattca tagcgaattc taaagcaaca tcatttacaa accaactacc tagatatcta
    16981 gaaattgctg aacgaatagc actttttgct gcatgttttc cttttacttt aattagattt
    17041 gcaaggcctg cagtagttcc tcctaatgct aaagctattg cagtatctaa tagagcaccc
    17101 atttgattaa ctgtaatacc ttgccaaact gctctaaatg gagagtatgt aggtgggatt
    17161 gtataatcgc cttgtaattg tcggttaatt acttctttga tccattgttg tgagacgtct
    17221 ggatgaaaag attggatttc gtttgcaagt gtattgattt gttcttctgt tagagaagtg
    17281 acaggttgaa gttccatatt tgtttcaatt tgtgatactt gttcagaagc gtatacagct
    17341 gaaacacttg gaatcgctga tacaattaac acaattgacg tcaaaaaaac cgaaataaat
    17401 ttcattaatt tgttcatgag cttttctcct ttttatttgc atctgcttac attttatcat
    17461 atactgttat tatagtcaaa aaaatatgct attatgttaa aaaaatattt ttcaaaatat
    17521 aaatggacgg atttattttg gattttattt gttattttga cctgcctcta tattggtaac
    17581 catgatttgt ttactctcaa tcatcaagaa ttctcttttc gtggtagcgt ttggggtctg
    17641 gtactggcct tatatcactt actattcatt gataagtttg ttatatcgaa tcgaaaataa
    17701 agattagagc tatgcttgac tgtgtacttt taggatttat tttggaggaa gattttgtct
    17761 ctattattta ttattttaaa tttatttatt ttgtataaga tctattcttt gaggcgggaa
    17821 aaatcgaaat acttgattta tacggcctat atcatatttg gggtaaatgt agtatatggt
    17881 attcaatggt tattaaaaga actgatttca actatttccc cttaatgtgt atggaaacta
    17941 taacaaaacg cataagatta aggtttgtgt acctgttctt atacgttttt gtcatattga
    18001 agattttcgg taagttttct acttttgcga agtctagttt atactcaatg aaaatcaaag
    18061 agtaaactag ccgtaggttg ctcaaagtac agctttgagg ttgcagataa aactgacgaa
    18121 gtcagctcaa aacatggttt tgaggttgta gatgaaactg acgaagtcag taaccatacg
    18181 tacggcaagg cgacgttgac gtggtttgaa gagtattaga atgctttccc aaccaaaatc
    18241 tctgcttcga tggtaatctc tgtcagtttg ctccagtcaa ttttgctctg gtcaacgtga
    18301 aagaggagag gagtcatact gagtagggct tggagttgtt ctgctgtgat agtcttagtc
    18361 agagaggcag tttgactaga taggatggta aagtgttcct ggaaatgctc cttgatatct
    18421 tggttagaat actccttgtt tgtcagctgg tcctgtactc tttgacggat ttctttgagg
    18481 tgattttcag ttgggataac ctttatcaag ataccgtctt tggataaaac gcgacgaaat
    18541 tctccatagt tggcaggtga gaagatatca agcagaatat ccatgttagc gtcttttata
    18601 ggaagtcgtg ccaagtcgcc aacgaaccaa ttgactgccc agttgggttc acttttagca
    18661 gcgatttgga ctgaatcttt ggagatgtca aaggcataga aagttttttc agagtgactt
    18721 tcttgtagtt tgcgagaata gaatccttca ccacaaccga tatccaaaat tgttgtggta
    18781 gtttttgagc ttgcaagcaa gtcagataca gcatctaaga tagcttggta aaagccggct
    18841 tctaggattt gttgacggtt ttgaaaattt tccttgtcgt agttagcaga ttgcttgatt
    18901 tgagggacta gattgacata gccaaatttc gccaagtcaa aagaatgacg gttgcagcac
    18961 ttgaaattag tctctaacag agtcagattt tcttgacaga taggacaggc aaaggcagtc
    19021 gcagaagcaa aacgctgaag tttgggcttg agatttgtat tcatagttta agtgtatcaa
    19081 aagaggcaaa tgaaagcaac tttaggaata agtagatggg agattcagta gaaataaaac
    19141 taattctcca atttatagaa tgaaattgct tttatatcta aattgctata taataatgat
    19201 aaggaggaaa taagtatgtt aaaagaagta ttaaccgttg caaaagttgc gaaaaaatct
    19261 tcattatttt tgggtggtgt cgcatttggt acccttggtt tgaaaatctt agcaagtaag
    19321 gaagctaaaa aaggttattc taaagctttg gctaaggctt acaagttgaa agacgagcta
    19381 gatgcatctg tttctgttgt gaagcaacat ggagacgatg tcttgcaaga tgccaaatat
    19441 ttgtacgagc aagagaaaaa agaagagcaa ttagatagcc ttataggtga ataatatgtc
    19501 ttttaaagtg ctacatagag gataccaaca tatccgacta tcatcttctt tttcacttac
    19561 cttggatatt caagactatc ttcgttcctt ggcgagagat gaaaagggga ttgagtctat
    19621 ccagttttac atggatcaac agcactttac tctacgcata aaagaaggct tttctgtatt
    19681 agataatgca gaagcctttt taaaaagaat tgataaaggg aaagtttctg agttgatgac
    19741 tcttcccatt cgtagagaag agagtgctta ttctattgtt tcaggtgcag cggttaagcg
    19801 tgtacttttt cgtagttttg tgccgtatcc tattcgctat atatggactt gttatcaggc
    19861 tttgggttat attagagaag cctatcaaac actagcgcgt aaggaactaa cgatggaagt
    19921 cttggactgt tcggctattt tattgtcttt gtttatgaac caatccaaga cagctagcaa
    19981 tatcatgttt atgcttgatt tggggaatca tttagatcag tggtccttga aaaaaactgc
    20041 aacagattta gaacagagtc ttcttgcaaa agagagcgat gtattcctag tacagggcga
    20101 tacggttgtt agtatcaaga gttccgatgt tcaaatagga gatgtcttga tcttatctca
    20161 aggaaatgaa attctgtttg atggacaagt agtttcaggt ttaggtatgg tcaacgaaag
    20221 ttccttgaca ggagagagtt ttccagttga aaaaagagag tctgatttgg tttgtgcaaa
    20281 tacagtatta gaaactggag agttacgcat tcgtgtaaca gataatcaga tgaacagccg
    20341 tattttacag ctgattgagt tgatgaagaa atctgaagaa aacaagaaaa cgaaacaacg
    20401 ctatttcatc aagatggcgg ataaagtcgt caaatataat ttcttggggg ctgggctgac
    20461 ttacctattg acaggttctt tttctaaggc tatttctttc ctattggtcg atttctcctg
    20521 cgctttgaaa atctctactc ctgtagctta tttgacagtt atcaaggaag ggttgaaccg
    20581 tgaaatggtg attaaggatg gagatgttct ggagaaatat ctggaagttg atactttctt
    20641 gtttgataag acaggaacaa tcacaactag ttatcctata gttgaaaagg tgttaccttt
    20701 tggagactat agtgaggaag atattctcag aatcagtgcc tgtcttgagg aacacattta
    20761 tcatcctatt gctaatgcca tcgtcaagca agctgagata gagggaattg aacatgagga
    20821 aatgcatggg aaactccaat atatcgcaag caaggggatc aaatctcata tagatggcca
    20881 accagttctt attgggaatt atgtcttgat gcaggatgag cagattcata tcagttcaga
    20941 acaaaatgct ttaattgaag agtacaagag tcactacaat ctcttattct tggcttatca
    21001 aaatgaattg attggaatgt tctgcattca tactcctttg agaaaagaag caaaaacagc
    21061 cttggataaa cttaaggcac aggggaaaaa attgattctg gcaacaggag acaccttgat
    21121 taggacagag gaattagtca aagatttgcc ctttgatcag gtctatacag acttgaaacc
    21181 tgatgggaaa tttgagttag tagagaaact gcagaaagca ggtcacacta ttttgatggt
    21241 tggagatgga ttgaatgact cagcggcttt aactctatca gatatcggtg tggtgatgaa
    21301 tgagagtgca gatatttcta agcagatgag tgatatctta ttgttagata atcgtttgga
    21361 tttcttccaa gagttggatt cgttatcatc atctttgcaa acactcatca agaaaaatat
    21421 tcaggatacc gttgtcgtaa atagtagttt gattggcttt ggcttattta attggttcag
    21481 tccttcaaat ctctctatct tacataatct aacaaccttg cgcattgtcc tgcgtagcct
    21541 gtctattaaa aatagataga tgagagttat taacagcaaa aaggagttac ctttctcgag
    21601 gttaactcct ttgtttatag ataaatgttg aatagtttag taagtcaata caaagtattt
    21661 tttcttccca cgacggataa cagtcagttc attctctaac ttatcagcgt cactcaagac
    21721 atagtcaagc tcttggatgc ggtcgccgtt tacgtagatg gctccgtttt ggacgtcttc
    21781 acgggcttgg cgttttgagt taactatacc agatgagacg agcagttcca cgatattgtt
    21841 gttttcgtct gcctgtactt ggtagttggg cacaccacga agtccttgtt tgagctcttt
    21901 gacagaaagg tttttgatgt ttcctgcaaa gagttgctca gtgatgttaa gtgcttcttt
    21961 gtaggcttct tctccgtgaa caagtgtaac aacttcacga gccaagactt tttgagccaa
    22021 gcgttcgtgt ggcgctgctt caaattgttt acgaatatct tcaatctcat caagtgacaa
    22081 gaaagtaaag attttcaaga agcgaacagc gtcagcgtcc atcacgttca tccagaattg
    22141 gtacatttcg tatggagaag tcttttcggg attgagccag acggcatttc cttctgattt
    22201 accaaatttc ttaccagttg catctgtgat tagtggaaca gtgataacgt gaccagtctt
    22261 gtccgcctta cgacgaagca attcggtacc agctgtcata tttccccact ggtcagaacc
    22321 accgatttga agagtgacat tatggtcttg gttaaggacg aagaagtcat acccttgcat
    22381 gatttggtaa gcgaactcag tgtaagaaat tcctgtttcg atccgttttt taacagattc
    22441 cttactcatc atgtagttga ccgtgaagta ttttccaata tcacggagga agtcaatgaa
    22501 gctgatgctg ccaaaccagt cgtagttgtt gaccatgaca gccttgtttt cgccattttc
    22561 aaagtcaaga aaacgagaaa gttgtccttg gatagacttg acccagccat ctactgtgtc
    22621 ttttgtttgg agactacgtt cagcatcttt gaaggacgga tctccgatga gacctgtagc
    22681 accgccaacg agcgcataag gtttgtgacc tgctagttgc aagcgacgac ttgtcaagat
    22741 tgcgacaagg tggcctaggt gaaggctgtc agcagttgga tcgtagccag tataataaga
    22801 aacttgacct tcttctaggg ctttacgcaa agcttcttca tcagtcgttt gaaatatcaa
    22861 accacgctct tttagctcat caaaaatgtg catgtgtttt ttctcctttt taaaattatt
    22921 gtttctacct attgtatcac aaacttgggc aatagcctag cagaataggg aagaaagtgg
    22981 ctattttatt gaaaatttcc cttttgtggt ataatagata aagtgaggaa atccatgcaa
    23041 aatcaattaa atgaattaaa acgaaaaatg ctggaatttt tccagcaaaa acaaaaaaat
    23101 aaaaaatcag ctagacctgg caagaaaggt tcaagtacca aaaaatctaa aaccttagat
    23161 aagtcagtca ttttcccagc tattttactg agtataaaag ccttatttaa cttactcttt
    23221 gtactcggtt ttctaggagg aatgttggga gctgggattg ctttgggata cggagtggcc
    23281 ttatttgaca aggttcgggt gcctcagaca gaagaattgg tgaatcaggt caaggacatc
    23341 tcttctattt cagagattac ctattcggac gggacggtga ttgcttccat agagagtgat
    23401 ttgttgcgca cttctatctc atctgagcaa atttcggaaa atctgaagaa ggctatcatt
    23461 gcgacagaag atgaacactt taaagaacat aagggtgtag tacccaaggc ggtgattcgt
    23521 gcgaccttgg ggaaatttgt aggtttgggt tcctctagtg ggggttcaac cttgacccag
    23581 caactaatta aacagcaggt ggttggggat gcgccgacct tggctcgtaa ggcggcagag
    23641 attgtggatg ctcttgcctt ggaacgcgcc atgaataaag atgagatttt aacgacctat
    23701 ctcaatgtgg ctccctttgg ccgaaataat aagggacaga atattgcagg ggctcggcaa
    23761 gcagctgagg gaattttcgg tgtagatgcc agtcagttga ctgttcctca agcagcattt
    23821 ttagcaggac ttccacagag tcccattact tactctcctt atgaaaatac tggggagttg
    23881 aagagtgatg aagacctaga aattggctta agacgggcta aggcagttct ttacagtatg
    23941 tatcgtacag gtgcattaag caaagacgag tattctcagt acaaggatta tgaccttaaa
    24001 caggactttt taccatcggg cacggttaca ggaatttcac gagactattt atactttaca
    24061 actttggcag aagctcaaga acgtatgtat gactatctag ctcagagaga caatgtctcc
    24121 gctaaggagt tgaaaaatga ggcaactcag aagttttatc gagatttggc agccaaggaa
    24181 attgaaaatg gtggttataa gattactact accatagatc agaaaattca ttctgccatg
    24241 caaagtgcgg ttgctgatta tggctatctt ttagacgatg gaacaggtcg tgtagaagta
    24301 gggaatgtct tgatggataa ccaaacaggt gctattctag gctttgtagg tggtcgtaat
    24361 tatcaagaaa atcaaaataa tcatgccttt gataccaaac gttcgccagc ttctactacc
    24421 aagcccttgc tggcctacgg tattgctatt gaccagggct tgatgggaag tgaaacgatt
    24481 ctatctaact atccaacaaa ctttgctaat ggcaatccga ttatgtatgc taatagcaag
    24541 ggaacaggaa tgatgacctt gggagaagct ctgaactatt catggaatat ccctgcttac
    24601 tggacctatc gtatgctccg tgaaaagggt gttgatgtca agggttatat ggaaaagatg
    24661 ggttacgaga ttcctgagta cggtattgag agcttgccaa tgggtggtgg tattgaagtc
    24721 acagttgccc agcataccaa tggctatcag accttagcta ataatggagt ttatcatcag
    24781 aagcatgtga tttcaaagat tgaagcagca ggtggtagag tggtgtatga gtatcaggat
    24841 aaaccggttc aagtctattc aaaagctact gcgacgatta tgcagggatt gctacgagaa
    24901 gttctatcct ctcgtgtgac aacaaccttc aagtctaacc tgacttcttt aaatcctact
    24961 ctggctaatg cagattggat tgggaagact ggtacaacca accaagacga aaatatgtgg
    25021 ctcatgcttt cgacacctag attaacccta ggtggctgga ttgggcatga tgataatcat
    25081 tcattgtcac gtagagcagg ttattctaat aactctaatt acatggctca tctggtaaat
    25141 gcgattcagc aagcttcccc aagcatttgg gggaacgagc gctttgcttt agatcctagt
    25201 gtagtgaaat cggaagtctt gaaatcaaca ggtcaaaaac cagagaaggt ttctgttgaa
    25261 ggaaaagaag tagaggtcac aggttcgact gttaccagct attgggctaa taagtcagga
    25321 gcgccagcga caagttatcg ctttgctatt ggcggaagtg atgcggatta tcagaatgct
    25381 tggtctagta ttgtggggag tctaccaact ccatccagct ccagcagttc aagtagtagt
    25441 tctagcgata gcagtaactc aagtactaca cgaccttctt cttcaagggc gagacgataa
    25501 ttctctaaat gaagtggcca atcaatggat tgccactttt ttcctttcgt gttacaataa
    25561 gggtatgaat cagtatcaga aaaagattgt taacggaaaa atttattcgc tcctatccgg
    25621 cttaatatgg ggaatctgtg gaattttagg agagtacttc tttactcatt atcaggtgtc
    25681 ttcgggctgg attacctcta tgcgtttgac actggcaggg agtcttgtac tcatttggtc
    25741 tgcaatacaa ttaaaatcgc aagtgctaga tatttggcga gacaagaaaa attacctgcc
    25801 ctttttagcc tatgctattt tggggatttt ttcagttcag tattttttct atctctgtgt
    25861 agaatactca aatgctacga cagcaactat tttacagttt attagccctg tctttatcct
    25921 cttttacaat cgcttggttt atcaaaaacg agcgtcaaaa agcgctgttt tctatgtttt
    25981 ggttgccatg ctgggtgttt gcttgatggc gacaaaggga gacctctctc agttatccat
    26041 gacgccgcta gctcttataa caggtttgct gagtgccatg ggtgttatgt ttaatgttat
    26101 cttgccccaa ccttttgcta agcgttatgg ttttgttcct acggttgggt gggggatgat
    26161 tttggcaggt ttgtttagca atgtcctctc gccggtttat cagctttcct ttactcttga
    26221 tatttggagt atcttgattt gcctcattat cgctttcttt ggaacggctt ttgctttttt
    26281 catttccatg aaggctgtgt ccttggtttc tcctttggtg gttgccgtta tcagtgccag
    26341 tgaacctctc tcttctgctc tcttgagtgt tttgttctta ggattagtag tggattggtc
    26401 cctccttcta gctatagcct tgattatttt acccatgata tttttgtcta tagaagaagc
    26461 gaaagaaagt agataaaaag tcttttctta attctaaaag tgtgctatac tggaatagtc
    26521 aataaaacac ggggagatag ttgtgaagag tgtttttagg ttgtatcggt aggggcttgc
    26581 ttttaccgac agcacgtttt atctcacatc cctaaaaaat catacctaaa aacagacaaa
    26641 aaggtttcaa atttgaggcc ttttttggta gaataggtat cattataacg aaccaggagg
    26701 cacctatgac tgctacaaaa atgaacgctc aagaaattat ccaatttatc gccaatgctg
    26761 aaaagaaaac cagtgtcaaa gtaacctttg aggggcaact cgcaactgct gtgcctagct
    26821 ctgttgtcaa actaggaaat gtcctattcg gagactggaa ggatgtggct ccgcttcttg
    26881 aaggtttggt agaaaatcaa gattatgttg tcgagcaaga tgctcgtaat tctgcagttc
    26941 ctttgctaga taagcgtgct atcaacgctc gtatcgagcc aggtgcgatt atccgtgacc
    27001 aggtggaaat tggtgacaat gctgttatca tgatgggatc tgttatcaat atcggtgctg
    27061 aaatcggtgc tggaaccatg attgacatgg gtgccatcct tggtggccgt gccatcgttg
    27121 gaaaaaatag ccacgttggt gcaggtgcag ttttggcagg tgtgattgag ccagctagtg
    27181 ctgaaccagt ccgtgtcgga gacaatgttc ttatcggtgc taatgcagtg gttatcgaag
    27241 gagtccaaat cggtagtggt tcagttgtcg cagcaggagc tattgttacc caagatgtcc
    27301 cagaaaacgt ggtagtagca ggtgttccag ctcgtattat caaagaaatt gatgcccaaa
    27361 ctcaacaaaa aacagcgcta gaggatgcgc ttcgtacctt gtaattgtaa aagtaaaaaa
    27421 gaggcggaac cctttttcca gcctctttct gctatatcgg aggacagata gatgttagat
    27481 ttgattcaga ctagacgaga tttacaccag attccagaga ttggcttgga ggagttcaag
    27541 actcaggctt atttgctgga tgtgattgag aaattgacta cgggcaagga ttttgttcaa
    27601 attcgtactt ggcggacagg gattttggtc tacctgcagg gaagtcagcc ggagcgaacc
    27661 attggttggc ggacagatat tgatggcctg cctatcgtcg aacaaacagg tctacctttt
    27721 gcttctcaac accaaggtcg catgcatgcc tgtggccatg attttcatat gaccattgcc
    27781 ttaggctgtc ttgagcgcgc ccttgaggag caacccaaga ataatctgct ctttctattt
    27841 cagcctgctg aagaaaatga agctggtggt atgctcatgt atgaggacgg tgcttttgga
    27901 gattggttgc cagatcaatt ctatggtctc catgttcgtc cagatttgaa agtcggccag
    27961 attgcgacta atactcatac actctttgca ggaacttgcg aggtgaagat ccgtttcaaa
    28021 ggcaaaggtg gccacgcagc ttttcctcat gaagccaatg acgccttggt ggcggctagt
    28081 tactttgtga cccaagtgca gtcagttgtt agccgcaatg tcaacccaat cgagggagca
    28141 gtggtgacct tcggcgtttt tcaagctgga accaccaaca atgtcatcac agacacagct
    28201 tttttacatg gaactattcg tgccttgaca caggacatga gtctcttggt gcaaaagagg
    28261 gtaaaaacag ttgcagaagg ggttgcagct gcctttgata tggaagtcga agtggaactc
    28321 aaacaaggtg gatacctacc tgttgagaac aatccagcct tggcgcgtga actgatggac
    28381 ttctttgacg aaaaagacgg aatcgagttg attgatatcg aacctgctat gacaggcgag
    28441 gactttggtt atctcctttc aaaggttgat ggcgtgatgt tttggctagg tatcgatagt
    28501 ccctacgctc ttcatcaccc tcaaatgagt cctaaggaag aagtcttagc tattggggtg
    28561 gctgcggtgt ctagtttcct gaaaaagaag gcagcagaat agagggcttg tctatgaaat
    28621 cggaattacg caagcaagtc ttgcatgaaa tgaaggctat atctcaagag caaaaacagg
    28681 ctatagacca agctttaacc gagcggcttt tacaacaccc cttttatcaa gaagccaagg
    28741 tcatcgcaac ctacctctct ttttctcatg agtttcaaac gcaggaactg attgagcagg
    28801 cgctgaagga cggcaagaag gttttaatac ccaaaactta tcccaagggg cgcatggact
    28861 ttgtggtcta tgatccgcaa cagttggtaa aaactttctt tgccttactg gagctacagg
    28921 gagatttgga agtggtggat gcatctcaga ttgatttgat tcatgttcct ggtctggctt
    28981 ttacgacgaa aggatatcgg ataggatacg gtggaggcta ttatgaccgc tatctggaac
    29041 atttttctgg tcgtactttg agtacgatat atccttgtca aattcaggac tttatacctg
    29101 aaaaccatga tattcctgtt caggaggtat taattgatga aggaaatctt tgatagacgt
    29161 taccctgtga cgagtttctt cctcttagtg acggccttgg tatttttact aatgttggtc
    29221 actgcaggcg gaaactttga cagggcagat acattatttc gatttggagc catgtatggg
    29281 ccagctattc gcctctttcc cgagcaggtt tggcgtctct tgtctgccat ttttgttcat
    29341 attgggtggg aacatttcat tgttaatatg ctttcacttt attatcttgg aaggcaggta
    29401 gaggagattt tcggttctaa gcagtttttc tttctctatc ttttatcagg aatgatgggc
    29461 aatctctttg tttttgtatt tagtcctaaa tccttagcag caggcgcctc tacctctctt
    29521 tatgggctat ttgccgcgat tattgttctt cgctatgcaa ctcgcaatcc ttatatccaa
    29581 cagctagggc aatcctatct gacacttttt gtggttaaca ttattggaag tgttctgatt
    29641 ccaggaatca gcctagcagg ccatatcggt ggtgcagttg gtggcgcatt tctagcagtt
    29701 atctttccag ttagaggaga aaaacggatg tataacacca gccagagatt aggagcggta
    29761 gtcttgttcg taggactcgc cattttgctt ttctacaagg gaatgggatt gtgacgacta
    29821 atactcaatg aaaatcaaag agcaaactag gaagctagcc gcaggttgct caaagcactg
    29881 ctttgaggtt gtagatagaa ctgacgaagt cagctcaaaa catggttttg aggttgtaga
    29941 tgaaactgac gaagtcagta accataccta cggtaaggcg atgctgacgt ggtttgaatt
    30001 tgattttcat tgagtataat atgtaaactt gaataagaaa aaagctactt aaaaaatgag
    30061 tagcttttaa tgtgttttgg aaccattcga aacaacacag ctctaaaata gttattagcg
    30121 ttgactaggt tctgcaatgc cgccaccaaa gttcccaagg taatttccat cgtcatcata
    30181 cacatctcca ttattatgat ctttgtagta gtatctttga tagccgtcag caccagtgcc
    30241 tttatatctg tagacattat ttgctccgct gtttgaattg ctagatgaat attgttctga
    30301 tgatccacca tttgttgaac tatctcctga accagaataa tcttgaccgc cataattatt
    30361 tgaatatcca ttagtttgtt cagatgaact ataatctaca tttgaattgg aagatccatt
    30421 ataagactca ttagaagcac taccagaaga attactttga ctagaagatt cctttgcttt
    30481 ttcagcagct ttttcagcag ctttttcttc ttctttcttc aaaagttcga tttgtttttg
    30541 atatttatca acaagatcat ttagagattt aagagtttct tccaaattct cttgtttaaa
    30601 agtttcatca acttttttga taaaatcaat ttgttttgta atttctttta catttgtttc
    30661 aagagttgct ttatctttaa atttgtcatc tgaatcttta acatcataag ccaaaccttt
    30721 ttgcaaatga aaatctacaa ttgctttaga tgattctttg actttgttat taagatagtc
    30781 tttacctgca tcggttttaa gtgaagtttc agatagttct tttaatttag aaatagattc
    30841 atctgaagat gcatcaaatg attcaaatag tttatcaact tgttctttgt ctgatttgct
    30901 atctttttct aattgtacaa tttttgcttg ttgttctgct tgttgttggt tatatgaata
    30961 ccatccaaac cctaatacca atacaacacc aactgcaact gatgaaatga ttactttttt
    31021 attgttttcg ataaattcta caaatttgtt ctccataatc tttttctttg atagtgtccg
//
LOCUS       DAVKNL010000024        29896 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVKNL010000024 DAVKNL010000000
VERSION     DAVKNL010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29896
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.024"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            <1..950
                     /gene="cps4G"
                     /locus_tag="VW411_001049"
     CDS             <1..950
                     /gene="cps4G"
                     /locus_tag="VW411_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4G"
                     /protein_id="HEU9793795.1"
                     /translation="ITKQNYYRISFSGKGKLSKILGYVKFRKEIKKKLKENDYDMILP
                     LHSIVSFILVDFLLFSFKNRYIYDIRDYSYEKFLVYRLVQKQLVKNSLMNIVSSDGYK
                     FFLPMGEYFTTHNLPNMIELNEVKQLKNNSTFPIQLSYIGLIRFQEQNKKIIDFFAND
                     SRFQLNFIGTNAGELREFCQEKNISNVNLVDTFQPKDTMSFYKNTDVVLNLYGNHTPL
                     LDYALSNKLYFAALLYKPILVCEDTYMEKVSIENGFGFVLPMKDESEKDCLALYIQNL
                     DRKQLIKNCDNFMDRISLEKQKTEIELEKRILSLRKKND"
     gene            943..2061
                     /gene="cps4H"
                     /locus_tag="VW411_001050"
     CDS             943..2061
                     /gene="cps4H"
                     /locus_tag="VW411_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4H"
                     /protein_id="HEU9793796.1"
                     /translation="MIKVLHLFTTLDSGGVESFLFNYYSHIDRKKIQFDFIVPGKEQG
                     FLEDKMKELGAKVYHVPLLRKKPLHQFLSLARIIKKGDYDIVHCHGYKSAIGLILSKI
                     IGCKIRIIHSHMAYVTENSFQKVLRKLVTILVKILATHWFACGEDSAKWLYGEKAYKD
                     GKIEIIFNAIDLKKYQFLSDVREKCRRELDVSNKFVLGNIARLSDQKNQSYLFNVLKE
                     LILIKPNVILLLVGNGEDEQKLKQKALELNLTPYVLFLGRRTDISDLLSAMDVFLLPS
                     KYEGLPVSLVEAQASGLQILSSDTVTQEVDVTKNISYLPINEESVLLWKDKVLSLTSE
                     ECNRFEINNSMTDGLYDICYQASKLLNRYQEMCVIKEI"
     gene            2062..3462
                     /gene="wzy"
                     /locus_tag="VW411_001051"
     CDS             2062..3462
                     /gene="wzy"
                     /locus_tag="VW411_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HEU9793797.1"
                     /translation="MQTKYICRVTLVTLSFIFAFCYLFWTLDNWNNGFLISNYVPSIF
                     IWVCFLIIFQITGFILQKVSIYDFSVWYLILSYFFMFGLIFNEYMGFQTTLLWSPSNF
                     YNNEELFHSYIFIIWILFCYSVGYLFFYSDGKVHYHSEVQNYQENEEKILYNAGRILT
                     GVGFISRVITDSKTVLAVRAANSYSAYSEAASSGIIDDLGVLMLPGVFSLFYSDKLSR
                     VIKRTIFWVMLFYLILIMILTGSRKIQVFSILALVLVYTQSLGITFSKKRVLVFLIVT
                     VFLLNVLVVIRGHRFDLNTIGIYLFDSFSSLDFVKNILGEVFSESGLTSLTVASAVTV
                     VPSSIPYEYGMTFLRTILSIFPIGWLVGDFFDKASATVVINKFLGLPVGSSFVEELFW
                     NFGYYGGVFWSFVLGIFSGWRLNFRAFQTSKISKVIYFSVISQLLLLVRSSSIDVYRP
                     IMYSLIMIFIFRRLKK"
     gene            3477..4544
                     /locus_tag="VW411_001052"
     CDS             3477..4544
                     /locus_tag="VW411_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HEU9793798.1"
                     /translation="MLHGATDYGSSNYGDYLYGEIVYDLLESKGYEVSFYNPSDFFQM
                     YLKEYRQKQSFTKKQADAILYIPGGYFGEGHNARFRDNLIQFKRFLPLGIWASYFKKP
                     IGVLGIGAGPNNDSLMNYGIKRIINHAQFITVRDRESFDSLKHLSPSAPVHETFDLII
                     SSKLREEKTEQLCQLKREAKDKKIILVHYNHSKKALEKFAESISLFLENNPNYYVVVT
                     SDSILPYEDAYYQEFRKLVRTEDCFQFKYHSPAEMTSLLKMVDVVLTCKLHVGVVATC
                     FNKSVIAIACHPEKTARYYGAIGELQRCESLFDSSVNSIVKKLETFHLKPITIPSELV
                     LKARSSLDYLDLFLEGLVRES"
     gene            4534..5775
                     /locus_tag="VW411_001053"
     CDS             4534..5775
                     /locus_tag="VW411_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEU9793799.1"
                     /translation="MKVDRISFIKNTSSLYILNIVKLLFPLLTLPYLTRVLSLDAYGM
                     VIYVKALIAYVQLVIDFGFMISATKNIVNACTTPSKIGRIVGDTLVEKIFLSIISILI
                     YTILMWQIPIMRENILFSVFYLLATVTNIFIFDFLFRGIEKMHAVAIPYIISKTIITI
                     LTFIVVKDDSSILWIPILEGIGNLVAAVVSYRFLHYYGIKLSFSYLSVWVKDLKESSI
                     YFLSNFATTIFGVFTTVISGFYLQSQEIAFWGIAMQLLSAAKSLYNPIANSLYPHMIR
                     TKDIQSVKSINRIMFIPIIFGVLIVLFFSNQILSIIGGEKYTVSADFLKYLLPAFVAS
                     FYSMIYGWPVLGAIDKVKETTMTTILASIVQTLGLGIFILSDNFSLVTLAICSSMSEV
                     VLWISRYLIYFKNRSLFVRSK"
     gene            5780..6877
                     /gene="cps4I"
                     /locus_tag="VW411_001054"
     CDS             5780..6877
                     /gene="cps4I"
                     /locus_tag="VW411_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000758382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4I"
                     /protein_id="HEU9793800.1"
                     /translation="MKKVVVVFGTRPEAIKMCPLVKELRTRKNIETLVCVTGQHRQML
                     DQVLDTFGIIPDFDLSIMKDKQTLFDVTIGILEGMKAILESEKPDLVLVHGDTSTTFA
                     SSLAAFYLQIPIGHVEAGLRTYDIYSPYPEEFNRQAVGVLAQYHFTPTQLSKDNLLRE
                     GKTPESIFVTGNTAIDALQTTIQEDYTHPELEWIGESRFILITAHRRENLGEPMRHMF
                     RAIRRIIEEYSDVKAIYPIHMNPRVRQIAEEELSGCERIKMIEPLEVLDFHNFLSRSY
                     LILTDSGGIQEEAPSLGKPVLVMRDTTERPEGIEAGTLKLVGADENNIYRHFKELLEN
                     DSVYQAMSQASNPYGDGTACKKIADILEGEV"
     gene            6881..7936
                     /gene="cps4J"
                     /locus_tag="VW411_001055"
     CDS             6881..7936
                     /gene="cps4J"
                     /locus_tag="VW411_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000077424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4J"
                     /protein_id="HEU9793801.1"
                     /translation="MSQFTGKTLLITGGTGSFGNAVLKRFLETDVSEIRIFSRDEKKQ
                     DDMRHEFQVKVPEVAGKIRFYLGDVRDLASVKNAMHGVDYVFHAAALKQVPSCEFFPV
                     EAVKTNILGTENVLTAAIEAGVKQVICLSTDKAAYPVNAMGTSKAMMEKIAVAKSRTV
                     NPEHTKICVTRYGNVLCSRGSVVPLWIEQIKQGNALTITEPSMTRFVMTLEEAVDLVL
                     FAFEEGKSGDILVQKAPACTIEVLAKAVSEIFASEQDIKIIGIRHGEKRYETLLTNEE
                     CANAIDLGDFYRVPSDNRNLNYDKYFKDGSTNRNLLTEFNSNNTDLMDVEQVKRKLLE
                     LDEIQTAIRDMVADEEM"
     gene            8036..9265
                     /gene="cps4K"
                     /locus_tag="VW411_001056"
     CDS             8036..9265
                     /gene="cps4K"
                     /locus_tag="VW411_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4K"
                     /protein_id="HEU9793802.1"
                     /translation="MIKNILITGAKGFVGKNLICTLEALKDGRDRTRPNLEIGEIFQY
                     DRDTDPILLDEYCKKADFVFHLAGVNRPQNPDEFMEGNYGFSSRLLEILEKYENTCPV
                     LLSSSTQASLEGRFSNSIYGQSKLAGEELFFEYGKKTGAPVLVYRFPNLYGKWCRPNY
                     NSAVATFCYNLAHDLPIQVNDPSVELELLYIDDLIQECLTALEGNPHRCNLDGLQILP
                     SPSGNYCYVPTTHRATLGEIVSLLETFKKQPDSLVMPEIPQGSFKKKLYSTYLSYLPV
                     DKFKFPLKMNIDERGSFTELLKTENTGQFSVNISKPGITKGQHWHHSKWEFFMVVSGR
                     ALIQERRIGLDENGQEYPILNFEVSGDKIEAIHMIPGYAHNIINLSDTENLITVMWAN
                     ESFDPRHPDTFFEQVEK"
     gene            9266..10450
                     /gene="cps4L"
                     /locus_tag="VW411_001057"
     CDS             9266..10450
                     /gene="cps4L"
                     /locus_tag="VW411_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4L"
                     /protein_id="HEU9793803.1"
                     /translation="MKIKTDYSDIHFKDNGKLKLLIIVGTRPEIIRLSSVITKCRKYF
                     DVILAHTGQNYDYNLNGIFFDNLGLDTPDVYMDAVGDDLGATVGNIINTSYKLMNQIK
                     PDALLILGDTNSCLSAIAAKRLHIPIFHMEAGNRCKDECLPEETNRRIVDVISDVNLA
                     YSEHARKYLHECGLPKERTYVTGSPMAEVLHKNLSAIESSDIHERLGLKKGGYILLSA
                     HREENIDTDKNFISLFTAINQLAEKYNMPILYSCHPRSKKRLQESGFKLDKRVIQHEP
                     LGFHDYNCLQMNAFVVVSDSGTLPEESSFFTSQGYPFPAVCIRTSTERPESLDKAGFI
                     LAGIDENSLLQAVETAVSLAQDEDFGLPVPDYVEENVSTKVVKIIQSYTGIVDKIVWR
                     KS"
     gene            complement(11041..11617)
                     /locus_tag="VW411_001058"
                     /pseudo
     CDS             complement(11041..11617)
                     /locus_tag="VW411_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(11693..11920)
                     /locus_tag="VW411_001059"
     CDS             complement(11693..11920)
                     /locus_tag="VW411_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001815354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9793804.1"
                     /translation="MRERLLTQPLLHADETSYRVLESDSHLTYYWTFLSGKAEKQGIT
                     LYHHDQCRSGSVVQEFLGDYSGYVHCDMLRQ"
     gene            11948..12256
                     /locus_tag="VW411_001060"
                     /pseudo
     CDS             11948..12256
                     /locus_tag="VW411_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12411..12734)
                     /gene="tnpB"
                     /locus_tag="VW411_001061"
     CDS             complement(12411..12734)
                     /gene="tnpB"
                     /locus_tag="VW411_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU9793805.1"
                     /translation="MTIHLSSLGQVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQI
                     FLFCGGRKDRFKVLYWDGQGFWLLYKRFENGRLTWPSTEKDVKALAPEQVDWLMKGFS
                     ITPKI"
     gene            complement(12715..12900)
                     /locus_tag="VW411_001062"
     CDS             complement(12715..12900)
                     /locus_tag="VW411_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793806.1"
                     /translation="MPQPIVPVEIPQSRRFDSKKRNDILLKIRIGKLEVSFFQSLNLE
                     MIEQLLDKVLLYDNSSI"
     gene            13105..15087
                     /locus_tag="VW411_001063"
     CDS             13105..15087
                     /locus_tag="VW411_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9793807.1"
                     /translation="MKSSKLFALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHVDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNLFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVQFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDETTDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
     gene            15389..20692
                     /locus_tag="VW411_001064"
     CDS             15389..20692
                     /locus_tag="VW411_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU9793808.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVANKEQQGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKDDSEKGKSRFGVFLKFKDTKNNVFVGYDKDGWF
                     WEYKSPTTSTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYDDERTVVSVKTDNQEGVKTEDTPAEKETGPEVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDESKLLSSISF
                     LGNALVSVSSNQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKNVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRDNATVDDTSYFQNMYAFFTTGADVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HNPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVADD
                     FASLTAPAQAQEGLANAFDGNVSSLWHTSWNGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPNNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALVKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLRSLASEQGKTPDYKQEIA
                     RPETPEQILPATGESQSDTALILASVSLALSALFVVKTKKD"
     gene            complement(20859..23018)
                     /locus_tag="VW411_001065"
     CDS             complement(20859..23018)
                     /locus_tag="VW411_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEU9793809.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLTYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWNSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(23015..23611)
                     /gene="recU"
                     /locus_tag="VW411_001066"
     CDS             complement(23015..23611)
                     /gene="recU"
                     /locus_tag="VW411_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEU9793810.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            23677..24204
                     /locus_tag="VW411_001067"
     CDS             23677..24204
                     /locus_tag="VW411_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEU9793811.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDELNELAENFSEN"
     gene            24274..24603
                     /gene="gpsB"
                     /locus_tag="VW411_001068"
     CDS             24274..24603
                     /gene="gpsB"
                     /locus_tag="VW411_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEU9793812.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            24624..25004
                     /gene="rnpB"
                     /locus_tag="VW411_001069"
     ncRNA           24624..25004
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VW411_001069"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            25089..26246
                     /locus_tag="VW411_001070"
     CDS             25089..26246
                     /locus_tag="VW411_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEU9793813.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKANA
                     QVAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            26259..27653
                     /gene="mapZ"
                     /locus_tag="VW411_001071"
     CDS             26259..27653
                     /gene="mapZ"
                     /locus_tag="VW411_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEU9793814.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVESGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            27729..29153
                     /gene="gndA"
                     /locus_tag="VW411_001072"
     CDS             27729..29153
                     /gene="gndA"
                     /locus_tag="VW411_001072"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEU9793815.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            29165..29854
                     /locus_tag="VW411_001073"
     CDS             29165..29854
                     /locus_tag="VW411_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9793816.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLMLLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVLIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
BASE COUNT         9383 a         5015 c         6382 g         9116 t
ORIGIN      
        1 ttattacaaa acaaaactac tatcgtattt ctttttctgg taaaggaaaa ttaagtaaga
       61 tattaggtta tgtaaaattc agaaaagaaa ttaaaaagaa gctaaaagaa aatgattatg
      121 atatgatatt gccgttacat agtattgtgt ctttcatttt agtagatttt cttctctttt
      181 catttaaaaa tagatatatt tatgatattc gtgattacag ttatgaaaaa tttttggttt
      241 atcgtttggt tcagaaacaa ttggtgaaaa attctttaat gaatatcgtt tcttcagacg
      301 gctataaatt ttttttacca atgggagagt attttactac ccataaccta cccaatatga
      361 tagaattaaa cgaggtaaag cagttaaaaa ataatagtac gtttccaatt caactttcct
      421 acattggttt aattcgtttt caagaacaaa ataaaaaaat aatcgatttt tttgcaaatg
      481 acagtcgatt tcagttgaat tttataggta ctaatgcagg agaattaagg gaattttgtc
      541 aagaaaaaaa tatcagcaat gttaacttgg tggacacatt ccagcctaaa gataccatgt
      601 ctttttataa gaatacagat gttgttttga atttatacgg gaatcacact cccttactag
      661 actatgcttt gtctaataaa ctctactttg ctgcattgtt gtataaacct attttagttt
      721 gtgaagatac ttatatggaa aaagtgtcaa tagagaatgg ttttggtttc gttttaccta
      781 tgaaggatga atcggaaaaa gattgtttgg ctttgtatat tcaaaacctt gacaggaaac
      841 agctaattaa gaattgtgat aacttcatgg acagaattag tttagaaaaa caaaaaacag
      901 aaatagaatt agagaagcga atcttaagct taaggaagaa gaatgattaa agtattacat
      961 ctatttacaa cactagatag tggtggagta gaaagttttc tattcaacta ttattctcac
     1021 attgatagaa aaaaaattca atttgatttt attgtgcctg gaaaagaaca aggattttta
     1081 gaggataaaa tgaaagaatt gggtgcaaag gtttatcatg tgcctctatt aaggaaaaag
     1141 cctctacatc agtttctctc tcttgctaga ataataaaga aaggagatta tgatatagtt
     1201 cattgccatg gctataaatc tgcaattggt ctgatcttat ctaaaataat tggttgtaaa
     1261 attagaatta ttcatagtca tatggcttat gtaacagaaa acagttttca aaaagtattg
     1321 cgtaaattag taacaatttt ggtaaaaatc ttagcaactc attggtttgc atgtggggaa
     1381 gattcggcta agtggttata tggagagaaa gcgtataaag acggaaaaat tgaaattatt
     1441 tttaatgcaa ttgatttgaa aaagtatcaa tttttgtcag atgttagaga aaaatgtcgt
     1501 agagaattag atgtgtcaaa taagttcgta ttaggaaata tagctcgcct atcagatcaa
     1561 aaaaaccaaa gttatttatt taacgtttta aaagaactca ttttaatcaa accaaatgtt
     1621 attttactcc tagttggtaa tggtgaggat gagcagaaat taaaacagaa agctttagaa
     1681 ctaaatctga ccccatatgt gctattttta gggagaagga ctgatatttc tgatttatta
     1741 tctgcgatgg atgttttttt gcttccgtct aaatatgagg ggttgcctgt ttctctagta
     1801 gaggctcagg catcgggatt acaaatttta tcgtcagata cagtgacgca agaagtagat
     1861 gtgaccaaaa acattagtta cttacctatc aacgaagagt ctgtgttgct atggaaagat
     1921 aaagtactgt ctttaacatc tgaggaatgc aatcgttttg aaataaataa cagtatgaca
     1981 gatggactct atgatatttg ttatcaagct agtaaattat tgaatcgtta tcaagaaatg
     2041 tgtgtaataa aggagatata gatgcagaca aagtatattt gtagagtgac actagtgaca
     2101 ttaagtttta tatttgcttt ctgttacttg ttctggactc tcgataattg gaataatggt
     2161 tttcttatat ctaattatgt tccgtctata tttatatggg tttgctttct aattattttt
     2221 caaattactg gttttatttt acaaaaagtt agtatatatg atttttctgt atggtatctg
     2281 attttatctt atttttttat gtttggatta attttcaatg agtatatggg gtttcaaaca
     2341 actctgctgt ggagccctag taacttctat aataatgaag aattatttca ttcatatatt
     2401 tttataattt ggattttgtt ttgttattct gtaggctatt tattttttta tagtgatgga
     2461 aaggtacatt atcattcaga agtacaaaat tatcaggaaa atgaagagaa aattttgtac
     2521 aatgcgggta ggattttaac aggagtgggc tttatttcta gggtaataac tgattctaaa
     2581 acagtactag cagttagagc ggcgaatagc tattcagcat attcagaggc agctagttca
     2641 ggaataatag atgatttagg agtacttatg cttcctggtg tgttctcctt gttttattca
     2701 gataagctgt cccgagttat aaagcgtact attttttggg taatgttgtt ttacctaatt
     2761 ttgataatga tcctaacagg aagtcgtaaa atccaggtgt tttcaatctt ggcgttggtt
     2821 ttagtataca cacaatcttt agggattact ttttcgaaga aaagagtact tgtattttta
     2881 atagtaactg tatttctatt aaatgtatta gttgttatta gggggcaccg tttcgacttg
     2941 aatactatag gtatatactt atttgattcc ttttcttcat tagattttgt aaagaatata
     3001 ctaggggaag tattttcaga gtcgggactg accagcctaa cagtagcttc tgctgtaact
     3061 gttgtgccta gtagcattcc atatgaatat gggatgacat ttctacgcac tatcctatca
     3121 atttttccta ttggatggtt agttggtgat ttttttgata aagcatcagc gacggttgtt
     3181 attaataaat ttttggggtt acctgtaggc tcttcttttg tggaagaatt attttggaac
     3241 tttggttact atggtggtgt tttttggagt tttgttttgg gaatatttag tggatggcga
     3301 ttaaatttca gagcttttca aacaagtaaa atctcaaaag ttatttattt ttcggtaatt
     3361 tctcaactat tgcttttagt gagaagtagt tcaatagatg tttatcgtcc tattatgtat
     3421 agtttgatta tgatatttat ttttaggagg ttgaaaaaat agtggtgaaa aaaattatgt
     3481 tacatggtgc aacggattat ggttcaagta attacggtga ttatctttac ggtgagattg
     3541 tctatgattt attagaatct aaaggatatg aagtttcttt ttataatcca tcagatttct
     3601 ttcaaatgta cttgaaagaa tatcgccaaa aacaaagttt tactaaaaaa caagcagatg
     3661 ctattttgta tatcccggga ggatattttg gggaagggca taatgcacgt tttcgtgata
     3721 atttaatcca atttaaacga tttttacctt tgggaatttg ggcttcatat tttaaaaagc
     3781 ctataggagt attaggtatt ggtgcagggc ctaataacga ttctttgatg aattatggta
     3841 taaagagaat tattaatcat gctcaattta ttacagttcg tgatcgagaa tcatttgatt
     3901 ctttgaagca tttgtcgcct tcggctccag tgcatgaaac ctttgattta attatttcaa
     3961 gtaaattaag agaagaaaaa acagagcagc tgtgtcaatt gaaaagagaa gctaaagata
     4021 agaagataat tcttgttcac tataatcatt ccaaaaaagc tcttgaaaaa tttgcggaat
     4081 caattagtct ttttctagaa aacaatccta actattatgt tgtagtaact tcggattcca
     4141 ttctacctta tgaagatgct tattatcagg aatttagaaa attagttaga actgaggatt
     4201 gttttcaatt caagtatcat tctcctgcag aaatgacatc attgttgaaa atggtagatg
     4261 tggttttaac ttgtaaatta catgttgggg tagtggcaac atgttttaat aaatctgtga
     4321 ttgccattgc atgccatcca gaaaaaacag cacgttatta tggagcaatt ggagagttac
     4381 aacgttgtga atctttattt gattcaagtg ttaatagtat tgtaaagaaa cttgaaactt
     4441 ttcatctaaa accgataaca ataccttcag aattggtgtt gaaagcgaga agtagtttgg
     4501 actatttaga tctttttttg gaaggactag ttcgtgaaag ttgatagaat ttcatttata
     4561 aaaaatacaa gttctctcta tattctgaat attgttaaat tactatttcc tttattaaca
     4621 ctcccgtatt tgacaagggt gctttcgcta gacgcgtatg gaatggttat ttatgttaaa
     4681 gcgttaatag cttatgttca actggtgatt gattttggtt tcatgatatc agctacaaaa
     4741 aatattgtaa atgcttgtac tactccctca aagattggaa ggatagttgg agatactcta
     4801 gttgaaaaaa tatttttatc tatcatttcg attctaattt acaccatatt gatgtggcaa
     4861 atcccaataa tgagagagaa tattcttttt tcagtttttt atttgttagc tacagtgacc
     4921 aatattttta tctttgactt tttatttcgt ggaattgaaa agatgcatgc agttgcaatt
     4981 ccttatatta tttctaaaac tatcattaca attttgacat ttattgtagt aaaagatgat
     5041 tcttctattt tatggattcc tatattggaa ggaattggga atttagttgc tgcagtagtt
     5101 tcttatagat tccttcatta ttatggaatt aaattatcat tttcttatct gtctgtttgg
     5161 gttaaagatt taaaggaatc ctctatttat tttttatcca attttgcaac tactattttt
     5221 ggcgtcttta cgacagtcat ttcgggtttt tatttacaaa gtcaagagat agccttttgg
     5281 gggatagcaa tgcaactgct ttcagcagca aaatcattgt ataatcctat agcgaatagt
     5341 ttatatccgc atatgatacg tactaaagat atacaatcgg ttaagagtat taatcggatt
     5401 atgtttattc ctattatctt tggagttttg atagttttat tcttttcaaa tcaaattctt
     5461 tctataattg gtggtgaaaa atataccgtt tcagcagatt ttcttaagta cttattaccc
     5521 gcttttgttg ctagttttta ttctatgatt tacggatggc ctgtcttagg agctattgat
     5581 aaagtgaaag aaactacaat gacaactata ttagcttcga ttgtccaaac tttgggatta
     5641 ggaatattta tcttgtctga taattttagt ttagtaacat tagctatttg ttcaagtatg
     5701 tctgaggtgg tgttatggat tagccgttat ctaatttatt ttaagaaccg ttcattattt
     5761 gttaggagta agtaaatata tgaaaaaagt agttgtagta tttggaactc gtccagaagc
     5821 catcaagatg tgtcctttgg tcaaagagtt aagaacacgt aagaatattg aaacattagt
     5881 ttgcgttacg ggccaacacc gtcaaatgtt ggatcaggtc ttggacacat ttgggattat
     5941 tcccgatttt gatttgtcta ttatgaaaga taaacaaact ttatttgatg tgaccattgg
     6001 cattttggaa ggaatgaagg ctattttaga gtcagaaaaa cctgatttgg tgttagttca
     6061 tggggataca agtacgacct tcgctagttc actggcggcc ttttatttac agattccaat
     6121 tggtcatgtt gaagctggtt tgcgcactta cgatatctat tcaccatatc ctgaagagtt
     6181 taatcgtcaa gcagttgggg ttttggctca gtatcatttt acaccaacac aactttcaaa
     6241 agataatctt ttaagagaag gaaaaactcc agagagcata tttgtgacag gtaatacggc
     6301 gattgatgct ttacaaacta caattcaaga ggactatacg catcctgagt tagaatggat
     6361 aggagaaagc aggtttattc taattacagc tcatcgtcgt gaaaatcttg gtgaaccgat
     6421 gagacatatg tttagagcaa ttagaaggat tattgaagaa tattcagatg tcaaggctat
     6481 ttatcctatc catatgaatc ctcgtgttcg ccaaattgct gaggaagaac taagtggctg
     6541 tgaacgtatc aagatgatcg aacctttaga agtattagat tttcacaatt ttttatcaag
     6601 aagttattta attttaacag atagtggtgg tattcaggaa gaggctccaa gtttaggaaa
     6661 acctgttttg gttatgagag atacaactga acgtccagag ggaattgaag cagggacttt
     6721 gaaacttgta ggtgctgatg aaaataatat ttataggcat tttaaagaat tattagaaaa
     6781 tgattcagtt tatcaagcaa tgagtcaagc ttcaaatcca tatggagatg gaacggcttg
     6841 taagaaaatt gcagacatac tagaaggaga agtgtaatat atgtcacaat ttacaggaaa
     6901 aactctccta attacaggag ggacaggttc atttgggaat gcggttctca agcgtttttt
     6961 agaaacagat gtctcagaga ttcgtatatt ttcgcgagat gaaaagaaac aagatgatat
     7021 gcgtcatgag tttcaggtaa aagtaccaga agtggctggg aaaatccgtt tttaccttgg
     7081 agatgtgcgt gacttagctt cggtaaaaaa tgctatgcat ggtgtggatt atgtttttca
     7141 cgctgctgct cttaaacaag taccttcttg tgaatttttc cctgtagaag cggtaaaaac
     7201 taacattttg ggaacggaga atgtcctcac tgcggctatc gaagctggtg taaagcaagt
     7261 tatctgtctt tctacagata aggcggctta tcctgtcaat gctatgggaa catccaaggc
     7321 tatgatggaa aagattgctg ttgctaagtc caggacggta aatcctgagc atacaaagat
     7381 atgtgtgact cgctatggga atgttctgtg tagccgtgga tctgtcgttc ctctttggat
     7441 tgagcaaatt aagcaaggaa atgctcttac aattactgaa cctagcatga cacgttttgt
     7501 gatgacattg gaagaagcag tagacttggt tttatttgca tttgaagaag ggaaatctgg
     7561 agatatacta gttcagaaag caccagcatg tacgattgaa gtgttggcta aggctgtatc
     7621 agaaattttt gcctcagaac aagatattaa gattataggt attagacacg gagaaaaaag
     7681 gtatgaaaca ctattaacta acgaagagtg cgcaaatgca attgatttgg gtgattttta
     7741 tagagttcct agtgataatc gtaatcttaa ctatgataag tatttcaaag atggtagtac
     7801 gaatagaaat ttgttgacag aattcaatag taataatacc gatttgatgg atgtagagca
     7861 agttaaaaga aagttgctag aattagatga aatacagaca gcaattcgtg atatggtagc
     7921 agacgaagag atgtaaaatc ttttaagata cattaaaatt atcaaggata tataatgtat
     7981 catgtattgt cgagagagtt aactattaaa acagcatata aaataaggag ggggcatgat
     8041 taaaaatatt ttaatcacag gagcgaaagg atttgtagga aaaaatctta tctgtactct
     8101 ggaagctttg aaagatggac gagataggac tcgtcctaat ttagagattg gagagatttt
     8161 tcagtatgat cgtgatacag atccgatttt attagatgaa tattgtaaga aggccgattt
     8221 cgtattccat ttagctggtg tcaatcgtcc acagaatcct gatgaattca tggagggaaa
     8281 ttacggtttt tcaagtagat tattggagat tttagaaaag tatgaaaaca cttgtcctgt
     8341 tctactctca agttctactc aagctagttt agaaggccga ttttcaaact ctatatatgg
     8401 acaatctaag ctagcagggg aagaactctt ctttgaatat ggaaagaaaa cgggagcacc
     8461 tgtcttagtt taccgtttcc cgaatcttta tgggaagtgg tgccgtccta actacaattc
     8521 tgctgtagca actttctgtt ataatctagc tcacgattta cctattcaag taaatgatcc
     8581 aagtgtagaa ttggagttgc tgtatattga tgatttgata caagagtgtc taactgcatt
     8641 ggaaggaaat cctcatcgtt gtaatctaga tggattacaa atcttaccta gcccatcagg
     8701 aaactactgc tacgtaccaa cgactcatcg tgcaacctta ggagagattg tctctctatt
     8761 agaaacattt aaaaaacagc ctgatagttt agttatgcct gaaattcctc agggatcgtt
     8821 taaaaagaaa ttgtattcta cctacctatc ttatttacca gtagataagt ttaagtttcc
     8881 tctaaaaatg aatatagatg aacgaggtag ttttacggaa ttattaaaaa cagaaaatac
     8941 gggtcaattt tctgttaata tttctaaacc tggcattacc aaagggcaac attggcatca
     9001 ttctaagtgg gaatttttca tggttgtttc tggtcgtgct ttaatacaag agcgtaggat
     9061 agggctggat gaaaacggtc aagagtatcc tattctgaac tttgaagtgt cgggcgataa
     9121 gattgaagca atacatatga taccgggcta tgcacataat attattaatc tttctgatac
     9181 agaaaatcta attactgtca tgtgggctaa tgagtcattt gatcctagac atccagatac
     9241 tttttttgaa caagtggaga aataaatgaa aattaagaca gattatagtg atattcactt
     9301 taaagataat ggcaaactta agttattgat tattgtggga acacgtccag agattatacg
     9361 tctaagtagt gttatcacta aatgtcgaaa gtattttgat gttattttgg cacatactgg
     9421 acaaaactat gattacaatt taaatggtat tttctttgat aatttagggt tagacactcc
     9481 ggacgtatac atggatgctg ttggagatga tcttggtgct actgtaggga atattattaa
     9541 tacttcatac aaattgatga atcaaattaa accagatgct ttattgattt taggggatac
     9601 aaattcttgt ttatcagcta ttgctgccaa gcgtttacat attccaattt ttcatatgga
     9661 ggctggcaat cgctgtaaag atgagtgcct accggaagag actaatcgtc ggattgttga
     9721 tgttatttca gatgttaact tagcatactc tgaacatgca cgtaagtatt tacatgagtg
     9781 tggtttacct aaagagcgca catatgtaac tggttctcct atggcagaag tgttacataa
     9841 aaatttatct gctattgagt cttcagatat ccatgaacgt ttgggattga aaaaaggagg
     9901 ttatatctta ctttcagctc accgtgagga aaatattgat acagataaaa attttatttc
     9961 tctctttaca gcaattaatc aattagctga aaagtataat atgccaatct tatattcttg
    10021 ccatcctaga tcaaagaaaa ggctacaaga gagcggtttt aaactagata aacgtgtgat
    10081 tcagcatgag ccactaggat tccatgacta taattgttta cagatgaatg catttgttgt
    10141 agtatctgat tcgggaactt taccggaaga aagtagtttc tttactagtc aaggttatcc
    10201 ttttcccgct gtatgtattc gtacaagtac agaacgtcct gagtccttag ataaggcagg
    10261 atttattttg gcaggcattg atgaaaattc tcttcttcaa gcagttgaaa ctgctgttag
    10321 cttggcacaa gatgaggatt ttggattacc ggttccggat tatgttgagg aaaatgtctc
    10381 tactaaagtt gttaagataa tacagagcta tacagggata gtggataaaa tagtttggag
    10441 gaaaagctga attgtatatt tttaataaat caaaacgaga tgattaattc ttagtagaac
    10501 aagagtaaaa ttgaacgtat ttgatggaaa tgtcttactt gatagtcgag ggatagccat
    10561 tctatcggat ttttggcttg tttttgcgct tcatgtttga tagaataaag ggctctttgt
    10621 caactgtagt gggttgaaga aaagctaagc ttgagaaagg acaaatttcg tcctttcttt
    10681 tttgatgttc agagcgataa aaatccgttt tttgaagttt tcaaagtttc gaaagtaacc
    10741 gcccaataac gaagtctatt gaaaaatctc cagactagag aactcatata tagttcctaa
    10801 tctggagatt tcttatttgc acttttcttg tacaacttta gtccacggta aataggcctc
    10861 taaaacctct ttgtttacga gagtttcctc gtttggaaga cattctagaa gataggatag
    10921 atatttctcg ctatttataa tggattgtaa taagatatga acaaatcgat taggaaagga
    10981 aaattgattt atagaaatat tttagcagtc gatgcgtact gttatagatt caacgcacta
    11041 taattgatga cgtttagctg tttccaacaa actcataata atagctcttg ctttagctcc
    11101 ttcaaaactt tgagaaaata accaccctcg cttttttaaa aataaccacc ctcgtttttt
    11161 taaactgagc ttaggctaaa agagtccact ggactctttt actccgtccc ataaccaatg
    11221 atttaatggc gcgttcagct agattattgg aaaggaccag atgtccgtct tttaaaatgg
    11281 tcttaaaggt ttcttcatac ttgaggctgt attcaattgc ccttcctagt tttgaacccg
    11341 ataaaactga ctgacgacgg caccaagcaa agaagtcttc cagtaggggt tggagatgtt
    11401 cttgacgttt ctgtagccgt tcatcagctg gcaaagcctc ccagtctctt tccaaggaaa
    11461 ataactgatc acaataggct aaacctttag ctcctaagga tgatttatct gcttgcttgg
    11521 ggggcacttc aaaaaacttc cttctcacat gcgcccaaca tccaacaagt gcagcttctt
    11581 ccagttgacg ataagcttct agctgttcgc agtttaccaa gcttagcgtc gccttactcc
    11641 taaaccttgg actgctatcg cataggcaga actagaggac taaagtccta agttactgcc
    11701 gcaacatatc acaatgaaca tagccagaat aatctcctag gaattcttgt actactgaac
    11761 cacttcgaca ctgatcatgg tggtaaagcg taatcccttg tttctctgct ttacctgaca
    11821 aaaaagtcca atagtaggtc agatggctat cactctctag aacccgatag gaggtttcat
    11881 ccgcatgaag taagggctga gtcaatagtc tctctcgcaa gaggttataa aggggcttta
    11941 ggaataggag ctttcacaag cttatccaga tgattatctt ttactcgtta tggacaatgc
    12001 tatatggcat aaatcaagta ccttaaagat tccgactaat attggctttg catttattcc
    12061 tccatacaca ccagagatga accccattga acaagtgtgg aaagagattc gtaaacgtgg
    12121 atttaagaat aaagcctttc gaactttgga agatgtcatg aatcaacttc aagatgttat
    12181 acaaggactg gagaaggagg tgataaagtc catcgttaat cggagatgga ctagagtgct
    12241 ttttgaaagc agatgagtat aaaaagaaag tcctcatttc aatagaaatc acgactttct
    12301 gatggattta tagtaaaatg aaataataac aggacaaatc gatcaggaca gtaaaatcga
    12361 tttctaacaa tgttttagaa gcagaggtgt actattctag tttcaatcta ctatattttt
    12421 ggagtgatag aaaagccttt catcagccaa tctacttgtt caggtgcgag agctttgaca
    12481 tccttttctg tactgggcca agtcagtctg ccgttctcaa agcgtttata tagtagccaa
    12541 aatccttgac catcccagta aaggacttta aagcggtctt tacgtccacc acaaaagaga
    12601 aagatttgac cggagaaagg atccaattca aagtgggttt taacgagata agccagtgaa
    12661 tcgattcctt gtctcatatc agttttccca cacacgagat agacctgccc taggctagat
    12721 agatgaattg tcatagagca acaccttatc caaaagctgt tctatcattt cgagattgag
    12781 agattgaaaa aaacttactt caagcttgcc aatacgaatt ttaagtagaa tatcatttct
    12841 ctttttagaa tcaaaacgac gagattgtgg aatctctaca ggaacaatag gttgtggcat
    12901 taaaatatcc tccaatattt actttactaa tagtatacag gaggagctcc atgaatgata
    12961 gataccttgt tatgacgcgc ttacgatatt catatttttt gcaaagatgt tgtttgaaaa
    13021 ataattttca aaaattctga aaattctgtt gacaactttc tgaaaagagt ctataatgga
    13081 gagaaagttt taaaggagaa aatgatgaaa agttcaaaac tatttgccct tgcgggcgtg
    13141 acattattgg cggcgactac tttagctgca tgctctggat caggttcaag cactaaaggt
    13201 gagaagacat tctcatacat ttatgagaca gaccctgata acctcaacta tttgacaact
    13261 gctaaggctg cgacagcaaa tattaccagt aacgtggttg atggtttgct agaaaatgat
    13321 cgctacggga actttgtgcc gtctatggct gaggattggt ctgtatccaa ggatggattg
    13381 acttacactt atactatccg taaggatgca aaatggtata cttctgaagg tgaagaatac
    13441 gcggcagtca aagctcaaga ctttgtaaca ggattaaaat atgctgctga taaaaaatca
    13501 gatgctcttt accttgttca agaatcaatc aaagggttgg atgcctatgt aaaaggggaa
    13561 atcaaagatt tctcacaagt aggaattaag gctctggatg aacagacagt tcagtacact
    13621 ttgaacaaac cagaaagctt ctggaattct aagacaacca tgggtgtgct tgcgccagtt
    13681 aatgaagagt ttttgaattc aaaaggagat gattttgcca aagctacgga tccaagtagt
    13741 ctcttgtata acggtcctta tttgttgaaa tccattgtga ccaaatcctc tgttgaattt
    13801 gcgaaaaatc cgaactactg ggataaggac aatgtgcatg ttgacaaagt taaattgtca
    13861 ttctgggatg gtcaagatac cagcaaacct gcagaaaact ttaaagatgg tagccttaca
    13921 gcagctcgtc tctatccaac aagtgcaagt ttcgcagaac ttgagaagag tatgaaggac
    13981 aatattgtct atactcaaca agactctatt acgtatctag ttggtacaaa tattgaccgt
    14041 cagtcctata aatacacatc taagaccagc gacgaacaaa aggcatcgac taaaaaggct
    14101 ctcttaaaca aggatttccg tcaggctatt gcctttggat ttgaccgtac agcctatgcc
    14161 tctcagttga atggacaaac tggagcaagt aaaatcttgc gtaatctctt tgtgccacca
    14221 acatttgttc aagcagatgg taaaaacttt ggcgatatgg tcaaagagaa attggtcact
    14281 tatggggatg aatggaagga tgttaatctt gcagattctc aggatggtct ttacaatcca
    14341 gaaaaagcca aggctgaatt tgctaaagct aaatcagcct tacaagcaga aggagtccaa
    14401 ttcccaattc atttggatat gccagttgac caaacagcaa ctacaaaagt tcagcgcgtc
    14461 caatctatga aacaatcctt ggaagcaact ttaggagctg ataatgtcat tattgatatt
    14521 caacaactac aaaaagacga agtaaacaat attacatatt ttgctgaaaa tgctgctggc
    14581 gaagactggg atttatcaga taatgtcggt tggggtccag actttgccga tccatcaacc
    14641 taccttgata ttatcaaacc ttctgtagga gaaagtacta aaacatattt agggtttgac
    14701 tcaggggaag ataatgtagc tgctaaaaaa gtaggtctat atgactacga aaaattggtt
    14761 actgaggctg gtgatgagac tacagatgtt gctaaacgct atgataaata cgctgcagcc
    14821 caagcttggt tgacagatag tgctttgatt attccaacta catctcgtac agggcgtcca
    14881 atcttgtcta agatggtacc atttacaata ccatttgcat tgtcaggaaa taaaggtaca
    14941 agtgaaccag tcttgtataa atacttggaa cttcaagaca aggcagtcac tgtagatgaa
    15001 taccaaaaag ctcaggaaaa atggatgaaa gaaaaagaag agtctaataa aaaggctcaa
    15061 gaagatctcg caaaacatgt gaaataactg ttgcaaaata taagaaagga tttagtattt
    15121 cccttgaatg ctgaatcctt ttttacattt gtaaagaaag attctaaaat gtacggaccc
    15181 ccaaaagttg gagcctcttt ttgtcagaat agagaaaatt tttgttaatt ttacttgttt
    15241 cctattgctt tctcagctat tatttgttat attaaaagta taattatttt ttatttatca
    15301 gagttaagca ttgcactttc agaggaagga gtatttttta aaaagaaaat gtaaacgttt
    15361 gctcaaaaat gaaaggattt agaagtttat gaataaagga ttatttgaaa aacgttgtaa
    15421 atatagtatt cggaaatttt cattaggtgt tgcttctgtt atgattggag ctgcattctt
    15481 tgggacaagt ccggttcttg cagatagcgt gcagtctggt tccacggcga acttaccagc
    15541 tgatttagct actgctcttg caacagcaaa agagaatgat gggcgtgatt ttgaagcgcc
    15601 taaggtggga gaagaccaag gttctccaga agttacagat ggacctaaga cagaagaaga
    15661 actattagca cttgaaaaag aaaaaccggc tgaagaaaaa ccaaaagagg ataaacctgc
    15721 agctgctaaa cctgaaacac ctaagacggt aacccctgaa tggcaaacgg tagcgaataa
    15781 agagcaacag ggaacagtca ctatccgaga agaaaaaggt gtccgctaca accaactatc
    15841 ctcaactgct caaaatgata acgcaggcaa accagccctg tttgaaaaga agggcttgac
    15901 cgttgatgcc aatggaaatg caactgttga tttaaccttc aaagatgatt ctgaaaaggg
    15961 caaatcacgc tttggtgtct ttttgaaatt taaagatacc aagaataatg tttttgtcgg
    16021 ttatgacaag gatggctggt tctgggagta taaatctcca acaactagca cttggtatag
    16081 aggtagtcgt gttgctgctc ctgaaacagg atcaacaaac cgtctctcta tcactctcaa
    16141 gtcagacggt cagctaaatg ccagcaataa tgatgtcaat ctctttgaca cagtgactct
    16201 accagctgcg gtcaatgacc atcttaaaaa tgagaagaag attcttctca aggcgggctc
    16261 ttatgacgat gagcgaacag ttgttagcgt taaaacggat aaccaagagg gggtaaaaac
    16321 agaggatacc cctgctgaaa aagaaacagg tcctgaagtt gatgatagca aggtgactta
    16381 tgacacgatt cagtctaagg tcctcaaagc agtgattgac caagccttcc ctcgtgtcaa
    16441 ggaatacagc ttgaacgggc atactttgcc aggacaggtg caacagttca accaagtctt
    16501 tatcaataac caccgaatca cccctgaagt cacttataag aaaatcaatg agacaacagc
    16561 agagtacttg atgaagcttc gcgatgatgc tcacttaatc aatgcggaaa tgacagtacg
    16621 cttgcaagtt gtagacaatc aattgcactt tgatgtgact aagattgtca accacaatca
    16681 agtcactcca ggtcaaaaga ttgatgacga aagcaaacta ctttcttcta ttagtttcct
    16741 cggcaatgct ttagtctctg tttctagtaa tcaaactggt gctaagtttg atggggcaac
    16801 catgtcaaac aatacgcatg tcagcggaga tgatcatatc gatgtaacca atccaatgaa
    16861 ggatttggct aagggttaca tgtatggatt tgtttctaca gataagcttg ctgctggtgt
    16921 ttggagtaac tctcaaaaca gctatggtgg tggttcgaat gactggactc gtttgacagc
    16981 ttataaagaa acagtcggaa atgccaacta tgtaggaatc cacagctctg aatggcaatg
    17041 ggaaaaagct tataagggca ttgttttccc agaatacacg aaggaacttc caagtgctaa
    17101 ggttgttatc actgaagatg ccaatgcaga caagaacgtt gattggcaag atggtgccat
    17161 tgcttatcgt agcattatga acaatcctca aggttgggaa aaagttaagg atatcacagc
    17221 ttaccgtatc gcgatgaact ttggttctca agcacaaaac ccattcctta tgaccttgga
    17281 tggtatcaag aaaatcaatc tccatacaga tggtcttggg caaggtgttc tccttaaagg
    17341 atatggtagc gaaggccatg actctggtca cttgaactat gctgatattg gtaagcgtat
    17401 cggtggtgtc gaagacttca agaccctaat tgagaaggct aagaaatatg gagctcatct
    17461 aggtatccac gttaacgctt cagaaactta tcctgagtct aaatacttca atgaaaaaat
    17521 tctccgtaag aatccagatg gaagctatag ctatggttgg aactggctag atcaaggtat
    17581 caacattgat gctgcctatg acctagctca tggtcgtttg gcacgttggg aagatttgaa
    17641 gaaaaaactt ggtgacggtc tcgactttat ctatgtggac gtttggggta atggtcaatc
    17701 aggtgataac ggtgcctggg ctacccacgt tcttgctaaa gaaattaaca aacaaggctg
    17761 gcgctttgcg atcgagtggg gccatggtgg tgagtacgac tctaccttcc atcactgggc
    17821 agctgacttg acctacggtg gctacaccaa taaaggtatc aacagtgcca tcacccgctt
    17881 tatccgtaac caccaaaaag atgcttgggt aggggactac agaagttatg gtggtgcagc
    17941 caactatcca ctgctaggtg gctacagcat gaaagacttt gaaggctggc agggaagaag
    18001 tgactacaat ggctatgtaa ccaacttatt tgcccatgac gtcatgacta agtacttcca
    18061 acacttcact gtaagtaaat gggaaaatgg tacaccggtg actatgaccg ataacggtag
    18121 cacctataaa tggactccag aaatgcgagt ggaattggta gatgctgaca ataataaagt
    18181 agttgtaact cgtaagtcaa atgatgtcaa tagtccacaa tatcgcgaac gtacagtaac
    18241 gctcaacgga cgtgtcatcc aagatggttc agcttacttg actccttgga actgggatgc
    18301 aaatggtaag aaactttcta ctgataagga aaagatgtac tacttcaata cgcaggccgg
    18361 tgcaacaact tggacccttc caagcgattg ggcaaagagc aaggtttacc tttacaagct
    18421 aactgaccaa ggtaagacag aagagcaaga actaactgta aaagatggta aaattaccct
    18481 agatcttcta gcaaatcaac catacgttct ctatcgttcg aaacaaacta atcctgaaat
    18541 gtcatggagt gaaggcatgc acatctatga ccaaggattt aatagcggta ccttgaaaca
    18601 ttggaccatt tcaggcgatg cttctaaggc agaaattgtc aagtctcaag gggcaaacga
    18661 tatgcttcgt attcaaggaa acaaagaaaa agttagtctc actcagaaat taactggctt
    18721 gaaaccaaat accaagtatg ccgtttatgt tggtgtagat aaccgtagta atgccaaggc
    18781 aagtatcact gtgaatactg gtgaaaaaga agtgactact tataccaata agtctctcgc
    18841 gctcaactat gttaaggcct acgcccacaa tacacgtcgt gacaatgcta cagttgacga
    18901 tacaagttac ttccaaaaca tgtacgcctt ctttacaact ggagcggacg tctcaaatgt
    18961 tactctgaca ttgagtcgtg aagctggtga tcaagcaact tactttgatg aaattcgtac
    19021 ctttgaaaac aattcaagca tgtacggaga caagcatgat acaggtaaag gcaccttcaa
    19081 gcaagacttt gaaaatgttg ctcagggtat cttcccattt gtagtgggtg gtgtcgaagg
    19141 tgttgaagat aaccgcactc acttgtctga aaaacacaat ccatatacac aacgtggttg
    19201 gaatggtaag aaagtcgatg atgttatcga aggaaattgg tcactcaaga caaatggact
    19261 agtgagccgt cgtaacttgg tttaccaaac catcccacaa aacttccgtt ttgaagcagg
    19321 taagacctac cgtgtaacct ttgaatacga agcaggatca gacaatacct atgcttttgt
    19381 agtcggtaag ggagaattcc agtcaggtcg tcgtggtact caagcaagca acttggaaat
    19441 gcatgaattg ccaaatactt ggacagattc taagaaagcc aagaaggcaa ccttccttgt
    19501 gacaggtgca gaaacaggcg atacttgggt aggtatctac tcaactggaa atgcaagtaa
    19561 tactcgtggt gattctggtg gaaatgccaa cttccgtggt tataacgact tcatgatgga
    19621 taatcttcaa atcgaagaaa ttaccctaac aggtaagatg ttgacagaaa atgctctgaa
    19681 gaactacttg ccaacggttg ccatgactaa ctacaccaaa gagtctatgg atgctttgaa
    19741 agaggcggtc tttaacctca gtcaggccga tgatgatatc agtgtggaag aagcgcgtgc
    19801 agagattgcc aagattgaag ctttgaagaa tgctttggtt cagaagaaga cggctttggt
    19861 agcagatgac tttgcaagtc ttacagctcc tgctcaggct caagaaggtc ttgcaaatgc
    19921 ctttgatggc aatgtgtcta gtctatggca tacatcttgg aatggtggag atgtaggcaa
    19981 gcctgcaact atggtcttga aagaaccaac tgaaatcaca ggacttcgct atgttccgcg
    20041 tggatcaggt tcaaatggta acttgcgaga tgtgaaactt gttgtgacag atgagtctgg
    20101 caaggagcat acctttactg caactgattg gccaaataac aacaaaccaa aagatattga
    20161 ctttggtaag acaatcaagg ctaagaaaat tgtccttact ggtaccaaga catacggaga
    20221 tggtggagat aaataccaat ctgcagcgga acttatcttt actcgtccac aggtagcaga
    20281 aacacctctt gacttgtcag gctatgaagc agctttggtt aaggctcaga aattaacaga
    20341 caaagacaat caagaggaag tagctagcgt tcaggcaagc atgaaatatg cgacggataa
    20401 ccatctcttg acggaaagaa tggtggaata ctttgcagat tatctcaacc aattaaaaga
    20461 ttctgctacg aaaccagatg ctccaactgt agagaaacct gagtttaaac ttagatcttt
    20521 agcttccgag caaggtaaga cgccagatta taagcaagaa atagctagac cagaaacacc
    20581 tgaacaaatc ttgccagcaa caggtgagag tcaatctgac acagccctca tcctagcaag
    20641 tgttagtcta gccctatctg ctctctttgt agtaaaaacg aagaaagact agtatttagt
    20701 aaaacctctt aacaagatta cggaagcagt ctctatcttt tccaatgagg tttatagtac
    20761 agaaaaagcc tgagaagatg tcttctcagg cttttgttaa gcacataaat acaatagtgc
    20821 tatgacaaaa tcacccagaa aaatctgggt gataaatgtt atggttgtgc tggttgagga
    20881 ttctgatttt gttgatcagg ggttgtattt gattgttgcg tattattgtt aggattggta
    20941 gtcgtactat tatttgtgct tggagtggtt gagctagact gtgaagttga actatctgat
    21001 gatgagcttg aactttcagt tgatgggggt tgttgtggag caggtgagtt ccacgtagaa
    21061 cgagcaccat ttttaaatac gaattctcca tttctgtaga gcccctctgg tatattccaa
    21121 tcttctggat tgcttccttc agacaggtag gtcatcatag agcggtaaac tttggcagcg
    21181 accgtaaggc cattgcctac aagtggtgtc agacggttag aatagcctgt ccatacagcc
    21241 attgaatatt tacgcgtata gccagcaaat agttcatcag gtgctacaaa ttgagaggtc
    21301 ttgatgtggt tttcaatttc ctcgtctgta tagttagagg ttcctgtttt accagcctga
    21361 gggagccaag caagataggc atttcgtcca gttccataag tcaagactgt tttcatcatg
    21421 tcggtcatca tataggctgt cgtttccttc atggcacgag ttccgacatt agagaactct
    21481 ttttcactcc catcactaaa gacgacttta tggatataca ttggtttata gtaagttcca
    21541 ccatttgcaa aggcagcgta agcagcagcc atcttttcac tacttgctcc atattttttg
    21601 tctgattcgg ttgtgttact tgaaatggca tttgagtagt gaatacttgg gtagtcgatt
    21661 cctagaccat ttaggaaagt cttggcgcgg ttgagtccga ccttgtttag agtttccacg
    21721 gctgggacgt ttcgcgattg ttgcagggcg tattgcaagg tgatgttgcc aaagtagccc
    21781 ctatcccagt tataaacagg agtatttgtc ccagggtagt tatagggctc atcgtgaacg
    21841 atagtagcag ttgaatcgta gacaccgtac tccaaggcag gagcatagtc tgtgatcggt
    21901 ttcatagttg atccccagtc gcggtttgtt tctactgctt ggttaattcc gaaggaaaca
    21961 ttacttgact gatggcgtgc tcctagctgg gcaatgactt taccgttaga aacatcaaca
    22021 atggtagaag cgacttgcaa ttcatcgtct ggataggcaa cgtattcgtc tgtattgtaa
    22081 atatcccaca gatgtttttg agcttcttgg tctacatttg tgtagacatc catcccagtt
    22141 gtgagtaggt tatagcctgt ttcttcttca acttgattga tgacttcctt gaggtaatta
    22201 tccatgtaag cagggtaatt acttgctgat ttgagacttt gtagtccatc agtaattggt
    22261 gtattgactg ctttctcata ctgttcagca gagatgtagc cttgattttt catttcagat
    22321 aagaccaagt ttcggcggtc ttgggctgct tctggatgtg aataggggtc atattggttt
    22381 ggtgcctgag gcattccagc cagcaaggct aactgaggta aacttaaatt attgaggtct
    22441 ttaccatagt agttttgagc tgctgtctgc attccatagt tcccattaga catgtagacc
    22501 ttatttatat agtaggtcaa gatttcttgc ttggttgctt tttgttctaa ctgaatcgct
    22561 aaccaagctt cctgagcctt acgagaaata gtctggtcgg aagtcgaagt tgaaaagtaa
    22621 gtcaacttaa tcaactgttg ggtgagagtt gatccacctt ggagggaatt gctttgcaga
    22681 ttgcgcaaga aagctcccag gatacggatg gtatcaatcc ccctgtggtc gaagaagcga
    22741 tggtcttcga tagaaacgat tgccttaacc aaatctgtgg gaatatcatt agcttgggca
    22801 ttgacgcggc gttcagaacc caagtcagca atgagttgat ttttattgtc gtagatttta
    22861 ctagaagttg ttgcaactag tttactctcg gataggctag gagccttgct aacgtagtag
    22921 aaaaaaactc ctccgcctaa gacaatggct gcgataacca agcttaagaa gctaatgctc
    22981 agatacttga ttaggcgcag aatcgttggt ttgttcatct tgttttacca cctaataaat
    23041 gttctttgat aacattgaga taaggaattt gagggaaggc accagccttg atttcatatc
    23101 catattctcg aatatattca agtggcattg atttttgtcc cttatcttga tgatagaagc
    23161 gaatcaaatc gaatgccggc aataagtagg tttcttgctg agaagaaaag tgaagaagga
    23221 caaagcagat tccttgttgg gcaaggactt gttccatatg ctgaatctga tgtggatgaa
    23281 aatttttcat cggaatcgca cgtttttgtt ttgtttcctt gacttcaaag tcgatgtaat
    23341 atccattata aacgccagaa tagtccgtcg ttgaagcttg tcgaaaatag gcttcaacaa
    23401 tcttggcacg acttcgttgt ggatagtcca cttgtacgat ttgaatagga gttggtttct
    23461 tatgtataac agccaagccc tgagacaaat agtagtcgtt ggtagcattg atcatctttt
    23521 caaaagacat tcctcgattt gcgaaatttt tgggttgaga aagagatgtt tgtctttttt
    23581 gtgatgaaac tttatgtgga tagttgacca taattctcct tattggtaca ataacatcac
    23641 tttattatac cataatttgc acagaaaggg ttaaaaatgg ctacagcttt ggttttaggc
    23701 tattctgctt ttgatttggg tttattttct gataaggatc ctcgtcttaa attgattaaa
    23761 aaagcgatac gtaaagattt agaggctatg gcagcagatg gggtgtcttg gcttgtgttt
    23821 acagggagtt tgggttttga atattgggtt ttagaggttg ctcaggagat gaaaactgaa
    23881 tacggtttcc agttggccac catttttgct tttgaaaccc atggggaaaa ttggaatgaa
    23941 ggcaatcaga tgaaactgag tcgctttaag caggtagact ttgtcaaata tgcctatcct
    24001 cgctatgaac acaaggggca gttaagagat taccagcagt ttttgctgga aaacacgact
    24061 agttcctatc ttttttatga tgaagaaaat gaaacaaaac tagcttattt ttaccaaaag
    24121 atgaaaaatc aagaggacta ttttataaag agattaacat ttgatgaatt aaatgaactt
    24181 gctgaaaatt tttccgaaaa ttgaagcttt gaccttgatt tttgtttgcc tttttttata
    24241 taataatact agcaagcgag aatggagaga gacatggcaa gtattatttt ttcagcgaaa
    24301 gatatttttg aacaagagtt tggacgtgag gtccgtggct ataataaagt agaagttgac
    24361 gagtttttag acgatgtcat caaggactat gaaacctatg ctgccttggt caagtcactt
    24421 cgtcaggaaa ttgcggattt gaaggaagaa ttaactcgta aaccgaaacc ttcaccagtt
    24481 caagcagaac cccttgaagc ggcaattaca agttctatga cgaattttga tattttgaaa
    24541 cgcctgaata gattggaaaa agaagttttt ggtaaacaaa ttttagataa ctcagatttt
    24601 taagtagtta tttgagatgt gcaatttttg gataatcgcg tgaggagaat tgtttctcat
    24661 gaggaaagtc catgctagca caggctgtga tgcctgtagt gtttgtgcta ggcgaaacca
    24721 taagcctagg gacgagaaat cgttacggca gttgaaatgg ctaagtcctt ggataggcca
    24781 gagtaggctt gaaagtgcca cagtgacgga gtctttctgg aaacagagag agtggaacgc
    24841 ggtaaacccc tcaagctagc aacccaaatt ttggtcgggg catggagtac gcggaaacga
    24901 acgtagtatt ctgactgcta tcagctagag ctgttagtgg tagacagatg attatcgaag
    24961 gaagtggtcc tagtcacttc tggaacaaaa catggcttat agaaaattgc atataggttg
    25021 gggctgagaa attttctcaa cctcattttt taaagtggac atatagaaag gtcttgcaag
    25081 actgtaacat gaaaaaagaa tttaatttaa ttgcaactgt ggcagcaggg cttgaggctg
    25141 tcgttggtcg tgaagtgcga gagttgggct acgattgtca ggttgaaaat ggacgtgttc
    25201 gttttcaagg agacgtgaga gctattatcg aaaccaacct ttggcttcgg gcagcagatc
    25261 gtatcaaaat tatcgtagga acgttcccag ctaagacttt tgaagagcta tttcagggag
    25321 ttttcgcttt ggattgggaa aattatttac cacttggagc tcggttcccg atttcaaaag
    25381 ctaaatgtgt taagtccaaa cttcacaatg agcccagtgt tcaggctatt tctaagaaag
    25441 ctgttgtcaa gaaattgcag aaacactatg ctcgcccaga aggggttcct ctgatggaga
    25501 atggcccaga gtttaagatt gaggtctcta ttctcaaaga tgtggcaact gtcatgattg
    25561 atacgaccgg gtctagcctc tttaaacgtg gttatcgtac cgaaaaaggt ggcgctccta
    25621 tcaaggaaaa tatggcagca gccattttac aactttctaa ctggtatcca gacaagcctt
    25681 tgattgatcc gacctgtggt tcggggactt tctgtattga ggcagttatg attgctagaa
    25741 agatggcgcc aggtcttcgt cgctcttttg catttgagga atggaactgg atcagcgatc
    25801 gcttgattca agaagtgcgc acagaagcgg ctaaaaaagt agaccgtgag cttgagctgg
    25861 atatcatggg ctgtgatatt gatgctcgca tggtggaaat tgctaaggcc aatgctcagg
    25921 tagctggtgt tgcaggagac attactttta agcagatgcg cgtgcaggat ttacgttccg
    25981 ataaaatcaa tggagtaatc atttccaatc cgccttatgg tgaacgtttg tcagatgatg
    26041 caggggtgac caagctctat gctgagatgg ggcaagtatt tgcaccgctg aaaacttgga
    26101 gcaaatttat cctgactagt gatgaagctt ttgaaagcaa gtatggtagc caagcagata
    26161 agaagcgtaa gttatacaac ggaaccttga aagtggatct atatcaatat tttggtcagc
    26221 gtgtcaaacg gcaagaggta aaatagaaag ggatactcat gagtaaaaaa agacgaaatc
    26281 gtcataaaaa agaaggtcaa gaaccgcaat ttgattttga tgaagcaaaa gagctaacag
    26341 ttggtcaagc tattcgtaaa aatgaagaag tggaatcagg agtcttgcct gaggattcca
    26401 ttttggacaa gtatgttaag caacacagag atgaaattga ggcggataag tttgcgactc
    26461 gtcaatacaa aaaagaggag ttcgttgaaa ctcagagtct ggatgattta attcaagaga
    26521 tgcgtgaggc tgtagagaag tcagaagctt cttcggagga agttccatct tctgaagaca
    26581 tcttactacc cttgcctctg gacgatgagg agcaaggctt ggatcctcta ttgctagatg
    26641 atgaaaatcc aacagaaatg actgaagaag tggaagagga gcaaaacctt tctcgtctgg
    26701 atcaagagga ctcagaaaag aaaagtaaaa aaggctttat tttgaccgtt ttggcgcttg
    26761 tatcagtaat tatttgtgtc agtgcttatt atgtctaccg tcaagtggct cgttcgacta
    26821 aggaaattga aacttctcaa tcaactacag ccaatcaatc ggatgtggat gattttaata
    26881 cactttatga cgccttttac acagatagca ataaaacggc tttgaaaaat agccagtttg
    26941 ataaactgag tcaactcaag actttacttg ataagctgga aggtagtcgt gaacatacgc
    27001 ttgccaaatc taaatatgat agtctagcaa cgcaaatcaa ggctattcaa gatgtcaatg
    27061 ctcaatttga gaaaccagct attgtggatg gtgtgttgga taccaatgcc aaagccaaat
    27121 cggatgctaa atttacggat attaaaactg gaaatacgga gcttgataaa gtgctagata
    27181 aggctatcag tcttggtaag agccagcaaa caagtacttc tagctcaagt tcaagtcaaa
    27241 ctagcagctc aagttcaagt caagcaagtt caaatacgac tagtgagcca aaaccaagta
    27301 gttcaaatga gactagaagt agtcgcagtg aagtcaatat gggtctctcg agtgcagggg
    27361 ttgctgttca aagaagtgcc agtcgtgttg cctataatca gtctgctatt gatgatagta
    27421 ataactctgc ctgggatttt gcggatggtg tcttggaaca aattctagcg acttcacgtt
    27481 cacgtggcta tatcactgga gaccaatata tccttgaacg tgtcaatatc gttaacggca
    27541 atggttatta caacctctac aagccagatg gaacctatct ctttaccctt aactgtaaga
    27601 caggctactt tgtcggaaat ggcgctggtc atgcggatga cttagattac taagcagtcg
    27661 ttacaaaatt ctttcttttc aaaagtaaaa atgataaaat aaaacaaatg aaacaagagg
    27721 agtgtcaaat gacaaaagct aactttggtg tcgtaggtat ggccgtaatg ggtcgtaacc
    27781 ttgcccttaa tattgaatct cgtggttaca cagttgctat ctacaaccgt agtaaagaaa
    27841 aaacggaaga tgtgattgct tgccatcctg aaaagaactt tgtaccaagc tatgacgttg
    27901 aaagttttgt aaactcaatc gaaaaacctc gtcgtatcat gctgatggtt caagctggac
    27961 ctggtacaga tgctactatc caagcccttc ttccacacct tgacaagggt gatatcttga
    28021 ttgacggagg aaatactttc tacaaagata ccatccgtcg taatgaagaa ttggcaaact
    28081 ctggtatcaa ctttatcggt actggggttt ctggtggtga aaaaggtgcc cttgaaggtc
    28141 cttctatcat gcctggtgga caaaaagaag cctacgaatt ggttgcggat gttcttgaag
    28201 aaatctcagc taaagcacca gaagatggca aaccatgtgt gacttacatc ggtcctgatg
    28261 gagctggtca ctatgtgaaa atggttcaca atggtattga gtacggtgat atgcaattga
    28321 tcgcagaaag ctatgacttg atgcaacact tgctaggcct ttctgcagaa gatatggctg
    28381 aaatctttac tgagtggaac aagggtgaat tagacagcta cttgattgaa atcacagctg
    28441 atatcttgag ccgtaaagac gatgaaggcc aagatggacc aatcgtagac tacatccttg
    28501 atgctgcagg taacaaggga actggtaaat ggactagcca atcatctctt gaccttggtg
    28561 taccattgtc actgattact gagtcagtgt ttgcacgcta catttcaact tacaaagaag
    28621 aacgtgtaca tgctagcaag gtgcttccaa aaccagctgc cttcaacttt gaaggagaca
    28681 aggctgaatt gattgaaaag atccgtcaag ccctttactt ctcaaaaatc atttcatacg
    28741 cacaaggatt tgctcaattg cgtgtagcct ctaaagaaaa caactggaac ttgccatttg
    28801 cagatatcgc atctatctgg cgtgatggct gtatcatccg ttctcgtttc ttgcaaaaga
    28861 ttacagatgc ttacaaccgc gatgcagatc ttgccaacct tcttttggac gagtacttct
    28921 tggatgttac tgctaagtac caacaagcag tacgtgatat cgtagctctt gcggttcaag
    28981 caggtgtgcc agtgccaact ttctcagcag ctattactta ctttgatagc taccgttcag
    29041 ctgaccttcc agctaacttg atccaagcac aacgtgacta ctttggtgct cacacttacc
    29101 aacgtaaaga caaagaagga accttccact actcttggta tgacgaaaaa taagtaggtc
    29161 agccatgggg aaacggattt tattacttga gaaagaacga aatctagctc attttttaag
    29221 tttggaactc cagaaagagc agtatcgggt tgatctggta gaggaggggc aaaaagccct
    29281 ctccatggct cttcagacag actatgattt gatgttattg aacgttaatc tgggagatat
    29341 gatggctcag gattttgcag aaaaattgag ccgaactaaa cctgcctcag tcatcatgat
    29401 tttagatcat tgggaagact tgcaagaaga gctggaagtt gttcagcgtt ttgcagtttc
    29461 atacatctat aagccagtcc ttatcgaaaa tctggtagcg cgtatttcgg cgatcttccg
    29521 aggtcgggac ttcattgatc aacactgcag tctgatgaaa gttccaagga cctaccgcaa
    29581 tcttaggata gatgttgaac atcacacggt ttatcgtggt gaagagatga ttgctctgac
    29641 acgccgtgag tatgaccttt tggcgacact tatgggaagc aagaaagtat tgactcgtga
    29701 gcaattgttg gaaagtgttt ggaagtatga aagtgcgacc gagacaaata tcgtagatgt
    29761 ctatatccgc tatctacgga gcaagcttga tgttaaaggc caaaaaagct acattaaaac
    29821 tgtgcgtggt gttggatata ccatgcaaga atagaaaagc agttgcagtt gtgtaactgc
    29881 ttttttgagg agtttt
//
LOCUS       DAVKNL010000025        26513 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVKNL010000025 DAVKNL010000000
VERSION     DAVKNL010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26513
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.025"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(86..647)
                     /locus_tag="VW411_001074"
                     /pseudo
     CDS             complement(86..647)
                     /locus_tag="VW411_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436408.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1195..2060
                     /locus_tag="VW411_001075"
                     /pseudo
     CDS             1195..2060
                     /locus_tag="VW411_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442363.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            2163..3857
                     /locus_tag="VW411_001076"
                     /pseudo
     CDS             2163..3857
                     /locus_tag="VW411_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268095.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            4157..4996
                     /locus_tag="VW411_001077"
     CDS             4157..4996
                     /locus_tag="VW411_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEU9793817.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKTGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGIIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTK
                     NLD"
     gene            5014..6852
                     /locus_tag="VW411_001078"
     CDS             5014..6852
                     /locus_tag="VW411_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9793818.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGAPVTPASYGSSVVPILIMV
                     WLMKYIEKIIAKLTLAITKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVEAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            6865..8280
                     /locus_tag="VW411_001079"
     CDS             6865..8280
                     /locus_tag="VW411_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU9793819.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWVDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEN
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDELNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGSGTLNRY
                     KKMSFTWYKEVIESNGESLFK"
     gene            8762..9808
                     /gene="pheS"
                     /locus_tag="VW411_001080"
     CDS             8762..9808
                     /gene="pheS"
                     /locus_tag="VW411_001080"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEU9793820.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            9808..10317
                     /locus_tag="VW411_001081"
     CDS             9808..10317
                     /locus_tag="VW411_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9793821.1"
                     /translation="MIRKVEMADVEVLAKIAKQTFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            10394..12799
                     /locus_tag="VW411_001082"
     CDS             10394..12799
                     /locus_tag="VW411_001082"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEU9793822.1"
                     /translation="MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQINVGEEEERQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMCGVAHEVAAIYDKAVNFKKFTLTETNEAAADALSVSIETDK
                     APYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDT
                     FEGTDIRVREARDGEKLVTLDGEERDLAETDLVITVADKPVALAGVMGGQATEISEKS
                     SRVILEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASMIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VSVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTVIQKLRRQVRTIAE
                     GAGLTEIITYALTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFTRLGLQVTYTATSEIVSLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSAIEGALQPAVPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNA
                     EVR"
     gene            complement(12867..13868)
                     /locus_tag="VW411_001083"
     CDS             complement(12867..13868)
                     /locus_tag="VW411_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU9793823.1"
                     /translation="MIKTFLSALSVILFSIPIITYSFFPSSNLNIWLSTQPILAQIYA
                     FPLATATMAAILSFLFFFLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NALPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            14024..14439
                     /locus_tag="VW411_001084"
                     /pseudo
     CDS             14024..14439
                     /locus_tag="VW411_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            14565..14831
                     /locus_tag="VW411_001085"
     CDS             14565..14831
                     /locus_tag="VW411_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9793824.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGKTSPQLDTVLKVLASLGKTLAIVPLEQGKS"
     gene            15092..17341
                     /gene="metE"
                     /locus_tag="VW411_001086"
     CDS             15092..17341
                     /gene="metE"
                     /locus_tag="VW411_001086"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEU9793825.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVAAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEANRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            17405..18271
                     /gene="metF"
                     /locus_tag="VW411_001087"
     CDS             17405..18271
                     /gene="metF"
                     /locus_tag="VW411_001087"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEU9793826.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNDNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQTPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            18956..21169
                     /gene="pnp"
                     /locus_tag="VW411_001088"
     CDS             18956..21169
                     /gene="pnp"
                     /locus_tag="VW411_001088"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEU9793827.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQVVKDQVT
                     AVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHQKDYKPKKEFTETSKDSE"
     gene            21185..21802
                     /gene="cysE"
                     /locus_tag="VW411_001089"
     CDS             21185..21802
                     /gene="cysE"
                     /locus_tag="VW411_001089"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEU9793828.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KHGFKLLARMYSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            21814..22698
                     /locus_tag="VW411_001090"
     CDS             21814..22698
                     /locus_tag="VW411_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9793829.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKAGQVIGTCTVDLSTNT
                     NYLYGLAILEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            22780..24123
                     /locus_tag="VW411_001091"
     CDS             22780..24123
                     /locus_tag="VW411_001091"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU9793830.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            24116..24502
                     /locus_tag="VW411_001092"
     CDS             24116..24502
                     /locus_tag="VW411_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEU9793831.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            24506..25390
                     /locus_tag="VW411_001093"
     CDS             24506..25390
                     /locus_tag="VW411_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEU9793832.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            25527..25982
                     /locus_tag="VW411_001094"
     CDS             25527..25982
                     /locus_tag="VW411_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793833.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGYNFSSVSSS
                     SQTAKYEGTVYNNNSSLYITIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNAK
                     DNAGNWTSASNKKEVTVKIVSAKPTYPDKILVKNPDNIKDTEKMPLLKN"
     gene            25979..26125
                     /locus_tag="VW411_001095"
     CDS             25979..26125
                     /locus_tag="VW411_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793834.1"
                     /translation="MKEANKNHPAGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVT
                     KYAR"
     gene            26268..26438
                     /locus_tag="VW411_001096"
                     /pseudo
     CDS             26268..26438
                     /locus_tag="VW411_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         8162 a         4675 c         5831 g         7845 t
ORIGIN      
        1 tgaaagcgat caagaacgat tttagcattc gggagtgaaa cagtctggga gactgtttca
       61 gcctgagcct agaaatttga aagcgaagct gtttagctaa gtcatagtaa ggactaaaca
      121 tatccatagt aataattttg acgcgacatc ggacggctct atcatattta agaaagtgat
      181 ctcggatgac agcttgtgtt ctaccctcaa gaacagtgat gatatcgagc ttttcaaaat
      241 cttgcgcaat aaagctcatc tccatctccc gattgaaaca gtcactcccc ggactgtttc
      301 aacgtcccaa gacataatct taggcagatt tctaaaatta cactcaaagt ggaagtcatt
      361 gagctttcga atgacagttg aagttgaaat ggccagctga tgggcaatat cggtcataga
      421 aatcttttca attaactttt gcgcaatctt ttggttgata atacgaggaa tttggtgatt
      481 tttcttgacg atagaagttt cagcgaccat cattttcaag caatgatagc acttaaaacg
      541 acgttttcta aggagaattc tagtaggcat accagtcgtt tcaaggtaag gaattttata
      601 gggtctttaa tgtctagtaa ttttgtgata aaatgtaatt gttccatatg attctttcta
      661 atgagttgtt ttgtcgcttt tcattataga tcttatggga ctttttttct acccaaaata
      721 ggctccataa tatccatagg gaatttaccc actacaaata ttatagagcc caaagtttta
      781 ggtcgcttga taatatgcgt tttttgaatt ttatagactg ctcgtttaaa ctctatttac
      841 ttcgtacctt ctggagcgag acggaatatt agtcacatac aaaatgagta ctattaggat
      901 tttattttca tgtacaattt cagccagtct tgttataatc agcctatagg aatcaaggag
      961 gtgactctta tggctgtttt tgtgtctttg gatggaattg tggtagaagt ccttgatgtc
     1021 ttttcttctt ttaatgggga tagtgagttt ttcttgtgta tagcattttg aatctggaat
     1081 aggacgccat gactgctaaa agatttctat aaattaattt gattttccta atcaatttgt
     1141 tcatatctta tttcattcca ctataaacgt cttaaagaca agagtcagtt tgttatggaa
     1201 cgctctcagt tcgaggagat gttccaactt caaagtagtc gcttgacgac gcaagaaaaa
     1261 ttacaattgt ttacctctgt gtttgctggc cgttatgatg tttatgctaa gaattttatc
     1321 aatgaacaag ggaaaattca gtattttcct tcctatgatt atggttggaa gcagttgcca
     1381 cctgaaaaac ggagtttcca gacattgacg aactccgttt tgaaatctca ttttcgtggg
     1441 gaggcagcta tcggtatctt tcctatgcac ttagatgata gctgttattt tttggtactg
     1501 gatttggatg aaggagattg gaaagaagct ggtttaacca ttcgaagaat agccagggaa
     1561 cgccagatgg aagcccattt agagatttct cgttcgggtc acggactcca tatttggttc
     1621 ttctttgagg aagcgattcc gagtcgagag gctcgcttgt ttggaaagaa actgatagaa
     1681 ctggcaatgc aggaaagtat gcaactgtcc tttgattctt ttgatcgcat gtttccaaat
     1741 caggatgtcc ttcctaaggg gggatttgga aatttgattg ccttgccttt tcaaggagaa
     1801 gcttaccatc aagggcgaac ggtctttgtg gatgaacagt ttcagcctta tgaagaccaa
     1861 tggaggtatc tacaagaaat tcagaggatt tcaactgcta aagtggcact gttaatccaa
     1921 gaggagttag gcaagcaaga attggataag gagttgaagg tcgttttatc caatatgatc
     1981 caacttgaaa aatcgtctgt gacatccaag gcactttttt cttgaaaaat atggcttcct
     2041 tttctaatcc cgaattttat agtagattga aactagaata gtacacctct gcttctaaaa
     2101 cattgttaga aatcgatttg actttcctga tcgatttgtc ctgttattat ttcattttac
     2161 tatatttaaa gcaggctatg cgacagccaa cctatcaaat tcctgagaga atgtatttat
     2221 ttggagaatc cgatcattat ttatggttgc caagaggttt gctgtatcca ttgcaagata
     2281 aatttaagca ggtatctgtg gaagatagga gaaaggtaca aaggtctatt agcgtggaat
     2341 ttaagggaga actcactttt gagcaagagt tagccctgtc agatatgact tctaaagaaa
     2401 atggtttact tcatgcggag actggttttg ggaagaccgt tttaggtgct gctcttatct
     2461 ctgaacggaa aacaaaaaca attattctag tccataataa gcaactctta gaccaatggc
     2521 tagatcgctt aaactgcttt ttgactttcg aagaggagga ggctatccgt tatacggcat
     2581 caggtcgtga aaaggtaatc ggctatgttg ggcagtacgg tgggactaag aaatggctga
     2641 gtaaactggt tgatgtcgtt atgattcaat ctctatttaa gttggaaaat agtcaaagtc
     2701 ttttggatga gtatgagatg atgattgtgg atgagtgtca tcatgtctct gccttgatgt
     2761 ttgaaaaagt tgttgctcag tttagaggga agtatcttta cggtttgacg gctacgcctg
     2821 agcgtaagaa tggtcatgag cctattgttt ttcagagaat tggtgagata ctccatactg
     2881 ctgataagag ggaaacggat tttaaacggc aattgcaatt aagattcact tcttttggtc
     2941 atttggaaat tgaaaagacc aaagcaagta attttataca gcttagtgat tggattgcta
     3001 ctgactcagt gaggaatcag atgattctca aggatattct agcccaagtg gcagaaggac
     3061 ggaatatctt ggttttagtt aatcgaattc aacagataga tgtctttgaa aaattattga
     3121 aagagaaaga ggttgatgac tgttacatta ttagcggaaa aaccaaagtc cgagagagaa
     3181 cgagtttact ggagacgtta gaacagttag ataaagggtt tgttttgttg tctactggaa
     3241 aatacattgg cgaaggtttt gacttacctc agttggacac gcttatcttg gcagcaccct
     3301 tttcttggaa aaataatttg attcagtatg caggtcggat tcatagaaac tacaaggata
     3361 agtctttggt gcgtattttc gattatgtgg gtattcatgt tccttattta gaaaagatgt
     3421 ttcagaaacg acaagtagct tatcgaaaga tggattatcg tgtcatcgag ggtgaggaga
     3481 aacaattcgt ttatgttgat agtagatatg agaaggtgtt gagagaggac ttagcagggg
     3541 aaagacagga atgtctgctt attttacctt atgtgcacca gacaaaactg atgaattttc
     3601 taaaagaatt taggattagt caaattgaga tatgtatacc agagacggtt gcaaataaag
     3661 catggctaga ccagttgaag agccagaaaa ttaaagtgtc ttttactcaa tcaaaaatag
     3721 taacgcctat tcttttggtg aataagacta ttgtttggta tggtgcaatg ccattattag
     3781 ggaaggtaga tgagatgacc atattacgtt tggaatcagc tagtatagtt tctgaactag
     3841 tggcaggttt acgatagaga aaatttttaa aaatttctat gtatgatttt catttcttta
     3901 gtgagactgt tgccattatc acattcgaat cacacaaaat aaaaaaattt ttataagtac
     3961 ttgacaaata gattgaaata tcataaaata aaaacggtta cagagttatt aattatttaa
     4021 gcttcatgtc accattaaaa attgaaataa aaggatgtta tcactaatac aagtgagcag
     4081 gaacctattt aatcacatca gaagaagttt cttgatgttt ttaagtaggt tccttttatt
     4141 ttaaaaggga aattttatga tcataaaacg aatactaaac cacaatgccg taattgcgca
     4201 aagtaaaaaa gatatcgata ttcttctttt tggaagggga atagcttttg gaagaaaaac
     4261 tggagataaa gtaaatccaa ttgatattga gaaaagtttt tttctcaaaa atagagataa
     4321 tatgacccgt tttacagaga tgtttattaa cgttcctttg gagttggtgt acatcaccga
     4381 aaaaataatt aacctaggta aaataacatt gggtaataat tttgatgaaa ttatctatat
     4441 taatttaacg gatcatattt cttcgagcat agaacgttat aaagaaggga ttattatttc
     4501 gaatccccta cgctgggaaa tatcgaaata ttataaagaa gaatttgaac ttgggaaaag
     4561 ggctttacaa ataataaaaa aagagttagg tattgaactt ccaattgacg aagctgcatt
     4621 catagcgcta cattttgtta atgctaattt agaaaataat tttcaagagt cgtataaaat
     4681 cactgaaata attatgggaa ttgagaaaat cattcaagat ttctattgta ctgagtttaa
     4741 ccaagattct attgattatt atagattcat aactcatata aaattatttg cccatcgctt
     4801 ggttgaaaat acaacttatt gtgacgatga tgatgaagac ttgttagcat taatgaaaaa
     4861 taaatatcct agagaatatg aatgtggtga acaggtagca atgtttatac aaacggagta
     4921 caattatttg ctgacttcta gtgaattagt ttatttgatg gctcatattc gtcgcttaac
     4981 aaaaaattta gattaataga ataggagaat accatgagtt ataaagatac ggtacaaaaa
     5041 atcctcgatg taattggagg tgaaaaaaat gtcaatagag ttacccattg tgtaacacgt
     5101 ttaagattag aattaaaaga tgaaaattta gtcaatgatg atgatgtgaa gaagatacca
     5161 ggtgtaatag gtattatgaa aaagaatgga caatatcaaa ttatacttgg taatgatgta
     5221 gctaattatt ataaagaatt cgttaaactt ggcaattttg aatccgattc agttgttcaa
     5281 gggcacaaag ggaatatttt agaaagaatc attgagtata tcgctggttc catgactcca
     5341 atcattccag caatgttagg gggaggtatg ttgaaagtct tggtaatcat tttaccaatg
     5401 cttggtatat tgcaatcaga ttctcagact attgcttttt tgacattttt tggggatgct
     5461 ccatattatt tcttaccgct gttattagct tattctgcat cacaaaaatt aaaagtaaca
     5521 tctacattag ctatgtctgt agcaggtgta cttctccatc caaattttgt tcaaatggtg
     5581 caatcaggga atcctcttag tttatttggt gcacctgtga caccagctag ttatggttca
     5641 tcagtcgttc caattcttat tatggtttgg ttgatgaaat atattgaaaa aataattgct
     5701 aaattaacac tagctattac taagagtttt ttgcaaccta cgctagtatt attagtatca
     5761 agctgtattg ccttagttgt agtcggacct attggagtaa ttgttggtga aggattatca
     5821 aatctagttg ggcaaatgta tggtgtagct ggatggctta cattagctat tcttggtgct
     5881 attatgccat ttattgttat gactggaatg cattgggctt ttgcacctat ttttttggcg
     5941 gcatctattg ctactccaga cgtattaatt cttccagcaa tgttagggtc aaacttagct
     6001 caaggggctg cttcgatggc tgttgcatta aagagtaaaa ataataatac aaaacaaatt
     6061 gcttttgcag caggtttctc agccttactt gcagggatta ccgaacctgc attatatggt
     6121 gtgactttaa aatataaaaa accgctttat gcagctatga ttggtggtgg attagcggga
     6181 ttatttgcag gtcttactag tgttaaagca tatctatttg ctgtcccatc tttgatagcg
     6241 ttgcctcaat ttatttattc tgatgtgcca tcaaatattg taaatgcttt aattgtggcg
     6301 gtcatttcgg ttgttattac ctttgtatta gcttatatat ttggaatcga tgaagaagag
     6361 agttctagca atttagaagt tgaagctgga gtttcaaata aaaaaatgat attttctcct
     6421 atatcaggag aaatcattcc gttaagcgat gtccaggata aaacattttc agataaacta
     6481 attggagacg gagtagcgat tatcccaagt gaaggtaagg tttatgcacc atttgatggg
     6541 aaaattacaa atatttttcc gactaagcac gcaattggat tgaagagtga tgagggtgtt
     6601 gagttactaa ttcatattgg attagatact gttgagctaa aaggtcaagg ttttattagt
     6661 catgtagaag aaggagacag agttttcaaa aatcagttga tttttgaaat ggacttgaat
     6721 ttaatcaaga ctaaaggcta cgaaacagtt acaccagtaa ttgtaacgaa taccaatgat
     6781 tttctagatg tattagtatt acctaataat cagacaatcg agcattctaa ggaattactg
     6841 gtaatattat aatggaggaa agttatgacc atttttccag atgattttct ttggggtgga
     6901 gctgttgcag ctaatcaagt agagggagca tataatgaag atggtaaggg cttatcagtt
     6961 caggatgtgt tacccaaagg tggattagga gaagcaacag aaaatcctac agaagataac
     7021 ttaaaattga taggtattga tttttatcat aaatataagg aagatatatc cttgttttct
     7081 gaaatgggct ttaatgtttt tcgtacttct attgcatgga gtagaatttt tccaaaagga
     7141 gatgaggaag aacctaacga agctgggttg aaatattatg atgaattgtt tgatgaacta
     7201 catgctcacg ggatagaacc acttgtaact ctttcacact atgagactcc attatattta
     7261 gcaagaaaat atcatggatg ggttgatagg agaatgattc atttttatga aaaatttgct
     7321 cgaacagttt tagaaaggta taaagataaa gttaaatatt ggcttacatt taacgaagta
     7381 aactctgttt tggaattacc atttactagt ggaggaatag atatacctaa ggagaatctt
     7441 tcgaaacaag aattatatca agctatacat catgaattag tcgcctcaag tttggttaca
     7501 aaaattgctc gtgagattaa ttcagagttt aaggtgggat gtatggtatt agctatgcca
     7561 gcttatccaa tgactccaaa tccaaaagat gtatgggcga ctcatgagta tgagaatcta
     7621 aattatctat tttcagatgt gcatgttaga ggttattatc cgaattatgc aaaaagatat
     7681 tttaaggaaa atgacattaa catagagttt gcagctgaag atgcagagtt attaaaaaat
     7741 tataccgtag attttttatc ctttagctat tacatgagtg tgactcaatc tgctcttcca
     7801 acacagtata attcaggaga agggaatatt attggtggtt tagtaaatcc ttatttagag
     7861 tcttcagagt ggggatggca aattgatcca attggactac gtataatttt aaatagatat
     7921 tatgaccgtt atcaaatccc attatttata gtagaaaatg gattaggtgc taaagatcaa
     7981 ctaataaaag atgaacttaa taacttaacc gtccaagatg attatagaat tcagtatatg
     8041 aaagaacatt tattgcaagt tgctgaagct ctacaggatg gtgttgaaat tatgggctat
     8101 acgtcttggg gatgtattga ctgtgtttct atgtccactg cacaactttc taaaaggtac
     8161 ggtctcattt atgttgatcg aaacgatgat ggaagtggta cattaaatcg atataagaaa
     8221 atgtctttta cttggtataa agaagtgatt gaatcaaatg gagaatcctt attcaaatag
     8281 ttttttattg atgactagct attatcacgg ttaaggtggc atttcattaa atatggaata
     8341 aagaagtttg gaagacatta aattctaaac ttctttattt tataggggag tgtcaattaa
     8401 tttaattttg ttacaaaaat tataaatagt taatttagat aaattgttta tataaattta
     8461 gataagaata agctaacaag cagaagtaag ataggtcttg caagaatggt aaaaatctga
     8521 taaactaagt agtaattgaa taggaatctg aaagactagt agcccaaggg aagtatatca
     8581 gggaggagag ccgtgactgc aagctctcta tatgaaagct gggtgaattc acttgcgcat
     8641 ggatttagaa atgaaggtct gacttgtcag atgaaaacgg atggtaccgc gtgtcaacgc
     8701 tccgagtgga gtttttggca tgtggttttc tttttatcta cgagagactg atggaggaaa
     8761 tatgtcaact attgaagaac aattaaaagc gcttcgcgaa gaaacgctgg ctagcttgaa
     8821 gcagattact gctggaaatg aaaaagagat gcaagatttg cgtgtctctg tccttggtaa
     8881 aaagggttcg ctcactgaaa tcctcaaagg gatgaaagat gtttctgctg agatgcgtcc
     8941 aatcatcggg aaacacgtca atgaagctcg tgatgtcttg actgctgctt ttgaagaaac
     9001 agctaagctc ttggaagaaa agaaagtcgc ggctcaactg gctagcgaga gtatagatgt
     9061 gacgcttcca ggtcgtccag ttgcgactgg tcaccgtcac gttttgacac aaaccagtga
     9121 agaaatcgaa gatatcttca tcggtatggg ttatcaagtc gtggatggtt ttgaagtgga
     9181 gcaagactac tataactttg aacgtatgaa ccttccaaaa gaccacccag ctcgtgatat
     9241 gcaggatact ttctatatca ctgaagaaat cttgctccgt acccacacgt ctccagttca
     9301 ggcgcgtgct atggatgccc atgatttttc taaaggtcct ttgaagatga tctcgccagg
     9361 gcgtgtcttc cgtcgcgata cggacgatgc gacccacagt caccaattcc accaaatcga
     9421 aggcttggta gttgggaaaa atatctctat ggctgatctt caaggaacgc ttcagttgat
     9481 tgtccaaaaa atgtttggtg aagagcgtca aattcgtttg cgtccatctt acttcccatt
     9541 cacagagcca tctgttgagg tggatgtttc ttgcttcaag tgtggtggag aaggctgtaa
     9601 tgtatgtaag aaaacaggtt ggatcgaaat tatgggggcc ggtatggttc acccacgtgt
     9661 ccttgaaatg agtggtatcg atgcgactgt atactctggc tttgcctttg gtcttggaca
     9721 agagcgtgta gctatgctcc gttatggaat caacgatatc cgtggattct accaaggaga
     9781 tgtccgcttc tcagaacagt ttaaataatg attagaaaag tagaaatggc agatgttgag
     9841 gtgttggcta aaattgccaa acaaaccttt cgtgaaacct ttgcgtatga taatacggaa
     9901 gagcagttac aggaatactt tgaagaggct tatagtctga aaactttgtc aactgagttg
     9961 ggaaatcctg actctgaaac ctatttcatt atgcatgagg aggagatagc tggttttctc
    10021 aaagtcaact ggggaagtgc tcaaactgag agagaattag aggacgcttt tgaaattcaa
    10081 cgtctctatg tgctacaaaa attccaagga tttggactag gtaagcaact gtttgaattc
    10141 gcacttgaac ttgctacaaa aaatagtttt tcttgggctt ggctaggtgt ttgggagcat
    10201 aatacaaaag ctcaagcgtt ttataaccga tatggttttg aaaaatttag ccaacatcat
    10261 tttatggttg gtcaaaaagt agatacggat tggttactga gaaagaaatt aaggtaagaa
    10321 gaggattctt ctattgaaat gataggaaag gaaaagaact agagtgacaa ctgtcattct
    10381 ggagaactaa attatgcttg tatcttataa atggttaaaa gaattggtgg acattgatgt
    10441 gccatcacaa gagttggctg aaaaaatgtc aactacaggg atcgaggtag agggtgtcga
    10501 atcaccagct gctggtctct caaaaattgt cgtcggtgag gtcttgtctt gcgaagatgt
    10561 gccagagact cacctccatg tttgtcagat taacgttggc gaagaagaag agcgtcagat
    10621 cgtttgtggt gccccaaatg tgcgtgctgg gatcaaggtc atggtggctc ttccaggagc
    10681 tcgtatcgct gataactaca aaatcaaaaa aggaaaaatc cgtggtttgg agtcacttgg
    10741 aatgatctgt tcacttggtg aattgggaat ttctgactca gttgtgccta aggaattcgc
    10801 agatggcatc caaatcttgc ctgaagatgc cgtgccaggt gaggaagtct tttcttacct
    10861 agacttggat gatgaaatca tcgaactttc catcacacca aaccgtgcag atgccctttc
    10921 tatgtgtgga gtggctcacg aagtggcagc catctatgac aaggcagtca actttaaaaa
    10981 atttactcta acagaaacta atgaagctgc ggcagatgcc ctttctgtca gcattgagac
    11041 agacaaggcg ccttactatg cagctcgtat cttggacaat gtgactatcg caccaagtcc
    11101 acaatggttg caaaaccttc tcatgaacga aggcatccgt cccatcaata acgttgtaga
    11161 cgtgacaaac tacatcctgc tctactttgg tcaacctatg catgcttttg acttggacac
    11221 atttgaaggg actgacatcc gtgtgcgtga agcgcgtgat ggtgaaaaat tagtgaccct
    11281 ggacggtgaa gaacgagact tggctgagac agacctcgtg attacagttg ctgacaaacc
    11341 agtagccctt gccggtgtta tgggtggtca ggctacagaa atttctgaaa aatctagtcg
    11401 tgttatcctt gaagctgctg tttttaatgg caaatctatc cgtaagacaa gtggtcgcct
    11461 gaaccttcgt tctgagtcat cttctcgctt tgaaaaagga attaatgtgg caacagttaa
    11521 tgaagccctt gatgcggcag ctagcatgat tgcagagctt gcaggcgcga cggtgcgtaa
    11581 gggtatcgtt tcagcgggtg agcttgatac ctctgatgtg gaagtttctt caacccttgc
    11641 tgatgttaac cgtgtcctcg gaactgagct gtcttatgct gatgtagaag acgtcttccg
    11701 tcgtcttggc tttggtcttt ctggaaatgc agacagcttt acagtcagcg taccacgtcg
    11761 tcgttgggat atcacaatcg aagctgatct ctttgaagaa atcgctcgta tctatggata
    11821 tgaccgcttg ccaaccagcc ttccaaaaga cgatggtaca gctggtgaat tgactgtgat
    11881 acaaaaactc cgccgtcaag ttcgtaccat tgctgaagga gcaggtttga cagaaatcat
    11941 cacctatgct ctgacaactc ctgaaaaagc agttgagttc acagctcaac caagtaacct
    12001 tactgaactc atgtggccaa tgactgtgga tcgttcagtc ctccgtcaaa atatgatttc
    12061 agggatcctt gataccgttg cctacaacgt ggctcgtaag aataaaaact tggcccttta
    12121 tgagattgga aaagtctttg aacaaacagg taatccaaaa gaagaacttc caaatgagat
    12181 caacagcttt gcctttgcct tgacaggctt ggttgctgaa aaagatttcc aaacagcagc
    12241 agttccagtt gatttcttct atgctaaggg aatccttgaa gccctattta ctcgtttggg
    12301 actccaagta acctatacag caacatctga aatcgttagc cttcatccag gtcgtacagc
    12361 cgtgatttca ctcggtgacc aagttcttgg tttccttggc caagtgcatc cagtcactgc
    12421 caaggcttac gatattccag aaacgtatgt agctgagctt aacctttcag ccatcgaagg
    12481 ggcgctccaa cctgctgttc catttgtgga aatcaccaaa ttcccagcag tcagccgtga
    12541 cgttgccctt ctcctcaagg cagaagtgac tcaccaagaa gttgtagatg ctatccaagc
    12601 tgccggcgtg aaacgtttga cagatatcaa actctttgac gtcttctcag gtgaaaaact
    12661 gggacttggt atgaagtcaa tggcttatag cttgaccttc caaaatccag aagacagctt
    12721 aacggacgaa gaagtcgcac gctatatgga aaaaatccaa gcgtctctcg aagaaaaagt
    12781 caatgcagaa gtgcgttaaa cgatagtagg atacaaattc cccctctagt ttatgaacat
    12841 tagaggggtg attttatata aaataattaa aatgtgattt ctgtaaaaat acatctatga
    12901 tcagagtttt gaaaacttac aatgtctaaa tctttaacat agtagtggtt tttaggcaat
    12961 aaaatatgat ctattgttgc attaaaaagt tttggacttt ggctattcca agttcctctt
    13021 tcaaaaggtg gcagtgcatt taatgcgtcc ctatgagagc ttatttttgc aagtgctcca
    13081 tgacgcatag ttgcattaaa atcacctgca ataatagcct ttggatattt tgaagccaat
    13141 tgattatgaa tgatgtttaa gtcttgcttc cagatttcca ttaaacctgg cagaggaggc
    13201 gcagtgtgca aggcaatgat atctggtata ttttgttttc tcgaatgtaa tacaattgtc
    13261 ccgaaccgtg ttgtatgaaa agttttagct tctgtataga aaccataact tttcttgaca
    13321 atcacagtca caggagctat tccactattg gtaggtggag aagtgaaaat atcatagttg
    13381 gccatagaaa gtccaacttg atgaaacaat agtttgattc tctggttttc ttgctcacct
    13441 ctgatattgg tagctagttc agggaatata gccatatcgg cgtcaaaatg gctaaaaatt
    13501 cgctcaatat gttgtgcttc tatttgatta gcgacgttcc aagttactaa ttttaaggtt
    13561 ttagtcttat ttgatgcaga agaaagggtt ttatctgttc cgaataatag taatatgagc
    13621 gatagtaaga gcaaaatgcc agagtaaaac cgtatttgtt tatttttctt gtaaaaagat
    13681 aggaaaaaaa ataagaaact taaaatagca gccatagttg cagtagctaa ggggaaggca
    13741 taaatctgtg ccaagatagg ttgggtagat agccaaatgt taagattaga agatgggaaa
    13801 aaagaataag ttatgatagg gatagaaaag agaatgaccg aaagggcaga gagaaatgtt
    13861 ttaatcattg tgtgagtacc tcaatttgca atggttgctc ggagctaatg tgactttgtg
    13921 actttacttg attgatattc tttttcttgt cttcctcata gattaagtac aacccaagta
    13981 aaaaaagtcc aatttaagat tgaaatgatt aaaaggcaca gaaatgcaag acaattatac
    14041 tacaaaaggc aaacatttga caatcgatag ccgtcgctta atcgaacgat ggaaaaaaga
    14101 aggaaaatca aatagagaaa ttgcctctct acttggaaaa gctcctcaaa ctatccacac
    14161 tgaaatcaag cgtgggacag tccgacaatg tcttggaaaa gggcgcttca aagagattta
    14221 ttctgccgac tacgctcaac agtcttaaga aactaaagga aaagattctc cactatacat
    14281 aaccaaaaac ataaccaaaa attttcgcct gaaatgatgg tgatggctaa aggggttaac
    14341 gtgggaattt caaccattta ctattggatt catcgtggaa aattggggtt aagcaagcag
    14401 gaactgcttt atcctagaaa aggaaaatcg cttaagaaat aggctagcac caactttaaa
    14461 cccactggtc aatctatcga atcataattc gctcattttt ttaaaaatac atagacgtaa
    14521 aaaaaagagg gttaagtgat gaagaatagt gctattggga gtaattggaa ggatgtccat
    14581 ctgagctctt tagcaaggag gaaatccttg aaagtgatat gcgagtggct atcatgagcg
    14641 agttgattga ggctaggtat gaacaaggaa tcagtcagaa aaagctggaa gaagtcagtg
    14701 gaataagcca gcctgtcata gctaggatgg agacaggaaa gactagtcct cagttggata
    14761 cagtcttaaa agtcctagcc agtttaggaa agacactagc aatcgtccca cttgaacagg
    14821 gaaaaagttg actagaatca gttatagtct tttctaataa caggtcatag tcacttataa
    14881 gaattactaa taacatgttt tagtattcta attggaatat agctgtaaag taagaattac
    14941 aaaggatttg agacgtttgc ttcaagtctt tttcttttgt ccgaaacaga tatatagttt
    15001 caaactatat caaaccctaa ttttttacaa ttatacagac gctttctttt ctgttaagat
    15061 agtttcaaca acaaattttg gaggacacat catgtcaact acaatcatcg gtttccctcg
    15121 tttgggcgaa ttccgtgaat taaaatttac aactgaaaaa tactttagaa aagaaatctc
    15181 agaagaagaa cttttagcag cagcaaaaga cttgcgtgct aaacactgga acattgtcaa
    15241 agaaaaaggc atcactgaaa ttccatcaaa tgacttttct cactatgata acttcctaga
    15301 tgcggctttc cttttcaacg tggtgcctgc atccgttcaa aacttggact tgtctgacct
    15361 tgagcgctac ttcgctttgg gacgtggtta ccagggagaa aaaggggacg ttcgcgccct
    15421 tccaatgaag aaatggttca acactaacta ccactacatc gttcctaaat ttgaaaaaga
    15481 cactcaagta aaattggctg gtcacaagat tttcgatgag ttccaagaag ccaaagaact
    15541 tggattgaac actcgtcctg ttcttgtagg tcccttcact ttccttcaat tgtcagactt
    15601 tgaagaaggc gtgaaagcag acgacttcgt agatagctta gtggctgctt accaagaagt
    15661 ttttgctaaa ttggctgaac ttggtgcgac tcgcatccaa ttggatgaag cggctcttgt
    15721 aaaagacttg acagctgaag aaaaagctct cttcttgaac ctttacaaca aactcttggc
    15781 tgacaaaaaa ggtcttgaag tcttgcttca aacttatttc ggtgatgttc gtgacgtata
    15841 cgctgacctt gtaaacttac cagtagatgc tatcggtctt gactttgttg aaggtaagaa
    15901 aactcttgaa ctcgttaaag gtggcttccc agctgacaag actctctatg taggtattgt
    15961 caatggtaaa aacatctggc gcaacaacta cgaaaagagc ttggctgttc ttgagcaaat
    16021 tccagctgaa aacatcgttt tgacaagctc ttgctcactt cttcatgtgc catttacaac
    16081 tgctaatgaa gaatttgaac cagcactctt gaaccacttt gcctttgcag ttgaaaaatt
    16141 ggatgaaatc cgtgacttgg atgctatccg aaatggtcaa ggttcagaag ctcttgcagc
    16201 caacaaagaa ctctttgcga ctgagcgtgt tggtgaaaat gctgaacttc gtgcgcgtat
    16261 cgctggcttg acagacgcag actacactcg tttgccagcc tttgcagaac gtgaagctat
    16321 ccaagaagaa gctttcaaac ttccagctct tccaacaaca acgattggtt cattccctca
    16381 aacaaaagaa gttcgtgcca aacgtttggc ttaccgtaaa ggtgaattgt ctcaaaaaga
    16441 gtacgacgct ttccttgctg aaacgattga cgaatggatc aagtggcaag aagatattga
    16501 ctttgatgtc cttgttcacg gtgaatttga gcgtaatgac atggttgagt acttcggtca
    16561 aaacttgtca ggatacctct tctctaaaaa tggatgggta caatcatacg gtatgcgtgg
    16621 ggtaaaacca ccaatcatct ggggtgatgt cactcgtctt aaccctatca ctgttaaatg
    16681 gtctagctat gcacaaagtc gtacaaacaa acctgttaaa ggtatgttga ctggacctgt
    16741 taccatcctt aactggtcat tcccacgtga agacatctct atcaaggatt caactctcca
    16801 aatcgccctt gctatcaagg atgaagtact tgaccttgaa gctgctggtg tgaaaatcat
    16861 ccaaatcgac gaggctgctc ttcgtgaaaa attgccactc cgccgtagcg actggtacga
    16921 agactacctt gactgggcta ttcctgcctt ccgcttggta cactcaacag tagcgccaga
    16981 cacacaaatc cacactcaca tgtgttactc agaatttaca gatatcatcc cagctatcga
    17041 taacatggat gcagacgtta tttcctttga agctaaccgt tcaaaccttg aaatcttgga
    17101 cgaactcaaa gcgaaaaact tccaaacaga agtgggacct ggggtttacg atatccactc
    17161 acctcgtgta ccaaatgaag gcgaaatcga caacacaatc gaagccatcc ttgctaaagt
    17221 gccaagcaag aaagtttgga tcaaccctga ctgtggtttg aaaacacgtg gtatcccaga
    17281 aacaaaagaa agcttgatcc gccttgttga agcagctaaa gctgcgcgtg agaaattgta
    17341 agattggatt tctctccata cagtggttgt tcaaaaagaa atcaactaga aaaggttatt
    17401 acatatgtca cgccaaacac cgtcactctc atttgaagtg ttccctccaa acccagccgt
    17461 gggtaatgat aacattattt cagccttgca ggatatgcag gagttggctc cccattttat
    17521 cagtgtaact gccagcaata ataaatttaa tatcaaggaa acgacggttc gtttggctga
    17581 ctttatccaa aatgatttgg cgattccgac cattgcccac ttgccagcta tttatctaac
    17641 taaggacaag gttgctgaaa ctattgctga cttggacaaa gttggggtgc agaaaatctt
    17701 ggctcttcgt ggggatatta ttccagacgt ggaaccacaa aaggatttcc gctatgcaac
    17761 cgacttgatt gagtttatca aggaacaaac ccctcacttt gatattattg gagcttgcta
    17821 tccagaaggg catccagatt cgccaaatca gatttcagat attcaaaatc ttaagaagaa
    17881 agtggatgca ggctgttcga gcctcgtaac tcagctcttc tttgacaatg agcgcttcta
    17941 tgattttcag gacaagtgta tcttggctgg gattgatgtt cccattcatg caggaattat
    18001 gccaattctg aatcgaaatc aggctctccg actcttgaag acttgtgaga atatccatct
    18061 tccacgcaaa tttaaagcca tcttagacaa gtatgagcat gaccctgagt cgctcagagc
    18121 agcaggactt gcctatgcag tggaccaaat cgtggacttg gtaactcagg atgttgccgg
    18181 tgtgcatctc tatacgatga ataatgctga tacagcaaaa tacatccatc aagcaaccca
    18241 tgccttgttt aatcaccagt ctctaggata ataaaagcaa accattcttc tcaggtgagg
    18301 ggaatggttc ctttttaatg gtaaagacct cactttttaa gaaaaatatg ataaaataaa
    18361 ttctgtacgt acttgataca aagatgaagg tattaaataa tacaatgcat ttaaactgta
    18421 agatttaacg acacgagtga gtcaaatcga tgttatttga cgagagagtt agtaaagcat
    18481 ttagctaatc gcccgatagc gattagcgtg aaagctttag atactttagt attaaacctt
    18541 tctaatgatg tatcttgaaa actgaacttg cctacaactc tgtggaaaaa gataaatctc
    18601 cctaggagta gtcgctccgt cgttcgattt cctatcttcc tttgagttgc ttgacggctt
    18661 ggtatcttga tgacttgaat tttggtctca tttcttagga aaaaagataa gcttcctagc
    18721 actcgtcgag tgcgacgtca ccttcctatt tttctacaga aatgttcggc aagccgaacc
    18781 gtccaaaata tcttgtgtaa ttgaacacgg ccgaaaagct gtgtaaaaaa gataaactgt
    18841 cttgtcttca tcgaagactt cgtcagtttc ctatttttac tttgcttttg acgtccttgg
    18901 tatcttgatc tttgtaggca aggcgtataa tttcatcaat ccaaagggga ttaaaatggc
    18961 aaaacaagtg tttcaaacga cttttgcggg tcgtgagtta attgtagaga ctggtcaggt
    19021 tgctaagcaa gcaaatggat ctgttgtcgt acgttacggt gagtcaactg tcttgactgc
    19081 tgccgttatg tctaagaaaa tggcaactgg ggatttcttc ccactccaag tcaactacga
    19141 agaaaaaatg tatgcggctg ggaagtttcc tggtggcttt atgaaacgtg aaggacgtcc
    19201 ttcaacagat gcgaccttga cagcgcgttt gattgaccgt ccgattcgtc ctatgtttgc
    19261 ggaaggtttc cgtaatgaag tccaagtcat caatacagtg ctttcttatg atgaaaatgc
    19321 atctgcacca atggctgcta tgtttggttc atctttggca ctgtctattt cagatattcc
    19381 atttgacgga ccaattgctg gggtacaagt gggatatgta gatggccaaa tcatcatcaa
    19441 cccaagtcaa gaacaagcag agcaatctct tcttgaattg acagtagctg gaaccaagca
    19501 cgctatcaac atggtagagt ctggtgccaa agaattgtca gaagaaatca tgttggaagc
    19561 gctccttaaa gggcacgaag ctgtcaaaga attgattgcc ttccaagaag aaatcgttgc
    19621 tgctgtcggt aaagaaaaag cagaagtgga attgcttcac gtggatgctg aattgcaagc
    19681 tgaaatcatt gcagcctaca acagtgacct ccaaaaggca gttcaagtag aagagaaatt
    19741 ggcccgtgaa gctgcgactc aagtagtgaa agaccaagtg actgccgttt acgaagaaaa
    19801 atatgcggac cacgaagaat ttgaccgtat tatgcgtgat gtggctgaaa tcttggaaca
    19861 aatggaacac gcagaagtgc gacgtttaat tacagaagac aaggtgcgtc ctgatggtcg
    19921 taaggtcgat gaaatccgtc ctttggatgc ggttgttgac ttccttcctc gtgtacatgg
    19981 ttcaggtctc tttactcgtg ggcaaactca agctctttca gtcttgacct tggctccgat
    20041 gggagaaact caaatcattg atggtttgga tccagagtac aagaaacgct ttatgcacca
    20101 ctataacttc cctcaatatt ctgtagggga aacaggtcgt tacggtgcgc caggtcgtcg
    20161 tgaaatcggt cacggtgccc ttggtgagcg tgctcttgct caagtcttgc caagcttgga
    20221 agaattcccc tacgctatcc gtctagtagc agaagttttg gaatcaaacg gttcttcatc
    20281 tcaagcttct atctgtgcgg gaactcttgc ccttatggct ggtggtgtgc caatcaaggc
    20341 gccagtagct ggtattgcta tggggcttat ctcagatgga aataactaca cagtattgac
    20401 agatatccaa ggtttggaag atcactttgg agatatggac ttcaaggttg caggtactcg
    20461 tgatgggatt acagcccttc aaatggatat caagattcaa gggattactg cagaaatctt
    20521 gacggaggct cttgctcaag ccaagaaagc gcgttttgaa atccttgatg tcattgaagc
    20581 aaccattcca gaagttcgtc cagaattggc tccaactgct ccgaaaattg atacgatcaa
    20641 gattgatgtg gacaagatta agattgtcat cggtaagggt ggagaaacca tcgacaagat
    20701 tatcgctgaa acaggtgtta agattgatat agacgaagaa ggaaatgtgt ctatctactc
    20761 tagtgaccaa gatgctatta accgtgccaa agaaattatt gctggtttgg ttcgtgaagc
    20821 caaagtggat gaagtttacc gtgctaaagt cgttcgtatc gagaaatttg gtgcctttgt
    20881 taacctcttt gataagacag atgcccttgt tcatatctct gagatggctt ggactcgtac
    20941 caatcgtgta gaggatttgg tagaaatcgg ggatgaagtt gatgttaagg ttatcaaaat
    21001 tgatgaaaaa ggccgtatcg atgcctctat gaaggctctt ctacctcgtc cgccaaaacc
    21061 tgagcatgat gaaaaaggtg aaaagtctga gcgccctcac cgcccacgtc atcaaaagga
    21121 ttacaaacct aagaaagaat ttacagaaac atcaaaagat tcagaataag aaaaggagaa
    21181 atgtatgggg tggtggcgcg aaaccattga tattgtaaaa gaaaatgatc cagcggcccg
    21241 caccactttg gaggttttgc tgacttatcc aggtgtcaag gccttggcgg cccaccgtct
    21301 ctcgcatttt ctctggaagc acggcttcaa actattagct cgtatgtaca gtcagttttg
    21361 gcgcttttgg actcagattg agattcatcc aggagcccag attgattcag gtgtttttat
    21421 tgaccatggt tctggtctgg tgattggaga gacagcgatt gttgaaaaag gcgttcttct
    21481 ctatcacgga gtgactctcg ggggaacagg gaaagactgt ggcaaacgcc atccgactgt
    21541 acgaaaggga gccctcatat cagcccatgc ccaagttatc gggcctgtgg aaattggtga
    21601 aaatgccaag gtcggtgctg cagcagttgt cgtagcagat gtacctagtg acgtgacggt
    21661 tgtcggtatt ccggccaaga ttgtccgtct tcatggtaag aaagatgagc ctgtcattca
    21721 cgaagtcgaa gaaaaacgag agtattatgt caataaactc gagcaggcta aagatgccag
    21781 tcacagatcg tctggtttgt agaggatacc tatatgattc aagcaagaaa caagttaagc
    21841 caagaggagc tatctgaggc gaaaaaagta attaactgtt gccaaaacta tgacggtacc
    21901 tatcgcgatc cctatctctc taacatgctt aattttgacc caaacatgcc cgcctttttc
    21961 ctttattatg aaaaaggcga acttgttggt ttattaactg tctatgcaga tgaccaagat
    22021 gtagaagtga cgatactggt tcatccaggt catcgccgtc aggggattgc gcgtgcattg
    22081 tttactagtt ttgagagaga aacagcttct ttccccattc ggtcagtcac ttttcagaca
    22141 gaacgtattt ttttagagaa tcatcctgac tttgccagta actggggact aatcgaggat
    22201 gaagagacag aaacttggtt aggtaaggat agaagacctt atccgttagc aaatgtttcc
    22261 catcttgaag ttttgttagc agatagttcg tatcaggatc aaattagtca attaaaattt
    22321 caggcatttt cagaggaaca tgaatcgaga gaagttgtgg atagatatgt cgctgaagct
    22381 ctgaaagatc cagaaagccg actatatatt ttattaaaag ctggtcaggt tattggaact
    22441 tgtacggttg atttatcgac taatacgaat tacctctacg gtttagcaat attggaacct
    22501 gaacgtggaa aaggctatgg aagctactta gcaaaatccc tcgtcaacca actaattgag
    22561 caaaatgaca aggaatttca gattgcagtg gaagatagca atgtaggtgc caaacgtttg
    22621 tatgaaaaaa ttggctttgt caaacagact caggtggttt atctgaatga gaaaggagca
    22681 agggattccg aagtgtagag atattcggac tgaaatttac ttgaactttt agtgatgaaa
    22741 ctaattgttc ttggatttca gctttcctga ttatgattta tgattaaaat ctatgacacc
    22801 atgtctcgtg atttgcgaga atttgtcccg attgaggacg gcaagatcaa gatgtatgtt
    22861 tgtgggccaa cggtgtacaa ctatatccac gtaggaaacg cccgttcgac ggtagctttt
    22921 gatacgattc gtcgctattt tgagtaccgt gggtataagg ttgcctatat ttccaatttt
    22981 acagatgtgg atgataagat tatcaaccgt gccagggaag aaggtatcac gcctcaggag
    23041 gttgcggata agtacatcgc tgcctttcgt gaggatgtga cggcattggg cgtgaaacct
    23101 gcgactcgcc atccgcgtgt agtggagttt atggcagaca tcatccgttt tgtggaagac
    23161 ttgatcgaga aaggctttgc ctatgagagt caaggggatg tctatttccg tgtagaaaaa
    23221 tcccacaact atgctaaatt ggctaataaa accttggaag atttggagct aggtgcttca
    23281 ggtcgtaccg atgaagaaac ggctcgtaag gaaaatcctg tagactttgc tctatggaaa
    23341 tcttccaaac caggtgagat ttcttgggat agtccttggg gacctggacg tccgggttgg
    23401 catattgagt gttcagtcat gtcgacagag attttaggtg ataccattga tatccacggt
    23461 ggtggagctg acctagagtt tccacaccac accaatgaaa ttgcccagtc tgaagctaaa
    23521 acaggcaagg cttttgctaa ctactggatg cacaatggct ttgtcaatat cgacaatgtc
    23581 aaaatgtcta agtccttggg taacttcatt acggtacacg atgcccttaa aactcttgat
    23641 gggcaagtac ttcgtttctt ctttgcgacc cagcactacc gtaagcctat caacttcacg
    23701 gaaaaggcag ttcgcgatgc cgagaccaat ctcaagtatc tcaagaatac ttacgaacaa
    23761 ccatttacag gaaatgtgga tgcccaagag ttacaaaact ttaaagataa gtttgtagca
    23821 gctatggatg aagattttaa tgctgccaac ggtatcacag ttgtctttga aatggccaaa
    23881 tggatcaact cagggaacta tgatgcaagt gtcaagcaag ctcttgcaga tatgttagaa
    23941 atttttggaa ttgtctttgt tgaggaagtt ttggatgcag agattgaaga cttgattcaa
    24001 aaacgccaag aggcgcgtgc caatcgtgac tttgcgacag cagaccaaat ccgtgaccaa
    24061 ttggttactc aaggaattaa gctccttgat accaaggatg gagtgaggtg gacacgtgat
    24121 tgatgtcaat ctcattaacg ggattgcgct agcctttgag ggggatgcgg tgtattctat
    24181 gtatattcgc cgtcacctca tcctcaaagg tatgaccaaa cccaataaac tccatcaaga
    24241 agcaactaag tacgtgtcag ccaaggctca ggctcgcctg attgctctca tgttggagga
    24301 gcaggtccta acggaaaaag aagaagaaat ctacaaacgt ggccgcaata ccaatagcca
    24361 cacaaaggct aaaaatgcag atgtcgtgac ttatcgtatg tccacgggat ttgaagcggt
    24421 tatgggctat ctccatatga ctgagaatct ggaacgtctt gagagtttgg tttcatggtg
    24481 catccaaaaa gtggagggct agaacatgag ggcaaaagaa ctacaagact ggtttcctga
    24541 ggctcggatt tcagaccaac cagtagagaa agagggctat ctcacgctcc ctttagcttc
    24601 tcagcagtgg attttgctgg aggaagctgg gctcagcgag cgtgaaaagc agttggttgc
    24661 ccttttgacc cagcaggagc aggctcgttc gctaaaccct tggtattcct atctggttga
    24721 gggcaaggga caggcaccgc aagtttttaa aaagattcag ttggtttatt gccatctttc
    24781 ttattttcag caggaaaatc tggcttcttg gctagatatg atgcggactc tttttccgaa
    24841 ttgtcagaca gtgctacagg tcggagctca ggattatgtt ttcgtgcttc aacaagacaa
    24901 atacacttct gtaagagata ttttaagtga tacgattgaa gcggttgagt atgactttgg
    24961 acttcgtctt tctatcatgt tgggtcaggt ttggtctcag acgggacatc aagccctatc
    25021 agacttaatc aaagctgagc gggatttgtt caagacatgg tggcgtcagg gtcaccaagg
    25081 tgttcatact ttttctcagc tctatctttg gagtatggga gaaagactcg tggacttgaa
    25141 gccaatcaag gaatgtctac accagatgat tttggatcaa gatcagattc aggaaatcat
    25201 tctctctctt tgggaaaata gtgctgttct cactaaaaca gcccagcaac tctatctgca
    25261 ccgcaattct ctccaataca agattgataa atgggaagag ttgacagggc ttcagttgaa
    25321 agagttgacc gacctgacct tgtgttatca attgatttta ggttctttgt caactatagt
    25381 tggtttgtaa agaagttaat atttggagaa gaggattgcc atcttctcca tttttatgtg
    25441 cagagttata gtggcttgat gctgggaaag tacactgtga ctgctaaaac attcctagaa
    25501 gctgctttga tttccctaat ctatttatgc aaattttatg ttattttact ataacagttg
    25561 ttgctaagcc aaataatagt ggggaagttc atttagacgt aagcattgaa gataatcagg
    25621 gaggtagtgg gtataatttc agttctgttt caagtagctc acaaacagct aaatatgaag
    25681 gaactgttta taataacaat tcatcattat atataacgat tgataaaacg tctgatgcaa
    25741 cagctctttt gaaattaaag ttgaataatg ttgataatca acctgctact gaagttccta
    25801 gttcaggaat tactgtaaaa ttaaatgcta aagataatgc tggaaactgg acaagtgctt
    25861 cgaataaaaa agaagtaaca gtaaaaattg tttctgctaa accgacatat ccagacaaaa
    25921 tcttagtgaa aaatcctgat aatataaaag atacagaaaa aatgccatta ttgaaaaatt
    25981 gaaagaggca aataaaaatc atccagcagg agctccaacc tttgctaaag gtgaaggaga
    26041 gcatgcaaat gatattgtag caacttattc agatggtaca acttattatg taccgttaaa
    26101 tgatgtgaca aaatatgcga ggtagtggct gtaccactca cttattcacc tccccgtgat
    26161 ttgtagtagt gataggtttt ctcactatta ttataaaaca aaataaagat cacaacactt
    26221 tttcattctg tgttgtgcct tgagtgaaac gaaaggaatg aattataaat atgaaaagta
    26281 tagtcactag catagatgag cgcttgcgta ctcgcctacg agtgattatc tggaagcaat
    26341 ggaagaagaa atcgagacga ttatggggat tgcttaagtt aggggttcct aaatggatag
    26401 cagataaggt atctggctgg ggcgaccact atcaattata gtaaaatgaa ataagaatag
    26461 gacgaattgt tcaggacagt caaatcgatt tctaacaata ttttagaagt aga
//
LOCUS       DAVKNL010000026        25420 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVKNL010000026 DAVKNL010000000
VERSION     DAVKNL010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25420
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.026"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            56..805
                     /locus_tag="VW411_001097"
     CDS             56..805
                     /locus_tag="VW411_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU9793835.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVAHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMYQEELDEKNYIDRISSILCYKSQILI
                     IWKSVEKLLNNIKELYLRNGAAYSIRFWIKK"
     gene            818..1989
                     /locus_tag="VW411_001098"
                     /pseudo
     CDS             818..1989
                     /locus_tag="VW411_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
     gene            2385..3248
                     /locus_tag="VW411_001099"
     CDS             2385..3248
                     /locus_tag="VW411_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU9793836.1"
                     /translation="MIERMELGEFYKELRLARKLKQSDVACEGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMRIGRKVVNRFAHQDIAALEQLLEEV
                     DQEQMAQTYRRLNAIVIKDAIHSLNKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNILSELMERKLFSYIPIFE
                     AELERMLRPYDVFEKVSWQFLKKMSVFLQTKGSNQKEIERFIQSLQVLENPQLIALFE
                     LRFQQYKELID"
     gene            3534..3767
                     /locus_tag="VW411_001100"
     CDS             3534..3767
                     /locus_tag="VW411_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793837.1"
                     /translation="MLNLQFAETMELTEAELQDVRGGNLVNSMGGGGRSGISGWGVPG
                     IYPGWGNQGMSPNRGAFDWTIDLADGLFGRRRR"
     gene            3789..4466
                     /locus_tag="VW411_001101"
     CDS             3789..4466
                     /locus_tag="VW411_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9793838.1"
                     /translation="MKKMKEVKFHLATGLLILTYYLIFNVTSDLDFMVALSDNMYYVF
                     QVLLVLILGTIATIAFVKSEHWKECGRFQFRWSYLGVFLLSFFLLFVWANLTTYIFPR
                     TQNGSTVVEVATNLTGISYFVTRILYTSIIAPVSEEVVCRGLLMTSLSKVKRYYLDVL
                     VSAAIFGAMHVLQYGWITTDFIKYFGMGLIFCMMFRYTRSIYWAIALHASWNSFLLIV
                     TLLVFGY"
     gene            4478..5143
                     /locus_tag="VW411_001102"
     CDS             4478..5143
                     /locus_tag="VW411_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9793839.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVTGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVVWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEDMIYRGLVMTALEKGKKWGLDVL
                     GSAVLFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            5215..6441
                     /locus_tag="VW411_001103"
     CDS             5215..6441
                     /locus_tag="VW411_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9793840.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFAKFLLYSIVSLSLSGQSFSLL
                     LVAFICLINLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPISTLFLRNQPFLNLQTGQSLALLATLEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(6838..6915)
                     /locus_tag="VW411_001104"
                     /pseudo
     CDS             complement(6838..6915)
                     /locus_tag="VW411_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            6986..7642
                     /locus_tag="VW411_001105"
     CDS             6986..7642
                     /locus_tag="VW411_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEU9793841.1"
                     /translation="MPTALGYVSIGLACGIIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSFFLLLTVMSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            7632..7841
                     /locus_tag="VW411_001106"
     CDS             7632..7841
                     /locus_tag="VW411_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEU9793842.1"
                     /translation="MVSKYLLLAVIFSGLVTWIPRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIK"
     gene            8059..8889
                     /locus_tag="VW411_001107"
     CDS             8059..8889
                     /locus_tag="VW411_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9793843.1"
                     /translation="MKKIVKYSSLAALALVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPKPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            9043..9897
                     /locus_tag="VW411_001108"
     CDS             9043..9897
                     /locus_tag="VW411_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU9793844.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKSLFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            9997..11370
                     /locus_tag="VW411_001109"
     CDS             9997..11370
                     /locus_tag="VW411_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU9793845.1"
                     /translation="MVFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFDPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            11363..12424
                     /locus_tag="VW411_001110"
     CDS             11363..12424
                     /locus_tag="VW411_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEU9793846.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSELSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGVQ"
     gene            12426..13118
                     /locus_tag="VW411_001111"
     CDS             12426..13118
                     /locus_tag="VW411_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9793847.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(13148..13717)
                     /locus_tag="VW411_001112"
     CDS             complement(13148..13717)
                     /locus_tag="VW411_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU9793848.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADGVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            13852..14466
                     /locus_tag="VW411_001113"
     CDS             13852..14466
                     /locus_tag="VW411_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU9793849.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            14468..16114
                     /locus_tag="VW411_001114"
     CDS             14468..16114
                     /locus_tag="VW411_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9793850.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHMGETVDK
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            16126..17412
                     /locus_tag="VW411_001115"
     CDS             16126..17412
                     /locus_tag="VW411_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9793851.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHSDEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISGHPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVSITKQLFIQFVMDVF
                     HLFEHLKADDMTDIVKTIHAIQSFDELVSYIKETLISFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            17464..17997
                     /locus_tag="VW411_001116"
     CDS             17464..17997
                     /locus_tag="VW411_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9793852.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELT"
     gene            18075..18545
                     /gene="nrdI"
                     /locus_tag="VW411_001117"
     CDS             18075..18545
                     /gene="nrdI"
                     /locus_tag="VW411_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEU9793853.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(18838..20100)
                     /locus_tag="VW411_001118"
     CDS             complement(18838..20100)
                     /locus_tag="VW411_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEU9793854.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
     gene            complement(20410..20868)
                     /locus_tag="VW411_001119"
     CDS             complement(20410..20868)
                     /locus_tag="VW411_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEU9793855.1"
                     /translation="MKKQVFHDAATGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWATIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(20865..21305)
                     /locus_tag="VW411_001120"
     CDS             complement(20865..21305)
                     /locus_tag="VW411_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9793856.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            21738..22604
                     /locus_tag="VW411_001121"
     CDS             21738..22604
                     /locus_tag="VW411_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9793857.1"
                     /translation="MGTLLATRLKNRRKELKMSQRELAEGICKQGQISRLESGEFTPG
                     ADFLNALAKKLKVSMDYFFDEQVVEKVEELSEFKKLAQTFITNRNYESLKYIYELENV
                     KAHRLSLADKIYMEWIKSLIDFYFYGQKEDAIVRLEGVLSQLSVSDLNYLQVSNTLFN
                     FYYDIGDLGRFNKIREKLEYQVNHLNLSTIEELELSIKFNYNVCRYLWLQKNIEEAIT
                     KITATIKQCKAYRTNYLLADLYLLMGNVSENFSSKSSVKEYFETAYFLYKLEENMSMT
                     LKVEHYIADITE"
     gene            22941..23498
                     /locus_tag="VW411_001122"
     CDS             22941..23498
                     /locus_tag="VW411_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase family
                     flavoprotein"
                     /protein_id="HEU9793858.1"
                     /translation="MDKKNLLVLVTGSVGASNVDTYLYYIKKFYNVKVILSENSKKFI
                     SKELISYFCDKVYDEIFVEEVVPHVFLPYESDLLLILPATANVIGKIANGIADDLVTA
                     TVLNFNKKIIFCPNMNSTMWDNHIVQRNVSILKELGHIFLFESKKTYEVGLRKAIDST
                     CSMLQPQSLVKELIKLENIVLEEGH"
     gene            23719..24825
                     /locus_tag="VW411_001123"
     CDS             23719..24825
                     /locus_tag="VW411_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEU9793859.1"
                     /translation="MINKKIQQVVLESLQNFLNGNFISPCVVYDFGLLETVLDEFKNQ
                     IPVTFNYQLFYAVKANSNEKILEFLVDKIDGVDVASLSELDVAKKFFPPTQISVNGPA
                     FSYETLYNLIKKQYKVDINFLEHLQQFSPKESVGIRVTEPDELNNRMSRFGINICSDN
                     WTSNLQNPLITRLHFHFGEKDDKFIVKLDKILFKLQEINKLREVREINLGGGFMKLFM
                     ENRLKEFFLSLMEIYKKYDIDSTVTTIIEPGSAITSFSAYMITSPVNVSEVNEQQVIT
                     LDTSIYTNTLWFVPHIITTLNSSSKERYSTILYGNTCYEHDKYKMKVSLPRLTQNSSI
                     VFFPVGAYIKSNHSNLHRNDFMREVYLWTKNLTY"
     gene            24801..25420
                     /locus_tag="VW411_001124"
                     /pseudo
     CDS             24801..25420
                     /locus_tag="VW411_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358548.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         7797 a         4265 c         5383 g         7975 t
ORIGIN      
        1 tggaatttaa tcagacactt agtgagtttt tagataaatt gtagagggaa tggttatgag
       61 taacaatact aagtatatct ttttatctcc acatttagat gacgcaattt tttcctgtgg
      121 tgattatatt tcaaaattaa cttcagaagg agaaattgta cttgttatta ctatattttc
      181 tggttaccct ttgagccaac agctacagcc ctctgctaag caatttcata agttgtgtaa
      241 ccttgggaaa tatccgattg aagaaaggaa gaaagaagac aggttagctt gtgaacgtct
      301 acaatgtgat tttagacact tatcgtacta tgaatgtctc tataggaaag atagaaatgg
      361 caattttcta taccgtcata tctatagtga attaaaaaat gaggacacat taaagaatga
      421 tatcataaaa gagctgttaa tgcatttaga cgacaaatgt gtcgtatatt gtccgctttc
      481 acttggtgac catgttgctc atgtttttgt gaatagtatt ggaagggcgc ttgaatttat
      541 gcgttataaa gtaatatatt atgaggactt cccatatgta agtgatagta gtatggttag
      601 ttatatggga aaaactaaag aattaaaaat gtatcaagag gaacttgatg aaaaaaatta
      661 tattgataga atatcatcaa tattgtgcta taaatctcag attttgataa tctggaaatc
      721 agttgaaaaa cttttaaaca atattaaaga gttgtattta aggaatgggg cagcttattc
      781 tattagattt tggattaaga aataaggaag tagttttatg aatatagcag taattggttt
      841 ggggcatgtt gggctggcct acgcgttact atttgcatct aaatataaag ttgttgcata
      901 tgatatagat tctgtaaaaa taaataattt aaaaaagggc attcttccat ctaaaaatga
      961 agagcttatg aagttttttt gcgagaataa cttaaatatt actttttttg atacattttc
     1021 tgaaattaaa aataatattg attattatat tattgcgctt ccgacagatt atgatgagaa
     1081 aattggtagt tttaatacat atgaaatcga acaaacggta tcgaagattc tgagggtaaa
     1141 acctaatgga aagattattt taaagtcaac agttccgatc ggcttttcaa acaaattaaa
     1201 aaggctgttt gatacaaaaa atatcatttt tgtccctgaa tttttgagag aaggttgttc
     1261 tatatatgat aatttatatc caagtcgcat agttgttgga gatgagacag ttgaaggaag
     1321 aaaaattgca gagttgttcc tttcgattag tactcatagt actgccaata ttaaaaatgt
     1381 tatgttagtt tctcctactg aagcagaagc aattaagctt ttttctaaca cattcttagc
     1441 tctccgtgtt gcttttttaa tgaactagat tcttttgctg agaggagaag cttaaatgct
     1501 gaagttgtaa taaaaggtgt ttgtttagat ccaagaattg gaaattttta taataatcct
     1561 tcttttggat ttggaggata ttgtcttccc aaagatacta aacaattaaa aaaagaattt
     1621 atagaaataa atgccccagt gatagaagcg attgatattt caaacacaaa tagaaaacag
     1681 ttcatagtta aacaaatatt ggaacgaaag ccaaagatag taggaatata taaattgggg
     1741 atgaaatata attcagataa ttacaaagag tcagctattt taagtataat taatgaacta
     1801 ttgattgtcg gtataaaaat tttagtatat gaaccaaatt taaacgttag tatagataac
     1861 gtaattattg aaaagaattt tgaactattt acaaaacaaa gtgatctaat tgttgcaaat
     1921 agatgggata ggggccttga agcttataaa gataaagttt acacaagggg gatctggata
     1981 agagattaac tattgatatt aaaaaggcaa taatttataa aatttgtaca aaaaaatgtt
     2041 aaattttcac attttcatag tatactgttt aatagaaagt aggttctttg taaccgctcc
     2101 attataaaat cctttcactc ttccggtata ctattaggaa aaacataact ggaggatatt
     2161 ttgagctcac acctcatcgt tccagtggct gttccccaag ctcgtgtact gcccaaaaaa
     2221 gttagacatt ctatttagaa cgaggattga gttctgtatt gtacaaggct cggtcctttt
     2281 agagtcagct taaggctggc ttttttattt tttctaacta tcagaatatt ttgacaaatg
     2341 attgtaaata atgatataat atgcaaaagc taggaggtgg tcagatgatt gagagaatgg
     2401 aactggggga attttacaag gaattacgtt tggcgagaaa gctcaagcag tcagatgtgg
     2461 cttgtgaggg tctaacagct tctcagttgt ccaagtttga actagggcag tctatgctgt
     2521 ctgcggataa gttgattcta gctatccaag gtataaatgt gacctttgat gagtttgggc
     2581 acaagctcaa caactatcaa gaatctccac atatgcgaat cggtcgaaag gttgtgaatc
     2641 gctttgccca tcaggatata gctgctttag agcagctatt ggaggaagtc gatcaagaac
     2701 agatggcgca gacctatcgt cgtttgaatg ccatcgtgat taaagatgcc atccattcac
     2761 taaataaaag ctacccacta gcagaggagg atagcgagtt tttgaccacc tatctctatg
     2821 ctatcgagtc ttggacctgg tttgaactct atctcttttg caataccatg cccttcttga
     2881 gcaatcaaga tctgattttt ttatcaacct ccttactcga aaaatccaaa gaatttaaag
     2941 agttagtaca caatcgattg tatatgaagc aaggactctt aaatatctta tcagaactca
     3001 tggagcgaaa acttttctcc tacatcccaa tcttcgaagc cgagttggag aggatgttac
     3061 gaccgtatga tgtttttgaa aaagtatcgt ggcaattttt aaagaagatg agtgtctttc
     3121 ttcaaacaaa gggaagcaat caaaaagaga ttgaacgctt tatccaatct ctgcaggtat
     3181 tagaaaatcc acaattaata gccctttttg aattgcgttt tcagcaatat aaagaactta
     3241 tagattagtt agaaatggca aaataataat aatctcagca acgattttca ataatgttaa
     3301 aatttgtttc ttcatatttc tccactcctc attagtagta actttattat accagagaag
     3361 taatcaaatt caccaaaatc gcaaatctga aatttctata agaaaaaata tcaaatatgc
     3421 gattttctaa aataagccaa tgttcgtgtt atactgtact tgtaaagcac ttgaagcaaa
     3481 tcctaggtcg cagaagtggt ttacaaatga agataattga aggagtgtaa aagatgttaa
     3541 atttacaatt tgcagaaaca atggaattga cagaagctga gttgcaagat gttagaggag
     3601 gcaacctcgt aaattctatg ggaggtggtg gaagaagtgg tatatctggt tggggcgtcc
     3661 caggaattta ccctggttgg ggcaatcaag ggatgagtcc taatcgtgga gcttttgatt
     3721 ggactataga tttagcagat ggattgtttg gccgccgtag aagataggct cttataggag
     3781 ataataggat gaagaaaatg aaagaagtga aatttcacct agcaactggc ttgttgattt
     3841 tgacttatta cctcattttt aatgtcacat cagacttaga ttttatggtt gctttgtctg
     3901 ataatatgta ctatgtgttc caagttttat tggtacttat tctgggaact atagctacaa
     3961 ttgcttttgt caaaagtgaa cattggaaag agtgtggtcg tttccagttt cgttggtctt
     4021 acctgggtgt tttccttctt tcttttttct tattgttcgt gtgggcaaat ctaacaacct
     4081 atatctttcc tcgtacccag aatggaagta cagtcgtaga agtagccacc aatttaacag
     4141 gaattagcta ttttgttacg cgaatacttt atactagtat tatcgcacct gtatcagaag
     4201 aggttgtttg tcgtggtctt ttgatgacaa gtttatctaa agttaagaga tactatcttg
     4261 atgttcttgt ttccgctgcc atatttggcg ctatgcatgt tttgcagtat ggttggataa
     4321 cgacagattt cataaagtat tttggaatgg gcttgatatt ttgtatgatg tttcgctaca
     4381 cccgatccat ctattgggct atagctctgc atgcttcatg gaatagtttc ttactcattg
     4441 ttactttatt ggtatttgga tattagaaag gtgatatatg aaaaagataa tctcacaccg
     4501 ctactttatt atagtttttc tactagttat tgctgaccag aagttcagtg ttctagtttt
     4561 acgcagcgac cttgttactg ggctatctga ctttgcctat tatctgtcgg atatgatgtt
     4621 gaattttctt gtggttttat ttgctcttat tgctatgatt tggtcgggga aatggcagaa
     4681 aatcaatagt agaaagttta aaggatccta tcttttctat tccttcctag ctctgcttgc
     4741 ttttgttgtt tggaatttcg ttaccttttt tcttttccca cctactcgaa atgaaatttc
     4801 ttatcaacat gctgctccga cttttacagg ggctacagca tttttgatgt atttttttta
     4861 tcctgtgatt gcaggtccca tttttgaaga catgatttat cgtggattag tgatgactgc
     4921 tctggaaaaa ggaaagaaat gggggctaga tgtacttggt tccgctgttt tatttggggt
     4981 ctcgcacatt agtaatcacg gttgggtctt gacagacttt gtcttctata tgggtggagg
     5041 tcttatattt gcagtcttat ttagaatgac aaagtcaatt tattggccta ttggactgca
     5101 tatagtctac aatggcattg gtcagctttt gatgttactg taattttggt tgttagtagt
     5161 atcgcggtct atccttgggg aaagattccc atttgaactt aagaaggagt tgtgatgaaa
     5221 gtatttcttc aaaatagaga ttttaggcaa ttaactatca accagtggat ttcaacgctt
     5281 ggggatacga ttttttatct ggccttcctg aattatgtgg cagatgcatc ttttgcccct
     5341 ttggcgattt tactcatcac gatttcagaa accctgcccc aagttctaca aatctttctg
     5401 ggagttttgg cggattttca acatcatcgt gtcttgaaat acacagtcat tagttttgca
     5461 aaatttttgc tttactctat agtgtcttta tcactttcag ggcagtcttt ctccttgttg
     5521 ctagtagcat ttatttgtct gattaacctc ttgtctgaca cattgagtta tttttcaggc
     5581 gccatgctca ctccgatttt cattagaatt attgggcaag accatctggc agaagctatt
     5641 ggctttaaac agtcaactgt tagtttagtg aaaacaatca gtaatatcct aggaggagtc
     5701 ttactaggca ttctatccat ccagtttatt tccttactga acgctcttac ctttttaatc
     5761 gcatttttag gtatcctctt cataaaaact gacctcttga aagtagaaaa aacgattagc
     5821 tatcaagaag gactctctgt aaaatccttt tgccagcatt tgctccaatc atcgaaattg
     5881 atatggaata tgaataaggt gctcttggtt ttgtttatta tctctactag tcaagcagtg
     5941 ataaatgtta cggttcctat ttccactcta tttttaagga accagccctt tttgaattta
     6001 caaactggtc aatctcttgc cttgttagcc acactagaat tatcagccct gattgtcgga
     6061 agccttgtaa gtggctatct gcaacatacc atttccataa aaacagctct ctatgcctca
     6121 cttgtcatcc agttgcttct tctagtagga tttgccacag ttcgttttga ctggattctc
     6181 atctttagta ccttggatgc ctttttcgca ggtgttctct ctcctagatt gcaagaactc
     6241 gtttttaaac aaatacctga ggagtcaatg ggagcagttc aatcctctat cggtgccatt
     6301 acggttgttt tacctagctt atttacaata gctttggtaa ccattgctac tagctttgga
     6361 actctggcag ttagctttgt tttattgcta tttcttctag ttgcctttgt tatgcttctg
     6421 aatattcgtg aaagtattta gtggagaatt tctataatat atgtttgaga ctggattgcc
     6481 agtcttttta tgatggtcaa aatcaacaga cttttttctc ttttctgaat gtgttataat
     6541 agactgaatc taaaatagta cgaaacaatt gctaaaacat ttatagaaat taattttact
     6601 ttcccaatcg atttgttctc atcttatttc aatctgctat ataatagtcc atgctgtggt
     6661 tggattcttt tcagcctact tgtactcttc gaaactctct tcaaaccacg tcagcttcac
     6721 tttgccgtag gtatggttac tgacttcgtc agttctatcc acaacctcaa aacggtgttt
     6781 tgagctgact tcgtcagttc tatccacaac ctcaaaacag tgttttgagc tgacttcatc
     6841 agttctatct gcaacctcaa aacagtgttt tgagcaacct gcggctagtt tcctagtttg
     6901 ctctttgatt ttcattgagt attattaaga caatatgagg agaaagatga aagaaaaagg
     6961 attttaggag ggggcgcagg cagccatgcc aacggctctt ggctatgtca gtatcggcct
     7021 ggcctgtgga attatcggtg cgccctatgt gacacctgtt gagatgggct tgatgagtct
     7081 ctttgtttat gctgggagtg cccagtttgc catgttggca ctgattgtgg ttcaagctcc
     7141 tgtggcagct attgctatga cggtttttct aatcaacttg cgtctctttt tgttgagttt
     7201 acacgcatcg acttatttcc gtcataccag tctttggtac aatatcggta tgtctagtat
     7261 cttgacagat gagacctatg gcgttttgat gggtgaattg gcccatacag acaaggtaaa
     7321 tcctatgtgg atgcacggaa acaatcttaa cagctatgtg gcttggtttg tggggacagt
     7381 agtcggaacg gctctgggtg gcctgctacc aaatccagaa atctttggct tggattttgc
     7441 cctggttggg atgtttattg gtatttttgc ttcgcaattt cagattatgc aaagacggat
     7501 tcctgtccgc aatctgctca ttatcctagc agttgttgcg gtgtccttct ttttgctctt
     7561 gacagtgatg tctcagtcac tagctgttct gtttgcgacg ctacttggtt gtagcatggg
     7621 ggtggtttta gatggtcagt aagtatcttt tattagcagt tattttctct ggcttggtga
     7681 cttggattcc ccgtatgatt cccttcatct tggtcaagta taagggcctg cctgcgattg
     7741 ttgagcgttt tttgaagttt ttgcctgttt ccattatctt tgccctgatt ctttcaagcg
     7801 tagtgacagg taaggttggc agtcttcctc aaatcaaatg actggacttc ttagccgtct
     7861 ttccaacagc ttgggtagcc tttcgctacc gcaatctagt cggaacagtt ctctttggag
     7921 tggtcttgat tgccatcttg cgtttggtct tttaaattac ccataaaaaa aacttatcac
     7981 agagatagat atcatataat ggcgtaaatg ctctttttct gttaagatta taaggtattc
     8041 tattttggag gaaatgacat gaaaaaaatc gttaaatact catctcttgc agcccttgct
     8101 cttgttgctg caggtgtgct tgcggcttgc tcagggggtg ctaagaaaga aggagaagca
     8161 gctagcaaga aagaaatcat cgttgcaacc aatggatcac caaagccatt tatctatgaa
     8221 gaaaatggcg aattgactgg ttacgagatt gaagtcgttc gcgctatctt taaagattct
     8281 gacaaatatg atgtcaagtt tgaaaagaca gaatggtcag gtgtctttgc tggtcttgac
     8341 gctgatcgtt acaatatggc tgtcaacaat cttagctaca ctaaagaacg tgcggagaaa
     8401 tacctctatg ccgcaccaat tgcccaaaat cctaatgtcc ttgtcgtgaa gaaagatgac
     8461 tctagtatca agtctctcga tgatatcggt ggaaaatcga cggaagtcgt tcaagccact
     8521 acatcagcta agcagttaga agcatacaat gctgaacaca cggacaaccc aactatcctt
     8581 aactatacta aggcagactt ccaacaaatc atggtacgtt tgagcgatgg acaatttgac
     8641 tataagattt ttgataaaat cggtgttgaa acagtgatca agaaccaagg tttggacaac
     8701 ttgaaagtta tcgaacttcc aagcgaccaa caaccgtacg tttacccact tcttgctcag
     8761 ggtcaagatg agttgaaatc gtttgtagac aaacgcatca aagaacttta taaagatgga
     8821 actcttgaaa aattgtctaa acaattcttc ggagacactt atctaccggc agaagctgat
     8881 attaaataat ttcttgaaat catccattct gagtaaggtg ggtggtttct cagtttttag
     8941 ggatatagtt ttaaactata tatctgacta tctaataaca atttgtgaaa tcaaacaaat
     9001 tgtgagatac tagtacggta ttatttttaa ggagaaagaa tcatgaaaat caaaaaatgg
     9061 cttggtctag cagcccttgc tacagtcgca ggtttggctc ttgcagcttg cggaaactca
     9121 gaaaagaaag cagacaatgc aacaactatc aaaatcgcaa ctgttaaccg tagcggttct
     9181 gaagaaaaac gttgggacaa aatccaagaa ttggttaaaa aagacggaat taccttggaa
     9241 tttacagagt tcacagacta ctcacaacca aacaaagcaa ctgctgatgg cgaagtagat
     9301 ttgaacgctt tccaacacta taacttcttg aacaactgga acaaagaaaa cggaaaagac
     9361 cttgtagcga ttgcagatac ttacatctct ccaatccgcc tttactcagg tttgaatgga
     9421 agtgccaaca agtacactaa agtagaagac atcccagcaa acggagaaat cgctgtaccg
     9481 aatgacgcta caaacgaaag ccgtgcgctt tatttgcttc aatcagctgg cttgattaaa
     9541 ttggatgttt ctggaactgc tcttgcaaca gttgccaaca tcaaagaaaa tccaaagaac
     9601 ttgaaaatca ctgaattgga cgctagccaa acagctcgtt cattgtcatc agttgacgct
     9661 gccgttgtaa acaatacctt cgttacagaa gcaaaattgg actacaagaa atcacttttc
     9721 aaagaacaag ctgatgaaaa ctcaaaacaa tggtacaaca tcattgttgc aaaaaaagat
     9781 tgggaaacat cacctaaggc tgatgctatc aagaaagtaa tcgcagctta ccacacagat
     9841 gacgtgaaaa aagttatcga agaatcatca gatggtttgg atcaaccagt ttggtaataa
     9901 gaaacaggga ggtgggagag aaaattccac ctcttgcttt tgtatagagt atagattgta
     9961 aagaagacta ttcgttcata gaaaggtaga gagaatatgg tttttcctag cgaacaagaa
    10021 cagattgaaa aatttgaaaa ggatcatgta gcccagcatt attttgaggt tttgcgtacc
    10081 ttgatttcta agaaatcagt ctttgcccag caggttggac tcaaggaagt cgcaaattat
    10141 ctgggtgaga ttttcaagcg tgttggagct gaagtggaga ttgatgagag ctatacagcg
    10201 ccctttgtca tggcacattt caagagttcg cgtccagatg ccaagacctt gattttctat
    10261 aaccactatg acactgtgcc agcggatggg gatcaggtct ggacagagga tccttttacg
    10321 ctttcggtcc gcaatggctt catgtatggg cgtggggttg atgacgacaa gggtcatatc
    10381 acagctcgct tgagtgcttt gagaaaatat atgcagcacc atgatgattt acctgtcaat
    10441 atcagcttta tcatggaggg agcggaggaa tcggcttcaa cagacctaga taagtatttg
    10501 gaaaagcatg cagacaaact ccgtggggcg gatttgttgg tctgggaaca agggaccaaa
    10561 aatgccttgg aacagctgga aatttctggt ggcaataagg ggattgtgac ctttgatgcc
    10621 aaggtaaaaa gcgctgatgt ggatatccac tcgagttatg gtggtgttgt ggaatcagct
    10681 ccttggtatc tcctccaagc cttacagtct cttcgtgctg cggatggccg tatcttggtt
    10741 gaaggcttgt acgaagaagt acaagagccc aatgaacgag aaatggcctt gctagaaact
    10801 tatggtcaac gaaacccaga ggaagttagt cggatttatg gattggagtt gcctctctta
    10861 caggaggagc ggatggcctt tctaaaacgt ttctttttcg atccagcgct taatatcgaa
    10921 ggaatccagt ctggttatca aggtcagggt gttaagacta ttttacctgc agaagccagt
    10981 gccaagctag aggttcgtct ggttccgggc ctagaaccgc atgatgttct ggaaaaaatt
    11041 cggaaacagc tagacaaaaa tggctttgat aaggtagaat tatactatac cttgggagag
    11101 atgagctatc gaagcgatat gagcgcacca gccattctca atgtgatcga gttggccaag
    11161 aaattctatc cacagggcgt ttcagtcttg ccgacgacag cggggacagg acctatgcat
    11221 acggtctttg atgccctaga ggtaccaatg gttgcattcg gtctaggaaa tgccaatagc
    11281 cgagaccacg gtggagatga aaatgtgcga atcgctgatt attacaccca tatcgaatta
    11341 gtagaggagc tgattagaag ctatgagtag agatattatc aagttagatc agatcgatgt
    11401 gacttttcac caaaagaaga gaaccatcac agcggttaag gatgtgacca ttcacatcca
    11461 agaaggggat atctacggaa tcgttggata ttctggagca ggaaaatcaa cccttgtacg
    11521 ggtgattaat ctcttgcaaa aaccatctgc agggaaaatt accattgacg acgatgtgat
    11581 ttttgacggc aaggtgacct tgacggcaga gcagttgcgt cgtaaacgtc aagatatcgg
    11641 aatgattttc cagcatttta acctgatgag ccaaaagaca gcagaggaga atgtagcctt
    11701 tgcccttaaa cactctgaac tcagcaagga agaaaagaag gctaaagtag ctaagttgtt
    11761 ggacttggtt ggtttggcag atcgtgctga aaactaccct tcacaactat ctggagggca
    11821 aaaacagcgt gtggcaattg cgcgtgcctt ggccaatgat ccaaaaatct tgatttcaga
    11881 cgagtcaact tctgcccttg atccgaagac aaccaagcag attttggcct tgttgcaaga
    11941 tttgaaccaa aaattaggct tgactgttgt cttgattacg catgaaatgc agattgtcaa
    12001 agacattgcc aaccgtgttg cagttatgca ggatgggcat ttgattgaag agggtagtgt
    12061 gcttgaaatc ttctcaaacc ctaaacaacc tttgactcaa gactttatct caacagctac
    12121 aggtattgac gaagccatgg tcaaaatcga gaagcaagaa atcgtggaac acttgtctga
    12181 aaacagtctc ttggtgcaac tcaagtacgc tggagcttca acagacgagc cacttttgaa
    12241 tgaattgtac aagcattacc aagtaatggc taatattctc tatgggaata tcgaaattct
    12301 cgatggtact cctgttggag aattggtggt ggttttgtca ggtgaaaaag cagcgttggc
    12361 aggtgcccaa gaagccattc gtcaagcagg tgtacaacta aaagtattga agggagtaca
    12421 gtaagatgga atcattgatt caaacctatt taccaaatgt ctataagatg ggttgggctg
    12481 gtcaggcagg ctggggaacg gctatctact taactcttta tatgacagtt ctttccttca
    12541 ttatcggagg cttcttgggg ctagtggcag gtctctttct cgtcttgaca gcgccaggtg
    12601 gtgtcttgga gaataaagtc gtattctgga ttttagacaa aattacctca atttttcgtg
    12661 cggttccctt tatcatcctc ttggcaatct tgtcaccact ttctcacttg attgttaaaa
    12721 caagtatcgg gccaaatgca gcccttgtcc cactttcttt tgcagtcttt gccttctttg
    12781 cccgtcaggt gcaggttgtc ttggctgaat tggatggcgg tgtcattgag gcggctcaag
    12841 cgagcggagc gactttctgg gacatcgtgg gtgtttacct atcagaaggt cttccagatt
    12901 tgatccgtgt gacgactgtg accttgattt cccttgttgg ggaaacagct atggccggtg
    12961 cggttggagc tggtggtatc ggtaacgtag ccatcgctta tggatttaac cgctacaatc
    13021 acgatgtgac catcttggca accattatta tcattttgat tatctttgca atccaattct
    13081 tgggagattt cttgactaag aaattgagcc ataaataaaa aagagccgcg tggctctttt
    13141 taactgatca gattttctgg gcaaattttt tactcaaggc ttgtccaatc aaggcaccca
    13201 ctagggctcc gatgacaata cttgcgataa atagaaggac agttccaggg tttggagcga
    13261 ccatgatacg gtcgatatat tcttgggatt ttcctcttgc cagaagagta gccatatagg
    13321 ctttgggcgc aatccacata agcaagattg gtcctgttgt actaaaggcg aaaataatga
    13381 aagaaaggaa gttctttgtt ttgtccttgt attttcctaa atgagctact ccatctgcta
    13441 ggaggccaca gataattcca ggaaggaagg aaccagcacc gtgtttagtt cccaagaaaa
    13501 agagggcaat gacaaggccg atagtggtaa tggctccaaa gcgcggaact tttgcgacta
    13561 ggatcatata gacgctaccg ccgacaaggg cagtaaaggc aggcgcataa aacatgtttc
    13621 cagtttggtc aaagagattg cccaaaagga caccaatccc catgcagagg aagtaaagaa
    13681 ctgcaaaaag cagtgtagtt aagatagatt tcttcatgaa ttgtctcctg ataatttttt
    13741 cacaattctc attttatcat ggtttggtgg gattgtaaat ggatactaga agtttttgaa
    13801 aacgcttgaa atatgatata atggtttcat agttattttt aggggaatat catgggtaga
    13861 tttttagact ttgtctttaa tcgtttcttt ttagggatga ttgcgacagc cttcttttgg
    13921 ctattaactt tagcaggagg gattatcctt ggtctagcgc cggctagtgc caccttgatg
    13981 agcttatatg cagaacatgg ttatagcttt cgggaataca gtttgaagga ggcttggtct
    14041 ctttacaagc aaaattttgt ctcaagcaac ctgattttct atagcttttt aggtgtgggt
    14101 ctagttttga cctatggttt gtatctcttg gtgcaattgc ctcatcagac cattgttcat
    14161 ttgattgcga cccttttgaa tgtcctagta gttgccctga tctttttggc ttatacagta
    14221 tctttaaaat tacaagttta ttttgccttg tcctatcgaa atagtctcaa attatccttg
    14281 attggcatct ttatgagtct agcagctgtg gctaaggttc tccttgggac tgtgctactt
    14341 gtagcaattg gttattatat gcctgccctg ctattttttg taggaattgg gatgtggcat
    14401 ttctttatca gtgatatgtt ggaacctgtc tatgaaatca tccatgaaaa attggcgaca
    14461 aaatagaatg aagcagtttt ggctacatac gcttctaaga acctatagtt cagtgatgat
    14521 cattatcatt gcgagttttg caatcttact ctcttacgct gactgggatt cacgtgaaaa
    14581 ggaagcccag agagtagccc agcgtgtaac tgctagaaca gtgagtgaaa ttgaatatta
    14641 ccatagagag tcaacccaga tagctcaggc tttagttgaa aatcaagctc gtatcgaggg
    14701 aatctataaa tactttagcc ttagcatgcc agactatttt tactggcaat tagagcggaa
    14761 agcttcgcct tatatatcag tctctctgta tgaaaatgtt gatgacctct atgttcgaaa
    14821 tgattttgta actggggtgg ccattgcttt tcaagattac aaggaagtct atgtttctac
    14881 taaagacaaa cgtagtggag aaaaaatcag ggctgaggat ttcaaaccag caggaaatag
    14941 ttttgccatt ccagtgtcag atccagtgtc agatcaagac ttaggagtga tttacatctc
    15001 cttggatcct gctgttttat accatgccat tgataatact agaggtcata ctccgatggc
    15061 agtaacagtg acctcacctt ttgatacgga gatttttcat atgggtgaga cagttgataa
    15121 ggagagtgaa aattggctag ttggcttaac ttctcatgga tatcaggttc aggtggcagt
    15181 tcctaaaaac tttgttttac aaggaacagt gactagctct gctttgattg tgggtttgag
    15241 ccttctcttt attgtcattc tttatctgac tttgaggcag acttttgcta attaccaaaa
    15301 gcaggtagtg gatttagtag aatccattca agtcattgct caaggcgaag aggggcgtcg
    15361 gattgacatt tccgagaaag atcaggaatt actcctaatc gcggagacga ccaatgatat
    15421 gttggatcga ttggaaaaga atatccatga tatttaccag ttagagctta gtcaaaaaga
    15481 tgccaatatg cgagccttgc aggcgcaaat caatcctcat tttatgtata atacgctgga
    15541 gttcttgcgc atgtatgcag ttatgcagag tcaagatgag ttggcagata tcatttatga
    15601 attcagtagt ctcttgcgta acaatatttc cgacgaaaga gagaccctcc tcaaacagga
    15661 attagaattt tgccgtaaat acagctatct ctgcatggtt cgctatccca agtccattgc
    15721 ctatggtttc aagatagatc cagagttaga gaatatgaag attcccaagt ttaccttgca
    15781 accgctggta gaaaactatt tcgcgcatgg tgttgaccac aggcggacag ataatgtgat
    15841 tagcatcaag gctcttaaac aggatggttt tgtggaaatt ttggtggtcg ataatggtag
    15901 aggaatgtcg gctgaaaagt tggcaaatat ccgagaaaaa ttaagtcaga gatattttga
    15961 acaccaagcc agctacagtg atcaaaggca gtctatcggg attgtcaatg tacacgagcg
    16021 ttttgtgctc tattttggag accgctatgc cattactata gagtctgcag agcaagccgg
    16081 tgttcagtat cgtattacaa ttcaagatga gtagaaaggg agaaaatgta taaagtatta
    16141 ttagtagatg atgagtacat ggtgacagaa ggtctgaagc gtttgattcc ctttgataag
    16201 tgggatatgg aggtcgtcgc aacagccagt cattccgatg aagctctaga atatgttcag
    16261 gaaaatcctg tcgatgtcat catttccgat gtcaatatgc cagacaaaac agggcttgat
    16321 atgattcggg agatgaaaga gatcttacca gatgctgcct atatcctgct ctcaggttat
    16381 caggagtttg attatgtaaa aagagcaatg aaccttagtg tggtggacta tttggtcaag
    16441 cctgttgata aggtagagct gggaaatctg ctggagaaga ttgcaggtca gctcggcgag
    16501 agagggaaga aaagtcagac tcttagtcaa gaattagacg aggctggatt tgttagttat
    16561 ttaggggata aggagaattg gtggataggt ctatccaagg aaaaacaagg ttccttcacc
    16621 attccctact atgtcttggg tcaagactgg cagattttca tttctggcca ccccctagat
    16681 ggtttagtcg ttacaccttt tgaagctcct tatcaagaac actttgaacg ctggaagctg
    16741 aatgctgaga aaaccctctt ttacggttct gtaaatctgc agcagtctga gagtctcttt
    16801 gcctattacg aaccgattta tagggttatc attcagggaa atctcaatca aatcgtagaa
    16861 gagttaaatc tcttggagaa ggtagttctt gaaaatacac ctcgtgtttc gattactaaa
    16921 cagcttttta tccagtttgt catggatgtt ttccatttat ttgaacatct caaagctgat
    16981 gatatgacgg acattgtcaa aaccattcat gctattcaat ccttcgatga attggtttct
    17041 tatatcaagg aaactctgat cagctttttc ggtcaatacc gtatgaatga aaatgtggtc
    17101 agtgtgctgg aagtcattgg tcgtgattac caaaaagagc tttccctcaa ggatatcagt
    17161 aaggccctct ttatcaatcc tgtctatcta gggcagttga ttaagcgtga aaccgattcg
    17221 acctttgcag agttactaaa caaacaacgt attaaggctg cccagcagct cttgctttca
    17281 actagtgaca gtatcgaaga tatttgttat gctgttggtt acagtaacct tggatatttc
    17341 tataaagttt tccgaaaatt gtgcggaaaa tcgccaaaag cctaccgaaa acaggtagaa
    17401 actatactat aagatttgta ttcctttaca aaaggtgcta taatattatc aggaggtcct
    17461 atcatgaaaa agaaaccaat ttatctatgg gttttgctaa tcttgtctgc ccttatttca
    17521 gttccgtctc tgtttggaat tgtgagtccc ttgcctagta aagaagccct tcgtgctgct
    17581 cagaaacaag ttgcaggggt caatgctcag caattagaag accagcttaa ttatacctat
    17641 agagtagcag aagcatctca ttctattttt aatgttgctt tgattgtgct atctactatt
    17701 ttagtggtcg tagcgattgt cttccttgtt cgtaaaaatt tgcaatatgc aaactatact
    17761 tacgttggct atgttttgtt agctattatt ggttcgattt atggctatgt tggtttacaa
    17821 gacgctgtgc agttggttca agatgagagc atgcgtttga cagtgagtat tggttcaaaa
    17881 gctgttagca ttttctatat cgtcatcaat gttctcttcc tagcccttgt cttctataag
    17941 atgtggcgac aacagaaagc cttggcagaa gaagaggaaa cagaagagct tacctaagta
    18001 ttgacaaaaa agaactatca agatacaatc ttggtagttg ttttttgatt atagataaaa
    18061 tcatggaggt acatatgaag acaatttctt tagtttatat tagtttgagt ggcaacactg
    18121 aaagttttgt gacgcgcttg aaagactatc tcttgtccca gtacaaaggg attgaggttc
    18181 aaaagattca tatcaaggat ttggtcaagg aaggcaaaaa tttctatgaa atggaccatc
    18241 cctatgtcgc ttttttgccg acctatctcg aaggtgggaa tggcgtggat aacggagatg
    18301 ttgagatttt gacgacaccg gtgggagatt ttattgccta tggtaacaat gctagtaagt
    18361 gttttggtgt ggttgggtca ggaaatcgta actttaataa ccaatactgc ctaacagcca
    18421 agcaatacag tcaacgcttt ggcttccctg tattggctga ctttgaaatg cgaggcatgc
    18481 tggaagatat caaacatgtc gcagctatta tcgcagattt gtatgagttg gaaaaggaga
    18541 attaatactc aatgaaaatc aaagagcaaa ctaggaagct agtcgcaggt tgctcaaagc
    18601 acagctttga ggttgcagat agagctgacg tggtttgaag agattctcga atagtataaa
    18661 gttcttataa gaggtggttg aagttgttca actgtttttt gagtataaac agtctttaag
    18721 aacgtaaaaa agcataagtt caggtcttca aaaccttgtt cttatgcttt ttatcataga
    18781 aatatttgct gaattttctc ttgaaatgaa accttatctt catctcgcgc ttgtccctta
    18841 ccctgctttt ccgattgcga gtgcgtagat aaagaagatg gcgaagccaa agaggaaaat
    18901 caatcccgca atggcaaggt gtttgagcca agaaggaaga tttttgtttt cacgcgttac
    18961 caaggcgtaa ttcaaaagag caaagaaagg tgttgtcagg aaagagccaa tcatggcaaa
    19021 gcggagcatg gttgaaacct gaccagcgaa gaacttgata atgacgatac cgatgatagc
    19081 agtgatggtc atccagatgt tcaaagattt acgattgtcc tctttttgac tgattagcag
    19141 tcggagagat tcctgattaa cgcgagaata gccatcgata acagttataa ctgttccaaa
    19201 gatacagagg aaggcaataa aggtaatcaa gtaacgggac cattcgccaa gaacagaggc
    19261 atacatgccc acgaattgag agatgtattt ggctgaagca gcttcaaccg cctgccctgt
    19321 aggatactga atcagtgctc ccagtgccac aaagaagacg gctaggatag ctgttccaat
    19381 ataaccagtg ttaaagtcaa acagagcgtc ttctgtgtta aagttgacgg tctttctctt
    19441 ttcagctgac caaagtgaat tgatggctga aatttcaata ggagccggca tccatcctag
    19501 gagggagacg atgaagggca gagctgccat ttgccaaggt gtcttctcga caaaatcaga
    19561 actgtattct ggatgcttga ccgccgcaat gataactgca agaacagtcg caatggttaa
    19621 ggcagacata atccatttga ccatgccgtc taaaagtttg tagcctccaa agagtagcat
    19681 agcccaaatg attgcaacga gaatgaggga ccactgagta atgctaagtc caatcattgg
    19741 gaaggcactg gcgatgatag ctgagcacag aatggcaaca ccagccgtgt tgaccatagc
    19801 cgaaaagaca ttgaggataa agaaaatcca gagatagagt tttccttttt cggcataacc
    19861 ttcaaccaaa gtctttccag tatcagctgt gtattcagca ccaaaacgga aaaatggata
    19921 tttaaagaca ttggctaaga tgaccaagag aagtagagac caaccgtaag aaccgccagc
    19981 ttgagttgag gatacaatgt gggaacctcc aacagcggca gttgccatta ggattccagg
    20041 tcccattgct tttagcttgc ttgcccaggt tgattgattt aaggaaatag cttgcgacat
    20101 gataaatact cctttttgaa ttttcagaat aatctgtata tgtaatctat attctacaaa
    20161 aaattttgga aaaatgcaag aatattttga aaaaagatag aaaatttaca aagaaaatct
    20221 gaaataactg ttatgatata gcagattgaa actagaatag tacacctttg cttctaaaac
    20281 attgttagaa atcgatttga ctgtcctgat cgatttatcc tgttcttatt tcatttcact
    20341 atagtaaggt gcagcaggat aaagaaaaac cgagaagttt ctttctcgga tttgcttatt
    20401 atgaggttcc tattttctat gctccaagag ttgattgatg tgatctactt ttttagcctc
    20461 tcttttatag agaatagccc agatgatgag atagacaatc gcaaactcga taatgagctg
    20521 gaggtagaaa atccagtgga aagggaacca accagcaaga gttgctagtg ggacaaagcc
    20581 agccagcata aggaagaaat gagtcagagt ggcacgaagc atgctccagt cacggctaaa
    20641 taagcggttg ccaaagttga agagcatacc gatggttgcc cagataagtg tgcagtagag
    20701 caagaccagg gcaccgtgaa cctgatgctg atccatcact tggcctatga gagagtaggg
    20761 atttagtggt gcgtaggtat ttggtgcata aatgagtgaa aagaggatag agaggatgag
    20821 gccgataaga acaccggtag ctgcatcgtg aaatacttgt tttttcatag ttctaatttc
    20881 tccttgatgg tttttagata acggcgtgaa gagtaggtga agctttcgtt tttcaagaaa
    20941 atttctacca gaccgtttgg cgtgagcttg aggtgagaga tggaatcgat attgatgatt
    21001 tctgattggg aaatttggat aaaattggtt ggcaagagtt taagaacctg atagagtcgc
    21061 aaatcaatgc tgtaggtctg actcgcggtt tctgctagaa ccttccgatt ctcgatatag
    21121 aagcgctgaa tcttgccaat ctcaactaga tagacctgat catcgatttt tcctttgatt
    21181 ttttctcttt ggtccagatt ttctgcgaac tcgatgactt tctggacttt ttcggtttct
    21241 tgaggtgctt ggacaatcag cttttcctcc tcgtaagtct cactaatctg tagttctact
    21301 ttcatagttt tctctccttt tcagttatac aaggttgtga tcacttcctg tatatcctta
    21361 ttaaacacta ttttatagcc catggcaagg aaccttgtct atgtggaggg attgggctcc
    21421 tatgtggtgg agcttttctg ttctttctga aatatggtat aatagcacta atcaatttct
    21481 aggaaaatag atacggaaag gggctgaaag aactagggga acagttggaa ttagaaagtg
    21541 ataggtatga aaagtggcgt tgaagctgaa taagtttata aagcgtttga atgaagaaat
    21601 ggaaagtaaa taagttgacg atgaattaca aaatagattt tatctacaat ttgtatcttt
    21661 caaatcattg atttgaattt tagatttaat catgttacaa ttaaaactga aatcaaatag
    21721 aagaggaaaa tgcaaacgtg ggcacactat tagcaacaag attaaaaaat agacggaaag
    21781 aattaaaaat gtctcagcga gaactagctg aaggtatctg taaacagggg caaatcagtc
    21841 gattagagag cggagagttt acccctggag cggatttcct gaatgccctt gctaagaagt
    21901 taaaagtcag tatggattat ttttttgatg agcaggttgt agagaaagtt gaggagttat
    21961 cagaatttaa gaaattagca cagacattta ttacaaatcg caattatgag tctttgaaat
    22021 atatatatga attggagaat gtaaaagctc accgcctatc tttagcagat aaaatctaca
    22081 tggaatggat aaaatctctg atagattttt atttttacgg acagaaagaa gatgcgattg
    22141 tacgattaga gggggtatta tcacagttaa gtgtatctga tttgaactat ctccaagttt
    22201 caaacaccct attcaatttt tattatgata ttggagattt aggtcgtttc aataaaattc
    22261 gtgaaaaact agagtatcaa gtaaatcacc tcaatttgag tacaatcgaa gagttagaac
    22321 tatctattaa gtttaactac aacgtttgtc gttatctatg gttacagaaa aatattgaag
    22381 aagctattac taaaattaca gctactataa aacaatgtaa ggcttacaga acgaattatc
    22441 ttttggctga tttgtactta ttgatgggaa atgtaagtga aaatttttct tctaaaagtt
    22501 cggtgaaaga atattttgag acggcatatt ttctatacaa acttgaggag aacatgtcaa
    22561 tgactctgaa agttgagcat tacattgcag atataacaga ataatgaata cggtgttcaa
    22621 gtgagcatcg tttttcaaac ctagttcaga tacaaaaaag tatcaaacaa aaaaaaataa
    22681 aaaaacatta taaaaacatt ataaaaacaa ttgacaagct aattttgaag tgataaaata
    22741 aatgcaagaa aacaaaagga gaggtatatg agaagtttgt ttaagaaaat agttgctgta
    22801 ttagttattg gcttaattct gttaggaatt gcgagaacac ctcaagttca taagatggct
    22861 agagggattg atcctggtcc tgcgaatttt atttagaaat caatttctac taattgagta
    22921 ttaatgggta aaaagattgt atggataaga aaaatttact tgtattggtt actggttcgg
    22981 taggtgccag taatgtagat acatacttat attacataaa aaaattctat aatgttaaag
    23041 tgatactttc tgaaaattct aaaaaattta taagtaagga attaattagt tatttttgtg
    23101 ataaagttta tgatgaaata tttgttgaag aggtagttcc gcacgttttt ctgccatatg
    23161 aatctgactt acttcttatt ttaccagcta cggcaaatgt gattggcaaa attgctaatg
    23221 gtattgctga tgatttagtt acagcaactg ttttaaactt taataaaaaa ataatttttt
    23281 gtcccaatat gaactctact atgtgggaca atcacatagt tcaaagaaat gtatcaattc
    23341 taaaggagtt gggacatata tttttatttg agtctaaaaa aacatatgag gtaggattgc
    23401 gtaaagcaat agattcaaca tgttcaatgt tacaaccaca gtcgttagta aaagaactta
    23461 tcaaattaga aaatattgtc cttgaagagg gacattaaaa actactgaga atattaatga
    23521 ggggaaaaaa tggaaaattc atcaatcgat gtagatatgc tgttggaaga attgacacaa
    23581 gaagcaatgg tcgttgttgc tgttgataag gactgttaat ttaaacttat ggcaatatat
    23641 gaaaggttac tggatgtttt aaattatgca ggcagtagcc ttttattata tacaaatgga
    23701 taaagtaagg ataatacaat gattaataaa aaaatacaac aagttgtttt ggaatcatta
    23761 cagaattttt tgaatgggaa cttcatttcg ccttgtgtag tctatgattt tggcttgctg
    23821 gaaactgtac ttgatgaatt taaaaatcaa attcctgtaa cattcaatta ccaacttttt
    23881 tatgccgtta aagcaaattc aaatgagaag atacttgaat tcttagtaga taaaattgat
    23941 ggagttgatg tggcgtcatt atctgaatta gatgtggcta aaaaattttt cccaccaact
    24001 caaatttctg ttaatggtcc cgcattttct tatgaaactt tatataatct gattaaaaaa
    24061 caatataaag ttgatattaa ctttttggaa catcttcaac aattttcccc aaaagaatct
    24121 gttggaataa gagtaacgga gccagatgaa cttaataatc gtatgagtcg atttggaata
    24181 aatatttgca gtgataattg gactagtaat ttacaaaatc ctttaattac acgactgcat
    24241 tttcattttg gagaaaaaga tgataaattt attgttaagt tagataaaat attatttaag
    24301 ttacaagaaa ttaataaact tagagaggtt agagaaataa atcttggagg cggttttatg
    24361 aaattattta tggaaaatcg tttgaaagaa ttttttctat cacttatgga aatctataaa
    24421 aagtacgata ttgatagtac tgtgactaca ataatagaac caggtagtgc aattacttca
    24481 ttttctgcct atatgattac tagcccagtt aatgttagtg aggtgaatga gcagcaggtt
    24541 atcacgttag acacatcaat atacaccaat acattatggt ttgttccgca tattattaca
    24601 acgttaaatt caagtagtaa agagcgttat agtactattc tctatggtaa tacctgttat
    24661 gaacatgaca agtataaaat gaaagtttcg cttccaaggt taactcaaaa tagcagtata
    24721 gtgttttttc ctgtaggagc ttatataaaa agcaatcatt caaatttaca tcgtaatgat
    24781 tttatgcggg aggtatattt gtggacaaaa aacttgacat attagataaa gttaaggaat
    24841 atttaggaaa taaaactact caaattctgg ataatcaata taaagaattt ttgaaactta
    24901 atgatataag gcgagcgttt ggtatttcag aaaaagtatt aaacaattct tttaatttta
    24961 cgagtaaaga atttaatgat ttaattaata acgaaaatta tttattcgaa tatgcatgta
    25021 gaaagagagg aatggagaaa aaaatgcttt aatcattctt atcgttttct atgctcacct
    25081 ataattacag atgattttct taacacgaag acattgagaa gtagccaaat tgaatataaa
    25141 tatgagcgat atttatcgaa aagttcgata ggcgatagag cggttgatgg ctttgtttcc
    25201 ttcaatactt taacagctaa tggtatgtct gctattaaac tatgtcttga gatattaaac
    25261 tctattttct tcaagaagaa gattgattta ttatattcaa ccggatatta tgaaacaaga
    25321 tttttattaa ataatcttgc taaatcaggt attagttgct atgaggtaag taattgtgaa
    25381 ttggataaag ataaatttta taatgtattc atgatggaac
//
LOCUS       DAVKNL010000027        24981 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVKNL010000027 DAVKNL010000000
VERSION     DAVKNL010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24981
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.027"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(346..1245)
                     /gene="galU"
                     /locus_tag="VW411_001125"
     CDS             complement(346..1245)
                     /gene="galU"
                     /locus_tag="VW411_001125"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEU9793860.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDKTTDMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FEILEKQAPGAGNEIQLTDAIDTLNKTQRVFAREFKGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(1267..2283)
                     /locus_tag="VW411_001126"
     CDS             complement(1267..2283)
                     /locus_tag="VW411_001126"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU9793861.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQKLFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            2495..2876
                     /locus_tag="VW411_001127"
                     /pseudo
     CDS             2495..2876
                     /locus_tag="VW411_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809823.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(3050..3475)
                     /locus_tag="VW411_001128"
     CDS             complement(3050..3475)
                     /locus_tag="VW411_001128"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9793862.1"
                     /translation="MTNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKSLTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTCLTE"
     gene            complement(3543..4193)
                     /gene="phoU"
                     /locus_tag="VW411_001129"
     CDS             complement(3543..4193)
                     /gene="phoU"
                     /locus_tag="VW411_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU9793863.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(4208..4960)
                     /gene="pstB"
                     /locus_tag="VW411_001130"
     CDS             complement(4208..4960)
                     /gene="pstB"
                     /locus_tag="VW411_001130"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9793864.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(4962..5777)
                     /gene="pstA"
                     /locus_tag="VW411_001131"
     CDS             complement(4962..5777)
                     /gene="pstA"
                     /locus_tag="VW411_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU9793865.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(5770..6633)
                     /gene="pstC"
                     /locus_tag="VW411_001132"
     CDS             complement(5770..6633)
                     /gene="pstC"
                     /locus_tag="VW411_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU9793866.1"
                     /translation="MTKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFIANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(6751..7626)
                     /locus_tag="VW411_001133"
     CDS             complement(6751..7626)
                     /locus_tag="VW411_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEU9793867.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(7793..9124)
                     /locus_tag="VW411_001134"
     CDS             complement(7793..9124)
                     /locus_tag="VW411_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEU9793868.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGLGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(9125..9832)
                     /locus_tag="VW411_001135"
     CDS             complement(9125..9832)
                     /locus_tag="VW411_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9793869.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(9916..10401)
                     /locus_tag="VW411_001136"
     CDS             complement(9916..10401)
                     /locus_tag="VW411_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU9793870.1"
                     /translation="MAPTDKEDLIRTLELAPHIEGGYFRQTKKASDTINLAGKGRALY
                     TSIYFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQL
                     HYCVPKGTIWGSTVDKDDALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTR
                     Y"
     gene            10387..10944
                     /locus_tag="VW411_001137"
                     /pseudo
     CDS             10387..10944
                     /locus_tag="VW411_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(11259..12950)
                     /locus_tag="VW411_001138"
     CDS             complement(11259..12950)
                     /locus_tag="VW411_001138"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEU9793871.1"
                     /translation="MNTKELIASELSSIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGIGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            13086..13532
                     /gene="argR"
                     /locus_tag="VW411_001139"
     CDS             13086..13532
                     /gene="argR"
                     /locus_tag="VW411_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9793872.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            13583..16117
                     /gene="mutS"
                     /locus_tag="VW411_001140"
     CDS             13583..16117
                     /gene="mutS"
                     /locus_tag="VW411_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEU9793873.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            16264..17958
                     /gene="patA"
                     /locus_tag="VW411_001141"
     CDS             16264..17958
                     /gene="patA"
                     /locus_tag="VW411_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEU9793874.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQDGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(18289..18558)
                     /locus_tag="VW411_001142"
                     /pseudo
     CDS             complement(18289..18558)
                     /locus_tag="VW411_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            18750..20516
                     /gene="patB"
                     /locus_tag="VW411_001143"
                     /pseudo
     CDS             18750..20516
                     /gene="patB"
                     /locus_tag="VW411_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
     gene            20655..21344
                     /locus_tag="VW411_001144"
     CDS             20655..21344
                     /locus_tag="VW411_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU9793875.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            21348..21527
                     /locus_tag="VW411_001145"
     CDS             21348..21527
                     /locus_tag="VW411_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793876.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            21514..22863
                     /locus_tag="VW411_001146"
     CDS             21514..22863
                     /locus_tag="VW411_001146"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEU9793877.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     SIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            22987..24447
                     /locus_tag="VW411_001147"
     CDS             22987..24447
                     /locus_tag="VW411_001147"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU9793878.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREEFIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDEFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            24461..24895
                     /locus_tag="VW411_001148"
     CDS             24461..24895
                     /locus_tag="VW411_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU9793879.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAGDGQIFTGF
                     CMEGTCGVFHLCAERAALFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGKERASKFNER"
BASE COUNT         7436 a         4885 c         5326 g         7334 t
ORIGIN      
        1 ttttattaat agcttccaca aaattaaaca acttatgttc ggctcggttg atttaattta
       61 accatgacta ctccaacagt taagtcaaat ttaaaattat ttattatttt ggaaccatta
      121 gaaacaacat agcaaagtta aaataaggct ttgtccgtac acaacttgaa aaagtgcgca
      181 ccgattcggt gcttttttgt tttactatac taatttattc tactcttatt ttaatgaaaa
      241 aacaaataca agtatagcgt tttcattagt ttttattaaa taggtagatt caagtagaga
      301 atttacttaa tttatgtgtg gctaatcttt atataaatga ttttgttatt ccttctcagt
      361 caattctttt ccaagttgga tgaggtaatt cttcaaatca tctttgactt gtgggtgttt
      421 gagggcgtag tcgatggatg ttttcatgaa gccaaacttg tctccgacat cgtaacgagc
      481 ccctttgaac tcacgagcaa atacacgttg tgttttattg agggtgtcga ttgcatctgt
      541 cagctgaatt tcatttcctg cacctggagc ttgcttttcg agaatctcaa aaatttcagg
      601 cgtgaggagg tagcgtccga taatagcaag gtcgctagga gcgtcctctg gagctggttt
      661 ttcaacaaag gtttcaacac tgtaaagacc atcttttcct tcgccttgcg gagcaataac
      721 cccgtaagca gatacttcgt catgagggac tggcatgaca gcgatagtag acgcgtgggt
      781 acgctcgtag tcatccatga gttgtttggt aagtggaaca gccttttcgt ctgtgatatc
      841 catcaagtca tcaccaagca taacgacaaa aggttcattt ccgacgaaag ccttggcttg
      901 caaaacagca tctccgagac cgcgtggatg agtttggcgg ataaaatgca gacgcatgtc
      961 agttgtttta tcaactagct tcaaaagatc tgttttccct ttttctttga ggttatattc
     1021 caattcgaag tttgaatcaa agtggtcctc aatagaacgt tttgatttac cagtgacaac
     1081 tagaatatct tcaatacctg atttgagagc ttcttccacg ataaactgga tagttggttt
     1141 gtctacgatt ggcaacattt ctttggcaag ggccttggtt gctggtaaaa atcgagttcc
     1201 tagtccagca gcagggatga ctgcctttct aacttttgat gtcataaaaa tcctttctat
     1261 agagggttaa gaccactcat tttctgcttt aaattcattg ttcatgatgt cataaatggc
     1321 atctttgata ttggttccgt gataaataac ttggtaaata gcctgtgtaa tgggcatata
     1381 gactccaagt tcttgggcta gttcataggc tgctcgagtc gttgaaattc cttcgattac
     1441 catgcccata ttagcttcta tatcagctag ggattctcct cgtccgagag catctccagc
     1501 tctccagtta cgagagtgga tggaagttcc cgttacgatc aaatctccca caccagataa
     1561 gccgctatag gtcaatggac tggccccgag tgctacccct aggcgggtga tttctgctaa
     1621 acctcgagcg atgatggctg ccttagcatt atcaccaaat ccaagaccat gtaaagctcc
     1681 agcaccgaca gcaataatat ttttaagagc accagcagtt tcaaccccga taacatccgt
     1741 attggtataa agtcggaagt agtgattact aaatagcttc tgaacgtatt gagctgtttg
     1801 taaatcttta gaagcagcag ttattaaagt taggtcacgc acaatggtct cttctgcatg
     1861 actaggccct gaaacaacga cgatatcact acggagatgt tcaggaattt cttcttcaag
     1921 aatggttgat aatcgtttat ggctatcagg ttctaatccc tttgatgcgt gcatgatgat
     1981 aaccttatgg tccaaggttt gtgcaacttg ctgggcaaca agtcgtgtca cttttgttgg
     2041 gacaacaaac aaaatcgcat ccacatcttt caatgtttct gctaagtcgg tgtaggcaat
     2101 gatattttcg tctagaacga catctttaaa gtagtgctta ttagtatggt gtgtattaat
     2161 ttcattgatt tgctcgggaa gatttcccca aatacgtacc tcgtgtccat tgtcatttaa
     2221 gacttgtgaa agggcggttc cccaagaacc aggccccaag acggcgacgg tttgtttttc
     2281 catgatttcc tccttgatag agttctttct cttattctat catattctag gggattttgc
     2341 acttgcatta ctggggtatg gtggaaaaat acacaaaaac cgagacgaag ggataaactc
     2401 ttagtcttta gttaaagtga gaagaaatct gatagatagt ttgattcagt atatgcttat
     2461 aatagaggta aacaactcag gaagttcttg taggttgcga gagtttggcg aaaaaattaa
     2521 aagattacgt ttggctaaaa aaatcagtcg ttcagaattt tgtggtgatg agtctgaatt
     2581 aagtatccgt caattaatta gaattgaaaa tggagaatcc agaccaatac taacaaagtt
     2641 aaaatatatt gctgaacgtt tgggggttga agattacaag ttgatgccaa gttatataga
     2701 gttggataag gaatacctag aattgaagta tttcttgatg aggactccta catacgaaga
     2761 tgaaactatc gcccaaaaga aagagagtgt ttttgataag atttttgaag agtattatga
     2821 taggctacct gaggaagaaa gatttatcat cccaaattat tcatatctgg cactaacgaa
     2881 ctacacagtt caaaaattac cagaaaagct agttgaaata ctgtccttct ggtgattttg
     2941 cttaatttct gatgttcgag acataattga ggagatttgt cttgattttt tcctaaaaaa
     3001 gggcagactc ctcccttggt ttgatgctcg atttttaaaa taaggtgact tattctgtta
     3061 ggcatgtttt cccctgccct ctagctctat atggaacagg gagtatcaga tttatctggc
     3121 agattgtatc aaataaggct tgaaactgtt ttgaataggc gtatagactt gagaatttga
     3181 gaatatgtcg tctagcagta gagacatatt ttccggcaat atgaaggaag agacgtcgaa
     3241 aacgcttgat agtcaaggac tttacttcat caccagctag ctgctttaaa aagaggtaga
     3301 gattataagc cagacagccc atcatcatac gtacttcatt cttaatcatg gtcgaactat
     3361 ctgtcttatc cccaaagaat cctgctttcc tttctttgat aaagttttca gcattccctc
     3421 gttcacgata gaattgaaac aactcctcac tatctacact gcttaaattg gtcaaatgaa
     3481 cttttttaca accataaaaa agagtactta atcgtactct tctcttttaa ggattagttg
     3541 aattaattca aatccactag ttctcctgtt tctaggtaga ctaggcgttc acaaatgtta
     3601 gcaatgtaat cagcgaagcg ttccagatgc cctatgatat aaaggtattg agttccattg
     3661 ggaattgagg tttcttggtc tttcataagt ccaatgattt cctttgataa ggcataataa
     3721 tattggtcaa tctgttcatc tttttgagca atactaatag cttttgaggc ttggtgcaaa
     3781 ggaaaggcaa ccaataaatc ggctagcatg ctgagggata atttacccat ttggtgtaac
     3841 tgttcttcgt caggggctag ttgattttct tttagttgca aaacagcttt ggcaatgcct
     3901 gccatatggt ctcccatacg ttcaaggtct gaacaagaag acatgatgct aatcacaaat
     3961 cgaaggtcag acacttgtgg ctgctgcaag gccaacaaac gggcacaggt caattcgata
     4021 gcgctttgac cttggttgat agcatgatcc ttattgataa ttagctctgc catctccttg
     4081 tctttggagg ctaaggccag taaggctttt gaagctgttt caaggacaag ttgccctagt
     4141 cctaaaaagg attgttctaa ttcatgcaat tctaagtcaa attgatttct cataggtttt
     4201 tccttcctta tccgaaccgt cctgaaatat agtcttctgt gcgctgatct tttggattgg
     4261 taaacacgtc aacggtatct ccaaattcgc aaatttctcc tgttaagaaa aaagcagttt
     4321 tatctgaaat acgtgaagct tgttgcatgt tatgggtaac aatgataatc gtataatcct
     4381 tttttagttg ctgaatgagg tcttcaattt ttaaagtgga gatagggtct aaggctgaag
     4441 tcggctcatc cattaacaga atatcaggtt ctactgctaa agctcgcgca atgcaaaggc
     4501 gttgctgctg accgccagat aaggacatgg cactcttttt aagatcatct ttgacttctt
     4561 cccaaatggc tgcccctttt aaagatttct ccactaaggc atctaattgt tttttgtctc
     4621 gaataccatg tgtccttggg ccataagcca cgttatcata gatagacatg gcaaagggat
     4681 taggctgttg aaaaaccatc cctacacgct tacgtagctg attaaggttg aatttgctac
     4741 tataaatatc ttgctcatct aagaggactt ggccttcaat atggcaagaa ggaaccaaat
     4801 cgttcatccg gttaagggtt tttagaaaag ttgatttgcc acaaccagat gggcctatca
     4861 aggcagtaat ctgtctttct ggtaattgaa tcgaaatatt ttttaaggct tgaaaatccc
     4921 cgtaaaataa gtctaggtgt ctgactgaaa atgttcccat actaggaagc tcctttcaca
     4981 agttttcgag ataataagct tgatagagta tttatcatta aaacagtaat aatcaaaatc
     5041 acgccggtag cataggcttc attgacatgt agcccctcac ttgacagcat atacatatgt
     5101 agggctagag aacggcctga agacatgaga ctacttggcg tattggtaga ggtacctaat
     5161 gtatacatga gggcagctgt ttcaccaacg atacggccaa tagctagtat cactccagct
     5221 aaaatacctg gcatggcaac tggtagaaca attctaaaaa cagtccgtaa cttacctgcc
     5281 ccaagtccat aacttgcttg acgcatgcta tcactaacag ataaaagggc ttcttctgtt
     5341 gagcgaataa tgactggcaa caccatgata actgaggtta agattcctga taacagagag
     5401 tattgaaaac ctaagaagac tacaaagaag agcatgccaa acagaccaaa aacaatggaa
     5461 ggaatcccag ataaggtatc tgaggccaat cgcatgattt taacacaaag ggaatctttt
     5521 tttgtatatt ccacaagata aaaaccagca aaaatcccta tgggcaaggc taaaagaaga
     5581 gcaccaaaga ccagaataac ggtggaaata atcgctggca taagggaaat gttctcagaa
     5641 gtataagtcc aagaaaagag ggatagactt agatgaggta agcctttgat gaggataaaa
     5701 ccaatgatta aaaagagaga gccaaaggtt aaagctgaaa aacaataaac gagaagtttt
     5761 agcaggtatt tactcataag atgattttcc tttcaagtag gcaaagtagg cattaatcaa
     5821 gagaataagg aaaaagagaa ctgctgaggt tgcaataagg gcttccctat gctgacctga
     5881 tgcgtaagcc atttccagaa caatattggt tgttaaggtt ctggttcctg aaaagagtcc
     5941 acttggaata atcggctggt tgcctgccac caaaataact gccatggttt cacctactgc
     6001 gcgaccgatt cctaaaataa ctgctgataa aataccagat ctcgcagctg gcaagatgac
     6061 actaaaaata ctccgttcat gactagctcc tagagccaag ctaccagaat aatacgtttt
     6121 gggaactgtt cggatagcag attctgacaa actgataatg gttggcaaaa tcattattcc
     6181 taatagtaac gaagcggtta ggacactcat gccatttcct aaaaagcttc taatccaagg
     6241 caccaataat tgtaggccga aaaaaccata aacaatagat ggaatggctg ccatcaagtt
     6301 gatagctgat tttaagaagc catagacggg ctttggacaa taataaacca taaacaccga
     6361 tgtcaagatg cctgttggca ccccaatcac aatcgctcct aaggtaatta ataaggaacc
     6421 aacgatcatt ggtaaaatac catagcttgc cggaatgttc gttggcgacc aatcactgcc
     6481 taataaaaaa cgggcaaagc cgtagttagc tatgaaaggt aagccattac taaaaataaa
     6541 gaaacagatt agcaaaatag ctacaacagc tactgttgca ctcatgaaaa aaattgccct
     6601 aaaaactgct tctttgaagg cttgttttgt cacatcttgt cctttctagt gaagaaagta
     6661 agggagatac gacacctccc tacttgcctt ctttatctta ttgtacgatg aaacgtctgc
     6721 atctctttag agatttatgg agcaaacatt ttatttaatc ttgtcccagg tggttaattt
     6781 gccactaaaa acgtctgcaa gttcagccat actgacttgg cttgccttat tgtcattatt
     6841 gaccacaaca gcaataccgt ctaaagcaat agcatcatgg gtgagactct taccttcttc
     6901 aggagttaat tccctagaaa ccataccaat atcagcggtt ttctccttaa cagcggtaat
     6961 acctgctgaa gacccattag aggtaatatc aatcgtaact tctggatttt cttttttata
     7021 agcttctgct aatttttcca ttaaagaaga tactgaagtg gaacctacaa cagacaactt
     7081 gcctgataag tgttggcttg tatattccgt ggtttcggtt ttagcttcaa taaatttatt
     7141 atctgtgacc acttgttgac cttgtttgga gtggataaag ctgataaaat cttgacctag
     7201 cttggaaaga ttagaagacc aaacaatgtt gaagggacgt tgaagagggt attcaccatc
     7261 taaaactgtg tctcgactag ccttgacacc atcaatctct aaagccttga cagatttcgt
     7321 taaagatccc aaggagatgt agccgatagc attagcattc ccttgaactg ctgagagaac
     7381 accttctgta ctattttgaa tcacagctgt tttggcagtg ttgtcaattt ttttatcacc
     7441 gtcttttttg agaatccctg tgatttctgt gaaggcaccc cgtgtcccag agccattttc
     7501 tcgtgaaatc acctcaatcg ttcctgaagc tgactgtttg gaagcagctg actgattgcc
     7561 acaggcaaca agcccaaatc ctgataagcc aatggctgca agagtaagca tttttttgaa
     7621 tttcataata atcaccttta tctctatgta tttttcttgt gtaggcttac tacatttata
     7681 gtctaacaag tctttgtaaa ggtttatccc tgattcatgt aaagattgtg taaagaatca
     7741 aaaaaagcca cttttgaaaa atggctgctc ctaaaaatag ctttaaaaat tattagtcct
     7801 gtgcgaaaga ttggttagga agaaaaatcg tgaagcaact gcctctgcca agctgactcg
     7861 tcaccgtgac ttggccacct aataattgac tgagttcttt gacaatggca aggccaagac
     7921 cagtgccacc agtttgtctg cttcgacctt tattaactcg gtaaaaacgt tcaaaaatac
     7981 gatcctgctc taattgacta ataccaatcc ctgtatctga tacagaaatc ttaatgcctt
     8041 cgttcacctt ttgggtcttg acctcaattt ttcccccttg ttcagtgtaa cggatggcat
     8101 tggataaaag attgagtaag atttgggaaa gtaattgact atctgatacg agggtgacat
     8161 catctggcac ctgcaccttt agctgtaaat ccttcttctt gagctgaggt tgcaagcttt
     8221 gagtcaaatc ctgtacaaat tctgccaaag aaagggtcgt ccattgtata ggcatttgtt
     8281 gagccttaga taaggtaaga agatgctcaa caatatgctc aagacgcaaa ctttctttgt
     8341 aaataatgtc tagaaagtca tccttgagcg cttcttcttc agctgacatc cccttaatgg
     8401 tttcagcaaa gcccttaatc gaagtaactg gtgtcctcaa ttcatgggag gcatttgaga
     8461 caaaggctaa atttaacttt tcataagttc taatcgttgt taaatcatat agcaagacga
     8521 gcacagcttc cacagattgg gtggggctaa aaacgggaac tgctgtcact tctaaaatca
     8581 agtcaccctc atgaaaccca cttacttctt gttttaacct tgttttttga tcaaaggctt
     8641 ggtgaactaa attccgaata tccatccgtt tgaggtcatc aagtgaactt atgtcgccgt
     8701 ccacatcggg aaaataatga ggcagagagc gactggataa taacatctga ccttgagcgg
     8761 aaactaaaaa cgtccccatg gttaggtgcg acagaagaac ctccattgtt tcggctagat
     8821 ccttgtattg ctgatcctgt tgggagactt tggtttttag gccagacaca tactgagcca
     8881 aagactttaa gtcttcttgc cctttttcta aaaagtattc actactggtc aagagaggtt
     8941 ggtgcaaggt ctcaaaagca acttcccatt tccaaaggca aaagagccag tagccaccta
     9001 gtcccaaaga aagggctaga agaaagagac cgatgccttt actgatccaa gttaatgcca
     9061 tccctgcaat cagaatgagg ctaacactta gattgactag ccaaaattga aggtagcgtt
     9121 tcatctataa ctccttgaac ttataaccat aaccccgaat ggttcgaata aattgagggg
     9181 ctttaggatt gtcttcaatt ttttccctca acttaccaat atgaacgtcc accaaacgtg
     9241 tttcctgccc aaagtcatac ccccagatac gttccaaaag acgctctcta gtcagtgtca
     9301 tgttgggatg tttcataaga tagagcaaga gttcaaattc ttttggggtc aaactcagta
     9361 acttattcgc cttgtagact tcatgacgct cagggtatac tttcaaggtc ccaaatagcc
     9421 aagaatcgtc agcgatatta tctgaatcat ctccttcttg ttctccttta gttcgcctga
     9481 ggacagcctt gacacgcgcc agcaattctc tagggctaaa aggcttggtc aggtagtcat
     9541 cagcccctaa ttccaaggcc aaaaccttat caaattcatc acttttcgca gaaaccatca
     9601 taattggagt tttgacgcct ttggctctca gccgcttaca aacttccatg ccatctaatt
     9661 gtggtaacat gatatcaagc aagataaaat caaagggttc tgtttctgcc aaagctaagg
     9721 ccttccgtcc atttgtcacc aattgagtag aaaagccttc cttacttaaa tggtagtcaa
     9781 gcaatttcag aatgtgttct tcatcatcca ctaataagac ttgttttgtc atctattatc
     9841 tcctattggt aacattataa cacaattatc agaaatccta acattgctaa atcagattaa
     9901 atttgcctat caagactagt atctggtcaa acgctcaatc atctccttgt gctctggata
     9961 ggtcgccagt agatctaccc tttcaaataa ttcaaaatcc tcaaattcaa aaccaggagc
    10021 aacaagacaa gaaaccagag catcatcctt atcaactgtt gatccccaaa tagtgccctt
    10081 aggaacacag tagtgaagtt gttgcccttt ggatatgtcc aggcctaaag tgactgcttc
    10141 gtagtgacca tctgctgtaa tcatgtgaac agtaagtggg gatcctgcat gaaaatacca
    10201 gatttcatct gctgtcaatc ggtgaaaatg tgaaggattc gtttcttcta ataagaaata
    10261 aatactggta taaagcgccc ttcccttacc agcaaggttt atagtgtctg aagctttttt
    10321 tgtttgtcta aaatagccac cttcaatatg gggagctaac tctagagttc ttatcaagtc
    10381 ttctttatcc gtcggagcca atgggttgaa gtaactcttg ttcaaagtgg ttttacgatt
    10441 tcaagaactc ctctcagttc tgaggacacg gtaatgattg atgcgacgga agtacaaatc
    10501 aatcgcccta aaaaaagaat tagcgaatga ttctggtaaa aaaaatgcca cgctatgaag
    10561 gctcaagcga ttgtcacaag tcaagggaga attgtttctt tggatatcgc tgtgaactat
    10621 tgtcatgata tgaagttgtt caaaatgagt cgcagaaata tcggacaagc tggtaaaatc
    10681 ttggctgaca gtggttatca agggctcatg aagatatatc ctcaagcaca aactccacgt
    10741 aaatccagca aactcaagcc actaacagtt gaagataaag cctataacca tgcgctatcc
    10801 aaggagagaa gcaaggttga gaacatcttt gccaaagtaa aaacgtttaa aatgatttca
    10861 acaacctatc gaaatcatcg taaacacttc ggattacgaa tgaatttgat tgctggcatt
    10921 atcaatcatg aactaggatt ctagttttgc aggaagtcta ttatttggtt aggtgaatta
    10981 gtgaagcgtt taggcaagtg tctctggtta cgacgtcatg gactctaaat cgattatatt
    11041 taggggtcat gactagtgaa gcagttagct agttcgcata taagcggcta gcgtctaaca
    11101 attaggaact ttagttccaa taactttaag attacgacgt tttaggacat aaatcgatca
    11161 tatttatgtc ctaaaactag tgaagcgcct agccaaagtc cgaataggat ttggcgttag
    11221 ttacttagat tgctttgcaa tcaagtaact ttggcgattt acatcttctc tggcgcttct
    11281 actccaagca agcgaagggc ttctttgaga acgactgcgg ttgcgtagct gagggctaga
    11341 cggctgtcgc gttctgggct ttcatccaag atacgtgtat gtgcatagta tttgttaaag
    11401 gattgagcca ggctaattgc aaatttagca atgatagaag gttcaaagtt atctgccgca
    11461 cggttgataa tacgtgggaa gtcttgaatg agtttaatga tttcccagct ttcagtatca
    11521 ttcaagctat agttgccagc tgtttctggt ttgaaatcgg ctttgcgtaa gatagattgg
    11581 atacgagcgt aggcatattg aacgtaaggt ccagtttcac cctcgaagga taccatagcc
    11641 tctaggtcga agtcgtatcc atttgtacgg tcggttttga ggtcatagaa tttaatggct
    11701 ccaatcccaa cagcatgtgc tacttggtct ttgttttcta gttcaggatt tttagcctcg
    11761 atttggacct tggcacggct aacagcctct gcaacagtag gctctagcaa gatgacattc
    11821 cctttacgag tagagagttt cttcccttct tttgtaacca aaccaaaagg aacgtgagta
    11881 atgtcgtcac tccagtcgta gcccatctct tgcaagacag ctttgagctg tttaaagtgg
    11941 gcagattgtt cttgaccaac gacatagata gatttagcaa attggtattc gtttttacgg
    12001 tagagggctg cagccaagtc acgtgtgata tagagagttg caccatcaga cttcttgatg
    12061 agggctggat gttcaattcc atatttctca agattcacaa cttgggcacc ttctgattca
    12121 agaagtagtc ctttttcaga aagaatgtct acaactgcat ccatcttatc attgtagaag
    12181 gcttctccgt tatagctgtc aaattcaacc ttcaattcat tgtaaaggcg gttaaattcc
    12241 actaaacttt catcgcggaa ccattgccaa agagcgagag cttcctcatc tccattttca
    12301 agtttacgga accattcgcg cgcttcttca tccaagctag ggtcattttc agcttcagcg
    12361 ttgatgcgga catagagttt aaggagttca tcgattggat gagcttttac agcttcttcg
    12421 tcgccccatt ttttgtaggc aacaatcaac atcccaaatt gtttacccca gtctcccaaa
    12481 tggttgacct tgaccgtttg ataaccgatt ttttggaaaa tatgtgacaa gctatctccg
    12541 ataacagttg aacgcaggtg gccaatagaa aatggtttag cgatattcgg actagacatg
    12601 tcgataacaa cattttcttg tttaccaata ttttggtcag catagtgttc tttttcagtg
    12661 gtaacagctt gcaatacttg agcagaaatg gcagatttat caaggaaaaa gttaacgtaa
    12721 ggtcctgttg cgacaacttt ttcaaaggct tggctgttca ttttttcagc cagttcagcc
    12781 gcaatcattt gtggtgcttt acgttcgact tttgcaagag aaaaagcagg gaaagcaatg
    12841 tctcccattt ctgagttttt aggggtttcc agtaacttta aaatagcctc ttggtccagg
    12901 ctatcaatga tgctagataa ttcgctagca atcaattctt ttgtattcat taagagctcc
    12961 tttttggact tttctactat tttatcacaa ttttaaagaa agaagaaaaa atttttgaaa
    13021 tctcctgttt ttttggtata atatggttat aaatatagtt ataaatatgc acgcaagagg
    13081 attttatgag aaaaagagat cgtcatcagt taataaaaaa aatgattact gaggagaaat
    13141 taagtacaca aaaagaaatt caagatcggt tggaggcgca caatgtttgt gtgacgcaga
    13201 caaccttgtc tcgtgatttg cgcgaaatcg gcttgaccaa ggtcaagaaa aatgatatgg
    13261 tgtattatgt actagtaaat gagacagaaa agattgattt ggtggaattt ttgtctcatc
    13321 atttagaagg tgttgcaaga gcagagttta ccttggtgct tcataccaaa ttgggagaag
    13381 cctctgtttt ggcaaatatt gtagatgtaa acaaggatga atggatttta ggaacagttg
    13441 ctggtgccaa taccttattg gttatttgtc gagatcagca cgttgccaaa ctcatggaag
    13501 atcgtttgct agatttgatg aaagataagt aaggtcttgg gagttgctct caagacttat
    13561 ttttgaaaag gagagacaga aaatggcgat agaaaagtta tcacccggca tgcaacagta
    13621 tgtggatatt aaaaagcaat atccagatgc ttttttgctc tttcggatgg gtgattttta
    13681 tgaattattt tatgaggatg cggtcaatgc tgcgcagatt ctggaaattt ccttaacgag
    13741 tcgcaacaag aatgccgaca atccgatccc tatggcgggt gttccctatc attctgccca
    13801 acagtatatc gatgtcttga ttgagcaggg ttataaggtg gctatcgcag agcagatgga
    13861 agatcctaaa caagcagttg gggttgttaa acgagaggtt gttcaggtca ttacgccagg
    13921 gacagtggtc gatagcagta agccggacag tcagaataat tttttggttt ccatagaccg
    13981 cgaaggcaat caatttggcc tagcttatat ggatttggtg acgggtgact tttatgtgac
    14041 aggtcttttg gatttcacgc tggtttgtgg ggaaatccgt aacctcaagg ctcgagaagt
    14101 ggtgttgggt tatgacttgt ctgaggaaga agaacaaatc ctcagccgcc agatgaatct
    14161 ggtactctct tatgaaaaag aaagctttga agaccttcat ttattggatt tgcgattggc
    14221 aacggtggag caaacggcat ctagtaagct gctccagtat gttcatcgga ctcagatgag
    14281 ggaattgaac cacctcaaac ctgttatccg ctacgaaatt aaggatttct tgcagatgga
    14341 ttatgcgacc aaggctagtc tggatttggt tgagaatgct cgctcaggta agaaacaagg
    14401 cagtcttttc tggcttttgg atgaaaccaa aacggctatg gggatgcgtc tcttgcgttc
    14461 ttggattcat cgccccttga ttgataagga acgaatcgtc caacgtcaag aagtagtgca
    14521 ggtctttctc gaccatttct ttgagcgtag tgacttgaca gacagtctca agggtgttta
    14581 tgacattgag cgcttggcta gtcgtgtttc ttttggcaaa accaatccaa aggatctctt
    14641 gcagttggcg actaccttgt ctagtgtgcc acggattcgt gcgattttag aagggatgga
    14701 gcaacctact ctagcctatc tcatcgcaca actggatgca atccctgagt tggagagttt
    14761 gattagcgca gcgattgctc ctgaagctcc tcatgtgatt acagatgggg gaattatccg
    14821 gactggattt gatgagactt tagacaagta tcgttgcgtt ctcagagaag ggactagctg
    14881 gattgctgag attgaggcta aggagcgaga aaactctggt atcagcacgc tcaagattga
    14941 ctacaataaa aaggatggct actattttca tgtgaccaat tcgcaactag gaaatgtgcc
    15001 agctcacttt ttccgcaagg cgacgctgaa aaactcagaa cgctttggaa ccgaagaatt
    15061 agcccgtatc gagggagata tgcttgaggc gcgtgagaag tcagccaacc tcgaatacga
    15121 aatatttatg cgcattcgtg aagaggtcgg caagtacatc cagcgtttac aagctctagc
    15181 ccaaggaatt gcgacggttg atgtcttaca gagtctggcg gttgtggctg aaacccagca
    15241 tttgattcga cctgagtttg gtgacgattc acaaattgat atccggaaag ggcgccatgc
    15301 tgtcgttgaa aaggttatgg gggctcagac ctatattcca aatacgattc agatggcaga
    15361 agataccagt attcaactgg ttacagggcc aaacatgagt gggaagtcta cctatatgcg
    15421 tcagttagcc atgacggcgg ttatggccca gctgggttcc tatgttcctg ctgaaagcgc
    15481 ccatttaccg atttttgatg cgatttttac ccgtatcgga gcagcagatg acttggtttc
    15541 gggtcagtca acctttatgg tggagatgat ggaggccaat aatgccattt cgcatgcgac
    15601 caagaactct ctcattctct ttgatgaatt gggacgtgga actgcaactt atgacgggat
    15661 ggctcttgct cagtccatca tcgaatatat ccatgagcac atcggagcta agaccctctt
    15721 tgcgacccac taccatgagt tgactagtct ggagtctagt ttacaacact tggtcaatgt
    15781 ccacgtggca actttggagc aggatgggca ggtcaccttc cttcacaaga ttgaaccggg
    15841 accagctgat aaatcctacg gtatccatgt tgccaagatt gctggcttgc cagcagacct
    15901 tttagcaagg gcggataaga ttttgactca gctagagaat caaggaacag agagtcctcc
    15961 tcccatgaga caaactagtg ctgtcactga acagatttca ctctttgata gggcagaaga
    16021 gcatcctatc ctagcagaat tagctaaact ggatgtgtat aatatgacac ctatgcaggt
    16081 tatgaatgtc ttagtagagt taaaacagaa actataaaac caagactcac tagttaatct
    16141 agctgtatca aggagacttc tttgacaatt ctccactttt ttgctagaat aacatcacac
    16201 aaacagaatg aaaaggagct gacgcattgt cgctcccttt tgtctatttt ttaaggagaa
    16261 agtatgctga ttcagaaaat aaaaacctac aagtggcagg ccctggcttc gctcctgatg
    16321 acaggcttga tggttgctag ttcacttctg caaccgcgtt atctgcagga agtcttaggc
    16381 gccctcctta ctgggaaata tgaagctatt tatagtatcg gggcttggtt gattggtgtg
    16441 gccgtagtcg gtctagttgc tggtggactc aatgttgtcc tcgcagccta tattgcccaa
    16501 ggagtttcat ccgaccttcg ggaggatgcc ttccgtaaaa ttcaaacctt ttcttatgct
    16561 gatattgaac aatttaatgc gggaaatcta gtcgttcgaa tgacaaatga tatcaaccag
    16621 attcagaacg ttgtcatgat gaccttccaa attcttttca gacttcccct cttgttcatc
    16681 ggttcgttta tcctagcggt tcaaacctta ccttctctgt ggtgggtgat tgttctcatg
    16741 gtagtcttga tttttggttt gactgctgtc atgatgggaa tgatggggcc tcgttttgcc
    16801 aagtttcaaa cccttcttga gcgcatcaat gccattgcca aggaaaattt acgtggcgtt
    16861 cgtgtggtca agtcctttgt ccaagaaaaa gagcaatttg ctaagtttac agaggtctca
    16921 gacgagcttc ttggtcaaaa cctttacatt ggttatgcct tttcagtagt ggaacccttt
    16981 atgatgttgg ttggttacgg ggcggtcttc ctctctattt ggctggtcgc gggaatggtt
    17041 cagtcggatc cgtctgttgt tggttccatc gcttcttttg ttaattacct aagccagatt
    17101 atctttacca ttgttatggt tggatttttg ggaaattctg tcagccgtgc catgatttcc
    17161 atgcgtcgta ttcgagaaat tcttgacgca gagccagcta tgaccttcaa ggatatccca
    17221 gatgaagagt tggttggaag tcttagcttt gaaaatgtga cctttaccta tccaatggac
    17281 aaggaaccga tgctgaaaga tgtgagcttt actattgaac ctggtcaaat ggttggtgta
    17341 gttggagcga ctggtgcagg aaagtcaacc ttggctcaat tgattccacg tctctttgat
    17401 ccacaggacg gggccattaa aatcggtggc aaggatattc gagaagtgag tgaaggaacc
    17461 ctgcgtaaaa cagtttccat cgttctccaa cgtgccattc tttttagtgg aacgattgca
    17521 gataacttga gacaggggaa ggggaatgct actctatttg aaatggagcg cgcagccaat
    17581 attgcccagg ctagtgaatt cattcatcgt atggagaaaa cctttgaaag tccagttgaa
    17641 gaacggggaa ccaatttctc tggtggacaa aaacaaagga tgtcgattgc gcgtgggatt
    17701 gtcagcaatc cacgtattct gatttttgat gattcgacct cagccttgga tgccaaatca
    17761 gagcgcttgg tgcaagaagc tttgaataag gacttgaagg ggacgacaac cattattatt
    17821 gctcaaaaaa ttagctcggt tgtccatgca gacaagatct tggttctaaa tcaaggacga
    17881 ttgattggtc aaggtacgca tgcagacttg gttgccaaca atgccgttta ccgtgaaatc
    17941 tatgaaacac agaaatgaaa gacaaactat aagaaaagtc aatagtttta tctaaactat
    18001 ttcttatttc aatttgatga tttggcgatg attttagagc acagcaaaaa gcccttgaaa
    18061 aagtccattt tttcaaaggt aatcctgtgt taatttcaga aattacatca ctttttgttc
    18121 gtcaaatggc agctcttttt ttaggatata aaacagggtt cggataagtt tttttgcaag
    18181 gtggatgatg gctacattgt aatgttttcc ttgttctaat ttagtcttaa gataggcctt
    18241 aaaagcaggc gaaaagcgag ggcatgcttt ggcagcttgt atgagtacct accgcagatg
    18301 aggggaactc cgtttgacca ttcttcctgc taaatcaatc tgatctgact gataaataga
    18361 agaatccagt ccagcgaaag cttgtaattg agcaggatta tcaaaggcat gaatatttcg
    18421 aatctcagct aaaatgaccg cccctaaacg atccccaatc ccagtaaccg tcgtgatgac
    18481 cgagttgaac tcagccatca agtcattgac acatgtttcc gccttgtcaa tgagcctctt
    18541 gtaatgtttg atgttttcat tacacgagat aaaacgtcta tgcgttatca aactcattac
    18601 caattaaaac aaaaagctgt ggttagatcc tttcggaaat tgtcaagcga ttggaggaaa
    18661 tgaactaatc cacagcggct tattccaagt ataccacttg ggctttggca gtagctaact
    18721 gcgctaaata taatataagg aggagtaaaa tgaagacagt tcaatttttt tggcattatt
    18781 ttaaggtcta caagttctca tttgtagttg tcatcctgat gattgttctg gcgacttttg
    18841 cctaagccct ctttccagtc ttttctggac aagcggtgac gcagctagcc aatttagttc
    18901 aagcttatca aaatggcaat ccagaacttg tatggcaaag cctatcagga atcatggtca
    18961 atcttggcct gctggttttg gttctattta tctctagtgt aatatacatg tgtctcatga
    19021 cgcgcgtgat tgcagaatcg accaacgaga tgcgcaaagg cctctttggt aagcttgctc
    19081 agttgacggt ttctttcttt gaccgtcgac aagatggcga tatcctgtct cattttacca
    19141 gtgatttgga taatatcctc caagccttta acgaaagctt gattcaggtc atgagcaata
    19201 ttgttttata cattggtctg attcttgtca tgttttcgag aaatgtgacg ctggctctca
    19261 tcaccattgc cagcacccca ttggctttcc ttatgctgat tttcatcgtg aaaatggcac
    19321 gcaaatacac caacctccag cagaaagagg tagggaagct caacgcctat atggatgaga
    19381 gcatctcagg ccaaaaagcc gtgattgtgc aaggaattca agaggatatg atggcaggat
    19441 ttcttgaaca aaatgagcgc gtgcgcaagg caacctttaa aggaagaatg ttctcaggaa
    19501 ttcttttccc tgtcatgaat gggatgagcc tgattaatac agccatcgtc atctttgctg
    19561 gttcggctgt acttttgaat gataagtcta ttgaaacaag tacagcccta ggtttgattg
    19621 ttatgtttgc acaattttca cagcagtact accagcctat tatccaagtt gcagcgagtt
    19681 ggggaagcct tcagttggcc tttactggag ctgaacgaat tcaggaaatg tttgatgcag
    19741 aggaggaaat ccgacctgaa aaggctccaa ccttcactaa gttgcaagaa agtgttgaaa
    19801 tcagtcatat cgttttttca tacttgcctg ataaacctat tttgaaagat gtcagcattt
    19861 ctgcccctaa aggccagatg acagcagttg ttgggccgac aggttcagga aaaacgacta
    19921 ttatgaacct catcaatcgc ttttatgatg ttgatgctgg tggtatttat tttgatggta
    19981 aagacattcg tggctatgac ttagatagtc ttagaagcaa ggtgggaatt gtattgcaag
    20041 attcggtctt gtttagcgga acgattagag acaatatccg atttggtgtg ccagatgcta
    20101 gtcaggaaat ggttgaggta gcagcaaaag caacccacat tcacgactat atcgaaagtt
    20161 tgcctgataa gtacgatact cttattgatg atgaccagag catcttttca acagggcaga
    20221 agcaattgat ttcaatcgct cgaaccctga tgacagatcc agaagttctc attctcgatg
    20281 aagcaacttc aaacgtagat acggtgacag aaagcaagat tcagcatgcc atggaggtgg
    20341 ttgtagcagg tagaactagt ttcgtcattg cccaccgctt gaaaaccatt ctcaatgcag
    20401 atcagattat tgtccttaaa gatggagaag tcattgaacg tggtaaccac catgaacttt
    20461 tgaagctagg tggcttttat tcagaactct atcacaatca atttgttttc gaataagaaa
    20521 gaagttgtcc tatgtgggca gctttttctt gtccataaaa aatgtttatc acagccttaa
    20581 aaaaaacata ttagacgaaa gtcattttga gtgatatgat aggactatcg ttagcattcg
    20641 aaaggagagg catcatggct agaacggttg taggagttgc tgcaaatcta tgtcccgtag
    20701 acgcagaagg caaaatcatt cattcatctg tatcttgtag attcgcagag atcattcgtc
    20761 aagtcggtgg tctcccttta gtcattcctg ttggtgatga gtcagttgta cgtgattatg
    20821 tggaaatgat tgacaaactc attttgacag gaggccaaaa tgttcatcct cagttttatg
    20881 gagagaaaaa gaccgtcgag agcgatgatt acaatctggt ccgtgacgaa tttgaattgg
    20941 cactcttgaa ggaagcgctt cgtcagaata aaccaattat ggcaatctgt cgcggtgtcc
    21001 aacttgtcaa tgttgccttt ggtggaaccc tcaatcaaga aatcgaaggt cactggcaag
    21061 gactaccttt cgggacatct cactctattg agacagtaga aggaagcgtg gtggctaagc
    21121 tatttgggaa agaaagtcag gtcaactcag tccatcgtca aagcattaaa gatttggcac
    21181 ctaatttccg tgtaactgct attgattcaa gagaccaaac tattgaagcg attgagtcta
    21241 tcgacgagca ccgcattatc ggtttacagt ggcatccaga gtttctggtt aatgaagaag
    21301 atggcaattt agaattattt gagtatttat tgaatgaact gtaacgattg gaacatctgg
    21361 tcgttctttc ttttattttt tcaaaatttt tagaatgtgg cattttacgc aaacgtttga
    21421 attctgataa aaattggaga aattcgacaa aaaaacttga aaaaaacgaa ggtaagcgtt
    21481 atgatagaaa agaagaaata ttggaggaag aacatgtcac atattaaatt tgattattca
    21541 aaagttttag acaaatttgt tgcaccacat gaagtggaat acatgcaatc acaagtaaca
    21601 gcagcagatg aattaatccg taaaggaact ggtgctggta gcgacttctt gggatggttg
    21661 gaccttcctg aaaaatacga ccgcgaagaa tttgaccgca tcttgaaagc tgctgagcaa
    21721 atcaagtcag acagcgatgt tttggttgtg atcggtattg gtggatctta ccttggtgca
    21781 aaagcagcaa tcgacttctt gaatcaccat tttgctaact tgcaaacaaa agaagaacgc
    21841 aaagctccac aaatccttta cgcaggaaac tcaatctcat ctacttacct tgctgacttg
    21901 gtagagtatg ttgcagacaa agacttctca gtaaacgtaa tttctaaatc aggtacaaca
    21961 actgaaccag cgattgcttt ccgtgtcttt aaagaactct tggttaagaa atacggtcaa
    22021 gaagaagcta acaaacgtat ctatgcaaca actgaccgcc aaaagggtgc tgttaaggtt
    22081 gaagcagacg ctaacggttg gggaacattt gttgttccag atgatatcgg tggacgcttc
    22141 tcagtattga cagccgttgg tttgctttca atcgcagcat caggagctga cataaaagct
    22201 cttatggaag gtgcgaatgc agctcgcaaa gactacactt cagacaaaat ctctgaaaac
    22261 gaagcttacc aatacgcagc tgttcgtaac atcctttatc gtaaaggcta tgcaactgag
    22321 atcttggtaa actatgagcc atcacttcaa tacttctcag aatggtggaa acaattggct
    22381 ggtgaatcag aaggaaaaga ccaaaaaggt atctacccaa cttcagccaa cttctcaact
    22441 gacttgcact cacttggtca atttatccaa gaaggaactc gtatcatgtt tgaaacagtt
    22501 gtccgtgttg acaaacctcg taaaaacgtg cttattccta ctttggaaga agaccttgac
    22561 ggacttggtt accttcaagg aaaagacgtt gactttgtaa acaaaaaagc aactgacggt
    22621 gttcttcttg cccacacaga tggtgatgta ccaaacatgt atgtgactct tccagagcaa
    22681 gacgctttca ctcttggtta cactatctac ttcttcgaat tggcaattgc cctttcaggt
    22741 tacttgaatg ctatcaaccc atttgaccaa ccaggtgttg aagcttataa acgtaacatg
    22801 tttgcccttc ttggaaaacc aggatttgaa gaattgagca aagaacttaa cgcacgtcta
    22861 taatagaaga aaagagtggt ttgcccactc tttttactct ctttatccat agaaattgga
    22921 ctcagccaag acttgtgata taatatagaa agcaaaaagg cagacgccta gataatagga
    22981 gaaactatgt caaaagatat ccgcgtacgt tacgcaccaa gtccaacagg actactacac
    23041 atcggaaatg ctcgtacagc attgtttaat tacttgtatg cgcgccatca tggtggaaca
    23101 tttctcatcc gtatcgaaga tactgaccgt aaacgccatg tcgaggatgg tgaacgttca
    23161 caacttgaaa accttcgctg gttaggcatg gattgggatg aaagtccaga atcacatgag
    23221 aattatcgcc agtctgagcg tttggacttg tatcaaaaat atattgacca actattagct
    23281 gaaggaaaag cctataaatc ttacgttaca gaagaagagt tggcagctga acgcgaacgc
    23341 caagaagtag ctggcgaaac accacgctac atcaatgaat accttggtat gagtgaagaa
    23401 gaaaaagcag cttacatcgc agaacgtgaa gcagcaggga tcatcccaac tgttcgtttg
    23461 gctgtcaatg agtcaggtat ctacaagtgg catgatatgg tcaaaggcga tatcgaattt
    23521 gaaggtggca atatcggtgg tgactgggtt atccaaaaga aagacggtta cccaacttac
    23581 aactttgccg ttgttatcga tgaccacgat atgcaaatct ctcatgttat ccgtggagat
    23641 gaccatattg ctaatacacc aaaacagctt atggtctatg aagctcttgg ttgggaagct
    23701 ccagagttcg gtcacatgac cttgattatc aactctgaaa ctggtaagaa gttatctaaa
    23761 cgtgatacta atacacttca atttatcgaa gattaccgaa agaaaggtta ccttccagaa
    23821 gcagtcttta actttattgc tcttcttggt tggaacccag gtggtgaaga tgagattttc
    23881 tctcgtgaag aattcattaa actttttgat gaaaaccgcc tcagcaagtc accagcagcc
    23941 tttgatcaga aaaaactaga ctggatgagc aatgattata tcaagaatgc agaccttgaa
    24001 accatctttg aaatggcaaa accattctta gaggaagcag gccgtttgac tgacaaggct
    24061 gaaaaattag ttgagctcta taaaccacaa atgaaatcag tagatgagat tatcccattg
    24121 acagatcttt tcttctcaga tttcccagaa ttgacagaag cagagcgcga agtcatgacg
    24181 ggtgaaacag ttccaacagt tcttgaagca ttcaaagcaa aacttgaagc gatgacagat
    24241 gatgaatttg tgacagaaaa tatcttccca caaattaaag cagtccaaaa agaaacaggt
    24301 attaaaggga aaaatctttt catgcctatt cgtatcgcag tttcaggcga aatgcatggg
    24361 ccagaattac cagatacaat tttcttgctt ggacgtgaaa aatcaattca gcatatcgaa
    24421 aacatgctaa aagaaatctc taaataaaga ggatacaata atggacatct gggaaaagat
    24481 gtacgaagaa gcacagaaac tatacaatcc acatgaagta tcagactttg tttatgccaa
    24541 tcatgttgta gctgcagtag aagcaggaga tggacaaata tttacaggat tctgtatgga
    24601 gggaacctgt ggtgttttcc atctctgcgc agaacgggcg gccctcttca atatgtacca
    24661 attttcagga caaactaagg ttaagaaagt attagccttt cgagacaaac caccttatgg
    24721 tggaagttca gccatgcctt gcggtgcttg tagagaattc cttttagagc ttaatgctga
    24781 aaataaagat gcagaattca tgatggacta taatataaga aaaacagtta aagtcgcaga
    24841 actaatccct tattggtggg gaaaagaacg tgcttctaag tttaatgagc gatagaagag
    24901 tcagtataat tctggctttt tttactataa gttgaaaaaa taaaaaaaaa ttagaaaaag
    24961 tagttgacaa agtttgaaaa g
//
LOCUS       DAVKNL010000028        22780 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVKNL010000028 DAVKNL010000000
VERSION     DAVKNL010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22780
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.028"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(722..1945)
                     /locus_tag="VW411_001149"
     CDS             complement(722..1945)
                     /locus_tag="VW411_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU9793880.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPYVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTAIEYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNNPIWESFNLLNVIKRFNFPFIVKNNIDCMAIGQYLFNPHNTPDNFIFLHAGLGIY
                     TSFFTKEKIGASKNPYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFK
                     NSQLTVLKSLVKTEKDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPAD
                     KIYINSQLLNYQPFTHEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVA
                     FFIEHSNVLQDIISP"
     gene            2113..3435
                     /locus_tag="VW411_001150"
     CDS             2113..3435
                     /locus_tag="VW411_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9793881.1"
                     /translation="MNNILAFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEAFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRMFIQRGITIKMPSGVPDVVSKSFSALLSGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTTENQEA
                     VAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPFIATPTINALITYVSMAVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
     gene            3437..5323
                     /locus_tag="VW411_001151"
     CDS             3437..5323
                     /locus_tag="VW411_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HEU9793882.1"
                     /translation="MVIRVFDQQKNTYSSFALEELSYYMNRVFKTNIELVEEKEADIF
                     VGLVNKEDRKDHVLISLDKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRRHDFVP
                     ELRFEDFLDKQLSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEQFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEKKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRILNQLDRALTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYMRNKIILP
                     NSLEENLSYLFEWQKAFKGDSFVYDYPLGRAHYGDLGYMKISQTIYRDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGEMLWKKTRSYEELIEEYFSALYGENWQSVVEYLEKL
                     SIYSSCDYFNAIGSRQSDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            5491..5835
                     /locus_tag="VW411_001152"
     CDS             5491..5835
                     /locus_tag="VW411_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9793883.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEAYGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            5845..7257
                     /gene="lacG"
                     /locus_tag="VW411_001153"
     CDS             5845..7257
                     /gene="lacG"
                     /locus_tag="VW411_001153"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEU9793884.1"
                     /translation="MENLQVKALPKEFLLGTATAAYQVEGATRVDGKGINMWDVYLQE
                     NSPFLPDPASDFYYRYEEDIALAAEHGLQALRLSISWVRIFPDIDGDANVLAVHYYHR
                     VFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKVGC
                     IHALKPGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSEDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAPLNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSALWVKGLKRN"
     gene            7326..9005
                     /locus_tag="VW411_001154"
     CDS             7326..9005
                     /locus_tag="VW411_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9793885.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIVWMRKVYMLTMGVLGIIVSGTVGKSLVGNVNRKMPHGKV
                     INDISAMLAAICSYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSAFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGAESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWTFPGPLGLLIGTNF
                     QLISFVFLSLILVVDILIYLPFCRAYDRQLLVKEDIASSNDIILEEDTSEIIPGEIDE
                     IKSKELKVLVLCAGSGTSAQLANAINEGANLTEVRVIANSGAYGTHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKSPSKALQFVLEHYQAV"
     gene            complement(9131..10570)
                     /locus_tag="VW411_001155"
     CDS             complement(9131..10570)
                     /locus_tag="VW411_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU9793886.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(10574..11923)
                     /gene="trkA"
                     /locus_tag="VW411_001156"
     CDS             complement(10574..11923)
                     /gene="trkA"
                     /locus_tag="VW411_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEU9793887.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            12111..12959
                     /locus_tag="VW411_001157"
     CDS             12111..12959
                     /locus_tag="VW411_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEU9793888.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            12974..13522
                     /locus_tag="VW411_001158"
     CDS             12974..13522
                     /locus_tag="VW411_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEU9793889.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLVGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            13529..13786
                     /locus_tag="VW411_001159"
     CDS             13529..13786
                     /locus_tag="VW411_001159"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793890.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEIVDRTDEVSSKHGFEVVDETDEVS
                     NHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            13888..15570
                     /locus_tag="VW411_001160"
     CDS             13888..15570
                     /locus_tag="VW411_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9793891.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQVAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLANLDNLSIS
                     KGQVQLQNELAKETPALQSLFRLEEVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            15555..16385
                     /locus_tag="VW411_001161"
     CDS             15555..16385
                     /locus_tag="VW411_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9793892.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATIFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            16578..17081
                     /locus_tag="VW411_001162"
     CDS             16578..17081
                     /locus_tag="VW411_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEU9793893.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     regulatory      17135..17369
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            17534..18097
                     /locus_tag="VW411_001163"
     CDS             17534..18097
                     /locus_tag="VW411_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9793894.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTIGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     gene            18111..18737
                     /locus_tag="VW411_001164"
     CDS             18111..18737
                     /locus_tag="VW411_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU9793895.1"
                     /translation="MKGSFALLLFVILGYMVKFYPEMLVNFDQSIQTAIRGDLPDYLT
                     ILFRALTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDQVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            18765..19154
                     /locus_tag="VW411_001165"
     CDS             18765..19154
                     /locus_tag="VW411_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEU9793896.1"
                     /translation="MKVNIADLHLTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            19159..19608
                     /locus_tag="VW411_001166"
     CDS             19159..19608
                     /locus_tag="VW411_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEU9793897.1"
                     /translation="MCFVPYYKVNHCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKKIYTYNHASRRCFKSLGFVENGATEKGMSFILELV"
     gene            19736..20323
                     /locus_tag="VW411_001167"
     CDS             19736..20323
                     /locus_tag="VW411_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEU9793898.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            20713..22320
                     /locus_tag="VW411_001168"
     CDS             20713..22320
                     /locus_tag="VW411_001168"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEU9793899.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDAEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
BASE COUNT         6813 a         3816 c         4984 g         7167 t
ORIGIN      
        1 tatacaacaa ccatttacag gtgtaaagtt gagcagtgtc tttagtaaat ttagggattt
       61 gttttgtcat agttttgata cctgtattcg ttgtttcatg agcatttgac gtttcttttc
      121 atgtaggcat ttcaacaatt agaatgatat ttttccgagg atgaacttat atattgtgac
      181 tttttagagt tatgatgcct cttgatttga ctatcgctat caatttcatc cacttttaca
      241 gtttcatggt tttatgaacc gaaacatcta ggtacaggtt ttagcatcaa ttgattgttt
      301 ttgagccata tgaagttgaa acgtggagat gcttttcttg tgataacgcc ctattcaaag
      361 aggaataacc gtttttggag agagttcagt actatgtggc gctaaatact atatgtaaaa
      421 catatattaa acaagattta actaaggaga attgtaaaaa caaaaatgag aatttcatat
      481 aaagtttatt ctaggagtat gtatcttgaa taaaaattgt tatttaagtt ctgggggtta
      541 tgatgaatgt ttttataata ataaaatgca gctagaagaa aagttatttt aatataaatc
      601 actcaacata gcgagaaggc ggtttcctat atggaagtcg tcttgttttc atattgtcta
      661 aatacgagat atctactaca ggttatagtt gatttaaaca ggtctataga tttctaatat
      721 attaaggtga aataatatct tgtaatacat tgctatgttc tatgaaaaaa gcgacgatag
      781 ctaatgcaca agctcctata cttccacgat gtttgttgta aggtaaaatt tcaatttcta
      841 tattacgagt aaagggaacg aagtggagct ggtcttggat tttatccctg acttcatgag
      901 tgaaaggttg ataattaagc aattgactgt tgatatagat tttatcagca ggattgatga
      961 ggaggagatt tgcaatagaa gtggctaata aattgactcc tttatcaatt tgttgacgta
     1021 aagcggagtc gcctaaatta taagccgtta aaagggtgtc taaatgaatg tctttttcag
     1081 tctttacaag gctttttagt acagttagtt gggaattttt aaataataat tgggcgtgtt
     1141 tgattaacca agcatccgaa atatatgttt gtaaacaacc tttttttccg cattcacaat
     1201 attgcccatt caattcgaca atggtgtgtc caatttctcc gatataagga tttttagagg
     1261 ctcctatttt ttcttttgtg aaaaaggaag tgtaaatacc taatccagcg tgtaggaaaa
     1321 taaagttatc gggggtattg tgtggattaa aaaggtattg tcctatagcc atacaatcga
     1381 tattattttt tacaataaaa ggaaaattga atcttttaat tacatttaat aaattaaaag
     1441 attcccatat ggggttgttt gtaatgatac ttccggagtc tttgttataa tgtccaggta
     1501 ttgataagcc aaatccaagt aattttgttt ccaaagggac gtactctata gctgttttta
     1561 tcatattggt aatggtggaa ttgatttctg aaataggata atcttcattc aaaatttctg
     1621 tctcgaaaag agttttccct tgtaagtcac atagactgag aaagatacct aggacgttta
     1681 tttcaatacc tccaacaaca tacggaatat tatctgtgat agaaatagtt tttctacgtc
     1741 ttccagatcc ggaaaactcg ctaaattcat caccagtttc gtatatgagg gactgttcgc
     1801 ttagctcttt tatgatgtta gtcgttgttg ctggagtaag tcctgtttca ttaacaatat
     1861 ctgtacggga tgttgctgga tttcgatata gaaactccag tatagtggct ctattttgta
     1921 aaaaggctga ttgttttgta atcatttgtt tcctcctttg gtttatttta tattaaacat
     1981 aagaaaaaat caattaaaat tattattcta tacaaagaaa aaaatattgt aaaaacaaca
     2041 aaaaatatat tgacaacgct ttctgttagt ggtgtattta atttataaaa aataaaaaaa
     2101 ggagttccgg tgatgaacaa tattttagcg tttttagaaa caaaagtcgc tccgtttggt
     2161 gaaaaagttg gcaaccaacg gcatttgaaa gctattcgtg aaggatttat gatggcaatg
     2221 cctttgattt tagtcggctc tttatttctt attctaatca gttggcctca agaggctttt
     2281 acaaattggc tgaatagtgt tggattgcta agtatcttga caactatgaa tcagtcaaca
     2341 gtagcgatta tctccttggt cgcttgtttc ggtattgcct acaggttgtc ggaaggatat
     2401 ggtacagatg gtccgtcggc agggatcata gccttatcca gttttgtatt gatggcacct
     2461 cgtttttcga gtatggttta tgataaaaat ggggagcagg tcaagcagtt atttggcggc
     2521 gcaataccat tttctagcct gaatgcatct tctttgttta tggcgattac tattggattg
     2581 gttacagcag agatttatcg tatgtttatc cagcgcggaa ttacgataaa aatgccaagt
     2641 ggtgtcccag atgtagtaag taaatcattt tcagctcttt tatctggttt tactactttt
     2701 gttttgtggg ctttggtctt aaaaggtctt gaagcggcag gagttgcagg aggtctcaac
     2761 ggactcctag gtgcaattgt tggaacaccg cttaagttaa ttgcaggaac gcttccaggt
     2821 atgattctat gtgttattgt aaactcattc ttttggttct gtggagttaa tgggggacaa
     2881 gttttaaatg cttttgtaga cccagtttgg ttacaattta ctacagaaaa ccaagaagct
     2941 gtggctgcag gacaaacact ccaacacatt attacattac cgtttaaaga tttatttgta
     3001 tttattggtg gcggtggagc gactattggt cttgcgattt gtctcttcct atttagtaag
     3061 agtcgtgcga ataaaacatt aggtaagcta gctattatac cgtctatttt taatatcaat
     3121 acagctattc tatttacgtt tccaacagtt ttaaatccga ttatgctgat tccgtttatt
     3181 gctactccta caatcaatgc cttgattacc tatgtatcaa tggctgtagg attagtaccc
     3241 tatacaacag gtgtaatcct tccgtggaca atgccaccga ttataggagg cttccttgca
     3301 acaggggcta gttggcgagg agctctatta caagttgttt tgattttggt ttctgtagca
     3361 atttattatc cattcttcaa aattgcagat aaacgcaatc ttgaaaaaga aaaagctact
     3421 gttggaggga aataagatgg ttatcagagt atttgatcaa cagaaaaata cttattctag
     3481 ctttgcctta gaggaattaa gttactatat gaatcgggtc tttaagacta acatagagct
     3541 tgtcgaggag aaggaagcgg atatttttgt aggattagtc aataaagagg acagaaaaga
     3601 ccatgttctt atctcattag acaagggtaa ggggagaatt gagtctaata caattgtagg
     3661 tttacttatt ggaatttacc gaatgtttca tgaatttggg gttgtgtata ctagaccagg
     3721 gcgcagacat gactttgttc cagagttacg atttgaagat tttttagata aacagctatc
     3781 tatagatgaa acagccagtt actatcatag gggagtatgt atagagggag cggattcatt
     3841 tgaaaatata ctagatttca ttgattggct acctaagatt gggatgaaca gttttttcat
     3901 ccagtttgaa aatccttact cttttttgaa acgttggtat gaacatgaat ttaatccata
     3961 tctaaataaa gaacaatttt caaatgaatt agtacaagaa ttgagtgata ggttggataa
     4021 agaattgcaa aaaagaggtc ttattcatca tcgtgttggt catggatgga caggtgaagt
     4081 tttaggttac tcttcaaaat ttggctggga atcaggtctt agtatttcag aggagaagaa
     4141 accctatgtc gctgaaataa acgggaaacg agaattgttt aatacggctc cgattttaac
     4201 cagcctggat ttttcaaatc cagatgtagc tgataagatg gtagaaatta tcaaggatta
     4261 tgccaagaaa agacctgatg ttaactactt acatgtatgg ttgtcggatg ctcgtaataa
     4321 tatttgtgaa tgcgaaaact gtagacaaga attggtttcg gatcagtata ttcgtattct
     4381 caatcaattg gatagggctt taacgagtga gggattagat acaaagattt gttttctgct
     4441 ttatcatgag ttgttatggg cacctcagaa agaaaaatta gataatcctg aacgctttac
     4501 catgatgttt gcaccgatta caagaacatt tgaaatgagt tatgcagatg tagattttga
     4561 caattccata cctacgccta aaccttatat gcgtaataaa attatacttc cgaattctct
     4621 tgaggaaaat ttatcttatc tttttgagtg gcaaaaagca tttaaaggag atagtttcgt
     4681 atatgactat cctttagggc gtgctcatta tggcgattta ggctatatga aaattagtca
     4741 aactatttac agagatgtat cttatctttc caacctacat ttgaacgggt acatttcgtg
     4801 tcaagaatta cgtgccggat tccctcataa ttttcctaat tatgtcatgg gggaaatgct
     4861 ctggaagaag acaagaagtt atgaagaatt gattgaagaa tacttttctg ctttgtatgg
     4921 ggaaaattgg cagtctgttg ttgaatattt agaaaaatta tccatttatt cctcttgtga
     4981 ttattttaat gcaattggca gccgtcaaag tgatgtttta gcgaatcatt attatatagc
     5041 ttacaatcta gctgataatt ttttaccaat tattgaggaa aatatttcta agttattaaa
     5101 tagtcaaaag gatgaatgga aacagcttag ttatcatcgt gaatatgttg ttaagatggc
     5161 gaaggcttta tatcttcaag caactggaaa aacaaggcaa gctcaagatg aatggagaaa
     5221 tgtgttgaat tatatccgtg ggcacgaatt gctatttcaa tctaatttgg atgtttatcg
     5281 tgtaattgaa gtagcaaaaa attacgctgg tttccactta taaatcataa gtatagaaaa
     5341 tgaactaagg tattcagaga agattgatcc taaatattat gaaatttaag gatttttaag
     5401 atatttaggg tcaactttct atttatatcg tagcgaagtc attttaataa tgatgtgtaa
     5461 aagatggatc aagattgagg aggaagaaag atgaaatcaa aagaagaaat aaatatgctt
     5521 ggttttacaa ttgtcgctta cgcaggagat gcaaggtcag atttgatgga tgctttggcg
     5581 tttgcgagag atggatattt tgaacaggca agagaattgg ttgagtctgc aaacgactca
     5641 atagtgtctg cccatcgaga acagactaat ttattagcgg aggaggcata tggagataat
     5701 tttgaagtga gctttattat gattcatggt caagatactt tgatgacaac gatgctattg
     5761 tatgatcagg taaagttttt tattgatgaa tatgaacgaa ttcgaaagat tgaagaacat
     5821 attggtttgc aatgaggatt agtcatggaa aatttacagg ttaaagcctt accgaaggag
     5881 tttttattag gaactgctac cgctgcttat caagtagagg gtgcaactag ggtagatggc
     5941 aaaggaataa atatgtggga tgtttatttg caagaaaata gtccgttctt accagatcca
     6001 gctagtgatt tttattatcg ttacgaagag gatatagctt tggcggcaga acatggtttg
     6061 caggctttgc gtttatctat ttcttgggtt cgtatatttc ctgatataga tggggatgct
     6121 aatgtattag ctgttcatta ttaccataga gtttttcagt cttgcttaaa acataatgtg
     6181 attccgtttg tttctttaca tcattttgat tcgcctcaga aaatgttaga aacaggggat
     6241 tggttgaaca gagagaatat tgatcgtttc atacgatatg ctcgcttttg tttccaagaa
     6301 tttacagaag tcaagcattg gtttacaatc aatgaactga tgtctcttgc tgcaggtcaa
     6361 tatataggag gtcagtttcc tccaaatcat cattttcaat tatctgaagc aattcaagcg
     6421 aatcataata tgttgttggc gcatgctctt gcagtcctcg aatttcatca attagggatt
     6481 gagggaaagg taggttgtat tcatgcttta aagccaggct atcctattga tgggcaaaaa
     6541 gaaaatattt tggcagctaa acggtatgat gtttataata ataaatttct attagatgga
     6601 acttttttgg gctactacag tgaggacacg ctttttcact tgaatcaaat attggaagct
     6661 aataattcta gctttattat tgaagatggt gatttagaaa ttatgaagag agctgcacct
     6721 cttaatacga tgtttgggat gaattattat cgttcagaat ttattcgtga atacaaaggt
     6781 gaaaatagac aagaatttaa ttcaacagga ataaaaggac agtcttcttt taaattaaat
     6841 gctctaggtg aatttgtaaa aaaacctggt attccgacaa cagattggga ttggaatatt
     6901 tatcctcaag ggttatttga tatgttgctt cgtatcaaag aagaatatcc tcaacatccg
     6961 gtcatttatt taactgaaaa tggtacagcc cttaaagaag ttaagccaga gggcgagaat
     7021 gatattattg atgacagtaa gagaatccgt tatattgagc aacatttaca caaagtttta
     7081 gaggctcgag atagaggagt caatattcaa ggctatttta tatggtcttt gcaagatcaa
     7141 ttttcttggg cgaatggcta caataagcga tatggtcttt tctttgttga ttatgaaaca
     7201 cagaagagat atattaagaa aagtgctctt tgggtaaaag ggctaaaacg gaattaaggt
     7261 tagcgatttg actgatgttt aatatgtttt aaatatgagg ttgaattttt tataggagga
     7321 gttttatgga taagctagtc gctgccattg aaaagcaaca agggaaattt gaaaaaattt
     7381 ctactaataa ctatatgatg gctattaaag atggattcat tgctactatg cctttaatta
     7441 tgttttcaag ctttttgatg attattatta tgattcctaa aaatttcgga gtagagttac
     7501 cgagtccagc tattgtctgg atgagaaaag tgtatatgtt aaccatggga gttttgggta
     7561 ttattgtttc agggactgtt ggaaagtcat tagttggaaa tgttaacaga aaaatgcctc
     7621 acggaaaggt aataaatgat atttctgcaa tgttggcagc catatgtagt tatctggtat
     7681 taactgtaac gcttgtagtt gatgagaaga cgggatctac aagtttgtcg acaaactatt
     7741 taggatctca aggattgata acttcgtttg tcagtgcctt tattactgta aatgtttacc
     7801 gattctgtat taagcgagac attactattc atttacctaa ggaagttcct ggggctatat
     7861 cacaagcttt tagagatatt ttcccttttt cttttgtttt acttattagt ggtttgttag
     7921 atattgtatc tcggtttagt ttagatgttc cttttgccca agtatttcaa caactattga
     7981 ctcctatttt taagggggca gaatcatatc ctgctatgat gttgatttgg tttatgtgtg
     8041 ctttgctttg gtttgttgga attcatggac catctattgt cttacctgct gttacagctt
     8101 tgcaactgag caatatggaa gagaatgctc aacttcttgc aaatgggcag ttcccttatc
     8161 attctttaac acctaatttc gggaattata tcgctgctat tggaggaacg ggggctacct
     8221 ttgttgtacc atttattttg attttcttta tgcggtctaa acaattaaaa tcggtaggta
     8281 aagctacaat tactcctgtt ttatttgcgg taaatgaacc tcttctattt ggtatgcctg
     8341 ttattttgaa tccctatctt tttgtccctt ttttgatgac tccaccagtg aatgtatttc
     8401 taggaaaggt ctttattgat ttctttggaa tgaatggatt ttatatccag ttaccttgga
     8461 cctttcctgg tcccttggga ttgttaattg gaacgaattt tcaacttatc tcctttgtat
     8521 ttttatcttt gattttagtt gtcgacatat tgatttattt gccattctgt agagcgtatg
     8581 atagacagtt actggtgaaa gaagatattg caagctcaaa tgatattatt ttagaggagg
     8641 atacaagtga aataattcct ggtgagatag atgaaataaa aagtaaggag ttgaaagtac
     8701 tggttctttg tgcagggtct ggaacaagtg cgcaattagc caatgcaatt aacgaggggg
     8761 ctaacttaac agaggttaga gtgattgcga attcaggagc gtacggaact cattatgata
     8821 ttatgggtgt ttatgattta attattctgg ccccacaagt tcggagttat tatagagaga
     8881 tgaaggtgga tgcagaaaga ttaggtattc agatagttgc taccagagga atggaatata
     8941 ttcatttaac aaagagtcca agtaaagcct tacaatttgt attggagcat taccaagctg
     9001 tgtagtaagt ttttccatct tttatttgag taaagatttt gtttacagat aggcttggat
     9061 ttaaaaacgt tccccctttt ttaatataag aatccctctt tcacaattgt aaaaagaggg
     9121 attttgtatt ttatctctta gaccaagttc tcttcataaa gagaaggagg attgggtaaa
     9181 tctccaagcg ccctgcaatc attgcaaagg ataggagaat ttttgagatg ggactaaaga
     9241 ttgagaaact agaagtggtt cctagaatag gcccgatatt attgaaacag ctaaagacag
     9301 cgctggtcac gaccagaaaa tcattgctat ctaggctgac aataaagata agcgctagca
     9361 aaatcatagc atagatgaca aagtacttga gaatcttatg ctgggtatct ttgtcaatca
     9421 ccgttttatt aacatggagg gtcaaaacac ggtggggcga taggattgac aaaatttggt
     9481 ttttggcaat ttttgaaagg atgaggcctc gaataatctt gagtccacct gcagttgatc
     9541 cagcagagcc accgattgcc atgaggaaaa ggaggataaa ctgggagaag aggggccagt
     9601 tggtaatatc tccatatcca aaaccagttg ttgtaatgat gttggaaacc tggaagaagg
     9661 tcatttcaaa gctctttgaa aaccctgggt agaggtagag ggtgttgagg ctaatcaagc
     9721 ctgtagaaac cagtacaatg accaagtaag ccctaagctc ttcatctcca aagaaggcct
     9781 tgatgcgacg gagcatgagg tagtagtaga ggttgaaatt tactccaaaa accagaactc
     9841 cgatactgac cagataggta atcagtgagc tgccatagtg ggcaattccg tcgttataga
     9901 cggtaaagcc tccagttccc gctgtcccca tagcaataac aaaactatcg tagagaggca
     9961 taccggctag ataatagatg atgacaaaga gggagaagag agctagataa aggagataga
    10021 gaatctgggc agtgtttttt agtttggata caaccttgcc aaaaacagga cctggaacct
    10081 cagccttcat cacctctagg tggctatttt tggcattgtc cataatagca agtgcaaaaa
    10141 caagcactcc catccctcca atcaagtggg taaaacttcg ccagaagagg agggaacggc
    10201 tgagaaccga aacgtcgttc aaaatacttg ctccagtagt tgtaaatcca gaactaattt
    10261 caaaaaaggc atcaataagg ctggggattt gcccagaaaa gacaaagggg agaccaccaa
    10321 agaaagacca aaggatccaa cagagggcaa cgatcaagac tccctccttg gcataaatcc
    10381 gttgattttt tggcttctgt aaactccctg aaccgcctaa caatacgaga atccctatgg
    10441 tcgaaaagag ggctgtaaag acttggctcg attcacggta atagacagca atcgcaacag
    10501 gaaccaaaag aagaacagct tcaatcaaaa gtaattttga aaggaggtaa cgaatcatac
    10561 ttttattcat ttcttacctc gcgatcaagt cataaatctt ggtgatgttt ggcaacaagg
    10621 ttgttactag gagcttgtct ccaacttcca acatatcctc cccagttggg aaaatagtct
    10681 tgccctttcg aataatggct gcaataagaa cccctttttt caatttcagt tgagaaagag
    10741 gtttggcagt cattttattg gcttccttga tatggaattg cagggtttcg atttggccat
    10801 tggctagatg gtgcatagct tgaaggtctg aatactgggc attaactcga ccacgaataa
    10861 agtgcataat cgtatctaca gcgatgcttt taggtgtgat gatacttgaa aaatcaggcg
    10921 cattgataat ctcgaggaga ctggtacgat tgaccttagt aatatttttc tgtacaccta
    10981 ccctgtcaag gaacatagat gtaatcagat tttcctcatc gactcctgtt agagtcgcaa
    11041 cggcatcata gtgttgagca ctttcttcca gcaggatatc ttttgcggtt ccatctcctt
    11101 gaacgatgta gagatttggg aatttctcgc taaagaagct ggcgatttca ggattgattt
    11161 caatgacttt tgtatcgata cgactatctt tgagaatacc aagtagataa taggcaattc
    11221 tacctgcccc aacgatgaga aggctcttca cggcgcgtga tttaaaataa ttatggaaga
    11281 gtatcatatc gacacggtta ccagtgacaa agattctatc tttatcctgt acagtcatgt
    11341 caccgcttgg aatgataatt tgatgatccc tctctatcgc acagacaatg acattaccaa
    11401 attttttacg aaaatcagaa atgggcattt ggcaaagacc gctggtggac ttgacgacaa
    11461 attccatgag gctaacgcgt ccaccagcaa agcgttcgac agacagggcg ttggggaagt
    11521 caatgatatt cgcgatagcg cgggcagcca agagctcagg attaacgata agagaaaaac
    11581 cgagaatatt cttttccttg aaataagagt tagaatattc agggttccgc acccgaacga
    11641 tagtttcttt agctcccatt ttcttggcta gaactgctgc aatcatgttg acttcatcgt
    11701 gctcagtcag ggcgataaag atatcacaat cttggacgct ggcttgctca agaatggcaa
    11761 aatcggcccc gttaccaagg ataccaatga tatcaaagcg actgacaata tgattgagaa
    11821 cagcttcgtc ttgctcaatc agcaaaacat catgcttttc tgcaaccaag gagcgacaga
    11881 gggcaaaacc aacttttccc cctccgacaa ggataatttt cataataaaa cctacttttt
    11941 catgatgtaa ctatcatacc ctttttcaag aaaaaatgca cctactagct aataacaaga
    12001 gtttttagtg aaaattcgct ataaggtaaa actataccct aaccaattga aatagctatt
    12061 agcgactttc tctgaaatat ggtatgataa aggatataca aggagataaa atgaataata
    12121 atttactggt attacaatca gactttggtc tggttgatgg tgcggtatcg gctatgattg
    12181 gagtggcttt agaagagtct ccaaccttaa aaatacatca cttgacgcac gatatcacgc
    12241 cttataatat ttttgagggg agctatcgtc tctttcagac ggtggattac tggcctgagg
    12301 gaacgacgtt tgtatcggtt gtcgatccag gtgtcggttc gaaacgtaag agtgtagttg
    12361 ccaagactgc aaaaaatcaa tacattgtca cgccagataa tgggacgctt tcctttatca
    12421 agaaacacgt tggcattgta gccattcgtg agatttctga ggtggccaat aggcgtcaaa
    12481 acacagagca ttcttatacc ttccacggtc gtgatgtcta tgcctatact ggtgctaaac
    12541 tggccagtgg tcacattact tttgaggaag tagggccaga gctcagtgtg gaacagattg
    12601 tagagcttcc agtcgtagcg accatcatag aagatcatct ggtgaaggga gccattgata
    12661 ttctggatgt gcgtttcggt tcgctttgga cctctatcac acgggaagaa ttttacaagc
    12721 tggaaccaga atttggtgat cgttttgaag tgaccatcta tcatgctgat atgctggtct
    12781 atcaaaatca ggttgtctat ggcaaatcat ttgcagatgt gagaattggg caacccatcc
    12841 tttacatcaa ctctctctat cgtttaggtt tggctatcaa ccaaggttcc tttgccaagg
    12901 cctataatgt aggtgtcggt tcatcttgga ccattgaaat aaagaaaata gaaggataaa
    12961 ataggagaat agtatggaaa tcaaatttac aattaaacaa gttgttgctg tcggaattgg
    13021 cgctgccctc tttgtcgtca tcgggatgat caacattcca acccctgttc caaatacaag
    13081 catccagctt cagtatgcgg tacaagcgct actttctatt atttttggac cgattatcgg
    13141 tttgcttgtc gggttgattg gtcatgcaat caaggactct cttgttggtt atggtctatg
    13201 gtggacttgg attatcgcta gcggactctt tggtttagtt gtgggacttt ttagaaagta
    13261 cgttcgcgtg atcaatggtg tttttgactg gaaagatatt cttattttta atctcattca
    13321 actacttgca aatgcccttg tttggggtgt cttggcacca cttggagatg ttgtgattta
    13381 tcaagaagcg gcagaaaaag tatttgcaca agggattgtt gcgggaattg ccaatggtgt
    13441 atctgtagct attgcaggaa ctcttctctt acttgcctat gcaggaaccc aaactcgtgc
    13501 aggaagtttg aaaaaggact aatactcaat gaaaatcaaa gagcaaacta ggaagctagc
    13561 cgcaggttgc tcaaaacagt gttttgagat tgtggataga actgacgaag tcagctcaaa
    13621 acatggtttt gaggttgtag atgaaactga cgaagtcagt aaccatacct acggcaaggc
    13681 gaagctgacg tggtttgaag agattttcga agagtataaa atgatgggga aggctgggca
    13741 actagtcttt ttcgatgttt atagattagt taggcaagta agctaggata ggaatctttt
    13801 tggcgctaag attttaagag gagagactgc aagaagggct tgatttatga taaaatagaa
    13861 gtctaagaag cgaatgaaga gagtaagatg aaagaagcta taattgagtg gaaggatttc
    13921 tctttccggt atgaaacaca acaagaaccg accttgcaag ggatagactt gaccatttac
    13981 aagggagaga aagtcttaat tgttggacca tctgggtcag gtaaatctac cttgggtcag
    14041 tgtttgaatg ggattattcc caatatttac aagggtcaga catatggaga atttttgata
    14101 aagggtcaag tagcctttga tatgagcatc tatgataagt ctcatctggt tagcacagtt
    14161 ttgcaggata cagatgggca gtttattggc ttgtctgtgg cagaagattt ggcgtttgct
    14221 ctggaaaatg atgtgacagc cctagatgag atgaaaggtc gtgtttataa atgggctgaa
    14281 aagctggacc ttcttccttt actggatcag cgtcctcagg atttgtcagg tggacaaaag
    14341 cagcgagtca gtctggctgg tgtcttgatt gatgaaagtc cgattctctt gtttgatgag
    14401 ccactcgcca atctagatcc caagtcaggt caggatatta tcgaattgat tgaccagatt
    14461 cataaggaag aggggacgac gactcttatt atcgagcacc gtttggagga cgttctgcat
    14521 cgccctgtgg atcggattgt cttgataaac gatggtcgta tcctttttaa tgggagccct
    14581 gaccagttgc ttgcgactga tttattgact caaaatggaa ttcgagaacc cctttatcta
    14641 acgactctcc gtcaattagg tgtggactta gtcaaggaag aacaattagc gaatctggat
    14701 aacttgtcta tctcaaaagg tcaggttcag ttgcagaatg aactggcaaa agaaacccca
    14761 gcattgcagt cactctttag actagaggaa gtatcttttt cttatgatga tagaccgatt
    14821 ttaaaatccc tacatttaga tattaaaaag ggtgaaaaga ttgctattgt cggaaaaaat
    14881 ggagcaggga aatcaactct agccaaggct ataagtagct ttattcagac ggaaggacgc
    14941 tatctttggg aaaaacagga tataaaaggc gattctgttg cagagcgggc ggaacgagta
    15001 ggatatgtgc tacaaaatcc taatcaaatg atttcaacca atatgatttt tgatgaggtg
    15061 gctctagggc tccgtttgcg aggtgtggat gagaaggaaa ttgaaacgag agtatatgaa
    15121 accttgaaaa tctgtggact ttatgaattc cgtaattggc ctatttctgc cctgtcattt
    15181 ggtcagaaaa aacgtgtcac cattgcttca attttggtct taggagctga aattattctc
    15241 ctagatgaac cgactgcagg tcaagatcag aagaactata ctgagattat ggaatttctc
    15301 gaagagttac atcaaaaagg gcataccatt gtcatgatta cccatgatat gcaattgatg
    15361 ctggattatt cagaccgggt ccttgtcatg gtggatggag aattgattgc cgatactgtt
    15421 ccagccagtc tgttgagcga tcctgagctg ttagtaaaag ccaatctaaa agaaacctcc
    15481 atctttaact tggctaagaa actagatgtg gatccactgg atttaacggc attttacaaa
    15541 gaaaggagag agggatgcaa gctaaattaa tcggttacca gcatagagat actgtgattc
    15601 atcgcttgtc aggagctggg aaacttctct ttttcattct ggtatcattg gcggccatga
    15661 ttagctatga taccagaatg ctcttgctga ttgccatctt ttcagtcttt ctcctctatt
    15721 tatcagaaat tcgctttaaa gatgtttcct ttgtagccgt ttttgcgacg atatttgccg
    15781 ttttaaacgt tttgatggtc tatctctttt ctcccgagta tggggttgga ctttatggag
    15841 agagaagtgt gatttggcag ggaatcggtg tctacactct aaccagccag gagctctttt
    15901 atctgctaaa tttggccatt aagtatcttt gcaccattcc tctggctatt atctttttga
    15961 tgacaaccca tcctagtcag tttgcttcca gtttaaatca aattggtgtg ccctataaga
    16021 ttgcttattc tgtcagcctg accttgcgct atattccaga tttgcaggaa gaattcttta
    16081 ctatcaagat gtctcaggag gcgcgtggga tggaattatc caagaaagct tctcttatgc
    16141 aacgaatcaa aggcaatctg ctcattatta cgcccttgat ttttagctcg ctagaacgca
    16201 ttgataccat tgcgactgcc atggagcttc gccgctttgg gaaagagaaa aaacgcacat
    16261 ggtatagtta tcaggccttg aaaaaaggag actatattac cttgctcttg gcaaccttgt
    16321 ttttagtagt tagtttacta cttatcttgc agaatcaggg acgattttac aacccttgga
    16381 aatagcttga aaaaattgaa aaaatcaagt catttctatt gacaatgatt ctgaaagtgt
    16441 tatactaaga aagtagtttc gctgatttac ttcaaacctg ttgtgaggta agttaacgat
    16501 gccttaacca cgctgtttgc tgagcttgac tccgggcagt gtggctattt ttttgcaatg
    16561 gtgaaaggaa gcaagtcatg acaaatcaca ttgtattatt tgaacctcaa attccacaaa
    16621 atacgggcaa tatcgcgcgt acttgcgctg cgaccaattc tcccctccac atcatcaagc
    16681 ctatgggctt tcctattgat gaccgcaaga tgaagcgggc tggattggat tactgggata
    16741 agctagagat ttatttttac gagagtttgg aagatttcat atctcagatg aagggaaaac
    16801 tctatctgat ttcgaaattc gcggaaaaag tctattctga ggtggattta tcgactgacg
    16861 aggaccatta ttttctcttt ggacgtgaag acaagggctt acctgaagat tttatgcgag
    16921 aacatcctga gaaggctctc cgtattccca tgaatgatga acatgtccgc agtctcaatg
    16981 tgtctaatac cgtctgcatg attgtctatg aggctcttcg ccagcagaac tttgcaggtc
    17041 ttgagctcgt ccatacctat gaagtggata aattgaaata aaaaatgaaa atgaacaaaa
    17101 tgcttgcgct tgcaagcgtt ttttgttatg ataaaagagt cttcagggca gggtgaaatt
    17161 cccgaccggc ggtgacttta actaggaaat gatcttttcc ttttatactt tgttgacaag
    17221 ctttgcctaa ccagaagtta tgcctacagc ttgtcgccta gtctaaaaga aaaatctcta
    17281 tttccttctc tttaaagaag tccgtgagcg caagctgatg tggtgagatt ccacaaccga
    17341 cagtatagtc tggatgggag aagacgaaag aatagccttg tctgttttga tagatttata
    17401 gccggattgt aaccacttgc tttgtttttc tttaatagag taagagggaa cttttgggat
    17461 ataaaaagtg agaatagata gaggaatcct ttccaacttc ttctgatttt atagaaaatt
    17521 ggaggaacct gttatgacaa acacacgtcg actttcgacc attgcaattc tatcagccat
    17581 ctcatttgtg ctgatgtact ttgactttcc gcttttacca gcggcatctt tcctcaagat
    17641 cgaatttagt atcttgccag tccttgtggg tctggtggtc atggatttgc ctgctgctct
    17701 aggagtgctc ttgcttcgct cactcttgaa attgcttctt aacagccaag gagtgaatac
    17761 ttacattggt ttgccgatga atatcgtagc tttgggagtt tttgtcatcg tatttgcttt
    17821 gatttggaaa aaggaacgga caacccttcg tttcctacta ggctctctag ctggaactat
    17881 tggtttaacc ttggccatgt tggttctcaa ctatgtttat gctgttcctt tgtacgctaa
    17941 gtttgctaac tttgatattg gaaaaatttt gggactttcc aactacctaa tgaccatggt
    18001 attacctttt aacttgattg aaggtgttat cttttccgtt tcattctggt tgttgtatgt
    18061 tctcttgaaa ccaaccttaa aacattatga gagataaaca aacattttta atgaagggca
    18121 gttttgccct tttacttttc gttattcttg gctacatggt caaattttac cctgaaatgt
    18181 tggtcaattt tgaccaatcg attcagactg ccattcgagg agacttacca gattatttga
    18241 ctattctttt tcgagccctc acacgtctga ttgatatccc agtgattatc acttgggttg
    18301 tcattacagc ttttgtcttt tatcgtaagc gatggaagat agaaagtttc ttcatgctgg
    18361 gaaatctggc tttggcaggt cttttaatcg tgacctttaa aaatatctac cagcgcccac
    18421 gaccagctat tttacatctg gtggaggaga agggattttc cttcccaagt gggcattctc
    18481 tagctgtaac gctgatggta gggactttga ttgttattct tagtcagcgg attaaagatc
    18541 aggtctggag aaaaatcgta caaatcgtcc ttggcctcta cctagtcagt gtgctggtat
    18601 caagagtcta tctgggagtt cattacccat cagacgtcct tgccagtctc tgtgtgggct
    18661 tgggagtcct gtttatcgag tttccctttt atgacaagct ccgcttccaa tggcgattta
    18721 aaggcaaaca gaagtgagta tcgaattccc ttgaggagaa agaaatgaaa gtcaatatag
    18781 cagatcttca tctgactcaa ctatatttat cagaaaagaa gttgcaagat attcagatgc
    18841 tttaccagtc ggcagaaaca atccaagtcg atccaatcag tattcttgcc tttggagatt
    18901 gtttgctgat tacagatggg catcacaggg cttatcaggc tttattggca ggtcgggata
    18961 cgatttctgc tgagtgggat agagatggtg gtgatgaact atatcatctc tatgcgcaag
    19021 cttgcgagga aagaaagatt tactctgttt ttgatttaga agatcgtatc ctagctcaag
    19081 atgggtatga agcaaaatgg tataactggt gtgatggttt taatcaagca gcaactcttt
    19141 tgttgaaaag gtagtgcatt gtgttttgtt ccttattata aggtcaatca ttgtgaggaa
    19201 gcttttgctt ggtatcagga tgtgaacttg gtttacctcg tagatggggt gaagcttcct
    19261 tacagtcaag cgaccttgga agccatgtat tcctatttgg atcggcatgg tgagcttttt
    19321 tggattgaag tcaaggagaa gggagaatgg tttccaattg gggatattac actatctcag
    19381 gataatctcc ccattgtgat tgggaattcc gcttaccaac atcgaggact tggaaaaaag
    19441 attctaagtg ctttgattga attggctcga gtaaaaggat ggaaagaatt gagagtcaag
    19501 aaaatctaca cctacaatca tgcttctagg aggtgtttca agtcgcttgg atttgtggaa
    19561 aatggagcaa cagaaaaagg aatgagtttt atattggaat tagtctaatc ctgccacttt
    19621 ctccccctta acctttgact attccgcaaa attatcgtaa aataaagagt aaatgataaa
    19681 atgaggtcag agtctgttcg ctctggcgat agtagtataa atgaggagaa acgctttgga
    19741 attagaagta tttgctgggc aagaaaaaag tgaactatct atgattgagg tagcgcgtgc
    19801 tatcttggaa cttcgtggtc gcgatcatga gatgcatttt agcgatcttg taaacgaaat
    19861 tcaaaactac cttggaacat caaacagcga tatccgcgaa gctttgcctt tgttctacac
    19921 agagttgaac tttgacggta gcttcatctc acttggggac aacaaatggg gtcttcgttc
    19981 atggtatggt gtggacgaaa tcgacgaaga aatcatcgct cttgaagaaa atgacgacga
    20041 tgaagtagca ccaaaagcta agaaaaaacg tgtcaatgcc tttatggatg gtgattcaga
    20101 tgccattgac tacaatgcag atgatccaga agacgaagat gcatacgaag cagatccagc
    20161 tctttcatac gatgatgaaa atccagatga tgaaaaaaat gaagtggaag cttatgatgc
    20221 agaaatcaac gaaatcgctc cagatgactt gggagaagat gtggatctca acgaagacga
    20281 cgacgagttt tcagatgacg atgctgaaac cagcgaggaa taagagttag ctattgacaa
    20341 ttatcctatt tttaggtatc atattgtttg ggcacctctt ttagaggtcg gggctcccta
    20401 gttcttaggg agctattttt gttttttcaa gaagttatct tcttgtattt tatactcaat
    20461 gaaaatcaaa gagcaagcta ggaaactagc cgtaggctgc tcaaaacact gttttgaggt
    20521 tgtagataag actgacaaag tcaggaacac atatctacgg caaggcgacg ttgacgcggt
    20581 ttgaagagat tttcgaagag tattagttgt gaatctggtg cagtcgtccc agattattct
    20641 tattagtagg gtcttgtttt ctatatcccc tcgtagttaa caagaccttg agcattttag
    20701 aaagaggaat ctatgtctac gaaatatatt tttgtaactg gtggtgtggt atcgtccatt
    20761 gggaaaggga ttgtggcagc gagtctaggc cgtctcttga aaaatcgtgg tctcaaagta
    20821 accattcaaa agtttgaccc ttatatcaat attgatccgg gaaccatgag tccttaccag
    20881 cacggggaag tttttgtgac agatgacgga gctgagacag atttggactt gggtcactat
    20941 gaacgtttca tcgatatcaa tctcaacaaa tattccaacg tgacaactgg gaaaatttac
    21001 agtgaagttc ttcgtaaaga acgccgtgga gaataccttg gggcaactgt tcaagtcatt
    21061 cctcatatca cagatgcttt gaaagaaaaa atcaagcgtg ccgctctaac gaccgactct
    21121 gatgtcatta tcacagaggt tggtggaaca gtaggagata tcgagtcctt gccattccta
    21181 gaggctcttc gtcagatgaa ggcagatgtg ggtgcggata atgtcatgta tatccataca
    21241 accttgcttc cttacctcaa ggctgctggt gaaatgaaaa ccaaaccaac ccaacactct
    21301 gtcaaagaat tgcgtggctt gggaatccaa ccaaatatgt tggttattcg tacagaagag
    21361 ccagctggtc aaggaattaa aaataaactg gcccagttct gtgatgtggc accagaagcc
    21421 gttatcgaat cgttggatgt tgaacacctt taccaaattc cactgaactt gcaggcacaa
    21481 gggatggacc aaattgtttg tgatcatttg aaattagacg caccagcagc ggatatgaca
    21541 gaatggtcag ccatggtgga caaggtcatg aacctcaaga aacaagttaa gatttccctt
    21601 gttggtaagt atgtggagtt gcaagatgcc tatatctcag tggtcgaagc cttgaaacac
    21661 tctggctatg tcaatgatgc agaagttaaa atcaattggg tcaatgccaa tgatgtgaca
    21721 gcagagaatg tagcagaact cttgtctgat gcggacggga tcatcgtacc aggtggtttt
    21781 ggtcaacgtg gtacagaagg gaaaatccaa gccatccgct atgcgcgtga aaatgatgtt
    21841 ccaatgttgg gagtctgctt gggaatgcag ttgacatgta tcgagtttgc tcgtcacgtt
    21901 ttaggtcttg aaggtgccaa ttctgcagag cttgcaccag aaacaaaata ccctatcatt
    21961 gatatcatgc gtgatcagat tgatattgag gatatgggtg gaacccttcg tttgggactt
    22021 tatccgtcta agttgaaacg tggctctaag gctgctgctg cttatcacaa tcaagaagta
    22081 gtgcaacgcc gtcaccgtca ccgttatgag tttaataatg ccttccgtga gcagtttgag
    22141 gcagcaggtt ttgtcttttc aggagtttct ccagacaatc gtttggtaga aatcgtggaa
    22201 attcctgaaa ataaattctt tgtagcttgt cagtatcacc ctgaactgtc aagccgtcca
    22261 aaccgaccag aagaactcta cactgccttt gttactgcag cagttgagaa cagcaattag
    22321 caaaatcaga acctttgaga aaaatctcag aggttttttg catacgatga tattgcagta
    22381 tatctgaggt aggggtcctc tgtatgtacc tgctaccgtt gaaatcaata gcgactccct
    22441 cttgccctgt gctagtgaat ggatttatca gtatattgaa atgaaataaa atttgaacaa
    22501 attaattcgg aaagccaaat caatttctag caaagtttta ggaactggat tgtatagtga
    22561 attgaaataa gatgtgaaca tctctatcag gaaagtcaaa ttaatttata gaaatatttt
    22621 agcagtcaag atgtactgtt atagattcaa tacattatac ttttttaatt taatccacta
    22681 tagtaaaatg aaataataac aggacaaatc gatcaggaca gtcaaatcga tttctaacaa
    22741 tgttttagaa atagaggtgt actattctag tttcaatata
//
LOCUS       DAVKNL010000029        22724 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVKNL010000029 DAVKNL010000000
VERSION     DAVKNL010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22724
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.029"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            1..463
                     /locus_tag="VW411_001169"
                     /pseudo
     CDS             1..463
                     /locus_tag="VW411_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841243.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(764..2653)
                     /locus_tag="VW411_001170"
     CDS             complement(764..2653)
                     /locus_tag="VW411_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEU9793900.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKIQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSSFEKDCLNLAFSGKKEETLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGGEKALQVGMG
                     ALTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDQAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSTFYHSTAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            2791..4356
                     /locus_tag="VW411_001171"
     CDS             2791..4356
                     /locus_tag="VW411_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU9793901.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYN
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKVIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            4356..5096
                     /locus_tag="VW411_001172"
     CDS             4356..5096
                     /locus_tag="VW411_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9793902.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(5205..5420)
                     /locus_tag="VW411_001173"
     CDS             complement(5205..5420)
                     /locus_tag="VW411_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793903.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
     gene            complement(6155..7405)
                     /gene="ilvA"
                     /locus_tag="VW411_001174"
     CDS             complement(6155..7405)
                     /gene="ilvA"
                     /locus_tag="VW411_001174"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEU9793904.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIKTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(7455..7811)
                     /locus_tag="VW411_001175"
     CDS             complement(7455..7811)
                     /locus_tag="VW411_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU9793905.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEVFGDE"
     gene            complement(8049..8285)
                     /locus_tag="VW411_001176"
     CDS             complement(8049..8285)
                     /locus_tag="VW411_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793906.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMNFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKH"
     gene            complement(8392..9414)
                     /gene="ilvC"
                     /locus_tag="VW411_001177"
     CDS             complement(8392..9414)
                     /gene="ilvC"
                     /locus_tag="VW411_001177"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU9793907.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(9480..9956)
                     /gene="ilvN"
                     /locus_tag="VW411_001178"
     CDS             complement(9480..9956)
                     /gene="ilvN"
                     /locus_tag="VW411_001178"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEU9793908.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(9949..11649)
                     /locus_tag="VW411_001179"
     CDS             complement(9949..11649)
                     /locus_tag="VW411_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEU9793909.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            complement(11849..13516)
                     /locus_tag="VW411_001180"
     CDS             complement(11849..13516)
                     /locus_tag="VW411_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEU9793910.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDAVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(13519..13884)
                     /locus_tag="VW411_001181"
     CDS             complement(13519..13884)
                     /locus_tag="VW411_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9793911.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(14041..14229)
                     /locus_tag="VW411_001182"
     CDS             complement(14041..14229)
                     /locus_tag="VW411_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEU9793912.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(14345..14521)
                     /locus_tag="VW411_001183"
     CDS             complement(14345..14521)
                     /locus_tag="VW411_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEU9793913.1"
                     /translation="MDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     DGMWRAENVTQDLI"
     gene            complement(14632..14829)
                     /locus_tag="VW411_001184"
     CDS             complement(14632..14829)
                     /locus_tag="VW411_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEU9793914.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNV"
     gene            complement(15112..16656)
                     /locus_tag="VW411_001185"
     CDS             complement(15112..16656)
                     /locus_tag="VW411_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEU9793915.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLETFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMNPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            16869..17171
                     /gene="gatC"
                     /locus_tag="VW411_001186"
     CDS             16869..17171
                     /gene="gatC"
                     /locus_tag="VW411_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEU9793916.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGIDRDRLFKNVPEKDNYYIKVPAILDNGGDA"
     gene            17171..18637
                     /gene="gatA"
                     /locus_tag="VW411_001187"
     CDS             17171..18637
                     /gene="gatA"
                     /locus_tag="VW411_001187"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEU9793917.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWNHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGIDPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            18637..20079
                     /gene="gatB"
                     /locus_tag="VW411_001188"
     CDS             18637..20079
                     /gene="gatB"
                     /locus_tag="VW411_001188"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEU9793918.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVKLEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            20208..20768
                     /gene="efp"
                     /locus_tag="VW411_001189"
     CDS             20208..20768
                     /gene="efp"
                     /locus_tag="VW411_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU9793919.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            20790..21179
                     /locus_tag="VW411_001190"
     CDS             20790..21179
                     /locus_tag="VW411_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9793920.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            21172..21594
                     /gene="nusB"
                     /locus_tag="VW411_001191"
     CDS             21172..21594
                     /gene="nusB"
                     /locus_tag="VW411_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU9793921.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(21629..22724)
                     /locus_tag="VW411_001192"
                     /pseudo
     CDS             complement(21629..22724)
                     /locus_tag="VW411_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT         6695 a         5127 c         4197 g         6705 t
ORIGIN      
        1 ttgaggcagg atttgataag ctgatagaaa ttaagcgatt gaccgcttct aaaattcagg
       61 atatcctctc agtcgcacct cgttctatcg gaacaacatc tcctgctcgt gaattcgaaa
      121 tcatcaagca ttacaagagg ctcattgaca aggcggaaac atgtatcaat gactcgatgg
      181 ctgagttcaa ctcggtcatc acgacggtta ctgggattgg ggatcgttta gggcggtcat
      241 tttagccgag attcgaaata ttcatgcctt tgataatcct gctcaattac aagctttcgc
      301 tggactggat tcttctattt atcagtcagg tcagattgat ttagctggaa gaatggtcaa
      361 acggggttcc cctcatctgc ggtgggcact catacaagct gccaaagcat gccctcgctt
      421 ttcacctgct tctaaggcct atcttaagac taagttagaa taaggaaaac attacaatgt
      481 agccatcatc caccttgcaa aaaaacttat ccgaaccctg ttttatatcc taaaaaagag
      541 ctgccatttg acgaacaaaa agtgatgtaa tttctgaaat taacacagga ttacctttga
      601 aaaaatggac tttttcaagg gctttttgcc gtgctctaaa atcatcgcca aatcatcaaa
      661 ttgaaataag aaatagttta gataaaacta ttgacttttc ttatagtttg tcttttttct
      721 atcttccatt atatcatact ttttcagtct atggtagctc tctttaaaag gcaccgatac
      781 tgccgccacc tccgcctcca gagaagccac caccagaact tccacttcca gaagatacag
      841 agtaggtgct tgctgtattt gcgacactag cataatggct catttgtgct gttgaatgat
      901 aaaacgtact gtgccagcca taagctacat agagattgat atctggattt tccacttgaa
      961 tctgatgaac cttcatcaaa tgactaacct tgtccgcata gccaaataag gtcgcataga
     1021 ccaagaggcg attccagacc acaatacttt ccagttcagc ctgatccaat cgtgcaatct
     1081 cacgcaacat attttcaaaa ctggtccaga gatagtagac ctcagctccc gcttcattta
     1141 gaacaccatc acgattatct agtcgaagct tccaataata gaaaacagac aaaactaacc
     1201 ctagaaaacc aagtattggc aaagggaggt aaagatagcc atgaacgtct aaactgtaca
     1261 agaacaaacc aaatccgata aataggggca ggatagtcaa ggcacccata cccacttgca
     1321 aggccttttc cccaccagtt aaaggacgat aataatctgg gagcccccag aaggaaactc
     1381 gttttctcac tccttcttgc atctggttca atacctcttc aaaagaagat ttgagttgaa
     1441 gccctcttgc ttgaatccgt ttttcatcag aaactttggc tctacgataa agactatcag
     1501 ataccttgta atccgcaaac aaattggaaa gagtttcttc ttttttacct gaaaaagcta
     1561 gatttaggca gtctttctca aagcttgaca aaccatcttc ttttactagc ctcaaaccaa
     1621 ctgcatctcc ttctgaaatg atagagacat tcccacggtc tatcacatct agcaaggtag
     1681 cttgaataag ttgatcaaag gtgaattttc cagctccctt gaccaaggga ctcacttcct
     1741 ccaaggaggt cgagtagact gcttctgata aaaccatagg ctctaattcc attggtggtt
     1801 catagagacg atgatttttg gcatatttga ctgaaggagt ggtctttctt ctataaataa
     1861 aatagaagca gacactcaat aacaaggaga tggaaaggat cgaagggagg acccaagtaa
     1921 cgagttgttt actctgatct ttttctctaa caatcgagtc ttctatctta ttaaactctt
     1981 ctaaacgatt ccctttcaat ccctgatccc tagcgctagc aaaatcggtc cgaggccaat
     2041 aggcatgcaa ctcaactcca cgcttagccg gaagattgtc taaacggata gtataatcaa
     2101 ggttactctt ttcaatcgtt ccctctctaa aaagtttccc tgtatggaaa aagagttttt
     2161 cagccccctt gtctcccctt acatgaaatt caaacttttc aatagactct gaactatctg
     2221 tcagaggttg ccaatttaat tcagcgatat catcataaag gaaaagtaaa ttttttaagt
     2281 tccagacgag gtcaacttca actatgtcgc cctcctgacc tggattatag actctcacag
     2341 tataaccatc cgcttcttct gttacttcgc tagtcacatc tgctagttct gcaccgtttt
     2401 tcgcggcctg aatctttgga tgagggtcaa tgtcaaaccc gctaggcatc ttaccagcac
     2461 gtccaagtcc cacgatttgg cccttaaagt cctcctcaaa ctggtaaact atcttctgtc
     2521 taaactctgc cgtattgtct gcatgaatat acaaatcccc ttgataagag tttatcttga
     2581 aatcaatggc aaaaacagag agtggaagaa ggcaaaacaa gcctaacacc agtaagaaaa
     2641 aagttttttt catcaactaa gccccctttt tatctttctt atcattatag cattttttcg
     2701 taagtaaaga cttacttatg ttgaaaaatt acgcattttt cgataaaata ggagacagtt
     2761 attttttaat agatcagaat aggagaaatc atgagaaaaa tatacttatc tattttcaca
     2821 agtctcttgc tgatgctagg acttgtcaat gttgctcaag ccgatgaata tttacgcatc
     2881 ggtatggaag cagcatatgc tccctttaac tggacccagg atgatgatag caacggagct
     2941 gtcaaaatcg atgggaccaa tcagtatgcc aacggatacg atgttcaaat cgccaagaaa
     3001 atcgctaagg acttaggtaa agaacctttg gttgttaaaa ccaagtggga aggtctagtc
     3061 cctgccctta cttctggtaa gattgacatg attatcgcag gtatgagtcc aactgcagaa
     3121 cgcaaacaag aaattgcctt ttcgagcagt tactatacta gcgaaccagt tttgcttgtc
     3181 aaaaaagatt ctgcctacgc aagtgctaaa tctttggatg actttaacgg tgcaaaaatc
     3241 acttctcaac aaggggtcta cctttataac ttgattgcac aaatcccagg tgctaaaaaa
     3301 gaaacagcca tgggagactt cgctcaaatg cgacaagctc ttgaggctgg tgtcattgat
     3361 gcttatgttt ctgaacgtcc agaagcactg actgctgaag ctgcgaactc taagttcaag
     3421 atgattcaag tagaacctgg tttcaaaact ggggaagaag atacagctat cgctatcggg
     3481 cttcgtaaaa atgacaatcg tattagccaa atcaatgcca gcattgaaac catttcaaaa
     3541 gatgaccaag ttgccttgat ggatcgtatg atcaaggaac aacctgccga agctacaaca
     3601 actgaagaga ctagcagtag tttctttagc caagttgcta aaattctttc tgaaaactgg
     3661 caacaactct tgcgtggtgc tggtatcact cttttaatct ctatcgtcgg aaccatcata
     3721 ggtctcatta ttggacttgc cattggtgtc ttccgtactg ctcctctctc tgaaaacaaa
     3781 gtcatttacg gcctacaaaa actagtcggc tgggttctca atgtctacat tgaaattttc
     3841 cgtggtacgc caatgattgt tcaatcgatg gttatctact atggaactgc ccaagctttc
     3901 gggatcaacc ttgaccgtac actggctgct atcttcatcg tttcaatcaa taccggtgcc
     3961 tacatgactg aaatcgtccg tggtggtatc ctagcagttg acaagggaca atttgaagct
     4021 gcgactgctc ttggtatgac ccataaccag accatgcgta agattgtcct acctcaggta
     4081 gtccgtaaca tcctacctgc aactggtaat gaatttgtca tcaatatcaa agatacatct
     4141 gtattgaacg ttatctctgt tgtcgaactt tatttctcag gaaataccgt ggcaacacaa
     4201 acctatcaat acttccagac atttacaatc atcgccgtga tttactttgt cctcaccttc
     4261 accgtaacac gtatcctacg ctttatcgag cgcagaatgg acatggatac ctacactaca
     4321 ggtgctaacc aaatgcaaac ggaggatttg aaataatgac acaagcaatc cttgaaatta
     4381 aacacctcaa aaaatcctat ggacaaaacg aagtgctaaa agacatttca ctcactgtcc
     4441 acaagggaga ggtcatctct atcatcggaa gctctggaag cggaaaatcg accttcctac
     4501 gctccattaa cctacttgaa acaccaactg atggacaaat cctttatcat ggacaaaacg
     4561 tcctcgaaaa aggctatgac ctcacgcaat accgtgaaaa gttggggatg gttttccaat
     4621 cctttaacct ctttgaaaat ctcaatgttc ttgaaaacac aatcgtcgct cagacaactg
     4681 tcctaaaacg cgaacgcaca gaagctgaaa agattgccaa agaaaacctg gaaaaggtcg
     4741 gcatgggaga acgctactgg caagccaaac caaaacaact ctcaggtggt caaaaacaac
     4801 gtgtggccat cgctcgtgcc ctctccatga atccggacgc tattctcttt gatgaaccaa
     4861 catcagctct cgatccagaa atggttggag aagtcctcaa aatcatgcag gacctggctc
     4921 aggaaggctt gaccatgatt gtcgtaaccc atgaaatgga atttgcccgt gatgtctctc
     4981 accgtgttat ctttatggat aagggcgtga tcgctgaaga aggtaaacca gaagacctct
     5041 tcaccaatcc taaagaagac cgaacaaaag agttccttca acgctatctc aaataaaaag
     5101 aaaaggctgc atcaaccgtg cagtcttttt gctgtcctca aaatgaaaag gcagattctc
     5161 tatctctact acttgaaggt caagaatccg ccgttatcca aagattaatc ttcaaatttt
     5221 tcataatttt tttcatagaa atcaattagg cctagggctg tttcaaatga aatatttttt
     5281 acttttgcac gaccctgcgc cagagcaatg atagacattt cacgcgcatt cgtttcttta
     5341 gagatacggt agcctgtgat tttcttatca cgaacccagc caacaactga ttctactttt
     5401 tcaaagtttg acttagccat gtttttctcc tattttcttc tttataatat ttaattgtat
     5461 acgtttttac tacttttgtc aataaaaaaa acatatattc aacgaaaata attagttcag
     5521 aattttctta cttccttttt aaatccgata atatataggt tttgctagcc tctacaccat
     5581 aaatatttgc taataaaaat actgtattat taccctctta aggtgcgaaa ttattcaagt
     5641 tcttaaaaaa tgcttctcta tttccgtaag atagcttatt tcttcgattt aagaactgaa
     5701 gatgatagtt ctaaccttta gaaatccaag ccttcactag ctcaaaaact cctatctttc
     5761 ttcctcaata aatcatctga gagataggta ttccttatgt ccttcaaatt catcactcct
     5821 ttggatattg aggtgctagc ctagaagcag acacccgatt atctaaacca tccttaatag
     5881 agatgacaag acagataagc tcagttaatt acttatatat ctttttaata tagtatattt
     5941 gtcaacaaaa agaaatcaaa tcatacctta tactcaatga aaatcaaaga gcaaactagg
     6001 aagctagccg caagttgctc aaaacactgt tttgaggttg cagatagagc taacgtggtt
     6061 tgaagagatt ttcgaagagt attaaagaca gtgtgactat gcttgtcaat agataggaaa
     6121 tcaaaatgaa ggtatgatat ttttttatta gtcctcagac aagcatatta taaagcgttt
     6181 cattaccatt taagttaata taagctggat caaaaccttc cattctacga atcaaacctg
     6241 cataatcatg cttatctgct aaagcgatcc caattaatac tgggcctgtt cccttgctag
     6301 ctcgtttgat atactcaaaa cgtgtgatat catcatttgg ccccaggata tcatttacaa
     6361 actcacgcaa agctcctgga cgttgtggga aattgaccac aaagtaatgt ttgataccat
     6421 cataaatcaa ggcacgctct tccatttctg gcatacggtt gatatcatta tttcctccag
     6481 aaatgataca acaaatggtt ttccccttaa tatattcagc taaaacctct aaagaggcga
     6541 tactagccgc tccagcaggt tctgcgacta tcccttgctt agagtaaagg tcaatcaagg
     6601 tttcagaaat caatccctca tcgacaccta ccaaagtttt aatatgttga cgagttgctt
     6661 cataggtcaa ctgacctacc ttttgcacag caatcccatc agcaaattta tcaatttcct
     6721 tgagttttac tggacctcca gcctcaaagg cagctttcat ggaacgcgct ccattcgcct
     6781 ctactccgat aacctcaatc tctggacttg tttccttgat ataggtagaa accccggcaa
     6841 tgagaccgcc accaccaaca ggaaccaaga cagcatcaaa atcaatcgat tcttttcgag
     6901 cttcttctaa aatctcataa gcaactgttc cttgacctgc ttgaacatga gcatcatcaa
     6961 aaggatcaat aaaggtacga ttttcagaga ctgtaaattc ttgagctgct ttggctgagg
     7021 catcaaaggt atctccaact agtttaatag ttacaaaatc cccaccaaaa aagcgaacct
     7081 gaccaatctt ttgttgtggc gtagtaatgg gcataaagat agtagcagga attttcattt
     7141 cattacaagt ataggctact ccctgcgcat gatttcccgc agaagcgcag actaccccac
     7201 gttcacgttc ttccttgctg agctgggaaa tggcataata ggcaccacga attttaaagg
     7261 agcgaacacg ctgggcattt tcttttttca aataaatctt agcaccatac ttctccgata
     7321 aataatgatc gtaatccagt ggagtattca caaccacacc gttcaagacc ttgtgagcct
     7381 tgattatatc ttttgaactt aacatataaa acctcttttt ctataaaaaa cagtcactaa
     7441 tgactgtttt ttagttattc atctccgaaa acttcagtaa cactttcatg gtatttatga
     7501 gccatacgaa tcgaaaactc tttaggaagt tcataacgat ttgtggaaat ccaatcatat
     7561 aaccattcta ccaaactttc ttcactatca ttacgatgga agataaaata gtttaaaatt
     7621 tctttatatt ctacatcatt caaatccaac gtgtctacaa attgactacg aggaacaaaa
     7681 ttagaatcta attttcttag ttgatctgtc aattctccca attgtaaatc caactgaaaa
     7741 aatagttttt gcagttgcct atcagtttca tcacatcctg tcttgataga aaacaaattg
     7801 gtaatattca tatgatttca acccctaaat aagtacatct aatatcccac gaatagctga
     7861 agctaaagtt ttcgcagtcc agtcatttaa acccgattct ttcaatccag atgcaataat
     7921 ttgctctatt tgatataaac cgccatctat cgtctcattt attctgcgaa gaggaccttc
     7981 aatataggat aaagcttctc cgaatctaac agctgattta ggtcctaatg atttaccaac
     8041 tatatttgtt aatgtttttt atttctcaaa atccatttcg ctatataacg agcagattta
     8101 gtcgcaccag ctctctcaaa cggagaatga atttcaagac cttcttctct agctatttgt
     8161 gccaatttga agaattgttc aaagttcata tgcaacctac catcttcaaa attagcatca
     8221 tcataaatgt taattacagc attttcatca tacgaccaat ctgctttatt ttggtctgct
     8281 agcactatta ccgacggtgt aattacatta tacaactaga accaataata acgatgcaat
     8341 ttttctcata atctttttca taaaatcatc tccagcgcta tatatttcta attagttata
     8401 gattttgaat gcatcatcgt cgtttttacc aacgaatggc attgctttac gcaattctgc
     8461 accaactttt tcaatttcaa ggttagctgc ttgttcacgg taagcagtca attttggacg
     8521 tccagcttta tagtcattta caaagtcatt tgcaaattta ccattttgga tgtctgccaa
     8581 gacagccttc atattttctt taacttgttc agtgattaca cgtggacctg atacatagtc
     8641 accgtattca gcagtgtttg aaatagattg acgcattttc ttgaatccac cttcgtagat
     8701 caagtcaacg atcaatttca tttcgtgaag aacttcaaag taagccaatt ctggagcgta
     8761 acctgcttct gtcaagactt cgaaacctgc ttcgataagg gcagtcaaac caccacaaag
     8821 tacagcttgt tcaccaaaca aatcttcttc agtttcttct ttgtaagttg tttcaagaag
     8881 acctacacga gccgctccaa cacctttaca ccagtccata gcaatgtttt tagcatttcc
     8941 tgttgcatct tggtatactg cataaagagc tggaacacca aatccttctt cgtaagtacg
     9001 acgtaccaag tgtcctggtc ctttaggagc acacatgaag acatctacat ccgcaggaac
     9061 tttgataaat tcaaagtgga tgttgaaacc atgggcaaat ccaactgcgt ttccagcttc
     9121 caagtttgga gcgatttctg cttcgtacaa ttcttgttga atttcgtctg gcgccaagat
     9181 catgataaca tcagccaact tagtagcttc tgctactgtg taagtatcaa atccatcttc
     9241 ttttgcttta tcaaaagatt tacctggacg tacaccgata ataacgtcac gacctgaatc
     9301 acgcaagttt tgagcatgcg catgcccttg tgaaccataa ccgataacgg cgattttttt
     9361 accgtcaagt gctgctactt taacatcttt ttcatattcc atttgaactg tcatagcttt
     9421 tctctctttt ctattattta ttgcctttta ggctggttta ataaatttaa gttggatttt
     9481 taatcgcggg taaatccagt tgcacccgtt cgagcaatat tgcgaatacc gtatgggcga
     9541 atgactcgca atagggcttc gctcttttct gcatttcccg tcatctgaat ggtaatcgag
     9601 cttggcgcta cgtctactac tgttgcacgg aaaggttgaa taatcgctaa aatctcagct
     9661 ctcttctcag ctggcgctga catcttaacc aaaatcacct cgcgctccaa atgaggcttg
     9721 tctgtaatat ctcgaatgcg aatcacatca atctgacgat tgagctgttt gatgatttgc
     9781 tccacttcat catgagaagc aacatcaata ataatagtga tacgcgatac attcggatct
     9841 tctgttgctc caacagagat gctttcaata ttaacctgac gacgagatag gacacctgta
     9901 aagcgattga ggactcctga acgattttgt agttttgctg ttaacattct acgcatggaa
     9961 ctgcaccccc aacatctcat gattactctt accagccggt accattggta acacctgttc
    10021 cttacgagaa atatctacct caattagcat aggaacatcc tcagtgatga cttcaaggtc
    10081 ttgagccaag gtctcaggat tgtcaaactt atagttttta ataccataag cctgcgccat
    10141 caattggaaa tcaggaaggg tatcaaagac cgactctgat gttctgcctt catagaagga
    10201 ttcctgccac tggcgaacca ttccaagtga atgattgttc agcataacca ccttgattgg
    10261 caccttgtaa atattcaaaa tagccaactc ctggttggtc atttggaaac caccatcccc
    10321 aacaaacaag actacttcct tatctgggtt agcaatttta gcaccgattg ctgctggaat
    10381 tccaaagccc attgttccca aaccacctga agtcactaac tgacgttcat tttggtaggg
    10441 ataatactga gctgtccaca tttggtgttg accaacgtct gttaccacaa tggcatctcc
    10501 attcgtcaat tcaccaattc gttcaataac tgcttgcggt tgaaccacac gctctttctt
    10561 atcataagaa cgaacacgat tcttgtcttt agtgactttc tcaatccact tttcagtgtt
    10621 gttgtgaact gttggttctg ctagcaacat ttgcaaggcc ttcttagcat ctccaactac
    10681 aggaatgtct gcactgataa tcttgccaat ctcagctggg tcaatatcaa tgtgggcaac
    10741 cttagcattc ttagcgaaag tcttaggatt ccccgtcaaa cggtcatcga aacgagaacc
    10801 aatactaatc ataaagtccg cttccgtcat agcaatattt gctgcgaatg acccgtgcat
    10861 gcctcccatt ccaagaaaga gtgggtgact cgttgcaatc gttccttgtc ccaaaagact
    10921 ggttaccact ggaatttgat agcgttctgc aaattcattt agttccgtag cagcctcagc
    10981 ataactaatt ccaccaccag ctaacaagac tggcttttta gccttggaca attgcttcaa
    11041 gattttcttg atttgcatat cattcggctc aagagtcggc tgataacttg gtaaattcac
    11101 ttctggtgaa taaatgaagt ctgtttctaa agcagatatg tcttttggta ggtcaattac
    11161 aactggccct ggacggcctg tagttgcgat atggacagct tccgtaatga tacgcggaat
    11221 atcagctgtc tcacgaactt ggtaattgta cttagtgatt ggcatggtaa ttcccacgat
    11281 gtctgcctcc tgaaaggcat ccttcccaat ccctgctcgc gccacctgac ctgtaaagac
    11341 caaaagggga acgctatcgc tcatggcatc cgcaatccct gtaatggcat ttgttgctcc
    11401 tggtccacta gtgacgacgg caacacccaa ctttccagtt gatttggcat aaccttcagc
    11461 ttcatgcaaa caaccttgct catggcgccc tagaatgtgg cgaatgcctt taaaattata
    11521 tatcgcatca taaaaaggca aaaccgcacc accaggataa ccaaagatgg tatcaactcc
    11581 taaatcacga agtgtttcca aaactaggtc cgaccccgtc ttaggagatt ctaaactgat
    11641 tttctccatt attccccttt ctttcctctt aaaaataact tgttactatc ataccatttt
    11701 ttcaaaattt ttcaagataa aagaagaaat tttctgaatt ttctatttta acgtttattt
    11761 atgaatgtta tttttgtttt tattaaaaag ataccgattt catttactaa aaagaaaaaa
    11821 gaactgattt ctcagtcctt cattaatctt attccacact aaataggtat gggtaaacag
    11881 gttgttgacc ttggtgaatc tcgacttcaa cgtcttcgaa ttcttctacg atttcttgag
    11941 cgatttcatt ggcaagttct tcgcttccgt cttcacctac atagaaggtt acgatttcac
    12001 tgtcttcatc caacatatgt ttcaaggttt cagtcaatgt ttggtgcata tcagggtttg
    12061 acacaagaat ttttccatcc accataccta aattatcgtt ttcatggatt tctaagccat
    12121 cgatcgttgt atcacgcacg gctgttgtga cgcttccgct aacgacatcg ctaagagcag
    12181 ctgtcatacg ctcttggttt tcttcaatgg acttgcttgg atcaaaggca agaagacttg
    12241 tcataccttg aggaagagtg cgagcctcta ccactaccgc tggttgctcc aaaacttctg
    12301 ccgcagattg agctgccatg aagatgttct tgttgtttgg caagaagatg atgttacggg
    12361 cattaacctg ttcaacagcc ttgataaagt cttctgttga agggttcatg gtttgaccgc
    12421 cttcgataac ataatccacg ccttgagaac agaagatatc tgctagacct ttaccagcca
    12481 ccacagcaat caaagcatac tctttttctt cagccgactt gataacttga gtagcttctt
    12541 tctcaacctg tgcttcgtgt tggttacgca tattgtcaac ttttaccttg accaagctac
    12601 catatttgag accttcttgc ataacaagtc ctggatcttc tgtatgaaca tggactttga
    12661 caatttcatc atcgttaaca acaaggagag aatctccaag ctcatccaag tagttacgga
    12721 attcatcgta gtcaaaatct ttagcatagg ttggaccttg cttaagagct accatgattt
    12781 cagtacagta accaaacgtg atgtcctcag tcgctacgtg accagctaca gacttatgat
    12841 gctctacatt gatcatctca ctcatgttgg caggagtcgc tacaaagtcc tcagatgcaa
    12901 tatattcgcc agtaagggct gaaaggaaac cttcgtagat gaagaccaat ccttgaccac
    12961 ctgagtccac aacgccaact tctttcaata ctggaagcat gtctggtgtt ttagctagag
    13021 ctgttttagc accttccaag gctgcgcgca tgacttcaac agcgtcatct gtttgctcag
    13081 cttttttctt agcaccgata gcagctccac gagaaactgt taaaatcgtt ccttcaacag
    13141 gtttcatcac tgccttatag gcaacttcca cacctgattg gaaggccaga gccaagtctt
    13201 gacctgttaa ctcgtcttta tccttgatag cttgggaaaa tccacggaaa agctgagacg
    13261 taatcactcc tgagttccca cgcgcaccca tcaaaagccc tttggcaaga atgctcgcta
    13321 cttctccaac tgtagaagct ggcttgtctg caacttcttt agcaccattt tcaatggtca
    13381 ttcccatatt tgtcccagta tctccatctg gaactggaaa gacgtttaat gaattgacat
    13441 attcagcttg cttattcaag cgagttgatg cagcctgcac catttcttga aataagctag
    13501 tagtaatttt tgacacggtt attctcctac aactttgata ttttgaatgt agacatttac
    13561 agtctgagca gtaattccaa gctggttttc caagctaaag gcaacacgct cttgaatgtt
    13621 ttttgacact tcactaatct ttgttccgta gcttaacacg gtatatacat caactgcaat
    13681 actgccatct tcggctgcct ttacgacgac acctttagaa taattttcct tacctagcag
    13741 ggcttggaaa ttatctttga gggcattttt actagccata ccgaccacac caaaaatctc
    13801 agttgctgct ccacctacga cggttgcaat cacttcatct gttagttcga tttgaccatc
    13861 ttttgtatta atttttacag tcatcctttt tacctcaact agttgatact ctattttatc
    13921 atatttcagc ccaagtgtaa aagcagaatg atgtatcagc ggatatttac tctatttttc
    13981 aaacgatgtt atacccacaa taaaagaaaa aagaccctaa ggtctccttt gcttttatta
    14041 ttaaacgcgt tcaactttac ctgatttcaa agcacgagct gaagcccaaa cttttttagg
    14101 tttaccatcg ataagaacag taactttttg aaggtttggt tttacggcac gttttgtttg
    14161 gttcatcgcg tgtgaacggt tgtttcctga tacagtctta cgacctgtaa agtaacatac
    14221 tttagccatt gtgttttcct cctattagat ctaatatagc ggatgtgcta gcaccacata
    14281 ccgtactatg ttatcacatt ttcttgtttt ttgcaaggga attggaagat tttttatttg
    14341 tgtcttaaat caggtcttgc gtgacatttt ctgctctcca catgccatcg ttgattaaca
    14401 gaacaccaga attaaaatta tgtgtataaa aatcatctct aactgcagct aagggtatag
    14461 ccgtcaagtc caaatcccac agctcatcta tcgattttct tacaacaata tctgaatcca
    14521 aatacagtac acgagactcg cttacatact ttggaataaa atacctaaaa aagccgcata
    14581 tgaaagtccc tcaaagggga gacgataacc tttcagaata ttactgtcaa tctaaacatt
    14641 cacaatctca ctattcaaag tctctagtct tttttccatc aattggaacc attctcgcgg
    14701 aaggtcatca ttaaaaacat aaaacttaag attataatga tgaacacaaa gagattttat
    14761 tgttgtttca actttatcca tataagcatt atctgcacct aagacaatcg cttttttctc
    14821 ttctttcact ttttatctca tttcttttta ttcccatcat attattccca tcatatgttt
    14881 cccatcatat gtttctacgt aaccattatt ttcgcctatt cgttcgtaaa accataccag
    14941 tggagatttt agatgaagtc ccattacggt ttacaatttt tacattacga cacggagttt
    15001 tacaaatcga tttcatttgc caaacgtagt tagtgaggca gttagctagt tcgccaaata
    15061 gcgactagcg tccaacaatt tggaacttta gttccaattg ttggtactga gtcacatctt
    15121 ctcctctaac tctacgtctg gatacttgtc cgcaaaccag cggagggcaa agtcattttc
    15181 aaagagaaag actggttggt caaaacggtc tttggctaag atattgcgac ttgacgacat
    15241 ccgttcatcc aagtcctcag gcttgatcca acgaacggtc tttttaccca ttgggttcat
    15301 aactacttcc gcattgtact cgccttccat gcggtgttta aagacttcaa actggagttg
    15361 acctacagcg cctagcatgt actcacctgt ttggtaattc ttataaagct gaacggctcc
    15421 ttcttgcacc aattgctcaa tccccttgtg gaaggatttt tgcttcataa cattcttagc
    15481 agaaactttc atgaaaatct caggtgtaaa ggttggcagg ggttcaaatt caaacttgtt
    15541 ttttccaacc gtcaaggtat ccccaacctg ataagtaccg gtatcgtaaa ccccgataat
    15601 atcacctgcc acggcattgg tcacattctc acgactctcc gccataaact gggtaacatt
    15661 agatagttta gcccccttac cagtacgagg gagattgaca ctcatgccgc gctcaaattc
    15721 gccagatacg atacggacaa aggcaatacg gtcacggtga cgagggtcca tgttggcttg
    15781 gattttaaag acaaagcctg agaaatcctt gtcataagga tccacaattt caccgtctgt
    15841 tttcttgtga ccatgtggtt ctggagcaaa cttgaggaag gtttcaagga aggtctgcac
    15901 accaaagttt gtcagggctg aaccgaaaaa gacaggcgtc aattctccag ccagaatagc
    15961 ttcctctgaa aactcattcc cggcttcatt taaaagctca atgtcatcct tgacttgctc
    16021 gtagaaagga ttgctaccaa agagtttgtc cccgtcttct agactggcaa aacgctcatc
    16081 ccctttgtaa agctctaaac gttggttata gaggtcatac aagccctcaa aggctttccc
    16141 catcccgata ggccagttca tagggtagct agcaatgccc aagatttctt ccaattcttg
    16201 caagagatcc aaaggctcac gaccgtcacg gtccagcttg ttcataaagg taaagactgg
    16261 aatgccacga tgtttcacaa cctcaaacaa tttcttggtt tgagcctcga tccccttggc
    16321 agagtccacg accatgaccg cagcatccac cgccatcaag gtacgatagg tatcttctga
    16381 gaagtcctcg tgccctggcg tgtctaagat attcacgcgc ttgccgtcgt agtcaaattg
    16441 cataacagat gaagtaacag aaatcccacg ttgcttctcg atatccatcc agtcagattt
    16501 agcaaaagtc cctgttttct tcccttttac cgtaccagcc tcacgaatct cacccccaaa
    16561 gtagagtaac tgctcagtga tggttgtttt ccccgcgtcc gggtgggaga taatggcaaa
    16621 ggtacgacgt ttcttaattt cttcttgaat attcataagt tctctttctt tgattctcta
    16681 tttttcttgt ttcaatagct gagaatgatt tttacattgg attttaccat tcctttcaac
    16741 actccattat atcggatttt agcatttttt tcaatttcta tttcttttca cttccccctc
    16801 ccttatttat aggaaaatat ggtaaaatag aacagactaa aaatcatcat ttcacgaaag
    16861 gatgcaagat gaaaattacg caagaagagg taacacacgt tgccaatctt tcaaaattaa
    16921 gattctctga agaagaaact gctgcctttg cgaccacctt gtctaagatt gttgacatgg
    16981 ttgaattgct gggcgaagtt gacacaactg gtgtcgcacc tactacgact atggctgacc
    17041 gcaagactgt actccgccct gatgtggccg aagaaggaat agaccgtgat cgcttgttta
    17101 aaaacgtacc tgaaaaagac aactactata tcaaggtgcc agctatccta gacaatggag
    17161 gagatgccta atgactttta acaataaaac tattgaagag ttgcacaatc tccttgtctc
    17221 taaggaaatt tctgcaacag aattgaccca agcaacactt gaaaatatca agtctcgtga
    17281 ggaagccctc aattcatttg tcaccatcgc tgaggagcaa gctcttgttc aagctaaagc
    17341 cattgatgaa gctggaattg atgctgacaa tgtcctttca ggaattccac ttgctgttaa
    17401 ggataacatc tctacagacg gtattctcac aactgctgcc tcaaaaatgc tctacaacta
    17461 tgagccaatc tttgatgcga cagctgttgc caatgcaaaa accaagggca tgattgtcgt
    17521 tggaaagacc aacatggacg aatttgctat gggtggttca ggtgaaactt cacactacgg
    17581 agcaactaaa aacgcttgga accacagcaa ggttcctggt gggtcatcaa gtggttctgc
    17641 cgcagctgta gcctcaggac aagttcgctt gtcacttggt tctgatactg gtggttccat
    17701 ccgccaacct gctgccttca acggaatcgt tggtctcaaa ccaacctacg gaacagtttc
    17761 acgtttcggt ctcattgcct ttggtagctc attagaccag attggacctt ttgctcctac
    17821 tgttaaggaa aatgccctct tgctcaacgc tattgccagc gaagatgcta aagactctac
    17881 ttctgctcct gtccgcatcg ccgactttac ttcaaaaatc ggccaagaca tcaagggtat
    17941 gaaaatcgct ttgcctaagg aatacctagg cgaaggaatt gatccagagg ttaaggaaac
    18001 aatcttaaac gcggccaaac actttgaaaa attgggtgct atcgtcgaag aagtcagcct
    18061 tcctcactct aaatacggtg ttgccgttta ttacatcatc gcttcatcag aagcttcatc
    18121 aaacttgcaa cgcttcgacg gtatccgtta cggctatcgc gcagaagatg caaccaacct
    18181 tgatgaaatc tatgtaaaca gccgaagcca aggttttggt gaagaggtaa aacgtcgtat
    18241 catgctgggt actttcagtc tttcatcagg ttactatgat gcctactaca aaaaggctgg
    18301 tcaagtccgt accctcatca ttcaagattt cgaaaaagtc ttcgcggatt acgatttgat
    18361 tttgggtcca actgctccaa gtgttgccta tgacttggat tctctcaacc atgacccagt
    18421 tgccatgtac ttagccgacc tattgaccat acctgtaaac ttggcaggac tgcctggaat
    18481 ttcgattcct gctggattct ctcaaggtct acctgtcgga ctccaattga ttggtcccaa
    18541 gtactctgag gaaaccattt accaagctgc tgctgctttt gaagcaacaa cagactacca
    18601 caaacaacaa cccgtgattt ttggaggtga caactaatga actttgaaac agtcatcgga
    18661 cttgaagtcc acgtagagct caacaccaat tcaaaaatct tctcacctac ttctgcccac
    18721 tttggaaatg accaaaatgc caacactaac gtgattgact ggtctttccc aggagttcta
    18781 ccagttctca ataaaggggt tgttgatgcc ggtatcaagg ctgctcttgc cctcaacatg
    18841 gacatccaca aaaagatgca ctttgaccgc aagaactact tctatcctga taaccccaaa
    18901 gcctaccaaa tttctcagtt tgatgaacca atcggatata atggctggat tgaagtcaaa
    18961 ctagaagacg gtacgaccaa gaaaatcggt atcgaacgtg cccacctaga ggaagacgct
    19021 ggtaaaaaca cccatggtac agatggctac tcttatgttg acctcaaccg ccaaggggtt
    19081 cccttgattg agattgtatc tgaggcagat atgcgttctc ctgaagaagc ctatgcttat
    19141 ctgacagccc tcaaggaagt tatccagtac gctggcattt ctgacgttaa gatggaggaa
    19201 ggttcgatgc gtgtggatgc caacatctcc cttcgtcctt atggtcaaga gaaattcggt
    19261 accaagactg aattgaagaa cctcaactcc ttctcaaacg ttcgtaaagg tcttgaatac
    19321 gaagtccaac gccaggctga aattcttcgc tcaggtggtc aaatccgcca agaaacacgc
    19381 cgttacgatg aagcgaataa agcaaccatc ctcatgcgtg tcaaggaagg ggctgctgac
    19441 taccgctact tcccagaacc agacctaccc ctctttgaaa tttctgacga gtggattgag
    19501 gaaatgcgga ctgagttgcc agagtttcca aaagaacgtc gtgcgcgtta tgtatctgac
    19561 cttggtttat cagactacga tgctagtcag ttgactgcta ataaagtcac ttctgacttc
    19621 tttgaaaaag ctgttgccct aggtggtgat gccaaacaag tctctaactg gctccaaggg
    19681 gaagtcgctc agttcttgaa tgctgaaggt aaaacactgg aacaaatcga attgacacca
    19741 gaaaacttgg ttgaaatgat tgccatcatc gaagacggta ctatttcatc taagattgcc
    19801 aagaaagtct ttgtccatct agctaaaaat ggcggtggcg cgcgtgaata cgtggaaaaa
    19861 gcaggtatgg ttcaaatttc agatccagct atcttgatcc caatcatcca ccaagtcttt
    19921 gccgataacg aagctgctgt tgccgacttc aagtcaggca aacgtaacgc cgacaaggcc
    19981 tttacaggat tccttatgaa ggcaaccaaa ggccaagcca acccacaagt tgcccttaaa
    20041 ctacttgcac aggaattggc gaagttgaaa gaaaactaga cagaacaaaa ccagccctaa
    20101 ggttggtttt ttcttctcta ccaactccca ataactattt tggctttatt tccagagtat
    20161 tttatggtaa aatgaagagt aataatattt attaaagagg taaaaacatg attgaagcaa
    20221 gtaaattaaa agctggtatg acctttgaaa cagctgacgg caaattgatt cgcgttttgg
    20281 aagctagtca ccacaaacca ggtaaaggaa acacgatcat gcgtatgaaa ttgcgtgatg
    20341 tccgtactgg ttctacattt gacacaagct accgtccaga ggaaaaattt gaacaagcta
    20401 ttatcgagac tgtcccagct caatacttgt acaaaatgga tgacacagca tacttcatga
    20461 atacagaaac ttatgaccaa tacgaaatcc ctgtagtcaa tgttgaaaac gaattgcttt
    20521 acatccttga aaactctgat gtgaaaatcc aattctacgg aactgaagtg atcggtgtca
    20581 ccgttcctac tactgttgag ttgacagttg ctgaaactca accatctatc aaaggtgcta
    20641 ctgttacagg ttctggtaaa ccagcaacga tggaaactgg acttgtcgta aacgttccag
    20701 acttcatcga agcaggacaa aaactcgtta tcaacactgc agaaggaact tacgtttctc
    20761 gtgcctaatc tctagaaaga ggtcattcta tgggaattga agaacaactt ggcgaaatcg
    20821 ttatcgcccc acgtgtactt gaaaaaatca ttgctatcgc tactgcaaag gtagagggtg
    20881 ttcactcttt ttcaaacaga tcagtgtctg ataccctttc aaaactttca ctcggccgtg
    20941 gcatttatct taaaaacgtg gacgaagaac tcacagcaga tatctatctc taccttgagt
    21001 acggagtaaa agttcctaag gtagcggttg ctatccagaa agctgtcaaa gatgccgtcc
    21061 gtaatatggc tgatgtagaa ctcgctgcta tcaatattca cgttgcaggt atcgtcccag
    21121 ataaaacacc aaaaccagaa ttgaaagatc tatttgacga ggacttcctc aatgactagt
    21181 ccactattag aatctagacg ccaactccgt aaatgcgctt ttcaagctct catgagcctt
    21241 gagttcggta cggatgtcga aactgcttgt cgtttcgcct atactcatga tcgtgaagat
    21301 acggatgtac aacttccagc ctttttgata gacctcgttt ctggtgttca agctaaaaag
    21361 gaagaactag ataagcaaat cactcagcat ttaaaagcag gttggaccat tgaacgctta
    21421 acgctcgtgg agagaaacct ccttcgcttg ggagtctttg aaatcacttc atttgacact
    21481 cctcagctgg ttgctgttaa tgaagctatc gagcttgcaa aggacttctc cgatcaaaaa
    21541 tctgcccgtt ttatcaatgg actgctcagc cagtttgtaa cagaagaaca ataaggctct
    21601 ttgtcaactg tagtgggttg aaaaaaagct aagctcgaga aaggacaaat ttcgtccttt
    21661 cttttttgat gttcaaagcg ataaaaatcc gttttttgaa gttttcaaag tttcgaaaac
    21721 caaaggcatt gcgcttgata agtttgatga gattattggt cgcttccagt ttggcattag
    21781 aatagtgtag ttgaagggcg ttgacaatct tttctttatc tttgaggaag gttttaaaga
    21841 cagtctgaaa aataggatga gcctgcttaa gattgtcctc aataagtccg aaaaatttct
    21901 ctggttcctt attctggaag tgaaacagca agagctgata gagctgatag tggtgtttca
    21961 agtcttgtga atggctcaaa agcttgtcta aaatctcttt attggttaag tgcatacgaa
    22021 aagtaggacg ataaaatcgc ttatcactca gtctacggct atcctgttga atgagtttcc
    22081 agtagcgctt gatatccttg tattcatggg attttcgatg aaactgattc atgatttgga
    22141 cacgcacacg actcatggca cggctaagat gttgtacaat gtgaaagcga tcaagaacga
    22201 ttttagcatt cgggagtgaa acagtctggg agactgtttc agcctgagcc taggaatttg
    22261 aaagcgaagc tgtttagcca agtcatagta agggctaaac atatccatag taataatttt
    22321 gacgcgacat cggacaactc tatcgtagcg aagaaagtga tttcgaatga tagcttgtgt
    22381 tctaccctca agaacagtga tgatattgag attgttaaaa tcttgcgcaa tgaagctcat
    22441 ctttcccttt gtaaaagcat actcatccca agacataatc tcaggaagac aagaaaaatc
    22501 atgtttaaag tgaaaatcat tgagcttacg aataacagtt gaagttgaga tggaaagctg
    22561 atgggcaata tcagtcatag aaatcttttc aatcaacttt tgagcaatct tttggttgat
    22621 gatacgaggg atttggtgat ttttcttgac gagggaagtc tcagcgaccg ctatttttga
    22681 gcactgatag cacttaaagc gacgctttct aaggaggatt ctag
//
LOCUS       DAVKNL010000030        21628 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVKNL010000030 DAVKNL010000000
VERSION     DAVKNL010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21628
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.030"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            <1..221
                     /locus_tag="VW411_001193"
     CDS             <1..221
                     /locus_tag="VW411_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9793922.1"
                     /translation="SQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYWTF
                     LSGKAEKQGITLYHHVLIDLFISYFNPL"
     gene            complement(418..1338)
                     /gene="cysK"
                     /locus_tag="VW411_001194"
     CDS             complement(418..1338)
                     /gene="cysK"
                     /locus_tag="VW411_001194"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEU9793923.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKPGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(1587..2222)
                     /locus_tag="VW411_001195"
     CDS             complement(1587..2222)
                     /locus_tag="VW411_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEU9793924.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHGLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            2278..3576
                     /locus_tag="VW411_001196"
     CDS             2278..3576
                     /locus_tag="VW411_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9793925.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQVVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPML
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            3573..4235
                     /locus_tag="VW411_001197"
     CDS             3573..4235
                     /locus_tag="VW411_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEU9793926.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            4315..4863
                     /gene="raiA"
                     /locus_tag="VW411_001198"
     CDS             4315..4863
                     /gene="raiA"
                     /locus_tag="VW411_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEU9793927.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNIVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            4999..6972
                     /locus_tag="VW411_001199"
     CDS             4999..6972
                     /locus_tag="VW411_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEU9793928.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDETSESDISQINSFVANFISEFSEKHMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDMKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEMKEKEKEE"
     gene            6969..7421
                     /gene="rplI"
                     /locus_tag="VW411_001200"
     CDS             6969..7421
                     /gene="rplI"
                     /locus_tag="VW411_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU9793929.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            7465..8817
                     /gene="dnaB"
                     /locus_tag="VW411_001201"
     CDS             7465..8817
                     /gene="dnaB"
                     /locus_tag="VW411_001201"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU9793930.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            8819..9085
                     /locus_tag="VW411_001202"
     CDS             8819..9085
                     /locus_tag="VW411_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793931.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            9174..10520
                     /gene="cbpD"
                     /locus_tag="VW411_001203"
     CDS             9174..10520
                     /gene="cbpD"
                     /locus_tag="VW411_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEU9793932.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRV
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLASGTVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSATVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSHSSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGLWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      10603..10705
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10776..11066
                     /locus_tag="VW411_001204"
     CDS             10776..11066
                     /locus_tag="VW411_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEU9793933.1"
                     /translation="MKASIALQVLPLVQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            11029..11787
                     /locus_tag="VW411_001205"
     CDS             11029..11787
                     /locus_tag="VW411_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9793934.1"
                     /translation="MRNLRSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     KPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRS"
     gene            11826..12833
                     /locus_tag="VW411_001206"
     CDS             11826..12833
                     /locus_tag="VW411_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9793935.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            12833..13561
                     /locus_tag="VW411_001207"
     CDS             12833..13561
                     /locus_tag="VW411_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9793936.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            13680..14138
                     /locus_tag="VW411_001208"
     CDS             13680..14138
                     /locus_tag="VW411_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEU9793937.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQIIQEVDRKGK"
     gene            14140..16572
                     /locus_tag="VW411_001209"
     CDS             14140..16572
                     /locus_tag="VW411_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9793938.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDSNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLTE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            17214..17867
                     /gene="rr06"
                     /locus_tag="VW411_001210"
     CDS             17214..17867
                     /gene="rr06"
                     /locus_tag="VW411_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEU9793939.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDLWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            17864..19195
                     /gene="hk06"
                     /locus_tag="VW411_001211"
     CDS             17864..19195
                     /gene="hk06"
                     /locus_tag="VW411_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEU9793940.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIYE
                     LESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSI
                     NKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIENQLT
                     DATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQELEH
                     LFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            19278..19805
                     /locus_tag="VW411_001212"
     CDS             19278..19805
                     /locus_tag="VW411_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEU9793941.1"
                     /translation="MVLAIILVTFFIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYVTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            19884..>21628
                     /gene="cbpA"
                     /locus_tag="VW411_001213"
     CDS             19884..>21628
                     /gene="cbpA"
                     /locus_tag="VW411_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpA"
                     /protein_id="HEU9793942.1"
                     /translation="MFASKSERKVHYSIRKFSVGVASVVVASLVMGSVVHATENEGAT
                     QVPTSSNRANESQAEQGEQPKKLDSERDKARKEVEEYVKKIVGESYAKSTKKRHTITV
                     ALVNELNNIKNEYLNKIVESTSESQLQILMMESRSKVDEAVSKFEKDSSSSSSSDSST
                     KPEASDTAKPNKPTEPGEKVAEAKKKVEEAEKKAKDQKEEDRRNYPTITYKTLELEIA
                     ESDVEVKKAELELVKVKANEPRDEQKIKQAEAEVESKQAEATRLKKIKTDREEAEEEA
                     KRRADAKEQGKPKGRAKRGVPGELATPDKKENDAKSSDSSVGEETLPSPSLKPEKKVA
                     EAEKKVEEAKKKAEDQKEEDRRNYPTNTYKTLELEIAESDVEVKKAELELVKEEAKEP
                     RNEEKVKQAKAEVESKKAEATRLEKIKTDRKKAEEEAKRKAAEEDKVKEKPAEQPQPA
                     PAPKAEKPAPAPKPENPAEQPKAEKPADQQAEEDYARRSEEEYNRLTQQQPPKTEKPA
                     QPSTPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNSNGAMATGWLQNNGSWYY
                     LNANGSMATGWLQNNG"
BASE COUNT         6826 a         3588 c         4925 g         6289 t
ORIGIN      
        1 cgagtcaata ctatttggag cccctttata acctcttgcg agagagacta ttgactcagc
       61 ccttacttca tgcggatgaa acttcttata gggtgctaga gagtgatagt cagctgactt
      121 actattggac ttttttgtct gggaaagctg agaaacaagg gattacgctt taccatcatg
      181 ttctaatcga tttgttcata tcttatttca atccattata aatagtgaga aatatctatc
      241 ctatcttcta gaatgtcttc caaacgtgga aactctcgta aacaaagagg ttttagaggc
      301 ctatttacca tggactaaag ttgtacaaga aaagtgcaaa taagaaatct ccagattagg
      361 aactatccgt gagttctcta gtctggagat ttttcaatag acttcgttat tggacggtta
      421 caattcataa agtgctgtag agagataacg ttcaccgtta tctggtgcta gggcaaggac
      481 ttttttacct gtacctaatt ttttggcaac ctcgatggct ccgtagatag ctgcagctga
      541 ggaaatccct acaaggaagc cttcttttcc accaatttca cgtccgagtg caagagcgtc
      601 atctgatgtt acacgaacga taccatcata ggctttagta tcaagtgtat caggaataaa
      661 tccagctgag ataccttgaa ttttgtgagg accaggtttt tcaccagata gaatagcaga
      721 ttcatctgct tctactgcaa aaacttgaat gttagaattt tctgatttga gtgcatgaga
      781 aacaccagaa atcgttccac cagtacctac tccagcaaca aaggcatcta atccatcttt
      841 accgaaagca gctagtatct cagctcctgt tgttctttcg tgtacttctg gattagctgg
      901 attgtcaaat tgaagaggaa ggaaaccatc acgttcagca gcgatttctt gagccttagc
      961 aatagcacct ttcattccct cgctaccagg agttaggacg agttcagcac cataagcttg
     1021 gataatttta cgtcgttcta cactcatagt ttcaggcata acgatgacga ctttataccc
     1081 tttagcagca cctacccatg aaagtccaat accggtgttt ccacttgttg cttcaacaat
     1141 agtagaacca ggtttcagaa taccatcttg ttcagctttt tcaatcatgc taagggcaat
     1201 acggtctttt acagatgaac caggattaaa tgcttcaagc tttatataga cgtctgcagc
     1261 accttctggc acgatgttgt taagtttaac aatcggtgtt tgaccgatta attcagtaat
     1321 gttgttataa atagacatat gaatacctct ttgtataaga tatctttagt ataacgctat
     1381 ccataccatt tctatatatc atagatcaaa gtcattaatt ttcaagtgat tgcgttcagc
     1441 taattctagt aaataggtgt gaaggaaaac gatatcaggt ttcgtttgaa ttgcttcatt
     1501 ttctagtata ttttggtacg cctcttttta ttttgctcgc taaagtagtt gtgagaatgt
     1561 caacgaaacg ctgtattatt cattgtttac actaagttta caggaacttc aacctctcgt
     1621 aaaccttggt cagttaaagt gacttttcca ttaaaaaact ccacaagtgc agctttaata
     1681 gtttcttttt cttctttatc aacataaatc atcgtatcga cttgatctgt aaagtttgta
     1741 tccagctcca tgagaccatg ttctttaagg aagttactgt actcttggta ctgagcataa
     1801 gacatttgaa tagcaatgcc agcctgttct tttatttcaa taataccaat ttctttgaca
     1861 gctaaggcga cactgccggc gtaagcacga attagtcctc cagcgcctag tttaatacca
     1921 ccaaagtagc gtgtcacgac cacacagaca ttggtgagat tgtgattttc tagtacccca
     1981 agcatgggaa caccagcagt accactaggc tcaccatcat cacttgtacg tttaatttca
     2041 ctacgttctc caataatgaa ggcagagcag ttatgtgtcg ctttgtagtg ttcttttttg
     2101 atggcagtaa tgaagtcacg agcctcttct tcgctataaa cacgcttggc atggcagata
     2161 aagcgagatt ttttgatttc ttcttggact tgaccgtcct ctttaattgt cctaaattcc
     2221 atgatttaat tgtactaaaa aatcatctaa agtgctagtt tttacgaata aagaagtatg
     2281 aaagtaaatt tagattatct cggtcgttta tttactgaga atgaattaac agaagaagaa
     2341 cgtcagttgg cggagaaact tccagcaatg agaaaggaga aggggaaact tttctgtcaa
     2401 cgctgtaata gtactattct agaagaatgg tatttgccca tcggtgctta ctattgtcga
     2461 gagtgcttgc tgatgaagcg agtcagaagt gatcaaactt tatactattt tccgcaggag
     2521 gattttccaa agcaagatgt tctcaaatgg cgcggccaat taactccttt tcaagagaag
     2581 gtgtcagagg gattgcttca agtagtagac aagcaaaagc caaccttagt tcatgcggta
     2641 acaggagctg gaaagacaga aatgatttat caagtagtgg ctaaagtgat caatgcgggt
     2701 ggtgcagtgt gtttggctag tcctcgcata gatgtttgtt tggagctgta caagcgcctg
     2761 caacaggatt tttcttgcgg gatagctttg ctacatggag aatcggaacc ttattttcga
     2821 acaccactag ttgttgcaac aacccatcag ttattgaagt tttatcaagc ttttgatttg
     2881 ctgatagtgg atgaagtaga tgcttttcct tatgttgata atcccatgct ttaccacgct
     2941 gtcaagaata gtgtaaagga gaatggattg agaatctttt taacagcgac ttcgaccaat
     3001 gagttagata aaaaggtccg tttaggagaa ctaaaaagac tgaatttacc gagacggttt
     3061 catggaaatc cgttgattat tccaaaacca atttggttat cggattttaa tcgctactta
     3121 gacaagaatc gtttgtcacc aaagttaaag tcctatattg agaagcagag aaagacagct
     3181 tatccgttac tcatttttgc ttcagaaatt aagaaagggg agcagttagc agaaatctta
     3241 caggagcaat ttccaaatga gaaaattggc tttgtatctt ctgtaacaga ggatcgatta
     3301 gagcaagtac aagcttttcg agatggagaa ctgacaatac ttatcagtac gacaatcttg
     3361 gagcgcggag ttaccttccc ttgtgtggat gttttcgtag tagaggccaa tcatcgtttg
     3421 tttaccaagt ctagtttgat tcagattggt ggacgagttg gacgaagcat ggatagaccg
     3481 acaggagatt tgcttttctt ccatgatggg ttaaatgctt caatcaagaa ggcgattaag
     3541 gaaattcaga tgatgaataa ggaggctggt ctatgaagtg cttgttatgt gggcagacta
     3601 tgaagactgt tttaactttt agtagtctct tacttctgag gaatgatgac tcttgtcttt
     3661 gttcagactg tgattctact tttgaaagaa ttggggaaga gaactgtcca aattgtatga
     3721 aaacagagtt gtcaacaaag tgtcaagatt gtcaactttg gtgtaaagag ggagttgaag
     3781 tcagtcatag agcgattttt acttacaatc aagctatgaa ggattttttc agtcggtata
     3841 agtttgatgg agacttcctg ttaagaaaag ttttcgcttc atttttaagt gaggagttga
     3901 aaaagtacaa agagtatcaa tttgttgtaa ttcccctaag tcctgataga tatgctaata
     3961 gaggatttaa tcaggttgag ggcttggtag aggcagcagg ctttgagtat ctggatttat
     4021 tagagaaaag agaagagaga gccagttctt ctaaaaatcg ttcagagcgc ttggggacag
     4081 aacttccttt ctttattaaa agtggagtca ctattcctaa aaaaatccta cttatagatg
     4141 atatctatac tacaggagca actataaatc gtgttaagaa actgttggaa gaagctggtg
     4201 ctaaggatgt aaaaacattt tcccttgtaa gatgaggaaa aaatttgcaa aaatagaatg
     4261 aaagcgttat aataaaatca taaaaacaaa aatgtttaga aagaaggtac tcatatgatt
     4321 aaatatagta tccgtggtga aaacctagaa gtaacagaag caattcgtga ttatgtagtt
     4381 tctaaactcg aaaagatcga aaagtacttc caaccagaac aagagttgga tgcccgaatt
     4441 aacttaaaag tttatcgtga aaaaacggct aaagtggaag taacgattcc gcttggatct
     4501 attactctcc gcgcagaaga tgtatctcaa gatatgtatg gttcaattga ccttgtaact
     4561 gataaaattg aacgtcagat tcgtaaaaat aaaacaaaaa tcgagcgtaa aaataaaaat
     4621 aaggtagcaa ctggtcaatt atttacagat gctttggtgg aagattcaaa tattgtccag
     4681 tctaaagttg ttcgttcaaa acaaattgat ttaaaaccaa tggatttgga agaagcaatt
     4741 ctacaaatgg atttattggg gcatgatttc tttatctatg tggatgttga agatcagaca
     4801 accaatgtga tttatcgtcg tgaggatggc gaaattggtt tgttagaggt taaagaatct
     4861 taatttcaat atgtgtaaag gtaggtttac tgaattgtaa actacctttt tcttataatt
     4921 gatagaatta ctgattacac ttttaaaaag tcgctttttg aggggtatta tggtataatg
     4981 ggtgccaaga ggttttgaat gaaaaaattt tatgtaagtc caatttttcc tattctagta
     5041 ggattgattg cgtttggagt cttatccact ttcattattt ttgttaataa taatctgttg
     5101 acggttttaa ttttgtttct ttttgtagga ggctatgttt ttttatttaa gaaactgaga
     5161 gtgcattata caaggagtga tgtagaacag atacagtatg taaaccacca agcggaagaa
     5221 agtttgacag ctctattgga acagatgcct gtaggtgtta tgaaattgaa tttatcttct
     5281 ggagaggttg agtggtttaa tccctatgct gaattgattt tgaccaagga agatggtgat
     5341 tttgatttag aagctgttca aacgattatc aaggcttcag taggaaatcc gtctacttat
     5401 gccaagcttg gtgagaagcg ttatgctgtt catatggatg cttcttccgg tgttttgtat
     5461 tttgtagatg tatccaggga acaagccata acagatgaat tggtaacaag tagaccagtg
     5521 attgggattg tctctgtgga taattatgat gatttggagg atgaaacttc tgagtcagat
     5581 attagtcaaa tcaatagttt tgtagctaat tttatatcag agttttcaga aaaacacatg
     5641 atgttttctc gtcgggtaag tatggatcga ttttatctat ttactgacta cacggtgctt
     5701 gagggcttga tgaatgataa attttctgtt attgatgctt tcagagaaga gtcgaaacag
     5761 agacagttgc ccttgacctt aagtatggga ttttcttatg gcgatggaaa tcatgatgag
     5821 atagggaaag ttgctttgct caatttgaac ttggctgaag tacgtggtgg cgaccaggtg
     5881 gttgttaagg aaaacgacga aacgaaaaat ccagtttatt ttggtggtgg gtctgctgct
     5941 tcaatcaagc gtacacggac tcgtacgcgc gctatgatga cagctatttc agataagatt
     6001 cggagtgtag atcaggtttt tgtagtcggt cacaaaaatt tagacatgga tgctttgggc
     6061 tctgctgtag gtatgcagtt gttcgccagc aatgtgattg aaaatagcta tgctctttat
     6121 gatgaagaac aaatgtctcc agatattgaa cgagctgttt cattcataga aaaagaagga
     6181 gttacgaagt tgttgtctgt taaggatgca atggggatgg tgaccaatcg ttctttgttg
     6241 attcttgtag accattcaaa gacagcctta acattatcaa aagaatttta tgatttattt
     6301 acccaaacca ttgttattga ccaccataga agggatcagg attttccaga taatgcggtt
     6361 attacttata tcgaaagtgg tgcaagtagt gccagtgagt tggtaacgga attgattcag
     6421 ttccagaatt ctaagaaaaa tcgtttgagt cgtatgcaag caagtgtctt gatggctggt
     6481 atgatgttgg atactaaaaa tttcacctcg cgagtaacta gtcggacatt tgatgttgct
     6541 agctatctca gaacgcgcgg aagtgatagt attgctatcc aggaaatcgc tgcgacagat
     6601 tttgaagaat atcgtgaggt caatgaactt attttacagg ggcgtaaatt aggttcagat
     6661 gtactaatag cagaggctaa ggacatgaaa tgctatgata cagttgttat tagtaaggca
     6721 gcagatgcca tgttagccat gtcaggtatt gaagcgagtt ttgttcttgc gaagaataca
     6781 caaggattta tctctatctc agctcgaagt cgtagtaaac tgaatgtaca acggattatg
     6841 gaagagttag gcggtggagg ccactttaat ttggcagcag ctcaaattaa agatgtaacc
     6901 ttgtcagaag caggtgaaaa actgacagaa attgtattaa atgaaatgaa ggaaaaggag
     6961 aaagaagaat gaaagtaatc tttttagcag atgttaaagg aaaaggtaaa aaaggcgaaa
     7021 ttaaggaagt accaacaggg tatgcgcaaa actttcttat caaaaagaat ctagccaaag
     7081 aagcgactgc tcaagctgta ggtgaacttc gtggtaaaca aaaatcggaa gaaaaagctc
     7141 acgctgagat gattgcagaa ggaaaagcaa ttaaagcaca acttgaagca gaagaaactg
     7201 ttgtagaatt tgttgaaaaa gttggtccag atggtcgtac ctttggttct attaccaata
     7261 agaagattgc agaagaattg caaaagcaat ttggaattaa gattgataaa cgtcatattc
     7321 aagtacaagc tccgattcga gcggttggtt tgattgatgt gccagtgaaa atctatcaag
     7381 atatcacaag tgtaatcaat cttcgtgtga aagaaggata agtttacacc ttcttgacaa
     7441 gattgtaaaa ggaagggaag tctgatggca gaagtagaag agttacgagt acaacctcaa
     7501 gatatcttag ctgagcaatc cgttttaggg gctatcttta ttgatgagag taaacttgtt
     7561 tttgtgcgag aatacattga gtctcgggac ttttttaagt atgcccatcg tttgattttc
     7621 caagccatgg tcgatttatc cgatcgtggt gatgccatag atgcaacaac ggttcgtact
     7681 atccttgata atcaaggtga tttacagaat attggtggct tgtcttactt ggttgagatt
     7741 gttaattctg tgccaacttc tgctaatgcg gagtattatg ctaagattgt tgcagaaaaa
     7801 gcaatgctac gtcgtttaat tgccaagttg acagagtctg tcaaccaagc ttacgaagcg
     7861 tcacaaccag ctgatgaaat tattgctcag gcagaaaaag ggttgattga tgtcagtgaa
     7921 aatgcaaatc gaagcgggtt taagaacatt cgagatgtgt tgaatctcaa ctttggaaat
     7981 ctggaagctc gctcgcaaca aacgaccgat attacaggta ttgcgacagg ttatcgtgat
     8041 ttggatcata tgacaacagg acttcatgag gaggagttga ttatcttagc agctcgtcca
     8101 gcagttggta agacagcatt tgccttgaat atcgctcaga acattgggac taagttggac
     8161 aaaacggttg ctattttttc actcgaaatg ggtgcggaaa gcttggtaga tcgtatgtta
     8221 gctgcagaag gcttagtgga gtcacattct atccgtacag ggcaattgac agatgaggag
     8281 tggcaaaaat atactattgc tcagggtaat ctagctaacg ccagtatcta tatcgatgat
     8341 acgccaggta ttcggattac agagattcgt tctcgttctc gtaaattggc tcaagaaact
     8401 ggaaatcttg gtttgatttt gatagactat ttgcaactta tcacgggaac tggtcgagaa
     8461 aatcgtcaac aagaagtttc tgaaatttct cgtcagttga aaatactagc caaggaattg
     8521 aaggttccag taatcgctct gagtcagctt tctcgtggtg tagaacaacg tcaggacaag
     8581 agaccggtct tgtctgatat tcgtgaatct gggtctattg agcaggacgc tgatatcgta
     8641 gcttttctct atcgcgatga ctactatgaa cgtggtggtg aagaagagga gggtatccca
     8701 aataataagg tggaagttat tatcgagaaa aaccgtagtg gagctcgtgg aacagtggaa
     8761 ttgattttcc aaaaagaata caataaattt tcaagtatct caaagaggga ggcataagat
     8821 gtcagatgca tttacagatg tagccaagat gaaaaaaatc aaagaagaaa tcaaggcaca
     8881 tgagggacaa gtcgtagaaa tgactttgga gaatggtcgt aagcgccaaa aaaatagatt
     8941 gggtaagcta attgaagttt atccatctct atttattgtg gagtttgggg atgtggaagg
     9001 agataaacaa gttaatgttt acgttgaatc ctttacttac tcagatattc ttacagaaaa
     9061 gaatttgatt cattatcttg actaaagtga gaaattttct cactttttct tttttctccg
     9121 aataatttag gtgaaggcaa tcatcgcttt atattatttt tcaaggagga agaatgaaaa
     9181 ttttaccgtt tatagcaaga ggaacaagtt attacttgaa gatgtcagtt aaaaagcttg
     9241 ttcctttttt agtagtagga ttgatgctag cagctggtga tagtgtctat gcctattcca
     9301 gaggaaatgg atcgattgcg cgtggggatg attatcctgc ttattataaa aatgggagcc
     9361 aggagattga tcagtggcgc atgtattctc gtcagtgtac ttcttttgta gcctttcgtt
     9421 tgagtaatgt caatggtttt gaaattccgg cagcttatgg aaatgcgaat gaatggggac
     9481 atcgtgctcg tcgggaaggt tatcgtgtag ataatacacc gacgattggt tccattactt
     9541 ggtctactgc aggaacttat ggtcatgttg cctgggtgtc aaatgtaatg ggagatcaga
     9601 ttgagattga ggaatataac tatggttata cagaatccta taataaacga gttataaaag
     9661 caaacacgat gacaggattt attcatttta aagatttgga tggtggcagt gttgggaata
     9721 gtcaatcctc aacttcaaca ggcggaactc attattttaa gaccaagtct gctattaaaa
     9781 ctgaacctct agctagcgga actgtgattg attactatta tcctggggag aaggttcatt
     9841 atgatcagat acttgaaaaa gacggctata agtggttgag ttatactgcc tataatggaa
     9901 gctatcgtta tgttcaattg gaggctgtga ataaaaatcc tctaggtaat tctgttcttt
     9961 cttcaacagg tggaactcat tattttaaga ccaagtctgc tatcaaaact gaacccctag
    10021 ttagtgcaac tgtgattgat tactattatc ctggagagaa ggttcattat gatcaaattc
    10081 tcgaaaaaga cggctacaag tggttgagtt atacggctta taacggaagt cgtcgctata
    10141 tacagctaga gggagtgact tcttcacaaa attatcagaa tcaatcagga aacatctcta
    10201 gctatggatc ccatagtagt tcaactgtcg gttggaagaa aataaatggt agttggtatc
    10261 atttcaaatc aaatggttct aaatcaacag gatggctgaa agacggttct agctggtatt
    10321 atttgaaatt atctggtgaa atgcagacag gatggttaaa ggaaaatggt ttgtggtatt
    10381 atctgggtag ttcaggggca atgaaaacag gctggtacca ggtctctggt aagtggtatt
    10441 attcttactc ttcaggcgcc ttagctgtta atacgacggt ggatggctac agagtaaaca
    10501 gtgatggaga acgagtatag aaaattggag taggagaaat ttcctgctct tttctgtaag
    10561 cagtttcctt gacatttttc ttattttgcg ctatcatata tacatataat atatgggagt
    10621 ctgtgtacag tctgagagga agtgttaaac ttcgaccgca cctgatctgg gtaatgccag
    10681 cggagggaac gatacttagt ctaattttgc accttttcca tgtatggtaa aggtttttct
    10741 ttttttaaaa aggaaaacga gaagaggagg ttcttatgaa agcaagcatt gccttgcaag
    10801 ttttacccct agtacagggg attgatcgga tagctgttat tgatcaggtc attgcttatc
    10861 tgcaaactca agaagtgacg atggtagtga caccatttga aacggtcttg gaaggggagt
    10921 ttgatgagct tatgcgcatt ctaaaagaag cgctggaagt ggcagggcag gaggcagaca
    10981 atgtctttgc caatgtcaaa ataaatgtag gagagatttt aagtattgat gagaaacttg
    11041 agaagtatac tgagacgaca cattagtcta ttgggctttc tcggagtatt gtcaatctgg
    11101 cagttagcag gttttcttaa acttctcccc aagtttatcc tgccgacacc tcttgaaatt
    11161 ctccagccct ttgttcgtga cagagaattt ctctggcacc atagctgggc gaccttgaga
    11221 gtggctttac tggggctgat tttgggagtt ttgattgcct gtcttatggc tgtgctcatg
    11281 gatagtttga cttggctcaa tgacctgatt taccctatga tggtggtcat tcagaccatt
    11341 ccgaccattg ccatagctcc tatcctggtc ttgtggctag gttatgggat tttgcccaag
    11401 attgtcttga ttatcttaac gacaaccttt cccatcatcg ttagtatttt ggacggtttt
    11461 aggcattgcg acaaggatat gctgaccttg tttagtctga tgcgggccaa gccttggcaa
    11521 atcctgtggc attttaaaat cccagttagc ctgccttact tttatgcagg tctgagggtc
    11581 agtgtctcct acgcctttat cacaactgtg gtatctgagt ggttgggagg ttttgaaggt
    11641 cttggtgttt atatgattca gtctaaaaaa ctgtttcagt atgataccat gtttgccatt
    11701 attattctgg tgtcgattat cagtcttttg ggtatgaagc tggtcgatat cagtgaaaaa
    11761 tatgtgatta aatggaaacg ttcgtagaat tagaatgttt ctgaaaaaga aaagaggaaa
    11821 tcaaaatgaa gaaaacatgg aaagtgtttt taacgcttgt aacagctctt gtagctgttg
    11881 tgcttgtggc ctgtggtcaa ggaactgctt ctaaagacaa caaagaggca gaacttaaga
    11941 aggttgactt tatcctagac tggacaccaa ataccaacca cacagggctt tatgttgcca
    12001 aggaaaaagg ttatttcaaa gaagctggag tggatgttga tttgaaattg ccaccagaag
    12061 aaagttcttc tgacttggtt atcaacggaa aggcaccatt tgcagtgtat ttccaagact
    12121 acatggctaa gaaattggaa aaaggagcag gaatcactgc cgttgcagct attgttgaac
    12181 acaatacatc aggaatcatc tctcgtaaat ctgataatgt aagcagtcca aaagacttgg
    12241 ttggtaagaa atatgggaca tggaatgacc caactgaact tgctatgttg aaaaccttgg
    12301 tagaatctca aggtggagac tttgagaagg ttgaaaaagt accaaataac gactcaaact
    12361 caatcacacc gattgccaat ggcgtctttg atactgcttg gatttactac ggttgggatg
    12421 gtatccttgc taaatctcaa ggtgtagatg ctaacttcat gtacttgaaa gactatgtca
    12481 aggagtttga ctactattca ccagttatca tcgcaaacaa cgactatctg aaagataaca
    12541 aagaagaagc tcgcaaagtc atccaagcca tcaaaaaagg ctaccaatat gccatggaac
    12601 atccagaaga agctgcagat attctcatca agaatgcacc tgaactcaag gaaaaacgtg
    12661 actttgtcat cgaatctcaa aaatacttgt caaaagaata cgcaagcgac aaggaaaaat
    12721 ggggtcaatt tgacgcagct cgctggaatg ctttctacaa atgggataaa gaaaatggta
    12781 tccttaaaga agacttgaca gacaaaggct tcaccaacga atttgtgaaa taatgacaga
    12841 aattagacta gagcacgtca gttatgccta tggtcaggag aggattttag aggatatcaa
    12901 cctacaggtg acttcaggcg aagtggtttc catcctaggc ccaagtggtg ttggaaagac
    12961 caccctcttt aatctaatcg ctgggatttt agaagttcag tcagggagaa ttgtccttga
    13021 tggtgaagaa aatcccaagg ggcgcgtgag ttatatgttg caaaaggatc tgctcttgga
    13081 gcacaagacg gtgcttggaa atatcattct gcccctcttg attcaaaagg tggataaggc
    13141 agaagctatt tcccgagcgg ataaaattct tgcgaccttc cagctgacag ctgtaagaga
    13201 caagtatcct catgaactta gcggtgggat gcgccagcgt gtagccttac tccggaccta
    13261 cctttttggg cacaagctct ttctcttaga tgaggccttt agcgccttgg atgagatgac
    13321 aaagatggaa ctccacgctt ggtatcttga gattcacaag cagttgcagc taacaaccct
    13381 gatcatcacg catagtattg aggaggccct caatctcagc gaccgtatct atatcttgaa
    13441 aaatcgccct gggcagattg tttcagaaat taaactagat tggtctgaag atgaggacaa
    13501 ggaagtccaa aagattgcct acaaacgtca aattttggcg gaattaggct tagataagta
    13561 gaaaaatagg gagttggtga agattatcct ttaccagcgc cctttttctt ttaaaaatga
    13621 gaaaatttcg gtataatagt caaacaaggt caaggtttaa agagagaggt gggtttgtta
    13681 tgagatttaa aaatacatcg gatcatattg aggcctacat caaggcgatt ttagatcaat
    13741 ctggtatcgt ggagttgcaa cggagtcagt tggcagatac ctttcaggtt gttcctagtc
    13801 agattaacta cgtgatcaag acacgcttta cggaaagtag aggctacttg gttgaaagta
    13861 agcgtggtgg cggaggctac attcgtatag gacggattga gttttctagt catcatgaaa
    13921 tgctccggga gctgctttac tcgattggtg agcgagtcag tcaagaaatt tatgaggata
    13981 ttctccagct tttggttgag caggaattga tgaccaagca ggagatgaat ttgctagaat
    14041 cagtagcttt ggatcgcgtt ttaggagaag aagctccagt tgttcgagca aacatgctac
    14101 gtcagatcat acaagaggta gatagaaaag ggaagtaaga tgaactattc aaaagcattg
    14161 aatgaatgta tcgaaagtgc ctacatggtt gctggacatt ttggagctcg ttatctagag
    14221 tcgtggcact tgttgattgc catgtctaat cacagttata gtgtagcagg ggcaacttta
    14281 aatgattatc cgtatgagat ggaccgttta gaagaggtgg ctttggaact gactgaaacg
    14341 gactatagcc aggatgaaac ctttacggaa ttgccgttct cccgtcgttt gcaggttctt
    14401 tttgatgaag cagagtatgt agcgtcagtg gtccatgcta aggtactagg gacagagcac
    14461 gtcctctatg cgattttgca tgatagcaat gccttggcga ctcgtatctt ggagagggct
    14521 ggtttttctt atgaagacaa gaaagatcag gtcaagattg ctgctcttcg tcgaaattta
    14581 gaagaacggg caggctggac tcgtgaagat ctcaaggctt tacgccaacg ccatcgtaca
    14641 gtagctgaca agcaaaattc tatggccaat atgatgggca tgccgcagac tcctagtggt
    14701 ggtctcgagg attatacgca tgatttgaca gagcaagcgc gttctggcaa gttagaacca
    14761 gtcatcggtc gggacaagga aatctcacgt atgattcaaa tcttgagccg gaagactaag
    14821 aacaaccctg tcttggttgg ggatgctggt gtcgggaaaa cagctctggc gcttggtctt
    14881 gcccagcgta ttgctagtgg tgacgtgcct gcggaaatgg ctaagatgcg cgtgttagaa
    14941 cttgatttga tgaatgtcgt tgcagggaca cgcttccgtg gtgactttga agaacgcatg
    15001 aataatatca tcaaggatat tgaagaagat ggccaagtca tcctctttat cgatgaactc
    15061 cacaccatca tgggttctgg tagcgggatt gattcgactc tggatgcggc caatatcttg
    15121 aaaccagcct tggcgcgtgg aactttgaga acggttggtg ccactactca ggaagaatat
    15181 caaaaacata tcgaaaaaga tgcggcactt tctcgtcgtt tcgctaaagt gacgattgaa
    15241 gaaccaagtg tggcagatag tatgactatt ttacaaggtt tgaaggcgac ttatgagaaa
    15301 catcaccgtg tacaaatcac agatgaagcg gttgaaacag cggttaagat ggctcatcgt
    15361 tatttaacca gtcgtcactt gccagactct gctatcgatc tcttggatga ggcggcagca
    15421 acagtgcaaa ataaggcaaa gcatgtaaaa gcagacgatt cagatttgag tccagctgac
    15481 aaggccctga tggatggcaa gtggaaacag gcagcccagc taatcgcaaa agaagaggaa
    15541 gtacctgtct acaaagactt ggtgacagag tctgatattt tgaccacctt gagtcgcttg
    15601 tcaggaatcc cagttcaaaa actgactcaa acggatgcta agaagtattt aaatcttgaa
    15661 gcagaactcc ataaacgggt tatcggtcaa gatcaagctg tttcaagcat tagccgtgcc
    15721 attcgccgca accagtcagg gattcgcagt cataagcgtc cgattggttc ctttatgttc
    15781 ctagggccta caggtgtcgg gaaaactgaa ttagccaagg ctctggcaga agttcttttt
    15841 gacgacgaat cagcccttat ccgctttgat atgagtgagt atatggagaa atttgcagct
    15901 agtcgtctca acggagctcc tccaggctat gtaggatatg aagaaggtgg ggagttgaca
    15961 gagaaggttc gcaataaacc ctattccgtt ctcctctttg atgaggtaga gaaggcccac
    16021 ccagatatct ttaatgttct cttgcaggtt ctggatgacg gtgtcttgac agatagcaag
    16081 ggacgcaagg tcgatttttc aaataccatt atcattatga catcgaatct aggtgcgact
    16141 gcccttcgtg atgataagac tgttggtttt ggggctaagg atattcgttt tgaccaggaa
    16201 aatatggaaa aacgcatgtt tgaagaactg aaaaaagctt atagaccgga attcatcaac
    16261 cgtattgatg agaaggtggt cttccatagc ctatctagtg atcatatgca ggaagtggtg
    16321 aagattatgg tcaagccttt agtggcaagt ttgactgaaa aaggcattga cttgaaatta
    16381 caagcttcag ctctgaaatt gttagcaaat caaggatatg acccagagat gggagctcgc
    16441 ccacttcgca gaaccctgca aacagaagtg gaggacaagt tggcagaact tcttctcaag
    16501 ggagatttag tggcaggcag cacacttaag attggtgtca aagcaggcca gttaaaattt
    16561 gatattgcat aaaagaataa aagtatcagc atctgaccat aagtcacagt ggagtgaaat
    16621 tcaatgaaaa tcaaagagca aactaggcag ctagccgcag gttgctcaaa acactggttt
    16681 gaggttgcag atagagctga cgtggtttga agagattttc gaagagtatg aaactaaaac
    16741 ctatagcttc taaacgatcc gtggttttca tcattcaaca caaaattcat atgtttatta
    16801 ccctccgtcg tatttgtctt agagcgtgtg tagtagaaaa agagcagtct tatctgaaat
    16861 ttttattctt tcaaaagaga cctgtttctt ttttgcatgt caaatccgtt ctagctggta
    16921 tttgaaaaat caaactaata ttcaatgaaa atcaaagaac aaactaggaa gctagccgca
    16981 ggttgctcaa aacactgttt tgaggttgta gatagagctg acgtggtttg aagagatttt
    17041 cgaagagtat aagctgcaag atgaatgatt ttcttgtatt gacgttgttg ttgacaaaaa
    17101 gtagcggata aatgaaatcc attccattat catagatgat aggctggtag gaaattttca
    17161 aatagcatac aggaaataga tgtatggagt tctggtagta gaaagggaga gagatgaaca
    17221 ttttagttgc agatgacgag gaaatgatta gagaaggaat tgcagcattt ctgacagaag
    17281 agggttatca tgtcattatg gctaaggatg gacaagaggt cttggaaaaa tttcaagatc
    17341 tccctatcca tctcatggta ctggatttaa tgatgcctag gaagagtggt tttgaagtgt
    17401 taaaagaaat caatcaaaag cacgatattc ctgtcatcgt cttgagtgct ctgggagatg
    17461 aaactactca gtcacaggta tttgatctct atgctgatga tcatgtgaca aaaccttttt
    17521 ctttggtact gcttgtcaag cgtattaagg cgcttatcag acgttactac gtcatagagg
    17581 atctttggcg atatcaggat gtaacagtgg attttacctc ttacaaagca cattataaaa
    17641 atgaagaaat tgatctcaaa ccaaaggaat tactggtact aaagtgtttg attcagcata
    17701 aaaatcaagt tttaagtaga gagcagatat tggaagaaat ttcaaaagat gtagctgatt
    17761 taccttgtga tagggtcgtt gatgtctata ttcgtactct tcgcaaaaaa ttagctttag
    17821 attgtatcgt gactgtgaaa aatgttgggt ataagattag cttatgataa aaaatcctaa
    17881 attattaacc aagtcttttt taagaagttt tgcaattcta ggtggtgttg gtctagtcat
    17941 tcatatagct atttatttga cctttccttt ttattatatt caactggagg gggaaaagtt
    18001 taatgagagc gcaagagtgt ttacggagta tttaaagact aagacatctg atgaaattcc
    18061 aagcttactc cagtcttatt caaagtcctt gaccatatct gctcacctta aaagagatat
    18121 tgtagataag cggctccctc ttgtgcatga cttggatatt aaagatggaa agctatcaaa
    18181 ttatatcgtg atgttagata tgtctgttag tacagcagat ggtaaacagg taaccgtgca
    18241 atttgttcac ggggtggatg tctacaaaga agcaaagaat attttgcttt tgtatctccc
    18301 atatacattt ttggttacaa ttgctttttc ctttgttttt tcttattttt atactaaacg
    18361 cttgctcaat cctctttttt acatttcaga agtgactagt aaaatgcaag atttggatga
    18421 caatattcgt tttgatgaaa gtaggaaaga tgaagttggt gaagttggaa aacagattaa
    18481 tggtatgtat gagcacttgt tgaaggttat ttatgagttg gaaagtcgta atgagcaaat
    18541 tgtaaaattg caaaatcaaa aggtttcctt tgtccgcgga gcatcacatg agttgaaaac
    18601 ccctttagcc agtcttagaa ttatcctaga gaatatgcag cataatattg gagattacaa
    18661 agatcatcca aaatatattg caaagagtat aaataagatt gaccagatga gccacttatt
    18721 agaagaagta ctggagtctt ctaaattcca agagtggaca gagtgtcgtg agaccttgac
    18781 tgttaagcca gttttagtag atattttatc acgttatcaa gaattagctc attcaatagg
    18841 tgttacaatt gaaaatcaat tgacagatgc taccagggtc gtcatgagtc ttagggcatt
    18901 ggataaggtt ttgacaaacc tgattagtaa tgcaattaaa tattcagata aaaatgggcg
    18961 tgtaatcata tccgagcaag atggctatct ctctatcaaa aatacatgtg cgcctctaag
    19021 tgaccaagaa ctagaacatt tatttgatat attctatcat tctcaaatcg tgacagataa
    19081 ggatgaaagt tccggtttgg gtctttacat tgtgaataat attttagaaa gctatcaaat
    19141 ggattatagt tttctccctt atgaacacgg tatggaattt aagattagct tgtagacaga
    19201 ttagtttttt attaaagttc atatagggtt aacataagtg tgttattctt tgtgtagata
    19261 aaagaaagga tactaatatg gtattagcga ttattttagt aacattcttt attcgattga
    19321 tttttttaaa gcgttcgata gagaatgaga aacgaatcct tagcaatggc gggaaagaat
    19381 ttggagttga gaatacaaaa cgattaacta tggctcatat tgttttttat ctctcttgct
    19441 tggttgaggc aatggtgcac aagacaattt ttgatggcat gggcatggtt ggtttagtct
    19501 tgcttatttt ttctatgctg atgttgatgt tggtgattca cttgttggga gatatttgga
    19561 cagtgaagct tatgcttgtc aataatcaca aatatgtaga tcatatcttg tttaggacag
    19621 taaaacaccc taattacttt ttaaatattc ttcctgagtt gattggcttg accttgttga
    19681 gtcatgctta tgtgactttt gttttagttt ttccagttta tgcagttatt ttgtatcgac
    19741 gaatagctga agaggaaaag ctattacatg aagttataat cccaaatgga agcataaaga
    19801 gataaataca aaattcgatt tatatacagt tcatattgaa gtgatatagt aaggttaaag
    19861 aaaaaatata gaaggaaata aacatgtttg catcaaaaag cgaaagaaaa gtacattatt
    19921 caattcgtaa atttagtgtt ggagtagcta gtgtagttgt tgccagtctt gttatgggaa
    19981 gtgtggttca tgcgacagag aacgagggag ctacccaagt acccacttct tctaataggg
    20041 caaatgaaag tcaggcagaa caaggagaac aacctaaaaa actcgattca gaacgagata
    20101 aggcaaggaa agaggtcgag gaatatgtaa aaaaaatagt gggtgagagc tatgcaaaat
    20161 caactaaaaa gcgacataca attactgtag ctctagttaa cgagttgaac aacattaaga
    20221 acgagtattt gaataaaata gttgaatcaa cctcagaaag ccaactacag atactgatga
    20281 tggagagtcg atcaaaagta gatgaagctg tgtctaagtt tgaaaaggac tcatcttctt
    20341 cgtcaagttc agactcttcc actaaaccgg aagcttcaga tacagcgaag ccaaacaagc
    20401 cgacagaacc aggagaaaag gtagcagaag ctaagaagaa ggttgaagaa gctgagaaaa
    20461 aagccaagga tcaaaaagaa gaagatcgtc gtaactaccc aaccattact tacaaaacgc
    20521 ttgaacttga aattgctgag tccgatgtgg aagttaaaaa agcggagctt gaactagtaa
    20581 aagtgaaagc taacgaacct cgagacgagc aaaaaattaa gcaagcagaa gcggaagttg
    20641 agagtaaaca agctgaggct acaaggttaa aaaaaatcaa gacagatcgt gaagaagcag
    20701 aagaagaagc taaacgaaga gcagatgcta aagagcaagg taaaccaaag gggcgggcaa
    20761 aacgaggagt tcctggagag ctagcaacac ctgataaaaa agaaaatgat gcgaagtctt
    20821 cagattctag cgtaggtgaa gaaactcttc caagcccatc cctgaaacca gaaaaaaagg
    20881 tagcagaagc tgagaagaag gttgaagaag ctaagaaaaa agccgaggat caaaaagaag
    20941 aagatcgccg taactaccca accaatactt acaaaacgct tgaacttgaa attgctgagt
    21001 ccgatgtgga agttaaaaaa gcggagcttg aactagtaaa agaggaagct aaggaacctc
    21061 gaaacgagga aaaagttaag caagcaaaag cggaagttga gagtaaaaaa gctgaggcta
    21121 caaggttaga aaaaatcaag acagatcgta aaaaagcaga agaagaagct aaacgaaaag
    21181 cagcagaaga agataaagtt aaagaaaaac cagctgaaca accacaacca gcgccggctc
    21241 caaaagcaga aaaaccagct ccagctccaa aaccagagaa tccagctgaa caaccaaaag
    21301 cagaaaaacc agctgatcaa caagctgaag aagactatgc tcgtagatca gaagaagaat
    21361 ataatcgctt gactcaacag caaccgccaa aaactgaaaa accagcacaa ccatctactc
    21421 caaaaacagg ctggaaacaa gaaaacggta tgtggtactt ctacaatact gatggttcaa
    21481 tggcgacagg atggctccaa aacaatggct catggtacta cctcaacagc aatggcgcta
    21541 tggcgacagg atggctccaa aacaatggtt catggtacta tctaaacgct aatggttcaa
    21601 tggcaacagg atggctccaa aacaatgg
//
LOCUS       DAVKNL010000031        21505 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVKNL010000031 DAVKNL010000000
VERSION     DAVKNL010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21505
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.031"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            123..344
                     /locus_tag="VW411_001214"
     CDS             123..344
                     /locus_tag="VW411_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9793943.1"
                     /translation="MYRRLRDLREDHDLPQKQIATILSFTNSAYAKIERGEHALTADV
                     LVKLSDFYDVSTDYLLGLTDFPDKIRFRK"
     gene            791..1093
                     /locus_tag="VW411_001215"
                     /pseudo
     CDS             791..1093
                     /locus_tag="VW411_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            1249..2478
                     /locus_tag="VW411_001216"
     CDS             1249..2478
                     /locus_tag="VW411_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU9793944.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGITIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            2498..5194
                     /gene="ppc"
                     /locus_tag="VW411_001217"
     CDS             2498..5194
                     /gene="ppc"
                     /locus_tag="VW411_001217"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEU9793945.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYETIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    5277..5494
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5607..6611
                     /locus_tag="VW411_001218"
     CDS             5607..6611
                     /locus_tag="VW411_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9793946.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            6680..7546
                     /locus_tag="VW411_001219"
     CDS             6680..7546
                     /locus_tag="VW411_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9793947.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            7543..8301
                     /locus_tag="VW411_001220"
     CDS             7543..8301
                     /locus_tag="VW411_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9793948.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEDLTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            8391..10151
                     /gene="dnaG"
                     /locus_tag="VW411_001221"
     CDS             8391..10151
                     /gene="dnaG"
                     /locus_tag="VW411_001221"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU9793949.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFIEAVQILGQRVGIEVEKPLYSEQ
                     KSASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKRSSGKASEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAATLKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFLEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMEGISRPTPITMPVT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            10154..11263
                     /gene="rpoD"
                     /locus_tag="VW411_001222"
     CDS             10154..11263
                     /gene="rpoD"
                     /locus_tag="VW411_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEU9793950.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            11277..11606
                     /locus_tag="VW411_001223"
     CDS             11277..11606
                     /locus_tag="VW411_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU9793951.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            11721..12764
                     /gene="cpoA"
                     /locus_tag="VW411_001224"
     CDS             11721..12764
                     /gene="cpoA"
                     /locus_tag="VW411_001224"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEU9793952.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAVLKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            12780..14105
                     /locus_tag="VW411_001225"
     CDS             12780..14105
                     /locus_tag="VW411_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU9793953.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            14136..14312
                     /locus_tag="VW411_001226"
     CDS             14136..14312
                     /locus_tag="VW411_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU9793954.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            14371..14505
                     /locus_tag="VW411_001227"
     CDS             14371..14505
                     /locus_tag="VW411_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEU9793955.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            14569..15879
                     /gene="obgE"
                     /locus_tag="VW411_001228"
     CDS             14569..15879
                     /gene="obgE"
                     /locus_tag="VW411_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEU9793956.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEDFKKKLAENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            15889..16047
                     /locus_tag="VW411_001229"
     CDS             15889..16047
                     /locus_tag="VW411_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793957.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            17190..18449
                     /locus_tag="VW411_001230"
     CDS             17190..18449
                     /locus_tag="VW411_001230"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU9793958.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            18465..19016
                     /locus_tag="VW411_001231"
     CDS             18465..19016
                     /locus_tag="VW411_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9793959.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENKASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            19009..20286
                     /locus_tag="VW411_001232"
     CDS             19009..20286
                     /locus_tag="VW411_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9793960.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            20302..21162
                     /locus_tag="VW411_001233"
     CDS             20302..21162
                     /locus_tag="VW411_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEU9793961.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
BASE COUNT         6578 a         3693 c         4824 g         6410 t
ORIGIN      
        1 aaaacttgat acataaaaat aaaagtctat ataaaggatg gtaaaattcc tgttgtccga
       61 tttggacaat atcctaaata gttacaatat atggtctata ctttttctta ggagaaagct
      121 agatgtacag acgtttgaga gatttgaggg aggatcatga tctgccccaa aagcaaatag
      181 ctacaatact ttcgtttaca aattcagctt atgccaaaat tgaacggggt gagcatgcgt
      241 tgacggctga tgtattggtt aaactctcag atttctatga cgtcagtaca gactatttat
      301 tgggattaac tgattttcct gataaaattc gctttagaaa ataatctcct caatttcata
      361 gagtttgaaa atgagtgaga ttttttattt gccctttgac aactgaatag cctaaaatgg
      421 tactttcctc atttgtggag caaatttgaa tggctcgcca tgataagagc gattttaaaa
      481 tcatcaataa aatagagcga tactttatat gccatgatac aaatgatata caatgatact
      541 tctgaccgtt cagcctgcca acgtaaaaga gcagcaagtg aaattcttat gatgacttca
      601 tcagtcatgc cacgttgaat gtgtgagttt gttagataaa cgcaattaat cctcaaaagg
      661 ttccccgaac cttttgagtt ctacagacgc atcacgtgga gtgtgtaagc ttgttgctaa
      721 aagcgtaaaa accttggaac gaaaggaata atagactttc tgcgaaacaa aaatataata
      781 caataaaact atgaatgatg aagcaagtaa acaattgagc gatagccgtt tcaagatcct
      841 tgtaggtgtt cagcgcacga cttttgaaga gatgttagct gtgttaaaaa cagcttatca
      901 acgtaaacgc gcaaaaggtg gacgaaaaag caaattaagc ctagacgatc tccttatggt
      961 aactattcaa tacatgcgag aatagagcac ttatgaacaa attgcggctg attttggcat
     1021 tcacgaaagc aacttaatcc gtcggagtca atgggttgaa gcaactctta ttcaaaatgg
     1081 ttttacgatt tcaaattctg ccttaattct gtaaaaacag taaaattcga aggattgtaa
     1141 ggtaagagtt tttttctttc tgaaaaaatg gtataatagc aatcaaaact agaaaataaa
     1201 acggaatttg gaacagattt gtctgtatcc tagtagagtg gtgatactat gaagattagt
     1261 aagaggcact tattaaatta ttccatcttg attccctact tgcttttatc tattttgggc
     1321 ttgattgtgg tctattcgac caccagtgct attttaattg aagaaggcaa gagcgccttg
     1381 cagttggttc gaaaccaagg aatcttttgg attgttagtt tgatactgat tgccttaatt
     1441 tataaattga gactagattt tttgagaaat gagcgactaa tcattttagt tatattaata
     1501 gaaatgcttt tattgttctt ggctcgtttt attggtattt ccgtaaacgg ggcatacggt
     1561 tggatttcgg ttgcaggaat aactattcag ccagctgagt acttaaaaat cattattatt
     1621 tggtatttag ctcaccgatt ctccaaacag caagaagaaa tagctactta tgattttcaa
     1681 gttttgactc aaaatcaatg gcttccccgt gcttttaatg attggcgatt cgttctccta
     1741 gttctgattg gaagtttggg aattttccct gatttaggaa atgcgactat tttagtcttg
     1801 gtttccttga ttatgtatac agttagtgga atcgcttatc gctggttttc aaccattctg
     1861 gcgctcgtat ctgccgcttc tgtctttgtc ttgaccacta tcagcctaat cggtgttgag
     1921 accttttcaa aaattccagt attcggctat gtagccaagc gctttagtgc cttttttaat
     1981 ccttttgccg atcgtgctga tgcaggtcac cagttagcta attcttattt tgccatggtc
     2041 aatggcggtt ggtttggtct aggtcttgga aactcgattg aaaaacgagg ttatttgcca
     2101 gaagctcata cagactttgt cttttctatc gtgattgaag aatttggctt tgttggtgcc
     2161 agtcttattt tagctctctt gtttttcatg attttgcgga ttatcttggt cggtatccga
     2221 gcggagaatc ctttcaatgc catggttgca ctcggtgtcg gagggatgat gttggttcag
     2281 gtatttgtca atatcggagg gatttcgggc ttgattccat ctacaggagt gactttcccc
     2341 ttcttatccc agggtggaaa tagtcttcta gtcttatcag tggcagtagc ctttgtctta
     2401 aatattgatg ccagtgaaaa acgcgctaaa ttgtaccgag aattggaaaa tcaaccaatg
     2461 aaccttctgt tgaagtagga taaagaaagg atagtttatg tctcttcaaa aattagaaaa
     2521 ttatagtaat aaaagtgttg tgcaagaaga agtcttgatt ctaacagaat tactggaaga
     2581 tattactaaa aatatgcttg ccccagagac ctttgaaaaa ataatacagt tgaaagaatt
     2641 atcaacgcag gaagattatc aaggtctaaa ccgtctagtg actagcttat caaatgatga
     2701 aatggtctat atttcacgct atttctctat cttgcctctt ttgattaata tttcagagga
     2761 tgtggattta gcttatgaaa tcaatcatca aaataatatt gatcaggact atttaggtaa
     2821 attatctaca acgattaaat tggtagcaga aaaggaaaat gccgttgaga tcctagaaca
     2881 cttgaatgtt gtccctgttt tgacagccca tccaacacaa gtgcaacgca aaagtatgtt
     2941 ggatttaaca aatcatattc atagtctttt gcgtaaatac cgtgatgtta agttggggtt
     3001 gatcaataaa gataaatggt acaatgattt gcgtcgttac atcgaaatta tcatgcagac
     3061 agacatgatt cgtgagaaaa aattaaaagt gactaacgaa atcacgaatg ctatggaata
     3121 ttataacagc tcctttttga aagctgtacc tcatttgacg acggagtata agcgcttagc
     3181 gcaagcgcat ggtctgaatt taaaacaggc taaaccaatc accatgggta tgtggatagg
     3241 tggtgaccgt gatggaaatc catttgttac agcaaagacc ttgaagcagt ctgcactcac
     3301 tcagtgtgaa gtcatcatga actactatga taaaaagatt taccaacttt atcgtgaatt
     3361 ttctctttca actagcattg tcaacgtcag caagcaagtc agagaaatgg ctcgtcaatc
     3421 caaggataac tcgatttacc gcgaaaaaga gctttaccgt cgtgccttgt ttgatattca
     3481 atcaaaaatt caggcaacta aaacctatct gattgaggat gaagaagttg ggactcgtta
     3541 tgaaaccgcc aatgatttct acaaggattt gattgccatt cgagattctc tactagaaaa
     3601 taagggcgag tccttgattt caggtgattt tgtggaatta ttgcaggcag tagagatatt
     3661 tggtttttac ttagcatcaa ttgatatgcg acaagactct agcgtctatg aagcctgtgt
     3721 ggcagaactc ttgaaatcag caggaattca ttctcgttat agcgagttga gcgaagaaga
     3781 aaagtgtgac cttctcttga aagaattaga agaagatccc cgaattcttt ctgcgactca
     3841 cgcagaaaaa tcagaattat tagcaaaaga attagctatt tttaagacgg ctcgtgtttt
     3901 gaaagataag ttgggagatg atgtcatccg tcagaccatc atttcacatg caaccagcct
     3961 ttctgatatg ctagaattag ctattctgtt aaaagaagta ggactggtgg atacggaaag
     4021 ggcgcgtgtt cagattgttc ccctttttga aacaattgaa gacttggatc attcagagga
     4081 aacaatgaga aaatatcttt ctcttagcct tgccaaaaaa tggattgact cacgaaataa
     4141 ctaccaagaa atcatgcttg gctactctga cagtaataaa gatggcggtt acttgtcatc
     4201 atgttggacc ctctacaagg ctcaacaaca attgactgct attggagatg aatttggcgt
     4261 taaggttacc ttcttccatg gtcgtggtgg tactgtcggt cgtggtggtg ggccaaccta
     4321 tgaagccatt acatctcaac cgctcaagtc tatcaaggat cgtatccgct tgacggagca
     4381 gggtgaagta attgggaata aatacggtaa caaagacgcc gcttactata accttgaaat
     4441 gctagtatcg gcagctatta accgtatgat tactcagaag aagagcgata ccaatacccc
     4501 aaatcgttat gaaaccatta tggatcaagt agtggaccgt agttacgata tctaccgtga
     4561 tttggtcttt ggtaatgagc atttctatga ttatttcttc gagtcaagtc caatcaaggc
     4621 tatttcaagt tttaatattg gttctcgtcc agccgctcgt aagactatta ctgaaatcgg
     4681 tggtttgcgt gccatccctt gggtattctc atggtcacag agtcgtgtta tgttccctgg
     4741 atggtacggg gttggttcaa gcttcaagga atttatcaat aaaaatccag agaatattgc
     4801 tatcttacga gatatgtacc aaaattggcc tttcttccaa tcgcttcttt caaatgttga
     4861 tatggttttg tcaaaatcaa atatgaatat tgcttttgaa tatgctaaac tttgtgaaga
     4921 cgagcaagtt aaggccatct atgagactat tttaaatgaa tggcaagtta ctaagaacgt
     4981 tatcttggct attgaaggac atgacgaact cttagctgac aatccatatc taaaagctag
     5041 tctggattac cgtatgcctt actttaatat tctcaactat attcagttgg agttgattaa
     5101 acgccaacgt cgtggagaat tgtccagtga tcaagaacga ttgattcata tcaccatcaa
     5161 cggaattgcg acaggattgc gtaattcagg ttgataattt tcaagagtga atgctaaaag
     5221 tgaatatcaa aaaaattcta atagactatt gacaagtagt ttaaaaatga tataatttaa
     5281 ccattcagaa aagtaatcat acaaactttt tagagagtct gtggtagctg aaaacagata
     5341 agtggcaatg atgaaaattg ggctgaatgc tatttagaat ttgaaattat aaaaattcgg
     5401 taagcacacc ttacagtgca tctcgttatt gcgagactga gcgataggga aattccctat
     5461 aattgaggtg gtaccgcgca tcgacgtcct cacacaagtt ttttgtgtga ggattttttt
     5521 gatggaggtt agtatggaaa gaaaacgatg gcgtcgcttg tttagataag tgaaatatgt
     5581 taaaggaaat aaaaaggaga aacagaatga aaaataaacg tttaattgga attattgctg
     5641 cattagcagt cttagtagca ggaagcttga tttattcttc aatgaataaa tcagaagctc
     5701 agaataataa ggatgagaag aaaataacca agattggtgt gcttcaattt gtgagccatc
     5761 catcccttga tttgatttat aaagggatcc aagatggact tgcagaagaa ggatataaag
     5821 atgatcaagt taaaattgat tttatgaact cagaaggtga ccaaagtaag gttgcgacaa
     5881 tgagtaaaca attggttgca aatgggaatg accttgtggt tggtatcgca acaccagcag
     5941 cccaagggtt ggctagtgca acaaaagacc taccggttat catggccgct attacagacc
     6001 caattggtgc taacttggtt aaagatttga aaaaaccagg tggcaacgtt acaggggtat
     6061 ctgaccacaa tccagctcaa caacaagttg aactcatcaa ggctctgaca ccgaatgtga
     6121 aaacaatcgg agctctttac tcaagtagcg aagacaattc aaaaacacag gtcgaagaat
     6181 ttaaggctta tgctgaaaaa gcaggtctga cagtggaaac atttgcagtt ccttcaacaa
     6241 atgaaattgc ctcaactgtc actgttatga ctagcaaggt agatgctatt tgggttccaa
     6301 ttgataacac cattgcatca ggatttccaa cggttgtctc tagcaatcaa agttctaaga
     6361 aaccaattta tcccagtgcg acagctatgg tagaagtagg tggtttggca tcagttgtaa
     6421 ttgaccaaca tgaccttggt gtggcaacag gtaaaatgat tgtgcaagtc ttgaaaggtg
     6481 caaaaccagc cgatacccca gtcaatgtct tttcaactgg taagtcagtc atcaataaaa
     6541 aaatagcaca agaactaggt attactattc ctgagtctgt tctcaaagaa gcaggacaag
     6601 tcatcgaata aataaggtag ggaggaggag actcttccca ttttttaaaa agatgaatat
     6661 agaaaggatt aaataaaata tgattgtttc cattatttct caaggatttg tctgggctat
     6721 tctaggtctg ggaatcttta tgacatttag gattttaaac tttccagata tgacgacaga
     6781 aggttccttc cctcttgggg gagctgttgc tgtcactttg ataaccaaag gcgtgaaccc
     6841 atttttagcg acacttgttg ctgtaggagc aggttgtttg gctggaatgg cagcaggcct
     6901 tctttataca aaagggaaga tcccaacctt gctctcaggg attttggtga tgacttcttg
     6961 tcactcaatc atgctcttga ttatgggacg tgcgaattta ggcctgcttg gaaccaagca
     7021 aattcaggat gttttgcctt ttgattcgga tttgaatcaa ctcttgacag gtctcatctt
     7081 tgtgagtatt gttattgctc tcatgctctt tttcttggac actaaactcg gacaagccta
     7141 tattgctaca ggggataatc ctgatatggc tagaagtttc gggattcata ctggacgcat
     7201 ggagctcatg ggcttggtct tatcaaatgg tgtgattgcc cttgcaggtg ccctcattgc
     7261 tcagcaagaa ggttatgccg atgtgtctcg agggatcggg gttatcgttg tggggcttgc
     7321 aagtttgatt attggagaag ttattttcaa gagtttgagc ttggcagagc gtttggttac
     7381 tatcgttgta ggttctatcg cttatcaatt tttagtgtgg gcagttatcg cacttggctt
     7441 taatacaagt taccttcgtt tatacagtgc cttgatttta gcagtctgcc tcatgattcc
     7501 aacatttaag caaacaatct tgaaaggagc caagttaagc aaatgacagc aattgtagaa
     7561 ttaaaaaatg caaccaaaat cgttaaaaat ggctttgatg aagaaaagat tattttaaat
     7621 gatgtttcct tagaaatttt tgaacgggac tttatcacga ttttgggcgg aaatggtgct
     7681 ggaaaatcaa ctctctttaa cactatagca gggaccttat cactaactag tggaactatc
     7741 cgtattttag gtgaagatct cactaagttt tcacccgaga agcgtgccaa gtacctgtct
     7801 cgtgtcttcc aagatccaaa gatggggaca gctccccgta tgacggtcgc tgaaaatctt
     7861 ttaatcgcca agtttcgtgg tgaaaagcgt ggattgttac cacgacgctt gactagctat
     7921 aaggatgaat ttcaggcaac cattgaaaaa gtaggaaatg gtcttgagaa acacttgaat
     7981 acaccgattg agttcttatc aggtggacaa agacaggctt tgagtctctt gatggcaacc
     8041 ttgaagcgac ctgaattact cctgttagat gagcatactg ctgccctgga tccaaagact
     8101 agtgttgctt tgatggaatt gacagatgaa tttgttaaga aagatcagct aacagccctt
     8161 atgattactc atcatatgga agatgctctc aaatacggca atcgcttgat tgtcatgaaa
     8221 gaaggacgaa ttatccaaga tttaaaccaa gaagaaaaag caaaaatgaa aatctctgat
     8281 tattatcaac tctttgaata aagtagggaa aatgagtgaa atggtttact ttttttctga
     8341 aataaagtat actatataaa gtaaactatg ataacatgga ggtattgtgt atggttgaca
     8401 aacaagtcat tgaagaaatc aaaaacaatg ccaacattgt ggaagtcata ggagatgtga
     8461 tttctttaca aaaggcagga cggaactatc tagggctctg tccttttcat ggtgaaaaaa
     8521 caccatcttt caatgttgta gaggacaagc agttttatca ctgtttcggt tgtggtcgct
     8581 caggtgatgt ctttaagttc atcgaggagt accaaggggt tccctttata gaagcagtcc
     8641 aaatcttagg tcagcgtgtt ggaattgagg ttgaaaaacc gctttatagt gagcagaagt
     8701 cagcctcacc tcaccaagct ctttatgata tgcacgaaga tgcagccaaa ttttaccatg
     8761 ctattctcat gacaacgact atgggcgaag aggccagaaa ttacctttat cagcgggggt
     8821 tgacagatga agtgcttaaa catttttgga ttggtttagc acctccagaa cgaaactatc
     8881 tctatcaacg tttgtctgat cagtatcgtg aagaggattt actggattca ggcctgtttt
     8941 atctctcgga tgccaatcaa tttgtagaca cctttcacaa tcgcattatg tttcccctga
     9001 caaatgacca aggaaaggtc attgccttct caggtcgtat ctggcaaaaa acggattcac
     9061 aaacttctaa gtataaaaac agccgatcga ctgcaatttt taacaaaagt tacgaattat
     9121 atcatatgga tagggcaaaa agatcttctg gaaaagctag tgagatttac ctgatggaag
     9181 gattcatgga tgttattgca gcctatcggg ctggaatcga aaatgctgtg gcgtcgatgg
     9241 gaacagcctt gagtcgagag catgttgagc atctgaaaag gttaaccaag aaattggttc
     9301 ttgtttacga tggagataag gctgggcaag ccgcgacatt gaaagcattg gatgaaattg
     9361 gtgatatgcc tgtgcaaatc gtcagcatgc ctgataactt ggatcctgat gagtatctac
     9421 aaaaaaatgg tccagaagac ttggcctatc tattaacgaa aactcgtatt agtccgattg
     9481 agttctacat tcatcagtac aaacctgaaa acagtgaaaa tctgcaggct cagattgagt
     9541 ttcttgaaaa aatagctccc ttgattgttc aagaaaagtc catcgctgct caaaacagct
     9601 atattcatat tttagctgac agtctggcgt cctttgatta tacccagatt gagcagattg
     9661 ttaatgagag tcgtcaggtg caaaggcaga atcgcatgga aggaatttcc agaccgacgc
     9721 caatcaccat gcctgtcacc aagcagttat cggctattat gagggcagaa gcccatctac
     9781 tctatcggat gatggaatcc cctcttgttt tgaacgatta ccgtttgcga gaagactttg
     9841 catttgctac acctgaattt caggtcttat atgacttgct tggccagtat ggaaatcttc
     9901 ctccagaagt tttagcagag cagacagagg aagttgaaag agcttggtac caagttttag
     9961 ctcaggattt gcctgctgag atatcgccgc aggaacttag tgaagtagag atgactcgaa
    10021 acaaggctct cttgaatcag gacaatatga gaatcaaaaa gaaggtgcag gaagctagcc
    10081 atgtaggaga tacagataca gccctagaag aattggaacg tttaatttcc caaaagagaa
    10141 gaatggagta ataatggcaa caaaacaaaa agaagtaaca acatttgacg tacaggtagc
    10201 agaatttatc cgtaatcata agcaaaaagg gacagcaaca gatgatgaaa tcaatgcaag
    10261 tctggttatt ccttttacct tggacgctga tgggattgaa gatctcttgc aacggattca
    10321 ggatgcaggc atttctatca cagataacga aggaaatcca agtgcgcgtg ttctcagcaa
    10381 tgaagaagaa ccagaactca gcgatgagga cttgattggg tcaacttctg ctaaggtcaa
    10441 tgaccctgtc cgtatgtact tgaaagaaat aggggtcgtt cctctcttga ccaatgaaga
    10501 ggagaaagag ttggcactgg ctgttgaagc tggtgatatc gaagccaaac aacgtcttgc
    10561 ggaagccaat cttcgtttgg ttgtttccat tgccaaacgc tatgtcggtc gtggtatgca
    10621 gttccttgac ttgattcaag aaggaaatat gggcttgatg aaggcggttg acaagtttga
    10681 ctattctaaa gggttcaagt tttcaactta tgcaacttgg tggattcgtc aggctatcac
    10741 tcgtgctatt gcggaccaag ctcgtaccat ccgtatccca gttcacatgg ttgaaactat
    10801 caataaattg gttcgtgaac agcggaatct ccttcaagaa ttggggcaag atccgacacc
    10861 agaacagatt gctgaacgaa tggatatgac acctgataag gttcgtgaaa tcttgaagat
    10921 tgcccaagaa ccagtatctc ttgaaactcc tatcggtgaa gaggacgata gccaccttgg
    10981 agactttatc gaagatgaag tgattgaaaa tccagtggat tatacgactc gtatcgtctt
    11041 gcgtgagcaa ttggatgaaa tcttagatac tcttacagac cgtgaagaaa atgttctgcg
    11101 tctacgtttt ggactagatg atggaaaaat gcgcacactt gaagatgtgg ggaaagtctt
    11161 taacgtaact cgtgagcgta tccgtcagat tgaagcaaag gctttgagaa aactacgcca
    11221 accaagtcgt agcaaaccgc ttcgtgattt tattgaagac taagagtgag gaaaatatgg
    11281 cttatacaga agagcaaatt gaaaacatca aaacacggat tttaacagcc ttggaagaag
    11341 tcatcgaccc tgagttggga atcgatattg tcaatcttgg tttgatctat gagattcgtt
    11401 ttgacggtga cacagggcaa acagagattg acatgacttt gacaactatg ggttgtccct
    11461 tagcagacct tttgacagac cagatttatg atgctatgat agaggttcca gaagtaacgg
    11521 atactgaggt taaattagtc tggtatccag cctggacagt tgaaaaaatg agtcgctatg
    11581 cccgcattgc cctaggcatt aagtaaacat ataaaagcat gtgagagact gttggaaaag
    11641 cgaggaaatt tcccctcttt tcctctagtc tctcctttct tttgctgatt ttattcaaag
    11701 aaaatgatat aatagtagtt atggagaaaa agaaattacg catcaatatg ttgagttcaa
    11761 gtgagaaagt agcaggacag ggagtttcag gtgcttaccg tgaattagtt cgtcttcttc
    11821 accgtgctgc caaggaccaa ttgattgtta cagaaaatct tccaatcgag gcagatgtga
    11881 ctcactttca tacgattgat tttccctatt atttatcaac cttccaaaag aaacgctcag
    11941 ggagaaagat tggctatgtg catttcttgc cagctacact tgagggaagt ttgaaaattc
    12001 catttttctt aaagggaatt gtgaaacgct atgtattttc tttttacaac cggatggagc
    12061 acttggttgt ggtcaatcct atgtttattg aggatttggt agcagctggt attccacgtg
    12121 aaaaagtgac ctatattcct aactttgtca acaaggaaaa atggcatcct ctaccacaag
    12181 aagaggtagt cagactgcgc acagatcttg gtcttagtga caatcagttt atcgtagtag
    12241 gtgctgggca agttcagaaa cgtaaaggga ttgatgactt tatccgtctg gctgaggaat
    12301 tgcctcagat tacctttatc tgggctggtg gcttctcttt tggtggtatg acagatggtt
    12361 atgaacacta taagaaaatt atggaaaatc cccctaaaaa tttgattttt ccaggcattg
    12421 tatcgccaga gcggatgcgc gaattgtatg ctctagcgga tcttttcttg ttgcctagtt
    12481 acaatgagct ctttcctatg actattttag aagctgcgag ttgtgaggct cctattatgt
    12541 tgcgtgattt agatctctat aaggtgattt tggagggaaa ttatcgggcg acagcgggta
    12601 gagaagagat gaaagaggct attttggaat atcaagcaaa tcctgctgtc ttaaaagatc
    12661 tcaaagaaaa ggctaagaat atttccagag agtattctga agagcatctg ttacaaatct
    12721 ggttggactt ttatgagaaa caagccgctt tagggagaaa gtaaaaagtg aggtaatcta
    12781 tgcgaattgg tttatttaca gatacctatt ttcctcaggt ttctggtgtt gcgaccagta
    12841 ttcgaacctt gaaaacagaa cttgaaaagc agggacatgc tgtttttatc tttacgacga
    12901 cagataagga tgtcaatcgc tacgaagatt ggcaaattat ccgcattcca agtgttcctt
    12961 tctttgcttt taaggatcgt cgctttgcct accgaggttt tagcaaggca cttgaaattg
    13021 ctaaacagta tcagctagat attatccata ctcagacaga attttctctt ggcctgttgg
    13081 ggatttggat tgcgcgtgaa ttgaaaattc cagtcatcca tacctatcac acccagtatg
    13141 aagactatgt ccattatatt gctaagggga tgttgatccg gccgagtatg gtcaagtatc
    13201 tggttagagg tttcctgcat gatgtggatg gggttatttg ccctagtgag attgtccgtg
    13261 acttgctatc tgattataag gtcaaggttg aaaaacgggt cattcctact gggattgaat
    13321 tagccaagtt tgagcgtccg gaaatcaagc aggaaaattt gaaagaactg cgtagtaaac
    13381 tagggattca agatggtgaa aagacgttgc ttagtctttc gagaatctcc tatgaaaaaa
    13441 atattcaagc agttttagca gcctttgctg atgttctgaa agaggaagac aaggttaaac
    13501 tggtagtagc tggggatggc ccttatctga atgacctcaa agagcaagcc cagaacctag
    13561 agattcaaga ctcagtcatc tttacaggga tgattgctcc tagtgagacg gctctttact
    13621 ataaagcggc ggatttcttc atttcggcat cgacaagcga aacgcaaggt ttgacctact
    13681 tggaaagctt agccagtgga acacctgtca ttgctcacgg aaatccttat ttgaacaacc
    13741 tcatcagtga taaaatgttt ggaaccttgt actatggaga acatgatttg gctggtgcta
    13801 ttttggaagc cctgattgca acaccagaca tgaacgagca taccttatca gagaaattgt
    13861 atgagatttc agctgagaac tttgggaaac gagtgcatga gttttatctg gatgccatta
    13921 tttcaaataa cttccagaaa gatttggcta aagatgatac ggtcagtcag cgtatcttta
    13981 agacagtttt gtatcttcag caacaggtgg ttgctgtacc tgtaaaagga tctagacgca
    14041 tgttgaaggc ttcaaaaaca cagttgatca gtatgagaga ctattggaaa gaccatgaag
    14101 aatagaaaga ggaacagcta tgaaaaaaac aattaatgag aagcggtcgt gataaaaaga
    14161 ttgcgggtgt ttgtgctggg gtggcccatt atctggatat ggatccgact atcgttcaag
    14221 tcatttgggg tgttcttact tgctgttacg gagctggaat tgtagcttac attattttat
    14281 ggattatcgc accagtagca actgactatt gaaaactagc tcaattcatg ctaagataat
    14341 agaaaataga atgtaacgaa ggagagaaaa atggcatttg gagataatgg aaatcgtaaa
    14401 aaaactatgt ttgagaaaat aaccttgttt atcgtgatta tcatgctagt agcaagttta
    14461 ttgggaattt ttgcaactgc aattggtgcc ctcagtaatc tataaaatag attcaagaaa
    14521 atttagtgac tgggatttcc cagccctttt ttaaagtgag aagaaataat gagtatgttt
    14581 ttagatacag ctaagattaa ggtcaaggct ggtaatggtg gcgatggtat ggttgccttt
    14641 cgtcgtgaaa aatatgtccc taatggaggc ccttggggtg gtgatggtgg tcgtggaggc
    14701 aatgtggtct tcgttgtaga cgaaggacta cgtaccttga tggatttccg ctacaatcgt
    14761 catttcaagg ctgattctgg tgaaaaaggg atgaccaaag ggatgcatgg tcgtggtgct
    14821 gaggacctta gagttcgagt accacaaggt acgactgttc gtgatgcgga gactggcaag
    14881 gttttaacag atttgattga acatgggcaa gaatttatcg ttgcccacgg tggtcgtggt
    14941 ggacgtggaa atattcgttt cgcgacacca aaaaatcctg caccggaaat ctctgaaaat
    15001 ggagaaccag gtcaggaacg tgagttacaa ttggaactaa aaatcttggc agatgtcggt
    15061 ttagtaggat tcccatctgt agggaagtca acacttttaa gtgttattac ctcagctaag
    15121 cctaaaattg gtgcctacca ctttaccact attgtaccaa atttaggtat ggttcgcacc
    15181 caatcaggtg aatcctttgc agtagccgac ttgccaggtt tgattgaagg ggctagtcaa
    15241 ggtgttggtt tgggaactca gttcctccgt cacatcgagc gtacacgtgt tatccttcac
    15301 atcattgata tgtcagctag cgagggccgt gatccatatg aggactacct agctatcaat
    15361 aaagagctgg agtcttacaa tcttcgcctc atggagcgtc cacagattat tgtagctaat
    15421 aagatggaca tgcctgagag tcaggaaaat cttgaagact ttaagaaaaa attggctgaa
    15481 aattatgatg aatttgaaga gttaccagct atcttcccaa tttctggatt gaccaagcaa
    15541 ggtctggcaa cacttttaga tgctacagct gaattgttag acaagacacc agaatttttg
    15601 ctctacgacg agtccgatat ggaagaagaa gcttactatg gatttgacga agaagaaaaa
    15661 gcctttgaaa ttagtcgtga tgacgatgcg acatgggtac tttctggtga aaaactcatg
    15721 aaactcttta atatgaccaa ctttgatcgt gatgaatctg tcatgaaatt tgcccgtcag
    15781 cttcgtggta tgggggttga tgaagccctt cgtgcgcgtg gagctaaaga tggggatttg
    15841 gtccgcattg gtaaatttga gtttgaattt gtagactagg agactggtat gggagataaa
    15901 ccgatatctt tccgagatgc ggatggtaat tttgtttccg ccgcagacgt ttggaatgaa
    15961 aagaaattgg aagaactatt taatcgtctc aatccaaatc gtgccttgag attggcacga
    16021 actaaaaagg aaaatccatc tcagtaaaga agctaaaaaa tcccgtgcct catcagacac
    16081 gggattttgt ggtacgacag gcatgtatag caaactgaat ctggaatagc acagcatatc
    16141 ttctaaaata tagtaaaatg aaatgagaac aggacaaatc gatcaggaca gtaaaatcga
    16201 tttctaacaa tgttttataa gcagagatgt actattctag tttcaatcaa ctatattgtt
    16261 ataaattgat ttgaatttca aaattaaatt gtttgattct tatttcaatt tgttatagta
    16321 tatctgatgt caaagttctc ggcgagtcaa atagcgattc ccaagcctga ctatcgtgag
    16381 gtagcggatt aaaatggtct ggggatagac cgttttaagt ctgacgctgg aaataagaat
    16441 tgtcagaaga agggatagcg aaatcgtggc tctacgaaca ggaacgtgat aataaggcgt
    16501 atatagcgga taagagggca tcaaactcta aagtccaaaa aggtagtcgt aacctatatg
    16561 cgtaaatcac gagagtaatt gaattcgtac taagattttc tattttcact gtaacctttt
    16621 aacgccctta tatcttgtat acacgaggaa agatgtacga cttatcccgt gaggtctatc
    16681 actataaaga gaaaacgaca gatagaagtg atcctgagtc acggttatct gtctgatagg
    16741 acggtatgta taaaacgctt ctgtgaactg agagaagggg gagaagttct tgctaaaatt
    16801 tagttgaaca gccgtattcc gatacttaga taagagatct agtcttagct cctactcagt
    16861 tttaggggat aaaaaagggg caatagcgat tcgagaaaga ttatactctt cgaaaatctc
    16921 ttcaaatcac gtcaatatcg ccttgtcgta tgtgtaggat actgactacg tcagttccat
    16981 ctacaacctc aaaacagtgt tttgagcaac ctgcggctag tttcctagtt tgatctttga
    17041 ttttcattga gtattagtaa ttcagttact aactcgtcaa ctctgattta tccaataaaa
    17101 ttgaaaagga tggaaaaaag gataaattta tgatatactt tattttgaag accttattag
    17161 aaatcttgaa agagtattga aaacttagaa tgagaaaaat tgttatcaat ggtggattac
    17221 cactgcaagg tgaaatcact attagtggtg ctaaaaatag tgtcgttgcc ttaattccag
    17281 ctattatctt ggctgatgat gtggtgactt tggattgcgt tccagatatt tcggatgtag
    17341 ccagtcttgt cgaaatcatg gaattgatgg gagctactgt taagcgttat gacgatgtat
    17401 tggagattga cccaagaggt gttcaaaata ttccaatgcc ttatggtaaa attaacagtc
    17461 ttcgtgcatc ttactatttt tatgggagcc tcttaggccg ttttggtgaa gcgacagttg
    17521 gtctaccggg aggatgtgat cttggtcctc gtccgattga cttacacctt aaggcgtttg
    17581 aagctatggg tgccactgct agctacgagg gagataacat gaagttatct gctaaagata
    17641 caggacttca tggtgcaagt atttacatgg atacggttag tgtgggagca acgattaata
    17701 cgatgattgc tgcggttaaa gcaaatggtc gtactattat tgaaaatgca gcccgtgaac
    17761 ctgagattat tgatgtagct actctcttga ataatatggg tgcccatatc cgtggggcag
    17821 gaactaatat catcattatt gatggtgttg aaagattaca tgggacacgt catcaggtga
    17881 ttccagaccg cattgaagct ggaacatata tatctttagc tgctgcagtt ggtaaaggaa
    17941 ttcgtataaa taatgttctt tacgaacacc tggaagggtt tattgctaag ttggaagaaa
    18001 tgggagtgag aatgactgta tctgaagaca gcatttttgt cgaggaacag tctaatttga
    18061 aagcaatcaa tattaagaca gctccttacc caggctttgc aactgatttg caacaaccgc
    18121 ttacccctct tttactaaga gcgaatggtc gtggtacaat tgtcgatacg atttacgaaa
    18181 aacgtgtaaa tcatgttttt gaactagcaa agatggatgc ggatatttcg acaacaaatg
    18241 gtcatatttt gtacacgggt ggacgtgatt tacgtggggc cagtgttaaa gcgaccgact
    18301 taagagctgg ggctgcacta gtcattgctg ggcttatggc tgaaggtaaa actgaaatta
    18361 ccaatatcga gtttatctta cgtggttatt ctgatattat cgaaaaatta cgtaatttag
    18421 gagcggatat tagacttgtt gaggattaaa ccgtagaggt gtttatgaat atttggacca
    18481 aattagcaat gttttctttt tttgaaacgg atcgcttgta tttgcgtcct ttctttttta
    18541 gtgatagtca ggacttccgc gagatagctt caaatccaga aaatcttcaa tttattttcc
    18601 caacgcaggc aagtctggaa gaaagtcaat atgcactggc caattacttt atgaagtccc
    18661 ctttgggagt gtgggcaatt tgtgaccaga aaaatcaaca aatgattggt tctattaaat
    18721 ttgagaagtt agatgaaatc aaaaaagaag ctgagcttgg ctattttttg agaaaagatg
    18781 cttggtcgca aggatttatg acagaggttg ttagaaaaat ttgtcagctt tcttttgagg
    18841 aatttggctt aaaacaatta tttatcatta cccaccttga aaataaagct agccaaagag
    18901 ttgctcttaa gtctggattt agtttgttcc gtcagtttaa gggaagtgat cgttacacaa
    18961 gaaaaatgcg ggattatctt gaatttcggt atgtaaaagg agagttcaat gagtaagcat
    19021 caggaaattc taagctattt ggaggaatta ccagtaggta aaagggtcag tgttcgtagc
    19081 atttcgaatc atctaggagt tagtgatgga acagcctatc gggctattaa agaagctgaa
    19141 aaccgtggaa ttgtggagac ccgtcctaga agtggaacaa ttcgtgttaa atcccagaaa
    19201 gttgctatag agagattaac gtttgctgaa attgcagaag tgacttcttc tgaggttctg
    19261 gctgggcaag aaggtttaga gagagaattt agtaagtttt caattggtgc catgactgaa
    19321 caaaatatct tgtcttacct tcatgatggg gggctcttga ttgtcggaga ccgaacccgt
    19381 attcagttgc tagccttgga aaatgaaaat gcagttctgg ttacaggggg atttcaggtt
    19441 catgatgatg tgcttaaact ggccaatcaa aaagggattc ctgttctaag aagtaagcat
    19501 gataccttta ccgtcgcgac catgatcaat aaagccttgt caaatgtcca aatcaagact
    19561 gatattctga cagttgagaa actttatcgc cctagtcatg agtatggttt tctgagagag
    19621 acagatacag ttaaagatta tttggacttg gttcgtaaga atcgtagcag ccgtttccct
    19681 gttatcaatc aacatcaggt cgttgttggt gttgtaacca tgagagacgc tggtgataaa
    19741 tcaccaagca cgacaattga taaggttatg tctcgtagtc tatttttggt tggattatcg
    19801 acaaatattg ccaatgtgag tcaacggatg atcgcagaag actttgaaat ggtaccagtt
    19861 gttcgaagca atcaaacttt gcttggcgtt gtgacgcgac gagatgtcat ggagaagatg
    19921 agccgttccc aagtttcggc tctaccaact ttttctgagc agattggaca aaagctctct
    19981 tatcaccatg atgaagtagt cattacagtg gaacccttta tgctagaaaa aaatggagtt
    20041 ttggctaatg gtgtattggc agaaattctg acccacatga cccaagattt agttgttaat
    20101 agtggtcgca atctcattat cgagcagatg ctgatctact ttttgcaggc tgttcagata
    20161 gatgatatat tgcgcattca ggcacggatt attcatcata cgagacggtc agctataatt
    20221 gattacgata tttatcatgg tcaccagatt gtttcaaaag caaatgtgac tgttaaaatt
    20281 aattagaaac taggagaaaa gatgataaca ttaaaatcag ctcgtgaaat cgaagctatg
    20341 gacaaggctg gtgattttct agcaagtatt catataggct tacgtgattt gattaagcca
    20401 ggcgtagata tgtgggaagt tgaagaatat gtccgccgtc gttgtaaaga agaaaatttc
    20461 cttccacttc agattggggt tgacggtgcc atgatggact atccttatgc tacctgttgc
    20521 tctcttaacg atgaagtggc tcacgctttc cctcgtcatt atatcttgaa agatggtgat
    20581 ttgctcaaag ttgatatggt tttgggaggt cccattgcta aatctgacct aaatgtctca
    20641 aaattaaact tcaacaatgt tgaacaaatg aaaaaataca ctcagagcta ttctggtggt
    20701 ttagcagact catgttgggc ttatgctgtt ggtacaccgt ccgaagaagt caaaaacttg
    20761 atggatgtaa ccaaagaagc tatgtacaag ggtattgagc aagctgttgt tggaaatcgt
    20821 atcggtgata tcggtgcggc tattcaagaa tacgctgaaa gtcgtggtta cggtgtagtg
    20881 cgtgatttgg ttggtcatgg tgttggccca actatgcacg aagaaccaat ggttcctaac
    20941 tatggtattg caggtcgtgg actccgtctt cgtgaaggaa tggtcttaac cattgaacca
    21001 atgatcaata caggcgattg ggaaattgat acagatatga aaactggttg ggcgcataag
    21061 accattgacg gtggattgtc atgtcagtat gaacaccaat ttgtcattac gaaagatgga
    21121 cctgttatct tgactagcca aggtgaagaa ggaacttatt aataaaaagt gaaaagacta
    21181 ctggaagttt attttgataa aaaatccagt agatcttttc ataataaaac gcattgtatc
    21241 aagtgttagg ggctgatatc atgcgttttt ctgcttttaa gattttttcc aactctgttt
    21301 gtaagcgcat cataacaaag ggtctaggat tcagggctct cctcctatat actattagta
    21361 aagtaaaact aagggaggat attttagtgt cgcagtctat tgttcctgta gagattccac
    21421 aatattgtcg ttttgattct aaaaagagaa atggaattct gtttaatgtt cgtattgcca
    21481 atcttaaatt tactttttta tatta
//
LOCUS       DAVKNL010000032        21009 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVKNL010000032 DAVKNL010000000
VERSION     DAVKNL010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21009
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.032"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            1..596
                     /locus_tag="VW411_001234"
                     /pseudo
     CDS             1..596
                     /locus_tag="VW411_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(589..762)
                     /locus_tag="VW411_001235"
     CDS             complement(589..762)
                     /locus_tag="VW411_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HEU9793962.1"
                     /translation="MNIKKRVLSAGLTFASALLLAACGQSGSDTKTYSSTFSGNPTTF
                     NYLLDYYADNIVN"
     gene            complement(921..2528)
                     /locus_tag="VW411_001236"
     CDS             complement(921..2528)
                     /locus_tag="VW411_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEU9793963.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGVGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENTDSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKFYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLVRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTAAKEVLEKQVLAPWDAFCVELL"
     gene            2681..4165
                     /locus_tag="VW411_001237"
     CDS             2681..4165
                     /locus_tag="VW411_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEU9793964.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            4260..4742
                     /locus_tag="VW411_001238"
     CDS             4260..4742
                     /locus_tag="VW411_001238"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEU9793965.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(5038..7143)
                     /locus_tag="VW411_001239"
     CDS             complement(5038..7143)
                     /locus_tag="VW411_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9793966.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQGGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELERKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMEASDIERLKDMAHRLQDKVIGQDKAVEVVARAIRRNRAGFDEGNRPIGNFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKV"
     gene            complement(7752..8732)
                     /locus_tag="VW411_001240"
     CDS             complement(7752..8732)
                     /locus_tag="VW411_001240"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEU9793967.1"
                     /translation="MFISISAGIVTFLLTLVEIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDILSVFGYPVHLGFFYIFFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            complement(8734..10986)
                     /gene="pbpX"
                     /locus_tag="VW411_001241"
     CDS             complement(8734..10986)
                     /gene="pbpX"
                     /locus_tag="VW411_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HEU9793968.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRTMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGLT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            complement(10990..11307)
                     /gene="ftsL"
                     /locus_tag="VW411_001242"
     CDS             complement(10990..11307)
                     /gene="ftsL"
                     /locus_tag="VW411_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEU9793969.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            complement(11319..12269)
                     /gene="rsmH"
                     /locus_tag="VW411_001243"
     CDS             complement(11319..12269)
                     /gene="rsmH"
                     /locus_tag="VW411_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEU9793970.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQACLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNNYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVVRKIHK"
     gene            12435..12629
                     /locus_tag="VW411_001244"
     CDS             12435..12629
                     /locus_tag="VW411_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9793971.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            12645..13187
                     /locus_tag="VW411_001245"
     CDS             12645..13187
                     /locus_tag="VW411_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEU9793972.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADSIGNQVFIILFYLM
                     IFGNLIPLLLAYKYPQEVALIYPPLILVIALIASGYVTYQMKKTGITVIEPDMLNEKE
                     SKQLHYPGLKAGLFFGLWMFFITPLLRILIDEGQDYFHSLLTIRNGVSSILGSIFFGA
                     SIQFLISRRIAKTKKNQDED"
     gene            13198..13440
                     /locus_tag="VW411_001246"
     CDS             13198..13440
                     /locus_tag="VW411_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793973.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            13451..13786
                     /locus_tag="VW411_001247"
     CDS             13451..13786
                     /locus_tag="VW411_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9793974.1"
                     /translation="MKSLARLLIIHVFISIFLFFALTSGAISHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(13896..14897)
                     /locus_tag="VW411_001248"
     CDS             complement(13896..14897)
                     /locus_tag="VW411_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9793975.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(14957..16858)
                     /locus_tag="VW411_001249"
     CDS             complement(14957..16858)
                     /locus_tag="VW411_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEU9793976.1"
                     /translation="MKEERRQFFERVDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWSYMLNRQEYLLQFMIGYLVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSYYRAKYTLSNWGILQTIPMLAIYHFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEILSLSAVVLNQEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     KRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHRRKILMLVEDVWLLVDDIRCQGQHEVLTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDIGVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            complement(16880..17173)
                     /locus_tag="VW411_001250"
     CDS             complement(16880..17173)
                     /locus_tag="VW411_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU9793977.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTFGDEVLLLSGIHGKIISIQDDLVSLQIAKGVVIHVEKESVMGKTKELLFK"
     gene            complement(17173..17991)
                     /locus_tag="VW411_001251"
     CDS             complement(17173..17991)
                     /locus_tag="VW411_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9793978.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            complement(17978..18757)
                     /locus_tag="VW411_001252"
     CDS             complement(17978..18757)
                     /locus_tag="VW411_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9793979.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            complement(18772..19263)
                     /locus_tag="VW411_001253"
     CDS             complement(18772..19263)
                     /locus_tag="VW411_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU9793980.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVTIKE
                     INIGNIHNAPGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            complement(19274..20464)
                     /locus_tag="VW411_001254"
     CDS             complement(19274..20464)
                     /locus_tag="VW411_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="unsaturated chondroitin disaccharide hydrolase"
                     /protein_id="HEU9793981.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            complement(20476..20910)
                     /locus_tag="VW411_001255"
     CDS             complement(20476..20910)
                     /locus_tag="VW411_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9793982.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
BASE COUNT         6143 a         4759 c         3767 g         6340 t
ORIGIN      
        1 tggctgtcat ccaactacca tccactatgc gctcaaagct atgggctaca ctcgaaaaaa
       61 agaaccacac ctactatgaa caaaacccag aaaaagtagc cttatttctt aagaatttta
      121 atagtttaaa gcacctagca cctgtttaga ttgatgaaac aggattcgat acttattttt
      181 atcgagaata tggtcgctca ttaaaaggtc agttaagaag aggcaaagta tctggaagaa
      241 gatatcagag gatttctttg gttgcaggtc taacaaatgg tgagttaatc gctccaataa
      301 cttacgaaga gacgatgacg agcgactttt ttgaagcttg gtttcagaaa tttctcttac
      361 caacattaaa cacaccatcg gttattatta tggataatgc aagattccat agaatgggta
      421 agttagaact cttgtgtgaa gagtttgggt ataaactttt acctcttcct ccctactcac
      481 ctgagtacaa tcctattgag aaaacatggg ctcatatcaa aaagcacctc aaaaaggtat
      541 taccaagttg caataccttt tatgaggctc ttttgtcttg ttcttgtttc aattgactat
      601 attatcagcg taatagtcta atagatagtt aaatgtagtt ggatttccac taaaggttga
      661 tgagtaagtt tttgtatctg aacctgattg gccgcaagca gctaaaagca aagcagatgc
      721 aaaagtcagg cctgcactaa ggacacgctt ctttatgttc atcttctttc tccttaatag
      781 tgggaatttg taaagttaat tgaatttcaa gaatgaaagt ttataaactt tggttataaa
      841 aaacaaagga tttctgtctt ttatacagtc ctcccttgtt tttatacgat ttcaatttta
      901 aatttttctg caaaaaatat ttatagtaat tccacacaga aagcatccca tggagccaag
      961 acctgttttt caagtacttc tttagccgca gtgttttcaa tcaagacaga tttgactttt
     1021 ccttctactg tcaagtcttg ctcttcattg gacaagttag ccacaactag gaagcgacgg
     1081 tcgccatcct tacgtatata agcaaagacc ttatcagccg tatcaagcaa ttcaaagtca
     1141 gctcgaacta gccagctatt ctccttgcga atttggacca gtttctgata ggtatagaaa
     1201 atagaatctg gatttgccag cgcttcttgg acattgatca tctcgtaatt tggattaacc
     1261 gccaaccaag gttgacctgt tgagaaacca gcgtttttgc tctcgtccca ttgcataggg
     1321 gtacgggcat tgtcacgtcc aataacacgg atactgtcca tgatttcttc aatcggaaca
     1381 cctttttcaa gagcctcacg cgcatagttg agagattcaa tatcttctac ttgatccagt
     1441 gtttcaaacg gatagttggt catcccaatc tcctcacctt ggtagatata aggagttcct
     1501 ctcatgagat gaagtaagat tgcaaaggct ttggcagatt tttcgcggta ttcttggtca
     1561 tttccccaga ttgagacaat acgagggagg tcatggttgt tccagaagag ggaattccag
     1621 ccgtcctcaa ctcctaactc tgtctgccat ttgttgaaga tttcttttaa cttagcgata
     1681 ttcagctctt tttgatagtg ccatttaggc tgaccttcct gatactgaag accgatatgt
     1741 tcaaactgga agaccataga caattcttgc ccctttggat cagagtagaa cttggcaatc
     1801 tctggagttg ctccccaagt ctcccctact gtcaagagat ccttatctcc aaaggtcgcc
     1861 tgattcattt ccttgagata gggatggagc ataggaccat tattgactac cttctcgtca
     1921 ggaattttgc caatcatgtc aataacatcc atacggaaac caccaatacc tttatcaatc
     1981 cagaagttca tcatctcata aattttctgg cgaagttttt cattttccca gttgagatcc
     2041 ggctgtttct tgctgaaaaa gtggagatag tattgacctg acttttcatc gtattcccaa
     2101 gcagacccac taaagataga atctaggtca ttgggttcat cgcgccagat atagtagtct
     2161 cgctcagggc tgtcagtatt ttcacaggct tcgacaaacc aagcatgttc atctgaggta
     2221 tgattgacca ccaagtccat gatgatacga atgtcacgct tcttagcttc tgcaatcagc
     2281 tgatccatgt cctccatggt tccaaaaata gccgcaatcg cttgataatc agcaatatca
     2341 tagccattat catccatagg gctgtcataa acgggagaaa gccaaattgc tgtgattcct
     2401 agcttagcta gatagtccaa cttactggta atacctggca aatcaccaac tccatctcca
     2461 ttactatcca taaaactctt tggatagact tgatagacta cggcattgtg ccaccatttt
     2521 tcttgcatct gtttacctca ttattttcat aatctatagt ctatgatagc actttttaaa
     2581 attttgtgca agcgtttgct taatcttttt aatttctttt ttagctccgt agtttcctaa
     2641 cttccaattt tttattataa tagagtccag aggtaaaaaa atgaaaaaac aagcttttag
     2701 ttctgaacaa tatttgaatt tacaacgcga ccatattttg gagcgcatta accaatttga
     2761 cggcaagctc tacttagagt ttggcggtaa aatgttagaa gatttccacg ctgctcgtgt
     2821 ccttcctggt tatgaacctg acaacaaaat caagctcttg caagaattga aagagcaggt
     2881 tgaggttgtg attgccatta atgctagcaa cattgaacat tccaaagcac gtggcgactt
     2941 aggcatttct tatgaccaag aagttcttcg tttgattgat aaattcaatg aattagggat
     3001 ttttgttggt tccgttgtca ttacacaata cgctggccaa cccgctgcag atgccttccg
     3061 caatcaactt gagaaaaacg gaattgattc ttatcttcat tatccaatca aaggatatcc
     3121 gacggatatg gatcacatca tttccccaga aggcatgggc aaaaacgact acatcaaaac
     3181 cagtcgcaac ttgattgtcg taaccgctcc tggacctggt tctggaaaat tggcaacgtg
     3241 tatgtccaat atgtaccacg accaaatcaa tggcatcaaa tctggctacg ctaaatttga
     3301 aaccttccct gtttggaatc ttccccttca tcatccagtt aacttggctt atgaagctgc
     3361 cacagctgac cttgatgatg tcaacatgat tgaccccttc catcttcaaa cctatggaga
     3421 aaccactgtc aactacaacc gtgatatcga aatcttccca gtgctcaaac gcatgttgga
     3481 acgtattctc ggaaaatcac catacgcttc accgacagat atgggtgtca acatggttgg
     3541 tttcgctatt acagatgacg aggccgctgt cgaagcttct aaacaagaaa tcatccgccg
     3601 ttactatcaa acagttcttg acttcaaagc tgaaaaagtt ggcgaagctg ccgtcaagaa
     3661 aattgagttg ctcatgaacg acctcggtat cacacctgca gaccgtaagg ttgctgtcgt
     3721 tgcgcgccaa aaagcagaag aaactggtgg accagcccta gcctttgaat tgccaaatgg
     3781 ggaaatcgtc actggtaaga actcagaact ctttggtcct acagccgctg ccttgatcaa
     3841 cgccatcaaa aaatcagctg acatcgctaa agaagtaaaa ctaatcgagc ctgaagttgt
     3901 taagccaatc caaggtctta aaatcgatca tctcggtagc cgcaatccac gccttcattc
     3961 aaatgaaatc ctgattgcac ttgctatcac agctacagaa aatcctgatg ctgcccgcgc
     4021 tatggaagaa ctcggcaacc tcaaaggaag cgaagcccac tcaaccatca tcttgactga
     4081 tgaagacaag aatgtccttc gtaaactggg tatcaacgta acctttgacc catactacca
     4141 atacgaccgc ttatatcgta agtaaagatt tcaacctctc cagcctcgga gaggttttct
     4201 ctctgtctca ggagcaacct ttatgatata atgagagaag aaaactgaaa ggatttacca
     4261 tgtcaaaaga agttattgtc gaaagttttg aacttgacca caccattgtc aaagcaccct
     4321 atgttcgctt gattggggaa gaaacaggac caaaaggaga catcatctcc aattatgata
     4381 ttcgcttggt gcaaccaaac gaagactcta tccctactgc cggccttcac actatcgagc
     4441 acctcttagc caaactcatc cgtacccgca ttgacggcat gattgactgt tcaccatttg
     4501 gttgccgcac aggcttccac atgattatgt ggggacgtca caccagtgct aaaatcgcag
     4561 ctgttatcaa ggattcgctc aaggaaatcg ctgaaactac tacttgggaa gatgtcccag
     4621 ggacaaccat cgaatcttgc ggaaactaca aggaccacag cctcttctct gctaaagaat
     4681 gggcaaaact catcttggaa caagggattt cagatgatgc ctttgaacgt catgtgattt
     4741 aaaatcgagt aggggataat ctctagcccc tctcacacca ccgtacgtac cattcggcat
     4801 acggcggttc aactaacttt taacgcatgt cgttcaaggt aataatccaa acacgaaacc
     4861 agtccacgtt tttccaggac tggttttgat atagcacgtt taagtaccga cttctgagct
     4921 actatagtat attgaaacta gaataataca cttctactat acgacatgcc tgtcgtacct
     4981 aaaaaggaac cagttttttt cagctggctc cttctttttt atcctcaaaa ttctgtctta
     5041 gactttctca cgaataacca aaacgccatc ttccatatct gcttccagat gtttagcatc
     5101 taaatgatcc aagtggaagt ctgtcacctt atcacgaatt tcttgttcaa ccacgcgacg
     5161 gagaggacga acccccatga cttcgtcgta accttcttct gtgatataat ctttagccgc
     5221 ttgactgact accaagtcaa tgtctttctt agccaaggtt tggttaactt cagccaacat
     5281 caaatctaca atcttagaaa ggtcttcctt agtcaagtgt gagaactcga tgactgcatt
     5341 aaagcggttg aggaattctg gacggaagaa gggtttcaaa cggtccatca attctggttt
     5401 atccgcatct tctgtcaagt tggcttcata gccaaatcca gcatttgagg tcgcaataat
     5461 gacagtgttc ttgaagttta ctgtatttcc ttgaccatct gtcaaacgac catcatctag
     5521 aacttggagg agaagggtaa taacttgagg gtcagccttt tcaatttcat ccaagagaat
     5581 gatagagtat ggattgcgac gaacacgttc tgttaaggta ttgctattgt catcataacc
     5641 cacatagcct gctgttgtac caattagctt agaaacagct gtgcggtcac tgtattcaga
     5701 catatctaaa cggataatcg catcctgggt tccaaacata tcgagtgcca attgcttagc
     5761 aagctccgtc ttaccaaccc cagtagaccc tacaaagagg aagttgccga ttgggcgatt
     5821 tccttcatca aaaccagcac ggttacgacg gatagcacga gctacaactt ctacggcctt
     5881 atcttgacca atcaccttgt cttgcaagcg atgagccata tctttcaaac gttcgatatc
     5941 tgaagcttcc atttgcgata ctgggatacc tgtcattcgt tccacagatt cagccacatc
     6001 gttgacactt gcagtcactt tcatatcttc tgtgtggttt tcgattttcc tttccaattc
     6061 tgcaatgcgt gttttatagt ttagagctgc ttcaaaatct tctgcttcaa ctgctttttc
     6121 ttgcttgtct ttttccgttt cgatttctcg ttcaacagca tgcacatctg ttactggatg
     6181 ttgagccgcc aagtgagcag ccgttacatc gacaaggtca atagccttat ctggcaagct
     6241 acgttgagga atgtattgaa cagaataatc caccgctgct ttcaagactt cgtctggcaa
     6301 gatgacattg tggtgttgtt gatagaggtc acgaattcct tgaagaattt taaaagtatt
     6361 ctctgccgaa ggagcattga ccttcacttc gttgaaacga cgagcaagag cagcattctt
     6421 caagatggtg ttacggtatt cgtcttgagt tgttgcccca atcactgtca attctccacg
     6481 agagagagct ggcttgagaa tatccgcaag tcctttagaa ccactgtctc caccagtgct
     6541 accagcacca agaatttggt gaatttcatc aaagaagagg ataatattcc ctgcttcttt
     6601 cacttcattg actaagtttt ggacattttc ttcaaagcta ccacggtatt gagtaccagc
     6661 ctcaagacct gagatatcaa tagaaataat ttccttgttc ttgatagcag caggaacatc
     6721 tccgttcaca atggcttgcg ctagaccttc gacaactgct gtcttaccaa cacctgcatc
     6781 tccgaccaaa acaggattgt tcttggtgcg gcgtgagagg atttcagatg tttcttgaat
     6841 ttccttgttt cgtccgataa caggatccaa cttgccctca cgcgcttctg ctgttaagtt
     6901 tcgacctagt tttgcaagga caccgccttg tttcatacct gatgcctgtt gtggcatttg
     6961 cacatcagtt tctgcatttc ctggtaattg accagtcgca cgatagtgag caaattcctc
     7021 aggtgtgact tcgcgtccat taattaagta acggcgattt tcagaactgt atcctcgcat
     7081 accacccatc aattggttaa ataaatcatc catgttatta aaattattaa agttgttatt
     7141 catattcttt acctcttttt gtttacttag ttatgattac tgatattgac tatctttgac
     7201 cattgtttta aaaaatttag actagctaga tcgctaccct acgtactatt tctggttttt
     7261 ctttttcaag caggagtaga ctatctttac ttctgaagat ttctagatga aacatagacc
     7321 cacctctttc tattttactt taaataagtg ttctagtaag gctttcattt accttacgtt
     7381 cataatatac tacattggtc agaaaaggtc aagagttttg actttaactg accaatattt
     7441 ttttacactc ttcgaaaacc tcttcaaacc acatcagctt ctccttatct acaacctcaa
     7501 aaccgtgttt tgagctgact tcgtcagttc tatcaaccat ctcaaaactg tatattaaaa
     7561 cgaaataaga acaggacaaa tcaatcagga cagtcaaatc gatttctaac aatattttag
     7621 aagtagatgt gtactattct agtttcaatc tactatattt tgagcagcct aaagttagct
     7681 ttctagtttg ttctttgatt ttcattgtgt atttaaacac aaaaacaccc tgaaacatca
     7741 gggtgccatt cttacatcaa atataaaatt gctagggtca ggagacttgc tagaagtccc
     7801 actccccaaa agaagaagtc aaccttccac tcgctccaag gatttccttt accagacaat
     7861 cccccaagct caaaatggtg atgtacaggc gtcatacgga aaatacgttt accacctgtc
     7921 agtttgaaat aactgacttg catcataaca gaagttgttt caaaaacata cacaattccg
     7981 ataatcaaga gagtccattc ttggtggaga gccatagaga tagctgccag catcccacct
     8041 agggccaaac ttcccacatc acccataaag accttggcag gcttatggtt aaagatgaag
     8101 aaaccgagca aaccaccaat catggcaaga atcactagaa gaatatccat ctgaccttgc
     8161 acataggcaa taactccata ggcagacaaa ctaatcacaa cggaaatact agctaaaccg
     8221 tcaacaccgt ctgtcaagtt tactgcgttt gaaaaaccga ctagccagaa aagagcgaag
     8281 aaaatataga aaaatcccaa atgaactgga taaccaaaga cagacaggat atcgccaccg
     8341 cgctcataga aaagatagaa gataactcca cctagaagct gaagagctaa tttttgctta
     8401 ggattaagcc cctcattgat tttacgaaag accttgagaa agtcatctaa aaatccgacc
     8461 aagccataca agaccaagat gaacaaaatc attcccacat tattgctgaa ttggctacta
     8521 aatagggcga aaaagaaagc aaccaaaaca gaagtaatca agaaaaccaa acctcccatt
     8581 gtaggagtcc cagcttttgc ctgatgctgt ttgacatcct catgcatctg ctggcctgta
     8641 atttgcgcct ttctataaaa ttggataaag gccggaattt ctactaaagt tagtaaaaat
     8701 gtcacaattc cagcactgat ggaaataaac atattagtct cctaaagtta atgtaatttt
     8761 tttaatgtcc ttgatagctg tgttagcacg aacatcttgc ttctgcacag tagagcccga
     8821 accttgaaat tcaagttcta tattgagcca cttagcaagg gtctcagcag tctcctttgt
     8881 ccaaccatac atatctggaa cctcctctgc tttatcagat aagataagga cttgctggtt
     8941 cggggcaaga ttcttccctt cttcagcaga actgttttta atcttcgttc ctgttcccac
     9001 aacgatgggt tgtacaagat tgcgacgcaa ttcttctgct aaatcaccag gtgaaatatc
     9061 cttgacacta ggcataggat aaggactttg ttgacttact tgctctaaag ccttagctgt
     9121 tgtttgaaga ttgagagagt ctttcatagc tgaagcccgc tccaagatag gattggcaaa
     9181 ttctcccaac tgaatacctg aataatgttc aggttgttgg accgtcacat acaagataaa
     9241 atcaggattt tcagccggac tcatcgatac agccgagaaa atatagtcgg ttaacccgac
     9301 tagataacca ccatttttct cgtcagcaat ctgagccgta ccagacttga gggctacatt
     9361 ttgcccagga acagttacag ttggcttgcc tgtgctgtgg ttatacatgg ttccataaac
     9421 cggatccgtc cctaccaaaa ccatgttagt ccgagttaga ctagctgcat ctttagaaac
     9481 aggatttccc acaatttctt tttgagattt ccgagcagtt tgatcatttg gatcataaat
     9541 ggcactaata aatttaggct ccagcatgac accgtcatta gcaatagctg taaaggcacg
     9601 aatcatttgc gtctgggtca ctgaaatccc ttgtccaaat gagctttgcg caatgttgac
     9661 aatattatcc gcaggaagct gaccagcata ctcatccgtc aaaccgaaac gggtcggaac
     9721 tccaaattta aaacgattaa gataatcaag ccaggtagca tctcccatct tttgctcaag
     9781 gagggtcatc ccaacgttac ttgagtgtgc aaaaccttga gaaaaagtca tcgttctgcc
     9841 accagtcaat ccttcattaa cgtcccaatc tcgaatcgtg gcatctgcaa tttttaactc
     9901 actactatta aagacttctc ctcctggaaa ggtattatta tcaatagcag cagccaacat
     9961 catcactttc atagtggaac ctggctcata gttactttgg taaaggatat cacgccaaac
    10021 aaagtcctct gtaatgcctt cttttgtatc tgcatcaaag gtcggtcgtt gcgttgttgc
    10081 cagaatttcc cctgttttag cactgaccaa agtcgctgtc atgtactttc cttttacctt
    10141 ctcttgaaaa gcatccatct gggtttccat aaaggactgg agggggctgg aaatggttgt
    10201 ataaacatcc ttaccgtcca tcgttcgttg ggaaacttgt tctgttccgg gtacaatatt
    10261 acccagacga tccttttcat aggtaataat gccgtctgtc cctgcaagaa tactgttcaa
    10321 ggaactctcc attccagagg ttcccagcaa gctcttgctt ccatcttcat tttcatggag
    10381 ctgagctaga ccgataaaac tagaagcaaa ttgtccgttt gggtaactac gattgggact
    10441 ggttgtaaaa tcaatcccct tgacctctgc agcttccaat tcttttttga tagacatcat
    10501 attggcatag gtaatcccat ttccctttgc tccaaaggaa acttgcttga gattaggttg
    10561 cgagagttgc tctcttacat aggattcttc catgtccaga tacttatgaa agacctctgc
    10621 aaccttgtta aattgtgttt tttctacgta aagaatctta cccgttgctg acttatagtt
    10681 ctcatcaatg accgcataga cattatagga ggttgcatcc tcagcaatcg ggactccatt
    10741 tcggtcataa atagtcccac gtttggcagg aactgtacgg gtggtttgat gaaccttctt
    10801 agcttccttc gctaaatctg ttccaaagcg agtgcctgtc ccaataatga ccgcaaaatt
    10861 gactaaaaaa atggcaaaaa caaagacaga taataaactc agactttttc caactctgcg
    10921 tctgttttca gccggcgatt tccgattttt ggtcgcataa cggattactc tttttgtcca
    10981 cttcatatct tactccgcta ttctaatatt ttcattgttt aattgcaaat cgtgtgaatt
    11041 ggcaatttct ttcaaacgtt ctgcacgtaa tagttcattg acctcttgct tggcatcgtc
    11101 caattcggtc ttcttttcct ctatctgcgc attgattttt gtcaaatcat tctgcacttg
    11161 caagagcttg gtctgcataa aaataatact aatggctaca ataagagtgg ttacagcaat
    11221 ggaaaagtaa aaagcttttt ccacacgcga aaaccgttta agttgcatct gtagtatttg
    11281 acctgttttt tccatttttt ctgccatctt tttccctctt acttgtgaat ttttctgacc
    11341 acgcgcaact tggctgagtg cgagcggtta ttggcttcta actcttccgc acttggcaag
    11401 attggcttac gggacaccaa ttccatcttg ggcttgagat catctgggat gaaaggcaag
    11461 ccttttggaa cttcaactgt tgaagcttcc ttgaacaatt gcttggtcaa gcggtcttct
    11521 aaggaatgaa aggtaatcac tgaaattcta ccatccagag ccaacatatc catagcctgc
    11581 tggatggact catctgccgc tcccagttca tcattgactt caattcgaat agcctggaaa
    11641 atctgcttag caggatgccc cttcttcttg agttccttgg caggtttgac caacttgata
    11701 atctctgcta actcagtcgt tgtctcaatc ggcttcactt cacgcgcttg ctcaatctta
    11761 cgcgcaatct gtttagagaa tttgtcctct ccatacttga agaaaatacg aaccaagtca
    11821 tgatagtcat aattgttcac cacttcatag gctgtcaggc tagcatcctg attcatccgc
    11881 atgtccagtg gcgcatcctt tttataagaa aaaccacgct cacgctggtc taattgagga
    11941 ctagacactc ccaagtcata acaaattcca tcaatttcct gaacaccagc ttcgcgcaaa
    12001 catgcctgta aatgacggaa gttgtccttg ataaaggtca ccattccctt ctcaatgtaa
    12061 ggtgccaagc gtttttgcgc attgtcaatg gcattctgat cctggtcaaa ggcatagaga
    12121 tggccttttt cacttaattt acttaataaa tactcgctat gtcctgctcc gcccaaagtc
    12181 gcatcaacgt agataccatc aggctttacg tcaagcatat caatcgtttc gtggagtaag
    12241 accgttacat gatgaaattc ttttgtcata tcttatctat tttaccacaa atccgaccag
    12301 cttgcacttg tcagacaaat aggacaagga gactactatt tcttcaaaaa atatgtcaaa
    12361 aatacttgac actttatgtt taaaagacta taatatagtc aaatatatac gacataaatc
    12421 agaaaggaga acagatgaat cgtgtgaaag aatttcgcaa ggaattgggt atttcccagc
    12481 tcgaactcgc caaggatatc ggtgtctcga gacagactat caatatgatt gaaaacgaca
    12541 agtacaatcc aaccttggaa ctctgtctca atctcgcccg cagcctccaa actgacctca
    12601 acagtctctt ttgggaggaa gatttttaaa aaaggagcaa actcatgaaa aaagaaacct
    12661 tcactgaaaa actgatcaaa cgcacatacg gtatttctgg tccccttgac gaatacaaac
    12721 ggcgtgaggc cgatagtatt gggaaccaag tctttatcat cctcttttat ctgatgattt
    12781 tcggaaatct tattccactc cttctggcct ataaataccc tcaagaagtg gctctaatct
    12841 atcctcctct gattttagtg attgccctca tcgcttctgg ctatgtcacc taccaaatga
    12901 aaaaaacagg catcacagtc attgaaccag atatgctgaa tgagaaagaa agcaagcaac
    12961 tacactaccc aggtctgaaa gcaggtttgt tctttggtct atggatgttt tttataactc
    13021 ctcttctcag gatactcata gatgaaggtc aggactattt tcattctctt ctcactataa
    13081 gaaatggtgt atcaagtatt ctcggttcta ttttcttcgg agcgagcata cagttcctca
    13141 tctcccgtcg cattgcaaaa actaagaaaa atcaagatga ggattaggag gaaactcatg
    13201 aaaaaagaaa agaaacagtt acgttatcct ggtctgaaag caggttcgat ttatggaaca
    13261 gtgatatttt ttataattcc actcattgat accctaacaa gtgaaaatcc aaattttatc
    13321 agttctcttc taaatacaaa acatattttt aaaactatac tgggagcttt ctttttcgga
    13381 gtgatgatac atattgtcga ctcacttcgt attgcaagag ctaaaaaaga ccaggattag
    13441 gaggtgcctt atgaaatcac tagctagact actgatcatt catgttttta tcagtatttt
    13501 ccttttcttc gcccttactt caggagctat ttctcataca gttttactac tcctactcct
    13561 ctttcttcct gcgctcaata aaggacttga gaaaatacaa tcaaaacgga tacctgtcct
    13621 caacgcagcc ctcttctttc tcctcatatc ctttccacaa cttttaacca accctgtcca
    13681 atggaaattt tcaatattcc tagtcgtaac catcatttca agtttggcct acttctataa
    13741 cttttatcaa gtagttaaag aagtagatca aaaacagttg atttaggagc ttgctcccat
    13801 aatttctcat ttcataggca aataatccca cctagcttgt tctaggtggg attattttat
    13861 ttcttaacag agattgcaag tcattttcct tcattttaga aagtcgactc tttccaattc
    13921 acactacaat ccaagacttg ttgcctttct tcttgattgc gaccttcaat ctggtcaatc
    13981 aaaatctttg tagcctgttg tccttcctca aaggagggct gaaccagcgt cgaaacactt
    14041 ggagaagaaa agcaagtcca ctccgtattg tcaaaaccaa tcaacccaac ttgtggcaag
    14101 ttataattca actctttgat aacggtaaag actagaggta gggcccaaca gttagggata
    14161 aataccagag ttttttcatc gggatcgatt tctttttgta aaaattcctt aatttgttcc
    14221 aaattcgtat gcttatcttc aatggttaga ctggcgtgac gcatattagc atctgttaaa
    14281 gcatccacaa aaccacttgc ccgctcaatc cgagtactca aacgactcgt atccgctgta
    14341 atcaagagaa aatgttcata acctttttcg atacaggact gggtcatgtc ataaacggca
    14401 tcatagttat tggttttaac ccagctagtc cggtgttcat agagctgact atcaaaaaag
    14461 accattttct tctttttctc atcgatgata cgagaatatt ttcggaaatt agaggtcggc
    14521 tgaataataa agccgtctac tcccaagaga agcatgcttt caatataccg gtcctcactc
    14581 tcttggctgt aattactatt tcctatcatt acctggtagc cattctggct ggcgatatcc
    14641 tcaattccct taacaatttg gtttgagaaa ctgttggtaa tatcaccaat caaaacaccg
    14701 attaattttg ttcgtttgga gtttaagcta cgcgcaacaa tgctcggttt gtaatttgtt
    14761 tcatgaataa ctttttcaat cttttcacgt gtctcttggg acattttttc atatttcccg
    14821 tttaggtaaa atgacacggt tgttttcgag gtctgagcca tttccgcaat gtcttttatg
    14881 gtcagtttct tctccaatct aaaacacctc ctactaatac ttattataac atattcacta
    14941 ttcaaaatgc ctatttctag cactgcaagc gaatcattct ctcatttatt ttatcatata
    15001 ctaaacattt tccacgcatt tttgttccgt caacgaggca caatttttca cctacaccaa
    15061 tatcctcgct taataacaga atcagcttgt ctgtttcatc atttttcact tcaaaagcca
    15121 gagcattttc cacttcacga tcatctgtct ggtagacaga atgacggatg atttcaaaac
    15181 tctcatgcgc aatgatggta taatccagca tctgattctc aaagaattgg cgcttggtga
    15241 gtttgctact tctttcaagc tcattgtatt taggagaaat gatggtatct tccaaatcaa
    15301 aatcaacttc actccatagt ctcaactgat tgattttccc atcttgatag gtcacatcct
    15361 tgtcaaggat aaactgagtc aacacctcat gttgaccttg acacctgatg tcatctacca
    15421 agagccagac atcctctacc aacatgagga tttttctcct gtgaagataa ggcaaatcag
    15481 gttctgctga ccaataagcc ccctcaatat aatgcactcc ctccctttct ttatggtgac
    15541 aaaacaggga gtgaggatag tattcatatt cccaggatcc cgtgattctt tccggagctt
    15601 tcccatctac aatgcaggtc gaatgactcc aagcactctt taagagataa cgttcatata
    15661 tctcccgata agaataacgc ccagcatcta tgaaaatagg ttggccttga tactgtaagc
    15721 aaaaactatt ctcgtcacta tgactatggg cacttcctag cggaccattt ttgaaaaata
    15781 gataacgatg ttcatcctta atgcagacat gtccagagtc ttcaaagatc atggacttag
    15841 gctgccaagc tctcttttca aattcctgca gtcgcttgac cttttctcgc cccaggaaca
    15901 agaggctaag caaatcaact ttaacatcca gaccgttaag aaggtcttcc tggttcaaaa
    15961 ccacagcaga caggctcaaa atttctgtcg tttctgtaga atcgctatca ccaaaagcca
    16021 aagtccgtcc atctaagcct gtcatcattt gaatataggt cgccatcttt tccagcaact
    16081 cttggtaact atcttgcaag tctggaagca agagacacaa atccagcaag gctttataaa
    16141 cctctacatg atagagaatc gactgttcaa actggcttcc atctcctaaa atctgtgtct
    16201 caatttgctg tttcaactcc tctgaagcaa aatggtaagc ttcttctaga tccatcttat
    16261 ctgaaaagaa atgatagata gcaagcatcg gaattgtttg taaaatcccc cagttactaa
    16321 gggtgtactt ggcgcgatag tagcttttca taaagtcaat ctgcttttct agactgacca
    16381 aaattttctc tagttctttc tcctctagca agtcaaattt caagaggagc aagagtagtt
    16441 tcaaccaagt aaaggaacga atacccgtat ccaaggttct agtcatcaag gattgaggag
    16501 aaaattctct cacctgctca atccaatcaa atagaaagaa cttgcacttt tgaatatagt
    16561 ccttatctcc ttctaccaga taccctatca taaactgcaa gagatattct tgtcgattga
    16621 gcatataaga ccattctgga tcatcttcaa atacttgatc ccataccatc ggctggattt
    16681 gatggatttt tgaacaaggc tccatatccc aaggactatc aaacataaaa cgattgtcca
    16741 tcaagcgttc aagggaactc ttgactttct catagtcttt tgaacagtgc gacaagatat
    16801 aatcacgaca ttgatttcca tcgactcttt caaaaaattg tcttctttct tctttcatta
    16861 tctattacca gaaaaagaac tacttaaaaa gcagttcttt tgtctttccc attacacttt
    16921 ccttttctac atggatgacc acaccttttg caatctgcaa ggagaccaag tcatcttgga
    16981 tagaaatgat ttttccatga attccagaca ataacaacac ttcatcacca aatgttaaag
    17041 aagctaaata ctcttgtcgt tgctccatct gtttgcgaag caacttttgc tgacgaatag
    17101 aatgaaagct tgacagtaaa aggggactca ctgccaagac aatcactatt ccataaaaca
    17161 atgttgtatc cattaagcta taatcttaag ccagcttccg ataattccga tgataactgt
    17221 taaaataacg agtttatatg ttgtccattt cttttctttg atcaagtagt aaactaaaag
    17281 tgtaaatagg gctggtagaa gagctggagc aaccttatca agcattccct gaatacttac
    17341 gatactttgt ttagcgtctg ctttaacttc ccctgcagca aaggtaatcg gcaccataat
    17401 cttaacagat gtcgctgcca aaccagcaat tacggttaca ccgataatat tggcaatacg
    17461 agaaatcgtt gccatctgtt cgcttagttt atcaatcaca gttgttccta gtttgtatcc
    17521 atacagacca gttgacaatt taatcgctgt taaaatcgta ttcatcgcaa ggaagaacaa
    17581 gattggaccg acaaccaagc cttcttgagc aaacgaagct gcgatggttg agaacaatgg
    17641 agctaaacag aattgagaaa gagaatcccc aatacctgcc aatggtccca tcaaggccat
    17701 cttgatgcta cgtgtttctt ttgccggacg gccattttcc aacattacaa gatgcaagct
    17761 ggtaataaaa ggcaggaagt gtgggttggt attatagaat tcacagtttt cttccaaggc
    17821 ttggtagaaa ccttcctgat cctctccata gtgttttttc aaagcaggat acatcacatt
    17881 ggcatatccc aacccttgat agttactata gttaaatcca ttttgacaaa agaatgcccg
    17941 caaagacgtt ttaagataat cacgttttgt taatttgtta gatccagtca tcgtgtgctt
    18001 cctcctctac cacatgatcc gctgtttttg gcttgttata aaattcaatc aaagcaaaga
    18061 tagtacctac aattgcaata ccaattgttg ggatgtttag ataagctgca caaacatatc
    18121 ccaacaagac aaagggaatc aactctttct tagccatcac tgacaagatc atcgcaaaac
    18181 cgatagctgg gagcatttta ccagcaactg tcaaacctgt aagtaatacc ggtggaatgt
    18241 agtctacgag tttcaacaag gtatccattg aaagggcacc caagcaaccc aaggtaaatc
    18301 caataaaggc aaacaaccaa attgttgcat ttagagtgaa cttaaatttc ttcaaattat
    18361 ggtttttcaa gtgcttcata gctgtttcag gcgcaccagc acgtacagtg taggcgaaag
    18421 tttgtaagaa ttgaatcgcc acagcaatcg gagtagagag ggcaagagcc gcttctggac
    18481 tgactttacc agcactagtg atagccatca aggtaccaaa gataccaggt ccgattgggt
    18541 ttggtggaac agtacctcca gcaccaacac cgaatcccat ataagccaat tcaccaatag
    18601 ctcccattgc aagagcagta ggcagatcac ctagaataat tccgacacca aatgacagaa
    18661 caatacatct attggtgtaa attcctaaca gcattccact aaaacagaaa gctgtccata
    18721 atccaattaa aatcgcttga aatacattaa tcgacataac gatctcctct tttaaatata
    18781 gtccataatg ttgacttgaa cagctccatc atttcctgtt ggagttgttt tcgtattaaa
    18841 tgttacttga tgagtttggc tcaattcctt gagggccgcc ttgtcctctt cacccaggaa
    18901 gatggagcgt gtcacttgct ctttaccagg ggcattgtga atgttcccaa tattgatttc
    18961 tttgatagtg acaccacctt ctaccaaggt taaagcgtcc ttcacatcct ttacaacgat
    19021 aaagatcgtt tgagcaggat tagccttgtg aatgatatca atcacctttt gcaaagggaa
    19081 gaaacgcatg gcaactgagt ctggcacaac tgttttcatc agagtttgtt gcatcttgtc
    19141 cgtgcttact tcgtcattgg caacaatgac cgtattacaa cctaggtatt ttacccaaag
    19201 ttgtccttgt ccatgaatca accgttcatc gatacgggtc ataataatat ttggcattgt
    19261 catatttctc ctcctaccaa tataggttcc agtctttgta gaaacggata agggcttcta
    19321 ggtaatagta gtcaccccag atattgcctt catccactcc tttacctgaa tgccatgagt
    19381 acacaccgtg gaggagactt gtcccaccag gggtaaattg atcatttgca taatgttcga
    19441 tcaaggaacg aagcatggca tgcatagcat gtttataaat atctttgtca gcatccacct
    19501 ctgggagatg ttttagcatt tcatgaatcc cacagacggc gatagctgtt gctgaagaat
    19561 ctcgtgattg atcactacca tcattaaaaa tcaaatccca ataggacaca tgatcttttg
    19621 gcagacgatt caagaaataa ttggtcacac ccttaaacaa gtcaaagcag gactcgtctt
    19681 ttaagtgacg ataagtcaaa ggaataccat agactcccca tgattgacca cgtgcccagc
    19741 atgaatcatc actataccct tgtctcgtta cacctttaaa gggttgacct gtctcaggat
    19801 caaaatagaa ggtgtggaag gacgaagcgt catcacggat tacattatta gctgaagcat
    19861 agaaatggct ttctgcaata tcgtagtatt tttgatcgcc tgtttcttga taagcaaaga
    19921 ataagagttg gatattgagc aagcagtcga taatcaaacg gtaatgctct ttcttgccca
    19981 agtctcccca agcttgaata aaaccacctt tttcttgata gcgttcaatc aacttatctg
    20041 cagctttcaa ggttgcttct ctagcctctc catctccatt tatcttatat tcagccatac
    20101 aagacggtgt gtacaagaag ccgagatcat ggtgatccaa ttctactctc ttattgacac
    20161 gatccaggaa agaaagaaca tttttatgag cgatgttttt aaatgcatcc tgttgactgt
    20221 attcataagc caaccacagt tctcctgtcc agaaaccatt ggtccattcc gtgttatcca
    20281 tgattggata gacattatca aaggtagctg gcgtcgggaa atcttccttg aaatagtcaa
    20341 ggttgagttc taactgccga ataaccttat cgattgcctg gccgacttct tctttcgtca
    20401 gaagtggtac ttctaagaag cgctcaggcg attttatttt ttcaatcgta acctttttta
    20461 tcattctttt accctttaaa tacccgattc gaaatcttct tcctcttcct ctgcatccgt
    20521 tgaaaacaat tctttaccat ttacgactcc gccctgggcc gccactactg atttattaac
    20581 aacctcatca aagctatgag ccattctagc aaagactgct tccattaaca tggctaagtt
    20641 caaacccgag agaacattca ttgtcttggc tggattttct cccattatgg tagaagaaac
    20701 cttgaatggc gatcctccca agagatcact taggattaaa acttcttctt catttgaaat
    20761 tgcaagtaag attttttgct tgagttcatc tgctgacatt ccttccacaa agtcaatcgc
    20821 ctccacattt tcttgattac ctgcaatcaa ttgtaaagaa ctataaatcc ctgtagcaaa
    20881 atgtccatgc cctacaagta caattttcat cttttctcct ttctttttag gtttaccggt
    20941 ttactttata ttccccattg taatccttta tttttatctt gtcaatagtt tttttaaaaa
    21001 aatattagc
//
LOCUS       DAVKNL010000033        20912 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVKNL010000033 DAVKNL010000000
VERSION     DAVKNL010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20912
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.033"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            98..2320
                     /gene="recJ"
                     /locus_tag="VW411_001256"
     CDS             98..2320
                     /gene="recJ"
                     /locus_tag="VW411_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEU9793983.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQNQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            2495..4156
                     /locus_tag="VW411_001257"
     CDS             2495..4156
                     /locus_tag="VW411_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU9793984.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            4225..5004
                     /locus_tag="VW411_001258"
     CDS             4225..5004
                     /locus_tag="VW411_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEU9793985.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALTTNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            5116..6336
                     /gene="murM"
                     /locus_tag="VW411_001259"
     CDS             5116..6336
                     /gene="murM"
                     /locus_tag="VW411_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEU9793986.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMIWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            6341..7573
                     /gene="murN"
                     /locus_tag="VW411_001260"
     CDS             6341..7573
                     /gene="murN"
                     /locus_tag="VW411_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEU9793987.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQDLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSDYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            7652..8353
                     /locus_tag="VW411_001261"
     CDS             7652..8353
                     /locus_tag="VW411_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEU9793988.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            8393..10237
                     /gene="uvrC"
                     /locus_tag="VW411_001262"
     CDS             8393..10237
                     /gene="uvrC"
                     /locus_tag="VW411_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEU9793989.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEAYFKSMAQEVSDFLKGQDDKIIDDLKSKMAVAAQSMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDSKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMKHFKSLTKIKEA
                     SVDEIVEVGVPRVVAEAVQRKLNPQGEALPQVAEERVDYQTEGNHNEP"
     gene            10227..11060
                     /locus_tag="VW411_001263"
     CDS             10227..11060
                     /locus_tag="VW411_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU9793990.1"
                     /translation="MNHKIAILSDVHGNATALEAVIADAKNQGASEYWLLGDIFLPGP
                     GANDLVALLKDLPITASVRGNWDDRVLEALDGQYGLEDPQEVQLLRMTQYLMERMDPA
                     TIVWLRSLPLLEKKEIDGLRFSISHNLPDKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSQGQQIINPGSIGMPYFNWEALKNHRSQYAVIEVEDGELLNIQFRKV
                     AYDYEAELELAKSKGLPFIEMYEELRRDDNYQGHNLELLASLIEKHGYVEDVKNFFDF
                     L"
     gene            complement(11242..12042)
                     /locus_tag="VW411_001264"
     CDS             complement(11242..12042)
                     /locus_tag="VW411_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9793991.1"
                     /translation="MKKFSLLLAILPFLVACENQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            12213..12818
                     /locus_tag="VW411_001265"
     CDS             12213..12818
                     /locus_tag="VW411_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU9793992.1"
                     /translation="MKFLELNKKRHATKHFTDKLVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYSEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDEKLEPSYRLPVDEIIEKR"
     gene            12831..14231
                     /gene="pepV"
                     /locus_tag="VW411_001266"
     CDS             12831..14231
                     /gene="pepV"
                     /locus_tag="VW411_001266"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEU9793993.1"
                     /translation="MTAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGVARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FHFDETSADNTIALNIRYPKGTSPEQIKSILENLPVVSVSLSEHGHTPHYVPMEDPLV
                     QTLLNIYEKQTGFKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            14285..14863
                     /locus_tag="VW411_001267"
     CDS             14285..14863
                     /locus_tag="VW411_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEU9793994.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQVLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            15023..15175
                     /locus_tag="VW411_001268"
     CDS             15023..15175
                     /locus_tag="VW411_001268"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9793995.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLEGAHNLPLSQ
                     LADSYD"
     gene            15218..15310
                     /locus_tag="VW411_001269"
     CDS             15218..15310
                     /locus_tag="VW411_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9793996.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            15481..16806
                     /gene="brnQ"
                     /locus_tag="VW411_001270"
     CDS             15481..16806
                     /gene="brnQ"
                     /locus_tag="VW411_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEU9793997.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            16904..17941
                     /locus_tag="VW411_001271"
     CDS             16904..17941
                     /locus_tag="VW411_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEU9793998.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREIADYLVKTLEGFGYQPVRTSK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKMDRIGGFPWNMIEGENCTI
                     HVASTGEKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDAKVTSEKETRALGIEVG
                     DFISFDPRTVVTETGFIKSRHLDDKVSAAILLNLLRIYKEEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVIATERM
                     VDAYLKSTLVD"
     gene            17943..18413
                     /locus_tag="VW411_001272"
     CDS             17943..18413
                     /locus_tag="VW411_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU9793999.1"
                     /translation="MCLICQRIDLIKKEENPYFVKELETGYLVVGDHQYFEGYSLFLS
                     KEHVSELHHLKKETRLRFLEEMSLVQEAVAKAFAAEKMNIELLGNGDAHLHWHLFPRR
                     TGDMNGHGLKGRGPVWWVPFEEMTAETCQAKPDEIKRLVKRLSSEVDKLLEIKE"
     gene            18416..19132
                     /locus_tag="VW411_001273"
     CDS             18416..19132
                     /locus_tag="VW411_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEU9794000.1"
                     /translation="MKKRYLVLTALLALSLAACSQEKTKNEDGETKTEQTAKADGTVG
                     SKSQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKAAADRYSARPGYSEHQTGLAFD
                     VIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            complement(19172..19309)
                     /locus_tag="VW411_001274"
     CDS             complement(19172..19309)
                     /locus_tag="VW411_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794001.1"
                     /translation="MKIKEQTRKLAAGCSKYSFEVADKTDEVSSKHCFEVVNRTDAVS
                     R"
     gene            19441..19866
                     /gene="rplK"
                     /locus_tag="VW411_001275"
     CDS             19441..19866
                     /gene="rplK"
                     /locus_tag="VW411_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEU9794002.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            20074..20763
                     /gene="rplA"
                     /locus_tag="VW411_001276"
     CDS             20074..20763
                     /gene="rplA"
                     /locus_tag="VW411_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEU9794003.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
BASE COUNT         6248 a         3872 c         4852 g         5940 t
ORIGIN      
        1 tactataaaa tgttataaaa aagcaatctg gatagagaaa acgtctaaat catgttataa
       61 tgaagcaata gaattcttag aaagagtgga tgtctttttg ataacaccta cttatgaatg
      121 gcagtttgcc ctgcaggtag aagatgcgga ttttacaaag atagccaaga aggctggact
      181 gggtcctgag gtggctcggt tattgtttga gagagggatt cagaaccaag aaagtctgaa
      241 gaagttttta gaaccttcct tggaggactt acatgatgct tatctgctcc atgatatgga
      301 caaggcagtg gagcggattc gtcaggctat tgaagaaggg gaaaatattc ttgtttatgg
      361 agactatgat gcggatggca tgacttcggc ttctattgtg aaggaaagtt tggaacaact
      421 tggtgctgag tgccgagttt acctgccaaa tcgttttacc gatggctatg gccctaatgc
      481 tagtgtttat aaatacttta tcgagcaaga agggatttcc ttgattgtga cggtggacaa
      541 tggggttgct ggtcatgagg ctattgcatt ggctcagtct atgggagtag atgtcattgt
      601 gacagaccat cattccatgc ctgaaaccct gccagatgct tatgctattg tccatcctga
      661 acatccagat gcggattatc cttttaaata tttggctggt tgtggagttg ctttcaagtt
      721 ggcttgtgcc ctgttagaag aagtgcaagt ggaattgctt gatttggtcg ctattggaac
      781 tattgcagat atggtgagtc tgacggatga aaatcgtatc ttagttcaat atggtctgga
      841 aatgttgggt catacccagc gcattggtct gcaagaaatg ctggacatgg ctgggattgc
      901 tgccaacgaa gtaacagaag aaacggttgg tttccagatt gctcctcgtt tgaatgcctt
      961 gggtcgcttg gatgatccca atcctgccat tgatttgttg actggatttg atgatgagga
     1021 agcgcatgag attgccctta tgattcacca gaaaaacgaa gagcgcaagg aaatcgttca
     1081 gtctatctat gaagaagcca agaccatggt ggatcctgag aagaaggttc aggtcttggc
     1141 caaggaaggc tggaatcctg gggttctagg aatcgtggct ggtcgtttat tggaagaatt
     1201 gggacagaca gtcattgttc ttaatataga agacggtcgt gccaagggca gtgctcgtag
     1261 tgtggaagcg gtcgatattt ttgaagctct ggatccccat cgagacctct tcatcgcctt
     1321 tggaggtcat gcaggtgcag cgggtatgac gctggaagtt gagcaactct cagatttatc
     1381 tcaggttttg gaagattatg ttcgtgaaaa aggtgcagat gctggtggca agaataagtt
     1441 aaacctagat gaagagttgg atttggaggc acttagcttg gaaacggtca aaagttttga
     1501 acgtttagct ccttttggaa tggataatca gaaacctatt ttttatatca agaattttca
     1561 ggtcgaaagt gctcgtacta tgggggcagg taatgcccat ctaaagctga aaatttccaa
     1621 gggtgaggcg agttttgaag tggtagcctt tggtcaaggc agatgggcga cagagttttc
     1681 tcaaaccaag aatctagagt tagcggttaa attgtctgtc aaccaatgga atggccaaac
     1741 tgccctccag ttgatgatgg tggatgcgcg agtggaaggt gttcaacttt ttaacattcg
     1801 tggaaaaaat gcagtcttgc cagaaggtgt tccagtcttg gattttcctg gagaactgcc
     1861 aaatcttgcg gctagtgaag ctgttgtcgt aaaaaacatt ccagaggata ttactcagct
     1921 gaagaccatt tttcaggaac agcatttctc tgctgtctat ttcaaaaatg atattgacaa
     1981 ggcttattat ctgacaggtt atgggactag agatcagttt gccaaattgt acaagactat
     2041 ttaccagttc ccagagtttg atattcgcta caagctgaaa gatttggctg catatcttaa
     2101 tattcaacaa atcttgctgg tcaagatgat tcaagtattt gaagaactag gctttgtgac
     2161 gataaaagat ggtgtgatga cagtcaataa agaggcgcca aagcgggaga taggagaaag
     2221 tcaaatttac caaaatctca aacaaaccgt taaagaccaa gaaatgatgg cgctgggtac
     2281 ggtgcaagaa atttatgatt ttttgatgga aaaagagtag aagttaggaa agagttggga
     2341 aatcaactct tttttgaaaa cagaccttca ttttgaaaat catcaaaaaa atggtataat
     2401 ggtaggaaaa gattcggctg aaagtatcag aacttttaga ataagagggt agaattgccc
     2461 tataatcaag ataaactaag attttggagg aaaaatgagt aatatcagtt taacaacact
     2521 tggtggtgtg cgtgagaatg gaaaaaatat gtacattgct gaaattggag agtccatttt
     2581 tgttttgaat gtagggttaa aatatcctga aaatgaacaa ttaggggtcg atgtggtgat
     2641 tccaaacatg gattaccttt ttgaaaatag cgaccgtatt gctggggttt tcttgaccca
     2701 cgggcatgcg gatgccattg gtgctctacc gtatctcttg gcagaggcta aagttcctgt
     2761 atttgggtct gagttgacca ttgagttggc aaagctcttt gtcaaaggaa atgatgccgt
     2821 taagaaattt aatgatttcc atgtcattga tgagaatacg gagattgatt ttggtgggac
     2881 agtggtttcc ttcttcccta cgacttactc cgttccagag agtctgggaa ttgtcttgaa
     2941 gacatcggaa ggaagcatcg tttatacagg tgacttcaaa tttgaccaaa cggctagtga
     3001 atcttatgca actgattttg ctcgtttggc agagattggt cgtgacggcg tcctggctct
     3061 cctcagtgat tcggccaatg cagacagcaa tattcaggtg gctagtgaaa gtgaagttag
     3121 ggatgaaatt acccaaacta ttgctgactg ggaaggtcgt atcatcgttg cagctgtttc
     3181 cagtaatctt tctcgtattc agcagatttt tgacgctgcg gataaaacag gtcgacgtat
     3241 cgtcttgaca ggatttgata ttgaaaatat cgtccgcaca gcgattcgtc ttaagaagtt
     3301 gtctttagcc aacgaaattc ttttgattaa gcctaaagat atgtctcgct ttgaagacca
     3361 tgagttgatt attcttgaga caggtcgtat gggtgagcct atcaatggac ttcgtaagat
     3421 gtcgattggt cgccatcgtt atgtagaaat caaggatggg gacctagtct atattgctac
     3481 ggctccgtct attgctaaag aagcctttgt tgcgcgtgtg gaaaatatga tttatcaggc
     3541 aggtggggtt gtcaaattga ttacccaaag tttacatgta tcagggcacg gaaatgtgcg
     3601 tgatttgcag ctgatgatca atcttttgca acctaagtac ctcttccctg tccaagggga
     3661 gtatcgtgag ttggatgctc acgctaaggc tgccatggca gttgggatgt tgccagaacg
     3721 catcttcatt cctaaaaagg ggacgaccat ggcttacgag aatggagact ttgttccagc
     3781 tggatcggtt tcagcaggag atatcttgat tgatgggaat gccattggtg atgttggaaa
     3841 tgttgttctt cgtgaccgta aggtcttgtc agaggatgga attttcatcg tggctattac
     3901 agtcaaccgt cgtgagaaga aaattgtggc tagggctcgt gttcacacgc gtggatttgt
     3961 ttatctcaag aagagtcgcg atattctccg tgaaagttca gaattgatta accaaacggt
     4021 agaagagtat cttcaaggag atgactttga ctgggcagat ctcaaaggta aggttcgtga
     4081 caatctgacc aagtacctct ttgatcaaac caagcgtcgc ccagccattt taccagtagt
     4141 catggaagca aaataatcgt tgaaataaac agagagaaag tcgagtttcg gctttttctt
     4201 atagaaaaat agaaggagaa aatcatggca gtgatgaaaa tcgagtatta ctcacaagta
     4261 ttggatatgg agtggggggt gaatgtcctc taccctgatg ccaatcgagt ggaagaacca
     4321 gagtgtgaag atattcccgt cttgtacctt ttgcacggga tgtctggaaa tcataatagt
     4381 tggcttaagc ggaccaatgt agaacgcttg cttcgaggaa ctaatctcat cgttgttatg
     4441 cccaatacca gcaatggttg gtacaccgat acccagtatg gttttgacta ctacacggct
     4501 ctagcagagg aattgccaca ggttctgaaa cgcttcttcc ctaatatgac gagcaagcgt
     4561 gaaaagacct ttatcgctgg tctttctatg ggaggctacg gctgcttcaa actggctctt
     4621 acgacaaatc gtttttctca tgcagctagt ttttcaggtg ccctcagctt tcaaaacttt
     4681 tctcctgaaa gtcaaaatct gggaagtcca gcctactgga gaggtgtttt tggagagatt
     4741 agagactgga caactagtcc ctattctctt gaaagtctgg ctaaaaaatc ggataaaaag
     4801 accaaacttt gggcgtggtg tggcgaacag gatttcttgt acgaagccaa taatctcgca
     4861 gtgaaaaatc tcaaaaaact aggttttgat gtgacctata gccatagcgc tggaactcac
     4921 gagtggtact actgggaaaa acaattggaa gtttttttaa caaccctacc aattgatttc
     4981 aaattagaag agagactgac ttagtttgaa cttcagcata gggggagtag aactaaaata
     5041 aaatatgttt tcactagact tttcaaacga aagtagtaga atagtaataa aatactggag
     5101 gaaagagagt aggaaatgta ccgttatcaa attggcattc ccacattaga atatgatcag
     5161 tttgtcaaag aacatgaatt agccaatgta ttacaaagta gtgcttggga ggaagttaag
     5221 tctaattggc aacatgagaa gtttggtgtt tacagggaag aaaaattact ggcgacagct
     5281 agtattttga ttagaactct tccgctaggc tataaaatgt tttacatccc aagaggacct
     5341 atattggatt atggggataa agaactcttg aattttgcca ttcagtctat taagtcctat
     5401 gctcgcagta agagagcggt ttttgtgact tttgacccaa gtatttgcct atctcaaagt
     5461 ttaatcaatc aggaaaagac agaatttcct gaaaatctgg ctattattga tagtttgcaa
     5521 caaatgggag taaggtggtc aggaaaaacg gaggaaatgg gagacaccat tcaacctcgt
     5581 attcaggcga aaatatacaa ggaaaatttt gaagaagata aactttccaa gtcaacaaaa
     5641 caggctattc gaacagcacg aaacaaaggg cttgagattc aatatggtgg actggaacta
     5701 ttagattcat tttcggagtt gatgaaaaaa actgagaagc gaaaagagat tcatttgagg
     5761 aatgaagcct attataaaaa attgttagat aattttaagg acaaggccta tatcaccttg
     5821 gccaccttgg atgtttctaa acgttcgcaa gagttagaag aacagttagc gaaaaataga
     5881 gccttggaag agacctttac tgagtcgact cgaacttcaa aagtagaagc gcagaagaag
     5941 gaaaaagaac gtttgttaga ggaattgacc ttcttgcagg aatatataga tgtaggtcaa
     6001 gcgagagttc ctttagcggc tactttgagt ttggaatttg gtactacctc tgtcaatata
     6061 tatgctggta tggatgatga ttttaaacgt tacaatgcac caattttaac ttggtatgaa
     6121 acggctcgct atgcctttga acgaggtatg atctggcaaa atttaggtgg tgttgaaaac
     6181 tctctcaatg gtggacttta tcattttaag gaaaaattta atccaacgat tgaagaatac
     6241 ttgggtgaat ttacaatgcc cactcatcct ctctatcctc tgttaagact tgctcttgat
     6301 ttccgtaaaa cattaagaaa aaaacataga aagtaagtat atggcactaa caacactcac
     6361 gaaagaagag tttcagactt attctgatca ggtttcttct cgttccttta tgcaatctgt
     6421 ccagatgggg gatttgctag aaaaaagagg ggctcgaatt gtttatcttg ctttgaaaca
     6481 agaaggagaa attcaagttg cagctctggt ttatagcctg cccatgctgg gtggtctgca
     6541 tatggaactc aattcggggc cgatttatac ccaacaagat gctcttccag ttttttatgc
     6601 agagttaaaa gaatatgcca agcaaaatgg tgtattagag ttgcttgtaa aaccctatga
     6661 aacttatcaa acttttgata gccaaggtaa tccaatagat gctgagaaaa aaagtattat
     6721 tcaagatttg actgatttag gttatcaatt tgatggctta acaacaggtt acccaggtgg
     6781 agaaccagat tggttatact ataaagattt aactgaatta actgaaaaga gtttgcttaa
     6841 aagttttagc aaaaagggta aacccttggt gaaaaaggct gaaacctttg gcattcggtt
     6901 gaaaaagtta aaacgtgaag aactatcgat ttttaagaat ataacaaaag aaacctctga
     6961 acgtagagaa tatagtgata aaagtttaga atattatgag catttttatg atacttttgg
     7021 agaacaagcg gagtttctca tagcaagctt aaatttttcg gactatatga gcaaattgca
     7081 aggtgaacaa agtaaactag aagaaaactt ggacaagttg cgacttgatt tgagtaaaaa
     7141 tcctcattct gagaaaaaac aaaatcaact gagagaatat tctagtcaat ttgaaacgtt
     7201 tgaagttcga aaagcagaag cgcgagactt gattgaaaaa tatggagaag aagatattgt
     7261 tttagctggg agtttatttg tttatatgcc tcaggaaacg acttatctct ttagtggttc
     7321 ctacactgag tttaataagt tctatgcccc tgcactgctt caaaaatatg ttatgttgga
     7381 aagcataaaa cgtggaatac ctaaatacaa cttcctaggc attcaaggga tttttgatgg
     7441 aagtgatggt gttttgcgtt ttaaacagaa ttttaatggc tatattgtac gcaaagcagg
     7501 tactttccgt taccatccat cgcctttaaa atacaaagct atccagttac tcaaaaaaat
     7561 agtaggacgt taagatgaaa aagtcagtat ttagatttct tttagcttct tttagtaaaa
     7621 taattcttat ttgctagaaa ggtggagaga catgcgctgg ctttttcgtt tgataggggc
     7681 tttcttttct tttgtgtggc gtttgttttg gcgtctggtt tggatagttg tgctcttatg
     7741 tgtgcttgct ttcggacttc tctggtatct gaacggagat tttcaaggag cgctaaagca
     7801 agcagaacgg tcagtaaaaa ttggtcaaca aagtattgac caatgggaga aaacagggca
     7861 actgcctaag ttaagccaga cagatagtca ccagcattct gaaggaaggt gggcacaggc
     7921 ctctgctcgt atttacctgg atccgcagat ggattcacgc tttcaagagg cttatttaga
     7981 agcaatccag aactggaatc aaactggtgc ttttaacttt gaactcgtga ctgagtctag
     8041 taaggcggat attacggcta cggagatgaa cgacggaggc actcctgtgg caggagaggc
     8101 ggaaagtcaa actaatctct taacagggca attcttgtcc gtaacggtgc ggttgaatca
     8161 ttattatttg tccaatccat actatggcta ctcctatgaa cgccttgtcc atacggcaga
     8221 acatgagtta ggtcatgcga ttggcttgga ccatacagat gagaagtctg tcatgcaacc
     8281 agcaggttcc ttttatggta tccaggaaga ggatgttgca aacctccgaa aaatatatga
     8341 gactagtgag tagggtacta tctttcccta ctttttttgc tataatggaa ctatgaacaa
     8401 cttgattaaa tcaaaactag agctcttgcc gaccagccct ggttgctaca ttcataagga
     8461 taaaaatggc accattatct atgtaggaaa ggctaaaaat ctgcgtaatc gagtacggtc
     8521 ctattttcgt ggaagtcatg ataccaagac agaagctctg gtgtctgaaa ttgtggattt
     8581 tgaatttatt gttacggagt ctaatattga ggcacttctc ctagaaatca acctgatcaa
     8641 ggaaaacaag cccaagtaca atatcatgct caaggatgac aagtcctatc ctttcatcaa
     8701 aatcaccaat gagcgctatc cacgcttgat tatcactcgt caggtcaaaa aggacggagg
     8761 tctttatttt ggaccctatc ccgatgtggg ggcagccaat gaaatcaagc ggttgctgga
     8821 tcggatattc ccttttcgta agtgtaccaa cccgccctct aaggtctgtt tttattacca
     8881 tatcggccag tgtatggccc acaccatctg taagaaggat gaggcttatt tcaagtctat
     8941 ggcccaggag gtgtctgatt ttctgaaagg tcaggatgac aaaatcatcg atgatctcaa
     9001 gagtaaaatg gcagtagcag cacaaagtat ggagtttgaa cgtgcggcgg aataccgtga
     9061 cctgattcag gctattggaa cgcttcgaac caagcaacgg gtcatggcga aagatttgca
     9121 aaatcgcgat gtctttggct actatgtgga taagggctgg atgtgtgtgc aggttttctt
     9181 tgtccgtcag ggtaagctca tcgagcgcga tgtcaatctc ttcccctact tcaatgatcc
     9241 agatgaggat tttttgacct atgtaggaca attctatcaa gaaaaatctc atctagttcc
     9301 caatgaggta ctgattccgc aggatattga cgaagaagct gtcaaggctt tggtggattc
     9361 caagattctt aagcctcaac gtggagagaa aaaacaactg gtcaatctag ccataaaaaa
     9421 tgctcgtgtt agtctagagc agaagttcaa tctgctagaa aaatctgtcg aaaagactca
     9481 aggagctatt gaaaatctag ggcgtttgct ccaaatcccg accccagtac gtatcgagtc
     9541 cttcgataac tctaatatca tgggaactag ccctgtttcg gctatggtgg tctttgtcaa
     9601 cggtaaaccg agtaagaagg attaccgtaa gtacaagata aaaacggttg ttggaccaga
     9661 cgactatgcc agcatgagag aggtcattcg cagacgctat ggtcgagtac agcgtgaggc
     9721 tttgactcct ccagatttga ttgtgattga tggggggcaa ggtcaagtca atatcgctaa
     9781 gcaggttatc caagaggaac tgggcttgga tattccaatt gctgggctgc aaaagaatga
     9841 taagcaccaa acccatgaat tgctctttgg agatccgctt gaggtggtgg atttgtctcg
     9901 caattctcag gaatttttcc tcctccaacg catccaagat gaggtgcacc gctttgctat
     9961 cactttccac cgccaactgc gctccaaaaa ttctttctca tctcaattgg atgggattga
    10021 cggtctggga cctaaacgca agcagaatct tatgaagcat ttcaagtctt tgaccaaaat
    10081 caaggaagcc agtgtggatg agattgtcga agttggggta cctagagtcg ttgcagaggc
    10141 tgtgcaaaga aagttgaacc cgcagggaga agccttgcct caagtagcag aagaaagagt
    10201 agattaccaa acggaaggaa accacaatga accataaaat cgcaatttta tcagatgttc
    10261 atggcaatgc gacggcgcta gaagcagtga ttgcagatgc taaaaatcaa ggggccagtg
    10321 aatattggct tctgggagat atttttcttc ctggtccagg cgcaaatgac ttagtcgccc
    10381 tgctaaagga ccttcctatc acagcaagtg ttcgaggcaa ttgggatgat cgtgtccttg
    10441 aggctttaga tgggcaatat ggcttagaag acccacagga agttcagctc ttgcgtatga
    10501 cacagtattt gatggagcga atggatcctg caacgattgt ctggctacga agcttgcctt
    10561 tgctggaaaa gaaagaaatt gacggattgc gcttttctat ctctcataat ttacctgaca
    10621 aaaactatgg tggtgacttg ctagttgaga atgatacaga gaaatttgac caactgctag
    10681 atgcggaaac ggacgtggca gtttatggtc atgttcacaa gcagttgctt cgttatggaa
    10741 gtcaagggca acaaatcatc aatccagggt cgattggcat gccctatttt aattgggagg
    10801 cgttaaaaaa tcaccgttcc cagtatgccg tgatagaagt tgaagatggg gaattactca
    10861 atatccaatt tcgtaaagtt gcttatgatt acgaagctga gttagaattg gccaagtcca
    10921 aggggcttcc ctttatcgaa atgtatgaag aactgcgtcg tgacgataac tatcaggggc
    10981 acaatctgga attattagcc agcttaatag aaaagcatgg gtatgtagag gatgtgaaga
    11041 atttttttga ttttttgtaa gagtttccta aaatagccaa tgcaaactaa aaaagcgatt
    11101 ggctggtcca atcgctttta gtatatctta tactcaatga aaatcaaaga gcaaactagg
    11161 aagctagccg taggttgctc aaagcacagc tttgaggttg cagataaagc tgacgtggtt
    11221 tgaagagatt ttcgaagagt gttattgtaa ctgagattga tctgggaggt aagaaccacc
    11281 tagataggta ttgctgagtt tttcaagggt tccgtcttga tagagttctt tgagcgcttt
    11341 atcaaattgc tctttaaact ctttttggtc gcttgagaaa atgatataat tgctggggct
    11401 atctgcagaa ggtaaatcaa cgactgagag gtctaaacca cggtccttga taatcttttg
    11461 aacggatacc ttgtcaaaaa ctaggaaatc aaactctccg ttagcaaggt ctaggattcg
    11521 tttaccaata tcctcaccag aaaaattaat tgtagcggga ttatcagtgt gtttctgatt
    11581 ccagttattg atgaattgag cgttagaagt tccggtatcc tcttgtgttg ttttaccagc
    11641 gatctggtca agagaagtca aaggattttt cttgttgctg acaaggacga ggggattgtt
    11701 ggaaattgga agcgagtaaa ggtatttttc agcacgctct tttgtgtaac tcaagttatt
    11761 ggccgcagcc tgatagtgac cagaatcaag tcctgggaag atgctctccc aggcggttct
    11821 ttggaattga atctcgtagt cgctgagttt ttcatctact gcctttaaaa cttcgatatc
    11881 aaagcctgtc agattgccct tgtcttcgta gtcaaatggt ggcacgtcgc cagctgtagc
    11941 aaggacgatt gtcttttgag cgctagtctc tttgggtgta gcttgattct cacaggcaac
    12001 caaaaatggt aggatagcta gtaataggct aaattttttc atactgtctc cattcaaatg
    12061 taaagtactt gctagtttat cagaaacttt cctgataaac caatatatat cttttatgtc
    12121 tgtgatagaa aaacttaatc atgaaaaatc cctgcaagct ctttcaaatt atggtagaat
    12181 ggaagcagta gaattagaaa aggaaatcga ttatgaaatt tcttgaatta aataaaaaac
    12241 gtcatgcgac taagcatttt actgataagc ttgttgatcc caaagatgtg cgtacggcta
    12301 tcgaaattgc aaccttggcg ccaagcgccc acaacagcca gccttggaaa tttgtggtgg
    12361 tacgtgagaa aaatgctgaa ctggcaaagt tagcttatgg ttccaatttt gaacaggtat
    12421 catcagcgcc tgtaaccatt gccttgttta cagatacgga cttagccaaa cgtgctcgta
    12481 agattgcccg tgttggtggt gctaataact tttctgaaga gcaacttcaa tattttatga
    12541 aaaatctgcc agctgagttt gcccgttaca gtgagcaaca agtcagcgac tacctagctc
    12601 tcaatgcagg tttggttgcc atgaacttgg ttcttgcatt gacagaccaa ggaattggtt
    12661 ctaacattat tcttggtttt gacaaatcaa aagttaatga agttttggaa atcgaagacc
    12721 gtttccgccc agaactcttg atcacagtgg gttatacaga cgaaaaattg gaaccaagct
    12781 accgcttgcc agtagatgaa atcatcgaga aaagatagaa agaagaaaaa atgacagcaa
    12841 ttgattttac agcagaagta gaaaaacgca aagaagacct cttggctgac ttgtttagcc
    12901 ttttggaaat caattcagaa cgtgatgaca gcaaggctga tgcccagcat ccatttgggc
    12961 ctggtccagt aaaagccttg gagaaattcc ttgaaatcgc agaccgcgat ggctacccaa
    13021 ctaagaatgt tgataactat gcaggacatt ttgagtttgg tgatggagaa gaagttctcg
    13081 gaatctttgc ccatatggat gtggtgcctg ctggtagcgg ttgggacaca gacccttaca
    13141 caccaactat caaagatggt cgcctttatg cgcgcggggc ttcggacgat aagggtccta
    13201 caacagcttg ttactatggt ttgaaaatca tcaaagaatt gggtcttcca acttctaaga
    13261 aagttcgctt catcgttgga acagacgaag aatcaggctg ggcagacatg gactactact
    13321 ttgagcacgt aggacttgcc aaaccagatt tcggtttctc accagatgct gaatttccaa
    13381 tcatcaatgg tgaaaaagga aatatcacgg aatacctcca ctttgcagga gaaaatacag
    13441 gtgttgcccg tcttcacagc tttacaggtg gtttacgtga aaatatggta ccagaatcag
    13501 caacagcagt cgtttcaggt gacttggctg acttgcaagc taaactagat gcctttgttg
    13561 cagaacacaa acttagagga gaactccaag aagaagctgg caaatacaag gtgacgatca
    13621 ttggtaaatc agcccacggt gctatgcctg cttcaggtgt caatggcgca acttaccttg
    13681 ccctcttcct cagccagttt ggctttgctg gtccagccaa agactacctt gacatcgcag
    13741 gtaaaattct cttgaacgat catgagggtg aaaatcttaa gattgctcat gtggatgaaa
    13801 agatgggtgc tctttctatg aatgccggcg tcttccactt cgatgaaaca agtgctgata
    13861 ataccattgc cctcaacatc cgctatccaa aaggaacaag tccagaacaa atcaagtcaa
    13921 tccttgaaaa cttgccagtt gtttctgtta gcctgtctga acacggtcac acgcctcact
    13981 atgtgccaat ggaagatcca cttgtgcaaa ccttgttgaa tatctatgaa aaacaaactg
    14041 gctttaaagg tcatgaacaa gtcatcggtg gtggaacctt tggtcgcttg ctagaacgcg
    14101 gagttgccta cggtgctatg ttcccagact cgattgatac catgcaccaa gccaatgaat
    14161 ttatcgcctt ggatgatctt ttccgagcag cagcaattta tgccgaagct atttacgaat
    14221 tgatcaaata aaacgataga agtctgagat cttatgcttg gacttctttt tggagggaaa
    14281 gtagatgtct caaatcgaaa gaatcaaaca ggctatcatg gcggattcgc agaatgccag
    14341 ctatacagag cgtggcattg agcctctctt tgcagcgcca aaaactgctc gcatcaatat
    14401 catcggtcag gctccgggac ttaaaactca agaagcaggc ctttactgga aagataaaag
    14461 tggtgaccgc ttgcgggact ggctaggtgt ggatgaagat accttttaca attcaggtta
    14521 ttttgctgtt ttgcctatgg atttctactt tccaggacat ggcaagtcgg gtgatcttcc
    14581 gcctcgtaca ggttttgcag aaaaatggca tccgcaggtc ttacaggaat tgcctgatat
    14641 tcagttaacc ctcttgattg ggcaatatgc ccaagcctac tatttacagg agaaaatcag
    14701 tgggaaggta acggagaggg tgaaacacta taaagactat ctgccagcct attttccgct
    14761 agttcaccca tcaccacgaa atcaaatctg gatggccaaa aatccttggt ttgaggcaga
    14821 agtagtgcca gatttgaaaa aaagaattaa aaccatttta tagtcaatga aaatcaaaga
    14881 gcaaactagg aagctagtcg taggctgctc aaagtacagc tttgaagttg cagataaaac
    14941 tgacgaagtc ggtaacatac gcacggtaag gcgacgctga cgtggtttga agagattttc
    15001 gaagagtatt agaagaaaaa gaatgaaaga aatagccttt gacgcatttt accagcttta
    15061 ccaaaacgac cagctttctt tagtggatgt gagagaagtg gatgagtttg cagctcttca
    15121 tttagaaggt gcccacaacc taccgcttag tcaattggct gatagttatg attaattgga
    15181 caaagatcgc ttgcattata ttatttgcaa atctggaatg agatcggcgc gtgcttgcca
    15241 attcctatta gaacaaggtt ataatgttat caatgtccag ggtggcatgt tagcctttga
    15301 agaactttaa aattttgcat ttctcctact tggtgtggac tgggtaggag agttttattt
    15361 ttagataatt cttattttta agaaaattga aaacatttaa tatttgcctc gtgatgcttt
    15421 tttcagactc ctaatcgtgg tatactaggt cagtatttta taaatatgaa ggagattttt
    15481 atggctaaaa aaggtaccct aacaggtttg ctcctgtttg gaatattttt tggtgcgggg
    15541 aacttgattt ttccgccttc tctaggtgct ctatctggag aacattttct tcctgccatc
    15601 gcaggttttg tcttttcagg cgttggtatc gccgtcttga cccttattat tggaacgcta
    15661 aatcctaaag gatatatcta cgagatttca acgaagatag cgccttggtt tgcgactctt
    15721 tacctctcag ttctttactt gtcaatcggt ccattctttg ctaccccacg tactgctaca
    15781 acagcttacg aagtagggat tagccccctt ttgtcggatg caaataaagg acttggcttg
    15841 attgtattta cggttctgta ttttgcggca gcctatttga tttcgcttaa tccatcaaaa
    15901 atcttagacc gcattggacg tattttaacg ccagtctttg caattttgat tgttatcttg
    15961 gtcgttctgg gagctatcaa atatggtgga acaagtcctc aagctgcttc agctgcttat
    16021 caagcttctg cctttggtac aggtttccta gaaggttaca ataccttgga cgcccttgcc
    16081 tcagtggcct ttagcgtaat cgcagttcaa accttgaaac aacttggatt ttcaagtaag
    16141 aaagaataca tttcaactat ttgggttgtt ggtatcgttg ttgcccttgc cttcagcgct
    16201 ctttacatcg gtttaggttt tcttggaaat catttcccag taccagctga agcgatgaag
    16261 ggtggaacac caggtgttta catcttgtca caagccactc aagaaatctt tggctcaaca
    16321 gctcaactct tccttgcagc tatggttacc gtaacctgct tcacaacgac tgttggtttg
    16381 attgtgtcaa cagctgagtt ctttaatgag cgcttcccac aaatcagcta caaggtttat
    16441 gcgacagcct ttaccttgat tggatttgct attgccaatt tgggtcttga tgcgattatc
    16501 aagtactcaa ttccagtact ggttatcttg tacccaatca cgattgctat cgttatgatt
    16561 gtcattgtca acaaatttgt tgccctttca aaaccaggta tgcagttgac aattgctgtg
    16621 gttacagtta ttgccattgc aagcgtacta ggaagctcgt ttaaggttga gtttcttgca
    16681 aatcttgtta gcgttcttcc ttttgccaag gcatctctcc catggttggt gccagccatt
    16741 gttggaatct tgctctcatt ggttctacca aacaagcaag aaagcgatgt ttttgaaatg
    16801 gaataatcac ttaaatcact tttgtagcca agtctacagg agtgattttc tttttttatc
    16861 cgatgataaa tgtgttataa taggtagcga aagaggtgaa gaaatgaatc aaacagtaga
    16921 atatatcaaa gaactgacag ccattgcgtc gccaacaggc tttactcgtg agattgcgga
    16981 ctatttagtc aagactctag aaggttttgg ttaccagccg gttcgcacat ccaagggcgg
    17041 tgtcaatgta actattaaag gtcaaaatga tgagcaacat cgctatgtga ctgcccatgt
    17101 agatacgctt ggtgctattg tccgtgctgt caaaccagac ggccgtctca aaatggaccg
    17161 tatcggtggc tttccttgga acatgattga aggagaaaac tgtaccattc atgtggctag
    17221 cacaggtgaa aaagtatcag gaaccatcct catccaccaa acttcttgcc atgtctataa
    17281 ggatgcagga actgcagaac gcacgcaaga caatatggaa gtgcgtttgg acgccaaagt
    17341 aactagtgaa aaagaaactc gtgctcttgg cattgaggtc ggtgatttta tcagttttga
    17401 cccacgaact gtcgtgacag agacaggttt tatcaagtct cgccatttgg atgacaaggt
    17461 cagtgcggcg attttgctca atctccttcg catttataag gaagagaaga ttgaattgcc
    17521 cgtaacaact cattttgctt tttcagtctt tgaagaagtg ggacacggtg caaactctaa
    17581 cattcctgct caggtagtag aatatctggc tgtggatatg ggagccatgg gagatgacca
    17641 gcaaacagac gaatatacag tgtctatctg tgtcaaggat gcttctggac cttatcacta
    17701 tgacttccgt caacatttgg tggctttggc gaaagagcaa gatattccat ttaagctgga
    17761 tatctatcca ttttatggtt cggacgcttc agcggctatg tctgcagggg cagaagtcaa
    17821 acacgccctt ctcggtgctg gtatagagtc tagccattcc tatgagcgta cccatattga
    17881 ctcggtgatc gcaacagaac gaatggtcga tgcttatctt aagagcacgt tggtggacta
    17941 atatgtgcct tatttgtcag agaattgacc tcatcaagaa ggaagaaaat ccttactttg
    18001 tcaaagagtt ggaaacaggc tatcttgtgg ttggagacca ccagtatttt gaaggctata
    18061 gtctctttct atccaaggag catgtcagcg aattgcacca tttgaaaaag gagacaagac
    18121 tccgttttct agaagaaatg agtttagtcc aagaggcagt tgccaaggcc tttgctgctg
    18181 agaaaatgaa tatcgaactg ctaggaaatg gcgatgctca tcttcattgg catctgtttc
    18241 cacgacggac aggtgatatg aatggtcatg gtctcaaggg tcgtggacca gtctggtggg
    18301 ttccctttga agaaatgaca gcagaaacct gccaagcaaa accggatgag attaaaagat
    18361 tagtcaaacg tttatcgtca gaagtagata aactattaga aataaaggag tagaaatgaa
    18421 gaaaagatac ctagtcttga cagctttgct agccttgagt ctagcagctt gttcacaaga
    18481 aaaaacaaaa aatgaagatg gagaaactaa gacagaacag acagccaaag ctgatggaac
    18541 agtcggtagt aagtctcaag gagctgccca gaagaaagca gaagtggtca ataaaggtga
    18601 ttactacagc attcaaggga aatacgatga aatcatcgta gccaacaaac actatccatt
    18661 gtctaaagac tataatccag gggaaaatcc aacagccaag gcagagttgg tcaaactcat
    18721 caaagcgatg caagaggcag gtttccctat tagtgatcat tacagtggtt ttagaagtta
    18781 tgaaactcag accaagctct atcaagatta tgtcaaccaa gatggaaagg cagcagctga
    18841 ccgttactct gcccgtcctg gctatagcga acaccagaca ggcttggcct ttgatgtgat
    18901 tgggactgat ggtgatttgg tgacagaaga aaaagcagcc caatggctct tggatcatgc
    18961 agctgattat ggctttgttg tccgttatct caaaggcaag gaaaaggaaa caggctatat
    19021 ggctgaagaa tggcacctgc gttatgtagg aaaagaagct aaagaaattg ctgcaagtgg
    19081 tctcagtttg gaagaatact atggctttga aggcggagac tacgtcgatt aatactcttc
    19141 gaaaatctct tcaaaccacg tcagcgtcgc cttacctact gactgcgtcg gttctattca
    19201 caacctcaaa acagtgtttt gagctgactt cgtcagtttt atctgcaacc tcaaagctgt
    19261 actttgagca gcctgcggct agcttcctag tttgctcttt gattttcatt gagtacaaaa
    19321 aataaacttt tctcttgcaa ttccagataa atagtgtata atggatgggt atgtgaaaaa
    19381 catacttgtg ggaggtaaaa atctctaatt accgccaaaa ccacaaagga ggatttaaaa
    19441 atggctaaaa aagtcgaaaa acttgtaaaa ttgcaaatcc ctgctggtaa agctacacca
    19501 gctccaccgg ttggacctgc tcttggtcaa gctggtatca acatcatggg attcacaaaa
    19561 gagttcaacg ctcgtacagc tgaccaagct ggtatgatca ttccagttgt tatctcagtt
    19621 tacgaagata aatcatttac tttcgttacg aaaacaccac cagctgctgt tcttttgaaa
    19681 aaagctgcag gtgttgaaaa aggatcaggt acacctaata aaactaaagt tgctacagtt
    19741 actcgtgcac aagtacaaga aattgcagaa actaagatgc cagatttgaa cgcagcaaac
    19801 gtagagtctg caatgcgtat gatcgaaggt actgctcgtt ctatgggatt cactgttgtt
    19861 gactaatcaa taacacagta gaaaatctca cagcagtcta ttagttgctt ttcatactag
    19921 gcaagtgact gaggcttgta cttgggtaca gcaagggagc ttaaggccgt aggaagagaa
    19981 aaatagtaga ctgaaaaccc gcaagacttc atcatttcga gaagtgacgt gggagatgaa
    20041 aatcgattga accacttaca aggagaatag aaaatggcta aaaaaagcaa acaacttcgt
    20101 gctgctcttg agaaaatcga cagcacaaaa gcatacagtg tagaagaagc tgtagcactt
    20161 gcaaaagaaa ctaactttgc aaaatttgat gcaactgtag aagttgctta caacttgaac
    20221 atcgacgtta aaaaagctga ccaacaaatc cgtggagcaa tggtattgcc aaacggtact
    20281 ggtaaaactt cacgtgttct tgttttcgca cgtggtgcaa aagctgaaga agcaaaagct
    20341 gctggtgcag actttgttgg tgaagatgac cttgttgcta aaatcaacga cggttggttg
    20401 gacttcgacg tagttatcgc tacacctgat atgatggctc ttgttggacg tcttggacgt
    20461 gtccttggac cacgtaactt gatgccaaac cctaaaactg gtactgtaac aatggatgtt
    20521 gctaaagcag ttgaagagtc taaaggtggt aaaatcactt accgtgctga ccgtgcaggt
    20581 aacgttcaag caatcatcgg taaagtatca tttgaagctg aaaaattggt tgaaaacttt
    20641 aaagctttca acgaaacaat ccaaaaagca aaaccagcta cagctaaagg aacttacgta
    20701 acaaacttga ctatcacaac tactcaaggt gttggtatca aagttgacgt aaactcactt
    20761 taatcagtag ttaaagtaat gtaaaaaagt tgaagacgct atgtctcaac tttttttgat
    20821 gtacgacggg catgttgtat agtagatgtg tactattcta gtttcaatct actatagtag
    20881 ctcagaagtc ggtacttaaa cgtgctatat ca
//
LOCUS       DAVKNL010000034        20864 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVKNL010000034 DAVKNL010000000
VERSION     DAVKNL010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20864
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.034"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(114..389)
                     /locus_tag="VW411_001277"
     CDS             complement(114..389)
                     /locus_tag="VW411_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU9794004.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(495..1334)
                     /locus_tag="VW411_001278"
     CDS             complement(495..1334)
                     /locus_tag="VW411_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9794005.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRAVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1476..2324
                     /locus_tag="VW411_001279"
     CDS             1476..2324
                     /locus_tag="VW411_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU9794006.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSGWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(2417..3334)
                     /locus_tag="VW411_001280"
     CDS             complement(2417..3334)
                     /locus_tag="VW411_001280"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEU9794007.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKNYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            3772..3977
                     /locus_tag="VW411_001281"
                     /pseudo
     CDS             3772..3977
                     /locus_tag="VW411_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(4288..4581)
                     /gene="rpmA"
                     /locus_tag="VW411_001282"
     CDS             complement(4288..4581)
                     /gene="rpmA"
                     /locus_tag="VW411_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEU9794008.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(4598..4942)
                     /locus_tag="VW411_001283"
     CDS             complement(4598..4942)
                     /locus_tag="VW411_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEU9794009.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            complement(4958..5272)
                     /gene="rplU"
                     /locus_tag="VW411_001284"
     CDS             complement(4958..5272)
                     /gene="rplU"
                     /locus_tag="VW411_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEU9794010.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(5308..5395)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(5454..6158)
                     /locus_tag="VW411_001285"
     CDS             complement(5454..6158)
                     /locus_tag="VW411_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEU9794011.1"
                     /translation="MGGKMRLLPIRKISRQSKRLALFLTFCAGYVDAYTFIVRGNTLV
                     AGQTGNVVFLSVELIKNNVSDVRDKVLTLLAFMMGVFLLTIYKEKLRIVKKPILSLIP
                     LAILSIIIAFVPQTVDNIYLVPPLAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLA
                     FGDYFRTKHTPFLREGFIFVSLLSSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLS
                     MLFASWQKKVKEKASF"
     gene            6560..6859
                     /locus_tag="VW411_001286"
     CDS             6560..6859
                     /locus_tag="VW411_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEU9794012.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
     gene            7040..8366
                     /locus_tag="VW411_001287"
                     /pseudo
     CDS             7040..8366
                     /locus_tag="VW411_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(8628..9602)
                     /gene="pta"
                     /locus_tag="VW411_001288"
     CDS             complement(8628..9602)
                     /gene="pta"
                     /locus_tag="VW411_001288"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEU9794013.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(9646..10542)
                     /locus_tag="VW411_001289"
     CDS             complement(9646..10542)
                     /locus_tag="VW411_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9794014.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEKGFETLEAIELPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            complement(10539..11357)
                     /locus_tag="VW411_001290"
     CDS             complement(10539..11357)
                     /locus_tag="VW411_001290"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEU9794015.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            complement(11341..12012)
                     /locus_tag="VW411_001291"
     CDS             complement(11341..12012)
                     /locus_tag="VW411_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEU9794016.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            12140..12709
                     /locus_tag="VW411_001292"
     CDS             12140..12709
                     /locus_tag="VW411_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEU9794017.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            12822..13781
                     /locus_tag="VW411_001293"
     CDS             12822..13781
                     /locus_tag="VW411_001293"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU9794018.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            13791..14906
                     /locus_tag="VW411_001294"
     CDS             13791..14906
                     /locus_tag="VW411_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9794019.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFVNNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            14911..15258
                     /locus_tag="VW411_001295"
     CDS             14911..15258
                     /locus_tag="VW411_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEU9794020.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            15266..15481
                     /locus_tag="VW411_001296"
     CDS             15266..15481
                     /locus_tag="VW411_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU9794021.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGKKVYQGLVGDLYRFLSHADFLHQN"
     gene            15564..16205
                     /locus_tag="VW411_001297"
     CDS             15564..16205
                     /locus_tag="VW411_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEU9794022.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            16223..16912
                     /locus_tag="VW411_001298"
     CDS             16223..16912
                     /locus_tag="VW411_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU9794023.1"
                     /translation="MKKPVIGITGNEKTHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(16909..17589)
                     /gene="radC"
                     /locus_tag="VW411_001299"
     CDS             complement(16909..17589)
                     /gene="radC"
                     /locus_tag="VW411_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEU9794024.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(17632..19923)
                     /gene="pcrA"
                     /locus_tag="VW411_001300"
     CDS             complement(17632..19923)
                     /gene="pcrA"
                     /locus_tag="VW411_001300"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEU9794025.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNSQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDSDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKPASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            complement(20114..20836)
                     /locus_tag="VW411_001301"
                     /pseudo
     CDS             complement(20114..20836)
                     /locus_tag="VW411_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT         6395 a         4391 c         3784 g         6294 t
ORIGIN      
        1 acaatatttt cggaacattc aactttttaa ctctatttat tactagattt cataattaaa
       61 aaacctactg accaagctag aaagcttgat acaataggct ttttaaagac tgattattta
      121 acagcgtctt taagagcttt accagctttg aatgctggta ctttagaagc tgcaattgtc
      181 atttctttac cagtttgtgg gttgcgacct ttacgttctg cgcgctcacg aacttcaaag
      241 ttaccaaaac cgatcaattg aactttttca ccagctgcaa gatagtcagc tactgctgca
      301 aatacagctt caactgctgc tgctgagtct ttcttagtca attctgtagc ttctgctact
      361 ttagcgatca aatcttgttt gtttgccatg ttaatgattc ctccaaataa tttctaatta
      421 acaaatataa tcatatccta aaatcccttt taggtcaagt taaaaacgct atttcttccc
      481 attttattta ttttttagga gtggtaacgt atcaaaatag cccaagcgtt ctcacccgtg
      541 tgagtttgaa taatggaacc cgtttccaaa acagaaattg gcttttcaac ataagcttgt
      601 aagctttctt tcatctcttt tgcccaatca tcactaccag aatatgaaat tccaatctct
      661 gctacagcac gttcagaaag cgatgttatc aactcatcta accatttttt aaatgtttta
      721 gttccacgac ctttaaccat tggctgcaat tcatggtctt tcatttgcat gacagcacgg
      781 atattgagaa gagagctcaa caagccagtt acacggctaa ttcgtccacc tttgacaaga
      841 ttttccaaag ttgaaacacc aatataaagc tctgtatggt ttttaacctc ttctacatga
      901 gataaaattg cctccatatc tttaccttct tgagctaact tcgcagcctc aacaacttgg
      961 aatttcaggg cttggtcagt gaaggaacta tcaacaacag tcacatctgc agtagatagg
     1021 ctagcacctt ggcgtgctgc ttctaccgta cccgaaagag catgggacat atgaatagca
     1081 agaatctggc caccatcttt gcataggtct tcaaaaatct cagcaaagac acctacaggt
     1141 ggctgacttg ttttcggaag attcttactt tcttgcatca actgaagaaa tttaccttct
     1201 tctttcaaat ccgcatcaga ataaacaaca ttatcaatca ttacagataa tggaacaatt
     1261 gtaatatcta attgctttac tagttcaggt tcaatagtaa cagatgaatc ggttacaatc
     1321 ttaatttttg tcatagtatc aatctttcta ttttaggatt cagattggtt tccttacttc
     1381 taattatatc aaaaaaaaga ttaaaaatcc taatggagtc aatcaaattt tccgtaaaat
     1441 ttgatataat caacttataa gaaaagaggt gtcctatgat taaaaaaatt taccccattt
     1501 ttaccatttt actaggtgct gctatttatg cttttggact gacttatttt gtagttcccc
     1561 atcatctctt tgaaggaggg gcgacaggca ttaccctcat caccttttat ctttttaaaa
     1621 tccctgtttc cctcatgaac ctgctgatta atattcccct tttcatccta gcttggaaga
     1681 tttttggagc caaatccctc tattctagtt tactaggaac cttagctttg tccggctggt
     1741 tagctttttt tgagcatatt ccccttcata ttgatcttca aggtgattta ctaatcacag
     1801 cccttatagc gggaatccta ttgggaattg gccttggaat tatttttaat gctggaggta
     1861 caactggcgg aactgatatt ctagctcgta ttctcaacaa atacactcat atatccatag
     1921 gaaaactgct ctttatctta gatttttgta ttctcatgtt gattctccta atcttcaagg
     1981 atttgagatt ggtttcctac acgcttttgt ttgattttat tgtttctcgt gttattgatt
     2041 tgattggtga aggaggatat gccggcaaag gctttatgat tatcacaaaa cgtcctgacc
     2101 aacttgctaa ggcgattaat gatgacctcg gaagaggtgt tacttttatt tctggtcaag
     2161 gctactatag taaagaaaat ttgaaaatca tctactgtat tgtcggaaga aatgaaattg
     2221 tgaaaacgaa ggaaatgatt catcgaatcg atcctcaagc ctttataact attacagaag
     2281 cccatgaaat cctaggagaa ggcttcacct ttgaaaaaga ataaaaagag gtaatgtcgt
     2341 gacctcaaaa gttagactaa atcatctatc ttttgggtta cagacaacct cttttttatt
     2401 ttatttactc aagctcttaa gaccaattcc gagttacttc ttcatcagcc tttaactgat
     2461 ccactaattg gtcaactgag tcaaatttgg tcatatctcg aatgcgatca agccaataaa
     2521 ccatgacggt ttccccataa atatcttgat taaaatcaaa aatattgact tcaaaacgtg
     2581 cttcttctcc atcaaaggtc acatttttcc cgacactagc catagcacga tacttctgtc
     2641 tttgaatctc aacatcaaca acataaacgc catctgctgg catataagta cggtctaaaa
     2701 gcactaaatt cgctgtcgga taaccaattg tacgaccacg agcattacca tgaaccacca
     2761 tacctcttga tggaagcggt gcccccaaaa gttttcctgc ttctttcaca tttccatcta
     2821 aaatagcttg acggatacga gttgaactaa tctttccttt ctcatcttct acaggtggaa
     2881 caatgataac ttctccatca aagtaattct ttaaatcttc tgctgttttt ttgtcagaac
     2941 caaatgtata atcaaaacct gcaacaataa ttttggcatt catagccttg atataagttg
     3001 caaagaattc ttgtgcagtg agactagcga attgactact aaaatcaagg agatataatt
     3061 cttctacacc ttcgcgcttt aattttcttt cacgttcagc agggttcaaa atatgcaaaa
     3121 acaaatctgg atgataaggc tctaaagcga tctttggaga ttcattaaag gtcataacga
     3181 cgataggcaa caaatccttt ctcgcagcct tgttggcaac acgaaataat tcttgatgcc
     3241 ccttatgtat gccatcaaaa tagccgagaa caacgactga atcagatggt gtgccaatat
     3301 ctttttggtt ttttatagga atagtaataa tcataaaata attatatcat agcgatagct
     3361 atttctggaa cagaaaatct gaaatgttgt ttttttcaca tgaagtgtac ctgttttcaa
     3421 aaagcacttt attctatcgt tgcttaacta tgaactttgc aatattcttc tcaaaaactt
     3481 gtaggacatc ttcaaaattt tgcaaggagt gattagactt gttcggtaac cataaagtgt
     3541 catactatgc ttatgtatga aaaagcaatg caactaactc ctgagaactt taaattacta
     3601 attggtgccg aaaaggtaga atttagaatc gaggtacacc tatggctgta aaatttacaa
     3661 aatgagacaa cttgggcaag atgtttgaag aatttcctaa actccctgat ttgaagcaag
     3721 tcactttccc taatgacaaa gaaaaaagcc aaaacagcaa agaaaaacta gatgactgct
     3781 ttccaacaac tcccatctag tgtgcttcag actgggctat ttttctctcc atctgttagc
     3841 ttggattctc agaccgtttc agctaaagaa tatcttttcc cttatcagaa ggaacggctc
     3901 aagccattca gacaagtgaa gggacgacaa gccaatattt gaaaccagat agcagttctt
     3961 atagtcaatt gaaataaaat ctgaagaaat cgagtaggaa actcatatca atgtttaaca
     4021 gtgttctatt ccagattcat actcaatgaa aattaaagtg caaactagga agttagccgc
     4081 aggctgtact tgagtacggc aaggtgaaat tgacatggtt tgaatttgat tttcgaagag
     4141 tatcaatcca ctatacatat taaaaagtat gggtaccaaa acaaaaaaac aggctctccg
     4201 aaaactcgga aagccttatt ttaagctgtt tctagcttcc tcacctttac atttcaaggt
     4261 tcgggataaa aggttcaatg gaccttttta tttagcgatt gggtaaacag aaacttgttt
     4321 tttatcgcgt cctttacgtt caaagcgtac tacgccttca actttagcga acaaagtatc
     4381 atctccacca cgaccaacgt ttacacctgg atagatgtgt gtaccacgtt gacggtaaag
     4441 gattgatcca cctgttacag tttgtccgtc agctgcttta gctccaagac gttttgcttg
     4501 tgaatcacgt ccgtttgatg tagaacctcc accttttttg tgggcgaaaa gttgcaagtt
     4561 gttaagagtc atttttaaca taatgttttc ctccgtgtta gttttctgtg ataactctgg
     4621 tttggacgaa ctcagaatag ttctccgata agtttgccat acctaagaaa aatgattcaa
     4681 agaataactg ggtcatttct ctctggtgtg aaggaagatc ttttggtatt tcaaccatca
     4741 gatagccacc ttcatcttcg tttaattcta ggattggttc atagcctgca aatttctcaa
     4801 tagaattgat aaagttaatg gcaagcgtag aaaccgatgc acacacgaca tctaagccgt
     4861 attcgccact ctcggcgtgt ccagtaattt ccgcactcct cagctcgcca tcttcggctc
     4921 tctcaaagac tgcctgtatc atgtgttctc cttaaaatta agcgttgatt gcgttgatga
     4981 caacttttgt atatggttga cggtgacctt gtttacggtg gctacctttt ttaggtttgt
     5041 acttgtaagt aaccactttc ttttgttttc cttgtttttc aacagttcca actacagtag
     5101 ctccagcaac aagtggagtt ccgacaacag tgttttcacc accaacaaga acaacttcgt
     5161 taaaagtaac ttcttgacca gcttcaacgt tcaatttttc aacgtaaact gcttgaccaa
     5221 cttcaacttt aacttgtttt ccgccagttt tgataattgc gtatgtgctc attatgcacc
     5281 tcctatgatt tttatgggtt tccccgattt tttgtgaaga ctcgcctagc atcgtgggac
     5341 gaaccactta aacttgaata atcaagcaac gatgttcgtg cggttgcaca ggatcgtgca
     5401 tagtcaactc ttcaagtata gcatatctcc tattttctta caagtaataa cacctaaaat
     5461 gaagcttttt cttttacttt tttctgccaa gaggcaaaaa gcatgctgag gtaaaaaacg
     5521 ctcatcataa taggaacacc aagaatggtc ttttcatgat agaaaatcgt caaataggct
     5581 gaaaagacaa cgccaaggac aaaactacta agcaggctaa caaatatgaa tccttcacgc
     5641 aaaaaaggag tgtgcttggt tcggaaataa tctccaaaag ccagcatggt ccgtttgata
     5701 ttccctgtca taaaagcgtt attataggca atacccgaca cttctccaaa agcagttgtc
     5761 accagtccca tacagaaggc caagggcggc actagataga tattatccac agtttgcggc
     5821 acaaaagcaa taatgattga taagattgcc aagggaatca aggacagaat aggttttttc
     5881 acaattctca atttttcctt ataaatcgtt aataaaaaga ctcccatcat aaacgctagc
     5941 aaggtgagaa ccttgtccct aacatccgaa acattatttt taattaattc tactgaaaga
     6001 aagacaacat ttccagtttg tccagctaca agggtattcc cgcgaacaat aaaagtgtaa
     6061 gcatccacat atccagcaca aaacgtcaaa aaaagtgcta accttttaga ctgacgtgat
     6121 atttttctta taggtaataa cctcatttta cctcccattg tattttctct tagaaatatt
     6181 gtaccatttt ctttctaaaa aatcgtaggc taccatttag attttactat tagcataaaa
     6241 ataataatag acaactattt atccaaaaat agatagatgt aacatgtttg caaacaaagc
     6301 atacgaacct ttagtaaaat catttccatg aaactagaat agagccctct tagcaaaaat
     6361 cattatttta atttatttct aatcactcct tgacataaat aactctcacc aataaaagac
     6421 tatgtcttaa aaaaatggta taataaaatc aatacttggg cttgatggct atgctactaa
     6481 taacaattag gagagaaaat caggcacttg ttaacaacaa ggattatccc cttgagatga
     6541 aaggaacttt agaaatctta tgatgaacat gcaaaacatg atgcgtcaag cacaaaaact
     6601 tcaaaaacaa atggaacaaa gccaagctga acttgctgct atgcaatttg ttggcaaatc
     6661 tgctcaagat cttgtccaag cgaccttaac tggcgataag aaagttgtca gcattgattt
     6721 caatccagct gtcgttgacc cagaggacct tgagactctt tctgatatga ccgttcaagc
     6781 catcaactct gctcttgaac aaatcgatga aactaccaag aaaaaactgg gtgctttcgc
     6841 tgggaaatta cctttctaaa aacaaggagc tagaacaatg cttgtcgata acaaaggcta
     6901 agaaaggtgc aaaaatgact ctataatatt tgtagtgggt aaatccccta tggatattat
     6961 ggagcctatt tttgtgtaga aaaaagtccc atatgaccta taatgaaaag cgacaaaaca
     7021 actcattaga aagaatcata tggaacaatt acattttatc acaaaattac tagacattaa
     7081 agaccctaat atccagattt tagacatcgt caataaggat acacacaagg aaatcatcgc
     7141 caaactggac tatgaagctc catcttgtcc tgagtgcgga agtcaaatga agaaatatga
     7201 ctttcaaaaa ccttctaaaa ttccttatct tgaaacaact ggtatgccta ctagaattct
     7261 ccttagaaag cgtcgtttca agtgctatca ttgttctaaa attatggttg ctgaaacttc
     7321 tatcgtcaag aagaatcatc aaatccctcg tatcatcaac caaaagattg ctcaaaagtt
     7381 aattgaaaag atttctatga ctgatattgc ccatcagctt tccatctcaa cttcaactgt
     7441 cattcgaaag ctcaatgact tccactttga gtgtaatttt agaaatctgc ctaagattat
     7501 gtcttgggac gttgaaacag tccggggagt gactgtttca atcgggagat ggagatgagc
     7561 tttattgcgc aagattttaa caatctcaat atcatcactg ttcttgaagg tagaacacaa
     7621 gctgtcatcc gagatcactt tcttaaatat gatagagccg tccgatgtcg cgtcaaaatc
     7681 attactatgg atatgtttag cccttactat gacttggcta aacagcttcg ctttcaaatt
     7741 tctaggctca ggctgaaaca gtctcccagg ctgtttcact cccgaatgct aaaatcgttc
     7801 ttgatcgctt tcacattgta caacatctta gccgtgctat gagtcgtgtg cgtgtccaaa
     7861 tcatgaatca gtttcatcga aaatcccatg aatacaaggc tatcaagcgc tactggaaac
     7921 tcattcaaca ggatagccgt aaactgagtg ataagcgatt ttatcgtcct atttttcgta
     7981 tgcacttaac caataaagag attttaaaca agcttttgag ctattcggaa gacttgaaac
     8041 accactatca gctctatcaa ctcttgcttt ttcacttcca gaataaggaa ccagagaaat
     8101 ttttcggact tattgaggac aatcttaagc aggttcatcc tatttttcag actgtcttta
     8161 aaaccttcct caaagataaa gaaaagatta tcaacgccct tcaactacac tattctaacg
     8221 ccaaactgga agcgaccaat aatctcatca aacttatcaa gcgcaatgcc tttggttttc
     8281 ggaactttga aaacttcaaa aaacggattt ttatcgctct gaatatcaaa aaagaaagga
     8341 tgaaatttgt cctttctcga gcttagcttt tcttcaaccc actacagttg acaaagagcc
     8401 aaaaaattct cttttcatac tcatttaaaa tcaaaaaggg aatttatcgt tttacggata
     8461 tgtatattac agatttctat aggtcgtgta tagacaggat atcatcgctt ggaagttttc
     8521 ctttttgaca ccaatcagta atttagttct caggaattaa tggcagtgct tttcgtacat
     8581 agtttcataa ctacagtata gcatagtata tcactttata gttttcacta ttgatgaact
     8641 gcttgagctg ctgtgatgag ggtcaacttg taaacatcat ctgcattaca tccacgagaa
     8701 agatcattaa ctggcttgtt taaaccttgc aaaacaggtc ctacagccgc aaagccaccc
     8761 aggcgttcag ccatcttgta accaatattt cctgcctcga tacctgggaa gatgaagaca
     8821 tttgcttgac cagctaccgt acttccagga gctttcagag ctgcagtttc aggaacaaag
     8881 gctgcatcaa attgcaactc accatcgatt tcaaggtcag gacgcaagtc gtgagcaatt
     8941 ttagttgctt caacgacctt atcaacgctt tcaccaaacc ctgaaccttt agtagaatag
     9001 ctcaacatgg caattttagg ttcgatgcca aacatcttag ctgtgattgc tgagttgatg
     9061 gcaatttcag ccaaggcttc tgcatctgga ttgatgttaa tggcacagtc tccaaatagg
     9121 taacgttccg taccacgaac catgaggaag gctcctgaag tacgagttac atttggacga
     9181 gttttgatga tttgtagagc tgggcgaact gttgaagctg ttgagtgaat cgctcctgac
     9241 accattccat caaccaagcc caagtaaacc aacatcacac caaagtagtt gacatcttca
     9301 accaaaacct tgcgtacatc ttcttcagtc attttgccct tgcgacgctc caccaaggca
     9361 gaaaccattt cttcaaattg aggataatgt tgagggtcga tgacctcata accatccatg
     9421 attccttcaa tttcaagata aattttaatt ttttcaggat ttccaagcaa aacaggaatc
     9481 acttctgttt cttttactaa gcgttttgtt gcttgaagaa tacgaggctc ttccccttca
     9541 gggagaacga tacgagcatt tttaccaaca aggttggctt tgagactttc aaaaacttcc
     9601 atgagttttc tcctttaaga taataattat actctgagac agtttttata gagtattagt
     9661 tgataactgg gtaataaggt tactgaaatc atccggcaag ggactttcta actgcaagtc
     9721 ttgctctaaa aatggatgat aaaaggatag gtaatggcaa tgcagagcct gacgttgaat
     9781 accatcttcc agactaccac catacaaatc atctcccagc aaaggaaaac cgatatgaga
     9841 aaaatggact cggatttgat gggttcgacc agtgtgcagg tgaatataga ccaagtgaat
     9901 atttccataa gaagctacaa tcttgtatga agtatgggca tactttccgc ctttagccac
     9961 tcgtctggta ataatggaat cttcatcacg cgcaatcgga gcaataattt ccccttctgg
    10021 ctccaaatgt ccatctccct taaccaaagc aaagtagcgt ttctcgatag atttcttctg
    10081 caactgcttg tctaatcgtg catgggcata accgtgcttg gcaaagagca tcaagccaga
    10141 agtatcccta tctagtctgg taacaatgtg aacctgctga ttttcataat tttgcttgac
    10201 atagtaaccc ttgataaaat tggcaatggt attagagtga ttgacactag gaatagaagc
    10261 cactccatag ggtttattca agactagaaa gtggtcatcc tcatagagaa tatctaatgg
    10321 aagctcaata gcctccaagg tttcaaagcc tttctcagcg ggaatgtcaa tggtaacgta
    10381 gtctccaacg tccaatagat acgttgcatt ttgcggttga ttattgacca gaatagctcc
    10441 acctcgaaac ttaatcttgg ccagcaatcc cttagaaacc tcgtgctttt ttaagaaggt
    10501 cttaaccttg acatgttcat ctgcgataaa ttcaaacctc attcatccac ctcgccgata
    10561 aaggcgtcct taacacggtt ccagaaactg gtatggctag gagtcgcgac aaagtgaatc
    10621 ttatgatggt cgatttgata ctcaatacgc tcaatattac ggaaagaata aacgctattg
    10681 tcaaccgaaa tagtatgata atcgtttctt gttggaataa gttcaatctt atccttctta
    10741 ggcacaataa tggaagagcc cagtgttcga tagacacgat tattaaggct ggcaatttcc
    10801 gttaattgca aagcttcaat ggtagggtgt aaaacagcac cgccaagaga cttgttatag
    10861 gcagtactac cagtcggtgt cgaaactgtt agcccgtctc cacgaaaacg ttcaaaggga
    10921 acaccattta ttacaatatc tgccaccatg gttcgatcag acctgcggat gctggcttcg
    10981 ttgagtgctc tgaaaatctt aacttcacca ttttcaagaa agaccttcac attcagaaca
    11041 gggtaagaaa cccttgcccc agtatctagc tgcaaattag tcactagctt gtccaactca
    11101 aaatcacgat aatctgtata gaagcccaaa tgtccagtat gaagaccgat aaagcggacc
    11161 ttgtcaagct gattttcgta cttatgaaag gccgacaaga gcataccatc cccgccaatg
    11221 gaaatgacaa tatccggatt ggtatcattg agtataaact gatttctctt caaacgatct
    11281 cgcaattcat acaaaaccct ttgactctgc ggttttctat tggctatcag atcaattcgt
    11341 ttacctgtat tcttcatctg tatcgtcact gtttcctaca ccgtcattta attttctact
    11401 caaaggatca aaaagtgcct gggcttcttg gatatcatca cgaatttcac ccatttcttc
    11461 atccaactga tgggcgattc tagctgtaat ttccagtcgc ttcttaatct catctgggaa
    11521 atccccttgg tacttgtagt tgagagaatg ttctatcgtt gcccagaaat tcatggccaa
    11581 agtacgaatt tgaatttctg ccaaaatagt cttagctcca ttgatggtat caaccgtata
    11641 ttctactacc acatgatagg aacgatagcc tgatgctttt ctatgagtaa tgtaatctcg
    11701 ctcctgtatg attcgcatat cctgacgctt gtgcaaaata tccactactt ccttgacgtc
    11761 atctacaaac tgaaccatca cacgtaagcc agcaatatcc tgcaaatcgt gttccaaggt
    11821 cgcataagta atgccacgac gagccatttt ttctttgatg ctctcaattg gcttgactcg
    11881 accggtcaca aactcaattg gagaatgctt attttgctta cgatattgct tacgaatacc
    11941 acgaagttta atctttaact caccaacagc ttgaatgtaa ggatctagaa attcttccca
    12001 ttctaaggtc atatattctc ccttgttctc atattatccg tcaaaaatat ctttgatttt
    12061 ttctccagat tcatatacaa taaatacaat gcttattata ccataaatcc gctttcaacg
    12121 ataaaaaaag gtaagaaaaa tgaaacattt agaaattgaa ttgaaaacac tattgaaaaa
    12181 agatgaatac aatcgtctaa aagaccagtt cacaggtgtc actcctgttc ttcaaacaaa
    12241 ttactacatc gacacgcctg attttgaact gcgagaaaag aaagtcgcta tgcgcattcg
    12301 aacctttgaa gactgggctg agttgacact caaagtcccg cagagtgttg gaaacatgga
    12361 atacaaccaa aaattgcaac taaaagatgc tgagaactat ctgagtaagg aagaacttcc
    12421 tcaagggcta gtactggatg aattggcgaa acatggtatc caaagtaaga actggcaggt
    12481 tcttggttgt ctaacaacgc ttcgctatga aatgaaaaca gctattggtc tcatggcact
    12541 ggatcagagc cgatactttg atataacaga ttacgaattg gagcttgaag tggaaaatca
    12601 tgagcaaggc aaacaggatt ttcgacaatt tttagagaaa aatcagattt cctatcaaaa
    12661 agctccttca aaattggttc gatttgtcaa aagtatgaaa aatagctgaa ataatctcca
    12721 ttttttggta aaatagaaaa gataaaaata cacaaatccc agttcatata tgtaaggtaa
    12781 atataactag gatttataaa gtttacagag gacggtctat aatgtcagat agaaaaaaca
    12841 tgaaactttt cgcactcaac tctaaccaag agattgcaca aaaaattgcc caagctgttg
    12901 gtgtcccact tggaaaacta tcatcacgtc aattttcaga cggagaaatc caagtaaata
    12961 tcgaagaaag tgtccgtggt tatgatgttt acatcatcca atcaacaagt ttccctgtca
    13021 acaaccacct aatggaattg ttaatcatgg tcgatgcttg tgtgcgtgca agtgcccaca
    13081 gtatcaacgt tgtccttcca tattttggct atgcacgtca agaccgcatt gcttgtcctc
    13141 gtgagccact tacagcaaaa ctagttgcca atatgctggt taaggctgga gttgatcgta
    13201 tcctgactct tgatttgcat gccgttcagg ttcaaggttt ctttgatatt ccagtggata
    13261 atcttttcac tgttccccta ttcgcaaaac attactgcga taaaggattg cttggttcag
    13321 atgttgttgt cgttagccct aaaaattcag gtgtcaaacg tgcgcgtagc ctggctgaat
    13381 atcttgatgc tcctatcgcc attatcgact accctcaaga cgatgcaact cgtaacgaag
    13441 gttatattat tggtgatgtt gaaggtaaga aagctatctt gattgatgat attttaaata
    13501 caggacgtac cttctctgaa gcttctaaaa tcgttgaacg tgaaggagct acagaaattt
    13561 atgctgtttc tagccacggt ctcttcgtcg agggagctgc tgagcttctt gacaatacta
    13621 atattaaaga aattcttgtg actgattcag tagcaacaaa agaaaaaact cctaaaaatg
    13681 tatgctacat cactgctagt gagttaattg gtgatgctat cgtccgtatt cacgaaagaa
    13741 aaccagtcag cccactcttt gcctacaata aaaagaaata aggtgattct ttgatttatt
    13801 tggacaatgc tgcaacgact cctatgtcag cagttgctat ttcagctatg accaaggtta
    13861 tgcaagaaac ccacggaaat ccttctagta ttcatggtca tggtcgtcaa gctggcaaac
    13921 tcttgcgaga agcccgtcag gaactagccc agttactaag gacaaaacct caacatatct
    13981 ttttcacttc tggtgggact gaaggcaata atactaccat cattggctac tgtcttcgtc
    14041 accaagaaca aggaaaacat atcatcacaa ctgccatcga gcaccatgct gtccttgaaa
    14101 caattgatta cttggttcaa cactttgggt ttgaagcaac cattatccag ccagaaaatc
    14161 aagaaatcac agcccagcaa attcaaaagg ctttacgtga cgatacgatt ttggtttcta
    14221 ccatgtttgt caataatgag acaggaaacc tactgcccat cgctgaaatt ggccaaatac
    14281 tcaagcaaca ccctgctgcc tatcatgttg atgcagttca ggctattggt aaaatcccaa
    14341 ttcattcaga agaattgggc attgattttc tcactgcttc tgcccacaaa ttccatggtc
    14401 ctaagggaat cggttttctc tacgcatcta gcatggactt tgattcctat ctacatggcg
    14461 gagaccagga acagaaaaaa cgtgcaggaa ctgaaaatct gcctgccatt gtaggcatgg
    14521 ttgcagccct aaaagaagac ctagaaaaac aagaagaaca ttttcaacat gtacaaaatc
    14581 tagaaactgc ctttctggca gagctggagg gcattcagta ttacctgaat agaggaaaac
    14641 atcatctccc ttatgttctc aatattggat ttcctggtca gaaaaatgac ctcttactcc
    14701 ttcggctaga tttagctgga atttcaatct ctactggctc agcctgtact gcaggcgttg
    14761 tccaatccag ccatgttctt gaagccatgt atggcgcaaa ttcagaacgc ttgaaggaat
    14821 cccttcgcat cagtttgtcg ccacaaaata ccgttgaaga cctacaaacc ctcgcaaaaa
    14881 ccttaaaaga aattatcgga ggttagccat atggcatttg aaaaaatcat tcagttaaaa
    14941 aattgtcgtt acgattacac tcttagccct tctgttaaaa aattcaccct caaagataac
    15001 accttttttg aaactaaggt tggtaactat gaactgactc gccttttgga aaaagtgcca
    15061 aacagcggtg aaggcttcca actcaaaatc atcattaaca aggaacttac aggggctaaa
    15121 atcaatatca ctgacaagtt tggccttcgt ctagttgata ttttcaaatc agaagaccac
    15181 catattcatc aggaaaaatt ctacttcctc atggatagct tggtagaacg tggtgtcttt
    15241 acaaaatcgg aaagatagag ccaaaatgtt tgaactgacc tataaggact gctatcatgt
    15301 agagcgtact ctcaagtatg aggatcatga ggctctcatg ttgactttgt caggatgtgt
    15361 aaccttacca gatacacttt atgtgacttc tctgactttt aggggcaaaa aagtttatca
    15421 ggggctggtc ggagacctct accgttttct atctcatgca gattttttac atcaaaacta
    15481 agatttttct tagttttttc ttatttttgt tgattttttc acaatgtttc tataaaatag
    15541 aagaatagaa aggttgtgat tttgtgaaag ataaacagtt tgctattcca aaagctacag
    15601 caaaaagact ctctctctac tatcgaattt ttaagagatt tcatgcagaa aagattgaac
    15661 gtgccaactc taagcaaatt gcagaggcta tcggtattga ttcagcgacc gtacgtcgtg
    15721 atttttccta ttttggtgaa cttggtcgtc gtggttttgg ctatgatgtc aaaaaactaa
    15781 tgacattttt tgccgatttg ctcaatgaca actctattac caatgtcatg ctggttggta
    15841 ttggaaatat gggccatgcc cttctccact accgcttcca cgaacgtaac aagatgaaga
    15901 ttatcatggc ctttgaccta gacgaccatc ctgaagtcgg tacccaaact cctgacggga
    15961 ttcccattta cgggatttct cagatcaagg ataaaatcaa ggatgctgat gtgaagactg
    16021 ctatcctaac tgttcccagc gtcaagtcac aagaggttgc taatctcttg gttgatgctg
    16081 gtgtgaaagg aattctcagt ttttcaccag tccatctgca tttaccaaaa gacgtggtcg
    16141 ttcagtatgt cgatttgaca agtgaactcc aaaccctcct ctacttcatg cgaaaagagg
    16201 attagaaagc gaaaatcatt ttatgaaaaa accagttatt gggattacag gaaacgaaaa
    16261 aactcatcca gatgatgaca tcatgatgag ctacgcagca aaaggctttg ttgaaggcgt
    16321 taaagatgct ggagggattc ccatcatcct accgattggt gatcaagaaa tggcctgcca
    16381 ctatatcagt ttgattgaca agctcatctt gacaggtggg caaaatgtcg atccaaaatt
    16441 ttatggcgaa cccaaaacca ttgatagtga tgactatcac cttcaaagag atatcttcga
    16501 actggccctc atcaaggaag ctattaaaca gaaaaagccc attttctctg tctgccgtgg
    16561 tacccaactc tttaacgttg ccatgggtgg aactttgtac caagatatcg aagaccattg
    16621 gcaggattct tctgttgagt acacaactca acgcttggta acagaaccag ataccgttct
    16681 ccaagaaatc tatggagaaa tctctcatat caactccttc caccaccaga gcatcaagga
    16741 cttagcacca aatttaaaag ttgtggctca tgatcctaaa gatggtatca ttgaggctgt
    16801 catgagtacg gatgatgtcg cctttctcgg tgtccaatgg catccagaac ttctttttga
    16861 aaatcgtccc aaagataaaa aactctttga ctatgtcgtt aatgaacttt agattaaatc
    16921 tgtcttttca cgataactaa agtaattaga atgagagaca atcaaatggt ccaagagaac
    16981 aatccccatc aattcgcagg cttctttaac aagtttagtg acatgatcat cattttggct
    17041 aggcgctacc gctcctgaag gatgattgtg gaccaagata agagaagtcg ccatatgctt
    17101 gattgcatag tgaagaatct ctcgcggttc agcgatacta cgagttacag acccgataaa
    17161 aatggtctgc tgatggatga tttgattttg agtattgaga tagagtgcca ccaggtgctc
    17221 ttgtttttta tcccctaatt cctgctgcat cttcttggcc aacttttgac tgctgagaat
    17281 actttccatt tcaagagtct cgtgtttgtg aatacgatgc cccagttcaa tcatagcttg
    17341 taattctatg gccttaacac gcccaatacc agacaaactc tgcaattcct gcagggtcat
    17401 ttttttcaaa tccgttaggc ttgaaagatt gttcaagact ttttgggcaa tttcaaaaac
    17461 gctagcttga cgtgttcctg tcctgagtaa aatagctagc aactcttggt tactaagcgc
    17521 ttcaactcct tccttggcca gcctttctct tggtaatagt gaatcttctt ggaatgaaat
    17581 actgtacata aaaatcctcc tcactttatt attcgtgaga aggatggaaa attagatttt
    17641 tttctcaatt ggagccacac tggctaaaag ttttttcaaa cctacttctg ggaaattgat
    17701 tttcaattcc tgcctagcac cgctacctga aacttccaga acggttccct ctccccattt
    17761 cttgtggaga gcaatatcac caatggacca atttgcctcg ctagatgctg gttttgcgcc
    17821 agctgtaaat tgaccaaatg gaagaccgct tgactggatt gattttgggg cagcaccgcg
    17881 tttacggtct tgaagagcct gagccaaact cataccttga ccaaaggaaa tactaccact
    17941 gctatatgat gccttaaagc ttgtatttgc aggacgagcc agaccttgat actcaagcaa
    18001 gtctgaactg atttcgttaa taaaacgagt cggacggtta taattggtac gaccaaaaag
    18061 caagcgtgag ttggcattgg tcagatagag aattttctct gcacgcgtga tacctacata
    18121 ggctagacgg cgctcttctt ctaattcatc tgaatcttca gtcgcacgac taagtggaaa
    18181 gacattttct tccatcccaa tcaaaaagac aactggaaat tcgagacctt tggcagcatg
    18241 cagggtcatc aaggtcactt ctgatgtctc ctgactacct gaatctgtgt cggcaatcaa
    18301 agccaagtca tttaagaaac gactcagttt gtccagacca gtttcctctt ctgtcacatc
    18361 cgtggtgtca tcaaagttct tcgtaacaga aagaaactct tcgatatttt caacccgtgc
    18421 cttgctttct agagtcgctt gggagttaag aatatcgaca taacctgttt tttctaggac
    18481 ggactcaacc aactctgtaa tgcttaagtg gtctagctgc tcccgcaaat caagcatcat
    18541 attggcaaaa tcccagatag attgggctgc cttacccttg ataccagaca acataatatt
    18601 agcagaagca tccagcatag acatattttg caaatttgca aaatcacgga ttttctcaac
    18661 tgtacctaga ccaattccac gtttaggctc gttgataata cgctcaaaac taatattgtc
    18721 actcaaatta gcaataaggt tgagataagc aataatatcg cgaatttcct tacggctgta
    18781 gaatttggtt ccgccaacca tggtataagg aatgttagac ttgagcaggg cttcctcaat
    18841 tgtacgggac tgggcattag tccgatagag aactgcaaaa tccttatgaa ggaagttttg
    18901 actgcgacta agttcatcga tggttctggc tacaaataca gcctcatcca gctcatcatc
    18961 ggcacgatag taaacgattt gctccccatc agcgttttga gtccagagat ttttaggacg
    19021 gcgattttta ttatttttaa taacctcgtt ggccgcttgg agaatggttt tggttgagcg
    19081 gtaattttcc tccaacaaaa caaccttggc tttggggtaa tccttttcaa agtccaagat
    19141 attctgcata tcagcaccac gccaaccgta gatagactgg tccgcatccc caaccacaca
    19201 gatattttta aaacgggaag ccaagagttt gaccaattgg tactgagcgt ggttggtatc
    19261 ttggtactca tcaacgtgga tgtattggaa tttttgctgg tagtaggtca aaacatcagg
    19321 attttgatca aagagacgca aggtcagcat aatcaaatca tcaaagtcaa cggattcaga
    19381 ctgacgaagt tctttttgat aggctgtata acactgggcc acaatttgcg tatacatatc
    19441 gccagcttgg gcagcataag caacatcatc aatcaaatca ttcttagcat tggaaatggt
    19501 ccccaaaata gttcgttcat tccatttttt agggtccaag ttcaactgtt tgagaatacg
    19561 tttcatgagc gttcgctgtt caccaggatc cacaattgta aaattacgat tgtagccaat
    19621 atggtccgca tcgcgacgca aaatacgcac acacatggag tggaaggtcg caatcagaca
    19681 gtcctgagtc gctggattga ggctataagc acgctctttc atctcacgcg cagccttgtt
    19741 ggtaaaggta atggccaaga tattccaagg attgaccagc ttttcatcaa tcaaataagc
    19801 gatacggtgg gtcaaaacac gagtctttcc agaaccagcc cctgccatga ttagcaaggg
    19861 accttctgtc gtttgcaccg cctcagcctg acggtcattc attccattta ataatgcgtt
    19921 catcttctct tccttactct tgaagtcaat atttctatta tatcagaaat cagggaaaat
    19981 agaaacttta cggggagtag caaaatatag tggattgaaa ctagaatact acactgtggg
    20041 tttctaaaac actcgacttt tcacccaatt ttttcataaa ctagctcaat acctcaatag
    20101 acttcctgca aaactagaat cctagttcat gattgataat accagcactc aaattcattc
    20161 gtaatccgaa gcgtttacga tgatttcgat aggttgttga aaatatttta aacgttttta
    20221 ctttggcaaa gatgttctca accttgcttc tttccttaga tagcgcatgg ttataggctt
    20281 tatcttcagc tgttagcggc ttgagtttgc tggatttacg tggagtttgt gcttgaggat
    20341 atatcttcat gagcccttga taaccactgt cagccaagat tttaccagct tgttcgatat
    20401 ttctacgact cattttgaac aacttcatat catgactata gttcacagcg atatccaaag
    20461 aaacaattct cccttgactt gtgacaatcg cttgagcctt catagcgtga aatttctttt
    20521 taccagaatc attcgctaat tgttttttta gggcgattga tttttacttc cgtcgcatca
    20581 atcattaccg tgtcctcaga actgagagga gttcttgaaa ccgtaaaacc actttgaaca
    20641 agagttattt caacccattg gctccgacgg ataaagttgc tttcgtgaat accaaaatca
    20701 gccgcaattt cttcataagt tcgatattct cgcactattg aagagtgggc ataagaaggt
    20761 cttctaggct taatttaggt tttcgtccac cttttgcgtg tttaagttga taagctgttt
    20821 ttaatacagc taacatctct tcaaaagtgg tacgctgaac acca
//
LOCUS       DAVKNL010000035        20389 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVKNL010000035 DAVKNL010000000
VERSION     DAVKNL010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20389
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.035"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            16..739
                     /locus_tag="VW411_001302"
                     /pseudo
     CDS             16..739
                     /locus_tag="VW411_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(728..1374)
                     /locus_tag="VW411_001303"
                     /pseudo
     CDS             complement(728..1374)
                     /locus_tag="VW411_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            complement(1483..2001)
                     /locus_tag="VW411_001304"
     CDS             complement(1483..2001)
                     /locus_tag="VW411_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEU9794026.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            complement(2228..3097)
                     /gene="rsmI"
                     /locus_tag="VW411_001305"
     CDS             complement(2228..3097)
                     /gene="rsmI"
                     /locus_tag="VW411_001305"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEU9794027.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGL
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQGVEEKDEEDLFVEIQTRIQQGVKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            complement(3100..3417)
                     /gene="yabA"
                     /locus_tag="VW411_001306"
     CDS             complement(3100..3417)
                     /gene="yabA"
                     /locus_tag="VW411_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEU9794028.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            complement(3457..4347)
                     /locus_tag="VW411_001307"
     CDS             complement(3457..4347)
                     /locus_tag="VW411_001307"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEU9794029.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFESLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQGEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            complement(4344..4982)
                     /locus_tag="VW411_001308"
     CDS             complement(4344..4982)
                     /locus_tag="VW411_001308"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEU9794030.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            complement(5098..5895)
                     /gene="proC"
                     /locus_tag="VW411_001309"
     CDS             complement(5098..5895)
                     /gene="proC"
                     /locus_tag="VW411_001309"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEU9794031.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSELFYQLLAKAGLLVELGES
                     LIDAATGLAGCGPAFVYLFIEALADAGVQTGLPREIALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMDAVHQAYKRTQELGK"
     gene            complement(5899..7161)
                     /locus_tag="VW411_001310"
     CDS             complement(5899..7161)
                     /locus_tag="VW411_001310"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEU9794032.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASFLPRLEQ
                     VLVAERKEAGLEPIQFRLDSKASQFVSGQAAETQDFDTEFLDYVLAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            complement(7171..8301)
                     /locus_tag="VW411_001311"
     CDS             complement(7171..8301)
                     /locus_tag="VW411_001311"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEU9794033.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQYGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            complement(8381..10264)
                     /gene="cbpE"
                     /locus_tag="VW411_001312"
                     /gene_synonym="pce"
     CDS             complement(8381..10264)
                     /gene="cbpE"
                     /locus_tag="VW411_001312"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEU9794034.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRKDGFVNISTSYKPIPS
                     FQAGWHKSAYGNWWYQAPDSTGEYAVGWNEIEGEWYYFNQTGILLQNQWKKWNNHWFY
                     LTDSGASAKNWKKIAGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYMGQW
                     YYFAPSGEMKMGWVKDKETWYYMDSTGVMKTGEIEVAGQHYYLEDSGAMKQGWHKKAN
                     DWYFYKTDGSRAVGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVSIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNFSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            complement(10267..11154)
                     /locus_tag="VW411_001313"
     CDS             complement(10267..11154)
                     /locus_tag="VW411_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9794035.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLALAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            complement(11144..11605)
                     /locus_tag="VW411_001314"
     CDS             complement(11144..11605)
                     /locus_tag="VW411_001314"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEU9794036.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            complement(11602..12510)
                     /locus_tag="VW411_001315"
     CDS             complement(11602..12510)
                     /locus_tag="VW411_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU9794037.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKSQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLREGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            12503..12700
                     /locus_tag="VW411_001316"
     CDS             12503..12700
                     /locus_tag="VW411_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9794038.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSSQPY
                     FQNQFKKITGYTPLQYRKEKR"
     gene            complement(12752..13423)
                     /locus_tag="VW411_001317"
     CDS             complement(12752..13423)
                     /locus_tag="VW411_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9794039.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRFFNYLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETVEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(13477..13698)
                     /locus_tag="VW411_001318"
     CDS             complement(13477..13698)
                     /locus_tag="VW411_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HEU9794040.1"
                     /translation="MILMTKNINLTNEELELIQGGADPYGKNPNGRYDWEIEPVLTLL
                     VHGFCPRGTYDSGYIGGGNHLCKGSAARF"
     gene            13970..14779
                     /locus_tag="VW411_001319"
     CDS             13970..14779
                     /locus_tag="VW411_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9794041.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLELDLQNEYIIVNNGCSTHQTSDWSLVDWKELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGDKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYIKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(14960..15835)
                     /gene="aguB"
                     /locus_tag="VW411_001320"
     CDS             complement(14960..15835)
                     /gene="aguB"
                     /locus_tag="VW411_001320"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEU9794042.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(15845..16930)
                     /gene="aguA"
                     /locus_tag="VW411_001321"
     CDS             complement(15845..16930)
                     /gene="aguA"
                     /locus_tag="VW411_001321"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEU9794043.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAELVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEKRYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(16927..18054)
                     /gene="nspC"
                     /locus_tag="VW411_001322"
     CDS             complement(16927..18054)
                     /gene="nspC"
                     /locus_tag="VW411_001322"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEU9794044.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDADLEELLEIMDHIVF
                     NSERQLRKHGPRCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFESQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(18054..19313)
                     /locus_tag="VW411_001323"
     CDS             complement(18054..19313)
                     /locus_tag="VW411_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEU9794045.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVKKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            complement(19310..20170)
                     /gene="speE"
                     /locus_tag="VW411_001324"
     CDS             complement(19310..20170)
                     /gene="speE"
                     /locus_tag="VW411_001324"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEU9794046.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
BASE COUNT         5939 a         4685 c         3642 g         6123 t
ORIGIN      
        1 acgacttttg aagagatatt agctgtatta aaaacagctt atcaacttaa acacgcaaaa
       61 ggtggacgaa aacctaaatt aagcctagaa gaccttctta tggccactct tcaatatgtg
      121 cgagaatatc gaacttatga acaaattgcg gctgattttg gtattcacga aagcaactta
      181 atccgtcgga gccaatgggt tgaagtaact cttgttcaaa gtggtgttac gatttcaaga
      241 actcctctca gttctgagga cacggtaatg attgatgcga cggaagtaaa aatcaatcgc
      301 cctaaaaaaa gaattagcga atcattctgg taaaaagaaa tttcacgcta tgaaggctca
      361 agcgattgtc acaagtcaag ggagaattgt ttctttggat atcgctgtga actattgtcg
      421 tgatatgaag ttgttcaaaa tgagtcgcag aaatatcgga caagctggta aaatcttgtc
      481 tgacagtggt tatcaagggc tcatgaagat atatcctcaa gtacaaactc cacgtaaatc
      541 cagcaaactc aagccgctaa cagctgaaga taaagcctgt aaccatgcgc tatccaagga
      601 gagaagcaag attgagaaca tctttgccaa agtaaaaacg tttaaaatgt tttcaacaat
      661 ctatcgaaat catcgtaaac gcttcggatt acgaatgaat ttgattgctg gcattatcaa
      721 tcatgaacta ggattctagt tttgcaggaa gtctaatgta attctcaagc tgtgagttca
      781 ttatgatttg taaatccgat atatcttcag cagctaaaaa cacattttga aatgttccaa
      841 gtccgttttc accaaaacta tattctctct tactatactt actcttatta ttctctatat
      901 agttagagtc aaggttctta acctcttgaa gttcattttc tttacttctg aggttaaaat
      961 cttttacttc tgaagttaag ttttttgact tctgtaagtc attttctttt acctgaaata
     1021 tactcataaa gtctttaaca taaatgatgt tcggcttacc aagtccaagc cttactcttt
     1081 cgatcagtcc tattcctttt ttacatcaag ctcatctaat gtttttatgg cagttggctt
     1141 tgagatattt cttcttttca taatttcttc gacagtaaaa tagataaata ctctgccttc
     1201 cttgtctatc cagttattct taaatgacat tcctgtctgt ttaagaagta tggaataaag
     1261 gataattgct tctgctgata agccccgaaa ttcttctcta tcaactaata tctccggtac
     1321 ttttaagaag ttaaatcttt ccgcttctct gttataaaaa tattcaaagt ccatgcaaga
     1381 tcacctccct tcttaaaaat ttgcaaagaa aaagacgata gtttttcttc tatcgccttt
     1441 ggtaaccatt tttatgaaat tttttaaagt taaaaatata atttataata ctatcttctt
     1501 ccattttctg attttagttc tgaaagttcc aaaaggtgca accgtattta catgaataaa
     1561 cttatacact tcccaagtcg ctgttttagt cgcttcatca gcccactttc tcatatgcgg
     1621 ttcaaataat tcttcctcac tcaacgaatc aatcattgca gagatagaat taatattttc
     1681 atttaatttt gctttcaact cttgcagaga taaatgagcg taggtatctg tgaaccactg
     1741 atataattca ccaagttgat tccatttaaa tttatccgat ggtgttttta cttgaagccc
     1801 ctttctttca tcttcttccc atttaagaac caagttggtc caaccaacct gataagcaag
     1861 gttttctgct ggagttctgt caacttcatc agctctctta tcttttaaat tttctggaat
     1921 attatcaaat tctaaaatat atttctcaaa tgttttctct atctcagctt ttagttcttc
     1981 tttattttcg tatgttcgca aaaaatcacc ttctttctga tttatcaatc ctttgattta
     2041 aaatgacctt atttttctat ttataccttc taagaacgct ataactgctg ctccaaccat
     2101 cggtattccg tatgggctta ataaaaaact taataccaaa gcttctatac caagtgatac
     2161 atctttatat tatcatcact aattaagtta aacagaaaca gatagaatta ttacatcctg
     2221 tctcccttta ttgtttttct tcccagtcgt ggtaggcagc gtagagctga cttttattcc
     2281 actggtaaat cttagcgact tccttgatag cttggttttt cttcacacct tgctggatgc
     2341 gggtttgaat ttctacgaac aagtcttcct cgtccttttc ctccacaccc tgactggcac
     2401 cctcaacaat gagaagacat tcgcccttga gtggcgtttc agcaatgctt tctaataact
     2461 cagagatagt acctcgttgg tattcttcat agattttggt caattccctg accaagacaa
     2521 cggagcggtc accgtagact tctaacatat tttccaacgt gtctgctaca cgatgaggtg
     2581 attcataaaa aatctgtgtt tcaggataat cttttttcaa gccaaaaaat tgcttctgct
     2641 gacctgattt tctcggtaaa aaaccgtaaa agatatgtgg ctgtggcgct aaaccactgg
     2701 caatcaaggc agaaattcct gcagaggcac ctggaactgt cacaactgca atttcttcct
     2761 caatagctgc cttaactaaa tcatgaccag ggtctgaaat gctaggcaaa ccggcatcag
     2821 agacctgagc aatactttgc cctgctttca agaaaccaat caaatcagga attttttcct
     2881 tggcattgtg ctcatgaaaa ctgatctgct tggtggaaat gtcaaaatgc ttgagcaaaa
     2941 gccctgtatt gcgcgtatcc tcagcagcaa tccagtccac ttctttcaag gtctggatag
     3001 cacgaaaagt catatcatct agattgccaa tcggcgttgc cactagatac agcttgccat
     3061 agggagactg ccccttaaaa cttttttgaa tctgcatgcc tactccctgt atagcaactc
     3121 gtcacaaaac atacattcct cgtcctgctc tcgacgttgt ccataaaaat cattacatac
     3181 gtgaaatcca tcacggtaaa tgcgacggac actttcacga acatgcttgg ccttgacagg
     3241 agcatctgct tccacctcac ccaagcgttc tcgcaactta ctattttcca agcgaagagc
     3301 tgtattttcc tctaccaggc tcttgagatt tttcttgatg gcttccacat cggctaaggt
     3361 taccaataat tgttgggaaa aatcatccag cgcgtcaaat aattcttttt tgtccataaa
     3421 acagcccttt cctttcttta tcattcattt ttgagtttat atttctttca agaccagata
     3481 ttccatggca ttttgaaagc tgacattagc ttgccacatt tttctagctt ctagtaaatc
     3541 ttgtagaatc actcttactc ttacctgcaa gaggtcctgc ccacagagaa cttcaagaat
     3601 ccgtaaaacc tgatcctgtt tttccttatc atctgccaag ttggctaatt tggcaacctg
     3661 tagataactt tcttttttct tagctactaa ccaagtcagc aggcgttcac tttcatcgac
     3721 caaggtccaa aaacttgcct gattagccaa cttttctgct tcagctcgcg attgactaaa
     3781 cttagctaaa agagtcgctt ttttcttaac aagtcccatt tgttctaaga gtaagataag
     3841 tttttcttct tgctttttaa agtggaagat ctgagtccga cttcggattg tcggtaacat
     3901 cttttcctca tcgctagtca agaagaaaat ataaacttca ctctggggtt cttcgatgac
     3961 cttgagcaga gaattggctg cgttgggatg cattttatcc gcttgctcga tgataaagac
     4021 ctgttgctgg ctttcaatcc ctgcttgaga aaactgaccc accaattctc gaatgcgttc
     4081 cgtcttaatg acctgattaa ctggtttaat caaggtgaca tcgggaaatt ctccctgttc
     4141 aatcagcttg caacttcggc atttctcaca tggtaagacg ccaactttat ccgtacaaaa
     4201 gaggctctta gctaaaaatt gcgccatttc caagctttca aagaaacctg aaaagagata
     4261 ggcgtgattg agctggtctt gttctaagat acggacaaaa cggtcaaact gagctggttg
     4321 ccaagccttt agttgatctt gtttcatttg gccaagccca ttccgtcaaa caagacagcc
     4381 ttggtagttt ccacaacttg ctccaaaggg agactagcat caatcttgac aatgcgattt
     4441 ccctctttat ccagaagaga aaggtagcct tgacgaactt ttttatgcaa gtccaaccct
     4501 tccaaatcca aacgattaac ctcgcggtca ctattagcag caatacgagc cagcccttct
     4561 tccacctcga tgtcaaaata gagtgtcaaa tcgggtttga ggccatctgt cgcaaactga
     4621 ttgagccagt caatggcttc aatatctaag ccacgaccaa atccctgata ggcaacagaa
     4681 ctatcgataa aacgatccat gatgaccaac ttgccagctt caagggctgg aagaactttt
     4741 tccaccaaat gctgtctgcg actggcaata tagagaagta gctctgtttt agcatccatc
     4801 tgagtatgac ttggatccaa aatcacttcc cgaatcttct ccccaatcaa gactccgcca
     4861 ggttcacggg tcgtcaacac ctctactcct ttttcctcta aaattggtag cagagcctct
     4921 aaaacactgg tcttgcctgc tccctctggt ccctcaagag agactaaaaa tccttttgac
     4981 atgtctaact catttctttt ttactacttc tattctatca aaaaaatagg ggtttgtgac
     5041 aatctttttg tgtcttctca tttcagccac cataaaagag gcagtcaaaa ctacctctta
     5101 tttacctagt tcttgtgttc gtttgtaggc ttgatgaact gcatccatga ctgttcctcg
     5161 gaaagcatgc gcttctaggc ttgctacacc agcgatagtc gaaccgcctg ggctacagac
     5221 ttggtctttc aatactccag gatgttgctg actttcaagg accaattgcc cagctcctac
     5281 cacagtttgt gctgccattt tcaatgctat ttctcgtggt aatcctgtct gaacacctgc
     5341 atctgccaag gcctcgataa aaagatagac aaaggctggt ccacaacctg caagacctgt
     5401 cgctgcatcg attaaacttt ctcctagttc aaccaagaga ccagccttgg ctaaaagctg
     5461 ataaaagagc tcactgtcct cagccctgca attaggagac aaggcataac taatcactcc
     5521 ttgcccgata gaagcagggg tattaggcat catacgaata attcggtgtt gacttgggat
     5581 aagacttgct agtttttcta aggtcaatcc agctgccatc gaaatcaaaa gaagactttc
     5641 tcttttttca aggatggtct ggtattgaga aagcagttca gaaaactgag caggcttaac
     5701 tcctagaaaa atcacatctg cttctgcaaa catttcttca ttgctggaag cctgaccacc
     5761 aaagtctgca atgaaagcat ctaccttagc ttgactacga ttggcaagga gaatctcatc
     5821 tgacgtccta gtctgcaaga cagattttgc caagctagca cccatattcc ccaaaccgat
     5881 aaatccaatc ttcatctctt actcccttat ctgcccatca ccggcaacca catacttgta
     5941 gctggtcaac tctttcaagc ccatgggacc acgcgcgtgc aatttctgag tagaaatccc
     6001 catttcacaa ccaagaccaa attgtcctcc atctgtgaaa cgagttgagg cattaacata
     6061 caccgctgca gagtccactt gatctgtaaa gtatgctgca gcttcagcat tttccgtcac
     6121 aatagcatcc gaatgatggg tgctgtggga ttcaatgtgc gcaaccgctt cttctaaact
     6181 gctcacaacc ttaacagcaa ggacatagtc taaaaactcg gtgtcaaagt cttgggtctc
     6241 agctgcttga cctgaaacaa actggcttgc tttgctatct aggcggaatt gaattggttc
     6301 cagtccagct tccttacgct ctgcaaccaa cacttgctcc aagcgaggaa ggaagcttgc
     6361 tgccttgttt tcatgaacca gcagaacctc catggcatta caaacagaag gacgactggt
     6421 tttagcattg ttgatgatag acagcgcctt gtcttcgtct gcatccttat ccacatagac
     6481 atggacaatc ccagtccctg tctcgataac aggtacaatc gcattctcaa ccactgcatt
     6541 gatcaagcca gctcctccac gaggaatgag aaggtctaga tagcccttgg ccttcatcat
     6601 agcataacta ctttcacggc tagtatcctc caccagttga atcacatttg gatgaatagt
     6661 agtcgtctcc aagcccttct tcaaggctgt gacaatggca tgggttgttt gataggcatc
     6721 cttaccacta cgaagaacaa ccgcatttcc actcttaaga gtcaaagcag ccgcatcaga
     6781 cgtcacattt ggacggcttt catagataat accgatgaca cccatagcta cacgtttttt
     6841 tgtgataacc aaaccatttt caagctgact tgtttctaaa acttcaccga ttggatctgg
     6901 taaggcaacc acttcacgaa ttcctcttgc catcgcttct atacgatctg catccaaata
     6961 aagacgatcc aacatcacat ctgagatttt ccccttagcc gctgccatat cgagggcatt
     7021 agccgctaaa atttcctcag tagcagccac taagtgatca gccatggcta gcaaggcttg
     7081 gtttttcact tcttcactag ctgtgttaat cgatttttta acagcctgta cctgttcaaa
     7141 ttgttcttgt ctactcacca tagtttacct ctaaaattct gtaaaaagta gttggatttc
     7201 aggagtaatg gaaatccagt cgtcacggta aatcaagaca cccttggctt tttgagaacg
     7261 caacatatcc tccaaagcag atgctccaaa ttgcacgcgt ccttttccaa gtgattttcc
     7321 actttccttg tcaaatactg tcacgatatc accgtaagaa aagactcctt ctgcttcaac
     7381 gataccagat aagagaagac tctttccata ttgagagaga gcttccgcag cccctttatc
     7441 aacccaaata gaaccttgac tctgagcata gaaggcaagc cattgtttct gggtacgaag
     7501 ccccttctct tgagcaacaa agtaagaacc atcctcggtc tcctctgccg cctcaatcat
     7561 ggaatctgat ttcaaggatg agcagatata aacaggaact cctgattccg tcgcgatagt
     7621 tgcagccttg attttggtta acataccccc agttccgttt gacgaaccag ctccaccagc
     7681 catatcaata atctcacgat tgatggtctc gattctctcc aagcgtttgg ctcttggatc
     7741 tgaattagga tttccagtat agagaccgtc cacatctgtc aagaaaacta aaaggtctgc
     7801 ttggaccatc gccgctactt gagcacttag agtgtcattg tccccaacct tgagctcatc
     7861 aataacgaca ctatcattct cattgatgat aggaattgcc ccacggttga gcaaaaccga
     7921 caaagcctga tgggcatttt tataacgacg cttatccaca aagtcatctt gggtcagcaa
     7981 gatttgtgca gaaacgattt gacgcaagag aagattggtt gtatattctt ccaacaaaag
     8041 cccctgccct accgctgctg aagcctgttt atcagcaatc ttagtcggac gctttttaaa
     8101 tcctaaggct ccaaaaccag ccgcaatggc acctgaagac accaaaatca actcatgacc
     8161 agcctcgtgc agcatagcca actgctgggt aatatccttt accttactac gtgataaact
     8221 tccatcctca tttgtcagag aagaagtacc caccttaaag acaatccgtt tgtatttcat
     8281 agtctcactc cgattattca tatttatcca ttataacatg aataacagga aatagaaaag
     8341 agttgatagg aaaaagaagg ccctaaaacc ttcttttcta ctactgttct gattccgatt
     8401 tgtttacact cgtttcagag ctttgtgaag ttgaggatgt caaatcttga ctagttgaaa
     8461 aatttgtaac actttcatgt tgactcgttt ctttattttc aagatccttt ttcactactt
     8521 ctttggattc ttttatttct gaatgacttg tagttttaat tgtagcagat ttctcgatcg
     8581 aaacatccac taaccaggca ccacttgaat ccacagtata accttctggt gtcttaccgt
     8641 tcacaagtaa ttgaccattt tctttcaaga agtaccattt atccttgtcc ttgatccaac
     8701 ccacagctcg tgaaccgtct gtcttgtaga aataccaatc atttgccttt ttatgccagc
     8761 cttgcttcat agctcctgaa tcttccagat aataatgttg accagcaact tctatctcac
     8821 ctgtcttcat gacaccagta gaatccatat agtaccaggt ttctttatct tttacccagc
     8881 ccattttcat ttcccctgat ggagcaaagt aataccattg ccccatatat tgaagccatc
     8941 ctgtcttcat agaaccatca acatccaaat aataccactg ctctttatct tgaatccaac
     9001 caatttccat ctggttttct ttgttaaaat aataccagat tccagcgatt ttcttccaat
     9061 ttttagcaga agcaccagag tctgtcaaat agaaccaatg attgttccat tttttccatt
     9121 gattctgtaa caagataccc gtttggttaa agtaatacca ttcaccttcg atttcattcc
     9181 aaccgacagc atactctcct gtagaatcag gcgcttgata ccaccagttc ccatatgcac
     9241 tcttatgcca accagcttga aaacttggaa tcggcttgta ggatgttgaa atattgacaa
     9301 aaccgtcttt tcgaatatca aaaactgttg catcatagtc tttgctggct gcgttgattc
     9361 tctcaattcc tcgttctttg agccaattaa catactcact atcaacacca tttttccaag
     9421 gtagactatc cgaagtttga acaatcaaac tcggactcaa atttttaatg aaatccttgg
     9481 tatttgattt gttggtatca tggtgatgat taaacttcat caaatcaact tttccaatga
     9541 gaggaccata cttgtcttct gctccatgaa cattatctaa atcgccccca aggtaaattt
     9601 tcttgccatt gactttcacc acgctaatca aggaattgga attgtcatcc caaattttct
     9661 ttaattcacc cgatgaatca gtttcatttt cataattata gagctgaata tccatgtccc
     9721 caaactgaaa atgagcatcc ccttgtgtga tattttgaat aactgaaaca cctttttctg
     9781 cagcagtctg taaaacctta tcatagccat acagattatc ccatagacgt tcagaattag
     9841 taatacgact atcactatat ttcttaagat agactcggtc aactggatag gtagacagta
     9901 attcatcaac atttccaata tgatcactgt gggtatgggt caccaaaata aaatcaagtt
     9961 tttggacacc caattccttc aaacgacgaa agacacggtc tgttagaaca tgcttataag
    10021 acgtttcaat tccttctctc catggatagc gagaatcact tccatctggg aaatcataat
    10081 cttctcctgt atccaccatg gcaaaatgtc cattgctttc aagaataatc gcatcactgc
    10141 caccttcttg aacattgata aagtggattt tatttcctga actttcttga gcctgaacat
    10201 tcccatacca tgacaaacct aaaaaagcgc ctacaagtgc taaactagtt aatttctttt
    10261 tcattcttac tttctcaatc tctccaaggt ttccttaaaa atctctggga tatctgcttt
    10321 aaattccaag gtcttacctg ttctcggatg agtaaaacct aaagtcttgg catgaagaaa
    10381 ttgtccatgt cctttcaaag tcttgcgagg accatagacc tcatcaccag cgactggatg
    10441 gccgatataa gccatgtgga cacggatttg atgagtgcgc cctgtctcca gttgcaactc
    10501 tactaagcta taatcgccaa agcgttccaa gacgtgaaaa cgcgtcactg caggcttccc
    10561 tttagcagtt acagcctgtt tcttacggtc tttttcactc cggccaatcg gcgcttcaat
    10621 tacaccacga tcattaggta gatttccatg aacaatcgcc caatatttgc ggagagactt
    10681 tttatccttg agttcttggg caagtgctag atgcgcatca tcgtttttag caatcatgag
    10741 aagacctgac gtatccttat caatacggtg aacaatccct ggacgcagaa ccccattgat
    10801 acccgacaag tccttaatat gatacatgag ggcatttact agggttccac tggtatgacc
    10861 agcactcggg tgcacaacca ttccctgagg tttgttaacg acagccacat cctcatcttg
    10921 gtagactatt tctagcggaa gatcctcagc cacatactct aatacctctg gttctggcac
    10981 atggtaagtg acgacatcac cctcttggac tgtgtattta gctttcttga cttgaccatt
    11041 gaccaagacc tggcctgatt taatttgttc attcgcgaga ctacgtgata attctgacaa
    11101 atctgacaaa gccttatcca aacgcagacc accagtttca attttaattt ccatttattt
    11161 cctcttttag cattgcaatc aataaaataa tcactccaac cgtcagatag ctatctgcca
    11221 cattgaaaat tgcaaagttg ataaagtcaa ggtggaacat atccacaaca aagccctgac
    11281 tgaccctgtc aataaagttt ccaagaccac ccgcgattat tagagtcaaa cccaagacca
    11341 tccagaatga gtcctccatg tgtttatgta aataccaaat ggcacctatc acgacaacca
    11401 gagtaatgac agcgaataac agctgctgat cttgtaagat agaaaaggct gcacctcgat
    11461 tttgcaggta ggtcaagcta acgaaattgg ggatccagga gcgcacttca cccagtggaa
    11521 tctgctggac gatataggat ttgaccaact gatccagccc aatcaaaagc agtacaatga
    11581 ctgccactat tgctcttttt ttcatgattt cctcttttga tcaaaatatt cttgcatgac
    11641 ttctacgaag agagtcccag cttgactaag ctccacttct tcacgtttaa catagaccat
    11701 gcggttatct aggttatcct tgagacgaat aactgtaatg ccattaacac tgtcactatc
    11761 taaaaatcca gaacctgtcg cataggcgtc cgtccgctcc aaaataccat tcaaggtggc
    11821 acggtctgtc acattaaaca tctgtgagct agcgctggta tcgacaaagt tctctgaata
    11881 ataaaggtac tcgtctttct cttgagtgaa acgaaccgtt ggtaaatccg ctaaatcctc
    11941 catgactaat tcctctttct gggctaaagg atgaccctca cggagataaa tatgggtatg
    12001 gaaaggaatc aattcgatga cctccagacc taatttttca acccgttgca taatcccctt
    12061 tttattttga ttgttgaggt agataatccc aatctcacta tgcccttgcg ccacttcatc
    12121 taatatttga acagtagttg attcaaaaat acggaagttc ttatagtcag gatagcgctc
    12181 tgaaaaggcc gtaatagttg gtggcaagaa gtcatagtgc tggctagcaa cagaaaattc
    12241 atctttttct tcttcaggat tggcatactg attttgaaaa atatcaaatc ctttaaccaa
    12301 ttcttgcgat ttttcataaa attccatccc acgacgggtc aagaaagtcc ctgagctggt
    12361 ccgacggaaa atcttaaagc ccaactcttt ttccaaatca cgaacagaaa tagacagact
    12421 cggctgacta acatacatct tttcagcagc ttcacgaaaa gtaccactat tggcaatagc
    12481 cacaacatag cgtaattgtt gaatgttcat cttctacccc caacttcttt atcttttcat
    12541 tataccatat tttagaagtt ttccaaaaag gaaaaaagaa catcctattc ttcttaacta
    12601 tcttcactat ctgcctttct caagtcaacc ttattttcaa aatcaattca aaaaaataac
    12661 tggatacaca ccactacaat atcgaaaaga aaaacgttga ctatttgtga aaaaagaaaa
    12721 tgccggaaag ttccgacatt attttagtaa gctaacttcc tgaaaatagg agcaaccaca
    12781 catcccagaa ggcagtgata ttaatgagaa tatgaactag tattggatag tagagatttt
    12841 tagtcatgcg atacaataga gctaaaataa gacctccact agcataaatg aaaaaagcaa
    12901 gaggagttaa agcaaaattg atatgaatat aaccgaaaat aatagcagaa agcaaaacat
    12961 ctccgtacca aggtgagttt ttgaaaaagg ttgtcataag cacacctcga taaatcaatt
    13021 cctcagcaat aggggcgatg aagcaaacga tgagcaagaa ataagggaac tcctgtctcc
    13081 ccatcatttc taccgtttca ttcaaagaaa tctgatttga agacagggat atgaaatacg
    13141 aaaagaggaa gtcagacata tatgaaatga tgtagcccag taaaaggtag atgaagtacc
    13201 tcagctgcca cttgaaatga aaaattttct ttcctgcaaa aaccagtaga tagatgaccg
    13261 ctaagaaaag aactccgctc tccatcaaaa gcagaatctg aaaaaattca cgacttgctg
    13321 gtagataggg ctttgcgaga taattgaaaa acctccaaaa ccaagttgat ttgtaaaaaa
    13381 ttaaggacaa tgctagtaaa atttgaatag ctcgtttttt catattaaat cctctgtttt
    13441 ctccccttgt ttcttcatat tcctaataaa ttttacttaa aatctcgcag cacttccttt
    13501 gcaaagatga ttacctcctc caatatatcc tgaatcatag gtgcctctgg gacaaaatcc
    13561 atgaaccagc agagttaata ctggttctat ttcccaatcg tacctaccat taggattttt
    13621 accatatgga tctgctccac cttgtatcag ctctaattct tcatttgtta gatttatatt
    13681 ttttgtcatt aaaatcatga tatatttcct ccattattct tcagattagt tgagtaatct
    13741 gctactcata cctacttaca aaaaaactat tatattaagt tggtttttta atttgtctag
    13801 ttgcaacatt gacaaacata gtatagcata tctttgcgaa atatcctctt caaatcatga
    13861 attgtcatca aaacatctta aactataaaa tcaattagtc tcaagctttc tatcaatttc
    13921 ttctccaaaa tatgctataa taatagcaaa agataaagaa ggaagaccta tgattaaact
    13981 actagccttg gatatggacg gcaccctcct caatgaagcc aaggaaatcc cacaagctca
    14041 cattactgct attcacaaag ccattgaaaa aggtgtcaaa ctggttctct gtacgggtcg
    14101 cccccttttc ggtgtcctcc cctactacaa aaaactggag cttgacctcc agaacgaata
    14161 tattattgtt aacaacggtt gttcaactca ccagaccagt gattggagtc tagttgactg
    14221 gaaagaactg agtccagctg acatcgaata cctctatgac cttgctgaaa agagtgatgt
    14281 tcagttgaca ctttttgacg agtcacatta ttttgttctc ggtgacaagc ccaatcaagt
    14341 tattgaaaat gatgctaaac tagtattttc agacctgact gaaatttctc ttgaagaagc
    14401 gactagtgga aagttccgga tgttccaagg tatgttttta ggaacaaaag aacaaacaga
    14461 cgattttgag cagcgttttg ctgaagagct ttgccaacga ttcagtggag ttcgttcgca
    14521 gcctgtcatt tatgaagcaa tgccgcttgg aacgacaaag gctactgctc tttcacgact
    14581 agctgagatt ttgaagattg attcctcaga gattatggcc atgggcgatg ctaataacga
    14641 tatcgaaatg ctccagtttg cagggcttgg gattgcaatg ggaaatgcca gcgattatat
    14701 caaatctctt gcggatgccg ttacctcaag caacgaagaa gacggcgttg cgcgtgctat
    14761 tgagaaatat attctataaa aaagaaaagc aaatagacag aagttactgc tatttgcttt
    14821 tttgctaata ttttaaaata cggactaatc aatagacaag aatagcgaat cataaaaatc
    14881 gatttactag atgccatagt atcttatagt tactaataag aagttagtct agcagaatga
    14941 atctctcatt tctcccacac taatctgtaa tttgtctata catttctggt cttctatctc
    15001 gaaacaagcc ccagtttagg cgttcacttg ctcccttgtc taggtcataa gtagctaaca
    15061 gaacagcttc ttcttgtctt tcagctcgtt ctagaatagc tcctgtttca tccgtcataa
    15121 aggaggaacc gtagaagtca agactggagc tctgtccgcc attttcctca ctaggagtaa
    15181 cctcctctaa accataacga ttggctgcga tgactggaac aatattcgct gctgcgtgcc
    15241 cttgcatagt acgttgccag tgaccacaac tatctgtatc caaaattggc tctgaaccga
    15301 tagctgtagg ataaaagagc aattcagcac cattcaatgc aagacagcgc gctgtttcag
    15361 ggaaccattg atcccaacag ataccgatac caatcttagc atagcgagta ttccagacct
    15421 tgaaaccagt gttaccaggc gtgaaataga atttttcttg ataataatgg tcatctggta
    15481 tatgggtctt tcgataaacg cccagcactt ccccatctgc atcaatgacg gcaatagagt
    15541 tatacaagac attaccatct ttttcataga aactgattgg taaaacaact tgtagttcct
    15601 tagcaatcac cttaaaatgc tgaatggcag tattttccgc tacagattgg gcatactggt
    15661 agtagtcata ctgacgttcc tgacagaaat agggatgttc aaacaactcg ggcaagagaa
    15721 taatttgggc tccttgctca gcagcctgac gtactaaacg ctctgcggtt tggatatttg
    15781 ttgccacatc cttagcgcat tgcatctgaa tggttgcaac tcttacattt ctcatctttt
    15841 tctcctattc tggaatttgt tgggtgatac agtggatatt gccaccacct aagagaatat
    15901 ctctggctgg tattccgaca actttacggt ctgggaaaca cttgctgagg atatctaagg
    15961 ccacttggtc gtttacatcc tcaaactgtg gaaccaagac agccttgttg gcgatataaa
    16021 agtttacgta ggaagctgct agtcgttcac ctgcgtatcg cttttcttct ccttcttcat
    16081 agatgtagcc tggcaaatct tcttctgtca caacttgtcg aactgcaggg ataggcaatt
    16141 tatgaatggt gaagtgacaa ccttttgcat ctgtttcctg ttctaagagt tcgagatctg
    16201 cttttgacat ggcatactgg ggatcatttt cgtcatctgt ccaagccaaa acaagctcag
    16261 caggaccaac aaaggcagca acattatcga cgtgttcatt ggtttcatcc tgataaatac
    16321 cataaggaag ccaaataact ttttcagcac caagactttc taataatgtg ttttcaatct
    16381 cctctttagt caagttagga ttgcgaccag gactaagcaa gcaactttca gttacgagaa
    16441 tagttccttg accatcgcta tggattgcgc ctccttccag tacaaaaggt ttagcatcat
    16501 agacaggcct ttccaaggcc tcagcaaaac gactggctac ttggtcatcc tcttcataat
    16561 cttgataaag accatcatag gtgcctcccc aagcattgaa ggcccaatcc acggctaatt
    16621 tcttaccttt atcattgacg agaatggttg ggccagtatc acgcgcccag gcatcattgg
    16681 tgggaatgtc taaataaaca accttgtctc caagatagga ttgggcttca gatagatagg
    16741 cctgctccac caaaagatag actctttccc cttctgctat ggtctcgata atctgagtaa
    16801 atgctctttt agcagccttt ccttgaaaag gccatgatcc tggtcgagtc ggccatatca
    16861 tgagggtacc atgatggggt tcgtactctg ctggcatgtg atagcctaat ttttttggac
    16921 tgtccatcat gataatctcc ctttaaagtc ttgatagcca aaagctttga gtaagctaca
    16981 gtctccctgt tcgtccatga gatagagact tggcaatcca ataccattaa aggtattatt
    17041 tttgacaaaa gaataaatgg ccatgtcttg aaaataaagt ctgtctccga tttggactgg
    17101 attttcaaaa ctataatcac caatcacatc gcccgtcaga caggtattag aagaaagtct
    17161 gtaggtatgg gctttttcct gtgactcaaa gccatttctc aaaggtggac gatagggcat
    17221 ctcaagtaca tcaggcatat ggcaggtcgc agaggcgtct aaaaccaaga tttccatacc
    17281 gttttctaca atatctaata cctcagttgc taaataaccc gcattaagcg caatggcttc
    17341 accaggctcg atatagattt caagattgta agtttttcgg atacgcttga tttctgaaat
    17401 cagcaaatcc acatcgtaac cttctcttgt aatatgatga ccaccaccca tattgagcca
    17461 ttttacctca tgtaagtagg gaccaaactg ttcttctact gctttcaaag ttgtttgtaa
    17521 atcatctgct ccctgctcgc aaagggtatg aaaatgaagt ccgtcaacca aatctagcaa
    17581 atcactcgga atcttgtcta tagtaactcc aaagcgagaa cctggtgcac aagggtcata
    17641 gagcgcgtga tctccttgag ttgaacactg agggttgagg cgcaaaccaa cactgacacc
    17701 agcctctcga caacgcggac cgtgtttacg caactgtctc tctgagttaa agactatatg
    17761 gtccattatc tctagcaatt cctccaagtc tgcatccttg aaagcaggcg caaatacatg
    17821 gacttcacca ggaaattctt cccttgccaa tttggcctca tagagtccac tagctgtcgt
    17881 acctgataga tactggctaa tcaagggata agttttgtag agggaatatg ccttctgggc
    17941 aagcaagacc ttgcaaccgg cctcttcttg tacatattgt agaatgcggc aattagcttc
    18001 taacttggcc aagtcaataa cataggctgg tgttggtact tgttctaact tcattagtcc
    18061 accatttgtg gattttcaac cacaacccat ggcaaaccat actcattcaa agcttccatg
    18121 aatggatctg gatctaactc ctcaaggtta tacactccag cttgtttcca agttccgttc
    18181 atgactaatt ttgtcccaat catggctgga actcctgtcg tataagaaat agcttgcgaa
    18241 ccaacctctg cgtaacattc ctgatggtcg cagacattgt agatatagat agtctttttg
    18301 acaccgtctt tgacacctgt aaagatacat ccaatattgg tttttccgac tgtacgtggc
    18361 ccaagactgg caggatctgg aagcaaggct ttcaaaaatt gaattggaac aatttcttgg
    18421 ccgttaaagt taatggtatc cgtacgaagg agtccaacat tttcaagaca tttcatgtgc
    18481 gtcaagtaag attgaccaaa agtcataaag aaacgaatgc gtttgacacc tggaatgttc
    18541 ttggccaatg attcgatttc ttcatggtga aggagataca tatctttttg tccaacttga
    18601 gggaaatcat actcacgctt gatagacata gcttcgactt cgacccattt cccatcttcc
    18661 cagtaagaac ctggcgcaga aacctcacgg agattaattt ctggattaaa gttggttgca
    18721 aatggataac cgtggtcacc gccattacag tctaaaatgt cgatataatg gatttcatca
    18781 aaatagtgtt tgagggcata agctgaaaag acactagtta cacctgggtc aaaaccagaa
    18841 ccaagaagag cagtcaagcc tgcttctttg aatttctctt gataagccca ctgccatgag
    18901 tagtcaaagt aggctgtaaa accaagttcc ttacaacgtt tttcgtagat agcacgccac
    18961 tcagggtctt ctgtgtcttc tgcttcgtag ttggctgtat cgatatagtg aacacctgtt
    19021 gccaaacaag catccataat ggttaaatct tgataaggca gagctacatt caaaacagct
    19081 tctggtttgt agctttcaat cagggcaatc acttcttcaa ccttgtcagc atcaagtgct
    19141 gcagtttcaa ttttagtact tgttttgcct tctagcttcg ctttcaagtc atcgcatttt
    19201 gacttggtac ggctagcaat cataatctct gtaaatgttt cgctatcttg acaaatcttt
    19261 gaaatagcaa cttgggcaac gcccccacaa ccaataacta gtaaacgact catttttttc
    19321 cttcctcttc ttctaaaatg tcctcaacat acttgggcaa cataaaggct cccacgtgta
    19381 agtttgcagt gtagtattct gtgaaaagct ggcgtttttt ccagccttcc ttgtcaaaat
    19441 ctttgacagg gtggtatttt ttcgatgcaa atccaaacaa ccaatagcca gctgggctag
    19501 ttggaatatg ggcctgataa acccgactga ttggaaaggc ttgattgacc ttgcggtgca
    19561 tgcttcggca ggccgactca tcctcgtcaa agaagggact cccatgctgg taaatcatga
    19621 tgccgtcttc cttcagagct cgataactat tgccgtagaa ttccttggta aagagtcctt
    19681 ccgtatggcc aaatggatct gtcgcatcgt tgatgataat atcgtaatca tcttcgcagt
    19741 ttcgcaaaaa gcgtagccca ttttggtagt aaatggtaac acgaggatca tctagccctg
    19801 cagcaaagtc tgggaaatac tcacgacaga cctcgaccaa catctcatcc ggttccacaa
    19861 tatcaatttg ctccagttca ggatagaggg ttaatacttg ggcaacaccg ccgtcaccac
    19921 ccccaataac caatactttc tttggatttg ggtggacagc catgggaacg tgaacggtca
    19981 tttcattgta gacgaaatca tccgcatctg agaacaagac atggccattt aaaatcagta
    20041 ttttcccaaa agctggcgta tccaagactt cgatatcctg ccattcactt tttccagcgt
    20101 aaagttgctt ggctgttctc agagacaatt tgacatctgg agtatgaact tcagaaaacc
    20161 ataaatccat ctatttctcc ttatattata tttagcgcag ttagctactg ccaaagccca
    20221 agtggtatac ttggaataag ccactgtgga ttagttcatt tcctccaatc gcttgacgat
    20281 ttccgaaagg ctctaaccac agttttttgt ttcaattggt aatgagtttg ataacgcata
    20341 gacgttttat ctcgtctaag gtaacaaggc aaaaagttcg tggataacc
//
LOCUS       DAVKNL010000036        19532 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVKNL010000036 DAVKNL010000000
VERSION     DAVKNL010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19532
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.036"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(131..355)
                     /locus_tag="VW411_001325"
     CDS             complement(131..355)
                     /locus_tag="VW411_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory protein Asp5"
                     /protein_id="HEU9794047.1"
                     /translation="MIEILIVLAIILSLALIVLVTIQPRQNQLFSMDATSNIGKPSYW
                     QSNTLVKVLTLLVSLALFILLLTFMVITYK"
     gene            complement(360..539)
                     /gene="asp4"
                     /locus_tag="VW411_001326"
     CDS             complement(360..539)
                     /gene="asp4"
                     /locus_tag="VW411_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory system protein Asp4"
                     /protein_id="HEU9794048.1"
                     /translation="MSEEDLFYKDVEGRMEELKQKPIKKEKETRGEKISKTFSLLLGL
                     MILIGLLFTLLGILR"
     gene            complement(541..1878)
                     /gene="gtfB"
                     /locus_tag="VW411_001327"
     CDS             complement(541..1878)
                     /gene="gtfB"
                     /locus_tag="VW411_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HEU9794049.1"
                     /translation="MIELYDSYSQESRDLHESLGATGLSQLGVVIDADGFLPDGLLSP
                     FTYYLGYEDGKPLYFNQVPVSDFWEILGDNQSACIEDVTQERAVIHYADGMQARLVKQ
                     VDWKDLEGRVRQVDHYNRFGACFATTTYSADSEPIMTVYQDVNGQQVLLENHVTGDIL
                     LTLPGQSMRYFANKVEFITFFLQDLEIDTSQLIFNTLATPFLVSFHHPDKSGSDVLVW
                     QEPLYDAIPGNMQLILESDNVRTKKIIIPNKATYERALELTDEKYHDQFVHLGYHYQF
                     KRDNFLRRDALILTNSDQIEQVEAIAGALPDVTFRIAAVTEMSSKLLDMLCYPNVALY
                     QNASPQKIQELYQLSDIYLDINHSNELLQAVRQAFEHNLLILGFNQTVHNRLYIAPDH
                     LFESSEVAALVETIKLALSDVDQMRQALGKQGQHANYVDLVRYQETMQTVLGG"
     gene            complement(1871..3382)
                     /gene="gtfA"
                     /locus_tag="VW411_001328"
     CDS             complement(1871..3382)
                     /gene="gtfA"
                     /locus_tag="VW411_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HEU9794050.1"
                     /translation="MTIYNINLGIGWASSGVEYAQAYRAGVFRKLNLSSKFIFTDMIL
                     ADNIQHLTANIGFDDNQVIWLYNHFTDIKIAPTSVTVDDVLAYFGGEESHREKNGKVL
                     RVFFFDQDKFVTCYLVDENKDLVQHAEYVFKGNLIRKDYFSYTRYCSEYFAPKDNVAV
                     LYQRTFYNEDGTPVYDILMNQGKEEVYHFKDKIFYGKQAFVRAFMKSLNLNKSDLVIL
                     DRETGIGQVVFEEAQTAHLAVVVHAEHYSENATNEDYILWNNYYDYQFTNADKVDFFI
                     VSTDRQNEVLQEQFAKYTQHQPKIVTIPVGSIDSLTDSSQGRKPFSLITASRLAKEKH
                     IDWLVKAVIEAHKELPELTFDIYGSGGEDSLLREIIANHQAEDYIQLKGHAELSQIYS
                     QYEVYLTASTSEGFGLTLMEAIGSGLPLIGFDVPYGNQTFIEDGQNGYLIPSSSDHVE
                     DQIKQAYAAKICQLYQENRLEAMRAYSYQIAEGFLTKEILEKWKKTVEEVLHD"
     gene            complement(3394..5766)
                     /gene="secA2"
                     /locus_tag="VW411_001329"
     CDS             complement(3394..5766)
                     /gene="secA2"
                     /locus_tag="VW411_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HEU9794051.1"
                     /translation="MFRRLGQDFQLRKVKKILKQINALKGKMSSLSDQELVAKTVEFR
                     QRLSEGESLDDILVEAFAVVREADKRILGMFPYDVQVMGAIVMHYGNVAEMNTGEGKT
                     LTATMPVYLNAFSGEGVMVVTPNEYLSKRDAEEMGQVYRFLGLTIGVPFTEDPKKEMK
                     AEEKKLIYASDIIYTTNSNLGFDYLNDNLASNEEGKFLRPFNYVIIDEIDDILLDSAQ
                     TPLIIAGSPRVQSNYYAIIDTLVTTLVEGEDYIFKEEKEEVWLTTKGAKSAENFLGID
                     NLYKEEHASFARHLVYAIRAHKLFTKDKDYIIRGNEMVLVDKGTGRLMEMTKLQGGLH
                     QAIEAKEHVKLSPETRAMASITYQSLFKMFNKISGMTGTGKVAEKEFIETYNMSVVRI
                     PTNRPRQRIDYPDNLYITLPEKVYASLEYIKQYHAKGNPLLVFVGSVEMSQLYSSLLF
                     REGIAHNVLNANNAAREAQIISESGQMGAVTVATSMAGRGTDIKLGKGVAELGGLIVI
                     GTERMESQRIDLQIRGRSGRQGDPGMSKFFVSLEDDVIKKFGPSWVHKKYKDYQVQDM
                     TQPEVLKGRKYRKLVEKAQHASDSAGRSARRQTLEYAESMNIQRDIVYKERNRLIDGS
                     RDLEDVVVDIIERYTEEVAADHYASRELLFHFIVTNISFHVKEVPDYIDVTDKTAVRS
                     FMKQVIDKELSEKKELLNQHDLYEQFLRLSLLKAIDDNWVEQVDYLQQLSMAIGGQSA
                     SQKNPIVEYYQEAYAGFEAMKEQIHADMVRNLLMGLVEVTPKGEIVTHFP"
     gene            complement(5759..6199)
                     /gene="asp3"
                     /locus_tag="VW411_001330"
     CDS             complement(5759..6199)
                     /gene="asp3"
                     /locus_tag="VW411_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HEU9794052.1"
                     /translation="MIITQRQSIHWGEVGGTYMYGTTVSYYPDKSVRLYNPLLPSGEI
                     LKTWFSSVNYQAARTQPQLPLLKRKQEYQLSLVFDCQPENGVYTKITFFDRYGDILEK
                     KVEKVKDFIFTYPEDSYTYRVSLLSAGFESLTFYHFSIKEIRSV"
     gene            complement(6196..7731)
                     /gene="asp2"
                     /locus_tag="VW411_001331"
     CDS             complement(6196..7731)
                     /gene="asp2"
                     /locus_tag="VW411_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HEU9794053.1"
                     /translation="MSKELNILQIGLANWENHYDIPENMSWYYFYPNSSKALREIIEK
                     EDINRFHAVLIEDGQYSRDLFSYVKYFEPYTLFYNQNLQINDREVVDFLKKRCAQAID
                     FLSPQQLINDLSKSLFGGGYGDKLFPPTIQVNPNFTGAISYQGLDYVSLEGEFGQDFA
                     QLAYWAYNIMVQKTLPIELWLEYEKEGNCDFRLVIRKMWSGSVDDFFEEVIVSEKDLE
                     QALFMDSRDGDYFLSISVEARGRGTIKLGNLHQRWSRKQFGKFVLGGNILHDSKRDEI
                     NYFFHPGDFKPPLTVYFAGYRPAEGFEGYFMMKTLGCPFILFSDPRLEGGAFYLGTDE
                     LEGKVKDTITHYLDYLGFDHKDLILSGLSMGTFPALYYGASFEPHAIIVGKPLANLGT
                     IASRGRLDAPGVSNLAFDCLIHHTGGTSSQDMTELDQRFWKIFKQANFSKTTFGLSYM
                     KDEEMDPQAYEQLVSYLCNTGAKILSKGTAGRHNDDTDTNISWFLHFYRMVLETGFGR
                     EKR"
     gene            complement(7715..9295)
                     /gene="asp1"
                     /locus_tag="VW411_001332"
     CDS             complement(7715..9295)
                     /gene="asp1"
                     /locus_tag="VW411_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HEU9794054.1"
                     /translation="MYYFIPAWYGSERTWHADITPWYFSHFRLEFDDTFHQIRLFQEQ
                     DIDSRLLVLAYQPHLRYFLYRHGVLEMDTYSVFDVMQDFHNLHTQVLSIRDIEWDDDC
                     EFIYSPFTIIVQKNGKKFAKVEHGVEGFISDIQYFEPNGQIHMHHIVDDRGFVSSIIF
                     FEDGQAAYQEYLNLKGEWQFRERLKEGGQVEVNPILGYRFKMLTYQNMGDLVAEFFEN
                     YLQTYVKDQDIFMLPSHSHHDQLVLDRLPSTNPKLLSLFIGRNPQDTFRDLDVTFEKS
                     DLILVDREDSLRLLQELYPERMHQCYHLSSFDTRLRLGRSQTKKESIIYFQLDFEQGI
                     DNQALLQVLSFVAENKDTEVIFGAFAASQEQMNEVEGIVESFIQENIQSENLGKAIDY
                     GDAENPLEENQHQDLRLQFVNLNDELDLIKTLEFVRLIVDLNRHPHLYTQIAGISAGI
                     PQINLVETVYVEHLKNGYLLADVTEFSKAAHYYTDRLKEWNESLIYSIDKIKEHTGQQ
                     FLGKLEKWIEEVKNVKGT"
     gene            complement(9305..10522)
                     /gene="secY2"
                     /locus_tag="VW411_001333"
     CDS             complement(9305..10522)
                     /gene="secY2"
                     /locus_tag="VW411_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HEU9794055.1"
                     /translation="MTKIYSSIAVKKGLFTSFLLFIYVLGSRIILPFVDLNTKDFLGG
                     STAYLAFSAALTGGNLRSLSIFSVGLSPWMSAMILWQMFSFSKRLGLTSTSIEIQDRR
                     KMYLTLLIAVIQSLAVSLRLPVQSSYSAILVVLMNTILLIAGTFFLVWLSDLNASMGI
                     GGSIVILLSSMVLNIPQDVLETFQTVHIPTGIIVLLALLTLVFSYLLALMYRARYLVP
                     VNKIGLHNRFKRYSYLEIMLNPAGGMPYMYVMSFLSVPAYLFILLGFIFPNHSGLAAL
                     SKEFMVGKPLWVYVYISVLFLFSIIFAFVTMNGEEIADRMKKSGEYIYGIYPGADTSR
                     FINRLVLRFSVIGGLFNVIMAGGPMLFVLFDEKLLRLAMIPGLFMMFGGMIFTIRDEV
                     KALRLNETYRPLI"
     gene            complement(10579..11256)
                     /locus_tag="VW411_001334"
     CDS             complement(10579..11256)
                     /locus_tag="VW411_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9794056.1"
                     /translation="MGISVAKGEYITIVDSDDIVKENMIETLYQQVQEKDADVVIGNY
                     YNYDESDGNFYFYVTGQDFCVEELAIQEIMNRQAGDWKFNSSAFILPTFKLIKKELFN
                     EVHFSNGRRFDDEATMHRFYLLASKIVFINDNLYLYRRRSGSIMRTEFDLSWARDIVE
                     VFSKKISDCVLAGLDVSVLRIRFVNLLKDYKQTLEYHQLTDTEEYKDICFRLKLFFDA
                     EQRNGKS"
     gene            complement(11241..11483)
                     /locus_tag="VW411_001335"
     CDS             complement(11241..11483)
                     /locus_tag="VW411_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9794057.1"
                     /translation="MSELISVVVPIYNTGKYLVECVEHILKQTYQNIEIILVDDGSTD
                     NSGEICDAFMMQDNRVRVLHQENKGGQHKLKIWGLV"
     gene            complement(11483..12679)
                     /locus_tag="VW411_001336"
     CDS             complement(11483..12679)
                     /locus_tag="VW411_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEU9794058.1"
                     /translation="MRKSIVLAADNAYLIPLETTIKSVLYHNRDVDFYILNSDIAPEW
                     FKLLGRKMEVVNSTIRSVHIDKELFESYKTGPHINYASYFRFFATEVVESDRVLYLDS
                     DIIVTGELATLFEIDLKGYSIGAVDDVYAYEGRKSGFNTGMLLMDVAKWKEHSIVNSL
                     LELAAEQNQVVHLGDQSILNIYFEDNWLALDKTYNYMVGIDIYHLAQECERLDDNPPT
                     IVHYASHDKPWNTYSISRLRELWWVYRDLDWSEIAFQRSDLNYFERSNQSKKQVMLVT
                     WSADIKHLEYLVQRLPDWHFHLAAPCDCSEELTSLSQYTNVTVYQNVLHSRIDWLLDD
                     SIVYLDINTGGEVFNVVTRAQESGKKIFAFDITRKSMDDGLYDGIFSVERPDDLVDRM
                     KNIEIE"
     gene            complement(12688..13908)
                     /locus_tag="VW411_001337"
     CDS             complement(12688..13908)
                     /locus_tag="VW411_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HEU9794059.1"
                     /translation="MRNTKRAVVFAGDYAYIRQIETAMKSLCRHNSHLKIYLLNQDIP
                     QEWFSQIRIYLQEMGGDLIDCKLIGSQFQMNWSNKLPHINHMTFARYFIPDFVTEDKV
                     LYLDSDLIVTGDLTDLFELDLGENYLAAARSCFGAGVGFNAGVLLINNKKWGSETIRQ
                     KLIDLTEKEHENVEEGDQSILNMLFKDQYSSLEDQYNFQIGYDYGAATFKHQFIFDIP
                     LEPLPLILHYISQDKPWNQFSVGRLREVWWEYSLMDWSVILNEWFSKSVKYPSKSQIF
                     KLQCVNLTNSWCVEKIDYLAEQLPEVHFHIVAYTNMANELLALTRFPNVTVYPNSLPM
                     LLEQIVIASDLYLDLNHDRKLEDAYEFVLKYKKPMIAFDNTCSENLSEISYEGIYPSS
                     IPKKMVAAIRSYMR"
     gene            complement(13939..16383)
                     /locus_tag="VW411_001338"
     CDS             complement(13939..16383)
                     /locus_tag="VW411_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
                     /protein_id="HEU9794060.1"
                     /translation="MNKTIVLAGDRNYTRQLETTIKSILYHNRDVKIYILNQDIMPDW
                     FRKPRKIARMLGSEIIDVKLPEQTVFQDWEKQDHISSITYARYFIADYIQEDKVLYLD
                     SDLIVNTSLEKLFSICLEEKSLAAVKDTDGITFNAGVLLINNKKWRQEKLKERLIEQS
                     IVTMKEVEEGRFEHFNGDQTIFNQVLQDDWLELGRAYNLQVGHDIVALYNNWQEHLAF
                     NDKPVVIHFTTYRKPWTTLTANRYRDLWWEFHDLEWSQILQHHMGEFELISPLDKEFS
                     CLTLTNSQDLEGIEELVTALPEVVFHIAAWTDMGDKLKKLAVYNNVRLHPQIVPPVLD
                     KLKKSTNLYLDINHGSADENFLKSLQEQEKTLLAFQSTQHGELGQIVFENGKVSFMID
                     TIKDFKKNGHLTCFRQLPSLTCLTFTASQYIEQLDYLAGQLPNVVFQIAAWTAMGPKL
                     YDLSNRYPNIQLYPAISRDKLDELKEKMDAYLDINLLTSTSDIVAEMAHLSKPILAFY
                     KSQNGNNGQRLYSSEHPERMLADLQKLITKDMLEKPLDIIQVKGIDETLDYIIEHNSS
                     LVRFGDGEINMLAGHSIPYQDYDEELVSIMRDIIGQESREDLVVCLPDAFTDRFRFTS
                     WAIPFWKDHMDHYMDFYRELCSDSWYGSTFVSRPYIDFEDKSQAKAQFEKLKSIWENR
                     DLLIVEGATSRSGVGNDLFDEANSIKRIICPSHSAFSRVHELEQEIEKYAGGRLILCM
                     LGPTAKVLSYNLCQMGYQVLDVGHIDSEYEWMKMGAKTKVKFSHKHTAEHNFDQDIEF
                     IDDETYNSQIVARILN"
     gene            complement(16393..17403)
                     /locus_tag="VW411_001339"
     CDS             complement(16393..17403)
                     /locus_tag="VW411_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU9794061.1"
                     /translation="MKLHLTNLYGMAGDSTVILAQNAVQKIASQLGFREVGIYFYNIA
                     SDSPSEMNKRLDGIMASISIGDILVFQSPTWNGFEFDRLLFDKLKDMQVKIICFIHDV
                     VPLMFDSNYYLMKDYLYMYNLSDVLIVPSERMKTRLMEEGLTTKKILVQGMWDHPHDL
                     SLYTPAFKKELFFAGSLERFPDLQNWSQDTPLRVFSNKGEASSSARSLSIEGWKKDEE
                     LLLELSKGGFGLVWGTHQNEGESNQYYTLNISHKVSTYLTAGIPVIVPSSLSTAKFIV
                     DQGLGFMADSLEEVHEIVDKMNLQEYQEMTNRIKTFSYLLKEGYFTKKLLVDAIYHLG
                     ID"
     gene            complement(17432..18022)
                     /locus_tag="VW411_001340"
     CDS             complement(17432..18022)
                     /locus_tag="VW411_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEU9794062.1"
                     /translation="MEHDALDRLYGALKKENADISIGRYNSYDETRYVYMTYVTDPDD
                     SLEVIEGKAIMDREGVEEVRNGNWTVAVLKLFKRELLQDLPFPIGKIAEDTYWTWKVL
                     LRASRIVYLNRCVYWYRVGLSDTLSNTWSEKRMYDEIGAREEKIAILASSDYDLTNHI
                     LIYKNRLQRVIAKLEEQNMQFTEIYRRMMEKLSLLP"
     gene            complement(18030..18308)
                     /locus_tag="VW411_001341"
     CDS             complement(18030..18308)
                     /locus_tag="VW411_001341"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9794063.1"
                     /translation="MADELISIVVPIYNVENYLRMCLDSIQNQTYQNFECLLINDGSP
                     DHSSKICEEFVEKDSRFKYFEKANGGLSSARNLGIECSGGGVHYFCRL"
     gene            complement(18313..19527)
                     /locus_tag="VW411_001342"
     CDS             complement(18313..19527)
                     /locus_tag="VW411_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEU9794064.1"
                     /translation="MNTKSIVFNADNDYVDKLETAIKSICCYNNCLKFYVFNDDIASE
                     WFLMMNKRLKTIQSEIVNVKIVDHVLKKFHLPLKNLSYATFFRYFIPNFVKESRALYL
                     DSDIIVTGSLDYLFDIELDGYALAAVEDSFGDVPSTNFNSGMLLVNVDTWRDEDACSK
                     LLELTNQYHETAYGDQGILNMLFHDRWKRLDRNFNFMVGMDSVAHIEGNHKWYEISEL
                     KNGDLPSVIHYTGVKPWEIISNNRFREVWWFYNLLEWSDILLRKDIISRSFEELVYSP
                     KAHTAIFTASCEMEHVEYLIENLPEVHFSILAHTYFASSVVALLRYSNVTIYPCFSPF
                     DYRKILDNLDFYLDINHYKEVDNIVSVVQQLSKPIFTFENTSHDIGNQTNIFSSTEPN
                     KMVEAIRQFIGE"
BASE COUNT         6343 a         4152 c         2968 g         6069 t
ORIGIN      
        1 tatctacaac ctcaaaacgg tgttttgagc agcctgcggc tagcttccta gtttgctctt
       61 tgattttcat tgagtataaa aaagaaaact tcagaccaat ccacaaaagg tgaatatctg
      121 aagttttctt ttatttataa gtaatcacca taaaggttaa tagtagaata aataaagcca
      181 aactcaccaa taaagtgagc accttgacca aggtgttgct ctgccagtag cttggtttac
      241 caatattact agtggcatcc atggaaaata gttgattttg acggggttgt atagttacca
      301 atacaatcaa agcaagagat aggataatag ctaaaacaat tagtatttca atcatagatc
      361 tacctcaaaa ttcccagcaa agtaaagagc aaaccaatca gaatcatcaa acccagtaaa
      421 agtgaaaaag tcttactaat cttttcccct cgggtttctt tttccttctt gatgggtttt
      481 tgtttcaact cttccatgcg gccttcaacg tctttgtaaa ataaatcttc ctctgacatg
      541 ttagcctcct aaaacagttt gcatggtttc ctgatatctc accaagtcaa cataatttgc
      601 atgttggcct tgtttgccaa gtgcctgacg catttgatca acatctgaaa gggccaattt
      661 aatggtctca accaaagcag caacttcact actttcaaat agatggtctg gagcgatata
      721 aagtctattg tgcaccgtct gattaaagcc aagaatcaag agattgtgct caaaggcctg
      781 acgcactgcc tgtagcaact cattactgtg gtttatatcc aagtaaatat ccgacagttg
      841 atacagctcc tgaatcttct gtggactagc gttctggtaa agggccacat taggatagca
      901 aagcatgtct aagagcttag aagacatctc tgtcaccgct gcaatacgga aagtgacatc
      961 aggcaaggct cctgcgattg cttctacttg ctcaatctga tctgaattgg tcaagattaa
     1021 ggcatctcgt cttaggaaat tatcacgttt gaactggtaa tgataaccca agtgcacaaa
     1081 ctgatcatgg tatttctcgt cagttaactc taaagcgcgc tcataagtcg ccttatttgg
     1141 aatgatgatc ttcttagtac gcacattatc actttccaaa atcaactgca tattacctgg
     1201 aatggcatca tagagaggtt cctgccatac caagacatcc gagccagatt tatctggatg
     1261 atggaaggaa accaagaaag gagtcgctag agtattaaag ataagctgac tggtatctat
     1321 ttccaaatct tgcaaaaaga aggtgataaa ttcaacttta tttgcaaagt aacgcatgga
     1381 ctgacctggc aaagtcaata agatatcacc cgtcacatgg ttttccagta aaacttgttg
     1441 accattgaca tcttggtaaa ctgtcataat cggctcgcta tctgcgctat aagtcgttgt
     1501 agcaaaacaa gctccgaagc gattgtagtg gtcaacctga cgtactcgac cttctaggtc
     1561 tttccagtct acctgtttaa ccaagcgagc ctgcattcca tcagcataat gaatgacagc
     1621 cctctcctgc gtcacatctt caatacaagc agactgatta tctcctaaaa tttcccaaaa
     1681 atctgaaacg ggaacttgat taaaatagag aggttttcca tcctcgtaac ctagataata
     1741 ggtaaaagga gaaagcagac catcaggcag aaaaccatct gcatcgatga ccactccaag
     1801 ttgagaaaga ccagtagcgc ctagactttc atgtaaatct cgactttctt gactgtaact
     1861 atcataaagt tcaatcatgg agcacctcct ctactgtttt cttccacttt tctaaaattt
     1921 ctttggtcaa gaagccttct gcaatttggt aagaataggc acgcatagct tccaaacgat
     1981 tttcttgata caattgacaa atcttagcgg cataagcttg cttgatttgg tcttctacat
     2041 ggtcagatga acttggaatc aaataaccat tttgcccatc ctctataaag gtctgattac
     2101 cataaggcac atcaaaacca attagaggta gacctgaacc aatagcttcc atcaaggtca
     2161 gaccaaatcc ttcgctggta gaagccgtta agtagacctc atactggcta taaatctgcg
     2221 aaagttccgc atgccccttg agttggatat agtcctctgc ctgatgattt gcaataattt
     2281 ctctaagcag agaatcttct ccaccactac catagatatc aaaggttagt tccggtaact
     2341 ccttatgagc ttcaatcaca gctttcacaa gccaatcaat gtgcttttct ttggcaagac
     2401 gtgaagccgt aatcaatgaa aatggtttgc gcccttgact tgaatctgtc aaggaatcaa
     2461 tactgcctac aggaatggta acaatctttg gctgatgctg agtatatttg gcaaattgct
     2521 cttgtagaac ttcattttgt ctatcagtag acacgataaa gaagtcaacc ttatctgcat
     2581 tggtaaactg atagtcataa tagttattcc aaaggatata gtcctcattt gtagcatttt
     2641 cactataatg ctccgcatga acaactaccg ctagatgtgc tgtctgtgct tcctcaaaca
     2701 caacctgtcc aatacctgtc tccctatcga gaatgaccaa atcagactta ttcaaattca
     2761 aagatttcat aaaggcacgc acaaaagctt gctttccata gaaaatctta tccttgaaat
     2821 gataaacttc ttccttccct tgattcatca agatatcata gactggagtc ccgtcttcat
     2881 tataaaaagt tcgttggtat aagactgcaa cattgtcctt gggagcaaaa tactcgctac
     2941 aataacgcgt ataagaaaag taatccttcc gaatcaggtt tcccttaaaa acatactcgg
     3001 catgttgaac caagtccttg ttctcatcaa ccaaataaca ggttacaaac ttatcttggt
     3061 caaaaaagaa tacacgtaaa accttgccat ttttttctct gtgactttct tcaccaccaa
     3121 agtaagccaa gacatcatcc actgtcacgc tagtaggtgc aattttgata tctgtgaaat
     3181 gattataaag ccagataacc tgattatcat caaaaccaat attggctgtt aagtgctgaa
     3241 tattatcggc taaaatcata tctgtaaaga taaacttaga ggacagattt aatttccgaa
     3301 aaacaccagc acgataggct tgagcgtatt caacaccgct actagcccaa ccaattccta
     3361 aatttatatt gtaaattgtc atattttctc cttttatgga aaatgagtca cgatttcacc
     3421 ttttggagtg acctcaacca gccccatcag gagattacgc accatatccg catgaatctg
     3481 ttctttcata gcttcaaagc ccgcgtaggc ttcttgatag tactctacga ttggattttt
     3541 ctgactagca gattgaccac cgatagccat ggatagctgt tgtagatagt ctacctgctc
     3601 tacccagttg tcatcaatgg ctttaagcag tgaaagtcgt aaaaactgtt catataagtc
     3661 atgttgatta agtaattctt tcttttcaga aagttcttta tcaatcacct gcttcataaa
     3721 gctacgaact gcagttttgt cagttacatc tatataatct ggaacctctt taacatgaaa
     3781 actaatattg gtcacaataa agtgaaacaa taattcacga ctagcatagt gatcagccgc
     3841 tacctcttct gtatatctct caatgatatc cacaacaaca tcctctaagt cacgagaacc
     3901 atctattaga cgatttctct ctttatagac tatatcccgt tgtatattca tactttcagc
     3961 atactccaga gtctgacgac gtgctgaacg tccagcacta tcactggcat gctgagcctt
     4021 ttcgactagt ttccggtatt tacgaccttt caatacttcc ggttgagtca tatcttgaac
     4081 ctgatagtct ttgtactttt tatgcaccca agatggacca aatttcttga taacatcatc
     4141 ctctaaggat acaaaaaatt tactcatacc aggatctccc tgacgaccag aacggccacg
     4201 aatttgtagg tcgatccgct gactttccat ccgctcagtc ccaataacaa tcaagccccc
     4261 aagctctgcg actcctttac caagcttgat atccgtacca cgtcctgcca tagaggtagc
     4321 cactgtcaca gcccccatct gacctgactc ggagataatc tgagcctcac gcgccgcatt
     4381 attagcattt aggacattat gggcaatccc ttcacgaaac aagagagacg aatagagttg
     4441 agacatttca actgagccta caaaaacgag taaaggattt cccttagcat ggtattgctt
     4501 gatgtactcc aaggatgcat acactttttc aggtaaagtg atatatagat tatctggata
     4561 gtcaatccgt tgtctcggac gattggttgg aatgcgtact acagacatat tgtaagtttc
     4621 aataaactct ttttccgcga ccttacctgt ccctgtcata ccagatatct tattaaacat
     4681 cttaaaaaga ctctgatagg tgatcgaggc catagcccgc gtctcaggag ataatttgac
     4741 atgttccttg gcttcaatag cctgatggag acctccttga agtttagtca tttccattag
     4801 acgccctgtt cccttatcaa ccagtaccat ctcatttcca cgaatgatat agtccttatc
     4861 tttagtaaag agcttatgag ctcgaatcgc ataaaccaaa tgacgagcaa aagacgcatg
     4921 ctcttccttg tataaattat caatccctag gaaattctca gcagacttgg cccccttagt
     4981 agtgagccaa acctcctctt tctcctcttt aaagatataa tcctctcctt cgaccaaggt
     5041 tgttacaagt gtatcaatga tcgcatagta attagactga acacgaggag aacccgcaat
     5101 aatcagagga gtttgtgcac tatcaagcaa gatatcatca atttcatcaa taatcacata
     5161 gttaaacggt cgtaaaaact taccttcttc attcgaggct aggttatcat ttagataatc
     5221 aaaacctaaa ttactattgg ttgtgtagat gatatccgaa gcatagataa gcttcttttc
     5281 ttcagctttc atctccttct ttggatcttc cgtaaatggt acaccaatgg tcaatcctag
     5341 aaaacgataa acttgaccca tttcctcggc atcacgcttt gataaatact cattaggagt
     5401 cacaaccatc actccttctc ctgaaaaagc gttcaaatag acaggcatgg tagctgtcaa
     5461 ggtcttacct tcccccgtat tcatctcagc aacatttcca tagtgcatga caatagctcc
     5521 catgacttga acatcataag gaaacatccc taaaatccgc ttatctgctt cacgcaccac
     5581 agcaaaagct tcaaccaaaa tatcgtctag actttctccc tcggaaagac gctgacgaaa
     5641 ctctactgtt ttagctacta attcttgatc cgaaagagag gacatcttgc ctttcagggc
     5701 attaatctgc tttaaaatct ttttcacttt cctaagctgg aaatcttgac ctaaacgtct
     5761 aaacacttct gatctccttg atagaaaaat gataaaaagt taaggactca aatccagcac
     5821 ttaaaagaga aactcgataa gtataactat cttctggata agtaaatatg aaatctttca
     5881 ctttttctac ctttttttct aaaatatcac catagcggtc aaaaaaagtt atcttggtat
     5941 aaactccatt ttcaggctga cagtcaaaaa ccagtgatag ttgatactcc tgctttcttt
     6001 ttaataaggg aagctgaggt tgggttcgtg cagcctggta attgacacta gaaaaccaag
     6061 tctttagaat ctcaccagat ggcaatagag gattatacag acgaacactt ttgtcagggt
     6121 aataagatac agttgttcca tacatataag tcccaccaac ttctccccaa tgaatagact
     6181 gtctctgtgt aataatcatc ttttctccct tccaaaacca gtctctaaga ccattctata
     6241 aaagtgcaaa aaccaagaaa tattggtatc tgtatcatca ttgtgtcgtc cagcagttcc
     6301 tttagataaa atcttcgcac ctgtattaca cagataagac actaattgtt cataggcttg
     6361 tggatccatt tcttcatctt tcatatagga taaaccaaag gttgtctttg aaaaatttgc
     6421 ttgtttaaaa attttccaaa aacgctgatc caactccgtc atatcttgag aacttgtccc
     6481 acctgtatga tgaattaaac aatcaaaagc taagttagag acacccggtg cgtccaaacg
     6541 tccacgacta gctatagttc ctaaattagc caagggctta ccaactatga tggcatgggg
     6601 ttcaaaagaa gcaccataat agagagccgg aaacgttccc atagaaagac ctgataaaat
     6661 caaatcctta tgatcaaagc ctaaataatc aagatagtga gtgatcgtat cctttacttt
     6721 tccctctagc tcatccgttc cgagataaaa agctcccccc tctaaacgtg gatcagaaaa
     6781 taaaatgaag ggacatccaa gagttttcat cataaagtaa ccctcgaatc cttctgcagg
     6841 tcgataacct gcaaaataga cagtcaaagg cggtttaaaa tcacctggat ggaagaaata
     6901 gtttatttca tcacgcttgg aatcatgtag gatatttcca ccaagtacaa acttaccaaa
     6961 ttgttttcga ctccatcgtt ggtgaagatt acctagtttg atagttccac gacctcttgc
     7021 ttcaacagat atcgagagaa agtagtctcc gtctcgacta tccataaaaa gtgcttgctc
     7081 caagtctttt tcagatacta ttacttcttc aaagaaatca tcaacagacc cactccacat
     7141 tttacggatt actaaacgaa agtcacaatt gccttccttc tcatattcaa gccacaactc
     7201 aatagggagt gttttttgca ccataatatt ataagcccaa taggcaagct gggcaaaatc
     7261 ttgcccaaac tcaccttcca aactgacata atccaatcct tgataagaaa tagctcctgt
     7321 aaaatttgga ttgacttgta tcgtcggagg aaagagttta tcaccatacc caccgccaaa
     7381 aagagattta cttaaatcat tgattagttg ttggggactt aaaaaatcaa ttgcttgtgc
     7441 acatcgtttt tttagaaaat ccacaacctc tctatcattt atttgtagat tctggttata
     7501 aaataaagta taaggttcaa aatattttac ataggaaaat aagtctctgg aatactgacc
     7561 atcttctatt aaaactgcat gaaaacggtt aatatcctct ttttcaatta tttcacgaag
     7621 ggcttttgat gagtttgggt aaaaataata ccaactcata ttttcaggta tgtcatagtg
     7681 attttcccaa ttggcaagtc ctatctgtaa aatattaagt tcctttgaca tttttaacct
     7741 cctctatcca tttctctaat tttccaagaa attgttgtcc tgtgtgctcc ttaatcttat
     7801 caattgaata tatcaaggac tcattccact ccttcaacct atctgtgtaa taatgtgcag
     7861 ccttagaaaa ttctgtaaca tctgctaaca aataaccatt ttttaaatgt tcaacataga
     7921 cggtttcaac taggttgatt tgaggaattc ctgcactaat cccagcaatc tgtgtgtaga
     7981 gatgaggatg tctatttaaa tccacaatca aacggacaaa ttctagtgtt tttatcaaat
     8041 ctaactcatc attcaagtta acaaactgta agcgtaagtc ctggtgttga ttttcttcca
     8101 gaggattttc tgcatcacca taatcaatcg cctttcccag attttcggat tgaatgtttt
     8161 cttggatgaa agactcaaca atcccttcaa cctcattcat ttgctcctga ctagcagcaa
     8221 aggctccaaa aatcacctca gtatccttat tttcagcgac aaaggacaag acttgaagca
     8281 gagcttgatt atcaatcccc tgctcaaaat ccagttgaaa ataaatgatg gattctttct
     8341 tagtttggct tcgtcccaat cgtaatcggg tgtcaaaaga tgataaatga taacattgat
     8401 gcattcgttc aggatacaac tcctgcaaca atcgtaaact atcctctcta tccaccaaaa
     8461 tcaaatccga tttttcaaaa gttacatcta aatccctaaa ggtatcttga ggattacgtc
     8521 caatgaacag actcaacagt ttaggattag tactaggtaa acgatctagt accaactggt
     8581 catgatgaga atgagaagga agcataaaaa tatcctgatc cttcacatac gtttgcagat
     8641 aattctcaaa aaattctgcc accagatctc ccatattttg ataggtaagc attttaaagc
     8701 gataacccaa aattggattg acttctacct gtcctccttc ttttaaacgc tctctgaatt
     8761 gccactctcc cttgaggttc agatattctt gataggctgc ttgcccatct tcaaaaaaga
     8821 taatgctcga tacaaaccca cgatcatcca cgatatggtg catatgtatt tgaccatttg
     8881 gttcaaaata ctgtatatca ctgatgaagc cttcaactcc atgttcaacc ttagcaaatt
     8941 tcttcccatt tttttgaacg ataatcgtaa agggactata aataaattca cagtcatcat
     9001 cccactcaat atctctaatg cttaaaactt gggtatggag attatgaaaa tcttgcataa
     9061 catcaaaaac ggaataagta tccatttcta acacaccatg tctatataaa aaataacgta
     9121 gatgaggctg gtaagctaat actaatagac gagaatctat atcttgctct tgaaagagcc
     9181 gaatctggtg aaaggtatca tcaaactcta gacgaaaatg agaaaaatac catggagtga
     9241 tatctgcatg ccatgttctt tctgacccat accaagctgg aataaaataa tacataaaag
     9301 tctcctaaat caaaggtcta taggtctcat ttagccttaa agccttgacc tcgtctctaa
     9361 tcgtaaaaat catgcccccg aacatcataa ataagcctgg aatcattgcc aatcgtaata
     9421 acttttcatc aaacaaaaca aaaagcatgg gaccacctgc cataatcaca ttaaagagac
     9481 cacctatgac tgagaaacga aggaccaatc gattaataaa tcgactagta tccgcacctg
     9541 gataaatacc ataaatgtat tctccagatt ttttcatacg gtctgcaatc tcttctccat
     9601 tcatcgtaac aaaagcaaaa atgatactaa ataaaaataa gaccgaaata taaacataga
     9661 cccacaaagg ctttccaacc ataaattcct ttgataaagc cgctaaccct gaatgattag
     9721 ggaaaataaa tcccaacaag atgaacaaat aagctggtac actaagaaaa ctcatcacat
     9781 acatataagg catcccacct gcaggattca acatgatttc gagataagaa tagcgtttaa
     9841 atcgattgtg taagccaatt ttattaacag gaaccaaata gcgagctcga tacataaggg
     9901 caagtaaata agaaaagaca agggttaata aagcaagtaa cacaataatc cctgttggaa
     9961 tgtgtactgt ctgaaatgtt tccaaaacat cctgaggaat atttaaaacc atactggata
    10021 ggaggattac aatagaacct ccaatcccca tactcgcatt taaatctgac aaccaaacaa
    10081 gaaaaaatgt tcctgctatc agcaatattg tattcattag aacaaccaat attgcagaat
    10141 aggaggattg tactggcagt ctcaagctaa ctgccaagga ttgaatcaca gcaattagca
    10201 aggtcaggta cattttacgg cgatcttgta tttctataga cgtagatgtt aaacccaacc
    10261 gtttagaaaa agaaaacatc tgccataaaa tcatggcgga catccaaggg gataatccaa
    10321 cagaaaaaat tgataaactt cttagattcc cacctgttag ggcggctgag aaggctagat
    10381 aggctgttga acctcctaaa aaatctttag tatttaggtc aacaaaaggg agaataatac
    10441 gacttcccaa tacatagata aacagtagaa atgaggtaaa tagtcctttt tttactgcta
    10501 ttgacgaata aatcttagtc ataaaacact cccctcacct tcattgtttg tgatatggta
    10561 aataacaatt cttttatttc aacttttacc atttctttgt tctgcatcaa aaaacaactt
    10621 taatctgaaa caaatatctt tatattcctc agtatctgtt aattgatggt attctaaagt
    10681 ttgcttataa tcttttaaaa gattgacaaa tcgaatacgc agaacggaga catccaaacc
    10741 agccaagaca caatccgata ttttcttaga aaacacttca acaatatctc ttgcccagga
    10801 aagatcaaat tccgttctca tgatgcttcc tgaacgtctt ctatacagat agagattatc
    10861 gtttataaag acgattttag aggctaaaag ataaaagcga tgcatagttg cttcatcatc
    10921 aaagcggcga ccatttgaaa agtgaacttc attgaataat tcttttttaa tcaacttaaa
    10981 tgtcggcaat ataaaggccg agctattgaa tttccaatct cctgcttgac ggttcataat
    11041 ttcttgtata gctaattctt cgacgcaaaa atcttgccct gttacataaa aataaaaatt
    11101 cccgtcactt tcgtcataat tatagtaatt ccctataaca acatctgcat ccttttcttg
    11161 gacttgctga taaagagttt caatcatatt ttcttttacg atatcatctg aatcaacaat
    11221 cgtgatgtac tctcccttag ctacactaat ccccatattt ttagcttgtg ctgccccccc
    11281 ttattttctt gatgcaatac tcgcacacga ttatcttgca tcataaaagc atcacaaatt
    11341 tccccagaat tatccgtaga accgtcatca actaaaataa tttctatatt ttgataggtt
    11401 tgcttcagaa tatgctcgac acactccact aaatattttc ccgtattgta tatcggtacc
    11461 acaacactaa ttaattcact cattactcta tctctatatt cttcattcta tccactaaat
    11521 catctggtct ctccacagaa aaaataccgt catagagtcc atcatccata cttttacgtg
    11581 tgatatcaaa agcgaagatt ttcttgccac tttcttgtgc ccttgtaact acattaaaaa
    11641 cctctccacc tgtattaata tctaaataaa ctatagaatc gtccaatagc caatcaattc
    11701 tactatgtaa tacattttga tatactgtta cattcgtata ctgtgataga gaggtcagct
    11761 cctcagaaca atcacacggt gcagccaaat gaaaatgcca atcaggtaac cgttgtacta
    11821 aatactctaa atgttttata tctgcactcc atgtcacaag catcacttgt tttttagact
    11881 gattgcttct ttcaaaataa tttaaatcgg aacgttgaaa agcaatctct gaccaatcca
    11941 aatctctata aacccaccat aattcacgta gtctagatat actatatgta ttccaaggtt
    12001 tatcatgact agcatagtga acaattgtag gtggattgtc atctagacgt tcacattctt
    12061 gagcaaggtg ataaatatca atacccacca tataattata tgttttatct aaggctagcc
    12121 aattatcctc aaaataaata tttaaaatac tctgatcccc aagatgaaca acttgattct
    12181 gctcggccgc taattccaat aaactattga caatagaatg ttctttccac tttgcaacat
    12241 ccattagtaa cataccagta ttaaatccag attttcgtcc ttcataggca tagacatcat
    12301 caacagcacc aattgaatat cctttgagat ctatctcaaa caaagtagct agttccccag
    12361 ttacaatgat atcggaatcc agatacaata ccctatcaga ttcaaccact tctgtcgcaa
    12421 aaaatctaaa gtaagaagca taatttatat gaggtcctgt tttatagctt tcaaaaagtt
    12481 ctttatcaat gtgtacactg cgaattgtag aattcacaac ttccattttt ctccccaata
    12541 atttaaacca ttcaggagct atatcactgt tgagaatata aaaatcaaca tctctattgt
    12601 gatacaatac agactttata gtcgtctcta aaggaataag ataggcatta tctgccgcta
    12661 atactattga ttttctcata ctgttctcta cctcatgtaa gatctgattg ctgcaaccat
    12721 ttttttcgga atggagcttg gatagatacc ttcatatgaa atctcagaaa gattttcaga
    12781 gcaagtattg tcgaaagcta tcattggttt tttgtactta agcacaaact catatgcatc
    12841 ttctaatttt cgatcatgat tcaaatccaa atacaaatct gaagctatta ctatttgttc
    12901 caataacatt ggtaaggaat ttggatatac ggtaacatta ggaaaacgcg ttaaagctag
    12961 tagttcattt gccatatttg tataagcaac aatatgaaaa tgaacttctg gcaattgctc
    13021 cgccaaataa tcgattttct cgacacacca agaattcgtt aaattaacac attgcaactt
    13081 aaatatttgt gatttactag ggtacttcac actctttgaa aaccattcat ttaaaataac
    13141 agaccaatcc atcaaagagt attcccacca aacttctctt agacgtccaa cagaaaattg
    13201 attccaaggc ttatcctgag aaatatagtg taaaattagt ggcagtggtt cgagcggaat
    13261 atcaaaaatg aattgatgtt taaaggttgc cgccccataa tcatatccta tttgaaaatt
    13321 atattgatct tcaagggaac tatattgatc tttaaacaac atattcaaaa ttgactggtc
    13381 tccttcttcc acattctcat gttctttttc tgttaagtca atcaattttt gtcgaatagt
    13441 ttcagatccc cattttttgt tgttaatcaa gagaacacca gcattgaagc cgactcctgc
    13501 tccaaagcaa gaacgagctg ctgccaaata attttcacct aagtctaatt caaacaaatc
    13561 ggtcaaatca ccagtcacaa tcaaatcact atctagatag agaactttat cttctgttac
    13621 aaaatctgga ataaaatagc gtgcaaatgt catatgattg atatgaggta atttattaga
    13681 ccaattcatt tgaaactgtg agccaattaa cttgcagtca atcaagtcgc cccccatctc
    13741 ttgtaaatat attcttattt gactaaacca ttcctgagga atgtcctgat ttagcagata
    13801 aattttcaaa tgactattgt gtctacagag tgacttcatc gccgtttcga tttgtcgaat
    13861 ataagcgtaa tcacctgcaa atactacagc gcgtttggta tttctcattt taaatcctta
    13921 tcatttattt taaatagtct agtttaatat tcgtgcaaca atctgactgt tataggtttc
    13981 atcatcaata aattcaatat cttggtcgaa attatgttct gcagtatgtt tatgagaaaa
    14041 tttaacctta gttttagctc ccattttcat ccattcatac tctgagtcaa tatggcctac
    14101 atccaaaact tgatagccca tctggcatag attataactc agaacttttg ctgtaggtcc
    14161 aagcatacat aaaatcaagc gaccaccagc atacttttca atttcttgtt caagttcatg
    14221 aactctagaa aaggcactat gagaaggaca gataattcgc ttaatagaat ttgcctcatc
    14281 gaataaatca tttccgacac ctgaacgaga agtcgcacct tcgactatca gtaagtcacg
    14341 gttttcccaa atgcttttca atttttcaaa ttgagcttta gcttgactct tgtcttcaaa
    14401 atcgatataa gggcgagata caaaggttga gccataccat gaatcactgc ataactctct
    14461 gtaaaaatcc atataatgat ccatgtgatc tttccaaaat ggaatcgccc acgatgtaaa
    14521 cctaaaacga tctgtaaaag catcaggaag gcacactact aaatcttctc gactttcttg
    14581 gccgataatg tccctcatga ttgaaaccaa ctcttcatca taatcctggt agggaattga
    14641 atgccctgca agcatattga tttccccatc tccaaaacga actaaagaag agttgtgttc
    14701 aataatataa tccaaggttt catctatccc tttcacctgg attatatcaa gcggtttttc
    14761 tagcatatcc ttagttatca atttttgcaa atcagccaac attcgttcag gatgttcact
    14821 tgaatacaac ctttggccat tattcccatt ttgagattta taaaaggcta gtataggttt
    14881 agatagatga gccatttctg caacgatatc ggatgttgaa gtcagtaggt tgatatctaa
    14941 ataagcatcc atcttctcct tcaactcgtc tagcttatct ctagaaattg ccggatagag
    15001 ctgaatatta ggataacgat tagacaaatc atataatttt ggccccatag ctgtccaagc
    15061 agcaatttga aaaacaacat ttggcaactg tccagccaag taatccaatt gttcgatata
    15121 ctgagaagcc gtaaacgtta aacaagttaa acttggaagt tgtcgaaaac aggtaagatg
    15181 tccgtttttc ttaaaatctt taatcgtatc aatcataaag gaaactttcc cattttcgaa
    15241 aacgatttgt cctaactctc cgtgctgagt cgattgaaaa gctagtagcg ttttttcttg
    15301 ttcttgcaaa gattttaaaa agttctcatc tgcactacca tgattgatat ccaaatatag
    15361 atttgttgac tttttcagct tatctaagac cggtggaaca atttgtggat gcaatctcac
    15421 attattatat acagctaatt tttttaattt atctcccata tccgtccaag ctgcgatatg
    15481 aaataccacc tcaggtagag ctgtaactag ctcttctatt ccttctaaat cttgggaatt
    15541 cgttaaggtt aagcaagaaa attccttatc tagaggcgat attagttcaa attctcccat
    15601 atggtgttgt aaaatctgac tccactccaa atcatggaat tcccaccata aatcacgata
    15661 acgattggct gtcaaggtag tccagggttt tctgtaggtc gtaaaatgaa tcaccactgg
    15721 tttatcatta aaagccagat gttcctgcca attgttatac aaagccacaa tatcatgccc
    15781 tacttgtaaa ttataagctc gacctagttc taaccaatca tcttgcaaga cctgattaaa
    15841 aatcgtttga tcaccattaa aatgctcgaa acggccttct tcaacttcct tcattgtaac
    15901 aatgctctgt tcaattagtc gttcttttaa tttctcttga cgccattttt tattgttgat
    15961 taataaaaca cctgcattaa atgtaattcc atctgtatct ttaactgctg cgagtgattt
    16021 ttcttctaaa caaatactaa ataatttctc taaagaagta tttacaatca aatcactgtc
    16081 taaatataaa accttatctt cttggatata atctgcaata aaatatctag cataagtaat
    16141 gctactaatg tgatcttgct tttcccaatc ttgaaacaca gtttgttcag gtagtttaac
    16201 atcgataatc tcactaccta acatgcgagc tatttttcgt ggtttgcgaa accaatctgg
    16261 catgatatct tgattcaaaa tataaatctt aacatctcga ttgtggtata aaatagattt
    16321 tatcgttgtt tctaactgcc tggtgtaatt gcgatcccct gctagtacaa ttgttttgtt
    16381 catttcattc ccttaatcaa ttcccaagtg atagattgca tctaccaata actttttagt
    16441 gaaatagccc tcttttaaca aatagctaaa ggtcttgata cgattcgtca tttcttgata
    16501 ttcttgtaga ttcattttat caactatctc atgaacctct tccagactat ccgccataaa
    16561 gcccaagcct tgatctacta taaatttagc agttgacaag ctacttggta caatgactgg
    16621 aatgcccgct gttagatagg tactcacctt atgagatata ttcaaggtat agtattggtt
    16681 actttctccc tcattttgat gggttcccca gacaaggcca aatccaccct ttgataattc
    16741 tagcaacaat tcctcatctt ttttccatcc ttcgatgctg agacttctag cactagaact
    16801 agcttcccct ttatttgaaa atactctcaa aggcgtatct tgagaccaat tttgtaagtc
    16861 tggaaaacgc tctaaacttc cagcaaaaaa aagttctttt ttaaaagcag gggtgtataa
    16921 ggataaatca tgaggatgat cccacatccc ttgaacaaga atcttcttag tcgtcaatcc
    16981 ttcttccatc aggcgtgttt tcattctctc tgacggcact atcaaaacat ctgatagatt
    17041 atacatatac agataatctt tcatgagata atagttacta tcaaacatga ggggaacaac
    17101 atcatggata aagcaaataa ttttcacctg catatccttt agcttatcaa acaagagacg
    17161 atcaaattca aaaccattcc aggttggaga ctgaaagact aaaatatccc caatagagat
    17221 actggccata ataccatcca gacgcttatt catttcagaa ggactatctg aagcaatgtt
    17281 gtaaaaataa ataccaacct ctctaaatcc cagttgacta gctatctttt gaacagcatt
    17341 ttgagctaag ataaccgtac tatcaccagc catgccgtat aaatttgtta aatgtagttt
    17401 catattacgc tatctcattt tttattacta tctacggaag taaagacaat ttttccatca
    17461 ttcttctgta aatctctgtg aactgcatat tttgttcttc taattttgct atcactcttt
    17521 gtaatctatt tttataaatc aaaatatgat tggtcaagtc atagtctgaa cttgctaaaa
    17581 tagctatctt ttcttcccta gccccaattt catcatacat acgcttttca ctccatgtat
    17641 tcgataaagt atcagataaa ccaacacggt accagtaaac acaacgattc aaatagacta
    17701 tcctcgaagc tcttagaagt accttccatg tccagtaagt atcctctgca atttttccta
    17761 taggaaatgg taaatcttgt agtaactctc tcttgaataa cttcaagaca gctacagtcc
    17821 agttcccatt tctgacttct tcgacacctt ccctatccat aattgcttta ccttctatca
    17881 cttctagaga atcatctgga tccgtaacat aagtcatata cacatagcgt gtttcatcat
    17941 aagaattata acgcccgata ctaatatctg cgttttcctt tttcaaagca ccatataatc
    18001 ggtctaaagc atcatgttcc aaccaatcat cagagtctac aaaagtaatg tacgcccccc
    18061 cccgaacatt caatacctag gttacgagct gatgaaagac cgccgtttgc tttctcaaaa
    18121 tatttgaaac gagaatcttt ctctacaaat tcttcacata ttttggatga atgatctgga
    18181 gagccatcat tgattaataa acactcaaaa ttttgatacg tctgattctg aatgctatcc
    18241 aaacacattc gcaaataatt ctcaacgttg tagattggaa ctacaatact aattagttcg
    18301 tctgccataa acttattctc ctataaattg tctaatagcc tctaccattt tgtttggttc
    18361 ggttgaagaa aatatattag tttgattgcc tatatcatga ctagtatttt caaaggtaaa
    18421 aattggttta gatagttgtt gaacaacgga tacaatatta tccacttctt tataatgatt
    18481 aatatctaaa taaaaatcta aattatccaa aatttttcga taatcaaatg gagaaaaaca
    18541 aggataaatc gtaacattgc tatatcttaa taaagcaacg acactagacg caaaatatgt
    18601 atgtgctagt atagaaaaat gtacctctgg taaattttct atcaaatatt ctacatgctc
    18661 catctcacaa ctagctgtaa aaattgctgt atgagcttta ggactgtata caagttcttc
    18721 gaaactacga ctaataatgt cttttctcaa taaaatatca gaccattcta acagattata
    18781 aaaccaccaa acttctctaa agcgattatt ggaaattatt tcccaaggtt ttaccccagt
    18841 ataatgtata acactaggta aatctccatt tttcaactca gaaatctcat accatttatg
    18901 atttccttct atgtgtgcga cgctatccat ccccaccata aaattaaaat ttcggtctaa
    18961 tcttttccat ctatcatgga ataacatatt taaaattcct tgatctccat atgctgtttc
    19021 atgatattga ttggtcagtt ctaacagttt cgaacaagca tcttcatctc tccaagtatc
    19081 tacattaact aataacattc cggagttaaa attggtagaa ggaacatcac caaaagaatc
    19141 ttctactgct gccaaggcat aaccatctag ttctatatca aataaatagt ctaaacttcc
    19201 tgtaacaatg atgtcagaat ctaggtataa agcacgactt tctttgacaa aattaggtat
    19261 aaaataacga aagaaagtgg cataacttaa attctttaac ggtaaatgaa actttttaag
    19321 aacatgatct acaatcttta cattaacgat ttcagattgt atagtcttca atcgcttatt
    19381 catcatcaaa aaccactctg acgcaatatc atcattaaat acataaaatt ttaaacaatt
    19441 attataacaa caaatagatt taattgcagt ttctaattta tctacataat cattatctgc
    19501 attaaaaact atagattttg tattcattaa ca
//
LOCUS       DAVKNL010000037        19493 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVKNL010000037 DAVKNL010000000
VERSION     DAVKNL010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19493
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.037"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            635..1018
                     /locus_tag="VW411_001343"
     CDS             635..1018
                     /locus_tag="VW411_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEU9794065.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            1070..1759
                     /locus_tag="VW411_001344"
     CDS             1070..1759
                     /locus_tag="VW411_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9794066.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFSLSQLTLIVQNYWQFSSQIGNLF
                     WIQNILSLLFIGVMIVVLVKTGHGYLFRIPRKKWLWYSILTVLVLVFQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYIALFFLVPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFVFATVGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            1801..2034
                     /locus_tag="VW411_001345"
     CDS             1801..2034
                     /locus_tag="VW411_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794067.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            2185..2796
                     /locus_tag="VW411_001346"
     CDS             2185..2796
                     /locus_tag="VW411_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9794068.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMLYNSWPLLYYL"
     gene            2957..3751
                     /locus_tag="VW411_001347"
     CDS             2957..3751
                     /locus_tag="VW411_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEU9794069.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            3748..4383
                     /gene="trmB"
                     /locus_tag="VW411_001348"
     CDS             3748..4383
                     /gene="trmB"
                     /locus_tag="VW411_001348"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEU9794070.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            4509..4988
                     /gene="rimP"
                     /locus_tag="VW411_001349"
     CDS             4509..4988
                     /gene="rimP"
                     /locus_tag="VW411_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEU9794071.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            5032..6168
                     /gene="nusA"
                     /locus_tag="VW411_001350"
     CDS             5032..6168
                     /gene="nusA"
                     /locus_tag="VW411_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEU9794072.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            6190..6483
                     /locus_tag="VW411_001351"
     CDS             6190..6483
                     /locus_tag="VW411_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEU9794073.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            6476..6775
                     /locus_tag="VW411_001352"
     CDS             6476..6775
                     /locus_tag="VW411_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEU9794074.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            6792..9668
                     /gene="infB"
                     /locus_tag="VW411_001353"
     CDS             6792..9668
                     /gene="infB"
                     /locus_tag="VW411_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU9794075.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAAPKASAEKKAE
                     KSEPVKPAVAKEEAKPAEPVTPKTEKVAAKPQSRNFKAEREARAKEQAERRKQNKGNN
                     RDQQQNGNRQKNDGRNGGKQGQSNRDNRRFNDQAKKQQGQQKRRNERRQQEDKRSNQA
                     APRIDFKARAAALKAEQNAEYARSSEERFKQYQAAKEALAQANKRKEPEEIFEEAAKL
                     AEQAQQVQAVVEVVPEKKEPAVDTRRKKQARPDKNRDDYDHEEDGPRKQQKNRSSQNQ
                     VRNQKNSNWNNNKKNKKGNNKNNRNQTPKPVTERKFHELPTEFEYTDGMTVAEIAKRI
                     KREPAEIVKKLFMMGVMATQNQSLDGETIELLMVDYGIEAKQKVEVDNADIERFFVED
                     GYLNEDELVERPPVVTIMGHVDHGKTTLLDTLRNSRVATGEAGGITQHIGAYQIVENG
                     KKITFLDTPGHAAFTSMRARGASVTDITILVVAADDGVMPQTIEAINHSKAANVPIIV
                     AINKIDKPGANPERVIGELAEHGVMSTAWGGDSEFVEISAKFNQNIEELLETVLLVAE
                     IQELKADPTVRAIGTVIEARLDKGKGAVATLLVQQGTLNVQDPIVVGNTFGRVRAMTN
                     DLGRRVKVAGPSTPVSITGLNEAPMAGDHFAVYEDEKSARAAGEERAKRALMKQRQAT
                     QRVSLENLFDTLKAGELKSVNVIIKADVQGSVEALSASLQKIDVEGVKVTIVHSAVGA
                     INESDVTLAEASNAFIVGFNVRPTPQARQQAEADDVEIRLHSIIYKVIEEMEEAMKGM
                     LDPEFEEKVIGEAVIRETFKVSKVGTIGGFMVINGKVARDSKVRVIRDGVVIYDGELA
                     SLKHYKDDVKEVTNGREGGLMIDGYNDIKMDDVIEAYVMEEIKR"
     gene            9919..10269
                     /gene="rbfA"
                     /locus_tag="VW411_001354"
     CDS             9919..10269
                     /gene="rbfA"
                     /locus_tag="VW411_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEU9794076.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTIIDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
     gene            10548..11327
                     /locus_tag="VW411_001355"
     CDS             10548..11327
                     /locus_tag="VW411_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794077.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELDPKDIPTL
                     LEQGPTTLLSQEILDRVANACIRDNVLLASGTSVLAGLPGGLAMAITIPADVAQFYAF
                     SLKLAQELGYIYGYEDLWASREELSEDAQNTLLLYLGVMLGVNGTAALLRVGSITIAK
                     QVMKIVPNKALTKTLWYPILKKVLKIFGVNLTKGGLAKGMGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIKGE"
     gene            11329..11511
                     /locus_tag="VW411_001356"
     CDS             11329..11511
                     /locus_tag="VW411_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794078.1"
                     /translation="MNPIKAFAKIYGNYFLTVQGVKVMKTIKKADHVVVGLGKLFIAD
                     KLMDTARWLIKPEERE"
     gene            complement(11866..11940)
                     /locus_tag="VW411_001357"
                     /pseudo
     CDS             complement(11866..11940)
                     /locus_tag="VW411_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            11971..12204
                     /locus_tag="VW411_001358"
     CDS             11971..12204
                     /locus_tag="VW411_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEU9794079.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            12204..13538
                     /locus_tag="VW411_001359"
     CDS             12204..13538
                     /locus_tag="VW411_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEU9794080.1"
                     /translation="MTDERIHILRDILLELHNGASPESVQDRFDATFTGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYESMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKGLREGSKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            13548..13802
                     /locus_tag="VW411_001360"
     CDS             13548..13802
                     /locus_tag="VW411_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEU9794081.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKVGKGFHDLPEGLFGERNY"
     gene            13898..14293
                     /locus_tag="VW411_001361"
     CDS             13898..14293
                     /locus_tag="VW411_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEU9794082.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            14706..15155
                     /locus_tag="VW411_001362"
     CDS             14706..15155
                     /locus_tag="VW411_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEU9794083.1"
                     /translation="MIGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEK
                     LERLGVHYEISERTQTPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPELLDE
                     KGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            15142..15702
                     /locus_tag="VW411_001363"
     CDS             15142..15702
                     /locus_tag="VW411_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9794084.1"
                     /translation="MARTELPDKIETERLVLRVRTVVDAEDIHTYASLPEVAYPAGFP
                     PVKILEDEIYYLEHILPERTQKENLPAGYGIVVKGTDKVIGSVDFNHLHADDVLELGY
                     TLHPDYWGRGYVPEAACSLIDLAFKELGLHKIELTCFGYNLQSQRVSEKLGFTLEARI
                     RDRKDAQGNCCDDLRYGLLRSEWEVI"
     gene            15702..16280
                     /locus_tag="VW411_001364"
     CDS             15702..16280
                     /locus_tag="VW411_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9794085.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYQLMYLKQIQDLLDNFHQEI
                     LFVELETSLEECIRRNRTENRLKHKPLKRHIEVSEREILETAETLQLNSQHQPNELHH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            16273..18924
                     /locus_tag="VW411_001365"
     CDS             16273..18924
                     /locus_tag="VW411_001365"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEU9794086.1"
                     /translation="MSKELSPKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ITRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VNLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPELGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDDKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAAYPTVNLAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIYLPLADLLNVEEELARLDKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     ECDKQADYQAKYDATVARIDEMKKLVK"
     gene            19005..19169
                     /locus_tag="VW411_001366"
     CDS             19005..19169
                     /locus_tag="VW411_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEU9794087.1"
                     /translation="MGKIVAIDLFNGAGGTTSGLKKSGIDVQVAVEIDSVAVKTYKLN
                     NPEVSVIDME"
     gene            19220..>19493
                     /locus_tag="VW411_001367"
     CDS             19220..>19493
                     /locus_tag="VW411_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9794088.1"
                     /translation="MSEYWFSTNVDQIDEVDGKQCLIYSYYNIKASRNVEVLKGRSGT
                     KKGLDYWEPYAPQKQYEMERLPKNKYIGSSSTDRWDGIEKNVVFCDC"
BASE COUNT         5900 a         3625 c         4426 g         5542 t
ORIGIN      
        1 atgcttactt tttaaatttt ttaacaattc aagatgtttc gatgacaatt caagatttgg
       61 atgaaaaatt ttaaaaaaca atgatatact aaacttgtca aagttgcaac gggtgctgct
      121 acattatgtt aaaaatcgct cttgattgat aagctggatt tgttaggaaa ggagtaaata
      181 attatggata agaaaaagat agtttcgact ataatatgta tagtatttct tgtagtgtca
      241 gtagataatt tttttagaga cttaaccccg ttactgttca ttttaaatat tattggcctg
      301 tcttgctttt cagtgctaac atacattaaa ataaaagaga tactcttaaa tataagtaaa
      361 tgatgatttg gttagtccta gaaccaatca acttatgttt atcggcttgg cagattttat
      421 gtttgtcatt tgtttgtata gaggtatttc agaaacagaa ttctatcaac aactaatcgc
      481 ttatataggt gtattctctg cttgtttatc acgcttctgc tcatgtgggg cttgaagtat
      541 tataaaaagt agctttctat ccttttctga caggatattt gagtagaagg ctctattaaa
      601 ttgttagtga aataaatgaa tggaggtatt taatatgaaa tacagattat tttttgttat
      661 tttcttgagt agtgtgttgg atattctttt agggacattt ttacaaatct ctatcgtatc
      721 tattgggtgg cttgttcttt acagtggttt gtttgaagca ggagttttcc ttcttgctaa
      781 taaaggggtg gcggtaaaaa tcaaggaagt agatattcga aatcgcttta aatttatttt
      841 tggaaaaacc ttatggtttc aaattctttt gctcatcttt ttgataatca aactttatct
      901 tggtttggat gcgaggttga ttttattcta tggacatatt ttcattgtct ttaatgcctt
      961 aatgtatcta ttatctagta gtcaggttag ccttaaaaaa aacaaactgt cttcttaatc
     1021 ttacagcagt agagcctgac aaggtgaggt catagtaggg aggatttcta tgaaaaagta
     1081 tcaacttcta ttcaaaataa gtgcagtctt ctcttactta tttttcgtat ttagtctttc
     1141 tcagctgacg cttatcgtcc aaaactattg gcaattttct tctcagatag gcaatttatt
     1201 ctggattcaa aatatcttga gtttactttt tattggagtc atgattgtgg ttcttgttaa
     1261 gacaggccat ggttatctct tccgcattcc aagaaaaaaa tggctttggt attcgatttt
     1321 gacagtatta gtgctagtgt tccagatctc ttttaacgtt cagacagcta aacatgttca
     1381 gtcaactgcg gaaggttggg ctgtattgat tggttatagt gggactaact ttgcagagct
     1441 aggtatttat atagccctgt tctttctggt tccactgatg gaagaattga tttatagagg
     1501 attactgcaa catgctttct ttaagcattc gcgatttggt cttgatttgc ttcttccttc
     1561 tattttattt gctctccctc atttttcaag cctgcctagt ctgttagata tcttcgtctt
     1621 tgcaacagtt ggaatcatct ttgctggttt gacccgctat accaagagca tttatccatc
     1681 ctatgcggtg catgtgatca ataatattgt agcgaccttc ccgtttttgc tcacttttct
     1741 acatagggtc ttggggtaaa aaaacaaaag gcttgctttt cagccataga ggaggtcatc
     1801 atgtataaac acttattttt cctagattcc aaaactttag atcggttgac accctatatt
     1861 ctagtcttgg cttctgacac cattgctttt aatgtttttg tgctaacctt tgtatctgcg
     1921 gtggttttta atttcctaaa ttccatgcta gctttaatgg ctatattcat aggggctggc
     1981 tatgtggtcg gattttggtt actaatactc aatgaaaatc aaagagcaaa ctaggaaact
     2041 agccgcaggc tgtacttgag tacggcaagg cgacgttgac gtggtttgaa tttgattttc
     2101 gaagagtata aaatggtttg ttttggaaag gctagaacta aagaatgacg tgtaggaagt
     2161 ctgtttgttg aggaggataa ttttatggag ttttttgata aatttcatgc cttgtgtttt
     2221 ggatttttag tactaataat tgtcattaca gttccttata cgattaacca tgggggtttt
     2281 tttcaaaatg aatctgcatt gattcttgta agtcttcttg taacctcgct gagtgttgct
     2341 tatgctagaa agtttgaaat gatttctttt gggatgttaa gcaagaaaca acttttgctt
     2401 ttcattgcaa tctttcttct aagtgtactt gagacgctgg tttatattca tttcttcgct
     2461 gtttcttctg gctcaggggt ccaacacttg gcggaagtca gcagaggaat ttccctgtct
     2521 ttgattttga ctacctcagt ttttggcccc atccaggagg aactcatttt cagaggactt
     2581 cttcaaggtg cggtttttga caattcttgg ttagggcttg tgctaacttc ctctctcttt
     2641 tctttcatgc atggaccttc taatgtccct tcgtttattt tttatctact tgggggtttg
     2701 ttgctgggct ttgcttataa aaagagtcaa aacctatggg tttctactct agtccatatg
     2761 ctttacaaca gttggccact cttatattat ttataaaaat tatgaaaagg taagcagaag
     2821 acggtgctta cctttttcaa gctataatga tacccaagcc aatccagagg cttttctttg
     2881 agaatcgggt tagagtggtt gacgaatagg ccaaaaacta gtagaatagt aaggaaactt
     2941 tatacggagg aaagaaatgg atttgggtga taatgagcta acactgactc ccatacctgg
     3001 gaaaagtggc aaggcttata tgggtagcta tcctgatggg aagcgcatct ttgtaaaaat
     3061 gaacacctct ccaatcctac ctggtctagc tagagaacaa attgctccac aattattatg
     3121 gagtcgtcgt ttggcagatg ggcgtgatat gtgtgctcaa gaatggttga caggcaagat
     3181 attgaccccc tatgatatga atcgtaagca aatcgtcaat attttaaccc gtcttcatcg
     3241 ctcacgtccg ttgatgacac aattgagtcg tttgggctat gccatggaaa cacctgtaga
     3301 tttactacag tcttggcagg aaacggctcc agatgctttg cgtaaaaatc attttatcag
     3361 tgaagtgatg gctgatttac gtcagactat tccaggattt agagaggacc atgcgaccat
     3421 tgtccatgga gatgtacgac atagtaattg gattgagaca gatagtggct tgatttattt
     3481 agtagattgg gattcggttc gcttgaccga tcgcatgttt gatgtggccc atatgctctg
     3541 ccattatatt tcagaacatc agtggaagga atggttgacc tactacggtt acaagtacaa
     3601 tcaaacggta ttaagtaaat tgtattggta tggtcaattg tcttatttga gtcagatttc
     3661 caagtattat atgaaccaag atttagaaaa tgtcaatcgg gagattcatg gtttgcgtca
     3721 tttccgagac aagtatggaa agagaagatg agagttagaa atcgtaaagg ggcaacagaa
     3781 ttactagagg caaatcccca gtatgtggtc ctcaatccct tggaagccaa ggcaaaatgg
     3841 cgggacttgt ttggcaatga taatcccatt catgtggaag ttggaagtgg aaagggtgcc
     3901 tttgtttcag gtatggccaa gcaaaaccct gacatcaact atatcgggat tgatattcaa
     3961 aagtctgttt tgagctacgc tttggacaag gtgcttgaag ttggagtgcc taacatcaag
     4021 ctcttgtggg tagatggttc tgacttaact gactactttg aagacggtga gattgatcgc
     4081 ttgtatctga acttttcaga tccatggccg aaaaaacgcc atgaaaagcg tcgtttgacc
     4141 tacaagacct tcttggatac cttcaaacgt atcttgcctg aaaatggaga aattcatttc
     4201 aagacggata accgtggctt gtttgagtac agtttagtga gcttttctca atatggcatg
     4261 aaactcaatg gtgtctggtt agatttgcat gccagtgatt ttgaaggcaa tgtcatgaca
     4321 gaatacgagc aaaaattctc aaacaagggg caagttatct accgagttga ggcagaattt
     4381 taagagataa cctaaaatta ggctgtacaa gtgcttttgc tttacataag ttggcaaacg
     4441 tgctatactg atagtaagaa tatgaaaagt gaggcgggga aatatcttcg cctcttgctt
     4501 atgaggaggt ggacgcaatc gcaacaatcg tagaattagt cagagaagtt gtagaacctg
     4561 tcatagaagc tccttttgaa ctcgtggata tcgagtatgg aaagattggc agtgacatga
     4621 ttctcagtat ttttgtagat aaacccgaag gaattacctt gaacgacacg gcagacttga
     4681 cagaaattat cagtcctgtc ctagacacca tcaagccaga tcccttccca gaacaatatt
     4741 tcctagaaat taccagtcca ggtttggaac gtcctttgaa aaccaaggat gccgtcgctg
     4801 gagcggttgg aaaatacatc catgtcgggc tctaccaagc catcgataag caaaaggtct
     4861 ttgaaggaac cttgttggcc ttcgaagagg acgagttgac tatggaatat atggacaaga
     4921 cgcgtaagaa aaccgtccaa attccataca gtttagtatc aaaagcacgt ttagcagtta
     4981 aattatagaa aaagaaagga tagcttttga ggattcaaaa gtgaagaaaa catgagtaaa
     5041 gaaatgctag aggccttccg cattttggaa gaagacaagg gaatcaaaaa agaagatatc
     5101 atcgacgcag tagtagagtc gcttcgttcc gcttatcgca gacgctatgg tcagtcagac
     5161 agcgtagcta ttgacttcaa cgaaaaaaca ggtgacttta cagtttatac tgtccgtgaa
     5221 gttgttgatg aagtatttga tagccgtttg gaaatcagct tgaaagatgc tcttgccatt
     5281 aattcagctt atgaacttgg agacaaaatc aagtttgaag aagcaccagc tgagtttggt
     5341 cgtgtagcag cccaatctgc caaacaaacc atcatggaaa aaatgcgcaa gcaaacacgt
     5401 gccatcactt acaatactta caaagaacat gagcaagaaa tcatgtctgg tacagtagaa
     5461 cgctttgaca accgctttat ctatgtcaac cttggtagca tcgaagccca attgtcaaaa
     5521 caagaccaaa ttcctggaga agtttttgct tctcatgatc gtatcgaagt ttatgtttac
     5581 aaggttgaag acaaccctcg tggtgtgaac gtctttgtta gccgtagtca tccagaaatg
     5641 atcaaacgtt taatggagca agaaattcca gaagtttatg atggaactgt tgaaatcatg
     5701 agcgtggctc gtgaagcagg tgaccgtacg aaggttgctg ttcgtagcca caatccaaac
     5761 gtggatgcta tcggtacaat cgttggacgt ggtggtgcta atatcaagaa gattactagc
     5821 aaattccacc cagctcgtta cgatgctaaa aatgaccgca tggtaccaat cgaagaaaat
     5881 atcgatgtta tcgagtgggt agcagatcca gctgaattta tctacaatgc catcgctcct
     5941 gctgaggttg accaagttat ctttgatgaa aacgacagca aacgtgcctt ggtggttgtt
     6001 ccagataaca agctttctct tgccattggt cgtcgtggac aaaacgtgcg cttggcggct
     6061 cacttgactg gttaccgtat cgatatcaag tctgctagcg aatttgaagc catggaagac
     6121 gctgcttcag tagagttgga agtagaaaac gatactgtag aagaataaaa gctgctagag
     6181 gagggaaaga tgaaaacaag aaaaatccct ttgcgcaagt ctgttgtgtc taacgaagtg
     6241 attgataagc gtgatttgct ccgcattgtc aagaacaagg aaggacaagt ctttattgat
     6301 cctacgggca aggccaatgg ccgcggcgct tatatcaaac tagacaatgc agaagcccta
     6361 gaggcgaaaa agaagaaggt ctttaaccgc agctttagca tggaagtgga agaaagcttt
     6421 tatgacgagt tgatcgctta tgtggatcac aaagtgaaaa gaagagagtt gggacttgaa
     6481 taagcaaaag ataagtaatc tcttggggct tgctcagcga gcagggcgca tcatatcggg
     6541 tgaagaattg gtggtcaagg ccattcaaga cggcaaggcc aagttggtct ttctagctca
     6601 tgatgctgga cccaatctga ccaagaagat tcaagataaa agtcattatt atcaagtaga
     6661 aattgtaacc gtgttttcaa cactggaatt aagcatagca gtcgggaaat cgagaaaggt
     6721 tttggctgta acagatgctg gatttacaaa gaaaatgagg tctcttatgg aatagaagag
     6781 gaggacatga tttgtctaag aaaagattgt acgaaatcgc aaaagaactt ggaaaagaaa
     6841 gtaaagaagt tgtagcgcgt gcaaaagagt tgggcttgga tgtgaaaagc cactcatcaa
     6901 gtgtggaaga agctgtcgct gcaaaaattg ctgccagctt taagcctgca gctgctccga
     6961 aagtagaagc aaaacctgca gccccaaaag taagtgcaga aaagaaagcc gaaaaatctg
     7021 agccagctaa accagctgta gctaaggaag aggcaaaacc tgcagcccca aaagcaagtg
     7081 cagaaaagaa agccgaaaag tctgaaccag taaaaccagc tgtagccaag gaagaggcaa
     7141 aaccagctga gccagtcact ccgaaaacag aaaaagtagc ggctaaaccg caaagtcgta
     7201 atttcaaggc tgagcgtgaa gcacgtgcta aagagcaggc agagcgacgc aagcaaaata
     7261 agggcaataa ccgtgaccaa caacaaaacg gaaaccgtca gaaaaacgac ggccgtaatg
     7321 gtggaaaaca aggtcaaagc aaccgcgaca atcgtcgctt taatgaccaa gctaagaagc
     7381 agcaaggtca gcaaaaacgt agaaatgagc gccgtcagca agaggataaa cgttcaaatc
     7441 aagcggctcc acgtattgac tttaaagccc gtgcagcagc cctaaaagca gagcaaaatg
     7501 cagagtacgc tcgttcaagt gaggaacgct tcaagcagta tcaggctgct aaagaagcct
     7561 tggctcaagc taacaaacgc aaggaaccag aggaaatctt tgaagaagcg gctaagttag
     7621 ctgaacaagc acagcaagtt caagcagtgg ttgaagtcgt ccctgagaaa aaagaacctg
     7681 cagtggatac acgtcgtaaa aaacaagctc gaccagacaa aaatcgtgac gattatgatc
     7741 atgaagaaga tggtcctaga aaacaacaaa agaatcgaag tagtcaaaat caagtgagaa
     7801 atcaaaagaa tagtaactgg aataacaaca aaaagaacaa aaaaggcaat aacaagaaca
     7861 accgtaatca gactccaaaa cctgttacgg agcgtaaatt ccatgaattg ccaacagaat
     7921 ttgaatatac agatggtatg accgttgcgg aaatcgcaaa acgtatcaaa cgtgaaccag
     7981 ctgaaattgt taagaaactt ttcatgatgg gtgtcatggc cacacaaaac caatccttgg
     8041 atggggaaac aattgaactc ctcatggtgg attacggtat cgaagccaaa caaaaggttg
     8101 aagtggataa tgctgacatc gaacgtttct ttgtcgaaga tggttatctc aatgaagatg
     8161 aattagttga gcgtccacca gttgttacta tcatgggaca cgttgaccac ggtaaaacaa
     8221 cccttttgga tactcttcgt aactcacgtg ttgcgacagg tgaagcaggt ggtattactc
     8281 agcatatcgg tgcctaccaa atcgtggaaa atggtaagaa gattaccttc cttgatacac
     8341 caggacacgc ggcctttaca tcaatgcgtg cgcgtggtgc ttctgttacc gatattacga
     8401 tcttggtcgt agcggcagat gacggggtta tgcctcagac tattgaagcc atcaaccact
     8461 caaaagcagc taacgttcca atcatcgtag ctattaacaa gattgataaa ccaggtgcta
     8521 acccagaacg cgttatcggt gaattggcag agcatggtgt gatgtcaact gcttggggtg
     8581 gagattctga atttgttgaa atttcggcta aattcaacca aaatatcgaa gaattgttgg
     8641 aaacagtcct tcttgtggct gaaatccaag aactcaaagc agacccaaca gttcgtgcga
     8701 tcggtacggt tatcgaagcg cgcttggata aaggaaaagg tgcggtcgca acccttcttg
     8761 tacaacaagg taccttgaat gttcaagacc caatcgttgt cggaaatacc ttcggtcgtg
     8821 tccgtgctat gaccaacgac cttggtcgtc gtgttaaagt tgctggacca tcaacaccag
     8881 tctctatcac aggtttgaac gaagcaccga tggcgggtga ccactttgcc gtttacgagg
     8941 atgaaaaatc tgcgcgtgca gcaggtgaag agcgtgccaa acgtgccctc atgaaacaac
     9001 gtcaagctac ccaacgtgtt agccttgaaa acctctttga tacccttaaa gctggggaac
     9061 tcaaatctgt taatgttatc atcaaggctg atgtacaagg ttctgttgaa gccctttctg
     9121 cctcacttca aaagattgac gtggaaggtg tcaaagtgac tatcgtccac tcagcggtcg
     9181 gtgctatcaa cgaatcagac gtgacccttg ccgaagcttc aaatgccttt atcgttggtt
     9241 tcaacgtacg ccctacacca caagctcgtc aacaagcaga agctgacgat gtggaaatcc
     9301 gtcttcacag cattatctac aaggttatcg aagagatgga agaagctatg aaagggatgc
     9361 ttgatccaga atttgaagaa aaagttattg gtgaagcggt tatccgtgaa accttcaagg
     9421 tgtctaaagt gggaactatc ggtggattta tggttatcaa cggtaaggtt gcccgtgact
     9481 ctaaagtccg tgttatccgt gatggtgtcg ttatctatga tggtgaactc gcaagcttga
     9541 aacactataa agacgacgtg aaagaagtga caaacggtcg tgaaggtgga ttgatgatcg
     9601 acggctacaa tgatattaag atggatgatg tgattgaggc gtatgtcatg gaagaaatca
     9661 agagataaga ttttttgctc ctttcttagg tggtgaggga cgcaagcaaa ccgatggttt
     9721 cattgcttat ttttgagcct agggtctcaa aaatcccctg tgatgggact gataaatcag
     9781 ttccatcact ttcaccacgg cgaaagaagc agatgacttc aaattgaact tcgtttcaat
     9841 ttaaactgaa aatcaagaag tttaaaatag ctaggtctgc tggcctagct tttggttcaa
     9901 agtagagaaa ggaatatcat ggcaaatcat ttccgtacag atcgtgtggg catggaaatc
     9961 aagcgtgaag tcaatgagat tttgcaaaag aaagtccgtg atccacgtgt ccaaggtgtg
    10021 accatcatag atgttcagat gctgggtgac ttgtctgttg ccaaggttta ttacaccatt
    10081 ttgagtaacc ttgcttcgga taaccaaaaa gcccaaatcg ggcttgaaaa agcaactggt
    10141 accatcaaac gtgaacttgg tcgcaatttg aaattgtaca aaatcccaga tttgaccttc
    10201 gtcaaagacg agtccatcga gtatggaaac aagattgacg agatgctacg caatctggat
    10261 aagaactaaa gaagaggggt tgcccctctt ttttggtgga ggaaaatagg ttgaatttga
    10321 aatggaaaaa tattctttta taatagattg aaactagaat agtacgcctc tacttctaaa
    10381 atattgttag aaatcgattt gactgtcctg atcgatttgt cctgttcttg tttcatttta
    10441 atataaaaaa gggattctgt attttttaat gttatctaat tagaaaatgc tttttttgta
    10501 ggaaatataa tatgataagg tgcaaaaaag aaataaggag tttgtatatg gctgaacaag
    10561 acttagctat gcaagtattg caacaagtgg tgaaactacc tgttgttaag gttgatcgtt
    10621 cgaaattttt agtggataag ttttccaaag aattggatcc aaaagatatt cctaccttat
    10681 tggaacaagg tccaacgact cttctatctc aagaaatatt agatcgtgta gctaatgctt
    10741 gtattcggga caatgtatta ttagcgagtg ggacttctgt tttggcagga ttacctggag
    10801 ggcttgctat ggcaattacc attccagctg atgtggctca attttatgct ttctctctga
    10861 aattggctca agaattaggt tatatttatg gttatgagga tctttgggct tcacgagagg
    10921 agttgagtga agatgctcaa aataccctct tgctttatct aggcgtaatg ttaggggtga
    10981 atggaaccgc tgctttgcta cgtgttggta gtataacaat tgccaaacag gtaatgaaaa
    11041 tagtgcctaa taaagcttta acaaagacgc tttggtaccc tattttgaaa aaagtcttaa
    11101 aaatatttgg tgtgaatctt accaagggag ggttggccaa aggaatgggg aaatttattc
    11161 ctatcttggg tggtatcatt tcaggtggtt taacctttgc aactatgaaa ccaatggggg
    11221 aaagcttgca gaaagaatta tccaagctag tcaactatag tgaagttcaa tatcaagaag
    11281 atgttgaaac aatccgaaaa gaggctgaaa tcatcaaagg agagtaatat gaatcctatc
    11341 aaagcttttg ctaaaattta tggtaattac tttttgaccg tgcaaggtgt aaaagtgatg
    11401 aaaacgataa agaaagctga ccatgtcgtt gttggtctgg ggaaactttt tattgccgac
    11461 aagttaatgg atacggctcg gtggctcatt aagccagagg agagagaatg aaattttttt
    11521 ggtcttcttg ctattctttt tatcaaaccg attattggga ttgtgaaatt cttttggatg
    11581 atcatctctt ttgcagtcca attgctgttt tacaagatag tgtttaagat attggattgg
    11641 ctctttaaac ttatctagat ggtaatccaa gttgcagaga actagcagga actccactgc
    11701 tagtttttta ttctctttcc atatggtata atataagcag taaaatcatt ttatactctt
    11761 cgaaaatctc ttcaaaccac gtcagcttca ccttgcagta tatatgttac tgacttcgtc
    11821 agttctatcc acaacctcaa aacggtgttt tgagctgact tcgtcagttc tatctacaac
    11881 ctcaaaacac tgttttgagc aacctgcggc tagcttccta gtttgctctt tgattttcat
    11941 tgagtattag aacatacaat ggaggtcgtc atggacaata tcatcgatgt gtcaattcct
    12001 gttgcagaag tggtggacaa gcatccagaa gtcttggaaa ttctagtgga gttgggtttt
    12061 aaaccccttg ccaatccctt aatgcgcaat acagttggtc gtaaagtatc acttaaacag
    12121 ggttctaagc tagcaggaac tcctatggac aagattgtac gcacactgga agcgaatggc
    12181 tacgaagtga ttggattaga ctaatgacag atgaacggat tcatatccta cgggatattt
    12241 tgttagaatt gcacaatggc gcctctcctg agtcggttca agatcgcttt gatgcgacct
    12301 ttacgggcgt gtcagccatc gagatttccc ttatggagca cgagctgatg aactcggatt
    12361 cgggcgtcac ttttgaagat gttatggaac tctgtgatgt ccatgccaat ctttttaaaa
    12421 atgctatcaa aggtgtcgaa gtttcagata ctgagcatcc aggtcaccca gttcgtgtct
    12481 tcaaagaaga aaatctggct ctccgtgcgg ccttgattcg cattcgtaga ttgttagata
    12541 cctatgagtc tatggaagac gaggaaatgc tggcggagat gcgtaagggt ttggtgcgtc
    12601 agatgggact tgtgggtcaa tttgacatcc attaccaacg taaggaagaa ctcttctttc
    12661 ctatcatgga gcgctatgga cacgattcac ctcccaaagt tatgtgggga gtggatgatc
    12721 agattaggga actctttcaa acagctctaa cgacagccaa gtcactacca gaagtgtcaa
    12781 ttagcagtgt aaaggaagct tttgaagctt ttgcgacaga gtttgaaagt atgattttca
    12841 aggaagagtc catcctcctc atgattctcc ttgagtcttt tactcaggat gactggcttc
    12901 agattgcgga ggagagcgat gcctatggct atgccatcat ccgtccgtca gagaaatggg
    12961 tgccagaacg acagagcttt attgaggaaa agattgcaga ggagcctgta cagctagata
    13021 cggcagaagg tcaagttcaa caagtcatag atacgccaga aggccatttt accattacct
    13081 ttacccctaa ggaaaaggaa gctgtgctgg accgccatag tcaacaggct tttggtaatg
    13141 gctatctttc agtcgagcag gccaatctca tcctcaatca tctccctatg gagattacct
    13201 ttgtcaataa agaagatatt ttccagtatt acaatgacaa tacgccagct gatgagatga
    13261 ttttcaaacg gacgccgtcc caagtcgggc gcaatgtcga actctgccat ccgcctaagt
    13321 acttggacaa ggtcaaaact atcatgaagg ggcttcgtga gggaagcaaa gacaagtatg
    13381 aaatgtggtt caagtctgag tcgcgaggta agtttgtcca catcacctat gctgcagtac
    13441 acgatgaaga cggagaattc caaggagtgt tggagtatgt tcaggatatc cagccctacc
    13501 gtgagattga tacggactat tttcgtggat tagaataagg agaaaaaatg agttacgaac
    13561 aagaatttat gaaggaattt gaagcttggg tcaataccca aatcatgatt aacgacatgg
    13621 cgcacaagga aagccaaaaa gtttacgaag aagaccagga cgagcgtgcc aaagatgcca
    13681 tgattcgcta cgagagtcgc ttggatgctt atcagttctt gcttggtaag tttgaaaact
    13741 tcaaagtagg caagggattc catgatttgc cagaaggctt gtttggtgag cgaaattatt
    13801 aaacgagaaa gattcttgat ttttcactaa aatcttgata gaatgtttat gttaaatcct
    13861 tgtcagagca gggatttttt attgaaagga ttttatcatg tcaaagaaac tcaatcgtaa
    13921 aaaacaatta cgaaatggcc tccgtcgcgc aggtgccttt tcaagtacgg tgactaaggt
    13981 tgtagatgag acaaaaaaag tcgtgaagcg tgcagaacag tcagcaagcg cagctggtaa
    14041 ggctgtttct aaaaaagttg aacaagcagt agaagctacc aaagagcaag ctcaaaaagt
    14101 agctaattct gtagaagatt ttgcagcaaa tttgggtgga cttccacttg atcgtgccaa
    14161 gactttctat gatgaaggaa tcaagtctgc ttcagatttc aaaaactgga ctgaaaaaga
    14221 actccttgcc ttgaaaggaa tcggcccagc taccatcaag aaattgaaag aaaatggcat
    14281 caagttcaag taatttttct tgagccttgc atttccgaaa aaatcttgct acaatagagc
    14341 cattagaggt gttttgaatc ccacatttta cagaaagtgg cggcgctgag aagtccacaa
    14401 atgtgtcaaa actggttgct aatggatgaa aaattgaaat aaaagtgtct ttttgcttta
    14461 aagacgagag ttgcgggcaa ctgcccgaaa ttgggtggta ccgcggataa acacattcgt
    14521 ccctgtcatg tagatggcag ggcttttctt ttacgtctta gtcaaagggg gttgttatga
    14581 agcaatcttt taacacaagt aaactatatt atggttttcc tgtttttatt ttaggatatc
    14641 aagattagaa ttttggctat aacatcacga cctgtagttc ctcttatagt ctaggagatt
    14701 gggttgtgat tggagtcgtt gcgagagaga atgccgcaga gcagatcaaa cagtatcaaa
    14761 aatttactgt gaatatttct gatgaaactt ctatgcttgc gatggagcag gctggtttta
    14821 tcagtcatca ggagaaattg gaacgtttgg gagtgcatta tgaaatttct gaacgaactc
    14881 agactcctat tttagacgcc tgtccacttg ttttagattg tcgggtagat aggattgttg
    14941 aggaagacgg tatttgccac atctttgcca agattcttga gcgacttgtt gccccagaac
    15001 tcctggatga aaagggacat tttaaaaatc aactgtttgc cccaacctat ttcatgggag
    15061 atggatatca gcgcgtttat cgctatctgg acaagcgtgt agatatgaag ggcagtttca
    15121 tcaaaaaagc gaggaagaag gatggcaaga actgagctgc cagataaaat cgaaacagaa
    15181 cgtctcgttt tacgagtccg cacagtggtg gatgctgagg atatccatac ctacgctagt
    15241 ttgccagagg tcgcctatcc agcaggtttt cctccagtca agatcttgga agatgagatt
    15301 tattatctgg agcacattct tccggagcgt actcaaaagg aaaatctccc agctggctat
    15361 gggattgtcg tcaaaggaac cgataaagtc attggttctg ttgacttcaa ccatcttcat
    15421 gcggatgatg tgctagagct tggctatacc ttgcacccag actattgggg tcgaggttat
    15481 gttccagaag cagcgtgttc cttgattgat ctagctttta aagaacttgg tcttcacaag
    15541 attgaactaa cttgctttgg atataacctt caaagtcaac gagtctcgga aaagcttggc
    15601 tttaccctcg aagctcgcat aagagaccgc aaagatgctc aaggcaactg ctgtgatgat
    15661 ttgagatatg gcttgctgag gagtgagtgg gaggtgattt gatgcatctt ttcatcattg
    15721 gtgctccagc atcaggaaaa atgaccattg gtcaagaatt atctcgactg acggatgcta
    15781 ccctatttta taaccatcaa gccattgatt ttgcactaga gatctatcag gattataccg
    15841 aggagatgtg ggaatttgtt cgtggaatta ccttttcttt ccttggagca agtgcaagaa
    15901 atcagcgatc agtaatttta acagacgtaa ttgatttttc aaatcagtac cagctgatgt
    15961 atttaaagca aattcaggat ttattggata actttcatca agagattctg tttgttgaat
    16021 tggaaacaag tcttgaagag tgcatacgtc gaaatcgaac ggagaatcga ttaaagcaca
    16081 aacccttaaa acgacatatt gaggtatctg aaagagaaat tttagagacc gctgaaacac
    16141 ttcaattaaa ttcccaacat caaccgaatg agttgcacca ctactttaaa ataaataata
    16201 cgaatttgtc tgcagaagaa gctgctaagc aaattcaaaa taaaataaac aaaatagaga
    16261 aaggacacac acatgtctaa agaactttca cctaaataca atccagccga ggttgaggct
    16321 ggtcgttacc aaaaatggct tgatgcggat gttttcaagc cttcaggcga tcaaaaggct
    16381 aagccttatt caatcgtgat tccaccacca aacgtaactg ggaaacttca ccttggtcac
    16441 gcttgggata cgactttgca ggatatcatc atccgtcaaa aacgcatgca aggctttgat
    16501 accctttggc ttccaggtat ggaccacgct ggtattgcga ctcaggctaa ggttgaggag
    16561 cgcttacgtg gtgagggtat tacgcgttat gacctaggtc gtgagtcttt cttgacgaag
    16621 gtctgggaat ggaaagacga atatgccact accatcaagg aacaatgggg caagatgggg
    16681 ctttctgtag actattctcg tgagcgtttc actcttgatg aaggtttgtc aaaagctgtt
    16741 cgtaaggtct ttgtgaacct ttacaagaaa ggctggatct atcgtggtga gtttatcatc
    16801 aactgggacc cagcagctcg cacagccctt tctgatattg aggtgattca caaggatgta
    16861 gaaggtgcct tctaccacat gaattacatg ctggaagatg gttcacgcgc ccttgaagtt
    16921 gctacaactc gtcctgagac tatgtttggg gacgttgcgg ttgcggttaa tccagaagac
    16981 ccgcgctaca aggacttgat tggtaaaaac gtcatccttc caatcgctaa taaactcatc
    17041 ccaatcgttg gagatgagca cgcagatcct gagcttggta ctggtgtcgt gaaaatcaca
    17101 cctgcccacg atccaaatga cttcttggtt ggccaacgtc ataacttgcc acaagtcaac
    17161 gtcatgaacg acgacggaac tatgaatgag cttgcctttg aattttcagg catggatcgt
    17221 tttgaagctc gtaaggcagt cgttgctaag ttggaagaaa tcggtgccct tgtcaaaatc
    17281 gaaaaacgtg tccacagtgt tggtcactca gaacgtacag gtgtcgtagt tgagccacgc
    17341 ttgtctactc aatggttcgt caagatggac caattggcta agaacgccat tgctaaccaa
    17401 gacacagagg acaaggtcga attctaccca cctcgtttca acgatacctt ccttcaatgg
    17461 atggaaaatg tccacgactg ggttatctct cgtcagcttt ggtggggtca ccaaatccct
    17521 gcctggtaca atgctgatgg tgaaatgtat gtcggcgaag aagctccaga aggtgacgga
    17581 tggactcagg acgaagacgt cttggatact tggttcagtt ctgccctctg gccattttca
    17641 accatgggct ggcctgaagt cgactcagaa gactttaaac gttatttccc aacttcaacc
    17701 ttggtaacag gttacgacat catcttcttc tgggtgtctc gtatgatctt ccagtccttg
    17761 gaatttactg gtcgtcagcc attccaaaac gtgcttatcc acggtctcat ccgtgacgag
    17821 caaggacgca agatgtctaa atctctcggt aacgggattg acccaatgga tgtcatcgag
    17881 aaatacggtg ctgatgccct tcgttggttc ctttcaaacg gttccgcacc aggtcaagac
    17941 gttcgtttct cttacgagaa aatggatgct tcatggaact ttattaacaa aatttggaac
    18001 atttctcgct atatcctcat gaacaatgaa ggtttgacgc tggatgtggc gcatgacaat
    18061 gtcacaaaag ttgcaacagg tgaggctggt aatgtgacgg accgctggat tctccacaat
    18121 ctcaacgaaa ccattgcaaa agttactgaa aactttgata agttcgagtt tggtgtggct
    18181 ggtcatatcc tttacaactt catctgggaa gaatttgcca actggtatgt tgagttgacc
    18241 aaggaagttc tatacagcga caatgaagac gataaggtca ttactcgatc tgttctcctc
    18301 tatacgctgg acaagatcct tcgtctcctt cacccaatca tgccattcgt gacagaggaa
    18361 atctttggac aaatctcaga aggttctatc gtgacagctg catacccaac tgttaatcta
    18421 gcctttgaag accttgcggc tcacactggt gtggaaagtc tcaaagactt gattcgtgct
    18481 gttcggaatg cgcgtgcgga agtaaatgta gcaccaagca agcctatcac catccttgtt
    18541 aagacaagcg atagcgactt ggaagccttc tttaacagca atgtcaacta tatcaaacgc
    18601 tttacaaatc cagaacactt ggaaatcgca tcaaccatcc ctgcacctga actggctatg
    18661 tcaagtgtca tcacaggagc agaaatctac ctgccactgg ccgatctcct caatgttgaa
    18721 gaagaactgg cccgtctcga caaggaactt gctaaatggc aaaaagaact ggacatggtt
    18781 ggtaagaaac tctctaacga acgcttcgta gccaacgcca aaccagaagt cgtccaaaaa
    18841 gaatgcgaca aacaagctga ctaccaagct aaatatgacg cgaccgtcgc acgtattgat
    18901 gagatgaaga agttggtgaa atgatgttaa aaacctaacc ctatcgggtt gggttttaca
    18961 tttgataatg ttataataaa aaggagaaac tttaggagaa tactatgggg aaaattgttg
    19021 ctattgattt atttaacggt gcaggtggca ctacaagtgg ccttaaaaaa tcaggcatag
    19081 atgttcaagt tgctgttgaa attgattccg tagctgttaa aacttataag ttaaataatc
    19141 ctgaggtgtc tgtaatagat atggagtaaa taaaacttca attttacaag ctttatatgg
    19201 ttctccagcc aataaaagtg tgtctgaata ttggtttagt acaaatgttg atcaaataga
    19261 tgaagtggat gggaaacaat gtttaatcta ttcatattat aacattaaag cttctagaaa
    19321 tgtagaagtt cttaaaggcc gttctggaac aaaaaaagga ttagattatt gggaaccata
    19381 tgctcctcaa aaacaatatg agatggaaag attacccaaa aataaatata ttggaagttc
    19441 ttcgacagac agatgggatg gcattgaaaa aaatgttgtc ttttgtgatt gta
//
LOCUS       DAVKNL010000038        18959 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVKNL010000038 DAVKNL010000000
VERSION     DAVKNL010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18959
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.038"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            119..934
                     /locus_tag="VW411_001368"
     CDS             119..934
                     /locus_tag="VW411_001368"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEU9794089.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIQAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNTEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
     gene            953..1594
                     /locus_tag="VW411_001369"
     CDS             953..1594
                     /locus_tag="VW411_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEU9794090.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKGYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKIIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            1625..2626
                     /locus_tag="VW411_001370"
     CDS             1625..2626
                     /locus_tag="VW411_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEU9794091.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITVAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEELFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSSTSIENLLANAQDIIR"
     gene            2636..3265
                     /locus_tag="VW411_001371"
     CDS             2636..3265
                     /locus_tag="VW411_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEU9794092.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYITLR"
     gene            complement(3644..4029)
                     /gene="tnpA"
                     /locus_tag="VW411_001372"
                     /pseudo
     CDS             complement(3644..4029)
                     /gene="tnpA"
                     /locus_tag="VW411_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(4413..7613)
                     /locus_tag="VW411_001373"
     CDS             complement(4413..7613)
                     /locus_tag="VW411_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HEU9794093.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTIGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSADASTRVIEAKDGA
                     ITISSHEKLRAALHRMVPIEAKKKYKLRFKIKTDNKIGIAKVRIIEESGKDKRLWNSA
                     TTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQLSEDSQTD
                     KQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKEV
                     KKIPLKILASVKDAYTDRLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSIS
                     SQADRTYLWEKFSNYKTSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSMEW
                     MHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDPE
                     HFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGFY
                     QDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAPL
                     LVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQCLQSLVKTIVQS
                     DSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRPSYLSAFNKMDKTAMYNAEKGFGFG
                     LSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTET
                     DAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFLG
                     SNIQNTSTDTAATTIDQRKLESGNPYKVYVNDKEASLTEQEKDYPETQSVFLESFDSK
                     KNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNRDSYGYML
                     IPNVDRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVLK
                     RGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKNH
                     SDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            7836..8312
                     /locus_tag="VW411_001374"
     CDS             7836..8312
                     /locus_tag="VW411_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEU9794094.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLQDQKSGPLGKRVEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            complement(8534..10747)
                     /locus_tag="VW411_001375"
     CDS             complement(8534..10747)
                     /locus_tag="VW411_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEU9794095.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSQTGSFEDRTTQLIYNRDFGQ
                     ISLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVQTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(11148..11324)
                     /locus_tag="VW411_001376"
     CDS             complement(11148..11324)
                     /locus_tag="VW411_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794096.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            complement(11703..13049)
                     /gene="celB"
                     /locus_tag="VW411_001377"
     CDS             complement(11703..13049)
                     /gene="celB"
                     /locus_tag="VW411_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEU9794097.1"
                     /translation="MDQQNGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFTSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNPLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NVVARFGTTGIFTAIIMAIVTVLIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVV
                     AFVVIFINGLLVAMGTDIFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGAN
                     IVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQL
                     KAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPI
                     IAQVPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
     gene            complement(13129..13635)
                     /locus_tag="VW411_001378"
     CDS             complement(13129..13635)
                     /locus_tag="VW411_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794098.1"
                     /translation="MDKKERQKIEQQRREMALTNTFFNRYLLLRYSIALFFFGNIYWL
                     LSQFISPSPIIIFPIMLIVFSILATVEQFKLYGNRKEKLGITLMFVRIQMLISIGLLV
                     LTWTSWFKNLFPIFENNQVARLFVFVVLLLGLVLSLLDIRRIKKIYKRTDKAYQQFVQ
                     LEKNSLSL"
     gene            complement(13666..13980)
                     /locus_tag="VW411_001379"
     CDS             complement(13666..13980)
                     /locus_tag="VW411_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEU9794099.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAKEMLALYKK
                     VN"
     gene            complement(13990..15969)
                     /locus_tag="VW411_001380"
                     /pseudo
     CDS             complement(13990..15969)
                     /locus_tag="VW411_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101767.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
     gene            complement(16087..16401)
                     /locus_tag="VW411_001381"
     CDS             complement(16087..16401)
                     /locus_tag="VW411_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEU9794100.1"
                     /translation="MAKALIICAAGMSSSLMAQKTTEFLKGKGKDIVVDAISATEGGK
                     AIAAAAFDLYLVSPQTKMYFKQFEEAGSKAGKPVVQIPPQAYIPIPMGIEKMANLILE
                     NI"
     gene            complement(16531..16770)
                     /locus_tag="VW411_001382"
     CDS             complement(16531..16770)
                     /locus_tag="VW411_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family sugar transporter"
                     /protein_id="HEU9794101.1"
                     /translation="MIGSIAAILTTFAFLPQVFRVVKTKDTGSIALGMYVMQVIGIAL
                     WLAHGIRIGDLPLILANSVSFLLSGIILFYKLKYK"
     gene            complement(16822..18258)
                     /locus_tag="VW411_001383"
     CDS             complement(16822..18258)
                     /locus_tag="VW411_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEU9794102.1"
                     /translation="MTTFKDGFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVA
                     REITLGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSIAWTRIFPKGDELEP
                     NEEGLQFYDNLFDECLKNGIEPVITLSHFEMPYHLVTEYGGWKNRKLIDFFARFAEVV
                     FKRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYEEGDNREAIMYQAAHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPVTCNPKDILMAMKAMQKRYYFADVHVLGKYP
                     EHIFKYWERKGISVDFTAQDKEDLLGGTVDYIGFSYYMSFAIDSHRENNPYFDYLETE
                     DLVKNNYVKASEWEWQIDPEGLRYALNWFTDHYHLPLFIVENGFGAIDQVAADGMVHD
                     DYRIEYLGAHIREMKKAVVEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDN
                     GKGSYNRSPKKSFGWYKEVISSNGESVE"
     gene            complement(18448..18786)
                     /locus_tag="VW411_001384"
     CDS             complement(18448..18786)
                     /locus_tag="VW411_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEU9794103.1"
                     /translation="MTLTIHVYYKGDGDSAIKFAREMLDSGLVKEIRNQKGNLKYEYF
                     LPIEKEGTILLIDQWINQETLDKHYQSKTMQKILDLRKKYHLQMQVERYIEDDSGMPE
                     SDKSFIDTGN"
BASE COUNT         5924 a         3933 c         2964 g         6138 t
ORIGIN      
        1 tttttttagg aaaacaaacc tttttataaa taaaaatatt tatttattga caaaaccggt
       61 ttactagaat ataatttggt tatagagaag tcttaacaaa tttgaaagga agtatcaaat
      121 gacaaataca tcattctcaa ttgagcagtt ttctttaaaa ggaaaaattg ctctcatcac
      181 cggcgcttct tatggaattg gatttgctat tgccaaatcc tacgctgaag ccggcgctac
      241 tattgtcttt aacgatatca atcaagatct ggtcaataaa gggattgaag cttatcgtga
      301 agttggcatc caagcccatg gatatgtctg tgacgtgaca gacgaggacg gtatccaagc
      361 catggtcaag caaatcgaac aagaggttgg tgtcattgac atcctcgtta ataacgctgg
      421 tattatccgc cgagttccaa tgtgcgaaat gagcgccgct gatttccgta aggtcatcga
      481 tattgactta aacgcaccat ttatcgtttc aaaggcagtt attccttcta tgataaagaa
      541 agggcatgga aagattatca atatttgttc gatgatgagc gaactgggac gtgaaacagt
      601 tagcgcttat gctgctgcta aagggggctt gaaaatgttg acccgcaaca ttgcgtctga
      661 atacggtgga gccaatatcc aatgtaacgg aattggaccg ggttatattg ccactcctca
      721 aacagcacct cttcgtgaat tgcaagaaga tggttctcgc cacccatttg accagttcat
      781 cattgcaaaa acacctgctg cacgttgggg aaatactgaa gatttgatgg gccctgctgt
      841 ctttctcgct agtgatgcca gcaattttgt caatggccac atcctatatg tagatggcgg
      901 tatcttagcc tacatcggaa aacaacctga gtaaaaatag aaagaagatc ttatgaaaat
      961 cgcattaatc aatgaaaata gtcaagctag caagaatcac attatttacg atagtctaaa
     1021 agaagcgaca gataaaaaag gctaccaatt atttaactat ggtatgcgtg gagaagaagg
     1081 agaaagtcaa ttaacttatg tgcagaacgg actaatggct gccatccttt taaatacaaa
     1141 ggcagttgac tttgttgtta ccggctgtgg tacgggtgta ggggctatgc ttgctttaaa
     1201 cagcttccct ggtgttgtct gtggtctagc agtggaccca actgacgctt acctttattc
     1261 tcaaatcaat ggtggtaacg ccttgtctat cccttatgcc aaaggatttg gctggggggc
     1321 agaactgacc ctcaaattga tgtttgaacg cttatttgct gaagaaatgg gcggtggcta
     1381 cccaagagaa cgtgtaatcc ctgaacaacg caacgctcgt atcttaaacg aggtgaaaca
     1441 aatcacccac aatgatttga tgaccatcct taaaataatc gaccaagact tcctcaaaga
     1501 caccatctct ggcaaatact tccaagaata cttctttgaa aactgccaag atgatgaagt
     1561 tgctgcttat ttgaaagaag tattagccaa gtaaagctat tctaaaccag aaaggaacta
     1621 atggatgacg aaaatattac tgtttggcga accattaatt cgaatttcac cattagatgc
     1681 caccagtatc ggcgatcatg ttgccagttc gacttatttt ggcggatcag aaattaacat
     1741 cgcttgtaat ttgcaagccc tgggtatctc aacgaaagtt tttaccgcac tccctgccaa
     1801 cgagattgga gatcgttttc tcacattctt gaaacagcac caaatcgata ccagttcaat
     1861 ctgtcggctt ggcgatcgaa tcggcctcta ctatttggag aacggctttg gttgtcgtca
     1921 aagtgaagtt ttctacgatc gtaagcatac gagtatcagc cagattcggc caaacatgct
     1981 agatatggat tctctctttc aggggattag ccattttcat tttagtggaa tcaccgtagc
     2041 tatcggtcaa gaggtccgtg cgatccttct cctactcttg gaagaagcca agcgccgagg
     2101 aattgtcgtt tcaatggatc tcaatctgag aacaaagatg atttcagtcc tagaagccaa
     2161 gtatgaattt tctaagtttg cacgttttac tgactattgc ttcggtattg atcctctcat
     2221 gattgatgac caaaatctag agatgtttcc aagagacagt gctagcctag aagaggtgga
     2281 aaatcgcatg cgacttttaa aagaagccta tggtttcaag gccattttcc ataccctccg
     2341 ctctagtgat gagcaagaca aaaatgtcta tcaagcctat gctctagaag aactatttga
     2401 agagtctgtc caactaaaaa ctgcagtcta tcaacgaatt ggtagcgggg atgcctttat
     2461 atctggtgcc ctttaccaac tactccatca ttcctcccta aaaactacca ttgactttgc
     2521 agttgcgagc gcaactctca aatgcactct tccaggagac catctctcca cttcctcaac
     2581 tagtattgaa aatttactgg caaatgcaca agatatcatt cgttaggaga attacatgac
     2641 caaatcagat acgattattg aactaaaaaa acaaaaaatt gtcgctgtta ttcgaggaaa
     2701 tacaaaggaa gaaggactac aagcctcgat tgcttgtatc aagggcggta tcaaagctat
     2761 tgaaatcgcc tataccaatc agtatgcagg acaaatcatc aaggaacttg tagacttgta
     2821 tcaggacgat cagagtgttt gtatcggtgc aggtactgtg cttgatgccg taactgctag
     2881 agatgccatt ctagctggag caaattacgt tgtttctcca tctttccatg ctgaaactgc
     2941 gaaaatgtgc aatctctaca gcacaccgta cattccaggc tgtattaccc tcacagagat
     3001 cacgactgca cttgaagccg gtagtgaaat catcaaactc ttcccaggta gtactctcag
     3061 tccagcatat atctctgcag tcaaggcacc gatcccacaa gtttccgtaa tggtaaccgg
     3121 aggagtcggc ctaaacaaca tccctcaatg gttcgctgct ggtgcagatg ccgttggaat
     3181 tggtggcgaa ctcaataaac tcgcttccca aggcaacttt gaccgcatca gcgagattgc
     3241 ccaacagtat attacactca gataaaatca taactacccg tctaacgggt ggtttatctc
     3301 agagctataa gcccaaatca tcagccagcg cctaaagacg ctggctttca cgttgttcaa
     3361 gccttattgc tcttgactcg tcacttgcct ctttaagaga ctttggtatt acttaccact
     3421 atccctaaag ggatcctcat attcttttac actcaattta tctagtgcta tagtagattg
     3481 aaactggaat agtacacctc tgcttctaaa acattgttaa aaatcgattt gactgtcctg
     3541 atcgattttg tcctgttctt atttcatttt actatatatc atactttact cgttctcaaa
     3601 ttttcatact catgaagaaa tcatccactc gataatttct ttaatcttga ctatatttct
     3661 taattgtggc ttcattaagc cctactggac ttacataata accttcctcc cagaaatgcc
     3721 gattcccaaa cttgtacttg agattggcgt gtttgtcaaa catcatgagt gcacttttgc
     3781 cttttaaata ccccataaaa cttgaaacac ttagcctcga cggaatactg actaacatgt
     3841 gtacatggtc tggcattaag tgaccctcga tcatttcaac acctttataa ctacacaagc
     3901 gatgaaatat ttcgtctaaa ctacttctat attgattata gatgactttt cgtctatact
     3961 taggggtgaa cacaatatga tagaacacct ccactttgtg tatgataaac tatgagtctt
     4021 ttgtgccata ttttttctcc tttcgcttta caattggatt gaacaccttt attgtatcgc
     4081 gtttggagtt tttttggtat aaccttcgac gcgcacccgt atagcgggtg gttgttttgt
     4141 ctcgcacctc acggagcgag acggactaat atagtggagt gaaataggat acgaacaaat
     4201 tgattaggaa aatcaaatga atttatagaa atcttttagc agttataacg ttctattcta
     4261 gtttcaaaac gctatagtca cataataatg aagtaaaaaa ggataagtat caacttatcc
     4321 ttttttaaaa gaaaaatccg aagatatttg gccttcttcg gattttttct attttccaca
     4381 gtttcatgta attcatctag atgatgaaca aattagttgt tctttcctct acggaataga
     4441 taaaatgccc caagtagcaa gaaccctaga cttgccaaga ttgactgacc ttctcctgtc
     4501 tgagggagat tcttttgatc cgaatggttc ttttcctctt cagatttttc cttttctttt
     4561 gaattctgta cttgtggctg agctgcttgc tctagctttt taaagacttc ctgatctgga
     4621 gctgattcct gggtttcagg attatagtag gcaatcttat attcatcccc ttcttttcga
     4681 atggtataga ctccacgttt caaaacttgg aattggttgg aaatagtaga gacagaatca
     4741 tcatatttca caatgcccca aactccttgt ttagcatcat aaacagactg aagggtttcg
     4801 ttattttcga tgaggctact ttctaactct tttatcattt gattgaaggt ggcacgatcc
     4861 acgttaggaa tgagcatata gccataagaa tctctatttt gcttatgagc ctgactaatc
     4921 gtaagaaatt cattttcaac ttccttgtct gactgtcctt cattgatatc cttccaggct
     4981 cccttttgca aagccttact catactgatt gaactcttct taaagaaaaa gtaaccaata
     5041 ttctttttcg aatcgaacga ttctaaaaag acactttggg tttcaggata atccttttct
     5101 tgttctgtaa gggaggcttc tttatcattg acatagactt tatatggatt acctgattcc
     5161 agttttctct ggtcaattgt agttgcagca gtatctgttg aagtgttttg gatattgctt
     5221 cctaaaaagg cgatcttatc ctttagcata aaccagctct tatgagcagt caatgtttga
     5281 ttccagttgg tgaaatccat ggttgctgtc gcattggcat catctagttt gctcgttcca
     5341 acgaaagcag acggtaaaac tttacctgta tcgctatccg ctctcttagc atccgtctct
     5401 gttgtaccag gcatcttata tggattaact gttggccagt agccatcgct atagtgactc
     5461 aaatcgccat tgtaaagata gaacatccca tcactcgtat accaaccacg tttattttcc
     5521 ttgttcatgt gttcgtaatt caaggtacga ctggaaaaga gtgacaagcc aaatccaaac
     5581 cctttctctg cattgtacat ggctgtttta tccatcttgt taaaggcaga taggtaactt
     5641 ggtcttggaa cacttgcgac tcctgcatca cttaacaagg attgcatcaa actgatatcc
     5701 ttataagtct tcaaattctt aaagacatca taataactat ccgattgaac aatggtcttc
     5761 acaagactct gcaaacattg tttggtttct ccttcagaca tatccgctat tcggtgaatc
     5821 cctcttagta cttctactgc ggccacgtgc ccctcgctat ttgcacgact gatcgagcgt
     5881 ccacgactca tatccatcaa ctctccattc accagcaaag gagcaaacga tttatcaatc
     5941 cagtggtaca tggtttgcat tttatcttta tcgattggat tcttggtctt ttgaatgact
     6001 ggcaacagtt gagacaggcc atcaatcaaa acattcccat aagcacccgt ataggcaaca
     6061 ttggtgtggt cgatatagga tccatcttga taaaaacctt caccttggtc taccaacttg
     6121 aacacttgct caatcgagcg aatggtagaa gaaatttctt gatcatcctt acgcagtaaa
     6181 ccagctatta cttttaccct tcccatatca actaagtttc cacctagagc cttgaatggg
     6241 ttatcagtcg tctttcggaa atgttcggga tctggtacaa atttttcaat cacatctgta
     6301 tattttttaa tttcctcatc agagaagtat tctttcatca gagacaaggt attgttgatg
     6361 gcacgaggtg taccgatttc ataatcccac cagttcccaa caatgctctt ttcactattg
     6421 tagacatgtt tatgcatcca ttccatggaa tccctgactg ttcgaacgac agtttcatct
     6481 tgataataac gagaagaagg attggtcact tgcttggcca tctcctccaa tttccgataa
     6541 gtggcagtca gatttgcaga cgttttataa tttgaaaatt tttcccacaa ataggtgcgg
     6601 tccgcctgac ttgaaatact ggataggcta tcagctacct ttccttccaa ttcctggttt
     6661 aatttggcca tctgttcatt tttagaatca tagtattgat tcccagcgat gatgccattc
     6721 cagtcatcca aacggtctgt gtatgcatcc ttaacagagg ccagaatctt caaaggaatc
     6781 tttttcactt ccttgccatc tttactgaca atgacattgg ttgtcccttc cttaagaggt
     6841 tctaaaattc catttttgac tgaagcaacg tcaggatttt ctaccttata agtatagtcc
     6901 gcaagagaaa aaacatgttt ttttccaatt ggtaaatcaa tcttttcctc aagctgttta
     6961 tctgtttgag aatcctcaga aagctggtct gctacctcta ccagctcaat atccttaaag
     7021 gaaacagtcc cagttcctgt ttcatagaat aactccagct tgattttatc aacatctaaa
     7081 gtcgggctat agtctgcttc aatggtctgc cagtcctttg ttcctgacgt cgttgcagaa
     7141 ttccacaatc gcttgtcctt accactttcc tcaatgatac gaactttggc aatcccgatt
     7201 ttattatctg ttttaatctt gaaacgcagt ttatactttt tcttagcttc aataggaacc
     7261 atacggtgaa gcgctgccct taatttctca tggcttgaga tagtgatagc cccatcctta
     7321 gcctcaatga ctcgagttga ggcatctgca ctattcttct ggtctaccca agctgaccac
     7381 ccctgagctt tgcttcctgt ccacggactg cttccgtcct ccgtctgacc aaaatcacca
     7441 ttttcaacta aattttttgt atccctttgc tgatactgaa cttccttctg gctttgctga
     7501 atggtattcg tagtcgtttc ttccgctcca attgtcatat aatggtttaa taaaaatcct
     7561 gataaaacaa gtgaagacaa actcacaatg agcttctttg tttttgtttg catgtataca
     7621 ctccaataaa catattgtaa aatcaatagg aactaaatct caaattagag atttatagca
     7681 actaagtaaa ccggtttaat taaatcatac catttttaaa gttatttgta aacggtttct
     7741 atcatattga aggcttattc ttcaacaata tcttccatcc gactcgacga aaaaagtaaa
     7801 aactgttaca atgtagaaaa agaaaagagg ccctcatgac ttcactttat gatttttccg
     7861 ttttgaacca aaacaaccaa gcaactccct tggatagcta tcgtggcaaa gttctcttga
     7921 ttgtcaacac tgctactgga tgtggtttaa cgccccagta ccagggactt caagaactct
     7981 atgaacgcta tcaagatcag ggcttcgaaa tattggattt cccttgcaat cagtttatgg
     8041 gacaagcacc cggcagcgca gaggaaatca acgccttctg tagcctacat tttcaaacca
     8101 ccttcccacg ttttgccaag attaaggtca acggtaagga agcagaccct ctctatgtct
     8161 ggttacaaga ccagaaatcc ggcccactag gaaaacgagt cgaatggaat ttcgctaagt
     8221 ttctcatcgg tcgagatggg caagtctttg aacgcttttc ttcaaaaaca gacccaaaac
     8281 aaattgaaga ggcgatacaa actctactat aattcacaat ctcactatga ttaggtttcc
     8341 tttaacctga tgaatagtga gattttttga tgggctttga cttaaataga aaaacacccc
     8401 atgatatgaa acatgaagtg ttgtaaagtc tatgttgtag gtgcttattt cacaatttca
     8461 atgtgaccag tgataacgaa taccatacag aatcttcata tacactaaac aaatgacttt
     8521 ctaattattt caattagttt tggctagtaa atatcatttc caacaaacgc cctctcaatt
     8581 ccttatcctg atgatgcaag atattcatta agtcatgaga gtttttcgca ttgatgaatt
     8641 gatttaacaa tctatctttt aattcatatg gaagagaagc tgtctttagt agtctaaaaa
     8701 cttcgtcatt taaagatgtc cttttattat ctttccattc aaatttagct gtatcattct
     8761 tatttggcaa ttcaattata gacacattcg ttcctttaaa atgaattcta tgttttctat
     8821 tgcttggaac gatactagaa tctccttgta atgctaactc taccattccc atttcccaat
     8881 cgattgataa tcttgtttta tatctttgac cattttgatc ttcaagcatt tcaaaagaat
     8941 gttgttttcc tgggaataca taccaatcta caacttcagg taaatcaaca cccataccta
     9001 tctcagaacc aaccaaggga atgattgcac cactttttgc aaacacaggc gtagtcgaga
     9061 tgtccctata aacacttaac ttcacaccac ctgtgtattt tttctctgaa aagaagtcat
     9121 accattcacc ttcagggaac catacatcta cttttgcaga ttggaatgtc aaatccatct
     9181 tttctacaat gggagccacc atcagttctg ttccaaaaaa gtattggttt ggaacattat
     9241 agctctcatc attctctgga tagaaataat agattggact gattaatggg gcaccttcct
     9301 catgtgtctg tacattcatg gtatatagat agggaatcat ctgatgtctc aaacgaaggt
     9361 atttcttcat aatcttagat gttgtttctg aaaaaaacca aggttcttta ctattaaaag
     9421 gacttctaga actatgtaat cgagtaatcg gactaaaaac accaaactgt agccatctag
     9481 tttgtagctc ttcgtcataa tcccccaaca tatgtccacc gatatcatga ctccaccaac
     9541 tataaccgat attagatgct gtcgctgtaa aatagggttg aaatcttaag gaattccaac
     9601 taataatagt atcccctgaa aaaccaacag ggtagcggtg actaccagga cctgcatatc
     9661 ttgataaaat caaaccacct tctgcatttt tacaactatc ctgatagtga taatggttta
     9721 aaagccaaag tggatctagc ataccttgtg tcccttgttg ccagtcaatc caccaaaaat
     9781 ctactccctg cttttctagt tcataatgaa catctttaaa gtaggcttcc ctaaaagagg
     9841 gattaaaaaa atcaaaaata gcaggttctt ctagttctac atttaacccc aaccgttttg
     9901 cgatttgagg ataagcttct tcataagccc gtatcccatc agcaggatgg acatttaagg
     9961 agagttttag ctttctatca tgaagttgtt gcaataactg ttctggattt ggtattaagt
    10021 ttctattcca actatatcct gtccagccac ttccaaagcg agctggaatg tcagttatat
    10081 gccaatccat atctaacaca ccgatagata atggaatttt ctctgtttca aatctgtcta
    10141 ttaaatccaa gtattcatcc gacgtataag gccaatatct actccaccaa ttgcctaaag
    10201 catatcttgg caacaagggt gttgaaccag tcaaatggta aaaatctctg attgctcctc
    10261 tataatcatg cccataggca aagaaataca ggtcaatttg attttctctc tcaatataac
    10321 cagattgttc atcccaaata aatccttgag aatcatccaa taaggctata ccatttcggc
    10381 taataattcc atcttctaac gagattgctc catctgcctt atccagagtc cgagctgttc
    10441 cttttaacgt ttcaatagat tcaccaaaat accagcgact accatatacg gcaaaatttc
    10501 cttttaattc tataaataaa ttttcggcgt taaattctcc tttattaaag tgcagatgaa
    10561 aatagtccgt cataatatct agtacgtttg atgtctcgat ataatctaac gaaatttggc
    10621 caaaatctct attatagata agttgtgtcg ttctatcctc aaaacttcca gtttgagagt
    10681 attctaacct tactagcttg tctgttaata cagagattcg ataaaactct cccttaaaaa
    10741 ttttcaattt gttttcctcc ttttatggta gcataaaaac agaacgcacc atttttgatg
    10801 cgtttttcat tattctgaat gcaatgttct atctgttata tctatgacaa ataatagtca
    10861 attgaaaaaa tgcagtggac aaaatatctt ttaacaaacc aagagtttat taaagagtta
    10921 tcacttttca acttttctaa gcttatgcag ttgtgaaaca aactactttt aaactattaa
    10981 ctaagatagg attgataaat aatttcaaac tcttactagc aatcatacga tattcaagct
    11041 cacgtgcttt tttccttcct gcttatttct tagaactgaa gaacccggat cggtatataa
    11101 attatccgga tcaacatagt cataagattc ataacagttg cgcttcatta agtcatcccc
    11161 agagcaagag cttcatctcg taatttttca acatcactaa ccgtaggtcg ccatccttca
    11221 atcatatttg tacttaaagc ataccaaaca ctcttaaaaa cggatcggtt ttcaaaagct
    11281 attcccatga ttgtcatctt ttctttatct atatctaagg acatatgcta cctcctttag
    11341 atacattata ccatgtttct ctgtagcttt taaaaatttt attttgtttg tcatatctaa
    11401 gttttcagca cgcttatcct attttataag cctcaaaccc aaatataaaa cgcattcttt
    11461 ttgctttttt actattgtat cgtattctac gataacatac tttactttat tgttttttta
    11521 aataacagca gttccctgtt tatcaactat tcgaactact ttctattttg cttcataccc
    11581 tacatagcga aaaaatatga aaaagcagag aagaatatct taaaaagacc tcttcactgc
    11641 taatattaac actcattatt taaactatat ggattctatc atcgagtata cttttttact
    11701 tattagatac cttgctcttc tttcaccaat ttttgatcat atacacggat gaatggaaga
    11761 tagactagga atgctgcaaa tgcacatact agagcaacta atacagctcg aagatctgct
    11821 gtccctaaga aagctccaat ccctactgga gttggccatg gaacctgtgc gataattggc
    11881 ttaataaagt ttagagaatt cgctacgtaa taaatagtag cagtaaccat tggtgctaaa
    11941 ataaatggta tagccaaggc tggattatag ataataggta atccaaaaat taatggttca
    12001 ttaatattaa ataaggctgg aactacagat gctcgtccta ttgctttaag ctgttcagat
    12061 ttagaggcaa aagcaatata taaacatagt cctaaagttg caccagaacc acctgcaatt
    12121 acaaacatat tagaaaattc acctgcaaca gcgaagtgcc cgccagcagc attttcagcc
    12181 atgttagcaa gagcaattgg actaacaaat gcaaaaacaa tgttcgcacc gtggatacct
    12241 acaatccaaa gtagttgagt caatagataa ataatcatta aaccaatcca cgaattagtc
    12301 agattggata caaaaccaaa tggaattgca atgactttaa aaatatctgt tcccattgct
    12361 acaagaagac cgttgataaa gataacaaca aatgcaacaa caaatcccgg aaccaaagcg
    12421 gtaaatccac gagaaactcc ttctggaaca gcttcaggca ttttaataac ccaattatgt
    12481 ttaacacaca tacgataaat aagaacagtc acaattgcca taatgattgc ggtaaaaatc
    12541 cctgttgtcc caaaacgtgc gactacattt cccattgccc atccatctgc aattactgca
    12601 ccttctttta gacttgtcac agtcttcatc attccaccat caaaaatgat ttgcggtact
    12661 gtcatgacaa aagccatcaa ggcaagcaag gcaccattaa gaggattcat attgagttct
    12721 tcttcctctg cataaatttt tgtcaattca tatgcaagtg atagaacgaa ataaagagat
    12781 agagaaccca tagtcgcata gtttgcaacc atgtaaagtg atgtgaattt atcaaatgaa
    12841 gcagagaaaa tatctgccac aattggccaa aatgagaaag cttgtggcaa aatactgaat
    12901 accaaaaaca ttgatcctac aatagtaaat ggtacagcag ccatacctgc agccgtgata
    12961 gcacgtacta ctttaaactg agcaagtttg cccattggtc ccataacatg gttttcaaga
    13021 aaaccaaaca acccgttttg ttgatccata aatagacctc cttaataaaa cataataatt
    13081 tttactttct aaagactagt ttcaaataca aattatacta gatcaggatt ataaactaag
    13141 tgagttcttt tccaattgga caaattgttg ataagcctta tctgttcgtt tataaatttt
    13201 tttaattctt ctaatgtcta acaaactcag aactaaacct aatagaagaa ctacaaaaac
    13261 aaataaacgt gctacttggt tattttcaaa aatcggaaaa agattcttaa accaacttgt
    13321 ccaagttaaa acaagtaatc ctattgaaat aagcatttgt attctaacaa acattagtgt
    13381 tattcccaac ttttctttcc tatttccata aagtttaaat tgttcaacag ttgctaaaat
    13441 agaaaatact atgagcataa tggggaaaat aataataggc gagggactaa taaactgact
    13501 caaaagccaa taaatattcc caaaaaagaa gagtgctatt gaataacgta gaagaagata
    13561 tcgattgaaa aaagtattag ttagagccat ctctcgacgt tgttgttcaa tcttttgtcg
    13621 ttctttttta tccatatcat ttcctcctta tataacaaca catatttagt taactttctt
    13681 ataaagagct aacatttcct ttgctacttc taataatgtc atagtggtca ttaaatgatc
    13741 ttgagcatgt accatgataa tttcaatttt aatttccact ccacttgcgt attcttgcaa
    13801 gagtttggtt tgtgcatgat gcgcttcaag aattatctca tttgattgat ttaatttact
    13861 ttctgcatca tcaaaactac cttctctcat ttttgcaaat gcttcatgta tttctgacct
    13921 tgcatttccc gaatgcagga taatttcaaa tgctgcaacc tgcagttcct cttgattcat
    13981 ataaacctcc tattttatct tctcaaatat gttaataaaa tcttcaaagt tattgcaaga
    14041 tattagctga ttttgcaatt catcattctc tgtcagagag actatctttt tagtcacagt
    14101 tgccaaacct tcgttcccat atattgatgg agatagaaga aatactagct ggacatgtga
    14161 actttgatta tcccagagta acgaatcttt acaaattgca accgaaacct ttccctctgt
    14221 accaaagggc tgaataggat gcggaactgc aattttttca gaaaaaacaa ctgaacttaa
    14281 ttcttcgcgc tgtttaattc cataaagtaa agattgttca aactcatttg attcaccaac
    14341 agataaactc tcaaccatct tttcaagtaa atttaccttg tctgattcag tacatattaa
    14401 aaagttttct ttactaaaat actgtctaaa gccgttgttt tcaaatttgt taatctttga
    14461 tgattgtaca taactagaaa cttgcatcta atccatagct tttctaatca tttccatctc
    14521 atcactctta agaaacacac taactttaaa aactgggatt tgaaaatata gatttgataa
    14581 atcaatagct gacactataa aatctattcc tttaagtttt tcttgattca attcatagta
    14641 gcctattaca tcaacaactt ctactcgctt cccaaactcc gtttccaaac gatttcttaa
    14701 catttgggct gcaccaaatc ctgttgcaca aatagcaaga atattaaact tagtactctc
    14761 tttgctacgt tccatagcag ctaaaaagtg aagacttaca tatgctactt catcatctga
    14821 tattgtccac tccaagaact tgtccatatt tgcaagaatt tctctagtca taaagaatat
    14881 atcactataa ttctgtttaa tttcatctac caaagggtta tttaaggtaa tccggctttc
    14941 taaacgtact tgtagtgtca ttagatgagt tatcaatcct tcaattagtt ggaaatctga
    15001 agaaaagtta tacatatcat ctaatcctaa attctgaaat gttttaaata aagatttttt
    15061 taaaacttct tcagaaatat tcttctgatt tttttgacat tgttgactct tagctaacaa
    15121 atgcaaagta atgtagtcta tttcctgaac tggaaattcc tgatttgtta cttctcttac
    15181 tttagaaaga attctttggg caacctttct ctctattgca tcatcagtca tctgacagtc
    15241 tatatttttt atttcaaatc cggattttaa acgaatcaca gacaatgcta tgtgaactac
    15301 taaattctgt agtacaaaat cagatagttt taggttggcc tcttggcatt catccaaaac
    15361 aattctagca aattcttcta atggaacagt ttgatcaaaa aagttaaatt ttacatagca
    15421 atgtattgtt ttaaaaaatt gattctctag gaaataattt atgataaaac gtcgtttatc
    15481 acgttcctcg cctgagacat aaactccttt attcgcccta ctctcaatgg acaaattata
    15541 ctctgataac atcactcgta tctttctgaa atcatgagat aatgttgaac gactaacgta
    15601 aagttcatca gctaaatcat caaaaagaac tggaacttgc tcaaataata atttatttaa
    15661 gataaatact aaacgatcat caccttttga aaccgcagtt ttcgtatagt cttcttccag
    15721 ttcataagtt tgtctaaact cctggtaagc gccttgattc tcaaaaaata tttgataccc
    15781 ttgaccttgt tttgaaatca accggactcc ttgaataatc attgtcttct caattaattt
    15841 cagtacatta cggacagttc tatctgaaca ggataaatat tctgccagtt ctttgcttgt
    15901 aacaaaacgt tccttatttt ttattaaaaa ttgaaggata tctttctctt taatgtttaa
    15961 cacattcatt ccctcctaaa acgtatgttt tcatatattg aagcatatta tacacttaaa
    16021 tcagtttata tcaaactcaa aacaatttat cttaacctaa atatttattg acatttcatg
    16081 tgttcatcaa atattctcaa gaatcaaatt agccattttt tcaattccca ttggaatagg
    16141 aatataggct tgaggaggta tttgtacaac tggttttcct gctttagaac cagcctcttc
    16201 aaattgctta aagtacattt ttgtttgagg actgacaaga tacaaatcaa aagctgctgc
    16261 tgcgatagct ttccctcctt cagtagcact aatagcatca actacaatat ctttcccttt
    16321 tccttttaga aactctgttg ttttctgtgc cataagtgat gaagacattc ctgctgcaca
    16381 aataattaaa gcttttgcca taatattttc tccttttctt aaatccaatc aaagctgtgc
    16441 taagttggct tatttgttat ctatttttat tataaaataa agcgcttcca atgacaattc
    16501 cctcattttc ctaaatgata tggaaaaaaa ttatttatac ttcaatttat aaaataaaat
    16561 tattcctgag agtagaaatg aaacactatt tgctaaaatc aaaggcaagt ctcctatacg
    16621 aataccatga gcaagccaca atgcaatacc aataacttgc ataacataca tacctagagc
    16681 aatagatcct gtgtcctttg tcttaactac acgaaaaact tgtggtaaaa atgcaaatgt
    16741 tgttaaaatt gctgcaatac ttccaatcat atgtcacctc aatatgctaa acaaactgag
    16801 aataatctca gtttgtttat actattctac tgattcaccg ttagatgaaa taacttcctt
    16861 ataccagcca aaagattttt tcggggaacg attataactt cccttcccat tatcatcttt
    16921 atctacataa ataaagccat aacgtttccg catttcaccg gtaccagctg aaaccaaatc
    16981 aatacatccc catggagtat aacccattaa atcaacacca tcttcaacta cagccttttt
    17041 catttcacga atatgggcac ctagatattc aattctataa tcatcatgta ccataccatc
    17101 tgctgcaact tgatctatag ctccaaaacc attttcaaca ataaagagtg gtaagtgata
    17161 gtggtctgta aaccaattta acgcataacg caaaccttct ggatcaattt gccactccca
    17221 ttcagaagcc ttaacataat tatttttcac taaatcttct gtttcaagat aatcaaaata
    17281 aggattattt tcacgatgag agtcgatagc aaaggacata tagtaactga aaccaatgta
    17341 atctacagtc ccaccaagta aatcttcttt atcctgggca gtaaaatcaa ctgaaatacc
    17401 ttttcgttcc caatacttga aaatatgctc aggatattta cctaaaacat gcacatcagc
    17461 aaaataataa cgcttctgca tagctttcat tgccattaag atatccttag gattgcaagt
    17521 aactggataa attggacaca tcgcaatcat acaacctatt tgaaaatctg gattaatctc
    17581 atgaccaatt tttacagctc gtgcagaagc aactaattcg taatgtgctg cttgatacat
    17641 aattgcttct ctattatcac cttcctcata tacaatacct gagttagtaa atggtgcaaa
    17701 atcttcctga taattcgctt gattattgat ttcattgaaa gtcatccaat atttaacctt
    17761 atctttgtaa cgtttaaata cgacttctgc aaaacgagca aagaaatcaa tcaatttcct
    17821 atttttccaa ccaccatatt cggtcactaa gtgataaggc atttcaaaat gagatagagt
    17881 gatgacaggt tcaataccat tctttaagca ttcatcaaaa agattatcat aaaactgtaa
    17941 tccttcttca ttcggctcta actcatcacc ttttggaaag atacgtgtcc atgcaataga
    18001 ggtacggaag cacttgaatc ccatttcagc aaaaagtgct atatcttctt tataacggtg
    18061 ataaaaatct atcgcctcat gatttggata atatttaccc tctaaaactc ccaaagtaat
    18121 ttcacgagct actccatgac gaccagcagt cataacatca gcaacactaa ttcccttgcc
    18181 accttcttgc catccacctt caagttgatg agcagcaaca gcaccacccc ataaaaatcc
    18241 atctttaaaa gtagtcatct tttttcctcc tgactttgat actcttatta taaaccttaa
    18301 accaaaagat gaaaacgcat tctttttcct tattgttaag gaaagaagta atttttaatg
    18361 gaaatagaac aatatcttct tgtattctcg taatgatatc tttacgattt tcaatacttt
    18421 caaactacaa aaactctcac aataattcta attccctgtg tctataaacg acttatcgct
    18481 ttctggcatc ccagaatcat cttctatata acgttcaact tgcatctgca agtgatattt
    18541 ttttcttaaa tctaagattt tctgcattgt ctttgattga taatgtttat ctaaagtttc
    18601 ttgatttatc cactgatcaa taaggagaat agttccctct ttttcaattg gtaaaaaata
    18661 ttcgtatttc aagttacctt tttgatttct aatttcttta acaaggccac tatcaagcat
    18721 ttctcttgca aactttattg cactatctcc atcaccttta taatatacat gaatagtcaa
    18781 tgtcatctta tatcctccaa aatcatcctt caattttaaa aaaacaagtt tagatgagga
    18841 tctaaacttg ttttttatga actaattatc taacgtttcg ccattacttt caatcacttc
    18901 tttataccaa taaaatgatt ttttcttata gcgatttata gtcaattgaa acaagagca
//
LOCUS       DAVKNL010000039        18808 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVKNL010000039 DAVKNL010000000
VERSION     DAVKNL010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18808
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.039"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(<1..400)
                     /locus_tag="VW411_001385"
     CDS             complement(<1..400)
                     /locus_tag="VW411_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794104.1"
                     /translation="MTAEIGILNKNGVVLASDSAVTLSDGKNSKVFNSARKLFTLSKE
                     HSVGIMIYGNASFMEIPWEVILNEFKQAIGTDLLDNTAQYVEKLIEFLISFKHLQVED
                     LLRNYIVRSTRSILDSIAYEAQETADLRVSN"
     gene            complement(418..711)
                     /locus_tag="VW411_001386"
     CDS             complement(418..711)
                     /locus_tag="VW411_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9794105.1"
                     /translation="MKNNAIGSNWKDVRAELFSKEEILESDMRVAIMSELIEARNEKG
                     ISQKKLEEMSGVSQPVIARMETGKTSPQLDTVLKVLASLGKTLAVVPLEHEQV"
     gene            complement(701..1066)
                     /locus_tag="VW411_001387"
     CDS             complement(701..1066)
                     /locus_tag="VW411_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU9794106.1"
                     /translation="MHNIYFYKDKNGNEPVFDYMRELTSKKGKDSRIKLNKINDYIEL
                     LSQHGTRAGEPYIKHLDAEIWELRPLRDRILFVAWMDGSFVLLHHFMKRTQKTPKREI
                     EQAKRELADLKERGLDNEK"
     gene            complement(1263..1673)
                     /locus_tag="VW411_001388"
     CDS             complement(1263..1673)
                     /locus_tag="VW411_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794107.1"
                     /translation="MTKKKIERISVIHREKILWLKWYFMRDKEQPKYSVLERKMFDAA
                     KNQDMLAYQKYATIKQITDIRVQTSEADILEAVKEVYVYNHMNVIGACQRILFISQSP
                     AYDKLNKWFNIYSDLYFSVVPLPKMGVYHEMVGI"
     gene            complement(1691..2002)
                     /locus_tag="VW411_001389"
     CDS             complement(1691..2002)
                     /locus_tag="VW411_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794108.1"
                     /translation="MPEFIIVEGNNDLGEFFQIDGELFSDNELLENLKKWREWEVPVI
                     IDDWCNRILNEDETEILYFPTHEDKMNYIRVEKDLEPLYHTSNKIYATISKSEWLELL
                     N"
     gene            complement(2010..2438)
                     /locus_tag="VW411_001390"
     CDS             complement(2010..2438)
                     /locus_tag="VW411_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEU9794109.1"
                     /translation="MAGNENDNLTSKQIKFIDAMLTEPTIDKACQKAGVSRATGHKYL
                     KVAAVKKTLRIKQDEMMDKTTQMLYLASSNAVSVLNDIMMDSKVNPFIRTQAAKAILE
                     QSYKTHEIFGVVRQIEELRLEIEEVSKGNQRVTRTQGVIK"
     gene            complement(2485..3135)
                     /locus_tag="VW411_001391"
     CDS             complement(2485..3135)
                     /locus_tag="VW411_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794110.1"
                     /translation="MTENKDDKLLKMMEKGFVLYSKNGIIKYIEIPEHGSIKLKAQDG
                     QLVYKEVIIGKVYAEQELLKYKKEGENYSQNTAQLLYQQLEKEKNAELANHESKEEPI
                     TADDAAELSLLSSIKLTVAEMREYLEKYKNKPLALRKLEDIMDNDTTLAYIEIDMEQF
                     SQQQRLEKLVHFLDRKIDYFHGGLLINGDKIDLVQHEMIVEGNSEAMDAELQNYLA"
     gene            complement(3384..4268)
                     /locus_tag="VW411_001392"
     CDS             complement(3384..4268)
                     /locus_tag="VW411_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9794111.1"
                     /translation="MKSLQSSLGNIETFKLDELCPKHEIQLTQIKGERHVVVGWNENG
                     EAIKEVRCIPPYCEQCQEEQKKQDEEDAIADNLNAKLYMQTYNVLMSNKSYVADEFKT
                     KTFDDFNAVTSEEKRFLEFAKGQVQKYLDGMRGNTLITGGTGIGKTLLSVAIAKGINE
                     GYKTKGEPKSVLFVSLTEMIKQIKEGWNYGKGATLTEFEATELMKSVDYLIIDDLGAK
                     NAVIKPKSDWEQDLLFDVLNNRENTIFNTNLDSDELKIVYNERNYSRILKGLEGNTFK
                     AFGIKYKRYSLNQLKSNM"
     gene            complement(4280..4726)
                     /locus_tag="VW411_001393"
     CDS             complement(4280..4726)
                     /locus_tag="VW411_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEU9794112.1"
                     /translation="MTLDLDNMTQAEFDEVMAEIQLQSPNIFQLISDFVNKKVTSVEI
                     DDLLNMKRAETGKALSPLQIQELQYMLEDFSPELIHEALKEAVSQGKANFAYIKVILN
                     RWKQDNLLTVELVRNSRAAREAKKQQTNQLEPTSYEDWIPTQEKPF"
     gene            complement(4723..5175)
                     /locus_tag="VW411_001394"
     CDS             complement(4723..5175)
                     /locus_tag="VW411_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794113.1"
                     /translation="MTETTYENLTRRIDRISAEMREISEENGLRILSLLESQTKSIKD
                     ELSRLLWIELPELNESHKIEAVSKSTTGMFFAPGIFEMDAMRKAFFKRQAKHFFENEA
                     EQQAYIEHAEKEYLEATVTLKDILFNSKNGTQKANKSCLIEKFEEAMQ"
     gene            complement(5210..5827)
                     /locus_tag="VW411_001395"
     CDS             complement(5210..5827)
                     /locus_tag="VW411_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HEU9794114.1"
                     /translation="MELVYMDGKKEPYTTSEIIAECAEVQHHTITRLIRENKADFEEL
                     GILGFKIHKLDTRGQPKKSYILNEQQATFLITYLKNTETVRQFKLNLVKAFFEMREEL
                     SEIRLQRALEKPKRKTLHDSIETWPNAPKHAHSTMNNLLLKAVTDMNAKQLREERGGY
                     NGIDSLTSEELEQYQAFEDMAIAMIELKMSYQEIKTMMFRSKKIS"
     gene            complement(6032..6184)
                     /locus_tag="VW411_001396"
     CDS             complement(6032..6184)
                     /locus_tag="VW411_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794115.1"
                     /translation="MKIVTFKPTKQIDDGFYLPGIDILFVSDKADAKDKEDVILFLSR
                     NGLNKS"
     gene            complement(6195..6461)
                     /locus_tag="VW411_001397"
     CDS             complement(6195..6461)
                     /locus_tag="VW411_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794116.1"
                     /translation="MEILSKEIQLQGLQLLKQTLETLVELEKQRSSKLDLISRKELMD
                     LLGISATTLDNWEDLGLKRYQTPMDGAKKVFYRPSDVYLFLAIK"
     gene            complement(6561..6914)
                     /locus_tag="VW411_001398"
     CDS             complement(6561..6914)
                     /locus_tag="VW411_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9794117.1"
                     /translation="MSKENKMRGYRNMLGLTQEKLGKKLGISKQSYYNKESGKTQFSD
                     KEKLKIKNLLIPLFPDITIEDIFFKQKYAKVKSAKNGIKAKIKAVYRRPNQRTRLRKN
                     NKTQAKARRVVLLGI"
     gene            7034..7882
                     /locus_tag="VW411_001399"
     CDS             7034..7882
                     /locus_tag="VW411_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9794118.1"
                     /translation="MKINNKLVGERIQNIRLSHGDSMEKFGEKFNTSKGTVNNWEKGR
                     NLPNKENLLKIASIGKMSVEELLYGDYNTYLHLKIMDLAPECIKNYDEYNSLHDDITN
                     KALQIAQNTISKIDYQISDETIKKFIDLAIEQSRDLQGNLLKNNLSNFDYSLFGGTRN
                     REYFYFGYRLTYKEKYLYTALLAILNESTKTIYIDDINTARFFNFFGNDIPNSSLKKI
                     DYIAPSEIVSFSTYVRQRSKVIPKILEHILKSSFLLENIDVSGYTVNILEDQLTKHRT
                     IKISKN"
     gene            7957..9120
                     /locus_tag="VW411_001400"
     CDS             7957..9120
                     /locus_tag="VW411_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU9794119.1"
                     /translation="MNIKEKIKKNGQRVYYASVYLGVDQLTGKKARTTVTATTKKGVK
                     VKARDAINTFAANGYTVKDKPTITTYNELVKVWWDSYKNTVKPNTRQSMEGLVRVHLL
                     PVFGDYKLSKLTTPILQQQVNKWADKANKGEKGAFANYSLLHNMNKRILKYGVAIQVI
                     QYNPANDVIVPRKQQKEKAAVKYLDNKELKQFLDYLDALDQSNYENLFDVVLYKTLLA
                     TGCRISEALALEWSDIDLESGVISINKTLNRYQEINSPKSSAGYRDIPIDKATLLLLK
                     QYKNRQQIQSWKLGRSETVVFSVFTEKYAYACNLRKRLNKHFDAAGVTNVSFHGFRHT
                     HTTMMLYAQVSPKDVQYRLGHSNLMITENTYWHTNQENAKKAVSNYETAINNL"
     gene            complement(9274..10578)
                     /gene="eno"
                     /locus_tag="VW411_001401"
     CDS             complement(9274..10578)
                     /gene="eno"
                     /locus_tag="VW411_001401"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEU9794120.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            10742..11188
                     /locus_tag="VW411_001402"
     CDS             10742..11188
                     /locus_tag="VW411_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEU9794121.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(11185..12300)
                     /locus_tag="VW411_001403"
     CDS             complement(11185..12300)
                     /locus_tag="VW411_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEU9794122.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     ASGQSLLNLASVSTENCYKIPEGVQIHILADVVSPLCGHQGATYTFGNQKGLHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGAGGGLAGGLCAFAQASIVSGIDTCLDLINFDKKV
                     SDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQAV
                     FSILEKSEPLEDSLKNASFYLERTAANIGHLLNMCKI"
     gene            complement(12309..12542)
                     /locus_tag="VW411_001404"
     CDS             complement(12309..12542)
                     /locus_tag="VW411_001404"
                     /inference="COORDINATES: protein motif:HMM:NF019887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEU9794123.1"
                     /translation="MISPQVKKETLEKWRKKLKLSKERTVAIGDGVNNLLMLKSAKLG
                     IAFCAKEVLKKEIPHHVDKRDFLEVLPLIDCLE"
     gene            complement(12583..12954)
                     /locus_tag="VW411_001405"
     CDS             complement(12583..12954)
                     /locus_tag="VW411_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEU9794124.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHCQLA"
     gene            complement(13013..14446)
                     /gene="glgA"
                     /locus_tag="VW411_001406"
     CDS             complement(13013..14446)
                     /gene="glgA"
                     /locus_tag="VW411_001406"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEU9794125.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(14443..15582)
                     /gene="glgD"
                     /locus_tag="VW411_001407"
     CDS             complement(14443..15582)
                     /gene="glgD"
                     /locus_tag="VW411_001407"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEU9794126.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEHGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(15572..16714)
                     /locus_tag="VW411_001408"
     CDS             complement(15572..16714)
                     /locus_tag="VW411_001408"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEU9794127.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(16704..18632)
                     /gene="glgB"
                     /locus_tag="VW411_001409"
     CDS             complement(16704..18632)
                     /gene="glgB"
                     /locus_tag="VW411_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEU9794128.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAIVTELPEKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSAIKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFASQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         5580 a         3975 c         3064 g         6189 t
ORIGIN      
        1 cattagaaac tcttaaatca gctgtttctt gcgcttcata ggcaatgctg tccaaaatac
       61 ttcttgtact tctgacaata taatttctta ataaatcctc aacttgtaga tgtttaaagg
      121 aaattaaaaa ttctattagc ttttcaacgt attgggcagt attatctaat aaatctgtgc
      181 caatagcctg cttaaactca tttaaaatta cctcccacgg aatttccata aacgaagcgt
      241 tcccatatat catgatcccc acggaatgtt cttttgataa agtgaataat tttcgggcgc
      301 tattaaaaac ttttgaattt ttcccgtctg ataaggttac agcgctatca gaagccaata
      361 caacaccatt tttatttaat attccaattt ctgctgtcaa aatatcacct aaactttcta
      421 aacctgctca tgctctaatg gtacaacagc taaggtctta ccaagacttg ccaacacttt
      481 taatactgta tcaagttgtg ggcttgtctt tcctgtttcc attctagcga taactggctg
      541 actaacaccg ctcatctcct ctagtttctt ctgactaata cccttttcat ttctagcctc
      601 gataagctca ctcatgatag ccacgcgcat atcactttcc aaaatttcct ctttgctgaa
      661 taattcagct cttacatctt tccagttact accaatagca ttatttttca ttgtctaaac
      721 ctctttcttt taaatctgca agttcacgtt tagcttgctc aatctctctt ttgggtgttt
      781 tctgtgtcct tttcataaaa tgatgcagta aaacaaaact accatccatc caagcaacaa
      841 ataaaattct atctctaagt ggtctcagct cccaaatttc agcatctaaa tgcttaatat
      901 atggttcgcc tgcgcgtgtt ccatgttggc ttaacaactc aatataatca ttaattttat
      961 taagcttaat tctgctatct ttcccttttt tactggtaag ctctcgcata taatcaaaaa
     1021 caggctcatt gccgttttta tccttgtaaa aatagatatt atgcactatt aacacctctt
     1081 cctaataaca attataacct aaaagttatt gtttgtaaat acttttaagt tattaaaata
     1141 aaaagcacct agtttcctag atgctagcac aatgacacgg attcgcaccg tggctacctc
     1201 tatcaaggtg tactccttct atactatccc ttgtgcttta gaatattata ccacacaatc
     1261 aactagatac ctaccatctc atgatatacc cccattttgg gcaagggtac aacgctaaaa
     1321 tacaaatcag aatagatatt aaaccactta tttaacttat cataagctgg tgattgactg
     1381 ataaataata tccgctgaca agctccgata acattcatgt gattgtacac ataaacctct
     1441 tttacagcct ctaaaatgtc agcctcactt gtttgtaccc taatatctgt tatctgcttg
     1501 atagttgcgt atttttgata agctagcata tcttgatttt tagcagcatc aaacatttta
     1561 cgctcaagga cactatactt aggttgttct ttatctcgca tgaaatacca cttgagccat
     1621 aaaatctttt ctcggtgtat tacagaaata cgctcaattt tcttctttgt cattgctacc
     1681 tcctaaatca tcaatttaac aattctaacc actcactttt agaaatagtt gcatagatct
     1741 tgttcgatgt atgatacaaa ggttctaaat ctttttccac cctaatatag ttcatcttat
     1801 cctcatgagt aggaaagtat agtatttccg tttcatcctc gtttaggata cgattgcacc
     1861 aatcatcaat aataactggc acttcccact cacgccattt tttaaggttt tctaaaagtt
     1921 cattatcact aaatagctcg ccatctattt ggaaaaattc ccctaagtca ttgtttcctt
     1981 caacaataat aaactctggc atatttctat tacttaataa ctccttgagt tcttgtaact
     2041 ctttgatttc ctttagatac ttcctcaatt tccaacctca attcttcaat ctgccttact
     2101 actccaaaaa tttcatgggt cttataagat tgttcaagta tagcctttgc tgcttgagtt
     2161 cttataaacg ggttgacctt actgtccatc ataatatcat tgagtacaga aacagcgtta
     2221 gatgatgcta aataaagcat ttgagttgtt ttatccatca tctcatcttg ctttatcctc
     2281 aatgtctttt taaccgctgc aacttttaga tacttatgac ctgttgcgcg tgatacccct
     2341 gctttttgac atgctttgtc tatcgttggc tcggtaagca tggcatctat gaatttaatt
     2401 tgcttggacg taaggttatc attttcattt cctgccatct attacctcct cattatcaaa
     2461 ataaagggtt gcccctttat ttccctatgc tagataattc tgcaattctg catccattgc
     2521 ctctgaattg ccctcaacaa tcatttcatg ctgtactaaa tcaatcttat ctccgttaat
     2581 aagtaaacca ccgtggaaat aatcaatttt tctatcaagg aaatgtacta gcttttcaag
     2641 gcgttgctgt tggctgaatt gctccatgtc aatttcgata taagcaaggg tagtatcatt
     2701 atccataata tcttctaatt ttctaagagc tagaggttta tttttatatt tttctaggta
     2761 ttctctcatt tctgccactg ttaatttgat actagataat aaacttagtt cagctgcatc
     2821 atctgctgta ataggctctt cttttgattc atggtttgct agttcagcat ttttctcttt
     2881 ttctagttgc tgatacaata gctgagcagt attttgggaa tagttttcgc cctctttttt
     2941 atattttaaa agttcttgct ctgcatacac tttcccgata atcacttcct tataaactaa
     3001 ttgcccatct tgagctttta gcttaatact cccatgctct ggaatttcaa tatacttaat
     3061 tataccattt tttgagtata aaacaaagcc tttctccatc atttttaata atttatcatc
     3121 cttgttttca gtcatgcttt tctcctttat ttcattttat tataatctga atacccctag
     3181 tctatttatt tcactaggtt tttagggttc gtatgctaaa atactaccct ttttgtgtac
     3241 cttatggctg acttttcaaa ttggttagtt tgccacactt ggtttatatg gtcgtggaaa
     3301 gcatggctat tacttctcaa agggcgattt ctcaccccat gaaaagtgtc tatttttgtt
     3361 taggtttgta agttaattca ttgtcacata ttactcttta actgattgag tgagtaccgc
     3421 ttatatttga tgccaaacgc cttaaaagtg ttaccctcaa gtccttttag aatacggcta
     3481 taattccgct cattgtaaac tatcttaagc tcatcactat ctaggttggt attaaaaatg
     3541 gtattttcac gattgtttag cacgtcaaag agtaaatcct gctcccagtc actcttaggc
     3601 ttaataacag cattttttgc tcctaaatca tcaataatta agtaatcaac agacttcatg
     3661 agttcagtag cttcaaactc tgtaagtgtt gcacctttac cataattcca cccctcttta
     3721 atttgtttga tcatttcggt taggcttaca aaaagcacac tcttaggttc tccttttgtc
     3781 ttatacccct catttatacc tttggcaata gcaactgata aaagtgtttt tccaatccct
     3841 gtacctcctg tgataagcgt atttcccctc atgccatcaa gatatttttg tacctgacct
     3901 tttgcaaatt ctaaaaatcg cttttcttct gatgttacag cattaaaatc atcaaaagtt
     3961 ttagttttaa actcatctgc tacatagctc ttattgctca tcaacacatt ataagtttgc
     4021 atatatagtt tagcattcaa attatcagca atcgcatctt cttcatcttg ctttttctgt
     4081 tcttcttggc attgttcaca atagggtggg atacagcgaa cttcttttat tgcctctccg
     4141 ttctcattcc accccactac tacatgtctt tctcctttga tttgtgttag ctgtatttca
     4201 tgcttaggac acaattcgtc tagtttaaat gtctcaatat ttcctaaact agattgtaat
     4261 gatttcattt tctgacctcc taaaatggtt tttcttgtgt tggtatccaa tcttcatagc
     4321 tggtaggctc tagttgattg gtttgctgtt ttttagcctc acgcgctgcc ctgctatttc
     4381 taacaagttc caccgtcaat aaattgtcct gtttccaacg gttaaggatt accttgatgt
     4441 atgcaaagtt tgctttaccc tgactgacag cctcttttaa cgcctcatgg ataagctctg
     4501 ggctaaaatc ttctagcata tactgcaatt cttgaatctg taacggtgac aatgctttac
     4561 ctgtctcagc tcgcttcata ttcaacaagt cgtctatttc cacactggtt acttttttat
     4621 ttacaaaatc agaaatcagt tgaaaaatgt ttggactttg tagctggatt tcagccatta
     4681 cctcatcaaa ttctgcttgt gtcatgttgt ctaaatctag tgtcattgca ttgcctcctc
     4741 aaacttctct ataagacaac ttttatttgc tttctgagtt ccatttttag agttaaaaag
     4801 aatatctttt aaggttacag tagcctctaa atactccttt tcagcatgct ctatatacgc
     4861 ctgttgctct gcttcgttct caaaaaagtg cttagcttgg cgtttaaaga atgcttttcg
     4921 catagcgtcc atttcaaaaa taccaggggc gaaaaacatt cccgtagtgc ttttagagac
     4981 cgcttcgatt ttatggcttt cattcaattc aggaagttca atccaaagta aacgggacaa
     5041 ctcatctttg atggattttg tctgactttc caataaagaa aggattctta ggccattttc
     5101 ttcgctaatt tctcgcattt ctgcgctaat tctgtctata cgtctagtta aattctcata
     5161 tgttgtttct gtcatgtttt tacctctgtt tctttgttgg tgtgattttt tagcttattt
     5221 ttttacttct aaacatcatt gtcttaattt cctgataact cattttcaat tcaatcatag
     5281 ctattgccat atcctcaaat gcctggtact gctccaactc ctcactagtc aagctatcga
     5341 taccgttata gcccccacgc tcttctctta actgcttagc gttcatgtct gttactgcct
     5401 ttagtagcaa gttgttcatg gtgctatgcg cgtgctttgg tgcattaggc catgtttcta
     5461 tactgtcatg caaggttttt cttttcggtt tttctagcgc cctctgcaga cgaatttcag
     5521 aaagttcctc acgcatttca aagaatgctt tgactaggtt tagtttgaat tgccgtactg
     5581 tttcggtatt ctttaaataa gtgatcagaa aagtagcctg ttgctcgttc agaatatagg
     5641 attttttagg ttgtcctcta gtatctaatt tatggatttt aaatccaagt attcccaact
     5701 cttcaaagtc agccttattt tctcttatta agcgcgtgat agtgtggtgt tgtacttcag
     5761 cacattcagc gatgatctcg cttgtggtgt acggctcttt cttaccgtcc atgtaaacta
     5821 gttccattac ggttctacct cctgtataaa tctggttagc ttacttttta attgcctcct
     5881 ctagcctctt ttttagcctc taaaacggct ttggctagtg gttaatatta tttaccactt
     5941 gtctctataa acgtgttaga ggcctttata acgacttgta tcgctgtatc gatatcctcc
     6001 gtggaatagt agatttattt tctaatatca ttcaagactt gtttaaccca tttcttgaaa
     6061 gaaataaaat tacatcttct ttatccttgg catctgcttt gtctgagaca aatagaatgt
     6121 caatacctgg cagataaaac ccatcgtcta tttgtttagt tggtttgaaa gtaacaattt
     6181 tcatttcata actcctattt tattgctaaa aataaataca catctgacgg acgatagaat
     6241 actttcttag ctccatccat cggagtctga tatcgtttaa gaccaagatc ctcccagtta
     6301 tcaagggttg tagcacttat acctagcaga tccattaatt ctttacgaga aattaaatct
     6361 aacttactag atcgttgttt ttctagctca actaaagttt caagagtctg tttaagaagt
     6421 tgtaagccct gtaactgtat ttctttagac agaatttcca tttatatcct ccatttttcc
     6481 tttttataaa ttaagttgtc attttcctct aattttgata aaataagagg agattattta
     6541 caaaggggtt tgtccccttg ctaaatacct aacaaaacca cgcgccttgc ctttgcttga
     6601 gttttgttat ttttccttag ccgtgtacgt tggtttggtc gcctgtacac ggcttttatt
     6661 ttcgctttaa ttccattttt tgcactcttt acttttgcat acttctgttt aaaaaatata
     6721 tcttctattg taatatctgg aaataaaggg attaaaagat ttttaatctt aagtttttct
     6781 ttatctgaaa attgtgtctt cccactttct ttgttgtaat aactctgctt agatatacca
     6841 agtttttttc caagtttctc ttgtgttaaa cctagcatat tcctatatcc acgcatcttg
     6901 ttttctttac tcatttctat acctccttta cttttgcata cctagattat aaaactttat
     6961 ttttataaag tcaatacttt tctttacttt ttcttaaaaa aagtttatta tgttctttaa
     7021 ggaggtgtaa aacatgaaaa taaataataa actcgttgga gaacgtattc aaaatatccg
     7081 tttaagccat ggcgactcta tggaaaaatt tggagaaaaa tttaatacta gcaaaggtac
     7141 agttaacaac tgggaaaaag gtcgcaattt accaaataaa gaaaacctac taaaaattgc
     7201 atctattgga aaaatgagtg ttgaagagtt actctacggc gattacaata cttatctaca
     7261 cttaaagatt atggatttag ctcctgaatg tataaaaaat tatgatgagt ataactcttt
     7321 acacgatgat ataacaaata aagcgttaca gatcgctcaa aataccattt ctaagattga
     7381 ttatcaaatt tcagacgaaa cgatcaaaaa atttattgat ttagctatcg aacaatcgag
     7441 agatttgcaa ggaaatttgt tgaaaaataa cttatctaat tttgactatt cactttttgg
     7501 aggtactaga aaccgagaat atttctattt tgggtataga cttacctata aagaaaaata
     7561 tctatacact gccttactag ctatactgaa cgagtcaaca aaaacgatat atattgatga
     7621 tataaataca gcaagatttt ttaacttctt tggcaatgat attcctaatt cgtctttaaa
     7681 aaaaattgac tatatcgcac cttcagaaat tgtttcattt agtacgtacg ttcgacaacg
     7741 ttctaaagta attcctaaaa ttttggaaca tatattaaaa tcaagttttt tattagagaa
     7801 tatagatgtt tctggttaca ctgtaaatat tttagaagat caattaacaa aacatagaac
     7861 aatcaaaatt agtaaaaact aactggttga tctcatgtat aaatacctaa caaaaccacg
     7921 cgccttgcct gctgatggaa agaaaggtac aaatacatga atatcaaaga aaaaatcaaa
     7981 aagaatggcc aaagagttta ttatgctagt gtttatctag gcgttgacca actaacgggc
     8041 aaaaaagccc gtacaactgt tacagcaacc actaaaaagg gcgttaaagt aaaagcgcgt
     8101 gatgcgatca atacttttgc tgctaatggc tatacagtta aagacaagcc gacaattaca
     8161 acatataatg agcttgtaaa agtttggtgg gatagttaca agaatacagt taagccaaat
     8221 actcgccaat ccatggaggg attggttaga gtgcatttat tgcctgtatt tggcgattac
     8281 aagctatcta aacttactac gcctattctt caacagcaag taaacaaatg ggctgacaag
     8341 gcaaataaag gcgaaaaagg ggcatttgct aactactctt tgctccataa catgaataag
     8401 cgtattttga aatatggcgt agctatccag gtaatacaat acaacccagc taatgatgtc
     8461 atcgttccac gcaaacagca aaaagaaaag gctgctgtca aatacttaga caacaaagaa
     8521 ttaaaacagt ttcttgatta tttagatgct ctggatcaat caaattatga gaacttattt
     8581 gatgttgttc tgtataagac tttattggcc actggttgcc gtattagtga ggctctggct
     8641 cttgaatggt ctgatattga cctagaaagc ggtgttatca gcatcaataa gacactaaac
     8701 cgctatcagg aaataaactc acctaaatca agcgctggtt atcgtgatat accaatagac
     8761 aaagccacat tacttttact gaaacaatac aaaaaccgtc aacaaattca gtcttggaaa
     8821 ttaggccgat ctgaaacagt tgtattctct gtatttacgg agaaatatgc ttatgcttgt
     8881 aacttacgca aacgcctaaa taagcatttt gatgctgctg gagtaactaa cgtatcattt
     8941 catggtttcc gccatacaca tactactatg atgctctatg ctcaggttag cccgaaagat
     9001 gttcagtata gattaggcca ctctaattta atgatcactg aaaatactta ctggcatact
     9061 aaccaagaga atgcaaaaaa agccgtctca aattatgaaa cagctatcaa caatttataa
     9121 aaaataaggg tgacccattt ccgggctacc ctcttactat accaaaaatt agtaggggta
     9181 gtaaaaaggg tattaaatta taaaaagcac taagggaaag cgccccaaag tgcttatttc
     9241 aaaggcttta tagcctataa tcacataaag agattatttt ttaaggttgt agaatgattt
     9301 caatccacga tattcagcta cttcaccaag ttggtcttcg atacgaagca attggttgta
     9361 tttagcgatg cggtctgtac gtgaaagtga accagtcttg atttgtcctg cgttagttgc
     9421 aactgcaata tcagcgattg ttgaatcttc agtttcacct gaacggtgtg atacaacagc
     9481 agtgtaacca gcttctttag ccatttcgat agcttcaaaa gtttcagtaa gagtaccgat
     9541 ttggttaact ttgataagga ttgagttagc agcaccttct tggataccac gtgcaaggta
     9601 gtcagtgttt gttacgaaga agtcgtcacc aacaagttgt actttcttac caagacgttc
     9661 agtaagagct ttccaaccat cccagtcgtt ttcatccata ccatcttcaa tagtgatgat
     9721 tgggtatttg ttaaccaatt cttcaaggta gtcgatttgt tctgcagatg tacgaacagc
     9781 agcaccttca ccttcaaatt tagtgtagtc gtaaacttta cgttctttat cgtagaattc
     9841 tgatgaagca cagtcaaatc cgataaatac gtctttacct ggtacatatc cagcagcttc
     9901 aatcgcagca aggatagttt caacaccatc ttcagttcct tcgaaacgag gagcgaatcc
     9961 accttcgtca cctacggcag tttccaaacc acgtgattta aggattttct taagagcgtg
    10021 gaagatttca gcaccgtaac gaagggcttc tttaaatgtt ggcgcaccaa ctggcaagat
    10081 catgaactct tggaaagcga ttggagcgtc agagtgagaa ccaccgttga tgatgttcat
    10141 cattggagtt ggaagaactt tagtgttgaa tccaccaaga tagctgtaaa gtgggatttc
    10201 aaggtagtca gcagcagcac gagctacagc gatagacaca ccgaggattg cattcgcacc
    10261 caatttacct ttgttaggag taccgtcaag tgcgatcata gcacggtcaa tagcttgttg
    10321 atcacgtaca tcgtagccaa tgatagcttc agcaatgatg ttgtttacgt tgtcaacagc
    10381 tttttgtgta ccaagaccac cgtaacgaga tttgtcaccg tcgcgaagtt caactgcttc
    10441 gtgttcacca gtagaagctc ctgatggaac cataccacgt ccgaaagcac ctgattcagt
    10501 gtaaacttct acttcaagtg ttgggttacc gcgtgagtct aggacttcgc gagcgtaaac
    10561 atcagtaata attgacattt tttactctcc ttatgagtta aattttttac acctctataa
    10621 taccttaaaa cccctccttt ttcaagaaaa aacgttatct ttgtgcaact tttccttaac
    10681 tttataaagt aatcgctttc ttttgtctgt tttattctaa cttttatgat atactgtttt
    10741 catgacagat ttatcaaaac aattacttga aaaagctcat ggtgggttaa aaataaatcc
    10801 ggatgagcaa agacgctatc ttggtacttt tgaggaaaga gttcttggat atgtagatat
    10861 tgacacagca aatagccctc agttagaaaa aggcttttta tttattttag aaaaccttca
    10921 ggaaaaagca gagccactat ttgtgaagat ttcaccaact atcgaatttg ataagcaagt
    10981 tttctactta aaagaagcaa aagaaactga tagtcaagcc accatagtat ctgaagagca
    11041 tattacttct ccttttggcc tggttattca tagcaatgca ccagttcaag tagaagaaaa
    11101 agaccttcga cttgcttttc caaaactttg ggaagttaaa aaggaagaac cagccaaaac
    11161 atccttatgg aagaaatggt ttagctaaat cttgcacata tttaataagt gcccaatatt
    11221 ggcagccgtg cgctccagat agaaactggc atttttcaaa ctatcttcta aaggttcact
    11281 tttctccaaa atagaaaaga cagcttggat attttcaaat ggtaggggag gtaaatcttc
    11341 agcaagacta ccgcaaatag caataacagg aactccaaca ggggttcttt ttgcaacacc
    11401 tataggcgct ttcccagcaa agctttgact atcaagtctt ccttctccaa caacaaccaa
    11461 gtcagcatct gaaactttct tatcaaagtt gattaagtcc aagcaggtat caattccaga
    11521 cacgatactt gcctgagcaa aggcacacaa accaccagca aggcctccac ctgctcctgc
    11581 tcctttaatt tctaatgttg caggtgagaa tttttcataa aaatcttgga tcgcctgatc
    11641 tacgactgca aacatagtcg gatgtagacc tttttgattg ccaaaagtgt aagtcgcacc
    11701 ttgatgacca cataagggac tcacgacatc tgctaaaata tgaatttgaa caccttcagg
    11761 aattttatag caattttctg ttgaaacaga agctaagttt aataaggatt gaccggaagc
    11821 aggcaagaca tttccatccc tatcataaaa ttgataacct aaaccagcag caatccccag
    11881 tcctccatca ttactggccg tgccaccaac accgatataa atatctttaa tccctttaga
    11941 gatgagatga agaatcaact ctccaatacc acaagtttgg atttgaagtg gatttcgttt
    12001 ctctagcgga atttttccaa gaccaaccaa gtcagctact tcaaatagtg ccagttcccc
    12061 tttttgaaaa tagcgcatgg cttctttttg tccaaaaggg tctgtcactt ggatccattt
    12121 ttcttttagg tcaagagaat gtcggatagc atctacagta ccttctcccc catcaccaac
    12181 agggcagagg agacattcta catctgctat cgattgttgg aagcctcttt ttattgcttc
    12241 agctacctgt tgagctgtca agctttcctt aaacgaatcc ggtgcaatta caatcttcat
    12301 attttccctc attctaaaca gtcaatcaaa gggagaactt ctaaaaaatc cctcttgtca
    12361 acatgatgtg gtatttcttt tttgagcact tctttggcac aaaaggcgat tcctaacttc
    12421 gccgacttca acattaatag attattaacc ccatcaccga ttgccaccgt tctttcttta
    12481 gaaagtttta gtttctttct ccatttttcc agagtctctt ttttgacctg gggacttata
    12541 atttgtccaa ctaattttcc tgttaaaaga ccttctttga cttcaagcta gttggcagtg
    12601 aaataggcaa taccaaggga ttttgctaat ctctccaact attggtgtaa atccaccaga
    12661 caccagacca actaggatgc cattcttttg gagaatagag atgaactctg ggacatttag
    12721 cgatagatga attgagttga agacgttatc aaagaccaaa ataggaagac cttccaacaa
    12781 ggacactctt tttcttaaac tgctttcaaa gaccaactct cctcgcattg ctcgacttgt
    12841 aatctgcgaa atttccgcct catgacctgc ctctctccct aaaagatcaa tcacttcttc
    12901 taggattaag gttccatcta catccaaaac acacaagcct tttacttgag acatcagttc
    12961 tcctctctaa acagcctaaa aatcgtatga agtcatcata cgattttatc tattaattaa
    13021 ctaaactatg gtacaagtca aggtatgact tgcaggctgt atcccatgag aagtcactct
    13081 ccatagcttg tttttgtagg tttctccaaa tgtctggatg gtttctatac aagtccaatg
    13141 ctgtttggaa agtccaattt aaccaataag gagatagatt gtcaaagcta aagccagtac
    13201 cgcttccttc gattggattg aaagcgcgaa ctgtatctcg caagcctcca acttcatgga
    13261 ccaatggcaa ggttccataa cgcatagcca tcatttgaga caagccacac ggttcaaaac
    13321 gacttggcat gaggaagagg tcacaagcag cgtagatttc ctgagcaagt ttgacatcaa
    13381 aagtgatatt tgttgatagc ttgtctgggt aaatctgagc aaaccatgag aaagctcctt
    13441 caaaggctgg atcgccagtt cccaaaagaa caatctgaac atcttcttgc aagatatggt
    13501 gaagactttc gaccaccaca tcaaaacctt tttgacgtgt caaacgagaa acaattccca
    13561 ccagtggaac gtctgctcta acaggcaagc caactctttc ttgcaatttt gccttatttt
    13621 tggctttccc agacaaatct tcctgattga aatgatagtc taaaagagca tccgtctgag
    13681 gattataaag atcagcatca atcccattca cgataccaga tactttacca gactccattt
    13741 taagaatctg atccaaatta catccaaact gactagtcat aatttcatga gcatagctag
    13801 gtgaaacggt tgaaacacgg ttcgcataga gaatacctgc cttcatccag ttcagacagt
    13861 tgttccatcg aagggtgcca tcagcgtaac gttcaaagcc aactccaaac aaatcaccca
    13921 acattccttc tgaaaattgt ccttggaatt ctaaattatg aatggttaaa actgtttcaa
    13981 tgtcctcata ggcttgaatc caacggtatt tttccttcaa caagaaagga atcatagctg
    14041 tatggtagtc atgaacatgg agaagatcag gaataaagtc aatcctttcc atagcctcaa
    14101 tggcagccag ttggaaaaag gcaaagcgtt ctccgtcatc aaaatcaccg taaacatgac
    14161 cacggaagaa ataatattga ttgtcaataa agtagaaggt tacaccattt aatactgttt
    14221 tcttaattcc acaatactgt ctgcgccaac caacgctcac ctcaaaatga agcacatctt
    14281 caatctgatt tccaaattta gcctctacca tatcatagta gggtaaaatc actgcaactt
    14341 cgtgcccagc ttttaccagt gattttggaa gagcgccaat gacgtctccc aaaccacctg
    14401 tttttgaaaa gggtgcaccc tctgctgcta caaataaaat tttcatgaat gaatatcctc
    14461 tgttacttta gcacctttct taaccacaac tggatgttct gcagttcctc gaatcacaac
    14521 accatgctca acttcaaccc ctttgtccaa gatagcatat tcgacctgag ccccttctcc
    14581 aataacaaca cgagggaaga gcaggctatc tttaaccaag ctatccttat ggacatgaat
    14641 attacgtgat agaacagaat tagctacttg accttcaata atactaccag aggcaaactg
    14701 agaagtgctt accttagatg tattagcata gtaagttggc tcttcgtttt tgacctttgt
    14761 ataaatcttt tggtttggtg agaaaagaga atagaatttt tgtgattcaa gcatatcgat
    14821 attcgcttga taataagatt taacagagtg aatattggct agatagcccg tgtactcgta
    14881 ggcgaaagct ccctctttta cagccaaatc ccgtaaaaca tagcgcaatt tctctggatg
    14941 ttctttttta gcttcttctt ccaagtgttc aatcaaccaa ggtgtatcaa cgacaaagat
    15001 atctgtagac atattgaacg tttcagctgt tgacttgcta tcaaagagtt tatgagaaag
    15061 aacatggtct gtttcatcta catccaagat tgcatttact tctgaaatat ctttcttagc
    15121 tagtttttta taaactacag tgataggctc ttttgttgta ctatgtaggt ggaaaacttg
    15181 gttcaaatca atgttaataa gaacatcgca gttgagggca accgtttggt ttgagccaga
    15241 acgtttcaaa taagtaagaa gctgttggta gtattctttt ccaactgtac tactttctac
    15301 acgggtattg taaattccta gatagtaatg gctaagaagg gttgataagc cccactcgcg
    15361 tcctgaacga atatggtcaa atactgagct gatattatcc tgctggaaaa taccaaagac
    15421 actacgaaca cctgcattag caaggcttga aagtgggaag tcaatcaaac gatatttccc
    15481 accaaatggc aaacttgcta ctggacggtg gtccgtcaat gtcgacatat tgtgaaaacc
    15541 aactgtattt cctaaaatgg cagaatattt atcaatcttc atctgttgct acccccacta
    15601 cttcattata tcctacaact tgtacttcat ctgttccatc aatttcgaca ccgtcagaaa
    15661 taatcgcacc ttcaccaata atggcacgtt taatcttagc tccttgacca atgatagctc
    15721 cactcatgat aactgaatca aggacttccg ctccttcgcg aacttgcgcg cctgttgaaa
    15781 ggatagaatg tttaacagtt ccatcaacga aacatccgtc tacaactaat gagtcttcca
    15841 catgagcatt tgccccgagg aagtttggtg gtgaaatcaa gtttcttgag taaatcttcc
    15901 attgacggtt acgactatcc aaggcatttt ctggagaaat atactccatg ttcgcttccc
    15961 aaagtgactc aatagtacca acatctttcc aataaccact aaattcgtaa gcataaacac
    16021 tttcacctga ctcaaggtaa tttggaatga catttttacc aaagtctgac atgccaacct
    16081 tgctcttttc agcagcgact aacatattac gaaggcgttg ccaatcaaaa atgtagattc
    16141 ccatagaagc ttttgtagat ttaggttgag ctggtttttc ttcaaattca acaatacgat
    16201 tgttagcatc tgtgttcatg ataccaaaac ggcttgcttc tttaagaggg acgtctaaaa
    16261 ctgctactgt caagctggca ttattatcct tatgagactg gagcatatca tcatagtcca
    16321 ttttgtagat gtgatcccca gacaaaatca agacatactc aggattgaca ctgtcgatat
    16381 agtcgatatt ttggtaaata gcgtgactag tcccctcaaa ccaacgattt ccttcacttg
    16441 cagaataagg ttgaagaata gagacacctg aattaatacc gtctagtccc cagcttgaac
    16501 cattcccaat atggttgttg agagcaagtg gttgatactg tgtaacgacc ccaacattgt
    16561 gaatccctga gttggcacag tttgataggg caaagtcaat gatacggtag cgcccaccaa
    16621 attgcacagc tggttttgcg atgctttgag tgagtttacc gagacgagtt ccttgcccac
    16681 cagcaagaat caaagctaac atttcatttt tcattttcta ctcctttttg gtttttattt
    16741 gtgacggttt tagtagattt caagcgacgt ttgattttcc atacacttgc tcccatagcc
    16801 ggtagggtaa aggttaaggt ctgctcataa tctttccata gtccttcttg cgtttgaaca
    16861 gtttgattat gttctttcca aacgcctccc cactcttcca actcagtatt ccatacttct
    16921 tcgtaaattc ctgcaacggg tagtccgatt gtaaaatctt tccgctcaac aggtaccata
    16981 ttaaagatac agactaacat ttctcccttt ttacccttac gaataaagga aagaacactc
    17041 tggtctcgat tatccgcatc aatgatttca ataccatcat agctggtatc aatttcccac
    17101 agacagcgat gatctttgta aaactggttt agctgagaag cgaaatactt catcttagca
    17161 ttcattgggt cttctaggtt agaccattcc aactgttctt cagatttcca ttctaggaat
    17221 tgaccgtatt cgctacccat gaagagcaat ttcttaccag ggtgacaaat ttggtacgta
    17281 tagagattgc gcaagcctgc gaattgattg taacgatctc cccacatctt atgcatcata
    17341 ctcttcttgc catgaaccac ttcatcgtgc gagaatggca agagataatt ctccttgaaa
    17401 acatacataa agctgaaagt caccaggtta aagtcatatt tacgatagat cggatcttct
    17461 tcgtagaaac ggaggatatc attcatccag cccatgttcc atttgtagtc aaatcctaga
    17521 ccaccaatct ctttcattcc cgtaatcttg atcgcagacg aactttcttc tgcaatcatc
    17581 atcacatctg gatattctaa cttaataacc tcattcaagc gctgaaggaa ataataacct
    17641 tcatagttga gatttccgcc atctttatta ggtgtccatg gagcatcatc atagtccaaa
    17701 tagagcatgt tgctaacagc atccacacga ataccatcca aatgatagac atcaatccaa
    17761 tgcttaatgc aagaaattaa gaaggactgg acttcatttt ttccaaggtc aaaattaagg
    17821 gcaccccaac catggttatg agccttatta tggtcttggt attcaaaagt cggtgtccca
    17881 tcataatagg ctaaggcatc atcgttgatg gtaaagtgac ctggtaccca gtccacaata
    17941 accccaatat tatgggtatg acactcctcg acaaaatctt gaaactcctc tggtcggcca
    18001 taagcatgct ctaaagcgaa gtaacccata agctgatacc cccaactcaa gcccaaagga
    18061 tgggacatca agggcataaa ctcaatatga gtatagttca tttcaacgag ataaggaatg
    18121 agttcatcct tgagctgggc aaaactataa ggactgccat cagaatttct tttccatgat
    18181 ccagcgtgaa cttcataaat attgacagga cgctcttcaa agccccaacg ttttcttcgt
    18241 gccagccaaa gtccatcctt ccatttcttc tcaggaagct ctgttacgat tgcccctgtt
    18301 cctggacgag cctcatacct gacagcaaaa gggtcaatct tcatcagttg atgaccattt
    18361 tgacgtgtga catgatattt gtaaatatgc ccttcttgag ccatattggt aaagacttcc
    18421 cagaccccaa aatcatttct taccattgga atctgatttt caatccagtt ggtaaaatca
    18481 ccaaccaagt gaacagcctg agcattaggt gcccaaacac ggaaggtata gccatgctct
    18541 ccatttagtt cttccctatg tgctcctaga taatgttgga gataaaaatt ttcacccgtc
    18601 ataaaggttt ttaatgcttc tctattatcc atatactccc cttctcctgt aagcgttttc
    18661 tatgttttta ttatactacc tttttagaga agattcaagt aaattactat acttctttaa
    18721 ttattttgaa aatctacaac aagttcactt actcgttcaa ttgtaaatca atattttttc
    18781 aaaaaattgc gaaaacgcct ttcttttt
//
LOCUS       DAVKNL010000040        18715 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVKNL010000040 DAVKNL010000000
VERSION     DAVKNL010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18715
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.040"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(281..1087)
                     /gene="yidA"
                     /locus_tag="VW411_001410"
     CDS             complement(281..1087)
                     /gene="yidA"
                     /locus_tag="VW411_001410"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEU9794129.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDEGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYERYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            complement(1100..2404)
                     /locus_tag="VW411_001411"
     CDS             complement(1100..2404)
                     /locus_tag="VW411_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU9794130.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFEHLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSHRFVNRKVFKSITFSQEDQDQLTSMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            2476..2853
                     /locus_tag="VW411_001412"
     CDS             2476..2853
                     /locus_tag="VW411_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEU9794131.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            2944..3276
                     /locus_tag="VW411_001413"
     CDS             2944..3276
                     /locus_tag="VW411_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEU9794132.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            3288..4859
                     /gene="ffh"
                     /locus_tag="VW411_001414"
     CDS             3288..4859
                     /gene="ffh"
                     /locus_tag="VW411_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEU9794133.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(5054..6337)
                     /locus_tag="VW411_001415"
     CDS             complement(5054..6337)
                     /locus_tag="VW411_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEU9794134.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(6640..7353)
                     /gene="rsmG"
                     /locus_tag="VW411_001416"
     CDS             complement(6640..7353)
                     /gene="rsmG"
                     /locus_tag="VW411_001416"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEU9794135.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            7447..8007
                     /locus_tag="VW411_001417"
     CDS             7447..8007
                     /locus_tag="VW411_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU9794136.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            8009..8908
                     /gene="htpX"
                     /locus_tag="VW411_001418"
     CDS             8009..8908
                     /gene="htpX"
                     /locus_tag="VW411_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEU9794137.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(8952..10511)
                     /locus_tag="VW411_001419"
     CDS             complement(8952..10511)
                     /locus_tag="VW411_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9794138.1"
                     /translation="MHYEHSRKGNHMIKINHLTITQNKDLRDLVSDLTMTIQDGEKVA
                     IIGEEGNGKSTLLKILMGEALSDFTIKGNIQSDYQSLAYIPQKVPEDLKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLQRLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYLSKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(10750..11067)
                     /locus_tag="VW411_001420"
     CDS             complement(10750..11067)
                     /locus_tag="VW411_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEU9794139.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EQLNNSILIDLEKFYQPTSLLIGLGSLKLNDQARTAWRNYDKFHYDHVKHVLSLYGPV
                     FEF"
     gene            11237..11779
                     /locus_tag="VW411_001421"
     CDS             11237..11779
                     /locus_tag="VW411_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEU9794140.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGAANQLDQEVLDDD
                     LVLPIENGELDLAESVSDNILLNIPIKVLTAEEEAGQGFISGNDWQIMTEEEYQAQKA
                     VKKEENSPFAGLQGLFDGDE"
     gene            11779..12408
                     /gene="nth"
                     /locus_tag="VW411_001422"
     CDS             11779..12408
                     /gene="nth"
                     /locus_tag="VW411_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU9794141.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            12619..13140
                     /gene="pyrR"
                     /locus_tag="VW411_001423"
     CDS             12619..13140
                     /gene="pyrR"
                     /locus_tag="VW411_001423"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEU9794142.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            13159..14082
                     /locus_tag="VW411_001424"
     CDS             13159..14082
                     /locus_tag="VW411_001424"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEU9794143.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            14132..15211
                     /gene="carA"
                     /locus_tag="VW411_001425"
     CDS             14132..15211
                     /gene="carA"
                     /locus_tag="VW411_001425"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEU9794144.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            15524..18700
                     /gene="carB"
                     /locus_tag="VW411_001426"
     CDS             15524..18700
                     /gene="carB"
                     /locus_tag="VW411_001426"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU9794145.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKEGPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDKVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
BASE COUNT         5448 a         3606 c         4202 g         5459 t
ORIGIN      
        1 agtatcctaa tttgtataat tgattgtgtg tagattcagg gtcaatttga agagagtatt
       61 taaaatatct tgggaaatta taatttctcc ctgttttttg agtccaatct atgagtatga
      121 gttgcccagg agtaaggcca cattctaatt cgtttaggta aaaattgtta caatcagaat
      181 ttgtgtctct attatgatta ttttgtttta aattaaatag tttgctaaaa aaattcattg
      241 gattttattg ctaatcaatt cttattgaaa aatgatactt ttacagtacc catgttcgga
      301 tggcatgggc aacgccggat tcgtcatttg ttttggtgat gtatttggcg atttttttga
      361 tttctggatt tccattttcc atgacaacgg ggtttccaac gacttccagc atggcacggt
      421 cattttcttc atcaccgatt gccatggttt catctttggt caatccgagt ttttcagcca
      481 agtgagtaat ggctgaaccc ttgtctacat tctttttaag gagttcgagg tagaaaggag
      541 cagatttgtt gatggagtag cgctcgtaaa attctgctgg aattttttca atcgcagcat
      601 cgagaatttc tggttcatcg ataaacatac atttaacaat ttctttgcca gccatttctt
      661 caggggtacg gtagaagata ggcatgctga cgagggttga ttcgtgtaca gtgtattttc
      721 cgatattgcg atttgcagta tagataccgt ccttggtaat ggcatgcatg tggacaccga
      781 gcttgcgact gaggaattcc atatctagat aatcctcata agtcaaggat tcgctgataa
      841 tctcatgtcc tgtagcagtt tcttggacaa gggcaccgtt gaaggttacc acatagtccc
      901 cctcgtctct caactgcaag tcgtctagaa gtttggcaac gcctgcgata gggcggccag
      961 ttgcaatcac gactttgaca ccagcttctt tggcatcttg gatggcagaa aaaacttcag
     1021 gagtgatttc cttttggctg ttgacaaggg ttccgtcgat atcaacggca attagtttaa
     1081 tactcataaa tttcctcttc tagtttttat ttggggtaaa atgatcgttc tcaatcaagt
     1141 gtaaaaattg ctgggtaatg cttgcaaaga tgctgttttg gtccaacatt tcttttggaa
     1201 aataaaagcg attatctccg tggcgactgc cagcaaggga ttggacgata ggagacaggc
     1261 tagagagttc ggccagttct ccattttttt gtaaaatctc aatctgtgtc cgtgggtttt
     1321 cagattcggg acgatagata tcataaggga ggtcaaagtt cttatgaatg gcagtgtagt
     1381 agtcgggatc aaagccgata tcctcaacca attttctcat gctagtaagt tgatcttggt
     1441 cctcttgtga aaaggtaatg gatttaaaga ccttgcggtt gacaaagcga tgcgataaat
     1501 ctgcaagaat cttgtcagga ctggtcatcc aaagctggaa gtaggtattc atcacgccat
     1561 catccagagc cagatagtca gtcaaggtca catttttttc gaagaaaggc aggaggtgtg
     1621 gagaagttcg ggcaaagaaa tccttgtcct caggatagag ttccttggcg cgtttgagaa
     1681 gattctgtag gagaacttcc atggcgcgtg ttgcggggtg gaaataaacc tgcatgtaca
     1741 tctggtagcg actgaggacg tagtcttcga tggcgtgcat gccattgcgc tgaaaggcga
     1801 taccattttc gataggacga atgactcgga ggattcgagt caggtcaaat tccccatagg
     1861 atgctcctgt aaaataggag tcgcgcaaga gatagtccat gcgatctgcg tcaatctgac
     1921 tagaaatgag ctgcacgacc tgcttattag gataggtatg gtcaatgaca ctggccacct
     1981 tttctgggaa atcaggtgcc acttgtagca ggacttggtg aatctctgtc tcaggatttt
     2041 gaataatctc ctgagtaatg gcttcatggt ctgtatcaaa gagatgttca aaagtatggg
     2101 agtaggcacc atgcccaagg tcgtgtagga gagcagcggt catggtcaag agagactcgg
     2161 caggattcca ttcctcagga tatttttctt cgaaaatctc tgtgatgcgt cgtgcaattt
     2221 catagactcc tagacagtga gagaagcgac tgtgttctcc accgtggaag gtataactgg
     2281 aagttcccag ttgtttgatc cggcgcaaac gctgaaattc ttttgtatta atcaagtcat
     2341 agatgatttg attattgaca tggatgtagt tgtgaacagg gtcacggaat actttttcgt
     2401 tcatatgacc attatagcaa aatcctgctc tttttggtaa aatagtagga caagagacaa
     2461 gaaagagggc ctgatgtgaa gattcggatg cgaaatacga ttcagtttga tgagcagttg
     2521 gaagtgattg accagcttta tgatgtggaa gtgcatgaaa aaggagatta tagctacctg
     2581 cttttctata atgaggaaaa ggaaaaagta gtgattaaat ttcatggtca agaacttgtg
     2641 atgagccgat tttctaatcc caagaccatt atgcgctttc taaaggatag cgatagttta
     2701 gcctatattc ctacacctat gggcatgcag gagtttatca tccaaacgag ccattatcaa
     2761 gttgataggc aaaagattga actagattat caactacaaa atcaagaggg acatcccttt
     2821 gccagctatc aattggaaat tacttggggc taggctcatg tctttttcaa atttagctat
     2881 ctatcttctt ggatcggctt cctagtgtgg tagattaggc taatttttgg tataataaaa
     2941 gttatggaaa tcgaaaaaac caatcgtatg aatgcgctct ttgaatttta tgcggcgctt
     3001 ttgacagata agcaaatgaa ttatatcgag ctctactacg ctgatgatta cagccttgct
     3061 gaaattgccg aggagttcgg tgtcagtcgt caggctgtct atgacaatat caagcgaaca
     3121 gaaaagattc tggaagatta tgagatgaaa ttgcacatgt actcggacta tattgtccgc
     3181 agtcagattt ttgatcagat tttggagcgc tatcccaagg atgactttct gcaggagcag
     3241 atagaaattt taacaagcat tgataataga gaataagagg aagaaaaatg gcatttgaaa
     3301 gtttaacaga acgtttgcag aacgtcttta aaaatctacg taaaaaagga aaaatctctg
     3361 aatctgatgt ccaagaggca accaaagaaa ttcgcttggc cttgctcgag gccgacgttg
     3421 ccttgcctgt tgtaaaggac tttatcaaga aagttcgtga gcgtgcagtc gggcatgagg
     3481 tcattgatac acttaatcct gcgcaacaga ttattaaaat cgttgatgag gaattgacag
     3541 ccgttttagg ttctgatacg gcagaaatta tcaagtcacc taagattcca accatcatca
     3601 tgatggttgg tttacaaggg gctggtaaaa caacctttgc tggtaaattg gccaacaaac
     3661 tcaagaaaga agaaaatgct cgtcctttga tgattgcggc ggatatttat cgtccagctg
     3721 ccattgacca gcttaagacc ttgggacaac agattgatgt gcctgtcttt gcacttggaa
     3781 cagaagtacc agctgttgag attgtacgtc aaggtttgga gcaagcccaa actaatcata
     3841 acgactatgt cttgattgat actgcgggtc gtttgcagat tgatgagctc ctcatgaatg
     3901 agcttcgtga tgtgaaagca ttggctcaac caaatgaaat cttgcttgtc gttgatgcta
     3961 tgattggtca ggaagcagcc aatgttgcgc gtgagtttaa tgctcagttg gaagtgactg
     4021 gggtcatcct taccaagatt gatggcgata ctcgtggtgg tgctgctctg tctgttcgtc
     4081 acattactgg aaaaccaatc aagttcactg gtacaggtga aaagattacg gacattgaaa
     4141 ccttccaccc agaccgcatg tctagccgta tccttggtat gggggatatg ctcactttga
     4201 ttgagaaagc ttctcaggaa tacgatgaac aaaaagccct tgaaatggct gagaagatgc
     4261 gcgaaaacac ctttgatttt aatgatttca tcgatcaatt agatcaggtg caaaatatgg
     4321 ggccgatgga agacttgctc aagatgattc caggtatggc caacaatcca gcccttcaaa
     4381 acatgaaggt ggatgaacgc cagattgctc gtaaacgtgc cattgtgtct tcgatgacac
     4441 ctgaagagcg tgaaaaccca gatttgttaa atccaagccg tcgccgtcgt attgctgctg
     4501 gttctggaaa tacattcgtc gaagtcaata aattcatcaa ggactttaac caggctaaac
     4561 agctcatgca gggtgttatg tctggggata tgaataaaat gatgaagcaa atggggatta
     4621 atccaaataa ccttcctaaa aatatgccaa atatgggagg aatggatatg tctgcccttg
     4681 aaggaatgat gggacaaggc ggtatgcctg acttatcagc tctcggagga gcaggaatgc
     4741 cagatatgag ccagatgttt ggtggcggtt tgaaaggtaa aattggtgaa tttgccatga
     4801 aacagtccat gaaacgtatg gctaacaaaa tgaagaaagc gaagaagaaa cgcaagtaag
     4861 acaagagatt gatgcaaatc tcatttgatg agaaaataaa gtaaaaattt ctatgataaa
     4921 acgtacagta tcaaggtttt agaatatctg atattatacg ttttttgatt ttaataattt
     4981 tgttaagaca cttttattat ggtatacgaa aaatccactc agctgtctga gtggattagg
     5041 tgtatttaga ctcttagtct ttattttcgt atggcaagat caagttcaag atgattcctg
     5101 tcatggctga aagggcagta cctgaaagtg taactggacc aagtttaagg atagctcctc
     5161 caagtccaag aaccaacata gcacttgcga tgatgaggtt tcgcatttga gcgaaatcaa
     5221 cacgttcttt aatcaagact ttcaaaccat tgctggcgat aaccccatag agaaggattg
     5281 acataccacc aagtacagcg tttggaatag ttgaaatcaa ggcagtgaat ttaccaagga
     5341 agctgagggc aatcgcgatg aaggcagcgt tacggataac tgagacagaa gcgatacgag
     5401 tcataccgat aacccctgta ttttctccgt aagttgtatt ggctggtcca ccaaggaagg
     5461 cagaaacaga agttgcgata ccgtcaccaa gaagagtacg gtgaagacct ggttctttta
     5521 agaattgacg accacagatt tgacccaaaa cagtatggtc tccgatatgt tcagaaattg
     5581 ttacgatagc gattggcaag atagcgatgg cttctggacc aaagtaaaga ttgtactctt
     5641 taaaggcacc acctgtgcta aatggcaagt agaaaccagg aatttcgaac cagttggctt
     5701 taagaactgg tgtaaagtca accaagccaa gagttagtgc gaaaaggtaa ccaccgataa
     5761 tggcaaagag gaatggaatg attcgtagga agccttttcc ttttgtattg ataaaggcag
     5821 caattaggaa agtaacaacg gctaccagag catttttcca atttccgtct gctacaagac
     5881 ctgcattggt aacagctgaa cctgcaagtc caagaccgat aacgatgatc ataggaccga
     5941 taatgattgg tggcaagagt ttatcaatcc attttgttcc tacaaatcgg atgctggtag
     6001 caacaaggac atagaccaaa ccagtcaaga taacccctgt ttgggcagca gatacatccc
     6061 cccccatttc tttcatagcc agtgacatag ctgtgataaa ggcaaatgaa gaacctagat
     6121 aaactggaac tttaaaacca gtagcaatca tgtagatgag tgttccaaca cctgaagcaa
     6181 aaagggcaac agatacaggc attcccaaaa tcaatggtac caagatggtc gcaccaaaca
     6241 tggcgaaaac gtgttggaaa ctaaggagaa ttccttttcc agccgaagga cgttggtcaa
     6301 cgtctagtaa caaatcaaca gttgattctt gtttcatata aacctcactt ttgggcatca
     6361 aaaaagagcc cattatttta cagcaatagg ccctcagagt aagacttctt taaaatttag
     6421 attttaaaga gttttaaata tttctgcgtc ttcgtcacct cacgggatga cattaaaaac
     6481 ctttgcttag gatagtatag cagaaaaaaa tgattttgta aatcattttt tcccgagcct
     6541 agaaataaaa gagcgaggct gattttgtaa atcatttttt cccgagccta gaaataaaag
     6601 agcgaggctg attttgtaaa catttgttta ctaaaaaatt taaagtgggc gtttatttgg
     6661 cataccagcc ttacgtggat atttatttgg tgtttctttt ttcttttcta ccactgtgat
     6721 atagcgcgga tctctattcg gtagggcgta gctgagattg tcttcgacct tactaaaaag
     6781 gagattgagg gcattcttag cttctaataa ttcctcaggc gcattgctag ccttgagtgc
     6841 taatagtttg ccaccaacct taaggtaggg aatagtcaat tcagatagga cctgcatacg
     6901 ggcaaccgca cgagctgtta caaaatcata ttgagcacgg aagttcttgt cttgggcaaa
     6961 atcttcggca cgtccgtggt agaaatgaac tccgttcaaa tccagttctt gagccaagag
     7021 ttgtaggaag ttgatgcgct tattgagtga atcaataatg gtcacatcta actccggata
     7081 gagaattttc attggtagac taggaaatcc tgccccagcc ccgatatcaa gaagtttgat
     7141 agtttcattg ggaatcaaac cttgaagaat gggtgcaatc gaatcgtaaa aatgtttgag
     7201 ataaacttct tccttgtccg taatcgccgt caaattaatc ttctcattcc actcgaccaa
     7261 gagctcaaaa taacgttcaa attgttcttt ttgctggtcc gaaagtggaa gattctgctc
     7321 ggcaagcaag ttgtaaaatg tttttggttt catagttatt tccttcttta gtatcatctt
     7381 attttaccac aatcatagaa aatttgatat actggtacta atctaaaagc agaaaggact
     7441 tatattatga cttggattat tcttggagtt atcgctctta ttgttatttt tgtgattgtt
     7501 agctataacg gtttggttaa aaatcgtatg caaaccaagg aggcttggag tcagattgat
     7561 gttcagttga aacgtcgcaa tgacctcttg ccaaacttga ttgagactgt aaaaggttat
     7621 gccaaatatg aaggttctac ccttgaaaag gtggcagaac tacgtaacca agtggcggca
     7681 gcgacttcac cagcagaagc tatgaaagcc agtgatgccc tcactcgtca ggtttcaggt
     7741 atttttgcag ttgcagaaag ctatccagat ttgaaagcta gtgctaactt tgttaaattg
     7801 caagaggagt tgacaaacac agaaaataaa atttcttact ctcgtcaact ctataacagt
     7861 gttgtcagca actacaatgt aaaattagaa actttcccga gcaatattat cgctggaatg
     7921 tttggattta aagcggcaga tttccttcaa acacctgaag aggaaaagtc ggttcctaaa
     7981 gttgatttta gcggtttagg tgactaagat gttgtttgat caaattgcaa gcaataaacg
     8041 aaaaacctgg attttgttgc tggtattttt cctactctta gctcttgttg gttatgcggt
     8101 tggttatctc tttataagat ctggacttgg tggtttggtt attgcactga ttatcggctt
     8161 tatctacgct ttgtctatga tttttcaatc gacagagatt gtcatgtcca tgaatggagc
     8221 gcgtgaggtg gatgagcaaa cggcaccaga cctctaccat gtagtggaag atatggctct
     8281 ggtcgctcag attcctatgc cccgtgtttt catcattgat gatccagcct taaatgcctt
     8341 tgcgacaggt tctaatcctc aaaatgcggc tgttgctgcg acttcaggtc tactagctat
     8401 catgaatcgt gaagaactag aagctgttat gggacatgaa gtcagtcata ttcgtaatta
     8461 tgatatccgt atttcgacta ttgcagttgc ccttgctagt gctatcacca tgctttctag
     8521 tatggcaggt cgtatgatgt ggtggggtgg agcaggtcgc agacgaagtg atgatgaccg
     8581 agatggaaat ggtcttgaaa tcattatgct agtggtttcc ctactagcta ttgtactggc
     8641 acctctcgct gcaaccttgg ttcagctcgc tatttctcgt cagagggaat ttctggcaga
     8701 tgcatctagt gtcgagctga ctcgcaatcc ccagggaatg attaatgccc tagataagtt
     8761 ggacaatagc aaacctatga gtcgccacgt cgatgatgct agcagtgccc tttatatcaa
     8821 tgatcctaag aaaggtgggg ggttccaaaa actcttttat acccacccac ctatctcaga
     8881 acggattgaa cgtttaaaac agatgtaaaa gaatccagag gtcgtctctg gatttttgct
     8941 atgttgcaaa tttataaatc ttctaaatta actagcttca aaccgtgttc tgtcatgcga
     9001 tagatgatcg agcagacttc ttttaagaaa cgacggtcat gcgaaacagt gatgagaccg
     9061 cctggatagg tagcaaagag ttttctgatt tggggttgag aagtgggaga aaagtttcgt
     9121 gtgggttcat ccagcaggag aaagtttggt ttgcgcagga ctaaatccaa aagcaggagt
     9181 tttccctgtt gtccgccaga taaggagcga atttgatgct gcatttctgg ataactgaaa
     9241 ttgagactag ctaggtgaga ttggattttc tgtagttcct ctttttcccc agttttactg
     9301 agataggcta ttggggataa atccaattgc agttttttgt ggtaatcttg tggcataaaa
     9361 ccaagtgaaa tctctctttt atcattcaga agtctctgta acttggctaa cagagttgat
     9421 ttcccaacac catttggccc gataataccg attttttctt ggccacggac agttagttgt
     9481 agtttttgaa ccaaaactcg gtcgtcaatg gacaaatttt ctttttccag ttggactaag
     9541 actttagaag ctggtaatgg ttggatgtct gaaaagaaaa gttggatttg ttcctcttca
     9601 agtggctttt gagtcatgga ctgagctgcc ttttcgtagc gtttttcttg tgagaggaca
     9661 gttttcatct ttttagccaa taggcgaccg gcagtactat ctttggtagc tcgaagcgca
     9721 gtttctacat tttgcttaac tctccgatgt ttttccatgg ttttatcgta ggctctttgg
     9781 ttgttagcag cttgctgact ttgtttggca aaattagcct ttctctgctc actatagcta
     9841 tcatagtcta aatgctctac tagcgtttcc gcttctttac ggtgtttgac cagtcgcaag
     9901 tgaacaatag tgtctgccgt ttcagaaaga aagtcttcat catgggaaat gaaaataacg
     9961 gtttgcctgg tcttttgaat ctggcctttt agccaatcaa ctgtctcaag gtctaggtca
    10021 tttgaaggtt catctaaaaa tagaatctca aagggtttgg ctaactcatg gataagctga
    10081 attttcaaag cttcgccccc tgatagattg ccaatctctt ggtcacttgc gaaacgattg
    10141 ctatcaaaat gcaattcctc cgccaaacga tagaggatac tgtagtctaa atcaatagaa
    10201 tctaaaaaga agtagtcgtg taaagttttc ttttttaggt cctcagggac tttttgagga
    10261 atgtaggcca gtgactgata gtcagattgg atgtttccct tgatagtgaa atcagacaaa
    10321 gcttccccca ttaaaatttt aagtaaggtt gatttgccat ttccttcttc accaataata
    10381 gcaacctttt ccccgtcttg gatggtcatg gttaggtcag atacaagatc tcgtaaatct
    10441 ttgttttgtg tgatggttag atgattgatt tttatcatat gatttccctt tctagaatgt
    10501 tcatagtgca taacaaaaag ctctacttta cctagtagag cccaaagtct atgtcaaaaa
    10561 aactctatta acatgccaga aaaagagcat aaaagagcac acaaaaagag acaaaaacaa
    10621 gatctactaa ataaaggttc tttatttgat agacttagtt gttcatgtct cccttacctc
    10681 cgagtattag tatctttata ttataccttt aaggaaattt tgtcaacaga aaactggaaa
    10741 ttctatgctc taaaattcaa aaacaggtcc atagagactt agtacgtgtt tgacatgatc
    10801 gtaatggaat ttatcatagt ttcgccaagc agtgcgtgct tgatcgttta gttttaggct
    10861 acccagtcca atcagaagac tggtaggttg gtaaaatttc tcaagatcaa ttaagatact
    10921 gttgttcaac tgttccgttt ttttgagctc ttgcatagta gtgtttagca gctcttgtag
    10981 tcgaatatca tctgccgtac cctgtttaca gttttggtag cttttattta acttggatag
    11041 gagttgaatg agtttcttgt cttccatgtg agcatcctcc ttgatctgat gtactctttc
    11101 cctatagtat agcactaaaa aataaaacta tcatagtaaa tatgttaaaa ttttgaattt
    11161 taattacaaa gtttggtgtg ctgatttgta gccttttcat ggtataatca agagagtaaa
    11221 tctttatgga ggaagtatga aattaaatat tcaagaaatt cgtaagcagt ctgaaggttt
    11281 gaactttgaa caaacgttag atttagttga tgacctgcgt gcacgtaatc aagaaatttt
    11341 agatgtaaaa gatatccttg cagttgggaa agtacaatat gaagaccgta tgtatttctt
    11401 agattatcaa ctatcttata ccattgttct tgcttcgagt cgcagtatgg agccagttga
    11461 gttagttgaa tcttatccag tcacggaagt tttcatggaa ggcgcagcta accagctaga
    11521 tcaagaagtt ttagatgatg acttggtctt gcccatcgaa aatggggagc ttgaccttgc
    11581 tgagagtgta tcagacaata tcctgctaaa cattcctatc aaggtcttga cggctgaaga
    11641 agaagctggt caaggattta tctcaggaaa tgactggcaa atcatgacag aggaagaata
    11701 ccaagctcaa aaagcagtaa agaaagaaga aaacagtcct tttgctggct tacaaggact
    11761 atttgacgga gatgaataat ggtcttgtca aaaaaacgag cacgaaaggt gctagaagaa
    11821 atcattgccc tcttcccaga tgccaagcct agtcttgatt ttaccaatca ttttgaactc
    11881 ctggttgcgg tcatgttatc agcccagacg acagatgcag cggtaaataa ggccacacca
    11941 ggtctctttg ttgcctttcc aacaccccaa gctatgtctg tagcgacaga gagtgagatt
    12001 gcttcacata tttctcgctt gggattgtat cggaataaag ctaaattcct taaaaaatgt
    12061 gcccaacagt tactagacga ttttgatggt caagtccctc agacacgtga agaattggag
    12121 agtttggcag gtgttggtcg caagacagcc aatgttgtca tgagtgtagg atttggaatt
    12181 ccagcctttg cagtggatac tcatgtggag cgtatttgca aacaccacga tattgtcaaa
    12241 aaatcagcga cgccacttga ggtggaaaag cgggtcatgg atatcttgcc gcctgagcag
    12301 tggttagctg cccatcaggc catgatttat tttggaagag ccatttgcca tccaaaaaat
    12361 ccagagtgtg accagtaccc acaattatat gattttagca atttgtaaga tgctacattg
    12421 aaactttttg cttgatttta agcatgcttt gtgctatagt aaatgataac taaatattcc
    12481 tttaaacctg tcccgtgagg caggcaagga gcgcgataaa gtagaagggt gaagtctata
    12541 ctgtgtgcag tagggctcgc ttggctataa atttcaagtc tccttgttta aggagacttt
    12601 tctttttgga ggtttgtcat gaaaacaaaa gaagttgtag acgaattgac cgtcaaacga
    12661 gcgattacgc gtattactta tgagattatc gaacgcaaca aagatttgaa taagatcgtc
    12721 ttggctggta ttaaaactcg tggtgtcttt attgcccacc gaatccaaga acgtttgaag
    12781 cagttagaaa atctttcagt tcctgttgtg gaattggata ctaaaccttt ccgtgatgat
    12841 gttaaaagtg gagaagatac ttctttggtt tctgtcgatg tgacagaccg cgaagttatc
    12901 ttggtggatg atgtgctcta tacaggtcgt accatccgtg ctgctattga taatattgtc
    12961 ggtcatggtc gtcctgcgcg tgtgagttta gcagttctag tcgatcgtgg acatagagaa
    13021 ttgccaatcc gtccagatta cgttggaaaa aatatcccaa ccagtcgttc tgaagaaatc
    13081 atcgtagaga tgacagaact tgatgaccaa gacagagttc tgattactga agaagcttag
    13141 aaagttaaag gagtagccat gtcagaaaat caacaagcat tgaaccatgt ggtgtccatg
    13201 gaagacctca ctgtcgatca agtaatgaaa ttgatcaagc gaggaattga gtttaaaaat
    13261 ggagcccagc ttccctatga agaccatccg attgtttcca atctcttctt tgaggattct
    13321 acacggacac ataagtcctt tgaagtcgca gagattaaac ttggattgga acgacttgac
    13381 tttgatgtga agactagctc ggttaataag ggtgagacac tttatgacac cattttgact
    13441 ctgtctgctt taggagtgga tgtctgtgtg attcgccatc cagaggtcga ctactataga
    13501 gaattgattg ctagtccaac gattacgact tccatcatca atggtggaga tggttcgggc
    13561 caacacccta gccagagctt gcttgatttg atgaccattt atgaggaatt tggccacttt
    13621 gagggtctta aagttgctat tgcaggtgac ttggaccact cacgcgttgc caaatccaat
    13681 atgcagattt tgaaacgctt gggagctgaa ctctttttcg ctggacctga ggaatggaga
    13741 agtcaagagt ttgcagacta tggacagttt gtaaccattg atgaaatcat tgatcaggtt
    13801 gatgtcatga tgtttctccg tgtgcaacac gaacgccatg atagtggagc tgtattttca
    13861 aaagaagact accatgccca acatggcttg actcaagaac gttacgatcg tttgaaagaa
    13921 acagcaatcc tcatgcaccc agctccaatc aatcgtgatg tagaaatagc agaccacttg
    13981 gttgaagcac caaaatcacg gattgtccaa caaatgacca atggtgtctt tgttcgaatg
    14041 gcaatcttag aatccgtact agcgagtaga aacgccaact aaaacacaag acgttaaaag
    14101 acgccagtga gcgtccacga gaggtgaaaa tatgacaaaa agaattctag tattagaaga
    14161 tggcacagtt tttgaaggca aggccttcgg agcagatatt gatgtaacag gcgaaatcgt
    14221 ctttaataca gggatgaccg gctaccaaga atccattaca gaccagtctt ataatggaca
    14281 aatcttgacc tttacttatc ctttggtagg aaattatggt attaatcgtg atgattatga
    14341 atccattatt ccaacttgta agggagtcgt tgtttttgaa gaagcgcgta gagctagcaa
    14401 ctggcgcaac caaatgacct tggatgaatt tttgaaagcc aagaaaattc cagggatttc
    14461 aggaattgat acgcgtgctc ttaccaagat tatccgtaag catggtacta tgcgtgcaac
    14521 cttgacccat gttggggaca gtatggacca tgtgacggac cagctccaag caacagtttt
    14581 accgacagac aatatcaaac aggtttctac taaaacgtct tatccagctc caggagttgg
    14641 tttgagcgtg gtgctagtgg actttggtct caagcactca atcctacgtg aactttctaa
    14701 acgcaactgt aacgtgacag ttgttcctta ttcgacaacg gctgaagaaa ttctccatct
    14761 caatcctgac ggagttatgt tgtcaaatgg tccaggtaac ccagaagacg ttccacaagc
    14821 actcgacatg attcgcggtg tacaaggaaa aattccaatc tttggtattt gtatgggaca
    14881 ccaactcttt gcaatggcaa acggggctaa gacctataag atgaagtttg gccaccgtgg
    14941 attcaaccat gcggtacgtg aaatcgcaac aggacgagta gacttcacca gccagaacca
    15001 tggttatgca gtcagccgcg aggatttgcc agagcacttg attattaccc acgaagaaat
    15061 caatgacaag tcagtggaag gtgtgcgcca cagataccaa cctgctttct ctgttcaata
    15121 ccacccagat gcagctcctg ggccacacga cgctagctac ctatttgacg agtttatcga
    15181 gatgatggaa gtttttaaac aatcaaacta agaacgagta aaagctcgtc ggagaattta
    15241 tgtaactgaa cacggacgga aagctcagaa tgtagagaaa ccaacttgga ttgtcaatcc
    15301 ttccttgatt tcctaaaatt caatcgcttt ctattcgtcc tttgtatctt atttaatgtc
    15361 gccctgagag gcgacgaata gaaaggagaa gggtgctccg tatttgctga actgaatacg
    15421 ggctacggac ggtgctaaaa agatagttat tcctagaact gacagttctt cgtcataact
    15481 cctattttag ctttgtccgc ttgacaccct taatatctta tatatgccta aacgtactga
    15541 tattcaaaaa attatggtga ttggttctgg tccgattatt attggtcagg ctgctgagtt
    15601 tgactacgct gggactcagg cttgcttgtc gttgaaagag gaaggttatg aggttgtttt
    15661 ggttaactca aatcctgcaa ccatcatgac ggacaaggaa attgctgaca aggtttacat
    15721 tgaaccgatt acacttgagt ttgtgacacg tattcttcgt aaggaaggtc cagatgcctt
    15781 gctaccaaca ctcggtggtc agacaggtct caacatggcc atggaattat ctaaaaatgg
    15841 tatcctagac gagcttggcg ttgaacttct gggtaccaaa ttatctgcca ttgaccaagc
    15901 ggaggaccgt gacctcttta aacaattgat ggaagagctt gaacaaccca ttccagaatc
    15961 tgaaattgtt aacacagtag aagaagcagt tgcctttgca gcgacaattg gctacccagt
    16021 catcgttcgt ccagccttta cccttggtgg tactggtggt ggtatgtgtg ccaacgagaa
    16081 agaattgcgt gaaatcactg aaaatggttt gaaattgtca cctgttaccc aatgtttgat
    16141 tgagcgttcg attgccggtt tcaaggaaat tgaatacgaa gtgatgcgtg actcagctga
    16201 caatgccttg gttgtttgta acatggaaaa ctttgaccca gttgggattc acacagggga
    16261 ttccattgta tttgcccctg cgcaaaccat gtcagactat gaaaaccaaa tgcttcgtga
    16321 tgcgagcttg agcattattc gtgccctcaa gattgaaggt ggatgtaatg ttcagctagc
    16381 tcttgatcct aacagcttca agtattatgt tatcgaagta aaccctcgtg tatcacgttc
    16441 gtcagccctt gcttctaaag cgacaggata tccgattgct aagttggctg ccaagattgc
    16501 agtaggtttg accttggatg aggttataaa cccagttaca ggttcaacct atgccatgtt
    16561 tgaaccagcc cttgactacg tagttgctaa gattccacgt ttcccatttg acaagtttga
    16621 aaagggtgaa cgccgtctcg gtacacaaat gaaggcgact ggagaagtca tggcaatcgg
    16681 tcgtaacatc gaggaatctc ttctcaaggc ctgccgctcc cttgaaattg gggtgcacca
    16741 caatgaaata cctgaacttg cagcagtttc tgatgatgcc ttgattgaaa aggttgtcaa
    16801 agcccaagat gaccgtctct tctacgtatc agaagccatt cgtcgtggct acacacctga
    16861 agaaattgca gagcttacaa aaattgatat cttctatctt gataaactct tgcatatctt
    16921 tgaaattgag caagaattgg gtgctcatcc acaagattta gaagttttga aaacagccaa
    16981 acttaatggt ttttcagacc gtaagattgc tgaactctgg ggaacgactg atgataaagt
    17041 tcgccaactt cgcttggaaa acaagattgt tccagtttat aagatggtag acacttgtgc
    17101 ggcagagttc gactccgaaa cgccatattt ctattcaacc tatggttggg aaaatgaatc
    17161 tatcagatct gataaagaat ctgtacttgt cctaggttca ggtccaatcc gtatcggtca
    17221 aggggttgag tttgactacg caaccgttca ctctgttaag gctatccagg cagctggtta
    17281 tgaagctatc atcatgaact caaacccaga gaccgtttca acagacttct ctgtatcaga
    17341 taagctttac tttgagccat tgacattcga agatgttatg aacgtcattg acttggaaca
    17401 accaaaggga gttatcgttc agttcggtgg tcaaacagcc atcaaccttg cggagccatt
    17461 ggcaaaagca ggtgtgacca tccttggtac acaggttgct gacctagatc gtgccgaaga
    17521 ccgtgacctc ttcgagcaag ctctcaaaga attggatatt ccacagccac caggacaaac
    17581 ggctaccaat gaagaagaag cagctcttgc agctcgcaag attggcttcc cagttcttgt
    17641 ccgcccatct tatgtacttg gtggacgtgc catggaaatc gttgaaaacg aagaagacct
    17701 tcgttcttac atgcgaactg ctgttaaggc tagtccagac cacccagttc ttgtcgactc
    17761 ttatatcgtt gggcaagagt gcgaagttga tgccatttca gatggtaaaa atgtactcat
    17821 tcctggtatc atggagcata tcgaacgtgc tggtgtccac tcaggtgact caatggctgt
    17881 ttacccacca caaaccttgt cgcaaaaggt tcaggagacc atcgcagact acaccaaacg
    17941 cttagcaatc ggtcttcact gccttggaat gatgaacatc cagtttgtta tcaaggatga
    18001 aaaagtctac gttattgagg tcaatccacg tgccagccgt actgttccat tcctatctaa
    18061 ggtaaccaat attcctatgg ctcaagtagc gaccaaactc attcttggtc aaagtttatc
    18121 agaacttggc taccaaaatg gactttaccc tgaaagcacc cgtgttcata tcaaggcacc
    18181 tgtcttctcc tttaccaaac tagctaaggt agacagcttg ctcggtcctg aaatgaagtc
    18241 aacaggtgaa gttatgggtt ctgatgctac tttggaaaaa gctctctata aagcctttga
    18301 agcttcttat ctacacttgc caacttttgg taatgtcgtc tttaccatcg ctgatgatgc
    18361 caaagaagaa gccttgaact tggctcgtcg tttccaaaac attggttatg gaatccttgc
    18421 gacagaaggg acagcagcct tctttgctag tcatggcttg caagcccaac ctgttggtaa
    18481 gattggtgat gacgataagg atatcccaag ttttgtacgc aaaggaagaa ttcaagctat
    18541 cattaacaca gtaggaacaa aacgaactgc tgacgaagat ggtgagcaaa ttcgtcgttc
    18601 agccattgaa cacggggtgc ccctcttcac agcgctagat acagctaatg ccatgcttaa
    18661 ggtacttgaa agtcgtagct ttgttacaga agcgatttaa atagaaaaat aagat
//
LOCUS       DAVKNL010000041        17741 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVKNL010000041 DAVKNL010000000
VERSION     DAVKNL010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17741
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.041"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            21..149
                     /gene="blpC"
                     /locus_tag="VW411_001427"
     CDS             21..149
                     /gene="blpC"
                     /locus_tag="VW411_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEU9794146.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDLLYNINRYAHYIT"
     gene            complement(220..1560)
                     /locus_tag="VW411_001428"
     CDS             complement(220..1560)
                     /locus_tag="VW411_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9794147.1"
                     /translation="MNIAWILLYTLVTNGLEIVIFFKVDGIDLTFERIFKAFLLKILL
                     AFVFVMISYIVGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRKEILDKAFQK
                     SLTQINWIMGGYYLVMESLSFFEYEQSIQSKTVRHLILVFYLLFFMGVIKKLDTYLKE
                     KLYERLEQEQALRYRDMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EVYGSVLKDSSQKLQNNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTIVSILCDNAIEASVEASQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQNQVFRQVLTVIHTE"
     gene            complement(1574..2311)
                     /locus_tag="VW411_001429"
     CDS             complement(1574..2311)
                     /locus_tag="VW411_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9794148.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRAHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINKLH"
     gene            complement(2316..2654)
                     /locus_tag="VW411_001430"
     CDS             complement(2316..2654)
                     /locus_tag="VW411_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9794149.1"
                     /translation="MKYMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            2930..3259
                     /locus_tag="VW411_001431"
     CDS             2930..3259
                     /locus_tag="VW411_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEU9794150.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(3427..4476)
                     /locus_tag="VW411_001432"
     CDS             complement(3427..4476)
                     /locus_tag="VW411_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9794151.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGTATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFCQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSLLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(4473..5204)
                     /locus_tag="VW411_001433"
     CDS             complement(4473..5204)
                     /locus_tag="VW411_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9794152.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRSKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            5272..5682
                     /locus_tag="VW411_001434"
     CDS             5272..5682
                     /locus_tag="VW411_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU9794153.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            5692..5979
                     /locus_tag="VW411_001435"
     CDS             5692..5979
                     /locus_tag="VW411_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794154.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIINQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            complement(6669..7805)
                     /gene="dnaJ"
                     /locus_tag="VW411_001436"
     CDS             complement(6669..7805)
                     /gene="dnaJ"
                     /locus_tag="VW411_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU9794155.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(8564..10387)
                     /gene="dnaK"
                     /locus_tag="VW411_001437"
     CDS             complement(8564..10387)
                     /gene="dnaK"
                     /locus_tag="VW411_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEU9794156.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKTKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(10876..11400)
                     /gene="grpE"
                     /locus_tag="VW411_001438"
     CDS             complement(10876..11400)
                     /gene="grpE"
                     /locus_tag="VW411_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU9794157.1"
                     /translation="MAQDIKNEEVEEVQEEEVVKTAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(11427..12461)
                     /gene="hrcA"
                     /locus_tag="VW411_001439"
     CDS             complement(11427..12461)
                     /gene="hrcA"
                     /locus_tag="VW411_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEU9794158.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVHLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(12625..13135)
                     /locus_tag="VW411_001440"
                     /pseudo
     CDS             complement(12625..13135)
                     /locus_tag="VW411_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
     gene            13743..16076
                     /locus_tag="VW411_001441"
     CDS             13743..16076
                     /locus_tag="VW411_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU9794159.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFRPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKVIDYFSSATQIGMTATLKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDANGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGDPVKVLE
                     TGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            16089..17552
                     /locus_tag="VW411_001442"
     CDS             16089..17552
                     /locus_tag="VW411_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU9794160.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSNM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLDPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCDFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            17552..>17741
                     /locus_tag="VW411_001443"
     CDS             17552..>17741
                     /locus_tag="VW411_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HEU9794161.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDD"
BASE COUNT         5587 a         3614 c         3381 g         5159 t
ORIGIN      
        1 tcaaaaagga gaatcataac atggataaga aacaaaacct aacttcattt caagaactaa
       61 caactaccga actcaatcaa attacaggtg gaggattgtg ggaagattta ttatataaca
      121 ttaatagata tgctcattac atcacataag aacttcatca tccaatacaa ctataaaaaa
      181 ataagaccga gaaacaagta ctctcggtct tatttttcat cattctgtat gtatcacagt
      241 aagtacctga cgaaagactt gattttgaca ggtggtattt agactggtat taggatggct
      301 ttccacaatc ttcatgacgg tatagagacc aactcctctc tcctcccctt tagaactggc
      361 tccaaaggag aagatttcag aaatatcgat gccctcttct ttgatggagt tttcgatgat
      421 aaaggtctcc tgtgctccat tttttaaaaa ggcgattgaa acatgaggtt gactagcttc
      481 cacactggct tcaatagcat tgtcacaaag gatagacaca atggttagaa aatcaagtag
      541 actcatcccc tcgacctgaa tctcctcagg aacttcgaca ttaaagacaa tgttcttatc
      601 tctggctttt aaaaatttcc ctgctagaag acttttgagg gctttatcac gaatatttac
      661 caatctgccc aggtcatatt tattgttctg caatttctga ctggaatcct ttaagacgga
      721 gccatagacc tcttttatct gctccatatc ctcctcttca atgcccagac gtaagctagt
      781 caagaggttg gtataatcat gacgaaagct ccgtacttcc ttgtaaagct cctctatatg
      841 ccgactatag cgttccatat ctctatagcg cagggcctgc tcttgttcca atctctcata
      901 gagtttttcc ttcaaatagg tatccaattt cttgataacc cccataaaaa agagtaggta
      961 aaagactagg atgagatggc gaacagtctt tgattgaata ctttgttcat attcaaaaaa
     1021 agacagactt tccatgacta gatagtagcc acccattatc cagttaatct gagtcaggga
     1081 cttttgaaag gctttatcga gaatctcctt tctcaagcta gtaaaatcgt agtccaacca
     1141 tttcaaaaaa gctagagaaa tgaagaaatt gaaaattatt atacataacc cagtaaatga
     1201 gtagccatca tatacttgcc cttgtcccaa aaatggaagc acaaaatagg agactcctct
     1261 ataaaagaga ttcaccaata tcattggaaa gagaccataa aagaaaagga gttttttagg
     1321 aagccctctc aataataaga aagataagcc tatgccgtac aagggttcca taaaataaga
     1381 taggtaaaca tttcctacta tatagctaat catcacaaaa acaaaggcca acagtatctt
     1441 caaaagaaag gccttaaaaa tcctctcgaa agtaagatca attccatcca ccttaaagaa
     1501 gatgacaatt tctagtccat tagtaacaag tgtatacaac aatatccaag caatgttcat
     1561 aaattctcct agctcagtgt aatttattga tggcctcaga cacttccctg accttataac
     1621 gggcgattag acaacttcca ccattgggaa agaaaagcag tttttctttc ttatccaaat
     1681 gcaccacatt tgcaggattg atgagaaaag agcggtggca ctgcaagaga cggggctcct
     1741 gcttgaaaac ctcctctaaa ctcgctgtaa attccagcct gtctgtcttg gtatagagaa
     1801 taacacgatg ggctctgggc gacgtttcga gatagtaaac ctctttaaaa ggatactgaa
     1861 attgggcaaa ttttgattta aagtaaaagc aatcttccgc cagactttta ctatcttgac
     1921 tattggcata gaggagggct gtctcgatcc gagattcaaa ctcctctgct gacaaggcct
     1981 tatcaatgta gtccaaagca gacacttggt agcgaaaaga caggggcata aactccgagt
     2041 gagtcgtcac aaagacaatc agggcataag gatcccgatc ccgaatcttt ctagccactt
     2101 ccagtccctt catctcttca tttcgaatct caatatccaa aaagaatagc tgatgggccc
     2161 ccttctcatg cacttcagcc agcagttggt ccggcttgcc aaatacctca aaagagctag
     2221 gaatgatatg atgtgctttc aaaagtttct caatcgtcgt ttcaattcta gtctgttggg
     2281 aaaaatcatc ctctaaaaca aatattctca tcttcttact ctccctcttt caaccatttt
     2341 tgacgaattt ctctatagcg acgtctggca tacttgacag cgtattgacc ttcttctccg
     2401 agaaaaagga ttctttcttg aaaatcaatc gattttaatt tatcaagatt aaccaaacaa
     2461 tttcgatgac atctcatcaa ggtttcccca cattggttct caagattact taaattttga
     2521 agcatattaa aactagcttc ttctgtaaca atctgtacgg tatgggcttt agttggatgt
     2581 gtttggatat agtagatatc gtcgatgttt actttataga ctgttttctg cgtctgaata
     2641 atcatatatt tcattggatt ctcctcctaa gaaaagaata acagaaaagg tctagcccaa
     2701 gcacacccaa tcctaaacag tctttgaaaa ctcctaaaag gattattttc tacaacatca
     2761 tgatgagctt acaactattt tatcataaaa ttcactcagc gcaattcaag acatttcaat
     2821 gacaattaaa gatttgaata aaaaaaaaga attaaaagtg ataaactaac agtatcaaaa
     2881 cacagttgcc agaagtaaga ctggcaccca gattaaagga ggcattccca tgacagatac
     2941 agaccccatc aaaagagctc atactttgat tactgactta aacaaagcct accaagcatg
     3001 caaacaggca agcgctgacg acgtccgctt tcaggagcaa ttaaactcta ttcttggttt
     3061 tctagccaag gctgaaacag tggataatcg attcttgatt gaactagaaa aattttacca
     3121 gacttccagt cttctcatgg gactcagcgc tcttgatcca gatgctccaa ctcgcgccgc
     3181 ttggcgggcc tatgaccgct tccactttga ccaagtcaag accaagttaa tactcaatga
     3241 aaatcaaaga gcaaactagg aagctagccg caagctgtac ttgagtacgg taagacgacg
     3301 ctgacgtggt ttgaagagat tttcgaagag tataagtctc tacggaccaa ccattatcct
     3361 gtagtaacaa aaaagaagtt gagacagact gaactcaact tcttttttag tgtcgtatca
     3421 gcaatgttag tcctgcatct gacgttttac ctgataaggc aaatacaaga ctagtaaaac
     3481 caaaccagct cctagtaagg ctagaagggc tagtttttct tggaaggtaa tcaacccaac
     3541 aactaattcc acaagtaaaa caaaactggt caatcctcga actacctcct gtaagccttt
     3601 ttccttttgc cccttgtcca gcggaaagag ttgggtcaaa tactggtagt caaaggcatg
     3661 atagagggcc agcaactgga agagcaagag gtagttaaag agaactacca ctgctgtcgc
     3721 aatccaagct tgctcgataa aaacctgcgc cagcaaggaa agcaagagaa gacgaagact
     3781 gagagcaaag aggtcgccat ttcgcagata agaacgcaga tagagatttt gccaaatctt
     3841 cccaggcacc ttctgaacag cctttaaaat aaagtccaga taggcacgac gcttaacgct
     3901 gtttgaaatt cccttgacct gcgtaaagag ggcaaagaaa cgaagcaaga cttgcttacg
     3961 cttgctttct tgagaaataa catagtccca gtccagtcca gtttcagtga aaaatttgct
     4021 ggccttttga caaaagtgga aatattttcc tacccccaat aaaagcacat agagcagaaa
     4081 aactggcaag ccataaccca ttgctaaaaa taaaggcgca aataacagca agaaaagggt
     4141 ctgtacaaag agccaaaaga ctagggaaat gccagtttga cgcttgagat ggagcttaat
     4201 ttcctcttct ccaactaaga gaaagagctt gtctggagcc tccatatagg tggcagttcc
     4261 tccccaaagt aaaagtaaaa cagacgtaat tcctacaaac aaaaggatag gccaatgatt
     4321 ttcaggaaaa tgttgtaaga gttgactgta ctggtaggct agaaagccca acaggacaag
     4381 caggaacaag acaaagtggt cattgagcac atagcgcaga taaccaagac actccttacg
     4441 aaaggcctgc tttctcttta aaaacaagtc tttcatagat cctcctcttt ggtcagagcc
     4501 aagtaaatat cattcaaact agcctcaggc atatcaaagg cttcacgtag ttgcaggaga
     4561 ttgcctttgg aacgcacctc tcccttgtga agaatgacaa aggcatcaca catcttctcc
     4621 gccgaatcca gcacgtgggt actcatgaga atagacttgc ccttttgctt ctccacttcc
     4681 aaaagctgaa tcaaatcaga aatagccagc ggatcaagac caaggaaagg ctcatccacg
     4741 atgaaaagac ttggatccac cacaaaagca cagataatca tgaccttctg cttcatccct
     4801 tttgaaaaat gaacagggaa ccagtctaat ttctgttcca aacggaacat ttttaacaag
     4861 ggctctactc gttcgaaagc cactttttgc tcaataccgt aagccatagc aaccgtttcg
     4921 atatgctctc tgagggtcaa ttcctcatac agactaggcg tctcaggaat gtagccaatc
     4981 tgcttgcggt agctagtcgc atctccttgc agagtcaggc cattgatatt gatggagcca
     5041 ctataaggtg ccaacagacc gataatctca ttgatcgtcg ttgatttccc agcaccattg
     5101 agaccaatca aaccgaccaa ctgcccactt tcaacagtaa aggacacatc tttcaaaaca
     5161 ggaacatgaa catagccacc tgtcaggttt ttaatttcta acatattttc tccaaatctg
     5221 gtataatgta gctatattat atcaaaattc agtacagtag aggtagattt tatgtcagat
     5281 tgcatttttt gtaaaatcat cgcaggggaa attcctgctt caaaagtata tgaagatgag
     5341 caggtccttg cctttcttga tatctctcaa gtaacactag gacacacctt ggtcgtgcca
     5401 aaagaacact atcgcaatct tttggagatg gatgctacga gcgccagcca actctttgcc
     5461 caagtaccaa aagtagctca aaaagtcatg aaagtcacta aggctgctgg tatgaatatc
     5521 atttccaact gtgaagaagt cgctggtcaa acagtttttc atactcacgt tcaccttgtg
     5581 cctcgctaca gtgctgacga tgacctcaag attgatttta tcgcccacga accagacttt
     5641 gacaaacttg ctcaagtcgc tgaaaccatc aaaaacgctt aaggagttgc tatgaaatta
     5701 tccaacctac tgctatttgc aggagctgca gccggaagtt atctggttac aaaaaatcgc
     5761 caaaccatca cagatgaagt cttgaatacc actgaccgcg ttcaagctat caaggacgat
     5821 gtggatatta tccaaaacag cctgcaaatc attaaccagc aaaaagaact tatcaaggaa
     5881 taccaagaag acttgactta caagtttaag gtcttggaaa aggatatcca gactagacta
     5941 gctgtgataa aagaaatgca gggaactgaa gataagtaaa aagagcccga cggctctttt
     6001 ttaaaacgta aacaagttat agtgaattga atttagaata gcacaccata gtttctaaag
     6061 cattgttaga gattacttta aaatcctctt atcaatttgt tcatattcta tttcaatcta
     6121 ttatatatat cccatcaact atgcatcata atttaggtaa ttcatactca ataaaaatca
     6181 aaaagcaaac tagaaagcta ggcacagact gctcaaaaca ccgttttaag gttgtggata
     6241 gaactgacga agtcagctca aacactgttt tgaggttgtg gatagaactg acgaagtcag
     6301 taaccatacc catgacaagc cgacgctgac gtggtttgaa gaaatttttg aagagtatca
     6361 ttcaccattt cacaactaaa tcgaagactt tctgccgtaa tgaaaacacc tgaaacagct
     6421 tggtttcagc tgtccggaaa ctttaaaact taggttcaaa gtttaggtat ggaacttcga
     6481 aggaggtcac taccgtccgt cattacttag gaaagtctta aaaaatctat aaagtaaaaa
     6541 gcaaactagg aaactagcca cgggctgctc aaaacactgt tttgaggttg tagatacgac
     6601 tgacgaagtc agtcacatat atacggcaag gcaacgctga cgtggtttga agagattttc
     6661 gaagagtatt attctccatc aaaggcatct ttaatatggt caaagaagcc ttttttcttt
     6721 ggatttactt tcaagtcacc agcagccgcg aattctttca aggctacttt ttggcggtcg
     6781 ttcaagcctg tcggtgttac gacattaaca gtaacgtatt ggtcaccaac tgcaccgcca
     6841 cgaaggctcg gtgccccctt actacgtagg cggaacttct taccagtctg agttccctct
     6901 ggaataacca attcaacatc accgtgaaca gttggaatat ctactgtatc accaagagcc
     6961 gcttggacaa agttgaggtt gagattgtag aagatagtcg ttccttcacg ttcaaacttg
     7021 tcgctagctt ccacagaaac tactacatac aagtcaccat aaggtccacc gttaaagcct
     7081 gcttcacctt gaccagcgag gcgaatttgt tgacctgttt ccacaccagc agggattttc
     7141 acatgtacgc tatgagcttg tttctcatga cctgttccat gacaggttgt acatggatat
     7201 ttgatttctt ttcctcgacc gtgacagaca tcacaggtta cttggcgacg catcatacca
     7261 agaggagtct gcgtatcgac gttaatgaca ccagcgccat gacagcgtcc acaagtgact
     7321 ggacttgtcc ctggcttagc accagatcca ttacatgtac gacagccagc ttcacgatga
     7381 tacttaactt ccttctcagt tccgaagata gcttcttcaa aggtcaaatt gacacgatac
     7441 tggagatcat ctccttggcg aggagcgttt ggattgcgcg aagaaccgcc tccgccgaag
     7501 aaacttgaga aaatatcctc aaaaccaccg aagccacctg ccccattgaa accgccgaaa
     7561 ccaccagctc caccaaaacc accattggcg cctgcagcac catactggtc ataggcagca
     7621 cgtttttggt cgtcactcaa agtctcatag gcttcttgaa cttccttgta cttgtcctca
     7681 gcaccaggct ccttgttgat atctgggtga tattttttgg aaagcttacg ataagccttt
     7741 ttgatttcgt ctgccgaagc gtttttggat acccccagac gatcataaaa ttcagtattg
     7801 ttcataattc aagatacaaa gggcgtcaaa cggacacagc catctaggag ttttgacgac
     7861 ggacgcttac gtcctagaaa aaactatctt tttggcacag tccatagccc gtgttcagtt
     7921 acgaacaccc ttattccttt ctattgatgt tctgaatcga accaccattt aggtcgggtt
     7981 caattccttt cttttatatt tagtaagaaa ttctgaaacc cgtgttcagt tacgaacact
     8041 ctttgttcct ttctattgat gagacaaaac ccaaacaaat gagattccgt ccctttttat
     8101 cgaaaaacag aggctgggtt gcaacctctg caaataagta aattaacttt attttgattt
     8161 taaatatcct ataagtacag aaatatttat atatttttgt ctcttcttag tatttgccta
     8221 tataattcca attgatcata agaaatacaa ctaccgtcca aatcatacca attatgaacg
     8281 gtattacaaa tttatatgtt tgtatttcta atgatatcat agctggtacc atcgccgata
     8341 caaatgcaga caacaatact gaggtaacaa aattccttac cgcttcatca tttttgatac
     8401 aactatcatg ttcagctaat ataattctct atatctagct aaatattcta ttggaatatt
     8461 atcaataaaa tcttttgatg ttctatagtt atcagcttga ttacaaaaat cattataaac
     8521 ttttcgtgta ttttttagat actcttcatc caatacactc atcttacttt tccgtaaact
     8581 ctccgtctac gacgtcatcg cctgcgttcc ctgttgcttg tgcgccttct gctccttctt
     8641 gagcttgttg cgctgctgcg gcttgttcgt agagtttaac agcaagtcct tgagcttttt
     8701 cgttcaatgc ttcaagtttt gttttcatgt cgtccaagtt gttgtcttct tgagctttct
     8761 taaggtcatc aagggcagct tgggcagcgt cacgttctgc gtcgaagcct ttaccttcag
     8821 tttccttgat tgtcttttca gtcgcaaaga ttgcttggtc tacttcatta cgaaggtcta
     8881 cttcttcttt acgtttctta tcggcttcag cgtttgcttc tgcatctttc atcatgcggt
     8941 cgatttcttc gtcagtcaaa cctgagttcg attggatgac aatagtttgt tctttttgag
     9001 ttccaaggtc tttggcctta acagacacga taccgttctt gtcgatgtca aatgttactt
     9061 cgatttgagg aattccacga ggtgcagctg ggatatcagt caattggaag cgtccaagag
     9121 tcttgttatc tgctgccatt gggcgttcac cttgaagaac gtggatatca acggctggtt
     9181 ggttgtctgc tgctgttgag aagacttgtg atttagatgt tgggattgta gtgttgcgat
     9241 cgataagttt tgtaaatact ccacccattg tttcgatacc aagtgacaat ggcgttacat
     9301 caagaaggac aacgtccttg acatcaccag taatcacacc accttggata gccgcaccca
     9361 tagcaactac ttcatcaggg tttactgatt tgtttggttc tttaccagtt tcagctttaa
     9421 cagcttcaac aacggcaggg atacgagttg aaccaccaac aaggataact tcgtcgattt
     9481 ctgacaagct caaacctgca tctgaaaggg cttgacgaac tggaactttt gtacgttcaa
     9541 caaggtcacg agtcaaatcg tcaaatttcg cacgagtcaa agtcatttcc aagtgaagag
     9601 gtccagcctc acctgcagtg ataaatggca agctgatttg tgttgaagtt acaccagaaa
     9661 ggtctttctt cgctttttca gccgcatctt tcaaacgttg cattgccatc ttgtcagtag
     9721 acaagtcgat accgttttct ttcttgaatt ctgctaccaa gtggtcaatg attttttggt
     9781 caaagtcgtc accaccaagt ttgttgtccc ctgcagttga caatacgtcg aagacaccgt
     9841 cacccaattc aaggatagag acgtcgaatg taccaccacc aaggtcaaat accaagattt
     9901 tttcttcttt gtcagtcttg tccaaaccat aagcaagagc tgctgcagtt ggttcgttaa
     9961 caatacgttc tacttcaaga ccagcaattt taccagcgtc ttttgttgct tgacgttgag
    10021 cgtcgttgaa gtaagccgga actgtgataa cagctttggt tactttctca ccaaggtagt
    10081 cttcagcgta gcctttcaag tattgaagga tcatagctga gatttcttgt ggagtgtatt
    10141 cttttccatt tgcagaaact ttttcagaag ttcccatctt agatttgata gagataactg
    10201 tatctgggtt tgtaactgct tgacgttttg cagcatcacc aacgatgatt tctccgtttt
    10261 tgaatgagac tacagatgga gttgtgcggt ttccttctgg gtttgcgatg attttgcttt
    10321 cagttccttc aagaactgca actgctgagt ttgttgtacc taagtcaata ccgataattt
    10381 tagacatgtg tttttctcct tgttagttaa ttttctattt ttctttttga tactcttctt
    10441 aagtggcgac agtggcgacg ccgtcagagc ttgacttcgt caatctcttt gactaaactt
    10501 tgagcctaag gtctcaaaat ttgcgcaaat agcgccacgg cgaagagtat cgtgtttggt
    10561 tcgcttcgct cactattaca aagccaaaca tattttatct ttcttcatag tttattgtcg
    10621 tttcggacaa gttttctatt atgtaaattg cgacacgagg agtcgaaatc gattttattt
    10681 cgacgacgag ttagtaagga agctaggcaa acgccatagc gattgccgtt ttctgacgag
    10741 ttgctttagc taccgtcaga attgccgaat cgaacacgcc ctaagctctg agtaaaaaag
    10801 atagattttc ttgtgttcat cgaacacttc gtcaatctcc tatttttact gcgagctttt
    10861 tacgggcttt atatcttagt tatacaccac taccattgct gggcgtagga tgcggtcatg
    10921 gagtttgtag cctttttgaa agacttgagc gatggtatct actgggtgtt catcgtctgc
    10981 tgggagagtt tggatggcca tatggtagtt atggtcaaat tcgccatctg ctgcgatttc
    11041 ttcaattcct tcttctttca aagcgtgaat caagctttct tgcaccatcc ccaagccctt
    11101 cttcacatca tctgtcaaac cttcaactgc aagtgcacgc tcaaggttgt caagagatgg
    11161 taagattgct tttgccaagt cctggctacg ataacgttgc aagttttgac gttcttcatt
    11221 ggcacggcgt tggatatttt gcatttctgc atgagcgcga agatatttgt tttcgaactc
    11281 atctgcacgt tcatttgcca agtccaactc agacttttca ggagttgttt cttcagctgt
    11341 tttcacaact tcctcttctt gaacttcttc tacttcttca ttttttatat cttgggccat
    11401 gttcgcctcc tttaatgatt tcaatcttaa tgtacttcgt aatgattact gctgaggtag
    11461 cggtaaaaat ctgtcaactt catggtcaaa acacggttga ccacattgac ttgattgatt
    11521 agctgttggt aatccagatt aactggaccg ataatggcta gaattccaac tccccgataa
    11581 ggaatgagga acttactact gattaccgct aggtcagcta aacaggactc ttgaccgtct
    11641 gcaacacgaa cattttgcat ctgatcctca cgcaacccct cacgaatctc caaggccacc
    11701 ttttgcggtt ggtcaaagaa ctgataggct gctagattgg caaaattcaa gagatggacc
    11761 ttgcccgcca tcacaatgtt ttcgttgaac atttccttaa agatgtgttc aaagagatcg
    11821 atgacattat ccgttgttgt aaagtaacgc tggataatct gcggaatctc cgtccgaatc
    11881 ttgtagtgaa tatctaaaac ggtgtgaccg aggaaacgtt cctgaatgat gctcttcagt
    11941 ttcagcaaat cctcctgcaa gaagttcctt ggaatcagaa actgactagt aaccgttcgc
    12001 gactcgtcta gggtaaatac cgccaaggct gtatgttgcc ccaaaacaac gatatcaaag
    12061 gctgtcaaac gttgcctgct cggctcaaca tccagtgcca ctaccgtaca gccactcagg
    12121 tctgttagta agttagcagc ctcttgcaga atatcctcca atttgaagaa ttcctgatca
    12181 aaggctttga caatctcata tacctcattt tcagccagcc ggtcaaaatc cagtgagtgt
    12241 ttcacatagt actgaaaacc agcaacactt ggcatccgac cacttgaagt atgagccttc
    12301 tcaagcaacc cttgtttttc tagttccgcc atgtcattac gaatggttgc actgctagag
    12361 ttaatagact cttgcaaggc ttttgatccg acaggttcgt gcgttttggt aaagatgtca
    12421 ataatcagat ttaaaatatc ctgctgacgc tctgtaacca tctcatcact cctctctgtc
    12481 tgatcaatta gcactcaatg catccaagtg ctaactcatg attctattat acacccttag
    12541 aagagaatgt caagacaaaa actcaaaaaa ttagcactct tttatcaaga gtgctaaaaa
    12601 tcaatctgaa gacctagaac cttgctattc atctactcgg tatttccttt ttagtctgaa
    12661 ataaccataa attagataag aaatcagaaa agcatataaa acaaaaataa gaaagaaaga
    12721 catagaaaga tcctctctaa acaaactcat acaaacaaca agaccacctg agaaagtagc
    12781 tgtacaggaa cgaagtctag atcgcataac ttcccatttg agccctttac tcatatagac
    12841 ttgatcctct ggaagtaaat cagaagacga tttacgaaga atcgaacaag aacctgctcc
    12901 tatcaattcc caacctctat ctctaaaatc ttgcagttca tgcttatact tttttaagaa
    12961 atctagaatc atagatacgg tagatgacat cgtctggttg acattggtca aaatagaaca
    13021 aaccaaaacg actcgttcta tacctccaac ctttcaaatg catctcatgt aaatgttctt
    13081 cttccttgtc caaatcaaca atggtgaaaa tccgaaattc tactctgcta ttcattgtct
    13141 taccccaaaa ttagaaaaca tgcctggcgt tatttattag ataattcttt ccacttttga
    13201 ctcaatctcc aaaaaatata agaaatctga atcgcaaaaa ctatcaataa aacccaatct
    13261 attatgaaaa tcaaaaacac tttccaactg aaagaactac ctccagtgac aaactttgag
    13321 aaaaacggta gtagagctaa aaagagaaat aaaataggaa gcatccgcat tgttaaaatc
    13381 cgtttggcat aaaaaaatct ttatttaaac gaaaatatta tggcaaaatt tacgccagtt
    13441 tttgaacggc tgatgtagat attttatact ttcaaaatgt ttaaatgtga ttatttattt
    13501 ttgaaaaata gatcaccagc ccgactgaaa gtgcttatag aatgataata agtcgcctgc
    13561 cgaaaacagc gaaaaatagc ggtgttatgc ggagataatc tgacgcgatg cgaaagtata
    13621 ttgcatactt attttcaaca atttagcaga gtatttttat aagtgtgata taatagaagt
    13681 ataatttgtt ctgatagttt attttatgga gaagtagatt tttagaatgc ggagggttca
    13741 atatggttga gtttataaag tctaagaaag aaatgagtga ggaggatatt aaagcaaatt
    13801 tcatcactcc tgctattgta tccaaaggat ggaaaaatgg tgagcatatc gcttacgaag
    13861 aatacttcac tgatggtcga attgaagtta gaggagataa ggctcgtcgt aaagaaggaa
    13921 aaaaatcaga ctattcactg tattaccaat ttggaactcg aattgcaatt gttgaggcaa
    13981 aggataataa acacagcgtt cgagcaggat tacaacaagc tattgaatat ggagagattt
    14041 tagatgttcc atttgtttat tcttcgaatg gtgatggctt tattgaacac gaccgtatca
    14101 cgagagaaga acgtgagctg gagttagacg aattccctac tcgtgaagaa ttattttctc
    14161 gtatgacgaa ggaaaaagga ttgacgtacg aaattacaga agctatctca actccatact
    14221 atacagacgc cttctcaatg aaaacgccac gctattatca gcaaatagct atcaaccgta
    14281 ctattgaaac agttgccaga ggacaaaaac gagtaatgtt tgtgatggca acaggaacgg
    14341 ggaaaacgtt catggctttt caaattattc atcgccttcg aaaagctggt ttggctaaac
    14401 gagttttatt cttagcagat agaaacatct tagtagacca aacgatggct gaagacttta
    14461 ggccattcga aaaggtaatg acgaaaatta caccaaaact tttgactgct cctgaaaaat
    14521 taaattcttt tgaaatttat ctagggcttt atcagcaact aactggtgaa gatggaactg
    14581 aaacacatta tcaaaaattt gacaaagact tctttgattt aatcgtaatt gatgaagcgc
    14641 accgtggttc agctaaggaa aacagtaact ggcgtaaggt aattgattat ttcagttctg
    14701 cgacacagat tgggatgacc gctactctta aagaaaccaa gaatgcttcc aatacggaat
    14761 actttggtga gccaatctat acttatagtt taaaacaggg aatcgaggat ggttttttgg
    14821 ctccatatcg tgttatgagg gttaatttag atgtggatgt ggatggttat cgtccagaaa
    14881 ctggaaaagt tgatgctaac ggacaattaa tagaagatag gtactacggc aggaaagatt
    14941 ttgataaaac cattgtcatt gatgatagaa cgcaaagagt tgccaagttt gtttctgatt
    15001 atatgaagca aaacaatgca cgatttgata aaacaattgt tttttgtgtt gatattgacc
    15061 atgccgagcg aatgcgtgct gcacttgtaa aagagaatct agacttagtc caagaagact
    15121 atcgttatgt catgcaagta actggtgaca acgctgaagg aaaagctcaa ctggataact
    15181 ttatggatgt caattctaat tttcccgcta ttgtaacaac gtctaaatta ttaacgacag
    15241 gagttaatgc taaaacatgt cgtttgattg ttttagactc taatatccaa tccatgactg
    15301 aatttaaaca aattattggt cgtggcacac gtctttatcc tcaaaagggg aaagaatttt
    15361 ttacgattat tgattttcga aatgttacca atttgtttgc tgaccctgat tttgatggtg
    15421 atccagtgaa ggtgctagaa acaggtgcga aaacagtcag tggttctacg cccggtttcg
    15481 tagatgagga aggtgaccca gtagaaaaat atatcgttac agacaagcag gttaccattc
    15541 ttaattctac tgttcaagta ttggatgaaa acgggaaact gattaccgaa agcctgaccg
    15601 actacactcg aaagaatatc ttaggtagct acgccacttt gaacgatttt atcacagttt
    15661 ggcatacggc agataagaag aagcttatct tagacgaact ttataaaaaa ggagtttatc
    15721 tagatgctat tcgagagtcg gagggaatat cagaacaaga aatcgatgat tttgatttac
    15781 tcctaaaact tgcctatggt caaaaagaat taaccaaaac ggaacgtatc aataaactca
    15841 aacaaagcgg atatttatat aaatatagtg aggaagcgcg tgctgttttg gaaattttac
    15901 tgaacaaata catggataaa ggtattggag aactcgaaag cattgaaaca ttaaaacttc
    15961 cagaatttca gatatatggt ggaaccttca aaatcatcaa tacttatttt ggagataaaa
    16021 aacgatattt acaagcaatt aaagaattgg agcaagagct atttacagta gcttaatgaa
    16081 aggaaagtat gtcaattaca tcatttgtaa aaagaattca agatatcact cgaaacgatg
    16141 ctggtgttaa tggtgatgct caacgtattg agcaaatgtc ttggttatta ttcttaaaaa
    16201 tttatgatag ccgtgaaatg gtttgggaat tagaagaaga cgagtatgag tcaattatcc
    16261 cagaggaatt aaaatggcga aattgggctc atgctcaaaa tggggaacgg gtattgacag
    16321 gcgatgaatt acttgatttt gtcaataaca agttattcaa agagttgaaa gagcttgaaa
    16381 taacttcaaa tatgcctatt cgaaaaacga ttgttaaatc agcttttgaa gatgcgaaca
    16441 actatatgaa aaatggcgtc ttgttacgcc aagtcatcaa tgttattgat gaagttgatt
    16501 tcaatagccc tgaagatcgt cattcgttta atgatattta cgaaaaaatt cttaaagata
    16561 ttcaaaatgc tgggaactca ggagaatttt atacgccacg tgcagcgact gattttattg
    16621 ccgaagttct tgacccaaaa cttggagaat caatggcaga ccttgcttgc ggaacaggag
    16681 gcttcttgac ttcgactctg aaccgtttaa gtagtcaacg taaaactagt gaagatacca
    16741 aaaaatataa tacagctgtt tttggtattg aaaagaaagc atttcctcat cttttagcag
    16801 ttacaaatct gtttcttcac gaaattgatg accctaaaat tgttcatgga aatactttgg
    16861 agaaaaatgt tcgtgaatat acggatgatg aaaaatttga cattattatg atgaatccac
    16921 cttttggagg gtcagaatta gaaacaataa aaaataactt tccagcagaa ttacggagtt
    16981 ctgaaacagc tgatttattt atggctgtca ttatgtatcg tttgaaagaa aatggtcgtg
    17041 ttggagttat tttacctgat ggttttctat ttggtgaagg tgtaaaaact cgcttgaaac
    17101 aaaaactggt agatgagttc aacttgcata cgattattag gttgcctcat agtgtctttg
    17161 caccgtatac aggaatccat acgaacattc ttttctttga taaaacaaag aaaacagaag
    17221 aaacttggtt ttatcgttta gatatgccag atggttataa aaatttctcg aaaactaagc
    17281 cgatgaagtc agaacacttc aatcctgttc gtgactggtg ggaaaatcgt gaagagattc
    17341 tggaaggtaa gttctacaaa tctaaatcat ttacacctag tgaattggct gagttgaatt
    17401 ataatttaga ccagtgtgac tttccaaaag aggaagagga aatcttaaat ccctttgagt
    17461 tgattcagaa ttatcaagcg gaaagagcaa ctttaaatca taagattgat aatgtattag
    17521 ctgatatttt gcagttgttg gaggacaaat aatgacacca gaacaactta aagcaagtat
    17581 tctccaaaga gcgatggaag ggaaattagt gccgcaaaat cccaatgacg aacctgcaag
    17641 tgaattatta aagagaatta aagctgaaaa agaaaaactt atcagtgaag gaaaaatcaa
    17701 acgagataaa aaggaaactg agatatttcg tggtgatgat g
//
LOCUS       DAVKNL010000042        17679 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVKNL010000042 DAVKNL010000000
VERSION     DAVKNL010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17679
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.042"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            1..692
                     /locus_tag="VW411_001444"
                     /pseudo
     CDS             1..692
                     /locus_tag="VW411_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(809..1156)
                     /locus_tag="VW411_001445"
     CDS             complement(809..1156)
                     /locus_tag="VW411_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9794162.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYANDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(1267..3042)
                     /gene="spxB"
                     /locus_tag="VW411_001446"
     CDS             complement(1267..3042)
                     /gene="spxB"
                     /locus_tag="VW411_001446"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEU9794163.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            complement(3249..5492)
                     /locus_tag="VW411_001447"
     CDS             complement(3249..5492)
                     /locus_tag="VW411_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU9794164.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDVIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQSLHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            complement(5502..5873)
                     /locus_tag="VW411_001448"
     CDS             complement(5502..5873)
                     /locus_tag="VW411_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU9794165.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QAGEFGFACGMNMMHGKMIVE"
     gene            complement(5884..6279)
                     /locus_tag="VW411_001449"
     CDS             complement(5884..6279)
                     /locus_tag="VW411_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEU9794166.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVDKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     regulatory      6463..6561
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6645..7436
                     /gene="thiD"
                     /locus_tag="VW411_001450"
     CDS             6645..7436
                     /gene="thiD"
                     /locus_tag="VW411_001450"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU9794167.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSNARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     gene            complement(7646..8278)
                     /locus_tag="VW411_001451"
     CDS             complement(7646..8278)
                     /locus_tag="VW411_001451"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU9794168.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARKVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPV
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLKKLHNILS"
     gene            complement(8271..9074)
                     /locus_tag="VW411_001452"
     CDS             complement(8271..9074)
                     /locus_tag="VW411_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU9794169.1"
                     /translation="MQEFTNPFPIGSSSLIHCITNEISCEMLANGILALGCKPVMADD
                     SREVLDFTKQSQALFINLGHLSAEKEKAIRMAASYANQSSLPMVVDAVGVTTSSIRKS
                     LVKDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLVVSKNQVAVLGNGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            complement(9074..9598)
                     /gene="thiW"
                     /locus_tag="VW411_001453"
     CDS             complement(9074..9598)
                     /gene="thiW"
                     /locus_tag="VW411_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEU9794170.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFHYSALGE
                     ILGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            complement(9603..10295)
                     /gene="tenA"
                     /locus_tag="VW411_001454"
     CDS             complement(9603..10295)
                     /gene="tenA"
                     /locus_tag="VW411_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEU9794171.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEISDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            complement(10306..10956)
                     /locus_tag="VW411_001455"
     CDS             complement(10306..10956)
                     /locus_tag="VW411_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEU9794172.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALTLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSVSLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            complement(10958..12343)
                     /locus_tag="VW411_001456"
     CDS             complement(10958..12343)
                     /locus_tag="VW411_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9794173.1"
                     /translation="MGLELRAIQSPIFSESFDFTFHAQAFTLLVGSSGSGKSSLFQMI
                     AQVSSLPYSGQVLIDGSEVSQLSIVERVQTVGILLQNPNHQFTMESLFEELVFTMENI
                     GYHLQEIDSKIAEVVQQCRCKDILHRLIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELVYDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGSITWKGSVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            complement(12344..12904)
                     /locus_tag="VW411_001457"
     CDS             complement(12344..12904)
                     /locus_tag="VW411_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9794174.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGFVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     regulatory      complement(13166..13254)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(13463..14092)
                     /locus_tag="VW411_001458"
     CDS             complement(13463..14092)
                     /locus_tag="VW411_001458"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU9794175.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFGTQSKDDAGGTIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
     gene            complement(14094..14876)
                     /gene="thiM"
                     /locus_tag="VW411_001459"
     CDS             complement(14094..14876)
                     /gene="thiM"
                     /locus_tag="VW411_001459"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU9794176.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVMIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLKTAVLVY
                     NIAGEIAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            complement(15113..15781)
                     /locus_tag="VW411_001460"
     CDS             complement(15113..15781)
                     /locus_tag="VW411_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEU9794177.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     regulatory      complement(15996..16084)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(16338..17474)
                     /gene="lctO"
                     /locus_tag="VW411_001461"
     CDS             complement(16338..17474)
                     /gene="lctO"
                     /locus_tag="VW411_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEU9794178.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCNVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGTQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
BASE COUNT         5323 a         3895 c         3249 g         5212 t
ORIGIN      
        1 ttaggtcgtt cctacacacg tgatgccttc tatcaactgt tgaagcgcca tggttggcga
       61 aatattacgc cacgtccaga acatcctaag aaagcagacg ctcaaaccat tgttgcgtct
      121 aaaaataaaa tctcaatcca agaaggcaag aaagcgtttt aaatatagta gacgttttcg
      181 taaggtttgc ttgatgtacc aagctgaagc tggtttcggt agaatcagta aactgggatc
      241 ttgttgggct ccaataggag taggtccaca tatccatagt cactatatac gagaatttcg
      301 ctattgttat ggagctgttg atgcctatac aggcgaatca tttttcttaa tagctggtag
      361 atgtaatact gagtggatga acgccttttt agaagagctt tcacaagctt atccttttac
      421 tcgttatgga caatgctata tggcataaat caagtacctt aaagattccg actaatattg
      481 gttttgcatt tattcctcca tacacaccag agatgaaccc cattgaacaa gtgtggaaag
      541 agattcgtaa acgtggattt aagaataaag cctttcgaat tttggaagat gtcatgaatc
      601 aactccaaga tgtcatacaa ggattggaga aggaggtgat aaagtccatc gttaatcgga
      661 gatggactag aatgcttttt gaaagcagat gagtattata tgcaatttct ttatataaaa
      721 agaccggatt gctccgatct ttcaatagtt catattctca atttctattt taaaaatagc
      781 taaggttaac gtcaaatgac tacgcgacct atttcatacg ataaaaatca agcactagac
      841 cagcaggtcc ttgaactaat aaggactctg ttccccaatc ggttacagtt ggtccgtgta
      901 aaacctttat accaagctcg ttcaaccgtt tgtagttctg gtctacatcc tcaacctcga
      961 tatgaataat gattcctgac tgaaagtttt ccaaaggaac caaatgattt tgtgacaaca
     1021 taaggcagtg actaccaatc gtaaactgag caaaaccatc attagcataa tctgcctttt
     1081 tatccaagat atgctccaag tcagcacaga cttggggaac atttgaaacg ataatatcta
     1141 attgatttaa attcatttac tctcctccat aaaaagaccg gattgctccg atcttttaaa
     1201 gttctgctct atgaaaatca aagaataaag tctacaagtt tcatatttga ttttcggcga
     1261 gaggaattat ttaattgcgc gtgattgcaa tccttcttct tccaagaaga gacggaatgg
     1321 tacgagttct tctgcttcgt atttttcctt gaaggctttg atagcttctt ctgagtgaag
     1381 ttttggatcc aattcaagta cttctactgg aagtggacgg tgttgagtga tgcgagcatc
     1441 gatgacaaca gttttacctt ctttgttcaa tttaacagct tctgcaacaa ctgcatcgat
     1501 gtcttcgata cggtcaactg tgaatccaac agctccttga gcttccgcaa ttttagcgta
     1561 gtcagcgttt gtgaagtcta caccaaacaa gtgtttgttt gtatcttcgt atttgttctt
     1621 gatgaagccg tactcagcat ttgagaagac aaggttgata actggaaggt cgtattgaac
     1681 gtttgtgata acgtctgggt agcacatgtt gaatgctccg tcacccatga tgttccatac
     1741 ttggcgatct ggattgtctt tcttagcagc gataccacca ggaagggcaa tacccattgt
     1801 cgcaaagagt ggagatgtac gccacatgtt cttaggtgtc atgtgaaggt gacgagtaga
     1861 tgtttgagta gtgttaccta cgtcgattga gtagatagcg tcttgatcag catgtttgtt
     1921 gattgcattg taaacttgat acaattgcaa ttcaccctca gttttacctt cgagtttgtt
     1981 catgtaatca cgccagtttt ggttgttctt aacgtttgca cgccaccatg gagttgattc
     2041 aactgggttt actttgtcaa ggatagcttt agctgcttga ccagcatcac caaggattga
     2101 agcgtcaagg gcatgacgtt taccaagttt gtaagggtcg atatcgactt ggatgaattt
     2161 ttcagtgttc ttgaatgctt cgtaaacttc agcaaatggg aagtttgaac caaggaaaag
     2221 aactgtgtct gcttcaaaga ccacttcgtt ggctggtttc caaccaacac ggtaagcaga
     2281 acctgtcaaa ccttcatagt tccattcgaa agcttcaaag tttttaccag ttgtgatgat
     2341 tggtgctttg attttacgtg acaattcagt aatcacttca ccagctttaa caccaccaaa
     2401 tccagcatag ataactgggc gttcagcatt gttcaagatt tcaacagctt tgtcgatttc
     2461 aacttcgttc aaagcaggag cgatgaatga gcgttcgtat gaacctgaac cgtagtatga
     2521 gttttcatcg atttcttgga aaccgaagtt tactggaatt tcaacaacag ctggaccttt
     2581 tttagaaact gcagcacggc aggcttcgtc aattactttt ggcaattgct cagcgtaagc
     2641 tacacgtttg ttgtaaacag cgataccgtt gtacattggg ttttggttaa gctcttggaa
     2701 agcatccatg ttcaattcgt taactggacg tgatccaagg atcgctagga atggagtgtt
     2761 atccatagct gcatcgtaaa caccgttaat caagtgagtc gcacctggac cacctgaacc
     2821 aactgcaacc ccgattgagc cgccgaattt agcttgcata accgctgcaa gagcacctgt
     2881 ctcttcgtgg cgaacttgta agaaacggat atctttgtct tcagccaaag cgtccatcaa
     2941 tgagctgagt gttcctgatg ggataccgta gattgtatct acgccccatg ttttcaatac
     3001 gttaagcatt gctgcagatg cagtaatttt cccttgagtc ataatgataa ctctccttca
     3061 atttttttaa acttggagaa tacgattaca tagaattgga aacgttctcc aaatttttac
     3121 tattccactg tatcatattt atgctgactt ttctaaaaat ctgctcaaaa ctctctattc
     3181 tctattctaa tacagttttg aaagttctgt catttctgtt ttataacaaa gaaatctagt
     3241 cattactttt agtctatttt actaaaattt aacagaaggg aactggtcag aacagataca
     3301 gaactaaagg ccatggctag acctgccaat tctgggttga gagccagtcc aacacctgaa
     3361 aagactcctg ctgcaatcgg aattccgaca acattgtaga taaaagccca gaaaagattg
     3421 agtagaattc gatgaaaggt tttcttactc atatcaaagg cacgaaccac tcctaaaaga
     3481 ttattggttg tcaacaccaa atctgctgac tcgatggcga tatctgttcc agctcccata
     3541 gcaatcccca catctgctac actaagggca ggagcgtcat tgataccgtc cccaacaaag
     3601 gctactttcc ctgactgttg cagtttatgg atttcatggg ctttttcttc tggcaagacg
     3661 cctgcaatga cctcttcaat tccgatttga tctgcaatag cacgcgccac accagcattg
     3721 tctcctgtca gcatgactgt tcggagacca cgttttttta gctgactgat ggctagctta
     3781 gcattttcct taggaatatc ttgcaaagca agcaagcctt tgatttcatt gtcaacagct
     3841 aagaacacaa ctgtcttagc ttctttttct agttcttcta gtttatcttg ataagtatta
     3901 gaaatatcca tgccatccag cattttagca tttccaagta aaacttgttt tccattgatt
     3961 cgccctgaaa cacctttccc gtgcaaggac tgaaaatttt caacagtttg aaactcaagt
     4021 ccagcttcac tcgctcgctt aacgatagcc tcagccagtg ggtgttgaga agcatcttcc
     4081 aaggaggctg ccaacccaaa cacttctact tcgtcgccga tgacatctgt taccacaggt
     4141 ttcccttccg tcaaagtccc ggtcttatca aagacaaggg tttgaacttt ctggatttcc
     4201 tgtaagacag ttccattttt gaggagaacc cccatcttgg cactacgtcc tgtccccacc
     4261 ataagggctg tcggtgttgc aagtcccaag gcacaaggac aggcgataat caaaaccgcc
     4321 actccgtaga gaagagagga cacaaagcta gctccaagca caaccacact atccctgagc
     4381 aagacgaacc aaacccaaaa ggtcatgatt cctaaaatga caactactgg gacaaaaatc
     4441 cctgaaatct tatccgtcaa gtcctgaatc ggcgcacgac ttgtctgagc tttcttcaca
     4501 aaatccacaa tctgagccaa aacagtctct gagccaactt tttctgctct aaagacaagc
     4561 gttccactat gattgatggt tgagccaatg acagtatctc caactgtctt gtccacaggc
     4621 agactctcac ctgtcaccat ggattcgtca atactagaga caccttctac tacgacacca
     4681 tcaacagcaa tcttttcacc gggacgcact cgaatcaggt cgcctacctt gacttgttcc
     4741 aaaggaactt ggacataact atcatcactc aagacttctg cggttttagc ttgcaagtcc
     4801 agtaatttct ccacagcttg ggacgtattt tttctcattt tttcctcaaa aaccgctccc
     4861 aaaagaacga aaaagaggat aaatccagca ctttcgaagt aaacagggag accagcaaag
     4921 agagcaacta ggctatagaa ataagccact agagttccca gcgcaaccaa ggtatccatg
     4981 ttggcattgt gctttttaaa actggcccaa gcactctgga tatatggctt acctgcaact
     5041 aacataatag gcgttgttgc tagaaaggtt ccccaatgca tgacttgatg actaatgcta
     5101 cctgtcaaca tcccaatcat gagaatcaca agaggcacag taaagatact agtaatccaa
     5161 aaacgttgca ggagagatag agattttcga gtcttctcaa cgactgtata gcttcccttt
     5221 tgcatcttca tgccacaaga aaattcatgt cgccctaatt cttgaggcgt aaaacgaatg
     5281 actttctcct catctacgcc gattggttcc aagatacctt cttcttcaaa cagaatttcc
     5341 ttataacagt ttgaaggagt agcacgatga aaggtaatct cagctggaat tcccttttga
     5401 agctggatat gggctggatg atagcctttt tcagctcgga tacggatttt ttgaatgcca
     5461 ttttctaagc ttgctttcac aatttctgtc atagtctcca cctactctac aatcatcttg
     5521 ccgtgcatca tgttcatacc acaagcaaag ccaaactctc cagcctgttc aggcgtgatt
     5581 tccactacat actcttcccc cattggcagg ttcgcatgta caccaaaatc tggaaaaaca
     5641 atttgatcca gacatggtga aggatccttg cggtcaaaga caatgcgtgc tggcactgat
     5701 ttcttgagga caatcaactc aggagtatag cctcccatga cttccactcg aatctcttgg
     5761 tatccgtttt tttgctgggc tttttgtcca gatttttcag gctttttgaa aaaccaaaac
     5821 aagataaacg cgataagggc aatacaaata atggttacaa tactatttaa catgacgtct
     5881 cctttacata caattacatc ttacttctgt tacagcactt gatttcttct ctgaaatcac
     5941 agcttccaag tcttccaagt cagtctgagt aaattcacat tctacaatca agtcagccaa
     6001 caaattccta atcctacggg aacaaacctt gtctttgata tcttggacaa gtaaatcccg
     6061 actttggtct agagttaaaa gggctgaata aacaaaggac ttgccttctt ttttccgagt
     6121 caaacactct ttatcaacca gacgagccaa aagtgtctga accgtggact tggaccagtc
     6181 aaaccgctct gccaaaaccc taatcaaatc tgtactggtc tgctccccct gcatccaaat
     6241 aatcttcatg acctgccatt ctgcatctga aatctgcatt accatacctc caaaatctac
     6301 atttgtcaat tacactcatc agtatactct taaaatctac atttgtcaat tatagaaata
     6361 atattttctt cgaaaaatag aattttaatc atttgaaaaa cgatttgcag tcaaatatta
     6421 ctatataaac aataaaaata tgctatacta aagaaaaaag aaaacaacca ctaggggtgc
     6481 gtaaagctga gattaacgac tgttagatcc ctctgactca atctaggtaa tgctagctga
     6541 tggaagtgga aatgataatg ggaactagca gtcttctatt gcctttctaa aacagactag
     6601 cttgttctta agaatacaaa cttcagttgg ttgggaggtt ttagatgact tatttacccg
     6661 ttgctttgac cattgcaggg actgacccta gtggtggtgc tggcattatg gcagatttaa
     6721 agtcattcca agcgagagat gtctatggaa tggctgttgt aaccagtctt gtcgctcaaa
     6781 ataccagagg tgttcagcta atcgagcacg tttctcctca aatgttgaaa gcccaattgg
     6841 agagtgtctt ttctgatatt ccacctcagg ctgtaaaaac tggaatgttg gctactactg
     6901 aaatcatgga aatcatccaa ccctatctta aaaaactgga ttgtccctat gtccttgatc
     6961 ctgttatggt tgctacaagt ggagatgcct tgattgactc aaatgctaga gactatctca
     7021 aaacaaactt actacctcta gcaactatta ttacgccaaa tcttcctgaa gcagaagaga
     7081 ttgttggttt ttcaatccat gaccccgaag acatgcagcg tgctggtcgc ctgattttaa
     7141 aagaatttgg tcctcagtct gtggttatca aaggcggaca tctcaaaggt ggtgctaaag
     7201 atttcctctt taccaagaat gaacaatttg tctgggaaag cccacgaatt caaacctgtc
     7261 acacccatgg tactggatgt acctttgctg cagtgattac tgctgaacta gccaagggca
     7321 agagtcttta ccaggcagtt gataaggcca aggcctttat cacaaaagct attcaagatg
     7381 cccctcaact cggtcatggt tctggtccag tcaaccatac aacttttaaa gattaagaaa
     7441 aaaaactctc tagttcccac tttaagggaa ttagagagtt tttatactct tcgaaaatct
     7501 cttcaaacta cgtcagcttc catctgcagc ctcaaaacac tgttttgagc tgacttcgtc
     7561 agtcttatct aaaacctcaa ggcagtactt tgagcaacct gcgactagct ttctagttta
     7621 ctctttgatt ttcattgagt attaattagg aaagaatgtt atgcaacttt tttaaaaagg
     7681 cttgcgtttt tgcctcaata tcttctgctt gcatcaaatc acgtacaaca gctacaccag
     7741 ctatgccagt gcccataagc tgatcaatat tctccgaagt caagcctcca atagcaacta
     7801 ctggaatggc aaccgtttgg caaattgttt tcaaggtcga tatcagagta atgggcgcat
     7861 tttccttggt ggtggttggg aaaatggctc ctgtacccaa gtaatctgca cctgatttct
     7921 ccgcttccag agctctttta accgttttag cggtgacacc gaggattttt tcaggaccca
     7981 agactttgcg agctaccgaa actggtaatt catcatctcc gatatgcaga cctgctgcat
     8041 caaccgcaag acaaacatcc aaccgatcat cgattatcaa gggtacctga taagcatctg
     8101 ttatttcctt gacttgtttt gccagttgat aatattgatt ggttgtgaga tttttttctc
     8161 gcaattggac tatggtaacc cctgaacggc aggccgtctc aacttttgca agaaagcttt
     8221 ccacggaatc ttgatagcga ttggttacca gatatagtct aagtgcttct ctattcataa
     8281 acctctcctt tgatggtatc tagccaattt tcatctcttc ttaggagcga aagctgattg
     8341 agtacttggt aacgaaattc ttccaatccc attccttgaa caactatttt ctcagcagcg
     8401 atattgagat aagagactgc taagcaagaa gcttcaaaac cagtctttcc ttggctgaga
     8461 aaaacagctg ttaaggctcc aaccaagtct cctgtccctg ttatccagtc taattcagta
     8521 cagccatttc ccagtacagc gacctgattt ttcgaaacga cgaggtcctt gggacctgtg
     8581 actaagaaag acataccagg ataggtctga caccagtctt tcaagacttg aagcaaatcc
     8641 tccgtttctt gatctttagc actcgcatcg accccaacgc cgtggtgctt taatccaaca
     8701 agacttcgaa tttctgacat gtttccttta aggaccgtag gtctatagtc taaaaggtct
     8761 ttaactaagc tcttacgaat ggatgaagtc gttacgccaa ccgcatctac taccatcggg
     8821 agagaagatt ggtttgcata cgaagctgcc atgcggattg ctttttcctt ctcagctgac
     8881 aaatgcccca aattgatgaa gagagcctga ctttgcttag taaaatcaag aacttcacgg
     8941 gaatcatctg ccatgacagg tttgcatccc agagccaaaa tcccatttgc cagcatctca
     9001 caagaaatct cattggtaat gcagtgaatg agggaactag agcctatagg aaagggattt
     9061 gtaaattcct gcatcagtct atcctttcac taaagaaata tccctgcact tttttaaaga
     9121 attcctgctt gattaaaaat cgaaaggcaa taaaggaaat cgctgtacca atcaaggttg
     9181 ctccgaaaaa tcgaggcgtg tagataaacc agctaagctt agcagctgat cctgtaaaga
     9241 gtaccataac aggataggaa acaatggaac caataatacc tgttcccaaa atctctccta
     9301 gagcagaata gtgaaatttt cgaccgtact tataaaagag acctgctaga agggctccaa
     9361 aagtcgctcc tgtgagagct aaaggcggaa tcccttgagt cgtcatacgg ataaaggctg
     9421 tgactgtagc catagccaag gcataaacag gtcccatcat gattcctgct agaatattga
     9481 ctacactgga catcggtgcc attccctcaa ttcgaaagat aggtgtaagg actacatcaa
     9541 gggcaatcat catagataaa atggttaatt tgtgaacttg taattggtgc tttctcatgc
     9601 ttctattctt ctcctttttc taaagactgt aaatcgctct tccatgtctg gtgttggtag
     9661 gccatttccc aaaacttggc ttccatatga acactgatgt ggaaggcatc tagcattttt
     9721 tgcttgtctg tctcgtcact ttctcgatag agctgattga ccagtgctcc ctcctctctg
     9781 atctgttgct ctaactcatc cgtaatataa gtttcaatcc attgttgata gagaggattt
     9841 ggtgatggtt taagattaag tgatttgcct atatcatggt ataaccaagg acaaggaagc
     9901 aagcttgcaa aagcgatggc taagttcggt tctgcaaatt gcctataaat atgagaaatg
     9961 taatgataac aggttggagc gattggatgt tgctccattt cctggtcgct gatttccaat
    10021 tccttgaaaa attgttggcg aataaataac tcaccctcca ctaaaccctg agcattttgt
    10081 ttcaagagtc ttttcatctc ttggtttgaa gtcttatcag ccaaaagatg atagatttct
    10141 gagaaagcct tcagatagta ggcatcctga atcaggtaat agcggaaaat ggcaggttct
    10201 aaattcccct cttgtaattg taaaataaag ggatgatgaa aggaagcctg ccaagctttc
    10261 ttggataatt ccatcgcaat atctgtaaat tccataataa ctcctttata aaaatagact
    10321 ggtttgaagc aataaaaaga aaagcaggta gattaatttt gtttttttag gaatataaaa
    10381 agtccgatag ctattcttca actgtgcatg ttcgtcatat ccgtgagcag atagagctct
    10441 caggtaaaga tggcgccacc taaagactgt catcagaacc ttactgtaaa tcaagggcga
    10501 ccaaaaatgt agttcttgac cacgtaatag gcaagcttct ttgagggact tgatttcttg
    10561 ctgaatgaga ggaaaagaat tgaataccac aatcaaggca taggaccaag agcgtgatag
    10621 ccccttttga gccaagtaca agagaagctc ttttagtgaa acagaggaaa caaagacaag
    10681 gccgatacaa actgtcacaa aggccctcgt tccaagcatg actgcctgtg aagcatctcc
    10741 gtgtaactga actgcccagt agttggcaaa agatggtaaa atggcaagta tgatcatcca
    10801 agctaacatt ttaaatcgac ggtaatagag cataaagaga atacaaaatg cgactaccga
    10861 aagagtcaga gcaatcgaag gaatgaaaga tgtttccaag gataaaatca gcaagaagag
    10921 actgataatc ggtgtctggg ttgctacttt gaccatacta tctcacctcc ccttgggtat
    10981 tgctactctg agatgtaagt ggtttggtaa tggtcacttc tttcacatgc cgaagaccct
    11041 gactagtcat ctcaatccaa taatcaacca cagaaatcaa agggtctaaa cgatgactaa
    11101 tgagcagaaa acttcttcct tgattcctct cctccacaat ccacttgcaa aaataatggc
    11161 aggctctatc atccaaacct gcaaaaggtt catctagcaa gatcacggaa gccttactgg
    11221 tcaagatggt caggagctga agaatttttt gctgaccacc acttaattga tagggactct
    11281 tatcgactgc ctgctccaaa tcaaaatatc gtaaagcttg aaaaatccgc tgatttcttt
    11341 cagaatcagg tccatctaat tgaagctcct ctcgcagact gactcggata aactgcttct
    11401 cagcttcctg aacaacacca gtcagatcac gatacaaact ctttttcttt ttcaggaccg
    11461 aacccttcca agtaatgctc cccttatact tttgaaattg aagaatagac cgaaagaggg
    11521 ttgatttccc gacaccattg tcacccagga tacaggaaat cccttgatag aatgtgaaat
    11581 cagcaattga aaagaggggg cgattaccaa gctcaccagt cacacggttc atatggaata
    11641 gttccgggct agaagcaact tcctttgaag caacctgtgt catctcatag gaagggattt
    11701 gaaacacttc ccttagtttt ccgtctctta gctccaccat atggtcgata taggctttat
    11761 agtcagataa atcatggtcg cacaaaataa ctgtcttccc atcatagacc aactctttta
    11821 gaatctccaa tatctcgatt ctgctcttgc ggtcaatgga agcgaagggc tcatccaaga
    11881 gatagaccct aggattcatg gcaaagagga cagccagcgc tgctttttgc ttttccccac
    11941 ctgataagtg atggatgaga cggtgcaaga tgtccttgca acgacattgc tggacaacct
    12001 ctgctatttt agaatcaatt tcctgaaggt gatagccgat attttccatg gtaaaaacca
    12061 actcctcaaa caagctctcc atggtaaatt gatgattagg attttgcaag agaataccaa
    12121 ccgtctggac acgttcgacg atagaaagct gactgacctc gctcccatct atcaggactt
    12181 gaccgctata gggaagagaa ctaacttggg caatcatttg aaagaggctg gattttccag
    12241 acccactact cccaactaac aaggtaaagg cttgcgcatg aaaagtaaaa tcaaacgact
    12301 cagagaagat tggggactga atcgctcgta gttccagacc catctatgct tttcctccag
    12361 ttgcaaactg atgatagagt ttgacaatgg cacgaaccaa gatggtacag aagaaataaa
    12421 cagaaataaa acgtaccaca agcaaggaaa ggacaaacgg aagggaaaag gcgtagtaac
    12481 ctaacttaat gtattcatag acaaagctaa caagcgtaat cccaatacta ttagcagtta
    12541 gagagagcca actttcatag cgattcttag ttacgataaa accaaattca cttcccaaac
    12601 cttgaacaaa gccagacaaa agagctccta gaccaaattg gctaccataa aggacttcag
    12661 caagcgcagc tagcacttct ccaatcgttg cacttccgac tctcggaaca aagatggcag
    12721 caatgggcgc agccatacac cagagaccga agaggatttc attggcaaag gcctgcaaac
    12781 caagaggtgt taagagtaga ctgagaatat tatacacata tcctgaacca acgaaaaccc
    12841 caccaaaaaa gatagacaag aaagcaagca agataacatc ttttaactgc cattttttca
    12901 acataaaaaa ctcctttttt taaagaaaag tgaggcactc aagaagaccg acctaaatac
    12961 tttgtatagc agactgaatt tagaacagta cacaagaaca ctaaaatatt tctagaaatt
    13021 aatttgaatt ttctaattga tttgttcgca tcttatttca atctactata tcatcttcat
    13081 ccagtttcgt aaaagaaaaa actctaatta cagatacaaa ttagagttca gcttacaaga
    13141 ttagacagtt cttttcgaca tacgaaaaaa acatttcaca tttcccttcg ccagtcttaa
    13201 ctgtatcagg ttcaatgggt atcatctcag cctaaagcac cccaaatgtc tttattattt
    13261 aattatgtga ttattataac acacatttta tactagttca agaaattgaa ctggaaatac
    13321 agccttgcac tcacaaagac agcagatctt tcttttgcaa aaaacaaatg acctgtttga
    13381 tgaattagcc attcaagctg aatctggaca tagcttttta aaaaaggaaa atcctactta
    13441 cttagaatcc aaggatagat atctattgtt cactcatttc ccgaacagtt ttttctatat
    13501 tttttgcata cgatattgcc gaaatgattg aaacgccatc catattggtc tttataatgt
    13561 ctttaatatg tttcgtctgt atcccaccaa ttgcaactaa aggcatttgt ggcaatagtt
    13621 ttctcatcaa ttcaagacct tcataaccta tagtaccacc agcatcatcc tttgactggg
    13681 taccaaatac aggcccaaca cctacataat ctacatattc aacttttgat tgttgaaatt
    13741 cttcttcgtt tcttatagaa agaccaatta ttttatctgg catcaatttt ctaatttcat
    13801 caacaccaat atcatcttga cctacatgta cgccatcggc gtcaatttcc attgctaaat
    13861 ctatatcgtc attaacgata aatggaacat tgtatttttt acaaagttct ttaatttgga
    13921 tagctagctc aagtttttct aagccttcta aagcaccctc acctttttct cgaaattgaa
    13981 ataaggttat accacctttt aaggcttcct caacgactgt atatagattt tttccttggc
    14041 aagtagtcgt tccacaaata aaatatagtt ttagtaattc tttatgaaac atcttacttc
    14101 actcttttga attcctttac atcttcatct gtaatctcgt ataaggcatt tataaattca
    14161 actttaaatg tcccaggaag atgtccattt ggacgttttt ctgctatttc tccagcgata
    14221 ttgtaaacca acactgctgt ttttaatgat ttcaattctt gacctttttc tagtccgata
    14281 aagcttgcta ctacagctcc taataagcat cctgtcccaa tgactttcgg catcatagca
    14341 ctaccattat gaatcattac cacttctcca ttaacagcaa tggcatccac ttcacctgtt
    14401 actactattg gaatattgaa cttctcattt gctgctagag caatttcgtc aatattatct
    14461 acgcccgcac tatctactcc tttagatgcc acatctattc ctactaaaga ggcaatctcg
    14521 ccagcatttc ctctaatcgc tgctagttta taattgttga ttagatcatc tgctactttt
    14581 tttctatatt ctcctgctcc acaggctaca ggatctaaaa ctgctgggac attatatttc
    14641 tctgcaattt tcagagcagc ttggtataat ttccaatttt catctgtcaa tgttcctatg
    14701 tttattaata aaccaccagc atactttaac aaatcctcta aatctgctgg aaactcactc
    14761 atggctggtg aggcgcccag tgctactaat ccatttgctg tgaaattttt tactacatca
    14821 ttggttatac aaatgaccaa tggtgctttt tcttttaata attttaaact tgtcatattg
    14881 aaatccttcc ttttcacttt atacgatcta ctaatttcga tttatcttta gttgagaatt
    14941 tttttcattt acattgaatg atttatactc aatgaaaatc aaagagcaaa ctaggaggct
    15001 aaccgcaggt tgctcaaaac actgttttga ggttgtggat agaactgacg tggtttgaag
    15061 agattttcga agagtcttac ctcatcaaat ttgtaaatat catgagcctt ctctagacat
    15121 cgtaaccaat atcaaaaaaa gctaattcta aagcgactgc ttgattccag cgttgctgaa
    15181 gttctgtcaa atcttctcga tttttaccga cacgattgag ttcgtcaacc agaaattgaa
    15241 cccactctgc aaagaaagga cctctgtgga gattgatcca ttccgaatga atatagactt
    15301 caggtaaagc caaatcttta gaaccccagt ctaaatagag accttctgca atgaccagca
    15361 tgaccaaaag atgggcatag tctgatgaag ccaccgccga atacattaga tcctgaaagg
    15421 cttttgttac agggtgcaaa gtcacttcta gatagtcatt ctctgctact tttaactctt
    15481 taaaagcctt ttggaaataa ccatcttcat ctgcttcaag aaagcctagt tgcttggcaa
    15541 aacgaagctt ggattcaagt ttatctgcgt gagctacgca ggcacccagc atggataaga
    15601 aggcatcaaa gaagtgataa tcttgaatca gatagtcctt taagacctta ttctcaattg
    15661 tccccgcaaa aagttcctta acaaaacgat gattgattgc agcctgccaa tccttctgac
    15721 tgctttttaa taattctcca acagtcaaac ctggctgaaa tgcatagtct tgtgtttcca
    15781 tatttacttc tcctctcttt acttgttagt aattaataaa acaccaagaa atatcaagca
    15841 aaatcgtaat tccacttgat ccttttaaag cacatcgaga gcatttgcag agagctaact
    15901 aaacaagcct atccagttta tataaacaaa aaactccaat tacaatcaag aattagagtt
    15961 gacttacaag attagaccgt tcatttcacc atacgaaaaa actgttcaca tttcccttcg
    16021 ccagtcttaa ctgtatcagg ttcaatgggt attatctcag cctaaagcac cccaaatgtc
    16081 tctattattt aactactgaa ccagtatagc aaaaaatgaa agccctagca agatatttga
    16141 ccgaaaaata tctttatata taatatattg aaactagaat agtacacctc tacttataaa
    16201 acattgttag aaatcgattt gactgtcctg attgatttgt cctattctta tttcatttta
    16261 ctatagtttt cgatagcaat ttattcttcc aatacacgaa gaaaaacctc cacattcagt
    16321 ggaggcaatc tgttttatca atacaatttt aagtcacgag ggtcaactgg gaaggttggg
    16381 ttgtatggat tgtgacggag cttgaagtgt ttgacatctt caatggtctg agttccagac
    16441 aattgcataa ctgtcttcaa ttccgcattc aagtgttcaa agacttgacg cacaccgaca
    16501 ctaccaccga gagccaagcc atagatgaca gggcgtccaa tagcaaccaa gtctgctcct
    16561 gatgccaagg ctttaaagac gtgttgacca cgacgaacac cagagtcaaa gacaatcggc
    16621 acacgtctat caactgcttc tgccacttct tgaagcgagt caaaggcagc tggtccaccg
    16681 tcgatttgac gaccaccgtg gttggttacc cagataccag aagctcctgc agcaagcgaa
    16741 cgttcaacgt cctcacggca ttgtggtccc ttgacataca caggaagacc agagtattca
    16801 gcgataaatt ctacatcgcg tggagacaag cgttgtttag ctgatttgta aacaaagtcc
    16861 attgatttac cagcaccttc tggcaggtat tcttcaacaa tcggcatgcc aactgggaag
    16921 acaaaaccat tacgcttatc cacttcacga ttccccccta cagtagcatc tgccgtcaag
    16981 acaatcgctt tataaccttc agccttcaca cggtccatga tgtggcggtt gataccgtca
    17041 tccttactaa agtaaaattg aaaccaatga ggtgtccctt ggagggcttc agaaatctct
    17101 ggaaggtcaa cagtagagta agaactggtt gtataaagag aaccaaactc atgcacacca
    17161 cgcgcagtcg ccacttcccc ctgttcattt gccaatttat gagccgcaac aggtgccata
    17221 atgattggag aagatagttt ttcacctgca aattcaatct ctgtacttgg attttctaca
    17281 ttgcaaagtg tatgaggaac gatgagcttg tggttaaagg cacggatatt ctctcttaaa
    17341 gtgaaagtat cttccgcccc actagcgata tagccaaatg ctgctttagg aataacttgt
    17401 tgcgccattg gctccaaatc ataggtattg atgaagtcta catgaccttc tgcattgctt
    17461 gttttgtatg acataaactg tcctcctcga ttaagtaagc gtttgctttt tactatacaa
    17521 ctataataac tcttttttca atcattttca agagttttgc ttaaaaacca tcttattttt
    17581 tcatacaata tactagccct actttcctta aactctaaac cttagtctat attaagtgaa
    17641 tcgttgagat tttttgctgc caggcatttg aactacttt
//
LOCUS       DAVKNL010000043        17023 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVKNL010000043 DAVKNL010000000
VERSION     DAVKNL010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17023
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.043"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(<1..361)
                     /locus_tag="VW411_001462"
     CDS             complement(<1..361)
                     /locus_tag="VW411_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794179.1"
                     /translation="MKKTLTPTKESIDSLRNYEASKQLTDTRFKRLVGVQRTTFKEML
                     AVLKTAYQLKHAKGGRKPKLSLEYRTYEEIAADFGVHESNLLRRSQWVEVTLVQSGVT
                     ISRTPLSSEDTVMIDATE"
     gene            complement(306..488)
                     /locus_tag="VW411_001463"
     CDS             complement(306..488)
                     /locus_tag="VW411_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9794180.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KNIDTYQRVNRLPAKL"
     gene            823..2568
                     /locus_tag="VW411_001464"
     CDS             823..2568
                     /locus_tag="VW411_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HEU9794181.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEI
                     HIKNKSTEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETD
                     VAKPKKNSNQGVVGWVKDKGLWYYLNESGSMATGWVKDKGLWYYLNESGSMATGWVKD
                     KGLWYYLNESGSMATGWVKDKGLWYYLNESGSMATGWVTVSGKWYYTYNSGDLLVNTT
                     TPDGYRVNANGEWVG"
     gene            complement(3060..3209)
                     /gene="rpmG"
                     /locus_tag="VW411_001465"
     CDS             complement(3060..3209)
                     /gene="rpmG"
                     /locus_tag="VW411_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9794182.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(3225..3407)
                     /gene="rpmF"
                     /locus_tag="VW411_001466"
     CDS             complement(3225..3407)
                     /gene="rpmF"
                     /locus_tag="VW411_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEU9794183.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKITKAASAE"
     gene            complement(3559..3759)
                     /locus_tag="VW411_001467"
     CDS             complement(3559..3759)
                     /locus_tag="VW411_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9794184.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            complement(3762..4769)
                     /locus_tag="VW411_001468"
     CDS             complement(3762..4769)
                     /locus_tag="VW411_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU9794185.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKQISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            4961..6991
                     /locus_tag="VW411_001469"
     CDS             4961..6991
                     /locus_tag="VW411_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEU9794186.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NSHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            6995..7279
                     /locus_tag="VW411_001470"
     CDS             6995..7279
                     /locus_tag="VW411_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9794187.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            7292..8638
                     /locus_tag="VW411_001471"
     CDS             7292..8638
                     /locus_tag="VW411_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9794188.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFVGGLVSMVIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSATEE"
     gene            8642..9499
                     /locus_tag="VW411_001472"
     CDS             8642..9499
                     /locus_tag="VW411_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU9794189.1"
                     /translation="MILSKNREDGLRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEEKQMLTSVVER
                     LSQELEETE"
     gene            9496..10428
                     /locus_tag="VW411_001473"
     CDS             9496..10428
                     /locus_tag="VW411_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEU9794190.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSDSDIVVLEADLSSSMATNNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            10661..12364
                     /gene="ilvD"
                     /locus_tag="VW411_001474"
     CDS             10661..12364
                     /gene="ilvD"
                     /locus_tag="VW411_001474"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU9794191.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(12570..12650)
                     /locus_tag="VW411_001475"
                     /pseudo
     CDS             complement(12570..12650)
                     /locus_tag="VW411_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(12708..13049)
                     /locus_tag="VW411_001476"
     CDS             complement(12708..13049)
                     /locus_tag="VW411_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU9794192.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            13173..14023
                     /gene="rgg"
                     /locus_tag="VW411_001477"
                     /pseudo
     CDS             13173..14023
                     /gene="rgg"
                     /locus_tag="VW411_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398326.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes.; frameshifted;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
     gene            14214..15461
                     /locus_tag="VW411_001478"
     CDS             14214..15461
                     /locus_tag="VW411_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEU9794193.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGVSIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTLFTVFAGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVSVIISSLTGNDYFGKQSLPRLMMWETVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNIQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
     gene            15539..16828
                     /locus_tag="VW411_001479"
     CDS             15539..16828
                     /locus_tag="VW411_001479"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEU9794194.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIAMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVTLPIENALDVYIA
                     VLGEGANIKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            16819..16899
                     /locus_tag="VW411_001480"
                     /pseudo
     CDS             16819..16899
                     /locus_tag="VW411_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            16888..>17023
                     /locus_tag="VW411_001481"
     CDS             16888..>17023
                     /locus_tag="VW411_001481"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794195.1"
                     /translation="MDRTDEVSSKHGFEVVDRTDEVSSKHGFEVVDRTDEVSSKHGFE
                     V"
BASE COUNT         5148 a         2869 c         3506 g         5500 t
ORIGIN      
        1 cttccgtcgc atcaatcatt accgtgtcct cagaactaag aggagttctt gaaatcgtaa
       61 caccactttg aacaagagtt acttcaaccc attggctccg acggagtaag ttgctttcgt
      121 gaacaccaaa atcagccgca atttcttcat aagtgcggta ttctaggctt aatttaggtt
      181 ttcgtccacc ttttgcgtgt ttaagttgat aagctgtttt taatacagct aacatctctt
      241 taaaagtcgt gcgctgaaca ccaacaagac gcttaaatcg tgtatcagtt aattgtttac
      301 ttgcttcata atttcgcagg gagtctattg actctttggt aggtgtcaat gtttttttca
      361 tctatcccga gaattatttt cccgccattt gtatttgcaa atgctgagta ggtttcccag
      421 aaagactctg gaagattgtt tttagctttt ttgtattcta aatcaacccc ttcaaatttt
      481 aagtccatat ttttccttta catctgtttt ttgtggttct ggtatttgtt caagttgagt
      541 gataatatag cgaattgaat ttcgagagtt tttactcagt taatttcttt tttaacctac
      601 tttaatttct tttttaacac gggttaaaaa agaaattaaa gtgggttaat ttttcttgac
      661 ttaaaattta aaaatgatat actgtgttta tggagagatg acaatttata aaatgtacta
      721 ctctattcta ctagataatg attgaataaa actttttata acaacggcta gcaaagttat
      781 aattttatat ctattagaaa ataaatatgt aaggaaattt aaatgaaaaa aactacaata
      841 ttatcattaa ctacagctgc ggttatttta gcagcatatg tccctaatga accaatccta
      901 gcagatactc ctagttcgga agtaatcaaa gagactaaag ttggaagtat tattcaacaa
      961 aataatatca aatataaggt tctaactgta gaaggtaaca taggaactgt tcaagtgggt
     1021 aatggagtta ctcctgtaga gtttgaagct ggtcaagatg gaaaaccatt cacgattcct
     1081 acaaaaatca cagtaggtga taaagtattt accgttactg aagtagctag tcaagctttt
     1141 agttattatc cagatgaaac aggtagaatt gtctactatc ctagctctat tactatccca
     1201 tcaagcataa aaaaaataca aaaaaaaggc ttccatggaa gtaaagctaa aactattatt
     1261 tttgacaaag gcagtcagct ggagaaaatt gaagatagag cttttgattt ttctgaatta
     1321 gaagagattg aattgcctgc atctctagaa tatattggaa caagtgcatt ttcttttagt
     1381 caaaaattga aaaagctaac cttttcctca agttcaaaat tagaattaat atcacatgag
     1441 gcttttgcta atttatcaaa tttagagaaa ctaacattac caaaatcggt taaaacatta
     1501 ggaagtaatc tatttagact cactactagc ttaaaacatg ttgatgttga agaaggaaat
     1561 gaatcgtttg cctcagttga tggtgttttg ttttcaaaag ataaaaccca attaatttat
     1621 tatccaagtc aaaaaaatga cgaaagttat aaaacgccta aggagacaaa agaacttgca
     1681 tcatattcgt ttaataaaaa ttcttacttg aaaaaactcg aattgaatga aggtttagaa
     1741 aaaatcggta cttttgcatt tgcagatgcg attaaacttg aagaaattag cttaccaaat
     1801 agtttagaaa ctattgaacg tttagccttt tacggtaatt tagaattaaa agaacttata
     1861 ttaccagata atgttaaaaa ttttggtaaa cacgttatga acggtttacc aaaattaaaa
     1921 agtttaacaa ttggtaataa tatcaactca ttgccgtcct tcttcctaag tggcgtctta
     1981 gattcattaa aggaaattca tattaagaat aaaagtacag agttttctgt gaaaaaagat
     2041 acatttgcaa ttcctgaaac tgttaagttc tatgtaacat cagaacatat aaaagatgtt
     2101 cttaaatcaa atttatctac tagtaatgat atcattgttg aaaaagtaga taatataaaa
     2161 caagaaactg atgtagctaa acctaaaaag aattctaatc agggagtagt tggttgggtt
     2221 aaagacaaag gtttatggta ttacttaaac gaatcaggtt caatggctac tggttgggtt
     2281 aaagacaaag gtttatggta ttacttaaac gaatcaggtt caatggctac tggttgggtt
     2341 aaagacaaag gcttatggta ctacttaaat gaatcaggtt caatggctac tggttgggtt
     2401 aaagacaaag gcttatggta ttacttaaat gaatcaggtt caatggctac tggttgggtt
     2461 acagtttctg gtaaatggta ctatacctat aattcaggag atttattagt aaacacgact
     2521 acacccgatg gctatcgagt caatgctaac ggtgagtggg taggataggt atccattagc
     2581 taaatctaat cattctgaac taaaaaatct tcttacatat gtaggaagat tttttggatt
     2641 ctcaaaacta gtttaaaaga ctacgctatt taaaccaatt ttaagaatgg atatataagg
     2701 atattcagaa atctagttgg tcaacttttg tattttgaat ataccttaga tggtgacgat
     2761 gtcttggtat atctggtact cacttacata ttctaactta ttttaactaa gatacaactg
     2821 tagttggtga atttgctaca aatagtgtag aggcgttatt tgaaaaatat acaaaaaagg
     2881 aaccccaact tacatttgag ttcctcttgc ttttaattgt ccgaatccgt attcttattt
     2941 caaaaaaggt agtgaaaaat gtagtcattc gaatgatttc ccttacaatg tagtgaaatt
     3001 aacaaaaaga aaaaacgctg atcaagcgtt tttcttctgt attgattcgt attgaatgct
     3061 tatttaacct ctgtaaacac aacgtgtttg cgaagttttg gtgagtattt cttcaattga
     3121 aggcggtctg gagtgttacg tttgttttta gaagtaaggt acaagcgttc accagattct
     3181 ttgtgttcaa gtgtaatatt tacgcgcatg gtatctccct tctattattc agctgatgca
     3241 gctttagtga ttttacgtcc tttgtagtat cctttaagtg atacacggtg agaacgtgag
     3301 taatctccag tagtttcgtc aaagtttaca gatggagctg ttactttgta gtgtgtacga
     3361 cgtttgtttt tcttcgcttt tgaagtgcga cgtgcaggta ctgccatttt gatttctcct
     3421 ttaggtattt atattcgatt caatctactg attttcatca accatactag gataacacat
     3481 tttttttgta aagtaaagtt acttgacaaa aaaagatgaa aaaattctcc tatccgacac
     3541 ggcagacagg agaaggtgtt aaatatcaat ctcaaatggt tcgtcaatgg tttctgatac
     3601 gtattttccg tctttcttcc gttgcttgac acactctgtg aggagatatt cgatttgccc
     3661 attgactgaa cgaaagtcgt cttctgccca tgatgcgagt gcagcgtata actttgttga
     3721 gagtcgaagg gggatctgct tttttttagc ttcagccatc tttagtaaag gcttcctgtg
     3781 ttgacaattg gttgtgcatc atgattgcca caaagaacga caaggagatt tgaaaccatg
     3841 gcagcttttc gttcttcgtc aagttctacc aattcccctt cattgagccg ttctagtgcc
     3901 atttcaacca ttcctacagc accatctaca atcatcttcc gtgcatcaat aatggcagat
     3961 gcttgttggc gttgaagcat aacggcagca atttctggag cataagctag gtaagtgata
     4021 cgtgcttcaa ggatttccaa gccagcatcc tcaacacgac tttggatttc ttcacgaata
     4081 cggttagcaa caatttcgct agagccacgg agactacctt catctgcttg cccatcaccc
     4141 gtagtatcca cattaggaga cacatcgtaa ggatagatgc ggacaatatt acggagggcg
     4201 ctatcacatt gcaatgaaag atattctttg tagttatcaa cgttgaagac tgccttagcg
     4261 gtatcgacaa ctctccaagt taccgcgata ccgatttcta cagggtttcc taagcaatca
     4321 ttgatttttt gacgagaatt gctcaaggtc atgactttga gggaaatctg tttcttgcca
     4381 atttcaagat ttacatcatt gtcatttgat gatttagctc ctaaaaaagg agattttgtg
     4441 ctaacatcac cactttgtcc aagtcgagtg tggtttgcag ggttgactgc tacgctgaag
     4501 ggattgacaa agtaaaagcc aggttctttg atggtacctg tatagttacc aaagagtgtc
     4561 agaaccagag cttcttgagg tttgacaact tttaaaccag catgagctag acctgcaatt
     4621 acgattagta aaggtccgat aataattcca aaaatgtttt ccgaaccaac ccccattata
     4681 aatataaaga taccaagtac gattgtcaac tcaatgagaa tcaatgcgaa tacaccattt
     4741 ggttttgaat tgattagttt ttcagtcata agaatgacct cctgttattt gatatctaaa
     4801 tgatatcact ttaatttata aagtcaatag aaagttgaaa aataatcact aaaaactttc
     4861 agcccaataa tttcttttaa aaggtgagat tatattgtgc tcaaatactc ttttattctt
     4921 ataattttta cactataatt gtagttagaa aggaagagaa atgtttgatt atagagcact
     4981 agttattttg atgactttga tggatcattg tgagctatca ttatatgaat tatctgttaa
     5041 ggtgagctta cctataaagg aagtcaaaga aggaatagat tatttagtgc cttatctagc
     5101 taataaaggg atagtgctgg ataaaaagca aggtcgctat agtttatcaa atcgtacgaa
     5161 gcagtctttg acagatatta ttaagtcgga tgaattggtt ttaccaaagt cgactcgctt
     5221 agctttaatc tatctttaca ctttttgccg attagatttt atatcgaata gtcattacca
     5281 agactttttg aaagtaagta agaatacaac cttatctgat attcaatcat taagaaagat
     5341 tatgttagat aatgatttgg agctagggta cagtagagca aaaggttata ctttacacgg
     5401 ttcagagtgg aataagcacc gcttagcttt tcaaatggtt agtgagttgc tggaatcctc
     5461 catagggatt tggggtttgg attatgtttt atccagttgg gggtattcat taacttatga
     5521 tttgattgat caggtagtta aagattatta tgagaagcta cagctagtac ctattgttaa
     5581 tcaattaaaa gtttaccttt tcggtttagt attcattctg tgtaggtatc aaagggatgt
     5641 tgaaagagta tgtctttcag agacattagt atctcctgtt attcaagata taaccactat
     5701 tttattggat acagtggttg atttgggaat tatagatacg gtgttttctg aggatgatta
     5761 tcgctatatc accgttttat tatcaagttg ctttgaaggt gaagtagatg ttgctccggt
     5821 ttactttaat caattaacag aggctattat aagtagaatg gaagatattt ctttgttaca
     5881 ttttaaacaa agggaaggat tgagagaaaa tcttcgccgc caccttatac cggcttacta
     5941 tagattaaag ttcggcctac ctagttcaaa tgaatatgta ttgcatgtta aagaacatta
     6001 tcctgattta tttgaattag tcaaagattc tttgatgccc ttgatggacg ctattgacaa
     6061 tcccatacct gatagtgaaa cagcatattt tgttatccat ttcggaggtt atttaaagaa
     6121 agcagatact ttgcctcaaa aatggtataa agcagcaatc atttgtccta atggtgttag
     6181 ttcttcattg atgttaaaag agaatctatt agcattattt cctcaaattg agtttatagg
     6241 aacctcaaag attgatgatt tacaggtgaa agctagtagt gactatgata tggttttttc
     6301 taccataaag gtggagacag aaaagccaaa ttatctagtt tcggttatga tgactgaaga
     6361 gcaagcaata caactagtag aactagtgtt aaaagatttt ccgaatttag agtatggaga
     6421 ttttgagatt gaacaaatcc tgaatattgt caaaagatat ggaattatta cacaagaatt
     6481 ggaattaaga ttggcgttaa agaattatct ttaccaaaaa aatgatagaa aggaaattgt
     6541 accagtgcta gaacaactta ttaccaaaga aacctatcag gttagttcgc aaaaattagg
     6601 atggaaggag gcgattcgtt tagcagctaa accgcttttg gatcaagata agataactga
     6661 gaactaccct gaggcaatga ttcaaaaagt agaagagttt ggacctttta ttaatttagg
     6721 gaagggagta gcaattcctc acgctcggcc agatgaaggt gtgaatgaaa taggaatgtc
     6781 aatgttagtt ttggaagaac cgatttattt attggataat ccagaacaag aggtaagatt
     6841 gttgatttgt attgcagcca ttgataatga gagtcattta aaggctttgt cacatttaac
     6901 aacaatttta agagataaaa atcatgttca aactttaata tcatcaaaaa actatgatga
     6961 cattaaaatg ataattaaac aggaggatta gataatgtta aaaattggta cagcttgtgg
     7021 ttcaggatta ggttcaagtt ttatggtaca gatgaatatt gaatctgtat tgagtgattt
     7081 gaatgtttcg gatgtagaag ttgaacatta tgatttaggt ggagcagatc caaatgcagc
     7141 tgatatttgg attgttggtc gtgatctagc tgattcagct agtcatcttg gagatgttcg
     7201 tatcttaaat agtattattg atatggatga actacgagaa ttaattacta aactttgtga
     7261 agaaaaagga cttatatagg aggctagcgt catgatgaag ttcatattgg atattgttag
     7321 tacaccagct attttagtag ctttaattgc aatcttagga ttagttcttc agaagaagaa
     7381 attacctgat attattaaag gtggaattaa gacctttgtt ggtttcttag ttgtatctgg
     7441 tggtgcagga attgtacaaa attctttaaa tccatttggt accatgtttg agcatgcttt
     7501 tcatttatct ggcgttgtgc cgaataatga agcaattgta gctgtagctt taacaacata
     7561 tggctcagct actgcaatga ttatgtttgc aggcatggtg ttcaatatct taatcgctcg
     7621 ttttactcga tttaaatata tttttttaac agggcaccac actctatata tggcatgtat
     7681 gattgcggtc attttatcag ttgctggctt tactagcttg cctctcatct tactaggagg
     7741 attagcactc ggtattatta tgagtatttc cccagcattt gtgcaaaaat atatggttca
     7801 attaactgga aatgacaagg tagctttagg tcatttcagt tctttgggat attggttgag
     7861 tggttttact ggtagcctta tcggtgacaa atcaaaatca acagaggaca ttaaatttcc
     7921 aaagagttta gcttttttac gtgatagtac tgttagtatt actttatcca tggcagttat
     7981 ttacattatt gtagctatct ttgcagggtc agaatatata gaaaaagaaa tcagtagtgg
     8041 tacaagtggt ctagtttatg ctttacaatt agcaggtcaa tttgcagcag gggtatttgt
     8101 tattttagca ggtgttcgcc ttattttggg cgaaattgtt ccagccttta aaggtatttc
     8161 agagcgtctt gtacctaatt caaaacctgc tttggattgt ccgattgttt atacttatgc
     8221 acccaatgca gttctaattg gatttatctc tagttttgtt ggtggtttag taagtatggt
     8281 aattatgatt gcttcaggaa cggttgttat cttaccaggt gttgtgcctc atttcttctg
     8341 tggagcgact gcaggtgtca ttgggaatgc atctggtggt gttcgtggag ccactattgg
     8401 agcattttta caaggtattt taatcagttt tcttccagtc tttttaatgc cagttttggg
     8461 aggacttggt ttccaaggat caactttctc agatgcagat tttggtctat caggaattat
     8521 tttaggaatg ttaaatcaat ttggctcaca agcaggcatt gtgattggtc ttgttcttat
     8581 tctagcagtt atgtttggag tatcctttat taaaaagcca tctgcaacgg aggaataagg
     8641 gatgatttta agtaaaaata gagaagatgg gttaagaaaa tttgcgacta acatccgatt
     8701 aaatactctt agaacattga atcatcttgg attcggacat tacggaggaa gtctgtctat
     8761 cgtagaagtt ttagcggtgc tttatggtga aataatgcca atgactccag aaatatttgc
     8821 agcacgagat agagattatt tcatattatc aaaaggtcac ggaggaccag ctctatacag
     8881 tacactctat ttgaatggtt tctttgacaa agaattctta tattctttaa atacaaatgg
     8941 aaccaaatta ccgtctcatc ctgatagaaa tctaacgcca ggcatagata tgacaacggg
     9001 ctctttagga caaggaatta gtgttgcaac tggacttgca tatggtcaga gaataagaaa
     9061 gagtcccttt tatacttacg ctattgttgg agatggtgag ttaaatgagg gacaatgttg
     9121 ggaggctata cagtttgctt ctcatcaaca gttatccaac ttaattgtat ttgttgatga
     9181 taacaaaaaa caattagatg gttttacaaa ggatatttgt aatccaggtg atttcgtaga
     9241 aaaattttca gcatttggat ttgaatccat tagggtcaag ggttcagata ttagagaaat
     9301 ttatgaaggg attgtccaat taaaacagtc aaataattca tcacctaagt gcattgtatt
     9361 agatactatt aaaggtcaag gggttcaaga gctggaagaa atgaaatcca atcatcatct
     9421 tcgccctact gtagaggaga aacaaatgtt aacttcagtt gtagaaagat taagtcagga
     9481 attggaggaa acagaatgat gagaagtacg aaagaattac ggcatgtata tagagacttc
     9541 cttttagagg ctaatcaaag tgattctgat atagtagtct tagaagccga tttgtcaagt
     9601 tcgatggcta ctaataatct tgaaaaggac tttggagacc gttatgtgaa tgttgggatt
     9661 atggaagcag aaatggtcgg ccttgcagca ggcttgtcta ttcaggggtt tagaccttat
     9721 cttcatacat ttggtccttt tgcttcacga agagtatttg atcaattatt tatttctctt
     9781 ggatacgcac aattggatgc cactgtgatt ggatcagatg caggagtaac ggcagagatg
     9841 aatggtggaa cacatatgcc atttgaagaa attggattgt tacgtttaat tcctaaatca
     9901 atcattttcg aagcaactga tgatatccaa tttcgtgaaa tattgaacca gacattagac
     9961 ttaaaaggac taaaatatat tcgaacaatt agaaaagctc cagttcctgt gtatcaaggt
    10021 ggagaagatt tttctaaagg ctacattgag ttaaggcatg gtgaagatct tgtaatcgtt
    10081 gcttctggca taatggttgc tccaagtatt cgagttgcag atgaactgtc taaattaggt
    10141 tattcagtag gtgtgataga tttatttaga atcaaaccga taccagaaca gataaaaaca
    10201 atgttaagtg gaaaaactat atttactgta gaaaaccaca atcagatagg tggaattggc
    10261 agtgctttat gtgaattatt ctctgatgat ggaataacaa aaattcatcg gatgggtgtt
    10321 caagaaagat tcggtcaagt agggaaaatg gattatcttc ttaatgagta tggtttgagt
    10381 gaatcgaata taaaagagaa ggttctagaa atttataatg caagatagat gttaaataca
    10441 cttatttgaa agaatttctg taaaaattac agtacgttat ttaaagaaag cggattaaaa
    10501 agcatattct ggtcatcaga aagtgcacta atccgctttg ttgtttttaa ttttaaatat
    10561 ttcacatgtg tatagaattt tctgaaaatt caagaatttt attctgttct tcatgcgaaa
    10621 attgtgctat aatagtaaaa actgaaatgg gagggataag atgactgaat tagataaacg
    10681 tcaccgcagt agcatttatg acagcatggt taaatcacct aaccgtgcta tgcttcgtgc
    10741 gactggtatg acagataagg actttgaaac atcgattgtg ggagtgattt cgacttgggc
    10801 ggaaaataca ccatgtaaca ttcacttgca tgatttcggg aaactggcta aagaaggtgt
    10861 caaatctgca ggcgcttggc ctgtacagtt tggaaccatt accgtagcgg acgggatcgc
    10921 tatgggaacg cctggtatgc gtttctctct aacatctcgt gacatcatcg cggactccat
    10981 cgaggcggct atgagtggtc acaacgtgga tgccttcgtc gctatcggtg gctgtgacaa
    11041 gaacatgcct ggatctatga ttgctattgc taatatggat atcccagcta ttttcgccta
    11101 tggtggaact attgcaccgg gaaatcttga tggtaaagat atcgacttgg tttctgtctt
    11161 tgaaggtatc ggaaaatgga accacggtga catgacagct gaggacgtga aacgtcttga
    11221 atgtaatgcc tgccctggcc ctggtggttg tggtggtatg tatactgcta ataccatggc
    11281 aactgctatc gaagttctag ggatgagttt gccagggtca tcctctcacc cagctgaatc
    11341 agctgataag aaagaagata tcgaagcagc aggacgtgct gttgttaaga tgttggaact
    11401 tggtctcaaa ccatcagata tcttgactcg tgaagccttt gaagatgcta tcactgtaac
    11461 gatggctctc ggtggttcta caaacgccac tcttcacttg ctcgccattg cccatgccgc
    11521 aaatgttgac ttgtcacttg aggacttcaa tacgattcaa gaacgtgtgc ctcacttggc
    11581 cgacttgaaa ccatctggtc agtatgtctt ccaagacctc tacgaagtcg gtggtgtccc
    11641 tgcggttatg aagtatttgt tggcaaatgg tttccttcac ggagatcgca tcacatgtac
    11701 tggtaagact gtagctgaaa acttggctga ctttgcagac ttgactccag gccaaaaagt
    11761 tatcatgcca cttgaaaatc caaaacgtgc ggatggtccg cttatcatct tgaacgggaa
    11821 ccttgctcct gacggtgcag ttgccaaggt atcaggtgtt aaagtgcgtc gtcacgttgg
    11881 gccagctaag gtctttgact cagaagaaga tgcgattcag gccgttctga cagatgaaat
    11941 cgttgatggc gatgtagtcg ttgttcgttt tgttggacct aaaggtggtc ctggtatgcc
    12001 tgagatgcta tcactttctt caatgattgt tggtaaaggt cagggagata aggtggccct
    12061 cttgacggac ggacgtttct ctggtggtac ttatggtctg gttgttggac atatcgctcc
    12121 tgaagctcag gatggtggac caattgccta tctccgtacc ggcgatatcg ttacggttga
    12181 ccaagatacc aaagaaattt ctatggccgt atccgaagaa gaacttgaaa aacgcaaggc
    12241 agaaacaacc ttgccaccac tttacagccg tggtgtcctc ggtaaatatg cccacatcgt
    12301 atcatctgct tcacgcggag ccgtgacaga cttctggaat atggacaagt caggtaaaaa
    12361 ataaactcat actcgaaaat ctcttcaaat cacgtcaacg tcgccttgcc gtaggtatgg
    12421 ttactgactt cgtcagttct atccacaacc tcaaaacagt gttttgggct gacttcgtca
    12481 gttctatcca caacctcaaa acagtgtttt gagctgactt cgtcagttct atccacaacc
    12541 tcaaaacagt gttttgagct gacttcgtca gttctatcca taacttcaaa acagtgtttt
    12601 gagcaacctg cggctagttt tctagtttgc cctttgattt tcattgagta tcaaaaagca
    12661 agccattttc ggcttgcttt tttcatcttt aaaagtgatt ggtctgctta acgaggtggt
    12721 agtccaaggg caatacggcc atagcgactg attcgtgtga ttttccaagc aggcgaccag
    12781 gtaacttcaa ccttgatatc tttgataccc tcgatttgtt tcagacctgc cacgatttca
    12841 ataggcaggc tttcggcgca atcacaggca gtatcggtga aggtcatgac aatcttgcag
    12901 agccccgttt catccagatt gatttcataa atcagaccta gattgtaaac atccaattcc
    12961 acatctgtat caaaaacttt ctctagtttt tcgataattt ggtcttgcaa ggccaaagca
    13021 cggtcattga ttttgatatc gtctctcata gcagtccctc cacgtgataa atatcctcta
    13081 ttttctcata attctgacaa tttggcaagt ttttgatgaa ttttctgaaa aatatgataa
    13141 aatgaagaaa atacggaatc aaggggaagt ctatggagca gattggaaaa gtctttagac
    13201 aattacgaga gtcaagaaat atctcactga gacaagcaac cggaggacaa ttttcgccgt
    13261 ctatgttgtc ccgttttgaa acaggtcaga gtgagctttc ggtggaaaag tttctatttg
    13321 ctctggaaaa tatatctgcc agtgtggagg agatcctctt tctggcaaga ggtttccagt
    13381 atgatacaga ttctgagttg agaaaagaaa ttacagatgt cttggaacca aagaatatag
    13441 cacctcttga ggacttatat cgcagggagt ataaaagcat gcccattctc acaacaaaca
    13501 gaaacatatt ctaaatgcca ttattatcaa gtcttatatg aagagcatag atgaaagggt
    13561 agacttaaca gcagaggaag gaaaagtcct tcatgattat ttgttttcta ccgagatctg
    13621 gggaatctat gaactcaatt tattttcagt cagttctcca tttttatctg tttctctttt
    13681 tactagatat gtacgagaaa tggtacgtaa atctgatttt ctaatggaaa tgtctggcaa
    13741 tcgaaacctt tttcatacta tactattgaa tggcttttta gctagtattg agtgtgaaga
    13801 atttaccaat gcctattatt ttaaacgtgt tatcgaagag catttctaca aggaaaatga
    13861 gacctatttc cgaattgttt atctgtgggc agaaggtctc cttgatagca agcaaggtag
    13921 agtcaaggaa ggccagaaaa agatggagga tgctgtccgt atttttgaga tgcttggctg
    13981 taataaatct gccgaatact atagaaaaac tcccgattct tgaatatctg caaaccaaga
    14041 aaaaatttga agcgatagaa ctgttgccct ctctcaggtc attacaaaag ttctatcgtt
    14101 tttttgctca ttttgtttgt tgcatatatt caaaagaatc gtttcgcaaa ttcataagtg
    14161 ctatactaaa gctattcttg atgtagaact tagaacaagg agggagatta tctatgaaac
    14221 tattgtttag aaatccagct tatagaattt tgactttatc acgcttcttc aatgcttttg
    14281 gtgtttcgat tttcaacctg gtctttatcg tctatgcatc gaccttgtca caagcatctt
    14341 ttgctgttgc tatggcgaat attgtcatga ttcttccgac cctctttaca gtttttgcag
    14401 ggattcgggc agattacacg agggacaagg tcaaatggat ggtctatagc ggtttgtttc
    14461 aggcggttct attttttcta gcagccctag ttgttcagca agctagtctc tttgcctttt
    14521 ctagcctgtg tttgatcaat gtcattagtg atatcatcag tgattttgca ggtggcctgc
    14581 gtatgcctct tattaaggaa aaagtagctg aagatgatct gatggaggct tattcttttt
    14641 cacagttcat cacctatatt tcagctattg gtggtcaagc tttcggagtc tggctcttag
    14701 ctctatcggt caacaatttt tctctcgttg cgggaatcaa tgcctgcttt ttcctagtat
    14761 cagccactat cctcttttta ggaaaaagca aattgagcct gtcaatgtca tctgctgatg
    14821 gtgaaaactt aaaaaatgag aagctttcta tcaaagacca gttcctaaca atttaccgaa
    14881 atttacgtct cgtttttctt aaaagtggac agaaaaactt tggttttatg ctctttgctg
    14941 tcttgcttat taattccttg ggtggcgctt tgggtggaat ttataatatc ttctttttga
    15001 gccattctct cttgaatttt tcttatacag aggcactatt tatcaatcaa ttctgtgttt
    15061 tagtatcagt catcatcagt agccttacgg gcaatgatta ttttgggaag cagtccttgc
    15121 ctagattgat gatgtgggag accgtaggac ttagtctagt tggtttggct aatttattca
    15181 atcaagtcgt gcttggtttg ctatttctct tctttactct gtatgtgtct ggtaaagttc
    15241 aaccaaagat tagtgccatg ctcatgaaaa atctagctcc agaggttcta gctcgtacta
    15301 gtaatttttt aggtctattg tttaccttat ccatacctgt gggaacagct tgtttttcac
    15361 tagtagccgt atggaatata cagttgactt ggatgctatt tgttggtctt tccttgctag
    15421 ctattttttt gacaattctt aatctcaaaa atgatatcta aatcctaatt tttttcttac
    15481 tgttttaatc cctctattta tggtaaaata agactattaa gtttaaagag gattccctat
    15541 gaaattacaa aaaccaaaag gaacgcagga tattttacct gctgagtctg ctaagtggca
    15601 gtacgttgag ggctttgccc gtgagatttt caaacgctac aactatgcag aagtgcgcac
    15661 gcctattttt gagcattacg aggttatcag tcgctctgtc ggagatacaa cggatatcgt
    15721 aaccaaggaa atgtacgatt tttatgacaa gggtgaccgt catattaccc tccgtccaga
    15781 aggaactgca cccgttgtcc gttcctatgt ggaaaataaa cttttcgccc cagaagtgca
    15841 aaagccaagc aagttctact acatgggacc tatgttccgt tatgagcgtc cacaggcagg
    15901 gcgcttgcgc caattccacc agattggtgt tgagtgtttt ggctctagca atccagctac
    15961 cgatgtggaa acaatcgcta tggcagccca ttttttgaag gaaatcggta ttcaaggtgt
    16021 caaattgcac ctcaacactc ttggaaatcc tgagagccgt gcagcctacc gccaagcctt
    16081 gattgactat ttgacaccgc tcaaggagac cttgtctaag gatagccaac gtcgcttgga
    16141 ggaaaatcct cttcgtgtct tggactctaa ggaaaaagaa gacaaggtgg cagtagagaa
    16201 tgcgccgtct atcttggact ttcttgatga agaaagccaa gctcattttg atgctgtgcg
    16261 tcagatgttg gaaaatcttg gagtagatta catcatcgat accaatatgg tgcgtggtct
    16321 ggactactac aaccacacca ttttcgagtt tatcacagag attgagggca atgacctgac
    16381 cgtctgtgcg ggtggtcgct acgatggttt ggttgcttac tttggaggcc ctgaaactgc
    16441 tggatttggt tttggacttg gtgtagagcg cctgcttctc atccttgaaa agcaaggtgt
    16501 gaccctccct atcgaaaacg ccctagatgt ctatatcgca gtcttgggcg aaggggcaaa
    16561 tatcaaggcc ttggaattgg tacaggctct tcgccaacaa ggtttcaaag cagagcgtga
    16621 ttacctcaac cgtaaactaa aagctcagtt caagtcagcc gatgtctttg cggctaagac
    16681 cctcatcacc ctaggagaga gcgaagtcga aagcggacaa gtgacggtca agaacaacca
    16741 aacccgagaa gaagtgcaag tgtcacttga gacaatcagc caaaacttct cagaaatctt
    16801 tgaaaaacta ggattttata ctcaataaaa atcaaagagc aaactaggaa gctagccgca
    16861 ggttgctcaa aacactgttt tgaggttgtg gatagaactg acgaagtcag ctcaaaacat
    16921 ggttttgagg ttgtggatag aactgacgaa gtcagctcaa aacatggttt tgaggttgtg
    16981 gatagaactg acgaagtcag ctcaaaacat ggttttgagg ttg
//
LOCUS       DAVKNL010000044        15997 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVKNL010000044 DAVKNL010000000
VERSION     DAVKNL010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15997
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.044"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            <1..209
                     /locus_tag="VW411_001482"
     CDS             <1..209
                     /locus_tag="VW411_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9794196.1"
                     /translation="RKSSKLKPLTAEDKAYNHALSKERSKVENIFAKVKTFKMFSTTY
                     RNHRKRFGLRMNLIAGIINHELGF"
     gene            complement(261..623)
                     /locus_tag="VW411_001483"
     CDS             complement(261..623)
                     /locus_tag="VW411_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794197.1"
                     /translation="MPKTKTLAELADVILWSFDFANDHAHAFFMDNVEWSHADSYFRS
                     FVSDDVEERYTENVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSV
                     GTSPEQYPDYDGFDYEEW"
     gene            complement(624..833)
                     /locus_tag="VW411_001484"
     CDS             complement(624..833)
                     /locus_tag="VW411_001484"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794198.1"
                     /translation="MKQKNYLVSNATVRQTYDKIAESECFLRAIGGIMLHLKLVKQEI
                     EAEKPASVEAWIISVKFKKGCYRHI"
     gene            complement(985..2865)
                     /locus_tag="VW411_001485"
     CDS             complement(985..2865)
                     /locus_tag="VW411_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEU9794199.1"
                     /translation="MVRFTGLSLKQTQAIEVLKGHISLPDVEVAVTQSDQASISIEGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEILALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDVTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDGLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDIL
                     CPILDQHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(2859..3728)
                     /locus_tag="VW411_001486"
     CDS             complement(2859..3728)
                     /locus_tag="VW411_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9794200.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNILCQEAIERMNRNLAQGLLN
                     IQYLIDPGVISLGGSISQNPDFIQGVKKAVEDFVDAYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(3811..6456)
                     /locus_tag="VW411_001487"
     CDS             complement(3811..6456)
                     /locus_tag="VW411_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEU9794201.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYNLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTACYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFALECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMCSENVRVPESQHLFLDLLE
                     RPYPVHSGLLAPQEIRTEFIKKEEI"
     gene            complement(6547..7827)
                     /locus_tag="VW411_001488"
     CDS             complement(6547..7827)
                     /locus_tag="VW411_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEU9794202.1"
                     /translation="MVYSKEIVREWLDEVAERAKDYPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDEIF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIKNYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            7999..10083
                     /locus_tag="VW411_001489"
     CDS             7999..10083
                     /locus_tag="VW411_001489"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEU9794203.1"
                     /translation="MKPLLETIDTRFGTTNKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLNQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKSSAQVDWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEINESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMTPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            complement(10123..11802)
                     /locus_tag="VW411_001490"
     CDS             complement(10123..11802)
                     /locus_tag="VW411_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEU9794204.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWASDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGSGHTVGYKRLLRGAVVEAQKIRVVITESQALPLLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGENAYFTVKRRECSGPLEAKISIQPGTGVHGVAYQ
                     DEIQVLAFQTGETEKSLTLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            12557..13786
                     /gene="arcA"
                     /locus_tag="VW411_001491"
     CDS             12557..13786
                     /gene="arcA"
                     /locus_tag="VW411_001491"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEU9794205.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESEYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            13844..14860
                     /gene="argF"
                     /locus_tag="VW411_001492"
     CDS             13844..14860
                     /gene="argF"
                     /locus_tag="VW411_001492"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU9794206.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            15025..15972
                     /gene="arcC"
                     /locus_tag="VW411_001493"
     CDS             15025..15972
                     /gene="arcC"
                     /locus_tag="VW411_001493"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEU9794207.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
BASE COUNT         4538 a         3693 c         3252 g         4514 t
ORIGIN      
        1 cacgtaaatc cagcaaactc aagccactaa cagctgaaga taaagcctat aaccatgcgc
       61 tatctaagga gagaagcaag gttgagaaca tctttgccaa agtaaaaacg tttaaaatgt
      121 tttcaacaac ctatcgaaat catcgtaaac gcttcggatt acgaatgaat ttgattgctg
      181 gtattatcaa tcatgaacta ggattctagt tttgcaggaa gtctattgaa atactaagcc
      241 tacacagact gatttcaatt ttaccattct tcatagtcaa aaccatcata atctggatat
      301 tgttctggcg acgttccaac cgaacgtacg agataagctt cttcgtcctc tgtctcgatt
      361 tctctcagca cttggcattc aaaacgccat tcatcaccga agtcgaaaat aaacttaaat
      421 ttttgtttga cacttaggct atccagatag acattttctg tgtaacgttc ttcaacatcg
      481 tcactaacaa agctacgaaa gtaagaatct gcatgactcc actcaacatt atccatgaaa
      541 aatgcgtgag catgatcatt tgcaaaatca aaactccata aaatcacatc tgctagttcc
      601 gctaacgttt ttgtttttgg aatctatata tgtcggtagc aacctttttt aaatttgacg
      661 gaaatgatcc aagcttctac agatgctggc ttttcagctt ctatttcttg ttttactaat
      721 tttaagtgta gcataatacc tccaattgct cttaggaaac attcactttc tgctatttta
      781 tcataagttt gtctaactgt agcatttgaa accagataat tcttctgttt cacgcgaaac
      841 accctgcgcc aagcaaggtg ttcttgtttt tatactcaat gaaaatcaaa gagcaaacta
      901 ggaagctagc tgcaggttgc tcaaaacact gttttgaggt tgcagatgga agctgacgtg
      961 gtttgaagag attttcgaag aatattaagt cgtataaatc gtcgacgctg tcgcaatggt
     1021 atgccactgg ttggctgtag ttgctgcgaa atccttgtct gcgtagaagt cagtaaatgg
     1081 taggatttca acttccagct cgtcgatgcg gtcaagctga ccagccagat aaacctcgat
     1141 acggctttct gctcgtttga tgcgttgcaa gagtccgccc atacggatgt caactgtatc
     1201 caaaccaaag accttgtttt ctttcagcca ttggtggcta aagagggcat ggaagtcttc
     1261 aatttggctt ctaagttctg gtaattcttg tctggcgatt tgttgtaaac tttctttatc
     1321 atccgcttgg taggcctgac gaatgcgtcg tcccacatct actttgctac ttaaaatagc
     1381 attcaactgg gcctgagttt caaagagata ggcatagttt ccagcttttt ctttaatgtt
     1441 agcaagcgtc tcagcagcct gagcgaagtg cggtttgtcc tgttcaggtg tcatgtgttg
     1501 atcaagaatc ggacaaagaa tatcctgata aaaaacatag cggttgggat tgataccgct
     1561 gagattgcct ggtaggtctg gtaagaggtt ggcaaggtca atctgcataa aatcctcaac
     1621 cgttagacca gtattggtct tgaagtgcgc agacaaacca tctaggtcat tgcgatagct
     1681 gagttctgcc cagatttgca agcttggtag gatagagaac tgggcagttt caccaccatt
     1741 gtctccccaa cccgttacga tgacttcttt aatctgattg gcacggcagg ctttattagc
     1801 ctcgatagcc actagacggc taaaatggtt gtgaggtgta aagccaatcc acttccaagc
     1861 tccccctgca aatgcaaggt catggctaat cttgtgatga ttgcggaaat tacggttgta
     1921 tttttcctcg ctatcctgat aataatccca gtaaaccaga gtcacacggt ctttgagacg
     1981 gtctagatag acacgagttt cctctggaat ttccacatca cggtcgtact ggccatccgc
     2041 tgacatgagt ttgaagaaca tatcactcca catctggcag tggaaaccat atttgtcagc
     2101 aatatccagc acgcgctcca agtgttggca catgaggaga ctacgatcca caacaccgtt
     2161 cagaatcagg tagcgtccca aaccaaccaa gtgggcttcg tccatcccga tattgacctt
     2221 gcgagtcttc agtttagaca acgtggcaaa catgccatca atcaagtcat aaaccttttc
     2281 ttcgccaatg agaagaatgt cctctacatc acggagctcc tgcacttcct tgacacccca
     2341 tttgacaaag gccgacaagt gggccaaggt ctggatgcat ggtacaaagg tcacgtcaaa
     2401 ctgttgggca taggcttcga tttcctgcaa ctcctctgct gaataagctc cacggaaata
     2461 gccaaagtaa ggctgccctt caatctggta agtgtcttcc atgtaaagct caaaggttga
     2521 gtagcccatg agagccaata tctcaatcat ctgcttggca gaagccacat tcagcaccgc
     2581 atttcgagaa cagtcaacca tgtaagccaa atcttcgtaa gctgcttgtt cctcaatctc
     2641 tactttatca gcttctgcta gaactgttac caacaaggac aaggcacgat aaagttggtg
     2701 aggtttgcgg taggtcaatt gatagtgacc ttcctcaccc tcgatagaga tagatgcttg
     2761 gtcagactga gtgacagcca cttccacatc tggtagagaa atgtgacctt ttaaaacctc
     2821 aatagcttgc gtttgtttga gactaagtcc tgtaaatctt accattgctt ttcctcctgt
     2881 agccagttga caagagcacc gtagagattg gcatctgcgt gataggtgca ggcctggata
     2941 actggtgcga ccgtgtattc ttcgtaggca tcgacaaagt cttcaacagc cttcttgaca
     3001 ccttggataa aatctggatt ttgactgata gagccaccca gactgatgac acctggatcg
     3061 atcagatact ggatattgag caagccttgc gccagattgc ggttcatgcg ctcaatggct
     3121 tcttgacaaa ggatattacc agctgcggcc tcttggtaaa tcttgcgacc gtcccaatca
     3181 gtatgaccag atttttcaat cacgtatcgt accatattcc cagttgatgc tagttgcgac
     3241 cagttattaa gtttttcagc aggggcaagg gttgtcatgt agccaaattc tccacccaga
     3301 ccgtggcgac ctcgatgaag tctaccattg ataatcatgg ctccgccaat ccctgtccca
     3361 atcacgacac aggctgcatt ttcaagctct ggatgagcta gtagttcact gagtccaacg
     3421 cagttggcat cattttctaa atggacaggt agctgataag agctaagcgc ctcataccaa
     3481 gaaaagccat ggatgtaggg caccgcactg aagccatcaa tcacacctgt ctcttgattg
     3541 actgcacctg gaacgctcat agcaatccca ctgtaatcct gttctgacaa gcgttgatct
     3601 agccacgcta gtaaatcctc caagttttca ggcgttgaaa tacttgtctt atccagtatt
     3661 ttcccatcag gagtcagact ggcaaactta atcccagtcc ctccgatatc aatcgttgca
     3721 atggtcattg tttttaccat aaaaggggcg aatcagcagt tagttcttac tttttcgccc
     3781 ctcctttctt ttgatgttta ctttttgaaa ttaaatttct tcttttttga tgaattctgt
     3841 acgaatctct tgtggagcca atagtcctga atgaactggg tatggtcgtt caagtaggtc
     3901 aaggaagaga tgttgacttt ctggcacacg tacattttca ctacacatat tgtagtaacg
     3961 aagcacatag ccttcttcat tttcagctac cttaaaggct gttggacaaa cttgcggtat
     4021 gctgagaaca gaatggctca agaggctacc agtcgcaacc acgcttcctt cctgtctagc
     4081 aagctgaagg ctggtaaacg gtgtctgcaa ggctttggca cgacgatagg ctgagaagcg
     4141 ttcttgggct tggtggcatt caagtgcaaa ctcgacttca aactcccgca agcattgtgc
     4201 ttctggcgtt gggaagtagc cccagtcacc tagctcacct gatgcacgca aaatggtcac
     4261 ggcaatggtg ttatccccaa ggatttcgta ttcattcaat cccttgttgg atacagtcac
     4321 ccctttttca tcgtcataca gactgacaaa agcttgttgg tgttgaggat tttcagggtt
     4381 ttcccatgaa gcagctggtt tgtttggtcg tgtcaccacc tcatagatac tttcagaatc
     4441 attgcttgga cgcgtgttat gagtcttgac caagagacgg atacggtgat ccttggcagt
     4501 gttagtaaag cgagtcttga agcggatttg tggattgtca acgaagacag tcaactcagt
     4561 ttccagagga atgtttgtca attcttctga ccgtccagcc tcacgcttca taaactcgat
     4621 gataccttgt tgctcttctt ctagcttttc atccgcactg acaggcacgg tcaattcatg
     4681 tttgagcaag attttagcat agcaagctgt gttttccaag acctcgtggc ccttaagctc
     4741 tgcaaagatt ggctctgttc cttttggttg gaaatagata tactcgtttc cgatgtcccc
     4801 acggtcttca aagcggataa agtcttcata ggcttcgtga gttgtcttgt catagactgt
     4861 gatgttgtca tccacactca ccgttacgaa tggtgtatca atcactccgt tttggtaaat
     4921 accctcacgg tgttcttgtt ttccttccag caattggaag gttgtccaag aaagcggcgc
     4981 taggtgaact ggaatggtca cgcgcacttg acgagcaata cgagcttggc ggaacttgtc
     5041 ttttggtaaa ttatactcaa aattagctcc gaggtcttcg attgtagcct ctacaggacg
     5101 accatccaag tcctccacac ggtaacttgg caagataaga gcagccatct ttttgtagcc
     5161 ttctgttggg tgcaattcct tgaaatcaca agtcgccaca tcaatcactg tgctgacagt
     5221 atcgacctta tcatgcaagc ctgtgttaat gacagtaaag agatagtcac tttgagcctt
     5281 atccgtagca attttaccct tccactcgtt gagcaagtta cttttaacaa agtttcctac
     5341 ttggttgacc ttggcaaaac gcgtttccat ctcgcggtga acttcgtcca cgctacagcc
     5401 acagatacta tcatgtggcg cattctgcaa aagtgttttc caagcatagg tcaactggtc
     5461 cttgtggttg tgtccaccag tgataatagt caagggttct accacttgct ctaggaggtt
     5521 gctattttct tggaaggctt gttttaggta aatgcgggat gaagaagtgt tggcaagtgt
     5581 gtaccagcca tctgtttcct gactggtcaa ctcgcctgta acagttgata agtgttcagg
     5641 aagcgcacct tctacagctt gaacatattc atcaaaagaa ctatgaacaa agattacatc
     5701 cgggaagagt tcatttgcca cacgaatggc ttcgctcaga tttttctgta caggctggtg
     5761 gtcacagccg ttcatcatca accattggtt ggtcgaagcg taggcacgca catctgacaa
     5821 tttttgtttc cagaaggtca aggcctcatc tttgtcaact ggaatttcat tcccgttact
     5881 gtaccagttg gcaaagagaa tacctaaaac acgactacca tccacaccct gccagtacat
     5941 ttctgaaaac tgagacgtaa actgctcatc ttcaaggact tggttgtcaa atccaatcgg
     6001 cttcacacca cgaccaaagg ccgccacgtg aatgcctgat ttttgaagaa tttgaggcgc
     6061 ttgtcccata tttccaaagg tatctggaaa gtagccaatc tgggttgatt taccccattt
     6121 ggcagcttct tgttgaccaa tcaaggtatt gcggacattg gcttcactgg agatcaagta
     6181 gtcatcctgc aagatgtaaa agggaccaat tttaagtttg ccctcgtcaa tgtagcgttg
     6241 gaccttgtcg cgattttcag ggcgaatttg taagtagtca tcaaggacaa tagtttgtcc
     6301 atccaagtgg aaactcttga actcagggtc attttcaaag agatcaaaga gattgtcaaa
     6361 caattccacc aactgcatac gatggctttc aaaaggcaag taccactcac gatcccagtg
     6421 actatgtgag ataatatgta caacaacatt ttccatgaag taaaacctca ttctaaattt
     6481 aaattttaac gttttaaatg taaacttgtg attctgataa gaatcggttg agctaaagcg
     6541 agccccttag cgaatatcca agtaatccaa gaccaactca cagaacatca tgttagccca
     6601 ggagaaccat tcacgagagt agagggtcgg atcatctaca tgaaagcttt cgtgcatgac
     6661 acctgtacca ccatcgcagg caaccagctg atccagcaag aattttttct ctgccttatc
     6721 tcttgttgtc aagccttgga tagaaagggc gattggccag atatagcgat agaaggtatg
     6781 agaactgccg agaccgcttg cgtattctcc ttggtagaag tatggatttt cagagctcaa
     6841 aatggtacga cgagtagctt gatacacttc atcatcgacc gaacagtagc ccagataggg
     6901 cgcagctagt agacttggta catttggatc atccatgatg ctggcatttc ctaggccatc
     6961 cacttcaaaa gcgtaaatct tttcgccctt gctgttggtg gtgtaagcgt agtttttgat
     7021 tccttcttgg atttcatcct gaagacgctt ggcatcagca ataacactct ggctatcagc
     7081 taggtttaat gctgcgaaga tttcttgcac ataacccaag actactacag caaacatatt
     7141 tgacggaatc aagtaactat actggcaaca gtcatcactc ggacgaaaag ctgaccaagt
     7201 catacctgtc actgcaaagt caggtccaaa gccatcattt accaaggtgt cttccttacg
     7261 gtccgtatct cggacaaaac gataaggaga gttcttgtgg tcttgttcca ccgtccacag
     7321 atggagaatt tccttagtcg ctgcgacaaa aatctcatca aactgactag tctcgccagt
     7381 ctctttccag aggagataag ccaactgcaa aggatagcaa agcgaatcca cctcatactt
     7441 gcgctcccag atccagccgt taaggtctgt gtggtcagtc tcgtggtgcc ctttccagtt
     7501 ctcctcaatg ttgaaggagt tagcataggg atccttgagt accaaggtca tctgacgttt
     7561 gaccaaacct gcaatggtct gacgcaggag ggcatctctt ttagctacat gaaggtaggg
     7621 tctgagttgg gctgtcgaat ctcgaagcca catggcagga atatccccag tcaagacaaa
     7681 agttgaacca tcttctaaga tttcaaccgt attgtccaag gtatcggtgt agcaacgctc
     7741 gaaaacatcc acccactctg gatagtcctt agcccgctct gctacttcat ctagccactc
     7801 tctgacaatt tctttcgaat aaaccatttt aaattttacc tctttcaaac aagtttcatg
     7861 ttcagtatat agcttttgag acagaaaata catacacaaa tgatagtcat ttttctacaa
     7921 aatagttatt tttgaaactc cttttctaaa ggccctcttt ttctgatata atgtagaaaa
     7981 acagctaaag gaaagactat gaaaccacta cttgaaacca tcgatacccg ctttggaact
     8041 accaacaagc atgccttttc tcggggaaat accctgccat acacaggcgt gccttttggg
     8101 atgaattact ttgtgcccca gaccagtgac caggatggat cttggttttt tgatccacat
     8161 ctgcctatct ttcaggggat tcgattaact caccagccca gtccttggat tggggactac
     8221 tcttggctcc ttctgacacc tgtcaccagt caactggggg gagacagcct ctttcaccgc
     8281 cagtcttctt atgatataga taaagcttgc ttccagcctc actatttgaa gctcttttct
     8341 ctgcgttatc agattgaaac ccagctcaca ccgacttgct atggtgcttc tattcgtttg
     8401 aatcaaaagc aaggcaaagc cctctccctc tatcttcacg cagcagatga actgacagta
     8461 gagcaagtag ataagcggac tcttgccctg cgacaagaag gtaaaactga aactaacaaa
     8521 aattcgctaa cgatgttcac tgccctgcaa atgaacacgg atattcttgc tatcagccaa
     8581 gaagctggag actggcgaat tgacttagca agtagtcaaa ccgagatgca actagccact
     8641 tctttcatct ctccttctca agctctaatc aatctacctc aagaagattt tgatagctgt
     8701 aaatcaagtg cccaagtgga ttgggaaaat ctcctccatc gttttgacat tatagagaca
     8761 ggagaggctg accgaacctt ctttgaccac tgcctctaca gactcttcct cttcccacag
     8821 actttttatg agattaatga atcagggcaa gccatccaca tggatctggc tactggtact
     8881 gtcaagcctg gtgtcctctt tagcaacaat ggtttctggg ataccttccg caccaccttc
     8941 cccctctttg cccttatcat accggagcac tatcaacgct ttttagaagg tttcctcaat
     9001 agctaccgcg atactggttt ccttccaaaa tggctggctc cagatgaacg gggtatgatg
     9061 ccaggtacac ttttagacgg tattatcgca gatagcgcct gcaaggacat gacccccgac
     9121 ctagaaggag aactcttcca agccatgctc gaaacagcca gcaaagccga ccctctcggc
     9181 atcaatggcc gccacggact agcccaatac caagaactag gttacctctc taccgaccac
     9241 cacgaaagtg ttagccatac tctagactat gcctatagtg acttttgtat cgccagctgt
     9301 gccaaaaaac tagagaacat agaaatcgct gaaacctaca aggctgcttc acaaaattac
     9361 cgccagctat ttgacgctga gacaggttac atgcgagcac gagacaatca aggaaacttt
     9421 caccctgact tctctcctta tagttggggg cgagactacg ctgaatgctc tgccattcaa
     9481 gctactttag gcgttctcca cgacatccct ggcttaatcc aactgatggg tggaaaagaa
     9541 acctttagca actatctttt gaaagcctgt caagatgctc ccctctttga aacaacaggc
     9601 tatggttacg aaattcacga aatgagcgag atggctactg ctccttttgg acaaatagcc
     9661 atttccaacc agcctagttt ccacattcct tatctcttcc gttacagcga ttaccctgac
     9721 tacactgccc ttcttatcaa gacgctccgt cagaaagctt ttcacccaag ttgggaagcc
     9781 tatcctggag atgaagacaa tggtagtctc tctgcttggt acatctggtc agctctcgga
     9841 ttttatccaa cctgtccagg aaaaccaagc tatgacctcg gaatccctct ctttgaccat
     9901 ctacgagtct acctcgctaa agaagataaa tggctggata tccatactaa acaaaaccac
     9961 aaccatttta actttgtcaa agaatgccga ctggacaaaa cactcgtatc aactattcaa
    10021 caccaagacc tcttaaaagc tgaacaacta actttcaccc tcagctggtt accaagtcac
    10081 taataaccaa aagaaccttt gcatcgcgca aaggttcttc ttttatgaaa cttggacttg
    10141 aagttgatcc acaagctgac catccaccgt taggttcaga tagaaatcca aggttttatc
    10201 tcctgcgaaa tacaaggttg gtagcgtcag acttttttca gtctcaccag tttgaaacgc
    10261 aaggacttga atctcatcct gataggcgac accatggaca cctgtccccg gttgaatcga
    10321 aatcttagct tctaaaggac cactacattc tctgcgctta actgtaaaat aggcattttc
    10381 tccctttgcc acagctaggc ttttttctgc aaatgctagt tcctgaacaa cttctttttt
    10441 tgataatcca ggagttttat aaagggaaat cttggtcaac aaaggcaaag cctgtgattc
    10501 tgtaatgact acacgtatct tctgtgcctc aacaactgct cctcgtaaga gacgtttgta
    10561 accaacagta tgacccgaac caaactcctg ccagacacca tccacctcta cttgcacatg
    10621 aaaagcagcg attcgttgcc ctagcttcaa atcttctctt aactcaatta catcaaaagt
    10681 tttaggagaa cctaagtcga gttctaactg gatgggcaag tctgcatcgc ttgcccaaga
    10741 gctggtctca aggccgtctg tcaaatggcg acaagcaaag tctgcggaaa gagctggacc
    10801 agatacctca gctcccagag ccaaatcttc tttatagagc tcattgcgat aggtcgcaaa
    10861 ttcataaagt cgttcaatat cctttgcatc aaagagccca gcttgattcg gcggaatatt
    10921 aagcaagagt ggagttcctc gccctactga gtgaaagtag atttcgacca actcctcgag
    10981 agacttagga tcctgatcct catggtagaa ccagcctgga cggatggaaa catctgcctc
    11041 tccgattgaa aaaatcgtgc ccgagggatc cccgtgctga agatagttca gctctgcttc
    11101 tgttcctagt ttatcaggat tcaccttttg ccacagtgga tcacctgcat accctcgttc
    11161 attgccaatc cagcggatac tggtgccttc tgttgaaaaa atcaagcaat cgccctgcag
    11221 gtcacgaatg gtttcaaacc atttttcaaa ttcataatta accttttgcg cgccctctcc
    11281 tctggcacca tccatccaaa cctcagcgaa cttaccagca ttcccatagt taggatttga
    11341 taagatttcc ttcaactgag ccagataata ggcattgtag tccgcttctc ggtccacatg
    11401 atagagggga ctatgggcat cccacggtga caggtagacc cccatatcca tatcaaactc
    11461 tgtggcagct tgggatactt caaggagcaa gtcgcccttt cctctcctcc aaggactgac
    11521 cttaaccgaa taatctgtgt gagctgtcgg ataaaggaca aagccatcgt ggtgcttgac
    11581 caccaaaatc aactttttga agcccgtttc cttgagcaca cgaacccact cacgcgcatc
    11641 caactgactc gggttaaagc gctcaggatc ctcctgtcca gtcccccatt cttggtcata
    11701 aaaggtatta ggaccgaagt ggataaaagc tgctagttca tctcccagat aagctagctg
    11761 agtctgactt ggtaacggtc catgcggttt gattttcttc atcgttatat atctccttct
    11821 ttagcaactt ctcttattca caaagttatc cacaggttgt ggataataaa ccatgatcta
    11881 acagtcagct ctttccaact ccaactggta ggctcaggat ttttctcctc aatattccct
    11941 cttcaaacag ctctgtcata tggttctgct aacacctctc agaatgctac tctccataat
    12001 tattctcagg cttctttcag gtcattgtgt gaagaccatt cactatccag acttttccac
    12061 agattgtgaa taagtgatta ttctaaacta tcacctcgtc gactataaca aaaaataaaa
    12121 agagggtcaa taagataatt atctcttcct aaatactcag aaaagattct ataatttgta
    12181 caattgaaca agaaatgctt ctccaaaatc tatcttcaaa agaatgttgc atctttttat
    12241 cctttcacac aaacatttct ctttatacag atccaagtga taaccagatg ataattggac
    12301 actttttgat catttgtata ttttttgaaa aattttttaa aaaaatttta tatccttttt
    12361 atagaagttt atgcatttgt atctatgtat tctatcctat gttatgtata ataacataaa
    12421 attgtgctaa acatcataat ttatattatt ttccgagaaa ttatagccat tgacattacc
    12481 taggaattgc gttataacat acatgtaagc ggtacccaaa atggtgccaa gtcaaaattt
    12541 ttaaggagga aaatacatgt cttcacatcc aattcaggtc ttctcagaaa ttgggaaact
    12601 gaaaaaagtt atgttgcacc gtccaggcaa ggagttagaa aacttgttgc cggactatct
    12661 tgaaaggctt ctttttgatg atattccttt cttggaagat gctcaaaaag aacatgatgc
    12721 atttgcccaa gctcttcgcg atgaaggaat tgaggttctc tacctagaac aactcgctgc
    12781 tgaatcattg acctctccag aaatccgcga tcaatttatc gaggaatact tagacgaagc
    12841 caacatccgt gatcgtcaaa ccaaggttgc tattcgtgaa ttgcttcacg gcatcaagga
    12901 caaccaagaa ttggttgaaa aaacaatggc tgggattcaa aaagttgaat tgccagaaat
    12961 tcctgacgaa gctaaagatc taactgactt agttgaatca gagtatccat ttgcaattga
    13021 cccgatgcca aacctctatt tcactcgcga cccatttgca acaattggaa acgccgtatc
    13081 gcttaaccac atgtttgcag acactcgtaa ccgtgaaaca ctctacggta agtatatctt
    13141 caaataccac ccaatctatg gcggaaaagt ggatttggtc tacaaccgtg aagaagatac
    13201 gcgtatcgaa ggtggagacg agttagttct ttctaaagac gtccttgcag taggtatctc
    13261 tcaacgtaca gacgcagctt ctatcgaaaa acttttggtc aacatcttca agaaaaatgt
    13321 tggcttcaag aaagttttgg cctttgaatt tgctaacaac cgtaaattca tgcacttgga
    13381 tactgtcttc actatggtag actatgacaa gttcactatt cacccagaaa tcgaaggcga
    13441 ccttcacgtt tactcagtta cttacgaaaa cgaaaaactt aaaatcgttg aagagaaagg
    13501 tgacttagct gaacttcttg ctcaaaacct tggtgtagaa aaagttcatt tgattcgttg
    13561 cggtggtggc aatatcgtag cagctgcgcg tgaacaatgg aacgacggtt ctaacacttt
    13621 gaccatcgca cctggtgtgg tagttgttta tgaccgcaat accgtgacca ataagatttt
    13681 ggaagaatac gggcttcgct tgattaagat tcgcggaagt gaattggttc ggggccgtgg
    13741 tggacctcgt tgtatgtcta tgccatttga acgtgaagaa gtgtaatcgc tgttcgatat
    13801 tcgtcaatag aaaatgtaaa aaatagaaag aggaaataat aaaatgacaa attcagtatt
    13861 ccaaggacgc agcttcttag cagaaaaaga ctttacccgt gcagagttag aataccttat
    13921 tggtctttca gctcacttga aagatttgaa aaaacgcaat attcaacacc actaccttgc
    13981 tggcaagaat atcgctctcc tatttgaaaa aacatctact cgtactcgtg cagcctttac
    14041 aactgcggct atcgaccttg gtgctcaccc agaatacctc ggagcaaatg atattcagtt
    14101 gggtaaaaaa gaatctactg aagatactgc taaagtattg ggacgtatgt ttgacgggat
    14161 tgaattccgc ggattcagcc aacgtatggt tgaagaattg gcagaattct caggcgttcc
    14221 agtatggaac ggtctaactg acgaatggca cccaactcaa atgctcgctg actacttgac
    14281 tgttcaagaa aacttcggtc gcttggaagg cttgacattg gtatactgtg gtgatggacg
    14341 taacaacgtt gccaacagct tgctcgtaac aggtgctatc cttggtgtca atgttcacat
    14401 cttctcacca aaagaactct tcccagaaaa agaaatcgtt gaattggcag aaggatttgc
    14461 taaagaaagt ggcgcacatg ttctcatcac tgaagatgct gatgaagcag ttaaagatgc
    14521 agacgttctt tacacagacg tttgggtatc aatgggtgaa gaagacaaat tcgcagaacg
    14581 tgtagctctt cttaaacctt accaagtcaa tatggactta gttaaaaaag caggcaatga
    14641 aaacttgatc ttcctacact gcttgccagc attccacgat actcacactg tttatggtaa
    14701 agacgttgct gaaaaatttg gtgtagaaga aatggaagta acagacgaag tcttccgcag
    14761 caagtacgct cgccacttcg atcaagcaga aaaccgtatg cacactatca aagctgttat
    14821 ggctgctaca cttggtaacc tttatattcc taaagtataa ttttagataa taaaccgtct
    14881 accaacagct atgagggctg cgactaatag ctttagtccg gtcctctttt atgtaatggt
    14941 aatctattat ttcttataaa atatgtgaaa aatcattaaa ttgaaatcta aacgcattct
    15001 attgagtgtg ataaaggaga atttatggca aatcgtaaaa ttgtagtagc tttgggagga
    15061 aatgcgattc tttcttctga cccatcagca aaggctcaac aagaagcttt agttgaaaca
    15121 gctaagcatc ttgtaaaatt gattaaaaat ggagatgatc tgattatcac tcacggtaat
    15181 ggacctcaag ttgggaatct cttgctccaa catttggcat cagactctga aaagaaccct
    15241 gccttcccac tcgactcact tgtcgctatg acagaaggta gcatcggttt ctggttgaaa
    15301 aatgctttgc aaaatgctct cttggatgaa ggcatcgaaa aaaatgttgc ctctgttgta
    15361 acgcaagttg tcgtagataa aaatgatcca gcttttgtta acttgagtaa accaatcggt
    15421 cctttctatt cagaagaaga agcaaaagca gaagccgaaa aaagcggagc gactttcaag
    15481 gaagatgctg gccgtggctg gcgtaaggtc gttgcctcac caaaacctgt tgacatcaaa
    15541 gaaattgaaa ccatccgtac tcttttaaat aatggtcaag tcgtcgtagc tgcaggtggt
    15601 ggcggtattc ccgtcgtcaa agaaaacaat ggacatttga ctggtgtcga agcggttatt
    15661 gataaagact tcgcttccca acgtttggca gaattggttg atgcagacct cttcatcgtt
    15721 ttgacaggtg tagattatgt atttgttaac tacaacaagc caaaccagga aaaattggaa
    15781 catgtgaatg ttgcccagct ggaagaatat atcaaacaag atcagtttgc accaggtagc
    15841 atgcttccaa aagtagaagc agctatcgct tttgtcaatg gtcgtccaga aggaaaagca
    15901 gttattactt cccttgaaaa tctaggcgcc ttgattgaat ctgaaagcgg aacaattatt
    15961 gaaaaaggat aagttgtttt actaataaga tgtattc
//
LOCUS       DAVKNL010000045        14875 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVKNL010000045 DAVKNL010000000
VERSION     DAVKNL010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14875
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.045"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(63..1685)
                     /gene="groL"
                     /locus_tag="VW411_001494"
     CDS             complement(63..1685)
                     /gene="groL"
                     /locus_tag="VW411_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEU9794208.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(1701..1985)
                     /gene="groES"
                     /locus_tag="VW411_001495"
     CDS             complement(1701..1985)
                     /gene="groES"
                     /locus_tag="VW411_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEU9794209.1"
                     /translation="MLKPLGDRVVLKIEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(2141..2536)
                     /locus_tag="VW411_001496"
     CDS             complement(2141..2536)
                     /locus_tag="VW411_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU9794210.1"
                     /translation="MYNKVILIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(2614..3375)
                     /locus_tag="VW411_001497"
     CDS             complement(2614..3375)
                     /locus_tag="VW411_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU9794211.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEALKA
                     EFAETFPNQTVWTFLLDVTDMTMVKTVCSDILETIGQIDILVNNAGLALGLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMVKANQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMVHKK"
     gene            complement(3417..4043)
                     /locus_tag="VW411_001498"
     CDS             complement(3417..4043)
                     /locus_tag="VW411_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEU9794212.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLDVERKGKVARVFLKDNGE
                     TVAWNIFEVSSLFEIAERGQVFLTDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKTVQIVAGAPNARVGLKTIVALPGAMMPKGNLIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            complement(4059..4376)
                     /locus_tag="VW411_001499"
     CDS             complement(4059..4376)
                     /locus_tag="VW411_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEU9794213.1"
                     /translation="MIQPASLEELASLVEKAGKKVFIFVADWCSDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKQQINDFLA
                     GLK"
     gene            complement(4373..4672)
                     /locus_tag="VW411_001500"
     CDS             complement(4373..4672)
                     /locus_tag="VW411_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEU9794214.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(5009..5200)
                     /locus_tag="VW411_001501"
     CDS             complement(5009..5200)
                     /locus_tag="VW411_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HEU9794215.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            complement(5361..6035)
                     /locus_tag="VW411_001502"
     CDS             complement(5361..6035)
                     /locus_tag="VW411_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794216.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     EIVYVK"
     gene            complement(6041..6487)
                     /locus_tag="VW411_001503"
     CDS             complement(6041..6487)
                     /locus_tag="VW411_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9794217.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(6601..7104)
                     /locus_tag="VW411_001504"
     CDS             complement(6601..7104)
                     /locus_tag="VW411_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU9794218.1"
                     /translation="MKNYQEWYRNISSRLTSCPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(7101..7463)
                     /locus_tag="VW411_001505"
     CDS             complement(7101..7463)
                     /locus_tag="VW411_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEU9794219.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(7529..9388)
                     /locus_tag="VW411_001506"
                     /pseudo
     CDS             complement(7529..9388)
                     /locus_tag="VW411_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(9427..9876)
                     /locus_tag="VW411_001507"
     CDS             complement(9427..9876)
                     /locus_tag="VW411_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEU9794220.1"
                     /translation="MDKPMLVFKRFGHQIHLMVQKEAKRCGIEFMGGPQGQVVRFLDN
                     REKNQDLVLIKDIEQELNITKPVASNLVKRIVQNGLVELEASPVDKRAKFVRLTDKAR
                     SQMQQVKAFFERIDKQLMEDIDEDELLIFEKVLGQLQAKYQGNRRRE"
     gene            complement(10000..10716)
                     /locus_tag="VW411_001508"
     CDS             complement(10000..10716)
                     /locus_tag="VW411_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9794221.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(11597..13012)
                     /gene="ply"
                     /locus_tag="VW411_001509"
     CDS             complement(11597..13012)
                     /gene="ply"
                     /locus_tag="VW411_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEU9794222.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERNKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(13023..13355)
                     /locus_tag="VW411_001510"
     CDS             complement(13023..13355)
                     /locus_tag="VW411_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794223.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDYLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            complement(13426..14034)
                     /locus_tag="VW411_001511"
     CDS             complement(13426..14034)
                     /locus_tag="VW411_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794224.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(14047..14505)
                     /locus_tag="VW411_001512"
     CDS             complement(14047..14505)
                     /locus_tag="VW411_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEU9794225.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            14630..>14875
                     /locus_tag="VW411_001513"
     CDS             14630..>14875
                     /locus_tag="VW411_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEU9794226.1"
                     /translation="MNYEASKQLTDARFKRLVGVQRTTFEEILAVLKTAYQLKHAKGG
                     RKPKLNLEDLLMATLQYVREYRTYEEIAADFGIHESNL"
BASE COUNT         4316 a         3434 c         2708 g         4417 t
ORIGIN      
        1 aaaaagaagg gttagtcatt ccctcctttt gtgtttttta taagttgttt tctatagaaa
       61 gcttacatca tcccgcccat catgcttgga tccattgctg gagctggggc tactggttct
      121 ggtttattgg ctacgactgc ttctgttgtc aaaatcaagc tggctacaga tgctgcattt
      181 tgtagggctg aacgactcac tttaactgga tcaatgatac cttgatcaat catgttaacc
      241 cactcgccag ttgctgcgtt aaatcctata ccaagctcag catttttcaa acgatcgata
      301 acgatagatc cttcaaatcc tgcattgtga gcaatttgac gaacgggttc ttccaaagca
      361 cggagaacaa tattacgtcc tgttgcttca tctcctgtca attccaaggt agcaacagct
      421 ggaatcacat tggcaagagc tgttccacca cctgcaacaa taccttcttc aacagctgca
      481 cgagtagcgt tgagggcatc ttcaatgcgg agtttcattt ctttcaactc agtttcagtt
      541 gcggctccaa ccttaataac cgctacacca cctgacaatt tggccaagcg ttcttgcaat
      601 ttttcacggt caaattcaga agttgtagtt tcgatttgag acttgataac cgcaacacgg
      661 tgagaaatcg cttcaggatt tcctgcacct tctacaataa ccgtgctatc tttgtccacg
      721 gtcactctcg ctgcttgacc aagagcttca attgtcgcat ctttcaactc aagaccaagg
      781 tcttctgtga taactgttcc gcctgttaag atggcgatat cttcaagcat ggctttgcga
      841 cggtcaccaa aaccaggtgc cttgactgct actacgttga aggttccacg aatcttgttc
      901 aaaacaagag ttggaagagc ctcgccatcc acatcatccg caataatcaa gagtggacga
      961 ttgctttgga gaatgctttc caaaagtggc aagatttctt ggatattgga aattttcttg
     1021 tctgtaatca aaatgtacgg attttcaagg tcagccacca ttttttcgct atctgtcacc
     1081 atgtactgtg aaaggtaacc acggtcaaac tgcattcctt ctacgacttc aagctctgtt
     1141 tccataccac gtgactcttc gatggtgatg acaccgtctt tgccaacttt ttccattgct
     1201 tcagagatgt actcaccaac tttttcagaa cgagaagata cggctgcaac ttgagcgata
     1261 gcttctttat tggcaacagg gatggcgttg tttttcaaag cttcaactgc tgcggcaact
     1321 gctgtttcaa tcccacgacg aataccgatt ggatttgcac ctgctgtgac gtttttgatt
     1381 ccttcacgga cgatagcttg ggtcaagact gttgcagtcg tagttccgtc acctgcgata
     1441 tcattggttt tagaagctac ttctgatact aacttagcac ccatattttc aaaatggtct
     1501 tccaattcga tttctttggc aatggtcaca ccgtcattgg taatcaaggg tgaaccgaat
     1561 gacttttcaa gaacgacatt gcgacctttt ggtcccaagg ttactttaac agtgtctgca
     1621 aggatatcga caccacgaac catggctgaa cgggcatctg atgaaaattt aatttctttt
     1681 gacatactta ctttctcctt ctattcctca atgattgcca aaatgttagc ttcgcctacg
     1741 atgatgtact tttcatcgcc atctttgaca tcaagacctg cgtgggcttc aactaagaca
     1801 cgatctccag ttttaacact tggagcaacc aagtcaccgt tcaaggtacg aacaccttgt
     1861 ccagtagcca caacttgagc tgttttggtt ttttcttggg ctgagcctgc aaggacaaag
     1921 cctccaacag tttgttcttt ttcttctatt tttaagacca cacggtcccc taatggtttc
     1981 aacatctgat ttcctccata atgagataga tattattagc actctttgaa cttgagtgct
     2041 aattcatagt tctattgtat cacttggtca gaaataatca agaaaaaagt ctgactttct
     2101 caagataaaa agcctgagac caactcagac tttttaattc ttaaaatggc aattcttcct
     2161 cttccaagac caaatctgcc aaatcttggc ctgcattatt ttcacgcata gcacgttggg
     2221 cacgactttc caagagttgg aatcctgtga caagtacttc ggtcacgtag ttcatttggc
     2281 catttttctc aaagcgacgg gtacgcaatt ctccatcaac ggaaatgaga ctacctttgg
     2341 ttgcgtagct tgccaaagtt tctgctagtc tgccccatag gaccatattg acaaaatcag
     2401 cttcacgttc accgttttgg tctttgtaac gacggttcac agcgatagtt gctcgcgcta
     2461 ccgacttgtc attgttggtt ttgtgcaatt ctggtgtaga cgttaaacgt ccaatcaaga
     2521 taactttatt atacatattt tcttcctcct acttatctat tcgtaggaaa tcaaaaaaag
     2581 ttacagaaat ttgtaacttt tcgagaaaat tttttatttt ttatgaacca tgaaacctgt
     2641 cgcctgttga ttggccataa tggtcatatc tgtaatctga acacgacgag gttgactagt
     2701 cacatagact actgtatctg caatatcctg agcttgcaaa gcttctattc cttggtaaac
     2761 ggacgcagct cgttctttat caccatgaaa acgcactgta gaaaaatctg tttcgacaat
     2821 tccaggctga atggtcgtca ccttgatatc cgttgcgatg gtatcaattc gcagtccatc
     2881 tgaaaaggtc ttaactgccg ccttggtggc tgagtaaaca gctgcaccag cataggcata
     2941 aattcctgcg gttgacccca tattgataat atgaccttga ttggctttta ccattgctgg
     3001 caagaaacag cgagtgactg ccatcaaacc tttgacattg gtatccaaca tggtcagcat
     3061 atccaactct tcatagtctt gatagggagc taagccaaga gccagtcctg cgttattgac
     3121 caggatgtca atctgaccta tcgtttctaa aatatcagag cagacagtct ttaccattgt
     3181 catatccgtg acatctagga gaaaagtcca aactgtttga tttggaaaag tttctgcaaa
     3241 ctccgcctta agagcttcta gtctgtctat ccgtcgtcct gttagaacga catcctcacc
     3301 ctgctccaga taagcacgcg caatcgcttc accgattcct gatgtcgctc ctgtaatcac
     3361 aacatttttt gccatcttat ttccttctag ctggtctatc agatattaac aacttcttag
     3421 gcagtccagt gtttcgctgg gtcgaacggt gttccgacaa cttggtcttc tgataattca
     3481 agcaccccac gtttttgtgg agcatttggc agatgcaatt cacgaggact gcacatcata
     3541 ccaaaactct tttcaccacg aagttcacct gggaaaatga gattcccttt tggcatcata
     3601 gctccaggaa gcgcgacaat ggttttcaac cccacacgcg cattgggagc tcctgcaacg
     3661 atttgtacag tcttatcact tgcgactgca acttggcaga tgttgaggtg gtcactatct
     3721 ggatgggcta ccatctcaac aatttcacct acaacaaact taggttcctt atcattaaca
     3781 atttcttctg taaaaccttc cgcctgcaac tcttggttca aacgagcgac ttgctcatct
     3841 gtcaaaaaga cttgaccgcg ctctgcaatt tcaaataaac ttgaaacttc gaaaatattc
     3901 caagccactg tttccccatt atctttgaga aaaacacggg ctaccttgcc tttgcgctcc
     3961 acatccagtt tggcatctcc gctatttttc acgatgacca taaggacatc accgacatgt
     4021 tctttattat atgtaaaaat cattgtttcc tttttctcct atttcagtcc tgctaaaaag
     4081 tcattgattt gttgcttgct tttacggtcg cgattgacaa aacgaccgat ttccttgtcc
     4141 ttttctagaa caacaaggct aggaattccg taaacatccc agagtttggc caaatccata
     4201 tactgatctc ggtccattcg aataaaggtg aactctggat tggtctcctc aatctctggt
     4261 aaggcaggat aaatataacg acaatcgcta caccagtctg ccacaaaaat gaagaccttc
     4321 ttgcccgctt tttccactaa agatgctaat tcttctaaac ttgctggctg tatcataaga
     4381 cttcctcctc atagactagg tcttcatttt catagacaaa ggtataatga cggccatcct
     4441 caaaaatgac gccaccaacc aagctctcca gactgctttc gtaaacttga acataaaggg
     4501 tcgcaatttc ccccatgtcg gaaaaatggt ctcgcacaat ctctgtcaac tcttcctgag
     4561 tcttcatgag cttacggtca tctgcaactt ttttcgtagc aagagcaagg cttccgatac
     4621 ctagcagagc caagcctgcc atccacattt ttttagcttt cataccattc attttaacac
     4681 aaaaaaggct tcaggacaaa tgaggaagca gcagaaaagc aagtaaaaag cctcttcctt
     4741 taaggaaaag gacttcttat actcaatgaa aatcaaagac caaactagga agctagccgc
     4801 aggctgctca aagcactgct ttgaggttgt agatagaact gacgaagtca gctcaaaaca
     4861 ctgttttgag gttgtggatg aagctgacgt ggtttgaaga gattttcgaa gagtattatt
     4921 cttattgcca ggcacctaag ttgccaacgt agtaactatc aggtgtgtag gtattgcgaa
     4981 gcatcttacc tgatgaagcc agataatact acttgccatt gtctttgacc caatcattcg
     5041 caatcatgga accagaagaa cttacataat accattctcc cttgtcataa acccaagtac
     5101 tgactttcat ggttcctgag caattaaagg caaaaaaact gtccaatatc attcgttttt
     5161 taaaagcatt tgacactaca tcatctacgc tgtagtacat agatacatca aacgtaaaag
     5221 ccaaaatatc gaaaaatata caacttttaa aggcagcatt tcatttttta aagtctaaca
     5281 accatagaac tgaaatcaga gcaaggcatc aatttcctac tcttagtacg tctattttga
     5341 tggttgaagt gaagattttt ttatttaacg tagacaattt ccaacttccc aaaagccaca
     5401 tctccacgga taatcaaggt tttgctggtt ggggctacag gagcgtcagc cttagctgca
     5461 ccaaaggagg tttctacttt caaatctaca cgccagtgtt gtggaacata gacggttgca
     5521 ttcccgaagg ccacttcaat atttaaagtt gcaaaatcac ctagcatctc tgcattatca
     5581 tagtagattt tagcatcccc aaaagcgact tccacttggt catctacgag atcttgatct
     5641 tgcttataga aggtcccgct accaaaagcg acttccttat ccgtgacgac tgtttttttc
     5701 ccattgtacc accatttttt ttcattccag aacttacttg aatgcgtcag ataaccaaca
     5761 ccaagtacca ccaagatgct agcccaaata agagaatgat tggtaactgg taacaagtca
     5821 taagcgtaat ttgcaatgat gagcgccagt aaacctgtaa aaactgccga agtgagatga
     5881 cgtctaagga tggactcaat ggacttataa gcaaaaaaaa caatacctag taaaggccat
     5941 attttaccat ccaaagaagg aattccaaaa ttcccttgca gcaagatcca agctgctaaa
     6001 accaataaaa caataccaaa tgctttcttt ttcatgtttt ttacctcatg tttcttagat
     6061 tttcttttag gagggattgg taatgccgtg agacatgaac ctccttgtgc gtctgataaa
     6121 aggaaatggt gcccgttcct gaaaaggact tgtccactga gtaaatctga cggatgtttg
     6181 cgatcgttga cttggaaact cgattaaaat agcgaggcaa gatagactcc aactcataga
     6241 gcttgaggcg aacctcataa gcttccttct ggttatgagc gtagatcttg ctcccttctg
     6301 tttcaaagaa gagaatttct gacaggtcta gataatattc acctgtcccc ttgtaaaaag
     6361 tcaacctcgg agcctttgac tcttgcaaga ggcgttgcaa gtctgcaatt tcatctgtca
     6421 aagtgggggt cttgatgaca atttcagtct cctctaaatt gccgtcaatc tcaattcgta
     6481 acttcatcac atctcctttc tgactctact atatcaaagc taagttagga atacaagggc
     6541 aaaaaagcaa gtggtcaatt tgaagcagta agtggttgta ggactagctt aacttacatg
     6601 tcagaatagg aagaaaagga atccccacac caaaccacaa gcatagccaa ccaagacatc
     6661 cttgggataa tgaacacccc ctaaaaccct caccaacccc agcaaagctg aaaagatcaa
     6721 gcatactaag ccaataggta gactagcatg cagacaggcc atggagatga tagttgccga
     6781 aaagacatgg cgactgggca tcgaccgtcc cgaactatcc ttatcaagaa ggggacagat
     6841 atcccaagtt tcataaggtc tcgggtgatt gattttctta cgaaacaggg acaaggccac
     6901 aaaacctgat gcaggaataa gcacataaac cccaacttgc tgaccaagtc ctagttgcag
     6961 gtaagtggtg actagcaaga tcagatagac caggggcatg gcgactgtca tcaaacgatt
     7021 gaaactgcgt aataggaata aaagaattgg acaactagtt agtctggagc tgatatttcg
     7081 ataccattct tgataatttt tcatttatgc ttctcatact tttctagaac ttgttgagat
     7141 gttttcccct tggcaagcca atccactagc ttgtccaagc gacgtatttc ctgcatcaga
     7201 ggatcatcaa tttcctcaat acggacaccg caaatcttcc ctgtaattgc aattcgttca
     7261 gggacataat atggtgcttg tctaaaaaaa tctccgtaag taacatccct atccagacaa
     7321 gcaagaacat cactcacttc ataccctgtc aaccaactgg tcacttggtg aactgactct
     7381 gcctttccac cctttcgctc aacttttgca atcaaggcta agtagacagc agcaaatttc
     7441 atattgtata atttctgact catacttctc tctttctatg tgtactagta taaataagaa
     7501 aaagaaggcc gtcaagcctt cttttgattt attcttctgc ttcatcttct gtaaattgac
     7561 tattgtacaa gtcagcgtag aagccacctt gcgccatcag ttcctcatag ttgccttgct
     7621 cgatgatatt tccatctttc atgaccaaga tcaagtctgc atttcggatg gttgacaagc
     7681 ggtgggcaat gacaaaggat gtgcgtcctt ccatcaaacg gtccatggct ttttggatca
     7741 attcctctgt ccgtgtgtca acagaagaag tcgcctcatc caaaatcaaa agcggtgcat
     7801 ccttaagaag ggcacgagca atagtcaata gttgtttttg tcttacagac aaggtcacgg
     7861 tgtcatccaa gatggtatca tagccatctg gcaaggtcat aataaagtgg tgaattccca
     7921 cagccttact agcttccatc attcgttcat cactaatccc tatttgatta tagatgagat
     7981 tgtctcgaat agttccttca aagagccagg tatcctgcaa gaccattgaa aaggcatcat
     8041 gcacttctga acgcgtcata gccttggtat ccacaccatc aatgcgaata cttcccttat
     8101 caatctcata gaatttcatc aaaagattga caatggttgt cttaccagcc ccagtcggcc
     8161 caacaatggc aaccttttga ccagcatgag ctgtcgcaga gaagtcatag tcttgaacat
     8221 tgacaccgtc caccagaatt tctcctgctg acacgtcgta gaaacgtgga atcagattga
     8281 ccagagttga tttaccagaa cctgttgacc caataaaggc cactgtttga ccagtttctg
     8341 ctttaaagct aacatgttca ataactgcct ccgaatttgc cgcatagcgg aaggtcacat
     8401 ccttaaactc gacctgacct ttgaagtttt catcagtcag ctgcacttga acagggtttt
     8461 ggatagaaga atgcaaatct aaaacttgat taatccgctt agcagagacc atagttcggg
     8521 gaagaacgat gaagagtgct cccatgagaa ggaagcccat gacaacctac atggcataag
     8581 acatgaaaac aatcatgtca ctaaagagag gcagacgcgc tatcggagca gcgtcgttaa
     8641 tcacataggc cccaatccag taaatcgcca cactcaaacc acttgaaatc cccatcatga
     8701 taggattcaa aatagccata agacggttga caaacaaatt caaacgggtc aattcatcat
     8761 ttactgctgc aaatttttca ttttgataat cctctgcatt gtaggcacga acgacacgaa
     8821 tacctgttaa actctcacga gtgatactgt tcagtttatc tgtcagcccc tgaatcaagg
     8881 actgttttgg aaaggctagc gtcatcaaaa cggtcgtcat caggacgttg ataatcactg
     8941 ccacaagtac ggcccagagc cagtattctg aatgacctaa aatcttccca atagcccaga
     9001 tagccataat tgaaccacgc gttaccactt gcaagcccat agtaatcaac atttgaactt
     9061 gagtaatgtc attggtagta cgcgtcaaga ggctaggaat tgaaaatttc ttaatctctg
     9121 tctgcgagta atccaaaact cggttaaaaa tatcacttct cagcctacta gtataagaag
     9181 ccgccactcg ggatgcaaaa aatccaactg caactacgga caagaaggca agaaaggaca
     9241 ttcccatcat catgcttgcc gactgccaca actcatctaa attagtttct tgactaccta
     9301 gcaaatccgt aattttcgag atataggtcg gcacttccaa ctctagatag accgaaaagc
     9361 aagtaaagag aatggctagt aaaatcatcc cccattcttt tctactaatt cttttggcta
     9421 atttctttat tctctcctcc tattcccttg atattttgcc tgtagttgac cgagaacctt
     9481 ctcaaaaatc agtaattcat cttcatcaat gtcttccatc aactgcttgt ctatgcgttc
     9541 aaaaaaagcc ttaacctgtt gcatctgaga acgtgctttg tccgtcagac gaacaaactt
     9601 agcccgctta tcaacaggac tcgcctccaa ttccaccaaa ccattttgca ctatacgctt
     9661 aaccagatta ctagcaacag gcttggtaat attgagttcc tgctcgatat ctttaatcaa
     9721 gaccaagtct tggtttttct cgcgattatc caaaaaacgc acaacctgac cttgcggccc
     9781 acccataaat tcaatgccgc aacgtttggc ttccttttgc accatcaggt gaatttgatg
     9841 accaaaacgc ttaaagacta acatcggttt atccataatc tcccccttct aaataaaaat
     9901 agttctctgg agaataatta aatttctatg agaactattt tcttgattaa aaaaatccca
     9961 agtgattttc tcacttagga tcatgttcta taggttaaat taaaacccat ctacgttcgt
    10021 ataaatcttt tggacgtctt cgtcgtcttc aagaacgctg taaagttttt caaaggtttc
    10081 aaggtcttcg cctgacaatt ccacttctga ctgaggaatc atttccaatt cagtcacttg
    10141 gaattcttca ataccagact cacggagggc aacgatagcc ttgtgaaggt cagttggcgc
    10201 tgtgtaaact gtgattgtac cttcttgtgc ttctacgtca tccacatcca catccgcttc
    10261 gagcaattgc tcaaagactg cgtccgcatc ttcacctcca aatacaataa cacctttgtt
    10321 gtcaaagagg taagaaacag aacctgaagc gcccatgttt ccgccgtttt taccaaaggc
    10381 tgcacggaca ttggctgctg tacggttgac gttagaagtc aaagtatcca caattagcat
    10441 agagccattt ggcccaaaac cttcgtaacg tccttctgta aaggtttcgt ctgtgtttcc
    10501 tttggcttta tcaatcgctt tatcgataat gtgttttggc acttgggctt gtttagcacg
    10561 gtcgataacg aatttcaaag ctgagtttga ttctggatct ggatcacctt ttttagctgc
    10621 tacatagatt tctacaccaa attttgcata tactttagag ttagctccat ctttagccgt
    10681 tttcttggct acgatattgg cccatttacg tcccattagg aatctccttt tttcacattt
    10741 taatctttct tattataaca caagtttttt tgattttcac tagaggaaat ggattttatt
    10801 agcaaatcaa gctaggatag cactttacct gctaagatgg tcttgccttt ctatctttat
    10861 caacaggcac tcatccacat tcaaaaaaca aactagacca ttatctgcaa atagaaagtt
    10921 tcagccaagt ttgacaaagt cagctcaaat tactgtttga agtttgtaga tataagcgac
    10981 aaaaacaatc atactgcacc ttttgttgac agtctactcc agacatatca tagttcaagt
    11041 aaatactttg aaattcaaca gttcttatag gcgctattgt attctaagaa atcaatagaa
    11101 gagtttctaa gcaaacctct aatactcaat aaaaatcaaa gagcaaacta gaaagctagc
    11161 ctcaggttgc tcaaaacact gttttgaggt tgcggatggg gctgacatgg tttgaagaga
    11221 ttttcgaaga gtataattta cgtgttccca agatggagaa gttagactag tacactggca
    11281 cttctaaaac attgctagca attgatttgt tcatatttaa tttcattttt tccataaatg
    11341 ggtattagat ataaacagca aaatatttcc gatacgtgtc gttcttgaat ttccaatcat
    11401 ctaaaacaag taaaggataa tcaatcccct gtatatcaag gaattggcta ccctttttac
    11461 ttttttacac attctgtttg atagattcat tttaacatca cgagcatact ccaatggaaa
    11521 tcgctaggca agagataaac tttcagatat ccgcagagag atcatcgcct ctttttgtcg
    11581 caagcattct cctctcctag tcattttcta ccttatcttc tacctgagga tagagagttg
    11641 ttccccaaat agaaatcgtc cgcttacgca ctagtggcaa atcggttttt tcataaaccg
    11701 tacgccacca ttcccaggca agcccggtac actctctaat tttgacagag agattacgaa
    11761 cattcccttt taaaggaata ctagtggtaa agtgagccgt taaatcctgc ccatttctgt
    11821 cccaagcctt aggagtcaag acttccttac cttgatgatc ataggataat tcattccaag
    11881 taatataata ttgggcaaca taggcaccac tatgatccag cagtaaatct ccgtttctgt
    11941 aagctgtaac cttagtctca acatagtctg tactattttg aaaggtcgca actacattgt
    12001 cacgtaaaaa agaagttgta taggaaatcg gcaagcctgg atgatctgct gtaaagcgac
    12061 tgccttcttg aatcaagtcc tctaccatat ccaccttgcc tgttacaact cgggcacccg
    12121 aacttgggtc gccccctaaa ataaccgcct tcacttctgt attgtccaaa atctgtttcc
    12181 actctgtctg aggagctacc ttgactcctt ttatcaaagc ttcaaaagca gcctctactt
    12241 catcactctt actcgtggtt tccaacttga gatagacttg gcgcccataa gcaacactcg
    12301 aaatatagac caaaggacgc tctgcagaaa ttcctctctg ttttaaatcc tctaccgtta
    12361 cagtatcttg aaacacatct cctggatttt taacagcatc tacgctgact gtataataaa
    12421 tctgcttaaa attaacaatc tgaatctgct tttcgcctga atggacagag ttaaaatcaa
    12481 tatcaagaga attccctgtc ttttcaaagt cagaaccaaa cttgaccttg agttgttcca
    12541 tgctgtgagc cgtgattttt tcatactgca ttctagctgg gacattattg acctgaccat
    12601 aatcttgatg ccacttagcc aacaaatcgt ttaccgctcc gcgaacactt gaattgctgg
    12661 ggtcttccac ttggagaaag ctatcgctac ttgccaaacc aggcaaatca atactataag
    12721 tcatcggagc acgatcgacc gcaagaagag tgggattatt ctctaacaag gtctcatcca
    12781 ctacgagaag tgctccagga tagaggcgac tgtcgttggt agctgttaca gaaatatcac
    12841 ttgtatttgt cgacaagctc cgcttgtttc tttcgataac aacaaactca tcgggtagct
    12901 gattaccctc tttgatgaaa cgattttcaa tactttctcc ctgatgggtc aagagtttct
    12961 ttttatcgta attcatagct agtataaagt catttactgc tttatttgcc atcttctacc
    13021 tcctaataag ttcctggatt gagttgcata aactcagact tgttcagcga aatcagccgt
    13081 ggttggacta agtaatccaa aatttcctcg tacaattctt ctgagacatt gcgtcgccgt
    13141 ctggctaaat aagaagtcgg aatgaccgta ttatccaaca taaatacctt atctaagtca
    13201 atcaaggttg gtcttgtaaa aggattacga gctagatccg gctcttctat cataaagttc
    13261 ttgaccaaac gtctggtcaa gagagctggt ttgaaggtct gatttttaac caactctttg
    13321 tttttagtca tgctgttgtc aatacagata tacatatgat tcttcacagc caaatcgcta
    13381 ctaatagtcg gaaaaggcaa ataaagagct acaacatctc ctctcttaat caagcaagag
    13441 cacccccttt tctcctaatg taacatagac aggattgacc aagtcttctg attgactcag
    13501 aatttccaaa gtttgagttt ggcgcgctgt caatttagta gcatcttgtc tcttcaatac
    13561 aaaatgcttg tcgccaataa ccttgacaat ataatccttc tccaaagctg actggtaaat
    13621 ccacatcaga tgttgtctgt cctgagaact caagagagaa ggattttcaa gcctcccgat
    13681 agtctgataa aaatcaaaaa caggagctaa ctcctgccaa tctgattggc tagttgtcaa
    13741 ggctagaaaa agggctttgc gagctgatac ttcttggtta gccttgagag ttactttccc
    13801 ctccaagttt tttagaaatc gggaaactcc agaaagcaaa tttttctcta actgcgagaa
    13861 ataaaaacct ttcgttccca gacataagtc tttcatgtcg ctttctctag caaataagag
    13921 ctcaaacatt tgatagtaaa agaaaaatat ctggcactgg gtcgcgctca tcttttcctt
    13981 atcggcttct ttttttaacc agagcaaggg cgacaggtag ctggattgag acatttcctc
    14041 tacctcctac tcttttttaa ctggagcatc tgcactagct gccacttctt ttgactggat
    14101 actttcccac tggttaatct cctctgagat aagaccttcg catgtcttga caaatagggc
    14161 aaaagccttg gtctttcctg catatttctc cgtttggcat tgatagagga atttttcttt
    14221 ctccaggagt tgcgcagttt tttggtaaga aatccaattt tcctttgcat tatacaaatt
    14281 gataatcccc tcacacagca agccgagact ggataaggca accgaaatca aacggtagcg
    14341 atcacctggc ataggaatag cacaaaagac agctatgagg aaacctgcca cgatttctgt
    14401 tatttttaat accttatagc gcctacgatg ttgaacgctt ttctttaaaa aatgagctat
    14461 ctgtacgtct aatcgctctg tcaggtacat ttcttctggc gtcatattcg taactccttt
    14521 catttacttt gataatcata gtataacata tttgaatatc aaagtatagt aaaaactgga
    14581 ctataactta ttagacttcc tgcgaaacaa aatatggtat agtagttcta tgaattatga
    14641 agcaagtaaa caactaactg atgcacgatt taagcgtctt gttggtgttc agcgcacgac
    14701 ttttgaagag atattagctg tattaaaaac agcttatcaa cttaaacacg caaaaggtgg
    14761 acgaaaacct aaattaaacc tagaagacct tcttatggcc actcttcaat atgtgcgaga
    14821 ataccgcact tatgaagaaa ttgcggctga ttttggtatt cacgaaagca actta
//
LOCUS       DAVKNL010000046        14673 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVKNL010000046 DAVKNL010000000
VERSION     DAVKNL010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14673
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.046"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            <1..422
                     /locus_tag="VW411_001514"
     CDS             <1..422
                     /locus_tag="VW411_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU9794227.1"
                     /translation="SDKRFYRPTFRMHLTNKEILDKILSYSEDLKHHYQIYQLLLFHF
                     QNKDPEKFFGLIEDNLKQVHPLFQTVFKTFLKNKEKIVNALQLHYSNAKLEATNNLIK
                     LIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSQA"
     gene            complement(472..1182)
                     /gene="deoD"
                     /locus_tag="VW411_001515"
     CDS             complement(472..1182)
                     /gene="deoD"
                     /locus_tag="VW411_001515"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU9794228.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHCVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKKLGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(1836..2348)
                     /locus_tag="VW411_001516"
     CDS             complement(1836..2348)
                     /locus_tag="VW411_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEU9794229.1"
                     /translation="MSAQITINHKKARYVRIELEGYNALSLAEVEVFCFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGSGR
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            complement(2373..3353)
                     /locus_tag="VW411_001517"
                     /pseudo
     CDS             complement(2373..3353)
                     /locus_tag="VW411_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855254.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
     gene            complement(3829..4638)
                     /locus_tag="VW411_001518"
     CDS             complement(3829..4638)
                     /locus_tag="VW411_001518"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU9794230.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            complement(4654..5196)
                     /locus_tag="VW411_001519"
     CDS             complement(4654..5196)
                     /locus_tag="VW411_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEU9794231.1"
                     /translation="MTRYALLVRGINVGGKNKVVMAELRQELTNLGLEKVESYINSGN
                     IFFTSIDSKAQLVEKLETFFAVHYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYHKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            complement(5198..6409)
                     /locus_tag="VW411_001520"
     CDS             complement(5198..6409)
                     /locus_tag="VW411_001520"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEU9794232.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIYDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFFPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            complement(6423..7106)
                     /gene="rpiA"
                     /locus_tag="VW411_001521"
     CDS             complement(6423..7106)
                     /gene="rpiA"
                     /locus_tag="VW411_001521"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEU9794233.1"
                     /translation="MENLKKMAGITAAEFIKDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTSKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            complement(7404..8243)
                     /locus_tag="VW411_001522"
     CDS             complement(7404..8243)
                     /locus_tag="VW411_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEU9794234.1"
                     /translation="MKVIDQTLLEKVIIERSCTSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEAMAFSLQDQQLLQEQLEKAEVVLLGPGL
                     RDDTFGENLVKQVFASLKKNQILIVDGGALTILARTSLLFPSNQLILTPHQKEWEKLS
                     GIAIEKQNEGTTSSALTSFPQGTILVEKGPATRIWQVGQSDYYQLKVGGPYQATGGMG
                     DTLAGMIAGFAGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISNCLPKVMK
                     RYV"
     gene            complement(8258..9079)
                     /locus_tag="VW411_001523"
     CDS             complement(8258..9079)
                     /locus_tag="VW411_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATPase"
                     /protein_id="HEU9794235.1"
                     /translation="MILKIYNGEYSLQWDGIYYLALIDYPNIQEWELEKIAKFIAYEK
                     LHKRQTSIECADSCLKKEILDYICQHPFLPPFTPTDKRVASTYDLHKRLVTSDYCSHT
                     TTIDAAISIFKTGRLLSAVKAFGRDAEELVLDSRNAASDPIDYFDYVMLGWSNTSSGY
                     RLAMERLLGRAPSEKELQDKFIPGVSFHFIYTDLIKVPGYIFDGYHAVKIKDMLNLLS
                     ELYICIIPTHNKSQFENIIPTKIQDRVYYLDYAGEDLEEWTKKVYQVVLKQSDKG"
     gene            complement(9217..10074)
                     /locus_tag="VW411_001524"
     CDS             complement(9217..10074)
                     /locus_tag="VW411_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEU9794236.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETGLVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGFRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLSKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            complement(10253..10987)
                     /locus_tag="VW411_001525"
     CDS             complement(10253..10987)
                     /locus_tag="VW411_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9794237.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKVIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGDSKAVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQGQRTKDFLSKVL"
     gene            complement(10987..11670)
                     /locus_tag="VW411_001526"
     CDS             complement(10987..11670)
                     /locus_tag="VW411_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9794238.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            11860..12090
                     /locus_tag="VW411_001527"
     CDS             11860..12090
                     /locus_tag="VW411_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEU9794239.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            12319..14577
                     /locus_tag="VW411_001528"
     CDS             12319..14577
                     /locus_tag="VW411_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9794240.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPSISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
BASE COUNT         4412 a         3290 c         2638 g         4333 t
ORIGIN      
        1 tgagcgataa acgtttttat cgccctactt ttcgcatgca cttaacaaat aaagaaattc
       61 ttgacaagat tttaagctat tcagaagact tgaaacacca ctatcagatc tatcaactct
      121 tactttttca ctttcagaac aaagaccctg agaaattttt cggactcatt gaggacaatc
      181 tgaagcaggt tcatcctctt tttcagactg tctttaaaac ctttctaaag aacaaagaga
      241 aaatcgtcaa cgcccttcaa ctacactatt ctaatgccaa actggaagcg accaataatc
      301 tcatcaaact tatcaagcgc aatgcctttg gttttcggaa ctttgaaaac ttcaaaaaac
      361 ggatttttat cgccctgaat atcaaaaaag aaaggacgaa atttgtcctt tctcaagctt
      421 agcttttttt caacccacta cagttgacaa agagcccctt tttggctata attattctgc
      481 aatcaaggtt tccaaaccaa ccttcatcat atcagtgaag gtattttgac gttcttctgc
      541 agttgtgtct tcgtctggat tgaccaagct atcagagatg gtcatgatag ctagcgcatc
      601 aacatggtat tgggcagcaa gatagtaaag agctgctgct tccatttcca cagccttgac
      661 tccccattta ccaagctcga tattcttttc aaagtaattt gagtaaaaga catcagatga
      721 caaaacgttc ccaacgtgag tagtcatacc aagttttttg gcgatatggt aggctttatc
      781 aagcaaatca aagctagcaa tttgtggaaa atcgtactgt ggccagtcat tacgaacgat
      841 gtttgagttg gttgcagccg cctgcgccaa aactaattca cgaacatgaa cctcttcatt
      901 caaagaacct gcagttccca cacgaatcaa tttcttcaca ccgtagtcta cgattaactc
      961 acgcgcataa atcgaaatag atggcattcc catcccagtt cccatgacag atacacagtg
     1021 acccttgtaa gtaccagtgt aaccaaacat gttacgcact tcgttaaaac aaacagcatc
     1081 atcaaggaaa ttctccgcaa taaacttagc acgaagagga tccccaggaa gaagaatttt
     1141 atcagcaatt tcaccctgct gagcagcaat atggatagac ataatttatg atacaaagag
     1201 cgagaagaaa acgactgaaa attaggaacc tgacgagaaa tcctgatttt tcagtcagat
     1261 tatctatttt ccgagttttc cgctcgtgtt caaatcaaaa cacacgctct acctttcttt
     1321 attttatatt ttatattgag aaagatacca aacccatcaa aaagcgaagg gaaaatagga
     1381 gttgggcgca gtgagcgatg ctcgctagat caactatctt tttcccacta cttttagggt
     1441 ggggtcaatt cctttcttaa ttttgattta gaggagagtc gcccgtattc agttcagcga
     1501 atacagttta cccatccttt cgtttttgtt tttagaaaag ttttctactc gtgttcaaat
     1561 tagaacacgc gctctacctt tctgtttata ctcttcgaaa atctcttcaa accacgtcaa
     1621 cgtcgccttg gattatatat gtgactgact tcgtcagtct tatctacaac ctcaaagcag
     1681 tgctttgagc aacctgcggc tagtttctta gtttgctctt tgattttcat tgagtattaa
     1741 ttttgttctt tcatagagag ctacctgagt cctattcaag ctcaggtagt actttcttat
     1801 aaactagaca aactaactgt cattctacca tcagattaca agacatcatc gtcactcacc
     1861 ttggaattca atgtcgtacc ccaatgggta attttacggt ggggttgagc taaaattggt
     1921 ctgttttcat agattgtttg ccatctattc catagtaggc ccgtcttttt ctcaatctta
     1981 actcgcagat ttctcatatt ttctttgatt gggaggttga ggacaaaacc tgcagtctgg
     2041 ttgcgaccgc ttccttccca agaatgacta cgaacaactt ggtttccatc tttatctact
     2101 ggaacttctt cccaagttat ggagtagcgg gcaatgtaag ctccactgtg ttgaattgtc
     2161 aatgttttat ccttcacagg agtctgactg attggttgaa ctggcttaga aacttgtgtc
     2221 gccgtttcag cattcgtagc tataaagcag aaaacttcaa cttctgcaag actgagggca
     2281 ttatagcctt ctagctcaat ccgaacatag cgcgcttttt tatggttaat cgtaatttgt
     2341 gctgacacat ctttaggaga tgttatacgt tttcactcaa tttctttacc agaactgtct
     2401 aaaagaatga catcaaagtt tgccaatcta tcctgggcag tgtctgttcg gttgtaaata
     2461 ttgatttggc gaatggtttc ttctttagcc aaatctactt gccaccaagg cttagattgg
     2521 aagtttgtat gagtgacaga attgtgacca tagttaccat cgactttgcc atccacagct
     2581 cttctagcat ctcctccgaa agctgtcgaa ctttgagtaa cctgttttcc tgaggcaata
     2641 ttttccattg gttcagcgct tggttgacta gaagatcgag gcagagcctg tttcttaaag
     2701 aatcgcactt tatccaattt cggatcacga taaccatgtt tatccaaggt agcttgttta
     2761 ccaatattaa agttaggaat atcattcatt ggactttcaa aatagccacg agaacggtgt
     2821 ttaaagcgtg aattacctgt aaatgtaaca atatcacgat tattatgaat caagggagtc
     2881 acaacattgt cattatcaac cacataatgc ttaatttttc cactaacagc taatttacga
     2941 ctttccaaac caacatccca gaaaccattc ttagcattcc aaacagttct ggaagtaatc
     3001 actttaggag cactgaaagt tgtcactttc ctcaatacat ggttataaaa atcaggatat
     3061 ttttggtaag cttcaaccgc tgcaatctga gctaggtaac atccaagaga atgacctgtc
     3121 atatacaact tagtaatact tatatccttg gctaattccc caacaacctt gcggatatca
     3181 tccgcttgag ctggttatgt tatctctact tttgactctt cccttttgtt ttcaagaact
     3241 tcacttgctt ccggaagcgt ttgaggagct agtcttggct cctctctaag aggagtttct
     3301 agttttggag catcattttc tgactctttc aattcctcct tagaatctag cacacctatt
     3361 accacttcag atgttacatc ttgctttact tcattggctg aaattccagc tggagctaga
     3421 aatcaaagcc gactacaaca gatacaactc cgatacttaa ttttttatgc caaagcgcat
     3481 aattcgttga ccaattttca ttatttctta tctttctttt cgtttattta caagcagact
     3541 caagcgaaat ctcattgtgc tatatatgat aagactgaga aattcagaaa gtcaattcat
     3601 tctcatctct ccttatcata ctagtctgct tggctaggaa gctaatgcta gtatcctaga
     3661 atcttgtctt tcacaatgct ggctatcaga tcagacatac ctattcttcc gcttctttca
     3721 gttataagag caggaagaag ctcatagtat tcctctgttt cttctttaat ccggataggc
     3781 agtcaatcct gaaaaaacag aggcactccc ccttttaaat cttttttctt acaattcagc
     3841 aagaatcgct ttaagcaagc ctttgaaatc acctttaaca cgttcagtca cttctacaac
     3901 ttcttcgtga ttgagttctt cttggaaacc ggccgcaaag ttagtgatac atgaaattcc
     3961 cagaactttc aagccagagt gggctgccac gataacttca ggaaccgtag acataccaac
     4021 tgcatctgct cccagtgtct tataggaacg aatttctgct ggtgtttcat aagtcggacc
     4081 agtaactccg atatagacac cttcatcaag cttgatatta agttttttag ccacttcatg
     4141 ggcagtggca cggtattctg gtgtgtaggc cctagacata tctgggaaac gtgggccaaa
     4201 gtcatccaag ttttcaccca tcaatggatt ttgccccgtc atgttgatat ggtctgagat
     4261 agccatcaag gtaccaggac caaatccgat accgccagct gcattggtta caataacacc
     4321 ttcacatcca agaactttca tcacacgaac tgggaaagtc accacttcca gaggattccc
     4381 ttcatagaaa tggaaacgcc cttgaagagc caagaccttg cgacctgcca gttcaccata
     4441 taccaattta ccagcatgac cgactactgt tgaacggccc cagtttggaa tctcagcata
     4501 gtctactaca actggatttt cgatttcttc tgccaattct ccaagtcctg atccaaggat
     4561 tagaccgaac tcaggggctg caattccctt ttctttcagg aaagtagcag tttcatggat
     4621 tttgtttaaa aatgtcattg tgtgtctcct ttattatttt tttagcattt gaccaatttt
     4681 gtcaaaggtt ttagcattac gaatagtaat gtggcggtag aaaggcacct tcagcaagta
     4741 cttatgatag gcagtcttag aataggattc ttcagaaaat ttcccccaga aaatcccaag
     4801 ttttccaaaa taaagcactt catctttcag ctctaaactt tcaactgtcg cgatgacttg
     4861 gtccacatcc aaaccctcag tgtaaaagag aaaatctttt cgtgccaagt ctctgctcca
     4921 ccaagctggt agattttcaa gttccgcctc aaagtcctct agactcagta aagaaaagct
     4981 ctgaataaat ggataatgga ctgcaaagaa agtctctagc ttttcaacca attgggcttt
     5041 ggaatctatc gaagtaaaga aaatattgcc actattgatg tagctctcaa ccttttccag
     5101 tcccaagttt gtcaattctt gacgaagctc cgccatgacg accttattct taccaccaac
     5161 attgatacct ctcaccagca aagcatagcg cgtcatctta taccaattta tctaagaaac
     5221 tttccccaat catagcagtt tccacaccaa agttatcggc aacagtcgct gaaatatctg
     5281 caaaatgtcc tactggaatg agaccatttc ctttaaaggc agggctatag gccaacaatg
     5341 gaatatattc ccgagtgtga tccgttcctg catacgttgg gtcatttcca tggtccgcag
     5401 taatcaagag aaggtcattc tctctcatag ctgcgataat ttcaggtaag cgttcatcaa
     5461 actcatgcaa gcaatcacgg taaccgtgag cattacgacg atggccgtaa agggcatcaa
     5521 agtcaactag gtttgtgaat gagaatcctt tttcaaactc agcaagtccc atagtcttca
     5581 atagtgtatc aattccatga ctatttgact tgttgtgacc catgtcatgg ttgataccag
     5641 caccgttaaa gatatcgttg attttaccca cagcataagt atcgatacca gcctcattca
     5701 atttatccaa aacagttggg aaaaatggag atacagccaa gtcacgacgg tttgccgtac
     5761 gagtgaagtt acctggttca cctacataag ggcgagcaat gatgcgacca agaagggcag
     5821 gacgctcaag ggtaatcgaa cgagcgtatt cacagatacg gtacaattca tccaaaggaa
     5881 taatgtcttc gtgggcagca atctgcaaaa cagggtcagc tgaagtatag ataatcaact
     5941 ctccagtttc catctgacgt ggtccaaaat catagataac agccgttcct gaataaggtt
     6001 tgttggcttc acgaataacc ttgcgtcctg agaattcttc gatttttgtc aggatttctt
     6061 ctgggaatcc gttccagaaa gtatcgaaag gctcagtaat gttgagtccc atgatttccc
     6121 agtgtccagt catagtatcc ttaccaagag atacttcctc taattttgtt gcatatccag
     6181 ttggattgct ttcagctgct acagtcttaa gaggagtttc acgaggaata tttccaagac
     6241 ctattttagc catgtttggg acattcaaac caactgtttt tgaaatgtgt cccagtgtgt
     6301 cagaagctcc atctggaacc cctgcattga caaagttatt agcatctggt gctgcaccga
     6361 ttcctacaga atccagtacc accaaatgaa tacgattaaa tttagacatc ttatgccccc
     6421 ttctattttc ctttttttga agttgaaatc tgaactccat ctcgtccagc aacgattacc
     6481 ttatccacca tttggttgaa taaaccatgc tccacaacac caacgacatg gtccaattct
     6541 tgtccaaaag caattggatt ttcaatgaca tccaaggcga ggtcaatgat aaaattctgc
     6601 atatcggtca caaaacgttg gccgtctttt tcacggaaac ttggtttgta gccagctcgt
     6661 tcaaaatgac gaaagacctg ctctgcacca tactgaacca cttctactgg caatttaaaa
     6721 gcacctagtt tttcgaccag cttgctttca tccaccaccc aaatgtattc ttttgatggt
     6781 gttgcgacca ccttttccat gagaagggca ccaccaccgc ctttgattcc attaaactga
     6841 ctatccactt catccgcccc gtcgactgtc acatcgacaa agtctacttg gtcaatagac
     6901 ttgagcggga tattgagccc ttcagcctgt ttactggtca cactagaagt cgtcacagct
     6961 gtaatctgca agccttcttc cttgattcga cgaccgattt cttcgacaaa ataataggca
     7021 gtagaacctg ttcctagccc tacaaccatc ccatccttga taaattcagc agccgtgata
     7081 cctgccattt tcttcagatt ttccactcaa acacctccat taaagagcta cctctattat
     7141 accatgtaaa ggcttttatt tcatctaaaa gtacctttag aatactgctt atttatcctt
     7201 gaaattgaat ttttttaata aaagtagagc tctaacaaat caatttaagt acggatgtaa
     7261 accaaatcaa ctctaacaaa aatagattta caagagtaaa aaaacgaaca aatttagttt
     7321 cttagcaaga agaatactaa acgttcgttt tttgtgaatt aagaataatg atacaaatta
     7381 tcaacatttt ttgtctaact attttagaca tatcttttca ttactttagg aagacaatta
     7441 ctaatttccg tcggcaagac cacataattt tcttgagata gttcttgggc tatggctgaa
     7501 tgaagatggg ttgctactgc cacacgttcg tagagactgg cctgtcgaaa ttggcctgca
     7561 aatcctgcaa tcattccagc cagtgtatca cccataccac cagtcgcctg atagggaccg
     7621 ccaaccttta actggtaata atcagactgg ccaacttgcc aaatacgagt agctggacct
     7681 ttctctacca aaattgttcc ttgagggaaa gaagtcaggg cactagatgt tgtaccttcg
     7741 ttttgctttt caatagcaat accagacagt ttttcccatt ctttttggtg gggagttaag
     7801 ataagctggt tagatggaaa caacaaactt gtcctagcaa ggatggttaa ggcccctcca
     7861 tctacaatca aaatctgatt cttttttaag ctagcaaaga cctgttttac aagattttct
     7921 ccaaacgtat cgtctcgtaa accaggcccc agcaagacaa cttctgcctt ctccaattgc
     7981 tcttgtaaca attgctgatc ttgcagagaa aaggccatag cctcaggcaa atggctgtgt
     8041 agagcaggga tattttccct gtccgttcca acggttacca atcctgcacc gctttttaca
     8101 gctgctaaag cagccatgat gatggcacca ccataaggat aagtcccacc aagcaacagc
     8161 agacgaccgt agtctccttt atgacttgta caagaacgtt caataatgac tttttctagt
     8221 aaggtttgat caatcacttt catcgttttt tcctcaacta tcctttatct gattgtttta
     8281 aaacaacttg atagactttc ttagtccact cttctaagtc ttctccagca tagtcaagat
     8341 aatacaccct atcttgtatt ttggttggaa taatattttc aaattggctc ttattatgag
     8401 ttggaataat gcaaatatac aactcactta ataaattaag catgtcctta atttttacag
     8461 catggtaacc atcaaaaata taaccaggaa ctttaatcaa atctgtatag ataaaatgaa
     8521 aacttactcc aggaataaac ttgtcttgta attctttctc tgaaggagct cgacctaata
     8581 aacgctccat cgccaatcga taaccagaac ttgtatttga ccaccctaac atgacatagt
     8641 caaaataatc tatcggatca gatgcagcat ttcgactatc caaaaccaac tcctcagcat
     8701 ctcgcccaaa ggctttcaca gcagataaaa gacgaccagt tttaaaaata gaaatcgctg
     8761 catctatagt cgtagtatga ctacagtagt ctgaagtcac taacctctta tgtaggtcat
     8821 aagtcgaggc tactctttta tctgtaggag taaatggtgg cagaaaggga tgctgacaga
     8881 tgtaatctaa aatttctttt tttaaacaag aatcagcaca ctcaatactt gtttgacgtt
     8941 tatgaagttt ttcgtaagct ataaatttag caattttttc taattcccac tcttgaatat
     9001 ttggataatc aattagtgct aagtagtata ttccatccca ttgtaaacta tattccccat
     9061 tataaatttt taaaatcatc taatcacctc actccattat acaacaaaaa ggaggcgcaa
     9121 acctcctttt ataaacttat ttctaaaatt taatattcat ttctatcatt ttagatatag
     9181 ctatagaaaa tacactttcc tcagaaaatt tttatcttat tttctatcca atgtcctaag
     9241 tgctgcctga taggtttgct ccatcagcat agtaatggtc ataggaccga cacctccagg
     9301 gactggcgta atgtggctag caagtggggc aaccgcctca taatcaacat ccccacagag
     9361 cttaccattt tcatcgcggt tcatcccaac gtcaatgact accgcacctg gtttgacaaa
     9421 gtcagcagtc acaaacttgg cacgaccgat tgcaacaacc agaatatctg cttttgcagc
     9481 caccttggaa agattatgag tacgtgagtg agtcaaggtt actgttgcat tctttgccaa
     9541 aagaagctgg gccataggtt ttccgacaat attggatcga ccgatgacga ctgcattttt
     9601 accttccaag tcaatcccat attcatggaa catttccata attcctgccg gtgtcgaagg
     9661 aatcatgact ggatgaccag accaaagacg ccccatgttt agaggatgga aaccatccac
     9721 atccttttct gggtcaatag ccaatagaac cgcctcttca tcaatgtgtt ttggtaatgg
     9781 caactgaacc aaaatcccat gccaagctgg atcctgattg tatttagcaa tcaggtctaa
     9841 caattcctct tgagtaatgg tctctggaac ccgtactact tcgctacgga aaccagccgc
     9901 aagggctgac ctctccttgt tgcgaacgta gacttggctg gctggattgt ccccaaccaa
     9961 aatcactacc aaaccaggca ctagacctgt ttcttccttt aattttgcag tcttttcagc
    10021 caactgcccc tgcaatttgg ccgctaaagc tttcccatca ataatctgtg tcatatcact
    10081 tctcttttct tacaaatatc tttaattata ccaaaaaata caatgtagct ctaacacttc
    10141 tttcctaggc aatcctatag ctttaaacta taagaaaaag ctctggttga gcttttcact
    10201 aatcttctct ttaacgttta aagctggcta caaatagcta aacaaagcta acttataaaa
    10261 ccttactcaa gaagtcttta gtcctttgtc cttgggtttg ttcaaaaatc tgctcaggtg
    10321 ttccgtcttc aacaaccaca ccgtctgcca taaagatgac acgatctgcc acctcacggg
    10381 caaatcccat ctcatgtgtt acgataacca tggtcattcc tgacttggct agatcttgca
    10441 taacagccag aacttctcca accatctcag gatctagggc tgaagttggc tcgtcaaaga
    10501 gcaaaacatc tggttccata gccaacccac gcgcgatggc aatccgctgt tgctggccac
    10561 ctgacaaact ctgtggataa gcgtctgcct tatctggcaa accaactttt tccaaaagtt
    10621 cctgagctct tttctctgca acggccttac tgtcaccttt ggtcttgata ggggacaagg
    10681 tgatattttc catcacagtc atattaggaa agagattgaa ttgttgaaaa accatgccca
    10741 tcttctcacg catggcaaac aggtcattct tcttgtccgt aatatcgact ccctcaaaga
    10801 taaccttccc cttggttgct tcttccaaca aattcataga gcgaagcaag gtagatttcc
    10861 cgctccctga aggaccgatg ataacgacaa cttctcctct tttaatctcg aggttgatgc
    10921 ccttcaatac ttcattcttt ccaaaggatt tatgtaaatt ttcaattttt atcaaggttt
    10981 ctgtcattat ttcttatctc cttgtcccat atgtttttca aaagctttca aggctactgt
    11041 cagaatagag gtcataatca agtagtaaaa tgctgcaaat aaaagtggtg ttaaaggtag
    11101 ataggttgtt gtagaaactg ttgtagcccc attccacaac tccatgaccc caatagctga
    11161 taagagggag ctgtccttga taatggtgat aaattcgttc cccaatgctg gcaagatatt
    11221 tttgactgct tgtggcaaaa tcacataacg catcgcattt ttaggacgaa tccctagcga
    11281 ataagccgct tctagctgac cttttggaac cgcattgatt ccggcacgaa cagtctccga
    11341 aacataagca ccactattca tagagataat caaaatccct ggaatcagac gcgaaaaatc
    11401 aacacctaaa attccaatct gaatagtcgg agcattgata tgcataagag caaaggcaat
    11461 cataatttga accatcatcg gtgtcccacg gaaaatccaa acgtacaagt tggccaacca
    11521 aacaagcggt ttaaactttg aacgttgccc aaaagccaag acaacaccca aaatagttcc
    11581 caaaaagata acacagatag aaataagaat cgtcacaaca gccccatagt taaaataagg
    11641 taaatactta ggtaaaaaag aaaaattcat agtcacactg ctttctaaaa tagaatactg
    11701 tatgattata acatgttttg aattaaaatt taaatttttt atccaattta tttaaaaata
    11761 tttaaactag cagaattagc gagaaatctt agttttttct agtcaagttg gcctccttta
    11821 tggtaaaata gtaacgataa gaaatgaagg agaatcaaaa tggaatcaca tttggttaga
    11881 atcatcaacc gccttgaagc aatggcaaac gatggcggaa atttaaaacg taattttgaa
    11941 cgcgaaggag ttgttgttgc agaagttgcg tacagccacg acgaagaaaa cggcccaatc
    12001 tttacccttc gtgacgtaga agatcgcgaa acgtatacgt ttgatagcat tgacttaatc
    12061 gcaatggaaa tttacgaact tctctactaa tctaaaacaa gctgggattg tccctgcatg
    12121 tctaagtaaa atcctttttg tctcacaaat aggatttttt tatagtccaa attcacaaaa
    12181 attttcattg taacaacttc tcaaagcttc aatcttttta cttgctttac accccaatcc
    12241 tgttataatg agagtataga atttgactat ttttgacctt taaacaagtc aggctaaaga
    12301 aagaaatgag gtagatgtat gctttgtcaa aactgtaaaa ttaacgactc aacaattcat
    12361 ctttacacca atctcaatgg aaaacaaaaa caaattgacc tctgtcaaaa ctgctataag
    12421 attatcaaaa cagatcctaa caatagcctc ttcaaaggta tgacggatct gaacaatcgt
    12481 gacttcgatc cctttggtga tttcttcaat gatctaaaca atttcagacc ttctagcaat
    12541 actcctccta ttcccccaac ccaatcaggt ggaggttacg gtggaaacgg cggttatggt
    12601 tcccaaaatc gtggatctgc tcaaactccg ccacctagcc aagaaaaagg cctgctggaa
    12661 gaatttggta ttaatgtaac tgaaattgcc cgtcgtggag acattgaccc cgttattggg
    12721 cgcgacgatg agattatccg tgtcatcgag attctcaatc gtagaaccaa gaataatcct
    12781 gtccttatcg gtgaacctgg tgtcggaaaa acggccgttg tcgaaggtct agctcagaaa
    12841 attgtcgatg gcgatgtgcc acataaactc caaggtaaac aagtcatccg tctggatgtg
    12901 gttagcttag ttcaaggaac ggggattcga ggacaatttg aagaacgcat gcaaaaactc
    12961 atggaagaaa ttcgcaaacg tgaagacatc atcctcttta tcgatgaaat ccatgaaatt
    13021 gttggtgctg gttctgcgag tgatggtaat atggacgcag gaaatatcct caagccagcc
    13081 cttgctcgtg gagaactgca actagtcggt gctactaccc tcaatgaata ccgtatcatt
    13141 gaaaaggatg ctgccctcga gcgtcgtatg cagcctgtta aagtcgatga accaacggtg
    13201 gacgaaacaa tcactattct caaagggatt caaaagaaat acgaagatta ccaccacgtt
    13261 caatatacag atgctgcgat tgaagcagct gcaactcttt ccaatcgcta catccaagat
    13321 cgcttcttgc ctgacaaggc cattgacctc ctagatgaag ctggttctaa gatgaacttg
    13381 accttgaatt ttgtggatcc taaagtaatt gatcagcgct tgattgaggc tgaaaatctc
    13441 aagtctcaag ctacacgaga agaagatttt gagaaggcgg cctacttccg cgaccagatt
    13501 gccaagtata aggaaatgca aaagaaaaag atcacagacc aggatactcc tagcatcagc
    13561 gagaaaacta ttgagcacat tatcgagcag aaaaccaata tccctgttgg tgatttgaaa
    13621 gagaaagaac aatctcaact catccatcta gccgaagatc tcaagtctca tgttattggt
    13681 caagatgatg cagtcgataa gattgccaag gctattcgcc gtaatcgtgt cggacttggt
    13741 acccctaacc gcccaatcgg aagcttcctc ttcgttgggc caactggtgt cggtaagaca
    13801 gaactttcca aacaactggc tatcgaactt tttggttctg ctgatagtat gattcgcttt
    13861 gatatgagtg aatacatgga aaaacatagt gtggctaagt tggtcggcgc tcctccaggt
    13921 tatgttggct atgatgaggc tggtcaatta actgaaaaag ttcgccacaa tccatattct
    13981 ctcatccttc tcgatgaagt ggaaaaagct cacccagatg ttatgcacat gtttcttcaa
    14041 gtcttggacg atggtcgttt gacagacggg caaggacgca ccgttagctt caaggatgcc
    14101 atcattatca tgacctcaaa tgcaggtaca ggaaagaccg aagctagcgt tggatttggt
    14161 gctgctagag aaggacgtac caattctgtc ctcggtgaac tcggtaactt ctttagccca
    14221 gagtttatga accgttttga tggcattatc gaatttaagg ctctcagcaa ggataacctc
    14281 cttcagattg tcgagctcat gctagcagat gttaacaagc gcctctctag caacaacatt
    14341 cgtttggatg taactgataa ggtcaaggaa aagttggttg acctaggtta tgatccaaaa
    14401 atgggagcac gcccacttcg tcggactatt caagactata ttgaggacac aatcactgac
    14461 tactaccttg aaaatccaag cgaaaaagat ctcaaagcag ttatgactag caagggaaac
    14521 attcagatta aatctgccaa aaaagctgaa gttaaaagtt ctgaaaaaga aaaataaatc
    14581 ctataaaaaa ggagtagaaa atgaaatttt tctgcttctt tttttactaa aataactgta
    14641 atttcttgac agcttgccct ttgtccatta tga
//
LOCUS       DAVKNL010000047        14523 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVKNL010000047 DAVKNL010000000
VERSION     DAVKNL010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14523
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.047"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(122..1972)
                     /locus_tag="VW411_001529"
     CDS             complement(122..1972)
                     /locus_tag="VW411_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU9794241.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(2014..3171)
                     /locus_tag="VW411_001530"
     CDS             complement(2014..3171)
                     /locus_tag="VW411_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9794242.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKEDIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTETFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREYGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFILFSIHFAYGLGTIVGLIRGFKWKKEYKRTIIYLDKISQINQNML"
     gene            3229..4395
                     /locus_tag="VW411_001531"
     CDS             3229..4395
                     /locus_tag="VW411_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEU9794243.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPSVAALLLIDILMTVALFTVDRADKIL"
     gene            4534..4860
                     /locus_tag="VW411_001532"
     CDS             4534..4860
                     /locus_tag="VW411_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU9794244.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWALYTDTINGIIEGS
                     IRHDKN"
     gene            4847..5440
                     /locus_tag="VW411_001533"
     CDS             4847..5440
                     /locus_tag="VW411_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEU9794245.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYATRFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            5433..6344
                     /locus_tag="VW411_001534"
     CDS             5433..6344
                     /locus_tag="VW411_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEU9794246.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            6407..7471
                     /locus_tag="VW411_001535"
     CDS             6407..7471
                     /locus_tag="VW411_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794247.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADLDTPIEK
                     NTQLEEEVPQAEVELESQQEEKIEAPEDSEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(7649..8509)
                     /locus_tag="VW411_001536"
     CDS             complement(7649..8509)
                     /locus_tag="VW411_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEU9794248.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYEAIARLDYAKFLEVLPPSFYHQLDANQIEVQPILD
                     KDFKTLAQEK"
     gene            complement(8784..9770)
                     /locus_tag="VW411_001537"
     CDS             complement(8784..9770)
                     /locus_tag="VW411_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEU9794249.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(10042..11517)
                     /locus_tag="VW411_001538"
     CDS             complement(10042..11517)
                     /locus_tag="VW411_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEU9794250.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDSAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWENIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            11561..11701
                     /locus_tag="VW411_001539"
     CDS             11561..11701
                     /locus_tag="VW411_001539"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794251.1"
                     /translation="MILYSIKIKEQTRKLAAGCSKHCFEVVDKTDEVSYIYLRQGDVD
                     AV"
     gene            complement(11775..12698)
                     /locus_tag="VW411_001540"
     CDS             complement(11775..12698)
                     /locus_tag="VW411_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9794252.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLIGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(12712..13641)
                     /locus_tag="VW411_001541"
     CDS             complement(12712..13641)
                     /locus_tag="VW411_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9794253.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(13960..14181)
                     /locus_tag="VW411_001542"
     CDS             complement(13960..14181)
                     /locus_tag="VW411_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794254.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSQKELQKAVLF
                     LADEKGSEHTAAELIDNLKEVIAKLKANA"
     gene            complement(14135..14395)
                     /locus_tag="VW411_001543"
     CDS             complement(14135..14395)
                     /locus_tag="VW411_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794255.1"
                     /translation="MTGVSNPTTDSARLVLAEANKAFADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
BASE COUNT         4330 a         3124 c         2679 g         4390 t
ORIGIN      
        1 aaccatatac ggcaaggcga cgttgacgtg gtttgaagag attttcgaag agtataagtc
       61 tctacataat aaaacgcata ttaccaaggt tgtgtaactt gatactatgc gtttttcttt
      121 tttattcaat gtgggttgtt tgattagcaa aggcgataat agcttgcttc aactcatctc
      181 cactgagagt gcggaactct ccaatctttt gattgatgga ttctaaaggc atgacattaa
      241 ccttaccaac gaaaatctta tccataactt gattatcaac gagttcggtt gataccaaga
      301 gttcttcgta gagtttttca cctgggcgga ttccaacttc aacgattgga atttcacttt
      361 cagtgtggcc acttagaagc accatcttct tggccaagtc ataaatcttg actggtttgc
      421 ccatatcaag gataaagact tccccatctt tggcataagc accagcatgg ataaccagac
      481 ggctagcttc tggaatggtc ataaagtaac gggtcatacg gaagtctgtc accgttacag
      541 gcccaccttc agcaatctga cgttcaaaga ctggaatgac actaccacgg ctaccaagaa
      601 cattcccaaa acgaactgca cagtaggttg attggctacg ttggttaaag ccagtgacaa
      661 tcaactccgc cacgcgcttg gttgctccca taacatttgg tggattgact gccttatctg
      721 tcgaaatcat aaccatctta gacactttag cttcatcaac agccttagca acattgtaag
      781 ttccacggat attgtttttg aaggcttctt ttggattgcg ctccatcata ggaacatgct
      841 tgtgggctgc cgcatgataa acaatagcag gtttgtactg ctcaaagact tgcaacaaac
      901 gatcatagtc ttgaatgtcc gcaatcacag gtacataatc aatcccttgg aacttacgaa
      961 tcaattcatg ataaacaagg tagattgagt tttccccatg accgagcaag acaatgcgtt
     1021 caggattgaa gcgactaact tgacgacaga tttcagaacc gattgaacct ccagctcctg
     1081 tgactaagat ggtcttacct gtcagttctg cacccagacg cgattcgtca agacggattt
     1141 cctgacgacc caaaaggtcc gtaatatcaa ttttttggaa gccagtacct gcttggtgaa
     1201 ggccctgaac aacagtttca accttaggca tcttgtaaca tttgacaccc agcttattac
     1261 acatctgcaa gatacgctca tattctgacg gatccagcga cggaatcgca acgatgacac
     1321 gctcgatttg atggcgtttg gctaattcag gcagattgtc ataagagccc aaaacaggaa
     1381 taccaccaag tttttgaccc tttttcttag aatccttatc caaaataccg accagttcta
     1441 attcactggt tggatgttgg taactatcca taaaaagagc cccaccatca ccggcaccaa
     1501 tcaagaaggt ccgacggtgt tctccatcac cactaccttt tttgcgtctg gagtagatta
     1561 actgccaagt aatccgtggc aataaaatca agaaggtact caacaagata aagagaatga
     1621 tgaaacggat ggagaagagt ggcaagaagg cataacagat actatatgac aagacactgc
     1681 tagcagtcac accaaaaaag attttcatga aatccgtaat cttgctgtaa cgactaatgc
     1741 tcgcgttcaa cccccaaaaa ccaatcatca attgatagaa caggaaggcc aaactcgtat
     1801 agataatgta gtcaacaggt gctggattaa tcagcccata aaataaaata taagatacaa
     1861 tgatggaaac caccatactg aaaatatcaa atattcccca gaaaacctgc ttttgttgtt
     1921 tatttaaaat ttccaccaga tcaatcacat aatctgttag ttttttattc atagaaatct
     1981 actcctcctt aaaagagttt tactatattg ttattatagc atattttgat ttatttggct
     2041 tattttatcc aaataaatta ttgttctctt gtactccttc ttccatttaa atcctctaat
     2101 taaacctaca atcgtcccaa ggccataagc aaagtgaatg gaaaataaaa taaagggcat
     2161 cacaattaga aatccatttt tatgttttaa taaagtcagc aaagtgagta atgacaaaag
     2221 tagaaaatag gcacctaata gtaaagttat gaatacgaat gtgatcggta acaatgctag
     2281 actaaacaca agactcaaaa caaataaaca aggaacatag tgaaataatg ataaacactt
     2341 aaactgaaca tgacttgtca agccaatcca caaaccattt gaatactttt gatgcagcat
     2401 tttcttgaat gttggtcgaa tatactgata agatagaata cttgggctat agcggatttt
     2461 ataaccatat tctcgaattc tataatgaat atcattatct tcagttcggc caagttgctc
     2521 atttactaaa ccaaccttct ggaaaacctc tcgtttatac attccatgaa aaatagaaga
     2581 aacatatcta tcctcagaac tatttcgata attggcaatg ctactgccaa acatattttc
     2641 ctcaacaaga tgcaaggtct ctgcccattt tccttttcct tcgacaatcg tcggtctagg
     2701 ccccccacag acaaattcac cttgttgaat aatagccaca ttgttcatta caaaagtctc
     2761 agtaactttt gaatgagcat caatttttaa aataaggtcc cctacagaat gtttaactcc
     2821 caggttaaaa ccactagctt gatttttctt aggattgtta tacaatctaa ttgagttaaa
     2881 ctctgtatct tcctttataa attgctgaat gatagctgtg gtcccatctg tggacatagc
     2941 atttataaat agaatttcaa tatcctcttt aggataggtt tgatttttta agtcttcaat
     3001 tagaccaggc agatattttt cttcattgta tgcactgatt acaatggata cgagaagact
     3061 ttcaacattt tgagtcacta ctactctcct gaatttttcc ttccaccatt ctatcataat
     3121 tttcaagact ttcccatttt tatcttgaaa gccagaaacc ttacttgaca ttatagtgaa
     3181 aaagccttaa aataagctgt tattaaaatc agctagaaga ggtattatat gaaaaagcaa
     3241 tcactctttt ttgttccagg tattatcctg attggtgttt ccttgcgaac tccttttact
     3301 gttttaccca ttattttggg aaatatttcg caaggtctgg aggtagaagt tagttcgctt
     3361 ggtgtcttga ccagcctgcc tctccttatg tttaccctct tttcaccatt ttctacccaa
     3421 ctggctcaga aaatcggctt ggagcatctc ttcacctaca gcctcttctt cttgaccatc
     3481 ggctcactta ttcgactaat caatctgccc ctgctctatc taggaacctt gatggttggg
     3541 gcaagtgtcg cagtcatcaa tgtcctgctt cctagtctta tccaagccaa tcaaccaaag
     3601 aaaattggtt ttctgaccac cttatatgta acgtctatgg ggattgcaac ggctctggct
     3661 tcctatctag ctgtgcccat tacacaagcc agttcttgga aaggacttat ccttctcctc
     3721 acgttactct gtctagcaac ttttttggtc tggctcccaa atcaccgcta taatcataga
     3781 ctagctccac aaaccaaaca aaaaagtcaa ataaaggtca tgcgtaataa acaggtttgg
     3841 gcaattatta tcttttcagg ttttcaatcc ttgatctttt acaccgtcat gacctggtta
     3901 cctaccatgt ctatccatgc aggtctatcc agtcacgaag ctggcttgct gacttctatc
     3961 ttatctctga ttagcattcc tttttcaatg accatcccaa gcctgacaac cagtttatct
     4021 actcgcaacc gtcagctcat gctcactctg gtttcactag ctggtgtggt cggcatttcc
     4081 atgctctttt tcccaatcaa taatttcatt tactggcttg ccatccatct cctcatcgga
     4141 accgcaacca gtgccctctt cccttatctc atggtcaact tttcactcaa gacaagcgcc
     4201 cctgaaaaga cagcccaatt gtccggccta tctcaaacag gaggctatat cctagcagcc
     4261 tttgggccaa ccctctttgg ttacagtttt gacctgttcc actcttgggt accatctgta
     4321 gctgccctct tgctcatcga tatcctgatg actgtggccc tctttacagt ggacagagcg
     4381 gataagatcc tttaaacttc tagttttagc tagaagtttt ttatatataa aaattttaca
     4441 catttctctt gacagggctt tcttttagat gtacaatgta tgtgtagaaa aattatatat
     4501 aaaaatctta cacattagaa aaggaggttt cccatgtact ttccaacatc ctctgccttg
     4561 attgaatttc tcatcttggc tgtactggag cagggtgatt cttatggtta tgagattagc
     4621 caaaccatta agctgatcgc taatatcaaa gaatccacac tctatcccat tctcaaaaaa
     4681 ttggaaggca atagctttct gacaacctat tctagagagt tccaaggtcg catgcgcaaa
     4741 tactactcct tgacaaacgg tggtatagag cagctcttga ccctaaaaga tgaatgggca
     4801 ctctatacag acaccatcaa tggcatcata gaagggagta tccgccatga caagaactga
     4861 atacctgact cagctagaac tctatctcaa gaaactacct gaagctgacc gtatcgaagc
     4921 catggactat ttcagagagc tctttgacga tgctggagtc gaaggagaag aagaactcat
     4981 cgctagtttg ggaactccca aagaagcggc ccacgaagtt ctatccaatc ttctcgataa
     5041 aaaaatcaat gaagcacccg ctcaaaaaaa taaccgacaa attttacata tcgccttgtt
     5101 agccctcctt gcagcaccta tcggcattcc tctgggaatc gccatcctcg tgaccctgtt
     5161 cgcaattctt gtagccgctt tgactgtcat tctggctttc tttgcagttt ccatactggg
     5221 tatcatcggc ggattcctat ttttagttga aagtttcact gtcctcgccc aagccaaatc
     5281 agcctttatc ttgatttttg gttctggttt actggctatc ggtgcttctt cgctagtttt
     5341 acttggcatt tcctatgcaa ctcgcttctt cggtctactc attgttcgtc tggtacaatt
     5401 tgttcttaaa aaaggaaaga gaggtaatca gcatgcgtaa atggacaaaa ggatttctca
     5461 tctttggtgt ggtgactacc gttatcggct ttatcctgct ttttgtaggt atccaatctg
     5521 acgggattaa gagcctactt tccatgtcca aagaacctgt ctatgatagc cgtacggaaa
     5581 agctaacctt tggcaaggaa gtcgaaaacc tagaaattac tctccaccaa cacacgctca
     5641 ccatcacaga ctctttcgat gatcaaatcc acatttctta ccatccatct ctttctgctc
     5701 accatgatct tatcaccaat cagaacgata gaactctgag tctcactgat aagaaactgt
     5761 ctgaaactcc gtttctctct tctggaattg gtgggattct tcatatcgca agtagctact
     5821 ctagtcgttt tgaagaagtt attctccgac taccaaaagg gagaactcta aaagggatca
     5881 acatctcagc caatcgcgga caaaccacca tcataaatgc tagccttgaa aatgcgaccc
     5941 tcaatacaaa cagctatatc ctccgaattg aaggaagtcg tatcaaaaac agtaaactca
     6001 caacgcccaa tatcgttaat atctttgata cagttcttac agatagtcag ctagagtcaa
     6061 cagagaatca cttccacgct gaaaatatcc aagtccatgg caaggttgaa ctgactgcca
     6121 aagattatct cagaatcatc ctagaccaga aagaaagcca acgaattaac tgggacatct
     6181 caagcaacta tggttctatc ttccaattca caagagaaaa gcctgaatca agaggtacgg
     6241 aattaagcaa cccttacaaa actgaaaaaa ccgatgtcaa ggatcaactc attgcgagat
     6301 ctgatgataa tattgatcta atatccacac caagcagacg ttgacaaaaa cgcttacatt
     6361 tgctactatg gaaacatatt tatctaacta aaaaggaggt tctaccatga aacaagaatg
     6421 gtttgaaagt aatgattttg taaaaacaac aagcaagaac aagcctgaag agcaagctca
     6481 agaggttgca gacaaggctg aagaaacgat agccgatctc gatacaccaa ttgaaaaaaa
     6541 tactcagtta gaggaggaag tccctcaagc tgaagtcgaa ttggaaagcc agcaagaaga
     6601 gaaaattgaa gctcctgaag acagtgaagc gagaacagaa atagaagaaa agaaggcatc
     6661 taattctact gaagaagagc cagacctttc taaagaaaca gaaaaagtca ctatagctga
     6721 agagagccaa gaagctcttc ctcagcaaaa agcaaccacg aaagagccac ttcttatcag
     6781 taaatcttta gaaagtcctt atatccccga ccaagctcca aaatctaggg ataaatggaa
     6841 agagcaagtg cttgattttt ggtcttggct agtggaagcg atcaaatctc ctacaagtaa
     6901 gttggaaaca agtatcacac acagttacac agcctttctc ttgctcattc tgttttctgc
     6961 atcttccttt ttctttagta tctatcacat caaacatgct tactatggac atatagcaag
     7021 cattaacagt cgcttccctg agcagctagc tcctttaact cttttttcta tcatctctat
     7081 cctagtagcg acaacactct tcttcttttc attcctcttg ggtagtttcg ttgtgagacg
     7141 atttatccac caggaaaagg actggacgct agacaaggtt ctccaacaat atagtcaact
     7201 cttggcaatt ccaatcttcc tcactgctat tgctagtttc tttgctttct ttgatagcct
     7261 acgatttaca gccctcttgt gtgtgattag cattggaatc attctgcttg ccagtctcca
     7321 tatcattaca agaccaagtc aagcaagtga aaccgactcc ttctatcaat tattcttgtc
     7381 tgtccttgtg aacggagtta ttatcctcct cttctttgta gctgaagtgg cactgattgg
     7441 agattatctt cgtatcttgg cctttcttta aacttcatac tcttcaaaaa tcaaattcaa
     7501 accacgtcag cttcgcctta ccgtactcaa gtacagcctg cggctagctt cctagtttgc
     7561 tctttgattt tcattgagta gcaatcctgt catccttggc aggatttttt ctatgtcaaa
     7621 aaagcaagtc gattgacctg cttctgcttt acttttcttg tgctaatgtt ttaaaatctt
     7681 tgtctaatat gggttgcact tctatttgat tggcgtctag ttggtggtaa aaagatgggg
     7741 gtaatacctc taagaatttt gcatagtcca agcgagcaat ggcttcatag tcttctggtt
     7801 tagagccatc tcctgtgatt tgaaccaagt caatctccca ctggacttcc ttatccacca
     7861 attgctcaat ataggctgca ggaacaaacg gttctctcgg taaaactgtc ttgactcctt
     7921 gagctagatg gtaaacgcca tgcacttttc cttctccatc ctgatagaag gaaactctcg
     7981 caaagtaggc atctgtctct tgaaccgcac gcacacgcgc ttcaaactga gccaagccat
     8041 ctgcctctaa aaagcttttc aagttctcac gactaaagaa aataggatta aaaattcctt
     8101 ggcaaatcgt aaaacgagct acagggtctg ctagataggc ttgaatactt gcttggaaat
     8161 aagcttcaaa atcaaaacca tctagccctt caatatcagt ccctgtataa gcaagcaaag
     8221 catctaaaaa tgacttgata atctgccagt ctgtctttgt tagtaggtca ggaagatcga
     8281 cacggtaagc cttttgacca tcagcataac tgaccttaaa gagaaattgt gattgcccca
     8341 ctatcgcaca ctcgatatat tcgagacgat taagaggctg acggagatag actacatcat
     8401 agctatgtga ctccaaaccg tctagcaagg ccaagatcga cttggcattc aaaatctctt
     8461 gttctcctaa aatgctctgt ttatttggaa taaaaaatgt tttcgccata actggactcc
     8521 tttgaaatcg tttacataca gtatacctta tttttctgaa agatacaaaa acaaacttta
     8581 tactcttcga aaatctcttc aaaccgcgtc agcttcgcct tgccgtagat atatgtaact
     8641 gacttcgtca gtcttatcta caacctcaaa gcagtgcttt gagcagcctg cagctagctt
     8701 cctagtttgc tctttgattt tcattgagta ttagattttt gagtacaaag catagaaaaa
     8761 cagcccctga ggactgtttg atcttataca gtagctgctt gatccaagct ttcaccgata
     8821 gcggctaggc gctcgataac ttcagcttgt gtcaattcat tttttgaaac atagcggtta
     8881 cgtgggtgaa cacggcactc gtgtgagcat ccacgaaggt acttgtcttc attttcttct
     8941 gatgtcaaga tacgacggtt acagaatgga tttccacagt tgacataacg ttcacatggt
     9001 gttccatcaa accagtcttt ccctacgata gttgggttga catggttgac atcaacggca
     9061 atacgctcgt caaagacgta cattttccca tcccaaagct caccttgaac ttctgggtct
     9121 ttaccgtaag ttgcgattcc tccgtgcaat tggccgacat ctttgtagcc ttcacggacc
     9181 atccagcctg agaatttctc acagcgaacg ccacctgtac agtaaaccac gacacgcttg
     9241 tccatgaatt tttccttgtt atcacggacc cattgtggta actcacggaa gttgcgaata
     9301 tctgggcgaa tagctccacg gaaatgtcct aggtcgtact cataatcgtt acgtgtgtca
     9361 aggacaacgg tatctttatc aagaagcgct tctttgaact cttttggaga caagtaagca
     9421 cctgttgttt caagtgggtt gatgtcattg tcaaagtcgt tgtcttccaa accaaggtgg
     9481 acaatttctt tcttgtagcg aacaaacatc ttcttgaagg cttgttcatt ttcttcgtca
     9541 atcttgaacc agagttcttc cattcctgga aggctgtgaa cgtagtccat gtatttttga
     9601 gttgtttcat agtcacctga aactgttccg ttaattccct cgtcagcgac taggatacgg
     9661 cctttaaggc cgattgattt acagaaagcc aagtggtctg cagcaaattg ctctgcattt
     9721 tcaattggag tataaaggta gtaaagtaag acacgaatat cttttgccat aagatttgtt
     9781 ctcttttcta ttcttaaatt ttctaaattt tttatttatc tacactagta tacctgcttg
     9841 agaaaacgaa tgcaatttat ttgactggaa attgtagagg gttctcgaaa tttcttgaat
     9901 ggttaaaata aggacaagag aaaacatgga tatctatatc cttgtgccaa aaaaaccact
     9961 gccctcccca gaccaacctg aggaaagcag tgattcttat tttaggagtt aggaatgaat
    10021 acacgaaatc aatttagctg attatttttt gtttttcaag aattcatcgt attgtttttg
    10081 catttcgttc aatacttttt cgtaggcacc ttcagatttc aatttttcca tcaattctgg
    10141 aatcgcttta tctgggtcta cagtaccagt gttgatagct gtatcaaatt gttgcattgt
    10201 gttagcaata gctgagattt cagatttcac attgtcagta ttgaagataa atccaagcgc
    10261 tggagattct ttagcttctg ccaattcttt cttagaattt tcgatttgtt ggtctgtaac
    10321 gttttcgttg atgtaaagga tccagttgtt accagtgttc catccaccca tgtgagtgtt
    10381 tcctttgtag ccatcaagaa cgcgaacacg gttttcttta ccttcaatgt tttcccagtt
    10441 cttgccttct ggaccgtaaa caagaccgtt caagagttct gggttcgtat tcaagaggtt
    10501 caagatttcc attgattttt ctttgttctt agagttgttt gagatgacaa agttagcaac
    10561 ttgtgttgtt tggtttttct tgatgaagtt agtaattggt ttgatttgga tatctttgtt
    10621 ggcaacacgt gaaagcaagc tgttaccgta gtcagctggt cctactgttt cttcacgaac
    10681 gaaccaagta tcttgttgaa ggtcaaagga agtatcgctt gttgcgacgt cttttggaat
    10741 gtagccagct tcatagaatt tgtgaagagt cttcaagtgt tctttgaaac gaggcacttc
    10801 gtaacggttt acaactttag tagtatcgcc ttcaaggtcg ataacgaatg gaagaccgtt
    10861 tgctactggg tagtcaaaat tatcagatgg gatgaaaact ttaccaatag caaatggtac
    10921 tacgtctgga gctttttctt tgatttgttt caagactggc tcaagagttt cgtaagaagt
    10981 aacacctgaa atatcgatac catatttagc aaggagagtt ccgttgaagg caaagttttg
    11041 agatgatgca acgttggctg caactggaac agcgtaaatc ttaccattta cagtattacc
    11101 cttgatgtaa gctgagtcaa gtgctttgta aaggtcttta ccttcttttt tgtacaattc
    11161 tgtcaagtca gcgtaagcac ctttttgagc atttacaata tagttatctg caaaggcaat
    11221 atcatagttt tcaccagatg atgtgataac tgacattttc ttaccatagt caccccagcc
    11281 aaggtattgg atatccaatt tggcaccaac tttttcttca atgattttgt tggcatttgc
    11341 taacaattca tccaagttgt ctggtttgtc accgatttgg tacattttga taacaggttt
    11401 gtcacctgaa tcagcagctt ttttgctgtt acctgtcaaa tttccacaag cagcaagacc
    11461 tgcagccaga gcgactacac tagcagatgc aaaagcatat tttttccagt ttttcatgat
    11521 aaaaactcct ttttttattt ttaaacttat aaacaatgta atgatcttat actcaataaa
    11581 aatcaaagag caaactagaa aactagccgc aggctgctca aagcactgct ttgaggttgt
    11641 agataagact gacgaagtca gttacatata tctacggcaa ggcgacgttg acgcggtttg
    11701 aatttgattt tcgaagagta ttaacttcac acaagggaag ttgggaactg agaaatgtta
    11761 tttctcaata agcactattc tttcacacca ccgatagtca aaccttttac aaagtagcgt
    11821 tggaaaaatg gatacaaaat cgcgattgga agggttgcaa ccacaaccat ggccatacga
    11881 cctgtttctt tcggtagagc aactcccagt tgaccaatca agccgaccgc tttggcaatg
    11941 tagtccatat tttgttggat ttgcatgagc aaatattgca atggatacaa gttgtcactc
    12001 ttgatgtaaa gaagggcgtt gaaccagtca ttccagaaac caagagctgt taagagcgtg
    12061 atggttgcga tacctggtag tgacaatggc aaacagattt ggaagaaaat ccgggcctca
    12121 ctggcaccat cgatacgagc cgattctaga atggcttctg gaatggtctt cttgaagaag
    12181 gaacgcatca agatgatgtt aaatggtgag agaagcattg gaacaatcaa ggcccaaaca
    12241 gtgtcaccaa gctgaagtac acgggtcacc atgatataac ctggtaccaa accagcgttg
    12301 aacaacatac tgagaaggac gaagatggta aagaatctgc gatacttaaa ggttgtccgt
    12361 gaaatagcgt aggcataggt tgttgtgata aagacatttg tcaatgtccc aactacggtt
    12421 acaaagacag agatgaagag ggcttgtagg attttatcct taaactgtgc caaaaactca
    12481 aaaccgtcta agccaaattg ggatgggaag aagctatagc cgtattggag gaggcttttc
    12541 tcgtctgtca ctgaaataat gataacgaat acaaaaggta ggatacaaga gagggcaatc
    12601 aaacccgaaa tgatactgaa gaagatatct gctttcttac tgaaggagtg aatgccgaca
    12661 ttatcaattt tttctttttt aattttcttt tttgccatat tctcctcctt tctagaacaa
    12721 agctgagttt ggatcgactc gtcttgcaag caagtttgat aggataacca gaatcaaacc
    12781 aacaacggat tggtaaagac cggctgctgc agccataccg atatctgctg tctgagtcaa
    12841 accattaaag acatatacgt ccaaaacgtt ggttacattg taaagctgac cagcattgtg
    12901 tgggatttga tagaagagac cgaagtctgc gcggaagata tttccgactg caaggatggt
    12961 caatacagtt acaagcggag tcaactgagg aatggttacg ttgcgaatac gttgccactt
    13021 gctagctccg tccactgtcg ctgcttcgta gtaggttgga tcaattccca tgatcgtcgc
    13081 atagtacatg acactgctat atccaaagcc tttccaaata cctaggaaaa gtaggagata
    13141 gggccagatg cccaggtcag cgtagaaatt gacttctttg agaccaagac tttccaatag
    13201 atgattgaac acccctttat caatatttag gaaggcatct gtaaagaaac tgatgataac
    13261 ccaagacaag aagtaaggga acaacataga agtttgaaaa atcttcacca ttctcttaga
    13321 acggagctcg ctgaggataa tggcaatccc tacagataca actaaaccta gaaagataaa
    13381 gccaagattg tagaggacag tatttcgtgt gataataaag gcgtctcttg aactaaataa
    13441 gaatctaaaa ttatcgagtc cgacccattt actatttatg atactatcta tgaaaccatt
    13501 actggtcatg tggtagtctt tgaaggcaac cacgttccca aatactggaa tgtaaaagaa
    13561 tagaatcaac cagagtgccc ctggcaaaac catcaagaga aagatccagt tgtctctcaa
    13621 tgtttttgaa aactttttca taatttcctc cctttttatt ttgatatcca tctaaaaatt
    13681 cttttttaga cttttgataa cgattacatt attagtatac tcctatttgc aggttaggtt
    13741 aaactcctaa ttatagaaaa aactccacaa attatgtagc agatttaaaa ctttatcacc
    13801 actatcaaac aaatgtccta aatcaattgt ttattttatc tctattagcc cagtgatggc
    13861 gtcactctgt tataagcatc caacaacggg gtatactgaa aaatctccag actagggaac
    13921 tcagcgatag ttcctaatct ggagattttt aatatgttat taggcgtttg ctttcaactt
    13981 agcaataacc tctttaagat tatcaatcaa ctctgctgca gtatgctcag agcctttttc
    14041 atctgccaag aacaaaactg ctttttgaag ttctttttga gagttttcaa ggacatcctt
    14101 atctactgtt tcaaggtttg agtctttaag aagtttactt aattccttgg ctaatttctt
    14161 gagtttgatt tgcagactca tcttctcctg ctgtttcttt gcccgctgtt tgtcctccat
    14221 ccttagttgc tgactggctt tccttaatgg actctaggga agcaatggca tctttgactg
    14281 tttgcaagat atcacgtaaa ccttgctctg tcaaactatc atctgcaaaa gctttattag
    14341 cctctgccaa aaccagacgt gctgaatctg tggtaggatt cgatacacct gtcaatgatc
    14401 tcaaaagatt ttctaaggtt tgagtctgct tactaatact agactaaaat caaaaagtat
    14461 tatataacag tgatatgaaa tcaactaaag aagaaatcca aaccatcaaa acacttttaa
    14521 aag
//
LOCUS       DAVKNL010000048        14146 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVKNL010000048 DAVKNL010000000
VERSION     DAVKNL010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14146
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.048"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            1..1105
                     /locus_tag="VW411_001544"
                     /pseudo
     CDS             1..1105
                     /locus_tag="VW411_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1191..2393)
                     /gene="rpsA"
                     /locus_tag="VW411_001545"
     CDS             complement(1191..2393)
                     /gene="rpsA"
                     /locus_tag="VW411_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEU9794256.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFVDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(2555..2626)
                     /locus_tag="VW411_001546"
     tRNA            complement(2555..2626)
                     /locus_tag="VW411_001546"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2592..2594),aa:Gln,seq:ttg)
     gene            complement(2636..2716)
                     /locus_tag="VW411_001547"
     tRNA            complement(2636..2716)
                     /locus_tag="VW411_001547"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2680..2682),aa:Tyr,seq:gta)
     gene            complement(2766..2996)
                     /locus_tag="VW411_001548"
     CDS             complement(2766..2996)
                     /locus_tag="VW411_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEU9794257.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(3164..3808)
                     /gene="pcp"
                     /locus_tag="VW411_001549"
     CDS             complement(3164..3808)
                     /gene="pcp"
                     /locus_tag="VW411_001549"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEU9794258.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(3823..4746)
                     /locus_tag="VW411_001550"
     CDS             complement(3823..4746)
                     /locus_tag="VW411_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEU9794259.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(4743..5432)
                     /locus_tag="VW411_001551"
     CDS             complement(4743..5432)
                     /locus_tag="VW411_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEU9794260.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            complement(5497..6201)
                     /locus_tag="VW411_001552"
     CDS             complement(5497..6201)
                     /locus_tag="VW411_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9794261.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASHLNIIDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPVVSNVVPFSKPYSQVGIKGDPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKHIK"
     gene            complement(6257..7279)
                     /locus_tag="VW411_001553"
     CDS             complement(6257..7279)
                     /locus_tag="VW411_001553"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEU9794262.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            complement(7411..9882)
                     /gene="parC"
                     /locus_tag="VW411_001554"
     CDS             complement(7411..9882)
                     /gene="parC"
                     /locus_tag="VW411_001554"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEU9794263.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVIIEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            complement(10302..12245)
                     /gene="parE"
                     /locus_tag="VW411_001555"
     CDS             complement(10302..12245)
                     /gene="parE"
                     /locus_tag="VW411_001555"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEU9794264.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTNE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVVNTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEETTVF"
     gene            12381..13022
                     /gene="plsY"
                     /locus_tag="VW411_001556"
     CDS             12381..13022
                     /gene="plsY"
                     /locus_tag="VW411_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEU9794265.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            complement(13065..14146)
                     /locus_tag="VW411_001557"
                     /pseudo
     CDS             complement(13065..14146)
                     /locus_tag="VW411_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         4059 a         3163 c         2484 g         4440 t
ORIGIN      
        1 cttagaaagc gtcgtttcaa gtgctatcac tgttcaaaaa tgatggtcgc tgaaacttct
       61 atcgtcaaga aaaatcacca aattcctcgt attatcaacc aaaaaattgc gcaaaagttg
      121 attgagaaga tttctatgac cgatattgct catcagctgg ccatttcaac ttcaactgtc
      181 attcgaaagc tcaatgattc tcactttgag catgattttt cgcgtcttcc tgagattatg
      241 tcctgggacg ttgaaacagt ccggggagtg actgtttcaa tcgggagatg aagatgagct
      301 ttattgcgca agattttgac aagctcgata tcatcactgt tcttgaaggt agaacacaag
      361 ctatcatccg agatcacttt cttaaatatg atagagccgt ccgatgtcgc gtcaaaatca
      421 ttactatgga tatgtttagc ccttactatg acttagctag acaacttcgc tttcaaattt
      481 ctaggctcag gctgaaacag tctcccagac tgtttcactc ccgaatgcta aaatcgttct
      541 tgatcgcttt catattgtac aacatcttag ccgtgctatg agtcgtgtgc atgtccaaat
      601 catgaatcag tttcatcgaa agtctcatga atacaaggct atcaagcgct actggaagct
      661 catacaacag gatagtcgta aactgagtga taagcgattt tatcgtccta cttttcgtat
      721 gcacttaacc aataaagaga ttttaaacaa gcttttgagt tattcggaag acttgaaaca
      781 ccactatcag ctctatcaac tcttgctgtt tcactttcag aataaggaac cggagaaatt
      841 tttcggactt attgaggaca atcttaagca ggttcatcct atttttcaga ctgtctttaa
      901 aaccttcctc aaagataaag aaaagcttgt caacaccctt caactacact attctaacgc
      961 caaactggaa gcgaccaata atctcatcaa acttatcaag cgcaatgcct ttggttttcg
     1021 gaactttgaa aacttcaaaa aacggatttt tatcgctctg aatatcaaaa aagaaaggac
     1081 aaaatttgtc ctttctcgag cttagctttt cttcaaccca ctacagttga caaagagcca
     1141 gaaaaatccc ttgtttagta aacaaaggat tttgtgaatt ttcaatttga ttaaagttcg
     1201 atatcaccaa acaaatcagc cattgaaaat cctgtttgtg tttctggaag ttcgaaatca
     1261 cgcttttctt gacgtcttgg acgacgtgga cgagcagcac gtttttcttc tttttgtcct
     1321 tcttcttggg ctggacgttc ttcaagagct ttaatagaaa gtgacacgcg ttctgcatct
     1381 gcgttaactt caagaacttt aacttgaact tcttgaccaa ctttaagagc ttcttttgga
     1441 ttttcaatcc gtttgtgtga aatttgtgat acgtgaacaa gtccatcgat acctggcaat
     1501 acttcaacaa atgcaccgaa gtcagtcaaa cgtttaactg ttccttctac tacatcacct
     1561 ttagccaatt tttgctcaac gccatcccat ggtcctggta ctgttgcttt aagtgaaagt
     1621 gatacacgtc cttcttcttc gttaagatca aggattttca cttcaatttc ttcaccaaca
     1681 gttacaactg attttggtga tacattacgt tcatgtgaca attcagtcaa gtgaaccaat
     1741 ccgtcaacac caccaaggtc gacgaaagcg ccgaagcttg tgatacgagc aactttacca
     1801 gttacaacat caccaacagc caatttaccg aatacttcag cgcgagctgc tgcagtagct
     1861 gcttcaacaa cttcacgacg tgaaaggatg aagcggtttt ctttagcgtt aacttctttg
     1921 attttagtat caaattcttg acctacaaaa cgctcagcgt tacgtacgaa acgagtatcc
     1981 aacattgaag ctgggataaa tccacgaaca ccttcaaatt ctactgaaag tccaccttta
     2041 acggcacgcg ttcctttaac agtaacaact tcttcttcgc gaccaacaag tttgtcccat
     2101 gctttgcgag cttcaaggcg ttttttagat acaaggtatg taactgtatc agtatcttta
     2161 ccaactactt gacgaagtac aagaacatcc aatacttctc ctactttaac aaagtcattg
     2221 atatctgcat cacgatcgtt tgtcaattcg cgaagagtca agacaccttc aacaccagtt
     2281 ccagagattg caacgttagc ttgagtcgca tcaactgtca atacttcagc actaacaaca
     2341 tcaccagtct caacttgact aacgctattt agcaaatctt caaattcgtt catctaaaaa
     2401 atcctccaac aatcaagttt ttcttaaact tgacatactt ataatttttt tcctaagcac
     2461 ccgcaaggac atgttcatat cattcacgtc tttcaattat aaaaataaaa taccctaaga
     2521 tattttgctt tctatcttga ttattaccta agaactgggg tagctggatt cgaaccaacg
     2581 catgagggag tcaaagtccc ttgccttacc gcttggctat accccattaa tgaaatggag
     2641 agagagggat tcgaaccccc gaacccgaag gagcggattt acagtccgcc gcgtttagcc
     2701 tcttcgctat ctctcctaca atcaacatgg actattatat catgaaaatt tcaaaaaaac
     2761 aagacttatt ttgctaaatt ataattttca atcaaaattt ccacagcttt ttccaatttt
     2821 ttataaaaac tatcgacatt cccattcttt ataatggcaa attgtccgtc taattcggca
     2881 atttctccga taactttttt accgatagtc aatgtataac cttcaaaact gtctttacca
     2941 acattaactt tggcatccgc tacttgaatt tcgatttttt tatctttctt gctcatttca
     3001 tacctctctt cactttttct attttacaaa aaaatcctaa aactagcaag aaaaaactct
     3061 atatcaggct tctctgatat agagttttat gtaaacaacc tcaattgatt tagcatagtt
     3121 aatagttagt aaacaagcat aattgactat cctttttagg ttatcagtga gtctcaccgc
     3181 ctactaactg caaatcctta tctttgtagt ctacaatagc ttcaatagct acctctatgc
     3241 ctcttacaat atcacataga ctcattcctg cagtattcgg cttatttact acttgttcca
     3301 tcatatatgg aatatgcatg aaacctgctc tcatattagg gaatttttta tccactaaat
     3361 aaagagcttg gtacatcaaa tgattgcaaa caaaggttcc tgcactattg gatacaactg
     3421 ccggaagtcc ctgttttttg atagcttgta ccatcgcttt gataggtaaa ctactaaaat
     3481 aggccgatgc tccatcaata cgaatcggtg tatcaattgg ttgattgcct tcgttatcag
     3541 gtatgcgagc atcatcttga ttaatagcca ctcgttcagg tgttaagccg gtcctgccgc
     3601 ctgcttgtcc aatacaaagt acagcatctg gttgatatcg taatatttct gcctctaaaa
     3661 cttctgacga cttataaaaa accgttggaa tttctaccca gcgaacttca gccccattaa
     3721 tctcagatgg taataatttt acagcctcca aagctggatt aatcttttca cctccaaaag
     3781 gattaaaacc tgtaaccaat attttcattt tattttcctt tactaaaatg cgagaaagta
     3841 cattaagaat atgtgaataa caatcattac tagagcaaca cctgcttgag cctttataac
     3901 gccattctga tctttcatat ccatcaatgc tgctggtaga gcgttaaaat tagcagccat
     3961 tggggtcaat aaggtcccac aataacctgc tgtcatggca agagcaccag ccacaattgg
     4021 attagctccc agagcaaata caaagggaac tccaacacct gctgtaataa cggtgaatgc
     4081 tgcaaaagca tttcccataa tcattgtgaa tagaaccatt ccaagaacat aggccaaaac
     4141 tcctataaag cgactatctg aaggaacaat accgctaatc agatgagaga taacatcacc
     4201 aacacctgct acagtaaaaa tagcccccaa agcccctaat aattgaggaa caatcccact
     4261 tgttgaaact tgctgagtca ttcgattatt ttctgataac agactcttag ggtgactatt
     4321 ggtaatcaca agaacagaaa ttgtagcaaa caaggcggca aggctaatcg aaatcttgct
     4381 aaattctgga atcatttgcg ctaagaccaa cgcaagtatt gccatcagca taactggaat
     4441 aaaaatttta tttttcaacc tgttagattc aatattggct ttcatttcat ctaaggatgg
     4501 caaggttccg atacggactt gcttaaacaa tgttaacagc gataatagga ttacaataat
     4561 accaatactc atatttggca tataggaacc acctataaac gtaatagaca atagagtcca
     4621 aaatgcagat gtcccaagtc gaactgggtt tgttttatct ttataactac aataggctgt
     4681 atggagaaat tgacaaccaa tcacaatata ggtcaactct aatagttgct ttgccaactc
     4741 tgtcattttt gttctcctcc cctagtcttt tttgatatca attttttatc aaataaataa
     4801 ttataaatcc ccactacaat aagtgttata acagcaacaa taatagatgt agaagcaatc
     4861 cctgcataat tgctttcata gcctaactga tctaatgttc cccctatcaa gaggactccc
     4921 ccagcaccta caaacgtatt ttgagcaaag aaatttccaa aattttcatt cgcagccgca
     4981 cgcgctttta ttgtctcatc ttcaacctct gttaactttc tacctaattg agactctgca
     5041 gctgcttctc ccataggttg aaccaaaggt ctgacaaact gagggtgtcc tcctagacga
     5101 attgaaaaga aaccagctaa ctctcgaata aagaaataaa ctgtatagaa gtttccaact
     5161 gtcagacctt taatctttcg aatcaaatcg attgatcgtt gcttgagtcc aaaggtttct
     5221 gacagcccca caagaggcaa ggtaaccata aaaatcgtga gcactcgctg attgctaaat
     5281 tcttttccca aaatctccaa aaattcaacg agagaaacac ctgaaactaa agctgtaacc
     5341 aaaccagcta agactactgt tgcaattgta tcaaatttta aaataaaacc cacaacaatg
     5401 attgctattc ctattaatct aatccactcc atatcaaact cctttatatt caaaatgaca
     5461 gtatttttaa aattttatca agatcaatac cattccttat ttaatgtgtt tttctagttc
     5521 tttttggtat ttgctattgg attccaattt ttctttttgc cattttttaa aaacctcgtt
     5581 atattctttt gttgtaacaa tatctttttg caatttcatt cctttaaaga tatatggatc
     5641 ccccttaata ccaacttgtg agtatggttt tgagaatggt actacgttac ttacaactgg
     5701 agaaccacca gatgaagctg ttggcatcaa taatgaacta tctgtcgacc aagcttgagc
     5761 tttggcatat ttttcatatc ttttctctag gtcagtggtc tcagaaacag catcttctaa
     5821 caatttctta tatttatcca aaccaggttt agctacaaca tccttatctt ttcctttcgt
     5881 aataccaagg tgtttcatgg cagaaccaga ttttggatct ataatattca agtgagacgc
     5941 tggatcttga tagcttggag cccatcctgt actgttcaaa tcatagtctt tttgagaagg
     6001 agcaacattg ccgtatttat cattttccat caaaccatca ataacatttc caataacgtc
     6061 tgtcctcgat gttcgagtcg ctatactgta gcccaatgat gctggatcta ctgcatagac
     6121 ataagaaaat gttgtcggtg catctgcttc tttatcagtt tttccacaag ccactaaaat
     6181 agctgacgtg ctcaggacca ctcctgctgt taagagccac tttttctatt tcataaagaa
     6241 tctcctttgg tttattttaa tctactttta caatccaacc ttctggcgct tcaatatcgc
     6301 caaactgaat acccgtcaat tcattatata atttacgcgt cacaggacct acttctgttt
     6361 cactatagaa tacatggaaa tcatcaccat gttgaatacc tccaattgga gaaataaccg
     6421 ctgctgtacc acaggcacct gcctctacaa aacggtcaag attatcaatt ggaacatcac
     6481 cctcaatagg agttaatccc aagcgatgtt ctgccaaata aagcaaggaa tacttggtaa
     6541 tagatggcaa gatagatgga ctcaatggtg ttacaaattc attatcagct gtaattccaa
     6601 agaagttagc tgatccgact tcttcaatct ttgtatgagt tgatgggtcc agatagataa
     6661 catctgagaa atgacgtgac ttggccattt ttcctggtaa gagacttgca gcatagtttc
     6721 caccaacctt agccgcacct gtaccatttg gtgctgcacg gtcgtactca tcctgaatca
     6781 agaagttggt tgggaccaaa ccacctttaa agtaatttcc aactggcata gcaaagatgg
     6841 tgaaaatgta ctcttctgcc ggttttaccc cgataatatc tccgacacca atcaaaagag
     6901 ggcgaagata taaggttcca cctgttccgt atggtggtac gtattcttca ttcgcacgga
     6961 caactgcttt acaagcttct acaaacatgt ctgtcggaac ttgtggcatc aagagacggt
     7021 cacatgtacg ttgcagacgt ttagcatttt catcaggacg gaacagttga acactgccat
     7081 ccttagtacg ataagctttc aaaccttcaa atgcttgttg tccatagtga agacttggag
     7141 aagactctga aatatgcaaa gttgcatcct ctgtaagctc tccttgatcc cattgtccat
     7201 ttttgaaatg agcaagatag cgataaggta atttcatata ggaaaaaccg aggttttccc
     7261 aatcaatcgt tactgtcata ttccactcct tattctaaaa acctatttct tatattctac
     7321 actatttttc taaaatagca agtatatttt gtaattttca gaaaatttct ccaataaaaa
     7381 ccaactctta gaactgattc ttcatttcac ttatttatct tcagtaacta cttcctgaag
     7441 ataagcgtca aaaacttctt catctgaaat cgtgtcagaa atgaagcttc cattgctagt
     7501 gcgttctgac aagttcaagt cttgcaatcg gctttcatag attgttcctt tattggattg
     7561 gacaagcaga gtttggtcgt tcacatccac ttccgtactg aagaaatcgc caacaaatcc
     7621 ttgctctgca actgctcctg ccaagaagac acgatgcggt ttgtttttca actcacgcaa
     7681 gacttgtaat cctcgtttgg cacggctggt tgctagaatt tcctcaatgg aaacacgttt
     7741 caagcttcca cgctgggtca agaggtagaa ggacgaagta ttacagataa agccagattg
     7801 gaggacatca tcttctttca aattcatagc cttgacacct gctgccttag caccgacaac
     7861 cggaacctct tcgatattga aacgcagggc ataaccattt tgactaacca agacaacatc
     7921 atctagttta atcggagcca ctgctacaat ctgatctgta tcgtctttga gcttagcata
     7981 cttgacagac ttagatctat aggtccgcca tggagtgaat tcttttcgct ctacccgttt
     8041 gatttgacca aggcgagtca ctgcaaagta ggttgtcgca tcgtcaaact gatccagtac
     8101 ttccacataa aggatttctt cattcgtttc aaagtttgtg atggtttggc tcagatgctc
     8161 tccgatgtcc ttccaacgaa tatctgccaa ctcatggatt ggtctgtaga tgacatttcc
     8221 aagacttgtg aacatcaaga ggtgctgggt tgtcttggca gattgaacaa aaatcaaacg
     8281 gtcatcatca cgcttgccaa tttcttccaa ggtggaagcc gcaaaggaac gtggactggt
     8341 acgcttgatg taacctgcct tggtcacgct gacgtaggta tcttcctcag cgataagact
     8401 agctgtatca atctcaattg ctttcgcagt gtcttctaaa gaactcaaac gaggagttgc
     8461 aaatttcttc ttgacctcac gaagttcttt cttcatgaga ttgtacatag tcctttcatc
     8521 accgataata gccgccagca tagcaatctt ctcacgaagc tctgcttctt cttcctgcaa
     8581 gacaaccaca tcggtattgg tcaaacggta cagttgcaaa gttacgatag cctcagcctg
     8641 ttcttccgta aaatcatagc taactttgag gttttccttg gcgtccgcct tattctcaga
     8701 agcacggata agagcaatga cttcatccaa aatcgaaatc acacgaatca aaccttcgac
     8761 gatatggaga cgtttctcag ccttttcttt gtcaaagcgt gaacgcgcca aaatcacttc
     8821 tcgacggtga gcgatatagc tagacaggat tggaacaatc ccaacctgac gaggtgtgaa
     8881 attgtcaatc gccaccatat taaagttgta gttgatttgt aggtcggtgt acttaaataa
     8941 gtagttgaga acaagctcag tattagcgtc tttcttaagt tcgatagcga tacgaagacc
     9001 atcacggtca gactcatcac gaacctcagc aatcccagct accttgttat taacacgaac
     9061 atcatcgatt ttcttgacta gattggcctt attgatttca taaggaatct caataataac
     9121 gatttgttcc ttaccacctt ttagcttttc aatttcagtc ttggaacgaa caaccacgcg
     9181 ccctttccca gtctcataag ctttcttgat ttcatcacga ccctgaataa tagcccctgt
     9241 agggaagtct ggtccaggca agaattccat gagtttatca atctttgcag ttgggtggtc
     9301 aatcatgtaa actgcagcat ctatgacctc agctaaatta tggggaggaa tgtctgtggc
     9361 ataaccagcc gaaatcccag tcgaaccatt gaccaagagg tttggaaagg ctgctggcaa
     9421 gaccgttggt tctttctccg tatcgtcaaa gttccatgca aaaggaactg tctttttctc
     9481 gatatcctga agaaggtagc ctgcaatttc agacaaacgt gcctcagtat aacgcatagc
     9541 cgcaggagga tctccgtcca tagaaccgtt attaccgtgc atttcaacta gaatctcacg
     9601 atttttccag ttctgtgaca tacgaaccat ggcatcatag atagaagaat ccccgtgtgg
     9661 gtggaaattc cccatgatgt tcccgactga cttggccgac ttacggtagc tcttgtcaaa
     9721 agtattgcta tccttattca tagaataaag aatacggcgc tgaaccggct tcaacccatc
     9781 acgaatatct ggcaaagccc ggtcttgaat aatgtacttg gagtagcgac caaagcgctc
     9841 tcccatgatg tcctccaggg acatgttttg aatgttagac ataagataca aagcccataa
     9901 aataccaagt gaaaatagaa aattcttgaa gtaagcaaac tcacaagaga atttatcttt
     9961 ttcacacagt atctagggcg tgttcaactc ctttcaaaga atgtagagta ggtttttatg
    10021 cagtaaaaga tattttacgg gaattcctcc cgtgttcagt tacgataagt aaccaaacta
    10081 tcctgtttgt atttttcaat atgaaaatct ggttttccaa aattagtctt agtttgtgtc
    10141 ttagccgctc ccttaagcgc ctctttgaga taagcactca tagcagattc ttcattaata
    10201 atcctgcaat tttttcaaac caagattttc aaactgcttt ttcacatagt cattcacatc
    10261 cgactctaat ttccagttta ctaacatatt attttctttc attaaaacac tgtcgtttct
    10321 tctagcgtaa acttgacatt atcttcaatc catttacggc gtggttctac cttatctccc
    10381 atgagaacat tgacgcggcg ttcggcgcgc gctaaatctt caattgtgac acggatgagg
    10441 gtacgtgttt ctgggttcat ggttgtttcc cagagctggt ccgcattcat ctcaccaagt
    10501 cctttgtatc gttggagggt agcgccttta ccgaactgtt tacggagttc ttctagttct
    10561 ccgtccgtcc aagcgtaggc cacttcttct ttcttgcctt tacctttgga catcttgtaa
    10621 agaggtggga gggcaatata gacatgacct gcctcgacta gcggacgcat gtaacggtag
    10681 aaaaatgtca agagcaaggt ctggatatgg gcaccgtcgg tatccgcatc ggtcatgata
    10741 atgatcttat catagttggc atcttcaata gagaagtctg ctccaacacc cgcaccaatg
    10801 gtataaatca tggtattgat ctcttcattt ttgaggatat ccgccatctt ggccttggct
    10861 gtattgacaa ccttaccacg aagaggtaga atagcctgga acttgcggtc acgaccttgt
    10921 ttggcagaac caccggcaga gtccccctca actagataga gttcattctt agcaggattc
    10981 ttagattggg ctggggtcaa tttcccagac aacaagccct tatctttctt gtttttcttc
    11041 ccatttcggc tctcatcacg cgccttacgt gctgcttcac gagcatcacg ggccttgata
    11101 gccttgcgga tgaggttaga agctaattcc ccattttcca taaggaaaaa ggtcaactta
    11161 tcagccacta ttccatccac aactgggcga gctagggggc ttcctagttt atccttggtc
    11221 tgtccttcaa actgcaagtg ttcttcagga actaagatag aaagaacggc cgctagtccc
    11281 tcacgatagt ctgaaccttc aaggttttta tctttttcct tgagaagacc tgttttacgt
    11341 gcatagtcat tcatgacctt ggtaatggca gacttgagtc ctgtctcgtg cgttccaccg
    11401 tccttggtgc gaacgttatt gacaaaagat agaatgttat ctgagaatcc gtcattgtac
    11461 tggagggcta cttccacttg aaaaccattg tcttcccctt caaagtaaag aactggcgtc
    11521 aagatttcct tatcttcgtt gagataagaa acaaaatctt gtactccatt ctcatagtgg
    11581 aactcaatcg cttcatttgt tcgcttgtcc gttaaagaca aggtcacatt tttcaagaga
    11641 aaggctgatt cattaaggcg ctctgaaatg gtattgtact tgaaatctgt cgtagaaaat
    11701 atagtcgcgt caggcataaa agtaactttg gtgcctgttt tagacttggg tgctgtaccg
    11761 attttcttca aagtcgtgac aggttttcca ccattttcga aacgttgctt gtaaactgcg
    11821 ccatcacggg taatttcaac ttctaaccag ctagaaaggg cgttaacaac ggaagaaccc
    11881 actccgtgaa gtccacctga tgtcttatag ccaccttgac cgaatttccc tccggcatga
    11941 agaatggtaa agataacctc aacagttgga attcccatag cgtgcatacc tgtcggcatc
    12001 ccacgtccat ggtcttgaac cgttagacta ccgtctttat tgatagttac atcaatacga
    12061 tcaccaaacc cagacaaggc ttcatcgact gcattatcaa cgatttccca aactaggtga
    12121 tgaagaccag cgccatcggt cgatccaata tacatccctg gacgttttcg gaccgcatcc
    12181 aacccttcta gcacctgaat agcatcatca ttataattgt taatattgat ttcctttttt
    12241 gacacaagga acctcctatt cgttcatctt tactattcta caggttttcc aaggattttg
    12301 caaaattttt ctttctccga tgtgacaatt tcagcagaga ttctctgctt ttctttccca
    12361 attcatgata taataggagt atgattacaa tagttttatt aatcctagcc tatctgctgg
    12421 gttcgattcc atctggtctc tggattggac aagtattctt tcaaatcaat ctacgcgagc
    12481 atggttctgg taacactgga acgaccaaca ccttccgcat tttaggtaag aaagctggta
    12541 tggcaacctt tgtgattgac tttttcaaag gaaccctagc aacgctgctt ccgattattt
    12601 ttcatctaca aggcgtttct cctctcatct ttggactttt ggctgttatc ggccatacct
    12661 tccctatctt tgcaggattt aaaggtggta aggctgtcgc aaccagtgct ggagtgattt
    12721 tcggatttgc gcctatcttc tgtctctacc ttgcgattat cttctttgga gctctctatc
    12781 ttggcagtat gatttcactg tctagtgtca cagcatcgat tgcggctgtt atcggggttc
    12841 tgctctttcc actttttggt tttatcctga gtaactatga ctctctcttc atcgctatta
    12901 tcttagcact tgctagtttg attatcattc gtcataagga caatatagct cgtatcaaaa
    12961 ataaaactga aaatttggtc ccttggggat tgaacctaac ccatcaagat cctaaaaaat
    13021 aaaatgccag ttctgtactg cccccaaaca gttagacaaa taatttatcc aaaggattta
    13081 gttctgtact gcacaggact aagtcctttt agttttacct taattcgttt gttgttgtag
    13141 taatcaatat agtctataat ggcttgttcc aattgattaa gtgatttaaa tgttttctca
    13201 tagccataaa acatttcgga ttttaaaatg ccaaagaaag attccatcct accgttgtct
    13261 tggctgttgc ccttacgtga catggatgct tgaattccct tactctctag gaaccgatga
    13321 taagaatcgt gttggtattg ccagccttgg tcactatgga gaatcgtatt ctcgtagtgc
    13381 ttctctgtga atgcctgttc caacattgtt tgtacttgtt ctaagttggg tgaagttgaa
    13441 agattatagg cgataatttc gctattaaag ccatctaaaa ctggtgataa gtaaagcttt
    13501 taagtactta ctggaatggc aaattctgtc acatctgtgt agcactgttc cattgtttta
    13561 gagccttcaa attggccttg aatgagattc tctgccttct tgccaacgtc tcctttatga
    13621 gaagaatatt ttcgtttctg tcgcgtttta gcttgtaaat tgagtacttt catcaagcct
    13681 tgaactcttt tatgatttac cagataacca cgatttctta gttctaaata aatccgacga
    13741 taagcataat ttcctttgtg ttcgataaaa atggattgaa tttcagcttt aagctcttgg
    13801 tccttatctg gtttatctag ctgtttcaag tgatagtagt aggtcgaacg agctagttta
    13861 atgactttta aaagaagatc taacgaaaac tcagtcatta attcttaaac aatttctgtc
    13921 tttcttcttt ctctttttcc tccttcaatc ggagttctct taactttttt aggatggcat
    13981 tctccgctct caggtactct ccctcttggt ttctcaacaa tagtataccc gtttttcctg
    14041 tattgtgcta gccagttaag aagtatcgta cgacttggga gaccgtattc aagagaaact
    14101 ctatctttag tccagccttc atgtcagact ttattaatca tttctt
//
LOCUS       DAVKNL010000049        13668 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVKNL010000049 DAVKNL010000000
VERSION     DAVKNL010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13668
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.049"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            473..1594
                     /gene="mnmA"
                     /locus_tag="VW411_001558"
     CDS             473..1594
                     /gene="mnmA"
                     /locus_tag="VW411_001558"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEU9794266.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAITLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRRLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGEKTEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            1735..2190
                     /locus_tag="VW411_001559"
     CDS             1735..2190
                     /locus_tag="VW411_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9794267.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYTIGGA
                     IQVNESTEDAVVREVKEELGVKAQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLQNIQLVPVFLKTALPDWEGQLRHIHLEE"
     gene            2200..4113
                     /gene="mnmG"
                     /locus_tag="VW411_001560"
     CDS             2200..4113
                     /gene="mnmG"
                     /locus_tag="VW411_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEU9794268.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEVPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            complement(4320..4412)
                     /locus_tag="VW411_001561"
                     /pseudo
     CDS             complement(4320..4412)
                     /locus_tag="VW411_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(4466..6145)
                     /locus_tag="VW411_001562"
     CDS             complement(4466..6145)
                     /locus_tag="VW411_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU9794269.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(6147..6380)
                     /locus_tag="VW411_001563"
     CDS             complement(6147..6380)
                     /locus_tag="VW411_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002947111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEU9794270.1"
                     /translation="MIYKVFYQETKERSPRRETTRTLYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            6660..6875
                     /locus_tag="VW411_001564"
     CDS             6660..6875
                     /locus_tag="VW411_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794271.1"
                     /translation="MYNLFAFAIGARLVAFIVFVACVYAGNLLFAKLERRQTKFLWKM
                     TFVTLYSLFLLLVTYVVWFVFYFELNA"
     gene            complement(7198..7350)
                     /gene="cibB"
                     /locus_tag="VW411_001565"
     CDS             complement(7198..7350)
                     /gene="cibB"
                     /locus_tag="VW411_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEU9794272.1"
                     /translation="MMKDLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(7352..7537)
                     /gene="cibA"
                     /locus_tag="VW411_001566"
     CDS             complement(7352..7537)
                     /gene="cibA"
                     /locus_tag="VW411_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEU9794273.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            7715..8398
                     /gene="tsaB"
                     /locus_tag="VW411_001567"
     CDS             7715..8398
                     /gene="tsaB"
                     /locus_tag="VW411_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU9794274.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAHTLNIELVGMSSLLALVPH
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMAEAHLSFEEVLEKVKGTSQVTFVGEVGPF
                     VEQIQKHLPRTDYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            8395..8832
                     /gene="rimI"
                     /locus_tag="VW411_001568"
     CDS             8395..8832
                     /gene="rimI"
                     /locus_tag="VW411_001568"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEU9794275.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RQSNQRAQAFYKKEKMTVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            8822..9832
                     /gene="tsaD"
                     /locus_tag="VW411_001569"
     CDS             8822..9832
                     /gene="tsaD"
                     /locus_tag="VW411_001569"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU9794276.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKILVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(9878..11178)
                     /locus_tag="VW411_001570"
                     /pseudo
     CDS             complement(9878..11178)
                     /locus_tag="VW411_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(11454..11805)
                     /locus_tag="VW411_001571"
                     /pseudo
     CDS             complement(11454..11805)
                     /locus_tag="VW411_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(11912..12205)
                     /locus_tag="VW411_001572"
                     /pseudo
     CDS             complement(11912..12205)
                     /locus_tag="VW411_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            12448..13653
                     /locus_tag="VW411_001573"
                     /pseudo
     CDS             12448..13653
                     /locus_tag="VW411_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
BASE COUNT         3958 a         2606 c         3144 g         3960 t
ORIGIN      
        1 ggcttaggtg cccttgcagt caacacaact gtagatggct ataaagtcaa tgccaatggt
       61 gaatgggttt aagccgatta aattaaatca tgttaagaac atttgacatt ttaattttga
      121 aacaaagata aggttcgatt gaatagattt atgttcgtat tctttaggta cctatcttat
      181 gatttcagga aatgtcatta aaaaaacgac tcattttctc taacttgaaa aatagattag
      241 agaaaatggg ttgttttatt tattatagtt atttgaatga agataagaag aaggtatact
      301 cacatcattc acataatctg tatattgact ataagtttta aaaaacaatt tttaagctct
      361 tccttgtctt ctctaaccaa gcgtgttata atgaatactg ctcaagcgac cttcaatcgt
      421 gaagcacaca cgaccttcaa tcgtgaataa acgaatagat gggagactta ccatgagtga
      481 taactctaaa acacgtgttg tcgtggggat gagtggtggt gttgattcgt cggtgacggc
      541 tcttttgctc aaggagcagg gctacgatgt gatcggtatc ttcatgaaga actgggatga
      601 cacagatgaa aacggcgtct gtacggcgac cgaagattac aaggatgtgg ttgcggtggc
      661 agaccagatt ggcattccct actactctgt caattttgaa aaagagtact gggaccgcgt
      721 ttttgagtat ttcctagcgg aataccgtgc agggcgcacg ccaaatccgg acgttatgtg
      781 caacaaggaa atcaagttca aggccttttt ggactatgcc ataaccttgg gggcagacta
      841 tgtagcgact gggcattatg ctcgagtggc gcgtgatgag gatggtaccg ttcacatgct
      901 tcgtggcgtg gacaatggca aggatcagac ctatttcctc agccaacttt cgcaagaaca
      961 acttcaaaaa accatgttcc cactaggaca tttggaaaag cctgaagtac gcagactagc
     1021 agaagaagca ggcctttcga ctgctaagaa gaaagactcg acagggattt gctttatcgg
     1081 agaaaagaac tttaaaaact ttctcagcaa ctacctgcca gctcagcctg gtcgcatgat
     1141 gactgtggat ggtcgcgata tgggcgagca tgcaggtctt atgtactata caatcggtca
     1201 gcgtggcgga ctcggtatcg gtgggcaaca cggcggtgac aatgcccctt ggttcgttgt
     1261 cggaaaagat ctaagcaaga atattctcta tgtaggacaa ggattctacc atgattcgct
     1321 catgtcaact agcctagaag ccagtcaagt ccactttact cgtgaaatgc cagaagagtt
     1381 tacgctagaa tgtacggcta aattccgtta ccgtcagcct gactctaagg tgaccgttca
     1441 tgtcaaagga gaaaagacag aggtcatctt tgcggaacca caacgcgcga ttacaccagg
     1501 acaggcagtt gtcttttacg atggcgaaga gtgtctaggt ggcggtttga ttgacaatgc
     1561 ttaccgcgat ggacaagttt gtcagtacat ttagattgac aaattttctc aatttgctac
     1621 aataataaca gcaatagaaa tgatggtcaa agctcatgga tgttgcaggc ttttttgtcc
     1681 tgcacttctt tgtagttttg actgtttttg tgtcgtttaa gggaaaggac aagaatgact
     1741 caacaagact ttcggacaaa agtagacaat acggtttttg gtgttcgggc tacagccttg
     1801 attgtccaaa atcacaagct tctagttacc aaagacaagg gcaagtatta cactattgga
     1861 ggtgcgattc aagtcaatga aagcacggaa gacgcggtag tccgtgaagt gaaggaagaa
     1921 ctgggtgtca aagctcaagc tgggcagcta gcttttgtgg ttgaaaatcg ttttgaagtg
     1981 gacggcgttt cctatcacaa tatcgagttt cattatctgg tggatttgct tgaagatgcc
     2041 ccgttgacca tgcaggaaga tgagaaaagg cagccctgtg agtggattga cttggataag
     2101 ctccagaata tccagctagt tccagtcttt ttaaaaacag ccctaccaga ttgggaaggc
     2161 caactaagac acattcatct tgaggaatag gagagaaaca tgacttatca ttttactgaa
     2221 gaatacgata ttattgtaat tggtgcggga cacgctgggg ttgaggcttc cttggccgct
     2281 agccgtatgg gctgtaaggt cctgcttgcg accatcaata ttgaaatgct ggctttcatg
     2341 ccttgtaatc cctctatcgg tggttctgcc aaggggattg tcgtgcgtga agtcgatgcc
     2401 ctcggtggcg agatggccaa aaccattgac aagacttaca tccagatgaa gatgctaaac
     2461 acagggaagg ggccagctgt ccgtgccctt cgtgcgcagg ctgacaagga actttactct
     2521 aaggagatgc gcaagacggt tgaaaaccaa gaaaatctga cccttcgtca aaccatgatt
     2581 gatgagattt tggtggaaga tggcaaggtt gtcggtgtgc gtacagccac ccatcaagaa
     2641 tatgctgcta aggctgttat tgtgacgaca gggactgctc tccgtgggga aattatcatc
     2701 ggagacctca agtactcatc aggtcctaac cacagcttgg cttctattaa cctagctgac
     2761 aatctcaagg aactgggtct cgaaatcggt cgtttcaaga caggaacccc tccacgtgtc
     2821 aaggcttctt ctatcaatta cgatgtgaca gaaattcagc caggagacga agtgcctaat
     2881 catttctcat acacttcacg tgatgaggat tatgtcaagg accaagtacc atgctggttg
     2941 acctatacca atggtaccag tcatgagatt atccaaaaca acctccaccg tgcgcctatg
     3001 tttacaggtg tggtcaaggg agtggggcct cgttactgtc cgtcgattga agacaagatt
     3061 gtgcgctttg cggacaagga acgtcaccaa ctcttccttg agccagaagg gcgcaatact
     3121 gaggaagtct atgtgcaagg actttcaacc agtctgcctg aggatgtcca gcgtgacttg
     3181 gtgcattcca tcaaaggttt ggaaaatgca gagatgatgc ggacaggtta tgctattgag
     3241 tatgatatgg tcttgcctca tcagttgcgt gcgactttgg aaaccaagaa aatctcaggt
     3301 ctcttcactg ctggtcagac aaatggaaca tcaggttacg aagaggcagc aggccaaggg
     3361 attatcgcgg gtatcaatgc ggctctgaaa atccaaggca agcctgaatt gattttgaag
     3421 cgcagtgatg gttatatcgg ggtgatgatc gacgacttgg tgaccaaggg aaccattgaa
     3481 ccctaccgtc tcttgaccag tcgtgctgaa taccgtctca ttcttcgtca tgacaatgct
     3541 gatatgcgct tgactgagat gggacgcgag attggccttg tggacgatga acgctgggct
     3601 cgttttgaaa tcaagaaaaa tcaatttgat aatgagatga agcgcctaga cagtatcaaa
     3661 ctcaagccag tcaaggaaac caatgccaag gttgaggaga tgggcttcaa acccttgacc
     3721 gatgcagtga cagccaagga attccttcgc cgtccagaag tttcttacca agatgtggtg
     3781 gccttcatcg gaccagctgc agaagacttg gatgacaaga ttatcgaatt gattgaaaca
     3841 gaaatcaagt atgaaggcta tatttccaaa gccatggacc aggttgccaa gatgaaacgc
     3901 atggaagaaa aacgcattcc ggccaatatc gactgggatg acattgattc tatcgcaacc
     3961 gaagcccgtc agaagttcaa actcatcaat ccagaaacca tcggccaagc cagccgtatt
     4021 tcgggagtaa acccagcaga tatttctatt ttgatggtgt atctggaagg taaaaatcgt
     4081 agtatttcta aaactcttca aaaatcaaaa tgatacgtcg tcggcttctt acgaatgagt
     4141 tcaaagcttg gctttgattc atctccagcc tcccatagtt tcccgaacta tgggagctaa
     4201 ctcaagttat gctttcgctt agccttctag tctgatttcg atttttattg atactcttcg
     4261 aaaatctctt caaaccgcgt caacgtcgcc ttgccgtagg tatggctact gacttcgtca
     4321 gttctatcta caacctcaaa gcagtgcttt gagcaaccta cggctagctt cctagtttgc
     4381 tctttgattt tcattgagta tgagttttaa tctcaaaaga aagcatagag aaaaacagct
     4441 ccgaggacgg ggctgttttc ttgctttatt cttcatctgg tgtgaggatc atcgggatga
     4501 tgatcggttc acgttcggta ttttcataga ggaaggggcg aatagcgttg acaatggcac
     4561 cattgacaga ttgcacgcta gcatccttat ttttcagtgc gatacgaatg gcattgaaga
     4621 ggatacgctg gctttggcga atcaagtcgc cagactctct catgtagaca aagcctcggc
     4681 tgaggatgtc tggaccagat agaatcatct gcgatttgaa gtcaacagtt gcaactgcca
     4741 gaacgacacc gtcttcagat agatcgcgac gatctttgag gacagctgcg ccaatttcac
     4801 cgatacgatt tccatcgaca tagatatctt gggcgttgaa atgacctgcg atacgagctg
     4861 agtcagcagt aagggcaagc acatcgccat tgctcatgat aaagatattg tccttctcaa
     4921 caccagtatc cactgctagt ccagcgtgga ctttttgcat gcggtattca ccgtggacag
     4981 gcatgaagta ttttggctta atcaagcaga gcatgagttt ttgctcttgc tgaccaccgt
     5041 gtccagatgt atggatattg ttcactttac cgtggataac ttcgacacca gcttcagaaa
     5101 tgatgttaat cagcttgttg acactagtag tgtttccagg gatgggacta gaagagaaga
     5161 taacggtatc acctggttgt aattgtactt gacggtgggt tccgttggcg atacgagaga
     5221 gggctgccat aggctcaccc tgactacctg tacagaggat aagaacttct cctgcaggat
     5281 aatctttgat ttcatttggc tcgataaagg ttcccttagg agctttgatg tagccaagat
     5341 cgattccgtt gacaatggcc ttttccatag aacgaccaaa gaccgcaatc ttgcgtccag
     5401 tcttaacagc agcttctgtt gcctgctgga gacggaagat atttgaggca aaggatgcaa
     5461 agatgatacg tccttcaata ccttggataa tcttcataat ggactgacca acgacttttt
     5521 cagagttggt aaaggttggt acttccgcat ttgtcgagtc agacaggaga cagagcacgc
     5581 cttcttcacc aagcgcagcc atacgatgca agtccgcagg ttctccaact ggagtaaagt
     5641 cgaacttaaa gtcacccgta cagacgattt tcccttgagg agtatgaatg acaatcccca
     5701 aaggctctgg aatagagtga gtcgttctaa agaaagttgc cttgagattt ttaaaggtca
     5761 actcggtgtt gtggttgatt tcgtaaagtt tggcgttgcg caagaggccg tgttcttcga
     5821 gtttcccacg gatcaaagcc aaggcaagcg gtccagcata aatagggaca tttgcttgct
     5881 tgagtaggaa cggaatccca ccaatgtggt cctcgtgtcc gtgtgtgatt aaaacagcct
     5941 tgacgcggtc gatattgtcc acgatgtaag agtagtcagg aatgacatag tcgataccaa
     6001 gcaagtcatc ttctgggaat ttaatcccag catcgacgat gataatctcg tcttggtatt
     6061 caattccgta agtgtttttc ccgatttctc ctagaccacc gatggcaaaa acgccgactt
     6121 cttcaggttt aagagtgtag gccatattag aactccgtaa tttcgaaggc gccagtttct
     6181 ttttcgtaat cgagcaattt gtcagacaag agttcgatat actcgatatt gtactctggg
     6241 cgattttctt cgacaagttg gcgagcagtg atacggccct caagttctga gctggcatcg
     6301 atgtctaggt aaagcgtgcg tgttgtttca cggcgtgggc tacgttcttt tgtttcttga
     6361 taaaaaactt tgtaaatcat atagttcctt tctatttaca ggtcagctta ttccaaactt
     6421 ttagcagata tttgcttgat ttcattccat tttgtgtcta gattgacctt gattcgttac
     6481 aattatttca attataccac aaaatgaaaa attagtaaaa gacggaaacg agaaagttag
     6541 gggacgattt ctttggtaag cgttttcaaa aataaagaaa atgtattatg ttgataacgg
     6601 aagtgaaatt tattgcttgt cataattcca tatagatttt aagtcaagga ggttgcccta
     6661 tgtataactt atttgctttt gctatcggag ctcgcttggt agcttttatt gtttttgttg
     6721 cttgcgttta tgcggggaat cttctatttg ccaaactgga acgacgacaa accaagttct
     6781 tgtggaaaat gacctttgtg actctctact cgctttttct cctcctcgtg acctatgtcg
     6841 tttggtttgt attttacttt gaattaaatg cttagatgcc ctgcttatgc tggggttttt
     6901 tgtttgggaa tgtagagata gtagatttac gacacgaaaa ttccacccca tctatcaaga
     6961 tgaggtggtc tttgcatttt tctaacatga catgacaatg gaattttcaa agcttattat
     7021 ttttatggga gtcattgttc tgatcaaaaa tagagttgaa gcataaaaat aaaacaatac
     7081 aaacagataa atcgaatggt aaagggatgg tagtaatgtt caaatcattg agaatgctga
     7141 gcaaactgta agagatgaag acaataaaat ttatccttct aaaaatgtgt ttcttatcta
     7201 gcatgactta ccaaactttt tacgaaggtc ttttccaatg gtataacctg ccataaataa
     7261 agcgatacca acaccgacac caaagccacc cttgatttct tgcaattcct tattagaaat
     7321 ttcacgataa ttgttcaaat ctttcatcat attaatctcc tcctaaaaaa taaccagctg
     7381 ccaaaccaga acctataagt gctgttccac ctgcaatagc cttccaagat actgctactc
     7441 caaagataac caagggagcg agaccgccat caatatctga taattcattt tcagataagg
     7501 ataaaaattg attgtcaaga atgtcaaaat ttgtcattga actttcctcc ttttttcttg
     7561 ttcactttta tattcggaaa agaaaaggaa aagtgagcat ttttttaaat tatttttagt
     7621 taagtgaggg cactcttgtc tgggggcaaa gaaaattccc atgtgccagc ttttgtagta
     7681 taatagtaag cagacaaaaa gataggaatg tgttatgaaa gtattagctt ttgatacgtc
     7741 cagcaaggct ctttctctgg ctattttaga ggataagcag gttcttgccg agacgacgat
     7801 taatattaag aaaaatcaca gtattactct tatgcctgcc atcgattttt tgatggcaag
     7861 tttggattgg acacccaagg atttggaccg aatcgtggta gctgaagggc cgggtagcta
     7921 tacaggcttg cgaattgcgg tagcaactgc taagacctta gctcacaccc tgaacatcga
     7981 gttggttggt atgtcgagtc tcttggctct ggtgccccat caacaagaag gtttgtttgt
     8041 ccccttgatg gatgcgcgtc gcaataatgt ttatgcagga ttttatgaaa atgccaaacc
     8101 tgtcatggca gaagcgcacc tatcttttga agaggtgcta gaaaaagtca agggtactag
     8161 tcaggtaacc tttgtcggag aagttggccc ctttgttgag cagattcaaa aacacttgcc
     8221 aaggactgat tacaaagaaa cattgcccaa tgcagctaat ctagctcttt tggcctggga
     8281 caaggaagca gactccttgc atgattttgt gccgaattac ctcaaacgag tcgaggctga
     8341 ggaaaactgg ctcaagaacc ataccgagtc tggcgagtct tacattaaac gcctatgata
     8401 gaaatcaagc gaattcaaca acagcctgac ctagctcaag ccatctacgc tgttatggca
     8461 gctgtttacc tagtcagtcc ttggactctg gagcaaatcc aagcagatct gtcccaagac
     8521 cagacttggt atgcattggc ttatgatggg gcagaagtga ttggatttct agctgtgcag
     8581 gagaatcttt ttgaagcaga agtcctgcaa atcgctgtca aaggagctta tcagggtcag
     8641 gggattgcgt cagccttgtt tgctcaattg ccgacagaca aggaaatttt cctcgaagtc
     8701 agacagtcaa atcaacgagc gcaagcattt tacaagaaag aaaagatgac agttatcgct
     8761 gagcgaaagg cctactacca tgacccagtc gaggacgcca ttatcatgaa gagagaaata
     8821 gatgaaggat agatatattt tagcatttga gacatcctgt gatgagacca gtgtcgccgt
     8881 cttgaaaaac gacgatgagc tcttgtccaa tgtcattgct agtcaaattg agagtcacaa
     8941 acgttttggt ggcgtagtgc ccgaagtagc cagtcgtcac catgtcgagg tcattacagc
     9001 ctgtatcgag gaggcattgg cagaagcagg gattaccgaa gaggacgtga cagctgttgc
     9061 ggttacctac ggaccaggct tggtcggagc cttgctagtt ggtttgtcag ctgccaaggc
     9121 ctttgcttgg gctcacggac ttccactgat tcctgttaat cacatggctg ggcacctcat
     9181 ggcagctcag agtgtggagc ctttggagtt tcccttgcta gccctcttgg tcagcggcgg
     9241 acacacagag ttggtttatg tttcggaggc aggagattat aagattgttg gggaaacccg
     9301 tgatgatgcg gttggtgagg cttatgataa ggtcggccgt gtcatgggct tgacctatcc
     9361 tgcaggtcgt gagattgacg agctggctca tcaggggcag gatatttatg atttcccccg
     9421 tgccatgatt aaggaagata atctggagtt ctccttctca ggtttgaaat ctgcctttat
     9481 caatcttcat cacaatgccg agcaaaaggg agaaagcctg tctacagaag atttgtgtgc
     9541 ttccttccaa gcagcagtta tggacattct catggcaaaa accaagaagg ctttggagaa
     9601 atatcctgtt aaaatcctag ttgtggcagg tggtgtggca gccaataaag gtctcagaga
     9661 acgcctagca gccgaaatca cagatgtcaa ggttatcatc ccccctctgc gactctgcgg
     9721 agacaatgca ggtatgattg cctatgccag cgtcagcgag tggaacaaag aaaacttcgc
     9781 aggctgggac ctcaatgcca aaccaagtct tgcctttgat accatggaat aaagagtggc
     9841 tctttgtcaa gtgtagtggg taggcgaaaa gctgcaatct ggagagtacg aaattcgtac
     9901 tctctttttt gatgttcaaa gtcatcaaaa tccgtttttt aaaattgtta aagttccgga
     9961 aaccaaaggc tttgcgcttg ataatcttaa tcaaattatt ggtcgcttca agtttagcgt
    10021 tggaataagg cagcttaagt gcgttgataa tcttatctct atcccttaaa aaagtccaaa
    10081 agaccgtttg aaaaagcgga tgaaccgttg gcagttcttg ctcaatcaat ccaaagaaat
    10141 ggtcggcatt cttctcttga aaatgaaaga gcaggagttg atagagttcg tagtgaacct
    10201 gtaatccctc agagtaagaa agcagcttat ctcgtacttc ccgatgggtt aagtgcattc
    10261 gaaacgtggg ctgatagaat cgtttctgac agagcttgaa gctatactgg tgtagtaaat
    10321 cagtccagtg gactgattta gcccgagcct agaaacgaaa aagcgagggt tccaaaagcg
    10381 cttgagcgcc cgatactcca aggattttcg gtcaaattgg ttcatgattt ggattcgtac
    10441 tcggttcata gctcggctca gatgttggac aatgtggaag cggtcaagaa caatcttcgc
    10501 ctttggaaag agttgcttag cgagccgata ataagggctg tacatgtcca tggtgatgac
    10561 ctcgaccagc tcccgaacct ctctctgata gcgttggaaa tggtttcgaa tcaccgcatg
    10621 agttcgcccg tctaaaattg cgaggatgga tttggactca aaatcttggg caatgaagct
    10681 cattttgctc tttttgaagc tatactcatc ccaactcata acttttggca acttggtcca
    10741 atcggtttca aacttaaatt ccctcagttt tcggatgacg gtggaagttg agaccgccaa
    10801 tctgtgggca atctcagtca tagactgctt ttcaaggagg agctgagcga ttttttgtct
    10861 caccatgttg gaaatctggc aatttttctt gacaatggat gtttgagaaa cgaccacctt
    10921 aaggcaattc ttgcactgaa agcggcgctt tttgagatgc agtaccgtgg gtaaaccctg
    10981 acagtcgaga agcggaattt tagaggcttt ctggaagtcg tatttgatca tcttcccttg
    11041 acaatgagga caaggaggag cgggggaatc caactttgcc tgaacgacta gatgggtttg
    11101 gtatttcaga acagacaaga ttttgatatt tttgtcttcc aatccgagca aatctgtggt
    11161 attcttaagt tgttccatat gaaccctttc taatgagttg gttagacgct tttcattata
    11221 ggtcatatgg ggcttttttt ctacaagaaa cgaccctata attcctgggg tgggattacc
    11281 cactacagaa attatagagc caaaacattc caaagtcttg tctgactctg gaatgttttc
    11341 tgttcacagt tgttttgtaa ctgcctaata agtaaccgcg ctataacgag gtatctaccg
    11401 agattgtcaa ttctccagta tactgttata aaaaacaaaa ctggaggatt tttttatgtc
    11461 cccccatcgt cccatcgact gttccgtagc tgcccaataa cggagtatag tcggctgaaa
    11521 caagaatagg acaaaagtgc ctcaagaaaa gcatcgtaat ttgacaatat tattctgaga
    11581 tgtttttgat gtaagcccat attttctcaa tgggattata ctcgggtgaa taggtaggaa
    11641 gtggtaacag tctatgccct gctccttaca catgttcatt ctgtgaaacc ttgcattgtc
    11701 cataatgata agggatggtg tatctaaagt aggtagtaag aatttttgga agcaagcttc
    11761 gaaaaagtca ctcgtcatcg tatctttata ggtcatcgga gccatagtat attgaaacaa
    11821 aaaagggcat tagtacttct ataacagtat cgcaatctct ggtgtgcgtt gtctcgatct
    11881 taattcaaat aattatatct tcttccagag agactaactg cctgctcaag gctcgatcat
    11941 attcgagata aaaataggtc tcaacccctg tctcataaat ataaatagga gtcaggtagc
    12001 ttaagtgatt caattcttta aggaaccgat ttactttttc tgggtcttgt tcgcagtagg
    12061 tacggctctt ttttagacta tatcccatag cctttagagc gtaatgaata gttgttggag
    12121 gacagttgaa ttcagcagct atttcagtca aataagcatc gggatgagtt tcaatataga
    12181 tttttaattt atctctatca attttttcaa ttaatagtgg aagttttctt gaagttagtg
    12241 tattgaaacg tttttataca gtgctaaaat ttgaagtgta gtgcagatgg atttgtaata
    12301 gtattatagc aattctttgg agaaggtcga gacaagataa aataggtatc tttgagatat
    12361 aagcatgatt tactataaaa ttattgagtt attcataaaa atttctctat caaatatcat
    12421 aatgcgtcgt ttaaggagat ggttacaatg caatatagtt gtggaaaaat aaatattaat
    12481 atccctgatg gatatggaga cattaaggat atagtgtttt cggctcatat tatagttagg
    12541 tataataatg gacattgtgg aggtatagat ccgcatataa ttggactttg taaaaagcag
    12601 atcagaagga tgtctctata tcctattttg ataattgtat ctagagattc aaaggttatt
    12661 gatgattata aaaatttaga tattgcctat gttgattgta ctcaatgttc aaataatttt
    12721 gaaactgcct tacacgttaa aaatatttta aaattattaa aaatccaact tatacactgt
    12781 catggctact caactaatta ttttttatat atgttaaaaa aacttgacaa gaatggattt
    12841 ggaaaggtta agacagtgat aacatgccat ggatgggttg aatataactt aaaaaagaaa
    12901 ttcttgactt attttgattt ttggacatat tctatgggag atgcttttat ttgtgtatct
    12961 gaaactatga aaaaaaagat tggagagtat aataaaaaat aagaagatag ttgcaataaa
    13021 taacggaatt aatgtatcaa atagcgattt agatgtagta ggtgttcaag actttaaaaa
    13081 agaattttgt attccaaata acaaaaaatc atttgttatg ttggaaggtt ggatccagaa
    13141 aaaaggccgg atagattcct tgaatttgca gaaaaattat ttttagtcag agaagatgtg
    13201 atttttatta tggctggaaa tggtagtatg tgggctgctt tgaaagaaaa aatttgccat
    13261 ttgaaatgta gagataattt caggttactt ggtgaaatct atccagctac tatagtttac
    13321 caaatatcgg atttattgta tattccttct gatacagaag gaatacctat gtgtgtttta
    13381 gagtcaatgt cacaaggaac tccggtttta gctagtaatg ttggcgggtt aagtgaaatt
    13441 attgaacata gggttgatgg atttttattt gagaaggaag atgttgaggg agtgtgtgct
    13501 tgtgctaatt ttttactcaa tgattctgag tatttgaaat atataggtga gaatagtaaa
    13561 tcaaaaataa gaaaacattt ttctgtgcaa aaaatgtttg tagaaaccat gagagtatat
    13621 gatgaattat tagagaagag tagtcatgga taggaatata gatcaaga
//
LOCUS       DAVKNL010000050        12413 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVKNL010000050 DAVKNL010000000
VERSION     DAVKNL010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12413
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.050"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(164..796)
                     /locus_tag="VW411_001574"
     CDS             complement(164..796)
                     /locus_tag="VW411_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9794277.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAVQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKDTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAITWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            complement(818..1690)
                     /gene="sdaAA"
                     /locus_tag="VW411_001575"
     CDS             complement(818..1690)
                     /gene="sdaAA"
                     /locus_tag="VW411_001575"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEU9794278.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            complement(1699..2370)
                     /gene="sdaAB"
                     /locus_tag="VW411_001576"
     CDS             complement(1699..2370)
                     /gene="sdaAB"
                     /locus_tag="VW411_001576"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEU9794279.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            2612..3199
                     /locus_tag="VW411_001577"
     CDS             2612..3199
                     /locus_tag="VW411_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU9794280.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            complement(3251..3571)
                     /locus_tag="VW411_001578"
     CDS             complement(3251..3571)
                     /locus_tag="VW411_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9794281.1"
                     /translation="MQRLEVYKNYQRLYDLRIAILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVPIGICTDDSKLIQKGFSLLELTEETSMLSHLKKEVEIYYQ
                     AKER"
     gene            4002..4289
                     /locus_tag="VW411_001579"
     CDS             4002..4289
                     /locus_tag="VW411_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEU9794282.1"
                     /translation="MRKLVKIGVASLMVCGILATTNALAVWVEGGQWNYGVGWTGTFG
                     YSDYLHSTRYHTATVRHGGRTSKDYAKPEAWARASLTKIPPTGMEYFYGFE"
     gene            4341..6449
                     /locus_tag="VW411_001580"
     CDS             4341..6449
                     /locus_tag="VW411_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9794283.1"
                     /translation="MKKISNFCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDL
                     YLKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEM
                     DKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPML
                     WLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLIFNYVMKIGTTILLTAL
                     LIGVGRSLEQENKELAYQQQWVSQGNYLTLETFKLNDNLWQEELAGSGKSTDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPNTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVTTFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSFPLLVLIFFAHAFLPLLLFAGFTCLDILFVL
                     GLASRMEKRSLVELLKGGIL"
     gene            6446..7087
                     /locus_tag="VW411_001581"
     CDS             6446..7087
                     /locus_tag="VW411_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9794284.1"
                     /translation="MIELKNITKTIGGKVILDNLSLRIDQGDLVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLPKKDKLKKVEEILERVGLSATLHQRVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRIIELGEGK"
     gene            7294..8100
                     /locus_tag="VW411_001582"
     CDS             7294..8100
                     /locus_tag="VW411_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9794285.1"
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVAL
                     NPDFAPFEYQKVVDGKNQIVGSDIELAKAIATKLGVELELSPMSFDNVLASVQSGKAD
                     LAISGVSKTDERSKVFDFSIPYYTAKNKLIVKKSDLTTYQSVNDLAQKKVGAQKGSIQ
                     ETMAKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVSKGFVENNPDLAIADLNF
                     EKEQDDSYAVAMKKDSKKLKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            8066..8536
                     /locus_tag="VW411_001583"
     CDS             8066..8536
                     /locus_tag="VW411_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEU9794286.1"
                     /translation="MRMPLKHPLKNRKKEKNMSKEKVILAYSGGLDTSVAITWLKKDY
                     DVVSVCMDVGEGKDLDFIHDKALKVWAVESYVIDVKDEFATDYVLVAHQSHAYYEQKY
                     LLVSALSRPLISKKLVEIAHQIGATTIAHGCTGKGNDQVEYQIAVAKKANEAKK"
     gene            complement(8723..9043)
                     /locus_tag="VW411_001584"
     CDS             complement(8723..9043)
                     /locus_tag="VW411_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9794287.1"
                     /translation="MQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKKEVETHYQ
                     PKKL"
     gene            9801..10181
                     /locus_tag="VW411_001585"
     CDS             9801..10181
                     /locus_tag="VW411_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0115 family bacteriocin-like peptide"
                     /protein_id="HEU9794288.1"
                     /translation="MELVLPNNYVALEQEEMMYLDGGGVGRNWWNSRGSFATVLDVDL
                     AIYSGGATIYSAYAIKKAISANRGAITRTLRSLIIKHVGSAAGHLVNTALNVALTVTG
                     FSLGGAIAYGADWADGSLDGYIFA"
     gene            10839..11078
                     /locus_tag="VW411_001586"
     CDS             10839..11078
                     /locus_tag="VW411_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794289.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     DLHKITRDYASQTKPAIQDGVFILEDFRNILCTMM"
     gene            11097..11342
                     /locus_tag="VW411_001587"
     CDS             11097..11342
                     /locus_tag="VW411_001587"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794290.1"
                     /translation="MSLSAISSISKLASCCIVAKDLSKNTMKLDVIFFEDKNKRSAVL
                     GLRRDKSGVFKPVTLHFTSAKKYAKVRKTDVKEMKWL"
     gene            11631..>12413
                     /locus_tag="VW411_001588"
     CDS             11631..>12413
                     /locus_tag="VW411_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794291.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSQPTFVRAEESPQVVEKSSLE
                     KKYEEAKAKADTAKKDYETAKKKAEDAQKKYEDDQKRTEEKARKEAEASQKLNDVALV
                     VQNAYKEYREVQNQRSKYKSDAEYQKKLTEVDSKIEKARKEQQDLQNKFNEVRAVVVP
                     EPNALAETKKKAEEAKAEEKVAKRKYDYATLKVALAKKEVEAKELEIEKLQYEISTLE
                     QEVATAQHQVDNLKKLLAGADPDDGTEVIEAKLKKGEAELNAK"
BASE COUNT         3941 a         2018 c         2651 g         3803 t
ORIGIN      
        1 atccacaacc ttaaaacagt gttttgagct gacttcgtca gttccatcta caaccttaaa
       61 acagtgtttt gagctgcccg cagctagttt cctagtttgc tctttgattt tcattgagta
      121 ttacttcatt ttcttctgaa atggaattgt tacccagtct atgctattga aaatacgcca
      181 aaacttctaa gggtttgtga gcgatataat caggttgata gtttagtaga tctgcttgct
      241 ctccaaatcc ccaagtgatg gccaatttct gaatacctgt ttctcgagct cccagcatat
      301 caaacttggt atctccgatg atgatggctt gttctggtgc tagttgatgt gtctgcaagg
      361 cttggtgaat gacatctgcc ttatggggtg cttcagggct agaaccataa atgccatcaa
      421 agaaatgatg gatttccaag ttttttgcca tgtcttgagc agtagatgta tcctttgtcg
      481 tggtgatgta gagtggataa ctgctcgata actcctcaag caagtctata atctgaggaa
      541 agagttgagc ttcatagatg ccttttgcct tatagtaaga acgatatatc tgcacggctt
      601 cagaaatttg gtctttggac aggcaggtcg caaaactact ttcgagaggt ggtcccataa
      661 aaccacgaat agttttggca tcagggctag gcacccccag ctctttaaag gtataggtaa
      721 aggcattgtg aatcccgata gaactatcaa cgagggttcc atccaaatcg aaaaaaatcg
      781 ctgtgataga ggtcatggtt tctcctattt gataagctta ttctccgaaa atttcttttt
      841 ggaggcgacg accagtaggg gtggtagcga gtccaccttc agctgtttca cgaaaggcag
      901 ttggcatgct tgctcctact tggtacatgg catcgatcac ttcatccaca gggattttag
      961 attcgatacc tgccaaggcc atgtctgctg cgatgaaagc aaagctagct cccatggcat
     1021 tacgtttgac acagggaact tcgaccaaac ctgcaacagg gtcacagatg aggcctagca
     1081 tatttttaat gacaaaggca atagcttgac tggcctgata aggtgttcca cctgcagcca
     1141 gagtcaaggc ggcagcactc atagcagagg ctgaaccaac ttcagcttga cacccaccct
     1201 cagcacctga gatggaggca ttgtttgcga tgactagtcc aaaggcacca gcagcaaaga
     1261 ggaaatccaa ttgttgctcg tggctgaggt ctaatttttc aatagcagca gtgagaacgg
     1321 atggcagaca gccagcactt ccagcggttg gagtggcaca gaccaagccc attttggcat
     1381 tgtgttcatt gactgcgatg gcatttcggg cagccgagag aatcgtataa tctgacagag
     1441 tttttccgtt ttcgatgtag tgatccaatt tggcagcatc tccacctgtc aggccactac
     1501 gagatttatt ttcattgagg ccaagttgga cagaggcttt cataacttcc agattgcgtt
     1561 ccatgagaag gaagacttct tcacgttcgc gaccggtcaa ttcaaactct gttgtaatca
     1621 tgagttctgc gacatttcct tgaaagtcca gatctgcttg ctcgaccaat tctttgatag
     1681 aataaaacat gcttcctcct atttaaagaa attgacattg tggagatgag ggatttttcg
     1741 aatttcttcg atagcctcat cacagttgcg actgtcaact tcgataatca taatggcttt
     1801 ttcaccagct ttttcacgag tgacattcat ctgggcgata ttgataccat agcgggaaag
     1861 cgcctctgta acaagggcaa tcatacctgg aatatcttga tgaacgatga tgatagtcgg
     1921 tgtattcata ttgagagaga cggcaaaacc attgagttcg gttacctgaa tatttcctcc
     1981 accgatagaa ataccagtca cgctgatggt cttgtgggca tttttaacag taattttagt
     2041 ggtgttaggg tgaggggcat tgctgtcttt ctgaatggtc cagacaatct tgataccacg
     2101 cttgtgggca atttccagac tatttggaat ttcaggatca tctgtatcca ttcctaaaat
     2161 acctgcaaca agggctaggt ctgttccgtg accacgatag gtcttggcaa atgagttaaa
     2221 aagttggaat tcaacttctg tcggagtatc atcaaaaatg gaagagacaa tcttcccaat
     2281 acgaacagca ccagcggtat ggctactaga tgggccaatc ataactggtc cgatgatatc
     2341 aaagacagat tgaaaacgaa gtgatttcat cagtttcccc ttataaaaat tcttatctct
     2401 attatatcaa agaatgaggg gcttggcttt aattgtggat gaaaaccttt ctaatacctc
     2461 aaatagcata aaaatagtat cttttatgac aaaaaacacc ttatttaggg aaataaaaaa
     2521 taattttgta atatttctac ataaaagtgt caagaaacgg taatatttaa agggtatgat
     2581 agaactatag aaagaaggag aattttcgaa tatgaaatca ataactaaaa agattaaagc
     2641 aactcttgca ggagtagctg ccttgtttgc agtatttgct ccatcatttg tatctgctca
     2701 agaatcatca acttacactg ttaaagaagg tgatacactt tcagaaatcg ctgaaactca
     2761 caacacaaca gttgaaaaat tggcagaaaa caaccacatt gataacattc atttgattta
     2821 tgttgatcaa gagttggtta tcgatggccc tgtagcgcct gttgcaacac cagcgccagc
     2881 tacttatgcg gcaccagccg ctcaagatga aactgtttca gctccagtag cagaaactcc
     2941 agtagtaagt gaaacagttg tttcaactgt aagcggatct gaagcagaag ccaaagaatg
     3001 gatcgctcaa aaagaatcag gtggtagcta tacagctaca aatggacgtt atatcggacg
     3061 ttaccaatta acagattcat acctgaacgg tgactactca gctgaaaacc aagaacgtgt
     3121 agcagatgcc tacgttgcag gacgttacgg ttcatggact gctgctaaaa acttctggct
     3181 taacaatggc tggtattaag aactaacgaa caaataatat gtaaagagtc gaggaaatcc
     3241 ctcggctttt ttatctttcc ttcgcttggt aatagatctc tacttctttt ttgagatgag
     3301 acagcataga agtttcctcg gttagctcca gaagggagaa ccctttttgg ataagtttag
     3361 aatcgtctgt acaaatccca atagggacat agcagatagc cagcctatcg taacttttct
     3421 tattcttggc gtcctccagt aaagtatagc agatttgctt acacatgttt ttatcttgat
     3481 tgtatagata gagtgtggaa aggttgagca ggattgctat tcgcaagtca tataaacgtt
     3541 gatagttttt atagacttct aagcgttgca agattttctc ggattattga caaataaacg
     3601 gacattctga tagacgattg ccctagcggc ttagtgtaaa ctttacaaat ttggcttgta
     3661 atttttttat aaaattcact tcagatactt gacagttatt atattttcgc ttatgcttat
     3721 tatatagata aaaggacggg ataggagttt aaaaatttga aagcgctatc catattgttt
     3781 attggactga ttcttgagta atagttagag tttgcttaat actactctgg ttgttcaagg
     3841 acctgataca gaagcgcacc attctcccgg ttaagagtaa gttcgttgtt aaaaaatgaa
     3901 atgagataga aggaaggtga gtccaatgga ctacgatact tgttgataaa ttactatagc
     3961 tgtttttacg gaacagctct cgataatagg agaaataaat catgagaaaa ttagttaaaa
     4021 ttggtgttgc atctttaatg gtatgtggta tacttgctac tacaaatgct ttagcagtat
     4081 gggttgaggg tggtcaatgg aactatggag taggttggac aggaactttt ggatattctg
     4141 attacttaca ttctactcga tatcatacag caactgttag acatgggggt agaacctcta
     4201 aggattatgc aaaacctgag gcatgggcta gagcttccct caccaagatt ccgccaacag
     4261 gaatggaata tttctatgga tttgagtaaa attcttccct ctcctatttg gagagggaaa
     4321 actgtagtgg aggcactttt atgaaaaaaa tcagtaattt ctgtatgtta ctcctgcttc
     4381 tgtgtaccac tttttttgtt tttaatgtaa actatacacg agaagtggtt cggattcaag
     4441 aaatgggaaa gactgtagat tctttggatt tgtatttgaa agatattaac gaacctgcag
     4501 cgtctgttct tcgatttttt gaggatgtat caaaggagta taaagtctcc atcatcaaaa
     4561 cagacagtgg tgatgaggtg gtcaagtctg gtgtttttga taaagatacc ttcccctacc
     4621 aagagtttgg gatttcttct cttgatttta ccacagatgg tgaaggagtc tatagtaata
     4681 aagaaatttc caataaactt ggtacgattc cgacctttct aaaagccaaa cctattcagc
     4741 ttatgacttt tcaaacctat atcaaggata catctcgtag tttaaatggt cgctatacga
     4801 taacttctac acaagagatg gacaaggata ggattgtaca gaaatggagc gattttttca
     4861 agatagacca ggctaccttg ctagagccga cctacaaaag tgcagtggaa gtcataaatc
     4921 gagatttgct tttatctgcc attgtttttg tcttggctat tttgcttctt gtgttagtga
     4981 cagtgtatca accgatgatg gagatgaaaa gagttggggt acaaaaatta cttggttttc
     5041 aagatagggc tgttttagct gatgttgtaa aaggcaacct ttacctcctc ctaggtgggg
     5101 ctcttgtgat caatctaggc gtgtttttct tgcttgatta taagccaaaa gatttgtttc
     5161 ctatgctgtg gttgtctcat tttttgctgt tgcagcttta tctctttatc agttggttga
     5221 cttacctctt aatccaaaaa atgacaatca gctctctgct gaaaggtttt tcatctttca
     5281 aatttggtct tatcttcaat tatgtgatga aaatagggac aactatttta ctgacggcct
     5341 tactgattgg ggtgggcaga agtttagaac aagaaaacaa agaacttgct tatcagcaac
     5401 agtgggtaag tcaaggtaat tacctgacct tagaaacctt caaactcaat gataatctgt
     5461 ggcaagaaga gctagcaggg tcagggaaat ctacagatta tttctatcga ttttatcagg
     5521 atttggtaga aaaaacgcag gcgggctatg tgcagagtag cagtcttcct gtaaaaaatt
     5581 ttgtccaatc agaacagatt cagcaatatc agttaacaga tacggtggat gtttactatg
     5641 ccaatcgcaa ttttctaaag agcaagggat tcaagctacc aaataccggt attaaaaaag
     5701 ttattttgat gccagcaagt acgaaaggtg aagaagataa aaatcagctc ttggggaagt
     5761 taattgcctt tcattcgatg aagtatgaag agcagcaaaa acgaacgata gaggagatgg
     5821 atgtcgagat tgcctattat gaaggagatt ggtcattttt cccatatagt gataagcgaa
     5881 aggaaaatct ctccaatcca attattagct tggtcaatga ttctgatatg atgtgggatg
     5941 agaaagcctc cctgtcaaca actggcttaa ataatccgat taaaattgaa aatacggttc
     6001 aacatcaaaa agagattaca gagttagttg agaaattgtc agatggaaat tatttaaaat
     6061 tttcatctat tcaagccatt caacaagaga aagtggattc ttatcgagat gctgttcgga
     6121 attttaacct actctttgct ttgtttggtc tccttagcat gatgatttcc tacttcttac
     6181 tagtaacaac tttcttattg aagcgcaggg atatcattac caagaagttt atggggtgga
     6241 aactggtcga tcgctaccgt cctctcctcg ttctgctctt gctgggctat agtttccctc
     6301 ttctagtctt gattttcttt gcccatgcgt tcttaccact tctactgttt gcaggtttta
     6361 catgtctgga tatactattt gtgctaggct tagcttctag gatggagaaa agaagtctag
     6421 tagagttatt gaaagggggc atcttatgat tgagttgaaa aatattacca aaaccattgg
     6481 ggggaaagtg attttggata acttatctct caggattgat cagggggatt tggtagctat
     6541 tgttggtaag agtggtagtg ggaagtcgac cttgttaaat ttattgggtt tgatagatgg
     6601 tgattatagc ggacggtatg agatttttgg tcagacaaat ctagcggtta attctgctaa
     6661 gtcgcaaaca ataatccgtg aacatatctc ttatctgttt caaaattttg ccctgattga
     6721 tgatgaaacg gtcgagtaca atctcatgct ggcgctgaaa tatgtgaaat tgcctaagaa
     6781 agacaagctc aaaaaggtgg aagagatttt agagagagta ggtttatcag ctactttgca
     6841 tcaaagggtc tccgagttgt ctgggggcga acaacaacga attgcagttg ctagagccat
     6901 cttaaaaccc agccagctga ttttagccga tgaaccgaca ggttctctgg atcctgaaaa
     6961 tagagatttg gtcttgaagt ttctcttaga gatgaatcga gaggggaaaa cagtcattat
     7021 cgtgacccac gatgcttatg tagcccaaca atgtcatcga atcattgaat taggcgaggg
     7081 aaaatgagtt cgttcagctt catttgaccg actgaatact catatttttc agagagaaat
     7141 agcataaata ggcctaggaa tgatattttt atgtcatttt cctgggtttt tctgttttaa
     7201 attgaaaaaa attacaattt aggcttgaca aaggatgcat atagatgtat tgtttataca
     7261 aataaaagga ataaacataa agtaggcttt attatgaata agatgaagaa ggtgttgatg
     7321 acgatgtttg gtttagtgat gctcccccta ctatttgctt gtagcaacaa tcaatcggct
     7381 ggaattgaag ccatcaagtc caaaggaaaa ttggttgtag ccctcaatcc ggattttgct
     7441 ccatttgaat accaaaaagt ggttgatggg aaaaatcaga ttgtgggttc agatatcgaa
     7501 ttagccaagg ctatcgcaac aaaactaggt gtcgaattgg aactatctcc catgagtttt
     7561 gataatgtac tggctagtgt tcaatcagga aaagccgacc ttgccatatc aggtgtttct
     7621 aagacagatg aacggagcaa ggtgtttgac ttttccattc cctactatac tgcaaaaaat
     7681 aaactcattg tcaaaaaatc tgacttgact acttatcagt ctgtaaacga cttggcgcag
     7741 aaaaaggttg gagcgcagaa aggttcgatt caagagacga tggcgaaaga tttgctacaa
     7801 aattcttccc tcgtatctct gcctaaaaat gggaatttaa tcacagattt aaaatcagga
     7861 caagtggatg ccgttatctt tgaagaacct gtttccaagg gatttgtgga aaataatcct
     7921 gatttagcaa tcgcagacct caattttgaa aaagagcaag atgattccta cgcggtagcc
     7981 atgaaaaaag atagcaagaa attgaaagag gcagtcgata aaaccattca aaagttgaag
     8041 gagtctgggg aattagacaa actcattgag gatgccttta aagcatccat tgaaaaatag
     8101 aaagaaggaa aagaacatga gtaaagaaaa agtaattttg gcctattctg gcggtttgga
     8161 cacatcagtt gctattacct ggttaaagaa agactatgat gttgtttcag tttgtatgga
     8221 tgtgggtgaa gggaaagact tagatttcat ccatgataag gctcttaagg tttgggcagt
     8281 agaatcttat gtcattgatg ttaaggacga atttgctaca gattatgtgc tagtggctca
     8341 tcagtcacat gcctattatg aacaaaagta tctcttggta tctgccttga gccgccctct
     8401 gatttctaaa aaattggttg aaatagcgca tcagatagga gcgactacaa ttgctcatgg
     8461 ttgtaccggt aaggggaatg accaagtcga ataccagatt gcagtcgcta agaaagcgaa
     8521 tgaagctaaa aagtgatgga gtgatgggtt cagaataaga ggttggtaga ttatcagcct
     8581 ctttcttgtc gttgaaaaag tgagatatat tgacttttga aaaaaatgtc ataattgagt
     8641 tgggataaac gtaaaagtca tactttctcg ttaaatcgtt cggcgattct atgaaaaggt
     8701 cgaggagctc cctcgacttt ttttataatt tctttggttg ataatgggtc tctacttctt
     8761 ttttgagatg agacagcata gaagtttcct cggtcagctc cagaagggag aacccttttt
     8821 ggataagttt agaatcgtct gtacaaatcc caatacggac atagcagata gcaagcctat
     8881 cgtagctttt cttattcttg gcgtcctcca gtaaagtgta gcagatttgc ttacacatgt
     8941 ttttatcttg attgtataga tagagtgtgg aaaggtttag caggattgct attcgcaagt
     9001 catataaatg ttgatagttt ttatagactt ctaagcgttg caagattttc ttggattatt
     9061 gacaaataaa cggatattct gatagacgat tgccctagcg gcttagtgta aactttacaa
     9121 atttggcttg taattttttt ataaaattca cttcagttac ttgacagtta ttatattttc
     9181 acttattata tagataaaag gatgggatag gagtttaaaa atttgaaagc gctatccata
     9241 atcataacgc ataattttct ccttttcgtg aaaaaagtta acgtaatttt gaaatgagct
     9301 taataagagg tatgatgtag gtagaaaata aagataggag aaaaagtatg aataaaaaaa
     9361 tattttgtat tctagtatgt attatatcat tgattagtct agctataatc tttccatggg
     9421 gctggtcgat ataatgtaag ggagcttgag ttgttagtgg aggataaaat gttaagagta
     9481 atagtagtta ttcaatcttt agtaataata ctgcttgctg ttgctttatt attatctcgg
     9541 aaaaactttg attcgttaaa tgttgttctt ataatgatga tcgtatcaca attgttgtta
     9601 ttagtatatt tgataaaatc agggaatcag tgattagctg ttatttgaat taagtaaact
     9661 gttaagcttc aagcagtaac tggttttaaa tatttaaaat aagataaagg aaggaagtct
     9721 aatttaggta actaaacaaa gcctgcgatc acttgttagg aaaccatcct aaccatgctt
     9781 aataaaaaag gagaaaataa atggaactcg tattaccaaa taattatgtt gctcttgagc
     9841 aagaagagat gatgtatctt gatgggggtg gtgttggtcg taactggtgg aatagtagag
     9901 gtagttttgc aacagttctg gatgtagatt tggccatcta tagtggtggt gcaacaattt
     9961 attctgctta tgcgataaaa aaagctatct cagctaatag aggggctatt acgagaacat
    10021 tacgtagttt aataattaaa catgtaggta gtgcagctgg ccatttagtc aatactgcac
    10081 taaacgttgc actaactgtt actggatttt cactaggtgg agcaatcgca tatggggctg
    10141 attgggctga cggtagctta gatggttata tttttgctta aaatgaaaca tttttttgca
    10201 ggaattggtg aaataaactt tgtaagttat ttattgtata tatgtgtagg aatagcccct
    10261 ttttttcacg tatatataat aggttctgaa atgaattttg tgaaaatagt gttatctatt
    10321 ttgggagtta tatttgtaag tatgttaact attgctcgaa tttatcggac ttttctataa
    10381 agaatagttt ggacttgaac tgtatctaaa aacagaggtt ggtagattat cagcctcttt
    10441 cttgtcgttg aaaagagtga gatatattga cttttgaaaa aaatgtcata attgagttgg
    10501 gataaaccta aaagtaataa tatagtagat tgaatctgga atagtccgtt atgactgtta
    10561 aaatatttct agaaattaat ttgactttcc taatcgattt gttcacatct tgtttcaatc
    10621 tactatactt tctcgttaaa ccgttcggta attcatggtg acatggacac atagtagtaa
    10681 gcacactatg tggcttggca gatctaaaaa ctgttccctt gcgataagcc taataagcac
    10741 aacataggga gttagagaag ccgactctaa tcaaccactt tgggcagtag tggtaactgc
    10801 cccgtgcttt ttattttgag aaaatatgga gtttgtcgtt gaaattactt gattgtattt
    10861 tggattatca agaaagattc aatggaaaaa catgtcaagt atcaacgaat tataagtatt
    10921 tagagatttt caaagtgaat ttttgcttga ctgatttaca tcatttattt gacttacaca
    10981 aaatcacacg agattatgct agtcaaacaa aacctgctat tcaagatggt gtttttattt
    11041 tagaagattt tagaaatatc ctatgtacaa tgatgtaatc gaaaggatat ctttatgtga
    11101 gtttatcggc gatatcttct atatctaagc tagcaagttg ttgcatcgta gctaaggatt
    11161 tatctaaaaa tactatgaaa ttggacgtca tattttttga ggataaaaat aaaagatccg
    11221 cagttttagg tttacgaaga gacaaaagcg gagtatttaa accagttact ctacatttta
    11281 caagcgctaa gaaatatgct aaagttcgta aaacagatgt gaaagaaatg aaatggctat
    11341 aaagattgtc cgcaggctta agcttgcgtt ttttgttgtt taacgaaatt aaaaaacaag
    11401 ctctctcatc ggaaatgttt ttataggaat gaaggaagat gatgctatgt ttaaaaagag
    11461 aatcgaaaag ggcaaaagta gtatctttat ctattttttg gagtagaagc ttatgatata
    11521 gaaatttgta ataaaaatgt aatataaaac atttgacaaa tatttacggg gggagtatac
    11581 ttaatataat tatagtctaa aaatgactat cagaaaagag gtaaatttag atgaataaga
    11641 aaaaaatgat tttaacaagt ctagccagcg tcgctatctt aggggctggt tttgttacgt
    11701 ctcagcctac ttttgtaaga gcagaagaat ctccacaagt tgtcgaaaaa tcttcattag
    11761 agaagaaata tgaggaagca aaagcaaaag ctgatactgc caagaaagat tacgaaacgg
    11821 ctaaaaagaa agcagaagac gctcagaaaa agtatgaaga tgatcagaag agaactgagg
    11881 agaaagctcg aaaagaagca gaagcatctc aaaaattgaa tgatgtggcg cttgttgttc
    11941 aaaatgcata taaagagtac cgagaagttc aaaatcaacg tagtaaatat aaatctgacg
    12001 ctgaatatca gaaaaaatta acagaggtcg actctaaaat agagaaggct aggaaagagc
    12061 aacaggactt gcaaaataaa tttaatgaag taagagcagt tgtagttcct gaaccaaatg
    12121 cgttggctga gactaagaaa aaagcagaag aagctaaagc agaagaaaaa gtagctaaga
    12181 gaaaatatga ttatgcaact ctaaaggtag cactagcgaa gaaagaagta gaggctaagg
    12241 aacttgaaat tgaaaaactt caatatgaaa tttctacttt ggaacaagaa gttgctactg
    12301 ctcaacatca agtagataat ttgaaaaaac ttcttgctgg tgcggatcct gatgatggca
    12361 cagaagttat agaagctaaa ttaaaaaaag gagaagctga gctaaacgct aaa
//
LOCUS       DAVKNL010000051        12408 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVKNL010000051 DAVKNL010000000
VERSION     DAVKNL010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12408
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.051"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            164..973
                     /locus_tag="VW411_001589"
     CDS             164..973
                     /locus_tag="VW411_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEU9794292.1"
                     /translation="MLLQKELIPMIEANLPNMAYAEKDIAKFFLKQQPLNDYSSKALC
                     EYLNVSKATLTRFAKKCGFKGFRQFIFKYQEMIHEKEKLALYTEATEKVLSDYEEMLR
                     KTYTVLDEVQLERIAEMIETAERVYLYGKGSSVLALQEMKMRFMRLGVIGEVLSDEDM
                     ILWSSLLLNENCLVIGASISGQTDIVLEGLQKAADKGAKTVLMTTRKFDEEDCFFDEL
                     LLLASTDHLSYGNRISPQFPILLITDCLFSNYLESPERQYYYNQTIIHKEE"
     gene            978..1679
                     /locus_tag="VW411_001590"
     CDS             978..1679
                     /locus_tag="VW411_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEU9794293.1"
                     /translation="MSQIWTKEKFISQVQGGVIVSCQALPGEALYNEEFSLMPFMAKA
                     ALEAGAVGIRANSVRDIKAIQKVVDLPIIGIIKRDYPPQEPYITATMKEVDELVECGT
                     TVIAFDATLRPRYDGLVVSEFIKKIKEKYPNQLLMADVSNLDEGLYAFKSGVDFVGTT
                     LSGYTSTSVQSDEPDFELMKKLADFNIPVIAEGKIHYPEQLKKAYSLGVTSVVIGGAI
                     TRPKEIAQRFINVIK"
     gene            1704..2621
                     /locus_tag="VW411_001591"
     CDS             1704..2621
                     /locus_tag="VW411_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HEU9794294.1"
                     /translation="MKDLTKYKGVIPAFYACYDENGEISQDRVKSLVQYFIDKGVKGI
                     YVNGSSGECIYQSVEDRKQIIEAVMEVAKGKLTVINHIACNNTKDSIELAKHSESVGV
                     DAIAAIPPIYFKLPEYSIAAYWNAMSEAASNTDFIIYNIPQLAGVALTGSLYATMRQN
                     PRVIGVKNSSMPVQDIQMFVAAGGEDYIVFNGPDEQYLGGRLMGAEAGIGGTYGVMPD
                     LFLKLESLIQERDLDTAKKLQYAINEVIYKMISGKANMYAVAKEVLRLNEKLDLGSVR
                     QPLEALAEGDLEVAKQAAELIQQARKEFL"
     gene            2649..4190
                     /locus_tag="VW411_001592"
     CDS             2649..4190
                     /locus_tag="VW411_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEU9794295.1"
                     /translation="MGTTGFTIIDLIILIVYLLAVLVAGIYFSKKEMKGKEFFKGDGS
                     VPWYVTSVSIFATMLSPISFLGLAGSSYAGSWILWFAQLGMVVAIPLTIRFILPIFAR
                     IDIDTAYDYLDKRFNSKALRIISALLFIIYQLGRMSIIMYLPSAGLSVLTGIDINILI
                     ILMGVVAIVYSYTGGLKSVLWTDFIQGVILISGVVLALFVLIANIKGGFGAVAETLAN
                     GKFLAANEKLFDPNLLSNSIFLIVMGSGFTILSSYASSQDLVQRFTTTQNIKKLNKML
                     FTNGVLSLATATVFYLIGTGLYVFYQVQNADSAASNIPQDQIFMYFIAYQLPVGITGL
                     ILAAIYAASQSTISTGLNSVATSWTLDIQDVISKNMSDNRRTKIAQFVSLAVGLFSIG
                     VSIVMAHSDIKSAYEWFNSFMGLVLGLLGGVFILGFVSKKANKQGAYAALIVSTIVMV
                     FIKYFLPPTAVSYWAYSLISISVSVVSGYIVSVLTGNKVSAPKYTTIHDITEIKADSS
                     WEVRH"
     gene            4209..4667
                     /locus_tag="VW411_001593"
     CDS             4209..4667
                     /locus_tag="VW411_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU9794296.1"
                     /translation="MIISEQSDFKRYASVNKYFSKVCDFLENTNLTDLVDGKIDIDGE
                     NVFANCMTYLADGVPGDIFETHKKYLDIHIVVENTEKMAVTSPVRAQSRVPFSEEKDI
                     AFYDSKDYQIVELLPGNMLVTFEEDLHQPKIHCNDETVKKLVIKVLNEEK"
     gene            4680..6890
                     /locus_tag="VW411_001594"
     CDS             4680..6890
                     /locus_tag="VW411_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEU9794297.1"
                     /translation="MKQYVTLGTVVVLSAFVANSVAAQETETSEVSTPKLVQPVAPTT
                     PISEVQPTSDNSSEVTVQPRTVETTVKDPSSTAEETPVLEKNNVTLTGGGENVTKELK
                     DKFTSGDFTVVIKYNQSSEKGLQALFGISNSKPGQQNSYVDVFLRDNGELGMEARDTS
                     SNKNNLVSRPASVWGKYKQEAVTNTVAVVADSVKKTYSLYANGTKVVEKKVDNFLNIK
                     DIKGIDYYMLGGVKRAGKTAFGFNGTLENIKFFNSALDEETVKKMTTNAVTGHLIYTA
                     NDTTGSNYFRIPVLYTFSNGRVFSSIDARYGGTHDFLNKINIATSYSDDNGKTWTKPK
                     LTLAFDDFAPVPLEWPREVGGRDLQISGGATYIDSVIVEKKNKQVLMFADVMPAGVSF
                     REATRKDSGYKQIDGNYYLKLRKQGDTDYNYTIRENGTVYDDRTNRPTEFSVDKNFGI
                     KQNGNYLTVEQYSVSFENNKKTEYRNGTKVHMNIFYKDALFKVVPTNYIAYISSNDHG
                     ESWSAPTLLPPIMGLNRNAPYLGPGRGIIESSTGRILIPSYTGKESAFIYSDDNGASW
                     KVKVVPLPSSWSAEAQFVELSPGVIQAYMRTNNGKIAYLTSKDAGTTWSAPEYLKFVS
                     NPSYGTQLSIINYSQLIDGKKAVILSTPNSTNGRKHGQIWIGLINDDNTIDWRYHHDV
                     DYSNYGYSYSTLTELPNHEIGLMFEKFDSWSRNELHMKNVVPYITFKIEDLKKN"
     gene            6930..8039
                     /locus_tag="VW411_001595"
     CDS             6930..8039
                     /locus_tag="VW411_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU9794298.1"
                     /translation="MVNYGIVGAGYFGADLARSMNKIEDAKVVAVFDPNHGEEVAQEL
                     GSDVCASLDELVAREDIDCVIVASPSYLHREPVVKAAQHGKHVFCEKPIALSYEDCKA
                     MVDACKENNVIFMAGHIMNFFNGVHHAKELITQGKIGKVLYCHAARTGWEEQQPTVSW
                     KKLRSQSGGHLYHHIHELDCIQFIMGGLPEKATMVGGNVYHKGENFGDEDDMLIVNLE
                     YSDDRYAVLEYGNAFRWGEHYVLIQGTEGAIKLDLFNTGGTLRVKGEGESHFLVHETQ
                     EEDDDRTAIYTGRGMDGAIAYGKPGVRCPLWLQTCIDKEMEYLHDIIKGGEITEEFEK
                     LLNGVAALESIATADACTLSVKEDRKVSLSEITNA"
     gene            8196..9086
                     /locus_tag="VW411_001596"
     CDS             8196..9086
                     /locus_tag="VW411_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9794299.1"
                     /translation="MKIIGIDIGGTTIKADLYDEFGTSLNHFKEIETIIDYDLGTNQI
                     LNQVCDLIGEYTLNHSIDGVGISTAGVVNANTGEIIYAGYTIPGYIGVNFTAEIEKRF
                     GLYTFVENDVNCAALGELWKGQAKDKKNVVMVTIGTGIGGSIIVNGQIVNGFNYTAGE
                     VGYIPVGNSDWQSKASTTALIHLYQKKSLKTNQTGRTFFTDLRSGDKVAEETFEIFVE
                     NLTKGLLTISYLLNPEILILGGGILDSKDILLPEIQSSLAKNAMDNRFLPKNLVAATL
                     GNEAGRIGAVKNFLDRISNK"
     gene            9111..9434
                     /locus_tag="VW411_001597"
     CDS             9111..9434
                     /locus_tag="VW411_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794300.1"
                     /translation="MTNSVFSTMQDIENVATDIIKSYDNEIYTYKAVSQEELEKLEKS
                     YDEKSHEELVSIESNLEMKQQNLIDEVNKTIKENDANIQYISSSRRGEFVEKIIGRVV
                     EKYGH"
     gene            9424..11415
                     /locus_tag="VW411_001598"
     CDS             9424..11415
                     /locus_tag="VW411_001598"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEU9794301.1"
                     /translation="MAISQMKRISLLFSKSSLDDVLKTIQELESVQFRDLKVQDNWSE
                     ALEKDEVVFPTIQIFHTSNSNHGVIEGNDALTYLMNQQQHLEATVEKLQEYLPKENTF
                     KLLQQPPITTSYEELEKFGKANVAEGVLKKVNHQINRVHELERHIQSNNEEIERLIKW
                     EKLEIVPANLEQFSFCKGKVGTIPRTEDNRLYNSLLENNIEVQEIFSNDREYGVVVFY
                     QSSYSIDFDEYLFEPFDYSRKELPKQRVVDLDQENMQLITEKENIIASLQDSKKYLID
                     LQWQIDYILSIYARQISKNNFLCTPHLVALEGWIEETRILYFIKVMDEHFGHSIYIYE
                     SETLTDNQDEIPIKLTNHSLIEPFELLTEMYALPKYYEKDPTPVLAPFYFTFFGMMVA
                     DLGYGLLLFLGTMLALKIFHLPSATKRFLKFFNILGVAVAIWGGIYGSFFGYELPFHL
                     ISTTSDVMTILVVSVVFGFITVFAGLLASGLQKVRMNKYAEAYNSGFAWCVILLGLLF
                     IAVGMLMPDMRPLFVLGKWVSIFNAVGILIVSIIQTKSLSGIGAGLFNLYNISSYIGD
                     LVSFTRLMALGLSGASIASAFNLIVGLFPGILAKLTIGLVLFILLHAINIFLSLLSGY
                     VHGARLIFVEFFGKFYEGGGKPFQPLKASEKYIKVITKN"
     gene            11431..11907
                     /locus_tag="VW411_001599"
     CDS             11431..11907
                     /locus_tag="VW411_001599"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEU9794302.1"
                     /translation="MEHLATYFSTYGGAFFAALGIVLAVGLSGMGSAYGVGKAGQSAA
                     ALLKEQPEKFASALILQLLPGTQGLYGFVIGILIWLQLTPELPLEKGVAYFFVALPIA
                     IVGYFSAKHQGNVAVAGMQILAKRPKEFMKGAILAAMVETYAILAFVVSFILTLRV"
     gene            11941..>12408
                     /locus_tag="VW411_001600"
     CDS             11941..>12408
                     /locus_tag="VW411_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794303.1"
                     /translation="MSNLENLRESVIEQAHEKGRMKLLDSKKKIDDEFEMQKSLIIKK
                     KEAEHERKLKELQQKYQIIFQQLKNKERQSTLVSKQKILKELFQSALLEMESWSADKE
                     MEFIYRILERYSQQEVIVTFGERTLAKFNLEQLEKLKFSFPNYLFSEQPISNES"
BASE COUNT         4220 a         1775 c         2445 g         3968 t
ORIGIN      
        1 tataattacg ataaaaagaa taaaaactat tatattttta tgtttgttgt tctgttatga
       61 aacgaaagaa attggaaact agtttctaat ttatgaaaaa tattgaaaaa aaaaaagtgt
      121 taaaataaag aatgtaaacg tttacttcaa ctaaggagct catatgttac tgcaaaaaga
      181 actaattcca atgatagaag ctaacttacc aaatatggca tatgctgaaa aagacattgc
      241 taaattcttc ttaaaacagc aacctctgaa tgattattca tctaaagcat tgtgcgaata
      301 ccttaatgta tccaaagcaa cattgactcg atttgcgaaa aaatgtggtt ttaaaggttt
      361 tagacaattc attttcaaat accaagagat gattcatgag aaagaaaagt tggcattata
      421 tacagaggca acagaaaaag ttttatccga ctatgaggaa atgttgagaa aaacttacac
      481 ggttcttgat gaagttcaac ttgagcgtat tgctgagatg atagaaactg ctgagcgtgt
      541 atatctctac ggtaaaggaa gttctgttct tgctttacaa gaaatgaaga tgagatttat
      601 gcgtctcgga gtgattggtg aagtattatc agacgaggat atgattttgt ggagtagctt
      661 actacttaat gaaaattgcc ttgtcattgg agcatccatt tcaggtcaaa ctgatattgt
      721 actagaaggt ctacaaaaag ctgcagataa aggcgctaaa acagttttaa tgactacaag
      781 aaaatttgac gaagaagatt gtttctttga tgaactattg ttattagctt cgaccgatca
      841 tctctcgtat ggcaatcgca tatcacctca gtttccaata cttttaatta cagactgctt
      901 attctctaat tatctggaaa gtccagagag acaatattat tacaatcaaa ctattatcca
      961 taaggaggaa taataaaatg agccaaattt ggactaaaga aaaatttata agccaagttc
     1021 aaggtggagt cattgtttct tgtcaagctt tacctggtga agccctttat aatgaagaat
     1081 ttagcttgat gccttttatg gctaaagcag ctttagaggc aggagcagtg ggcattcgcg
     1141 caaattctgt gcgtgatatt aaagcaattc agaaagtagt agatttacca ataattggaa
     1201 ttatcaaaag ggattatcca cctcaagaac catatattac tgctacgatg aaagaagtag
     1261 atgaacttgt agaatgcgga acaacagtca ttgcatttga tgcaacttta agaccaagat
     1321 atgatggctt agttgtcagt gaatttatca aaaaaataaa agaaaaatat ccgaatcaat
     1381 tgctgatggc ggatgtaagt aatttagatg aaggtctcta tgcatttaaa tcaggcgttg
     1441 attttgttgg tacaacatta tcaggttaca caagtacaag tgtacaatca gatgagcctg
     1501 attttgaact aatgaaaaaa ttggctgatt ttaatattcc ggtaattgcc gaaggaaaaa
     1561 ttcattatcc agaacaatta aaaaaagctt atagtttagg tgttaccagt gtagtcattg
     1621 gtggagcgat tacacgtcca aaagaaattg ctcagcgatt tattaatgtc atcaaataaa
     1681 aaacaatata aaggagaaat ctgatgaaag atttaactaa atacaaaggc gttatccctg
     1741 cattttatgc ttgctatgat gaaaatggtg aaattagcca agatcgtgta aaatctctgg
     1801 tacaatattt cattgacaaa ggtgtaaaag gtatctatgt aaatggttct tcaggtgaat
     1861 gtatttacca aagtgtagaa gatcgtaaac aaattattga agctgttatg gaagttgcta
     1921 aaggtaaatt aacagttatc aaccatattg catgtaataa cacgaaagat agtatcgaat
     1981 tggcaaaaca ttcagaaagt gttggagtcg atgctattgc agctatccca cctatttatt
     2041 tcaaattgcc agagtattca atcgcagcat attggaatgc aatgagtgaa gctgcgtcaa
     2101 atacagattt tattatctat aatattccac aattggcagg ggttgcgttg actggtagtt
     2161 tgtatgcaac aatgcgtcaa aatcctcgtg tgattggagt taaaaattct tctatgcctg
     2221 tacaagatat tcaaatgttt gtagctgcag gtggagaaga ttacattgta ttcaatggtc
     2281 cagatgaaca atatcttggt ggtcgcttga tgggagcaga agctggtatt ggtggtactt
     2341 atggcgttat gccagatttg ttcttgaaat tggaaagttt gattcaagaa cgagatttag
     2401 atacagctaa aaaacttcaa tatgctatca atgaagttat ctataagatg atatcaggta
     2461 aggcaaatat gtatgctgta gcaaaagaag ttttgcgtct aaatgaaaaa cttgatttag
     2521 gttctgttcg tcaaccttta gaagcattgg cagaaggtga cttggaagtt gcaaaacaag
     2581 cagcagaact tattcaacaa gcacgaaaag aatttttata ataaataaaa tcaatatgga
     2641 gatatgttat gggcacaaca ggatttacaa taattgactt aattatcttg attgtttatt
     2701 tacttgcggt gttggttgca ggtatctatt tctctaaaaa agagatgaaa ggaaaagagt
     2761 tctttaaagg agatggttcg gttccttggt atgttacttc ggtatccatt tttgccacaa
     2821 tgctcagtcc gatttccttc ttgggactcg ctggtagctc ttatgcaggt agctggattt
     2881 tatggtttgc tcaattaggg atggtagtag ctattccact gacaattcgt tttatcttac
     2941 ctatctttgc acggatagac atcgatacgg catatgatta cttggataaa cgttttaatt
     3001 ctaaagcact tcgtattatt tcagcactct tgtttattat ttatcaattg ggacgtatgt
     3061 ctatcattat gtacctccca tcagctggtt tatcagtatt gacaggaatt gacatcaata
     3121 ttttgattat tttgatgggt gtagttgcaa ttgtttattc ttatactggt ggtctaaaat
     3181 ccgtattatg gacagacttt attcaaggtg tgattctgat tagtggtgtc gttttagctt
     3241 tatttgtact gattgctaat attaaaggtg gctttggtgc agtagcagaa acattagcaa
     3301 acgggaaatt ccttgctgca aatgaaaaac ttttcgatcc taacttgctt tcaaactcca
     3361 tctttttaat tgtgatgggt tcaggcttta caatcttgtc ttcctatgct tcatctcaag
     3421 atttggttca acgttttact acaacacaaa atattaagaa acttaataag atgttgttca
     3481 caaacggtgt tttgtcactt gcaactgcaa cagtctttta cttgattggt acaggcttgt
     3541 acgtattcta tcaagtacaa aatgcagata gtgcagctag caatatccct caagaccaaa
     3601 tctttatgta ctttattgca taccagttac cagtaggtat cacaggtttg atcttggcag
     3661 cgatttatgc agcatctcaa tcaactattt caacaggttt gaactctgtt gcaacttcat
     3721 ggacattgga tattcaagat gtcatttcta aaaatatgtc agacaatcgt cgtacgaaaa
     3781 ttgcacaatt cgtatctcta gcagtaggtt tattctcaat tggtgtttcc attgtcatgg
     3841 ctcactcaga tattaaatct gcatacgaat ggttcaatag tttcatggga cttgtacttg
     3901 gtctacttgg tggtgtattt attcttggat ttgtttctaa aaaagcaaat aaacaaggtg
     3961 cttatgcagc gctgattgta tcaaccatcg tcatggtatt tattaaatac ttccttcctc
     4021 caacagctgt tagctactgg gcatattcat tgatttcaat ctctgtatca gtagtttcag
     4081 gttatattgt atctgttctt actggaaata aagtatctgc acctaaatat acaacgattc
     4141 atgatattac agaaattaaa gcggattcaa gttgggaagt tcgtcactaa tagaaaagag
     4201 aaaataatat gattatttct gaacaatctg attttaaacg atatgcttct gtcaataaat
     4261 atttttcaaa agtctgtgat tttttagaaa atacaaattt gacagattta gttgatggaa
     4321 aaattgatat tgacggggaa aatgtttttg caaattgtat gacttatcta gctgatggag
     4381 ttccaggaga tatttttgaa actcataaga aatatttaga tattcatatt gttgttgaaa
     4441 atactgaaaa aatggcagta acttccccag ttcgtgccca gtcacgtgta ccttttagcg
     4501 aagagaagga tattgctttt tacgatagca aagactatca aattgttgaa ttgcttccag
     4561 ggaatatgtt ggtgactttt gaagaagatc ttcaccaacc aaagattcat tgcaatgatg
     4621 aaactgttaa aaaacttgtt atcaaagttt taaacgagga aaaataaaat gaaaaaaata
     4681 ttaaacaata tgtaacctta ggtactgtag tggtattatc agcatttgtt gctaactcag
     4741 ttgcagctca ggagactgaa acttctgaag tatcaacacc aaagttggtg caacctgttg
     4801 caccaacgac tccgatttcg gaagtacaac ctacatcgga taactcttcg gaagttactg
     4861 tacaacctcg aacagttgaa actactgtta aggatccatc ttctacagcg gaagaaactc
     4921 ctgtcttaga aaaaaataat gttactttaa cagggggcgg agaaaatgtt actaaagagt
     4981 taaaggataa atttactagc ggtgacttta ctgtagtgat taagtacaat cagtcaagtg
     5041 agaaaggctt acaagctctg tttggaatat ctaattccaa acccggtcaa caaaatagtt
     5101 atgtagatgt gttccttaga gacaatggtg agttggggat ggaagcgcgt gatacttctt
     5161 ccaataaaaa taacctagta tccagacctg cttcagtttg gggtaagtac aaacaagagg
     5221 ctgtgactaa cactgttgca gtagtagcag attcagtcaa aaaaacatat tctttatacg
     5281 caaatggtac aaaagtagta gaaaagaaag tggataattt cctaaacatc aaggatatta
     5341 aaggtattga ttactatatg cttgggggag tgaaacgtgc aggaaaaacg gcgtttggtt
     5401 ttaacggaac actagaaaat atcaaattct ttaatagtgc attggatgaa gaaactgtta
     5461 aaaagatgac aacaaacgct gttactggac atttaattta tacggctaat gatacaacag
     5521 gttctaacta tttccgtatt ccagttctgt atacttttag caatggtcgg gtattttcaa
     5581 gcattgacgc tcgttacggt ggaactcatg atttcttgaa taaaattaat attgctacaa
     5641 gttatagtga tgataatggt aagacatgga ctaaaccaaa attaacattg gcattcgatg
     5701 attttgcgcc agtaccatta gaatggcctc gtgaagttgg tggacgtgac ttacaaatca
     5761 gcggtggtgc aacctatatt gactctgtta ttgttgaaaa aaagaacaaa caagtactca
     5821 tgtttgctga tgtgatgcct gctggagtaa gttttagaga agcaactaga aaagattcag
     5881 gttataaaca aattgatggt aattattacc ttaaattaag gaaacaaggt gatactgatt
     5941 acaattatac tattcgtgag aatggtactg tatacgacga tcgtaccaac agaccaactg
     6001 aattttcagt agataaaaat ttcggtatta aacaaaatgg taattatttg acggtagagc
     6061 aatattcggt ttcatttgaa aataataaaa agactgaata tcgtaatggg actaaagtcc
     6121 atatgaatat cttctacaaa gatgcattgt ttaaagtagt gccaacgaac tatattgcat
     6181 atatttctag caatgatcat ggcgaatctt ggtctgcacc aactttatta cctcctataa
     6241 tgggacttaa tcggaatgcg ccatatttag gtcctggacg tggaatcatt gaaagctcaa
     6301 ctggacgtat tcttattccg tcttacactg gtaaagagtc tgcgttcatt tatagtgacg
     6361 ataatggagc atcttggaaa gttaaagtag tgccacttcc ttctagttgg tcagcagaag
     6421 cacaatttgt agaattgagt ccaggagtaa ttcaagcata tatgcgtaca aataatggta
     6481 aaattgcata tttaacaagt aaagacgcag gtactacttg gagtgcaccg gaatatttga
     6541 aatttgtttc aaatccaagt tatggaacac aattatcaat catcaattat agccaattga
     6601 ttgatggtaa aaaggctgtc attttaagta ctccaaactc cacaaatggt cgtaaacacg
     6661 gacaaatttg gattggtcta attaatgatg ataatacaat tgattggcgt tatcatcacg
     6721 acgttgatta tagtaactat ggatactcat attcaacatt gacagagtta ccaaatcatg
     6781 aaattggatt gatgtttgaa aaatttgatt catggtctcg taatgaactt catatgaaaa
     6841 atgttgtacc atatataaca tttaagattg aagatctgaa aaagaattaa agctgaaatt
     6901 tgaaaatata taaaaagagg ataaaaatta tggtaaatta cggtattgtt ggagctggat
     6961 attttggagc tgatttagct cgctcaatga acaaaattga agatgcaaaa gtggttgcgg
     7021 tatttgaccc aaatcatgga gaagaagttg ctcaagagtt gggatcagat gtttgtgcaa
     7081 gtttagatga acttgtagca cgtgaagata ttgattgtgt gatcgtagct tcacctagct
     7141 accttcaccg tgaaccagtt gtgaaagctg ctcaacatgg caaacacgta ttttgtgaaa
     7201 agccaattgc attgtcttat gaagattgta aagccatggt tgacgcatgt aaagaaaata
     7261 atgtcatctt tatggctggt cacatcatga acttctttaa cggtgtacac catgctaaag
     7321 aattgattac tcaaggtaaa atcggtaaag ttctttattg ccatgctgct cgtacaggtt
     7381 gggaagaaca acaaccaact gtatcatgga agaaacttcg ttctcaatct ggaggacatt
     7441 tgtaccacca tattcatgaa ttagattgca ttcagtttat catgggagga cttcctgaaa
     7501 aagcgacaat ggtaggaggc aatgtatatc ataaaggtga aaactttggt gatgaagatg
     7561 atatgctcat tgtaaactta gaatactctg atgatcgtta tgctgttttg gaatatggta
     7621 atgctttccg ttggggtgaa cactacgtct tgattcaagg aactgaagga gctatcaaac
     7681 ttgacttgtt caatactggc ggtactcttc gtgttaaagg tgaaggagaa tcacacttct
     7741 tagttcatga aactcaagag gaagatgatg atcgtacagc tatctatacc ggtcgtggta
     7801 tggatggagc aattgcgtac ggtaaaccag gagtacgttg cccattatgg ttgcaaacat
     7861 gtattgataa agaaatggaa tatctacatg acatcattaa aggtggagaa attacagaag
     7921 aatttgaaaa acttctcaat ggtgtagctg ctttagaatc aatcgctacc gctgatgcat
     7981 gtactttatc agttaaagaa gatcgaaaag taagtctttc agaaatcaca aatgcttaac
     8041 ttttgtaaaa cagaatagta aattcttgtc attatataat ttctaaagtt ctgtgataca
     8101 actcattgaa taaagaaata gagatgggac tgggataatg cccagtccca ttttttatca
     8161 aaaagtaatg agatcaaaaa tgtgggagtg ttgaaatgaa gattataggt atcgatattg
     8221 gcggaacaac aattaaggca gatttatacg atgagtttgg aacgagtttg aatcatttca
     8281 aagagataga aacaattatt gactatgatt tgggaacgaa tcagatatta aatcaggtct
     8341 gtgatttaat tggtgagtat actttaaatc attcaattga tggtgttggg atttccactg
     8401 ctggagttgt taatgctaat actggagaaa tcatctatgc aggctataca ataccagggt
     8461 atatcggagt aaactttact gccgaaatag aaaaacgttt tgggttgtat acttttgttg
     8521 aaaatgatgt taattgtgct gcattaggtg aattgtggaa gggacaagcc aaagataaga
     8581 aaaatgtagt aatggttact attggaacag gtataggagg cagtattatt gtcaacggac
     8641 aaattgttaa cggatttaac tatactgctg gtgaagtagg ttatattcct gtaggtaatt
     8701 cggattggca aagtaaagcc tcaacaaccg cattgattca tttatatcaa aaaaagagct
     8761 tgaaaactaa tcaaactgga cgtactttct tcactgattt aagatctgga gataaagttg
     8821 ctgaagaaac ttttgaaatt tttgtagaaa atctaacaaa aggtttatta acgatttctt
     8881 atctacttaa tccagaaatt ctcatattag gaggtgggat tctggatagt aaggatattt
     8941 tgttacctga aattcaaagt tctttagcta aaaatgcaat ggataatagg tttttaccta
     9001 aaaatcttgt ggcagctaca ttaggaaatg aagctggtcg tataggagct gtaaaaaatt
     9061 tcttagatag aatttctaat aaatagtatg taagataagg aggtgtcaca atgactaact
     9121 ctgtattttc gacaatgcaa gatattgaga atgttgcaac cgatattata aaatcatatg
     9181 ataatgagat ttatacttat aaagctgttt cccaagaaga attggaaaaa ctagaaaaaa
     9241 gttatgatga aaaaagtcac gaagaattag tttcaataga aagcaattta gaaatgaaac
     9301 aacagaacct tattgatgag gttaataaaa caatcaagga aaatgatgca aatattcagt
     9361 atatttcatc aagtaggaga ggagaatttg tagaaaaaat tattggtagg gtggtagaaa
     9421 aatatggcca ttagtcagat gaaaagaatc tctctactat tttctaaaag tagtcttgat
     9481 gatgttttaa aaactattca agaactagag tcagtgcagt tccgtgattt aaaggttcag
     9541 gataactggt cagaagctct agaaaaagat gaagttgtat ttccaactat tcaaattttt
     9601 catacttcta attccaatca tggggttatt gagggaaatg atgccttgac ttatttgatg
     9661 aatcaacaac aacatttaga agcaactgta gagaaattac aagaatacct accgaaagaa
     9721 aacacgttta aattattgca gcaacctccg ataactacct cttatgaaga attagagaaa
     9781 tttggtaaag ctaatgttgc tgagggtgtt cttaaaaaag tgaatcatca aattaacaga
     9841 gttcatgaat tagaaagaca cattcaaagt aataatgagg aaatagagcg attaataaag
     9901 tgggaaaaat tagaaattgt tcctgcgaat ttagaacaat tttctttctg taaaggaaaa
     9961 gtcggaacaa ttccaaggac tgaagataat cgcttataca atagtctttt agaaaacaat
    10021 attgaagttc aagaaatatt ttctaatgat agagagtacg gtgttgttgt tttctatcag
    10081 tctagttact ctatagattt tgatgaatac ttatttgaac catttgatta ttctagaaag
    10141 gaattaccga agcagcgagt agtagattta gatcaagaaa acatgcagtt aataactgaa
    10201 aaagagaata ttatcgcatc gttgcaagat tcaaagaaat atttgataga tttacaatgg
    10261 caaatagact atattttatc tatctatgct cgtcaaatct ctaagaataa ctttttgtgc
    10321 actccgcatc tagttgcatt agaaggatgg atagaagaaa ctcgtatttt atattttata
    10381 aaagttatgg atgagcattt tggacattct atttatattt atgaatcgga aacattgacg
    10441 gataatcaag atgaaatacc tatcaaatta acgaatcatt ctttaattga accatttgaa
    10501 ttattgacag aaatgtatgc tctgcccaaa tattatgaga aagatcctac acctgtatta
    10561 gcaccatttt actttacatt ttttggaatg atggttgctg atttaggcta tggtttacta
    10621 ttgtttttag gaacaatgtt agcattaaaa atttttcatc taccttcagc aactaagaga
    10681 tttttaaaat tctttaatat attaggggta gccgttgcaa tttggggtgg aatctatggc
    10741 tcattttttg gatatgagtt gccatttcat ctgatatcta caacctctga tgtcatgact
    10801 atattagtag tgtcagttgt gtttgggttt attacagtat ttgcaggttt gttagcttca
    10861 ggactacaaa aagtaagaat gaataaatat gcagaagcat ataattcagg atttgcgtgg
    10921 tgtgttattc tgcttggctt gttatttatt gctgttggaa tgttgatgcc tgatatgaga
    10981 ccgttatttg tattagggaa atgggtatct atttttaatg ctgtggggat tttgattgtt
    11041 tctattattc aaaccaaaag cttgtcaggt attggagcag gattgtttaa tctatataac
    11101 atttcatctt atataggtga tttagttagt ttcactcgat tgatggcatt aggattatct
    11161 ggagcaagta tagcatcagc tttcaattta attgttggtt tgtttccggg aatattggct
    11221 aaactgacaa ttggattagt attattcatt cttttacatg cgatcaatat ttttctatcg
    11281 ttactatcag gatatgttca tggagcacgt ctgatatttg ttgaattttt tggtaagttt
    11341 tatgagggtg gaggaaaacc atttcaacct ttgaaggctt ctgagaaata tattaaggtt
    11401 attacaaaga attaatggag gatatatata atggaacatt tagcaactta tttttcaacc
    11461 tatggaggag ctttcttcgc tgcattggga attgtattgg cggttggatt aagcggtatg
    11521 gggtctgctt atggagttgg taaggctggg caatctgccg cagctttact gaaagaacag
    11581 cctgaaaagt ttgcctcagc tttgatattg caattattgc ccggaacaca aggattatat
    11641 ggttttgtta ttggaatttt aatttggttg caattaactc cagaacttcc tttagaaaaa
    11701 ggcgttgctt atttctttgt agctcttcca attgctattg taggatactt ttcagctaag
    11761 catcaaggaa atgtagcagt agcgggaatg caaatcttgg ctaaaagacc aaaagaattc
    11821 atgaagggag caattttagc tgccatggta gaaacctatg caattcttgc ttttgtcgta
    11881 tcattcattt tgacccttcg tgtataagaa ataaatttgc aattcaaagg aggtgtctaa
    11941 atgagcaatt tagaaaactt acgagagtct gttattgaac aagctcatga aaaagggcgt
    12001 atgaaattat tggattccaa aaagaagatt gatgatgaat ttgaaatgca aaagtcgctc
    12061 attataaaga aaaaagaagc tgaacatgaa cgaaagttaa aagaattgca acagaaatat
    12121 caaataattt ttcaacaatt aaaaaataag gaacgccaat caacgttagt atcaaaacag
    12181 aaaatattaa aagaactttt tcaatctgct ttactagaaa tggaatcttg gagtgcagat
    12241 aaagaaatgg agttcatcta tcgaattctg gaacgatatt cacaacaaga ggtcatagta
    12301 acctttgggg aacggacttt agctaaattc aatttggaac aattagagaa attgaaattc
    12361 tcttttccaa attatttatt tagtgaacaa cctatctcaa atgaatca
//
LOCUS       DAVKNL010000052        12209 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVKNL010000052 DAVKNL010000000
VERSION     DAVKNL010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12209
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.052"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(1..184)
                     /locus_tag="VW411_001601"
                     /pseudo
     CDS             complement(1..184)
                     /locus_tag="VW411_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(287..424)
                     /locus_tag="VW411_001602"
                     /pseudo
     CDS             complement(287..424)
                     /locus_tag="VW411_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(808..2004)
                     /gene="tuf"
                     /locus_tag="VW411_001603"
     CDS             complement(808..2004)
                     /gene="tuf"
                     /locus_tag="VW411_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU9794304.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(2926..3795)
                     /locus_tag="VW411_001604"
     CDS             complement(2926..3795)
                     /locus_tag="VW411_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU9794305.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTENPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKATEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(4019..4627)
                     /locus_tag="VW411_001605"
     CDS             complement(4019..4627)
                     /locus_tag="VW411_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9794306.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPEKPIPQPNPEHPSVPTPNPELPNQETPTPDKPTPEPGTPKTETPVNPDPEVPTYE
                     TGKREELPNTGTEANATLASAGIMTLLAGLGLGFFKKKEDEK"
     gene            complement(4585..5691)
                     /locus_tag="VW411_001606"
     CDS             complement(4585..5691)
                     /locus_tag="VW411_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEU9794307.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGRQEKLVESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(5802..6056)
                     /locus_tag="VW411_001607"
     CDS             complement(5802..6056)
                     /locus_tag="VW411_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794308.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRITNRLANDLNNVLKQV
                     DKDTPNTPTWLNSAASKAKDDDRVCKLLKTLIPGENYLSC"
     gene            complement(6414..6860)
                     /locus_tag="VW411_001608"
     CDS             complement(6414..6860)
                     /locus_tag="VW411_001608"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9794309.1"
                     /translation="MDETGIDRYLYRPYAGAPRGEKVYEKISGRRFERTSIVAGQVDG
                     EFIAPMIYKKSMTSDFFVEWFKTQLLPALKTPHVIVMGNAGFHPKNILDELCIQDKHF
                     FLPLPPYSPDLNPIEQAWAILKKKVTDVLREVPTIFECLECFFKTR"
     gene            complement(6933..7258)
                     /locus_tag="VW411_001609"
                     /pseudo
     CDS             complement(6933..7258)
                     /locus_tag="VW411_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            7492..9210
                     /locus_tag="VW411_001610"
     CDS             7492..9210
                     /locus_tag="VW411_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEU9794310.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDIEYLKEVKDINI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            9321..9668
                     /locus_tag="VW411_001611"
     CDS             9321..9668
                     /locus_tag="VW411_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU9794311.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(9947..10783)
                     /locus_tag="VW411_001612"
     CDS             complement(9947..10783)
                     /locus_tag="VW411_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9794312.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTNLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(10796..11425)
                     /locus_tag="VW411_001613"
     CDS             complement(10796..11425)
                     /locus_tag="VW411_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9794313.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(11435..12076)
                     /locus_tag="VW411_001614"
     CDS             complement(11435..12076)
                     /locus_tag="VW411_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9794314.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
BASE COUNT         3574 a         2539 c         2219 g         3877 t
ORIGIN      
        1 gcatcaattg taacaaattc tcctgcctct gtagccttca aatggcgggc aagaaagact
       61 ttctcttgct caaccgtcat ataagcatgg ttacgaccac cacgtgtttc ttgaaggaga
      121 gagtcgagtc cgaactcctc atatttttta catttcgcca aatcgttgtt tgattacagt
      181 ctaaagtgta ttgaatctat aacagtacac cttggctgct aaaatatttc tataaattag
      241 tttgactttc ctgatagagt tgttcacatc ttatttcaaa ccactataaa agttctataa
      301 tctctttata agatttgccc atcagacgaa atagaacgat ttgaaggcgt ttatgatatt
      361 tagctgtacg agagtctttt aaaagtgttt tgatggtttg gatttcttct ttaattgatt
      421 tcatattact attgtataat gctttttgat tttagtctag tattatactc ttcgaaaatc
      481 tcttcaaacc gcgtcagctt cgccttgtcg tatagatgtt actgacttcg tcagttctat
      541 ctacaaccta aaaacaatgt tttgagcagt ctgtagctaa ctttctatag taaaatgaaa
      601 taagaacaag acaaatcgat caggacagtc aaatcgattt ctaacaatgt tttagaagta
      661 gatgtgtact attctagttt caatatatta tagtttgctc tttgattttc attgagtatt
      721 agttcaatat ctaaaaaaag aaccttgcca agcaagattc ttttagtgtc taacttaaat
      781 aattgttctt ctgggaacta aatcgaatta agcttcgatt tctgtaacca tacctgaacc
      841 aacagtacgt ccaccctcac ggatagagaa tgtagtacct tgttctacgg cgattgggtg
      901 aatcaactca acgtcgattg tcacgttatc accaggcatt accatttcag tacctgctgg
      961 aagttcgatt gaacctgtaa cgtcagtagt acggaagtag aattgtggac ggtagttgtt
     1021 gaagaatgga gtgtgacgtc caccttcttc tttagtaagg atgtagactt cacctttgaa
     1081 tttagtgtgt gggttgattg aacctggttt agcgataact tgtccacgtt cgatttcatc
     1141 acgttgaaca ccacgaagaa ggacacctac gttatctcca gcaagacctt cgtcaagttg
     1201 tttacggaac atttcaacac cagtaacaac tgctttttga gtttcttctt tgataccaac
     1261 gatttcgatt tcgtcgttga ctttaacgat accacggtcg atacgtcctg aagcaactgt
     1321 accacgtcca gtgattgaga atacgtcctc gactggaaga agcaatggtt tgtcagtgtc
     1381 acgttctggt tctgggatat actcatcaac tgtgttcatc aattccataa cgatgtcttc
     1441 gtatttagag tcaccttcaa gagctttaag tgctgaacct tggataactg gaagatcgtc
     1501 acctgggaag tcgtattctg acaataggtc acggatttcc atttcaacca attcaagcaa
     1561 ttcttcgtcg tcaaccaagt caactttgtt catgaagacg ataaggtgtt taacaccaac
     1621 ctgacgtgaa agaaggatgt gctcacgagt ttgtggcatt ggtccgtcag ttgaagctac
     1681 tacaaggata gctccgtcca tttgagcagc accagtgatc atgtttttaa cgtagtccgc
     1741 gtgtcctgga gcgtcgatgt gagcgtagtg acgtttttca gtttcgtact caacgtgcgc
     1801 agtgttgata gtgataccgc gttcgcgttc ttctggagca gcatcgatag acgcatagtc
     1861 tttaggttgg ttaactgatg aaggcaagcg acgtgccaaa acagttgtga tagctgcagt
     1921 tagggtagtt ttaccgtggt caacgtgtcc gatagtacca atgttaacgt gtggtttact
     1981 acgatcgtat ttttcttttg ccatttgagt aaaagcctcc aataaaatat attttataga
     2041 tagacagtag gcaatacagt ctaactttcc ttactatttt atcaaatttc agctaaattg
     2101 caagtgtttt acaacgtttt tgtgaattat tcttggtaaa aatttaacta attaatagaa
     2161 agtactaaat agacttatcc ttcaaggagt tttcagcatt atccatccac tcacttgctg
     2221 aaaatgtaaa cagtctctgg tttatatagt aaaatgaaac aagaataggg caaatcgttc
     2281 aggacagtca aatcgatttc taacaatgtt ttagaagtag atgtgtacta ttctagtttc
     2341 aatctactat acaacctcaa aactgtgttt tgaacaacct gcggctagct tcttagtttg
     2401 ccctttgatt ttcattgagt ataaaattgc agataaaacg ataacaatca taggactcag
     2461 cagagtcaat gtaggtcgaa gaatagcccc cctaatcgct tattttatag taaaatgaaa
     2521 tagaaactga acattttgtt gaggaaggta aatcaattcc tcacaatgtt tgagaaagaa
     2581 gggaggacta ttccaacttc aacccactat aaagcagact tacaagatag ggaacccatc
     2641 tccgataata ttccgataat atatagtaga atgaaaactg cacaatttga ttggagaatt
     2701 caaaccaatt tctcacaatg ttttagaaac aagggtctat tatttcaact gcaacctact
     2761 atagtccatt gaagctggaa tagcccactg agacttctaa accactgctg gagaggagtt
     2821 tgtttagagt ttctttcatt tccctaaaaa atcaaaaaga gagaaccagt ctgatcctct
     2881 cttacttata gatggtgctc actcaaatgt taaaggagct atttcttaga gataaaggaa
     2941 tttgaataca gctactgccg caattgctgc tgcgataggt gctactactg gtacccaaga
     3001 ataccaccat tttgaatcgc ctttatgctc accaagaact gatttgggaa ggaaagcatg
     3061 aaggagacgt ggtcccaagt cacgggctgg gttcaaggca ggtcctgtag gtcctccaag
     3121 tgatgttacc aaagccataa cgaggaatcc aagtgccaag tgagccacag aaagtcctga
     3181 agcagtgtgt ggagccacct gtgctttaat agccaagtca gaaaaatcaa ctgtttgtcc
     3241 tgcttctgtt gccttttgtt tcatgaattg aagcacttca gcaccaaaga agtttttagt
     3301 caaaccaaga gctgcaaaga acaaaacaaa tgaaccaaca aactcattaa tcaaaccatt
     3361 gacagttgct gcatagcgac tttcttttgt accatggtca atacttgaaa tagttgagaa
     3421 agttcccaag atgttatttg ggttttcagt tttcaagtag aatggacggt atgttgccac
     3481 aactaaggct tggccaaaga tagcccccaa gacttgcgcg ataatgtaag gtaccacttg
     3541 tgcccaaggg aaaagaccgc taactgcaag ccctagagtg aaagcagggt tgatgtgatt
     3601 cccagatacg ttaccaaaca tcaaggctgg gatcataacc cccataccat aaccaacagc
     3661 gatgacgatc cagccacttt ggtgaccttt cgtaccttta agttcaacgt tggcaactgc
     3721 accattccca agaatgatca aaatggcagt tcccaaaaat tcagtggcat acttcagtgc
     3781 ccatgtgaaa tccatttgct agattctcct taaaattttt tagatattcc ttattttatc
     3841 aatttttacc ctatttagca actgatttct ggaaatattt ttaaaaaaat aggattcata
     3901 gtttcccagt tcaacagact tgtaagcgtt tttattcttt acgaaagacc tcgttctcaa
     3961 aaccaggaaa aaagcccaca tctgtgagct tttcctggtt cctagattct aaaatctatt
     4021 atttttcatc ttcttttttc ttgaaaaatc ctaatcctag accagctaac aaggtcatga
     4081 taccagcact agccaaggta gcattagctt ctgtacctgt gtttggcaat tcctctctct
     4141 tacctgtctc ataagtcgga acttctgggt ctggattcac tggagtttca gtttttggag
     4201 tacctggttc tggagttggt ttatctggtg ttggagtctc ttgatttggt agttctgggt
     4261 ttggtgtcgg aacacttgga tgctctggat ttggttgtgg gattggtttt tctggtgttg
     4321 ataaacggtc ataccttacc gttatttctt tatcactaga gtctgacgta acttcttgtg
     4381 attcaactgt tggaatatct ggatctttgt acttgtcaat cttaccagat ataacctcgt
     4441 cccagtttcc tgttgtccat tcaccgtagg ttacaactcc cgtgaccttg ttctcagttt
     4501 ttgtacggct taaggttaca ggttgaacaa catcttcttt tacattttgg ttcgtaactt
     4561 tatcaacgta atgaatgata cgcgttatag tcttcgtctc agtagaggtt gctgttttgg
     4621 gaaccactgt ttcctcaaca ttctcacggt agtaatagtc aactgttgca ccgtcttctg
     4681 gtacgcattt gcaggagttg ctaccaaggt gtatgttgtt ttccttgtga taactcggtc
     4741 ttctttgtcc tcagttgttg ttttcccttc aatagttttt gattctgtgg tatactcaga
     4801 acctatcgct aaatcagctt ttataacaga ctctaccaac ttctcttgcc taccttcttt
     4861 atagtaattg gctgttactg tagcagtggt tggcgcttcg ctttctctat aaactaaggt
     4921 cactgttcta ccttcgctta caatattccc agttaaactt gcagaatttg tatctgcttc
     4981 tttaaaagta taatattttc cgtcagtagt agtcatgcta ctgagttttt tatctgtgac
     5041 attatagctg gtaccaatca gttgtttttt attggtaatg taggttccgt cactttcttt
     5101 ttctccaatt ccagtatcat ttttcaatga tagcaaacgc ccttgttcat caacatagcg
     5161 aactttcaca ttttctgaga ttagttctgc caattctgag gtttttttct ttttcttgat
     5221 ttcttcggtt attttccctt tctcttcttc gggaatattt agttttggaa tgattttttc
     5281 aacaacggtt cgtgatggtt ccacagtatc ttggatgact gaaaagtcag ctagaattgg
     5341 gagattatac tgaacacggt gactttgagt gtttactcct actctttcat tattctctga
     5401 aaatacttgt acggtataag aaacaacatc ttttcctaat agaacatccc cagtagagaa
     5461 atagccgcct tttcctagtt tgctatctcc agagcccact tctttcctaa tcttatcaga
     5521 tagtttttta ccagtcagta cattcgttcg cacaatccct ttgtctaccc ctacaaagtg
     5581 ggagaacttt ttgaactctt cagaaccaga tctagcccaa ccattattaa gggcatttgc
     5641 ttttgtattt gtattctctc tcaaaagttt ggcgattaga attatattca tcggcactta
     5701 gagttgctgc tatatctgac tcttgaatac caacttcctt actaccattt ctagcggcag
     5761 tatatgtgta tttatctgtt tctacttcta gctgattatc cttaacatga taggtaattt
     5821 tctcctggta taagagtctt cagtagttta catactctgt catcatcttt agctttagaa
     5881 gcagctgagt ttagccaagt tggggtatta ggagtatctt tatcaacctg tttcaagaca
     5941 ttattcaagt catttgctag cctattggtt atcctcactg tgaaagtttt cttccagtct
     6001 gaaccacttt tattagactt agatatgctc atgaaatcct ggtcaacatt caacacaata
     6061 tcgccttgtt ttttagcatc ttccgttgct tctgcttcaa attgatcatg gagtttttgt
     6121 tgctctggtg tatagttttt ttatttacac gatttaatgt actatttttt attttagtat
     6181 taagaactcc attaatacga tttttaaact ctgcttcaaa aaatccctta atttgttggt
     6241 gcgcaagttc aacattcaaa cctttcttat acaagtcttc attctgatac aagtcttcat
     6301 tctgaacgat ttcggctaca cggtgaatgg tttgattgta taccgtcttc tcttaaccag
     6361 cagtatcctt agttttaatg actactcgat aggttccttt agaaccgtta tagtcatcta
     6421 gttttaaaaa agcattccaa acattcaaaa atagttggaa cttcccttaa tacatccgtc
     6481 actttctttt tcaagatagc ccaagcttgc tcaataggat tcaaatccgg tgaataaggt
     6541 ggtagaggta agaaaaagtg tttatcttgg atgcagagtt catccaaaat gttcttggga
     6601 tgaaaaccag cattgcccat gacaataaca tgaggtgtct tcaaagcagg taggagttgc
     6661 gttttgaacc actccacaaa gaaatcgctt gtcatgcttt tcttgtaaat catgggagct
     6721 ataaactctc cgtctacttg tcctgcaaca attgaagttc gctcaaaacg acgtccgcta
     6781 atcttttcat agactttctc ccctctagga gcccctgcat aaggacgata gagatagcgg
     6841 tcgattcctg tttcatcaat ataaacaggt gctaggtgct ttaaactatt aaaattctta
     6901 agaaataagg ctacttttct gggtcttgtt ccttaaagct cgtcatcttt ttttaaagtg
     6961 accttaatct gctttaaagc tgcccatact gaaggaacag cacaatcaaa atgtgccgca
     7021 atttcccgta aaaaagcatc tggatgagcc tctacaaagg ctttcaattc ctctaaagga
     7081 atctttcggt ttttgacgac tcgctttttc ctctctatgt gttcttgttc acgtctttct
     7141 tttcccacgt gaagagagtt ctgacgccaa caccaaaaaa cttggctgcc tcgacatggc
     7201 tgtgcccctc tttgatgtaa tctaatgctc gctgtttaaa atcagtacta tatgccatag
     7261 ctttattata atatgcttgt ttaaaactaa gtaactatat caatgttttg ttatgagaag
     7321 acttactttt tttactgaaa ttgaattttt cctagcctca acatcatata accccaaaat
     7381 tcaatctgac ctggtttgaa gagatgttcg aagcagataa ggttttacta aaactaaaaa
     7441 aaatgatata attaaactga ttaaatgttt taaaaataaa ggagaatttg aatgtcttac
     7501 caagaaaatt accagaaatg ggttgatttt gtggagcttc ctgactacct tcgtcaagat
     7561 ttggaaaata tggacgaaaa aactaaggaa gatgccttct atacaaatct tgaatttggt
     7621 actgcaggta tgcgtggctt ggttggtgct ggtacaaacc gtatcaacat ctacgttgtt
     7681 cgccaagcta ctgaaggatt ggctcgtttg attgagtcaa aaggtggaaa cgagaaagaa
     7741 cgcggtgtag caattgccta cgatagccgt cacttctcac ctgagtttgc ctttgaatct
     7801 gcggcagttc ttgctaaaca cggcatcaaa tcttacgtat ttgaaagcct tcgtccgact
     7861 ccagaactat catttgcagt tcgtcatctc aactgtttcg caggtatcat ggtcacagcc
     7921 agccacaacc ctgctccatt taacggttac aaggtttacg gtgaagacgg tggacaaatg
     7981 cctccacacg atgcggacgc tttgactact tatatccgtg caatcgaaaa cccatttgca
     8041 gttgaagttg ctgatgtgga aactgaaaaa gcttctggct tgattgaagt tatcggcgaa
     8101 gctgttgaca tagaatacct taaagaggtt aaggacataa acatcaaccc agccttgatt
     8161 gaagaatttg gtaaagacat gaagattgtc tacacaccac ttcatggtac tggtgaaatg
     8221 ttggctcgtc gtgctcttgc ccaagcagga tttgactctg ttcaagttgt tgaagcgcaa
     8281 gcaactgctg acccagactt ttcaactgta acatctccaa acccagaaag ccaagcagcc
     8341 tttgcccttg ctgaagaact tggtcgtcaa gttggtgcag atgttcttgt cgcaactgac
     8401 ccagacgctg accgtgttgg tgttgaagtt cttcaaaaag atggtagcta cctcaacctt
     8461 tcaggtaacc aaatcggtgc tatcatggct aaatacatct tggaagctca caaaaacgct
     8521 ggaactcttc ctgaaaatgc cgctctctgc aaatctatcg tttcaactga cttggtaacg
     8581 aagattgctg aaagctacgg cgcaactatg ttcaacgtct tgacaggttt caaatttatc
     8641 gctgaaaaaa tccaagaatt cgaagaaaaa cacaaccaca cttacatgat gggatttgaa
     8701 gaaagcttcg gttacttgat taaaccattt gtacgtgata aagatgccat ccaagccgtt
     8761 cttgtagttg ctgaacttgc tgcctactac cgttctcgtg gtttgacact tgctgacggt
     8821 atcgaagaaa tctataaaga atatggctac tacgcagaaa aaacaatctc tgttactctt
     8881 tcaggtgtcg atggtgctga acaaatcaaa gcgattatgg ctaaattccg caacaatgct
     8941 ccaacagaat ggaacgcaac agctatcact gtcgtagaag acttcaaggc acaaactgct
     9001 actgttgctg acggtactgt tacaaacttg acaactcctc caagtgatgt gttgaaatac
     9061 acacttgctg acggttcatg gattgccgtt cgcccttcag gtacagaacc aaaaatcaag
     9121 ttctacattg cagttgtagg ggaaaccaac gaagaatcac aagctaagat tgctaacatc
     9181 gaagcagaaa tcaatgcatt tgtaaaataa agacctataa aagatgagac caaccaacct
     9241 catctttttt cgtatcaaat ctaagcaatt ttagaaactt tatggcatac tagacttatc
     9301 aatgaaagcg aggtaatctc atggaacaat ttttagataa catcaaagac cttgaagtca
     9361 ctacagttgt gcgtgcgcaa gaagctcttg ataaaaaaga aactgcaacc ttctttatcg
     9421 gtcgcaaaac ttgcccttac tgccgtaaat ttgcaggtac attgtcaggt gtcgtagctg
     9481 aaaccaaagc tcacatttac ttcatcaata gtgaagaacc aagccaactc aatgatttgc
     9541 aagcattccg ctcacgctat ggaatcccaa ctgtaccagg ttttgttcat attacagatg
     9601 gacaaatcaa cgtccgttgc gactcttcaa tgtcagcaca agaaatcaaa gatttcgcag
     9661 gattgtaaaa aatcagctaa caagtcttaa aatcacaaaa acgcataatg taaggtgcaa
     9721 aaaccttgat attatgcgtt ttattgttcc taaaacagtt acatcagtat ttcattacag
     9781 aacattttaa ttttggaagt caaatcaatt tttaacaatg ttttagaaat cacagtgggc
     9841 tattccatat tcaattatcc atattcaatc gctattatcg ttttcctaga ttacaaaaaa
     9901 aactacgttt tgctaatcaa aacgcagttt cagccactat tttatcttac tgtccttctt
     9961 ttacttcttt ggttgctaca tcttctccaa accatttttg gctgatttct tggaacttgc
    10021 cgtccttgta aagactagaa aaagcttcat ttatcttctt aaccaagttt gtatcttcct
    10081 tacgggctcc aaccgcaaaa gcttctgttt ctagtccaac tgtaaagaca ttataatcgt
    10141 ttaaaacacc ttctgcttct aaataatagt ttgcatagac acggtcaatc aatagaccat
    10201 caattcgatc gtttttcaaa tcaatcaagg cttcattaaa ggtttggtat tgattcgctt
    10261 ccttattagc gacaatattc ttcaaaattt ctggatttgc ttcaaagtcc gcataaccag
    10321 atgaaccagc ttgagctcct aatgtctttc cagtcatatc ctttgcagtc gtgataccag
    10381 atgatttctt cgtaaccaat acctgctcat tcttcatata tgagttactg aaagccacct
    10441 tttcacggcg ttcgtctgta gcggaatagc cattccaaat cagatcaatc gttccttttg
    10501 tcaattcagc ttctttcaaa tcccaatcaa tcggttgcca atttaccgtg attccgtatt
    10561 tttcaaaaac agctgtagct aaatcaatat caaatcctgc ataagaacca tctttctgag
    10621 caaatcccat tggaacaaaa gtactatcaa atccaatagt aatagacttg ttagactggt
    10681 actttgacca attatctcca ctagtttcgc tagaattttt cccacaagct actaagaaca
    10741 aagcagtcat cagactgact aatacaagca tccatttttt catccatttt tcctcctatt
    10801 taggttctac tttcaataat acatctgcga tattttcagc aaactgcaaa tcatgggtaa
    10861 ccacaatctg ggtcatccca agttccctat tttgcaagat tagcttctcc acttccaaac
    10921 gtaattctgg atccagggca gaagttggtt catcgtagcc aatgatttct gggtcaatca
    10981 tcatagcacg cgccaaagcc acccgctgct tttgcccacc agatagtgag aaaggatagg
    11041 cctctgcgtg tcctcctagt cctaactgtt ccaagagtcc actcgccttc ttctcagcct
    11101 cttcctgctt cattcccatg gtcttcacag gcgataaagt caaattttcc agaactgata
    11161 gatgaggaaa tagttgaaaa tcttggaaga caaatcccag tagattgcgc ttctgcaatt
    11221 catccagctc taaaggttgt ccattataaa agatttgccc tgaatcaatg gtttcaagac
    11281 ctgcaagcat acgtaaaaga gttgtcttac ctccaccaga aggtccaacg atagccagga
    11341 tttgcttttc aggaatactt agactgaaat tagacaggat ttgtttgtct ccaaagactt
    11401 tattgatatt tcgtaattct aacatggcag cctcctatct ataataacta tacttcttct
    11461 caactttttt ggaaataatt gtcacaatcc caatcaaaat caaataaatg gctcctgcca
    11521 agaacatagg aactagacta gcatcgcggt tagcagctgt tcgactagcc aagataaggt
    11581 ctgaaattcc gagagcatag accaaagaag tatccttgac caaactcata acttcattaa
    11641 agacactagg aagaacgatc ttggtcactt ggggcaagat aatatagcgc actctgtcaa
    11701 aagggctaaa cttcaagacc ttggcggcct catactgtcc tcttggaata gtgtcaattc
    11761 ccccacggaa aatttctgca aagtaagctg catagttgag aacaaaggca ataatagctg
    11821 caggaaggcg gtctaaacga atcccaatac ttgggagcac ataatagata aaaatcagtt
    11881 gcaagagtaa gggggttcct cgcataaccc agatgtaaat gttaatcaga taatggaggg
    11941 gcttccaatg gacttgcaag gcaaaggcaa tcaaaacgcc caagggaatc gaaaatagca
    12001 agaccagtgc aaagacctgt acagtcgtgc tcgcaccatt cagtaaactc ggtaatatct
    12061 caaataaata agacatactg cacctcctaa aagtaattgt tcctattata gcatgaataa
    12121 acaagattac caagtatttt tgtaaaaaga ttctaccata tacaaataaa aaccgtagct
    12181 atcaaaagag ataaactaca gttctatat
//
LOCUS       DAVKNL010000053        11930 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVKNL010000053 DAVKNL010000000
VERSION     DAVKNL010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11930
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.053"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(37..1410)
                     /gene="mnmE"
                     /locus_tag="VW411_001615"
     CDS             complement(37..1410)
                     /gene="mnmE"
                     /locus_tag="VW411_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEU9794315.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEPLTAQDRQLLEISQETNRII
                     LLNKTDLPETIETSELPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(1547..1729)
                     /locus_tag="VW411_001616"
     CDS             complement(1547..1729)
                     /locus_tag="VW411_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEU9794316.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            1853..2452
                     /locus_tag="VW411_001617"
     CDS             1853..2452
                     /locus_tag="VW411_001617"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEU9794317.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPMITSNKEQNYDN"
     gene            2442..2921
                     /locus_tag="VW411_001618"
     CDS             2442..2921
                     /locus_tag="VW411_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9794318.1"
                     /translation="MTIELRDVTMENYFDVLNLDVKEYQKQFIATNAISLAEAYVYTK
                     NGDFVAPLAVYDNDAIIGFVMIAYDKKIGISSGNYLLFRFMIDKNFQNQGYFKPIMDK
                     VLDYVRTAPAGLSNKLWLSYEPENEQARFCYLSYGFKETGEISENEVVAIYDLTIEK"
     gene            2931..4010
                     /gene="prfA"
                     /locus_tag="VW411_001619"
     CDS             2931..4010
                     /gene="prfA"
                     /locus_tag="VW411_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEU9794319.1"
                     /translation="MNIYDQLQVVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEELAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            4010..4849
                     /gene="prmC"
                     /locus_tag="VW411_001620"
     CDS             4010..4849
                     /gene="prmC"
                     /locus_tag="VW411_001620"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEU9794320.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILAENPVKNLMVLDIGTGSGAIALALAKNRPDWSVTAADISQDALDVASENAKNQNLQ
                     IFLKKSDCFTEISEKYDIIVSNPPYISREDESEVGLNVLYSEPHLALFADEDGLAIYR
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKRVRTLKDQFGQDRMVVVDD
                     GQD"
     gene            4833..5435
                     /locus_tag="VW411_001621"
     CDS             4833..5435
                     /locus_tag="VW411_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEU9794321.1"
                     /translation="MMDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     RRDKALNLNVASLEDILYFSKNQPTYLQKLVEIFLPGPLTIILEANDRVPYWVNSGLA
                     TVGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFNQILEDFDQEVLGLEDDAFL
                     TGQDSTILNLSGDKVKILRQGAIKREDILARLPKISFEEE"
     gene            5437..5868
                     /locus_tag="VW411_001622"
     CDS             5437..5868
                     /locus_tag="VW411_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014947335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9794322.1"
                     /translation="MLRDLRETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEEEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIC"
     gene            5930..7186
                     /locus_tag="VW411_001623"
     CDS             5930..7186
                     /locus_tag="VW411_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU9794323.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPKTELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYESLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYED"
     gene            7194..8168
                     /locus_tag="VW411_001624"
     CDS             7194..8168
                     /locus_tag="VW411_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEU9794324.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSSKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAKSFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            8168..8782
                     /gene="pmp23"
                     /locus_tag="VW411_001625"
     CDS             8168..8782
                     /gene="pmp23"
                     /locus_tag="VW411_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEU9794325.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     QDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNRTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            8856..9830
                     /locus_tag="VW411_001626"
     CDS             8856..9830
                     /locus_tag="VW411_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEU9794326.1"
                     /translation="MRKKLFLTSAAILWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDIRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            9904..11535
                     /gene="rlmD"
                     /locus_tag="VW411_001627"
     CDS             9904..11535
                     /gene="rlmD"
                     /locus_tag="VW411_001627"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU9794327.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVIIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSQKNASLNKITNAHYVCDTAENAMKKWLKEGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            11689..11838
                     /locus_tag="VW411_001628"
                     /pseudo
     CDS             11689..11838
                     /locus_tag="VW411_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895229.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
BASE COUNT         3723 a         2284 c         2553 g         3370 t
ORIGIN      
        1 aaaatccatg accgaatgaa ggatcatgga tttttcttat tttcctaaac agaattggct
       61 aaagagttgg gtgatgagtt catctggagc agcatctcca gtgatttctc ctagaatttc
      121 ccaagtacgg gtcaagtcaa cttgaagcaa gtcaactggc atccctagtt caagaccttg
      181 gttaacagct tgtaggcttt caacggcctt ctcaatcaag gaaatgtgac gggcgtttga
      241 caagtaggta gcatcttgct caaccaaacc agcattttca aagaagaggt tgttgattct
      301 ctcttcgatt ttatcgatgt tttgattttt aagaactgaa atgcggatga catcttcagg
      361 tagttccgaa gtttcaatcg tttcaggcag gtcagttttg ttaagaagaa taatgcgatt
      421 agtctcctga ctgatttcta ggagttggcg atcttgggcg gttagtggtt cactagcgtt
      481 tagtactagc agaactaggt cagcttcctt aagagctttt ttcgaacgct caactccaat
      541 ttgttcaacg atatcatccg tttcacgaat accggctgta tcaatcaatt tgagaggtac
      601 accattgatg ttgacgtact cttcgatgac atctcgtgtt gtcccagcga tatctgttac
      661 gatagccttg tcctcacgca agaggttgtt gagaaggctt gatttcccaa cgttgggacg
      721 tccaatgata gccgttgaaa ttccttcacg aaggatttta ccacgacgtg ctgtcctaag
      781 gagtttggtt agtaattgct caaactccat tgtcttctct cggacaacag cagtagtggc
      841 ttcctcaaca tcgtcatact caggatagtc gatattgacc tcaacttggg caagtgtatt
      901 gaggatttct tgacgggtat tgttaatgag gtcagaaagg gagccgtcta attgtttgac
      961 cgcaatgttc atggccttgt cagtcttggc acggatgata tccatcacag cctctgcctg
     1021 tgtcaagtct acgcgaccgt ttaaaaaagc acgtttggta aattcaccag gttctgccaa
     1081 ccgagcccct tcacgaatag ctagctggag aatttcattg gtcaccgcaa tcccaccgtg
     1141 ggtgttaatc tcgataatat cctcacgagt gaaggtcttt ggagacttca tagccccaac
     1201 cataacctcg tccatgactt taccagtcag aggatcaata atgtgaccgt agttgagagt
     1261 gtggctggca accttgttca agtcttttcc tttaaaaatc ttttgcgcaa tagcaaaact
     1321 gtctgttccg ctcaggcgga caataccaat agccccttca cctagtggag tagagatagc
     1381 agcgatggta tcaaattcac gtgtaatcat aatgtttcct ttaattaata gctcttttct
     1441 tggaaattct ccaagtttct tttcaaattg taacaaattt aggatatttc ttcaatgaat
     1501 gctacttgga gattgaaaaa gcctaagcaa ttctgcttaa gctagcttat ttagtacgca
     1561 tttccccttg tgggaagtaa gttccttctg gcatgtcgtt gatgatgaca tggacagcag
     1621 attgaggggc tccagtgttg cggacaactg cttccgttac ttccttagca agagctttct
     1681 tttgctcgag cgtgcgtcct tcaaataaat cgatgcgtac aaatggcata atagcttcct
     1741 ccactagttt ttgatttctt ctattttacc acattttgcc gtttaaagct taagaaaatt
     1801 atgatatact agaatgtagc aaaaatttag aaatggacgt gaagcaagaa acatggcaca
     1861 gttgtactat cgttatggga ccatgaactc tggtaaaacg attgagattc tcaaagtggc
     1921 ctataactac gaggagcaag gaaaaggtgt tgtgattatg acctcggctc tggatacgcg
     1981 tgacggcgtt ggctatgtgt cgagtcgaat tggcatgaaa cgccctgccc ttgcgattga
     2041 ggaaacaacg gatatctttg gctatatccg agacctacca gaaaaacctt actgtgtttt
     2101 ggtcgatgaa gcccagtttc tcaaacgcca ccatgtttac gacctagctc gtgttgtgga
     2161 cgagttagac atacccgtca tggcttttgg cttgaaaaat gactttcgca atgaattgtt
     2221 cgaaggttcc aaatatctct tgcttttagc agacaagatt gacgaaatca agaccatctg
     2281 tcagtattgc aagaaaaagg cgaccatggt gttgcgaaca caggatggac tgcccgttta
     2341 cgatggagaa cagatccaga ttggtggtaa cgaaacctat atctcagttt gccgtaaaca
     2401 ttattttgcg ccaatgataa cctctaacaa ggagcaaaac tatgacaatt gaactaagag
     2461 atgttacaat ggaaaattat tttgatgttt taaatttgga tgtcaaggaa tatcaaaaac
     2521 aattcattgc aaccaacgca attagtttag ctgaagcata cgtctacact aaaaatggag
     2581 attttgtagc tccattggca gtttatgata atgatgcaat tataggtttt gtgatgatag
     2641 cttatgataa aaagatcgga attagtagtg gaaattattt actatttcgt tttatgattg
     2701 ataagaattt tcaaaatcaa ggatatttta aaccaattat ggataaagta ctagactatg
     2761 ttcgaacagc gccagcaggt ttaagcaata aactttggtt gtcttatgaa ccagaaaatg
     2821 aacaggcaag attttgttac ctcagttatg gatttaaaga aactggggaa atatccgaga
     2881 atgaagtagt agcaatctat gatttaacaa ttgagaaata aggagaaaaa atgaacatct
     2941 atgatcaact acaagttgta gaagaccgtt atgaagaatt aggagaattg ctgagtgacc
     3001 ctgatgtcgt ttcagacacc aagcgtttta tggagctttc aaaagaagaa gcttccaatc
     3061 gtgacaccgt aatagcctac cgtgagtata aacaagtcct tcaaaatatc gtcgatgccg
     3121 aagagatgat taaggaatca ggcggagatg cggacttgga agaattggcc aagcaagaac
     3181 tcaaagatgc caaggctgaa aaagaagaat atgaagaaaa actgaaaatt ttgctccttc
     3241 caaaggatcc aaacgatgac aagaatatca tccttgaaat ccgtggagca gctggtggag
     3301 acgaagcggc acttttcgct ggagatttgc taactatgta ccaaaagtat gcggaagccc
     3361 aaggttggcg ctttgaagtc atggaagcct ctatgaatgg tgtcggtggt tttaaagaag
     3421 tggttgctat ggtttcaggt cagtctgtat actctaagct taagtatgaa tcaggtgccc
     3481 accgtgtgca acgtgttcct gtgacagaaa gccaaggccg tgttcatact tcgacagcga
     3541 cagttcttgt tatgccagaa gttgaagagg ttgaatacga cattgatcca aaagaccttc
     3601 gtgtcgacat ctatcacgcc tctggtgctg gtggacagaa cgtcaataag gttgcgactg
     3661 ccgttcgtat cgttcacttg ccaaccaata tcaaggttga gatgcaggaa gaacgtaccc
     3721 agcagaagaa ccgtgagaag gccatgaaaa tcatccgtgc ccgtgttgct gaccactttg
     3781 ctcagattgc tcaagatgag caggacgctg agcgtaagtc gacaatcggt actggtgacc
     3841 gttcagaacg gatccgaact tataacttcc cacaaaaccg tgtcacagac caccgtatcg
     3901 gcttgaccct ccaaaaacta gatacgattt tgtctggtaa attggacgaa gttgtggatg
     3961 ccttggtgct ttatgaccaa acacaaaaac tagaagaatt aaacaaataa tgaaattagc
     4021 tcaattattt tcaaattttg aagaagagtt gataagacaa ggagaggaag cggaaagcct
     4081 ctcttttgtc tatcgtagtc tgaaaaatct atcttttaca gactttattt ttgccctcca
     4141 gcaggaggtg acaacagagg aagaaaaaca atttgtagaa gatatttacc agcaactagc
     4201 agctcacaag ccagctcagt acatcattgg acaggcagat ttttatggaa tgcatttaaa
     4261 ggtggatgaa cgggttttga ttcctcgtcc agaaacagag gagttggtgg agcttatcct
     4321 ggctgaaaat cctgtgaaaa atcttatggt cctagatatc ggaacaggta gtggagctat
     4381 tgctctcgct ctagcaaaaa acagaccaga ttggtcagtg acggcagcag atatttccca
     4441 agatgcttta gatgtagcta gcgaaaatgc taaaaatcaa aatcttcaaa tatttttaaa
     4501 aaaatctgac tgttttacag aaatttctga aaaatatgat ataattgttt ccaatccacc
     4561 ctatatctct cgtgaagatg agtcagaggt aggcttgaat gtcttgtatt cggagcctca
     4621 tctagctctc tttgcagatg aggatggcct agctatttac cgtagaattg cggaagatgc
     4681 aaaagactat ctcaaagatg gtggtaagat ttaccttgaa attggataca agcaaggtca
     4741 aagtgttcct gaacttttta ggaaacatct tcctgaaaag cgagtacgaa cactcaagga
     4801 ccaatttggt caagatagga tggttgtcgt tgatgatgga caggattaga caagagttgg
     4861 aaaatggtgg agctgtcgtt ttgcctacag agacagttta cggtctcttt gctaaggcgc
     4921 tagacgaaaa agcagttgac catgtttacc agcttaaacg tcgacgtaga gacaaggcgc
     4981 tcaatctcaa tgttgcctct ttagaggaca tcttgtactt ttcaaagaat cagccaactt
     5041 atcttcaaaa acttgtagag atctttttac caggtccctt gaccattatt ctcgaagcta
     5101 atgaccgagt tccctattgg gtcaattctg gccttgcaac tgttggattt cggatgccta
     5161 gtcatcccat tacccttgat ttgattcgag agacaggtcc cttgattggg ccgtctgcca
     5221 atatctcagg tcaggcgagt ggagtgacct ttaatcaaat tctagaggat tttgaccaag
     5281 aggttctggg tctggaagac gatgcttttc taactggaca ggattcaact attttgaatt
     5341 tgtcgggaga caaggtgaaa atcttacgtc aaggggccat taagcgagaa gatattcttg
     5401 ctcggttgcc aaagatttct tttgaggagg aatgagatgc taagagattt aagagaaact
     5461 gatgtgaaag ctatatgtga catcaaccaa gaggctttgg gttatacttt tagtccagag
     5521 gaaacggcta gccaactagc tagactgtct caggattccc atcatttcct acttggctat
     5581 gaggatgcag ctaatcatgt cttacttgga tatgtccacg ctgaagttta cgaatcactc
     5641 tattccaaag caggatttaa tatcttagct ttagcagttt cacctcaagc gcaaggtcaa
     5701 ggtattggta aaagtttact acaagggttg gaagaagaag caaaaagatg tggttatggg
     5761 tttatccgct taaattctgc caatcatcgt ctgggtgctc atgcatttta tgaaaaagtt
     5821 ggctatactt gtgataaaat gcagaaacgg tttattcgca tctgttagtt tgattttctt
     5881 attgtaaaat caaactaatg gactagtcac acaataaagg agaagaccta tgatttttga
     5941 caaagatgat tttaaagcat atgatgctga tctctggaat gctattgcca aagaagaaga
     6001 acgccaacaa aataatatcg agttaattgc ttcggaaaac gtagtttcca aggctgttat
     6061 ggcagctcaa gggtctatct tgacaaataa atatgccgag ggttacccag gacgccgtta
     6121 ttatggtgga actgatgtag tagacgttgt agagactctt gctattgaac gcgcaaaaga
     6181 aattttcggt gctaaatttg ccaatgttca accacattca ggaagccaag ctaactgtgc
     6241 ggcttacatg tccttgattg agccaggtga tacggttatg ggaatggatt tggcatcagg
     6301 tggtcatttg actcatgggg ctcctgttag cttctctggt caaacctaca actttgtttc
     6361 ttatagtgtt gatcctaaaa cggaactctt agactttgat gctatcttga aacaagccca
     6421 agaagtaaaa ccaaaactga ttgtagctgg tgcttcagcc tattctcaaa ttatcgattt
     6481 ttcaaaattc cgtgaaatcg cagatgctgt cggtgcgaag ctcatggtgg acatggccca
     6541 tatcgctggc ttggttgcgg ctggccttca tccaagccca gttccatacg ctcatatcac
     6601 aacaacaacg acccacaaaa cccttcgtgg acctcgtggt ggtttgattt tgaccaatga
     6661 cgaagaactt gctaaaaaaa tcaattcagc tattttccca ggtattcagg gcggtccttt
     6721 agagcatgtt gtggcggcta aggcagtttc cttcaaagaa gttttggatc cagccttcaa
     6781 ggaatatgct gccaatgtaa ttaagaacag caaggctatg gcagatgtct tcttgcaaga
     6841 ccctgatttc cgtattattt caggtggaac tgaaaaccat ctcttccttg ttgatgtgac
     6901 taaagttgta gaaaacggca aagttgctca aaacttgttg gatgaagtca atattacctt
     6961 aaataaaaac tcaatccctt acgaaagctt gtcaccattc aagacaagtg ggattcgtat
     7021 cggagcagca gccattactg cacgtggatt tggtgaagaa gaaagtcgca aagtggctga
     7081 actcatcatt aaaaccctta agaattcaga aaatgaggct gtattagaag aagtgagaag
     7141 tgcagtcaaa gaattgacag atgccttccc attatacgag gactaaaact tttatggaca
     7201 tttatattaa gaaagccatt attcaccagt tcagtccgga tgataccgaa ctgttcttag
     7261 cagataagtt tctcaatatt actccaaaaa tcgaagaata cctgcgtaaa aaaattgaac
     7321 atgtgtattc agatgaagcc aagactggga ttttcgaaga agaaaatccc ttcttcaatc
     7381 atattacaga cgatttgttg gagacatcag taacgctggc taatctctgg aaagaggagt
     7441 ttagcatttc tgaaaatctc aagaccaatg acttgatttt tgtgcaattt tctaaagaag
     7501 gtgtagaaca tttcgctttc ttgcgaattg ccctgcggga gaccttgacc cacctcggag
     7561 gagaagttga taatccaatc aagctaactc agaataacct gcctggattt ggaacaggag
     7621 ctgatgaggc cttggtggtc aatcttcaga gtagcaagta tcacctgatt gaaaaacgaa
     7681 tcaagtacaa cgggactttt ttgaactatt tttcagataa tcttcttgct gtcgctccta
     7741 agatttctcc taaaaaatct atcaaggaac tggaaaaaac agcccagaga attgctaaat
     7801 cttttaacac agatgatttt caatttcaat ccaaggtcaa atcagctatt ttcaacaacc
     7861 tagaagaaag caatgaattg tcacctgaga aattggctaa tgaccttttt gacaacaatc
     7921 tgacggctcg tttgagcttt attgaccaag tcaaagaagc cgtaccagaa cctgttcaat
     7981 ttgatgaaat tgatgccagt cgccaattaa agaaatttga aaaccaaaaa ctctccttat
     8041 caaatggaat tgagctcatc gttcccaata acgtctatca agacgccgag tctgttgagt
     8101 ttatccaaaa cgaaaatgga acctactcta tcttaatcaa aaatatcgag gatatccaaa
     8161 gtaaataatg tttaaacgaa ttcgaagagt gcttgtacta gcagtcttcc tttttgctgg
     8221 ctataaagct taccgcgttc atcaagatgt caaacaagtc atgacctatc aacccatggt
     8281 gcgagaaata ttgagtgaac aagacacccc agcaaacgaa gagcttgtgc ttgctatgat
     8341 ttatactgaa acaaaaggaa aagaaggcga tgttatgcag tctagtgagt ctgcaagtgg
     8401 ttccaccaac accatcaatg ataatgcctc tagcattcgg caaggcattc aaactctgac
     8461 aggcaatctc tatctggcgc agaagaaggg ggtagatatc tggacagctg ttcaagccta
     8521 taattttgga cctgcctata tcgattttat cgcccaaaat ggcaaggaaa ataccctggc
     8581 tctagccaaa cagtactctc gtgagactgt tgcccccttg cttggtaata ggactggaaa
     8641 gacttatagt tatattcacc ccatttccat ttttcacggt gctgaactct atgtaaatgg
     8701 aggaaactat tattattcta gacaggtacg acttaacctt tacatcatca aatgtttcac
     8761 tctcttttca acatctggct agaccaggtg tttttgttat aagttttctt taagatagat
     8821 atattactct aaagaagtaa aggaggaaat tccctatgag aaagaaactc tttctgacta
     8881 gcgctgcgat cttgtgggca gtaacagcta tgaatagcgt ccatgcagca acagatgttc
     8941 aaaaagttat cgatgaaacc tatgtccaac ctgaatatgt cctaggttcc tccctatctg
     9001 aagaccaaaa aaatcaaact cttaaaaaac tgggctacaa tgcctcaaca gataccaaag
     9061 aactcaagac catgacacct gatgtttatt ctaaaatcat gaatgtggcc aatgactcta
     9121 gcttacagtt gtattcatca gccaagattc aaaagctagg tgacaaatcg ccacttgagg
     9181 tcaagattga aacaccagaa aatatcacta aggtgactca ggatatgtac cgaaacgcag
     9241 cagtaacgct gggtatggaa catgccaaaa tcactgtagc agcccctatt ccagttacag
     9301 gagaaagtgc tttagcaggg atttactatt cgctagaggc taatggagcc aaggtgccac
     9361 aagctaataa agatttggct caagaagagc taaaagcttt gtcagatatc aatgctgaaa
     9421 acaaggacaa atcaggctat gatgctaata aattaaacgt tgccctagct gatatcaagt
     9481 caggactcgc caaagctaaa gaaagtaagg gaaatctgac agaagaagat atccgcaaga
     9541 ttgttgaaga taccttaaaa aattacaaac ttgatcaggt cataacagga aaccagatca
     9601 atatcatcat caattttgcc ttgaatctct caaagagtga tatcctcagc aatgcagatt
     9661 tcactaaaac cctaaatgac cttaaacaaa gcatcgtatc acaagctggc gacagtttta
     9721 aaaatatcaa ccttaacttt gatgcggata aggcgctaga ggacggtggt aacttcttaa
     9781 gctccctctg gcaagccctt gtcaacttct tcaagagttt tggttcttaa gaaatttcat
     9841 ggtataatgg atggtaaccg taacgttgcc gtcttttttg tcggccaata gaaagagaaa
     9901 agaatgttaa agaaaaatga tattgtagaa gttgaaattg ttgatttgac ccatgaaggg
     9961 gcaggagttg ccaaggtaga tggtttggtc ttttttgtag agaatgcttt accgagtgaa
    10021 aaaattctca tgcgtgtcct caaggtcaat aaaaagattg gctttggaaa agttgaaaaa
    10081 taccttgtcc agtcaccaca ccgtaatcaa gatctagatt tggcttacct gcgttcagga
    10141 atcgcggatt taggacacct ttcttatcca gaacagctca agtttaaaac caagcaagtc
    10201 aaggacagtc tctacaagat tgctggaatt gcagatgtag aagttgctga aacgcttggt
    10261 atggaacatc cagtcaagta tcgcaataag gcgcaggtgc ccgttcgtcg agtgaatggt
    10321 gtcttggaaa caggattttt ccgtaagaat tcgcataacc tcatgcccct tgaagatttc
    10381 tttatccagg atcctgtcat tgaccaagtc gtagtagctc ttcgagacct gctccgtcgt
    10441 tttgatttaa aaccttatga cgaaaaggaa cagtctggat tgattcggaa tcttgtggtg
    10501 cgtcgtggtc actattcagg acaaatcatg gtcgttttgg tgacaactcg tccaaaagtt
    10561 tttcgtgttg accaattgat tgaacaagtt atcaagcagt tcccagagat tgtgtctgtc
    10621 atgcaaaata tcaacgacca gaataccaat gcgatttttg gtaaggagtg gcgcactctt
    10681 tatggtcaag actatattac ggaccagatg ttgggaaatg acttccaaat cgctggccca
    10741 gccttttacc aagtcaatac tgaaatggcg gagaaactct atcaaacagc cattgacttt
    10801 gcagagttaa aaaaagatga tgtgattatt gatgcctatt ctggtattgg aaccattggt
    10861 ttatcagtcg ccaagcatgt caaagaagtc tacggtgttg aactgattcc agaagcagta
    10921 gagaatagcc agaagaatgc ttctttgaac aagattacta atgcccacta tgtctgtgac
    10981 acggctgaaa atgccatgaa gaaatggctc aaggaaggta ttcaaccaac cgttatcttg
    11041 gttgatcctc cacgcaaggg cttgacagaa agctttatca aagcaagcgc ccaaacagga
    11101 gccgatcgca tcgcctatat ctcctgcaat gtcgcaacca tggcgcgtga tattaaacta
    11161 taccaagagt tgggatatga attgaagaaa gtccagccgg tggatctatt tcctcaaacg
    11221 catcacgtcg agacggtagc acttttgtcc aaactcgatg tcgataagca cataagtgtt
    11281 gaaattgagc tggatgagat ggatttgaca agtgcggaga gcaaagcaac atatgctcaa
    11341 atcaaagaat atgtttggaa taaatttgaa ttaaaagttt cgacattata tattgcacag
    11401 ataaaaaaga aatgtggaat agaattacga gaacattaca acaagtctaa aaaggataaa
    11461 caaattattc cacagtgtac acctgaaaaa gaagaagcca tcatggatgc tttgagacac
    11521 ttcaaaatga tttaatagaa aagaatgaca gtatatgact ttctgcattt attacattcc
    11581 tacttggtat aggaacagct attattcctt tcttgcaagg tatcaattag aaaataggct
    11641 caatataaag attgatagga tcatttttat atttaaagga gcgttgaaat gattgataaa
    11701 ggcaacaaaa aattttagga taaatttgct aagttgtatg cctcttttat gaaaaaagat
    11761 aaagaggttt atgataaagt ttgtgaatat cttagtcctc atttgaataa agatatggag
    11821 gtgcttgaac ttgcttgttg gtttcgtgtc ataacagtta tagaggcaaa tagttatgta
    11881 aatataagga gttcaagact tctaccaaag tttaaaactc aaaaaataaa
//
LOCUS       DAVKNL010000054        11725 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVKNL010000054 DAVKNL010000000
VERSION     DAVKNL010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11725
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.054"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            51..260
                     /locus_tag="VW411_001629"
     CDS             51..260
                     /locus_tag="VW411_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEU9794328.1"
                     /translation="MKIHCLKLKNKELNKEVAFYLTSIIRQALKNTEYKDQISSTVLP
                     DIKIKLPIDSRGTPDWNYMERYRDR"
     gene            226..540
                     /locus_tag="VW411_001630"
     CDS             226..540
                     /locus_tag="VW411_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794329.1"
                     /translation="MGITWKDIEIDKEREGEIDKKSTPIFYGEFKNVRLSKEEYKNLK
                     EKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEEDKDKLRQKGLNKKMNYDVGE
                     SL"
     gene            1224..1385
                     /locus_tag="VW411_001631"
     CDS             1224..1385
                     /locus_tag="VW411_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794330.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            1477..3156
                     /locus_tag="VW411_001632"
     CDS             1477..3156
                     /locus_tag="VW411_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU9794331.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSDKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLHIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENISIQTMEGRIQKAGEGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKD
                     GTKYKDFYYYGCNHRQMIRGNKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEK
                     INMKVDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYR
                     MYDKLDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISAL
                     ISEIQVYEEKQSNGQWLKSITFKLPIIEENLNIGLDNDEQVECVSLLEKRS"
     gene            3424..3990
                     /locus_tag="VW411_001633"
     CDS             3424..3990
                     /locus_tag="VW411_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794332.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            4312..4500
                     /locus_tag="VW411_001634"
     CDS             4312..4500
                     /locus_tag="VW411_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEU9794333.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            4562..5320
                     /locus_tag="VW411_001635"
     CDS             4562..5320
                     /locus_tag="VW411_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU9794334.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVHDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVIF"
     gene            5378..6232
                     /locus_tag="VW411_001636"
     CDS             5378..6232
                     /locus_tag="VW411_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEU9794335.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGIWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCSIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            6344..7228
                     /locus_tag="VW411_001637"
     CDS             6344..7228
                     /locus_tag="VW411_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEU9794336.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            7391..7753
                     /locus_tag="VW411_001638"
     CDS             7391..7753
                     /locus_tag="VW411_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HEU9794337.1"
                     /translation="MELSAIYHRPESEYDYLYKDKKLHIRIRTKKGDIESINLHYGDP
                     FIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTEGQNILYGDKGC
                     VENSLENLHAIGNGFKLP"
     gene            7734..9130
                     /locus_tag="VW411_001639"
                     /pseudo
     CDS             7734..9130
                     /locus_tag="VW411_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
     gene            9216..9533
                     /locus_tag="VW411_001640"
     CDS             9216..9533
                     /locus_tag="VW411_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794338.1"
                     /translation="MNCRGHETRQRIVRDFEVQPKAHIKLLANQQKHSDAGATIEDEY
                     YVFIAESKIDGKKEVIQCCMGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGS
                     GNL"
     gene            9579..9860
                     /locus_tag="VW411_001641"
     CDS             9579..9860
                     /locus_tag="VW411_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794339.1"
                     /translation="MWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKRVNTTIKN
                     GSKGKTLTEMINGYRADNDIRDEICNFNILKNKIRDMKNQQGNTMESYF"
     gene            10305..10781
                     /locus_tag="VW411_001642"
     CDS             10305..10781
                     /locus_tag="VW411_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9794340.1"
                     /translation="MIGKNIKSLRKTHDLTQLEFARIVGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA"
     gene            10781..11542
                     /locus_tag="VW411_001643"
     CDS             10781..11542
                     /locus_tag="VW411_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PezT"
                     /protein_id="HEU9794341.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFAGKMVESLV
                     TKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYIINPNQARATPKEHHDFIVNHLVDNTRKLEELAIFERIQIYQRDRSCVYDSKENTT
                     SAADVLQELLFGEWSQVEKEMLQVGEKRLNELLEK"
BASE COUNT         4231 a         1613 c         2409 g         3472 t
ORIGIN      
        1 tttcattttt aggagcagtc ttttatcata aatataaagc aagcctagat atgaaaattc
       61 attgtttaaa attaaaaaat aaagaattga ataaagaagt agcattctat ttaacttcaa
      121 ttattaggca agctttaaaa aatactgaat ataaagatca aatatctagc acagtattgc
      181 cagatatcaa aattaaatta ccgatagaca gtagaggtac tccagattgg aattacatgg
      241 aaagatatag agatagataa agagagagag ggagagatag ataaaaagtc cacccccatt
      301 ttttatggag aattcaaaaa tgtaaggtta agcaaagaag aatataaaaa tctaaaagaa
      361 aaattaaact cacacacaga tataatgata aataaactat ccagatacat ggaaagcagt
      421 ggtaagacct atcaaaacca ctatgtgaca atcttaaaat ggtatgaaga agataaagac
      481 aaactaagac agaaaggttt aaataaaaaa atgaattatg atgtaggaga atctttatag
      541 ataaaaagag gtggaaaggc actattaaag actttaagtc taaaaaggta tcaaagtatg
      601 ccagagataa aaaaatataa atatgacact ttaaaggcga ttagaaaatt aaagaaatgc
      661 tagaagactt ttagatgaca gttaccaaga gggtctaatt ttcactgcta tttatgcagt
      721 gggaattgga cctttttata taagcaaatt ctatttcaaa attggttacc aagaaagact
      781 tcttttcact gttaagtata agagggtaat ttatagaatt ttgttacatc aagataaata
      841 ttttctgatg gtcaaatttt aaaaagatta ataactctcg ataaattttg aaccttaaca
      901 attgaataga ccaagacaag acgttaatta tttttggagc gtaataacct atccgtcaag
      961 atctttcggc tgaataattc ggcaaaaaaa gttctgctaa gcaaaaagaa agtgagcgat
     1021 aagtaagagt ttgaatatag ggagggatac cctatccgct atgaagaaaa tgagggtaat
     1081 gatacttcta aggttgaagc cggctcatcc ggaatagagg cggaagtgag attcctatgt
     1141 tgctaatcca aagcacttgt caatgtcaaa aaacttaatt aaacaaataa ttattagata
     1201 taaaacttta gaggaggtat atattggaca atgactggaa tggattggct gatttaatag
     1261 caaatctgat tgcaaaatat gctggtgctt tagatttaga taatctaccc gatccaacgc
     1321 cagcaaaaaa tcaagagatg aaaaatagtt ttgacatggc aaaaacacaa attgagacgg
     1381 actaataaaa gtgttataat atacttgaaa tgatagtcca aacttaaatt aaaaaaatta
     1441 tatatgatat agaaaaatta tattgaggtt aatactatgt caaaagaaaa aataaaagta
     1501 tacctctata cacgagtatc tacgtcaata cagatagagg ggtattcgtt agaggcacaa
     1561 aaatcacgaa tgaaagcttt tgctatttac aacgattatg aaattgttgg agagtatgaa
     1621 gatgccggga agtctgacaa gtctattgaa ggtagaatac agttcaatcg aatgatggaa
     1681 gatataaaat ctggaaaaga tggagtatct tttgttcttg tgtttaagtt atcaagattt
     1741 gcaagaaatg ccgctgatgt tctttctaca cttcatataa tgcaggatta tggagttaat
     1801 cttatctgcg ttgaagatgg cattgattca tctaaagatg ctgggaaact aatgatttct
     1861 gttttatcag ctgtggctga aatagaaaga gaaaacatca gtattcaaac aatggaaggt
     1921 cgcattcaaa aagcagggga aggaaaatgg aatggtggct ttgctccgta tggatataaa
     1981 cttgaagacg gcaagctgtt tataaatgag gaagaggcag ttgcaataag aacgattttc
     2041 gaccagtatg taaatactac gataggagcc aatgggatat ctaaatactt agagaatcat
     2101 ggaattagaa aaatcccaag acagaatggt aagaatcctt tgtttgatgc aggtcttata
     2161 agaaagatat taaagaatcc tgtatataat gggaaaatag catttggaag aagaacttta
     2221 gaaaaagttc atggtacaag aaatgaatat aagcaggttg aacaagatga atatctaata
     2281 tctgaaggga tacatgaagc tatagtttcc gatgaagttt ggcaagctgc tcaggttaag
     2341 ctaaaatctc aagcaaagaa atatgagcat gtgaataaag gaaaagatac acgcacacac
     2401 ttgctttctg gaattgtaaa atgcccgata tgtggagtgg gaatgtttgg gaacaagtgt
     2461 atcaagaaaa agaaagatgg cacaaagtat aaagattttt attactatgg ttgtaaccat
     2521 aggcagatga taagaggtaa taagtgtaca ttcagtaagc aaattagaga agaattgtta
     2581 gatgatgctg ttgcagaggt gattgtcaag atagtaagta atccgaaatt tgcttctatg
     2641 atgcaagaaa aaatcaacat gaaggtggat acctctgaaa tagaaaaaga aatagataat
     2701 taccaaaagg aattgagaaa gagtcattct acaaagttta agctaattga ggaaatagat
     2761 aatttagatg ttgaagataa gcattataag agaaggaaac aggatttaga tgatagacta
     2821 tatcgtatgt atgacaaact agatgaatta gaatcatcac taattgatgc gaaagcaaag
     2881 aaacagacta ttgaagctga aaagcttaca ggagataaca tatataaggt tctgatctat
     2941 tttgataagc tctataaagt catgaatgat gtagagcgta ggcagttaat ttcagctttg
     3001 atttctgaaa ttcaagttta tgaagaaaaa caatctaacg gacaatggct aaaatccatt
     3061 acttttaagc taccaattat agaagaaaat ctcaacatag gtttggacaa tgatgaacaa
     3121 gttgaatgtg taagtttatt agagaaacgt agttaatcct caaaaggttc cccgaacctt
     3181 ttgagtccca ctgacgcatc acgctgaggc ggtatcactg cttgtacgag ttgaagcatc
     3241 agcaaagtaa aagtagatgt tcagccaatg cgacgaggtg agaccgaagg tcgataaggt
     3301 gtgttctggg tataagactc gtaaagtaag taggaaatat gatgagatga tacagagagc
     3361 ctgaattggt caggtgacgt gcttatttac attagcctga taatgaaaat ggagagatta
     3421 tcgatggtta tattggaatt gtaccaaaat gattattcta aagatttagt agcatttgat
     3481 tccatagaag atgggaaagc ctttgtagct caaatcccgg gatacactct ggaaacagaa
     3541 gatggttttg aagtggagta ttttaatcct aaaaacatac cggattatat ggaaattatt
     3601 tttaacggaa atattgttcc tttatctaaa tttatgttcg atcctgagga aaatgtaaac
     3661 attatttgga aagagatttc aaatttatcc ctcaaaaatg atagagtgat cgaaggatat
     3721 tcaaaaattg atgcatatgt cgttaacaac catgaagtta aagcctatgt cgaaacaaga
     3781 gaaacgaact atcgtaaagc aaaagacttc ttagaaagcc gcggatatga aatcgacaga
     3841 agctttttcg gaagtgaaga cggtgaagcg attctttaca gaaaaaaagg gatcgaagtt
     3901 tggcatttct tgtgtcactt agacccaatg tttgtagaga ttgaagatgt agaaggatat
     3961 gttaaagaag aagttggaga gattcaatag tcaaccaaag ttccttgtga gtatatacct
     4021 ttgctagtaa aagagtcaaa acgttataac gaaggggggg aagaaaaccc ttgagtgtaa
     4081 gtctttttct tgtttagagg gaaagtatta gagtagccgc tcataattta tactagattt
     4141 gataactgat ttgagacaga ttttatttct ataaatattg tgaaaatttt ttaataccaa
     4201 agatttaaag ttactgtgtt aaaaatcctc tccactttta aactaatgta ttataaaagc
     4261 cagttgacac tagtgttaca cctgtgatag aattaacttg aggtgatgaa aatggcaacc
     4321 gataaaaata gaataatgat tagcttggat gataaaaatc tagagaaact tgaaaattta
     4381 gtagaagatg ctagagatag aaggggaatg cgactaacaa agtcccaggt tattgaatta
     4441 ctcctaaata ccgttgatta tttcgatgat attatggggg ctatttactc aaaaaaataa
     4501 gagcaaaatt gctattattt tttacaaatt ggtgtaacac tagtgatata ggagtgatga
     4561 aatggttgtt tatattagac aaagtaaatt accaagtgag gtttcaatta ataaatacaa
     4621 tgctcaagta ggtgcctact tacaaggaga agaagttatt ttatatcaat ctttttctga
     4681 aataaaagag ttaacaagtg aagatatagt tgtagattat ataatggaga ctagagcatt
     4741 actaaaaatg atgggcttaa acgttccggt tcatgactat cctattgagc ttaaagagtt
     4801 ttatggtcga aagatttatg ctggtatttt aggagagatt gtgaatatac ctgataattg
     4861 gggaaaattt attaagccta aagctggttc aaaagtcttt actggaagag ttgttaatgg
     4921 aacccatgat ttaataggta ttggtctacc tttcgactat cctatatgga ttagtgaggt
     4981 tgtagaattc atagctgaat ggcgttgttt tgtgttagat ggtcgcgtat tagatgttcg
     5041 accctataca ggtgattatc atgcacaatt tgatgcaagt gtaattgatg aagcgatatc
     5101 atgttggaaa gatgcgccaa tagcttatgg actagatatc ggtgttactc gcgatggcag
     5161 aacacttgtt gttgaagtaa atgatggtta tgcattggga aattatggcc tatctccttt
     5221 aaaatcaatc aattttcata gagctaggtg gaaagaaatg gtaaaaccct attttgaaaa
     5281 aaatgaaatt tttaaaatcc aacaggatgt tattttctaa aataaatatg gtagataacc
     5341 aatataaaaa gcatcaaaat gaacaaggag gaatctgatg cttggagcaa ttattggaga
     5401 tattgtcggt tctgtttacg aatggaacaa tatcaaaaca aaggactttc ctttatttcg
     5461 gaaggactgc ttttttacag atgacacggt tatgacctgt gctgttgcag aagcaattat
     5521 gaatggtgga caaaaagatg acttcattga cgcgatgaag aaatatggca gaatgtatcc
     5581 gaatgctgat tacggtgctc ggtttaatgc atggctaaac agcgataacc gtgagcctta
     5641 taatagcttt gggaatggat cagctatgcg tatttctcca tgtgcttgga tcatggactg
     5701 tggtttttat gcgaaaactg gtatatggcc atcatctaga ggacttacga gtctttctgc
     5761 agaggtaact cataatcatc cagaaggtgt caaaggcgct atggctacag ctgatgctat
     5821 ctttctatgt cgtttttact ttggtggtta ttgtagagag tacgagcaat caattaacga
     5881 caatcctaca gagtgtaaaa gacgaattaa ggattatata gaaaaagaat acgattacaa
     5941 tctatctcaa actttagatg aaatccgtcc taactatcgt tttaacgaaa catgtcagga
     6001 aactgtccct caagccatta tcgcctttct tgaaagtaga gacttcgaag atgcgataag
     6061 aaatgccatc tcacttggtg gcgacagtga tactctcgca gcaatcactt gtagcatagc
     6121 agaggcagct tacggtattc ctgattggat caaggataag gcctattctt acttggatga
     6181 acccttaaag gatgtagtta ggcgatggga aaatagaata aaagcgtatt aatctccagt
     6241 acgtcagatt tctcttttct gtattttcat tgtataatag gattacattt acttgcatac
     6301 gacgaaatgt aaacatttct gtttaaaatt aaggataaaa tcaatgaaaa aagcaatggt
     6361 aattatcaac cctacttctg gtggcgagaa ggctttggat tacaaagaaa agctggagaa
     6421 taaagcaaaa gaatactttg aatatgttga aaccaaaatt accgaaaaag cgctggatgc
     6481 aacacatttt gctgaagaag cttctcgtga gcagtatgat gcagtggttg tgtttggtgg
     6541 agatggaact gtcaatgaag tcatttcagg tattgatgag agagactaca ttcctaagtt
     6601 agggattatc ccaggcggta cgggtaacct cattacaaaa cttttggaaa tcaatcaaga
     6661 catcgatggc gcaattgacg aactggattt tgatttaacc aataagattg atatcggtaa
     6721 agcaaatgac aactattttg gttatatctt tagtatcggt tctctgcctg aggcgattca
     6781 caatgttgaa atagaggaca aaacaaaatt cggtatttta acctatgctg taaataccat
     6841 gaagtctgtc atgacagatc aggtctttaa cattaaggtt gagacagaaa atggaaatta
     6901 tgttggtgaa gctagccatg ttttggtcct tttgacaaat tacttcgctg ataagaaaat
     6961 ctttgaagaa aacaaggacg gctatgccaa cattttgatt ctgaaagatg cctctatatt
     7021 ctccaaatta tccgtcattc ctgatttatt aaaaggggat gttgtcgcaa atgataatat
     7081 cgagtatatc aaagcgcgta atattaaaat ctcttcagat agtgaattgg agtcagatgt
     7141 tgacggagat aaatcagata acctacctgt agaaatcaaa gtcctagctc agcgagtaga
     7201 agtattttca aaaccgaaag aggattagta tatagagaaa gcctttttta aggctttttg
     7261 tatactttaa aagatagttc ctttaacaac ggacattcct tgcaaatagt ttacaaaaat
     7321 agtatactgg attcattgag tttgaaaacg tttgcgtaaa atttgaatga atactttagg
     7381 agacaaattg atggaattga gtgctattta ccataggcct gagtcggagt atgactatct
     7441 ttataaggat aagaaactcc atattcgaat tcgaactaag aaaggggaca ttgaaagcat
     7501 caacttgcac tatggggacc cttttatctt tatggaggag ttttatcagg atacaaaaga
     7561 aatggtcaag ataacttctg gtaccttatt tgaccattgg caggttgaag tgtcagttga
     7621 ctttgcacgt atccagtatc tctttgagct cagagataca gaaggtcaaa atattttgta
     7681 tggcgataaa gggtgtgtgg aaaattctct agaaaatctt catgcaattg ggaatggatt
     7741 taagttgcct tagcttcatg agattgatgc ctgcaaggtt cctgactggg tttcaaatac
     7801 ggtatggtat cagatatttc ctgaaagatt tgccaatggc aatgctctat taaacccaga
     7861 agggacttta gactgggatt catctgtcac acctaagagc gatgatttct ttggtggtga
     7921 tttacagggg attattgatc atatgaatta cttgcaagac ttgggtatta ctggactata
     7981 tctttgtccc atctttgaat ctacaagcaa tcacaagtac aatacgacag attactttga
     8041 aattgaccgt cattttggag acaaggagac ctttcgggaa ctggtggatc aagcgcatca
     8101 tcgtggcatg aaagtcatgc tggatgcggt atttaatcat attggttcgc aatctcttca
     8161 atggaaaaat gtcgtcaaaa atggtgaaca gtctgcttat aaggattggt tccatattca
     8221 acaattccca gtgacaactg aaaagctagt taataagaga gacttaccct atcatgtttt
     8281 tggtttcgag gactatatgc ctaagctaaa tacagccaat ccagaggtca agaattatct
     8341 tttaaaggtt gcgacttatt ggattgaaga gtttaatatc gatgcttggc gtttggatgt
     8401 ggctaatgag attgaccatc agttctggaa ggattttcgt aaggcagttt tagctaaaaa
     8461 tcctgatctt tatatcctag gagaagtctg gcatacatct cagccttggc taaatggaga
     8521 tgagttccat gccgtcatga attatccttt atctgatagt atcaaggact atttcttacg
     8581 aggaattaag aagacagacc agttcatcga tgaaatcaat ggagagtcta tgtattacaa
     8641 gcagcagatt tcagaggtca tgtttaatct cttggattca catgatacag agcgaatcct
     8701 gtggacggcc aatgaagatg ttcaactggt taaatcagcc ttagcctttc tctttttaca
     8761 aaaaggaaca ccgtgcattt attacggaac cgagctagcc ttgactggag gaccagatcc
     8821 agattgtcgt cgttgtatgc cttgggaacg tgtatcaagt gacaatgata tgctgaactt
     8881 tatgaagagg ctgattaaaa ttcggaaata cgcgtcagta atcatttcgc atggcaagta
     8941 tagccttcaa gaaatcaact ctgatctagt agctctggaa tggaaatacg aaggacggat
     9001 cctcaaagca atattcaacc aatcaacaga agattatctt ttagagaaag aagcagtagc
     9061 actagcaagc aattgccaag aattggataa tcagcttgtc atctctccag atggatttat
     9121 gattttctaa aaactagttg atgaagatta tggtacattt cataccttat atagtataat
     9181 aaggctagtt actaaacttg taaaggagaa cttaaatgaa ttgtagagga catgaaacaa
     9241 gacaaagaat tgttagagat tttgaagttc agcctaaagc acatattaag ctgttagcaa
     9301 atcaacaaaa acatagtgat gcaggagcaa ctattgaaga tgaatattat gtatttatcg
     9361 ctgagagtaa aattgatggc aagaaggaag ttattcagtg ttgcatgggt gcggcaaggg
     9421 attttttaga actaattaat cacaaagggc tacctctttt taatccgctt gtaggtgatt
     9481 ctcatgtaaa taatagacaa gaatatgaca atacagggag tggaaattta taacctgaaa
     9541 agtggaatga aactgcaaag cagctttata atgctataat gtggttgatt attttatgga
     9601 atgctaagcc ggatacacct ttatttaatt ttaaagacga agtaattaag tataaaacat
     9661 atgagccttt tgaaagcagt ataaaaagag taaatactac tataaagaat ggtagtaaag
     9721 ggaaaactct gactgagatg attaatggct acagagcgga taacgatatt agagatgaaa
     9781 tttgtaactt taatattctg aaaaataaaa ttcgtgatat gaaaaaccaa caaggaaata
     9841 caatggaatc ttacttttag ttattgttga attttgggta ttctataaaa tatcctaatt
     9901 gagatttaaa tagtagacta tacaatatag ttaaaatatc agtaaaaaca acactttatt
     9961 gaggtattgg atacgctttg ctaatagcct aataatcaca tgtggagtgt tgctacaacg
    10021 aaaaaggtga taatccttga tttcaagcta ttttataagc attttgtctt tgtagataaa
    10081 ggcaattttg acaataaaaa tcctaaaagg tgaatcgtta tagatgtatt tgtagatatc
    10141 gtttgcgcat cgaaaaaatt aatacaagaa taaatattta tagctcttta ggtgactttt
    10201 atagaagtaa agtttaggat agaaaaacaa gaaataacgc accatttttg gtgcgttatg
    10261 cttttttatg ctataatgga tttataaaaa taaaggagtt tgctatgatt ggaaagaaca
    10321 taaaatcctt gcgtaaaaca catgacttaa cacaactcga atttgcacgg attgtaggta
    10381 tttcacgaaa tagtctgagt cgttatgaaa atggaacgag ttcagtctct accgaattaa
    10441 tagacatcat ttgtcagaag tttaatgtat cttatgtcga tattgtagga gaagataaaa
    10501 tgctcaatcc tgttgaagat tatgaattga ctttaaaaat tgaaattgtg aaagaaagag
    10561 gtgctaatct attatctcga ctctatcgtt atcaagatag tcagggaatt agcattgatg
    10621 atgagtctaa tccttggatt ttaatgagtg atgatctatc tgatttgatt catacgaata
    10681 tctatctagt agaaactttt gatgaaatag agagatatag tggctatttg gatggaattg
    10741 aacgtatgtt agagatatct gaaaaacgga tggtggccta atggaaatcc aagattatac
    10801 tgatagtgaa ttcaaacatg ctttagcaag gaatcttcgt tcactgacaa gaggaaaaaa
    10861 gtccagtaag caacctatag cgattttgct tggagggcaa agtggtgccg gtaagactac
    10921 aattcatcgt attaaacaga aagaatttca aggaaatatt gttatcatag atggtgatag
    10981 ttttcgttct cagcatccac actatttaga actgcagcaa gaatatggca aagacagtgt
    11041 agaatatacc aaagattttg caggaaaaat ggtagagtct ttagtaacaa aattgagtag
    11101 tttgagatac aatcttttga tagagggaac tttacgaaca gttgatgttc caaagaaaac
    11161 agcacaactc ttgaaaaata agggatatga agtacaattg gccttaattg cgacaaagcc
    11221 tgaattgtcg tatctaagta ctcttatccg ttatgaagaa ctgtacatta tcaatccaaa
    11281 tcaagcacgc gcaactccaa aagaacatca tgatttcatt gtaaatcatc tagttgataa
    11341 cacacgaaaa ttggaagaac tagctatctt tgaaagaatt caaatttacc aacgagatag
    11401 aagttgtgta tatgattcaa aagaaaatac aacttcagca gcagatgttc ttcaagagtt
    11461 actctttggg gagtggagtc aggtagagaa ggagatgttg caggtggggg aaaagagact
    11521 taatgaatta cttgaaaaat aaacaattga tatttttagg agaatagaaa tgagagggtt
    11581 taataacaag ataaagtctg tttatcaaga actaacaaat tccaaagaga aattcggtag
    11641 ctttcacaag actttaattc atttgcatac acctgtttct tatgattaca agctattttc
    11701 taattggact gcaacgaaat ataga
//
LOCUS       DAVKNL010000055        11669 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVKNL010000055 DAVKNL010000000
VERSION     DAVKNL010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11669
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.055"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            1..1070
                     /locus_tag="VW411_001644"
                     /pseudo
     CDS             1..1070
                     /locus_tag="VW411_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1195..1398)
                     /locus_tag="VW411_001645"
     CDS             complement(1195..1398)
                     /locus_tag="VW411_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9794342.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(1391..2473)
                     /locus_tag="VW411_001646"
     CDS             complement(1391..2473)
                     /locus_tag="VW411_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU9794343.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFNLFTSTSKDLLYFD"
     gene            complement(2599..3381)
                     /locus_tag="VW411_001647"
     CDS             complement(2599..3381)
                     /locus_tag="VW411_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEU9794344.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(3381..4724)
                     /locus_tag="VW411_001648"
     CDS             complement(3381..4724)
                     /locus_tag="VW411_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEU9794345.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(4823..6139)
                     /locus_tag="VW411_001649"
     CDS             complement(4823..6139)
                     /locus_tag="VW411_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEU9794346.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQSLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            6332..7558
                     /locus_tag="VW411_001650"
     CDS             6332..7558
                     /locus_tag="VW411_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEU9794347.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GLVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKALT"
     gene            7757..9331
                     /locus_tag="VW411_001651"
     CDS             7757..9331
                     /locus_tag="VW411_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9794348.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
     gene            9595..10383
                     /gene="codY"
                     /locus_tag="VW411_001652"
     CDS             9595..10383
                     /gene="codY"
                     /locus_tag="VW411_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEU9794349.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PIESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            10383..10958
                     /locus_tag="VW411_001653"
     CDS             10383..10958
                     /locus_tag="VW411_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU9794350.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELFE"
     gene            11040..11125
                     /locus_tag="VW411_001654"
     tRNA            11040..11125
                     /locus_tag="VW411_001654"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11074..11076,aa:Leu,seq:aag)
     gene            complement(11248..>11669)
                     /locus_tag="VW411_001655"
     CDS             complement(11248..>11669)
                     /locus_tag="VW411_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9794351.1"
                     /translation="SDKRFYRPTFRMHLTNKEILDKILSYSEDLKHHYQIYQLLLFHF
                     QNKDPEKFFGLIEDNLKQVHPLFQTVFKTFLKNKEKIVNALQLPYSNAKLEATNNLIK
                     LIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSQA"
BASE COUNT         3458 a         2170 c         2570 g         3471 t
ORIGIN      
        1 ataaagtctg acatgaaggc tggactaaag atagagtttc tcttgaatac ggtctcccaa
       61 gtcgtacgat acttcttaac tggctagcac aatacaggaa aaacgggtat actattgttg
      121 agaaaacaag agggagagta cctgagagcg gagaatgcca tcctaaaaaa gttaagagaa
      181 ctccgattga aggaggaaaa agagaaagaa gaaagacaga aattgttcaa gaattaatga
      241 ctgagttttc gttagatctt cttctagaag ccattaaact agctcgttgg acctactact
      301 atcacttgaa acagctagac aaaacagata aagaccaaga gcttaaaact gaaattcaat
      361 ccatctttat cgaacacaag ggaaattatg cttatcgccg ggttcattta gaactaagaa
      421 atcgtggtta tctggtaaat cataaaagag ttcaaggctt gatgaaagta ctcaatttac
      481 aagctaaaat gcgaaagaaa cgaaaatatt cttctcataa aggagacgtt ggtaagaagg
      541 cagagaatct cattcaagcc caatttgaag gctctaaaac aatggaaaag tgctacacag
      601 atgtgactga atttgccatt ccagcaagta ctcaaaagct ttacttatca ccagttttag
      661 atggctttaa cagcgaaatt attgctttta atctttcttg ttcgcctaat ttagaacaag
      721 tacaaaccat gttggaacag gcattcacag agaagcacta cgagaatacg attctccata
      781 gtgaccaagg ctggcaatac caacacgatt cttatcatcg gttcctagag aataagggaa
      841 ttcaagcatc tatgtcacgc aagggaaaca gccaagacaa cggtatgatg gaatctttct
      901 ttggcatttt aaaatccgaa atgttttatg gctatgagaa aacatttaaa tcacttaacc
      961 aattggaaca agtcattata gactatattg attactacaa caacaaacga attaaggtaa
     1021 aactaaaagg acttagtcct gtgcagtaca gaactaaatc cttcggataa attaattgtc
     1081 taactgtttg gggtcagtac acaatacctg atactatgcg ttttattgtg gggagatttt
     1141 cctcattttc tagtgaaatt gaactttttc taacatcttt tgatagatga cttcttaatc
     1201 aattatcatt tccattgttg tttcttgggg tttcatccct tgtctttgat aaaagcggag
     1261 agcaccctcg ttatcattcc agacacttag ggttagatta ttgcagccaa tttctttagc
     1321 ataatagcag aaaaatgaca ataaaatagc ccctagatat tttgaaagga aagcataggg
     1381 gctatatagt ttaatcaaaa taaagcaaat ctttgctggt gcttgtaaag aggttgaagc
     1441 catccttggt aacaacaccg cagtcttcaa tacgaacacc gactttacca gggatataga
     1501 tacctggttc aacagagaag cacatgcctt cttcgatgac catgtcgttt ccttccatga
     1561 tagatgggaa ttcatggaca tccataccga taccatgccc gagacggtgg ttgaagtact
     1621 caccataacc agctttttcg atgacctcac gggcagcgcg gtccacttca tgagcagtca
     1681 cacctggctt gataaagtca agagcagctt gttgggcttc aagagtcaag ttgtaaatat
     1741 ctttcttgaa ttggtctggt ttgccgacag cgactgtacg agtcatatct gacgcatagc
     1801 cattgaccag aacacccagg tcaaagagga gaagagcatc attttcaacc ttattagctg
     1861 ctggaatgcc gtgtggattc gcagcattat caccagtcaa gaccatggta tcaaagctca
     1921 tttcataacc ttcacgtttc atggcaaagt cgatttgtgc gatgatatct gtctcagtct
     1981 tatcaagaga aatattgtca aaaccaacat gaacagcctt gtcagcataa agacctgcaa
     2041 ccatcatttt ttgcacttca tcagctgatt tgatgaggcg catgcgttgg atacgaggag
     2101 tgaggttgtc aaactcagca gtctcaaaaa ctgttttcaa accatggtat ttggtcaaga
     2161 tgagattgtc aaactcaaca gcgacacgtt tgaagtcaag ttgtggaaga gcatgtttga
     2221 ttttttgcca tggattttca gaatcgacat agcccactac tgggaaggaa acggtgctac
     2281 ttgcacgttc tacttcaaga gctgggacaa agaggagagg ttcctgatct gctaggacaa
     2341 agaggaacat ttggcgttca tggggatcac tgtaaaaacc agtgaggtaa ttgattgtga
     2401 cggggtcaga tacgacagcg acgtctagtt tttctgattc aagatatgtt aggatttgtt
     2461 gtaatttaga catatgctac cttctttcta cccctctatt ttggcaaaaa atgagagaaa
     2521 atgcaaggga gaagggtaaa agaaaagaca aaaaactccg acaagataac ggagttcttt
     2581 gatatcattt tcctttgttt aagaaaagtc agccttgctt ttgacgtcac tgtactcttc
     2641 agcgatttct tggctgagaa tgtcctcata ggcagggagt tggatgtcct gaccatattt
     2701 cttcttgagg gcagtagtaa ctaggcgata agccagattg gcattacgag agaggatagg
     2761 cccgtggaag taggaaccaa agacattctt ataatgaacc ccttcaccga ccttttcttc
     2821 gttgtttcca tttccataga caacctgtcc cagcggtttt tggtcatcag agaggaaggt
     2881 acgaccttgg tgattttcaa atccatagta ggtttcatcg aaatcttcat tgtgaatctt
     2941 gatgtcaccg ataaaacggt tattggtctg gttgagcgtg tagtgtccca tgacccctag
     3001 cccttcgata cgttttcctg aagcttcaac ataatattga cccaatagtt ggaaaccacc
     3061 gcagatagcc agaactacac cgtcgttttg gatgtagttg tcaatgctct cttttttagc
     3121 aggtaggtcg tctgcaatga tactttgttc aaagtcttga ccaccaccga aaaaggcgat
     3181 gtcgtagtga ttttcatcaa agtcatcatg gagagaaacg atgtcaacgg tcacatgggc
     3241 tcccagtttt tcagccacat acttgagcat gaggatgttt ccattgtccc cgtaggtatt
     3301 catgagattt ccgtagaggt gggcaatgtt gagctgatag gggtaattgc catcttttga
     3361 ggaaagtgaa gtataaacca ttagttcatc tcctttctaa caatctgacg actagccagc
     3421 agttcacgaa attccagcat ggcagtataa gttgccagaa tataggcatg cttgcagtct
     3481 tgattctcaa tggtcttgag aacttgctcc agattactcg tttcagtgat tttctcagct
     3541 ggatagccag tcactcggag gcgacgagcg atttcagaat gacgaacacc gccagcgttg
     3601 atttcaggaa tgtccatgtc agtgatttgt tcaaagtctg catcccagat ccagctagtg
     3661 tcaattccat ctgcatagtt ggcattaagg aggacagata ggctaaatgg ataaggtgct
     3721 agtttgatca tttcgatagc ttgggttgca ccgactggat ttttaatcaa gacaagggta
     3781 cattccttgt caccgatatg aaaggtttct tggcgtccaa agacagcacg gctcttgtca
     3841 aatccctgtt tgatgagttg cgaatcggca cctaggaaac gggcgatggc cacagcagct
     3901 agggcgttat agatattata gagcccgccg atttggatac cgtattcttg gccgtctatg
     3961 acaaagcgag agcgattgtt ggtcaactca accagttttg tcaaacgata gtcgagatca
     4021 ggacgtttac atccacaacc ttcacagata taggcaccca agtttgcata ggtattatgc
     4081 tcatatttga ggatgccttg gcagtcagga cagagaatcc cttcggtatt gtagtgagcc
     4141 agttgggctg gtcccttttc caagtcaaaa ccaaaatact ctatagggtt tggaatagtt
     4201 ggcttgtaga aaagtggact gtctccgtta aggagaacag tagcagttgg aactttccga
     4261 atggcatcca atatcatgtt ataggtagta tagatttcac cgaaacggtc catctggtca
     4321 cggaagatat tagtaatgac aaaaagacta ggctggatat agtcacagat acgagataga
     4381 ctggcttcgt caatttcgag gacggcaata tttttcccag ttttagaaga tttggctgtt
     4441 aggaaggttg ttgcaatccc tgtaatcatg ttggcaccgc ttgggttggt tagaacttga
     4501 ccataaacct cttttaaaat gccgacagtg agggcagttg tcagggtttt tccatttgtt
     4561 ccagtgacaa cgacaatctc gtagttctta gctaggtttt gtaaaatatc tttatcaaat
     4621 tgaagggcga ctttccctgg gagcgtactt ccacgtccaa gacggcttaa aacgaagtgg
     4681 gaagaacgcc cagcaagaag gcccaaagta gtttttaagt tcatgtttct attatatcat
     4741 aaaagaggag aaagacagat ttgagattcc gcagaagtaa aaacagccca aagagggctg
     4801 ttcgatacga ttaaatctca acttaaatcg caaacaagtc attcaagttc tcagccaagg
     4861 ttgggtgagt gaagatttgt tttgtgaagt aagtgtaagg aatcttgttg tccatagcaa
     4921 cagtgatgat gttgatgatt tcttgagaac cttctgagaa gatgcttgct ccaagaattt
     4981 cttttgtttc agtattgaca acagctttga aggcaccgcg aaggtctcca tttacgtgac
     5041 cacgaggcat tgctgcaacg gggatttcct taacagcgta tggaagtttc aaatcagctg
     5101 cttggctttc agtcaaacca acttgtgaaa gtgcaggtgt gatgaacata gtatttggca
     5161 cattgagacg gtcttcaagt gtatagctgc catctccagc aaggtagctg taaacaacac
     5221 ggaagtcatc aagtgaaatg taagtaaatt gaaggccacc gttgacatct ccaactgcaa
     5281 agacaccagg aacgtttgtt tgacagtgtt tgtctacttt aatagcacca cgttcagtta
     5341 gttcaatatc tgtattttca agttgaagtg gttctacatt tggtttgcgt ccagttgcgt
     5401 agagaagggc gtcgaaacgg taagtttcgt cttcagttac gacaagcact tggtcaccat
     5461 cgtttttgat ttcagtagta tggatatttt gaagcaattc aatgccatct tcttccatgt
     5521 attgtttagc aagagctgcg atggaaggtt ctgcacgagg taggaatgta tccaaggcat
     5581 ctaggactgt gaccttgctt ccaagtttgt tgtaaaggcc ggcaaattca agaccgatat
     5641 ttccgccacc aaggattcca agtttttcag gtaatttgtc caagctttgg atacctgttg
     5701 agtcaaagat gtttttgctt gtagcaagtc cagggattgg caagacgttt gaaacagcac
     5761 cagtgttgat gacgattgtt tcagcagtca gttctttctt ttcatcacca gcttggattt
     5821 cgatgacttt atttgaaagg aagtgagctt ccgcatcaaa gatatctacg cctgtaccag
     5881 caacagtcgc atagttttta ccgttgaggc gaccagtgat cgtgttttta gtagcaatga
     5941 cttcttcaaa agacaagtcc ttttcagcag caactagcaa ggttttagtt gggatacaac
     6001 caatgttgat acaagttcca ccgtacatag ccttgctgcg ttcaacgagg gcaacttttt
     6061 tgccagctga agccaattta cctgctagtg ttttaccagc tttaccaaat ccaataacaa
     6121 ttaaatcgta tgttaacatt tatcttcctc ctattcgaat ttcattctag cacaagaaaa
     6181 aaacttttgc acaattctgc tacggaaaag acagaaaaag ataaaaacgc tttccagaca
     6241 aaaaactagt aattttttga caagcttgat aaactaaaat cctatctatt tttatataag
     6301 atagaataaa tgttcgaaaa taggaggttt tatgaaatcg aatcgttata ttattgcctt
     6361 tgctggggtc attttacact taatgctggg ttcgacttat gcttggagtg tttatcgtaa
     6421 ccctattatt gaaaaaacgg gatgggatca ggcttctgtt gccttcgcct ttagtctagc
     6481 aatcttttgt ttgggcttat cggctgcatt tatggggcgt ttggtagaaa aatttggtcc
     6541 gaaagtcatg ggaagtctat ctgcttttct atacgcaggt ggaaatatct taacaggatt
     6601 tgcaatagac cgtcaggagc tgtggttgtt gtatctcgct tatggcattt taggtgggct
     6661 tggtttggga gcaggctata ttacccctgt gtcgacgatt ataaaatggt ttcctgataa
     6721 acgtggtctc gcaacaggtt tagcgattat ggggtttggt tttgcttctt tattgactag
     6781 tcccatagcg caacacctca tcgcaggggt agggcttgta gaaacttttt atattttagg
     6841 agcaagttac tttattatca tgctcctagc ttcacaattc attaagcgtc caaatgagca
     6901 agagcttgca attttatctt cttcagggaa agaaaaaaca gcctctttga cgcaaggaat
     6961 ggctgcaaat caggctctaa aaagcaatcg gttttatatg ctttggatta ttttctttat
     7021 caacatagct tgtggtttag gcttaatttc agcggcatcg ccaatggcac aggagatggc
     7081 tggcttgtct acaagtcatg cagcagtaat ggtgggtgtt ttggggattt tcaatggatt
     7141 tggtcgcttg ctctgggcga gtttgtctga ctatatcggt cgccctctaa cctttagtat
     7201 attactgctt gtcaatcttt tcttttctct ctcactttgg ctctttacag attccgtttt
     7261 atttgtagtt gctatgtcta ttttgatgac ttgctatgga gctggttttt ctttgattcc
     7321 agcttatctc agtgatattt ttggaaccaa ggaattggcc gctctgcatg gttatatttt
     7381 aacagcttgg gcaatggctg gtttagcggg acctatttta ttagcagaga cttataaaat
     7441 ggctcattcg tacacacaaa ccttgttcgt ttttctcatt ttatacagta tcgccttggc
     7501 tttgtcttat tatctaggtc gttcaatcaa aaaagaaagt caaaaagcgc ttacatgatt
     7561 ttctgtagaa atggggctat cttttgctct ggtcttgtgt tatactagat aggttgcaaa
     7621 gaaaacagta cttttctttt gtggaaaaga agcaacacga ttttatatct agttatatga
     7681 aaatacgtca taaaaagaaa agtataaact aagccctata gggtggcttc gcaccacctt
     7741 tagaaagaag aataacgtga aatttaatga attaaacttg tctgctgatt tgctagcaga
     7801 aattgaaaaa gctggttttg tagaagctag tcctatccaa gaacaaacta ttcctttggc
     7861 ccttgaaggc aaggatgtta tcggtcaagc tcagactggt acaggaaaaa ctgcagcctt
     7921 tggcttgcct acccttgaaa aaatccgtac agaagaagcg actatccaag ccttggtcat
     7981 cgctccaact cgtgaactag ctgtccaaag tcaagaagaa ctcttccgct ttggtcgtag
     8041 taagggagtc aaagtccgtt cagtatatgg cggatcaagc attgaaaaac aaattaaggc
     8101 tcttaaatct ggtgcccata ttgtggtggg aactccaggt cgcctcttgg acttgattaa
     8161 acgcaaggcc ttgaaattac aagacattga aacccttatc cttgacgaag cggatgaaat
     8221 gcttaacatg ggcttccttg aagacatcga agccattatt tcccgtgtac ctgagaaccg
     8281 tcaaactttg cttttctcag caactatgcc agatgccatc aaacgtatcg gtgttcagtt
     8341 tatgaaagcc cctgaacatg tcaagattgc ggctaaggaa ttgacaacag aattggttga
     8401 ccagtactat atccgtgtta aggaacaaga aaaatttgac accatgactc gtctcatgga
     8461 tgtggcacaa ccagaactcg ctattgtatt tggtcgtacc aaacgccgtg tggatgaatt
     8521 gactcgtggt ttgaaaattc gtggcttccg tgcagaagga attcatggcg acctagacca
     8581 aaacaaacgt cttcgtgtcc ttcgtgactt taaaaatggc aatcttgatg ttttggttgc
     8641 gacagacgtt gcagcgcgtg gtttggatat ttcaggtgtg acccatgtct acaactacga
     8701 tattccacaa gatcctgaga gttatgttca ccgtatcggt cgtacaggtc gtgctggtaa
     8761 gtcaggtcaa tctattactt ttgttgctcc aaacgaaatg ggttaccttc aaatcattga
     8821 aaacttgact aagaaacgca tgaaaggtct caaacctgca agtgtagaag aatccttcca
     8881 atcaaaaaaa caggtagctc tcaagaaaat cgaacgtgat tttgcagatg aaaccattcg
     8941 tgccaacttt gagaaatttg gtaaggatgc tcgcaaattg gctgctgagt ttactccaga
     9001 agaattggca atgtatatct tgagtctgac agtccaagac ccagatagcc ttccagaagt
     9061 ggagattgca cgtgaaaaac cactaccgtt taaaccatca ggtaatggtt tcggtggtaa
     9121 agctaaggga ggtcgtggag gccgtcgtgg ggacgaccgt cgagagcgtg atcgccgtgg
     9181 caatggtcgc cgtgatgagt tcaaaaaagg aagtcgtggc aacgatcgtt ttgataagga
     9241 aaaacgttac cgtaaggata ataaaaaacc acgcaatact ttaagcgaaa agcaaacagg
     9301 ctttgttatt cgtaacaaag gcgataaata agaaaaagcg gttcaaaatg aatcgctttt
     9361 ttatataagg agaccgaatg tgaaaagaca aataaaatta ttatattatc ttttataata
     9421 ttatgataca caaaaatagg caacttgtca atagaaaagg aacaatttaa ttaaaaatat
     9481 tctggttttt aattaaattg taagtcaatg caagtcaata aggaattttc agataattat
     9541 tgaaaaagag acgtttttat gttataatga tagcgattaa attcgaggtg aaaaatggca
     9601 catttattag aaaaaactag aaaaattaca tcaattttga aacgctcaga ggagcagttg
     9661 caggatgagc tcccttacaa cgctattacg cgtcagttag cggatattat tcattgcaat
     9721 gcctgcatta tcaatagtaa gggacgtctg cttggctatt ttatgcgtta taaaacaaat
     9781 acagatcgcg tagagcaatt cttccaaact aagattttcc cagatgacta cgttcaaggg
     9841 gctaatatga tttacgaaac agaagcaaac ttgcctgttg agcatgatat gagtattttc
     9901 cctattgaga gtagagatga ttttccagat ggcttgacga ctattgcacc gattcatgta
     9961 tcggggattc gccttggttc tttgattatt tggcgtaatg ataaaaaatt cgaagatgag
    10021 gacttggttc ttgttgagat tgccagtacc gttgttggga ttcagcttct taacttccaa
    10081 cgtgaagaag atgagaaaaa tattcgtcgt cgtactgctg tcaccatggc ggtcaatacc
    10141 ctttcttact ccgaactccg tgctgtttca gcaattttag gggaattaaa tggaaatgaa
    10201 gggaagttga ctgcgtcagt gattgcagat cgtatcggaa tcactcgctc tgtgattgtc
    10261 aatgctcttc gtaaacttga gtctgcgggg attattgaaa gtcgctcact tggaatgaag
    10321 ggaacctatc ttaaggtctt gatttcagat atttttgaag aagtgaagaa aagagattac
    10381 taatgacaaa ggctttaatt tcgattgatt atacagaaga ttttgttgct gatagtggga
    10441 aactaacagc aggtgctcca gctcaggcga tttcggatgc catcagcaag gtaactcgat
    10501 tagcttttga acgaggagat tatatcttct ttactattga tgctcatgaa gaaaacgatt
    10561 gtttccatcc ggaaagtaag ttatttcctc ctcataatct gattgggacg agtggacgga
    10621 atttatatgg agatttgggg atcttttatc aagagcatgg ttcagacagt cgtgtctttt
    10681 ggatggataa acgccattac tcagcttttt cagggactga cctagatatt cgtttgagag
    10741 agcgtagagt gtctaccgtt atcttaacag gtgtcttgac ggatatctgt gtcctacata
    10801 cagctataga tgcctataat ctaggatatg acatcgagat tgttaaacca gctgttgctt
    10861 ccatctggcc tgaaaatcat caatttgccc taggtcattt caaaaataca ctcggagcta
    10921 agttagtaga tgaaaatcta aatgaacttt ttgagtaacc tggttggtga aaatgaaaaa
    10981 atttgaaaaa aagtgttgac aagcatccca aaagttgata tactagtaaa gtaatcgacg
    11041 cggggatggc ggaattggca gacgcgcagg actaaggatc ctgtgaccgc tttaggtcgt
    11101 gagggttcaa gtccctctct ccgcatagga aaagagctag ctaaagctag ctcttttgta
    11161 tttctctaaa aaattagaat ccagtcaaat gtagtggaga atcagcgaaa ataggctctt
    11221 tgtcaactgt agtgggttga agaaaagcta agcttgagaa aggacaaatt tcgtcctttc
    11281 ttttttgatg ttcagagcga taaaaatccg ttttttgaag ttttcaaagt ttcgaaaacc
    11341 aaaggcattg cgtttgataa gtttgatgag attattggtc gcttccaatt tggcgtttga
    11401 atagggtagt tgaagggcgt tgacgatttt ctctttgttc tttagaaagg ttttaaagac
    11461 agtctgaaaa agaggatgaa cctgcttcag attgtcctca atgagtccga aaaatttctc
    11521 agggtctttg ttctgaaagt gaaaaagtaa gagttgatag atctgatagt ggtgtttcaa
    11581 gtcttctgaa tagcttaaaa tcttgtcaag aatttcttta tttgttaagt gcatgcgaaa
    11641 agtagggcga taaaaacgtt tatcgctga
//
LOCUS       DAVKNL010000056        11606 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVKNL010000056 DAVKNL010000000
VERSION     DAVKNL010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11606
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.056"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            122..880
                     /locus_tag="VW411_001656"
     CDS             122..880
                     /locus_tag="VW411_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9794352.1"
                     /translation="MALVEFKNVEKYYGDYHAFRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLETVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKAVLENVT
                     LAPIEVLGIDKKEAEKTAQKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLAHDGMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDTTDVDNFFDNPSEPRAQQFLSKIINHESDKVK"
     gene            920..1684
                     /locus_tag="VW411_001657"
     CDS             920..1684
                     /locus_tag="VW411_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9794353.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLKFK
                     FVELGSYPELITSLHAHRIDTFSVDRSILSGYTSKRTALLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            1697..2374
                     /locus_tag="VW411_001658"
     CDS             1697..2374
                     /locus_tag="VW411_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9794354.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAVGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHIIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQAFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            2384..3043
                     /locus_tag="VW411_001659"
     CDS             2384..3043
                     /locus_tag="VW411_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9794355.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVT
                     RNGKNPVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            3301..4491
                     /locus_tag="VW411_001660"
     CDS             3301..4491
                     /locus_tag="VW411_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEU9794356.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHSLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKTSGDHTKNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(4812..5693)
                     /locus_tag="VW411_001661"
     CDS             complement(4812..5693)
                     /locus_tag="VW411_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEU9794357.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(5834..7162)
                     /gene="vncS"
                     /locus_tag="VW411_001662"
     CDS             complement(5834..7162)
                     /gene="vncS"
                     /locus_tag="VW411_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEU9794358.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENSLTFFIDFPKVVQD"
     gene            complement(7159..7815)
                     /gene="vncR"
                     /locus_tag="VW411_001663"
     CDS             complement(7159..7815)
                     /gene="vncR"
                     /locus_tag="VW411_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEU9794359.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(7864..7947)
                     /gene="pep27"
                     /locus_tag="VW411_001664"
     CDS             complement(7864..7947)
                     /gene="pep27"
                     /locus_tag="VW411_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198524447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEU9794360.1"
                     /translation="MRKEFHNVLSSGQLLADKRPARDYNRK"
     gene            complement(8127..9506)
                     /gene="vex3"
                     /locus_tag="VW411_001665"
     CDS             complement(8127..9506)
                     /gene="vex3"
                     /locus_tag="VW411_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEU9794361.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(9558..10205)
                     /gene="vex2"
                     /locus_tag="VW411_001666"
     CDS             complement(9558..10205)
                     /gene="vex2"
                     /locus_tag="VW411_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEU9794362.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQINYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(10218..11495)
                     /locus_tag="VW411_001667"
     CDS             complement(10218..11495)
                     /locus_tag="VW411_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9794363.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNAITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT         3261 a         2605 c         2199 g         3541 t
ORIGIN      
        1 tgtcccgatc gatttttcct gttcttattt cactatagtg aattttttca gatatgattt
       61 gacaagaaca ttcttttatt gtagaaccgt gttataaaat ataacacaat agagatctct
      121 catggcttta gtagaattta aaaacgtcga aaaatattac ggagactacc acgcattccg
      181 caacatcaat ctccgttttg aaaaaggaca agttgttgtc ctgcttggac cttctggctc
      241 tgggaagtcc actcttatcc gtacgatcaa tggtttagag actgttgaca aaggaagtct
      301 cctagtcaat gggcaccaag ttgctggtgc cagccagaaa gatttggtac ctcttcgcaa
      361 ggaagtcggc atggtttttc aacattttaa cctttatcca cacaaagctg tgttagaaaa
      421 cgtaacgctt gcacccattg aagttctagg aattgataaa aaagaagctg aaaaaaccgc
      481 ccaaaaatat ctggaatttg taaatatgtg ggacaagaaa gattcctatc ccgccatgct
      541 atctggtgga caaaaacagc ggatcgccat cgctcgtggt cttgctatgc atccggaact
      601 cctcctcttt gatgaaccaa catctgctct tgatcctgag actatcggag atgttctagc
      661 agttatgcag aaactggcgc atgatgggat gaacatgatc atcgttaccc acgaaatggg
      721 ctttgctcga gaggttgcgg accgcattat ctttatggcc gacggagaag ttttagtaga
      781 tacgacagat gtcgataact tttttgacaa tccaagcgaa cctcgtgccc aacaattcct
      841 cagcaaaatt atcaaccacg aaagtgacaa agtcaaataa ggaggcgcct atgaaaaaaa
      901 attcttttta tcagcattat tgattagcct tttcggcctt gctgctgcca aaccagtcca
      961 ggctgataca agtatcgcag acattcaaaa aagaggcgaa ctggttgtcg gtgtcaaaca
     1021 agacgttccc aattttggtt acaaagatcc caagaccggt acttattctg gtatcgaaac
     1081 cgacttggcc aagatggtag ctgatgaact caaggtcaag attcgctatg tgccggttac
     1141 agcacaaacc cgcggccccc ttctagacaa tgaacaggtc gatatggata tcgcgacctt
     1201 taccatcacg gacgaacgca aaaaactcta caactttacc agtccctact acacagacgc
     1261 ttctggattt ttggtcaata aatctgccaa aatcaaaaag attgaggacc taaacggcaa
     1321 aaccatcgga gtcgcccaag gttctatcac ccaacgcctg attactgaac tgggtaaaaa
     1381 gaaaggtctg aagtttaaat tcgtcgaact tggttcctac ccagaattga ttacttccct
     1441 gcacgctcat cgtatcgata ccttttccgt tgaccgctct attctatctg gctacactag
     1501 taaacggaca gcactactag atgatagttt caagccatct gactacggta ttgttaccaa
     1561 gaaatcaaat acagagctca acgactatct tgataacttg gttactaaat ggagcaagga
     1621 tggtagtttg cagaaacttt atgaccgtta caagctcaaa ccatctagcc atactgcaga
     1681 ttaaggagga caccccatga cagatttatc atcttggaca gcctattttc aggattttgg
     1741 acaatttttc aatggtttcc tcttcaccct tgccctagcg gttggttcct ttatcctcgc
     1801 tatggttttg ggcatcttct ttggtgccat gtcaaccagt aaacgtccaa ttttacgcat
     1861 tttagctcgc atctttgttg aattttacca aaatactccc ctcttggtgc agtttgttat
     1921 tgttttttat ggcctacctc ttatcagtga ccatatcatc atgattccaa tttattggac
     1981 agccgttctc tgcgtgggac tctatcacgg cgcttatatc gctgaggtta ttcgttcagg
     2041 gattcagtct attcctagtg gtcagatgga ggccgccttg tcgcaaggtt ttacctatat
     2101 cagtgccatg cgcttgatta tcttgcctca ggccttccgc atcattctcc ctccattgac
     2161 caaccaaatt gttaacctca tcaagaacac ctctactgta gctatcatct ctggagtaga
     2221 cttgatgttt gtgactaagt cttggtcggc tctcaacgga aactatatac cagccttttt
     2281 aggtgctgct cttctctact ttgctctatg cttccctgtt gcccagtttg gtcgcaagat
     2341 ggagcaagcc aacaaaaaag cctattcact ttaggaggtt actatggaat ctattttaga
     2401 agttttaacc ccagataacc tagtctttat ctttaaagga tttggcttga ccctctatat
     2461 ttctctgatt gccatcatcc tctctactat catcggtacg gtgctagctg tcacgagaaa
     2521 tggcaaaaat cctgtcttac gcattatttc cagtatttat atcgagtttg tgcgcaacgt
     2581 tcccaacctt ctctggattt ttactatctt tttggtgttc aaaatgaaat ccacaccagc
     2641 aggtattaca gcctttactc tctttacatc agcagccttg gctgagatta ttcgaggcgg
     2701 tctcaatgcc gtagacaagg gacagtacga agcaggaatg tcacaaggct tcacctcagc
     2761 ccaaatcctc tactacatca ttctcccaca agccatccgc aaaatgctac cagccatcat
     2821 ttctcagttt gttaccgtga ttaaggatac cagtctcctc tactctgtta tcgccctaca
     2881 agaactcttt ggagccagcc aaattctcat gggccgttat ttcgaaccag agcaggtctt
     2941 cagtctttac atcctgattg ccctcatcta cttcagcttt aacctagcaa tttctagcct
     3001 gtctcatatg ctagccaaac gttggcaaca agctgcagaa taacactctt cgaaaatctc
     3061 ttcaaaccac gtcagctcta tctgcaacct caaaacagtg ttttgagcaa cttgcggcta
     3121 gcttcctagt ttgctctttg attttcattg agtataaaca gccactctcc agttcaattg
     3181 gagagttttt tgatgagagt agatatttat agacctcaat ttgacaccat acattttcta
     3241 tgataaaatg taacaaattt atttacaaca aatcgaaatc taaatgaaat ggaattttct
     3301 atgaaatcag tcaaaggact actctttatc atagctagtt ttatcttgac tcttttgact
     3361 tggatgaaca cttctcccca attcatgatt ccaggactag ctttaacaag cctatctctg
     3421 acttttatcc tagccactcg tctcccacta ctagaaagct ggtttcacag tttggagaag
     3481 gtctacaccg tccacaaatt cacagccttt ctctcaatca tcctactaat ctttcataac
     3541 tttagtatgg gcggtttgtg gggctctcgc ttagctgctc agtttggcaa tcttgccatc
     3601 tatatctttg ccagcatcat ccttgtcgcc tatttaggca aatacatcca atacgaagct
     3661 tggcgatgga ttcaccgcct ggtttaccta gcctatattt taggactctt tcacatctac
     3721 atgataatgg gcaatcgtct ccttacattt aatcttctaa gttttcttgt tggtagctat
     3781 gcccttttag gcttactagc tggtttttat atcatttttc tatatcaaaa gatttccttc
     3841 ccctatctag ggaaaattac ccatctcaaa cgcttaaatc acgatactag agaaattcaa
     3901 atccatctta gcagaccttt caactatcaa tcaggacaat ttgcctttct aaagattttc
     3961 caagaaggct ttgaaagtgc tccgcatccc ttttctatct caggaggtca tggtcaaact
     4021 ctttacttta ctgttaaaac ttcaggcgac cataccaaga atatctatga taatcttcaa
     4081 gccggcagca aagtaaccct agacagagct tacggacaca tgatcataga agaaggacga
     4141 gaaaatcagg tttggattgc tggaggtatt gggatcaccc ccttcatctc ttacatccgt
     4201 gaacatccta ttttagataa acaggttcac ttctactata gcttccgtgg agatgaaaat
     4261 gcagtctacc tagatttact ccgtaactat gctcagaaaa atcctaattt tgaactccat
     4321 ctaatcgaca gtacgaaaga cggctatctt aattttgaac aaaaagaagt gcccgaacat
     4381 gcaaccgtct atatgtgtgg tcctatttct atgatgaagg cacttgccaa acagattaag
     4441 aaacaaaatc caaaaacaga gcttatttac gaaggattca agttcaaata agtaatcata
     4501 ctctcctggg atttcccagg agagtttttt atagccaaat gaaataagac aggaacgatg
     4561 gaattttggc attcaaatca attgataaga ctattttata gtggagtgaa ataggatacg
     4621 aacaaattga ttaggaaaat caaatgaatt tatagaaatc ttttagcagt tatgttatcc
     4681 tattctagtt tcaaaacgct atagaagcag cattgtgcta gtctagattc agtttactat
     4741 actaaaacga gtagcttgaa atcaaaaaac ccaccctcac aggcaggttt tatctgtatt
     4801 attcagctag attatgcttt accttctgaa ccgaatacgt cgatacgttc ttcaaccgat
     4861 gcttggatag cttttacacc gtcagccaag aatttacgtg ggtcgaagag ttttttcttg
     4921 tcgtattctg cttcgtttgc ttcgtagtca cgagcaaatt tacgagttgc gttagcgaat
     4981 gcgatttggc attctgtgtt aacgttaact ttggcaacac caagtttgat agctgcttgg
     5041 atttgctcat caggaatacc tgatccaccg tgcaatacga ttgggaatcc tggaagagct
     5101 tctgtcaatt tttgcaagtg gtcaaggtca agaccttccc agtttactgg gtaaggaccg
     5161 tggatgttac cgataccagc tgccaagaag tcgataccag tttcaaccat tgctttagcg
     5221 tcttcgattg gagccaattc acctttaccg atgattccat cttcttcacc accgatagta
     5281 ccaacttcag cttctactga gataccttta gcgtgtgctt tttcaacaac ttctttagcc
     5341 aatttaaggt tttcttcaac tggaaggtgt gaaccgtcaa acatgattga agtataacca
     5401 acttcgatac actcaagtgc atcttcgtag tgaccgtggt caaggtggat agctactggt
     5461 acagtgatac ccattgattc aacaaggtta gcgatcaagt tgcgagcaac tttgtaacca
     5521 cccatgtatt tagcagcacc cattgaagtt tggatcaaaa ctggagcttt tttagcttct
     5581 gctgcgcgca agatagcttg agtccactca aggttgtttg tgttaaatcc accaactgca
     5641 taaccgttgt cacgggctgc ttggacaaat ttttctgctg aaacgattgc cattttatca
     5701 ggcctcctgt atatttttat gggtcatccc atttacattg ttcattttat cactttttgc
     5761 caaaaaaatc tagtttttcc cgcagtttcg attgattttc ttctaactcc atctatgtaa
     5821 accctttctc tccctagtct tggacgactt ttggaaaatc tataaagaag gttaaactat
     5881 tctcctccat ctcgaaacga taagctaatt tttcatgttc taatagactc ttaaccacaa
     5941 agagccccat accagacccc ttgaccttgc gactggcatt gtcagaaaaa gactgggcta
     6001 gtttttcttg ttcctctgag ctacagctat tttcgataaa aagttctcct tctctttctc
     6061 caattcgaac taagccacct ggaacagagt gcttaatggc attgctgatg agattagaaa
     6121 gaatcaactt cataactgat gggtttagat aagcctgctg atgggtcaaa ctattgtcta
     6181 tctggagctc tctttccttg gctagcaagg cataatcttt gaccagattt tgcgtcatct
     6241 ggaggaggtc aattgtttcc ctatcatctc gcaattcctg cacagaagag agggaaagta
     6301 tctgcagaac atggtgattg agttcatcca caatccccaa ggcaactccc agatactggt
     6361 ctctatcctt ataacgaccg atattctctc tcatattttc gattaggatt ttcaaactag
     6421 ccagcggtgt tttcaattca tgagaagctc ctcgtaggaa ttcgaccttc atcttctcca
     6481 gctggagaat ggcttcattc ttttcatgca agtccgcaat aacagtcaag agatgctggt
     6541 agaggctatt gatttgttcc ttgagattac ctatctcatc cttagaatcc acgcgcaatc
     6601 gcacttggga atccaggtcc atcatccgac gggtcacccg cttgatttcc aaaatcggtg
     6661 caacaatagt ccgagcgtag atgtaggcca ccaaaaggga aatcagaaag gaggccagca
     6721 aggtataggg aagaaactgg agactgattt gctccgcttc cttttgtaaa tccatggaag
     6781 ctagaaactg gagaatcata gtaccaccgt cttgcgtttt cacctcgcgc tcctcaataa
     6841 agagagaggt tgtctggcgg tctgtgtcca gaggaagact gtccttgact tctaacttgt
     6901 cctcggtcat ctcacctttg acggtcccct tgatatcact agtctgggaa tacaagtcta
     6961 acacttgctc gatactctgc ctatctttcc cttctaggga ctgggcaatg gctgttgcct
     7021 tttgaccaat ggtttcctga cgatgactca gataagtcga aggaaaaaga aaataaatag
     7081 ctaaatgaag gcagataacc agaacactaa atatcgagaa ggtatagata aatatctttg
     7141 caaataaacc tgttcgtttc attttcgctc caatttataa ccaacattgc gcacagtgag
     7201 gatacaatcc aagtctagct ttttccgcaa ttccttgata taaacatcaa taacacggtc
     7261 aaagggaacc tcatctgtcg ctttccagac ggcatcgata atctgagatc gagtcaaggc
     7321 ccggccttca tttttcacta gatagtccag aatttccaac tctttggcat tgataggcac
     7381 ttcttgacct gcgaggcttg cactgtagct ttcaaagtcc accttggtat ccttgtaaga
     7441 aaagattcgt cctgtatcgt agtagcgctt gaaaatcgcg tccaccctca cttttaaaag
     7501 ggagagggag aaaggttttt ccagatagcc atctgccaaa gaggcaaagg cactcatctt
     7561 gtattcctca tcttgaaaag ctgtcaacat caagacagga acctgactgg ttttacgaat
     7621 ctcagctagg acttctaagc cgttgagctt gggcatctgg atatccagta aaaccagggc
     7681 cacctcatag ctagaaaatt gctccagagc ttcctgaccg tccgctgcct caatagtttc
     7741 atagccacaa tccgtcaaat aatcactgac cccctcacgg atcatctctt catcttctac
     7801 aattaaaatt ttcatacttt aactgctctc tattttttat ttttcttaga ataaatacct
     7861 accctatttt ctattatagt ctcttgctgg ccttttgtct gcaagcaact gaccactaga
     7921 taaaacgttg tgaaattcct ttctcataaa ttccataact ttagtatatt atatttaagc
     7981 actaaagtac aaagaaagca actgaaagca atgattttca ccactgcttt cggatttatt
     8041 ttgaattgtt aaatagccat tcctatccac tattcttgaa tagaaacaca agatgcaatc
     8101 tttattctag actcattttt tcaaatttat tcaccatcca gcaagagctc ttttggttgt
     8161 tttctaagga gattgcttga agcaagcgcc ataacgagaa ccactagaac caaggcaagg
     8221 acaaaaatga tgataaagtc tgatgtctga atggaaatgt ctaggctcga caaggtcttg
     8281 ctaaagccat ctacttctgc accaccacca aggttagagg cttgagccgc cttactagcc
     8341 tgtttggcaa cacctgaagt cacattggca aggacagtgt ttccaattgc acgggcagtg
     8401 taattagcta ggaagtaagc agaaactaga gcagggatag caatcaagat agattcggtg
     8461 atgaattgac ccaagatact tgcctgcttg aggccgatag agaggagaat tcccacttcc
     8521 ttgcgacggg cgttgatcca aaggctgagc aagagggcaa ggaggagaac tgagaagctc
     8581 aagctacccc agaagaggag gttggccatc ttgtacatac cagagataga ttgctcaaga
     8641 gctgggtagt tagaggagct cttgacgagt gtgtagctct tccagttgat accactgatg
     8701 ccattcaact ctttcataac atcatccaag ttcttgtctg ctgttacaaa gaaggttgcg
     8761 tccccataaa tggctgtgtc ttctgtgtat ccataaagtt ttgcagcagt gtgaatgtct
     8821 gtaatagctg tgttttcgta aagttcttgt gagtaggtta ctgctgactt attatgacca
     8881 tcaaagagtc ccttgattgt cacttcaact gtttccttgg ctcctttttc attatctgca
     8941 tcgtagatat tagagtccag tttaaccttg tcccctactt tccagccgtg tttggctgcc
     9001 aagtccttgt gcaagaggat tttatccttg tcgtcgttgg ttaagtgctc tccttcgact
     9061 agtttataag aaccagagac aaacttgtct tctttagagg agtcattgac acctgtaatc
     9121 atcaagctac ttccaaaacg cttggcacga tcagcagtga gattcttctt ggtttctggc
     9181 gtttcaatca ggtcatatcc agtcaaatct ccgatagcgt tgatacgttt gacataagac
     9241 tcaatggcct tgttttcggt gatttttttg atgtcttcac ccttgatatt cccagcacca
     9301 cgaggcgttc cttggttgac gcgacgattg atttgcatgg agaagctatt ggtgatattt
     9361 ttaaaggtct cctgagaagc cttggcagta gctcccttga ttgacaagcc gaccaaactc
     9421 aagctcgcca tgaggagaat aatcaggaag atgacaatcg atttgaaaaa cttccttgta
     9481 acataggcaa atgcgttgtg taacatagat tccctttcta gattttgttt taatcattct
     9541 attaaaataa gctcaaatta tttactagta ttgcgcgttt cagtcagttt cttatccttt
     9601 aattcaagtg taatatctga cgcttgtgcc acttctttac tgtgagttac gacaatcaca
     9661 catttacctg ttttctgggc aagtgatttg agtagttcga caatatctcc agcagtttta
     9721 ggatccagat ttcctgttgg ctcatcagct agaataactg gagcttctga gaccaaactg
     9781 cgagcaatgg caacacgttg ctgttgacca cctgataact ggagaacatt ccgcttgatc
     9841 tggctttcat ccaaaccaag ctcaagaagt gtattcttgc ttgccttttt gttgaccaat
     9901 cggatatttt ccagcggaga aagataatct atcaagttat aattttgaaa gaccagggaa
     9961 atatggtgca tgcgatggta agaatagccc ttcttacgaa tatcctctcc ttgaaaaagg
    10021 atagaacctt caacaggact atctagacca gcaagtaggg acaagagtgt ggattttcct
    10081 gctcctgact ccccaataat actgtaaaat tttccgggtt caaaattata attgatctga
    10141 tataggactg cttcagcagt attcttataa cggtaggtaa catcttgtaa ttgtaataaa
    10201 gtcatgattt ctccttctta actaatagat gataaaattt ctttcggtga ttttctaaat
    10261 aagaatagga aacaaagggc tacagataag caactaagca gaactagaaa aacataggat
    10321 tctgcaaaag ataagatgct agttgataaa ctgcttgctt tggctagtgt atcttgtaag
    10381 cttgcctgat ctccacttgc tagtagagtt tggagtaggt aagttgtgat tgcgtttcct
    10441 gcaacaaatg ctggaagcaa agctccaaga gataccaaaa ctacctctaa acagaattgt
    10501 aggaagatcg agctcttgcc ttttccaagt gcaagtaaaa tccccacttc atagacccgt
    10561 tctctcaacc agagagacaa aaccagaatt aaggctccag ctcctgctat caacatccca
    10621 taaaggaaga tggtcaggaa ggtttggaaa gttgcaactg agtctttgat ttgttcaaaa
    10681 gccttgtttt ccttttcgac ttggtagcct tgattttcca aggccaagtt ttctacctgc
    10741 ttcatgagtc cgtccatttc cttaggattt tctacataga agcgtgctgc actgacttga
    10801 gcttcactat tgcccaaaag ggtttggcta ctttcatagt ctgtaaagac ttgattttca
    10861 ctgaagtcag aagacaagcc tgtgaatttc tcttgttttt taccagaaaa gatgccgata
    10921 atctcaaact ctactgtttg tccttttcca gattcagact gaccagcatc caagccaatc
    10981 ttgtcatgaa gcgaaagacc gttcttctta gccaattctt cgtggataag gattttcttg
    11041 gaatcccctt tttgaaggtg tcgcccttct tttagattga aagccgaact ggtaaaggtt
    11101 acatccttgg atgaatcctc aagagccgtt aagctaacca agttattgtc tgcagctgat
    11161 aaatcatcac gctccacgct ctgctcgcca gtcactgctt ccttgtcttt tagttttgcg
    11221 accgtctcaa gttcaggaga gacattttcc agccccttaa tcttgcttac agatgctagg
    11281 tctgacaact tgaatgtctg accattctct atcttcttaa tagaaaaaga tgtattgagt
    11341 gatttataaa gattgctttc tactgttttg ttggacttca tcagagtcaa acaggctgaa
    11401 attccggcca ataagaccaa taaaatcaga aataaaataa aacttctcag tcgctttctg
    11461 ctgacataag cccaagatct ttggattgga ttcatttgtc acctccatat ttgtaagact
    11521 attataaaac ccaaatatga aatatttatg aaatacgaaa aaaaaatatc gagtagggga
    11581 taatctctag cccctctcac accacc
//
LOCUS       DAVKNL010000057        11551 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVKNL010000057 DAVKNL010000000
VERSION     DAVKNL010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11551
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.057"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            <1..416
                     /locus_tag="VW411_001668"
     CDS             <1..416
                     /locus_tag="VW411_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9794364.1"
                     /translation="LILLYLTYAIPQTLFLYVGYIKISIPESLDEAAEIDGANQFTTY
                     FRIIFPMMKPMHATTMIINALWFWNDFMLPLLVLNRDSKMWTLPLFQYNYAGQYFNDY
                     GPSFASYVVGIISITIVYLFFQRHIISGMSNGAVK"
     gene            417..497
                     /locus_tag="VW411_001669"
                     /pseudo
     CDS             417..497
                     /locus_tag="VW411_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            774..2216
                     /gene="gtfA"
                     /locus_tag="VW411_001670"
     CDS             774..2216
                     /gene="gtfA"
                     /locus_tag="VW411_001670"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEU9794365.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGEKYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKALFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQIYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVVEIDLQNQTYRVIENGVEVSF"
     gene            2354..3547
                     /locus_tag="VW411_001671"
     CDS             2354..3547
                     /locus_tag="VW411_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU9794366.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMNSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            4232..6211
                     /locus_tag="VW411_001672"
     CDS             4232..6211
                     /locus_tag="VW411_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9794367.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAARETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            6278..7774
                     /locus_tag="VW411_001673"
     CDS             6278..7774
                     /locus_tag="VW411_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9794368.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPAAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            7774..8700
                     /locus_tag="VW411_001674"
     CDS             7774..8700
                     /locus_tag="VW411_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9794369.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            8709..9776
                     /locus_tag="VW411_001675"
     CDS             8709..9776
                     /locus_tag="VW411_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9794370.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            9787..10713
                     /locus_tag="VW411_001676"
     CDS             9787..10713
                     /locus_tag="VW411_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9794371.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            complement(10788..11551)
                     /locus_tag="VW411_001677"
                     /pseudo
     CDS             complement(10788..11551)
                     /locus_tag="VW411_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249392.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3394 a         2060 c         2437 g         3660 t
ORIGIN      
        1 gtttgatctt gctctacttg acctatgcga ttccacagac cctctttctc tatgttggct
       61 atatcaaaat ctcgattcca gaaagtctgg atgaagcagc agagatcgat ggggctaatc
      121 aatttacaac ctatttccgc atcatcttcc caatgatgaa accgatgcat gcgacaacca
      181 tgatcatcaa tgccctttgg ttctggaatg acttcatgtt gccactcctt gtcttgaacc
      241 gggattccaa aatgtggact ctgcctttgt tccaatacaa ctacgcaggc caatatttca
      301 acgactacgg accaagcttt gcctcttacg tggtcggcat tatcagtatc accattgtct
      361 atctcttctt ccaacgccat atcatttcag gaatgagcaa cggggcagtg aagtaatact
      421 caatgaaaat caaagagcaa actaggaagc tagccgcagg ttgctcaaaa cactgttttg
      481 aggttgtgga tgaaactgac gaagtcagta accataccta cggcaaggtg aagctgacgt
      541 ggtttgaaga gattttcgaa gagtataaga agagctcatg gaatctatag tctttgacac
      601 agaggaagag tttagcttgt tgataattct atgcagaaaa caagaaagta gtactttgtt
      661 tatcacagtc tatattagta cgagtttgat gataaataga gatttaaaat gaattttatt
      721 tatgaagcca gatcatatga gacttggctt caattagaaa aaggagagta atgatgccaa
      781 ttcaaaataa aaccatgttg attacctatt ctgatagcct tggaaataat cttaaagact
      841 tatatgataa tttggaagag cattttggag atgctattgg aggagttcac cttttaccat
      901 ttttcccatc aacaggtgat cgtggatttg cgccagttga ctacgacgaa gtggactcag
      961 cttttggtga ttgggaggat gtgaagcgtt taggtgagaa atattatctt atgtttgatt
     1021 ttatgattaa tcatatttct cgtcaatcca agtattataa ggactatcaa gaaaaacatg
     1081 aagccagtga atttaaagct ctctttttaa actgggataa gttttggcca gaaaaccgtc
     1141 cgacacagtc tgatgtagat ttaatttaca agcgtaagga tcgtgcacca aagcaagaga
     1201 ttgtgtttga agatggttca gtggaacatt tgtggaatac ctttggtgag gagcagattg
     1261 atcttgatgt gaccaaagaa gtaactatgg aatttatccg taagaccatt cagcacttgg
     1321 caagtaatgg gtgtgatttg attcgtctag atgcctttgc ttatgcagtg aagaaattgg
     1381 atactaatga tttctttgtg gaaccagata tttgggattt attggacaaa gttcgagata
     1441 tcgctgctga gtatgggaca gagcttttac ctgagattca tgaacactat tcgattcagt
     1501 ttaaaatagc agaccatgat tactatgttt atgattttgc tcttccaatg gtgacacttt
     1561 atactcttta cagttccaga acagagcgtt tggctaagtg gttaaagatg agcccgatga
     1621 agcaatttac gacgctagat acccatgatg ggattggagt agtagatgtc aaggatatcc
     1681 tgaccgatga ggagattgac tatgcttcaa atgaactcta taaggttgga gccaatgtca
     1741 aacgtaagta ctctagtgcc gagtataaca acttagatat ctaccaaatc aattcaacct
     1801 actattcagc gcttggagat gatgatgtca agtattttct cgctcgtcta attcaagctt
     1861 ttgccccagg tattcctcag atttactatg tgggtctatt agcaggcaag aatgacttga
     1921 aattattaga agaaactaaa gaaggtcgaa atattaatcg tcattactat agcaacgagg
     1981 aaatagcaaa agaagtgcaa cgacctgttg tgaaggccct tctcaatcta ttttctttcc
     2041 gtaaccgttc agaagccttt gatctagaag ggactactga gatagagaca ccaacagctc
     2101 acagcattgt aatcaaacgt caaaataaag ataagtccgt aacagcagta gtagaaattg
     2161 atttgcaaaa tcagacttat cgggtaattg agaatggagt tgaagtatca ttttgaagcc
     2221 ttgatatggt ggataaaata gggtttttat tttggaaaac gtttcctttg ttttcaaatt
     2281 gctaaaaaag tggtacaata taggatagct tactattatc tgaatcagct gatttggaga
     2341 gaaaggattc attttgaaat caataggctt tattgaaaag ctgaaggggt tgtctagtaa
     2401 agagctgatt ttattgggaa ttatcctgag tatcttttta cccttttatc tttttgtagt
     2461 tgtactctgt ttatatatta tcagtttgat ttttacagga gacatgaaaa gtattcttca
     2521 gaaaatgggg gagcatccga tgctgcttct ttttcttagc tatagtactg ttatatccat
     2581 tcttgcacaa aattggatgg gtcttgtggc ttcagtagga atgtttctat ttactatttt
     2641 ctttttgcac tatcagtcga ttttatccca taaattcttt cgattgattt tgcagtttgt
     2701 cttgtttggt agtgtcttgt cagctgcttt tgccagttta gaacatttcc aaattgtgaa
     2761 gaaatttaac tatgcttttc tttcacccaa tatgcaggtg tggcatcaga accgggcaga
     2821 agtgaccttc tttaatccta attattatgg aattatttgt tgtttctgta ttatgattgc
     2881 tttctatctg tttacaacga ccaagttgaa ttggttgaaa gtattctgtg tgattgcagg
     2941 ctttgttaat ctctttggtt tgaactttac tcaaaatcga actgcctttc ctgctattat
     3001 cgctggagca attatctatc tctttacgac tattaaaaac tggaaggcct tttggcttag
     3061 tattggggtc ttcgcgattg gtttgagttt cctcttttct agtgatttgg gagttcgaat
     3121 gggtacttta gactcttcta tggaagaacg catttctatc tgggatgctg ggatggcctt
     3181 gtttaagcaa aatccttttt ggggtgaagg gccattgacc tatatgaact cttatcctcg
     3241 gatacatgct ccttatcatg aacatgccca cagtctttat attgatacga ttctgagtta
     3301 cggaattgtg gggactattt tattagtttt gtcttctgtt gctcctgttc gcttgatgat
     3361 ggatatgagt caggagtcgg ggaaacgtcc gattatcggc ctttatctat ctttccttac
     3421 agtggttgct gtgcacggaa tttttgactt ggctctcttc tggattcagt caggctttat
     3481 tttcttgcta gttatgtgca gtattccatt ggagcatcga atgttggtat cggacatgac
     3541 ggattaagtt ttataagttt gaaatcttct accttgggta ggagattttt tatacgatgg
     3601 gcatgtcgtg catctgaggt gtaagtcctc tgtagggctc cactaccggt gaatacaata
     3661 gcgctcccca agcctgacta tcgtgaggta gcggattaaa atggtctggg gatagatcgt
     3721 tttaagtctg acgcaggaaa taagaattgt cagagaaagc aatagtgaaa tcgtaggcta
     3781 tgaatagaaa cgtgatagcg aggagcatat agtggataag gtagcgagaa attttaaagt
     3841 ataaaagata gtcttaatct atatatgtaa atcacaaaag taattgaatt cgggataagg
     3901 ttggtgtgaa aagacatagt aaactgaatc ttgaatacta gaatgatact tctacaatat
     3961 tgttataatt taatttgaat tccaaaatca cattgttccg tccgtatttc attttactgt
     4021 atgtcttcct aaactccaaa gtttctgcgt caaatttcct attttcactg tagtctttga
     4081 cacttcgaac gacaatttga atcatatatt caaaaaaaat gaaaaatttg tgccaacaag
     4141 cctaaatatt ctgaaaattt gtttgatttt taatggataa tgtgatataa tggttgtcaa
     4201 tctataattc acataaacag aggagtagaa aatgaaaaaa aatcgtgtat ttgctacagc
     4261 aggtcttgtt ttattagcag caggtgtact tgcagcatgc agttcttcaa aatcatctga
     4321 ttcatcagcc cctaaagctt atggctatgt ttatacagca gacccagaaa ccttggacta
     4381 cctgatttca agtaaaaata gtacaacagt agtgacttca aatgggattg atggtttatt
     4441 cactaacgat aattacggta atcttgctcc tgcagttgca gaggattggg aagtctctaa
     4501 ggatggtttg acctacactt ataagattcg taaaggggtt aaatggttta cctctgatgg
     4561 agaagaatat gcagaggtga cggctaaaga tttcgtgaac ggtttaaaac acgcagcaga
     4621 taaaaaatca gaagctatgt atttagctga aaattcggtt aaaggcttgg cagattatct
     4681 atcaggaact tcaacagatt tttcaacagt tggtgtcaag gcggttgatg attatacgtt
     4741 acaatacact ttgaaccagc ctgaaccgtt ctggaactct aagttgacct attctatttt
     4801 ctggcctctg aatgaagaat tcgaaacatc aaaaggaagc gattttgcta aaccaacaga
     4861 tccgacatcc ttgctttata atggtccatt cttgttgaaa gggttgactg caaaatcttc
     4921 tgtagagttt gtaaaaaatg agcaatattg ggataaagaa aatgtccacc tagatactat
     4981 caatctagct tactatgatg gatcagatca ggagtcgcta gagcgtaact tcactagtgg
     5041 agcttatagt tatgcccgtc tttaccctac cagctccaac tattctaagg ttgcagaaga
     5101 atacaaggac aatatctatt acacacaatc aggctctggg attgctggtc tgggtgtgaa
     5161 tattgatcgc caaagttaca actatacttc taaaactaca gattcagaga aagtagctac
     5221 taagaaggca ttgcttaaca aagatttccg tcaagccttg aattttgctc ttgatcgctc
     5281 agcttactca gctcaaatca atggtaaaga tggagcagct ttagcagttc gtaatttatt
     5341 tgtaaaacca gactttgttt cagctggtga gaagaccttt ggtgatttag tcgctgctca
     5401 acttcctgct tatggtgatg agtggaaagg tgtgaattta gctgatgggc aggatggttt
     5461 attcaatgct gacaaggcca aggcagagtt tgcgaaagct aagaaagctt tagaagcaga
     5521 cggcgttcag tttcctattc atctggacgt tccagtagac caagcatcaa aaaactacat
     5581 atctcgtatt cagtccttta aacaatctgt agaaacagtt cttggtgttg aaaatgtcgt
     5641 tgttgatatt caacaaatga caagtgatga attccttaat attacttact atgctgccaa
     5701 tgcttcatct gaggattggg atgtatcagg aggagtttca tgggggccag actatcaaga
     5761 cccatctact tacctggata ttttaaaaac aactagcagt gaaactacaa aaacatattt
     5821 aggatttgat aatccaaata gcccttcagt agttcaagtt ggtttgaaag aatacgataa
     5881 attagttgat gaagctgcca gagagacaag cgacttgaat gtccgttatg aaaaatatgc
     5941 agcggctcaa gcatggttga cagatagttc actctttatt cctgctatgg cttcttctgg
     6001 tgcagcacca gtgctttcac gaattgttcc atttactgga gcttctgcgc aaacaggctc
     6061 taaggggtca gatgtttact tcaaatattt gaaattacaa gataaagtgg tgactaagga
     6121 agagtatgaa aaagctcgtg aaaaatggtt gaaagaaaaa gctgaatcaa atgagaaagc
     6181 tcaaaaagaa ttggcaagtc atgtgaagta aataatttct aatagaactt tctctccatg
     6241 aggagaaggt tcttttggga tttttaaagg aaataatatg aaaaaatata tttttatgcg
     6301 tgttttgcgg tcattggttt cgattttctt agtaacgact ttgacctaca cgattatcta
     6361 taccttggtt cctcgaaaat tgattttcaa gcaggatcct aactataata aaattgcgac
     6421 aacggctgat aaacgtgata actatgaaaa tactgtgttt gagcgtatgg gctacattga
     6481 gtattacgat actaaagagt tgcaagaaaa ggcaagtagc atggattctt ctgtaacagt
     6541 agaagcaaat gcgaccaata aagctattta tgaaaagtac atcaatcaat taggtcatgg
     6601 ttggactttg ggagaattta ctgaaagtgg tcaattctat gctactcgtg aaattccaat
     6661 ttttgaacgt gtttttcact tctatgctaa cttgattgac attgaccata caaataaaat
     6721 ccaagaccct gaaaatccag acttgaaacg ctaccttcgt tttgaaaatg atccagctat
     6781 cggatggtca ttggtcggtt caggaactaa acataaatat ctcttgtact ttaacagtca
     6841 gttcccattt gttcatcaaa actttgtgaa cttgaattta ggtgactctt acccaaccta
     6901 tgctaataca ccagttcttc aggttattac tcaaggtcaa ggacaaacca aaactgccca
     6961 agttcagttc ccaacaggta agaaaacgtc ttctgtaaat atttactcaa gaacctacaa
     7021 gtcacctagt caggctgact ctcgtgaagt agctagctat gggaaagatg atccttatac
     7081 agcgactgaa agtaattacc aatatccatc tatgattgtc agctctgcta ttactggttt
     7141 gattggtttg gttcttgcct atgctcttgc cgtgccactt ggttcagcca tggctcgttt
     7201 caagaacact tggattgata gcctctcaac aggggctttg accttcttgc ttgctcttcc
     7261 aacgattgcc ttggtttaca tcgttcgatt gattggatca tctattgccc ttccagattc
     7321 attccctatc ttgggagctg gagattggcg ttcttacgtt ttaccagcag tcatccttgg
     7381 tttgttgggt gctcctggta cagccatttg gattcgtcgt tacatgattg acttgcaatc
     7441 tcaagacttt gttcgtttcg ctcgtgcaaa aggtttgtct gaaaaagaaa tttcaaacaa
     7501 acacatcttt aaaaatgcca tggttccgct ggtttcagga attcctgctg ccattattgg
     7561 ggttatcggt ggtgcaaccc ttactgaaac agtcttcgcc ttcccaggta tgggtaaaat
     7621 gttgattgac tctgtaaaag catctaataa ctctatggtc gttggtcttg tcttcatctt
     7681 tacatgtatt tctatcttct cacgtctttt gggagatatt tggatgacta ttattgaccc
     7741 acgtattaaa ttgactgaga aaggaggcaa ataatgtcta caatcgataa agaaaaattt
     7801 cagtttgtaa aacgtgacga ttttgcctct gaaactattg atgcgccagc atattcttac
     7861 tggaaatcag tgtttaaaca atttatgaag aaaaaatcaa ctgtagtcat gttgggaatc
     7921 ttggtagcca tcattttgat aagtttcatc tacccaatgt tttctaagtt tgatttcaat
     7981 gatgtcagca aggtaaacga ctttagtgtt cgttatatca agccaaatgc ggagcattgg
     8041 ttcggtactg acagtaacgg taaatcgctc tttgacggtg tctggttcgg agctcgtaac
     8101 tccatcctca tttctgtgat tgcgacagtg attaacttgg ttatcggtgt ttttgtcggt
     8161 ggtatttggg gtatttcaaa atcagttgac cgtgtcatga tggaagttta caacgtcatc
     8221 tcaaacatcc cacctctttt gattgttatt gtcttgactt actcaatcgg agctggattc
     8281 tggaatctga tttttgccat gagcgtaaca acatggattg gtattgcctt catgatccgt
     8341 gtgcaaatct tgcgctatcg tgacttggaa tacaacttgg cgtcacgtac tttgggaaca
     8401 ccaaccttga agattgttgc caaaaatatc atgcctcaat tggtatctgt tattgtgaca
     8461 accatgactc aaatgcttcc aagctttatc tcatacgaag ccttcttgtc tttcttcggt
     8521 cttggattac cgattacagt gccaagtttg ggtcgtttga tttcggatta ttcacaaaac
     8581 gtaacaacca atgcttactt gttctggatt ccattgacaa cccttgtctt ggtatccttg
     8641 tcccttttcg tagttggtca aaacttagcg gatgctagtg atccacgtac acatagatag
     8701 gagtagaaat gacaaaagaa aaaaatgtaa ttttgactgc tcgcgatatt gtcgtggaat
     8761 ttgacgttcg tgacaaagta ttgacagcca ttcgcggcgt ttcccttgaa ctagtcgaag
     8821 gagaagtatt agccttggta ggtgagtcag gatcaggtaa atctgttttg acaaagacct
     8881 tcacaggtat gctcgaagaa aatggtcgta ttgcccaagg tagtattgac taccgtggtc
     8941 aggacttgac agctttatct tctcacaagg attgggaaca aattcgtggt gctaagattg
     9001 cgactatctt ccaggaccca atgactagtt tggaccccat taaaacaatt ggtagtcaga
     9061 ttacagaagt tattgtaaaa caccaaggaa aaacagctaa agaagcgaaa gaattggcca
     9121 ttgactacat gaataaggtt ggcattccag acgcagatag acgttttaat gaatacccat
     9181 tccaatattc tggaggaatg cgtcaacgta tcgttattgc tattgccctt gcctgccgac
     9241 ctgatgtctt gatctgtgat gagccaacaa ctgccttgga tgtaactatt caagctcaga
     9301 ttattgattt gctaaaatct ttacaaaacg agtatcattt cacaacaatc tttattaccc
     9361 acgaccttgg tgtggtggca agtattgcgg ataaggtagc ggttatgtat gcaggagaaa
     9421 tcgttgagta tggaacggtt gaggaagtct tctatgaccc tcgccatcca tatacatgga
     9481 gtctcttgtc tagcttgcct cagcttgctg atgataaagg ggatctttac tcaatcccag
     9541 gaacacctcc gtcactttat actgacctga aaggggatgc ttttgccttg cgttctgact
     9601 acgcaatgca gattgacttc gaacaaaaag ctcctcaatt ctcagtatca gagacacatt
     9661 gggctaaaac ttggcttctt catgaggatg ctccgaaagt agaaaaacca gctgtgattg
     9721 caaatctcca tgataagatc cgtgaaaaaa tgggatttgc ccatctggct gactaggagg
     9781 aaggaaatgt ctgaaaaatt agtagaaatc aaagatttag aaatttcctt cggtgaagga
     9841 agtaagaagt ttgtcgcggt taaaaatgct aacttcttta tcaacaaggg agaaactttc
     9901 tcgcttgtag gtgagtccgg tagtgggaaa acaactattg gtcgtgctat catcggtcta
     9961 aatgatacaa gtaatggaga tatcattttt gatggtcaaa agattaatgg taagaaatcg
    10021 cgtgaacaag ctgcggaatt gattcgtcga atccagatga ttttccaaga ccctgccgca
    10081 agtttgaatg aacgtgcgac tgttgattat attatttctg aaggtcttta caatcaccgt
    10141 ttatttaagg atgaagaaga acgtaaagag aaagttcaaa atattatccg tgaagtaggt
    10201 cttcttgctg agcacttgac tcgttaccct catgaattct caggcggtca acgtcaacgt
    10261 atcggtattg cccgtgcctt ggtcatgcaa ccagactttg ttattgcaga tgagccaatt
    10321 tcagccttgg acgtttctgt acgtgcccaa gtcttgaact tgctcaaaaa attccaaaaa
    10381 gagctcggcc tgacctatct cttcatcgcc catgacttgt cggttgttcg ctttatttca
    10441 gatcgtatcg cagttattta caagggtgtt attgtagagg ttgcagaaac agaagaattg
    10501 tttaacaatc caattcaccc atatactcaa gccttgcttt cagcggtacc aatcccagat
    10561 ccaatcttgg aacgtaagaa ggtcttgaag gtttacgatc caagtcaaca cgactatgag
    10621 actgataagc catctatggt agaaatccgt ccaggtcact atgtttgggc gaaccaagcc
    10681 gaattggcac gttatcaaaa aggactaaac tagtaatggt tttataattt ccatgtcaat
    10741 ttttatggaa attaggctct ttgtcaactg tagtgggttg aagtcagcta agctcgagaa
    10801 aggacaaatt tcgtcctttc ttttttgatg ttcaaagcga taaaaatccg ttttttgaag
    10861 ttttcaaagt ttcgaaaacc aaaggcattg cgcttgataa gtttgatgag attattggtc
    10921 gcttccagtt tggcattaga atagtgtagt tgaagggcgt tgataatctt ttctttatct
    10981 ttgagaaagg ttttaaagac agtctgaaaa agaggatgaa cctgcttcag attgtcctca
    11041 atgagtccga aaaatttctc agggtctttg ttctgaaagt gaaaaagtaa gagttgatag
    11101 atctgatagt ggtgtttcaa gtcttctgaa tagcttaaaa tcttgtcaag aatttcttta
    11161 tttgttaagt gcatgcgaaa agtagggcga taaaatcgct tatcactcag tttacggcta
    11221 tcctgttgaa tgagtttcca gtagcgcttg atagccttgt attcatggga ttttcgatga
    11281 aactgattca tgatttggac atgcacacga ctcatagcac ggctaagatg ttgtacaatg
    11341 tgaaagcgat caagaacgat tttagcattc gggagtgaaa cagcctggga gactgtttca
    11401 gcctgagcct agaaatttga aagcgaagct gtttagccaa gtcatagtaa gggctaaaca
    11461 tagccatagt aatgattttg acgcgacatc ggacggctct atcatattta agaaagtgat
    11521 ctcggatgac agcttgtgtt ctaccttcaa g
//
LOCUS       DAVKNL010000058        11503 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVKNL010000058 DAVKNL010000000
VERSION     DAVKNL010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11503
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.058"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(98..1729)
                     /locus_tag="VW411_001678"
     CDS             complement(98..1729)
                     /locus_tag="VW411_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEU9794372.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            2022..7001
                     /locus_tag="VW411_001679"
     CDS             2022..7001
                     /locus_tag="VW411_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEU9794373.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEGVAIASETASPASNEAATTE
                     TAEAASAAKPEEKASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEK
                     EVQPDVPKNTEKTLKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNE
                     KASKEAKVQALSNTNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDA
                     GHRNGVPVYGTLFFNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFI
                     NQETTGDLVKPLGEKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYN
                     YQFMQPEGDKVPADNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKT
                     KVKWNDILDENGKLRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGD
                     KDWYGIANLVADRTPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWR
                     WWQTSTGEKLRAEYDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVA
                     HKGGKGSKVYMAFSTTPDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFF
                     EHEGAVKDYQFNLGQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADF
                     YEVYEKDGDSWKLLTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTT
                     FDWGMTVKDTSLPKPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSA
                     QLSGSVDIRLTKPRTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEV
                     RGNKAHVTDITLDKPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMA
                     KAAARSLGNNKVQVGFADVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQ
                     SGLLYYRTQLPGKEISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLR
                     VQYEGGTEDELIRLTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEAS
                     PKTILGIEVSQEPKKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQK
                     TGRQTLTLHYQGHEVSFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASSEVKEA
                     FLKAIEAAEQVLKDHETSTQDQVNDRLNKLTEAHKALNGQEKFTEEKTELDRLTGEVQ
                     ELLAAKPNHPSGSALAPLLEKNKALVEKVDLSPEELTTAKQSLKDLVALLKEDKPAVF
                     SDSKTGVEVHFSNKEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVD
                     LSYASIVKIPIEKDKKVKKVFFLPEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESA
                     EKPQPAKPAPQNTVLPKPTYQPTSDQQKAPKLEVQEEKVAFHRQEHENTEMLVGEQRV
                     IIQGRDGLLRHVFEVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQ
                     NTAVKSEEASKQLPNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            7091..8287
                     /locus_tag="VW411_001680"
     CDS             7091..8287
                     /locus_tag="VW411_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU9794374.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADNVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            8423..8950
                     /locus_tag="VW411_001681"
     CDS             8423..8950
                     /locus_tag="VW411_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU9794375.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETILYVFVRVLETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            9027..9383
                     /gene="glnR"
                     /locus_tag="VW411_001682"
     CDS             9027..9383
                     /gene="glnR"
                     /locus_tag="VW411_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEU9794376.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            9420..10766
                     /gene="glnA"
                     /locus_tag="VW411_001683"
     CDS             9420..10766
                     /gene="glnA"
                     /locus_tag="VW411_001683"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEU9794377.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(10862..10981)
                     /locus_tag="VW411_001684"
     CDS             complement(10862..10981)
                     /locus_tag="VW411_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEU9794378.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            11239..11376
                     /locus_tag="VW411_001685"
     CDS             11239..11376
                     /locus_tag="VW411_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794379.1"
                     /translation="MNPDRAEEYFCRGCQGENPEDIEFYDEQLQAEKVEDLNIRLEVK
                     N"
BASE COUNT         3677 a         2122 c         2495 g         3209 t
ORIGIN      
        1 tatatatctg cagaatactc tttcagtttc attcatccta gtcttgctta ggaaaaagta
       61 aaaagccatc caattatgat ggatagcttc ttggttctta gatttgttct gcaaaaacct
      121 tttcagcaag gttcatagcg tggtctgata cacgagtgta gtgtgagatg atgtcgataa
      181 agttgacccc agcttgtgtt gaacattcgc ctttgttgag gcgtttgatg tgggtttttc
      241 tgagaacacg ttctatctta ttgattgctt catgacgttc tacaagactg cgtgcttttt
      301 caatatcatt gttttccaca ctatccagag catctttgat aaagtcacta gtttggcggt
      361 aaacttcctc taattctttc aaggcggcat cagaaaattc aacatttttc cgttgaagat
      421 agtcagtcag attgagtaga gcctccgtgt ggtctccaat ccgttccaaa tcacgggagg
      481 aatcaaggat attggtaagc acttcacttt ctttttggct gagagcttcg cttgaaaggg
      541 caatgagata acgtgttaat tgctcatcga tggtgttaat tgcttcttcg gttttatgcc
      601 ctttttcagc aactttttca tccaagtcaa tgatgtactt ataggaaagg tcaaaggctt
      661 tagcagcgta gtttcctaag tgcaagagct ctttcttagc atttcctaga gcgatagatg
      721 gggcctgttt gatgaaatgt tcatcaagat ataagggttc gtatttgaca acctcgtcct
      781 ctccaggaat aatcttggtt acaaagtaag ccagagctcc gataaatgga aattggacaa
      841 tggtgttggt aatattaaag gttccgtgag caaaggcgat ggtcatttcc ggtgctagat
      901 ttagcgtagc ttcaaaccaa tgaatcagga cagtaaaagg aactagaaaa ataacgcaga
      961 caactgttcc gataacgttg aaggcaacat gagctcctgc tacccgttta gctgcaatat
     1021 tagcccctaa agaggcaatg atggctgtaa tggttgtccc gatattgtca ccaaatagaa
     1081 ctggcaaagc tccctgtaga tcaattagat tgccggcgta gaggttttgt aaaatcccaa
     1141 tggtagccga agaagcttga attagcaagg tcaagccagt accgacaaag acacccaaaa
     1201 caggattctt acttagctca atcatatagt ccttaaagac ctgtaaatcc ttgagtggag
     1261 ccattgcgcc gctcatgaga ttgagggcaa aaaagatacc accgacacca aagaggatgc
     1321 gtccgatatt attgactgtc cgatttttcg taaaaaaaag acagacggca ccgataaaga
     1381 gcataggtag ggcatagtta cctaatttaa aaccgatgag aaaggatgtg acagttgtcc
     1441 caatattagc acccatgaca atcccgatag cctgacgtaa ggttaagaga ccggcactga
     1501 ccaggccgac tgtgataact gttacaccag aactagactg aattagagca gtcatcccaa
     1561 taccaaccag aactccaaag aaaggattac tagtatattt gtcaatgtaa aaacgaaggc
     1621 gatctccagc agcttgttgt aaaccgtctc ccatggtctt gatgctatat aagaatagcc
     1681 ccagaccacc taaaaagtga aataaaattt cctgccaatt aatggacatt tctttttcct
     1741 ccgaaaaata atcgcggaat atctcctatt ctattttaaa ggataaaagt aaatctaaca
     1801 agtgttaatg tcaagtttgt aaaagaaaat tataattgat aagtgtatta tttttaagag
     1861 tttttaacct tattttagat ttgtgcaaac agttattgtt ttttgtctaa aaatgtttta
     1921 ggataaaaat gtgattatca tggttctaga gtttgataaa aaactacaga atataaagcg
     1981 ctttcttgaa aacaacaaaa tcaatctttt aggaggagaa aatgaagaat ccattttttg
     2041 aaagacgttg tcgttacagt attcgtaagt tatcagtagg agcctgctcg ctgatgattg
     2101 gtgctgtttt atttgctggt ccagccttgg ctgaagaaac tgcagttcct gaaaatagcg
     2161 gagctaatac agagcttgtt tcaggagaga gtgagcattc gaccaatgaa gctgataagc
     2221 agaatgaagg ggaacatgct agagaaaaca agctagaaaa ggcagaagga gtagcgatag
     2281 catctgaaac tgcttcgcca gcaagcaatg aagctgcaac tactgaaact gcagaagcag
     2341 ctagcgcagc taaaccagag gaaaaagcaa gtgaggtggt tgcagaaaca ccatctgcag
     2401 aagcaaaacc taagtctgac aaggaaacag aagcaaagcc cgaagcaact aaccaagggg
     2461 atgagtctaa accagcagca gaagctaata agactgaaaa agaagtccag ccagatgtcc
     2521 ctaaaaatac agaaaaaaca ttaaaaccaa aggaaatcaa atttaattct tgggaagaat
     2581 tgttaaaatg ggaaccaggt gctcgtgaag atgatgctat taaccgcgga tctgttgtcc
     2641 tcgcttcacg tcggacaggt catttagtca atgaaaaagc tagcaaggaa gcaaaagttc
     2701 aagccttatc aaacaccaat tctaaagcaa aagaccatgc ttctgttggt ggagaagagt
     2761 tcaaggccta tgcttttgac tattggcaat atctagattc aatggtcttc tgggaaggtc
     2821 tcgtaccaac tcctgacgtt attgatgcag gtcaccgtaa cggggttcct gtatacggta
     2881 cactcttctt caactggtct aatagtattg cagatcaaga aagatttgct gaagctttga
     2941 agcaagacgc agatggtagc ttcccaattg cccgtaaatt ggtagacatg gccaagtatt
     3001 atggctatga tggctatttc atcaaccaag aaacaactgg agatttggtt aaacctcttg
     3061 gagaaaagat gcgccagttt atgctctata gcaaggaata tgctgctaag gtaaaccatc
     3121 caatcaagta ttcttggtac gatgccatga cctataacta tggacgttat catcaagatg
     3181 gtttgggaga atacaactac caattcatgc aaccagaagg agataaggtt ccggcagata
     3241 acttctttgc taactttaac tgggataagg ctaaaaatga ttacactatt gcaactgcca
     3301 actggattgg tcgtaatcct tatgatgtat ttgcaggttt ggaattgcaa cagggtggtt
     3361 cctacaagac aaaggttaag tggaatgaca ttttagacga aaatgggaaa ttgcgccttt
     3421 ctcttggttt atttgcccca gataccatta caagtttagg aaaaactggt gaagattatc
     3481 ataaaaatga agatatcttc tttacaggtt atcaaggaga ccctactggc caaaaaccag
     3541 gtgacaaaga ttggtatggt attgctaacc tagttgcgga ccgtacgcca gcggtaggta
     3601 atacttttac tacttctttt aatacaggtc atggtaaaaa atggttcgta gatggtaagg
     3661 tttctaagga ttctgagtgg aattatcgtt cagtatcagg tgttcttcca acatggcgct
     3721 ggtggcagac ttcaacaggg gaaaaacttc gtgcagaata tgattttaca gatgcctata
     3781 atggcggaaa ttcccttaaa ttctctggtg atgtagccgg taagacagat caggatgtga
     3841 gactttattc tactaagtta gaagtaactg agaagaccaa acttcgtgtt gcccacaagg
     3901 gaggaaaagg ttctaaagtt tatatggcat tctctacaac tccagactac aaattcgatg
     3961 atgcagatgc atggaaagag ctaacccttt ctgacaactg gacaaatgaa gaatttgatc
     4021 ttagctcact agcgggtaaa accatctatg cagtcaaact atttttcgag catgaaggtg
     4081 ctgtaaaaga ttatcagttt aacctaggac aattaactat ctcggacaat caccaagagc
     4141 cacaatcgcc gacaagcttt tctgtagtga aacaatctct taaaaatgcc caagaagcgg
     4201 aagcagttgt gcaatttaaa ggcaacaagg atgcagattt ctatgaagtt tatgaaaaag
     4261 atggagacag ctggaaatta ctaactggct catcttctac aactatttat ctaccaaaag
     4321 ttagccgctc agcaagtgct cagggtacaa ctcaagaact gaaggttgta gcagtcggta
     4381 aaaatggagt tcgttcagaa gctgcaacca caacctttga ttggggtatg actgtaaaag
     4441 ataccagcct accaaaacca ctagctgaaa atatcgttcc aggtgcaaca gttattgata
     4501 gtactttccc taagactgaa ggtggagaag gtattgaagg tatgttgaac ggtaccatta
     4561 ctagcttgtc agataaatgg tcttcagctc agttgagtgg tagtgtggat attcgtttga
     4621 ccaagccacg taccgttgtt agatgggtca tggatcatgc aggagctggt ggtgagtctg
     4681 ttaacgatgg cttgatgaac actaaagact ttgaccttta ttataaagat gcagatggtg
     4741 agtggaagct agctaaggaa gtccgtggta acaaagcaca cgtgacagat atcactcttg
     4801 ataaaccaat cactgctcaa gactggcgct tgaatgttgt cacttctgac aatggaactc
     4861 catggaaggc tattcgtatc tataactgga aaatgtatga aaagcttgat actgagagtg
     4921 tcaatattcc gatggccaag gctgcagccc gttctctagg caataacaag gtacaagttg
     4981 gctttgcaga tgtaccggct ggagcaacta ttaccgttta tgataatcca aattctcaaa
     5041 ctccgctcgc aaccttgaag agcgaagttg gaggagacct agcaagtgca ccattggatt
     5101 tgacaaatca atctggtctt ctttattatc gtacccagtt gccaggcaag gaaattagta
     5161 atgtcctagc agtttccgtt ccaaaagatg acagaagaat caagtcagtc agcctagaaa
     5221 caggacctaa gaaaacaagc tacgccgaag gggaggattt ggaccttaga ggtggtgttc
     5281 ttcgagttca gtatgaagga ggaactgagg acgaactcat tcgcctaact cacgcaggtg
     5341 tatcagtatc aggttttgat acgcatcata agggagaaca gaatcttact ctccaatatt
     5401 tgggacaacc ggtaaatgct aatttgtcag tgactgtcac tggccaagac gaagcaagtc
     5461 cgaaaactat tttgggaatt gaagtaagtc aggaaccgaa aaaagattac ctagttggtg
     5521 atagcttaga cttgtctgaa ggacgctttg cagtggctta tagcaatgac accatggaag
     5581 aacattcctt tactgatgag ggagttgaaa tttctggtta cgatgctcaa aagactggtc
     5641 gtcaaacctt gacgcttcat taccaaggcc atgaagttag ctttgatgtt ttggtatctc
     5701 caaaagcagc attgaacgat gagtacctca aacaaaaatt agcagaagtt gaagctgcta
     5761 agaacaaggt ggtctataac tttgcttcat cagaagtaaa agaagccttc ttgaaagcaa
     5821 ttgaagcggc cgaacaagtg ttgaaagacc atgaaactag cacccaagat caagtcaatg
     5881 accgacttaa taaattgaca gaagctcata aagctctgaa tggtcaagag aaatttacgg
     5941 aagaaaagac agagcttgat cgcttaacag gtgaggttca agaactcttg gctgccaaac
     6001 caaaccatcc ttcaggttct gccctagctc cgcttcttga gaaaaacaag gccttggttg
     6061 aaaaagtaga tttgagtcca gaagagctta caacagcgaa acagagtcta aaagatctgg
     6121 ttgctttatt gaaagaagac aagccagcag tcttttctga tagtaaaaca ggtgttgaag
     6181 tacacttctc aaataaagag aagactgtca tcaagggttt gaaagtagag cgtgttcaag
     6241 caagtgctga agagaagaaa tactttgctg gagaagatgc tcatgtcttt gaaatagaag
     6301 gtttggatga aaaaggtcaa gatgttgatc tctcttatgc ttctattgtg aaaatcccaa
     6361 ttgaaaaaga taagaaagtt aagaaagtat ttttcttacc tgaaggcaaa gaggcagtag
     6421 aattggcttt tgaacaaacg gatagtcatg ttatctttac agcacctcac tttactcatt
     6481 atgcctttgt ttatgaatct gctgaaaaac cacaacctgc taaaccagca ccacaaaaca
     6541 cagtccttcc aaaacctact tatcaaccga cttctgatca acaaaaggct cctaaattgg
     6601 aagttcaaga ggaaaaggtt gcctttcatc gtcaagagca tgaaaatact gagatgctag
     6661 ttggggaaca acgagtcatc atacagggac gagatggact gttaagacat gtctttgaag
     6721 ttgatgaaaa cggtcagcgt cgtcttcgtt caacagaagt catccaagaa gcgattccag
     6781 aaattgttga aattggaaca aaagtaaaaa cagtaccagc agtagtagct acacaggaaa
     6841 aaccagctca aaatacagca gttaaatcag aagaagcaag caaacaattg ccaaatacag
     6901 gaacagctga tgctaatgaa gccctaatag caggcttagc cagccttggt cttgctagtt
     6961 tagccttgac cttgagacgg aaaagagaag ataaagatta aatatcgaaa aatcttgtga
     7021 aatctttcct tatatttcca aagtgtgata taatagtttc gaataaaata aataaaggag
     7081 tttttgtaac atggcaaaac ttactgttaa agacgttgac ttgaaaggta aaaaagtcct
     7141 cgttcgtgtt gacttcaacg taccattgaa agatggcgta atcactaacg ataaccgtat
     7201 cacagcagct cttccaacta ttaagtacat catcgaacaa ggtggacgtg caattctttt
     7261 ctctcacctt ggacgtgtga aagaagaagc tgataaagct ggtaaatcac ttgctcctgt
     7321 agcagctgac ttggcagcaa aacttggtca agatgttgtt ttcccaggtg tcactcgtgg
     7381 tgctgaatta gaagcggcaa tcaacgctct tgaagatgga caagttctct tggttgaaaa
     7441 cactcgttac gaagatgttg acggcaagaa agaatctaaa aacgatcctg aacttggtaa
     7501 atactgggca tcacttggag atggtatctt cgtaaacgat gcattcggta cagctcaccg
     7561 tgcacacgca tctaacgttg gtatctcagc aaacgttgaa aaagcagttg ctggtttcct
     7621 tcttgaaaac gaaattgcct acatccaaga agcagttgaa actccagaac gtccattcgt
     7681 ggctatcctt ggtggttcaa aagtttcaga caagatcggt gttatcgaaa acttgcttga
     7741 aaaagctgat aacgtcctta tcggtggtgg gatgacttac acattctaca aagcacaagg
     7801 tatcgaaatc ggtaactcac ttgtagaaga agacaaattg gatgttgcga aagctcttct
     7861 tgaaaaagca aatggtaaat tgatcttgcc agttgactca aaagaagcta acgcatttgc
     7921 tggttacact gaagtgcgtg acactgaagg tgaagcagtt tctgaaggct tccttggtct
     7981 tgacatcggt ccaaaatcta tcgccaaatt tgacgaagct ttgactggtg ccaaaacagt
     8041 cgtatggaac ggacctatgg gtgtatttga aaacccagat ttccaagctg gtacaatcgg
     8101 tgtgatggac gctatcgtga aacaaccagg agttaaatca atcatcggtg gtggtgactc
     8161 agctgccgca gcgattaacc ttggccgtgc agacaagttc tcatggatta gtacgggtgg
     8221 tggagcatca atggaacttc ttgaaggtaa ggttcttcca ggacttgcag ccttgacaga
     8281 aaaataagat tttataaata aatcaaagaa gagagggatg aaagttcctc ttttcttttg
     8341 cttaaaataa aaacgcttcc tctcaactat tactcataaa aatcaccgat ttatgataaa
     8401 atggaaatag aaagttgaga ttatgagtta ttttaaaaaa tataaattcg ataaatccca
     8461 gttcaaactt ggtatgcgaa cctttaaaac aggtattgct gtttttctag ttctcttgat
     8521 ttttggcttt tttggctgga aaggtcttca aattggtgct ttgacagccg tttttagcct
     8581 gagggagagt tttgatgaga gtgttcattt tgggacttcg cgtattctag gaaatagtat
     8641 cggtggactc tatgccttgg tcttcttctt attaaatacc tttttccacg aagccttttg
     8701 ggtgaccttg gtagttgttc caatctgcac catgttaacc attatgacaa atgtagccat
     8761 gaataacaaa gcaggggtta ttggtggtgt agcagctatg ttaatcatta ccctatcaat
     8821 tccaagtggt gagacaattt tgtacgtgtt tgtgcgtgta ttagaaacgt ttatgggagt
     8881 ttttgtcgca attatcgtaa attacgatat tgatcgtatt cgtctctttt tagagaaaaa
     8941 agaaaaataa tgttacattt tataacatta tcaattgacg tttgtctttt tttagactat
     9001 aatagacaga aagaaggaaa ttgtaaatga aggaaaaaga atttcgccga aatatggctg
     9061 tttttcctat cggcagtgtt atgaagttga ccgatctatc ggcgcgtcag attcgttatt
     9121 atgaagatca agagttgatc aagcccgatc gaaacgaagg aaatcgtcgc atgtattcct
     9181 tgaatgacat ggatcgtctg cttgaaatca aagattatat ctctgaaggt tataatatcg
     9241 ctgccattaa gaaaaaatat gctgaacgtg aagcgaaatc caagaaagcg gttagtcaga
     9301 ctgaagtacg tcgtgcactt cacaatgaac tcctccaaca ggggcgtttt gcttcagtac
     9361 agtcaccttt tggtcgcggt taggcaaccg caagtagtca tagattaagg agaaaactta
     9421 tgccaatcac agctgcagat attcgtcgtg aagtcaagga aaaaaatgtt acctttattc
     9481 gtcttatgtt ctcagatatt ttgggaacca tgaaaaacgt cgaaattcct gctacagatg
     9541 aacagttaga taaggtcttg tcgaacaagg ttatgtttga tggatcttct attgaaggtt
     9601 ttgtacgtat caatgagtcg gatatgtact tgtacccgga cttggataca tggacagtct
     9661 tcccttgggg agatgaaaat ggaagtgttg caggtctgat ctgtgatgtc tatacaacag
     9721 aaggtgaacc atttgcgggt gaccctcgtg gtaatttgaa acgagctctt cgtcacatgg
     9781 aagaagttgg attcaaatcc ttcaaccttg gtccagagcc agaattcttc ctatttaagt
     9841 tggatgaaaa tggggaccca acacttgaag tgaatgacaa gggtggctac tttgacttgg
     9901 cacctactga ccttgcggac aacacacgtc gtgagattgt gaatgtcttg accaaaatgg
     9961 gatttgaagt agaagcgagt caccacgagg ttgcggttgg acagcatgag attgacttta
    10021 agtacgatga agttctccgt gcttgtgata agattcaaat ctttaagctt gttgttaaaa
    10081 ccattgctcg caaacacgga ctttacgcaa catttatggc gaagccaaaa tttggtattg
    10141 ctggatcagg tatgcactgt aatatgtcct tgtttgatgc agaaggaaat aacgccttct
    10201 ttgatccaaa tgatccaaaa ggaatgcagt tgtcagaaac agcttaccat ttcctaggcg
    10261 gtttgatcaa gcatgcttac aactatactg ccatcatgaa cccaacagtt aactcataca
    10321 aacgtttggt tccaggttat gaagcgcctg tttacattgc ttgggctggt cgtaaccgtt
    10381 cgccacttgt gcgcgtacct gcttcacgtg gtatgggaac tcgtcttgag ttgcgttcag
    10441 tggatccaat ggcgaaccct tacgttgcta tggctgttct tttggaagtt ggtttgtatg
    10501 gtattgaaaa taaaatcgaa gcaccagctc ctatcgaaga aaatatctac atcatgacag
    10561 cagaagagcg caaggaagct ggtattacag accttccatc aactcttcac aacgctttga
    10621 aagctttgac agaagatgaa gtggttaaag ctgctctcgg agatcacatc tatactagct
    10681 tccttgaagc caaacgaatc gaatgggcaa gttatgcaac cttcgtttca caatgggaaa
    10741 ttgataatta tttagacctt tactaatact aatatagaag aaagattgcc tgtgggtaaa
    10801 cagtcacaca gcagtctaag ccatcaaata cctataaccc ttaaaatagc gatttcggtc
    10861 gctatttatt tttcgaaaaa tcctcttgac tcttttgcga attagattta tcatcagacg
    10921 tgtagaagaa aattaaggag tcactatgga aaaattcaat ttcaaaaaca atatcgggca
    10981 agaaaacaag ttacttcaaa tcgaaatcta caagtttact aacttttgca agcttcaaaa
    11041 ttacacatct gtaaatattt tttcgaaaga tatatttgaa gccatagtaa attgagacta
    11101 gaatagtaca ctacatctgc taaaaatttc tagaaactaa tttaactttc ctaatcgatt
    11161 tgtttacatc ttatttcaat ctactatatt atctcaataa atgatgatgg cgactgtttt
    11221 gtttgtgctt gtcgattttt gaatcctgat cgagcggaag aatatttctg ccgaggttgc
    11281 caaggggaaa atcctgaaga tattgaattt tatgatgaac aattacaggc agaaaaggtg
    11341 gaggatttga atattcgatt ggaagtaaaa aattgaatta tctacaatct catcaacaat
    11401 aaacctattt caaccccatt atctataggc gtatttttac gcctttttat atacttgaag
    11461 cacataagaa acattatggg aagtttgctg atggaagcac tca
//
LOCUS       DAVKNL010000059        11152 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVKNL010000059 DAVKNL010000000
VERSION     DAVKNL010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11152
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.059"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            <1..419
                     /locus_tag="VW411_001686"
     CDS             <1..419
                     /locus_tag="VW411_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1919 domain-containing protein"
                     /protein_id="HEU9794380.1"
                     /translation="HFRYYMEQELVFEEELISPSWTHEVYPLGHLGDIDIHFLHYSSI
                     EEARTKWEKRKKRINWNNLYFKFDNKDSASEEILNKMDNLPYSNKLILVNRPYKNLKS
                     QCVIPGQEHLPELAIMSDPLNTFDIMAWLKKGGNAL"
     gene            521..2650
                     /locus_tag="VW411_001687"
     CDS             521..2650
                     /locus_tag="VW411_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEU9794381.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATNRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRAQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYTAVKELFKRL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
     gene            2637..3086
                     /locus_tag="VW411_001688"
     CDS             2637..3086
                     /locus_tag="VW411_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEU9794382.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPIKLYRCQSCQQRYQRKRRIDTQRYRCGLCRGKLLLVNQPED"
     gene            3144..3416
                     /locus_tag="VW411_001689"
     CDS             3144..3416
                     /locus_tag="VW411_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU9794383.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(3770..3961)
                     /locus_tag="VW411_001690"
     CDS             complement(3770..3961)
                     /locus_tag="VW411_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794384.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFISTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            4389..5147
                     /locus_tag="VW411_001691"
     CDS             4389..5147
                     /locus_tag="VW411_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9794385.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFNEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            5149..7137
                     /locus_tag="VW411_001692"
     CDS             5149..7137
                     /locus_tag="VW411_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9794386.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTITYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQMNVVIAVLV
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKRGRFLPLQTILGSISLGIGYYLAL
                     TVTDPLTALTTFFLAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSAESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYSVKEKEVLRYSNFGIANQEGTKLTIFEKGQNRVQPTTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFNKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVNVSEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSNLADASSQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(7179..7823)
                     /locus_tag="VW411_001693"
     CDS             complement(7179..7823)
                     /locus_tag="VW411_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEU9794387.1"
                     /translation="MLRAGVLDANDGIISIAGVVIGVASATTNIWIIFLSGFTAILAG
                     AFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYAAYIQNGEFKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYR
                     IPATVLIVGVALLLTGYTSARLGKAPTKTAMIRNLAIGLLTMGVTFLLGQLFSI"
     gene            complement(7849..8844)
                     /locus_tag="VW411_001694"
     CDS             complement(7849..8844)
                     /locus_tag="VW411_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU9794388.1"
                     /translation="MQNHYTTKGEHLTIDSRRLIERWKKEGKSNREIASLLGKVPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVKKSSLTKELKEKILHYHNQ
                     KFSPEMMVMAKGVNVGISTIYYWIHHGKLGLSKQDLLYPRKGKALKKQASTNFKPAGQ
                     SIEQRLEAINFRLENGHYEIDTVLLTRAKNYCLLVLTDRKSRHQIIRLIPNKSADVVN
                     QALKLILKQHKILSITADNGTEFNRLSNVFSEEHIYYAHPYASWERGTNENHNRLIRR
                     WLPKGTKKMTPKEVAFIEKWINNYPKKCLDYKSPREDFLMANLNLKFSVRNKSRN"
     gene            9551..11026
                     /locus_tag="VW411_001695"
     CDS             9551..11026
                     /locus_tag="VW411_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU9794389.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTDKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSILLIGEQMNSEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEKVIELSEYARREINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDDSVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
BASE COUNT         3307 a         2119 c         2343 g         3383 t
ORIGIN      
        1 agcattttcg ttattatatg gaacaagaac ttgttttcga agaggaactt atttcaccga
       61 gctggactca tgaagtttat cctttggggc acttaggaga tatagatatt cattttctac
      121 attattctag tattgaagaa gcacgaacta agtgggaaaa aagaaaaaaa cgcataaatt
      181 ggaataatct ttattttaaa tttgataata aagattcagc tagtgaagaa attttaaata
      241 agatggataa tcttccttat tctaataaat taattttagt caatcgtcca tataaaaatt
      301 tgaaaagtca atgtgttatt ccaggtcagg aacatcttcc agaactggct attatgtctg
      361 atccattaaa tacttttgat atcatggctt ggttaaaaaa aggtggcaat gctctttaag
      421 tgcaagttat tgcgctgtag catataaatc tatttcctac atatttttta aatgttctac
      481 gagttaattt gaaacgttta gcttgtggta taatagattt atggataaaa aatatgaaaa
      541 aatctctcag gatttgggag tgacgttaaa gcaaattgat accgttctaa gtttgacagc
      601 tgaaggggcg actattccct ttatcgcgcg ttatcgcaag gacatgactg gtagtctgga
      661 tgaggtggcg attaaggcta ttattgattt ggataaaagt ctgacaaatc tcaatgaccg
      721 taaggaagct gtcttagcta agattcaaga acaaggtaag ttgaccaagg aattggaaga
      781 agctatctta gttgccgaaa aattagcaga cgttgaagaa ctctatcttc cttataagga
      841 aaagcgtcgt accaaggcaa ccattgcccg tgaagctgga ctctttcctc ttgctcgctt
      901 gattttgcag aatatagttg acttagagaa agaagctgaa aagttcgtct gtgaaggatt
      961 tgcgactggc aaggaagcct tgaccggtgc agttgatatt ttggtcgaag ccttatcgga
     1021 agatgtgacc ttgcgttcta tgacttatca ggaagtgctg agacactcta aacttacttc
     1081 tcaagccaag gatgaaagtc ttgatgaaaa gcaggttttt cagatttatt atgatttttc
     1141 agagacagtt ggaactatgc aaggctatcg taccttggct ctcaatcgtg gggagaaact
     1201 tggtgtcttg aagatcggtt ttgaacatgc gacgaaccgt attcttgcct tctttgctac
     1261 tcgtttcaag gtgaaaaatg cttatattga tgaagttgtt cagcaatccg ttaagaaaaa
     1321 ggtcttgcct gctatcgagc gtcgtattcg gacagaatta actgagaaag ctgaagaagg
     1381 agctatccaa cttttttctg acaatctgcg caatctcctc ttggttgctc cactgaaagg
     1441 gcgcgtggtt cttggatttg acccagcctt tcgtacaggt gccaagctag ctgtcgtgga
     1501 tgcaacagga aaaatgctga caactcaggt tatttatcct gttaaaccag catcagctcg
     1561 tcaaatcgaa gaagccaaga aagatttagc agatttaatt ggtcaatacg gtgtagagat
     1621 tattgccatt ggaaatggaa cggccagtcg tgaaagtgaa gcttttgtag cggaagttct
     1681 gaaagatttc cctgaagtca gctatgttat cgttaatgaa agtggtgctt ctgtctattc
     1741 tgccagcgaa cttgctcgtc aggagtttcc agacttgacc gttgaaaaac gctctgccat
     1801 ttctatcgcc cgtcgtttgc aagatcctct tgcggaattg gtcaaaatcg atcctaagtc
     1861 aattggtgtc ggtcaatacc aacacgatgt cagtcagaag aaactatctg agagtctgga
     1921 ctttgttgtc gatacagtgg ttaaccaagt tggtgtcaat gtcaatacag ctagcccagc
     1981 tcttctttca cacgtagctg gactcaacaa aactatctct gaaaatattg tcaaataccg
     2041 cgaggaagaa ggaaaaatca cttcacgcgc ccaaatcaag aaagttcctc gtctgggagc
     2101 caaggccttt gagcaggctg ctggtttcct tcgtatccct gaaagtagca atatccttga
     2161 taatacagga gttcacccag agaattacac tgcagttaag gaactcttca aacgtttaga
     2221 tattaaagac ctaaacgaag aagcccaaag caaactcaag tcgctttcag tcaaggaaat
     2281 ggcgcaagag ttagaccttg gtccagaaac tcttaaagat atcattgcag atcttctcaa
     2341 accaggtcga gatttccgtg attcctttga cgcacctgtg cttcgccaag atgtcctaga
     2401 tatcaaggac ttagtggttg gacaaaagct agaaggagtg gtgcgtaacg ttgttgattt
     2461 cggtgctttt gttgatatcg gaattcatga ggacggtctc atccatattt cccatatgag
     2521 tcgcaagttt atcaaacacc ctagtcaagt ggtggcagtt ggagatttgg tgagcgtttg
     2581 ggttaagaaa attgatactg aacgtgaaaa agttaatctg tctctcctag ctccaaatga
     2641 aactgactga ttacgtcaag caggtttcac tagaagactt cggcagacct tttatccatc
     2701 atgtccagtg gaataggcgt ctacgttcga caggtgggcg atttttcccc aaagatgggc
     2761 atttggattt taatcccaag gtttatcagg aactggggtt ggacgttttt aggaaaattg
     2821 tccgacatga actctgtcat tatcacctct attttcaagg gaaaggctat caacacaagg
     2881 atcgggattt taaggaactg ttgaaagcag tggatggatt acggtttgta ccatccttgc
     2941 ccaatagtaa ctccaagcca atcaagctct atcgttgcca atcctgccag caaaggtatc
     3001 agcgtaagcg taggattgat acccaacgct atcgctgtgg actttgtcga ggaaagttgc
     3061 tattagtaaa tcagcctgag gactgatgaa agccgagcct atccgtgata tactagtcta
     3121 acctagcaga aagaggaaat atcatgaaca gtaagtttta taaaatgaga cgaaatcgca
     3181 tgatttcagg agttttagca ggtctgtcag acaagtggaa ctttgatgtt acccttgtac
     3241 gttttctgtt tgccattttt acagtcgcaa actttggact tggggtgatt atctatatca
     3301 tcctagcttc tatcatgcca accaaggaag aaatcgaagc agaaatgtac ggaacaggcc
     3361 cacgtaaacg caaagaagct caagccattg acgataatga aggctggttt tggtgaggtt
     3421 ttacaaaaga tttggaatac gcaaaaagct ttaaatcaat agtctaaact gcttgattta
     3481 aagctttttt acttttatac tcaataaaaa tcaaagtgca aactaggaag ccagccgcag
     3541 gctgtaattt agtcggcaat gtgaagttga tatagtttgc actttgattt ttgaatagta
     3601 ttaataatat ttcgccagct tcatccacaa actatagtgg attggaacta gaataggaca
     3661 cctatacttc taaaacattg ttagaaatcg atttgactgt cctgatcgat ttgtcctgtt
     3721 cttatttcat tttactataa aagcagtaat ttaaacgcag ttttttaatc tatttttgct
     3781 gttgagacaa tacatttttg gaaaaatggt ggatgctgat aacaactgat aaccagcaaa
     3841 taacataagc agtgccggta aattctgtac tgataaagtg tgtgtgttca actaagagct
     3901 gaccgatgaa tggagagagc agatagagaa gtccgatgag tatatgttct ttgtgtttca
     3961 ttaaagcgca ccttctttcc ttataggttg aacgacttgc aagtgttaaa ttgaatggta
     4021 tgttatctag gtaagcttac taaaatgtta gttcccgctt cctccttttc aaagtaagcc
     4081 ttttcacact tatattttac aactttaaaa ataggaaatc aaatcatttt ctaagtaatt
     4141 ttgcttatat ttcacccaat cttacaaaaa tgtaagatta aagtctcttt tgtaaggtta
     4201 ggttatggca agcggtcttt ttttgatgta aactagactc ataaagaaca aaggagtaac
     4261 atcatgaaac aaatcttaca taagaaaact agaaaaccaa gtcacaaaga aattgagcgc
     4321 gttcagctgg gatgcactat gatgcaagct acatttcatt tgatgggata ttaagaaagg
     4381 agattttcat gacactttta gatgtaaaac acgttcaaaa aatttataaa acacgttttc
     4441 agggcaacca agtagaagcc ctcaaggata ttcactttac cgtagaaaag ggtgactacg
     4501 ttgccatcat gggtgagtct ggttctggta aatcaactct tctcaatatt ctagctatgt
     4561 tggataaacc aagtcgtggt caggtttact tgaatggaac tgacaccgca actattaaaa
     4621 attcacaggc ttctagtttc cggcgtgaaa agctaggatt tgtcttccaa gactttaact
     4681 tgctagatac tctgtctgtt aaggacaata tcttgcttcc gcttgtcttg tcaagaagac
     4741 ctataacgga gatgatgaag aaattggtgg tgacagctga gaatctgggt attaaccaat
     4801 tgcaagagaa gtacccttac gagatttctg gtggtcagaa acagcgtgta gcagtagccc
     4861 gcgccatcat cacagaacct gaaattctcc ttgcggacga gccaacagga gcccttgatt
     4921 ccaagtcatc tgcagcctta cttgatgtct ttaatgaaat caatgagcgt gggcaaacca
     4981 tcctcatggt aacccactca acagcagctg ctagcagggc caagcgtgtt ctctttatca
     5041 aagacggcat tctttacaac caaatctacc gtggagagaa gacagagcgt cagatgttcc
     5101 aagaaatctc tgataccttg actgtcatgg caagcgaggt gaattagtat gtttcgatta
     5161 accaataagt tagcggtatc gaacttgatt aaaaaccgca aactctacta tccctttgca
     5221 ctggctgttc tcttggcagt caccatcacc tatctctttt actccctaac cttcaatcca
     5281 aagattgcgg aaatccgtgg aggaaccacc attcaagcaa cacttggatt tggtatgttt
     5341 gtcgttaccc ttgcgtcagc cattatcgtc ctctatgcca atagttttgt catgaaaaac
     5401 cgttccaagg aactgggtat atatggcatg ttaggcttgg agaagcgcca tctaatcagt
     5461 atgaccttta aggagttagt ggtatttggg attctaactg ttggagcggg tatcggtatt
     5521 ggagccttgt ttgacaagtt aattttcgct ttcctgctca aactaatgaa actgaaggtt
     5581 gagctggttg ctaccttcca aatgaatgtt gtcattgcag tacttgttgt ctttggattg
     5641 attttcctag gcctcatgtt cctgaatgct cttcgaatcg cccgtatgaa tgccctccag
     5701 ctctcgcgtg agaaagcaag cggagagaaa agaggtcgct tcctacctct ccaaacgatt
     5761 cttggttcca taagtttagg gattggctat tatcttgccc ttacggtaac cgatcctctt
     5821 acagccctaa caactttctt cctagctgtt ttgctggtta tctttggtac ttatctattg
     5881 tttaatgcag ggattacagt cttcctacaa atcttaaaga aaaacaagaa atactattac
     5941 caacctaata acctcatatc tgtttccaac ttgattttcc gtatgaagaa aaatgcggtt
     6001 ggactagcaa ccatcgctat tttgtcaaca atggttttgg taaccatgtc agcagcgaca
     6061 agcattttca attccgcaga aagctttaaa aaagttctaa atcctcatga ttttggggtt
     6121 tcagggcaaa atgttgaaaa agaagatttg gacaaactct tgagccagtt tgcaagtgac
     6181 aaaggttata gtgtcaaaga gaaagaagta cttcgttaca gtaactttgg tattgcaaat
     6241 caagaaggaa ccaagttaac tatttttgaa aaaggacaaa accgtgtcca acccacaaca
     6301 gttttcatgg tatttgacca aaaagattat gaaaatatga ctggtcaaaa actgtctcta
     6361 tcaggaaatg aggtcggtct ctttgccaaa aatgacggac tgaaaggaca gaaagctcta
     6421 actctaaatg atcatcaatt ttctgtcaaa gaagaattta ataaagattt cattgtgaac
     6481 catgttccaa ataagtttaa tatcttgact actgattaca attaccttgt tgttcctgat
     6541 ttacaagcct ttttggatca attcccagat tcggctatct ataatcagtt ttacggtggt
     6601 atgaatgtaa atgtcagtga agaagaacaa ctcaaggtcg ctgaggagta tgaaaactac
     6661 ctcaatcaat ttaatgctca attagacaca gaaggtagct atgtttatgg tagcaatcta
     6721 gcagatgcta gttctcagat gagtgccctc tttggtggtg tcttctttat cggtattttc
     6781 ctatccatta tctttatggt cggaactgtt ctggtcatct actacaaaca aatttctgaa
     6841 ggctacgaag accgtgaacg ctttattatc ttgcagaaag tcggtttgga ccaaaagcaa
     6901 atcaagcaaa ccatcaacaa acaggtttta actgttttct tccttccttt gctctttgcc
     6961 ttcatacatc tcgcctttgc ctaccatatg cttagcctga ttttaaaagt gattggtgta
     7021 ctggatacga ctatgatgtt gattgtgacc ttgtctatct gcgctatctt cctcatcgcc
     7081 tatgtgctga ttttcatgat tacttcaaga agttatcgca agattgtgca aatgtaaaaa
     7141 agatacctcg acttcaaaat cgaggtattt cttgtattct aaatgctgaa aagttgtccg
     7201 agcaggaagg taactcccat ggtcaagaga ccaatagcaa ggttccgaat catagctgtt
     7261 ttggttgggg cttttccaag tctagcactt gtgtaaccag tgagaagaag ggccacaccg
     7321 acaataagga cggtagcagg gatgcggtaa tcacttggaa aaatggtcac tgacagcatt
     7381 ggaggcaaac ttctaaggaa aaaggcaacg aagctagaaa tggcagcgtg ccaaggattg
     7441 gtaaattctt catactcaat cccatatttt tcctctacca gagccttgag tggattttta
     7501 agaaagatct tattggtcaa gagttgggca gaagttttga attctccatt ttggatataa
     7561 gcagcataga gggatttttt ggctagttcc ctatcttggt ctagcaagag tttttctcgc
     7621 gaaacggcag cttcctcggt atcttttgga gttgaaacgg atacatattc tccaccagcc
     7681 attgaaaagg caccagctaa gatagccgta aaacctgata aaaagataat ccagatattg
     7741 gtcgtggcac tggcaactcc gataaccaca ccagcaatgg aaataattcc atcgttagca
     7801 tcaagaacac ccgcacgcag gatatttaaa cgacctgcaa aatttgaatc aatttcgtga
     7861 tttgtttctg acgctaaatt tcaagttcaa gttagccatc aagaagtctt ctctgggtga
     7921 cttgtagtcc aagcattttt taggatagtt gttaatccac ttttcgatga atgcgacttc
     7981 tttgggagtc attttcttgg ttcccttagg taaccatcta cgaatgagcc tgttgtgatt
     8041 ctcattagtt cccctttccc aagaggcata gggatgtgca taataaatgt gctcctcaga
     8101 aaatacatta gacaagcgat tgaattccgt tccattatct gccgtgatgg aaagaatctt
     8161 gtgttgtttt aagatgagtt ttagagcctg attgaccaca tcagcacttt tatttggaat
     8221 caatcggatg atctgatgtc tactttttcg atccgtcaag acaagcaagc agtagttttt
     8281 cgctctcgta agtagaactg tatcaatctc ataatgccca ttctccaagc gaaaattgat
     8341 agcttcaagc cgctgttcga tggattgacc agcaggttta aagttggtgc tggcctgttt
     8401 cttaagcgct tttccttttc tagggtaaag cagatcctgt ttgcttaacc ccaattttcc
     8461 atgatgaatc caatagtaaa tggttgaaat tcccacgtta acccctttag ccatcaccat
     8521 catttcaggc gaaaattttt ggttatgata gtggagaatc ttttccttta gttccttggt
     8581 caagcttgat ttcttgaccg agcgcttgcg attgttttca taagactgtt gagcatagtc
     8641 ggcagaataa acctctttga agcgcccttt tccaagacat tgtcggactg tcccacgctt
     8701 gatttcagtg tggatagttt gaggaacttt tccaagcaga gaggcaattt ctctatttga
     8761 tttcccttct tttttccatc tttcgattaa gcgacggcta tcgattgtca aatgttcgcc
     8821 ttttgtagta taatggtttt gcatctctgt gcctttcttg tgtttgtggt tgaacaacaa
     8881 gtataacaca gaggtgtttt cttatgccta caagagctat cggctagttg aaccatctaa
     8941 tttttaggag ggctgggtgg ctaacttcat tatagaactt tcatttacga acatatagta
     9001 aaatgaaaca agaacagaac aaatcgatca ggacagtaaa atctatttct aacaatgttt
     9061 tagaagcaga ggtgtactat tctagtttca atctattata tttttgtttt ttatcaaaaa
     9121 atactttaca agttcttaaa aacatgatat agtaataaag cttagaaaat gagatgatgt
     9181 tttctagcaa atataaaccc gagtaaaaaa tgcctacgga caggcagggt tgaatgccga
     9241 agcgtggttg aaaagccaca ttattgatag ggttaaaagc ctacttttat aagttgatgt
     9301 taggacactt gtcctaattc ataaattttt agtgtggtga aagcacacgt catcttgtga
     9361 aacgatcaat aaagtacgta atatttgcta ctagagagtt aggaaacatc gggaacagac
     9421 atactcaaca gaaaccaaaa taaacacgtc agaagattgc agagcaggtg aaaacctgct
     9481 cttttttcat gagtcaacct ttagttcctt agttttcata aggtcctaaa aatattgaaa
     9541 ggagtatgtt ttgaaagagt tagatcaaaa ccaagcccca atttatgagg ccttggtgaa
     9601 gttacgcaag aaaaggattg ttccctttga tgttccaggt cacaagcgtg gacggggaaa
     9661 tccagaactt gtcgaactct taggagaaaa atgtgtaggc attgatgtca attcgatgaa
     9721 acctttggat aatttaggcc atcctatttc gattattcgt gatgcagagg agctggctgc
     9781 agatgctttt ggagctagcc atgcctttct aatgattggt ggaacaactt catcggtgca
     9841 gactatgatt ctggcaacct gcaaggcagg agataagatt attctgccac gaaatgtcca
     9901 taaatctgct atcaatgcgt tggttctatg tggtgccatt cccatctata tcgagatgag
     9961 tgtagatcct aagattggta tcgctttagg tcttgaaaat gaccgagtag cacaggccat
    10021 aaaggaccat ccagatgcta aggctatcct aatcaacaat cctacttact acggcatctg
    10081 ttcagaccta aaggggttga cagaaatggc tcatgaagct ggcatgatgg ttttagtaga
    10141 tgaagcccac ggagcgcatt tgcatttcac tgataaactt ccaatttctg ctatggatgc
    10201 aggggctgat atggcagcag tttccatgca taagtctggt gggagtttga cccaaagctc
    10261 cattttactt atcggggagc agatgaattc tgaatacgtt cgtcagataa ttaacctgac
    10321 ccagtctaca tctgcctctt acttgttgat ggctagtttg gatatttcac gtcgcaactt
    10381 ggcccttcgt ggtaaagagt cgtttgagaa agtcattgag ctatctgagt atgcccgccg
    10441 tgaaatcaat gctatcggtg gctactatgc ctactcaaaa gagttaatag acggtgtttc
    10501 ggtttgcgat tttgacgtaa ctaagctgtc agtttacact cagggtattg gcttaacagg
    10561 tatcgaggtt tatgacctct tgcgagacga atacgacatt cagatcgagt ttggtgatat
    10621 cggcaatatc ttggcctata tttccatcgg cgaccgcatc caagacatcg agcgcttggt
    10681 tggtgctctg gctgatatta agagactcta ttcaagagat ggaaaagatt tgatagcagg
    10741 agaatatatt cagcccgagt tagtgctgtc tccgcaagaa gccttctatt cagaaagaaa
    10801 aagtttaact ttggatgatt ctgttggaca ggtctgtgga gaatttgtta tgtgttaccc
    10861 tccaggtatt cctatcttgg ctcctggtga acgcattaca cgagaaattg tcgactatat
    10921 ccaattcgcc aaggaacgtg gttgctccct ccaagggacg gaagatccag aggtcaatca
    10981 tatcaacgtt attaagagaa agacaaacta taagaaaagt caatagtttt atctaaacta
    11041 tttcttattt caatttgatg atttggcgat gattttagag cacggcaaaa agcccttgaa
    11101 ttagaagcgg tcaatcgctt aatttctatc agcttatcaa atcctgcctc aa
//
LOCUS       DAVKNL010000060        11105 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVKNL010000060 DAVKNL010000000
VERSION     DAVKNL010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11105
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.060"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(185..257)
                     /locus_tag="VW411_001696"
     tRNA            complement(185..257)
                     /locus_tag="VW411_001696"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(222..224),aa:Lys,seq:ctt)
     gene            complement(522..1793)
                     /locus_tag="VW411_001697"
     CDS             complement(522..1793)
                     /locus_tag="VW411_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEU9794390.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYTARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQKIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
     gene            2091..2306
                     /locus_tag="VW411_001698"
     CDS             2091..2306
                     /locus_tag="VW411_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794391.1"
                     /translation="MMELPSQEILIFTKQIRHWILSDQVISGKRKLFFREDTPKEILD
                     LYENIKSKLDFAYQEVHSNNGLKKYEK"
     gene            2471..2650
                     /locus_tag="VW411_001699"
     CDS             2471..2650
                     /locus_tag="VW411_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794392.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSVPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            2787..2966
                     /locus_tag="VW411_001700"
     CDS             2787..2966
                     /locus_tag="VW411_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEU9794393.1"
                     /translation="MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERPITILHGE
                     LNKYTERGIRKQAGL"
     gene            3003..3455
                     /locus_tag="VW411_001701"
     CDS             3003..3455
                     /locus_tag="VW411_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEU9794394.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            3746..4216
                     /locus_tag="VW411_001702"
     CDS             3746..4216
                     /locus_tag="VW411_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEU9794395.1"
                     /translation="MATYGFLDILEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDVLMDFLEDPEAEEFVMEWNQEVFEEGKIGLEKGEFYPYPRY"
     gene            4235..5323
                     /locus_tag="VW411_001703"
     CDS             4235..5323
                     /locus_tag="VW411_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU9794396.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAILFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQLKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENWLAELEKEMLEGTEDGTRNF"
     gene            5304..5732
                     /locus_tag="VW411_001704"
     CDS             5304..5732
                     /locus_tag="VW411_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9794397.1"
                     /translation="MELEISDFTGCKIALFCGDKLLTILRDDKASIPWANMWELPGGG
                     REGDESPFECARREVYEELGIHLDEDCLLWSKIYPSVIFKGKKSVFMVGQLRQEQFDN
                     IIFGDEGQGYQLMNVEEFLSSSQVVPQLQERLKDYLKVSD"
     gene            5868..6818
                     /locus_tag="VW411_001705"
     CDS             5868..6818
                     /locus_tag="VW411_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEU9794398.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARLAELTDFMDLGELKIGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEMIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNPG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLIDDAYRLVKDEGYLIMSGIIKDKL
                     DMVRQSAESAGFFLETHMVQGEWNTCVFKKTKDISGVIGG"
     gene            6820..7563
                     /locus_tag="VW411_001706"
     CDS             6820..7563
                     /locus_tag="VW411_001706"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEU9794399.1"
                     /translation="MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDSIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     AGLGPRILRAETAPLYALSALSVLVELEK"
     gene            7574..9370
                     /gene="pepF"
                     /locus_tag="VW411_001707"
     CDS             7574..9370
                     /gene="pepF"
                     /locus_tag="VW411_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU9794400.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYGFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVAADEEVLDRLGKLPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDATLTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKHSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIISYSAELGE"
     gene            9616..10170
                     /locus_tag="VW411_001708"
     CDS             9616..10170
                     /locus_tag="VW411_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEU9794401.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLFDHLGNPSPEIRNVLLNQGL
                     HYLSKEKDTRGFSSQYGWVHAFAHGADLLTEVVCHPDFPKNRVHEVFDILGQLFKRMS
                     IRFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVDFPIEEREDFYKFSNFRSCLVE
                     VYVQLDQRNSLQDDLKEAIQSFQY"
     gene            10281..10949
                     /locus_tag="VW411_001709"
     CDS             10281..10949
                     /locus_tag="VW411_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEU9794402.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLAN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
BASE COUNT         3211 a         2016 c         2640 g         3238 t
ORIGIN      
        1 attttaggac ataaagatat ctccatgttg attgacgttt atgggcatac cttggaggaa
       61 aaaattagtg aggagtttat ggctgtgaaa agtttgttgt aaaaacacgg caaattgcac
      121 gacaattttt aaaaggaaag caaaaaaagc cctatattat aaggcttttc ctgttgtatt
      181 tatatgcccc ctacagggat cgaacctgtg acccacggat taagagtccg ctgctctgcc
      241 agctgagcta aggaggcaaa gaaaaaagct gtattggtgc cgaaacttca cggtttgtat
      301 tgaacccgcg caattaagca ggtgggcaac tcgctctaac tgaagctgtt tccgtgtgag
      361 acggcctaca tgctgttaga agacttttgt ttccctaata atacaaaaaa tagtcggtca
      421 acacttaagt gtgaagtcgt acaccacagc gtttctatat ttatatgata ccactttttc
      481 aaaaaaaatc aagaggaaag tgcaattttt tgaaaaggat ttcagatttt tcgcaaacgg
      541 tcgatagctt cctttctttg agccaaagcc cgttcatatt taccagtatc ttctgcttgg
      601 aaatagtgat gattacgaat tttttctggc agatagtctt gcttgaccca atttccagga
      661 tagttgtgtg gatagagata gtcttgggca ttccccagtt ccttgcttcc actgtagtgc
      721 ccatcacgca ggtgtcgcgg aataggcaag tgccctgatg ttttgaggtc agcaagtgcc
      781 ttatccatag ctacataggc tgagttggat tttggagaaa gggccaaatc aatcacgaca
      841 ttggcgatga gaatgcgggc ttctgggaac ccaatcttct gggcagcatc cagagcagtc
      901 acggtatgaa tctgggcctc aggattggct aagccgatat cttcataggc gataacagtc
      961 aagcgacgag cgagactagg cagatcccca gcctcaatca agcgggcagt atagtggaga
     1021 ctggcatcca catctgagcc acgaatagac ttttgcaggg cagatagaac atcatagtga
     1081 ccgtctccat ccttgtccat agtgatgtag cttctctgaa gactattttc catgatgtct
     1141 aaggtgatgt ggcgaatgcc ctcgtcattc tcaggggtag agagaacagc taagtccagt
     1201 gagttaaagg cagagcgaag gtctccgttt gtagaggttg cgatgaaatc cagcgcatcc
     1261 tcatctagtt ctattggaaa atcaaaacca cgttcagggt tacttagagc tatctgcagg
     1321 gcctctttga cgtcttggtt agacagaggt tccaactcga aaatttgaac tcggctacga
     1381 atggcaggag tgacagagaa gaaaggattt tcagtcgttg ctccaatcat gatgaccagt
     1441 ccactttcca agagaggcaa gaggaagtct tgcttggtct tatctagtcg atgaatttcg
     1501 tctagtaata ggacgagacc accagaaaat ttagcttctt ccgagatttc ttgcagtcgc
     1561 tttttactat caactgtcgc attgaaagtt cgaaaggcat acttggtcgt tccagcgatg
     1621 gcagaggcaa tactggtttt gccgattcca ggagggccat atagaatcat ggaggacaag
     1681 cggttggctt ccaccatgcg gcggatgatt tttccaggtc cgaccagatg ctcctgaccg
     1741 atgacctggt cgatggtttt agggcgcatg cgaagcgcga gattgtctgg catagcagtc
     1801 ctttctaacg tggatttgag tagaatgact catctactat atcttactat gtccattcta
     1861 gcctattagt tgtgatattt caatggtttt gtaataatca gggagtgaga tagaatcttt
     1921 tttactattt aatttaatga ggagaggtat cagcatgaga gatttgattt tccttaaagc
     1981 tcaaaatagg atagttgagc ctaaagaagt ggaagtgctg acttgagaaa taatattgct
     2041 gaatgtcatt gtatgaacta taatgaaaag agaggtgata agcaatggaa atgatggaac
     2101 taccaagtca ggaaattttg atttttacaa aacaaattcg tcattggatt cttagtgatc
     2161 aagttatttc agggaaaaga aaacttttct tccgggaaga tactccaaaa gagattttag
     2221 atttgtacga aaacattaaa tctaaacttg attttgctta ccaagaagtt cattctaata
     2281 atgggttgaa aaaatatgaa aaatagaata attgatgttt ttgaagtagt aaatcgcctc
     2341 cttgtaataa ctgttgaaaa tcctgatttc gaagacttga gagttaatca tactcaatga
     2401 aaatcaaaga gcaaactagg aaactagccg caggctgtac ttgagtacgg taaggcgacg
     2461 ttgacgtggt ttgaatttga ttttcgaaga gtatcagttt gtaaaaattg gagataaaaa
     2521 atatagagta cgcagtgttc ctatgattca ttcaactccg cctcaatctg tcttagatcg
     2581 agatactttt acaattgact atacagatga tgaattgctc gataaagaag cagtttttac
     2641 tactcattag aaaaataggt atgaataaat tggcttccct tagaaccaca tctaggggct
     2701 tttatgtata atagaataat aagtatcact cctttacaat acgtgtaaca cgtgttataa
     2761 tggtgttgtc aggaggtaaa agcgctatgc ctatgacaca aaaagagatg gttaaactcc
     2821 ttacggccca tggttggata aagactagag gcggtaaagg ctcccatatt aaaatggaaa
     2881 agcaagggga aagacctatc acaatccttc atggtgagct gaataagtac actgaaagag
     2941 ggataagaaa gcaagctggg ttgtaaaatt cagtccctgc tttttgatat ggaggtagtg
     3001 aaatgttagt tacgtatcca gctttatttt attatgacga cacagacgga acagaagcga
     3061 cttattttgt ccatttccca gattttgaat actcagctac acaaggagag gggatttctg
     3121 aggctttggc tatggggtcg gagtggctag ggataactgt tgcagatttg atagagagtg
     3181 atggagagtt acctcagcca tcagatatca atagcttgtc tttaattgat aatgatcctt
     3241 ttaaagatga tgaagatttc gtgtcaacct atgaccttga taaatctttt atttctatgg
     3301 tatctgttga tgtatcagaa tacctaggaa gtcaggaacc cattaaaaag actttgacca
     3361 taccaaaatg ggcagataag ttgggacgag aaatggggct taacttttct cagactttga
     3421 cagacgctat tgcagataag aaagttcaag cctaagatta agcgtggtat cgtggtaata
     3481 gtcaataaaa aagcacgtcc acttgtgcta gttactcgcc tattgatttt atagatttat
     3541 tagccctttc gagggctttt tatattgatt tttatgagaa atgaagaaat cagactttta
     3601 agaaaatcac tgataccaag ggtttaaatg aggtaatatg gtataattag gacataaaat
     3661 aattttgtgg taagatggta gtatctattt tagcatattt ccgagcaatc ggggcgatta
     3721 aagagtcgca tagaaagagg acaaaatggc aacatacgga tttttagata ttttagagga
     3781 agagttggac aagaactttc cctttgactt tgagattagc tgggacaaac gcaaccatgc
     3841 ggtagaagtg agttttctat tggaagcgca aaatgctgca ggtgtggaga tggtggatga
     3901 agacggagag gtttcatcag atgacattct ttttgaggaa gcggtccttt tctacaatcc
     3961 tgccaagtca acagtcaatg aggaagacta tttgactgtt attccttacc tgcctaaaaa
     4021 aggattttcc cgtgaatttt tagcttattt tgcgcttttc cttaaagata ctgctgaggt
     4081 tgggctagat gtcctcatgg actttttgga agacccagaa gcagaggaat ttgtcatgga
     4141 atggaaccaa gaagtctttg aagaaggaaa aattggcttg gaaaagggag aattttatcc
     4201 ttatccaaga tactaggagt tggttggagg tgttatgaag aaaattggga cttatttggt
     4261 ttatgtgcta gctgttgtct ttattatgct agcttttgct tgtggaacaa tcgcatttgc
     4321 agagttgggg tattccgcag ttttagcctt tacttttggt tatgccttcg ctcttctaag
     4381 catgtattta atctttattc ttcatgagct gggtcatgct ttttgtggct acttgacagg
     4441 ctatcggctg gtggcttttg gattaggaca ttttattttg accaaaaagt caggcaggtt
     4501 tcatcttagt agaacagcca ttctgaaaaa tgttggtgct caatacattg gtttaaaaga
     4561 ggatgaaagc gatcaaagaa tcatcctgat gctttcagga ggcttgatgg ttcatctcgg
     4621 cttgatatta ttggcgatat tgtttggatt tttgacaaga agctggtatt ttgcagggac
     4681 ttggattttt cttaatttat ctttcttcct aaataacatt ttgccagtcg acatcaccga
     4741 tggagcaaaa atttgggaat tactacaaca ccctgaaaat accaaatacg cctacctggt
     4801 attgagacat tctgcccaga ccttgctagc tcctcaagaa tatgatttga aagactttgt
     4861 tcaagcagtt cctgaggatg cacgagggag ttttgcggat ggtgtattag gtatgcaagg
     4921 tgaagtttcc attttggaag ggaaggaaga agtagctaaa cagcagtttc aagcattact
     4981 tgagaggaca actactccga tgatgcagac agttgcccaa ctatccctgc ttcatatcgc
     5041 cttgttggaa ggagattttg agcaagcgga gcaatatgct agtattcgac aggtcaaatc
     5101 gtttctgtct ctcaaactgt ccaatcttca agtgatacaa gcctggtatc aattaaaggt
     5161 gaagaaagat gtagatcgaa ctcgtaaggc tctcaagatt gctagacaga aaatggatag
     5221 tagtcgaatg ttgagggatg agaaaagata ttatgaaaac tggttagccg agctggaaaa
     5281 ggaaatgtta gaaggaactg aagatggaac tagaaatttc tgatttcaca ggttgcaaga
     5341 ttgctttgtt ttgtggggat aagcttttga ctatcttacg cgatgataag gcaagtattc
     5401 cctgggccaa tatgtgggaa ctgccaggtg gtggccgtga aggggacgaa agcccttttg
     5461 aatgcgctcg gcgtgaagtt tatgaggaac tgggaattca tttagatgaa gactgtctgc
     5521 tttggagcaa gatttatcct agcgtaattt ttaaaggcaa gaaatcggtc tttatggttg
     5581 gtcagctaag acaagaacaa tttgacaata tcatcttcgg agatgaagga cagggctatc
     5641 agctgatgaa tgttgaggaa tttcttagtt ccagtcaagt tgtgcctcag ttgcaagaga
     5701 gattaaaaga ttatttaaaa gtaagtgatt agaaaggatg gttcagttac atttctaaac
     5761 tgaacccgcc ctaaacactg tgccaaaaag ataaacttct cttagacaca agcgtcttca
     5821 gagaatttcc tattttggtt ttgtgtttta cgggcttggt attaataatg gaaacatggc
     5881 aagagttaaa agttacagtg aagcgtgagg gagaggagtt ggtttccaat ctcttgattg
     5941 agctgggagc gcaaggagtc gcaatcgaag acagtatgga ctatgtggga aatgtggacc
     6001 gctttggaga gattttccca gaggtggagc agcaagaaga aatcgtagtg acagcctact
     6061 accctgacac ggtggatgtg acagtggttg aggcggattt gcaggctcgt ctagcagaat
     6121 tgacagattt tatggatttg ggagagttaa aaataggtac gactgccttg gctgaggaag
     6181 actgggcaga caactggaag aaatactatg aacctgctcg tatcactcat gatttgacca
     6241 tcgttccctc ttggacagac tatgaggcga ctgcggggga aatgattatc aagctggatc
     6301 ctggcatggc ttttggtact ggaacccacc caactaccaa gatgagcctt tttgccttgg
     6361 aacaggttct tcgtggtggc gaaacggtgc tagatgtggg gactggttca ggggttctct
     6421 ctattgccag ctcgcttctg ggggctaaag aaattttcgc ctatgacctg gatgatgtgg
     6481 cggttcgtgt ggctcaggaa aatattgagc tcaaccctgg catggaaaat atccatgtag
     6541 cggctggaga tttgcttaag ggtgtggaaa ttgaggcaga tgtgattgtg gctaatatct
     6601 tggcggatat cctcattcat ctaatagacg atgcttatcg cttggtaaag gatgaaggct
     6661 acctgatcat gagtggcatt atcaaggaca agttggacat ggtgcgccag tcggctgagt
     6721 cagctggatt tttcctcgaa actcacatgg ttcaagggga atggaatacc tgtgtcttta
     6781 agaaaaccaa ggatatctct ggtgtgattg gaggctagca tgcagcagta ttttgtaaaa
     6841 ggcagtgcta tctctcctgt cactatcgag gacaaggaaa ccagtaagca tatgtttcag
     6901 gttatgcgct tgaaagaaga ggatgaggtt accttagtct ttgatgatgg catcaagcgc
     6961 ttggcgcgcg tgctggatat ggaaaatcgt cagtttgagt tggtccaaga attaaatgac
     7021 aatgtggaac taccagtcca agtgaccatc gcatctggat ttcccaaggg agacaagctg
     7081 gagttcatcg ctcaaaaagt aaccgaactg ggggctagcc aaatctgggc ctttcctgca
     7141 gactggtcag ttgccaagtg ggatggcaag aaattgggta aaaaagttga aaaactagaa
     7201 aaaattgccc ttggagcagc cgagcaaagc aagcgtaata ttgtaccaag tattaaactt
     7261 ttcgagaaaa aagcagattt tctagctcag ctggaccagt ttgactctat catagtagcc
     7321 tatgaagaat cagctaaaga aggagaagcc gctgcgcttc tgcaagcagt cactggtctt
     7381 gaaacaggag ccaaactgct ctttatcttt ggtccagaag gcggtctctc acctgcagaa
     7441 atcgaaagtt ttgaagctaa aggagccgtt ttggcaggcc taggtcctcg tattttgcga
     7501 gcagaaacag caccgcttta cgctttatca gcccttagtg ttttagtaga attagagaaa
     7561 taagaggaag aaaatggaac aaaaacaccg ttcagaattt ccagagaagg aactctggga
     7621 cttaacagcc ctataccaag accgtgagga tttcttgcgt gcaatcgaga aagctcgcga
     7681 agacatcaac cagtttagcc gtgattacaa gggcaatctt cacacttttg aggatttcga
     7741 gaaggccttt gcggaattgg aacagatcta cattcagatg agccatattg gcaactatgg
     7801 ttttatgcct cagacgacgg actatagcaa tgacgaattt gccaatattg cccaagctgg
     7861 gatggaattt gaaacagatg ccagcgtagc cttgaccttc tttgacgatg ccttggtggc
     7921 agcagatgag gaagtcttgg accgtttggg taaattgcca catttaacag ctgccattcg
     7981 tcaggctaaa atcaaaaaag cccactactt aggggcagat gtggagaagg ccttgacaaa
     8041 tctcggtgaa gttttctaca gtccgcagga catttatact aagatgcgag ctggggattt
     8101 tgaaatggct gactttgaag cccatggcaa gacctacaaa aacagctttg tgacctatga
     8161 gaatttctac caaaaccatg aggatgctga ggttcgtgag aaatccttcc gttccttctc
     8221 agagggactt cgtaagcacc aaaatacggc tgcagcagcc tatctggctc aggtcaagtc
     8281 tgaaaaactc ttggctgata tgaagggata cgactctgtc tttgactatc ttctagctga
     8341 acaagaagtg gaccgtgtca tgtttgaccg ccagattgac ctcatcatga aggactttgc
     8401 accagtcgct cagagatacc tcaagcatgt tgccaaggta aatggtcttg aaaagatgac
     8461 ctttgcagac tggaaattgg acttggacag cgccctgaat cctgaagtga ctattgacga
     8521 tgcctatgat ttggtcatga agtcggtaga acctttgggg caagaatatt gtcaggaagt
     8581 tgctcgttac caagaagagc gctgggtgga ctttgctgct aacagtggca aggattccgg
     8641 tggttatgcg gcggacccat atcgcgtaca cccttatgta ctcatgagct ggacaggccg
     8701 tttgagcgat gtctatacct tgattcatga aatcgggcat tctggtcaat tcatcttttc
     8761 agacaatcat caaagttact tcaatgccca tatgtcgacc tactatgttg aagcaccgtc
     8821 aaccttcaat gaattgctac tcagtgatta cttggagaac cagtctaatg acccacgtca
     8881 aaaacgcttc gctctggctc atcgcttgac agacacctac ttccataact ttatcaccca
     8941 cctcttggaa gccgccttcc agcgtaaggt gtatacattg attgaagaag gggagacctt
     9001 tggagcaagc aagctcaaca gcattatgaa ggaagttttg acggatttct ggggagatgc
     9061 tattgaaatt gacgatgatg caactctgac ttggatgcgc caagctcact actatatggg
     9121 cttgtatagt tacacttact cagcaggact agttatctcg actgctggtt accttcatct
     9181 aaaacattct gaaactggag ctgaagactg gctcaatctc ctcaaatcag gtggtagcaa
     9241 gacaccactt gagtcagcca tgattatcgg tgctgatatt tcaacagaca aaccactccg
     9301 tgataccatc caattcttgt ctgacacagt tgaccagatt atctcctata gtgctgagtt
     9361 gggagagtag gggaatagaa aaatttttta gaatgatgtt tatgggcttg ttaagcgtca
     9421 ttctatttgt cttagttgta gctattttca aaaatgtcgt tgaaggtcgt gatgttaaat
     9481 ttgaacttac tgtagaactg gtattgatag ttatttacgt cattttatat cagattgtat
     9541 ttaattgaga attcaatatt gagtcattta agactgcctt tttgaacagt tgctagaaat
     9601 gtagaggtat tgaccatgta tcaagattta ctaagaaaaa tagcagaaga aaaaccaaat
     9661 tataaccagg aagaaatcca gtggttgttt gatcatttgg gaaatccttc cccagaaatt
     9721 cgtaatgtcc ttttaaatca aggcttgcac tatctttcaa aagaaaagga tacaaggggt
     9781 ttctcaagtc agtatggttg ggttcacgct tttgcacatg gagccgattt actgacagag
     9841 gtggtttgtc atccagactt tcctaaaaac agagttcatg aagtatttga tatacttggc
     9901 caactattta aaagaatgtc gattcgcttt acagatgatg aggattggcg tttagcaaga
     9961 gtgatctatg aacctatttt acaagggaag ttggagcaag aacaagtagc ttcttggata
    10021 aaaactgttg attttccgat agaagaaagg gaggattttt acaaattttc caactttaga
    10081 tcctgtctgg tggaagtcta tgtccaactt gaccagagaa atagtttaca agatgacctg
    10141 aaagaagcta tccagtcttt tcaatactag ggattagcta atagttctat gattttcaaa
    10201 aaatttccaa tcaattttct tgaaaccctt tcctactttt gatagactag tatctagttt
    10261 ttgaaaaaag gagaaagaaa atgaaaaaat ttgttgctga gttaatcggt acgttcatgc
    10321 ttgtattcgt cgggacagga gctgttgttt ttggaaatgg tcttgatggc cttggtcacc
    10381 ttggaatcgc ctttgccttt ggtttggcaa tcgtggtggc agcctactca atcggaactg
    10441 tttcaggtgc tcacttgaac ccggctgttt cgattgctat gtttgtaaac aaacgtttgt
    10501 catcttcaga acttgtaaac tacatccttg gtcaggttgt tggagctttc atcgcttctg
    10561 gcgctgtctt cttcctcttg gctaactcag gtatgtcaac tgctagtctt ggtgaaaatg
    10621 ccttggcaaa cggtgtcact gtctttggtg gtttcttgtt tgaagtcatc gcaactttct
    10681 tgtttgtatt ggttatcatg actgtgactt cagaaagcaa gggcaatggc gcgattgctg
    10741 gtttggtaat cggtttgtca ttgatggcga tgattcttgt cggattgaag attactggac
    10801 tttcagtaaa cccagctcgt agcttggcac cagctgtctt ggtaggcggc gcagcccttc
    10861 aacaagtttg gattttcatc cttgcaccaa tcgctggtgg agttcttgca gcccttgttg
    10921 caaaaaattt ccttggaaca gaagaataat tgaaactcaa aaagccttgc tcctcatctt
    10981 gaggaacagg gctttttcgt atgatactct tcgaaaatct cttcaaacca cgtcagcttc
    11041 atcttgccgt aggtatggtt actgacttcg tcagttctat ccacaacctc aaaacagtgt
    11101 tttga
//
LOCUS       DAVKNL010000061        11054 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVKNL010000061 DAVKNL010000000
VERSION     DAVKNL010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11054
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.061"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            10..350
                     /locus_tag="VW411_001710"
                     /pseudo
     CDS             10..350
                     /locus_tag="VW411_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508814.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            885..1577
                     /locus_tag="VW411_001711"
     CDS             885..1577
                     /locus_tag="VW411_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEU9794403.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            1630..4041
                     /locus_tag="VW411_001712"
     CDS             1630..4041
                     /locus_tag="VW411_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEU9794404.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALATTSYVYKGTRFE
                     RKAFASFPDDLLVQCFTKEGLETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQLNYWPAYVTNLLETVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYLFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAARASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLQNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            4291..4992
                     /locus_tag="VW411_001713"
     CDS             4291..4992
                     /locus_tag="VW411_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9794405.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANCDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQALTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIQMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            4994..7702
                     /locus_tag="VW411_001714"
     CDS             4994..7702
                     /locus_tag="VW411_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU9794406.1"
                     /translation="MIKRKTYWKDLVQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEELEQILSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLAFVLSLLASVLPAYLVAWRELHDEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKAL
                     YKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVVAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLIAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(7798..8289)
                     /gene="tpx"
                     /locus_tag="VW411_001715"
     CDS             complement(7798..8289)
                     /gene="tpx"
                     /locus_tag="VW411_001715"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEU9794407.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(8412..9341)
                     /gene="psaA"
                     /locus_tag="VW411_001716"
     CDS             complement(8412..9341)
                     /gene="psaA"
                     /locus_tag="VW411_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEU9794408.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEANLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(9373..10221)
                     /locus_tag="VW411_001717"
     CDS             complement(9373..10221)
                     /locus_tag="VW411_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU9794409.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(10218..10940)
                     /locus_tag="VW411_001718"
     CDS             complement(10218..10940)
                     /locus_tag="VW411_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU9794410.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
BASE COUNT         3236 a         2060 c         2524 g         3234 t
ORIGIN      
        1 gtcggtcttg tgggaggaat tttacttctt attggtcctt ttgtcttgtt gggaatagcg
       61 gtaaacacag ctgctacaac tcttaatgga ggagctactg caggggcttt ttcaggtgta
      121 gccttactct tgaatgcctt gaagattgca aatcttgttc ttggtatcat tgctattgtt
      181 tactataaag gagataagcg tgtaggtgca gctccgtctg tactaatgat tgtttctggt
      241 ggagttagtc tcattctatt ccgttcttag gatgggttgg ggggattttt gctattatcg
      301 gaggatctct attcctttca acattgaaga aattcaaatc agaagaataa aaggtatttt
      361 agcatgaaaa gaacaaaaaa gtttatcggt ataggagtag ctctattatc tctttctctt
      421 ctagttgcat gtggaacata aagttcaaag aatacttcaa caagtaatga tgagaagaca
      481 gtagcaacat ccaatagttc aaaagaaaca atcactttcg atacaccggt tgtaacagac
      541 gatgcgattg aatcaatacg cacttatgca gattatatag atctttataa aaatattttt
      601 gatgattatt ttactaaagc tgaggaaggt ttcaaaggca tagctatgga aaataatgac
      661 tcgtttacta aactaaaaga gtcaactcaa aaattattcg atgcgcagaa aaaaaggtta
      721 aataatgaag atagaataga aacaaccaaa aacaatgtga ttgccaaaca ttgtcaaaca
      781 gtcctttcct ttttggtttt gactagcttt tttgtgaaaa attgtgtaaa atagaataga
      841 taaacgaggg gaaacctcgg aaaatttaaa ggagaatcca tctaatggta aaattggttt
      901 ttgctcgcca cggtgagtct gaatggaaca aagctaacct tttcactggt tgggctgatg
      961 ttgatttgtc tgaaaaaggt acacaacaag cgattgacgc tggtaaattg atcaaagaag
     1021 ctggtatcga atttgaccaa gcttacactt cagtattgaa acgtgctatc aaaacaacta
     1081 acttggctct tgaagcttct gaccaattgt gggttccagt tgaaaaatca tggcgcttga
     1141 acgaacgtca ctacggtggt ttgactggta aaaacaaagc tgaagctgct gaacaatttg
     1201 gtgatgagca agttcacatc tggcgtcgtt catacgatgt attgcctcca aacatggacc
     1261 gtgatgatga gcactcagct cacacagacc gtcgttacgc ttcacttgac gactcagtta
     1321 tcccagatgc tgaaaacttg aaagtgactt tggaacgtgc tcttccattc tgggaagata
     1381 aaatcgctcc agctcttaaa gatggtaaaa acgtattcgt aggagctcac ggtaactcaa
     1441 tccgtgccct tgtaaaacac atcaaaggtt tgtcagatga cgagatcatg gacgtggaaa
     1501 tccctaactt cccaccattg gtattcgaat tcgacgaaaa attgaacgtc gtttctgaat
     1561 actaccttgg aaaataaaaa attgtaagtc tagaattgat ttctaggctt tttatgttag
     1621 tatggaagta tgataaggaa taaaaaacaa gattatgtac tggcctacaa gcaaccagct
     1681 tcaaccactt acatgggttg ggaagaagaa gctttaccga taggcaatgg ttctttagga
     1741 gcaaaagtat ttggccttat aggggctgaa cggattcaat ttaatgaaaa aagtctctgg
     1801 tctggaggtc cacttcctga tagttcagat tatcagggtg gaaatcttca ggatcagtat
     1861 gtttttttag ctgagattcg gcaggctttg gagaagagag attacaatct ggctaaggaa
     1921 ctggctgagc agcacctaat tgggccaaaa acgagtcaat atgggaccta tctgtctttt
     1981 ggggatattc acattgagtt cagccagcaa ggtacgactt tgtctcaggt gacggactat
     2041 cagagacagc tgaatattag taaggcactt gcgacgactt cttatgtcta taagggaacg
     2101 cgatttgaac gtaaagcttt tgcgagtttt ccagatgatc tcttggttca atgttttact
     2161 aaggaagggt tggaaactct agattttact atagaactat ccttgacctg tgatttggct
     2221 tctgatggaa agtatgagca ggaaaaatct gattacaagg agtgtaagtt ggatattact
     2281 gattctcata tcttgatgaa gggaagagtt aaggataatg atctgcggtt tgctagttat
     2341 ctagcttggg aaacggatgg agatattaga gtttggtcag atagggttca gatatcagga
     2401 gccagttatg ccaatctctt cttggccgct aagacggatt ttgcccaaaa tcctgctagc
     2461 aattatcgca agaaactaga tttagagcaa caggtgatag acttggtgga cacagctaaa
     2521 gaaaagggct atacccaatt gaaatcaagg catatcgagg actaccaagc cttattccag
     2581 cgtgttcaat tggatttgga agctgatgtt gacgcatcca ctacagatga tttgttaaaa
     2641 aattataagc cacaagaagg gcaggctttg gaggagctgt tcttccagta tggacggtat
     2701 ttattgatta gttcgtccag agactgccca gatgctctac cagctaacct acagggagtc
     2761 tggaatgcgg tcgacaatcc tccttggaat tcggactatc acttaaatgt caatctgcag
     2821 ctgaattatt ggccagccta tgttaccaat ctcctagaga cggtctttcc agtcatcaac
     2881 tatgtagatg atttgcgtgt ctatggtcgt ctagcggctg taaagtatgc aggaatcgtc
     2941 tctcagaaag gtgaggagaa tggttggttg gttcatactc aagcgactcc ctttggttgg
     3001 acggcacctg gttgggatta ctattggggt tggtcaccag ctgccaatgc gtggatgatg
     3061 caaaccgttt atgaagccta tttattttat agggaccaag actatctcag ggagaaaatt
     3121 tatcccatgt tgagggaaac ggttcgtttt tggaatgcct ttttacataa ggatcagcag
     3181 gcgcagcgtt gggtgtcttc tccgtcttat tccccagaac atgggccgat ttcgattggc
     3241 aatacctatg accaatctct gatttggcag ttatttcatg attttattca ggctgctcag
     3301 gaattgggac tggatgagga cttgttgact gaggttaagg agaagtctga tttactaaat
     3361 cctttgcaaa tcactcaatc tggtcgaatc agggagtggt atgaggagga agagcagtat
     3421 tttcaaaatg agaaagtgga ggcccagcat cggcacgctt cccatctagt gggactctat
     3481 cctggcaatc tctttagcta caagggacaa gagtatattg aagcggcgcg tgctagcctc
     3541 aatgatcgtg gagatggcgg cacaggctgg tccaaggcta ataagatcaa tctctgggcg
     3601 cgtttgggag atggcaatcg agcccataaa ttattggcag agcagttaaa gacatccacc
     3661 ttgcaaaatc tttggtgtag ccatcctcct tttcagatag atggtaattt tggtgctact
     3721 agtggcatgg cagaaatgtt actccagtct catgcagctt atctggtacc tctagctgcc
     3781 ctacctgatg cttggtcaac aggttctgtt tcaggcttaa tggcacgtgg acattttgaa
     3841 gtgagcatga gctgggaaga taaaaaactc ttacagttga ccattttatc aaggagtgga
     3901 ggagatttgc gagtttctta tccagatatt gagaagagtg tgattaaaat gaatcaagaa
     3961 aaaataaaag cgaaatgcat ggggaaagat tgtatttcgg tggcaacagc agaaggtgat
     4021 cttgttcaat tttattttta agaagatgtt ataaggcagt aatttgaaac tgccttttaa
     4081 taaggattta agaatataag cagttttcaa ctagttgaaa aaacgttata atgataatag
     4141 gaagtaatac tcaatgaaaa tcaaagagca caaactagga agctagccgc aggttgctca
     4201 aaacagtgtt ttgaggttgc agatggaagc tgacgtggtt tgaagagaga ttttcgagga
     4261 gtataatttg tttgatagag ggtgggtctg atggcttata ttgagatgaa acactgttac
     4321 aagcgttatc aggttgggga cacggagatt gtggccaatt gtgatgtgaa ttttgagatt
     4381 gaaaaggggg agctggttat tatccttggt gcttcaggtg caggcaagtc aacagttctt
     4441 aaccttcttg ggggaatgga taccaatgat gaaggggaaa tctggattga tggtgttaat
     4501 attgcggatt atagttccca ccagcgcacc aattaccgta gaaatgatgt ggggtttgtt
     4561 tttcagtttt ataatctagt ttctaatctg acagctaagg aaaatgtgga actggcttct
     4621 gaaattgtga cagatgcctt gaatcctgat caggccttga cagatgtagg tctggctcat
     4681 cgtctcaata actttccagc ccagctttct ggaggggagc aacagcgagt ctccattgca
     4741 cgcgcggtag ccaaaaatcc taaaattctc ctttgtgatg aaccgactgg agccttggat
     4801 tatcagacgg gcaagcaggt tttgaaaatt ctccaagaca tgtctcgtca aaagggagcg
     4861 acggtgatca tcgtgactca taatggagct ttggcgccca ttgctgatcg cgtgattcaa
     4921 atgcacgatg ccagtgtcaa ggatgtggtg ctcaaccagc atcctcagga tattgacagt
     4981 ttggagtact agcatgatca agcgaaaaac ttattggaag gacttagttc agtccttcac
     5041 aggctccaag gggcgttttt tatccatctt gatcctgatg atgttgggat ctctagcctt
     5101 agtaggcctc aaagtaacca gtcccaacat ggaggcgaca gctaatgctt atttaacaac
     5161 tgctcaaacc ttggatttgg cagtcatgtc taactatggc ttggatcaag cagaccaaga
     5221 agaactaaaa cagacggagg gcgcagaggt cgagtttggc tatttgacag atgtgactat
     5281 ggataatggg caggatgcca ttcggctgta ctccaaacca gagcgaattt caacctttca
     5341 gctaagaaag ggacgacttc ctcagtcaga caaggaaatc gctttggcca ctcatttgca
     5401 aggccaatac agcgtgggac aggagattag ttttaaagaa aaagaagagg gtcattcctc
     5461 tttaaaagac catacttata ccattactgg ttttgtggat tcggctgaaa tcctctccca
     5521 gcgagatatg ggctacgcag gaagtggaag tgggactctg acagcctatg gggtgatttt
     5581 acctagtcaa tttgatcaga aagtctacaa tatagctcgt ttgaaatatc aagatttagc
     5641 gggtttaaat gccttttcat cagcttatga agaaaaatcc aagcaacatc aagaagagct
     5701 tgaacaaatt ttatcagata atggcaaggt acgtctgcaa cttttgaaaa aagaaggaca
     5761 agagtctcta gacaaggggc aagagaccct tgacaaggct cagactaatt tgcaggaagg
     5821 caagcgtcgt ttagcagctg ctcaagctcg tatacaggct caagaaagtc aactagcctt
     5881 gtttcctcaa gttcagagag agcaggctag tgctcaactt acccaagcca agcaggaatt
     5941 gggcaaggaa gaggacaaac taaagcaagc tgaacaaaat ctagcccaag aaaaggaaaa
     6001 attagaaaaa catcagcaag tcttggatga tttggcggag ccaaggtatc aggtttataa
     6061 tcgtcagacc atgccaggtg gtcagggcta tcttatgtat agcaatgctt catccagtat
     6121 tcgagcagtg ggcaatatct ttcctgtggt actttatgcc gtagcagcca tggtgacctt
     6181 tacgaccatg actcgctttg tagacgaaga gcgaactcat gcagggattt ttaaggcctt
     6241 gggttatcgt agtaaggata ttatcgccaa gtttctcctt tatggactag tagctgggac
     6301 tgtcggaacg gctctaggta gtatacttgg tcattatttg ctagccagtg taatttcaag
     6361 tgtcattaca aaaggcatgg tggtgggaga aactcagatt cagttctatt ggacctatag
     6421 cttactagct tttgtcttga gcttgttggc gagtgtgtta ccagcctatc tggtggcttg
     6481 gagggaactt catgacgaag cagcccagct tctacttcct aaacctcctg tcaaaggagc
     6541 taaaatctta ttggagcgta tcggttttat ctggcgtcgt ctcagtttta ctcataaggt
     6601 aacagcccgc aacatctttc gttataagca gagaatgttg atgacaatct ttggtgtggc
     6661 aggttctgta gctctgctct ttgcaggttt gggaatccaa tcttctgtag caggagttcc
     6721 gtctaaacag tttcaacaaa tccaacagta tcagatgctt gtctctgaaa atcctagtgc
     6781 gaccaatcag gacaaggtag agctagcaga agtgttgaaa gggcaggaga tactagccta
     6841 ccagaaaatc tattctaaag cgctatacaa ggatttcaaa ggcaaagctg gtcttcaaaa
     6901 cattactctt atgatgatag agaaggaaga tttgactccc tttatccatc ttcaacatca
     6961 tcagcaggag ctgacattaa aagatggcat cgttattaca gctaaactcg cccagctggc
     7021 aggtgtcaag gttgggcaga ctttagaaat tgaaggtaag gaactaaagg tcgttgctat
     7081 tactgagaac tacgttggtc actttattta tatgagtcag gctagctatg agcaacttta
     7141 cggacagcta ccccaagcca acacttatct ggtctcatta agggatacca gtgcaactag
     7201 tatcgaaagt caggcgggct tgcttatgaa tcaatctgcg gtgtccagcg ttgtccaaaa
     7261 tgcttcagcc attcgactct tcgactctat cgctagctca ctcaatcaga ccatgaccat
     7321 cttggtcatc gtatcggttc tattagctat tgtcatcctt tacaatctga ccaatatcaa
     7381 cgtagctgag agaatccgtg aactctccac tatcaaggtt cttggttttc ataataatga
     7441 agtcaccctc tacatttacc gtgagacgat tgtgctgtcc cttgtgggaa tcgtacttgg
     7501 tctgatagct ggtttctatt tacaccaatt tttgattcaa atgatttcgc ctgcgactat
     7561 tctcttttat ccgcaggtag gctgggaagt ctatgtaatc ccagtggcag cagtaagcat
     7621 cattttgacc ttgcttggtt tcttcgtcaa ttattatctg agaaaggttg atatgttaga
     7681 agccctgaaa tctgtagagt aaggtagtta tttttagctg attgaacttc tatttactaa
     7741 tattcaaaaa tcctccgttt caaagagcag ggaactcttt gtgacagagg attttttcta
     7801 tagggcttta gcagctgcaa ttgcggcttc gaagtttggc tcagaattga tattatccac
     7861 gtattcaacg tagcgaatcg tattgtcagt atcgaggaca aagactgcgc gtgctaatag
     7921 gtgccattcg ttgatcaaga gggcataatc gcgcccgaaa gaatggtcaa agtagtctga
     7981 aagcataatg gcattgtcaa ggccttcagc accgcaccaa cgtttttgag caaaaggtag
     8041 gtccattgaa acagtcaata cgaccgtgtt gtccagtcca gccaattctt cattaaaacg
     8101 acgtgtttga gttgagcaga tgcctgtatc gatagaagga acgacactca agactttttt
     8161 cttgccatca aaatcagcca gagatttttt agaaagatct gttgtagtaa gagaaaaatc
     8221 aagcgccttg tcgccgactt gtagttgttt acctgtaaag ctcacaggat ttccgagaaa
     8281 agttaccata ggatactcca atcttttttc ttccatttta gctgaaacag tcggaatttt
     8341 ccaatgattt gaccggaaat atgggcatag aaaaaacgcc agctcatgtg agaatgacgt
     8401 ttttcatagg tttattttgc caatccttca gcaatcttgt caaggttgta tttcatcatg
     8461 ctgtagtagc tgtcgccttc tttaccttgt tctgcgatag agtcagtaaa gatttgagcg
     8521 tagattggga tgtttgtgtc ttgagaaaca gttttcattg gacggtcatc cacacttgat
     8581 tctacaaaga gtgatggaac ttttgtttgg cgaagttttt caaccaaggt cttgatttgt
     8641 tcaggagttc cttcttcttc agtattgatt tcccagatgt aagcacttgg gacaccatag
     8701 gctttagaga agtatttgaa tgctccttcg ctggttacaa tgagtttctt ttcagcaggg
     8761 atcttattaa atttatcctt actttcttta tcaagtttgt ctaacttatc agtatattct
     8821 ttgagatttt tttcatagaa ttctttattg ttagggtctt tggcgctcaa ttgtttggcg
     8881 atatttttag caaaaataat accgttttca aggttaagcc aagcgtgtgg gtcttctttt
     8941 cctttttcat tttgaccttc aaggtagata acatcaacgc cgtcgctgac tgcgaagtag
     9001 tctttgtttt cagttttctt ggcattttct accaattttg taaaccaagc attgccacct
     9061 gtttcaaggt tgataccgtt atagaaaatc aaattagcct cagaagtttt cttaacgtct
     9121 tcaggaagtg gttcgtattc gtgtgggtct tgcccaatcg gaacgatact atgaaggtca
     9181 attttgtcac cagcaatatt tttagtaata tcagcgatga ttgagtttgt agcaacaact
     9241 tttagttttt gaccagaagt tgtatctttt tttccgctag cacatgctac aagaatgatt
     9301 gcagaaagaa agagaacgag taatgtacct aattttttca ttagatcctc caatttatta
     9361 gggctttgcc ccttatttta acaaatgttt atttttcagt ttcaaatatc gttgtttggg
     9421 agcgataaag aagctaatga gaaagaaact agcagctgta agcacgatac tagaacctgc
     9481 cgcaacatta aaactatagc caataaagag tcccaaaact gaagcagtag ctccgaaggt
     9541 tgaggaaagg aaaatcatac ttttcagact attagcatac agataagcag ttgcagctgg
     9601 ggtaatcagc atggctacaa tcaggatagt tccgacactt tgcatggctg tcacagacac
     9661 gagagtcagg agtaccatga gaaggtagtg atagaaattg acaggcattc ccatggcttt
     9721 agccaagagt tcatcaaagg aagttatcaa gagttgcttg aagaaaatcc agattaacaa
     9781 gaggatagct gcccccacac ccatagtaat aaacatatcc gtatcttgga cggccaggat
     9841 attaccaaaa aggatatgga aaaggtcagt tgaactttta gcgacaccaa tcaagatgat
     9901 accgagggct aagaaagaag aaaaggtaat gccgatggcg gtatcgcttt tgataatcga
     9961 gtttcctttg atgtaggtaa tgatgatggc agctagcaat ccaaagacaa tggctccgat
    10021 aaagaagtca aggcccaaga tgaaggatag ggctacacct ggtaagacag catgtgaaat
    10081 ggcatctccc atgagtgaca tcccgcgtag aataatgaaa catcccacag ctccagctac
    10141 aatcccgacg acaatagctg ttatcaaggc attttgtagg aaatggaatt tttgcaatcc
    10201 atcgataaat tctgcaatca taggtcacct ccattgaaaa agagttgatt accgtaagct
    10261 tcttttagat tggtttcggt aaaagtttct tttgttggac caaaggcaat cacttctcga
    10321 ttgacaagta agacttgatc gaagtagtgg ggaatcttgc tgaggtcgtg gtgaacgatg
    10381 agaaccgtct tcccagcttt tttcaaatct ctcagcgtat tcatgatgat ttcctcactg
    10441 acagagtcaa tcccagcaaa gggttcatcc aagaggatat agtcggcttc ctgcaccaaa
    10501 catctggcaa tcaagacccg ctggaattga cctccagaca gttgactaat ttgacgttca
    10561 gcgtagtcag ctaggccgac gatttcaagg gcctcttgca ctttcttcca atgtttagcc
    10621 tttaaacttc gaaagagagg aatagaggga aatagtccta acgagacgca ttccttgacc
    10681 ttgatgggaa agttgtagtc gatattgatt ttttgttcga cataggcaat tcggtgtaag
    10741 gattttttaa cttccttgtc atcgagaaat gcctgacctt gatgtgggat aattcccaac
    10801 atacctttta atagtgttga tttcccagcg ccgtttggac caatgatgcc ggtaattgtt
    10861 ggtccatgga gcactagtga aatatcctta agtgccaacg tttctttgta ggagacactg
    10921 aggttttcga tacgtatcat aaacttgtat tcctcctgtc tcttaatata cattaaaaaa
    10981 aaaattaagt caagttaatt tttgaaaaaa ttaaaataat aactgaaaaa tagattctaa
    11041 agataacttt cagg
//
LOCUS       DAVKNL010000062        10762 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVKNL010000062 DAVKNL010000000
VERSION     DAVKNL010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10762
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.062"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(123..1547)
                     /locus_tag="VW411_001719"
     CDS             complement(123..1547)
                     /locus_tag="VW411_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU9794411.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAVERAAYLHKTAAILERDKEEIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEATSKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2092..4371)
                     /gene="pulA"
                     /locus_tag="VW411_001720"
     CDS             complement(2092..4371)
                     /gene="pulA"
                     /locus_tag="VW411_001720"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEU9794412.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGYLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4483..6441)
                     /gene="ligA"
                     /locus_tag="VW411_001721"
     CDS             complement(4483..6441)
                     /gene="ligA"
                     /locus_tag="VW411_001721"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU9794413.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSCEELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQARQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KVSQLLLQYFHSIENLSQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSISKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6534..7712)
                     /locus_tag="VW411_001722"
     CDS             complement(6534..7712)
                     /locus_tag="VW411_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9794414.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLYSFLIIGLGVVALYFLALGYVRTHFEKLI
                     "
     gene            complement(7792..8655)
                     /locus_tag="VW411_001723"
     CDS             complement(7792..8655)
                     /locus_tag="VW411_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU9794415.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTPLYFELNWILLQGLICQRDASYDMKQDDLGKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8658..10559)
                     /locus_tag="VW411_001724"
     CDS             complement(8658..10559)
                     /locus_tag="VW411_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9794416.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNDLRRTERQLR
                     QMELEMGEKSGEYLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETIAWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLVTEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKSANMTFQSEKTSGNVVLTVENAAV
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSEQV"
BASE COUNT         3227 a         2371 c         1916 g         3248 t
ORIGIN      
        1 tagaggtgta ctattctggt ttcaatctat tataatacca aaaagttcgt attttttcta
       61 taattattat tattttatag caaaaaaata aaaccaggaa aacaatttcc tggttttaca
      121 cgttatttca catcaaaaac aatggatttg acatttgtca tcgcttcaat gctatattta
      181 attccttgca ctccagctcc agaacctttg acaccaagga atgggaaatt atctggacca
      241 cgctgggttt tattattaat gtggactgta cctacttcaa gtttttcagc aatttcaaag
      301 gcttttttga aatcatttgt aaagactgat gattgaaggc cgaattcaga ttcgttggca
      361 aaggcaatag cttcctctac actagccaca cgaatgattg gtaaaacagg accaaatggc
      421 tcttcccatg ccactttcat atcttttgta acttggtcaa aaagcactgg ccagagaaga
      481 ttgccctcac gtttgattgg tgtaagagcc tgagcttctt tttcttgtgc atcctcaatc
      541 aagccccaaa tgaagtcggc tgaagcattg tcaataacag gtgtaatatc agcattgtca
      601 aatggatcac caactgttaa tttagaaact tcttcctgaa gcaaagtagc taatttatct
      661 gctacacttt cgagaacaat gacacgttta atggccgtgc aacgttgtcc tgagtagcta
      721 aaggctcccg caacaatttg cttggcagca tgttccaaat ctgcatcttc tagtacaaga
      781 gctgcatctt tcccaccaag ttccaacatg ataggacgca taccagctaa acgaccaata
      841 cgttctccaa taggagttga acctgtaaag ttgatgaagt tgacttcttt gtgctcaatg
      901 atataatccc caatttctga accacgacct gtaatggtgt tgaaaacacc tgccggaatc
      961 cctgcttctt caaatgcttt agccaacaag agtccagaaa tggaaccttg tgttggtggc
     1021 ttaaacatga ccacattccc tgcaatcaag gcaggtgcaa ttttagaagc agataaatta
     1081 actggataat taaagggagc aatcgctagc acgataccaa ctggttcacg acggacaaca
     1141 gccagtttgt ttttacttgt tgcctcaaaa ccaccacctt ccattgcttg tccagtgata
     1201 cggagacctt cctcagcagc ataacgaatc aagtctgctg tacgcactac ttctccaatt
     1261 gctgctttaa tcccttttgc tacttctttg gcaaggatag taccaatttc ttccttatcg
     1321 cgttctaaaa tagctgctgt tttatgcaaa taagccgcac gttcaactgc tgataaagct
     1381 cgccatgctg gcagggctgc acgcgcagct tgcatagcct catcagcttc agtctgagtc
     1441 atggctggaa ctgtacccaa ttcttcttga ttgattggtg aataaatcgt aatttcttgt
     1501 tcagatgatt tccattttcc atttactaaa ttctgatatc ttgtcaaatt tttgtcctcc
     1561 tgaaaatgat taagatatat tttaccaaat tttcagaaaa tcacaaccct ttttgcaaaa
     1621 tttttgagat tattttcaca aacttgattt ttcaaagtat actcaataaa aattaaaaaa
     1681 atccactacg tcaaggcgag gctaatgtgg tttgaagaaa ttttcgaaga gcgtgaatga
     1741 gtatcatcta tagtaaaata aaaaaactga acaatttggt tggggacagc caaaccaatt
     1801 tctcacaatg tttcagaaac aagggtgtgc tattccaatt tcagcctact ataactgtca
     1861 tagattgctg aaacaaagtc taggtaaaag tcttcataat aaaaagacct cctatcaagt
     1921 gttcaaaaac tttgatagga ggtcttgttt tgtgaaaata tttatcaaat tttctataca
     1981 agtgagctgt tagccaggtt ctttctattc tttcaatttc aatgaatgga ttttttacta
     2041 atactcataa ctgggaattt gtctgtgtaa aaatagcgag atagatggta tttataaaac
     2101 actcaagaca gctagactaa tatcatttaa aacattatct tcttttgagc gactgttggt
     2161 taccaacata gctaaatttc ctgcattttc aaattgatag ggttctgatt tagcattcac
     2221 aaccaccaag aggtgttctt tgccgtgaac ttcatagata aggtagccgc tatgttcaat
     2281 cgcagaatgc acaaagacat gatggtaaat ttcatcatag ctagagtaag aaaaggcacc
     2341 agtttttgtc ttcaatcgga tgacttgacg gataaactca atactgtctt gacgctcatt
     2401 aatcaagttc cagttcactt ggttcacact gtcaggagca ttatagctat tcatcgcacg
     2461 ctctctatca tcatgggtca actcaccatt ttcaccagtc gcaaccagtt tggtacgacc
     2521 aaattcttga ccgatttcca taaaggccat cccctgcatg agcagattca tggctgtggc
     2581 agtttcgacc ttgcgcatga tttgctctga actttggtct ggatgaaggg ttgccaataa
     2641 atcgtgaaga ttgtaattgt catgggcttc tacatagtta agcacctgat ttggatgtgt
     2701 atagcttcct aattcacgac ttcctaggat tgctttagct agaattggct ctgtcgcagc
     2761 accactgaca aaacctgact tgatagcacc ataaacttct ccccctttga cagcatcgcg
     2821 ctgattgtca ttaaagaaac caatatttgg catctggtag gcattgtcct tcttggcctt
     2881 atcataaggg gcaagacctg ttcccatatc ccatccttct ccatagagga taatgttgga
     2941 gtcgatttca tccaagcttt gacgaatcat ctgcatggtc ttgacatcat gaatccccat
     3001 caagtcaaaa cggaagccgt caatattata ttcctgcacc cagtatagaa gagaatcaat
     3061 catatacttg cgaaacattt cgtgttcact ggctgtttca tttccaacac ccgttccatt
     3121 ctggaaggta ccatctggat tcatacgata atagtaatca gggactgttg tttggaatgg
     3181 tgcatcaaca actgagaagg tatggttata gactacatcc ataatgactc caatacccgc
     3241 atcgtgataa gcttgaacca tcaccttcaa atcacgaatg acctgagctg gatcatctgg
     3301 attagttgaa aaactagttt ctggcgcgtt atagttttgt ggatcataac cccagttgta
     3361 ggttacattt ccatcctcat cgtattcttt atgacggtct gcaattggtt gcaattgaac
     3421 ataattgtag cccagcttct tgatgtaatc aaaagcagtt gactggccgt attggttaac
     3481 tgttccagcc tgagcagcac ccaagaaagt tcctcgaaga tgttcatcta cacccgatgt
     3541 aggtgattta gtcaaatcac gaatgtgcat ttcacagata actgccttac atggattttc
     3601 caagcgccaa gtagcctccg aaccgtgctt aacctcgaag ttttcaactt gcttttctac
     3661 atggctcaga atagctgaac gtttgccatc agggctggtc gcgattgtat aaggatcacg
     3721 tgtcagtgtt tggtgatgag ggaattggac ttgatactga taagtcttac ctaccaaatc
     3781 ttcttcaaca tccaaactcc agacaccgat tgtattgtcc ttatgattat aagagtagct
     3841 attgcctctt ttcatctcaa aagtcttcca aacgggtgca tcattagcag ctgattcata
     3901 aacgacaact tgcacttctg tcgctgtagg tgaccagaga gaaaaatgag cctgattgtc
     3961 ctctacacgg caacccaatt ctccttggta accccaatga tgatcaaaac tagcactgtt
     4021 aatggcctta tcaaaggcaa aaggattttg atttttatag aaaggactgg caatagcagg
     4081 attttcagag taataaatcc tatcatcgcc ttccaaaatc cagacctctg ttaatagggg
     4141 atagtgatta aaacggatag aatattcttt actagtttga cctgtatgaa ccacaaaatt
     4201 caagctttct ataacatgtg aacttgggtg ttcaaagcta aataaagctc caaaataatc
     4261 ttctttgtag gttagcaaat caattcgttg atcctgactt tttacaaagg agcaagtgtc
     4321 atattctcca ttcttacgat ggtaatgaat gcgcataggg tagttataca ttttttattt
     4381 ttccttttta ctttgtttct atttcactaa taaatttttg tcaatctcgt ctcaattaac
     4441 agacatagtc atattctcta aactctgttt ttaaacgatc cattacaaac tttctagcca
     4501 tgcctcatct ctgacctgga taccaagttc ttgtgctttt tgcagtttac ttccagcgtc
     4561 tgcacctacc acgacgaggt cggtcttttt agaaatacta cctgtcactt tggcacccag
     4621 actttcgagt ttacttttag cttctgagcg cttgagtcgt tccaattttc ctgtcaatac
     4681 cacggtcaaa cctgacaagg ccgcatccgc tactaccgtc tgtcctttat agtccagatt
     4741 gaccccagtt tctttcaatt ctctgagcag aatttcagag ccttctgtcg caaaataagt
     4801 ctgaagactt ttggcaatca cgccacctag actttcaata ctagccactt cctctgaatc
     4861 tgcctgagac agattttcaa ttgaatggaa atattgaagt aaaagctgac taaccttgct
     4921 tccgacatga cgaattccca aaccaaataa gagcttctcg gcagaatttt cctttgatgc
     4981 ttggatagcc tgatacagtt tagcagcgga cttttcctta actccctcta aaaggaggaa
     5041 atcctcttct tgcaaacgat aaatatccgc cacatccttg actaaattag cagcaaaaag
     5101 cttctcaaca atagatggac caaggcctgt aatattcata gcatcacgag aagcaaagtg
     5161 aatcaagcct tccatgattt gagcagggca acgcggattg atacaacgta gggccacttc
     5221 atcttcaaag tgcaacaagt cagagttaca acttggacag tttgtaggga tatctagttt
     5281 ttcttcagaa acccgtttgg actctaccac acgtaaaacg gcagggatga tgtcaccagc
     5341 cttatataca atgaccgtat cgtcttttcg gatatctttt tcagcaatat aatctacatt
     5401 gtgcagggtc gcacggctaa cagtcgtacc ggcaagttgt actggtgtta gattagcagt
     5461 tggagttaca acaccggtac ggccaactgt ccagtcaact gataagagtt gagcttcttt
     5521 ttcttcggca gggaacttgt aggctactgc ccactttgga gccttaactg taaaaccaag
     5581 ttcttcttga cttgctaggt cgttgacctt gattaccact ccatcaatat cgtaaggcag
     5641 attttcccgt tcctgtccta cttcttggat aaaattccag atttcatcta tgttttcagc
     5701 caagattcgc ttaggattga ccacaaaacc tagttgttct aggtacttca aacccttttc
     5761 ttggctatca cgagttgaag ggctggcttc ttgatagaga aacgttgcaa gattacgctt
     5821 ggcaactact gctgtatcca actgacgcag agttcctgct gccgcattac gaggattagc
     5881 aaattcaggc tctccatttt cttggcgcgc ttggttaact tggtcaaagg aagcgcgtgg
     5941 catgtaacat tccccacgaa ctgtgatatc tagttcttct ggcaaagtca aagggatgtc
     6001 cttaacacgc ttgaggtttt ctgtgatatt ttcaccaatt gaaccatctc cacgtgttac
     6061 cccagcaacc aaaatcccct tttcataagt cagcgagata gataagccat cgattttcag
     6121 ctcacaaata taggtcggat gagccacttc cttacgaaca cgcgcatcaa aagcatctag
     6181 ctcctcacat gaaaaagcat cctgcaaact ataaagagga tactgatgac tgtatttttc
     6241 aaaaccatct aaaaccttgc caccaacacg atgagtcgga ctgtctgcta gcacttgctc
     6301 tggataagca gtttctaact cgaccaactc acggtaaagg cggtcatact cactgtctga
     6361 aaccgaggga ttatcgctgg tatagtactc agtcgcatag cgattgagca aagcgactaa
     6421 ctcattcatt cttttattca taagaccatt ttaccataaa acaagccctc ctcacaaacg
     6481 agaagggcgg aaaaaacact tagtttgaaa ttatttttga aactcaagca accttatatc
     6541 aatttttcaa aatgagttcg aacatatccg agagctaaga aatataaggc tacaactcca
     6601 agtccaataa tcaagaaaga ataaagatgg acacttggca agactgtcat aaatcctttt
     6661 gcaataggca taaatagaat agctaaggta aaaattgtac tcagtactct tccaagaaat
     6721 tcgctctcaa ccttggtttg tacttgagta aaaaagtgaa tattaaaaat cgtcataaac
     6781 aattcacaaa ctaaatttcc agaaaaggaa agaaaagttg gaagtggtaa tcccatcata
     6841 aaaactccga cacctgtcaa agccagtaaa atcaaaagat tataaatatt agctttaatt
     6901 ttactagcta gaagagcccc aatgatggaa ccaatagccc ccatagttaa aatacttgca
     6961 taggctcctt ctgacccgta aagctgattc gaaaagggaa gtagaaattc aaaagctgca
     7021 aaaaagaaat taacgctgga agctaccagc aaaaggaaga aaatttcttg ctgatgccag
     7081 atatagtgta acccatcctt gatatctaca aaaatatctc tcccagtaaa agcctttttc
     7141 tcttgaactt ttgcttcctc ttttggaagg aaagccacta gaacaaaagc aatgaaaaaa
     7201 gtcagcgagt ctagcagtag cgtcatatgg agacttgcaa actgtaaaac aaggaaggaa
     7261 agaacaggag agctaacacc tacaacctgc aaaaccagct ctaagcgaga attatagatc
     7321 acaatctcat ctttctccac cacttcagtt atgatagctt tattggctgt gcgagaaaag
     7381 gcaaaagcaa tagcctgcac aatgttagca acaatcaaag cgccaatcat ccagctatca
     7441 ttccttatga aagaaatagc cagacaaaga atcccacaaa caagatctgc cgtcattaaa
     7501 atcttacgac gagaaaaacg gtctgaaata actccgccaa agggattgac gagaatagat
     7561 gtgacgagct cagaaatctg atacattcct aaaactgtct gtcctatagt ccccatagaa
     7621 gccaaccaga cactatttcc ataatcatag agcatatttc ccattttatt gatagcccca
     7681 cggctaatca actgcactgc atagcgattc atattaaagc tcctctcaaa ttttgaaact
     7741 attgtatcaa aaccgaaagg agctttttat tttttccctt atttgggaaa attaactttt
     7801 gacaaatttt tcgtagtgtt cctgataata ggctacttgc tctggaagac ctaacacatc
     7861 aaaaatatgc atggcctctt gcatctgctt acagccttct ttacactgtc ctttttgata
     7921 taaggcaaaa ccttttaaat aatggaaaac attacgctca taaagcttaa tacctttgtc
     7981 aataatcttc tctgtataag cctcaaaata gttggcatta taaaaagaag aatgctctaa
     8041 acaatgctgg taacaattga gggccaaaat caacactaat ctcttatggc gactaatctc
     8101 ttggtaaaat tcctccctct ccataacttc tctaccaatc cgagtgacat agtctacatc
     8161 gtagaaacta tagaggttac cgaaaagaat caactcatac atggtccatt cttctgtttt
     8221 gaagagataa tctgctacct tacccaaatc atcctgcttc atatcataac tcgcatctct
     8281 ttgacaaatc agaccttgta gcaaaatcca gttcagctca aaataaaggg gagtcgtcga
     8341 actcttagac ttttcaagtt gttctctttg aagcttttga aaacctgcaa tatcgtttga
     8401 atagtaaagt gggataatct gtgccatcat agacacatgt tcatgattat gaaaattcct
     8461 tgccttatcc atgaaatttt cgattgttac atgaatgtta tccaaaatct caaagaaacg
     8521 ggagactgcc aggtcagact ccccaagctc aaagcgagat aactgagagg tagagcagga
     8581 ttcgcctgct gcttccttta aagaataatt tccacttgtt cgaaattcac gaaatacttt
     8641 tccaagatgt tccatcttta cacctgctct gataattctt cccactcaag catagcttct
     8701 tcctgacgat ggctgatttt gtccagctca gcctgtaatt ccatgagttt gtcggcatcg
     8761 tttgtttcca acatttgttc agaaatggct tggctttgac tttctagctc ttcaatttca
     8821 gcttctagac tttcgatttg tcgcatgagt ttgcgaactt ctttttgact ttctttctgg
     8881 gcctgatagt cattgactgg acttgcttcc tttgcttgat tgctagttga agcttcctca
     8941 gtctgactca tttctgctgt tgctttcttc tcaacatagt agtcgtaatc tccaaggtag
     9001 agagttgaac cattctcaga caattccaaa acatgagttg ccacacgatt gataaagtaa
     9061 cgatcatgac tgacaaacag caaggttcca tcaaagtcaa tcaaggcatt ttctagcact
     9121 tccttactat caatatccaa gtggttggtc ggctcatcca gaatcaaaaa gttattgttt
     9181 tccatagaca atttagctaa aagcaaacga gctttttcgc caccagatag catgccgact
     9241 gattttttaa catcatctcc tgagaaaagg aaggctccaa gacggttgcg gatttcaact
     9301 tctggtgtca gtttgaaatc attccagagt tcatccagca ccgtattact tggtgtcagc
     9361 ttgctttggg tttggtcata gtaaccaacc tcaacattag cgccaaagcg cttttctccc
     9421 ttgataaaag gaatctggtc cacaatagac ttgataaagg ttgacttgcc gataccattt
     9481 ggaccaacga tagcgacagc attcatctta cgaagatcta ggttaatcgg ttgtgacaag
     9541 acttccccgt catagccaac agctgcattt tcaacagtca aaacaacatt gcccgacgtt
     9601 ttttcagact ggaaggtcat gttggctgat ttcttgccag cttcaggctt gtccaaacgt
     9661 tccatttttt ccagttgttt acggcgagat tgagcacgtt tagtcgttga agcacgaact
     9721 agattgcgat tgacaaagtc ttccagagca gcgatttcct tctgttgctt ttcatagttt
     9781 tttgcctcag taactagctt ttgctccttc aattcgacaa aacgagagta attccccaca
     9841 tagcgatcca aggaatgctt ggtcaaatct agcgtaattg tcgcaacctt gtccaagaaa
     9901 taacggtcgt ggctgacgat aatgagggca ccgctatagt ttaccaagta attctctagc
     9961 caggcgatgg tttcaatatc caagtggtta gttggctcgt ccaagaccaa gagattgggc
    10021 ttttcaagga gcattttggc aagtgccaaa cgagtatttt gaccaccaga aagctcagca
    10081 attttcatct gccacataga ctcgtcaaac ttgaatccat tcaaaatcgc tcgaatatca
    10141 gcttcatagg taaagccacc tgcttggcga aaattctcag ataagcggtc ataatctgac
    10201 atcagtttat ccaaatactc accagacttt tcacccatct ccagctccat ctgacgcagt
    10261 tgtctctccg tccgacgcaa atcattaaag acatgaagca tttcatcgta gatggtattt
    10321 tcagactcaa aacggctatc ttgggctagg taagacagag aaatatcttt tttcttattg
    10381 atttctccgc tagttggctc ctcttctcca actaaaatct tcaaaagagt agacttacct
    10441 gcaccatttt tcccaacaag agcaatccga tctcgttcat caacctgcag gttgatatta
    10501 tcgaaaagaa cctctcctgc aaaagaacgt tcaattttat tagcttgtaa aataatcata
    10561 caagtagtat agcatgtttc cctaaggcat tcaagataat cgtaagtctt ttagtacaac
    10621 ttttataaca taaaataaac taaattatgt atattttata ttagattact tcactatctt
    10681 gttggatttt ctaaccagct aatcttgttt caaatagtta tcgcacaagt ctattattta
    10741 attcttttca tcatttacgt ac
//
LOCUS       DAVKNL010000063        10605 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVKNL010000063 DAVKNL010000000
VERSION     DAVKNL010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10605
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.063"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(173..697)
                     /locus_tag="VW411_001725"
     CDS             complement(173..697)
                     /locus_tag="VW411_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEU9794417.1"
                     /translation="MKIAIIGYSGSGKSTIAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFSPLTCLFRAFKRYL
                     KYRGKVRESMAVDCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(750..986)
                     /locus_tag="VW411_001726"
     CDS             complement(750..986)
                     /locus_tag="VW411_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEU9794418.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLVK"
     gene            complement(1054..1974)
                     /locus_tag="VW411_001727"
     CDS             complement(1054..1974)
                     /locus_tag="VW411_001727"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEU9794419.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEEKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            2164..2400
                     /locus_tag="VW411_001728"
     CDS             2164..2400
                     /locus_tag="VW411_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794420.1"
                     /translation="MKILKRYILELCFILSFALPFIKGTNADNGRCFVETYYGFTFLM
                     EHAIVTAVFICSFLIAFLLKNDGRNGLLRVVIAF"
     gene            2436..2567
                     /locus_tag="VW411_001729"
                     /pseudo
     CDS             2436..2567
                     /locus_tag="VW411_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820986.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
     gene            2783..3364
                     /locus_tag="VW411_001730"
     CDS             2783..3364
                     /locus_tag="VW411_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9794421.1"
                     /translation="MYYAENPDAAHDIHELRVDLLGEKMTFLTDAGVFSKKMVDFGSQ
                     LLLKCLDVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLAQRNAEKNNA
                     KATIFQSNIYARVQGRFDHVISNPPIRAGKQVVHEIIEKSKDFLEIGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            3361..4638
                     /locus_tag="VW411_001731"
     CDS             3361..4638
                     /locus_tag="VW411_001731"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEU9794422.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLIEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVHQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            4656..5318
                     /gene="deoC"
                     /locus_tag="VW411_001732"
     CDS             4656..5318
                     /gene="deoC"
                     /locus_tag="VW411_001732"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEU9794423.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKVVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            5305..5694
                     /locus_tag="VW411_001733"
     CDS             5305..5694
                     /locus_tag="VW411_001733"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU9794424.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMAEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            5784..6836
                     /locus_tag="VW411_001734"
     CDS             5784..6836
                     /locus_tag="VW411_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEU9794425.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALNNAVKDAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAERGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            6980..8515
                     /locus_tag="VW411_001735"
     CDS             6980..8515
                     /locus_tag="VW411_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9794426.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDISGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            8508..9566
                     /locus_tag="VW411_001736"
     CDS             8508..9566
                     /locus_tag="VW411_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9794427.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            9569..10525
                     /locus_tag="VW411_001737"
     CDS             9569..10525
                     /locus_tag="VW411_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9794428.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGVIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         3019 a         1892 c         2414 g         3280 t
ORIGIN      
        1 gagcagtttt gtgataaaat gtaattgttc catatgattc tttctaatga gttgttttgt
       61 cgcttttcat tataggtcat atgggacttt ttttctacac aaaaataggc tccataatat
      121 ctatagtgga tttacccact acaaatatta tagagcccaa aaaggaagcc ctttatgaat
      181 tgtaggactt ccttttctta tccagaaatt gatctagctc tctctgattt cgaagaatag
      241 tgactttatg tgaatattct tggcaaagtt tttggtaatt ttctttttga gttttgctac
      301 gcccatccca aagaatccat ctgataaact cccactcaaa gcgttcaggg caatctaccg
      361 ccatactttc tctgactttt ccacggtatt taagataacg cttaaaggct ctaaagagac
      421 aggtcaatgg cgaaaaattg agaaagatga tttggtcagc ttcttgcatt cgttcttggt
      481 agtagcacca agaataatta ccatcgatga cccaagcttt atgcttggtg agaaagtttt
      541 ttatctcggt taacatccat tcgcagtcac tgtcttgcca accaggttga aattggagtg
      601 tgtccatgtg cagttttgga atggagtagt agttagataa cttttctgct atagttgact
      661 taccagaacc agaatatccg ataattgcga ttttcatttt ctaccttttc ctatttggag
      721 acaaaaaaac agcctctatg gactgtttct tatttaacaa gtttagctga aagacgagct
      781 ttatcgcggc ttgctttgtt tttgtgaatc aaacctttag tttctgcttt atcgatagct
      841 gagctagcag cacggaaaag ttcttcagat gggtttgctt cgaaagcttt tatagcagta
      901 cgcatagctg atttttgagc tgagttcttt tcgttttgtt taacgttcaa ttcagcgcgt
      961 ttgatagctg atttaatgtt tgccaatgtt cttacctcca tatttactaa ctataccatt
     1021 atatatgaaa acttacgttt tgacaaggga aaattacttt tttaagtaaa tttcatcgat
     1081 ttcatggttc ttgcttttat gaagaatcac ttctgcacga tttctggttg gttcaatata
     1141 attttgcaga tttgtgagat tgatactgat ccagacctga tgggcaaagg actccacttc
     1201 cccaatcggc atctgagtaa aacgataata gtagctatca gggtcgtttt gggctagact
     1261 cagcattttc aagaaacggt ccagatacca actttcaata tcatccactc cagcatctac
     1321 atagatggaa aagtcaaaga agtcagtgat atagagacga tcgttttgtg gattttgaaa
     1381 gacattgatt ccctcaacaa ttacaaaatc agcagctttg acactttgtt tctcttcggg
     1441 tacgatgtcg taaacttcat gagaatagac aggaatatct acatcttgtc catttttgat
     1501 gcggtccaag aagttgagaa gagcttccat atcatagctt tcaggaaatc ctttacgatt
     1561 taaaatcccc tgctcaatca aggtttgatt gggatagaga aaaccatcag ttgtaaccaa
     1621 ctcaaccgta gcatctgtaa acgtacggga cagtaggatt tgaagtaggc gactggttgt
     1681 ggattttcca acggcaacac tcccagaaac cccaataata aaaggttgag atttactttc
     1741 acgttggagg aaaattcctt ttgaaaaggc taaatcttcc ttagttcgct tgtaaatctg
     1801 aatcaaatga gccaagggga gatagatatc tgtaacgtct tggagactga tttggtcatt
     1861 aaaactcttg atagattcca attcttcttc tgtcaaagga ggtgttgtct ttcgatgtaa
     1921 agattgccaa gtctggcggc tgattttttc aaaatgtaaa aattcgttgg tcatttgttt
     1981 tcccctagat attttgtcta tcataccata aaaagctaaa aaaataaagc ggtttcttga
     2041 atgtggtaag tcaatggtat atagtataag tgatcaattt attgatgaaa taggagatag
     2101 attctactga tttataagct tataaagggg gctttttagt tcacaactta taggaggtgt
     2161 actatgaaaa tcttaaaacg ttacatattg gaactctgtt ttattttaag ttttgcttta
     2221 ccttttataa aaggaaccaa tgcagataat ggtagatgct ttgtggaaac ctattacggt
     2281 tttacttttt tgatggaaca tgctattgta acagctgtct ttatttgttc gttcttaatt
     2341 gctttcttac taaaaaacga tggacgaaat ggattgctgc gggtagttat tgctttttag
     2401 tcctacggat tgcgacagag ggttatttct tccacatgtc actagaagat ttgatacgac
     2461 tttggacaag tttggaaatc ctgacataaa cgtatcagtt gggcttttat ctaaacattt
     2521 tgttaggaat tttgttgata ataaagtatt ttaaggttaa gcaatagaaa taataatctg
     2581 cctgattata gatgataaac tttttgtttt tatctggact gtgatattta tagttctaaa
     2641 tggtttatta tttataagtg ttgaaagaga ttgaagaaaa tggcttcgtg acttgctttc
     2701 ttcggtcttt ttcgttttat tatcaatttg taaacgattt acaatttgag tcaaattatg
     2761 gtaaaatagt gtcatgagta aaatgtacta tgcagaaaat cctgacgctg ctcacgacat
     2821 tcatgagttg agagtggact tgttgggaga aaaaatgact tttttgacag atgcgggtgt
     2881 ttttagcaag aaaatggttg actttggaag tcaactcttg ctcaagtgtc tggatgtcaa
     2941 ccaaggagag acagtcctag atgtaggctg tggttatggt ccattaggtt tgtccttggt
     3001 caaggtttac ggagttcagg cgaccatggt tgatattaac actcgtgccc tggatttagc
     3061 tcagagaaat gctgaaaaaa ataatgcaaa agcgacgata tttcaatcta atatttatgc
     3121 aagagttcaa gggcgatttg accatgtcat ttccaatcct cctatccgtg cgggcaagca
     3181 agtcgttcat gaaatcatcg agaaaagtaa agatttcttg gaaattggtg gagatttgac
     3241 catcgtcatc cagaaaaaac aaggagctcc aagtgccaag tctaagatgg aagatgtgtt
     3301 tggcaattgt gaaatcctta aaaaagacaa gggatactat atccttagaa gtgtgaaaga
     3361 atgagagcag ttgatttaat ccaaaaaaaa cgagacggtc aagaactgac ttcaagtgaa
     3421 attgaatggc tgatagaagg ctatgtatca ggaactgttc ctgattatca gatgtctgcc
     3481 tttgctatgg ctgtttattt taaaggaatg acgactcgag aaatctctga tttaacgatg
     3541 aatatggtta agactgggca agagtttgac ctgtcagcca ttgatggtgt gaaagttgac
     3601 aagcattcta ctggtggtgt cggcgataag gtaaccttga ttttagctcc tcttgttgct
     3661 agcttcggtg tgcctgtagc aaaaatgagt ggtcgtggtc ttggccatac aggcggaaca
     3721 attgataaat tggagtccat taagggctat caagtagaac gtagtcaaga ggattttatt
     3781 cgtcaggttc aggatattgg agtatctgtc attgggcaat cagaccagct ggtcaaagca
     3841 gataagcttc tctatgccct tcgtgatgtg accgcaactg tcgacacgat tcctttgatt
     3901 gcgagttcgg tgatgagcaa gaaaattgcg gcaggggcgg atgctatttt gctagacgtg
     3961 actgtcggtg agggtgcctt catgaagacg gttgatgagg cgcgtgagtt ggctcaaact
     4021 atggtagatc ttggtaaggt tgttggacgg aagacagtag cagtgattac ggatatgagc
     4081 cagcctttgg gacgagcgat tggaaatcgt ctggaaatcc ttgaagcatt ggagattttg
     4141 caaggtcaag gacgtcagga tattacccac tttatttgtg aattggctca aattatgctt
     4201 ggtttggcaa atgttaacaa aactgttgaa gaagttcacc aacatcttga gaatggtcaa
     4261 gcactggcta agtttgagga aatggtgcaa gcccaaggtg gagacttgga agacctctat
     4321 cgtcctgtaa atgtagctca tgtggtggaa atccctgctc aggaaacggg tgtcatttca
     4381 gctcttccag ctatggactt tggtctttat gccatgaggc tgggtgctgg tcgtgcagtt
     4441 aagtctgatg ccttggacta tgaaacagga attgtttttg aaaagaagat tggagactcc
     4501 gttcaaaagg gtgaaattgt tgcaaaagta tacacaaatg aaaaaattcc acctcaacta
     4561 gttacagatt tccaaaaatg tgttaaaata agtgatgaag tgaaaaaaat ccgtgaaatt
     4621 gtagaaatca tctcctaaat ggagggaaaa ccgaaatgaa attaaataaa tatatagatc
     4681 atacgctttt aaaacaagat gcaaagaaaa aacaaattga tagtttgttg tctgaggcta
     4741 gagagtatga ctttgccagt gtttgcgtta atccgacctg ggttgaacat gctaaaaaag
     4801 gacttgaagg cacagatgtt aaggtttgca cagtagtagg tttccctttg ggagcaacaa
     4861 cttcagccgt gaaagcattt gagacaaaag aagctatcca aaatggtgca gatgagattg
     4921 atatggtgat caatgttgga gctctcaaat caggtaattt agccttggtt gagtcagata
     4981 ttcgcgcagt agtggaagca agtggtgata agttagtgaa agtcattatt gaagcttgcc
     5041 ttctgacaga ccaagaaaaa gttgttgttt gccaattggc ccaaaaagct ggggctgact
     5101 ttgtcaaaac atctactggc ttttcaactg gtggtgctac gatagcagat gttacattaa
     5161 tgcgtgaaac agttggatct gatatgggtg tcaaggccgc cggtggagct cgttcttatg
     5221 cagatgctct tgcctttgtc gaagcaggtg cgacccgtat cggaacgtca gctggggtag
     5281 ctattttaaa aggagaattg gcagatggcg actactgagt tgattgaact ggcaattgaa
     5341 accagcaaac atgcctatgt cccctattct cactttccta tcggagcagt tttagtagct
     5401 aaagacggaa gtgtttacac gggagtgaat attgaaaatg ctagctatcc tttgaccaat
     5461 tgcggtgaga gaacagctat ttttaaggct atatctgaag gccaaagaga attttcagaa
     5521 ttgattgtct atggtcaaac tgaaaaaccg atctcgccat gtggtgcttg tcgccaagtg
     5581 atggctgaat tttttgaaca agatctaaaa gtgaccttag tcgcaaaaga taaatcgacg
     5641 gtcgagatga cggtcgggga attacttcca tactctttta cagacttaaa ctagtctgag
     5701 tcgctctctg agtggcacgg tccttgtggc caaccaatcc atacttgcaa catcgttgca
     5761 catcttattt aggaggttca gtaatgaaca agaaacaatg gctaggtctt ggcctagttg
     5821 cagtggcagc agttggactt gctgcatgtg gtaaccgctc ttctcgtaac gcagcttcat
     5881 cttctgatgt gaagacaaaa gcagcaatcg tcactgatac tggtggtgtt gatgacaaat
     5941 cattcaacca atcagcttgg gaaggtttgc aggcttgggg taaagaacac aatctttcaa
     6001 aagataacgg tttcacttac ttccaatcaa caagtgaagc tgactacgct aacaacttgc
     6061 aacaagcggc tggaagttac aacctaatct tcggtgttgg ttttgccctt aataatgcag
     6121 ttaaagatgc agcaaaagaa cacactgact tgaactatgt cttgattgat gatgtgatta
     6181 aagaccaaaa gaatgttgcg agcgtaactt tcgctgataa tgagtcaggt taccttgcag
     6241 gtgtggctgc agcaaaaaca actaagacaa aacaagttgg ttttgtaggt ggtatcgaat
     6301 ctgaagttat ctctcgtttt gaagcaggat tcaaggctgg tgttgcgtca gtagacccat
     6361 ctatcaaagt ccaagttgac tacgctggtt catttggtga tgcggctaaa ggtaaaacaa
     6421 ttgcagccgc acaatacgca gccggtgcag atattgttta ccaagtagct ggtggtacag
     6481 gtgcaggtgt ctttgcagag gcaaaatctc tcaacgaaag ccgtcctgaa aatgaaaaag
     6541 tttgggttat cggtgttgat cgtgaccaag aagcagaagg taaatacact tctaaagatg
     6601 gcaaagaatc aaactttgtt cttgtatcta ctttgaaaca agttggtaca actgtaaaag
     6661 atatttctaa caaggcagaa agaggagaat tccctggcgg tcaagtgatc gtttactcat
     6721 tgaaggataa aggggttgac ttggcagtaa caaacctttc agaagaaggt aaaaaagctg
     6781 tcgaagatgc aaaagctaaa atccttgatg gaagcgtaaa agttcctgaa aaataatgga
     6841 tggaagtcat ttcttaggaa gcggccaagg gccgcttttt taagagttga gacaaagtca
     6901 ttttgtctaa gtaaagcttg ctactagaca aactagcaag ttttattgaa ataaaataag
     6961 actctgaaag gaagagcaca tggcacacga aaatgtcatt gagatgcgtg atattaccaa
     7021 ggtgtttggt ggatttgttg ccaacgacaa aatcaacttg cacctacgaa aaggtgaaat
     7081 tcatgcactt ttaggagaaa atggggctgg taagtccacg ctaatgaaca tgttagcagg
     7141 ccttcttgaa ccaactagtg gtgaaatcgc ggtcaacggt caagttgtca atctcgactc
     7201 cccatctaaa gcagctagct tgggaatcgg gatggttcac cagcacttta tgttggttga
     7261 agccttcaca gtggctgaaa acatcatttt aggtagtgaa ttgactaaaa atggtgtgct
     7321 agatatctct ggagctagca aagaaatcaa ggctctttct gaacgttatg gcttagctgt
     7381 tgacccttct gccaaggtag cagatatctc agttggagcc caacaacgtg tagaaatttt
     7441 aaaaacactt tatcgggggg ctgatatcct tatctttgac gaaccaacgg ctgttttgac
     7501 tccatcagaa attgatgagt tgatggctat tatgaaaaat cttgtcaaag aaggaaaatc
     7561 aattatcttg attacccaca aattggatga aattcgagca gtttctgacc gtgttacagt
     7621 tatccgtcgt gggaaatcaa ttgaaaccgt tgaaattgca ggggctacca atgctgattt
     7681 ggcggaaatg atggtaggac gttctgtttc ctttaaaaca gagaagcaag cctctaaacc
     7741 aaaagaagtg gttttgtcta tcaaagattt ggtggtcaat gaaaaccgtg gtgttccagc
     7801 tgttaaaaat ctgtccttgg atgttcgtgc tggagagatt gttggtattg cggggattga
     7861 tggaaatggt cagtctgaac tgattcaagc cattacaggt cttcgtaagg ttgaatctgg
     7921 tagcattgag ctaaaaggag attcaattgt aggcttgcac ccacgtcaga ttacagaact
     7981 aagtgttggg cacgttccag aagaccgtca ccgtgatggc ttgattttgg aaatgatgat
     8041 atctgaaaat attgcccttc aaacctacta taaagaacca catagtaaaa atggaatttt
     8101 gaattattca aatattactt cttatgctaa aaagctgatg gaagagtttg atgttcgcgc
     8161 tgccagtgaa ttagttcctg cagctgcact ctcaggagga aatcaacaaa aagcaattat
     8221 tgctcgtgaa attgatcgag atcctgatct ccttatcgtt agccagccaa ctcgtgggtt
     8281 ggatgtcggt gccattgagt atatccacaa acgcttgatt gaagagcgtg ataatggcaa
     8341 ggctgtcctt gttgtcagct ttgaattgga tgagatttta aacgtctcag accgtattgc
     8401 cgttatccac gatggtaaga ttcaaggtat tgtatcacca gaaacaacca ataaacaaga
     8461 acttggtgtc ttgatggctg gtggaaactt gggaaaggag aagagtgatg tctaaaaaat
     8521 tacaacaaat ttcggttccc ttgatttctg tattcctagg aattttactc ggagccattg
     8581 tcatgtggat cttcggttat gatgctattt ggggctacga agaattgttc tatacagcct
     8641 ttggcagtct gcgtgggatt ggagaaatct tccgtgctat gggtcctctg gtcttgattg
     8701 gtcttggttt tgccgttgcc agtcgagctg gtttctttaa cgtcggactt cctggtcagg
     8761 ctttggcagg ttggattctc agtggttggt ttgccctgtc gcatccagat atgccccgtc
     8821 ccttgatgat tctagcaacc atcgtgattg ccttgattgc tggtgggatt gtcggagcga
     8881 ttccaggtat tcttagggcc tatctaggga cgtcagaggt tattgtaacc atcatgatga
     8941 actacattgt cttgtatgta gggaatgcct ttatccatgc tttccctaaa gacttcatgc
     9001 aaagtacaga ttcgaccatt cgtgttgggg ctaatgcaac ctatcagaca ccttggttgg
     9061 ctgagttgac tggtaactca cggatgaata ttggtatttt ctttgccatc attgccgttg
     9121 cagttatttg gttcatgctc aagaaaacaa ctcttggttt tgaaatccgt gcagttggtc
     9181 ttaatccaca tgcttcagaa tatgctggta tttctgccaa gcggactatt atcctatcta
     9241 tgattatttc aggtgccttg gcaggtcttg gtggagctgt tgaaggtttg ggaaccttcc
     9301 agaacgtcta tgttcaaggt tcgtcattag ctatcggatt taacggaatg gcggttagtt
     9361 tgcttgcggc caactcacca attggtatac tctttgcagc cttcctattt ggcgttctcc
     9421 aagttggggc tcctggtatg aatgcggcgc aggtaccatc tgagcttgtc agcattgtaa
     9481 cagcgtctat tatcttcttt gtcagtgttc attaccttat cgaacgcttt gtcaaaccga
     9541 aaaaacaagt taaaggaggt aagtaaagat gtctattata accttgctcc ccctcttggt
     9601 gtcttctatg ctgatttact cagcacctct catctttaca agtatcggtg gtgttttctc
     9661 tgaacgtggt ggtgtggtaa acgttggcct tgaaggaatt atggttatgg gtgccttttc
     9721 tggagttgtc tttaaccttg aatttgcaga acaatttgga gcagcaactc catggctatc
     9781 cttgcttgta gcaggattgg ttggtagtgt tttctctatc atccacgcag cagcgacggt
     9841 tcatttccgt gcagaccatg ttgtcagcgg tacggtattg aacttgatgg cgcctgcctt
     9901 ggctgttttc ttagttaaag ttctttataa caaaggacaa accgacaacc taagtcaaac
     9961 ttttggacgc tttgatttcc cagtcttggc aaatatccca gtgattggtg atatcttctt
    10021 caagtcaact agtctacttg gttatctggc gattgccttc tcattccttg cttggtttat
    10081 tctctttaag actcaatttg gtcttcgtct ccgctctgtc ggtgaacatc ctcaagcagc
    10141 ggataccttg ggaatcaacg tctacaagat gagatattta ggggttatta tttcaggttt
    10201 tctaggtgga attggcggag cgatttatgc gcaatcaatc tcagttaact tctcagtgac
    10261 aactattgtt ggacctggat ttatcgccct tgctgcgatg atctttggga aatggaatcc
    10321 aatcggtgct atgctttcta gtctcttctt tggactttca caaagtttgg ctgttatcgg
    10381 ttctcaattg ccgttcctac aaggagtgcc agcggtttat cttcaaattg caccttatgt
    10441 tttgacaatt cttgtcttag cagccttctt tggaaaagca gtcgcaccaa aagcagatgg
    10501 tatcaactac atcaaatcaa aataagcata caaaaaaacg tcagtccaat gactgaacta
    10561 caccccaaaa gttagacaga aaaaatctaa cttttggggt gtttt
//
LOCUS       DAVKNL010000064        10451 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVKNL010000064 DAVKNL010000000
VERSION     DAVKNL010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10451
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.064"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            152..820
                     /locus_tag="VW411_001738"
     CDS             152..820
                     /locus_tag="VW411_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9794429.1"
                     /translation="MVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHYRILV
                     LAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMAESFR
                     SGLEAISQTQQEIGLAIGLTPLQVFYYVVLPQATAVALPSFSANVIFLIKETSVFSAV
                     ALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRLRHAGFGNPST
                     LSRK"
     gene            780..1457
                     /locus_tag="VW411_001739"
     CDS             780..1457
                     /locus_tag="VW411_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9794430.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIGISILSVLLSMMFRTVMG
                     IIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFGLARNFNINISGETSAIIVFTLW
                     GTAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTASFWIYGTILILYFAVCYPISKLSTH
                     LEKHWRN"
     gene            1458..2216
                     /locus_tag="VW411_001740"
     CDS             1458..2216
                     /locus_tag="VW411_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9794431.1"
                     /translation="MSETILEIKELKKSFGDNPILQGLSLEIKKGEVVVILGPSGCGK
                     STLLRCLNGLESIQGGDILLDGQSIVENKKDFHLVRQKIGMVFQSYELFPHLDVLQNL
                     ILGPIKAQGRDKKEVTEEALQLLERVGLLDKQHSFARQLSGGQKQRVAIVRALLMHPE
                     IILFDEVTASLDPEMVREVLELINDLAQEGRTMILVTHEMQFAQAITDRIIFLDQGKI
                     AEEGTAQAFFTNPQTKRAQEFLNVFDFSQFGSYL"
     gene            2228..3099
                     /locus_tag="VW411_001741"
                     /pseudo
     CDS             2228..3099
                     /locus_tag="VW411_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(3186..3833)
                     /locus_tag="VW411_001742"
     CDS             complement(3186..3833)
                     /locus_tag="VW411_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9794432.1"
                     /translation="MNEIITLKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(3830..4528)
                     /locus_tag="VW411_001743"
     CDS             complement(3830..4528)
                     /locus_tag="VW411_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794433.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFQ
                     SIFYFTRQKRRFIE"
     gene            complement(4538..5290)
                     /locus_tag="VW411_001744"
     CDS             complement(4538..5290)
                     /locus_tag="VW411_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794434.1"
                     /translation="MKQFVQFYKKDFLAVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLDNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            complement(5290..6054)
                     /locus_tag="VW411_001745"
     CDS             complement(5290..6054)
                     /locus_tag="VW411_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794435.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKKYLAIVG
                     STVIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQFLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSAHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(6150..6553)
                     /locus_tag="VW411_001746"
                     /pseudo
     CDS             complement(6150..6553)
                     /locus_tag="VW411_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6733..7365)
                     /locus_tag="VW411_001747"
     CDS             complement(6733..7365)
                     /locus_tag="VW411_001747"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEU9794436.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            complement(7399..8100)
                     /gene="pyrF"
                     /locus_tag="VW411_001748"
     CDS             complement(7399..8100)
                     /gene="pyrF"
                     /locus_tag="VW411_001748"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEU9794437.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKILSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            8419..8511
                     /locus_tag="VW411_001749"
                     /pseudo
     CDS             8419..8511
                     /locus_tag="VW411_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516271.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(8846..9559)
                     /locus_tag="VW411_001750"
     CDS             complement(8846..9559)
                     /locus_tag="VW411_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9794438.1"
                     /translation="MLKRFLALVWLRCQIILSNKSILLQVLVPFAFTYFYKYLMETQG
                     KVNDQQALVLLMMCLPFSFSLAVGSPITIILSEEKEKYNLQTLLLSGVKGSEYILSTM
                     FLPFLLTFVIMGTTPLILGVTIVHTFNYITIVLLTSLSIILFYLLIGLTAKSQVVAQV
                     ISLPAMILVAFLPMLSGLDKTVAKITDYSFMGLFTKFFTKWEEFSWNKTLIPNLTLLI
                     WIVLLLTLITITIRKKKIS"
     gene            complement(9552..10391)
                     /locus_tag="VW411_001751"
                     /pseudo
     CDS             complement(9552..10391)
                     /locus_tag="VW411_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061739.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT         3447 a         2138 c         1726 g         3140 t
ORIGIN      
        1 aaaatatttt gtttggaaat ttcagaaatt ttatgtctat gataaaaatc cttataacgg
       61 caataaaaaa tagatattat ccaaagaaga ttttaagtgc tacaataact gtattatttc
      121 tagatgggag gttctatttt tggattgatc cattgttgaa caatatctac cactatatca
      181 aaaggcattc tttctgacct tgcatattgc agtttgggga attttgggat cctttctgct
      241 cggtttaatc gttagtatca tccgacatta tcgaatcctt gttttggcgc aagtagcgac
      301 agcctacatt gaattgtcac gtaatacgcc ccttttgatt caactcttct ttctctactt
      361 cggtcttccc cgaatcggga ttgtcctatc ttcagaagtc tgtgcaacgc ttgggcttgt
      421 ctttttagga ggctcctata tggcagaatc tttccgaagt gggctggaag ccatcagtca
      481 aacccagcag gagattggcc tcgctattgg tctgacacct ctacaggtct tttactatgt
      541 ggttcttccg caagcaacag cggtggcact cccctccttt agtgccaatg tcattttcct
      601 tatcaaggaa acctctgttt tctcagcagt ggctttggcc gacctcatgt acgtcgccaa
      661 ggatttgatt ggtctctact atgagacaga cattgcgcta gctatgttgg tagttgctta
      721 tctaatcatg ctgctaccca tctcactggt ctttagctgg atagaaagga ggctccgcca
      781 tgcaggattc gggaatccaa gtactctttc aaggaaataa tctcctgaga atcttacagg
      841 gattgggcgt tacgattggg atatccatcc tgtctgtcct cttatccatg atgttcagaa
      901 cagtcatggg aatcatcatg acctcccatt ctagaatcat acgattttta acacgattgt
      961 atctggaatt tatccgtatc atgccccagc tggtgctact cttcatcgtt tactttggct
     1021 tggctcgaaa ctttaatatc aatatctcag gtgagacttc agctattatc gtttttaccc
     1081 tctggggaac agctgaaatg ggagacttgg tacgtggagc tatcacttct ctccctaaac
     1141 atcagtttga aagtggacag gcactcggct tgactaatgt tcaactttac taccacatca
     1201 tcatcccaca agtcttaaga agactgctac cgcaggctat caatcttgtc actcggatga
     1261 ttaaaaccac ttcattagtt gttttgattg gggttgtgga agtgaccaaa gttggacaac
     1321 aaatcatcga tagcaatcgc ctgaccatcc caactgcttc attttggatt tatggaacca
     1381 ttctaatctt atatttcgca gtttgctacc ctatttccaa actatccact cacttagaaa
     1441 aacattggag aaactaaatg tctgaaacta tcttagaaat caaggaacta aaaaaatcct
     1501 tcggagacaa tcccatcctc caaggacttt ctctagaaat caaaaaaggg gaagttgttg
     1561 tcatcctagg gccatctggt tgtgggaaaa gtaccctcct tcgttgcctc aacggcttag
     1621 aaagtattca aggtggagat attcttctgg atggtcagtc tatcgttgaa aataaaaaag
     1681 attttcacct agttcgccaa aagattggca tggtctttca aagttatgaa ctctttcccc
     1741 atctggatgt cttacaaaac ctcatcctag gccctatcaa agctcaagga agggacaaga
     1801 aagaagtaac ggaagaagct ttgcaattac tagagcgtgt cggtttgctg gataaacaac
     1861 atagctttgc ccgtcaatta tctggtggac agaagcaacg tgttgcaatt gtccgtgccc
     1921 tcctaatgca tccagaaatc atcctttttg acgaggtgac tgcttcgctg gatccagaaa
     1981 tggtgcgtga ggtgctggaa cttatcaatg atttggccca agaaggccgt accatgattt
     2041 tagtaaccca cgaaatgcag tttgcccaag ccattactga ccggattatc ttcctcgacc
     2101 aagggaaaat cgctgaagaa ggaacagctc aagccttctt taccaatccg caaaccaaac
     2161 gagcccagga atttttaaac gtctttgact ttagccaatt cggctcatat ctataaagga
     2221 gattcttatg aaactattca aaccactctt aactgtttta gcacttgcct ttgcccttat
     2281 ctttatcact gcttgtagct caggtggaaa cgctggttca tcctctggaa aaacaactgc
     2341 caaagctcgc actatcgatg aaatcaaaaa aagcggtgaa ctgcgaatcg ccgtgtttgg
     2401 agataaaaaa ccgtttggct acgttgacaa tgatggttct taccaaggct acgctacgat
     2461 attgaactag ggaaccaact agctcaagac cttggtgtca aggttaaata catttcagtc
     2521 gatgctgcca accgtgcgga atacttgatt tcaaacaagg tagatattac tcttgctaac
     2581 tttacagtaa ctgacgaacg taagaaacaa gttgattttg cccttccata tatgaaagtt
     2641 tctctgggtg tcgtatcacc taagactggt ctcattacag acgtcaaaca acttgaaggt
     2701 aaaaccttaa ttgtcacaaa aggaacgact gctgagactt attttgaaaa gaatcatcca
     2761 gaaatcaaac tccaaaaata cgaccaatac agtgactctt accaagctct tcttgacgga
     2821 cgtggagatg ccttttcaac tgacaatacg gaagttctag cttgggcgct tgaaaataaa
     2881 ggatttgaag taggaattac ttccctcggt gatcccgata ccattgcggc agcagttcaa
     2941 aaaggcaacc aagaattgct agacttcatc aataaagata ttgaaaaatt aggcaaggaa
     3001 aacttcttcc acaaggccta tgaaaagaca cttcacccaa cctacggtga cgctgctaaa
     3061 gcagatgacc tggttgttga aggtggaaaa gttgattagt cattaactct taaaaggaac
     3121 tggattttaa gctccaatcc ctttttaaga ttttacctat aacatcctga gtctatctaa
     3181 gatgttcaat ctgaacacag tgtacatact ttatcttcta ttgcatatac tttatcacat
     3241 aagatacgaa tatcctcttc actatgacta gcaatcaaaa ttgttgtccc tttttcacta
     3301 gagagctttc taaacaatgt tctcatattt tctacacttg atttatccaa ggcattcata
     3361 ggttcatcta gtaaaagaat agagggattc tccataattg cttgagcaat ccctagcttt
     3421 ttcctcatac ctagcgaata agttttaact ttctggtctt tttgctcata tagaccaact
     3481 attttcagtg tatcattgat ttcctgatta ccaactactc ctcgtatgct tgccaaatat
     3541 tgtaaattct taaagccact ataataattt ataaaaccag gttcttcaat caaagctccc
     3601 aaattagctg gaatttttct ctcaggaaca atattttccc cattgattaa cacttctcca
     3661 taagacggac tatataaacc agctattaat ttaaacaata cacttttccc tgagccattc
     3721 gcaccagtaa ttcctataat ttccccctgt ttacaactaa agttaaggtt ttgaaaaaca
     3781 catgtctttt ttaatttcaa ctcaatattt tttaatgtaa ttatttcatt cattctataa
     3841 acctcctctt ttgacgagtg aaatagaaaa tgctttgaaa aagaaagact aaaaatagca
     3901 actgaagaaa taaatctcgt cctatatctc cattccctcg attcaaaata taaaatagat
     3961 aattagttcg atttcctaca aatagaccac caaacacaat catgagtaaa aagaaactaa
     4021 cgcaagcaaa gttcatttgt acattcgctc ctagtgcgta taacataaag caaaacaaga
     4081 ctaacacaca agcattcaaa gaatcaacta taaacaaaac tcctatatcc cagtttggta
     4141 actgtaacat aaattgaatc actagaactt ttaccatgat taaaatgagt ccataaagaa
     4201 aacaggtcca aatctgatac ctaatccaga gcttaataga agaaaatcga atcacttcca
     4261 taaagaaatc ggatgctctt ctacgatata caactgctag aaaaaatgta tgaataataa
     4321 accaatgaag caaccatata gataagacta aaaaagagtt ttgactcaga ttaggccctt
     4381 ttaaaatttc tataacaagt ctatcatttc cttctaacca ataccataat cctaaacaaa
     4441 tcaatatcca aaaaagaaca taaccccaaa agtacttggt atagattatc tgattcaacc
     4501 tccacatgtg actacttaac tttcccattt ccaactttta cactctcctc cagtttctat
     4561 gaacagaaaa tacagtcaat ggaattatga tagatagtaa tgtaagccaa taaatcaatg
     4621 catacggatt ggaatttacc atatactgaa aaattggcaa taatccaatt atcccattgt
     4681 ccaactgata aatccagaat aaggattcta caaataaaaa agtaaacaga aatactaagc
     4741 tcatgtttga ttttttaaac caagaaaaac tgctaatagt aagtaaggat aaaaagaaat
     4801 agtatgctat ataagagaaa aaaaatccta taaagaaact agcattgttt gcaatactta
     4861 taccataaaa ttctcttaaa aaatcaacct cctttatctc caaagagaag ctaaaaccat
     4921 tactaaatgc aattagaaaa atcaataaaa ataaagtcgc cgtccaaatc cccgtactaa
     4981 gagctgctaa tttgaaacta aaactggtaa agtgcttaat tgatttcaga cgaatgcgac
     5041 actccaacct attaaaatag ttattcatca aataaaaaaa gaacaatata tatgtgaacg
     5101 gaaagcaata tactccagtc gtcatatctt gaagtaaaac taagatccat tctaatacat
     5161 ttggatggat tgaatattgg cgacagcgca ataaatatac tgtactagat aaaacacagg
     5221 atagcagtaa tataaaataa accaatactg ctaagaaatc ttttttataa aattgaacaa
     5281 attgtttcat tatacatagt cctctgaatg tagaaaaaat gtaccataaa caaccaaaca
     5341 actaacaaat aaaataaaag caagatgagc actaactaag gaaagactga tatctttctg
     5401 atatcccaaa gctagcgttg tcacaggttc taagtaagat agccctaaaa tagcccaaaa
     5461 aataccacca accatcatat aggcaactgg gatgaaaata gctcctattt ttttcttcac
     5521 tagcaaagca ctagctagtc caaaaataga gaacacagcg ccccaaactc cataccagag
     5581 agtcgttaca agactataga gaaactgatt ggaatcaaat aattctttta aggcaccact
     5641 ataatctcca atataaattt cctgataagg agtcactaaa agattaattc ctaataataa
     5701 taaatagggg agaaaaaaga ctagaaaaga agaaataatt acagtactac ccacaatagc
     5761 cagatacttc tttttagaaa ttcgacacaa ttgtgctgtt agaaaatgac tctcagcatc
     5821 ctctattatc tgactagaat agggcagtgt acagataagt gcagctacta ggctaatcgg
     5881 tgaaaatcct cgaatagaag aagcggcaaa aaaaatcgat aaaccttcaa ttttataaat
     5941 accattgaaa gcaaggaaat ttcctaaact catgcaaaga agggtcaaaa ataaagacat
     6001 ataaaatcga ggtgattgaa cgactcgtac aagattacaa atgaaaaatt ccatccttac
     6061 tcctccttat aataaaaata gggtgtagca ttcttttttc atgctacacc cacaatcaac
     6121 catctttaag gcttactctg acaagtaagt taataagaat ctggattcca agaacctgaa
     6181 gtatgaattc ttacatgatt tccaaattgt ggcgccatag ctaatctagt accagaacca
     6241 atataattgt caccacctcc attatagtac atgacaatcc tagagccaga ccccaatgaa
     6301 tataccgggg taatatctga cccactatag gcgctacgaa tagaggtatt taacctttta
     6361 ccgccaccag tgctgtcatt attattaatt ccagcagagg agttttcttt ctcaagctca
     6421 ctagttgtat ccgaacccca acgcggcgac gttaaatcat aatctccaat ctgtctagct
     6481 aaaactgtag ggagaaacaa actagatgca tttgtaacta cgcaaaaaag gaatatattt
     6541 ttcttctttt catgacatcg tcctcttaag aagtgataaa caagatagtt gattgatcaa
     6601 tttgatacat attacatttt cattatatca acaaaagaaa gcgttgtcaa ttattttatt
     6661 taaaatattg tcatatttta aataaaatgg taaaagagac tcggtagcct cgtctctctt
     6721 tatcttactg acctaacctt cttgccaatt ttcttggtct tctttaaagc gttttagaag
     6781 atcaaggccc tctggcgtga tgtaaccttc ttcttgggct agatggataa gctcgctata
     6841 gtttgaaagc gtcacaagtt taacaccagc atctgcaaag ttcttatctg cttttggcaa
     6901 ttggtagctg aaaatcgcta caactccaag tacatctgct ccttctcgct tggctgctgc
     6961 tacagcttca agaactgaac cacccgttga aataaggtct tcaaccacta ccattttttg
     7021 accttgagct acgcgacctt cgatttgatt accagctccg tggtcttttg gtttactacg
     7081 gatgtaggca aaaggcaagt ccatcttatc agcaataatg gctccgtgtg gaatccctgc
     7141 tgttgcagtt cctgcaatca cttctacttc aggaaaggct tctttgatag cttccacaaa
     7201 accattttca attagggtac gagtttctgg ataggctagt gtcacacgat tatcagtgta
     7261 aatcggtgac ttgataccag atgcccaagt gaagggttcc tctggtttga ggtaaacggc
     7321 ttggattttc aagaggtggc tagcgatatc tttagcaagt gtcatggtat tctcctttta
     7381 tttttctaat ctagttcttt aattccagtc ctgtgtccat tcatccttga tggcatgata
     7441 agctgcaaca ggatcctcag cttgggtaat gggacgtccc actacgatat agtcactgcc
     7501 gatttgatag gcatcagcag gtgtcatgac tcgtttttga tctccaactg caacaccagc
     7561 tggacgaatc cctggtgtca gacagataaa atctggattg gtagcctgct tgatgacttg
     7621 tacttcctga gccgagcaaa caacaccatc caagccagct tcagctgtct tcttggcata
     7681 gtgaatcaca gactcttgca gactggtttg gatattttga aactcctgca tctgagcttc
     7741 tgacgttgat gtgagctgag ttacagcgat caatttggct tgactcccaa gaccttcacg
     7801 cgccgccttc atcatctcta caccaccagc cgcatggaca ttagtcatat cgacaccaag
     7861 ctgagacaag atcttcatgg ctgacttgac tgtattagga atgtcatgaa gtttgagatc
     7921 caaaaagaca ctatgaccca aaccttttaa gtaggacaca atctcaggcc ccgctgcgta
     7981 ataaagctcc atccctacct tgagataaag gctttcttct gctgggaaaa gagctaaaaa
     8041 ttccttgacc gcctcaaaac taggaaaatc aagagcaatg attggacgat gttctcgcat
     8101 aggtttctcc ttttaccttt actagtgaga gagtctggcc aacagaagcc acgaaaaaag
     8161 gaacctgcca acagcaaggt tccacgaaaa atgggtagtc tccacccttt caccgtctaa
     8221 ccttagctgc ctctctggac tgctttaaag gttttttact attctattat ttctactagc
     8281 acttgtcaag taaaaacagc taaatccatg ttgactgcta ttgaacacct cacaagactc
     8341 tttccaaaag aatagttaaa gactttcact tcggacagag aaaaagagtt tgcttgttac
     8401 cctttaattg aacaaatggg aattctgttt tattttgcgg atgcctattc atcttggcaa
     8461 aggggaagta atgagaatgc gaatggactt cttcgtgagt attttcttaa accgatttgg
     8521 caacaattac cgaaaaaatc cttaatgatg ctttgctagc ttattaatca ccgtccccaa
     8581 aaatacttgg attacaagac aggttttgaa gcactttttg aagcacttat agaggagatt
     8641 taaaaatgtt gcatttttta ttgcaattta tcagttatct tatcacccaa aacttctgca
     8701 attagtatga gcactattgt tatttttctt ttttcgtaca ctcaaatcaa aatatagcaa
     8761 taagactatt ctgtagaatt gctgtcaaaa agatcagttc attttttcct tcccacttgt
     8821 gtttataatc attaaaataa ctcaattaag aaattttctt tttcctaata gttatcgtaa
     8881 ttaaagttaa tagaagaaca atccaaataa gtagtgttag attaggaatt agagttttat
     8941 tccatgaaaa ttcctcccat tttgtgaaaa acttagtaaa tagtcccata aaactataat
     9001 ctgttatctt cgcaactgtc ttatccaaac cagatagcat cggtaagaaa gcaactaaaa
     9061 tcatagcagg aagactgata acctgagcta ctacttggct cttcgcggtt aaacctatca
     9121 ataaatagaa taaaatgatg gataaagagg ttagaagaac gattgtaata taattaaaag
     9181 tatgtacaat tgtaactcct aaaataagag gagtagttcc cataatcaca aaagttagca
     9241 aaaaaggaag aaacatagtt gataaaatgt attcggagcc tttaacacca ctcaacagaa
     9301 gagtttgtaa attgtacttt tctttttctt cagacaagat aatagttata ggacttccaa
     9361 cagccaaaga aaaagaaaaa ggtaaacaca tcatcaaaag aactaatgcc tgttgatcgt
     9421 tgaccttccc ctgtgtttcc ataagatatt tataaaaata tgtgaaagca aaaggcacta
     9481 aaacttgcaa taaaatactc ttattggaaa ggatgatttg acaacgcaac cataccaaag
     9541 ccagaaaccg tttaagcatt tagctttcct cctgttaatg tgataaaaat atcttctaaa
     9601 gtaggctcac atgaatgaat tgaaaaaata ttttccagat cagtctgaga tacctgttct
     9661 agtgatgtaa aatcaaaagt tatctgattc ccattataat ctgtaacctt aatcttttta
     9721 tctttattat atctttgaat gagttcagaa ggagctcctt gctcaactaa tttcccttta
     9781 tttaataagg caacataatc acataaaaga gttgcttcat tcatatcatg agtcgttaga
     9841 aaaatcgttg tccctgctgt tttcaattct aaaattaact catgaattgt tcgagaagtt
     9901 gtgggatcta gacctgaggt cggttcatcc agaaagagta cagcggggtt gttgataaga
     9961 gctcgtgcta aaagcattcg ttgcctcatt ccagtggata attttcctgc taccatcttg
    10021 cgactatcat ataatcctac tcgctttaac aaattatcaa cacgtgattt actaatatta
    10081 taaaatttac tataaaaaag aagattgtta tacagagaca ttttctcata aaatccactt
    10141 gtatcgctaa ccaatccaat tctctttaat tcaccgcttg ttaaattttg agatttttgt
    10201 cccaaaataa tagattgtcc tttatcggca aggaactgcc cagtcagaat attaatcgtt
    10261 gtggtctttc cagaaccaga tggttctaaa aatccaaaaa tctaaccttc tttaacagtg
    10321 aagcttatat tatttaaagc tattttatca ccaaaacttt ttgataaatg gctaacttca
    10381 atcatactca ttgcacctcc tttatattct acatttttac ttaacatcta tttacttatc
    10441 tttatcaaat a
//
LOCUS       DAVKNL010000065         9782 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVKNL010000065 DAVKNL010000000
VERSION     DAVKNL010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9782
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.065"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            <1..218
                     /locus_tag="VW411_001752"
     CDS             <1..218
                     /locus_tag="VW411_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choline-binding protein"
                     /protein_id="HEU9794439.1"
                     /translation="SWYYLNANGDMATGWVKDGDTWYYLEASGAMKASQWFKVSDKWY
                     YVNGSGALAVNTTVDGYGVNANGEWVN"
     gene            complement(448..1428)
                     /gene="dusB"
                     /locus_tag="VW411_001753"
     CDS             complement(448..1428)
                     /gene="dusB"
                     /locus_tag="VW411_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEU9794440.1"
                     /translation="MTNLNTPFMIGNIEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIETLLQLEKA"
     gene            complement(1415..2287)
                     /gene="hslO"
                     /locus_tag="VW411_001754"
     CDS             complement(1415..2287)
                     /gene="hslO"
                     /locus_tag="VW411_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEU9794441.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            2452..3879
                     /locus_tag="VW411_001755"
     CDS             2452..3879
                     /locus_tag="VW411_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9794442.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELAIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIHYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSAGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLVAIRQLLFT"
     gene            4038..5546
                     /gene="glpK"
                     /locus_tag="VW411_001756"
     CDS             4038..5546
                     /gene="glpK"
                     /locus_tag="VW411_001756"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEU9794443.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            5588..7414
                     /gene="glpO"
                     /locus_tag="VW411_001757"
     CDS             5588..7414
                     /gene="glpO"
                     /locus_tag="VW411_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEU9794444.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKSDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPAANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKHLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELTLSPVDFLLRRTNHMLFMRDSLD
                     SIVEPILDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            7484..8182
                     /locus_tag="VW411_001758"
     CDS             7484..8182
                     /locus_tag="VW411_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU9794445.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVFVSGKLSPAYLNPAVTIGVALKGGLPWASVLPYILAQFAGAMLGQILVWLQ
                     FKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAGIG
                     TFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVVGP
                     VIGAALAVLVFSLF"
     gene            8479..8682
                     /locus_tag="VW411_001759"
     CDS             8479..8682
                     /locus_tag="VW411_001759"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794446.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEGKKQELLNRLNK"
     gene            complement(8769..9356)
                     /locus_tag="VW411_001760"
     CDS             complement(8769..9356)
                     /locus_tag="VW411_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794447.1"
                     /translation="MFVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNILSDEVLE
                     VVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVNTIHGNKQ
                     ANTFANFMTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSPYPYYITS
                     TYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
BASE COUNT         2827 a         1939 c         2221 g         2795 t
ORIGIN      
        1 gctcatggta ctacctaaac gctaatggtg atatggcgac aggttgggtg aaagatggag
       61 atacctggta ctatcttgaa gcatcaggtg ctatgaaagc aagccaatgg ttcaaagtat
      121 cagataaatg gtactatgtc aatggctcag gtgcccttgc agtcaacaca actgtagatg
      181 gctatggagt caatgccaat ggtgaatggg taaactaaac ctaatataac tagttaatac
      241 tgacttcctg taagaactct ttaaagtatt ccctacaaat accatatcct ttcagtagat
      301 aatataccct tgtaggaagt ttagattaaa aaataactct gtaatctcta gccggattta
      361 tagcgctaga gactacggag tttttttgat gaggaaagaa tggcggcatt caagaggctc
      421 tttaagagag ttacgggttt taaactatta agccttctcc aattgcaaga gggtttcaat
      481 ctctgccagg gtgctggctt gcgaaatggc tccacggagt ttggcagcgc cagatgttcc
      541 acggagatag tgaggagcga gaccgcggaa ttcacgaact gcgacgtttt ctcctttgag
      601 gttaatcaat cgtttcaagt gttcgtaggc gatcttcatc ttgtcttcaa aggtcaaatc
      661 aggtaggatt tctcctgttt caaagtaatg gttgatttgg ttgaagaggt aaggatttcc
      721 catggcagct cggccaatca tgactgcgtc agcaccaact tcttcgatgc gttgcttggc
      781 ttcttggaca gtacggatat caccgttggc gatgaatgga atcttggtta gagcttgggc
      841 aaccttgtaa agggtctcaa ggtctgcgtg gccagtatac atttgttcac gggtacggcc
      901 atgcatggcg agggcagaaa cacctgcagc ttcagcagcg agagcatttt ctactgcaag
      961 agatgggtcc gcccagccgg tacgcatttt gacagtaagt gggatatcaa ggacagactg
     1021 gaccttgttg atgatggagt aaatcttgtc tggatccttg agccacatag caccagcttc
     1081 gttcttcacg attttgttga cagggcagcc catgttgata tcgacgatat cggtcttggt
     1141 gttttcttgg atgaattctg ctgcgcgtgc taggctgtct tcatcgctac caaaaagttg
     1201 gatagagaca gggttttcgc cctcatcgat atgaagcatg tgcagggttt tttcgttgtt
     1261 gtattggatt cccttgtcag agaccatttc cattacaacg agtccagctc cgagctcctt
     1321 tgcgatagta cgaaaggctg agttggtcac gccagccata ggcgctaaaa cggtacgatt
     1381 gggaatctca atattgccaa tcataaaagg tgtattaaga tttgtcacga atgagttcct
     1441 ccaggtcctt ttcatcaaag ttgtaagtag tttggcagaa ttgacaagtg atttctgccc
     1501 cgtggtcttc ctctttcatt tcctgtaagt ctgagcttgg aaggctggca agagcgttca
     1561 taaagcgttc atggctacag tcacattgga aacggatttc ttcttcagaa agacgcttgt
     1621 aggcttcgtc cccgtagata gccttgagga gggcttcgat atggtcgtcg ctttcgagaa
     1681 gagtagagat agctggcatt tcttggatgc gtttttcaaa gcgagcaatc tcttctttct
     1741 tggctcctgg caagacttga actaggaaac cacctgcaac cttgaccttg tcttcctcgt
     1801 ccaaaaggac attgaggccg accgctgaag gcgtttgttg gctttcagta aggtaaaagg
     1861 caaggtcttc accgatttct ccagagatga ggggagttat agagttgtaa ggatttccag
     1921 taccgtagtc tgtgataacg aggaattgac catttccaac aaaaggtccg actaggactt
     1981 caccagtcgc agtctttttg atgtcaacac caggattttg aacatagcct ttgacgttcc
     2041 ccttggtatc agcgacggtg ataatagcac ctagagagct agatcccaac accttaactg
     2101 taagtttggt atttcctttt tcattggctg cgagaatctg gctagcgata agagttcgac
     2161 caagcgctac agttgagcta gcttgggttt gatgtttttc ttgagcagtg cggacggttt
     2221 cagtgctatc aaggacaaaa gcacgaaagg ctccgctttc tgatatagtt ttaataattt
     2281 tatccatagc tactatttta gcataaaaat gcccaaaggg ggagccgtgt gtttactgat
     2341 tttcaggata atggaccagg aaatcagcat gaaaataaaa agagaaacag attattttag
     2401 catttgtcag atttatgcta tgcttaaggt agaaaatgaa agggataaca aatgtattta
     2461 ggagatttga tggagaaagc cgagtgtggt caattttcaa tactttcctt tctattacaa
     2521 gagtctcaga cgaccgtcaa ggctgtaatg gaagaaacag gattttcaaa agcaacccta
     2581 accaaatatg tcaccctgct caatgacaag gctttggata gtggcttaga gctggctatt
     2641 cactcagaag atgaaaatct gcgtctgtct atcggtgcag ctaccaaggg gagagatatt
     2701 cggagcttgt ttttggagag tgctgttaaa taccagattt tggtttatct tctctaccac
     2761 caacagtttt tagcccatca gctggctcaa gaattggtga ttagcgaggc tacgcttggt
     2821 cgtcacttgg ctggtttaaa tcagattttg tcagaatttg atttatccat ccaaaatggc
     2881 cgttggcgag gtccagagca tcagattcac tatttctatt tctgtctttt ccgaaaggtc
     2941 tggtcgagtc aggaatggga aggtcacatg cagaaaccag agagaaaaca ggagattgcc
     3001 aatttagagg aaatctgcgg tgcaagtttg tctgcggggc agaaattgga cttggttctc
     3061 tgggctcaca tcagtcaaca acgtcttcgg gtcaatgctt gtcagtttca agtcatagaa
     3121 gagaaaatgc gagggtattt tgacaatatc ttttatcttc gtttgctgag aaaggttccg
     3181 tccttttttg ctgggcaaca tattccacta ggagttgagg atggtgagat gatgatattc
     3241 ttctcttttc tcctatctca tcgcattctt cctcttcata ctatggagta tattcttggt
     3301 tttggagggc agttggcaga tttactgacg caattgattc aagaaatgaa gaaggaggaa
     3361 ctattggggg attatacaga ggaccatgtc acctatgaac tcagtcagct ttgtgctcaa
     3421 gtctatctct ataagggcta tattttacag gatcgctaca agtaccagtt agagaatcgt
     3481 catccatatt tactgatgga acatgatttt aaagagacag cagaggagat ttttcatgct
     3541 ctacctgctt ttcaacaggg gacagattta gataagaaga ttctctggga atggctccag
     3601 ttaatcgaat atatggctga aaacggtggc cagcatatgc ggattggtct ggatttgaca
     3661 tctggttttc ttgtcttttc aaggatggca gccattttga aacggtattt ggaatacaat
     3721 cgttttatta ccattgaagc ttatgaccct agtcggcatt atgatttgct ggttaccaat
     3781 aacccgattc ataagaagga acagacacca gtctattatt taaaaaatga cttggatatg
     3841 gaggatttgg tagcgattcg ccagttatta ttcacttaaa aggcttggtt aatccaggtc
     3901 ttttttgtga aattcacaca atctcctcac atttttttaa aaattaaaaa aagttgataa
     3961 acaagaaagc gctttatttt gtatactagt aagtgtaaag aggaaacacc tcaagatctt
     4021 tatcaggagg acagtacatg tcacaagaaa aatacatcat ggccattgac cagggaacta
     4081 caagttctcg tgccatcatt ttcaacaaaa aaggggaaaa ggttagctcg agtcaaaaag
     4141 agtttaccca gattttccct caggcaggtt gggttgagca caatgccaat gaaatttgga
     4201 actctgttca gtcagttatt gcgggtgctt tcatcgaaag tggtgtcaag ccaaatcaaa
     4261 tcgaggcaat cgggattacc aaccaacgtg aaacaacggt tgtctgggat aagaaaacag
     4321 gacttcctat ctacaatgct atcgtttggc agtcacgcca gacagcacct ttggctgagc
     4381 aactaaaaag ccaaggttat gtggaaaaat tccatgaaaa gactggtttg attattgatg
     4441 cttacttctc tgctaccaag gttcgttgga ttttggatca tgtagaaggt gctcaagagc
     4501 gagcagaaaa aggggaattg ctctttggta ctatcgatac ttggttggtt tggaaattga
     4561 ctgacggtgc ggctcacgtg actgactact caaatgcagc tcgtaccatg ctttataaca
     4621 ttaaagaact caaatgggat gatgagattt tggaaatcct taacattccg aaggctatac
     4681 ttccagaagt tcgttctaac tccgaaatct acggcaagac agctccattc catttctacg
     4741 gtggagaggt gccaatctca ggtatggctg gggaccaaca agcagccctc tttggacagt
     4801 tggcttttga gccaggtatg gttaagaata cttatggaac aggctctttc atcatcatga
     4861 atactgggga agagatgcag ttgtctgaaa acaacctctt gacaaccatt ggttacggaa
     4921 tcaacggtaa ggtttattat gccttggaag gttctatctt catcgcagga agtgctattc
     4981 agtggcttcg tgacggtctt cgcatggttg aaaattcacc agaatctgaa aaatacgctc
     5041 gtgattctca caacaacgat gaagtttatg tcgttccagc ctttacaggt ctaggcgctc
     5101 catactggaa ccaaaatgct cgtggttccg tctttggttt gactcgtgga acaagcaaag
     5161 aagactttat caaggcgact ttgcaatcta ttgcttatca agtgcgtgat atcatcgaca
     5221 ccatgcaagt ggatactcag accgccattc aagtactgaa ggtggatggt ggtgcagcca
     5281 tgaacaactt cctcatgcag ttccaggcgg atattttagg cattgacatt gcacgtgcta
     5341 aaaacctgga aacaacagct ctaggagcgg ccttcctagc aggtttgtca gtagggtact
     5401 ggaaagactt ggacgagttg aaactcttga acgagacagg agaactcttt gagccatcta
     5461 tgaacgaatc tcgcaaggaa caactctaca agggctggaa gaaggctgtg aaagcaactc
     5521 aagtctttgc ggaagtagac gactaatact ggcagaataa agcgatttat ttagaaagtg
     5581 tgtaaatatg gaattttcaa agaaaacacg tgaattgtca attaaaaaaa tgcaggaacg
     5641 taccctggac ctcttgatta tcggtggagg aatcacagga gctggtgtag ccttgcaggc
     5701 ggcagctagc ggtcttgaga ctggtttgat tgaaatgcaa gactttgcag aaggaacatc
     5761 tagtcgttca acaaaattgg ttcacggagg acttcgttac ctcaaacaat ttgacgtaga
     5821 agtggtctca gatacggttt ctgaacgtgc agtggttcaa caaatcgctc cacacattcc
     5881 aaaatcagat ccaatgctct taccagttta cgatgaagat ggagcaacct ttagcctctt
     5941 ccgtcttaaa gtagccatgg acttgtacga cctcttggca ggtgttagca acacaccagc
     6001 tgcgaacaag gttttgagca aggatcaagt cttggaacgc cagccaaact tgaagaagga
     6061 aggcttggta ggaggtggag tgtatcttga cttccgtaac aacgatgcgc gtctcgtgat
     6121 tgaaaacatc aaacgtgcca accaagacgg tgccctcatt gccaaccacg tgaaggcaga
     6181 aggcttcctc tttgacgaaa gtggcaagat tacaggtgtt gtagctcgtg atctcttgac
     6241 agaccaagtg tttgaaatca aggcccgtct ggttattaat acaacaggtc cttggagtga
     6301 taaagtacgt aatttgtcta ataagggaac gcaattctca caaatgcgcc caactaaggg
     6361 agttcacttg gtagtagatt caagcaaaat caaggtttca cagccagttt acttcgacac
     6421 aggtttgggt gacggtcgta tggtctttgt tctcccacgt gaaaacaaga cttactttgg
     6481 tacaactgat acagactaca caggtgattt ggagcatcca aaagtaactc aagaagatgt
     6541 agattatcta cttggcattg tcaacaaccg cttcccagaa tccaacatca ccattgatga
     6601 tatcgaaagc agctgggcag gtcttcgtcc attgattgca gggaacagtg cctctgacta
     6661 taatggtgga aataacggta ccatcagtga tgaaagcttt gacaacttga ttgcgactgt
     6721 tgaatcttat ctctccaaag aaaaaacacg tgaagatgtt gagtctgctg tcagcaagct
     6781 tgaaagtagc acatctgaga aacatttgga tccatctgca gtttctcgtg ggtctagctt
     6841 ggaccgtgat gacaatggtc tcttgactct tgctggtggt aaaatcacag actaccgtaa
     6901 gatggctgaa ggagctatgg agcgcgtggt tgacatcctc aaagcagaat ttgaccgtag
     6961 ctttaaattg atcaattcta aaacttaccc tgtttcaggt ggagaattga acccagcaaa
     7021 tgtggattca gaaatcgaag cctttgcgca acttggagta tcacgtggtt tggatagcaa
     7081 ggaagctcac tatctggcaa atctttacgg ttcaaatgca ccgaaagtct ttgcacttgc
     7141 tcacagcttg gaacaagcgc caggactcag cttggcagat actttgtccc ttcactatgc
     7201 aatgcgcaat gagttgactc ttagcccagt tgacttcctt cttcgtcgta ccaatcacat
     7261 gctctttatg cgtgatagct tggatagtat cgttgagcca attttggatg aaatgggacg
     7321 attctatgac tggacagaag aagaaaaagc aacttaccgt gctgatgtcg aagcagctct
     7381 cgctaacaac gatttagcag aattaaaaaa ttaagaaaaa ataaaagagg tggagggcag
     7441 cattccttgt cgcccgtccc ttctttttaa tggagacaga aagatgatga atgaattatt
     7501 tggagaattt ctagggactt taatcctgat tcttctagga aatggtgttg ttgcaggtgt
     7561 ggttcttcct aaaaccaaga gcaatagctc aggttggatt gtgattacta tgggttgggg
     7621 gattgcagtt gcagtctttg tatctggcaa gctcagtcca gcttatttaa acccagctgt
     7681 gaccatcggt gtggccttaa aaggtggttt gccttgggct tccgttttgc cttatatctt
     7741 agcccagttc gcaggggcca tgctgggtca gattttggtt tggttgcaat tcaaacctca
     7801 ctatgaggca gaagaaaatg caggcaatat cctggcaacc ttcagtactg gaccagccat
     7861 caaggatact gtatcaaact tgattagcga aatccttgga acttttgttt tggtgttgac
     7921 aatctttgct ttgggtcttt acgactttca ggcaggtatc ggaacctttg cagtgggaac
     7981 tttgattgtc ggtatcggtc tatcactagg tgggacaaca ggttatgcct tgaacccagc
     8041 tcgtgacctt ggacctcgta tcatgcacag catcttgcca attccaaaca agggagacgg
     8101 agactggtct tacgcttgga ttcctgttgt aggccctgtt atcggagcag ccttggcagt
     8161 gcttgtattc tcacttttct agtttatact cttcgaaaat caaattcaaa ccacgtcagc
     8221 gtcgccttac cgtactcaag tacagcttgc ggctagcttc ctagtttgct ctttgatttt
     8281 cattgagtat tagaaaacaa ttatgttgat agagcttggg caagagccca atttcagcaa
     8341 aaaatgaagt aaatcttctc ataataaaac gcatcatatc aagcacgaaa attccacgag
     8401 gtcaactaca gtcagaaagc tgaacaacaa gccaaaacgc ccaaaaaagg cggcaaaaag
     8461 caagcacctg caagcaacgt gccgaaatgg tcaaatcctg attatgtcaa cgaattagac
     8521 ccaaaaatcg ttgatatgct agtagaattt cacaagtcac aaggcacttt ggaaactccc
     8581 gaggcgcaag cagaaatcgc ccaaaaacgt gaagaaatcg agcaaaggag agctgagctt
     8641 gagggtaaaa aacaagagct tttgaaccgc ttgaacaaat agagtttcgc aagtattatg
     8701 cttacaaatt acttgagcaa ttaactaaaa tataaaccct gcctttatat ctaggcaggg
     8761 tttatatttt agaaattcac gtaggttgtt acggttttta catacccagt atagtttgag
     8821 tttctatagt attcagtgat aaacttccat tttctttgag caacatggat ataagtactt
     8881 gttatgtagt atggatatgg gctttgtgaa tccaagtaag actgataagc ttgtatacca
     8941 aaatatgctc caccaattat tgcaccccat ggacccccca ataaagcacc tatcctacca
     9001 atcatataac tgattccagc accagtcatg aagttagcga atgtgttagc ttgtttattc
     9061 ccatgtattg tgttgacgta attccaaaca ttaggatcgt atgatctaaa agatatattt
     9121 aggtcgattt cattcttttg ataagccata taaaatgccc cattgatata gacgccgtca
     9181 gcacgtcgtt caatagtgtc tacacttcca tctggattga caacctcaag aacttcatcg
     9241 cttaaaatat ttacttgcgt atctccgaac cgcactgatg agccattctc aaactgagcc
     9301 tcaccagata caactttaga gtttgccgat aagctatcat cagcaaaaac aaacaagcga
     9361 cggggaaatg ctagacatac agaaaacaga cataactagc aaacacatgc atttaaacat
     9421 cttagacata acggaaactc ctttgtattt ttgatttttt tcaactttta ttatacaata
     9481 aaaccaaata aaaagaaagc ggtaacaata tgcttaatgc gaaaattttt tatatatttt
     9541 tatgtttgat cgttatcgaa actacaggct tgttgttgtt gaaaagaggt ctcgaaatgg
     9601 gttatttaga cacagaagct attatcctcg cagttttttc atttgctttt tacaacctat
     9661 gttcattcgc ttgggtctgc tctacaataa aaaacaataa aaaataaata gacgtatttt
     9721 caaaaaaaaa aaaatgcata tttatattag caaaacgacg atttaaatcg tcgttttttt
     9781 gt
//
LOCUS       DAVKNL010000066         9726 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVKNL010000066 DAVKNL010000000
VERSION     DAVKNL010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9726
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.066"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            229..324
                     /locus_tag="VW411_001761"
                     /pseudo
     CDS             229..324
                     /locus_tag="VW411_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(577..1197)
                     /locus_tag="VW411_001762"
     CDS             complement(577..1197)
                     /locus_tag="VW411_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9794448.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLYTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTPNTWNDTSYQS"
     gene            complement(1197..1679)
                     /locus_tag="VW411_001763"
     CDS             complement(1197..1679)
                     /locus_tag="VW411_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEU9794449.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDQMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(1679..2521)
                     /locus_tag="VW411_001764"
     CDS             complement(1679..2521)
                     /locus_tag="VW411_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEU9794450.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDFKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDFLGTFFPIGIGAGATLIVFSKLIDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(2616..3416)
                     /locus_tag="VW411_001765"
     CDS             complement(2616..3416)
                     /locus_tag="VW411_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEU9794451.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDSQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            3560..4939
                     /gene="glmU"
                     /locus_tag="VW411_001766"
     CDS             3560..4939
                     /gene="glmU"
                     /locus_tag="VW411_001766"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEU9794452.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIASEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVIVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGNNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            4949..5494
                     /locus_tag="VW411_001767"
     CDS             4949..5494
                     /locus_tag="VW411_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9794453.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            5506..5820
                     /locus_tag="VW411_001768"
     CDS             5506..5820
                     /locus_tag="VW411_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEU9794454.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            5837..6529
                     /locus_tag="VW411_001769"
     CDS             5837..6529
                     /locus_tag="VW411_001769"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEU9794455.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNAQEQVVFGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQKSLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            6606..7097
                     /gene="rocS"
                     /locus_tag="VW411_001770"
     CDS             6606..7097
                     /gene="rocS"
                     /locus_tag="VW411_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEU9794456.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            7195..7785
                     /locus_tag="VW411_001771"
     CDS             7195..7785
                     /locus_tag="VW411_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEU9794457.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            7794..9557
                     /locus_tag="VW411_001772"
     CDS             7794..9557
                     /locus_tag="VW411_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HEU9794458.1"
                     /translation="MKPEKPKKLGLKQIYLDLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDRYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         3007 a         1683 c         2072 g         2964 t
ORIGIN      
        1 agttcatgta cacctgatat gatgcgtttt ataattttaa agactttttg accagcctca
       61 tttttttaac ttgatactca gtgaaaagca aagattaaac taggaagcta gctgtaggct
      121 gctcaaagaa cagctttgag gttgtagata aaacttgtga ggtcaccaac atatataatg
      181 tgaagctgac gtggtttgaa tagattttag aagagtatga gtctggaagt tttaatggat
      241 aatgcaagat tccatagaat gggtaagcta gagttcttat gtgaagagtt tgggcataaa
      301 cttttacctt ttcctcccta ctcatcttag tatagaaaag tgaatctgaa atagtacata
      361 actgcttcta aaacattctt ataaattgat ttaaattctc aaatcatatt attcagttct
      421 tatttcattt tgttctacaa tcctgttgag aagacacgtg ttcatatcaa aaaggtattg
      481 gcaagttgca ataccttttt acgaggctct gttgtcttat ttttgtttca actgactata
      541 tctcctatgg ttctagttca gaaggctagg ctataattat gattgataag aagtatcatt
      601 ccaagtattg ggagtgaatg tttcaaaatc atgggtttct ataatggtca ggctggcatt
      661 tgctagaccg ccatctttac gaagaagtgg ttctttatag cctaggagag tacgaagact
      721 ggcagtaaga ttggcgccgt gtccgacaat tagaatacgt tcagctggac tatcttttaa
      781 tgatttgata aattggatgg tccgttgagt tgtactatag agggattcgg ctccgaacat
      841 tcgagtgtca aattgagcaa gatttgaacg aaaagcctgg atttgttgcg ggtaaatagc
      901 ttccaaggtt gcaattttca aaccttctaa cttcccaagt tgccattcac ggagattagg
      961 aacgatttct aaagaacagg gggtatagag ttgactttgg ataatctcag cagatttgac
     1021 cgctcgaggt aaatcacttg aataaatctg atcaaaagga atttccttga gatactgacc
     1081 aagtcgtttt agggtttcaa tggattcagg aagaagagga gaatcaccac tagcaccttg
     1141 aaaacgacct tcttggttcc agagggtacg accgtggcgg acaaagtaga gtttcattac
     1201 ttgatgtcct ccaaaatatc tacaaagtct gcctttacaa agctagccaa gtcttgtggc
     1261 gcgacgataa tgctgtgtcc gacttcgcct gcagagacaa tcatttgatc caaatctaga
     1321 gcaattttat cgataaaaat gggataattg tgtttctgac gaattccgac aggattattg
     1381 gctccatgaa tgtaaccagt tgttttttct aagtcctttt gtggaatcat gctcactttt
     1441 ttattgccag aaattttagc tagtttcttt tcagacaagt gctgagtgat agggacaatt
     1501 ccgataatcg gtccggtctt gtctcccaaa agcgccaagg ttttgaaaat ctgatctcgt
     1561 tcataacctt gaggaagctc tccttctagg gcattgattt gaatcccctg atgagggata
     1621 gctgctttag ataggatttg ttccaccaat gtttttttga ttttaacttt ttttgccatt
     1681 atttatattt atcctccaat tgactcatcc aaataccaag ccagattccc agcgcaaaga
     1741 agaaggcgat gatgacataa ccgacaagtg aaagtcctgt gtattggata ctttcagcgt
     1801 ttcctgcatt tggaattaag atcaaaaggg tacttgatag gacgataccg atgatgaaat
     1861 gatagacgcg tgagtggtag ttgtttaagg cataatctat caattttgaa aaaacgatga
     1921 gagttgcacc tgcaccaatt ccaatcggaa agaaggttcc caagaaatca aaagttttaa
     1981 aaccagtcaa cataggagca tagagtccca aaatcaaaag taaatttgat gggctgaggc
     2041 caggaaccaa gacgccaagg gccaatagtg cgcctgctag gacgaagtta agaaagctgg
     2101 cgcttaaggt tccaacgaca aaatttaagg catagagtcc taatccagaa atgataaagg
     2161 ttgtccataa ccaagctaaa tcaatcttgt ctcggtcaga ttctcgagtt gattctttga
     2221 ggaggctagg aactgtaccg ataatagctc ccgcaaagct ccataataca aaaacctgat
     2281 aattttcaag caggtattca atcgggtagg aaaataagcc gattcccaga agcataccga
     2341 tggcaactgg aataaagtac aaaacatttt ctttaaagtc tttaaaggga tgggccagaa
     2401 agccaatcat tcgttcatag attcctaaga ttgctgctag aacccctccg gaaattcccg
     2461 gtaggataaa tccaagagca attacaatcc ctttaataac gcgtgctaac catgagagca
     2521 tatatttcct ccaactattt ctatttcaaa aaatccttac tatattgtat cacaatcaga
     2581 cacaaaaaga aaaagcaaat gataaacaaa tgcttttaag gttttaaaaa aagttttcga
     2641 aaggtttctt ctttattttt taagggagag ataacgttga tatctaaatc gtggtcaaag
     2701 ccggcaattt ttcctttaga tgtgtattgg tgaatatcat aatctaaatc agttttagga
     2761 ctgctctcca aaaatcctga gtctgagccg taggacggaa tccaaacaga ggtaaacttg
     2821 cctgtatcaa tactgtgttc ttccatgaag tagacaccaa cgtagatgcc gatgttttta
     2881 gcacccagtg atgctagttt tgctcgaaag ttttcgacac cttcgttcat attagacatg
     2941 gttttgtctt ccacgtcaag ccaatagtaa ctagggctgt aaggagaggc agcattgtag
     3001 aaaacttcgg cagccttttc catttcttgg acactttttc cagctacata agcgtagaca
     3061 gcaactggga cattccgctt ttgaagttca gtgatatgac tcttataggc cttgtctatt
     3121 ccattgataa atgaagcatc attttctttt gtcgtttgag caccactgtg aacacgaaca
     3181 atagcacctg aaatattttg tgagagggca tcgtagttga tttcctcagg acgctgccag
     3241 ccagagaggt caataatcgg tttgtctaag tgtttcaaag cctgtgcttc aatctgtgct
     3301 atattggatt ttgttttaaa cgattggctg tcattaagtg ggcgattgat gattaaaatg
     3361 aacatcataa tcccaaaaaa actaaataaa ataagtggat gaatttgttt tctcatatct
     3421 tataattcta ccctaaaaat caaaaaaaat caaaaaaatg ggttaaggaa gagactttag
     3481 agcatttttt cattcaagag tgcggaatga tttgaaatat ggtataataa aagggaattt
     3541 ctacagaaaa gagaagatta tgtcaaattt tgccattatt ttagcagcgg gtaaagggac
     3601 tcgcatgaaa tctgatttgc caaaagtttt gcacaaggtt gcgggtattt ctatgttgga
     3661 acatgttttc cgtagtgtgg gagctatcca acctgaaaag acagtaacag ttgtaggaca
     3721 caaggcagaa ttggttgagg aggtcttggc tggacagaca gaatttgtga ctcaatctga
     3781 acagttggga actggtcatg cagttatgat gacagagcct atcttagaag gtttgtcagg
     3841 acacaccttg gtcattgcag gagatactcc tttaatcact ggtgaaagct tgaaaaactt
     3901 gattgatttc catatcaatc ataaaaatgt ggccactatc ttgactgctg aaacggataa
     3961 tccttttggt tatggacgaa ttgttcgtaa tgacaatgct gaggttcttc gtattgttga
     4021 gcagaaggat gctacagatt ttgaaaagca aatcaaggaa atcaacactg gaacatacgt
     4081 ctttgacaac gagcgtttgt ttgaggcttt gaaaaatatc aataccaata acgctcaagg
     4141 cgaatactat attacagacg tcattggtat tttccgtgaa actggtgaaa aagttggcgc
     4201 ttatactttg aaagattttg atgaaagtct tggggtaaat gaccgtgtgg cgcttgcgac
     4261 agctgagtca gttatgcgtc gtcgcatcaa tcataaacac atggtcaacg gtgttagctt
     4321 tgtcaatcca gaagcaactt atatcgatat tgatgttgag attgcttcgg aagttcaaat
     4381 cgaagccaat gttaccttga aagggcaaac gaaaattggt gctgagactg ttttgacaaa
     4441 cggtacttat gtagtggaca gcactatcgg agcaggagcg gtcattacca attctatgat
     4501 tgaggaaagt agtgttgcag acggtgtgat agtcggtcct tatgctcaca ttcgtccaaa
     4561 ttcaagtctg ggtgcccaag ttcatattgg taactttgtt gaggtgaaag gatcttcaat
     4621 cggtgagaat accaaggctg gtcatttgac ttatatcgga aactgtgaag tgggaagcaa
     4681 cgttaatttc ggtgctggaa ctattacagt caactatgac ggcaaaaaca aatacaagac
     4741 agtcattgga aacaatgtct ttgttggttc aaattcaacc attattgcac cagtagaact
     4801 tggtgacaat tccctcgttg gtgctggttc aactattact aaagacgtgc cagcagatgc
     4861 tattgctatt ggtcgcggtc gtcagatcaa taaagacgaa tatgcaacac gtcttcctca
     4921 tcatcctaag aaccagtagg agcctatcat ggagtttgaa gaaaaaacgc ttagccgaaa
     4981 agaaatctat caaggaccaa tatttaaact ggtccaagat caggttgaat taccagaagg
     5041 caagggaact gcccaacggg atttgatttt ccacaatggg gctgtctgtg ttttagcagt
     5101 aacggatgaa caaaaactta tcttggtcaa gcagtaccgc aaagctatcg aggctgtctc
     5161 ttacgaaatt ccagccggaa aattggaagt aggagaaaac acagcccctg tggcagctgc
     5221 ccttcgtgaa ttagaggaag aaacagccta tacagggaaa ttagaactct tgtacgattt
     5281 ttattcagct attggctttt gtaatgagaa gttaaaacta tatttagcaa gcgatttgac
     5341 aaaagtggaa aatccgcgtc cgcaggatga ggatgaaacc ttggaagtcc ttgaagtgag
     5401 cttagaagaa gcgaaagaat taatccaatc aggtcatatc tgtgatgcca agacaattat
     5461 ggctgttcag tattgggagt tgcagaaaaa atagaggagg tcagtatggg taaatcttta
     5521 ttaacggatg aaatgattga aagagctaat agaggcgaaa aaatttcagg tcctcctttg
     5581 ctagatgata atgaggaaac taagatttta ccaacctctt cttcccgttt tggttatgcc
     5641 aatcctaagg atcatggttt tagccaggaa accttgaaga ttcaggtcga accatctatt
     5701 cataaaagcc gtcgtattga aaataccaag agaaatgtct tcaattctaa gttgaataaa
     5761 atcttatttg cggtcatctt tctcttgatt ttgcttgttt tagcaatgaa acttttgtaa
     5821 tagaaaagga attgaaatga aaataggaat tattgctgct atgccagaag aactggctta
     5881 tctggtccag catttagata atgcccagga gcaagttgtt tttgggaata cctatcatac
     5941 aggaaccatt gcttctcatg aagtcgttct tgtagaaagt ggaattggta aggtcatgtc
     6001 tgctatgagt gtggcgattt tggctgatca tttccaggtg gatgccctta ttaatacggg
     6061 ttcagctggg gcagtagcag aaggtatcgc tgttggggat gtcgtgattg ctgacaaatt
     6121 agcctatcat gacgtggatg tcacagcttt tggctatgct tatggacaaa tggcgcaaca
     6181 accgctttat ttcgaatcag acaaaacctt tgttgctcaa atccaaaaga gtttatctca
     6241 attggaccaa aactggcatc ttggtttgat tgctacagga gatagttttg ttgcaggaaa
     6301 tgacaagata gaagcgatta agtcccattt cccagaagtt ttagccgtgg agatggaggg
     6361 ggcagctatt gctcaagcag cgcatgccct caatctccca gtcttagtca tccgagctat
     6421 gagtgacaat gccaaccatg aagcaaacat cttttttgat gagtttatta tcgaagctgg
     6481 acgtcgctct gcccaagtct tgttgacctt tttgaaggct ttagattaag cggaaatttg
     6541 acagtttttc tagcttatga taagatttaa gtaaagaaaa gctagaaaac gtttcagagg
     6601 atattatgag tattgaaatg accgtcagtg agattgcaga ggtcttagga ttatctcgcc
     6661 aagcaatcaa taaccgtgtc aaagaattac cagaagaaga cacagataaa aatgacaaag
     6721 gggtaacagt tgttaccaga agtggcttga ttaagctaga agaaatctat aaaaaaacga
     6781 tttttgaaga tgagcctgtc agtgaagatg tcaaacaacg tgaactgatg gagattctag
     6841 tggatgagaa gaatgcagaa attcttcgtc tttatgaaca attaaaagcc aaggatcgtc
     6901 agttatcaga aaaagacgag cagatgcgta tcaaagaccg tcagattgct gagaaagaca
     6961 aacaattaga tcagcaacaa caattgaccc ttcaagccat gaaggatcaa gaaaatctta
     7021 agctagagct ggaccaagca aaagaagaag tccaatccac taaaaaaggc ttttttgctc
     7081 gtttatttgg aggataatca aggagctgtt taggttaaat gctaacctga tggcttcttt
     7141 tgtttgtgaa tagtatagtt gattagaaca gagacagtag aggagaggta cagaatggaa
     7201 cgattagaag attacattgc ttttgattta gaatttaatc agcacgaagg acaaacacac
     7261 ttgattcaag tatcggcagt gcgttttaga gatggtcggg aaatcgatgc ctatgattcc
     7321 tatgttcata ccagcgtccc tctaaaaagc tttatcaatg gtttgaccgg gattacagct
     7381 gagaccttaa aagatgctcc tccagttgag aaagtcttag aagaattcca agagtttgtg
     7441 ggttctttgc tgatgatagg ctacaatgct gctaaaagtg atttacctat tttagcagag
     7501 catggtttag actatagcca acagtacaag gtggatttgt atgatgaagc ttttgaacgt
     7561 cggagttcag atttacatgg tattgccaat ctcaaattgc aaactgttgc ctcatttctt
     7621 ggatttcaag gtcagtctca taatagttta gaggatgctc ggatgacggc gcgtgtttac
     7681 gaatccttct tagagtcgga tcaagctagg gaattgttag agacagaaag tagcttatca
     7741 aacaatcctt ttggtggctt ggacttgtct caattatttg actaggagtg cctatgaaac
     7801 ctgaaaaacc taaaaaattg ggtcttaaac agatttacct cgatcttgat aaaatcctct
     7861 ttctattttt cttgattttt atgatagtgg agtatttgtg ggtaccattc aattcatggt
     7921 tggctagctt cttattagat cagacaggtt atctttttat ttcctataac aatgtcttag
     7981 ccatccttac tcattctcca ttgattagtc tagctttttt agccttgatt gtaattaacc
     8041 ttctggttgc ttattttcaa ataggactcc tctttattgg tgctcgtcat ctcctctata
     8101 atgaaaagcg aaggctaatt gagtatatcc gcaaggtttt ccatgaaagt tttggattta
     8161 tgaaaaagct aacttgcgct aaagccttgt ttatcttttt ctatatagct atgctgtttc
     8221 cttttatacg gaaaatgctg aagatttatt atcttaataa aatcgtcatt ccagagttta
     8281 tccaagctta tttagaagat agatattgga tgtggtggct gagcatccta cttctatctc
     8341 ttatctttct ctatgtctct gtcagattga tgtttgccct tccgaagatt gtatatgatc
     8401 agctgactgt tcgagaagca gtgatgttta gcttggagaa aaccaagaag agagttactt
     8461 tttatgcttg gaatttattt tacatcttgc tcaaagcaca tttattattt tatcttcctt
     8521 tgattccttt attattggct caaactttgg tagatgatat cactcagaga gagtctctga
     8581 ttcttggtat tttgaatttt gtcgtgatta aaaatctcta ttatatggct ctgacttatt
     8641 ttttggttaa atttgtttca tttttgacag gaaaggagct ggatatgctt cctaggagag
     8701 aaaaagatca catcgtgcga tggggtgtca tgacttgtgc cagtcttttc tttgccttag
     8761 aaggttatat ttatctggag gctcccatgg ttcatctacc tcaacttatt tctcaccgag
     8821 gggtttccaa tgcaaatggg attcaaaata cagtagagtc cttggaaact acagcacaac
     8881 tcaaaccaga cttggtggag acggacgtgc aggaaacaaa agatgggcag tttgtcatga
     8941 tgcatgatgc taacttgaaa aatctagcag gtatcaataa aagtcctcaa gacttaaact
     9001 tggaggagct taaagggatt gatatttttg aaaatggcta ccagactaaa atttcaagct
     9061 ttgaagatta tctcagtcga gccaacgaac ttggtcaaaa attactaatt gaaattaaaa
     9121 ccagtaaaaa agatagtcca gacatgatga accgcttttt agcccgttat gctgcaaagc
     9181 tcaagattta tggacatcaa atccagtctt tagactacca tgttgtcgaa aaagtaagac
     9241 agtatgatgc cgaactacca gtttatttca tcatgcccta caattctgtc tttcctaaaa
     9301 caagagcgac aggatatact atggagtact caaccttgga tgaatatttt gtaagcaaac
     9361 tatggacaac ggatcagaaa ctttatgtct ggactgtcaa tgattcagaa gctatcagca
     9421 aatctcttca cttaggagta gatggagtga ttacagatga tttggaaaaa gttcagcggg
     9481 aaatagaagt agcccaagaa gatccagaat atacggattt gctcttgaaa aaagcattgg
     9541 aattctttga attttagtaa taaaaaagag gaacggttgt tcctcccatg ataggtaagt
     9601 attcttgtga atgcttgctt ttttgtggtt gcaatccgtt tacgagaatt gtttgtattg
     9661 ttcttgtttc aatttacttt atagtgcttt gaagttggaa tgctacacta taacttataa
     9721 aatata
//
LOCUS       DAVKNL010000067         9686 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVKNL010000067 DAVKNL010000000
VERSION     DAVKNL010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9686
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.067"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(408..1184)
                     /locus_tag="VW411_001773"
     CDS             complement(408..1184)
                     /locus_tag="VW411_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEU9794459.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGINALHPEDLIDYQKVFLNHHINCYF"
     gene            1306..2052
                     /locus_tag="VW411_001774"
     CDS             1306..2052
                     /locus_tag="VW411_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9794460.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYETKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRVIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     CQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAVSDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            2065..3045
                     /locus_tag="VW411_001775"
     CDS             2065..3045
                     /locus_tag="VW411_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEU9794461.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNVGIVKIEIENFDTNMFKLENYVKEKYSLESLEIIPNEFDDTPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            3240..3560
                     /locus_tag="VW411_001776"
     CDS             3240..3560
                     /locus_tag="VW411_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9794462.1"
                     /translation="MEMIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            3606..3914
                     /locus_tag="VW411_001777"
     CDS             3606..3914
                     /locus_tag="VW411_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9794463.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQSSGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            3931..5229
                     /locus_tag="VW411_001778"
     CDS             3931..5229
                     /locus_tag="VW411_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9794464.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASANLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVITQLVILAMSTLVYFPFFKVQDRLAYQNEIKQS"
     gene            5380..7818
                     /locus_tag="VW411_001779"
     CDS             5380..7818
                     /locus_tag="VW411_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEU9794465.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPSIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMTIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGRFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            7837..8505
                     /locus_tag="VW411_001780"
     CDS             7837..8505
                     /locus_tag="VW411_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEU9794466.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHKIRRQAGDDIFIKVPVTPAGLRAIKALK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVIQN
                     SRSI"
     gene            8523..9611
                     /locus_tag="VW411_001781"
     CDS             8523..9611
                     /locus_tag="VW411_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEU9794467.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNCPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
BASE COUNT         3196 a         1343 c         1955 g         3192 t
ORIGIN      
        1 atattagagt aaaattctct acaaaaagaa gaatagccta ttttactatt cttctgagtg
       61 atttcaattc ctttggggaa atatggagat actttttaaa tcctgacaaa tggttgtttc
      121 tttttctaaa tcggtgatac tgtatcggag aatgcgcgtg aggtcacaaa ggctgcgata
      181 gagcttctat ggagaatttc tttttggaga gattttttaa aggaatgaga catccgctac
      241 ctccttggaa ggtttttttg tgataagtat agcagtttaa aaattctaga gcaaggtaaa
      301 atagaaaagg tttggctaaa attttttagc taaacctttt gaaaagttaa gtatgtatta
      361 tagatgcttg ttatctgcaa atagctagag catttcaagg aaataaacta gaaataacaa
      421 ttaatatggt ggttcagaaa taccttttga taatcaataa gatcttcagg atgaagtgcg
      481 ttgattccat ccatttcata tttccgattt aataaacgat atttgttttc accaaattga
      541 tgaaaaggga gtagttgaac ttggtcgata tttaatgagt taaatagagt agcgaatttt
      601 tctgcatcct ctaaactatt gttaaaatta ggaataactg ggattcttaa aacgatagtt
      661 ttattttgtg aaaaagcata atgaatgttt ttaataatca tttgattaaa aaccccagtc
      721 acttttttat gttttataga attataatgt tttaggtctg tgtagataaa atccacatat
      781 tgaattaaat caataaattt ttcatgatca acaaaggcag tagtttcaat ggcagtgtgt
      841 atgtgatgtt ctttagctga ttttaagatg gctttagcaa attcaaactg agcaaatatt
      901 tcacctcctg ataaagttaa acctccaccg gattcttcgt aaaattcttt gtcttttaat
      961 acctctgtaa taatttcttc tacagtcttt tcttcaccga ctaaggtgaa tttctctcgt
     1021 tgagcatctt tcattttttc aggtttcatt ctttgagatt caggattaga acaccatgga
     1081 cagcgcagag gacatccttt taaaaaaaca gttgtacgaa tacccggacc gtcatgaatt
     1141 gaaaagtgtt gaatattaaa aataattcct ttagatattt ccataagagt tcctcctctt
     1201 cataagtcta tcctatcaca aaaacgaaag aaaaacaatt acgaacgaaa ggttaatttg
     1261 ttgttttttg ctttttattt tggtagaata aaacggaggt gtaaaatgaa aagattggaa
     1321 caaattatta aattagtatc agaatatgaa aagatcgacg ttaatacatt atcggaaaaa
     1381 ttaaatgtat cgaaagtaac gattagaaaa gatttagata aattagagtc aaaaggttta
     1441 ttacacagag agcatggata tgctgtatta aatagtggag atgacttaaa tgtacgtttg
     1501 tcaattaatt atgaaactaa gagaaaaatt gttcaggaag cagtaaaatt ggtgtcagat
     1561 aatgaaacaa taatgataga atctggatcg acctgtgctt tacttgctga ggaaatttgc
     1621 aagcaaaaaa gaaatgttac gattgtaaca aattcgtttt ttatagcaaa ttttgtgaga
     1681 gcttatgatt catgtcgtgt tattgttctt ggtggtgagt ttcagaaaga ttcacaggtg
     1741 actgtaggac ctttattaaa agaaatgata cagacttttc atgtgtgtca agcttttgtt
     1801 gggacagatg gttacgataa agagatgggc tttaccggaa aagatttaat gcgcagtgag
     1861 gtagttcaat atatttcagc agtgtcggat aaagtcattg tcctaactga ctcaagtaaa
     1921 tttgataaaa gaggtacagt aagaagattt gctttaagtc aagtctatga agtaataaca
     1981 gacgaaaaac tttctaaaca aaatatagct acattagaaa atgctgggat aatggttaag
     2041 gtagtttcgt aagaggttaa gtgtatgaat caagatagga ataaactgct ttctaaaatt
     2101 gcttatctgt attatattga aaacttaaat cagtcacaaa tagcagcaaa attaggaatt
     2161 tatagaacct ctattagtag aatgttaaca gaagcaagga atgtaggaat tgttaaaatt
     2221 gaaatagaga attttgatac caatatgttt aagttggaaa attatgtaaa agaaaaatac
     2281 agtttggaaa gtttagaaat tattccaaat gaatttgatg atactccaac aattttatct
     2341 gaaagaattt ctcaagttgc agcaggcgtc cttaggaatc taattgatga taatatgaaa
     2401 attggctttt cttgggggaa aagtttaagt aatttagtag atttaattca cagtaaaagt
     2461 gtccgaaatg ttcacttcta tcctctagca ggtggtccta gtcacataca cgctaaatac
     2521 catgtgaata cactgattta tgaaatgtct agaaaatttc atggagagtg tacatttatg
     2581 aatgcaacga ttgtgcaaga aaataaattg ttagcagatg gtattttgca atcaagatat
     2641 tttgaaaatt tgaaaaatag ttggaaagat ttagatatag ctgtagtcgg aattggtgat
     2701 tttagcaata aagggaaaca tcaatggtta gacatgctta cagaggatga ctttaaagaa
     2761 ttgaccaaag ttaaaactgt aggagaaatt tgttgtcgat tttttgattc aaaaggtaaa
     2821 gaagtctatg aaaacttaca ggaaagaacg atagcaatct ctttggaaga tttaaaaaat
     2881 attcctcaaa gtttagctgt tgcttacggt gatacgaaag tatcttcgat tctttctgtc
     2941 ttgcgtgcta atttagtaaa tcatttgatt acagacaaaa atacaatttt aaaagttttg
     3001 gaagaagatg gggatttgac ttttagagag attctaggtg agtgaaaatg atagactgat
     3061 tcagtttatc gtttttcttt ttagttgatt gcacatttgt gcttatataa acaaaaatag
     3121 tttatctgtt gtttttggat tgacaacttt attatgtagt tgtattctat agttacaaaa
     3181 gaaaatttta aaatttcaaa tgaaaaaagc tttttacata gtgaaatgag gaggaattta
     3241 tggaaatgat tgttccagat caaattatca tgggtttaat tttatatgct ggtgatgcga
     3301 aacaacatat ttataaagcg ttagattaca taaaaaatgg tacatgtgaa cggtgtgaag
     3361 aagaaataca gttagctgat gcagccttat tagaagctca taatctacaa acaaaatttt
     3421 tggcacagga agcgtctggt acaaagacag aaattacagc tctctttgtt cattcacaag
     3481 atcatctcat gaccagtatg acggagatta atttaatcaa agaaattatt agtttgagaa
     3541 aagaacttca taaaaaataa tactagagta ttatcattgt tattaacata gaggaggaaa
     3601 acataatggt gaagattggt ttgttttgtg cagcaggttt ttctactggt atgcttgtaa
     3661 ataatatgaa aattgcagcg caatctagtg gagttgaggc agaaatagag gcgttttctc
     3721 agtctaaatt agcggattat gcgccaaata tagatgttgc actattgggt ccacaagttg
     3781 cttatacatt agataaatca aaagaaattt gtgataagtg tgatgttccg atagctgtta
     3841 ttccgatgat ggactatggt atgttagatg ggaaaaaagt attagatttg gccctatctt
     3901 tgattagtgg gtaagaaaag gagatttatt atgtcaaaga tggatgttca gaaaatcatt
     3961 gcaccgatga tgaagtttgt gaatatgcgt ggcattatag ctctaaaaga tgggatgtta
     4021 gcaattttgc cattgacagt agttggtagt ttgttcttga ttatgggaca attgccgttc
     4081 gaaggattaa ataagagcat tgctagtgtt tttggagcta attggacaga gccgtttatg
     4141 caagtatatt caggaacttt tgctattatg ggtctaattt cttgtttttc aattgcctat
     4201 tcttatgcta agaatagcgg agtagaggct ttaccagctg gagttctatc tgtatctgca
     4261 ttctttattt tgctaagatc atcttatatc cctaaacaag gtgaggcgat tggggacgct
     4321 attagtaaag tttggtttgg aggccaagga attatcggtg ctatcattat aggtttggta
     4381 gtaggaagta tttatacctt ctttataaag agaaaaattg ttattaagat gccagaacaa
     4441 gttccacaag ctattgccaa acagtttgaa gcaatgattc cagcatttgt aattttctta
     4501 tcttctatga ttgtatatat tttagcgaag tcattgacta atggcggaac attcatagaa
     4561 atgatttatt ctgctattca agttccgttg caaggtttaa ctggatcttt gtatggtgct
     4621 attggaattg cattctttat atcatttttg tggtggtttg gtgttcatgg gcaatcggta
     4681 gtaaatggag tagtgacagc tctgctttta tctaatcttg atgctaataa agctatgtta
     4741 gcctctgcta atctatcatt agaaaatggt gcacatattg ttactcaaca atttttagat
     4801 tcatttttaa ttctatcagg ttcagggatt acgtttggtc ttgtagttgc catgcttttt
     4861 gcagcaaaat caaaacaata ccaagcctta ggaaaagttg cagcttttcc agcaatattt
     4921 aacgtaaatg agccagttgt atttggattt ccgattgtca tgaatccagt tatgtttgta
     4981 cctttcattc ttgttcctgt acttgcagct gtgatagtat atggagctat tgcaacaggt
     5041 ttcatgcagc cattctcagg ggtaacattg ccttggagta caccagctat tttatcagga
     5101 tttttggtgg gtggatggca aggagttatt actcagctgg tgatattagc gatgtctaca
     5161 ttggtttatt ttccattctt taaagtacag gatcgtttag cttaccaaaa tgaaatcaaa
     5221 caatcttaga ggtatttgtg tgttactgtt aaactcacac atttgtgcta aaaattagag
     5281 agttaaaatt tttctagtta aaagcttgaa aatttctata aaaatcggta ttatattttc
     5341 gaaagaaata aaaatatttt cgaaagaaag gtgcttacga tggtaaatac agaagtagca
     5401 agaacaacaa tcaagacaga atattttggc agccttactg aaaggatgaa caaatatcga
     5461 gaagatgttt taaataaaaa accttctatt gatgctgaga gagcagttct agcaacacgc
     5521 gcctatgaac gatacaagga acaacctaat gtcctaaaac gtgcatatat gctgaaagaa
     5581 attttggaaa atatgactat ctatattgaa gaagaatcta tgattgcggg aaatcaagct
     5641 tcttccaata aagatgctcc tatttttccg gaatatacgc tagaatttgt tctcaatgag
     5701 ttggatcttt ttgaaaagcg tgatggagat gttttctata ttacagaaga aacaaaagaa
     5761 caacttagaa gtattgctcc gttttgggaa aataataatt tacgtgctag agctggtgcc
     5821 ttattacctg aagaagtgtc tgtttatatg gaaacaggat tcttcggtat ggaaggtaag
     5881 atgaattctg gagatgctca cttagcagtt aactatcaga aacttttgca atttggttta
     5941 agaggttttg aagagcgggc tcgtaaagca aaagtagctc tagatttaac agatccagca
     6001 agtattgata aatatcattt ttacgactct atatttatcg taatcgatgc tattaaagta
     6061 tatgcaaagc gctttgttgc tcttgctaaa agtttagccg aaaatgcaaa tcctaaacgt
     6121 aagaaagaat tacttgagat tgcagatatt tgctctagag tcccatatga accggcaact
     6181 acttttgcag aagctattca atcagtttgg tttattcaat gtattttaca aattgaatct
     6241 aatggccact ctctttcata tggccgtttt gatcaatata tgtatccata tatgaaggct
     6301 gatttagaaa gtggtaaaga aacagaagat agcattgttg aacgtctgac aaatctttgg
     6361 attaagacaa ttacaattaa taaggttcgc agtcaatcac atacattttc ttcagcagga
     6421 agtcctttat atcaaaatgt tacaattggt ggacagactc gagataagaa ggatgctgtt
     6481 aacccattat cttatttggt attaaaatca gttgcacaaa cccatctacc gcaacctaat
     6541 ctaactgtac gttaccatgc aggtttagat gctcgtttca tgaatgagtg tattgaagtg
     6601 atgaaacttg gttttggtat gcctgcattt aataatgatg agattattat tccttctttt
     6661 attgcaaaag gagtattgga agatgatgct tatgattaca gtgccattgg atgtgttgaa
     6721 acggcagttc cagggaaatg gggctatcgt tgcacaggta tgagttatat gaacttccct
     6781 aaggttctac ttatcacgat gaatgatgga attgatccgg cttcgggtaa acggtttgca
     6841 ccaagctttg gtcgttttaa ggatatgaag aacttttctg aattagaaaa tgcttgggat
     6901 aaaacactaa gatatttgac acgaatgagt gttattgttg aaaattctat tgatttatca
     6961 ttggaacgag aagttcctga tattctatgt tcagcattga ctgatgattg tattggtcgt
     7021 ggaaaacacc ttaaagaagg tggagcagta tatgattata tatcaggatt gcaagttgga
     7081 attgcaaatt tgtcggattc attagctgca attaaaaaat tggtgtttga ggaagaacgt
     7141 ataagcccaa gtcagctttg gcatgcactg gaaacagatt atgccggaga agaaggtaag
     7201 gtcattcaag aaatgttgat tcatgatgca cctaagtatg gtaatgatga tgattatgct
     7261 gacaaattgg ttactgctgc ttatgacatt tatgttgatg aaattgctaa atatcctaat
     7321 acacgttatg gaagagggcc tattggagga attcgttatt caggaacatc ttctatctca
     7381 gccaacgtag ggcagggacg tggaacatta gcaactccag atggacgcaa cgcgggtaca
     7441 ccgttagcag agggttgttc accatcacat aatatggatc aacacggccc tacatctgtt
     7501 ttaaaatctg tttcaaaatt accaacagat gaaatcgtag gtggggttct cttaaatcag
     7561 aaagtaaatc ctcaaacgtt agccaaagaa gaagataaat taaaactaat tgctttgtta
     7621 cgaacattct ttaatcgttt acatgggtac catattcaat acaatgttgt ttccagagag
     7681 acgctgattg acgctcagaa acatcctgaa aaacacagag acttaattgt tcgtgttgca
     7741 ggatactctg cattcttcaa tgttctttct aaggcaaccc aagatgacat tataggacgt
     7801 actgagcata ctttgtaaaa taaagaggtt ctttttatgg aatttatgct tgacacatta
     7861 aatttagatg agattaaaaa gtggtctgaa attttgccgc tagctggggt aacttcaaat
     7921 cccactattg caaaaagaga gggttctatt aatttttttg aacgaatcaa agatgtaaga
     7981 gaattgattg gctctacacc ctctattcat gttcaggtga tttctcaaga ttttgaaggc
     8041 atcttaaagg atgctcataa aattcgaaga caagcaggag atgatatatt tatcaaagta
     8101 cctgttactc cagctggatt acgtgcaata aaggcgctaa aaaaagaggg ctaccatatc
     8161 actgcaacag ctatttatac agttattcag ggattattag ctatcgaagc aggagcggat
     8221 tacctagctc catattataa tagaatggaa aatctgaaca ttgattcaaa ttctgtcatt
     8281 cgtcaattag ctcttgctat tgatagacag aactctccta gtaagatttt agctgcatcc
     8341 tttaaaaatg tagcacaagt aaataatgct ttagctgcag gtgcgcatgc tgttacagca
     8401 ggagcggatg tttttgaatc agctttcgcc atgccatcta tccaaaaggc ggttgatgat
     8461 ttttctgacg attggtttgt tattcaaaat agtcgttcca tttagataga gaggaaatac
     8521 atatgagaat ttttgctagt ccttctagat atattcaggg ggaaaatgcc ttgtttgaaa
     8581 atgccaaatc aattttggat ttgggaaatt gccctattct attatgcgat cagttggttt
     8641 atgatattgt tggaaaacga tttgaagatt acctacatag gtatggtttc catattgttc
     8701 tggcgctatt taatggtgaa gcttctgaca atgaaatcaa tcgagttgtt gccttggctg
     8761 agaaagaaaa ttgtgatagt attatcggtc ttggtggggg aaagacgatt gatagcgcaa
     8821 aagctattgc agatttgatt gaaaagcctg ttattattgc tccaacaatt gcatcgaccg
     8881 acgcacctgt atctgcttta tctgttattt atacagatga aggtgcattt gatcattatc
     8941 tattttattc taaaaatcca gatttagttt tggttgatac aaaagttatt tcacaagccc
     9001 ctaagcgttt attagcgtct ggtattgcag atggtttagc aacttgggtt gaggcgcgtg
     9061 cggttatgca ggcaaatgga aaaactatgt tgggacaaca gcaaacattg gctggagttg
     9121 caattgcgaa gaaatgtgaa gaaacgctgt ttgcagatgg tttacaggct atggcagctt
     9181 gtgaagctaa agtggtgaca ccagcattag aaaatattgt tgaagctaat actttattga
     9241 gtggtctagg ttttgaaagt ggaggattag ctgcggcgca tgcaattcat aatggtttta
     9301 ctgcattgac aggtgacatt catcatttaa cacatggtga aaaagtagct tatggaactt
     9361 tagtacaact attattggaa aatagaccta aagaagaact tgataagtat attgagtttt
     9421 acaaaaaaat tggtatgcca acaactctaa aagaaatgca tttggatcaa gttggatatg
     9481 atgatttaat aaaagttggt aaacaagcaa ctatggaggg tgagacaatt catcagatgc
     9541 cgtttaagat ttcgccttca gatgttgctc aagctattat cgctgtagat gcctatgtaa
     9601 attcaaaata aacaataagg actactgttt tccaaatggt agtcttttat tgatccctgt
     9661 attgaattct atagaagatt gaaata
//
LOCUS       DAVKNL010000068         9647 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVKNL010000068 DAVKNL010000000
VERSION     DAVKNL010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9647
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.068"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            48..1529
                     /locus_tag="VW411_001782"
     CDS             48..1529
                     /locus_tag="VW411_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9794468.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSP
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            1891..2892
                     /locus_tag="VW411_001783"
     CDS             1891..2892
                     /locus_tag="VW411_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9794469.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     TVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILEKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
     gene            3044..3961
                     /locus_tag="VW411_001784"
     CDS             3044..3961
                     /locus_tag="VW411_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9794470.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAARLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            3972..4862
                     /locus_tag="VW411_001785"
     CDS             3972..4862
                     /locus_tag="VW411_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9794471.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            4891..6507
                     /locus_tag="VW411_001786"
                     /pseudo
     CDS             4891..6507
                     /locus_tag="VW411_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            6517..7836
                     /locus_tag="VW411_001787"
     CDS             6517..7836
                     /locus_tag="VW411_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU9794472.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEETGVRQVQNMVFSDDGIHFEKISQNPVATGSDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWREKRFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDIDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            8234..8647
                     /locus_tag="VW411_001788"
     CDS             8234..8647
                     /locus_tag="VW411_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794473.1"
                     /translation="MKNNERLGIKLSRDSVLGLREVRRLYLGSSDIPVSDGYVIEVAY
                     NQISHEIDIIDWVELNKSKIKISEISESVDIDATSLRTTLTLDTLVYEGMRDIQLKLR
                     ELTKGRVFFSFVVKLVLFASILKKKDLLEKFQEKC"
     gene            8898..9473
                     /locus_tag="VW411_001789"
     CDS             8898..9473
                     /locus_tag="VW411_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794474.1"
                     /translation="MKQKQPIVSRTKQHTFEELIQDQKLERLAKLSPDLVGRYGFTAS
                     CASSFANLIKEAYGGKNLNVVYASRMLALWNIACSCYHKADGYSLADALFSDKKICLD
                     SYYYHKNTSNTITSDVIKDVYDNYNNYMVLTREATPEYIYVVQTEMPKDSDLYFYIRE
                     VLGLSFSTMHYAFLVKVLAGALARKYKPYRN"
BASE COUNT         3224 a         1470 c         1856 g         3097 t
ORIGIN      
        1 attgcaagag gtttaatccg ataatttata aagagagaaa gatattcatg agagatttat
       61 tatctaaaaa aagtcatagg caattagaat tattagaatt attatttgaa cataaacgtt
      121 ggtttcatcg ttctgaacta gcagagttat taaattgtac agaacgtgca gtcaaagatg
      181 atctatccca tgttaaatct gcttttcctg acttgatttt tcattcttct actaatggta
      241 tacgcattat taataccgat gatagtgata ttgaaatggt ttaccatcat ttctttaaac
      301 attcaactca tttttcgatt ttagaattca tcttctttaa tgaaggttgt caagctgaga
      361 gtatttgtaa agaattttat atcagttcat cttcgctcta tcgtattatt agccaaatca
      421 ataaagtgat taaaaggcaa tttcaatttg aagttagtct gacccctgtt caaatcattg
      481 gaaatgagag agacattcgt tacttttttg cacaatattt ttcagaaaaa tattatttcc
      541 tagaatggcc atttgaaaat ttttcatcag agccactatc tcaattgtta gaattggttt
      601 ataaggaaac aagctttcca atgaatttgt caacgcatag aatgctaaag ttgctcctag
      661 ttacgaacct atatagaata aagtttggtc attttatgga agtagataaa gattctttta
      721 acgaccaaag tttggatttt ttaatgcagg cagaaggaat agaaggtgtt gctcagagtt
      781 ttgaatcaga atacaatatc tctttagatg aagaagttgt ttgccaatta tttgtgtctt
      841 actttcaaaa aatgtttttc atagatgaaa gtctctttat gaaatgcgta aaaaaggata
      901 gttatgttga aaaatcttac catctattga gtgattttat tgatcagatt tcagtcaagt
      961 atcagattga aatggagaat aaggataatc tgatttggca tctgcataat accgcacatc
     1021 tgtatcgtca agagttgttt actgagttta ttttatttga tcaaaaaggg aatacaatca
     1081 ggaactttca aaatattttt ccaaaatttg tttcagatat aaaaaaagag ctttctcact
     1141 atttagagac tcttgaggtt tgttctagtt caatgatggt aaatcatcta tcttatacct
     1201 tcatcactca taccaaacat ttggtgatta atttgctaca aaatcagcct aaactaaagg
     1261 ttttagttat gagtaatttt gatcagtatc atgcaaaatt cgttgcagag acactttctt
     1321 attattgtag caataatttt gaactggaag tttggactga attagaatta tcaaaggaat
     1381 ctttagaaga ttcaccttat gatatcatta tttctaattt tattattcct cctattgaaa
     1441 ataagagact catctattca aataatataa acacggtctc actcatatac ttgttaaatg
     1501 ccatgatgtt tattcgatta gatgagtaac caaaaataac aaaaaaacgc tatttcgcgt
     1561 ttgaactgta cctcaaaagt tagacagaaa aagtctaatt ttgggggtgt ttttgttaag
     1621 aaaagacctt caatcattta agcaaaatgt acatctagat ttatagccat ctaaaactct
     1681 tgcttttttt atagaagata cgaaaaaagc catgaaacca aggcttttcc tgttttatgt
     1741 agatgtttac acatgaaaat tgaagtattt tcaaaagcta attggagtga ttttattcat
     1801 gatgggataa atgatctggc caaaaatgat tgagaaatag ttgcttaaaa agcatttgca
     1861 tgatactata tattgaaagg aggtgataat atggtagaac aaagaaaatc aattaccatg
     1921 aaagatgttg ctttagaagc aggagttagt gttggaactg tttcacgtgt aattaataaa
     1981 gaaaaaggca ttaaagaagt aactttgaaa aaagtggaac aagcgattaa aactttgaat
     2041 tacattccag attactacgc tagaggaatg aaaaaaaatc gaacagaaac gattgcaatc
     2101 attgtaccaa gtatctggca tcccttcttt tcagaatttg ctatgcatgt ggaaaatgaa
     2161 gtctataaga gaaataacaa attactctta tgttctatca atggtacaaa tagagagcaa
     2221 gactatctgg agatgttgcg tcataataaa gttgatggag tggttgccat tacctatagt
     2281 ccaattgaac attacttgac gtcaggaatt ccctttgtta gtattgaccg cacatactca
     2341 gatattgcca ttccttgtgt ttcatcagat aatgatgcag gtggaagaga agcagctaaa
     2401 caattaatta gtaaaggatg tcagcattta gcctttgtgg gcggacataa tacaaccatt
     2461 aatgaaacca aacgtcgtag aatttcattt gaaaagtatg tccaagagca taaaatacct
     2521 cttagtattt ttgatttgga tgagacagtt actgactatt atggaaagct agagagtttc
     2581 ttggaagaaa attcttctat agatggaatt tttacaatca acgattttac agctttggat
     2641 gtgattgaaa tactagaaaa aaatggcaaa cgtattcctc atgatgtcca aattattgga
     2701 tacgatggaa ttaaaatggc tggagataga gattatctac tttcaactat tgaacaacca
     2761 ttggaagaaa tggcaaaaga agcggttcgt attttgtttg atataattga tggaaagact
     2821 gttaatttgc agacaatcct accagtaaaa tttgttgaag gaaaaacaac aaaaaatgaa
     2881 aataagtctt gacagaaagc gctatcaatg atagaatgaa ttcagataaa aagatttatt
     2941 tttaaaacaa aaaatgaaac gtttcaaaaa aagaaataaa gagacagcgc caagcgctat
     3001 cttttctaga aaaaaatgaa acgtttcaaa aaaggaggtt gctatgaata gcaaagcgaa
     3061 gcaagtttct ctttgggaaa gaatcaagaa acaaaaactc ttgttattga tgactgtccc
     3121 cggtttagtt ttaaccttta tctttaaata catccctatg tatggggttt taatcgcatt
     3181 taaagattac aatcctttaa aaggaatttt agggagtgat tggattggtt tttctgagtt
     3241 tacaaaattc atatcctctc ccaactttgg tatcttgtta gccaacacat taaaattaag
     3301 tatctatggt ttattgcttg gctttttacc accaatcatt ctcgcgatta tgctcaatca
     3361 actcttgagt gaaaaagtca aaaaacgaat tcagctcatt ttatacgcac caaactttat
     3421 ctcagtcgtt gttattgtcg gtatgatttt cctcttcttt tcagtgggag gaccaatcaa
     3481 caattttctt tctatgtttg gaatgaaggc tgacttcttg acaaatccag acttctttag
     3541 acctttatac atctttagtg gtatctggca aggaatgggc tgggcttcaa cgctctacac
     3601 ggcaacattg gtaaatgtag atccagcctt agtagaagca gcccgactgg atggagccaa
     3661 tatcttccaa cgaatctggc acattgatat tccagctctt aagcctatta tggttatcca
     3721 atttgtttta gctgcaggtg gaattatgaa tgtcggatat gaaaaagcat tcttgatgca
     3781 gacatcgtta aatttgccaa cttctgaaat tatctcgaca tatgtctata aagttggtct
     3841 tgtatcagga gactattctt actcaacagc ggttggtttg tttaatgcag tgattaacgt
     3901 agtattgctt gttgcagtta accaaatcgt taaacgcatg aataatggtg aaggaattta
     3961 aggaggaaag tatgaaaaat tcgattatgg atacaaaatt tgatagacgt atcttactct
     4021 taaataaaat cattattgtc tttatcgttt tgatgacttt gcttccttta ctttatatcg
     4081 tcgtagcatc ctttatggat cctaaggttc tggttagtag agggattagc tttaatccag
     4141 ccgattggac tgtagaaggt taccagcgtg tattcagtga ccaatctatt ctaagaggtt
     4201 ttatcaattc tctactatac tcttttggat ttgcagcttt aacagtcttg ctatctgtgt
     4261 ttacagctta tcctctttct aagaaagact tggttggacg tcgttggatt aactacttct
     4321 tgattgtaac tatgttcttt ggtggtggtt tagtcccaac ttacttgctc gtaaaagaat
     4381 tgggaatgct caatactcca tgggctatca ttgttccagg tgctgttaac gtttggaata
     4441 ttattcttgc tagggcctat ttccaaggat tgcctgaaga attagttgaa gctgctgtca
     4501 ttgatggtgc aaatgattta cagattttct tcaaaatcat gcttcctctt gcaaaaccaa
     4561 ttatgtttgt tctcttcctt tatgcttttg taggacagtg gaactcatac tttgatgcaa
     4621 tgatttatat caaggatcca aacttggaac cattgcaact tgtacttcgt aaaattctca
     4681 ttcagagcca accaggtcaa gacatgattg gagcacaagc ggctatgaat gaaatgaaac
     4741 gtttagctga attgattaaa tacgcaacta ttgtcatttc cagcttgcca ttgattgtta
     4801 tgtatccatt cttccaaaaa tactttgata aaggaattat ggctggttca cttaaaggat
     4861 aaaaaaagaa aaaataaaag gagttttctc atgaaattca aaacattctc aaaatcagca
     4921 gttttgttga cagctagttt agcagtactt gcagcctgtg gctcaaaaaa tacagcttca
     4981 agtccagatt ataagttgga aggtgtaaca ttcccgcttc aagaaaagaa aacattgaag
     5041 tttatgacag ccagttcacc gttatctcct aaagacccaa atgaaaagtt aattttgcaa
     5101 cgtttggaga aggaaactgg cgttcatatt gactggacca actaccaatc cgactttgca
     5161 gaaaaacgta acttggatat ttctagtggt gatttaccag atgctatcca caacgacgga
     5221 gcttcagatg tggacttgat gaactgggct aaaaaaggtg ttattattcc agttgaagat
     5281 ttgattgata aatacatgcc aaatcttaag aaaattttgg atgagaaacc agagtacaag
     5341 gccttgatga cagcacctga tgggcacatt tactcatttc catggattga agagcttgga
     5401 gatggtaaag agtctattca cagtgtcaac gatatggctt ggattaacaa agattggctt
     5461 aagaaacttg gtcttgaaat gccaaaaact actgatgatt tgattaaagt cctagaagct
     5521 ttcaaaaacg gggatccaaa tggaaatgga gaggctgatg aaattccatt ttcatttatt
     5581 agtggtaacg gaaacgaaga ttttaaattc ctatttgctg catttggtat aggggataac
     5641 gatgatcatt tagtagtagg aaatgatggc aaagttgact tcacagcaga taacgataac
     5701 tataaagaag gtgtcaaatt tatccgtcaa ttgcaagaaa aaggcctgat tgataaagaa
     5761 gctttcgaac atgattggaa tagttacatt gctaaaggtc atgatcagaa atttggtgtt
     5821 tactttacat gggataagaa taatgttact ggaagtaacg aaagttatga tgttttacca
     5881 gtacttgctg gaccaagtgg tcaaaaacac gtagctcgta caaacggtat gggatttgca
     5941 cgtgacaaga tggttattac cagtgtaaac aaaaacctag aattgacagc taaatggatt
     6001 gatgcacaat acgctccact ccaatctgtg caaaataact ggggaactta cggagatgac
     6061 aaacaataaa acatctttga attggatcaa gcgtcaaata gtctaaaaca cttaccacta
     6121 aacggaactg caccagcaga acttcgtcaa aagactgaag taggaggacc actagctatc
     6181 ctagattcat actatggtaa agtaacaacc atgcctgatg atgccaaatg gcgtttggat
     6241 cttatcaaag aatattatgt tccttacatg agcaatgtca ataactatcc aagagtcttt
     6301 atgacacagg aagatttgga caagattgcc catatcgaag cagatatgaa tgactatatc
     6361 taccgtaaac gtgctgaatg gattgtaaat ggcaatattg atactgagtg ggatgattac
     6421 aagaaagaac ttgaaaaata cggactttct gattacctcg ctattaaaca aaaatactac
     6481 gaccaatacc aagcaaacaa aaactagagg ttgattatgg gagataagaa atacacagta
     6541 gaaaaagcca atcgttttat agcagaaaat aaacatctcg ttaatactca atataagcct
     6601 gaagaacatt tttcagctga gattggttgg atcaatgatc caaatggatt tgtctatttt
     6661 cgtggagaat accatctctt ttatcaattc tatccatatg atagtgtttg ggggcctatg
     6721 cactggggac atgctaaaag taaggacttg gtgacttggg agcacttgcc agtggcactt
     6781 gctcctgacc aagattatga ccgaaatggt tgtttctcag gctctgccat tgtcaaggat
     6841 gatcgcctct ggctcatgta cactggacat atcgaagaag aaaccggtgt ccgccaagtg
     6901 caaaatatgg tattttcaga tgacgggatt cactttgaaa agatttccca aaatccagtt
     6961 gcaactggat cagacttacc agatgagttg attgctgctg atttccgtga tccaaaactc
     7021 tttgaaaaag atggacgcta ttactccgta gtagctgcca aacacaagga taatgtgggc
     7081 tgtatcgttc tactagggtc cgataaccta gtagaatggc agttcgaatc catcttttta
     7141 aaagggggag aacaccaagg ttttatgtgg gaatgcccag attacttcga gttagatggg
     7201 aaagattgcc ttattatgtc acccatgcgt tatcagcgtg agggagactc atatcataac
     7261 atcaactcat cgcttttgtt cacgggtaag gtagattgga gagaaaaacg ttttatccca
     7321 gaatcagttc aagaaattga tcatggccaa gacttctatg cgcctcaaac attgttggac
     7381 gatcaaaatc gtcgtatcct gattgcttgg atgcagacat gggggcgtac ccttccaacc
     7441 catgaccaag aacacaagtg ggcatgtgcc atgactctac ctagaattct aagattggaa
     7501 gatggcaaac taagacaatt ccctgttaaa aaaggccaat atcaaatcca aatagataaa
     7561 gattgtcatt accacttagg aaatgatata gattatcttg aatttggtta tgacagtaat
     7621 gcgcagcaag tttacattga tcgtagccat cttattcaaa aaattctagg tgaagaagaa
     7681 caggacacta gtcgacggta tgtagatatt gaagctaaag aattggaagt tgttctagat
     7741 aaaaattcca tcgagatttt tgtcaatcaa ggtgaagcaa gcttgactgc aacttattac
     7801 ttaacggtgc cagctgagct atcacgaatt gattaaaaat taagttattt ctcctaaaga
     7861 aaaagttctc tttctaaaat agtggaaaga ggactttttg tgttttgggt atataagctt
     7921 agtttatggt atttgtaaaa ttggtgttgg attatgattt aagctagttt tctaaagaat
     7981 ttgaaaaaaa ttttatttaa gcaaaaaaac cttggttcca aggcttttcc tgttgtattt
     8041 agatgccccc tacagggatt gtaggagata tgttgcttag atgttcttga ttttctggtg
     8101 ttttgtaacg tttaaatgag ttttttgagt ttgttggtgg ggcgttgccc ggcaattgcc
     8161 cgacttattg cttgaaaaag aatttaaaat atagtatagt taattataga ttaacacttg
     8221 cttggaggaa ctgatgaaga acaatgaaag attaggtatt aaattaagta gagatagcgt
     8281 tttaggattg agggaagtta gaaggcttta tttaggcagt tcagatatcc cagtttctga
     8341 tggctatgtg attgaagttg cttataacca gatatcacat gagattgata ttattgattg
     8401 ggtagagttg aacaagtcaa aaattaagat aagtgaaatt agtgaaagcg tggatataga
     8461 tgccactagc ttgagaacaa ctttgacttt agacacatta gtatatgaag gtatgagaga
     8521 tatacagtta aagttgagag agcttacaaa ggggagagta ttcttttcat ttgtagtgaa
     8581 gttagttttg tttgcttcta ttttaaagaa aaaagattta ctagaaaaat ttcaagaaaa
     8641 gtgttaatca agtattgaca ctttatctgg atttcggtat aatatgctta gaaaggaatc
     8701 tttctaaatt tttttcgtcc ttatgtgtta atcaaagacg aatacaaaaa catatttttt
     8761 tactctaaaa agtgttaatc aatgatgtat ttgttagaga ggtagataaa tggaattgag
     8821 agcaccacca gttataatag tataaaacgt ataataaaaa tattttaact tgaattatag
     8881 aaaaggagaa acaaatcatg aaacaaaaac aaccgattgt ttctagaacg aaacaacata
     8941 catttgaaga gcttattcaa gaccaaaagt tagaaagatt ggctaagttg tcgcccgatt
     9001 tggttggaag gtatggtttt actgctagct gtgcgtcttc atttgcgaac ttgattaaag
     9061 aagcgtatgg gggtaaaaat ctaaacgtag tttatgcgag tcggatgttg gctctctgga
     9121 atattgcttg cagttgttat cataaggctg atgggtattc tttagcagat gcgcttttta
     9181 gtgataaaaa aatttgtcta gattcttact attaccacaa gaatacctct aataccataa
     9241 ctagtgatgt gataaaagat gtttacgata attataataa ttatatggtt ttaactcgag
     9301 aagcgacacc tgaatacatt tatgttgtac aaactgaaat gccaaaagat tcagatttat
     9361 atttttatat tagagaagtt ctgggattat cgtttagtac catgcattat gcatttttag
     9421 tcaaggttct tgcaggagcg cttgctagaa aatataagcc atatcgaaat tgaattattt
     9481 aaatttatac tcttcgaaaa tcaaattcaa accacgtcag cttcgccttg ctgtactcaa
     9541 gtgctgtctg tggctagctt cttagtttgc tttttgattt tcattgagta ttactcttat
     9601 ggtagttatt tatggcataa taatattgat ttgggagtta tagcgaa
//
LOCUS       DAVKNL010000069         9615 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVKNL010000069 DAVKNL010000000
VERSION     DAVKNL010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9615
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.069"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            <1..765
                     /locus_tag="VW411_001790"
     CDS             <1..765
                     /locus_tag="VW411_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU9794475.1"
                     /translation="KMSFIAQDFNNLNIITVLKGRTQAVIRNHFLKYDRAVRCRVKII
                     TMDMFSPYYDLARQLFPCAKIVLDRFHIVQHLSRAMSRVRVQIMNQFHRKSHEYKAIK
                     RYWKLIQQDSRKLSDKRFYRPTFRMHLTNKEILNKLLSYSQNLKHHYQLYQLLLFHFQ
                     NKEPEKFFGLIEDNLKQVHPIFQTVFKTFLKDKKKIVNTLQLPYSNAKLEATNNLIKL
                     IKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
     gene            complement(816..1751)
                     /gene="dapA"
                     /locus_tag="VW411_001791"
     CDS             complement(816..1751)
                     /gene="dapA"
                     /locus_tag="VW411_001791"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEU9794476.1"
                     /translation="MSYQDLKKCKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKTIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(1807..2883)
                     /locus_tag="VW411_001792"
     CDS             complement(1807..2883)
                     /locus_tag="VW411_001792"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEU9794477.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGSSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(3225..3297)
                     /locus_tag="VW411_001793"
     tRNA            complement(3225..3297)
                     /locus_tag="VW411_001793"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3262..3264),aa:Thr,seq:ggt)
     gene            complement(3397..3906)
                     /locus_tag="VW411_001794"
     CDS             complement(3397..3906)
                     /locus_tag="VW411_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEU9794478.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(3904..4000)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            4029..4460
                     /locus_tag="VW411_001795"
     CDS             4029..4460
                     /locus_tag="VW411_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEU9794479.1"
                     /translation="MKKPIQKFFNNEILAYLFFGIVTTLVAILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            4542..4919
                     /gene="mscL"
                     /locus_tag="VW411_001796"
     CDS             4542..4919
                     /gene="mscL"
                     /locus_tag="VW411_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEU9794480.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKEE
                     KTDEKKPTELEVLQEIKALLEKK"
     gene            complement(4961..6055)
                     /gene="hemH"
                     /locus_tag="VW411_001797"
     CDS             complement(4961..6055)
                     /gene="hemH"
                     /locus_tag="VW411_001797"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEU9794481.1"
                     /translation="MKKAILMMTFGSPEEIIFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGSEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESQDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            6210..7433
                     /gene="pepT"
                     /locus_tag="VW411_001798"
     CDS             6210..7433
                     /gene="pepT"
                     /locus_tag="VW411_001798"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEU9794482.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEA
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(7469..8098)
                     /locus_tag="VW411_001799"
     CDS             complement(7469..8098)
                     /locus_tag="VW411_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794483.1"
                     /translation="MSKANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDPQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSAKSKDKTALLESTKAILVLNHQIQSKASASEETSPARNAEANGDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            8063..8284
                     /locus_tag="VW411_001800"
     CDS             8063..8284
                     /locus_tag="VW411_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794484.1"
                     /translation="MDLFQELICLAQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(8555..9566)
                     /locus_tag="VW411_001801"
                     /pseudo
     CDS             complement(8555..9566)
                     /locus_tag="VW411_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
BASE COUNT         2895 a         1956 c         1720 g         3044 t
ORIGIN      
        1 aagatgagct tcattgcgca agattttaac aatctcaata tcatcactgt tcttaaggga
       61 agaacacaag ctgtcatccg aaatcacttt cttaaatatg atagagccgt ccgatgtcgc
      121 gtcaaaatca ttactatgga tatgtttagt ccttactatg acttagctag acaacttttc
      181 ccgtgtgcta aaatcgtgtt ggatcgcttt cacattgtac aacatcttag ccgtgctatg
      241 agtcgtgtgc gtgtccaaat catgaatcag tttcatcgaa aatcccatga atacaaggct
      301 atcaagcgct actggaaact cattcaacag gatagccgta aactgagtga taagcgattt
      361 tatcgtccta cttttcgtat gcacttaacc aataaagaga ttttaaacaa gcttttgagc
      421 tattcacaaa acttgaaaca tcactatcag ctctatcaac tcttgctgtt tcactttcag
      481 aataaggaac cggagaaatt tttcggactc attgaggaca atctgaagca ggttcatcct
      541 atttttcaga ctgtctttaa aacctttcta aaggacaaaa agaaaatcgt caacaccctt
      601 caactaccct attcaaacgc caaattggaa gcgaccaata atctcatcaa acttatcaag
      661 cgcaatgcct ttggttttcg gaactttgaa aacttcaaaa aacggatttt tatcgctctg
      721 aatatcaaaa aagaaaggac aaaatttgtc ctttctcgag cttagctgac ttcaacccac
      781 tacagttgac aaagagccgg attttattgt ctttattagt aatctggtct taagacccct
      841 gttacagttg ccttagttgc ttcgtagtcg ccatctacga caaccttgat aatgcgtttg
      901 gcatcttctt ctggtgctgg aacaagaggt agacgagtgg gtccagcttc aaatcccata
      961 tagttaagaa ttgccttaac tggagcagga cttggataag agaagagagc attaacctta
     1021 ggaatgaatt tacgctgaat tgctgcggct ttcttcatat cgctttctgc aatcgcagta
     1081 aacatctcgt gcatttcatc cccatttgta tgagaggcaa cagaaataac cccatccgcc
     1141 ccaaggttca tggcatggaa agcatctcca tcctcacctg tataaatcaa gaactcttca
     1201 ggcttgtgct caatcaagta agccatatta gccaagctag tacattcttt gacaccgata
     1261 atatttggat ggtcagccaa gcgaagcatg gtttctggag tcaattcgac aactacacgc
     1321 cctggaatgt tatagataat aattggtagg tcagaagcat ctgcaatagt cttaaagtgc
     1381 tgatacatcc cttcttgaga aggtttgttg tagtaaggaa caatagcaag cccagctgcg
     1441 aaaccaccaa attccgctac ttctttgaca aactcaatag agtcacgcgt atcattagta
     1501 cctacacccg caatcaaagg aacgcgtcca ttgacaacct tttgtacagc cgcaaacaac
     1561 tccaactcct catcgtgggt caaagttgga ctctcagcag tcgttcctgc gagaagaatt
     1621 ccatccgtat gatgggccaa taaatgctca atcaaggctg gaatagcatc aaagttaatg
     1681 gaaccatcct catggaaggg ggtaataaag gctgtaatga ttttacattt ttttaaatct
     1741 tgataagaca cgaaaacacc tctctatttc aaagaaggag ttcatctgaa ctcctaaacg
     1801 atatgactat tttaattcaa atttcaattc ggctgttgga cgaaccaatc cacgttcatg
     1861 aagagtttca gcaatctgaa ctgagttcca agcagcacct ttgagaaggt tatctgaaac
     1921 aacccacatg tgaattcctt tttctgcatc caagtcttta cggatacgac caacaaaggt
     1981 atcacgcgaa ccaactgcat tgatagcttg aggatagatt tgatgagcta catcatcttc
     2041 aagaacagca cctgggaagg ctgcgatagc tgcttttact tcttcgattg gagccacttc
     2101 ttttgtttcg atataaacag actcagagtg agctgacaag actggaatac gcacacatgt
     2161 tgcagatact gcaatgctat catcttccat aattttctta gtttccttgg tcatcttcat
     2221 ctcttcgtac gtgtaatcat tatcagtgaa aacatcaatt tgtggaagag cgttaaaggc
     2281 gataggataa tgtttcttgt caccacctga aggcaagatt tccgcatgca aatcacgtgg
     2341 tttcacacca tcattcaaga cttcacgaag ttcacgttgt gtctcaagaa ttgctcccat
     2401 accagcacct gaaacggctt gataagttga aacaatgata cggtccaagc cccatttttg
     2461 gcgaaccggc tcaagagcca ccatcatttg aattgttgaa caattagggc aggcaatgat
     2521 tccgttgtga gcatcaagtg catgagcatt gacctctgga acaaccaaag gaacatctgg
     2581 attttgacgg aaataagatg tattatctac tactaccacg ccagctttta ctgcgtatgg
     2641 tgcatactta gctgatgtag aactacctgc tgaaaagaga gcaatatcaa ctccttcaaa
     2701 agctgtttca gtcgtttctt caattgtaat atcttgatct ttaaatttca atgacttacc
     2761 tgctgaacgt gcagaagcaa ggtaacgaat tttatcgatt ggaagtgttg attcttccaa
     2821 catttttatc atctgagcac cgacagcacc tgtcgcgccg actacagcaa ctgtatatcc
     2881 cataaataac ctctttcgga attttctaaa aatttctata atagaagtat tatactactt
     2941 tttccatgaa ttgcaaagct ttttcatcat tttttggaaa ataaaaatcc cagtcgctag
     3001 actagggact aagaggcatc ttcatgtgat gagcaggttc acacaactca tcaaggtccg
     3061 ctcctgcgtt atgacctcct catgctcaat agtagtccga agactaccta tcgactcatc
     3121 gcatattcta tattactaga gatacttgat tttgtcaata agaaattttt cagatcgagt
     3181 ataacaaaaa actctcgtat tcaagagttt ccctatacat atcatgctaa ttgccgggat
     3241 tgaaccggcg acctcatcct taccatggat gcgctctgcc aactgagcta aatcagctta
     3301 cttaagaagt atactatatt cagaaaaact tgtcaagttt ccttagaaat tttccatagg
     3361 aaaaagccag gtaaaaacta cctagcttat cttcttctat ttacttaaat caattcgacg
     3421 accaatttta ccaataataa tcgctccaat aatcaatgaa gcaaattcac cgattcctgt
     3481 tgtaaaccaa gtaaggaaga atggtaattc tgctacaata tttaactctg cagcgatagt
     3541 aaacatggaa attgaaaaga ggattgaaaa gaagaaatga tcttttcgaa tcaatccgtt
     3601 aaagaggtaa tccttgctat attttgcaaa aagccaaaca cctaaactga ggaatactaa
     3661 ggttgaacca ccaccaacaa agacatctag cagtccgaag ctaaagaaat tagcaatcat
     3721 acaaccgatg gtaactccga tgatgtactt aggattgtaa aaagccataa agttcatcat
     3781 ttctgaaata cgaaactgat aagcaccata gctgatggca tttaggggcg gtgtgacagt
     3841 caaaacgacg tagatagcag cgacgattgc aatatctgca acatcacgaa tagttaattt
     3901 tttcatcttt tctcctttgg cggttatccg cgtgtaatat gcttggtgaa agaagctaag
     3961 caccaagggt tgattcattc aaccttacta gtatagcaca atagcctgct ttatgctata
     4021 cttaaagcat gaaaaaacct attcaaaaat tttttaacaa tgaaatctta gcctatctct
     4081 tttttggtat agtgactact cttgttgcta tactatcacg attagtcatt tatcagctaa
     4141 gtcatcagga actccttgct acggccttgg caaatattat cggtatccta tttgccttta
     4201 tcacaaatga tacgattgta tttaagcaaa aaaagaaaaa tagacttatc cgtttagtga
     4261 aattttctct tgcccgcctt tctacttttc tgttagactt gtttttaact tttctctttg
     4321 taacccaatt tcctcacatc atagggcaat tcgtaactga aaacattgat aagataaatg
     4381 ctattgaaac aataattgca caaatattca ttattatcat caattatatc ttgagcaagg
     4441 tctatatttt tagaaaataa tgctccattt atgagccttt taattgcctt ctataatgat
     4501 ttaggataca atgaaacacg aaacattgaa aggaaaacag tatgttaaaa aatctaaaat
     4561 cgttcttgct tcgaggaaat gttattgacc ttgctgtcgg tgttgtaatt gcctctgctt
     4621 ttggtgctat cgttacttca cttgtaaacg acattatcac tcctcttatt ttaaatcccg
     4681 ctttgaaagc tgctaaagtt gaacgtatcg ctcaactttc ttggcatgga gtcggctatg
     4741 gtaacttctt aagtgctatt atcaatttta tctttgtggg taccgccctc ttctttatta
     4801 tcaagggcat tgaaaaagca cagaagctga ctggcataaa ggaagaaaaa actgacgaaa
     4861 aaaaaccaac cgaattggaa gtccttcaag aaataaaagc tctccttgag aaaaaataat
     4921 cgattaaaag gatctagcgc aaagctaaat ccttttttct ttactctttg ggtaacttgc
     4981 ctgctttttc aagcattttt ttgataagat aaggcatctt aacattttca cgaccttttt
     5041 tctcaatcaa ttttttcaca aattctggca tttgtaaatc ttgagattca gccaaaatgt
     5101 ttttcgtctc gtctgaagga actaactcat caaaggtctc ttcttctggg agaaattcct
     5161 taaactcttg attttcattg actctaactg tattgaccaa agcatcaatc aaacgagaat
     5221 ctgtatttgg cattggtgga cgatggtagt ttaccccaaa ttcctgacat aagtcataac
     5281 attccacatc attgtcaaac aagacctcaa tatgctcact gatgaaacta ataggtacaa
     5341 aaatataatg gtctggatgt tctgtctgtt ctctgagata ctccaaaaca tctggtttaa
     5401 tccatggaat accaatatca ctctcacttt gccaagtgtt ggtatattgt tctgaactca
     5461 agcccagttt ttcagcgact agcttgctat tttcgaaaat ttggtctata taaggatcac
     5521 caaagtccaa ggcaaaaata ggcacactat gggctgaaaa gatgacttta aagctgtctt
     5581 gctttacttc ttcttttaaa atcttagcaa tttcatctgc ccaatagttt aagagcgctt
     5641 cttcctgata ccagtcctta atgaccaaaa attgaatttg tttgctttct aaaaattttt
     5701 catagcccat gacagagtag aaagaataat ggggctccaa aatcaagcaa atacactgtt
     5761 caattccgtc tgcttccatt tgaccgatca catctggaat aaagggactg gaaaacttat
     5821 tagcaaagta gacactgtat tcactcccca gcctagcttc taccaaggca acctcttgac
     5881 gggtaatttt ttgcagaggc gtgcctccaa ttcgtacata attatcataa agagtctgaa
     5941 tctcgtggtc ttggggtctc accccacgac gaatatttgt gaaaaaatca gctacacctt
     6001 caaagataat ctcttctggt gaaccgaagg tcatcattaa aattgctttt ttcatgactg
     6061 cttccttgtg atgtttttat caagtttatt ttatcattaa ttcatcgata agtaaacgct
     6121 agcataggga cattctcaat cttctgtctt ttgtttttct cttctttcta tgatacaatg
     6181 gaaaaaataa attcaaaagg agttttttta tgacttatcc aaatctcttg gaccgcttct
     6241 taacctatgt taaggtcaac acgcgctctg atgaacactc tactactact ccaagtacac
     6301 agagtcaggt tgacttcgca acaaatgtcc taattcctga aatgaaacgt gttggactgc
     6361 aaaatgttta ctatctaccg aatggttttg ctattggaac cttgccagcc aacgatccgt
     6421 ctttaacacg taagattggt tttatatcgc acatggatac tgctgatttt aatgctgaag
     6481 gagtcaatcc acaggtaatt gaaaactacg atggtggtgt gattgaacta gggaattctg
     6541 gtttcaaact cgatccagct gacttcaaga gtcttgaaaa atatccagga caaacgctca
     6601 tcacaacaga tggaacaacc ttgctaggtg ctgatgacaa gtcaggaatt gctgaaatta
     6661 tgacagccat tgaatatcta actgctcatc ctgaaattaa gcactgtgag attcgtgttg
     6721 gttttggtcc agatgaagaa atcggtgttg gtgccaataa atttgatgca gaagattttg
     6781 atgtggattt tgcctacact gttgatggtg gtccactagg tgaacttcag tacgagactt
     6841 tctcagccgc tggtgctgaa ttgcatttcc aaggtcgtaa tgtccaccct ggtactgcca
     6901 aagggcagat ggtcaatgcc cttcagctag caattgattt tcataatcaa cttccagaaa
     6961 atgaccgacc tgagttaact gaaggttacc aaggttttta ccatctaatg gatgtgacag
     7021 gtagtgttga ggaggcgcgt gcaagctaca tcattcgtga ttttgaaaaa gatgcctttg
     7081 aagcgcgtaa agcatccatg caatctatcg ctgataagat gaatgaagaa cttgggagcg
     7141 accgtgtcac tctcaacttg acagaccagt actacaatat gaaagaagtc attgaaaaag
     7201 atatgactcc aattaccatt gctaaagccg ttatggaaga tctaggtatc acgcctatta
     7261 tcgaaccaat ccggggtgga acagacggct ctaagatttc ctttatggga atcccaactc
     7321 cgaatatctt tgcaggtggc gaaaatatgc acggacgttt tgaatacgtt agccttcaga
     7381 ctatggaacg tgcagttgat accatcattg gcatcgtagc ttataaaggc taaaaagacg
     7441 aggtagctca gctacttcgc ctttcttttt attctactgg tttttcttga tttccagtag
     7501 ttgtagaaga ttctgttgtt tcattttctg aagttgattc agcaggttta gaatctcttg
     7561 tattgcttgg tttgttttcg tcgctagcag tttcaatgtt agattctgca gttgcgtttg
     7621 gttggttctc agcactggtg ttatcaccat ttgcttcagc atttcttgct ggacttgttt
     7681 cttcacttgc gctagctttt gactggattt gatgattcaa aactagaata gcttttgtcg
     7741 attcaagtaa agctgttttg tctttactct tagcagaaag ttgatctaat aatgcatcca
     7801 ccttatcaaa gtccgcatca gatccattat tactttctaa ataagagtga agcgacatga
     7861 gaatatcgta gagtttttga tagagtacaa gtgtctgagg atcttgctca gcattttcct
     7921 tttcttgttg aagggcgcta gcgatacgag tcaagacatc ttttacctga ctgtttactt
     7981 catccaagtc tgcatcagcc ttgtttgtgg cagcttttag attttctact tcttctgcca
     8041 aggattgtct gattccttct tcatggattt gttccaagag ttgatttgcc ttgctcaaaa
     8101 gactttctac ttcttccttg ctatctgtcg cagattattg gttgctatct accatgtact
     8161 cctaaaacag gagagttata atccaagatt acaaggcctt acagaaataa gaaatccaga
     8221 taagacaatg ttcgtccaag acgctattcg cttcgcacag cagcacggat tcaatatgct
     8281 ttaattttaa agtttaggtg tcaagacctc tttttagtgt gcccaaaatt tagagaagta
     8341 atcaatcaac taacttttat ttttttcaaa ctttcagtaa actgacctaa agctaactca
     8401 atctgtcttt gtagatgctt ctgctatcag ctagaagttg atctactttt gccaagactg
     8461 ccttctcatc aaaagttcca ggttgatagt tggattgcag ggatggaatc ttgtttttca
     8521 aagccgcttc atatccctta gtttgaacct tgatgtagtg attgtggtcg ccatgaggaa
     8581 tcacaaaacc ttctgaatct tcacttataa ttcgattggc atcaaaacca tgaccatctt
     8641 cttcctcatg atggacatgt agtgacggat tacttaatac agaactagaa gaacttccta
     8701 cctcttccgt gttagagtgt gatgggggat tgttaagaga tgacttagga atatagtgat
     8761 agtgatcccc atgtcttact atataagcat cacctgtatc tctgacaata tcattagggt
     8821 taaagacata tgtggctgct aattcacctg ccgacaagtc actctcagga atgaaatgat
     8881 agtgaccacc atgtggtact atagtagatt gaaatagaat atgagcaaat tgataagggg
     8941 attttaaagt aatttctaac aatgatttag aaactatgat gtgctattct aaattcaact
     9001 cactatatat aaccatcatc ggtagtataa cgtccctgta attttgctac agatacttct
     9061 gcactagctc ctttatcgtc tttaccatgt tcttgttttt ggcgattgat ttcatctttt
     9121 gttcgtacat tttctgcatg agcttgatct ttaaggtaaa cataatactt tccatctacc
     9181 ttaataatat atcctccctt aacctaactg acgatatctt gatctttcgg ctgatagttg
     9241 ggggctttca ttaatagctc ttcactaaag agcgcatcaa aaggaacttt accattatag
     9301 tagtgataat gatcgccatg agaagttaca taaccttgat ctgtaatctt aataacaatt
     9361 tgttttgctt gaattccttc tttttgacta acctagtctg gagtcaaatt ttcagtcttc
     9421 ttagtgtctt tattactgtt tacatatgaa acacgatttt tatctgtatt ggcctgttag
     9481 ctatgttggt tcagagcata aacacacaga cttaaggaaa ggataacaac agatccagct
     9541 gctatatatt tctttttaaa tttcataatt acctcatttc tataattatt tatatgatgt
     9601 cttcattatt aaatg
//
LOCUS       DAVKNL010000070         9558 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVKNL010000070 DAVKNL010000000
VERSION     DAVKNL010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9558
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.070"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            357..3017
                     /gene="rrgA"
                     /locus_tag="VW411_001802"
     CDS             357..3017
                     /gene="rrgA"
                     /locus_tag="VW411_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus tip adhesin RrgA"
                     /protein_id="HEU9794485.1"
                     /translation="MKKVRKIFQKAVAGLCCISQLTAFSSIVALAETPETSPAIGKVV
                     IKETGEGGALLGDAVFELKNNTDGTTVSQRTEAQTGEAIFSNIKPGTYTLTEAQPPVG
                     YKPSTKQWTVEVEKNGRTTVQGEQVENREEALSDQYPQTGTYPDVQTPYQIIKVDGSE
                     KNGQHKALNPNPYERVIPEGTLSKRIYQVNNLDDNQYGIELTVSGKTVYEQKDKSVPL
                     DVVILLDNSNSMSNIRNKNARRAERAGEATRSLIDKITSDSENRVALVTYASTIFDGT
                     EFTVEKGVADKNGKRLNDSLFWNYDQTSFTTNTKDYSYLKLTNDKNDIVELKNKVPTE
                     AEDHDGNRLMYQFGATFTQKALMKADEILTQQARQNSQKVIFHITDGVPTMSYPINFN
                     HATFAPSYQNQLNAFFSKSPNKDGILLSDFITQATSGEHTIVRGDGQSYQMFTDKTVY
                     EKGAPAAFPVKPEKYSEMKAAGYAVIGDPINGGYIWLNWRESILAYPFNSNTAKITNH
                     GDPTRWYYNGNIAPDGYDVFTVGIGINGDPGTDEATATSFMQSISSKPENYTNVTDTT
                     KILEQLNRYFHTIVTEKKSIENGTITDPMGELIDLQLGTDGRFDPADYTLTANDGSRL
                     ENGQAVGGPQNDGGLLKNAKVLYDTTEKRIRVTGLYLGTDEKVTLTYNVRLNDEFVSN
                     KFYDTNGRTTLHPKEVEQNTVRDFPIPKIRDVRKYPEITISKEKKLGDIEFIKVNKND
                     KKPLRGAVFSLQKQHPDYPDIYGAIDQNGTYQNVRTGEDGKLTFKNLSDGKYRLFENS
                     EPAGYKPVQNKPIVAFQIVNGEVRDVTSIVPQDIPAGYEFTNDKHYITNEPIPPKREY
                     PRTGGIGMLPFYLIGCMMMGGVLLYTRKHP"
     gene            3241..5238
                     /gene="rrgB"
                     /locus_tag="VW411_001803"
     CDS             3241..5238
                     /gene="rrgB"
                     /locus_tag="VW411_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus major pilin RrgB"
                     /protein_id="HEU9794486.1"
                     /translation="MKSINKFLTMLAALLLTASSLFSAATVFAAGTTTTSVTVHKLLA
                     TDGDMDKIANELETGNYAGNKVGVLPANAKEIAGVMFVWTNTNNEIIDENGQTLGVNI
                     DPQTFKLSGAMPATAMKKLTEAEGAKFNTANLPAAKYKIYEIHSLSTYVGEDGATLTG
                     SKAVPIEIELPLNDVVDAHVYPKNTEAKPKIDKDFKGKANPDTPRVDKDTPVNHQVGD
                     VVEYEIVTKIPALANYATANWSDRMTEGLAFNKGTVKVTVDDVALEAGDYALTEVATG
                     FDLKLTDAGLAKVNDQNAEKTVKITYSATLNDKAIVEVPESNDVTFNYGNNPDHGNTP
                     KPNKPNENGDLTLTKTWVDATGAPIPAGAEATFDLVNAQTGKVVQTVTLTTDKNTVTV
                     NGLDKNTEYKFVERSIKGYSADYQEITTAGEIAVKNWKDENPKPLDPTEPKVVTYGKK
                     FVKVNDKDNRLAGAEFVIANADNAGQYLARKADKVSQEEKQLVVTTKDALDRAVAAYN
                     ALTAQQQTQQEKEKVDKAQAAYNAAVIAANNAFEWVADKDNENVVKLVSDAQGRFEIT
                     GLLAGTYYLEETKQPAGYALLTSRQKFEVTATSYSATGQGIEYTAGSGKDDATKVVNK
                     KITIPQTGGIGTIIFAVAGAAIMGIAVYAYVKNNKDEDQLA"
     gene            5285..6466
                     /gene="rrgC"
                     /locus_tag="VW411_001804"
     CDS             5285..6466
                     /gene="rrgC"
                     /locus_tag="VW411_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus minor pilin RrgC"
                     /protein_id="HEU9794487.1"
                     /translation="MISRIFFVMALCFSLVWGAHAVQAQEDHTLVLQLENYQEVVSQL
                     PSRDGHRLQVWKLDDSYSYDDRVQIVRDLHSWDENKLSSFKKTSFEMTFLENQIEVSH
                     IPNGLYYVRSIIQTDAVSYPAEFLFEMTDQTVEPLVIVAKKTDTMTTKVKLIKVDQDH
                     NRLEGVGFKLVSVARDVSEKEVPLIGEYRYSSSGQVGRTLYTDKNGEIFVTNLPLGNY
                     RFKEVEPLAGYAVTTLDTDVQLVDHQLVTITVVNQKLPRGNVDFMKVDGRTNTSLQGA
                     MFKVMKEESGHYTPVLQNGKEVVVTSGKDGRFRVEGLEYGTYYLWELQAPTGYVQLTS
                     PVSFTIGKDTRKELVTVVKNNKRPRIDVPDTGEETLYILMLVAILLFGSGYYLTKKPN
                     N"
     gene            6804..7706
                     /gene="srtC-1"
                     /locus_tag="VW411_001805"
     CDS             6804..7706
                     /gene="srtC-1"
                     /locus_tag="VW411_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-1"
                     /protein_id="HEU9794488.1"
                     /translation="MVKTKKQKRNNLLLGVVFFIGMAVMAYPLVSRLYYRVESNQQIA
                     DFDKEKATLDEADIDERMKLAQAFNDSLNNVVSGDPWSEEMKKKGRAEYARMLEIHER
                     MGHVEIPVIDVDLPVYAGTAEEVLQQGAGHLEGTSLPIGGNSTHAVITAHTGLPTAKM
                     FTDLTKLKVGDKFYVHNIKEVMAYQVDQVKVIEPTNFDDLLIVPGHDYVTLLTCTPYM
                     INTHRLLVRGHRIPYVAEVEEEFIAANKLSHLYRYLFYVAVGLIVILLWIIRRLRKKK
                     KQPEKALKALKAARKEVKVEDGQQ"
     gene            7693..8586
                     /gene="srtC-2"
                     /locus_tag="VW411_001806"
     CDS             7693..8586
                     /gene="srtC-2"
                     /locus_tag="VW411_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-2"
                     /protein_id="HEU9794489.1"
                     /translation="MDNSRRSRKKGTKKKKHPLILLLIFLVGFAVAIYPLVSRYYYRI
                     ESNEVIKEFDETVSQMDKAELEERWRLAQAFNATLKPSEILDPFTEQEKKKGVSEYAN
                     MLKVHERIGYVEIPAIDQEIPMYVGTSEDILQKGAGLLEGASLPVGGENTHTVITAHR
                     GLPTAELFSQLDKMKKGDIFYLHVLDQVLAYQVDQIVTVEPNDFEPVLIQHGEDYATL
                     LTCTPYMINSHRLLVRGKRIPYTAPIAERNRAVRERGQFWLWLLLGAMAVILLLLYRV
                     YRNRRIVKGLEKQLEGRHVKD"
     gene            8573..9424
                     /gene="srtC-3"
                     /locus_tag="VW411_001807"
     CDS             8573..9424
                     /gene="srtC-3"
                     /locus_tag="VW411_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-3"
                     /protein_id="HEU9794490.1"
                     /translation="MSRTKLRALLGYLLMLVACLIPIYCFGQMVLQSLGQVKGHATFV
                     KSMTTEMYQEQQNHSLAYNQRLASQNRIVDPFLAEGYEVNYQVSDDPDAVYGYLSIPS
                     LEIMEPVYLGADYHHLGMGLAHVDGTPLPLDGTGIRSVIAGHRAEPSHVFFRHLDQLK
                     VGDALYYDNGQEIVEYQMMDTEIILPSEWEKLESVSSKNIMTLITCDPIPTFNKRLLV
                     NFERVAVYQKSDPQTAAVARVAFTKEGQSVSRVATSQWLYRGLVVLAFLGILFVLWKL
                     ARLLRGK"
BASE COUNT         3100 a         1562 c         2224 g         2672 t
ORIGIN      
        1 atggcttatt ttttttatta gaaaatattt ttttatcaaa tattgtcgtt ctataaaaaa
       61 atatgtgata aaaatatcta ttgtgatgga agttgtttta atttatacta ggatagttaa
      121 tagtaatact atactatact atattgtata caagtgtgtc attgccaggt tgagaagata
      181 gctataacgc acttttatac gcttttgcta cgtttgttag tgaacggatt aactcagtga
      241 gataaatttt atcagaacat aagtaatccg tttcttcgtg tatacagatt gaaagtacct
      301 atgaatcata gaaggattaa cttgttctat gaataatgct taacagggag acacacatga
      361 aaaaagtaag aaagatattt cagaaggcag ttgcaggact gtgctgtata tctcagttga
      421 cagctttttc ttcgatagtt gctttagcag aaacgcctga aaccagtcca gcgataggaa
      481 aagtagtgat taaggagaca ggcgaaggag gagcgcttct aggagatgcc gtctttgagt
      541 tgaaaaacaa tacggatggc acaactgttt cgcaaaggac agaggcgcaa acaggagaag
      601 cgatattttc aaacataaaa cctgggacat acaccttgac agaagcccaa cctccagttg
      661 gttataaacc ctctactaaa caatggactg ttgaagttga gaagaatggt cggacgactg
      721 tccaaggtga acaggtagaa aatcgagaag aggctctatc tgaccagtat ccacaaacag
      781 ggacttatcc agatgttcaa acaccttatc agattattaa ggtagatggt tcggaaaaaa
      841 acggacagca caaggcgttg aatccgaatc catatgaacg tgtgattcca gaaggtacac
      901 tttcaaagag aatttatcaa gtgaataatt tggatgataa ccaatatgga atcgaattga
      961 cggttagtgg gaaaacagtg tatgaacaaa aagataagtc tgtgccgctg gatgtcgtta
     1021 tcttgctcga taactcaaat agtatgagta acattcgaaa caagaatgct cgacgtgcgg
     1081 aaagagctgg tgaggcgaca cgttctctta ttgataaaat tacatctgat tcagaaaata
     1141 gggtagcgct tgtgacttat gcttccacta tctttgatgg gaccgagttt acagtagaaa
     1201 aaggggtagc agataaaaac ggaaagcgat tgaatgattc tcttttttgg aattatgatc
     1261 agacgagttt tacaaccaat accaaagatt atagttattt aaagctgact aatgataaga
     1321 atgacattgt agaattaaaa aataaggtac ctaccgaggc agaagaccat gatggaaata
     1381 gattgatgta ccaattcggt gccactttta ctcagaaagc tttgatgaag gcagatgaga
     1441 ttttgacaca acaagcgaga caaaatagtc aaaaagtcat tttccatatt acggatggtg
     1501 tcccaactat gtcgtatccg attaatttta atcatgctac gtttgctcca tcatatcaaa
     1561 atcaactaaa tgcatttttt agtaaatctc ctaataaaga tggaatacta ttaagtgatt
     1621 ttattacgca agcaactagt ggagaacata caattgtacg cggagatggg caaagttacc
     1681 agatgtttac agataagaca gtttatgaaa aaggtgctcc tgcagctttc ccagttaaac
     1741 ctgaaaaata ttctgaaatg aaggcggctg gttatgcagt tataggcgat ccaattaatg
     1801 gtggatatat ttggcttaat tggagagaga gtattctggc ttatccgttt aattctaata
     1861 ctgctaaaat taccaatcat ggtgacccta caagatggta ctataacggg aatattgctc
     1921 ctgatgggta tgatgtcttt acggtaggta ttggtattaa cggagatcct ggtacggatg
     1981 aagcaacggc tactagtttt atgcaaagta tttctagtaa acctgaaaac tataccaatg
     2041 ttactgacac gacaaaaata ttggaacagt tgaatcgtta tttccacacc atcgtaactg
     2101 aaaagaaatc aattgagaat ggtacgatta cagatccgat gggtgagtta attgatttgc
     2161 aattgggcac agatggaaga tttgatccag cagattacac tttaactgca aacgatggta
     2221 gtcgcttgga gaatggacaa gctgtaggtg gtccacaaaa tgatggtggt ttgttaaaaa
     2281 atgcaaaagt gctctatgat acgactgaga aaaggattcg tgtaacaggt ctgtaccttg
     2341 gaacggatga aaaagttacg ttgacctaca atgttcgttt gaatgatgag tttgtaagca
     2401 ataaatttta tgataccaat ggtcgaacaa ccttacatcc taaggaagta gaacagaaca
     2461 cagtgcgcga cttcccgatt cctaagattc gtgatgtgcg gaagtatcca gaaatcacaa
     2521 tttcaaaaga gaaaaaactt ggtgacattg agtttattaa ggtcaataaa aatgataaaa
     2581 aaccactgag aggtgcggtc tttagtcttc aaaaacaaca tccggattat ccagatattt
     2641 atggagctat tgatcaaaat ggcacttatc aaaatgtgag aacaggtgaa gatggtaagt
     2701 tgacctttaa aaatctgtca gatgggaaat atcgattatt tgaaaattct gaaccagctg
     2761 gttataaacc cgttcaaaat aagcctatcg ttgccttcca aatagtaaat ggagaagtca
     2821 gagatgtgac ttcaatcgtt ccacaagata taccagcggg ttacgagttt acgaatgata
     2881 agcactatat taccaatgaa cctattcctc caaagagaga atatcctcga actggtggta
     2941 tcggaatgtt gccattctat ctgataggtt gcatgatgat gggaggagtt ctattataca
     3001 cacggaaaca tccgtaaagt gtagaaatga taatatctat gttctgaacg atacttttaa
     3061 gaagtagcac tcaagaagag atttaagttt acttggtgaa acctgtttta ttcgtaagta
     3121 aactatcatt gaaaggggag atgttttcga aaacttgcac agaaaaagga ttattattgt
     3181 catgtgtaat tcattacatt gctcacagtt gattttaaga gatatgaata aggagaaatc
     3241 atgaaatcaa tcaacaaatt tttaacaatg cttgctgcct tattactgac agcgagtagc
     3301 ctgttttcag ctgcaacagt ttttgcggct gggacgacaa caacatctgt taccgttcat
     3361 aaactattgg caacagatgg ggatatggat aaaattgcaa atgagttaga aacaggtaac
     3421 tatgctggta ataaagtggg tgttctacct gcaaatgcaa aagaaattgc cggtgttatg
     3481 ttcgtttgga caaatactaa taatgaaatt attgatgaaa atggccaaac tctaggagtg
     3541 aatattgatc cacaaacatt taaactctca ggggcaatgc cggcaactgc aatgaaaaaa
     3601 ttaacagaag ctgaaggagc taaatttaac acggcaaatt taccagctgc taagtataaa
     3661 atttatgaaa ttcacagttt atcaacttat gtcggtgaag atggagcaac cttaacaggt
     3721 tctaaagcag ttccaattga aattgaatta ccattgaacg atgttgtgga tgcgcatgtg
     3781 tatccaaaaa atacagaagc aaagccaaaa attgataaag atttcaaagg taaagcaaat
     3841 ccagatacac cacgtgtaga taaagataca cctgtgaacc accaagttgg agatgttgta
     3901 gagtacgaaa ttgttacaaa aattccagca cttgctaatt atgcaacagc aaactggagc
     3961 gatagaatga ctgaaggttt ggcattcaac aaaggtacag tgaaagtaac tgttgatgat
     4021 gttgcacttg aagcaggtga ttatgctcta acagaagtag caactggttt tgatttgaaa
     4081 ttaacagatg ctggtttagc taaagtgaat gaccaaaacg ctgaaaaaac tgtgaaaatc
     4141 acttattcgg caacattgaa tgacaaagca attgtagaag taccagaatc taatgatgta
     4201 acatttaact atggtaataa tccagatcac gggaatactc caaagccgaa taagccaaat
     4261 gaaaacggcg atttgacatt gaccaagaca tgggttgatg ctacaggtgc accaattccg
     4321 gctggagctg aagcaacgtt cgatttggtt aatgctcaga ctggtaaagt tgtacaaact
     4381 gtaactttga caacagacaa aaatacagtt actgttaacg gattggataa aaatacagaa
     4441 tataaattcg ttgaacgtag tataaaaggg tattcagcag attatcaaga aatcactaca
     4501 gctggagaaa ttgctgtcaa gaactggaaa gacgaaaatc caaaaccact tgatccaaca
     4561 gagccaaaag ttgttacata tggtaaaaag tttgtcaaag ttaatgataa agataatcgt
     4621 ttagctgggg cagaatttgt aattgcaaat gctgataatg ctggtcaata tttagcacgt
     4681 aaagcagata aagtgagtca agaagagaag cagttggttg ttacaacaaa ggatgcttta
     4741 gatagagcag ttgctgctta taacgctctt actgcacaac aacaaactca gcaagaaaaa
     4801 gagaaagttg acaaagctca agctgcttat aatgctgctg tgattgctgc caacaatgca
     4861 tttgaatggg tggcagataa ggacaatgaa aatgttgtga aattagtttc tgatgcacaa
     4921 ggtcgctttg aaattacagg ccttcttgca ggtacatatt acttagaaga aacaaaacag
     4981 cctgctggtt atgcattact aactagccgt cagaaatttg aagtcactgc aacttcttat
     5041 tcagcgactg gacaaggcat tgagtatact gctggttcag gtaaagatga cgctacaaaa
     5101 gtagtcaaca aaaaaatcac tatcccacaa acgggtggta ttggtacaat tatctttgct
     5161 gtagcggggg ctgcgattat gggtattgca gtgtacgcat atgttaaaaa caacaaagat
     5221 gaggatcaac ttgcttaagt aagagagaaa ggagccattg atgacaatgc agaaaatgca
     5281 gaaaatgatt agtcgtatct tctttgttat ggctctgtgt ttttctcttg tatggggtgc
     5341 acatgcagtc caagcgcaag aagatcacac gttggtcttg caattggaga actatcagga
     5401 ggtggttagt caattgccat ctcgtgatgg tcatcggttg caagtatgga agttggatga
     5461 ttcgtattcc tatgatgatc gggtgcaaat tgtaagagac ttgcattcgt gggatgagaa
     5521 taaactttct tctttcaaaa agacttcgtt tgagatgacc ttccttgaga atcagattga
     5581 agtatctcat attccaaatg gtctttacta tgttcgctct attatccaga cggatgcggt
     5641 ttcttatcca gctgaatttc tttttgaaat gacagatcaa acggtagagc ctttggtcat
     5701 tgtagcgaaa aaaacagata caatgacaac aaaggtgaag ctgataaagg tggatcaaga
     5761 ccacaatcgc ttggagggtg tcggctttaa attggtatca gtagcaagag atgtttctga
     5821 aaaagaggtt cccttgattg gagaataccg ttacagttct tctggtcaag tagggagaac
     5881 tctctatact gataaaaatg gagagatttt tgtgacaaat cttcctcttg ggaactatcg
     5941 tttcaaggag gtggagccac tggcaggcta tgctgttacg acgctggata cggatgtcca
     6001 gctggtagat catcagctgg tgacgattac ggttgtcaat cagaaattac cacgtggcaa
     6061 tgttgacttt atgaaggtgg atggtcggac caatacctct cttcaagggg caatgttcaa
     6121 agtcatgaaa gaagaaagcg gacactatac tcctgttctt caaaatggta aggaagtagt
     6181 tgtaacatca gggaaagatg gtcgtttccg agtggaaggt ctagagtatg ggacatacta
     6241 tttatgggag ctccaagctc caactggtta tgttcaatta acatcgcctg tttcctttac
     6301 aatcgggaaa gatactcgta aggaactggt aacagtggtt aaaaataaca agcgaccacg
     6361 gattgatgtg ccagatacag gggaagaaac cttgtatatc ttgatgcttg ttgccatttt
     6421 gttgtttggt agtggttatt atcttacgaa aaaaccaaat aactgatatt caatgtacat
     6481 cattatgaaa aagatagcag gctgaaggga agaccagagt actctgaggt gatgttaatc
     6541 aggaatcatg gtgatgtggc atgaatcaca ataacggata tgaggctggg cagattgtgc
     6601 cagcctcatt gtgggttatt gtttgtaaaa cgataggact ggtctggtaa tcattttagg
     6661 aatggacagg actgggattc tgatttaaaa tggatggtga atcagaaaga aatgagattt
     6721 tctcgtttct cttagcagat aggattgtct gttaggaaaa gcgataaaat gatgagtttg
     6781 aagataaagg gatgctgata aaaatggtaa aaacaaaaaa gcaaaaacga aataatctcc
     6841 tattaggagt ggtatttttc attggaatgg cggtaatggc gtatccgctg gtgtctcgct
     6901 tgtattatcg agtggaatca aatcaacaaa ttgctgactt tgataaggaa aaagcaacgt
     6961 tggatgaggc tgacattgat gaacgaatga aattggcaca agccttcaat gactctttga
     7021 ataatgtagt gagtggcgat ccttggtcgg aagaaatgaa gaaaaaaggg cgagcagagt
     7081 atgcacgtat gttagaaatc catgagcgga tggggcatgt ggaaatcccc gttattgacg
     7141 tggatttgcc ggtttatgct ggtactgctg aagaggtatt gcagcaaggg gctgggcatc
     7201 tagagggaac ttctctgccg atcggaggca attcgaccca tgcggtgatt acggcacata
     7261 caggtttgcc aacagctaag atgtttacgg atttgaccaa acttaaagtt ggggataagt
     7321 tttatgtgca caatatcaag gaagtgatgg cctatcaagt ggatcaagta aaggtgattg
     7381 agccgacgaa ctttgatgat ttattgattg taccaggtca tgattatgtg accttgctga
     7441 cttgtacgcc atacatgatc aatacccatc gtctattggt tcgggggcat cggataccgt
     7501 acgtagcaga ggttgaggaa gaatttattg cagcaaacaa actcagtcat ctctatcgct
     7561 acctgtttta tgtggcagtt ggtttgattg tgattctttt atggattatt cgacgcttgc
     7621 gcaagaagaa aaaacaaccg gaaaaggctt tgaaggcgct gaaagcagca aggaaggaag
     7681 tgaaggtgga ggatggacaa cagtagacgt tcacgaaaaa aaggcacaaa aaagaagaaa
     7741 catccgctga tccttcttct gattttctta gtaggattcg ccgttgcgat atatccattg
     7801 gtgtctcgtt attattatcg tattgagtca aacgaggtta ttaaagagtt tgatgagacg
     7861 gtttcccaga tggataaggc agaacttgag gagcgttggc gcttggctca agccttcaat
     7921 gcgaccttga aaccatctga aattcttgat ccttttacag agcaagagaa aaagaaaggc
     7981 gtctcagaat atgccaatat gctaaaggtc catgagcgga ttggctatgt ggaaattcct
     8041 gcgattgatc aggaaattcc gatgtatgtc ggaacgagtg aggacattct tcagaaaggg
     8101 gcagggctgt tagaaggggc ttcgctgcct gttggaggtg aaaataccca tacagtgatc
     8161 actgctcaca gaggattgcc aacggcagaa ttgttcagtc aattggataa gatgaaaaaa
     8221 ggggatatct tttatcttca cgttttagat caggtgttgg cctaccaagt ggatcagata
     8281 gtgacggtgg agccgaatga ctttgagcct gtcttgattc aacatgggga agattatgcg
     8341 accttgttga cttgtacacc gtatatgatt aacagtcatc gtctgttggt acgtgggaag
     8401 cggattccgt atacggcacc aattgcagag cggaatcgag cggtgagaga gcgtgggcaa
     8461 ttctggttgt ggttattact aggagcgatg gcggtcatcc ttctcttgct gtatcgcgtg
     8521 tatcgtaatc gacggattgt caaaggacta gaaaagcaat tggaggggcg tcatgtcaag
     8581 gactaaacta cgagccttat tgggatactt gttgatgttg gtagcctgtt tgattcctat
     8641 ttattgtttt ggacagatgg tgttgcagtc tcttggacag gtgaaaggtc atgctacatt
     8701 tgtgaaatcc atgacaactg aaatgtacca agaacaacag aaccattctc tcgcctacaa
     8761 tcaacgcttg gcttcgcaaa atcgcattgt agatcctttt ttggcggagg gatatgaggt
     8821 caattaccaa gtgtctgacg accctgatgc agtctatggt tacttgtcta ttccaagttt
     8881 ggaaatcatg gagccggttt atttgggagc agattatcat catttaggga tgggcttggc
     8941 tcatgtggat ggtacaccgc tgcctctgga tggtacaggg attcgctcag tgattgctgg
     9001 gcaccgtgca gagccaagcc atgtcttttt ccgccatttg gatcagctaa aagttggaga
     9061 tgctctttat tatgataatg gccaggaaat tgtagaatat cagatgatgg acacagagat
     9121 tattttaccg tcggaatggg aaaaattaga atcggttagc tctaaaaata tcatgacctt
     9181 gataacctgc gatccgattc ctacctttaa taaacgctta ttagtgaatt ttgaacgagt
     9241 cgctgtttat caaaaatcag atccacaaac agctgcagtt gcgagggttg cttttacgaa
     9301 agaaggacaa tctgtatcgc gtgttgcaac ctctcaatgg ttgtaccgtg ggctagtggt
     9361 actggcattt ctgggaatcc tgtttgtttt gtggaagcta gcacgtttac tacgagggaa
     9421 ataaaaagaa atgaaaggaa agctaaggct gttccttttt ccggctcttt gtcaactgta
     9481 gtgggttgaa aaaaagctaa gctcgagaaa ggacaaattt tgtcctttct tttttgatat
     9541 tcagagcgat aaaaatcc
//
LOCUS       DAVKNL010000071         9536 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVKNL010000071 DAVKNL010000000
VERSION     DAVKNL010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9536
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.071"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            507..1793
                     /locus_tag="VW411_001808"
     CDS             507..1793
                     /locus_tag="VW411_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU9794491.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAVTEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESPEKFEAIKQDLVDEMWKVAQRELATGFYYGTPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAAQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQSGDMVRALKEGLINLYKEDGTSVTVRA"
     gene            1808..2119
                     /locus_tag="VW411_001809"
     CDS             1808..2119
                     /locus_tag="VW411_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794492.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LEHHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
     gene            2365..3093
                     /locus_tag="VW411_001810"
                     /pseudo
     CDS             2365..3093
                     /locus_tag="VW411_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
     gene            3428..4162
                     /locus_tag="VW411_001811"
     CDS             3428..4162
                     /locus_tag="VW411_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9794493.1"
                     /translation="MRIKEKTNNINGGIKNVSKHYGHSIILKDINFALNKGEIVGLAG
                     RNGVGKSTLMKILVQNNQPTSGNIISSDNVGYLIEEPKLFLSKTGLENLKYLSNLYGV
                     DYNQERFRCLIQELDLTQSINKKVKTYSLGTKQKLALLLTLVTEPDILILDEPTNGLD
                     IESSQIVLAVLKKLALHENVGILISSHKLEDIEEICERVLFLENGLLTFQKVGKDSHN
                     FLFEIAFSSATDRDIFITKQEFWDIV"
     gene            4322..4802
                     /locus_tag="VW411_001812"
                     /pseudo
     CDS             4322..4802
                     /locus_tag="VW411_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5187..5396
                     /locus_tag="VW411_001813"
     CDS             5187..5396
                     /locus_tag="VW411_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9794494.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            5386..5874
                     /locus_tag="VW411_001814"
     CDS             5386..5874
                     /locus_tag="VW411_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794495.1"
                     /translation="MKNRFYYSQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYGRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYIIYLPWIFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            5990..7450
                     /locus_tag="VW411_001815"
     CDS             5990..7450
                     /locus_tag="VW411_001815"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEU9794496.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            7447..8271
                     /gene="nadE"
                     /locus_tag="VW411_001816"
     CDS             7447..8271
                     /gene="nadE"
                     /locus_tag="VW411_001816"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEU9794497.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSAFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            8621..9172
                     /locus_tag="VW411_001817"
     CDS             8621..9172
                     /locus_tag="VW411_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9794498.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDVEITRNSICNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGNGMMTETLKAILDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
BASE COUNT         2984 a         1600 c         2010 g         2942 t
ORIGIN      
        1 agctatatct attataacat ggtttaataa attctttgag cttttttctt tttaaatatt
       61 gattgtttct aaaagttcta gtaatattgc tatttctcat ttttccaaat atgttattca
      121 acttattagc tacgtctcta ttcatatttg ggtataaaac aaaaaatcaa ttctaacagt
      181 tttatatcag aataataaat aaaaaagcga tattctagac tgtatcaatc cttttccatt
      241 ataggagcaa cagagtggga gtagtcatct aaggactaat ttatgtattt ttacgagtca
      301 gtatcttggg atactggttt ttacttttct agactttttg actacttgtt aaaactggga
      361 taattttcga ctgtttaaca gttattatgc aaagtctaaa agattagaat tgtcaaaaca
      421 atccgtctag gcttgatttt atcctttatt tactataaaa tgagaaggaa aaatgtcaaa
      481 cttttatatt gcaaatagga gaaatcatga caaaaacatt aaaacgtcct gaggttttat
      541 cacctgcagg gactttagag aagctaaagg tagctgttca gtatggagca gatgctgtct
      601 ttatcggtgg tcaggcctat ggtcttcgta gccgtgcggg aaactttact ttcgaacaga
      661 tggaagaagg cgtgcagttt gcggccaagt atggtgccaa ggtctatgta gcggctaata
      721 tggttatgca cgaaggaaat gaagctggtg ctggtgagtg gttccgtaaa ctgcgtgata
      781 tcgggattgc agcagttatc gtatctgacc cagccttgat tatgattgca gtgactgaag
      841 caccaggcct tgaaatccac ctttctaccc aagccagtgc cactaactat gaaacccttg
      901 agttctggaa agagctaggc ttgactcgtg tcgttttagc gcgtgaggtt tcaatggaag
      961 aattagctga gatccgcaaa cgtacagatg ttgaaattga agcctttgtc catggagcta
     1021 tgtgtatttc atactctgga cgttgtactc tttcaaacca catgagtatg cgtgatgcca
     1081 accgtggtgg atgttctcag tcatgccgtt ggaaatacga cctttacgat atgccatttg
     1141 ggaaagaacg taagagtttg cagggtgaga ttccagaaga attttcaatg tcagccgttg
     1201 acatgtctat gattgaccac attccagata tgattgaaaa tggtgtggac agtctaaaaa
     1261 tcgaaggacg tatgaagtct attcactacg tatcaacagt aaccaactgc tacaaggcgg
     1321 ctgtggatgc ctatcttgaa agtcctgaaa agtttgaagc tatcaaacaa gacttggtgg
     1381 acgagatgtg gaaggttgcc caacgtgaac tggctacagg attttactat ggtacaccat
     1441 ctgaaaatga gcagttgttt ggtgctcgcc gtaaaattcc tgagtacaag tttgtcgctg
     1501 aagtggtttc ttatgatgat gcggcacaaa cagcaacaat tcgtcaacga aatgtcatta
     1561 acgaagggga ccaagttgag ttttatggtc caggtttccg tcattttgaa acctatattg
     1621 aagatttgca tgatgccaaa ggcaataaaa tcgaccgcgc tccaaatcca atggaactat
     1681 tgactattaa ggtgcctcaa cccgttcaat caggagatat ggttcgtgca ttaaaagaag
     1741 gactcatcaa tctttataag gaagatggaa ccagcgtcac agttcgagct taagaaagga
     1801 aaaggaaatg atagaggcac agggtttctt agtggataag caaacaagat gcattcatta
     1861 ccatagcaag ctggatatta ttgctttaca atgctatgat tgtaaaaagt attatgcttg
     1921 ttatcggtgt catgattcat tagaacatca cccttttgag ccgtatccct tatctttgat
     1981 acaggataag cctattttat gtggtgtttg tctaaaacta ctaacatata agcaatataa
     2041 agaaagctta agttgcccct tttgtttttc tcgctttaat ccaggttgcc aaaatcataa
     2101 ggaacgctat tttaaatagc aaatcatcta gttttgaagt aggagaaaac tcaatttcaa
     2161 gagaaaatga agtaaatctt cccacaataa aacgcataat atcaagattg ttcaatacct
     2221 gatactatgc gtttttaaga ttttaaagac ttttttcctt tatctggtat tttgactact
     2281 tgttaaaact gggttaattt tcgactgttt aatagttatt atgcaaagtc taaaaggtta
     2341 gaattgtcaa aacaatccgt ctagagtatg cgtgatgcca accgtggtgg atgttctcag
     2401 tcatgccgtt ggaagtacga cctttacgat atgccatttg ggaaagaacg taagagtttg
     2461 cagggtgaga ttccagaaga attttcaatg tcagccgttg atatgtctat gattgaccat
     2521 atctcagata tgattgaaaa tggtgtggac agtctaaaaa tcgaaggacg tatggagtct
     2581 attcactatg tatcaacagt aaccaactgc tacaaggcgg ctgtggatgc ctatcttgaa
     2641 agtcctgaaa agtttgaagc tatcaaacaa gacttggtgg acgagatgtg gaaggttgcc
     2701 caacgtgaac tggctacagg attttactat ggtacaccat ctgaaaatga gcagttgttt
     2761 ggtgctcgtc gtaaaatccc tgagtacaag tttgtcgctg aagtggtttc ttatgatgat
     2821 gcggcacaaa cagcaactat tcgtcaacga aacgtcatta acgaagggga ccaagttgag
     2881 ttttatggtc caggtttccg tcattttgaa acctatattg aagatttgca tgatgctaaa
     2941 ggcaataaaa tcgaccgcgc tccaaatcca atggaactat tgactattaa agtcccacaa
     3001 cctgttcaat caggagacat ggttcgagct cttaaagagg ggcttatcaa tctttataag
     3061 gaagatggaa ccagcgtcac agttcgtgct taatgtagtt gtttagtttt aaaaaactat
     3121 gcaaagctcc atatacaaca cttaaacgag attaaagaat ggcgaaatcc cttgatgcgc
     3181 aagagattag ctgtcttttt tattttttaa gtgataaagt cggagtttag gcatcaaagc
     3241 ctatcaaatt aaacaaagaa gcgatgtctt agatattttg aaaaaaatta ataagcagaa
     3301 aactctctat tattttgttg tagagagttt tttgttaata aaatttcaca aaatgacatt
     3361 tatatattgc attaagttag atatatgata taattttgtt aaaaagaggc gcaacttttt
     3421 aaaattaatg agaatcaaag agaaaaccaa taatattaat ggaggaataa aaaatgtaag
     3481 taagcattat ggtcattcaa tcattctcaa agatataaat tttgcactta acaagggtga
     3541 aattgttggt ctagcaggga gaaatggagt tggtaagagt acgttgatga aaattcttgt
     3601 tcagaataat caaccgactt caggtaatat tataagcagt gataatgttg ggtatttaat
     3661 cgaagaacca aaattatttt tatctaaaac aggtttagag aatttaaaat atttgtcaaa
     3721 tttatatggt gttgactaca atcaagaaag atttagatgt ttgatccaag agttagattt
     3781 gactcagtct attaataaaa aagtaaagac ctattctttg ggtacaaaac aaaaattagc
     3841 tttgcttcta actctcgtta cggaacctga tatattgatt ttagatgaac cgactaatgg
     3901 tttagatatt gaatcatcac aaatagtttt agcggttcta aaaaaattag ctttacatga
     3961 aaatgtggga attttaatat cgagtcataa attagaagac attgaagaaa tttgtgagag
     4021 agttcttttc ttggagaacg ggcttttgac atttcaaaaa gtaggaaaag atagtcataa
     4081 tttcttgttt gagatagctt tttcatcagc tacagataga gacattttca ttaccaaaca
     4141 agaattttgg gatattgttt aggaagaggg attgagaatt actatgtctg ggaatattca
     4201 aaatagtgag ctttttaaat tttttaacga aaactctatt aaagtagttg attttgaaac
     4261 taaaaaagag acgcttaaag atatttacct aaatcgttca aaataaagga aggttataat
     4321 catgaaatta aataaacaga agaatcggat gatttacgtc ttgtctaatt ttctatatgc
     4381 tatctcagtt tccattattt atgctttgaa tggcattgtg ttactagtca tagtaagtaa
     4441 attgggtatt ccaggtgatt taggattaaa ttttatagta gctattgtag tcaatacaat
     4501 tttgttagtc ctgttttatt ttctattatc ttacattttc tatttataca aattgaaaag
     4561 tggcttggta tttggtattt tagtagcttt actactcttt atctctaata tattaaatac
     4621 gatgatgatg aatactagta atgatttgtt tatcaaagca attgaacttc ttcctttttt
     4681 attctttacc tgtatttgtg gcttcaaata cgatgtctat tagtcagtat cttgtgctaa
     4741 tcactacaat cattttattg tactttttca ctctcaagaa aagcaagaag tattcatttt
     4801 agtttcattt agtattattt tgcataccta aaatacagta aaaaatcagt catcttggta
     4861 tgctcctgct ttcactattc aacacgtttt tgacttatac taggctcatt tccaaaagca
     4921 ttatataata gtgatatgaa accaactaaa ctaaacaaga aatataagca ataaaaattc
     4981 gtttaaaaga tcttactaaa gctaatacta aataaaaata aaagagtaaa ctaggaagtt
     5041 tatttcaaac aacctaaaat actgattttc ggctgaagat aatactggag tgcaaattaa
     5101 tggggttata ataaatagct gatagcttgt gttggttttg gattttttaa gagtagatga
     5161 gtattaaaac tataaggagg acgaaggtgg ctaaaaattt aaaattaaaa ttagctcggg
     5221 tagagcgtga tttaacacaa ggtcaactgg cagaggctgt cggggtgaca cgccagacta
     5281 ttggtttaat agaggcggga aaatacaatc ccagtctctc gctctgccag tctatttgca
     5341 gatgtttagg gaaaacccta gaccaactat tttgggagga agaagatgaa aaatagattt
     5401 tattattctc aattactaga cgaaagagaa gaacaactgt tcaataaagc gggctctgaa
     5461 agtttctata tctgcattgc tttgtcgctc ctatcttata tcatttcagt attagcacca
     5521 agccttttta attctaatat gctgctaatc gttatcatca tagggacatt ttactttttc
     5581 aatcgtgccc gttatctggg agtgacctac tatggtcgtt ttcattttac gattttgggt
     5641 tgttttttcc taaccttggc tattacggct cttttgatgt tgcagaatta tcaattcaac
     5701 atagaaattt atcagcacaa tcctttgaat tttaaatacc tgtctgcttg ggtcattact
     5761 tatatcattt accttccgtg gatctttatt ggcaatcttg gtcttaagag ctatggcgaa
     5821 tgggctcaga aaaaatttga acaagatatg gatgaattgg agagtggaga atagcttgtt
     5881 actcttttct caatccagct aaaatgtgat ataatagtac taatttattg gaatacatga
     5941 aagttcttga aaattttcat gggtttctag ctaaggaagt aggaaaagta tgtatccaga
     6001 tgatagtttg acattgcaca cggacttgta ccagatcaac atgatgcagg tttactttga
     6061 ccaagggatt cacaataaga aggcggtctt tgaggtgtat ttccgccaac agccttttaa
     6121 gaacggctat gcggtttttg caggtttaga aagaattgtg aactatcttg aagacttgcg
     6181 tttttcagat agtgatatag cctatttgga gtcgcttggt tatcatgggg cgttcttgga
     6241 ttaccttcgc aatttcaagt tggagttgac cgttcgttct gcccaagaag gggatttggt
     6301 ttttgctaat gaaccgattg tgcaggtgga aggacctcta gcccaatgtc agttggtcga
     6361 aacggctctt ttgaacatcg tcaactacca gactttggtg gcgacgaagg cagctcgtat
     6421 tcgttcggtt atcgaagatg aacccttgat ggagtttggg acacgtcggg ctcaagaaat
     6481 ggatgcggcc atctggggaa cacgcgcagc tgtgattggt ggcgccaatg gaaccagcaa
     6541 cgtgcgtgcg ggtaagctct ttgacattcc tgttttggga acccatgccc atgccttggt
     6601 acaggtttat ggcaatgact atgaagcttt caaggcttac gctgcgaccc acaaaaattg
     6661 tgtctttctt gtggatacct atgacaccct tcgcatcggt gtaccagctg ccattcaggt
     6721 ggcgcgtgag ctgggtgatc agattaactt tatgggtgtg cggattgact ctggggatat
     6781 tgcctacatt tctaagaaag tccgtcagca actggatgag gctggattta cagaggctaa
     6841 gatttatgct tctaatgatc tagatgaaaa taccatcctt aacctcaaga tgcaaaaggc
     6901 caagattgat gtctggggtg tgggtaccaa gctgattaca gcctatgacc agccggctct
     6961 tggggcggtt tacaagattg ttgcaatcga agatgaaact ggtcagatgc gcaatacgat
     7021 taagctgtct aataatgctg aaaaagtttc tacgccaggt aagaagcagg tgtggcgcat
     7081 taccagtcgt gaaaaaggca agtcagaagg cgactatatc acttatgatg gtgtggatat
     7141 tagcgacatg acagaaatca agatgttcca tccgacctat acatacatca agaagacggt
     7201 tcgtaatttt gatgccgttc ctctcttggt ggatatcttc aaagaaggaa tattagttta
     7261 caacttgcct agtttgactg acattcagga ttatgcccgt aaggaatttg acaagttgtg
     7321 ggatgagtat aagcgtgtgc tcaatccgca gcactatcca gtggatttgg cgcgtgatgt
     7381 atggcaagat aagatggact tgattgataa gatgcgcaag gaagcccttg gtgaaggaga
     7441 agaagaatga gtttgcaaga aacgattatc caagagctgg gtgtcaaacc agtgattgat
     7501 gcccaggaag aaatccgtcg ttctattgat ttcttaaaaa gatatctgaa aaaacatccc
     7561 ttcctaaaaa cctttgtact agggatttct gggggacaag actcaacctt ggcaggacgt
     7621 ttggcgcaat tagctatgga agaactgcga gctgaaacgg gagacgatag ctacaaattt
     7681 atcgctgtcc gcctgccata cggagtgcaa gctgatgaag cagatgctca aaaagcccta
     7741 gccttcatcc agccagatgt cagcttggtt gtgaatatca aggaatcagc tgatgccatg
     7801 acagctgcag ttgaagcgac aggtagtcct gtttcagcct tcaacaaggg gaatatcaag
     7861 gcacgttgcc gtatgattgc tcagtatgcc cttgctggtt cccatagcgg agcggtcatt
     7921 ggaacagacc acgccgcgga aaatatcaca ggtttcttta ccaagtttgg tgacggcggt
     7981 gcggatattc tccctcttta ccgcctcaat aaacgccaag gaaaacagct cttgcagaaa
     8041 cttggcgcag agccagccct ttatgaaaaa atcccaacgg cagacctaga agaagataaa
     8101 ccaggcctag ctgacgaagt cgcacttgga gtcacctacg cagagattga cgactaccta
     8161 gaaggcaaaa caatcagccc agaagctcaa gcgaccattg aaaactggtg gcacaaaggc
     8221 caacacaaac gccacttacc catcaccgta tttgatgact tttgggagta aaaaggtccg
     8281 ggggaccttt ttagcttctt gccctgaaat taaaaagcaa gaaaaacctc cactggaggt
     8341 tttcagcctc tcatcttgaa ataagaaagt gagagaaggt ctgggggatc ttgaaccccg
     8401 agtttagaaa taagaaaatg aggcagattc agtaactcga agagttcgat ttcatcgtct
     8461 tacccctgca acgatgacta ggtttgaaaa agcttgctag agcgcatttc aaaccaggca
     8521 gcaactgcgt caagaaatta gaagacaaac tcgttttcta gctgttactg agttgagcct
     8581 ttttactacg agtatagaaa taaggaagtg aggtagcatc atgaaatcta tcggtacgca
     8641 aatattacag acagaacgtt tgattttaag aagatttgtg gagagtgatg cagaagccat
     8701 gtttcaaaat tgggcttcat ccgctgagaa tctgacctat gttacctggg atccccatcc
     8761 tgatgtcgaa atcactcgaa actcgatttg caattgggtt gcttcctata ctaatctcaa
     8821 ctattataaa tgggccattt gtctaaaaga aaacccagag caagtaatag gagatatcag
     8881 cattgttaag atagacgagg ctgatttaag ctgtgaaatt ggctatgtgt taggcaaggc
     8941 ttactgggga aatggtatga tgacagagac tttgaaagct atcttggact tttgttttac
     9001 tcaagcaggt tttcaaaagg tcagagcacg ttatgccagt ctcaacccag cttcaggtcg
     9061 tgtcatggaa aaggctggaa tgtcctatct acaaaccatt gttaatggtg tagagagaaa
     9121 aggctatctt gcggatctta tttattatgg tataagtagg gaagaatgtt gaattctatt
     9181 ttctgtttct atcgaagtca actatttatt gtaaatataa taattagcat tccaagttta
     9241 tttgaaactt taaaatagca tattgattag tacaagacag atgttctagt tccttcttta
     9301 atctggttta gtgttagtta aaaaatcgct ttaagcttgt aactaagagg gagctaatcg
     9361 actagattct ccagccgaac aggtggtaat gtacttttta tagtgtaatc ctagctgttg
     9421 ttaaatttaa aatagaatcc tctatcgagt tagggaatta aattcaacca attttattca
     9481 tgttttttct atcaaattat ctaatattaa aatagtctca ttctgatgag aaaact
//
LOCUS       DAVKNL010000072         9188 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVKNL010000072 DAVKNL010000000
VERSION     DAVKNL010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9188
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.072"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(58..291)
                     /locus_tag="VW411_001818"
     CDS             complement(58..291)
                     /locus_tag="VW411_001818"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794499.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
     gene            complement(284..400)
                     /locus_tag="VW411_001819"
     CDS             complement(284..400)
                     /locus_tag="VW411_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU9794500.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            complement(615..965)
                     /locus_tag="VW411_001820"
     CDS             complement(615..965)
                     /locus_tag="VW411_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEU9794501.1"
                     /translation="MLRHPGISPTNDLIAKKIFSNPEITCQFIRDMLDLPAKNVTILE
                     GSDIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQVN
                     QNLEKIRQREGDTH"
     gene            1221..3854
                     /gene="polA"
                     /locus_tag="VW411_001821"
     CDS             1221..3854
                     /gene="polA"
                     /locus_tag="VW411_001821"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEU9794502.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNVAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYDLAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVSESLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFVWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIMVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYVQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDDVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            3939..4376
                     /locus_tag="VW411_001822"
     CDS             3939..4376
                     /locus_tag="VW411_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU9794503.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            4417..4494
                     /locus_tag="VW411_001823"
                     /pseudo
     CDS             4417..4494
                     /locus_tag="VW411_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(4650..5660)
                     /locus_tag="VW411_001824"
     CDS             complement(4650..5660)
                     /locus_tag="VW411_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEU9794504.1"
                     /translation="MSFLSKNGAGILACLLISILSWYLGGFFPVVGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIDAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKELSKFLIVMAMSAIGLKTNLVAMVKSSGKSILLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            5809..6978
                     /locus_tag="VW411_001825"
     CDS             5809..6978
                     /locus_tag="VW411_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9794505.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQAISEIPGVLRLTLGEPDFTT
                     PDHVKEAGKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPATFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            6975..7745
                     /gene="recO"
                     /locus_tag="VW411_001826"
     CDS             6975..7745
                     /gene="recO"
                     /locus_tag="VW411_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEU9794506.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            7742..8734
                     /gene="plsX"
                     /locus_tag="VW411_001827"
     CDS             7742..8734
                     /gene="plsX"
                     /locus_tag="VW411_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEU9794507.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLSGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            8740..8973
                     /locus_tag="VW411_001828"
     CDS             8740..8973
                     /locus_tag="VW411_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU9794508.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            9019..9188
                     /locus_tag="VW411_001829"
                     /pseudo
     CDS             9019..9188
                     /locus_tag="VW411_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2606 a         1736 c         2278 g         2568 t
ORIGIN      
        1 aataggctcc ataatatcca taggggattt acccactatg gatattatgg agccatttta
       61 caacagtgcc tcaaattcag atactgacat acctaattgc tggctggcaa cctcagaagt
      121 cagaagacct tggcgtacta tgtctaggaa ggcaaaaagt ttcctttctg cccttccttc
      181 aactttccca cgctctagtc cttgttcaag gccttcagcc ctagcagttt ccaaggcata
      241 gtcctgtgct aacaagactt gttcttctcg catatgtagt tgactaaaca ttttcctgtc
      301 ctcctcggac cagctcttgt agtccagcag ttgatttgct tgggtaatgg ctcgttgcgg
      361 ttgctgggta aagggcttgt tgccgaaaaa ctccaaccat ggcttgcgaa ccttgtcttt
      421 gctggtttct ctgtattttt taattccaag aatgccatct taaccagatg gttttcctgt
      481 ccattgttgg taatcgccaa tacctcacct gttgtgtctt cgcgcatact aaagctatga
      541 aaagccaggt catctgagaa ataattacta tccacgatag caatggcgta aacaggagcg
      601 atgtgtttgt agctctagtg agtatcacct tctcgctgac gaattttttc aagattttga
      661 ttaacctgac tgcacaggta agcccacaag tgattgatga aaaaattctg atgatggact
      721 tgaatctcaa taattacttg agtaccgtta tccaactccg ccaagacgtc tatactggta
      781 taaaaatcct gcaccgagta aggcatggag agtaatacgt gaatatcgct tccctccaaa
      841 atggtcacat tttttgctgg caagtccagc atatcgcgaa taaattgaca agtgatttct
      901 ggattgctga atattttctt agcaatcaaa tcattagttg ggctgatgcc cggatgtctg
      961 agaatcctcc tattcctcct tctatagtga gttgaaataa gatgtgaata actctatcag
     1021 gaaagtcaaa ttaatttata gaaatatttt agcagtcaag gtgtactatt atagattcaa
     1081 tatactatat tatagcacat ttttaaatct aggtttacta aattctctgg ctctatctat
     1141 ttattcggaa aaagtttgaa aaatacttgc tctagctctt cccaatggta ttttttgatt
     1201 ctttccttta taatgggtgt atggataaga aaaaattatt attgattgat gggtcttctg
     1261 tagcttttcg ggcgtttttt gcgctgtatc agcagttgga ccgttttaag aatgtggctg
     1321 gtttgcatac caatgcgatt tatggttttc agttgatgtt gagtcattta ttggagcggg
     1381 ttgagccgag tcatattttg gtggcttttg atgcgggaaa gacgaccttc cggacagaga
     1441 tgtatgcgga ctataagggt ggtcgggcca agactcctga tgagtttcgt gagcaatttc
     1501 ctttcattcg tgagttgctg gatcatatgg ggattcgtca ctatgatctg gctcagtatg
     1561 aggcggatga catcattggg acgctggata agctagcaga gcaggatggt tttgatatta
     1621 ctattgtcag tggggacaag gatttgattc agctgacgga tgagcatacg gtggttgaaa
     1681 tttccaagaa aggtgtggct gagtttgagg cctttacgcc agattacctc atggaagaaa
     1741 tgggcctcac accagctcag tttatcgatc tcaaggcgct catgggtgat aagtcggata
     1801 atatccctgg ggtgaccaaa gtcggtgaaa agacgggtat taagctcttg ctggagcatg
     1861 gttcgcttga ggggatttat gaaaatattg atggaatgaa gacttctaag atgaaggaaa
     1921 atctcatcaa tgacaaggaa caggcctttt tgtctaaaac actagcgacc attgatacca
     1981 aggcaccgat tgcgattggt ttagaggact tggtctatag tggtccagat gttgaaaatc
     2041 ttgggaaatt ctacgatgag atgggcttca aacagctaaa gcaggcttta aatgtgtcgt
     2101 cagctgatgt gtctgagagt ttggatttta ctattgttga ccaaatcagt caagatatgc
     2161 tgagtgaaga gtctatcttc cactttgagc tttttggtga gaattaccat acggataatt
     2221 tggttggatt tgtctggtct tgtggggata agctctatgc cacagacaag cttgagctgt
     2281 tgcaagaccc gattttcaag gatttcttag aaaaaacatc tctgagagtt tatgacttta
     2341 agaaggttaa agttcttttg caacgttttg gtgtggattt gcaggcgcct gcttttgaca
     2401 tccgtttggc taaatacctc ctttcgactg tggaggacaa tgaaattgcg accatcgcta
     2461 gtctttatgg tcagacttac ttggttgatg atgaaacttt ctacggtaag ggtgttaaaa
     2521 aggccattcc tgaacgtgag aaattcttgg aacacttagc ttgtaaactt gctgttttgg
     2581 tagaaacaga gcctatttta cttgaaaaac tcagcgaaaa tgggcaatta gagcttcttt
     2641 atgatatgga gcaacctctg gcttttgtcc ttgccaagat ggaaattgct gggattatgg
     2701 tcaagaaaga gaccttgctt gagatgcagg ctgaaaatga gcttgtcatt gaaaaactga
     2761 ctcaagagat ttacgagctg gctggtgagg agtttaatgt caactcgcct aagcagttgg
     2821 gcgtgcttct ctttgagaaa ttgggacttc ctctagaata cactaagaaa accaagacag
     2881 gttattcgac agcagtggat gttttagagc gtctcgctcc tattgctccg attgttaaga
     2941 aaatcctgga ttaccgtcaa attgctaaga ttcaatctac ttatgtaatt ggcttgcagg
     3001 actggatttt ggctgatgga aagattcata ctcgctatgt gcaggatttg acccagaccg
     3061 ggcgtttgtc tagtgtggat ccaaacttgc aaaatattcc tgcccgattg gaacaggggc
     3121 gcttgattcg gaaggctttt gtgccagagt gggaggatag tgtgctactc agctctgact
     3181 attcacagat tgaattgcgc gttttggcgc atatttctaa ggatgagcac ttgattaagg
     3241 ccttccaaga gggggcagat atccatactt cgacagccat gcgggtcttt ggcattgagc
     3301 gtcctgatga tgtgactgca aacgaccgtc gcaatgccaa ggcagttaac tttggagtgg
     3361 tttatgggat ttcagacttt ggcttgtcta ataatttggg aattagtcgt aaggaagcca
     3421 aagcctacat tgatacctac tttgaacgtt ttccaggtat taaaaactac atggatgaag
     3481 tggtgcggga ggcgcgtgat aagggctatg tagagaccct ctttaagcgt cgccgtgagt
     3541 tgccagatat caattcgcgc aacttcaata ttcgtggttt tgcggagcga actgctatca
     3601 actcacctat ccagggttcg gcagcagata ttctcaagat tgccatgatt cagctggata
     3661 aagccttggt tgcaggtggt tatcagacta agatgctgtt acaagtgcac gatgaaatcg
     3721 tccttgaagt gcctaaatct gaattggtag agatgaaaaa attggtgaaa caaaccatgg
     3781 aagaagccat tcaactcagt gttcctctta tcgcagatga gaatgaaggg gcaacctggt
     3841 acgaggctaa ataaaaaggg ggctagtcct ccttttttgt agtataattc tgcaaacctt
     3901 ttcaaaatgt gctatactga tgaaaaagga ggatttctat gagtcaagaa tttatcaatc
     3961 caagtgatgg cgtgattcgt cagtatctcg caacgagtaa aacccttgct gtggtgggtt
     4021 tgtctgaccg tgaagaaaca accagcaatc gagtaacaaa ggaaatgcag gctcggggct
     4081 ataaaatcat cccagtcaat cccaaggcgg caggtggcga aatcttgggt gaaaaggctt
     4141 atgctagcct cgctgaaatt ccttttcctg tagatattgt caatgtttat cgtcgcagtg
     4201 aatttctgcc cgatgtggcg cgtgattttc tcaaagctga tgccaagatt ttttgggcac
     4261 agctaggact tgaaagttta gaagcgaaag agattttgcg tgatggtgga tgtgatgata
     4321 tcgtgatgaa tcgctgtatc aagagagaac atactcgctt gattgaggaa gcataagaaa
     4381 aaggtagctg atgggctacc ttttgtgtta tactcaatga aaatcaaaga gcaaactagg
     4441 aaactagcca cgggctgctc aaaacactgt tttgaggttg cagatagaac tgacgaagtc
     4501 agctcaaaac accgttttga ggttgcagat agaactgacg aagtcagctc aaacaccgtt
     4561 ttgaggttgc agatagaact gacgaagtca gtaacatata cctacggcaa ggcgacgttg
     4621 acgtggtttg aagagatttt cgaagagtat tagaaaatgc cgataagggt ctgcatacca
     4681 agagtggtga ggatgatggc aatccagcag atggctccaa gaagaatgga ttttccactg
     4741 gatttgacca tagcgaccag attggttttg agaccgatag cactcatggc catgacaata
     4801 aggaatttag agagttcttt gagaggagta aagaaactac tggacacacc tagagaggtg
     4861 agtagtgtag tgaggagaga ggcaaggata aagtaaagga taaaaagtgg gaagactttt
     4921 ttcagttgca ggctttgctt gttttcttgt tggcgacttt gccagtagga tagaaagagc
     4981 gtgataggga taatggccaa agtacgtgtg agtttaacaa tggttgcaga ctcgagggta
     5041 ttgctttggt aaagactgtc ccaagcgctg gcggcagccg ttacagagga agtgtcgttg
     5101 accgcagtcc ctgcaaagag ggcgaagcct tcattggata gatgaagcca ggtgccgagg
     5161 gttggaaaga tgagcgcagc caagacattg aagaaaaaga taacggaaat ggcttgggct
     5221 acttcctttt ccttagcatc aataacgggc gctgtcgctg caatggcaga acccccacag
     5281 atagaagaac ctactccaac caaggtagcc agttttgtat ccagggcaaa gaaacgctgg
     5341 aagaggtagg caataatcag agctattgag atagtggaca ggatgacagg gagtgaagat
     5401 tggccaactg cgaagacctg cgagatattg agaccaaaac caagcaagac aacggcatat
     5461 tggagcaact tcttggaact aaaggtcaaa ccagcatcca gttgtttata ggacgagaga
     5521 aagggatgta ggagcatgcc tatgaaaatg gcaaaaacgg gcgcgccaac cacagggaag
     5581 aatcctccta agtaccaaga taggatggaa atgagaaggc aggccaagat acctgctcca
     5641 ttttttgata gaaatgacat aaaaacctcc aaataaaatc tattaccatt atagacctat
     5701 aaacaggaaa agtaaaatag aaagtggaaa gctattctct aatgtatttt tgcggtcggg
     5761 gggcttttgt agtataatag agatacgttt tgaaagtagg aggtatctat ggacttaact
     5821 aagcgcttta ataaacagtt agataaaatt caagtttcgt tgattcgtca gtttgaccag
     5881 gctatttcgg agattcctgg ggtcttgcgt ttgaccttgg gggaacctga ttttacaacg
     5941 ccagaccatg tcaaggaggc gggcaagcga gcgattgatc agaaccaatc ctactataca
     6001 gggatgagtg gtctgctgac tctacgtcag gcagccagtg actttgttaa ggaaaagtac
     6061 caactggact atgctcctga aaatgaaatc ttggttacaa ttggggcgac agaggcttta
     6121 tctgcgactt tgacggctat tttggaagag ggagacaagg tacttttgcc agctcctgct
     6181 tatccaggct atgaaccgat tgttaactta gttggggcag aaattgttga gattgatacg
     6241 actgaaaatg gttttgtctt gactcctgag atgttggaga aggccatttt ggagcagggt
     6301 gataagctca aggcggttat tctcaactat ccagccaatc cgacaggaat tacctacagt
     6361 cgagagcagt tagaggcctt ggcagctgtt ttacgcaagt acgaaatttt tgttgtctgt
     6421 gatgaggttt actcagaatt gacctacaca ggcgaagccc atgtgtctct aggaacgatg
     6481 ttgagagacc aggctattat tatcaatggt ttgtctaaat cgcatgccat gacaggttgg
     6541 cgtttggggc tgattttcgc tcctgcgacc ttcacagccc agttaatcaa gagtcaccag
     6601 tacttggtca ctgccgcaaa taccatggcg caacatgctg cggtagaagc cttgacggct
     6661 ggtaaaaacg atgcggagcc catgaagaag gaatatatcc aacgtcggga ctatatcatc
     6721 gaaaaaatga ctgctcttgg ttttgagatt atcaaaccag acggtgcctt ctatattttt
     6781 gctaaaattc cagcgggcta caatcaagac tcctttgctt ttctgaagga ttttgctcag
     6841 aagaaggccg ttgcctttat ccctggtgca gcctttggac gttacgggga aggctacgtc
     6901 cgcctatctt atgcagccag catggagact atcaaagaag ccatgaaacg acttgaggag
     6961 tacatgagag aagcatgatt cagtctatca cgagtcaagg cttggtgctt tacaatcgca
     7021 attttcgtga ggatgacaag ctcgtcaaaa tttttacaga gcaggttggc aaacgcatgt
     7081 tttttgtcaa acacgctggt cagtctaagc tggcgcctgt tattcagccc ttggtgctgg
     7141 cacgatttct cttgcgaatc aatgatgacg gactcagtta catcgaagac tatcatgagg
     7201 tcatgacttt tcccaagatt aatagtgacc tctttgtcat ggcctatgcg acctatgtgg
     7261 cagctcttgc agatgctagt ttgcaggaca atcagcagga tgctcccttg tttgcttttt
     7321 tgcaaaagac tttggagttg atggaagcag gcttggatta tcaggttttg accaatattt
     7381 ttgaaattca aattttgact cgatttggaa tcagcctcaa ttttaatgag tgtgtcttct
     7441 gccatcgggt tggtcaggct tttgactttt ctttcaaata tggagcctgc ctctgtccag
     7501 agcattatca tgaggataag agacgttgtc atctcaatcc caatatcccc tatctgctca
     7561 atcaatttca agctattgat tttgagactt tggagaccat ttcgctcaag cctggaatca
     7621 agcaagagct acgccaattt atggatcaat tatatgaaga gtacgttggg attcacctaa
     7681 aatcaaagaa atttattgat tccctagcag actggggaca attactaaaa gaggaaaaga
     7741 aatgaaaaaa atcgcagtag atgccatggg gggcgattac gcacctcagg ccattgttga
     7801 gggtgtcaat caagccctat ctgacttttc agatatcgag gttcaacttt acggagatga
     7861 agctaaaatc aagcaatatc tgacagcgac agagcgcgtc agcattatcc atacggatga
     7921 gaagattgat tcggatgatg aacctacgag agctattcgg aataagaaaa atgccagtat
     7981 ggtattggca gccaaggctg tcaaagatgg tgaagcagac gctgtccttt cggctgggaa
     8041 tacaggtgcc ttgttggcag caggattctt catcgtgggt cgtatcaaga atatcgaccg
     8101 tcctggactc atgtctacct tgcctaccgt tgatggaaaa ggttttgaca tgctagacct
     8161 tggtgccaat gcagaaaata cagcccagca cctccatcaa tatgcggttc taggttcctt
     8221 ctatgctaaa aatgtccgtg gcattgcgca accacgcgtt ggtttgctca acaacggaac
     8281 agagagtagc aagggcgacc cgcttcgtaa ggaaacttat gaattactgg cggctgatga
     8341 aagtttgaac tttatcggaa acgtggaagc gcgtgatttg atgaatggcg ttgcagatgt
     8401 tgttgtggca gatggtttca cgggaaacgc tgtgctcaaa tccatcgaag ggacagctat
     8461 gggaatcatg ggcttgctca agacagctat tacaggtggt ggtcttcgag cgaaactagg
     8521 tgccctcctt ctcaaggaca gcctcagtgg tttgaaaaaa cagctcaatt attcagatgt
     8581 tggtggagcg gtcttgtttg gtgttaaggc acctgttgtc aagactcatg gctcaagcga
     8641 tgccaaggct gtttatagta cgattcgtca gatccgtacc atgctagaaa cagacgtggt
     8701 tgcccagact gcgcgtgaat tttcaggaga ataaaagaga tgacagaaaa agaaattttt
     8761 gaccgtattg tgaccattat ccaagagcga cagggagagg actttgtcgt gacagaatcc
     8821 ttgagtctga aagacgattt ggatgcggat tctgttgact tgatggagtt tatcttgact
     8881 ctggaagatg aatttagtat cgaaatcagc gatgaagaaa ttgaccaact ccaaaacgta
     8941 ggagatgtgg ttaaaatcat tcaaggaaaa tagcaatcgg agttccaagt caacggaagt
     9001 agatggtttt tagaaatgag aaatatcgga caagctggta aaatcttggc tgacagtggt
     9061 tatcaagggc tcatgaagat atatcctcaa gcacaaactc cacgtaaatc cagcaaactc
     9121 aagccgctaa cagttgaaga taaagcctgt aatcatgcgc tatctaagga gataagcaag
     9181 gttgagaa
//
LOCUS       DAVKNL010000073         9040 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVKNL010000073 DAVKNL010000000
VERSION     DAVKNL010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9040
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.073"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(330..1178)
                     /gene="dprA"
                     /locus_tag="VW411_001830"
     CDS             complement(330..1178)
                     /gene="dprA"
                     /locus_tag="VW411_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEU9794509.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            complement(1274..1963)
                     /locus_tag="VW411_001831"
     CDS             complement(1274..1963)
                     /locus_tag="VW411_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEU9794510.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYVSGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            complement(1975..2853)
                     /locus_tag="VW411_001832"
     CDS             complement(1975..2853)
                     /locus_tag="VW411_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU9794511.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAATHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(2843..3712)
                     /gene="licA"
                     /locus_tag="VW411_001833"
     CDS             complement(2843..3712)
                     /gene="licA"
                     /locus_tag="VW411_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEU9794512.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKITPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     IKGLASYGGSDEK"
     gene            complement(3729..4751)
                     /locus_tag="VW411_001834"
     CDS             complement(3729..4751)
                     /locus_tag="VW411_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU9794513.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPEAEIVVIGRHWEKLELFSFAKECYITDNI
                     PEDLAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFENAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(4756..5463)
                     /locus_tag="VW411_001835"
     CDS             complement(4756..5463)
                     /locus_tag="VW411_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEU9794514.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKNIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            5799..7286
                     /gene="tacF"
                     /locus_tag="VW411_001836"
     CDS             5799..7286
                     /gene="tacF"
                     /locus_tag="VW411_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEU9794515.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVAPEYYLLTTML
                     SETSLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            7296..8099
                     /locus_tag="VW411_001837"
     CDS             7296..8099
                     /locus_tag="VW411_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9794516.1"
                     /translation="MKQLTVEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            8101..8910
                     /locus_tag="VW411_001838"
     CDS             8101..8910
                     /locus_tag="VW411_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9794517.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
BASE COUNT         2851 a         1827 c         1519 g         2843 t
ORIGIN      
        1 cactatactt tcccttatac tcaatgaaaa tcaaagcgca aactaggaag ctagccacag
       61 gttgctcaaa gcactgcttt gaggttgtag ataagactga cgaagtcagc tcaaaatact
      121 gttttgaggt tgcagatgga agctgacgta gtttgaagag attttcgaag agtattatat
      181 gagaaaaatc ctctactcta ctaatccact tagaaaaaac gcatattatc aagtgttaaa
      241 aacttgctaa tatgcgtttt attatggaaa tgttgggttc attttctctt aaaattgatt
      301 ttttcttaga attctagctt aggtcatttt taaaattcaa attccgcaag aacatcttgc
      361 ccactggtga ccaattttgc tccttcttga atcaaatgat ggcaaccgtc tgatagtcca
      421 tctaaaatgc taccaggaat agcaaagaca tcgcgtcctt cttccattgc tcgctcacac
      481 gtaatgagac tacctgaacg catcttagcc tctgctacaa tcacaccacg acaaagtcca
      541 gcaatgatgc gattacgggc aggaaaatga aatttcagag gttgttcacc aggtccatat
      601 tcacttagaa ccagatggtc attgccgatg tagtcttgca agcgtttatt ggctttagga
      661 taaaacacat ccagtcctgt tccaatcact gcaatggttt ttccgccatt ctgaagagct
      721 gccatatgag ctgctgtgtc aatgcccttg gccagaccac tgacaataac cagttcattt
      781 tccaagcctt gaatgacttt ttcaactgac ttagctccct gtttgctaca agcacgactg
      841 cccacgaccg ctaccttcgg gaatttcagg aggtcaagat ttcccttgta aaataaaagt
      901 acaggcgcat catatatttc actcaaatcc caaggataac agtcatctaa aatagagaaa
      961 gatggaaatt tttgaaactc tttcgacaaa tgcgcatcgt ctatctgaaa ataacgttcc
     1021 ataaaaacgg ctggattacg gcaacctgag atatctgcaa tatcacccaa caaaagctcc
     1081 tgatcaacat tttcaccgta ttctagcact ttcaaaatct gttgattggt caaacctgat
     1141 ttttttaact tatagatttc atagtttgtg attttcataa ataactccat ttctttttct
     1201 actcatctat ctattcgtaa aagttcgtaa aaaatcaaaa aacatccgac tattttgtca
     1261 gatgttggaa tctttaattt tcgtttttaa gaatttcttc taatttacga tagtcttgga
     1321 cactatcgat ctcataaatg ctattgcctt ctaattcttc aacatagaca tctagctctt
     1381 tgatattatc cttaaccata ttgtcccaat agagatcaac aaattcacca cttacataag
     1441 ccttgtcgat aaagctgaca atcttttctg cagttggagc atcccagaag gatacaccac
     1501 taaggatgcg acctgccttg ctatcaacaa taatgtcttg aaccttgtag tcatctccat
     1561 aaaccaagaa ccattcgttg gtacaatctt cacgataaac actaaaataa gtcgaacgtg
     1621 tcaaatcatt gcggaacata tttttaaaga gataattgtc agcatcaata acatagctgt
     1681 tggccaattc ttcttttaca agatagagag agtaaaagtt attgtagtca gcgtatttat
     1741 cattgaaaac gagacgaaca ccgtatttct ctttcaagta atcgaattgt tctttaagat
     1801 aaccaacaat gatgatgatg tcattgattc ctttttcttt gagaaactca atttggtact
     1861 caatcaaagg tttttgatta acctgaacca aggctttagg ggtattttca gtcataggac
     1921 gcaagcgagt tcccaatccc gctgctaaga taatggcttt cacacgaatc tcctttattc
     1981 tttaataata atataaactc cagcaatgac gacaagtgac gtcataatgg tcagcatatc
     2041 tagcggtgca cccaagaaaa caactgcaaa caagaccgtc catactacat agctcacgtt
     2101 caagcctgta gccttggctg gttgcaagcg attgatagcg atataataag ccaagtagga
     2161 aatcatatca aaggctgcaa aaacaatcat gagacctagc aattgtccat tggctactgc
     2221 agtaaatgac tgatgagaga agagcacaat cacaagatag gacaagaacg aagttacttg
     2281 acggattaag agggcttcga tttcactcaa ttcactttcc atggcaaaag agctaagaac
     2341 actctcactt ccccatgcaa tagcacaaac caaagcacaa agaatcccaa tgtagaaaga
     2401 attaacctgt tcaaccttat aggtctgagc aataatccct ccaataatca agacaatccc
     2461 aaatacagta tttttcgaaa tcttgtgctt caaaaagaag aaagccaata gaactgaaat
     2521 cgcagggtaa atagccgata cagatgaagc taaagaactt ccgatatact taactgcata
     2581 aagattggcc tgcataccga tagggcctgc tagcaaggct ccgatgataa cactgacatt
     2641 gcgaatattt aagaaaattg agaggcgaac tttcccttct tttaccaaga gaaaagctag
     2701 taagataaag atgctcaaaa aatcatgagt tgcagccacc acaaagggtg acaaatctgt
     2761 aaaaatcgaa aagatataag cactaaccgt tagacccaag ccccagaaaa tacctgagag
     2821 aaggccaaaa ggaactccgt ttttactttt catctgaacc tccataagaa gccaaacctt
     2881 taatagctct ttggtaacga ttcacaccat agtcaccaaa atcttcacct tgctcttcct
     2941 tatagacagt ccatagactc caaatagtat cttgtaaaat tttataaata gcaatctttt
     3001 catgagaaac cggtgtttgg tcactctcat agtgagataa gaaagtttct tcctcttggg
     3061 aagtgaattc agactctaaa aagagggcag ccaaatccca cattggatca ttcattgatg
     3121 aatattccca gtcaatcaaa taaagtcgtc cttgaggtga ttcgataaag ttttcaggca
     3181 ccaaatcgat atgacaagat tttctgtcaa cacctaagtc agccagtctt ttctctaagg
     3241 agaagactgc atttctaaca gattcatagt tggcataagg aatttgttct tcaatcaagg
     3301 attcgtattt tttgatttct tcaaaaggag caaattctcc tcttaattcc ttagcagacg
     3361 tatgaatagt ttgtaatatt ggagtaattt tgtcgaactt ggtcttgatt gacgttgaat
     3421 caagcgtaat cgcagattcg atatactcat ttactttgat accagcttca atatcaaaaa
     3481 gataattttt tacatctaag cctaaatcct ttagtagttc aagattgtac ttttcatctt
     3541 gtcgattgat aagcttttct gtccctttac caaagaattt aacaatgtat tgcttatttg
     3601 ttgttttggc caaatagttt tgattggtca ttccacctag ttgttcaaca ctgaggactt
     3661 cctcttcttg actaagtaag gaagaaattt tttctttaat gattttctcc acaattaacc
     3721 tccagtactt atacttccca cttaaacact gttttaaagg ctgtgtttaa atcggttgca
     3781 aagacacgat gaatatcttt aatttctctt acaggttctt ctagataaag gatattttta
     3841 agacgattgg caaatttctt gacttccatc atttggatag cattttcaaa atcaatgcga
     3901 ccagaacgag atgacccaac caaaatcaag cccttttcta aggcatcgcg agtattgaga
     3961 ttgactttat attcgctaac tcccatcatg agaatcgttc cctgaggacg aatgtagcga
     4021 atcaagtcat taatagctgg tccagtacca tcaccaccac aacattcaaa agcatggtca
     4081 aaggccaaat cttcaggaat attatccgta atatagcatt ctttggcaaa tgagaagagt
     4141 tccaactttt cccaatgacg accaataacc acaatctctg cttctggcaa agtatagttg
     4201 ataatattgg caaccacaaa agctaaactt ccatctccaa taacggcgat ccgctcccgc
     4261 ttgctatgag caagagtcaa tagacgattc atagcgtgca tgcccacact gacaaactct
     4321 gtaatggctg caaccgtatc ttcaatagca tcataagcca ccacacgatc tttagggaga
     4381 gaaacaaact ctctcataaa gccatcaaat ccactagaca agaaatgggt ccctgtcatg
     4441 tagttttcat agaattcttc atcactctgc ataggagact gattgggaat catgacaact
     4501 ttttgaccaa cctcgtaggt tccggtcggg tcagaaatga cggttccaca tgactcgtga
     4561 atcattgcca ttggaagctt tttattcaaa atcttgggat cacgttttcc ctgatagtaa
     4621 cgctgatccg catgacagac agccatgtag ttgggacgga taaggatatg attctcttgg
     4681 tcaatagcct cttcctgata tttgacattg ataaacttag gcttagttag ttgataaatt
     4741 tgattaatca ttttactagt ctttctcaat catacttttt gcaatcttca aatctgttac
     4801 ggttgtaatc ttcagatttg agtattcacc tttggccaaa gccacatctt ttcctttgat
     4861 cacaaagatt ttacatgcat ctgtcaagat ttccttctct tcatcagaaa gagatccata
     4921 aaggtccatg aagtccttgc aacggaatgt ttgaggtgtt tgtccttgat aaaggtgagc
     4981 acgatttgga atatctgtaa taaattgacc attggtactt tcaacgatag tatcaaccgc
     5041 ttctaccact gtgtccactg cgtcatgatt ttgggcaagt tggatattgt cctgaatcat
     5101 gcgaagtgta ataaatggac gaacagaatc gtgggtaaca acgatatcct ctggagtaag
     5161 cggacgataa gcatcaatgg cttcaatgat gttcttaata cttgtattgc ggtcagcacc
     5221 accctttgta atgatgatac gttccttata aagaggaaga tatttatcta caagatcttc
     5281 tgcatgagaa acccagtctc catgaacacc aactacaatt ttttcaatac ttggctccaa
     5341 gacaaatttt tcaattgtat gaatcaaaat aggtcgatca cctagctcta aaaattgttt
     5401 tggcaagtta ctgatcccca tgcgtgtgcc agttccaccg gcaagaattc ctgcataaat
     5461 catctatttt ctcctttgtc ttactaaaaa aacttattct ctctattata ccacaatcta
     5521 gtcctatcat aaaagaaata ctggtcctcc ccttctagaa taggagaatt atgcagttct
     5581 attattcaaa gaaactagtc catttctagt aatgactagg aattctgtag caattactaa
     5641 taaatggctg cgaaatttta gagtccttca gaataatata ctttccttaa agaaaactta
     5701 aaacttaaga tttgaaactt caaagtgatt acttgactta ctgcccttca tttcttatta
     5761 gctaacttta tgatataatg aaaaacgagg taaattgaat gaaaagtata aaattaaatg
     5821 ctctatctta catgggaatt cgtgtcttga atattatttt tcccatccta actggaacct
     5881 atgtcgcgcg tgtcttggac cgaactgact atggttactt caactcagtc gacactattt
     5941 tgtcattttt cttgcccttt gcaacttatg gtgtctataa ctacggttta agggctatca
     6001 gtaatgtcaa ggataacaaa aaagatctta acagaacctt ttctagtctt ttttatttgt
     6061 gcatcgcttg tacgattttg accactgctg tctatatcct agcctatcct ctcttcttta
     6121 ctgataatcc aatcgtcaaa aaggtctacc ttgttatggg gattcaactc attgcccaga
     6181 ttttttcaat cgaatgggtc aatgaagctc tggaaaatta cagttttctc ttttacaaaa
     6241 ctgccttcat ccgtatcctg atgctggtct ctattttctt atttgttaaa aatgaacacg
     6301 atattgttgt ctatacactt gtgatgagtt tatcgacgct gattaactac ctgattagtt
     6361 atttttggat taaaagagac atcaaacttg ttaaaattca cctaagtgat tttaaaccac
     6421 tctttctccc tctgacagcc atgttagtct ttgccaatgc caatatgctc ttcacttttt
     6481 tagatcgcct cttcctcgtt aaaacaggga ttgatgtcaa cgttagttac tataccatag
     6541 ctcagcgaat tgtgaccgtt atagctgggg ttgtaacagg tgcaattgga gtgagtgtgc
     6601 ctcgtctcag ttactatctg gggaaaggag acaaagaagc ctatgtttct ctggttaata
     6661 gaggtagtcg aatctttaac ttctttatca ttccactgag ttttggactc atggttttag
     6721 gaccaaatgc catcctactt tacggtagtg aaaaatatat cggaggcggc atcttgacct
     6781 ctctcttcgc ttttcgtacg attatcctgg ccttagatac cattcttggt tcccaaattc
     6841 tctttaccaa tggctatgaa aaacgtatca cagtctatac agtctttgct gggctactca
     6901 atttgggctt gaatagtctc ctttttttca accatatcgt ggctcctgaa tactacttac
     6961 tgacaactat gctatcagag acttctctac ttgttttcta tatcattttc atccatagaa
     7021 aacaactcat ccacttggga catatcttta gctatactgt tcgatactct ctcttttcac
     7081 tttcctttgt agcaatttat ttcctgatta atttcgtgta tcctgtagat atggtcatta
     7141 atttgccatt tttgattaat actggtttga ttgtcttgct atcagctatc tcttatatta
     7201 gtctacttgt cttcacaaaa gatagcattt tctatgaatt tttaaaccat gtcctagcct
     7261 taaaaaataa atttaaaaaa tcataggagt ttaaaatgaa acaactaacc gttgaagatg
     7321 ccaaacaaat tgaattagaa attttggatt atattgatac tctctgtaaa aagcacaata
     7381 tcaactatat tattaactac ggtactctga ttggggcggt tcgacatgag ggctttatcc
     7441 cttgggacga cgatattgat ctgtccatgc ctagagaaga ctaccaacga tttattaaca
     7501 tttttcaaaa ggaaaaaagc aagtataagc tcctatcctt agaaactgat aagaactact
     7561 ttaacaactt tatcaagata accgacagta cgactaaaat tattgatact cgaaatacaa
     7621 aaacctatga gtctggtatc tttatcgata ttttccctat agatcgcttt gatgatccta
     7681 aggtcattga tacttgttat aaactggaaa gcttcaaact gctgtctttc agtaaacata
     7741 aaaatattgt ctataaggat agccttttaa aagattggat acgaacagcc ttctggttac
     7801 tccttcgacc ggtttctcct cgttattttg caaataaaat cgagaaagaa attcaaaaat
     7861 atagtcgtga aaatgggcaa tatatggctt ttatcccttc aaaatttaag gaaaaggaag
     7921 tcttcccaag tggtaccttt gataaaacaa tcgatttacc ctttgagaat ttaagccttc
     7981 ctgcacctga aaaatttgat actattttga cacaatttta tggagattat atgaccctac
     8041 caccagaaga aaaacgcttc tacagtcatg aatttcacgc ttataaattg gaggattagg
     8101 atgcaatatt tagaaaaaaa agaaattaaa gaaattcaac tagccctgct ggactatatt
     8161 gatgagactt gtaagaaaca tgatattcct tattttctca gttatggaac catgcttgga
     8221 gccatccgcc acaaaggtat gattccttgg gatgatgata ttgatatttc cctttatcgt
     8281 gaggattatg agcgtttact gaagattatt gaagaagaaa atcaccctcg ctacaaggtt
     8341 ctttcctacg atacatcttc ttggtacttc cataatttcg catcgatttt ggacacttct
     8401 actgttatag aagaccatgt taagtacaag cgtcatgata ccagcctttt catcgatgtc
     8461 ttcccaattg atcgatttac agacttgagc attgtcgaca agagctataa gtatgtggct
     8521 cttcgtcaac tagcttatat caaaaaatca cgagcagttc acggtgatag caaactaaaa
     8581 gattttctta gattatgtag ctggtacgct ctccgatttg tcaatcctcg ctacttttac
     8641 aagaaaattg atcaactagt caaaaatgct gtaaccaaca ctcctcaata tgaaggagga
     8701 gttgggatcg gtaaggaagg gatgaaagaa atcttcccag ttgatacctt taaagaactg
     8761 attttaactg agtttgaggg ccgtatgttg cctgttccca aaaaatatga ccaattttta
     8821 acccagatgt atggcgatta tatgacacca ccatcaaaag aaatgcaaga gtggtatagt
     8881 catagcatta aagcttatcg caaaaactga ttgaggggga ttatacaaac tactaagata
     8941 gaggttattc aaaaacataa ttttagtaga aaatgaaata catattccca caataaaacg
     9001 catcatatca aggtttttga aaaaccttga tatgatgcgt
//
LOCUS       DAVKNL010000074         8646 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVKNL010000074 DAVKNL010000000
VERSION     DAVKNL010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8646
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.074"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            <1..416
                     /locus_tag="VW411_001839"
     CDS             <1..416
                     /locus_tag="VW411_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HEU9794518.1"
                     /translation="RIISVIKISTDIERIGDRIVEILKNLQILQNNEILKKIISKIKI
                     LHEVIGLHMNRAISCYREEQSGCLDMVVIQKQNEIEELSINMEKKIMNYIFEDDGNVS
                     EVISALDIIHHLDKIAHTTQAIYKWIMYRKYGNIN"
     gene            429..1904
                     /gene="galT"
                     /locus_tag="VW411_001840"
     CDS             429..1904
                     /gene="galT"
                     /locus_tag="VW411_001840"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEU9794519.1"
                     /translation="MKTIIDNFVDRVIEYGMYNEIDKIYVKNRVLALIGEEGIDRISD
                     ENDLKQIKDYLVEIALKNGKIKDLIEEKECLGAELMNFIVPLPSRLNDIFWSSYDISP
                     QEAVEEFYKLSKDSDYIKTSAIAKNIEFRASTKYGELEITINLSKPEKDPKTIAAEKL
                     VKATNYPKCLLCMENEGYQGRINYPARSNHRIIRLKLGDEVWGFQYSPYSYFNEHAIF
                     LNSQHVPMAITSKTFEQLLEIVDILPGYFAGSNSDLPISGGSILSHNHYQGGKHIFPM
                     EKAKFESEFCFKDFEDVNAGIVKWPMSVIRLQSENKNRLLDLATKILNKWREYSDLEV
                     DIIAMTEDVPHHTVTPIARKVDGRYELDIVLRDNHTTEQYPDGVFHPHQDVQHIKKEN
                     IGLIEVMGLAILPPRLKPELEEVGKYLLGEDNAIADYHLEWADQLKEKYPRINKEEVN
                     SVVQHEAGQVFARVLEDAGVYKNTPSGHEAFMRFVKSVGIN"
     gene            1917..2927
                     /gene="galE"
                     /locus_tag="VW411_001841"
     CDS             1917..2927
                     /gene="galE"
                     /locus_tag="VW411_001841"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9794520.1"
                     /translation="MAILVTGGAGYIGSHTVVELLNLGKEVIIVDNLSNSSILVLDRI
                     EAITGIRPVFYELDVCDKQALRKVFEQESIDAAIHFAGYKAVGESVQKPVMYYKNNIM
                     STLALVEVMSEFNVKKIVFSSSATVYGINNQSPLIETMQTSATNPYGYTKVMLEQILK
                     DVHVADSEWSIALLRYFNPIGAHESGLIGEDPSGIPNNLMPYIAQVAVGKLSELSVFG
                     NDYDTLDGTGVRDYIHVVDLAIGHIKALEKVSEKTDVYIYNLGSGEGTSVLQLVNTFE
                     SVNKIPIPYKIVPRRSGDVATCYANADKAYKELNWRTTKSIEDMCRDTWNWQSKNPNG
                     YN"
     gene            3107..3766
                     /locus_tag="VW411_001842"
     CDS             3107..3766
                     /locus_tag="VW411_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEU9794521.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            3791..4858
                     /locus_tag="VW411_001843"
     CDS             3791..4858
                     /locus_tag="VW411_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9794522.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSISGLQGGTGRAILASILVRYLDDKGELGVSEKGWEVAKEYLKNAYTL
                     QKGESSIVKMLDKEDPIQYGMMWGSGALVGQKEQNVVFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKNFGSIPANKDALKDLPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVQ"
     gene            4961..5971
                     /locus_tag="VW411_001844"
     CDS             4961..5971
                     /locus_tag="VW411_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9794523.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            5983..7674
                     /locus_tag="VW411_001845"
     CDS             5983..7674
                     /locus_tag="VW411_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9794524.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDPNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            7677..8387
                     /locus_tag="VW411_001846"
     CDS             7677..8387
                     /locus_tag="VW411_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEU9794525.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         2942 a         1175 c         1693 g         2836 t
ORIGIN      
        1 gaagaattat tagtgtaata aaaatatcaa ctgacattga acgtattggg gatagaattg
       61 ttgaaatttt aaaaaatcta cagattcttc aaaataatga aattttgaag aaaattattt
      121 caaaaataaa aatacttcat gaagttatcg gactgcatat gaatagggct atatcttgtt
      181 atagagagga acaatctgga tgtttagata tggttgttat acaaaaacag aatgagatag
      241 aagagcttag tataaatatg gaaaagaaaa taatgaatta catatttgaa gatgatggga
      301 acgtttctga agtaattagt gctttagata ttattcatca ccttgataaa attgctcata
      361 ctacgcaagc aatttataaa tggataatgt atagaaaata tgggaacata aattagaaag
      421 aaaaatatat gaaaacaatt attgataatt ttgttgatag agtaattgaa tacggaatgt
      481 ataatgaaat tgacaaaata tatgtgaaaa atagagtact agcgttaata ggtgaggagg
      541 gaattgatcg catttccgat gaaaatgacc tgaaacaaat aaaagattac ttagtggaaa
      601 tcgccttaaa aaacggtaaa attaaagatt taatcgagga aaaggaatgt ttaggagcag
      661 agttaatgaa tttcatagtt cctttaccta gtcgattaaa tgatattttt tggagttcat
      721 atgatatatc acctcaagaa gcagttgaag agttttataa gttaagtaaa gatagtgatt
      781 atattaaaac ttctgctatt gctaagaata ttgagtttcg agcatcaact aaatacggag
      841 agctagagat tacgataaat ttgtcaaaac ccgaaaaaga tccaaaaaca attgctgctg
      901 aaaaattagt aaaagcaact aattatccca aatgtttact atgtatggaa aatgaaggat
      961 atcaggggcg aataaattat ccagcacgtt cgaatcatag aattattcgg ttgaaacttg
     1021 gtgacgaagt atggggattc cagtattctc cctactcata ctttaatgag catgcgatat
     1081 ttttgaatag tcaacatgta ccaatggcta ttacctctaa aacatttgaa caattattgg
     1141 agattgttga tattttgcca ggatattttg ctggttcaaa ttctgatctt cctatctcag
     1201 gaggctccat tcttagtcat aaccactacc agggagggaa acatattttc ccaatggaaa
     1261 aagcaaaatt tgagagtgaa ttttgtttta aagactttga ggatgttaat gctggtattg
     1321 taaaatggcc aatgtcagta attaggttac aaagtgaaaa taaaaatcgc ttgttagatt
     1381 tagctactaa aattctaaat aagtggagag aatattcaga tttagaagta gacatcattg
     1441 ctatgactga agatgtccca caccatacgg tgactcctat tgctcgtaaa gtggacggga
     1501 gatacgaatt agatattgtt ctaagagata atcatactac tgaacaatac ccagatggtg
     1561 ttttccatcc tcatcaagat gtacaacata ttaaaaagga aaatatcggg ttaatcgaag
     1621 tgatgggact tgcgatatta ccacctcgtt tgaaaccaga gttagaagaa gttggaaaat
     1681 atcttttagg agaggataat gcaattgcgg attatcattt agaatgggcc gaccaattga
     1741 aagaaaaata tcctcgcatt aataaagaag aggttaatag cgtagttcaa cacgaagcag
     1801 gacaagtgtt tgcgagagtt ctggaagatg caggagttta taagaataca ccgtctgggc
     1861 atgaagcgtt tatgagattt gttaagtcgg ttggaattaa ttaggaggaa acccagatgg
     1921 caatattggt aacaggcgga gctggttata ttggtagcca taccgtagta gaattactaa
     1981 atttaggaaa ggaagttatc attgtcgaca acctttcgaa ctctagcatc ttggtgctag
     2041 accgtattga agcaattaca ggaatacgtc ccgtgtttta cgaattagat gtttgtgata
     2101 aacaagcatt gagaaaggta tttgaacaag aatcgattga tgctgcaatt cattttgcag
     2161 gttataaagc tgtcggagaa tccgtgcaaa agcctgtgat gtactacaaa aataatatta
     2221 tgagtacatt ggcacttgtt gaagtgatgt cagaatttaa tgttaaaaag attgtctttt
     2281 cttcaagtgc gactgtatat ggaattaaca atcagtcacc tctaattgag acgatgcaaa
     2341 caagtgcgac aaatccttat gggtatacga aagtgatgct tgagcaaatt ttaaaagatg
     2401 ttcatgtggc agattcagaa tggagtattg cgttgcttcg ttatttcaat ccaattggtg
     2461 ctcatgagtc tggtttgatt ggggaagatc cctcaggaat tcctaacaac ttgatgcctt
     2521 atattgcaca agtagcggta ggtaagctat cagagctaag tgtgttcgga aatgattatg
     2581 atacgcttga tggtactggt gtgcgtgatt atatccatgt agtagattta gcaataggac
     2641 atataaaagc tttagaaaaa gtatctgaaa aaacagatgt ttatatctat aacctaggct
     2701 cgggagaagg cactagtgta ttacaacttg taaatacatt tgaaagtgtt aataagatcc
     2761 ctattcctta taaaatagtt ccaagacgtt caggagacgt tgcaacttgt tatgcaaatg
     2821 cagacaaagc atataaggaa ttaaattgga ggacaacaaa atcgattgaa gacatgtgta
     2881 gagatacatg gaattggcaa tcaaaaaatc ccaatggcta taattgatga tactgtctat
     2941 atgagtgctg tattttgaaa aaattactta accttttaat gaaatgcata tttaatgaaa
     3001 gataaattta aaaaaatgtt tgcaagtgct tgacaaagga aacggtttca ttgtataatg
     3061 taaacatgat gacaccgatg tcaaatataa aaaagaaggt ctttatatga aaaacttaac
     3121 aaaaattaag ttcaaagaga atggggaatt taatcatttc cctgggaata cagttgtagc
     3181 aaatctttat actaaacaag atttgatgga agttgttgat attattcaat cacgttatag
     3241 agaattacca tttatcgata agtttacttt aactccaagg aattctattc acatgactgt
     3301 aattgaattg ttgtgccatg aaaatcgtga aacggagttt tggagcagta atcttcctct
     3361 agatacacct ttacaggaaa tacatgatta ctttgctaaa caacttgaaa tttttccatt
     3421 gttggatgaa gaaattcata tgcgtataac tgaaatggga aaacaaaaca tactagttga
     3481 acctgcagat gaagcttctg caaagagatt agaagaaatt cgtacttatg tctcagaaaa
     3541 agcaggtgtt tgtttcccta atcatgatag atatcaattc catatttcaa ttgggtatct
     3601 tcggattcct ctaaccgaag aggaggaaga agagtttact aaagtcagag cagaattaac
     3661 tgaaatttta ttagagaaga ttccaactat tactgtaaac cgtattgact atactgtatt
     3721 tgaagatatg agacaatttg ttccatatca cgaaaaattt aaataaaata ataaaaaagg
     3781 agaacttatc atgaaaaaaa tgaaagtttg gtctactgta cttgcaacgg gagttgctct
     3841 tactacactt gctgcttgct ctggaggttc aaattctacg actgcttctt catctgaaga
     3901 aaaagctgat aaaagtcaag aattagttat ctattcgaac tcagtctcaa atggtcgtgg
     3961 tgattggtta actgctaaag caaaagaagc tggttttaat ataaaaatgg ttgatatcgc
     4021 tggcgctcaa ttagcagacc gtgttattgc tgagaagaat aatgcagttg cagatatggt
     4081 atttggaatt ggtgctgttg attcaaataa aattagagat caaaaattac tagtacagta
     4141 caagcctaaa tggttagata aaattgatca atctttatca gataaagata attattataa
     4201 tcctgtgatt gttcaaccat tagttttaat tggggcgcct gatgtaaaag aaatgcctaa
     4261 agattggact gaattaggta gtaagtataa aggtaaatat tcaatttctg gtcttcaagg
     4321 aggtacagga cgggcaattc tagcaagtat cttagttcga taccttgatg ataaaggtga
     4381 attaggtgtt tccgaaaaag gttgggaagt agcaaaagaa tatttgaaaa atgcatacac
     4441 tcttcaaaag ggagaaagtt caattgttaa gatgttagac aaagaagatc caatacaata
     4501 tggaatgatg tggggttctg gtgcattagt tggacaaaaa gaacaaaatg ttgttttcaa
     4561 agttatgact cctgagattg gtgtaccatt tgtaactgaa caaactatgg ttttaagcac
     4621 tagtaaaaaa caagcgttag ctaaagaatt tattgattgg tttggtcaat cagaaattca
     4681 agtagaatat agtaagaact ttggatctat tcctgcaaat aaagatgccc tcaaagatct
     4741 acctgaagat acgaagaaat ttgttgatca agtgaaacca caaaatattg actgggaagc
     4801 tgttggaaag catttggatg aatgggtaga aaaagctgaa ttagaatacg tacaataaga
     4861 aataagagtt aatttttatg tccaagtctc tctattattt ctaaatttat ggaagaaata
     4921 aagagaggct tttggtttag taatagaggg agttagtgag atgattaaat ttgataatat
     4981 tcaaattaaa tatggtgatt ttgttgcaat tgataatctg aatttagata tacatgaagg
     5041 ggaatttttt acatttcttg ggccttcagg atgtggtaaa tcaactactt tgagagcatt
     5101 ggtaggtttt ctagatccat catcaggaag tattgaagtt aatggaacag atgtcactca
     5161 tttggaacct gaaaagcgtg gaattggtat tgtatttcaa tcttatgcgc tatttccaac
     5221 tatgactgtt tttgataata ttgcatttgg tttaaaagtt aagaaggtag ctccagatgt
     5281 tattaaagct aaagtatcag cagtggcagc aaaaattaag atctctgatc aacagttaca
     5341 gcgtaatgta tcagaattat ctgggggtca acaacaaagg gtagcattgg ctcgtgctct
     5401 ggttcttgaa cctaaaattc tttgtctaga tgaaccattg tcaaaccttg acgcaaaatt
     5461 acgtgtagat ttgagaaaag agttgaaaag acttcaaaaa gagttaggta ttactacttt
     5521 atatgttact catgatcaag aggaagcctt gactttatct gatagaattg cagtctttaa
     5581 caatggatac atcgaacagg tcggtacacc agtagagatt tatcataatt ctcaaactga
     5641 atttgtatgt gattttattg gagatattaa tgttttgacc gatgaaacag tccacgaagt
     5701 attattgaaa aatacaagcg ttttcttaga ggataaaaaa ggatacattc gattagagaa
     5761 agttcgattc aatcgtgaaa ctgaacaaga ttttattcta aaagggacaa ttattgatgt
     5821 tgagttttct ggagttacaa ttcactatac aataaaagtt tctgaaagtc agattcttaa
     5881 tgtaacaagt attgatagtc aggctgctat tagatctgtc ggagaaagtg tggaattatt
     5941 tatcacacca tcagacgttc tgcaatttta aggaggtgca gtatgcgtca taaattaaat
     6001 ttaaaagatt ggcttattcg tttagggtta atctggttct tagtaacatt tattatttat
     6061 ccaaactttg atctagtagt gaatgtattt gtaaaaggag gagaattttc ccttgatgct
     6121 gtacatcgtg ttctaaaatc tcagagggca cttcagagta ttatgaacag ttttaagtta
     6181 gcattttcac tcattattac agttaatgtc gtaggtattc tttgtgttct atttacagag
     6241 tactttgata ttaaaggtgc taaaatttta aaattaggtt atatgacctc tttaatttat
     6301 ggaggagtgg ttttagcgac tggatataaa tttgtctatg gtccttatgg attgattaca
     6361 aaatttttac aaaatgttat cccttcttta gaccctaact ggtttattgg gtatggtgca
     6421 gtcttattca ttatgacatt ttcaggaact gctaatcata cattgttttt aacaaataca
     6481 attcgaagcg ttgactatca cactattgag gctgctcgaa atatgggagc aaaaccattt
     6541 actgttttcc gaaaagtagt gttaccaacc ttaattccaa ctctatttgc acttactatt
     6601 atggtttttc ttagtggttt atctgcagta gcagcaccca tgattgttgg tggtaaagaa
     6661 tttcaaacta taaatccaat gattattaca tttgcaggga tggggaattc tcgtgattta
     6721 gctgccctac ttgcaattat tttaggtatt gcaactacaa ttttgcttac tatcatgaat
     6781 aagatagaaa aaggtggaaa ttatatttct atctctaaga ctaaagcgcc tcttaaaaaa
     6841 caaaaaattg cgtctaagcc ttggaatatc attgctcaca ttgtagcata tggattgttc
     6901 acagttttca tgcttccact aatttttata gtattatact catttacaga tccagttgca
     6961 attcaaacag gtaacttaac attatcaaac tttactttag aaaattatcg cttattcttt
     7021 agtaatagtg cggcattctc tccattcttg gtcagcttta tttattctat tattgctgcg
     7081 acaacagcaa caattctcgc agttgtattt gctcgtgttg tcagaaaaca taaatctcgt
     7141 tttgatttct tatttgaata tggtgctcta cttccttggt tactaccaag tacactttta
     7201 gcagtaagtt tattatttac ttttaatcag ccacaatttc ttgtcttgaa tcagattttg
     7261 gtaggtagtt tggtaattct acttattgca tatatagttg taaaaatccc attttcttat
     7321 agaatggtac gtgctatttt atttagtgtt gatgatgaga tggaagatgc agcaagaagt
     7381 atgggtgctt caccttttta tactatgatg aaggttatca ttccatttat tttaccggtt
     7441 gttctctctg ttattgcttt aaactttaac tctttattaa ctgacttcga cttatctgta
     7501 ttcctttacc atcccctagc tcaaccatta ggtattacga ttcgatctgc aggtgatgaa
     7561 acagcaacat ctaatgcaca agctctggta tttgtttata caattgttct gatgattatt
     7621 tctggaacgg tattatactt cacacaaaga ccggggcgta aagtaaggaa ataatcatga
     7681 cagccactag tcttgggtta tcaaatattg aaatagttgt caggattgtt ttatcagtag
     7741 tcattggtag tataattggt ttagagagag ggagcaaatc ccagcctgca ggcatccgaa
     7801 cttatagtat tgtttgtcta gctgcatgtt tgattatgat gacgaatgaa tacgtatctt
     7861 ataaatttgg gacaggagat cctacacgat taggagctca agttatatca ggtgtgggtt
     7921 ttctaggcgc tggaacgatt cttattacag ataaaaagaa aattacaggt ctgacaactg
     7981 cagcaggcat ttgggcttcg gcaggaattg gattagctat tggagtaggt ttttatgagg
     8041 gagctctttt agtagccatt tctgtttggg gtgtgatatc catgttccaa ccactaaaaa
     8101 aatatctgca aaatcgttct aaaatgattg aattgtatat agtggttaaa tctacagaag
     8161 cctataatcg tgtattggtt tattgtgcag aaaatggtat cagaatgacc gattcaagaa
     8221 cagcgtttgg agatgttaat tctgacagaa ttgaatactt tgatgttccg gacaaaaaaa
     8281 tagcgtcatt tattactctg gaattatcag gtagatttga acatcttcga ctgatggaag
     8341 aaattgctaa tattgtaggt gttatttatg tcgaagaaat tagctgaaat ttagtaaaca
     8401 aagggacaga tgtttttgtc cttttgcttt ttagatgttt taatcatcta atttttgagt
     8461 agatttttgc tcagaggttt ttatgaaaga ttataaaatc aattttgatt taggtaaaat
     8521 agaatatttt gataataatt gtttgataca agtatacaaa tttatttctt tttatgatat
     8581 ttgtgaaatg gtgtttgcct ttcatttacc tcctgatgag ttgataacaa atgttatctt
     8641 taaaga
//
LOCUS       DAVKNL010000075         8281 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVKNL010000075 DAVKNL010000000
VERSION     DAVKNL010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8281
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.075"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(<1..419)
                     /locus_tag="VW411_001847"
     CDS             complement(<1..419)
                     /locus_tag="VW411_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9794526.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFG"
     gene            complement(433..1299)
                     /locus_tag="VW411_001848"
     CDS             complement(433..1299)
                     /locus_tag="VW411_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9794527.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIIAIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFFQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSMVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            complement(1313..2572)
                     /locus_tag="VW411_001849"
     CDS             complement(1313..2572)
                     /locus_tag="VW411_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9794528.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            complement(2664..4826)
                     /locus_tag="VW411_001850"
     CDS             complement(2664..4826)
                     /locus_tag="VW411_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEU9794529.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPGYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLFYHDIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMMRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGLVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTVSNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            4934..5794
                     /locus_tag="VW411_001851"
     CDS             4934..5794
                     /locus_tag="VW411_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU9794530.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFHYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKAPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            5791..6726
                     /locus_tag="VW411_001852"
     CDS             5791..6726
                     /locus_tag="VW411_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEU9794531.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTAIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(6852..6939)
                     /locus_tag="VW411_001853"
     tRNA            complement(6852..6939)
                     /locus_tag="VW411_001853"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6903..6905),aa:Ser,seq:gct)
     gene            complement(6946..7019)
                     /locus_tag="VW411_001854"
     tRNA            complement(6946..7019)
                     /locus_tag="VW411_001854"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6983..6985),aa:Ile,seq:gat)
     gene            complement(7055..7125)
                     /locus_tag="VW411_001855"
     tRNA            complement(7055..7125)
                     /locus_tag="VW411_001855"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7091..7093),aa:Gly,seq:tcc)
     gene            complement(7145..7217)
                     /locus_tag="VW411_001856"
     tRNA            complement(7145..7217)
                     /locus_tag="VW411_001856"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7182..7184),aa:Phe,seq:gaa)
     gene            complement(7221..7294)
                     /locus_tag="VW411_001857"
     tRNA            complement(7221..7294)
                     /locus_tag="VW411_001857"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7258..7260),aa:Met,seq:cat)
     gene            complement(7308..7397)
                     /locus_tag="VW411_001858"
     tRNA            complement(7308..7397)
                     /locus_tag="VW411_001858"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7359..7361),aa:Ser,seq:tga)
     gene            complement(7408..7481)
                     /locus_tag="VW411_001859"
     tRNA            complement(7408..7481)
                     /locus_tag="VW411_001859"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7445..7447),aa:Ile,seq:cat)
     gene            complement(7495..7568)
                     /locus_tag="VW411_001860"
     tRNA            complement(7495..7568)
                     /locus_tag="VW411_001860"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7532..7534),aa:Met,seq:cat)
     gene            complement(7574..7647)
                     /locus_tag="VW411_001861"
     tRNA            complement(7574..7647)
                     /locus_tag="VW411_001861"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7611..7613),aa:Pro,seq:tgg)
     gene            complement(7663..7736)
                     /locus_tag="VW411_001862"
     tRNA            complement(7663..7736)
                     /locus_tag="VW411_001862"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7700..7702),aa:Arg,seq:acg)
     gene            complement(7746..7831)
                     /locus_tag="VW411_001863"
     tRNA            complement(7746..7831)
                     /locus_tag="VW411_001863"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7795..7797),aa:Leu,seq:taa)
     gene            complement(7839..7910)
                     /locus_tag="VW411_001864"
     tRNA            complement(7839..7910)
                     /locus_tag="VW411_001864"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7876..7878),aa:Gly,seq:gcc)
     gene            complement(7933..8005)
                     /locus_tag="VW411_001865"
     tRNA            complement(7933..8005)
                     /locus_tag="VW411_001865"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7970..7972),aa:Thr,seq:tgt)
     gene            complement(8024..8105)
                     /locus_tag="VW411_001866"
     tRNA            complement(8024..8105)
                     /locus_tag="VW411_001866"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8069..8071),aa:Leu,seq:tag)
     gene            complement(8111..8183)
                     /locus_tag="VW411_001867"
     tRNA            complement(8111..8183)
                     /locus_tag="VW411_001867"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8148..8150),aa:Lys,seq:ttt)
     gene            complement(8208..8280)
                     /locus_tag="VW411_001868"
     tRNA            complement(8208..8280)
                     /locus_tag="VW411_001868"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8245..8247),aa:Asp,seq:gtc)
BASE COUNT         2579 a         1857 c         1522 g         2323 t
ORIGIN      
        1 ccaaaggtat tagccaaacc gagtttactc atcataaccg taatcggaat catgatgact
       61 tggaaaggta cgaagattcc gaggattaag agggtataca tgatggtaaa ggcttttctt
      121 ttactcatat tgcgagcgat ggagtaggct gccataggga taaagatcat tactgcaagt
      181 aaagacaaga cagtgatgac gacagagttc caataatagc ctccaatccc atcagctaag
      241 agacggctaa agttgtccca tgtgaagttg gttggaaagc caaagaaatt atctacaata
      301 tccttagtgg gtttgaagga actaaagagg gtagcaagga gcggcactaa aatcagaacc
      361 gatcctagaa tcaatagaat gtatttgcca atcagggctt ttctttcatc ttgtttcatc
      421 atgcttctcc tcttaaattt caaatttctt agatactctc aattggatga tcgaaatcac
      481 tacaattaag aagaacaaga ttacggcaat ggcattggca taaccgaatt ggttgttttt
      541 aaaggcatag ttataaacca agagcccaag tgaggttgtg gcattgtttg gaccaccacc
      601 ggtcatggca aagacttggt caaaggcagt cagcccacct tttagggcta ggataaagac
      661 catagagaca cttggtagca agtaaggcaa ttcaatgttc cagaaaactt gcttgctagt
      721 cgcaccatca atccttgctg cctctgtaat ctcagttgga atagattgca aaccagctag
      781 gaagatgatg atgggcatag ccaccccttg ccaaagaagg acaaagacag ccgcaaagat
      841 tgctccccac ttagtcccta aaagactggt ttggaaaaat tcaatatgaa gggcatttcc
      901 aatcgctgga agaccgtagt tgaagacttg cttgaagatc aaagccactg tcaaaccaga
      961 taaaacagct gggaagaaga accaagcacg gaagaaggtt tggcctttga ttttagaatt
     1021 caagacacgc gcaatgaaga tcccgagtgc aatctcacca accaccatgg caatcgcaat
     1081 gattgcggta aagccaatcg cattcatgaa ttttggatcc atgaagagga gcttaaagtt
     1141 gtttaagcca acaaatttgt agttataagt caatcctgtc cagttggtaa aactgtaaaa
     1201 ggctccttga aacatcggca catagaagaa aattgcttgt aacaagaggg ggatgaccac
     1261 aaaagcccat gcccaatatt tttgtaatac ttttttcata gtctctctac tcctaatcca
     1321 catccgcttt catcgggtta aagaaggcat tcaaatcatt gaccatgcct tgtttatcac
     1381 cggtcaagac atagttcatg gtcaaggtat ggaagtctgc ttcactggtc cagtattgtt
     1441 gcaaccagac caagtgacga tccgtaaagg catattcggt cataccagca agcggtgaat
     1501 cttctcctgc ttgtttgacc ccttcgatcg ctgttggaga tccgtccaca tcgtagtatt
     1561 tttgcatgac ttctggacgg gtcatatatt ccacaaaggc attggcttct tttggatgtt
     1621 tggtggtggc tgagatagac catgccaagt ctcccgcacc aacggttaag ctttgtcctt
     1681 tttcttttcc tggaatcatg aaggtcccaa tcttaaagtt cggtttttgt tcattaatcg
     1741 ctgtgatcgc ccaagaccca tttggtgtca tgaggacatc cccacgtgcg aaggctccga
     1801 taacatcggt atagccagca ccttcccagt tcttttgctt agatccattg atgcgaagga
     1861 tgtccatgac cttgatatca tctttcataa tcggatccga caatttaatg gcatttggtt
     1921 gagaataacg aaggtattga tttgcttctt ttcctccacc tgttgctgtc gcaaaggcta
     1981 attgattgta accattgagt gtccaagcat ctgcacctgc aattccaaat ggtgtttgtc
     2041 ctttagcaac gatatctttg actaactgtt caaattcatc ccaggtttca ggaaccttca
     2101 agcccagttc ttcgaattta tctttgttgt agtaaattcc ataagcatta gctgtaaaag
     2161 gaacgttgta aactttttcg tttacagcat atttttcagc gtagccattt ttcacgcgtt
     2221 tcaggtagtc tttgttgctc aaatcttcaa aaacacctgc ttttgcccat tcttgcagtt
     2281 cgatggactg tgggtaaata ttgaccacat caggcacatc tcctgcgaga acgcgtgtct
     2341 tcaatacttc accagcattt ggtacattga cgactttgac cttgatctta gggttttcct
     2401 tctcaaaatc acgagtgatt tcttccaagg ttttggtcat ttcttttttc tggttgaaat
     2461 actcgatggt cactgtgcca tccgcagatt taccatagtt ggagcaagcg ccgagcccaa
     2521 acaaagctaa acctgtagtt gcaagaagtc cgattttttt ataccattcc attagaaagc
     2581 ctcctttata aatttataca cccttattga actgcacccc aaaagttaga cagaataaat
     2641 ctaacttttg gggtcagtac atatcatagt tttctaaaaa tatactgtct actcaaaaaa
     2701 tctccttggg ataagataac agttaagccc gcatacatta gttctgcacc tgagtaaact
     2761 tcgccatttt cctgtaattt atatagtccc tcttcatcca aatcttttaa ttttaaagtt
     2821 gtttccatgg tctctacaac agataaaacg cgaacgtagg ttacaatcgt ttgatttccg
     2881 taattaaatt gtacagctgc ttcattggat acagtatcag gattaattag tctatactgc
     2941 tgtcctaact gaactactgg tcgtaattct ttatacaagt tcacctgatt agcaatcgta
     3001 gctttctctt catctgataa atttgtcaaa tcaagttcat agcccaaatt tcccatcatt
     3061 gctacaaggc cacgtgtttc taatggtgtc attcgtccca tctgatgatt cggtactgct
     3121 gacacatgag cccccataga aatggttgga tagagatagg atgaaccgta ttgaattggt
     3181 aaacgtgcaa tggcatcagt attatcacta gcccagactt gtgggaaata gcgcatcata
     3241 ccaagatcat ttcgtccacc accaccagag caggactcaa agagaatatg gctgtgcttc
     3301 tctgtcagat aagaaacgag ttcataaagc cccagcatgt actgatgaga ttgcatctgt
     3361 gtctctagat aagttaatcc attccctagc ttagtgatat tgcggttcat atcccattta
     3421 atgtaatcaa tatcatgata aaataggagt tgatctaaga cacttttcaa gtattctact
     3481 acctgaggat tggcaagatt aagtactaat tgattccgag aataagtatg ctcatagcca
     3541 ggaacctgaa tagcccagtc aggatgttga cgatacaaat cactatctac agaaatcatt
     3601 tcgggttcta accaaagtcc aaactgcaaa cctctttcat ggatagctga aatcagactt
     3661 tctagacttc cacccagttt ttcctcatta acaacccaat cacctaaagc acgattatca
     3721 tcaaaacgat tgccaaacca accatcatct aatacaaaaa gttcaatgcc aactttctta
     3781 gcttcatctg ctaactctaa cagtttttct ctctgaaagt caaagtaagt agcttcccag
     3841 ttattgatta gaattggacg ttctttttta gaaaattcac ttagcataat gtgcttcagt
     3901 acaaaattct gactttcatg actaatacca gttaatccct gatctgaatg agtcactaaa
     3961 gctaccggtg tttcaaagta ttcctcagga gctaacttcc aagaaaagtt ttctggatta
     4021 atgccaatag ccacccgaac ttcattcaat tgattttttt gaacaaaagc ttcaaagttg
     4081 ccactataca ttagttgaat agcaaacaca ttcccagcat cctctgtgac tccttgttcg
     4141 catagtagaa gagctggtgt ttgagcatga ccagaagcac ctcggtttga actaatcgaa
     4201 aagattcctt gttctacctg ttgacgtcta acagtctttt cacgagcata agcaccctgc
     4261 agagttacta tttcgtaatc tgcagctgga aaatcagcca taaaagaaaa atctttatgg
     4321 atgacaactt cctgattact attattatct aatttactgt agctagcaat agtcgcatca
     4381 ttattaaaag tagtataata caaagtcaga ctaagttgag ccttagaatc ttctaacatt
     4441 aagacaagag tctctgtatc gtccatgcta tgtggagaag gtaagccctg tggaccattc
     4501 tgacctttta aaatctttgc ttctacaaat cgaaagtctg ttacttcagt tacactatgc
     4561 tgaacctgta tggttggttt cctaaaatct cctaagccat gttgtccaaa aatctgtcgc
     4621 tgagtatcta aactaaaggt tcgattagta gccgttggat ttcctgaaaa ggcatggtct
     4681 cgttcataaa cactattgga acctttatag ttcttaatag tctttcctaa atgtttcaaa
     4741 agtaagtagc catttcgatt ttcaataatc aaacttagat ttttactctc aacataaaat
     4801 agattattct ctatcctaac tcccatttac ttcacctcat cactttattg attatatttt
     4861 atcacctgaa atcgctttcc aaaatagaaa aatgtctcaa gaatatggta aaatgttagg
     4921 taggaggtag cacatgttag ttttttcaga ataccagact ggaacaatcg accttgccct
     4981 aagcttttat ggatatgagg aatgcacacc taattactct tttggtccag ccattcgtga
     5041 tacatacgtt ctacattaca ttactaaagg acaaggaaaa tttcattaca agggtaaaat
     5101 tgttgattta aaagaaggag atttctttct attaaaacca gaggaactaa ccttttatca
     5161 agcagatagt aaagaacctt gggcctacta ctggttagga atcactggag ggaaagcccc
     5221 tgattatttt gctctttccc aaatttctga tcaatcctat ctcatccaat ctgaaacttg
     5281 tcatacccag actactgcaa aactcatctc agacattgtc cgcttcgctc agattacaaa
     5341 atcaagtgaa ttagctcaac tccatatcat gggacaactt catgaactga tgtttcatct
     5401 gggaactatt gctcccaatc agaaaaaaaa gaatatttca tcaacccacc aactctatct
     5461 tgaatgcaaa cgattaattg atagccacta tcctcaatca cttacaattc aagatttagc
     5521 aaaagaacta tccgttcaca gaagctactt atcaagcgta ttcaaagaat ttaatacctt
     5581 atcacccaaa gaatacctac tctacgttcg aatgcaccga gctagacaac ttctcgaaaa
     5641 tacccaagag tccatcaagg taattgcata ctcggtaggt ttttcagatc cactccattt
     5701 ttcgaaagct tataaacaat actttaatca gactccaagt catacaagaa aagaatactc
     5761 tcaataccaa ctagtaagaa aggcaacatt atgaaatcct accaagctgt ctaccaaatc
     5821 ctatctaaag aaaccgacta tatcagcgga gaaaaaatcg cagaaaaact atccctaagc
     5881 cgaacagcaa tttggaaagc catcaagcga ctagaacaag aaggcattga aattgatagt
     5941 atcaaaaata gaggatataa actgatgaat ggtgacctta ttcttccaga gattctagaa
     6001 gaaaatcttc caattaaagt cagctttaaa cccgaaacaa aatcaacaca actagatgca
     6061 aaagaagcaa ttgatttagg ccatgaagca aataccctct atctagcttc ctatcaaaca
     6121 gcaggccgag gccgttttca acgttccttc tactcaccac aaggtggtat ttatatgaca
     6181 ctccatctta aaccaaatct cccctatgac aaattaccat cctacacact acttgtagct
     6241 ggagctgtct acaaagccat taagaaccta actttaatag atgtcgacat aaaatgggtc
     6301 aatgatatct atctaaacaa tcataaaatt ggaggaatcc ttactgaagc aatgacctct
     6361 gtagaaactg gcttagtcac agatatcatt attggagtag gtatcaattt cactattaaa
     6421 gacttccctc aggaattaaa agaaaaagct gccagcttat ttaaagctac agctcctata
     6481 acaaggaatg aattgatcat agaaatctgg cgtgctttct tcgaaacacc agcagaagag
     6541 ctattatacc tatacaaaaa acagtcattc attctaggaa aagaagtcac tttcacacta
     6601 gagcaaaaag actacaaggg acttgctaaa gacatctcag aaaatggaaa acttttagtt
     6661 caatgtgata acggaaaaga aatctggcta aatagtggcg aaatttctct caatagttgg
     6721 aagtaaaata acacaattat aatataaacg atataaaaat aacttcagat tagtaattca
     6781 attaagtttt acggatctga agttttattg gctctaaaaa taaaaaagag agttacagac
     6841 tctcattaaa acggagaata agggattcga acccttgcgc cagttacccg acctaacgat
     6901 ttagcaaacc gtcctcttca gcctcttgag taattctcca attaatgggc acgagtggac
     6961 tcgaaccacc gacctcacgc ttatcaggcg tgcgctctaa ccacctgagc tacgcgccca
     7021 agttaaaaaa cttggtaatt tgaacaaagt tcaaagcggg tgacgagaat cgaactcgcg
     7081 acaacagctt ggaaggctgt agttttacca ctaaactaca cccgcataaa tactatcaat
     7141 aaaatggcgc gagacggaat cgaaccgccg acacatggag cttcaatcca ttgctctacc
     7201 aactgagcta ccgagcctta ttgcgggagc aggatttgaa cctacgacct tcgggttatg
     7261 agcccgacga gctaccgagc tgctccatcc cgcgttaata atataaaagg aggatgtggg
     7321 attcgaaccc acgcacgctt ttacacgcct gacggttttc aagaccgttc ccttcagccg
     7381 gacttgggta atcctccaat attcaaatgg accttgtagg acttgaacct acgaccactc
     7441 ggttatgagc cgagagctct aaccagctga gctaaaggtc cgacaagatc attatagcgg
     7501 cgaaggggat cgaacccccg acctcccggg tatgaaccgg acgctctagc cagctgagct
     7561 acaccgccat gaatcgggaa gacaggattc gaacctgcga caccttggtc ccaaaccaag
     7621 tactctacca agctgagcta cttcccgagt taaatagaaa aatgcaccct agaggagtcg
     7681 aacctctaac cgcctgattc gtagtcaggt actctatcca gttgagctaa gggtgctcca
     7741 tattatgccg aggaccggaa tcgaaccggt acgatcgtta ccaatcgcag gattttaagt
     7801 cctgtgcgtc tgccagttcc gccaccccgg cctctctaag cgaacgacgg gattcgaacc
     7861 cgcgaccccc accttggcaa ggtggtgttc taccactgaa ctacgttcgc actgttttct
     7921 tctatctaaa aatgccggct acatgacttg aacacgcgac cctctgatta caaatcagat
     7981 gctctaccaa ctgagctaag ccggctcatt tgttatatct taatgcgggt taagggactt
     8041 gaacccccac gccgttaagc gccagatcct aaatctggtg cgtctgccaa ttccgccaaa
     8101 cccgcatata tgacccgtac tgggctcgaa ccagtgaccc attgattaaa agtcaattgc
     8161 tctaccaact gagctaacga gtctaaaata acttgcgtta ccttaaacgg tcccgacggg
     8221 aatcgaaccc gcgatcttcg ccgtgacagg gcgacgtgat aaccgctaca ctacgggacc
     8281 t
//
LOCUS       DAVKNL010000076         8248 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVKNL010000076 DAVKNL010000000
VERSION     DAVKNL010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8248
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.076"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            51..188
                     /locus_tag="VW411_001869"
     CDS             51..188
                     /locus_tag="VW411_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794532.1"
                     /translation="MRYQTHYQLKQKLWLEPFGNRQAIGGNELIHSGLFQVYHLGFGS
                     S"
     gene            219..1064
                     /locus_tag="VW411_001870"
     CDS             219..1064
                     /locus_tag="VW411_001870"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEU9794533.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            complement(1211..2272)
                     /gene="dinB"
                     /locus_tag="VW411_001871"
     CDS             complement(1211..2272)
                     /gene="dinB"
                     /locus_tag="VW411_001871"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEU9794534.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKSVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGVSYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILLAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSKKERGVRLLGITMTGF"
     gene            2620..4944
                     /gene="pflB"
                     /locus_tag="VW411_001872"
     CDS             2620..4944
                     /gene="pflB"
                     /locus_tag="VW411_001872"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEU9794535.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            complement(5082..6350)
                     /locus_tag="VW411_001873"
     CDS             complement(5082..6350)
                     /locus_tag="VW411_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU9794536.1"
                     /translation="MEHINHTTLLIGIKDKNITLNKAIQHDTHIEVFATLDYHPPKCK
                     HCKGKQIKYDFQKPSKIPFIEIGGFPSLIHLKKRRFQCKSCRKVTVAETTLVQKNCQI
                     SEMVRQKIAQLLLNREALTHIASKLAISTSTSTSTVYRKLKQFHFQEDYTTLPEILSW
                     DEFSYQKGKLAFIAQDFNTKKIMTILDNRRQTTIRNHFFKYSKEARKKVKVVTVDMSG
                     SYIPLIKKLFPNAKIVLDRFHIVQHMSRALNQTRINIMKQFDDKSLEYRALKYYWKFI
                     LKDSRKLSLKPFYARTFRETLTPRECLKKIFTLVPELKDYYDLYQLLLFHLQEKNTDQ
                     FWGLIQDTLPHLNRTFKTTLSTFICYKNYITNAIELPYSNAKLEATNKLIKDIKRNAF
                     GFRNFENFKKRIFIALNIKKERTKFVLSQA"
     gene            complement(6615..8144)
                     /gene="rlrA"
                     /locus_tag="VW411_001874"
     CDS             complement(6615..8144)
                     /gene="rlrA"
                     /locus_tag="VW411_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus transcriptional regulator RlrA"
                     /protein_id="HEU9794537.1"
                     /translation="MLNKYIEKRITDKITILNILLDIRSIELDELSTLTSLQSKSLLS
                     ILQELQETFEEELTFNLDTQQVQLIEHHSHQTNYYFHQLYNQSTILKILRFFLLQGNQ
                     SFNEFTQKEYISIATGYRVRQKCGLLLRSVGLDLVKNQVVGPEYRIRFLIALLQFHFG
                     IEIYDLNDGSMDWVTHMIVQSNSQLSHELLEITPDEYVHFSILVALTWKRREFPLEFP
                     ESKEFEKLKNLFMYPILMEHCQTYLEPHANMTFTQEELDYIFLVYCSANSSFSKDKWN
                     QEKKTHTIQLILQHTRGKHLLSKFKNILGNDISNSLSFLTALTFLTRTFLFGLQNLVP
                     YYNYYEHYGIESDKPLYHISKAIVQEWMTEQKIEGVIDQHRLYLFSLYLTETIFSSLP
                     AIPIFIILNNQADVNLIKSIILRNFTDKVASVTGYNILISPPPSEEHLTEPLIIITTK
                     EYLPYVKKQYPKGKHHFLTIALDLHVSQQRLIYQTIVDIRKEAFDKRVAMIAKKAHYL
                     L"
BASE COUNT         2453 a         1525 c         1667 g         2603 t
ORIGIN      
        1 ggttatccac gaattttttg ccttgttacc ttagacgaga tcaaacgtct atgcgttatc
       61 aaactcatta ccaattgaaa caaaagctgt ggttagagcc tttcggaaat cgtcaagcga
      121 ttggaggaaa tgaactaatc catagtggct tattccaagt ataccacttg ggctttggca
      181 gtagctaact gcgctaaata taatataggg agtaatctat gtatcttatt gaaattttaa
      241 aatctatctt cttcggaatt gttgaaggaa ttacggaatg gttgccgatt tccagtacag
      301 gtcacttgat tttagcagag gaattcatcc aataccaaaa tcaaaatgaa gcctttatgt
      361 ccatgtttaa tgtcgtgatt cagcttggtg ctattttagc agttatggtg atttatttta
      421 acaagctcaa tccttttaaa ccgaccaagg acaaacagga agttcgtaag acttggagac
      481 tatggttgaa ggtcttgatt gctactttac ctttacttgg tgtctttaaa tttgatgatt
      541 ggtttgatac ccacttccat aacatggttt cagttgctct catgttgatt atctacgggg
      601 ttgccttcat ctatttggaa aagcgcaata aagcgcgtgc tatcgagcca agtgtaacag
      661 agttggacaa gcttccttat acgaccgctt tctatatcgg actcttccaa gttcttgctc
      721 ttttaccagg gactagccgt tcaggtgcaa cgattgtcgg tggtttgtta aatggaacca
      781 gtcgttcagt tgtgacagaa tttaccttct atcttgggat tcctgttatg tttggagcta
      841 gtgccttaaa gattttcaaa tttgtgaaag ccggagaact cttgagcttt gggcaattgt
      901 ttttgctctt ggtcgcgatg ggagtagctt ttgcggtcag catggtggct attcgcttct
      961 tgaccagcta tgtgaaaaaa cacgacttca ccctttttgg taaataccgt atcgtgcttg
     1021 gtagtgtttt gctactttac agttttgtcc gtttatttgt ataagaaaaa ccttgaaggg
     1081 gcaactcttc aaggttttat actcttcgaa aatctcttca aaccgcgtca gctttatctg
     1141 caacctcaaa acagtgtttt gagcagcctg cggctagcct cctagtttgc tctttgattt
     1201 tcattgagct ttaaaatcca gtcatggtaa tccccaatag gcggacacct ctttctttct
     1261 tgcttaattc ttcatagagt tgcagggcta tttggcttat ctgactagca tcttgtgttt
     1321 tttgagcaag actttttcgt ttggtaagag ttgaaaagtc ctcgtagcgg attttcaaaa
     1381 tgacaatttt tccagctttt tcttgttgat gtagattgag agcgactttt tctgatagaa
     1441 gagtcagctc ttttttgata tcttcctcag caaggagaat cttcccgtag gttttctcct
     1501 tgccgattga tttacggatg cgattggatt tgactggaga gttgtgaatg ccacgagcct
     1561 ttcgatacag atcatagcct agtctaccaa aacggtctat tagggttacc tcaggaactt
     1621 caagtaaatc agcaccagta aaaacgccca tttgatgaag acgttctact gtcttttttc
     1681 ctactccatg aaatttggaa atatccattt gtttgagaaa atcctcagcc tgttcaggta
     1741 gaatcactgt caaaccatgt ggtttttgat aatcactcgc cattttagct aagaatttgt
     1801 tgtaagaaac gcctgcggaa gcagttagat ggagttcttg ccagatatct ttttgaatga
     1861 ggcgagcaat tttgaccgct gacttgatac cgagtttatt ttctgtcaca tccaaatagg
     1921 cttcgtcaat gctcatgggt tcaatcaaat ctgtatagcg cttaaaaata gctcgaatct
     1981 ggagtcccac agacttgtat ttctcataat tccctgagat aaagacagcc tggggacaac
     2041 gttcataagc ttccttggaa ctcatggcag aatggacacc aaaagctctt gcctcataac
     2101 tacaggtaga aacgactccc cgtccacctg tttgccgagg gtcgcttcca ataatgacag
     2161 gttttcctct gagtttagga ttatccctga tttccactgc agcaaaaaag gcatccatgt
     2221 caatatggat gatttttctt gacaaatcat ttaacaaagg aaaaatcaac atgcctagca
     2281 cctttttata ctcttcgaaa atctcttcaa accacgtcag ctctatctgc aacctcaaaa
     2341 cagtgttttg agcaatctgc ggctagcttc ctagtttgct tttcgatttc cattgagtgt
     2401 tactgcttat tttcttttat tatacccttt tttctgaaaa aaagaaaaaa ggactttatt
     2461 ttttcaaaaa tataatacag tttgaaataa aatatagact gttttagaaa agaaagtgta
     2521 aaaataggga attttcactt gttgaaatcg gttactatat ggtatacttg tcttatgaat
     2581 gtaacagatg actgttacta gaaaaaagag gacattaata tggttgttaa gacagttgtt
     2641 gaagcacaag atatttttga caaagcttgg gaaggcttca aaggcgtaga ttggaaagaa
     2701 aaagcaagtg tatcacgatt tgtacaagct aactacacac cttatgatgg agacgaaagc
     2761 ttccttgcag gaccaacaga gcgttcactt cacatcaaga aaattgtaga agaaactaaa
     2821 gcacactacg aagaaactcg tttcccaatg gacactcgtc caacatctat cgctgatatc
     2881 cctgctggat ttatcgacaa agaaaatgaa gttatcttcg gtatccaaaa cgatgaactc
     2941 ttcaaattga acttcatgcc aaaaggtggt atccgtatgg ctgaaactac tttgaaagaa
     3001 aatggatacg aaccagaccc agctgttcac gaaatcttca ctaaatatgt aacaacagtt
     3061 aacgacggta ttttccgtgc ctacacttca aatattcgtc gcgctcgtca cgcacacact
     3121 gtaactggtc ttccagatgc atactcacgc ggacgtatca tcggtgttta cgcacgtctt
     3181 gctctttacg gtgcagacta cttgatgcaa gaaaaagtaa atgactggaa tgcaatcaaa
     3241 gaaatcgatg aagaaacaat ccgtcttcgt gaagaagtaa accttcaata ccaagcattg
     3301 caacaagttg ttcgcctggg tgacctttac ggggttgatg ttcgcaaacc agcgatgaac
     3361 gtgaaagaag caatccaatg ggttaacatt gctttcatgg ctgtctgccg tgtgattaac
     3421 ggtgctgcta catctctagg tcgtgtacca atcgtattgg acatctttgc agaacgtgac
     3481 cttgctcgtg gtacatttac tgaatcagaa atccaagaat tcgttgatga tttcgttatg
     3541 aaacttcgta cagttaaatt tgctcgtaca aaagcttatg accaattgta ctcaggtgac
     3601 ccaaccttta tcacaacttc tatggctggt atgggtaacg acggtcgtca ccgtgttact
     3661 aagatggact accgtttctt gaacactctt gacaacatcg gtaactcacc agaaccaaac
     3721 ttgacagttc tttggactga caaattgcca tacaacttcc gtcgctactg tatgcacatg
     3781 agccacaaac actcttctat ccaatacgaa ggtgtaacaa caatggctaa agacggatat
     3841 ggtgaaatga gctgtatctc atgctgtgtg tctccacttg atccagaaaa tgaagaacaa
     3901 cgccacaaca tccagtactt cggtgctcgt gtaaacgttc ttaaagccct tcttactggt
     3961 ttgaatggtg gttacgacga tgttcacaaa gactacaaag tatttgatat cgaaccaatc
     4021 cgtgacgaag ttcttgaatt tgaatcagtt aaagcgaact ttgaaaaatc tcttgactgg
     4081 ttgactgaca cttacgtaga tgccttgaac atcatccact acatgactga taggtacaac
     4141 tacgaagctg ttcaaatggc cttcttgcca actaaacaac gtgccaacat gggattcggt
     4201 atctgtggat ttgctaacac tgttgataca ttgtcagcta tcaaatacgc tacagttaaa
     4261 ccaatccgtg acgaagatgg ctacatctac gattacgaaa caatcggtga ctacccacgc
     4321 tggggtgaag atgacccacg ttcaaacgaa ttggcagaat ggttgatcga agcttacaca
     4381 actcgtctac gtagccacaa actatacaaa gacgcagaag ctacagtatc acttttgaca
     4441 atcacatcta acgttgctta ctctaaacaa actggtaact caccagttca caaaggtgta
     4501 tacctcaacg aagatggttc tgtgaacttg tctaaacttg aattcttctc accaggtgct
     4561 aacccatcta acaaagctaa aggtggttgg ttgcaaaact tgaactcact ttctagcctt
     4621 gactttagtt atgcagctga cggtatctca ttgactacac aagtatcacc tcgcgctctt
     4681 ggtaagactc gtgatgaaca agttgataac ttggtaacaa ttcttgatgg ttacttcgaa
     4741 aacggtggac aacacgttaa cttgaacgtt atggacttga acgatgttta cgaaaaaatc
     4801 atgtcaggcg aagacgttat cgtacgtatc tctggatact gtgtaaacac taaatacctc
     4861 actccagaac aaaaaactga attgacacaa cgtgtcttcc acgaagttct ttcaatggat
     4921 gacgccttgg atgcattgag ctaatcaagt tcttgaataa taaaaaggaa ccctcggtca
     4981 aacgactgag ggttttgtgc ttgggatagt atgagcaatt ccttcggcgc aatatgcaat
     5041 gtttttgggc tctttgtcaa ctgtagtggg ttgaaaaaaa gctaagcttg agaaaggaca
     5101 aatttcgtcc tttctttttt gatgttcagg gcgataaaaa tccgtttttt gaagttttca
     5161 aagttccgaa aaccaaaggc attgcgcttg atgtctttga tgagtttgtt agtggcctca
     5221 agtttagcgt tagaataagg caattcaatg gcgttagtga tgtagttttt atagcaaata
     5281 aatgtgctca aagtggtttt aaaggtgcgg ttgagatgag gtaacgtgtc ttgaattaag
     5341 ccccaaaact ggtcagtatt cttctcttgt agatgaaata ggagtagttg atacaggtca
     5401 tagtaatctt taagttcagg tactagagta aagattttct tcagacactc cctaggagtt
     5461 aaggtctctc tgaaagttct agcatagaaa ggcttaagag agagtttccg actatctttt
     5521 aggataaatt tccagtaata tttaagagct ctgtattcca gagatttatc atcaaattgc
     5581 ttcatgatgt tgattctagt ctgattaaga gccctgctca tgtgttggac aatgtggaaa
     5641 cgatcgagaa caattttagc attgggaaat aatttcttaa tgagagggat ataacttcca
     5701 gacatatcaa cagtgacgac tttaactttt tttctagctt ctttcgagta cttgaagaaa
     5761 tgatttcgga tggttgtttg acgtctgtta tcaagaatgg tcatgatttt cttagtgttg
     5821 aaatcctgag caatgaaagc caatttcccc ttctggtagg agaattcatc ccaggagagg
     5881 atttcaggca aagtggtgta atcctcttgg aaatgaaatt gcttgagctt acgatagacg
     5941 gtagaggtag aggtagaggt agagatggct aatttagaag cgatatgtgt aagagcctct
     6001 ctgttgagta ggagttgggc aattttctgt ctcaccattt ccgagatttg gcaatttttc
     6061 tgaacgagag ttgtttcagc tacagtgact ttccgacagg acttgcattg aaatcgtctc
     6121 tttttcaaat gaatgaggct agggaaacca ccaatctcga taaaagggat tttagaaggc
     6181 ttttggaagt cgtatttgat ttgttttcct ttacagtgtt tacatttagg tgggtgataa
     6241 tcaagtgtag cgaagacttc gatatgggta tcgtgctgaa tggctttatt taaggtgatg
     6301 tttttgtctt ttattccgat gagtaatgtg gtatgattga tgtgttccat aagatacttt
     6361 ctaatgagtt gtttaggcgc ttttcattat aagtcttatg ggactttttt gatactcaaa
     6421 aagccctata atctccacag tgggatttac ccactacaga aattatagag ccagaaaaaa
     6481 cacttttgtt cactagcaga aactagagag cagaagtgtt tttctgttca gatttaccca
     6541 aaactgggaa atatggggat aagaatagag atggcttagg aagccccttt ttgtgtgtag
     6601 acagtacgat gaacttataa caaatagtga gcctttttag caatcattgc gacccgtttg
     6661 tcaaaagcct cttttcggat atctacaatt gtctgataga tgagacgctg ttggctaaca
     6721 tgcaaatcta aggcaatcgt caaaaagtga tgtttccctt tgggatactg ctttttaacg
     6781 taaggcaggt attctttcgt tgtaataata atcaatggct ctgtcaaatg ctcctctgaa
     6841 ggaggaggac taattagaat attgtatcct gtaacagagg caactttgtc agtaaaattc
     6901 cgtaaaataa tggactttat taagtttaca tctgcttgat tatttaaaat gataaaaatc
     6961 gggatagcag gtagtgagga aaagatggtt tctgtcaagt agagtgagaa aaggtacagc
     7021 cgatgctggt cgataactcc ttcaatcttc tgctcagtca tccactcttg aacaattgct
     7081 ttcgaaatat gatacagtgg cttgtcgctt tcaatcccat aatgttcgta ataattataa
     7141 tagggaacta gattttgtaa accaaacaaa aacgttcttg ttaagaaagt cagtgctgtt
     7201 aaaaaagaaa gagaattcga aatgtcattt cctaagatat tcttgaactt ggatagtaga
     7261 tgctttcctc ttgtatgctg aagaatcagt tgaatagtat gagtcttttt ttcttgattc
     7321 catttgtcct tggaaaacga agaattagca gaacaataaa ccaaaaagat ataatccagt
     7381 tcttcctgag taaaagtcat gttggcatgt ggctctaagt aagtttggca atgttccatc
     7441 aaaatcggat acataaagag gttttttaat ttttcaaact ctttggactc agggaactca
     7501 agtggaaatt cccgacgttt ccaagtgagt gccactagta tgctaaaatg aacatactcg
     7561 tcaggtgtga tttctaacag ttcatgactg agttgagaat tagactgcac aatcatatgt
     7621 gtgacccaat ccatacttcc atcattcaaa tcataaatct caataccaaa atgaaactgg
     7681 aggagtgcaa ttaaaaaacg aatgcgatat tcaggaccaa ctacttgatt tttcacaagg
     7741 tccaaaccta ctgaacgtag taacaagcca cacttttgtc gtacgcggta gcctgttgcg
     7801 atggaaatat actctttttg tgtaaattcg ttaaagcttt gattaccttg tagtagaaag
     7861 aagcggagta tttttaaaat agttgattgg ttataaagct gatggaagta ataattcgtt
     7921 tgatgagaat ggtgttcgat taattgaact tgttgcgtat ctaaattaaa tgtcaactct
     7981 tcctcgaatg tttcttgtaa ttcctgcaaa atgcttagga gacttttaga ttgtaatgaa
     8041 gttaaagtag acagttcatc tagttcaata gaccgaatat ccaataatat atttaaaatg
     8101 gtaattttat ctgtaattct tttttcaatg tatttgttta gcatagttac cgaatcttag
     8161 ttgcatatag ataattttaa ttattataat acaaaagaaa ctaattgtct tgtcaaaaag
     8221 gttgtggaat ttccgacttt attgataa
//
LOCUS       DAVKNL010000077         8114 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVKNL010000077 DAVKNL010000000
VERSION     DAVKNL010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8114
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.077"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            63..710
                     /locus_tag="VW411_001875"
     CDS             63..710
                     /locus_tag="VW411_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEU9794538.1"
                     /translation="MVVGIAIASGYLNSHLDKFVDWGSWTALVPFGLISVTNVGISML
                     STRFTGKLSKWGNYFGIVNTILSGAIDYILGNKAAIITYPVTFLIYTFAIKKWEASQE
                     GRPNQMSQKQVKLAAIIISIIAFLFAFVTNYIGYGGKMNLLAYVTTIAFALSLIANAL
                     NALKLTTQWGFWLIYNFVQLTKAGIQGNFANIGKYIFYIFNAIGALFVWNDEEVR"
     gene            841..1254
                     /locus_tag="VW411_001876"
     CDS             841..1254
                     /locus_tag="VW411_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794539.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFEWLLLSGLIFFLA
                     QGLLLFLLVVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLRENYPDFYDKHISLWECL"
     gene            1273..1458
                     /locus_tag="VW411_001877"
     CDS             1273..1458
                     /locus_tag="VW411_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794540.1"
                     /translation="MKKIIFIKTIQLLVIDGIMLAFLTFKRGLTWDWILIYSGWLIFF
                     HPVLLTYLSNQLCDHFS"
     gene            complement(1725..2168)
                     /locus_tag="VW411_001878"
     CDS             complement(1725..2168)
                     /locus_tag="VW411_001878"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEU9794541.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKRGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(2198..4420)
                     /locus_tag="VW411_001879"
     CDS             complement(2198..4420)
                     /locus_tag="VW411_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEU9794542.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRMIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYPSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            5391..6020
                     /locus_tag="VW411_001880"
     CDS             5391..6020
                     /locus_tag="VW411_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU9794543.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(6143..8035)
                     /gene="pepO"
                     /locus_tag="VW411_001881"
     CDS             complement(6143..8035)
                     /gene="pepO"
                     /locus_tag="VW411_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEU9794544.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
BASE COUNT         2200 a         1880 c         1559 g         2475 t
ORIGIN      
        1 ctatgaaaaa atcactaaaa atttttgcta catctaaata gtttgacctc ttcggggttg
       61 ctttggtcgt tgggattgcg attgcatctg gttacctcaa ctcacacctc gataaatttg
      121 tagactgggg atcatggaca gctcttgtac cctttggatt gatttccgta accaacgttg
      181 ggatttccat gttgtccact cgtttcacgg ggaaattaag caaatgggga aattactttg
      241 gtattgttaa taccattttg tccggtgcta ttgattatat ccttggaaat aaggcggcca
      301 tcattaccta tcccgtcacc ttcctcattt atacctttgc tataaagaag tgggaagctt
      361 cgcaagaagg cagacccaac caaatgagcc aaaaacaggt aaaattggcg gccatcatca
      421 tttccatcat cgccttcctc tttgcctttg tgaccaacta tatcggatat gggggcaaga
      481 tgaatctcct tgcctacgta acaactattg cctttgcact gtccctcatt gccaatgctt
      541 tgaatgcatt aaaactaaca actcagtggg gcttttggtt gatttacaat ttcgttcagc
      601 tgacaaaagc tggtattcaa ggaaacttcg ccaatatcgg taaatacatc ttttatatct
      661 tcaatgcaat cggagcttta tttgtctgga atgatgaaga agttagataa tagaaggaaa
      721 ctcaaataaa gaatactagc caacttaaga gaatctccct tctgctattt ttatcatttt
      781 agttgtaact cccattcttt tggagtagaa tgaagttaga taaaagaggg aggtcttgtc
      841 atgaaaaaac tctatagaat ccattttata gcaattgcag ttattgattt gctactattt
      901 gcatttttta tcactagact cgagacatct tttgagtggc tcttgctctc tggtttaatt
      961 ttcttccttg ctcaaggact cctgctattt ctcttggtcg tccgactcaa acatcaattc
     1021 gctgagattt atcctcaaat caataaaaag attcgcttct actatttagg ggttctcacc
     1081 attgattttc tattttttgt tctcttagcc ttcattagtt ctcagcgttt ttcatctctt
     1141 atgccaatca tcactgcttg ccattctact ttttattata tgacagctga ctacctaaga
     1201 gaaaactatc cagactttta cgacaaacac atctctttat gggagtgtct ctaaagaaaa
     1261 ggaggtttta gcatgaaaaa aatcatcttc atcaaaacca ttcaactcct tgtcattgat
     1321 ggaatcatgc tggcattttt gacatttaaa agggggctta cttgggactg gattttgatt
     1381 tatagcggtt ggctcatttt ctttcatcct gtgctattga cctatctttc aaaccaactt
     1441 tgtgaccact ttagttaact ctattcccag attagaccga gattctggcg ttttgcttta
     1501 caaattctcc tatgggatag cctgatgatt ctctccttgg tgtctttaag tgatattcca
     1561 cttttccttc agggaactct cctcatccta ggacatctca tcccttccta tcgcatctgc
     1621 caaagcctga aaagagactt cccccaagca tatcaagaac cgatttcttt ttggagtatt
     1681 ttatgataga tgagaaagac caagccgact gggcttggtc tttcttatct ctttttagta
     1741 tctaggataa tggtaacagg tccattatta accagctcaa cctgcatatc tgctccaaag
     1801 atgcctgtct gaacgggcac ttcttgcgct aatttttgat tgaaagcatc atagaagtct
     1861 gatgccatat caggtttagc tgcccctgta aaggctggac gattgcctct cttagtatcc
     1921 gcaaagaggg taaactgaga aatagagagg atttctcctt caatatcttt gacagacagg
     1981 ttcatcttgc cttctgcgtc tgaaaaaatc cgcatattga ccagttttct cacagcatag
     2041 tccaaatctt cctcttggtc ctctggtcca acaccaacca gcaataaaag tccctgattg
     2101 atttttccct gaatctggcc ttctatactc acttgggctt ttttaacccg ttggataatg
     2161 attttcataa tagcctttct agtaagagct aggacaacta gccgttggtc cgtttgacag
     2221 agtaaacttc tggcacactc ttaattttat cgacaaccgt ggtcagtgta gagaggttgg
     2281 caataccgaa ggacacatgg atattagcaa acttcatatc cttggttggt tgggcattga
     2341 ccgttgaaat attcttggtt gtatttgaaa gaacttgcag tacatcgttc aacagtcctg
     2401 tacggttgag accgtagata tcgatatggg ccatatactc cttatttgag ctagggtact
     2461 ggtcttccca ttccacatca aggagacgtt gctcgtagtt ttcttgggca cgcaggttca
     2521 tacagtccac acggtgaata gccacaccac gacccttggt aatgtagcca acaatatcgt
     2581 caccaggcac ggggttacaa cacttagcaa tccgcactag gagaccagaa gcaccttcaa
     2641 taaccactcc cccctcatgc ttgaccttga gggtttcttt attttcaacc ttgacctcgc
     2701 cacctttgac aagctcctct gcctcagctt tggccttggc acgctcttcc tcacggcgtt
     2761 ccttttcagt cagacggtta aagacggtaa tcgcaccgat ttccccaaaa ccaatggccg
     2821 caaagaggga gtcttctgtc ttgtaactgg tcttttgcag aacttgatcc atgtggcgct
     2881 tgtccataaa tttatttgcc acatagccat tttcttggaa ctgagccatc agcatctcac
     2941 gacccttgtt gacagacaat tccttatctt ggtttttaaa gaactggcga atcttattgc
     3001 gcgccttgct agtcttgacc atattgagcc agtcacggct aggtccaaag gagttcgggt
     3061 tggcgataat ttcaacctga tcccctgtct ttaacttggt tgtcagtgga accatgcggc
     3121 cattgacctt ggcaccagtt gctttttcac cgaccttggt atggatttcg taggcaaaat
     3181 caatcggtcc tgaatctttg ggaagggaac ggacagctcc atctggggta aaaacgtaaa
     3241 tctcctcagc caaatagttt tccttaacag agtccacaaa ttccttagca tcatcagcct
     3301 ggtcttggag ctccatcatc tccttgatcc agttcattcc aatagctgat tccttgctgt
     3361 taacttgccc ctttatacct ttcttataag cccagtgagc cgcaaccccg tactcagcca
     3421 cctcgtgcat ttccttggtt cgaatctgga attcaatcgg cccttttggt ccataaacag
     3481 tcgtatggat agactgataa ccattggcct tgcggttggc gatatagtct ttgaagcgac
     3541 ctggcatcgg tttccaaaat tcatgcacgt aaccaagcat ggcataaaca tcactttggg
     3601 tatctaaaat acaacgaata gcaatcagat catagatttc ctcaaaccgt tttctcttgt
     3661 cctgcatttt gcggaaaatt gagtaaatat gcttgggacg accataaatc ttccctttca
     3721 agtgacgttc tgtcgtatac tcctctaatt ttgtgactac ctcatccacc aaggcctcac
     3781 gctccctgcg cttttccttc atcatatggg taatcttgta aaactccgtt ggattgagat
     3841 aacggaaaga caagtcttct aattcccatt tgacactgga aatccccaaa cgatgggcaa
     3901 gcggggcata gatttccatg gtttctttgg aaatacgctc ctgcttgtct tttcgaagat
     3961 gtttcagggt ccgcatattg tgcaagcggt cagacagttt gaccaaaata acgcggatgt
     4021 cctcagacat ggccatgagc atcttgcgat gattttccgc taattgctcc tcgatcgatt
     4081 tgtactcgac cttgccaagc ttggtaactc cgtcaacaat catccgcaca tcaggaccaa
     4141 actctctttc caaatcgtcc aaagtcgcat ctgtatcttc caccacatca tgcaagaatc
     4201 cacaagctac tgttacagca tccagcttta gcttagctaa aatacctgcc acttggatag
     4261 ggtgaatgat ataaggctcg cctgatttgc gatattgacc actgtggcat tcaacagcat
     4321 agaccaaggc cttatggaca aaatgaacat cctcttccgt taaatattct ttggttaaag
     4381 cgacaacttc ttcgcctgtt aaattcactt ctttcggcat ctctactctc caattcttcc
     4441 taccatttta tcactttttt aagaatatga aaactagatt ggaacagaat aagaaaaaaa
     4501 taattcaaaa ttgcttgata attctgaatt attggtccgt aatatactac gaagttagat
     4561 tttaaactta ggtgatagaa ggagagatag aaggagagat agaagaacgg aaaccatatt
     4621 gtaacccaaa gactttctga cttccccaat tccattgaag atacgaaaga taaacggtgg
     4681 aactcgtatc acatacactg gtaccttgac tggattttgg aattaatact aaatgaaaat
     4741 caaagagcaa actaggaaac tagccgcagg ttactcaaag caccgctttg aggttgcaga
     4801 taaagttgac gcggtttgaa gagatttttg aagagtataa aaatcctcaa gatactttct
     4861 tctatccttt agtttataag gagaatacct atgaaaaaaa ctgctatttc tatctttgct
     4921 ctcctaatgt taggagtttg ctgcctgttc ctattcagcc agcaaagcta taaaaaacag
     4981 tcgttcaata ctatgctaac gaccagaacc tgcccagtag gataacttat agtgaatata
     5041 gcgacaaatg agaagccaac tacggtagca ctctaaacat cacgtctatc aaacaagcta
     5101 atgacggagt ttatgcaacc tatgaagggc aattgacacc tttccaatat tgataaattg
     5161 ataaccagcc tgtcttcatc tagtcatgct ggtttttaag ttcattttaa atccttacct
     5221 attctcccta actgtgctat acttaattta tactcaatga aaatcaaaga gcaaactaga
     5281 aagctagccg caggctgttc aaagcactgc tttgaggttg cagataaagt tgacgcggtt
     5341 tgaagagatt ttcgaagagt attagtacat tctttgagat tggagctagt atgaaaatcc
     5401 ataaaaccgt gaatcctgtt gcctatgaaa atacctatta tctagaaggc gaaaagcacc
     5461 tcatcgtcgt cgatcctggt agtcattggg aagccattcg tcagacaatc gagaagatca
     5521 acaaaccgat ctgtgctatt ctcttgaccc acgcccatta tgaccatatc atgagtctgg
     5581 acttggttcg cgagacgttt ggcaatcctc ctgtctatat cgcagagagc gaagccagct
     5641 ggctctacac tcctgtcgat aatctctccg gtctccctcg ccacgatgat atggcagatg
     5701 tggtcacaaa acctgcagaa cacacctttg tctttcacga agaataccaa ctagaggaat
     5761 ttcgttttaa ggttctaccg accccagggc actctatcgg tggtgtttcc ctagtctttc
     5821 ctgatgctca tctagtcttg acgggagatg ctctattccg cgaaactatc ggacggaccg
     5881 accttccgac tggtagcatg gagcaactcc ttcatagtat ccagacccaa ctcttcaccc
     5941 taccaaacta cgatgtctat ccaggacatg gtccagctac tactatcgct cacgaaaagg
     6001 ccttcaatcc ctttttctag caagatgatg acaatcgaaa tttaagtaaa ctatccagca
     6061 aatctttcta ttacaaaagg catcctatca aggttttcac acatgattgg atgccttttt
     6121 tctgatgact agattttttg cattaccaaa taatcacgcg ctcctctggt gaacgccaca
     6181 ttccgtctcc ttctttgaca tcataggttg taaagaaatc gtcgaagttt ggtacttgca
     6241 cattgacacg gagtttggct ggtgcgtgca catcgacgct agccaaaagt ttcataaatt
     6301 ctggtcgacc tttcatgcgc cagatgcgac cgaagttgta gaagaactct tctgctgaga
     6361 agtctgcttc tctcttagct gcttcaagcg ctgctgcgat tcctcccaag tcagccacgt
     6421 tttctgatac agtcaattta ccgttaatgg ttgctccata agaatcctgt ccatcaaatt
     6481 ggtcaatgac tttttgtgtt ttctccttga aggcagcata gtcgctctct gtccaccaat
     6541 ccttgaggct accattttcg tcaaaggaag ccccgttagt atcaaaggcg tgggaaattt
     6601 catgggcaat cactgcccca ataccaccgt agttagcaga agatgactga tgcaagtcat
     6661 agaaaggcgc ctgtaaaatg gccgctggaa agacaatcag gttcttctga ggattgtagt
     6721 aggcattgac catatgagca ggcatgcccc attccttata atctacaggc tggttccact
     6781 tactccaact gtgcttgatt tccacacgcg caaaggctag agcattctca aaaagactgg
     6841 cagtttcatt cactacctta tccttgtaac gtgcaggcaa ttcttctgga tagccaatat
     6901 aaggtttgat cacattgagc ttcacgatag cctgtttaca ggtttctgga gtgagccagt
     6961 cattcttaag cagacgctcc ttataaacat caatcatggt tgccactttt ttctccacat
     7021 ccgccttggc ttctggagag aacttctcac gggcgtacca aagacccagg gcttgcttga
     7081 aaggttcttg tgctagatga taagctgctt tgaccttatc ttttgcctct ggaactccag
     7141 aaagggcacg gctgtaggca ccagacaaaa cacggatatc ctctgttaaa tagctggttg
     7201 aaagattgac aacactcaaa atcaaggttg ctttaaggag agaccaggct tcctcactgt
     7261 agaattgctc tgctgcttgc cagaaacgtt cctcgtctac aataaccttg tctggtaatt
     7321 gcccaataac tgctttgaag aagtcatcca aaggtagggc aggcgcgaat ttcttgaaat
     7381 cttcgtaaga atatggatga tagagtttag catattctga actttcttca ttagagagca
     7441 ccactgccgc aactcggcgg tccaattcaa gtcttttttc tagcaagtct tcaatttctt
     7501 catcagagaa atcataagcc ttgaggagat ttgcgctgct ttctttccaa agagtcaaga
     7561 gctcttcgcg ctgaggatgt tcttctgcat agtaggtcgt atctggcaag attgtgcttg
     7621 gagcgctagc ccatagaaca ttgattctag catccataaa gtctggcgat acaccaaaag
     7681 gaaggaagtt tggttttcct gcaagctcaa actctgctag tttagctgta aaatccgcaa
     7741 aagtctccaa ttcttggaat tctttaagga gtggtaagac aggtgtgata ccgtcagctt
     7801 ctctcttgtc aaaatcacga actaggcggt ggtatttgac aaagttttcc aagatagcat
     7861 cctcaggcac ttcttcacct gctaaccact tgtctgttgt cgccagcatc aggtcttcaa
     7921 tttcctggtc taaatcaaca aaacctcctg tttgagactt atctgctggg atttcagctg
     7981 tctgttgcca ttctccattg atagcatcat aaaaatcatc ttgataacgt gtcatcttgt
     8041 tctcgctttc atttgtattt gcatttatct taacaaaaat cactagggaa tgcaattaat
     8101 aaccatttaa ctat
//
LOCUS       DAVKNL010000078         7889 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVKNL010000078 DAVKNL010000000
VERSION     DAVKNL010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7889
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.078"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            <1..341
                     /locus_tag="VW411_001882"
     CDS             <1..341
                     /locus_tag="VW411_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9794545.1"
                     /translation="ITVNYCHDMKLFKMSCRNIGQAGKILADSGYQGLMKICPQAQTP
                     RKSSKLKPLTAEDKAYNHALSKERSKVENIFAKVETFKMFSTTYRNHRKRFGLRMNLI
                     AGIINHELGF"
     gene            complement(1079..1339)
                     /locus_tag="VW411_001883"
     CDS             complement(1079..1339)
                     /locus_tag="VW411_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794546.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADMHRFVIELEPVKSTILQNNDISLDQNEIFEILKNFLDG"
     gene            complement(1398..1949)
                     /locus_tag="VW411_001884"
     CDS             complement(1398..1949)
                     /locus_tag="VW411_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794547.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFPSMIELFSIL
                     PEYPLKKDGREIPLHFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCGLYSSKI
                     SSNHVSFHLQPQNSVLSSLQLAS"
     gene            complement(1939..2136)
                     /locus_tag="VW411_001885"
     CDS             complement(1939..2136)
                     /locus_tag="VW411_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794548.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            complement(2140..2934)
                     /locus_tag="VW411_001886"
     CDS             complement(2140..2934)
                     /locus_tag="VW411_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794549.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEVPKELTPSEQLTWIAFLLTKGQKVVDVDLLSQVTGLPNSTI
                     YRCLRTFKALYWLNKQNKLYTYTVSKKELFLKSVSCLFNPIKKRILLPDGDIKQIKSV
                     SNLLYGGAYALSHSTFLAETDENISYVIWQRKFNQLSLPLSQHVLK"
     gene            complement(3342..3767)
                     /locus_tag="VW411_001887"
     CDS             complement(3342..3767)
                     /locus_tag="VW411_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794550.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYLGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVAKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEGASISLA
                     KKHNGILGSNNLRDVKSYVEEFSLEYMTTGDILIEAFKA"
     gene            complement(3896..4255)
                     /locus_tag="VW411_001888"
     CDS             complement(3896..4255)
                     /locus_tag="VW411_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794551.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKKEMALG"
     gene            complement(4301..4507)
                     /locus_tag="VW411_001889"
     CDS             complement(4301..4507)
                     /locus_tag="VW411_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794552.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMPLGR"
     gene            complement(4494..4655)
                     /locus_tag="VW411_001890"
     CDS             complement(4494..4655)
                     /locus_tag="VW411_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9794553.1"
                     /translation="MYVINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            complement(5013..5969)
                     /gene="lytA"
                     /locus_tag="VW411_001891"
     CDS             complement(5013..5969)
                     /gene="lytA"
                     /locus_tag="VW411_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEU9794554.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(6345..7715)
                     /locus_tag="VW411_001892"
     CDS             complement(6345..7715)
                     /locus_tag="VW411_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU9794555.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSTFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
BASE COUNT         2548 a         1605 c         1261 g         2475 t
ORIGIN      
        1 atatcactgt gaactattgt catgatatga agttgttcaa aatgagttgc agaaatatcg
       61 gacaagctgg taaaatcttg gctgacagtg gttatcaagg gctcatgaag atatgtcctc
      121 aagcacaaac tccacgtaaa tccagcaaac tcaagccgct aacagctgaa gataaagcct
      181 ataaccatgc gctatctaag gagagaagca aggttgagaa catctttgcc aaagtagaaa
      241 cgtttaaaat gttttcaaca acctatcgaa atcatcgtaa acgctttgga ttacgaatga
      301 atttgattgc tggtattatc aatcatgaac taggattcta gttttgcagg aagtctatta
      361 tatatgcaca gacatatgaa atctttttga gatagcagtt atgatatatt tatccattca
      421 caacatcttc tgtaccaata ctacaatatt ggtacatttt gattacacaa tcgtttcaaa
      481 aaatatgatg gaagcctgag tattttatag taactccctt cctatcaact agatcaacca
      541 gttgagtgtt gaatgttgaa acaccctagc ctcagcaaag gactatgagc agcatttcct
      601 agctaatagg acgctccaaa tcacctattc taaatccatc actagcttgg tcagtaagat
      661 acagactgca aggcaagact gacgtggttg atgagatttt ggaagagtat tacaatcata
      721 gattcaccag tagacttgac cgcagattta actatagtag attgaaacta gaatagtaca
      781 cttctgcttc taaaacattg ttagaaatcg atttgactgt cctgatcaat ttgtcctgtt
      841 ctcgtttcat tctactatac taaaaaacca cctgaaacaa atcaagtgac tagaggataa
      901 aaacgaagac tgggcaaaaa gttttgaaaa gcaaaaaacg cataatatca ggtcttgaaa
      961 aaccttgata ctatacgttt gattgtggga agattgactt cattttctcc tgaaattgaa
     1021 gttttttcta gtttccagat atagacgaat tcaatatagc catctcgcta caattatttt
     1081 aaccatcgag aaaatttttc agaatttcaa aaatttcatt ttgatccaat gaaatgtcat
     1141 tattttgaag aatagttgac ttcacaggct ctaattctat cacaaagcgg tgcatgtctg
     1201 cttttaccgc actacttgat gttatagccg ataacttttc atctcctagc aaggaagctg
     1261 tcaaacgggt aaggtcattc aaatgctttt taatggactt acttaaacct tgagcacctg
     1321 tggcagagcg ctccctcata tccagccaag ctttagcctt aaaaataatt aatactcaat
     1381 gaaaatcaaa gagcaaacta ggaagctagc tgcaggctgc tcaaaacact gttttgaggt
     1441 tgtagatgga agctgacgtg gtttgaagag attttcgaag agtataaacc acaattactc
     1501 aataccgaat acccctgaat ggtttctttt tcatgcacca atatattata ataatcttca
     1561 tccaataata aggctgataa actagcatct tggtcaaaat gtaagggaat ttctcgaccg
     1621 tccttcttta atggatattc tggtaagata ctaaatagtt caatcataga aggaaactca
     1681 ggattagttg ttgtaaatcg aaaaagctgc gctttctcat ctttctgact tccttgatac
     1741 tctcccaatt ctaaaaaatg attcaaggta gtataaaatt ccttattttt tacttcatca
     1801 atgatgacca tatcataatc ttttgttgtg cgacttttaa atccttgcga atccaatacg
     1861 atagaggtag cagttccccc aatcagaaca taatagttct gaaaatccgc aaacatttct
     1921 tgaaaaataa ctttcgtatt agctggcatc atcttctccc agatactgta atatcatatt
     1981 ttctagtgct tcactctctt cctctatacg tgggtcatca tcatctttta aggtcaaata
     2041 aagagaaatc ggatctacaa attgtttatc atgattattt ttaaaatcat tccaaaactc
     2101 agatacaaaa ggacgatatt tccatatctc tagcatcttt cattttaaaa catgctgaga
     2161 aagtggcaag gataactgat tgaattttct ctgccatatg acatagctaa tattttcatc
     2221 cgtttcagct aaaaaagttg aatgcgacaa agcataagca ccaccatata gaaggttaga
     2281 aacagatttt atctgcttta tatcgccatc tggcaataaa atccgttttt tgatgggatt
     2341 aaataaacat gacacggatt ttaagaataa ttctttcttt gacaccgtat atgtgtaaag
     2401 cttattttgc ttgtttaacc aatataaagc tttaaaagtc ctcaaacacc tataaattgt
     2461 tgagtttgga agtccagtga cttgtgaaag caaatcaaca tctactactt tttgaccttt
     2521 tgtcaataaa aaggcaatcc acgttaattg ttcgctaggt gttaattcct tagggacttc
     2581 agtatcattc gcattgagta ctagtcccaa tggagggaag aagaggtttc ccttaaagtc
     2641 tacaaacgga actctagctt gaagtaattg ctttttttca ctgtctgata acttcgaaaa
     2701 caccaaaaca acatccatat tggctttttc acccatagtg cgagcctgag taacaaatga
     2761 actcaaactc ccccttctct tttctttaat caataaaaat gattccttat gaaaagtcat
     2821 ttgagtatac tcaaaccttt ttacaaaact gattggtaaa gtcaaagtca gttcctcaat
     2881 actgacatca acataaaagg attgaaaaat tgacattatc tttttgacat tcatgttttt
     2941 tccttattct atcattttta ttatatttta tcattcggta taataaaata taagtttttt
     3001 gataaaataa ccaaaaacta ctcgctatcc cctctcacat taccctacat atcgtttgac
     3061 atgcgactga tagttcagga aaacttcaag gagaactttt ctctcatcca ctatgcagga
     3121 cttactattt cacttctact cctataggct caatttgaca cttttctttt tcgaattgct
     3181 catatgcctc ctgaatagtg agttcgattt atccaaattt atttttgtct attttgatga
     3241 atacttccac gaagatagtc tgaaaatgaa tttgcaccaa ttttccttct ctttttaagc
     3301 atattattcc agatatgatt gccctcttat tcagtaataa attacgcttt aaacgcttca
     3361 atcagtatat ctcctgttgt catatattct aaagaaaatt cttctacata tgatttaaca
     3421 tctcttaggt tattacttcc taatatccca ttatgctttt tcgctaagga aatagatgcc
     3481 ccttctccct taccaataat cttgttacta tcatgatttc ttgttaaatc tctatataat
     3541 gcgtattctt cagttccaat gtctatgctc acaatctcag ctgaaccctt agctaccaac
     3601 tgatctatcc tagattttaa atggggaatt gtaggtatat tgatttcatc atacacctct
     3661 tgtggaataa caattttacc caaatagagc ttttctaaaa gatgttcagt gccaacccat
     3721 aaaaatactg aaatacaatc tgcatcaata aatacacgac tagtcaaacg acaactcccc
     3781 cctcttcatc tagcccatat acaatatcat atctgaaagc atctagtaac agttcctcat
     3841 acttcccttg cgaaattctg ttattttcta aaagttgttc agttgaatta atatattatc
     3901 ctaatgccat ttctttttta ctttctgaca aaggacgata taaacttgta tcatagccta
     3961 atcttgaagc tgtctctata acactaatat ccatattttt aatttcttct gcatcaaggt
     4021 atccatcatt cctcaatcta tataacatag ctttatgact gataccataa aactgaccca
     4081 attttataat atcttctact tcaagatgag ttctattggc attttctctg atttcctcaa
     4141 ccatcctata cagtgaagat gggaaaatta aaaaataaga agcaaactga tccgcttttc
     4201 tttcagtttc atctccttca ccaatcaaga taagactgac tgaactcttc ttcacctcat
     4261 cataataaag atgatacagt ccatgtgcta aagaaaatct ttaccttcct aatggcatgt
     4321 ctgaattgac tgcaatgaga ctgaactgag ttcctttata acagactccg ctaatattct
     4381 ttccgagtcc ataaaatacc agcgtcaaat tttctatctt ttgtaccaat ttaaaaatat
     4441 ctatcggcga ttcaccatca gctcccaact tttttctaag atttgaagct ttattactta
     4501 aatccattcg tgatattcct ttcacctttt tccattttgg caatcatgct ttgattcaaa
     4561 gacaaatact cagcaatctt atcttgagta agatttgaag atattctaag ttcttttatt
     4621 ctttttccaa catcacatac atttatcaca tacatttatc atattcatac acctctcata
     4681 aaaagaatag cacactcttg tcataatttt ttaaataaaa aaattatgac aaaacaagga
     4741 agcaatttat tgatgctgct taaaaatcta aaattgatga gttcctatca aaaatcgtat
     4801 cttcaacctc aaaacagtac ttaaagctat ccgactcggt ttacattgtc aaatttagat
     4861 tttatttgag cataactttc tagtttgctt tttgattttt gtttaatata gtagcaaaat
     4921 tgaaggaaat atctccacaa gaaaacgcat actattaagc tttttcaaga cctaataata
     4981 tgcgctgttc tgatttgaaa gacattccat tattatttta ctgtaatcaa gccatctggc
     5041 tctactgtga attctggctt gtctgccagt gttccgtctg gtttgaggta gtaccagcct
     5101 gttccgtccg ctgactggat aaaggcattt gataccatgg cgccttcttt agcgtctaag
     5161 tagtaccaag tgtccttgta cttgacccag cctgtcttca tggcaccttc ttcgttgaaa
     5221 tagtaccact tatcagcgat tttcttccag cctgtagcca tttcgcctga gttgtcgaac
     5281 cagtaccagt tgccgtctgt gtgcttcctc cagcggtctg caagcatata gcctgaactg
     5341 tcaaagtagt accaagtgcc attgattttc tcaaacttgt cttttggata agagccgtct
     5401 gaatgtacgt accagtagcc agtgtcattc ttctgccagc ctgtttcaat cgtcaagccg
     5461 ttctcaatat catgcttaaa ctgctcacgg ctaatgcccc atttagcaag atatggataa
     5521 gggtcaacgt ggtctgagtg gttgtttggt tggttattcg tgcaatactc gtgcgtttta
     5581 attccagcta aactccctgt atcaagcgtt ttcggcaaac ctgcttcatc tgctagattg
     5641 cgtaagagtt cgatataaag gcggtagtcc gtcatgaact cttctttggt tgaatggctt
     5701 tcaatcagtt caaccgctgc ataggtctca gcattccaac cgcccccaac gtcccaggca
     5761 ccattatcaa caggtcctac ctgcatgatg caaccgttcc caacaatgtg cgagaaaaaa
     5821 cctaattctg ggtctttccg ccagtgataa tccgcttcat tctgtacggt tgaatgcgga
     5881 ttcccagttg agtgtgcgtg tacttgccta tatggttgca cgccgacttg aggcaaatct
     5941 gttcttaatt tactcacatt aatttccata ttctactcct tatcaattaa aacaactcat
     6001 tttttacaat ccagaaccag aaactccttt atttctacct tacaaagaag acaatcttag
     6061 tcacgattag gcttgtagat agaacttcaa aacgcactat tttgacactg taaataggac
     6121 tgacaaggtc tgcattctat ctacaataac accccagact aaaaagattt tcaaaagtat
     6181 atttttacag tctctatatg tccttttcat aaatactata ctttattata tcatataaaa
     6241 gaagtcaaaa gtctgttaaa ctattttcaa caccaaacta aagaagagaa cacaagtttt
     6301 ttcgattttc actagaggaa atggatttta ttcagtaaat ccaactagga ttgcactttg
     6361 gttgccaaga ttgccttgcc ttcttttatc aagggatgac ggaataatga gaagtacagt
     6421 tgaactgtca tggcaaccca gataagaggt tcacaaagga taacaccctt atatcctgcc
     6481 caaggaataa tcaaaaccac aaaaacgatt tttccgatta gttcaataaa gctagaaact
     6541 agaggaagga tcttttgccc caagccctgc aagcaattgc gataaatcaa caagaggctc
     6601 aaaatgggat aaaaggttga actgatttgc agatagagac ttccattttc tatcaagtaa
     6661 ccatctgtcg aactagccaa gaaggaaacc aaagctggac tggcaaaaaa gaggaaaata
     6721 caaacaaaaa ctgcccagga tatacttaaa cgactgccga ttcgaagacc ttgaacaata
     6781 cggtcaggtc gcttagctcc tagattctga gaagcaaagg tcgtcattga tgcagaaata
     6841 gcggtcatag gaagaagggc aaaggtcata atgcgtcgag ctgccgtctg ggcactaata
     6901 atcactgcac caaatgtatt aacagaaaac tgtaaaatca cactgccgat agatacaatt
     6961 gaactcatca agcccatagc caaaccttgc tccaagagat ccgcgtacaa gcttttgtcc
     7021 catttgaaat gtttaaactg tggcaagagt tctggcacac ttttacgaat ataataaaag
     7081 cagagaaccg ctgataaacc ttgcgaaata atggtagcaa gtcctgcgga ttgaactccc
     7141 agatgcaatt gcgtaataaa atagagatcc agaaccacat taaccaaggc agagaaaatc
     7201 agaaatccca gggctgctag actgtcacca atagaccgca acaagcctgc aaaaagatta
     7261 taagcaaagc tgacacctac acaggtcaca atcatagaaa tatattgata agattgagga
     7321 agaatttctg caggagtatc taagtattgc aagagaggat acaagccaag aaagcccagc
     7381 aacataacta gaatgctcaa aagagcacct aaaatccagg tggctgctac tgcttccttg
     7441 attttagtga aattccgagc cccataataa cgagcaatga caatccccat gccattgcca
     7501 acaccaagtg taaaacctac aatcaggtca aaaatcgctg tcgtcgctcc tactgcagcc
     7561 aaggattctt gaccaagaaa tcgtccaaca atcaagacat cagcagtgtt atagagctgt
     7621 tgaaaaatat ttgatagcaa aattggaaag gtgaagctta agagcgaggg aagaatcgga
     7681 ccatgtatca ggtccactgt tcgtttttta ttcataaagc tattatatca gctttccaga
     7741 ggagcgacaa gaaacagcaa actatatata gcggaatgaa ataaggaaag tcaaatcgat
     7801 ttttaacaat gttttagaag tagatatgta ctattctagt ttcgatctat tatagttggt
     7861 tgagtttgga atagtacgct gtagctgct
//
LOCUS       DAVKNL010000079         7885 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVKNL010000079 DAVKNL010000000
VERSION     DAVKNL010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7885
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.079"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            42..6464
                     /locus_tag="VW411_001893"
     CDS             42..6464
                     /locus_tag="VW411_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU9794556.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALNISDKEKV
                     AENKEKHENIHSAMETSQDFKEKKTAVIKEKEVVSKNPVIDNNTSNEEAKIKEENSNK
                     SQGDYTDSFVNKNTENPKKEDKVVYIAEFKDKESGEKAIKELSSLKNTKVLYTYDRIF
                     NGSAIETTPDNLDKIKQIEGISSVERAQKVQPMMNHARKEIGVEEAIDYLKSINAPFG
                     KNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKIPHAF
                     NYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQIFSY
                     KMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRALRKAG
                     IPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQTVEFD
                     KVNIGGESFKYRNIGAFFDKSKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDLRGKI
                     AVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGTKSQV
                     FSISGDDGVKLWNMINPDKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVGDEKE
                     IDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVINGKST
                     YGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQNTAR
                     PMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGSISLK
                     EIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGKQIVP
                     EIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESVEEME
                     ALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGKPKIP
                     GTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNEGINAPSSSGSKIA
                     NIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVISREHF
                     IRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATKIRGQ
                     FEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLITKDTY
                     HKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYAGEGQ
                     GRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANQADEKGMISY
                     YLVDPDQDSSKYQKLGEIAESKFKNLGNGKEGSLKKDTTGVEHHHQENEESIKEKSSF
                     TIDRNISTIRDFENKDLKKLIKKKFREVDDFTSETGKRMEEYDYKYDDKGNIIAYDDG
                     TDLEYETEKLDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNNYKSIEIKAT
                     KYDFHSKTMTFDLYANINDIVDGLAFAGDMRLFVKDNDQKKAEIKIRMPEKIKETKSE
                     YPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKGYALK
                     VTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRALSETTIYADSRNVEDGRSTQSVL
                     MSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKFNVE
                     AIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNWELRV
                     NESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMDGNVY
                     LQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKRNNGF
                     DGEIQEQHIYINGKEYTSFNDIKQIIDKTLNIKIVVKDFARNTTVKEFILNKDTGEVS
                     ELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKKDAGY
                     VINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDLQREE
                     HSQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIVGIFL
                     GLKKKNQD"
     gene            complement(6760..7106)
                     /locus_tag="VW411_001894"
                     /pseudo
     CDS             complement(6760..7106)
                     /locus_tag="VW411_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7201..7386)
                     /locus_tag="VW411_001895"
                     /pseudo
     CDS             complement(7201..7386)
                     /locus_tag="VW411_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            7414..7641
                     /locus_tag="VW411_001896"
     CDS             7414..7641
                     /locus_tag="VW411_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9794557.1"
                     /translation="MRERLLTQPLLHADETSYRVLESHSHLTYYWTFLSGKVENQAIT
                     LYHHDQSRSGSVVQEFLGDYSGYVHCDMLRQ"
     gene            7714..>7885
                     /locus_tag="VW411_001897"
     CDS             7714..>7885
                     /locus_tag="VW411_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9794558.1"
                     /translation="MVNCEPLEAYRQLEEAELVGCWAHVRRKFFEATPKQADKSSLGA
                     KGLAYCDQLFSLE"
BASE COUNT         2995 a         1104 c         1562 g         2224 t
ORIGIN      
        1 tattatctat tggaagttat ataaatatgt aaggaattaa aatgaaaaaa agtacagtat
       61 tgtcattaac cacagctgca gttattttag cagcctatgc ccctaatgag gtagtcttag
      121 cagacacatc tagctctgaa gatgctttaa acatctctga taaagaaaaa gtagcagaaa
      181 ataaagagaa acatgaaaat atccatagtg ctatggaaac ttcacaggat tttaaagaga
      241 agaaaacagc agtcattaag gaaaaagaag ttgttagtaa aaatcctgtg atagacaata
      301 acactagcaa tgaagaagca aaaatcaaag aagaaaattc caataaatcc caaggagatt
      361 atacggactc atttgtgaat aaaaacacag aaaatcccaa aaaagaagat aaagttgtct
      421 atattgctga atttaaagat aaagaatctg gagaaaaagc aatcaaggaa ctatccagtc
      481 ttaagaatac aaaagtttta tatacttatg atagaatttt taacggtagt gccatagaaa
      541 caactccaga taacttggac aaaattaaac aaatagaagg tatttcatcg gttgaaaggg
      601 cacaaaaagt ccaacccatg atgaatcatg ccagaaagga aattggagtt gaggaagcta
      661 ttgattacct aaagtctatc aatgctccgt ttgggaaaaa ttttgatggt agaggtatgg
      721 tcatttcaaa tatcgatact ggaacagatt atagacataa ggctatgaga atcgatgatg
      781 atgccaaagc ctcaatgaga tttaaaaaag aagacttaaa aggcactgat aaaaattatt
      841 ggttgagtga taaaatccct catgcgttca attattataa tggtggcaaa atcactgtag
      901 aaaaatatga tgatggaagg gattattttg acccacatgg gatgcatatt gcagggattc
      961 ttgctggaaa tgatactgaa caagacatca aaaactttaa cggcatagat ggaattgcac
     1021 ctaatgcaca aattttctct tacaaaatgt attctgacgc aggatctggg tttgcgggtg
     1081 atgaaacaat gtttcatgct attgaagatt ctatcaaaca caacgttgat gttgtttcgg
     1141 tatcatctgg ttttacagga acaggtcttg taggtgagaa atattggcaa gctattcggg
     1201 cattaagaaa agcaggcatt ccaatggttg tcgctacggg taactatgcg acttctgctt
     1261 caagttcttc atgggattta gtagcaaata atcatctgaa aatgaccgac actggaaatg
     1321 taacacgaac tgcagcacat gaagatgcga tagcggtcgc ttctgctaaa aatcaaacag
     1381 ttgagtttga taaagttaac ataggtggag aaagttttaa atacagaaat ataggggcct
     1441 ttttcgataa gagtaaaatc acaacaaatg aagatggaac aaaagctcct agtaaattaa
     1501 aatttgtata tataggcaag gggcaagacc aagatttgat aggtttggat cttaggggca
     1561 aaattgcagt aatggataga atttatacaa aggatttaaa aaatgctttt aaaaaagcta
     1621 tggataaggg tgcacgcgcc attatggttg taaatactgt aaattactac aatagagata
     1681 attggacaga gcttccagct atgggatatg aagcggatga aggtactaaa agtcaagtgt
     1741 tttcaatttc aggagatgat ggtgtaaagc tatggaacat gattaatcct gataaaaaaa
     1801 ctgaagtcaa aagaaataat aaagaagatt ttaaagataa attggagcaa tactatccaa
     1861 ttgatatgga aagttttaat tccaacaaac cgaatgtagg tgacgaaaaa gagattgact
     1921 ttaagtttgc acctgacaca gacaaagaac tctataaaga agatatcatc gttccagcag
     1981 gatctacatc ttgggggcca agaatagatt tacttttaaa acccgatgtt tcagcacctg
     2041 gtaaaaatat taaatccacg cttaatgtta ttaatggcaa atcaacttat ggctatatgt
     2101 caggaactag tatggcgact ccaatcgtgg cagcttctac tgttttgatt agaccgaaat
     2161 taaaggaaat gcttgaaaga cctgtattga aaaatcttaa gggagatgac aaaatagatc
     2221 ttacaagtct tacaaaaatt gccctacaaa atactgcgcg acctatgatg gatgcaactt
     2281 cttggaaaga aaaaagtcaa tactttgcat cacctagaca acagggagca ggcctaatta
     2341 atgtggccaa tgctttgaga aatgaagttg tagcaacttt caaaaacact gattctaaag
     2401 gtttggtaaa ctcatatggt tccatttctc ttaaagaaat aaaaggtgat aaaaaatact
     2461 ttacaatcaa gcttcacaat acatcaaaca gacctttgac ttttaaagtt tcagcatcag
     2521 cgataactac agattctcta actgacagat taaaacttga tgaaacatat aaagatgaaa
     2581 aatctccaga tggtaagcaa attgttccag aaattcaccc agaaaaagtc aaaggagcaa
     2641 atatcacatt tgagcatgat actttcacta taggcgcaaa ttctagcttt gatttgaatg
     2701 cggttataaa tgttggagag gccaaaaaca aaaataaatt tgtagaatca tttattcatt
     2761 ttgagtcagt ggaagaaatg gaagctctaa actccaacgg gaagaaaata aacttccaac
     2821 cttctttgtc gatgcctcta atgggatttg ctgggaattg gaaccacgaa ccaatccttg
     2881 ataaatgggc ttgggaagaa gggtcaagat caaaaacact gggaggttat gatgatgatg
     2941 gtaaaccgaa aattccagga accttaaata agggaattgg tggagaacat ggtatagata
     3001 aatttaatcc agcaggagtt atacaaaata gaaaagataa aaatacaaca tccctggatc
     3061 aaaatccaga attatttgct ttcaataacg aagggatcaa cgctccatca tcaagtggtt
     3121 ctaagattgc taacatttat cctttagatt caaatggaaa tcctcaagat gctcaacttg
     3181 aaagaggatt aacaccttct ccacttgtat taagaagtgc agaagaagga ttgatttcaa
     3241 tagtaaatac aaataaagag ggagaaaatc aaagagactt aaaagtcatt tcgagagaac
     3301 actttattag aggaatttta aattctaaaa gcaatgatgc aaagggaatc aaatcatcta
     3361 aactaaaagt ttggggtgac ttgaagtggg atggactcat ctataatcct agaggtagag
     3421 aagaaaatgc accagaaagt aaggataatc aagatcctgc tactaagata agaggtcaat
     3481 ttgaaccgat tgcggaaggt caatatttct ataaatttaa atatagatta actaaagatt
     3541 acccatggca ggtttcctat attcctgtaa aaattgataa caccgcccct aagattgttt
     3601 cggttgattt ttcaaatcct gaaaaaatta agttgattac aaaggatact tatcataagg
     3661 taaaagatca gtataagaat gaaacgctat ttgcgagaga tcaaaaagaa catcctgaaa
     3721 aatttgacga gattgcgaac gaagtttggt atgctggcgc cgctcttgtt aatgaagatg
     3781 gagaggttga aaaaaatctt gaagtaactt acgcaggtga gggtcaagga agaaatagaa
     3841 aacttgataa agacggaaat accatttatg aaattaaagg tgcgggagat ttaaggggaa
     3901 aaatcattga agtcattgca ttagatggtt ctagcaattt cacaaagatt catagaatta
     3961 aatttgctaa tcaggctgat gaaaagggga tgatttccta ttatctagta gatcctgatc
     4021 aagattcatc taaatatcaa aagcttggcg agattgcaga atctaaattt aaaaatttag
     4081 gaaatggaaa agagggtagt ctaaaaaaag atacaactgg ggtagaacat catcatcaag
     4141 aaaatgaaga gtctattaaa gaaaaatcta gttttactat tgatagaaat atttcaacaa
     4201 ttagagactt tgaaaataaa gacttaaaga aactcattaa aaagaaattt agagaagttg
     4261 atgattttac aagtgaaact ggtaagagaa tggaggaata cgattataaa tacgatgata
     4321 aaggaaatat aatagcctac gatgatggga ctgatctaga atatgaaact gagaaacttg
     4381 acgaaatcaa atcaaaaatt tatggtgttc taagtccgtc taaagatgga cactttgaaa
     4441 ttcttggaaa gataagtaat gtttctaaaa atgccaaggt atattatggg aataactata
     4501 aatctataga aatcaaagcg accaagtatg atttccactc aaaaacgatg acatttgatc
     4561 tatacgctaa tattaatgat attgtggatg gattagcttt tgcaggagat atgagattat
     4621 ttgttaaaga taatgatcag aaaaaagctg aaattaaaat tagaatgcct gaaaaaatta
     4681 aggaaactaa atcagaatat ccctatgtat caagttatgg gaatgtcata gaattagggg
     4741 aaggagatct ttcaaaaaac aaaccagaca atttaactaa aatggaatct ggtaaaatct
     4801 attctgattc agaaaaacaa caatatctgt taaaggataa tatcattcta agaaaaggct
     4861 atgcactaaa agtgactacc tataatcctg gaaaaacgga tatgttagaa ggaaatggag
     4921 tctatagcaa ggaagatata gcaaaaatac aaaaggccaa tcctaatcta agagcccttt
     4981 cagaaacaac aatttatgct gatagtagaa atgttgaaga tggaagaagt acccaatctg
     5041 tattaatgtc ggctttggac ggctttaaca ttataaggta tcaagtgttt acatttaaaa
     5101 tgaacgataa aggggaagct atcgataaag acggaaatct tgtgacagat tcttctaaac
     5161 ttgtattatt tggtaaggat gataaagaat acactggaga ggataagttc aatgtagaag
     5221 ctataaaaga agatggctcc atgttattta ttgataccaa accagtaaac ctttcaatgg
     5281 ataagaacta ctttaatcca tctaaatcta ataaaattta tgtacgaaat ccagaatttt
     5341 atttaagagg taagatttct gataagggtg gttttaactg ggaattgaga gttaatgaat
     5401 cggttgtaga taattattta atctacggag atttacacat tgataacact agagatttta
     5461 atattaagct gaatgttaaa gacggtgaca tcatggactg gggaatgaaa gactataaag
     5521 caaacggatt tccagataag gtaacagata tggatggaaa tgtttatctt caaactggct
     5581 atagcgattt gaatgctaaa gcagttggag tccactatca gtttttatat gataatgtta
     5641 aacccgaagt aaacattgat cctaagggaa atactagtat cgaatatgct gatggaaaat
     5701 ctgtagtctt taacatcaat gataaaagaa ataatggatt cgatggtgag attcaagaac
     5761 aacatattta tataaatgga aaagaatata catcatttaa tgatattaaa caaataatag
     5821 acaagacact aaacattaag attgttgtaa aagattttgc aagaaataca accgtaaaag
     5881 aattcatttt aaataaagat acgggagagg taagtgaatt aaaacctcat agggtaactg
     5941 tgaccattca aaatggaaaa gaaatgagtt caacgatagt gtcggaagaa gattttattt
     6001 tacctgttta taagggtgaa ttagaaaaag gataccaatt tgatggttgg gaaatttctg
     6061 gtttcgaagg taaaaaagac gctggctatg ttattaatct atcaaaagat acctttataa
     6121 aacctgtatt caagaaaata gaggagaaaa aggaggaaga aaataaacct acttttgatg
     6181 tatcgaaaaa gaaagataac ccacaagtaa accatagtca attaaatgaa agtcacagaa
     6241 aagaggattt acaaagagaa gagcattcac aaaaatctga ttcaactaag gatgttacag
     6301 ctacagttct tgataaaaac aatatcagta gtaaatcaac tactaacaat cctaataagt
     6361 tgccaaaaac tggaacagca agcggagccc agacactatt agctgccgga ataatgttta
     6421 tagtaggaat ttttcttgga ttgaagaaaa aaaatcaaga ttaagataaa agctatagaa
     6481 aaaaatggtt tatgtactga gattagatag tgaggtgatg acatagtttt gtgaaaatag
     6541 ccatttataa ctcaattatt tagtttactt tactttacta gtgatactat ttggagttat
     6601 taatggactt agtttatata actaatgaat tgattgaaag ggttagtatt gacaatattg
     6661 gtcatattga ctagaaaata gagtctatca aaatttaaag gctaatagag gtgatgagac
     6721 aatttcggct ctttgtcaac tgtagtgggt tgaagtcagc taagctcgag aaaggacaaa
     6781 ttttgtcctt tcttttttga tattcagagc gataaaaatc cgttttttga agttttcaaa
     6841 gtttcgaaaa ccaaaggcat tgcgcttgat aagtttgatg agattattgg tcgcttccag
     6901 tttggcatta gaatagtgta gttgaagggc attgacaatc ttctctttat ctttgaggaa
     6961 ggttttagag gatgaacttg attcagattg tcctcaatga gtccgaaaaa tttgtcaggc
     7021 tccttattct gaaagtgaaa aagcaagagt tgatagagat tatagtggtg tttcaagtct
     7081 tctgaatagc tcaaaagttt atctatagta gattgaaact agaatagtac acctctgctt
     7141 ctaaaacatt gttagaaatc gatttgactg tcctgaatga tttgtcctgt tattatttca
     7201 ttttactata aatccacgtt tacgaatctc tttccacact tgttcaatgg ggttcatctc
     7261 tggtgtgtat ggaggaataa atgcaaaacc aatattagtc ggaatcttta aggtacttga
     7321 tttatgccat atagcattgt ccataacgag taaaagataa tcatctggat aagcttgtga
     7381 aagctcctat tcctaaagcc cctttataac ctcttgcgag agagactatt gactcagccc
     7441 ttacttcatg cggatgaaac ttcttatcgg gttctagaga gtcatagcca tctgacctac
     7501 tattggacct ttttgtctgg gaaagttgag aatcaagcaa tcacgctgta ccatcatgat
     7561 cagagtcgga gtggttcggt agtacaagaa ttcctaggag attattctgg ctatgttcat
     7621 tgtgatatgt tgcggcagta acttaggact ttagtcctct agttctgcct atgcgatagc
     7681 agtccaaggt ttaggagcaa ggcgacgcta agcttggtaa actgcgaacc gctagaagct
     7741 tatcgtcaac tggaagaagc tgaacttgtt ggatgttggg cgcatgtgag aaggaaattt
     7801 tttgaagcga cccccaagca agcagataaa tcatccttag gagctaaagg tttagcttat
     7861 tgtgatcagt tattttcctt ggaaa
//
LOCUS       DAVKNL010000080         7658 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVKNL010000080 DAVKNL010000000
VERSION     DAVKNL010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7658
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.080"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(203..1171)
                     /locus_tag="VW411_001898"
     CDS             complement(203..1171)
                     /locus_tag="VW411_001898"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU9794559.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(1316..1939)
                     /locus_tag="VW411_001899"
     CDS             complement(1316..1939)
                     /locus_tag="VW411_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEU9794560.1"
                     /translation="MPKKVLILSLLGGMFLSGWLSSFANTYIHDLLGVLFPDSPFLNA
                     FESAIAAPLVEEPLKLLSLVFVLALIPVRKLKSLFLLGIASGLGFQMIKDIGYIRTDL
                     PEGFDFTISRILERIISGIASHWTFSGLAVVGVYLLYRAYKGQKVGKKQGLIFLGLAL
                     GTHFLFNSPFVELETELPLAIPVVTAIALYGFYHAYCFVEKHNELMT"
     gene            complement(1921..2130)
                     /locus_tag="VW411_001900"
     CDS             complement(1921..2130)
                     /locus_tag="VW411_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794561.1"
                     /translation="MKKSNILFIFILLLCIGLQYETIYYTDGSRSGAEYGLMGVSIFL
                     ALFYMIPALYFLFRIGKNGNCQRRF"
     gene            complement(2155..2652)
                     /locus_tag="VW411_001901"
     CDS             complement(2155..2652)
                     /locus_tag="VW411_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEU9794562.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(2725..4086)
                     /gene="radA"
                     /locus_tag="VW411_001902"
     CDS             complement(2725..4086)
                     /gene="radA"
                     /locus_tag="VW411_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEU9794563.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(4100..4615)
                     /locus_tag="VW411_001903"
     CDS             complement(4100..4615)
                     /locus_tag="VW411_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9794564.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(4617..5060)
                     /locus_tag="VW411_001904"
     CDS             complement(4617..5060)
                     /locus_tag="VW411_001904"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEU9794565.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            complement(5117..5214)
                     /gene="ffs"
                     /locus_tag="VW411_001905"
     ncRNA           complement(5117..5214)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VW411_001905"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5247..5714)
                     /locus_tag="VW411_001906"
     CDS             complement(5247..5714)
                     /locus_tag="VW411_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEU9794566.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYNILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(5915..7201)
                     /locus_tag="VW411_001907"
     CDS             complement(5915..7201)
                     /locus_tag="VW411_001907"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEU9794567.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(7432..7644)
                     /locus_tag="VW411_001908"
     CDS             complement(7432..7644)
                     /locus_tag="VW411_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HEU9794568.1"
                     /translation="MANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVRYGIGCRKD
                     FIVYHYRVAYRLYLEKLVMNRGFISC"
BASE COUNT         2432 a         1880 c         1240 g         2106 t
ORIGIN      
        1 agaattctcc ttagaaagcg tcgattcaag tgctatcact gttcaaaaat gatggtcgct
       61 gaaacttctg atgacgtaca gtcatatttc ttctcttttt attatatcac agttttaaat
      121 ctagctttac tagattcacc gctactatct atttattcgg aaaaaagacg aaaaaacctg
      181 agaatcatct caggcttggt cattaaattt ttttctcaat atcgaaaagt ggagaaagtg
      241 gtcgtttttc atgaatacgt acgatagcat cccctaggag atgagcgatt gaaatctgct
      301 caatcttatc aatcaaacgc tcttctggca gatagatggt atccaaaaca accaatttct
      361 taatagctga tttttggata ttgtccgtag caggaccaga aagaactggg tgcgtacagc
      421 ttgcatagac ttcaacagca ccagcttccg caagagcatc tgccgcatga caaatcgttc
      481 cagcggtatc aatcatatca tcaatcaaga tacaagtctt gccttcaacc ttaccgatga
      541 tattcataac ttcactagta ttcatcttat caacgctacg acgtttatca ataatagcga
      601 tagatgtttt caaaaattct gccaacttac gagcacgagt cacccctcca tggtccgggc
      661 tgacaaccac atagtcagaa ccaaccatac cacgacgctc aaaataatct gcaatcagag
      721 gagcacccat caaatgatcc acaggaatat caaagaatcc ttgaatttgc gcagcatgca
      781 agtcgatggt caataaacga tccactccag ctacttcaag catatttgcg acaagttttg
      841 aagtgattgg ctcacgcgct ctcgcctttc tatcctgacg tgcataccca tagtaaggca
      901 tgacaacatt gacagattct gcactcgcac gcttcaaagc atctaccata atcaaaattt
      961 caagcagatt gtcatttaca ggcgaactag ttgattgtaa gataaagacg tgtttcccac
     1021 ggattgattc ttcaatgttg acctgaatct ctccatctga aaattggcga acacttgatt
     1081 tccccaactc tatcccaatc tcctgcgcca cacgttctgc caattcttta ttagaagaaa
     1141 gggcaaacag ctttaaatca gaaaaagaca tgatttcctc cggtatatat gtataacttg
     1201 tgcttttcac aagattttcc atctaccatt gtagcgcttt ttgcactatt tttcaatcaa
     1261 aaataaaaga agggcaccat atttgtaccc ttgcatcatt cttttgaaaa atattctagg
     1321 tcatcaactc attgtgtttc tcaacaaagc aataagcatg ataaaaacca tagagagcaa
     1381 tagccgtaac cactggaatc gctaaaggca actctgtttc caactccaca aaaggagagt
     1441 taaacaagaa gtgagttccc aaggctaaac ctagaaaaat aaggccctgt ttcttgccaa
     1501 ccttctgtcc tttataggct ctgtaaagca agtaaacacc tactacagct agacctgaaa
     1561 aagtccagtg agaggcaatt cctgagatga tacgctctaa aattcgcgaa atagtaaagt
     1621 caaagccctc tggcaaatcc gtacgaatat aaccaatatc cttaatcatt tggaatccca
     1681 aaccggaagc aattccaagt aaaaacaaag attttaattt tcgcacagga atcaaagcca
     1741 aaacaaaaac aagtgacaat aatttcaagg gttcttctac caaaggagcc gcaatagcac
     1801 tttcaaaggc atttaaaaat ggactatctg ggaaaagaac ccccagtaaa tcatggatat
     1861 aagtattagc aaaactagac aaccagcctg aaaggaacat ccctcccaat aaagacagaa
     1921 tcaaaacctt ctttggcaat tcccattttt cccaatacgg aagagaaaat aaagagccgg
     1981 aatcatgtaa aagagagcta gaaagataga aactcccatt agtccatatt ccgcacctga
     2041 cctcgaaccg tccgtatagt agatggtttc atactgtaaa ccaatacata gcaataaaat
     2101 aaaaataaat aaaatattgc ttttcttcat acactttctt tctaaatgaa gtatttataa
     2161 ttctacgact gtcatacttc ctgtatcaac attgtaaatg gcaccagaga taatgacatc
     2221 gtctggtatt aggggagact cgataagcag ttgcatatcc tcgcgtacac tctcttctat
     2281 atcttggaag ggcaagaagt cctggtctga cacatcgaca cccaattctt ccttcaaata
     2341 ctcctgaaaa ggttcatttt caaaggtctg agcaccacag tctgtatgat gcaataccac
     2401 aatttctctt gtccccattt gttgctggga aataactaga gaacgaatca tatcctcagt
     2461 cactcgacca cctgcattcc gcaaaatatg agcatcccca agtgccaaac ctagagcttg
     2521 cgcaacgtgc aaacgtgagt ccatacaggt cacaatggct actctggttt taggtttaag
     2581 tggcagattt aactgcccat gtagggcaac ataagcctga ttggcttgca taaactgttc
     2641 aaaatacgac acgattccct ccttgaaaat ttgatagtca aatatttctc ctatcttatc
     2701 atttttaaga gaatttgtca cggattatgc aaagaccttt ttcaagactt cctgaatcgt
     2761 tgtcacgcca atgacctgaa tttccttagg cagagtgatt cctgtcaagg aattcttagg
     2821 tacataaatc ttagtaaagc ccagtttagc agcttcgttg atgcgttgct caatacgatt
     2881 cacgcgccga atctctcctg tcaagcccag ttctccgaca aaacattcct gaggattagt
     2941 tggcttgtct ttgtagctcg aagcaatagc aactgcaaca gccaagtcaa tcgcaggttc
     3001 atccaattta acaccaccag cagatttgag ataggcatcc tgattttgca agagaagccc
     3061 tgcccgtttt tccaaaacag ccataatcaa gctagcacgg ttaaaatcaa gtcctgtcgt
     3121 agtacgcttg gcatttccaa acatggtcgg tgttaccaaa gcctgaacct ccgccaaaat
     3181 cggacgcgtc ccttccatgg ttacaacgat ggaggaacca gtcgccccat ccaaacgctc
     3241 ttctaggaaa acttgactcg gattgagtac ctcaaccaag ccgcccgact gcatctcaaa
     3301 aatcccaatc tcattagtgg aaccaaaacg atttttgacc gctctcaaaa tacgaaaggt
     3361 gtggtgacgc tccccttcaa agtaaagcac cgtatccacc atatgctcca acatacgagg
     3421 cccagccaag gttccttctt tggtcacatg acctacgata aagatggcaa tgttattggt
     3481 cttggccaac tgcatgagtt cagcggtcac ttcacgcacc tgagaaacag acccctgcac
     3541 ccctgaaatc tcaggagaca tgatggtctg gatggaatca ataatgagaa agtctggctg
     3601 gatacgctcc acttctgcac gaacactctg catattggtc tctgcataga gataaaactc
     3661 actatcaata tcacctaagc gctctgcacg tagtttaatc tgctgggcag actcctcccc
     3721 actgacatag agaactgtcc ccacttggga caactgggtt gagacttgta ggagaagagt
     3781 tgatttccca atcccaggat ccccaccgat aaggacgaga cttcctggta ccactccgcc
     3841 tccaagcaca cggttgaatt cctccatctc cgtcttggtt cgattgacat tgatggaagt
     3901 cacctcagct agtttcatgg gcttggtttt ctcacctgtc aaggacacac gcgcattctt
     3961 aacttcggca acctcaacct cttccacaaa agaagaccaa gacccacagt tggggcaacg
     4021 tcccagatat ttaggggaat tatacccaca attttgacat acaaatgtcg cttttttctt
     4081 tgcgatgaca aacctctttc tatatctcta actcacactc aatcacttgg caaaaatcaa
     4141 tcttctcatt tggcacaaac tggcgcatga gcattcgatg agcaacaact accacagtct
     4201 gatgttctcg atacttagac atacattcta gaaaccgaga cttcatttcc gtagctgtct
     4261 catattgaat aggactatta ggaagcaact cccccttgtt ttctaaaaac agtcttctag
     4321 ctgtttcaaa gttttctatt cctgttttat agacctgcca ttcatgtaat aaaggctcta
     4381 ctcttaaagg aagacccgta gcacagacca catacgaagc cgtttctaaa gctcttgtga
     4441 ctgcagaaga tacgattatt tcagctgacg agagtaaagg atttttgctc aatttctgga
     4501 cttgctgccg tcccatctca gacaagggtg ccaaatctat cccaaatcct atataagaac
     4561 gctcctctaa ctcacggtaa tctggctccc catgacgtac aaagataatc ttcattctag
     4621 tgccctgtcg atccaaatcc accagttcga acgccatcag ctgcatctcc atctgcaatt
     4681 aagaaagtag caaaaacagc ctggacaata cgctccccaa cttcaagaac aacctcttgg
     4741 tctgtgatat tcttcatctg cgcaaaaata tgcccttcat ttccaggatt tccataataa
     4801 tccccatcaa tgactccaac tgagttaatt aaaaccaagc ccttcttacg aggatttgaa
     4861 gaacgatcat agaggtagag aacctcagtc ggctgcatat aagccttaac ccctgtcgga
     4921 accaagacaa tctctcctgg cgcaacaact gtacgcacag caacctttaa gtcgtaacca
     4981 gccgcatgcg ctgtctcacg cttgggcaat aaattttcat ctgtaaaact cgaaaccaat
     5041 tcaaaaccac gaattttcat aattttctct tttctattat catttattct agattattct
     5101 atcttattta ttcggaaaaa gcacgaaaaa aagagcacac aattcaaatc gcttagggct
     5161 gctggattcc tcccctgacc cgcttcacgc agaactgttg ctccactatt tattatatca
     5221 cattcctatt cattttaaaa gcaaaattat tttttccgtc tatttctaaa aaaatcctgc
     5281 atgatagctg cgcattcatc ttccaaaatt cctatttcaa cctccacacg atgattgaga
     5341 cgctcatctg tcaagatatt gtacaaactc ccagcagcgc caaatttctg gtttttagcc
     5401 ccatagacca catttggaat gcgggcaagc ccaatcgctc cactacacat gacacaaggt
     5461 tcaatggtca caaaaagtgt gcaatccagc aagcgccagc tctcctcact caagttcgca
     5521 tcctctatag ccataatttc cgcatgcata accgctcgct gtaattcctc acgcgcatta
     5581 tgcccacgac caatgatttc cccatctttg acaatcacac aaccaattgg aatttcatcg
     5641 tgttcaagag caatctcagc ctctctcaaa gcctccctca taaagacttc tttttcttca
     5701 agcgtataat acatctcttt tctcttttcc tacttatcga ttttattatt atatcatgaa
     5761 tcccaacaca aaaaaagcca ccgaatgcgg tgactctata gggagattat tatgaaaaag
     5821 aaaagtttag gatatttgtt acaacaagtt aggaggtctt cttgtaaccg tctatagtat
     5881 acccgaccta tcttaaacaa atcttaaaaa tctcttagga ccaaacactt tctaaaatat
     5941 ttgtttgttc acgaccagga cctactgaga aagtagaaat acgaacgcca accaattcac
     6001 tcacacgacg aacatagtta cgcgcattct caggaagatc ttccaaattg cgaactccgg
     6061 taatatcttc tgaccaacct ggcaactctt catagatagg cttgcaacgt ttcaattgtt
     6121 caagactagc tggatagtag tcaatacgtt gaccgtcaag atcataggcc acacagattt
     6181 tcacagtatc caaaccgctc aaaacatcaa tagagttcaa agaaaggtta gtaataccag
     6241 aaacacgacg gctatgacgc atcacaactg agtcaaacca acctacacga cgtggacgac
     6301 cagttgttgt accatattca tgacccactt cacggatacg ttctcccact tcatcaaaca
     6361 actcagttgg gaaaggacca tctcctacac gactcgtata agctttacat acacctacaa
     6421 ccttgtcaat cttgcttgga ccgacaccag aaccaattgt cacaccacca gctacagggt
     6481 ttgatgacgt aacaaatgga taagtacctt ggtcgatatc tagcataaca ccttgtgcac
     6541 cttcaaaaag cacacgtttg ccattatcaa gcgcatcatt caagataaca gatgtatcta
     6601 tcacgtattt cttgatttgt tgaccatatt cgtaatattc ttcaaaaata tcatcgaaaa
     6661 caatcgcttt actgtcatac aatttttcaa aaagacgatt cttttcagca aggttacgtt
     6721 ctaaacgctc acggaaaata tctttatcta aaagatctgc aatacgaatt ccaacacgag
     6781 cagccttgtc catataagct ggaccaattc ccttaattgt cgtaccaatc ttattgtcgc
     6841 ccttagcttc ttcttgcaag cgatccaact cgatatgata aggcaaaata acatgcgcac
     6901 gatcagaaat acgcaagtta tcagttgtta caccttcctc atgaagatag ctcaactctt
     6961 ttacaagaga tttaggattt acaaccatac cattcccaat gacagatatt ttttcaggga
     7021 agaaaatccc agatggaatc aagtgcaact taaatttctt accgtcaatc acaatcgtgt
     7081 gaccagcatt atcaccacct tggtaacgtg caatcacttc tgcattcgct gaaaggaagt
     7141 ctgtaatctt ccctttacct tcatcacccc attgggtacc tacaacaaca actgaagtca
     7201 taatcttgtc tgagccatca ggctcttcct ttctcacata catggcagga ctctcacctg
     7261 caattatatc ttacaattta ttataataaa aaatcgcctt tttatcaaga agaaacaata
     7321 gaaagatttg ctatttccaa ctattaaaaa atgatttaga aaaattacta gctatttact
     7381 atcatctttc cataaaagag taaattagtt cggaaattta ctaaaattac ctcaacaaga
     7441 aataaaaccc cgattcatta ccaatttttc aagatacaaa cgataagcaa cacgataatg
     7501 gtaaacaata aaatccttac gacaaccaat gccatatctc actaaataat aaattaaaaa
     7561 tttaaaatga acatgttccc agtcaaaatt atcaccaaat gtaggaccat actcttcttc
     7621 aatactatca tagaaattag ccatctgctc ataaattt
//
LOCUS       DAVKNL010000081         7561 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVKNL010000081 DAVKNL010000000
VERSION     DAVKNL010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7561
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.081"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(72..1238)
                     /gene="recA"
                     /locus_tag="VW411_001909"
     CDS             complement(72..1238)
                     /gene="recA"
                     /locus_tag="VW411_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEU9794569.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVPLDLGDELEIEIEE"
     gene            complement(1293..2549)
                     /locus_tag="VW411_001910"
     CDS             complement(1293..2549)
                     /locus_tag="VW411_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEU9794570.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FTLRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            complement(2634..3650)
                     /locus_tag="VW411_001911"
     CDS             complement(2634..3650)
                     /locus_tag="VW411_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU9794571.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSIGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNLLRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            complement(3658..4176)
                     /locus_tag="VW411_001912"
     CDS             complement(3658..4176)
                     /locus_tag="VW411_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9794572.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            complement(4166..4609)
                     /gene="tsaE"
                     /locus_tag="VW411_001913"
     CDS             complement(4166..4609)
                     /gene="tsaE"
                     /locus_tag="VW411_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU9794573.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            complement(4695..5315)
                     /locus_tag="VW411_001914"
     CDS             complement(4695..5315)
                     /locus_tag="VW411_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794574.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            complement(5698..6561)
                     /locus_tag="VW411_001915"
     CDS             complement(5698..6561)
                     /locus_tag="VW411_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9794575.1"
                     /translation="MNTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEVKSNLSNFKHLSARLLDDRNYDDLEYLYKIEVERS
                     TFLTLEDRTYLEWIKSIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELDTV"
     gene            7026..7250
                     /gene="pneA1"
                     /locus_tag="VW411_001916"
     CDS             7026..7250
                     /gene="pneA1"
                     /locus_tag="VW411_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA1"
                     /protein_id="HEU9794576.1"
                     /translation="MTNFNSNEKFCGKSLKSLSADEMSLIYGASDGAEPRWTPTPIIL
                     KSAAASSKVCISAAVSGIGGLVSYNNDCLG"
     gene            7257..7445
                     /gene="pneA2"
                     /locus_tag="VW411_001917"
     CDS             7257..7445
                     /gene="pneA2"
                     /locus_tag="VW411_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA2"
                     /protein_id="HEU9794577.1"
                     /translation="MKNDFVIGKSLKELSLEEMQLVYGGTDGADPRSTIICSATLSFI
                     ASYLGSAQTRCGKDNKKK"
BASE COUNT         2293 a         1600 c         1264 g         2404 t
ORIGIN      
        1 ctactatata taatctaaaa acttgaatca aaaatcgaaa aagtagcgga tttctccact
       61 gctttaacag cttattcttc aatttcgatt tcaagttcat cgcctaagtc aagcggaact
      121 tcttcattca ctgcttcttc tttctttggc tcatcttttt tgttttcagt atcttgttct
      181 gaaacttctt ctccatcaat caagccaaat ttagaacgga cttgcttatc aatttcatca
      241 aagatttctg ggtgctctgc caagtatttc ttagcattct cagaaccttg cccaattttt
      301 tcatctttgt aagaatacca agcccctgct tttttgataa tatccaaatc gcttgcaatc
      361 ttcaaaagct caccagtctt agaaattcct tctccgtaca taatttcaac tacggcttcc
      421 ttaaacggtg gagctacctt attttttaca accttaatct tagtttcttt accgacattg
      481 gtttcttttt ggtcaccagt tcccttaatt tgtgtattac cacgaacatc caagcggact
      541 gaagcataga atttcaaagc acgtccgccc ggtgttgttt ctggatttcc aaacatcact
      601 ccaacttttt cacgcaattg gttgataaaa atggcaattg ttttggtttt attgatagag
      661 gcgccaagtt tacgcatggc ctggctcatc atacgagcct gcaaaccaac atggctatct
      721 ccgatatctc catcaatttc cgcacgagga acaagggcag caactgagtc gactacgaca
      781 agatcaactg cacctgagtc aatcaatttt cccgcaatct caagaccttg ctctcctgag
      841 tctggttgag acaagagcaa ttcgtcaata ttgacaccaa gggccgcagc ataagctgga
      901 tcaagggcat gttccgcatc gataaaggca gcaatcccac cttctttttg cgcttgtgca
      961 actgcatgaa gggcaaccgt tgtcttacca gatgactctg ggccatagat ttcgatgata
     1021 cgtcccttag gataaccacc tgagccaagg gcaatgtcaa gagctaaaga acctgagctc
     1081 atcacttgca ccttttgctc cgcacgttca cccaaacgca tgattgatcc tttaccaaag
     1141 tctttctcaa tcaatttaag agcgtcattc aaggcctttt cacgttctgc cccaaatttt
     1201 tttgaaattt cttctaattt ttttggtttt ttcgccattc tattctccta cattctaatg
     1261 atcctcagac ctatctacta ttatatcaaa agttagtcac ttaataaagc cttgcgaact
     1321 aggttaaagg catgcataac cgcaatgtga cgtacatctg ctcggcttct gcctccaata
     1381 ttaaccttga taacctcagt tccttgatct tgcgccaagc ctatgaagac tgtcccaact
     1441 gggtgtcctt ctaggctatc tggtcctgcc actccagtca aactaattcc aaaatcagac
     1501 tgggtcttgc ttcgtgcctg ctcagccatc ttctgagctg taaattcaga caccacacca
     1561 tgttcttcca aattcttggc aggaatatcc aacatccttg atttttcctc caagctatag
     1621 gtcacaaaac cacccttaaa tatacttgaa actccagaaa aattcgccac ggtagcttgg
     1681 aaaagacctg ccgtcaaact ctctgcagcc gcgatggttt tcccttgcct tttcagttct
     1741 tctaccacaa tgctggctaa actagtttct tccccataac catagcaaaa gtctcgtaaa
     1801 gaaattcctt cgaaagtctg gcagtccaag atttgatttt ccaagatatc cagcgcttga
     1861 ttcgcctctt cttgactgct agcctttgtt gacagacgta gagtgacttc tcctgtcttg
     1921 gcataagggg ccaaggtagg atcgatctga ttatcaatta aatcagccaa aatcgtaacc
     1981 aactggctct cgccaatccc aaagaaacga agaactcggg aatacagctt gctccctgtc
     2041 atcaacttgg gtagaagttg gtttaagacc atgggtttca attcacttgg cggacctgga
     2101 aggacgacat aggtcactcc gtctacttct aattttcctc caacagccag tcctgtttcg
     2161 tttggcagtg gaatcgctcc ttctacaatt tgagcttgtc tttcgttatt cggtgttcgg
     2221 gcatagtctg gtcgcagggt aaaaaagata tccaacttct cctgagcctg aggatcaaag
     2281 actaatgctt tccctaaaaa tttagctagg gtttgtttgg ttaggtcgtc ctcagttggc
     2341 cccaaaccgc ctgtcaaaat caccagactg ctacgttgac tggcaatctc aagcaaagac
     2401 aagagacgaa cttcattgtc tcctacagcc gtctgaaaat atacatctac cccaatctca
     2461 gctagttttt ccgacaaaaa ctgggcattg gtgttgacaa tctgccctgt caaaatctct
     2521 gttccaacag caatgatttc tgctttcatg tttcctccta cctatctatt cgtatttttt
     2581 tgaaaaaatc gcaggaattt tcctacgatt gattttttta tttgtatcaa aagttaatta
     2641 tcttcatcac caacaggtgc tctgccaaat aaatcttcaa ataaaaccgc attggtttca
     2701 agctgagtaa cttcttcttg tcccaaagaa cgtcggagta gattttgcat ttccaacata
     2761 tgtgctctcg aaacaatctg gtaagaaaca ccttgaagta tctctccttc accctgcaac
     2821 tgctgagttt caatggtttt aaatgaatct ttatagccta gcaagttagg gatacttttt
     2881 gcagacaaat caatattggt ctgcatattg tcactcaaag cttttagaat ctcttgataa
     2941 tgaccaatgc tatttaaact gagagctttt tccatgactt tttgaataac ttcacgttga
     3001 cgtttttgac gaccataatc cccctcagga tcttggtaac gcattcgtgc atagactagg
     3061 gcttcttctc ccccaatatg ttgctcccca acaccgatag aaatagtatt aaattcttct
     3121 tggtcactga tagaaattgg gaaacctagg atattattga ctgtaatacc tcctactgca
     3181 tccactagtt tttgcaatcc tctcatattg accatcacat agcgatcaat atggatattc
     3241 atcatttttt gaatggtttc tatagcaagc tctgctccac catctgcata tgctgagttc
     3301 agtttcgctt catgagcctg accattccct gattcaatgc gcgtcagaat atcccgctct
     3361 aaactcatca ttgttgtttt tttcgtttta ggattcactg tcatcaagat catgctatca
     3421 cttctaccga cccaagtttc agttcgttca acatttccgg tgtccactcc cattaacaga
     3481 atggttagag gttcagtcgc ttcaataacc ttggtttctt caccgatttt tttataggtt
     3541 ttagctaagg tttctgtccc ttgttgataa atagtataag caaaaacacc tactcctact
     3601 acagttacag aaagtaaagc tagcaccatt ccaataattt ttttaaccat atttctacta
     3661 acctatcagt ttacccatca agtaaacatc gataaatttc ccttcttcta tatatgcccc
     3721 acgctcttgg ctaccttcaa tgacaaagcc atgcttttga taaagatgga ctgctgcttg
     3781 attacgagtt tggacagtca gttggagacg acgcagaatg ccacttgctt gtgcccactc
     3841 tatcgcttct tctagcaaca aacttcccaa gccattattc caatatcttt ttccaatcac
     3901 aatgaagaga tctccaatat gacggactct cttacgctga tcagctgtaa tatttacaat
     3961 accagcaatt ttgccattta agaatgcaag taaggttatc tgattgtccg aactagcttg
     4021 cttgttgagg aatatttcca tctcctcact agtcaagaga ataccatctc cgtctaggct
     4081 ggtaaagtct gtctccaaac tcacacgatt taaaaaggcc actaattcag ctgcatcttt
     4141 gggctctgct tccctaatga gcaattcata ctccatattg aagctcctct aacaatttct
     4201 cagcacgcaa accctttgcc tgaaaattta aacggcgtcc atctgcttct tttagaattt
     4261 ccaattctaa ataagcatct ggcaaggcat ctcctaagag atttccccac tcaataacag
     4321 tcacgccgcc accaaagata aactcatcca agtcgataga atcagcatct ccttcaatac
     4381 gataaacatc taggtgataa agtggaagtc gaccttcata ctctctcacg atagtatagg
     4441 tgggactttt aatcatttga gaaatctgta atccttttgc aagtccttta gtaaaggtcg
     4501 ttttacctgc acccagttct ccagttaaga ttaaaacatc attctttgct aatagatggc
     4561 ccaaacgctc ccctaaggct tgcaactctt cttcattttt tgtgtacata ctcttattat
     4621 accaaaaact tttcttttgt gtctattttc ctactaaact tatcatcata acatccataa
     4681 aaaacaggct ttctctaaaa gaaaatgagc gtaacaatga ccaatacaag atctcggaaa
     4741 atatgaccat aaaaggaaac ttccttctta accgaatttg ggacaagata ggctgcaaaa
     4801 aacaagccca gtccaatata aatcagaagt gagacaatgg tcattggatt tcttaagaaa
     4861 agaagtgttg ctaaaatagt caccaacact gtcttttttc tgtccagcat agcaagaaaa
     4921 tcgcgcacgt attttttcaa gggtaaaaaa atcagcaaat ctagcccaaa taggaaaaag
     4981 aaggatggca ataaaaagtc aactaattct tgctgcagcg tatttttgat gaacaagtta
     5041 tctgacaaaa caagaacagc tcctaacaaa ttaattaaga gtaacatact gtaaaaaagc
     5101 ttcaccgact tcttactggc taggacacta tggacttctt gcttacgggt ataaagataa
     5161 tttactccag cacagattcc tgaaacgaaa accatgcttc cgatgaaaaa agctgtactt
     5221 tgtttaaagg acaagatgca ttccttccat aggaaacagc tactcaaact gatttgaatt
     5281 aaagctaaca aaaataagat tctcattgat ttcatcttct ctctcccttc ctaccaatca
     5341 ttatactagg agaaaagaga gaactgtttc taatcttctc aaatgtctct ttaagacgct
     5401 aaacaaacac tagagactaa tactcaatga aaatcaaaga tcaaactagg tagctagcca
     5461 caggttgctc aaaacagtgt tttgagattg cagatagagc tgacgtgatt tgaagagatt
     5521 ttcgaagaat ataaatttga aatcatgaaa atccgtcaaa cgggtggttg ttttgtctcg
     5581 cacctcacgg agcgagacgg actcagagtc acataattat aaggctgata gtattaatct
     5641 aactatcagc cttacaggtt atttaacgtt tcagaaaaac tataatgtca agattaacta
     5701 aacagtatct agttccttca aataattttc tatcttcatc aacattaaag gattgttata
     5761 aatcttacat aactctcttg cttctatata ataatttttg acttgttctc tgtctagaaa
     5821 tttggctcca gcatttccta caagaataag tagaggagcc aattggtagc ttgtctgtct
     5881 ttgtttacag agttcaatcg tttcaagagc ttcttggatg gcttcattat atttttcctt
     5941 tgatactagg tagtgagcgt agttgtaacg aactctgatg tagccaaata aaaactcttg
     6001 atggtccaaa ttttttgtct gatacaactc tattaaatga gagtagtttg cctcatattc
     6061 ttgttcacga cccactaagg aatagaaatt agatagagta ttcaacgcct ttaaataaat
     6121 cagagtattt gaagagactt ttaataatat attttccaat gacgaaattg cctcacactt
     6181 actgtcatat tgatagaagt caattataga tttaatccat tcaaggtaag ttcggtcttc
     6241 taatgttaga aaagtgcttc gttctacttc tattttataa agatattcta aatcgtcata
     6301 atttctgtca tctaataggc gagcagatag atgtttgaaa ttagagaggt tagacttaac
     6361 ttcgatttgt tcattgaaaa agtaatccaa agggacttca agtcgttgag agagtttgaa
     6421 taacaagtct gcggagggaa taaaatgacc tctttcaatt ttactaatct ggctttgttc
     6481 acaaattcct tctgcaagag tttgttggga gagtctcaac tcttttcttt tcaatctgaa
     6541 tttctctgcg agtgtattca ttaaagataa taaaccttcc tatttgctta atttcattat
     6601 aaagttttta gttcaaaata gcaagttaaa ggaataaata attctttatg gtaataaaca
     6661 aaaaaaaaag ttaaaaatta cataaaaata cttgactaaa taaaatatat ctgttagaat
     6721 aaaaacaagg aaaaagaaag gggtttcatt gcatgagaaa aaaacgtgga attaaaaaat
     6781 tagttacatt tgcattgcta ggtgttttta tgtttagtaa tacaattcct taccaacagt
     6841 ttattcagaa gaataaacaa ttggagattc gagtgcaatc gcaaaagaag tccaatggtc
     6901 ttgatgttgg gaaggcggat taaaacccga tattttgcac gagaatgaga gaattataat
     6961 ttatctgaaa aaacgaaaaa tatagagcaa cttatttaac tttttataag gaggagttat
     7021 tacttatgac caactttaat tcaaacgaaa aattttgtgg aaaaagttta aaatcattgt
     7081 ctgctgatga aatgagttta atttatggtg catctgatgg tgcagagcca cgttggacgc
     7141 ctactcctat tattttgaaa tcagctgcag ctagctcgaa agtatgcatt agtgcagctg
     7201 tttctggtat tggggggcta gttagctaca ataatgattg tttggggtaa attaaaatga
     7261 aaaatgattt tgtaatcgga aagagtttga aagaattatc tcttgaagaa atgcaattgg
     7321 tttatggtgg cacagatggt gctgacccta gatcaacaat tatatgttct gctacattaa
     7381 gttttattgc atcatatttg gggtcagctc agactcgttg tggcaaagac aacaagaaaa
     7441 aataaaatat tcaattttaa aaataaaagt tgctctttat tttcctctct cgtaactaat
     7501 aattttgaga gaggtttttt tttaaaacaa tagtaagtcg tataggtttg aggtaagtaa
     7561 a
//
LOCUS       DAVKNL010000082         6926 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVKNL010000082 DAVKNL010000000
VERSION     DAVKNL010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6926
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.082"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            469..1830
                     /gene="trpE"
                     /locus_tag="VW411_001918"
     CDS             469..1830
                     /gene="trpE"
                     /locus_tag="VW411_001918"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEU9794578.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETTSVQVTKYMEVELFRY
                     VMHLTSVVKGRLLPELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            1827..2393
                     /locus_tag="VW411_001919"
     CDS             1827..2393
                     /locus_tag="VW411_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEU9794579.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEEVVK"
     gene            2404..3408
                     /gene="trpD"
                     /locus_tag="VW411_001920"
     CDS             2404..3408
                     /gene="trpD"
                     /locus_tag="VW411_001920"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU9794580.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            3405..4172
                     /gene="trpC"
                     /locus_tag="VW411_001921"
     CDS             3405..4172
                     /gene="trpC"
                     /locus_tag="VW411_001921"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEU9794581.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            4159..4758
                     /locus_tag="VW411_001922"
     CDS             4159..4758
                     /locus_tag="VW411_001922"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEU9794582.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQPFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            4736..5959
                     /gene="trpB"
                     /locus_tag="VW411_001923"
     CDS             4736..5959
                     /gene="trpB"
                     /locus_tag="VW411_001923"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU9794583.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGRDLPDALVACVGGGSNAIGLFHPFVEDESVAMYGTEAAGLGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            5952..6728
                     /locus_tag="VW411_001924"
     CDS             5952..6728
                     /locus_tag="VW411_001924"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEU9794584.1"
                     /translation="MPKTLTEKLNAIKAAGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLANTDIALIPLVSLTTGI
                     ERQKELIEGAEGFIYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIRQAVAYQK"
     gene            complement(6700..6801)
                     /locus_tag="VW411_001925"
                     /pseudo
     CDS             complement(6700..6801)
                     /locus_tag="VW411_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
BASE COUNT         1990 a         1391 c         1726 g         1819 t
ORIGIN      
        1 agatagaact gacgaagtca gtaaccatac ctacggcaag gcgacgttga cgcggtttga
       61 agagattttc gaagagtata aaggtgtacc gagatgaaca tctcttcatc aatgtctctg
      121 gtcgtggagg caaggaccta gctgcgattg cagactacta gaagctaaaa aataatagat
      181 ggaaaaatta cagttctttc tctcacagag agcttgtggt tgctgaaaac aagcagagaa
      241 agctgtaaag ttgggttcat ctgaaacgag agaagaaaac atcattctca agggagtgcc
      301 ctttatcgca cagcctgtta caggagatat ctgagacagg aatgagagat aggagaaatc
      361 ctataattga ggtggcaccg cgaatttcgt cctcacgcaa gttattttgc gtggggattt
      421 ttcatacagt caccacggac tagaagtaga actgaaaggg aaattacaat ggaacgaatc
      481 attcatggag atgtcttatc accaatcttg gcttatatgc gcttaaaggg gcaacataag
      541 gttattttag agagtattcc gagagacaag gaaacagctc gtttttctat cctagcctat
      601 aatccagttt ttgagattaa gtttgaaaat ggggttctct atcaaaatgg tcaagtgatt
      661 gatcgggatc ccttggattt cctttatgaa gtgattcata agagtcagca ccactcagag
      721 ctaccttttg gtggtggagc cattggtttt gtgggttacg atatgatttc gctttatgaa
      781 gaaattggtc aaatccctga ggatacaatt gggacgccag acatgcattt ctttgtctat
      841 gagagctata tggtctttga ccacaagaag gagaagatac atgtcataga ggatgcactt
      901 tatagtgagc gcagtcaaga ggccttggaa aaatccttga accaagtgct tgaggaatta
      961 cgcattcctg ctccaaatga atttgaagat ttggatctat ctccgttaga cttcaaaccg
     1021 catatcgctc cacataagtt tgagggaatg gtagaaacag ctcgtgactt gattcgtaac
     1081 ggagatatgt tccaatgtgt actcagccag cgtttttcag cagaagttac tggaaatcca
     1141 tttgacttct acagaaatct ccgcgtgact aatccttcta attaccttta tttctatgat
     1201 tttggggatt atcaaatcat cggagctagt ccagaaagtt tggtttctgt caaaaatggc
     1261 atcgtgacaa ccaatccgat tgcagggacg cgaccaagag gggctacgga tgaagaagac
     1321 aaggccttgg cgactgacct cctctctgat gagaaggaaa cagcagaaca tcgaatgttg
     1381 gtagacttgg ggcgtaacga tattggccgc atctctgaaa cgaccagtgt ccaagtcact
     1441 aagtatatgg aagtggagct cttccgctac gtcatgcatt tgaccagcgt agtcaagggg
     1501 cgtttgcttc cagaactcac tgccatggat gccttgaaag caacgcttcc tgctggaacc
     1561 gtttctggag caccaaagat tcgagcgatg agacgtatct atgaactgga aacggaaaaa
     1621 cggggagtat acgctggggc aatcggctac ttgtctgcga cgggtgatat ggatttggcc
     1681 atcgccatcc gaaccatgat tctcaaaaat caaagagcct atgtgcaggc tggggcaggg
     1741 attgtctacg actctatcgc ccaaaacgaa taccaagaaa ccattaacaa agcaaaatct
     1801 atgactagaa ttggagaact aagaccatga ttttattgat tgacaactat gattctttta
     1861 cctataactt ggcccaatac attgggaatt ttgcagaagt tcaggttctg agaaatgatg
     1921 attccaagct gtatgaagaa gctgaaaaag cagatggtct ggtcttttct cctggtcctg
     1981 gttggccagt tgatgctgga aagatggaag acatgattcg tgattttgca ggcaagaagc
     2041 cgattcttgg gatttgtttg ggccaccaag ccattgcaga agtctttggt ggtaagctag
     2101 gtttggctcc aaaagtcatg catgggaaac agagcaatat caactttgaa gcgccatctg
     2161 ttttgtatca aggtattgag gatggccgtg cggtcatgcg ttatcacagt attttgattg
     2221 aggaaatgcc agaagacttt gaagtgacag ctcgttcgac tgatgaccaa gccatcatgg
     2281 ggattcaaca taaaaaccta ccgatttatg gcttccagta ccatccagag agcattggaa
     2341 cgccagacgg cttgtcttct attcggaatt ttatcgagga ggttgtaaag tgaggaaact
     2401 aggatgaaag agattattga aaaactagca aaatttgaaa atttatcagg tgtggaaatg
     2461 acggatgtca ttgagcgtat cgtaactggg cgtgtaactg aagcgcagat tgcttctctc
     2521 ctcttagctc ttaagatgaa gggggaaaca cctgaagaac gcacagccat tgcccaagtc
     2581 atgagaggac atgcccagca tattccaact gagattcatg atgccatgga caactgtggt
     2641 acaggtgggg acaagtcttt cagttttaat atttccacaa ctgcagcctt tgtcttggct
     2701 ggtggcggta ttcacatggc caaacacggt aaccgctcga tatcttctaa atctggttcc
     2761 gcagatgtcc tcgaagcctt gggaatcaat cttgacctca aaccagctga actaggtaag
     2821 gtctttgata aaactggaat cgtctttctc ttcgctaaaa atatgcaccc agctatgaaa
     2881 tacatcatgc cagctcgttt ggaattggga attccaacga ttatgaactt gactggtcca
     2941 ctgattcatc caatggcttt agaaacacag cttcttggaa ttagtcgtcc agaactccta
     3001 gaaagtacag ctcaggtttt gaaaaatatg ggtcgcaaac gtgccatcgt ggttgctgga
     3061 ccagaagggt tggatgaagc tggcttgaac ggaacaacca agattgcact tcttgaaaat
     3121 ggcgaaatca gcttgtcaag ctttactcca gaggatttgg gaatggaagg ctatgctatg
     3181 gaagatattc gtggtgggaa tgctcaggaa aatgcagaaa ttttgcttag cgttctgaaa
     3241 aacgaagcaa gtccattctt ggaaacgaca gtcttgaatg ctggtcttgg tttctatgct
     3301 aatggtaaga ttgatagcat caaggaaggg gttgccttgg cccgtcaagt gattgctaga
     3361 ggcaaggccc ttgaaaaact cagactgtta caggagtacc aaaaatgagt caggaatttt
     3421 tagcacgaat cttagagcag aaggcgcgtg aggtggagca gatgaagctg gagcaaatcc
     3481 agcctctgcg ccagacctat cgcttggcag aatttttgaa gaatcatcag gaccgcttgc
     3541 aggtaatcgc tgaggtcaag aaagctagcc ctagtttggg agatatcaat ctcgatgtgg
     3601 atattgtgca acaggcccag acttatgaag aaaacggagc agtgatgatt tcggtgttga
     3661 cagatgaggt tttctttaaa gggcatttgg attatctacg ggaaatttcc agtcaggtag
     3721 agattccgac gctcaacaaa gactttatca tcgatgaaaa gcaaatcatc cgcgctcgca
     3781 atgcaggtgc gacagttatc ttgctcattg tggcagcctt gtccgaagaa cgcctcaagg
     3841 aactgtatga ctacgcgaca gagcttggtc tggaagtctt agtggagact cacaatctag
     3901 ctgaactaga ggtagcccac agacttggtg ctgagattat cggggtcaac aaccgcaact
     3961 tgactacctt tgaggtcgac ttgcagacca gtgtagattt agccccttac tttgaggaag
     4021 gtcgctatta catttctgaa tctgccattt tcacagggca ggatgcggaa cgactagccc
     4081 catactttaa cggaattttg gtagggacag ctcttatgca ggcagaaaat gtggcccaga
     4141 gaatcaagga gttgcagatt gacaaaggtt aaaatttgtg gactatcgac caaagaagcg
     4201 gtggaaacag ccgtttcagc aggagccgac tatatcggtt ttgtctttgc acctagtaaa
     4261 agacaggtga ctttagaaga ggcagctgag ttggcaaagc ttattcctgc agatgtgaaa
     4321 aaggttggag tatttgtttc accaagtcgg gtagaactgc tggaagcgat tgacaaagtt
     4381 ggcttggact tggttcaagt tcacggtcag gtggcagatg atttatttga gaatttgcct
     4441 tgtgccagta ttcaggctgt gcaggtagat ggaaatgggc atgtccccaa ttctcaggca
     4501 gattatctac tctttgatgc ccctgtggca ggaagtggcc agccctttga ttggggtcaa
     4561 ctggatacga ctggactagc acagcccttc tttatcgcag gtggccttaa tgaagataat
     4621 gtagtaaaag caattcaaca ctttactccc tatgcagtag atgtatcgag cggagtggag
     4681 acagatggac aaaaagatca tgaaaagatt agaagattta tagagagggt aaagcatggc
     4741 atatcaggaa ccaaataaag atggatttta cggaaaattc ggcggacgtt ttgtcccaga
     4801 aacattgatg acagcagttt tggagttgga gaaggcctac cgtgaaagtc aggcagaccc
     4861 aagtttccaa gaggaattaa accagctttt gcgccagtac gtaggacgtg aaactcctct
     4921 ttattacgca aaaaacttga cccagcatat tggcggagcc aagatttatc tcaaacggga
     4981 agaccttaac catacaggag cccacaagat taacaatgcc ttaggacaag tttggcttgc
     5041 caaacgcatg ggtaaaaaga aaattatcgc agaaacgggt gctggtcagc acggtgtggc
     5101 aactgcaact gctgcagccc tctttaacat ggaatgtacc atctacatgg gtgaggaaga
     5161 tgtcaaacgc caagccctca atgtcttccg catggagctt ttgggagcca aggtcgaggc
     5221 tgtaacagat ggttcgcgcg tgctcaagga tgcggtcaat gcagcccttc gttcatgggt
     5281 ggctaatatc gacgataccc actatatcct tggttctgcc ttggggcctc atccatttcc
     5341 agaaatcgtt cgtgacttcc aaagtgtcat cggtcgagag gctaaacaac agtaccgtga
     5401 cttgacaggt cgagatctgc cagatgccct agtagcctgt gttggtggtg ggtcgaatgc
     5461 tatcgggctc ttccatccct ttgtagaaga tgagtcagta gctatgtatg gaactgaagc
     5521 agcagggctt ggtgtggata cagagcacca cgcagctacc ttgaccaagg gtcgtccagg
     5581 tgtccttcac ggttccctca tggatgtgct ccaagatgcc catggtcaaa ttcttgaagc
     5641 cttctctatc tcagcaggtt tggactatcc tggtatcggt ccagagcatt ctcactacca
     5701 cgatattaaa cgtgccagct atgtccctgt gacagacgaa gaagccttgg aaggattcca
     5761 actcttgtct cgtgtggaag ggattatccc agccttggaa tctagccatg ctatcgcctt
     5821 tgcggtgaaa ttggccaaag aacttggacc agaaaagtct atgattgtct gcctatcagg
     5881 tcgtggggac aaggatgtgg ttcaagtcaa ggaccgcttg gaagcagatg cagcaaagaa
     5941 gggagaagct catgcctaag acactaacag aaaaattaaa tgctattaaa gcagctggaa
     6001 aaggaatttt tgttccctat atcatggctg gtgaccacga gaaaggtttg gacggtcttg
     6061 ctgaaacaat tcacttttta gaagatttgg gtgtctctgc aattgaagtg ggtattccct
     6121 tttcagaccc tgttgcagat ggacctgtta tcgaagaagc tggcttgcgc agtttagccc
     6181 acgggacctc tacccaggct ttagttgaaa ccttgaaaac cattgaaaca gagattccac
     6241 tggtcatcat gacctacttc aaccccctct ttcagtacgg tgtggagaac tttgtcaaag
     6301 atctggctga tacagctgtg aagggcttga ttatcccaga cctgcctcat gaacatgcca
     6361 actttgtaga accatttttg gcaaatacag atatcgcctt gattccttta gtaagcttga
     6421 ccacaggaat tgagcgccag aaagagttga ttgaaggggc ggagggattc atctatgccg
     6481 ttgccatcaa tggagtgaca gggaaatcag gcaattaccg tgcagatttg gacaagcact
     6541 tggcgcaact tcatcaagtg gccgacatcc cagtcttgac aggttttggt gtatctagtc
     6601 aagccgatct ggaacgcttc aatgcggtgt cagatggtgt tatcgtcggt tcgaaaatag
     6661 taaaagctct ccaccaagga gagccgattc aggattttat cagacaagca gtagcttacc
     6721 aaaaataatc aaacaagtag caagtaagag gaaaggcacg aaaggaagtc tttccttttt
     6781 cttttgcagg agaaaggtca gttttatagt gaattgaatc tggaatatta cactacggtt
     6841 cctaaaatat tttcagaaag cgatttgaat ttcccaataa atttgttcag tttttaattg
     6901 attttactat agaagatata attttc
//
LOCUS       DAVKNL010000083         6817 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVKNL010000083 DAVKNL010000000
VERSION     DAVKNL010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6817
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.083"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            <1..384
                     /locus_tag="VW411_001926"
     CDS             <1..384
                     /locus_tag="VW411_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630-Spn1 family
                     transposase"
                     /protein_id="HEU9794585.1"
                     /translation="LIRGKVSGRRYQRISLVAGLTNGELIAPMTYEETMTSDFFEAWF
                     QNFLLPTLNTPSVIIMDNVRFHRMGKLELLCEEFGHKLLPLPPYSPEYNPIEKTWAHI
                     KKHLKKVLPSCNTFYEAFLSCSCFN"
     gene            445..1407
                     /locus_tag="VW411_001927"
     CDS             445..1407
                     /locus_tag="VW411_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU9794586.1"
                     /translation="MNMSNITIDGQEMDLSQTMPSYAQVVSLYDDTSISVSNNETDKV
                     LAGSLYTDTNEQGLTIPSSLLKNWNEQTGKNLTANDLIGKSVSASIVESAAETSKIAQ
                     FQTKIVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTE
                     ELQKNKKYTVLSQQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRS
                     KEIGVMKAVGYQNRDVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSIT
                     KVFELNLLSVLGTLVFALLLGYVSAYFPARKISKMDPIESLCYE"
     gene            1584..2591
                     /gene="gap"
                     /locus_tag="VW411_001928"
     CDS             1584..2591
                     /gene="gap"
                     /locus_tag="VW411_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU9794587.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFIKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2659..3495)
                     /locus_tag="VW411_001929"
     CDS             complement(2659..3495)
                     /locus_tag="VW411_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9794588.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWKEIVNPGD
                     VCQLTFDEEDYSQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLAHCKLKTGRTHQIR
                     VHLSHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKK
                     NG"
     gene            3621..5816
                     /locus_tag="VW411_001930"
     CDS             3621..5816
                     /locus_tag="VW411_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU9794589.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETKAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEASWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYVMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5869..6021
                     /gene="rpmG"
                     /locus_tag="VW411_001931"
     CDS             5869..6021
                     /gene="rpmG"
                     /locus_tag="VW411_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9794590.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNLKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            6031..6207
                     /gene="secE"
                     /locus_tag="VW411_001932"
     CDS             6031..6207
                     /gene="secE"
                     /locus_tag="VW411_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEU9794591.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            6262..6798
                     /gene="nusG"
                     /locus_tag="VW411_001933"
     CDS             6262..6798
                     /gene="nusG"
                     /locus_tag="VW411_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEU9794592.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
BASE COUNT         2122 a         1195 c         1473 g         2027 t
ORIGIN      
        1 ttaataagag gcaaagtatc tggaagaaga tatcagagga tttctttggt tgcaggtcta
       61 acaaatggtg aattaatcgc tccaatgact tacgaagaga cgatgacgag cgactttttt
      121 gaagcttggt ttcagaattt tctcttacca acattaaaca caccatcggt tattattatg
      181 gataatgtaa gattccatag aatggggaag ctagaacttt tatgcgaaga gtttgggcat
      241 aaacttttac ctcttcctcc ctactcgcct gagtacaatc ctattgagaa aacatgggct
      301 catatcaaaa agcacctcaa aaaggtatta ccaagttgca atacctttta cgaggctttt
      361 ttatcctgct cttgtttcaa ttgactatat atcacggata cagttgggaa agaagctatt
      421 aaatatttgg agagtccttt tgggatgaac atgtcaaata ttactataga tgggcaagaa
      481 atggatctga gtcaaacgat gccttcttat gctcaagtag tgagtcttta tgatgataca
      541 agtatctctg ttagtaataa tgagacggac aaagtgttgg ctggatccct ctatactgat
      601 acaaatgaac agggattaac gattccaagc agtttactaa aaaattggaa tgaacagaca
      661 ggaaaaaatt tgacagctaa tgatcttatt ggcaaatcag tctcagccag cattgtagaa
      721 agtgctgccg aaactagtaa gattgctcaa tttcaaacga agattgtacg tgtaatcaat
      781 gatgaagatg acatggagga cagcaacagt ttcatgctgt ctcatcaaat ggaaacgatt
      841 ttgaaagagg ctggatttac gaaagctgta tcttatttta tcttggaact caaagatcca
      901 tcacagacaa aagtagtaac agaagaatta cagaaaaata agaagtatac tgtgctttct
      961 caacagagag ttcttgatat tgtgattacc tttattcgtg ttattcaggg attattgatt
     1021 gtgctttcat cacaagctat tgtggtagca gcggttatga ttggtatcat tatttacatc
     1081 aatatcatgc aacgttccaa ggaaataggt gtcatgaaag cagttggtta tcagaatcgt
     1141 gatgtcaaag gaatttttat ttacgaggct atctggattg taggcatcgc cttgctgctg
     1201 gcatttttgg ttgcacaagg ggtgggaagt ttggcgaatg cgattgtaag tcacttttac
     1261 ccatccatca ctaaggtttt tgaattaaat cttttatctg ttttaggaac tctagttttc
     1321 gctctattac ttggttatgt ctcagcctac ttcccggcgc gtaagattag taaaatggat
     1381 cctatagaat cgttatgcta tgaatagtaa gggattcctt ggtttacagt cttaaaaaga
     1441 aactaacata gaaaaacaat ggccatactt gcaattcaac tcaaatagtt gtataatagg
     1501 tttgttgaaa ggtgatttgt agtgattcaa gttactcttt tcacaataaa aatttcatga
     1561 ttttcataag gaggaaatca ctaatggtag ttaaagttgg tattaacggt ttcggacgta
     1621 tcggtcgtct tgctttccgt cgtatccaaa acgtagaagg tgttgaagtt acacgcatca
     1681 acgaccttac agatccagtt atgcttgcac acttgttgaa atacgacaca actcaaggtc
     1741 gtttcgacgg tactgttgaa gttaaagaag gtggatttga agttaacggt aaattcatca
     1801 aagtttctgc tgaacgtgat ccagaacaaa tcgactgggc tactgacggt gtagaaatcg
     1861 ttcttgaagc tactggtttc tttgctaaga aagaagcagc tgaaaaacac cttaaaggtg
     1921 gagctaaaaa agttgttatc actgctcctg gtggaaacga cgttaaaaca gttgtattca
     1981 acactaacca cgacgttctt gacggtactg aaacagttat ctcaggtgct tcatgtacta
     2041 caaactgctt ggctccaatg gctaaagctc ttcaagacaa ctttggtgtt gttgaaggat
     2101 tgatgactac tatccacgct tacactggtg accaaatgat ccttgacgga ccacaccgtg
     2161 gtggtgacct tcgccgtgct cgcgctggtg ctgcaaacat cgttcctaac tcaactggtg
     2221 ctgcaaaagc tatcggtctt gtaatcccag aattgaatgg taaacttgac ggatctgcac
     2281 aacgcgttcc aactccaact ggatcagtta ctgaattggt agcagttctt gaaaagaacg
     2341 ttactgttga tgaagtgaac gcagctatga aagcagcttc aaacgaatca tacggttaca
     2401 cagaagatcc aatcgtatct tcagatatcg taggtatgtc ttacggttca ttgtttgacg
     2461 caactcaaac taaagttctt gacgttgacg gtaaacaatt ggttaaagtt gtatcatggt
     2521 acgacaacga aatgtcatac actgcacaac ttgttcgtac tcttgaatac ttcgcaaaaa
     2581 ttgctaaata attcttgagt tgatagaaag caaggctttg tggtcttgct tttttatatg
     2641 gaaaaatgga tgacacgatc atccattctt ttttaattct ttttcaaatg tatttgaaag
     2701 ggtagtgaaa gttagcttct ctaaagtaag tgggtgggta aaggaaagtc ggaaggcatg
     2761 aagcataagc cggcttgtct ttgatttact attatagaga gggtctccca ggataggaag
     2821 attatgatgc gaaaggtgca cacgaatctg atgggttcgc cctgtcttta gcttgcaatg
     2881 agccaaggaa gtcttgtttg agaattgctt taatctgctt acatgcgttt cagcatattg
     2941 cccatttttt gcatcaacta ttctttttct acgatcatgg cgatcacgtc caattttgtc
     3001 tctgaaaaca agttcttttc tgttgatatt tccatcaact agagcccaat attctctaga
     3061 aatctctttt ttctccaata agcgattgag aatgggcagg ataaaaggat ttttggcaaa
     3121 gagaactaag ccactggttt ccatgtccag acgatgaacg acatagcagg tttggccaac
     3181 ataggtactg acatggttaa gaagggcaat ttcgtttggt tgattaccat gcgttttcat
     3241 cccctctggt ttgtttacaa taatcaagtg ttgatcttga taaacttcct gcactaagtc
     3301 tgggttgccc caagggatcg tcttttggga ataatcttcc tcgtcaaaag tcaactggca
     3361 aacatctcca ggatttacga tttccttcca gtggacttct tcttgattta tcaaaatatg
     3421 tttcttgatt ctcaaaaaat gacggatttt tctagggatg aggagttgtt cctcaagtaa
     3481 ttgctttact gtcatttgag gtagagaggc gggtaatgta aatgtgaatt gcatacagat
     3541 attgtaacaa aaaaagccct atttggatag gaaatagcta aattcttgtc ttcctatgat
     3601 gaagatgata aaataaacgc atgaaattag ataaattatt tgagaaattt ctttctcttt
     3661 ttaaaaaaga aacaagtgaa ctagaggact ctgattctac tatcttacgt cgctctcgta
     3721 gtgatcgaaa aaaattagcc caagtaggtc cgattcgaaa attctggcgt cgttatcatc
     3781 taacaaagat tatccttata ctaggtttga gtgcaggctt gctagttgga atctatttgt
     3841 ttgctgtagc caagtcgacc aatgtcaatg atttgcaaaa tgccttgaaa actcggactc
     3901 ttatttttga ccgtgaagaa aaagaggctg gtgccttgtc tggtcaaaag ggaacctatg
     3961 ttgagctgac tgacatcagt aaaaacttgc agaatgctgt tattgcgaca gaagaccgtt
     4021 ctttctataa aaatgacggg attaactatg gccgtttttt cttggctatt gtcactgctg
     4081 gacgttcagg tggtggctct accattaccc aacagctggc taaaaacgcc tatttatcgc
     4141 aggatcaaac tgttgagaga aaagcgaaag aatttttcct tgccttagaa ttaagcaaaa
     4201 aatatagtaa ggagcaaatt ctaaccatgt accttaacaa cgcttatttt ggaaatggtg
     4261 tgtggggtgt agaagatgcg agtaagaaat actttggagt ttctgcatca gaagtgagtc
     4321 tggatcaagc tgcgactctg gcagggatgc tcaaggggcc ggaactgtat aatcccttga
     4381 attccgtaga agattctact aatcggcgcg atactgtctt gcagaatatg gttgcagcag
     4441 gatatattga taaaaaccaa gaaaccaaag ctgctgaagt tgatatgact tcgcaattgc
     4501 acgataagta tgaaggaaaa atctcagatt accgttaccc ctcttatttt gatgcggtgg
     4561 ttaatgaagc tgtttccaag tataatctaa cagaggaaga gattgtcaat aatggctacc
     4621 gcatttacac agagctggac caaaactacc aagcaaatat gcagattgtt tatgaaaaca
     4681 catcgctatt tccgagggca gaggatggaa cgtttgctca atcaggaagt gtagccctcg
     4741 aaccgaaaac agggggagtt cgtggagttg tcggtcaagt tgctgacaat gataaaactg
     4801 gattccggaa tttcaactat gcaacccaat caaagcgtag tcctggttct acaattaagc
     4861 ctttagttgt ttatacgcca gcagttgaag caagctgggc tttgaataag cagttggata
     4921 accataccat gcagtatgac agctataagg ttgataacta tgcagggatc aaaacgagtc
     4981 gagaagttcc tatgtatcaa gccttggcag aatcgcttaa tctacctgct gttgccactg
     5041 ttaatgattt gggtgtcgac aaggcttttg aggcaggcga aaaattcgga ctcaacatgg
     5101 aaaaggtcga ccgtgttctt ggtgtcgcct tgggaagcgg tgttgaaacc aaccctcttc
     5161 aaatggctca agcatacgcc gcctttgcaa atgaaggttt aatgcctgaa gctcatttta
     5221 ttagtagaat tgaaaatgct agtggacaag ttattgcgag tcataaaaat tcacaaaaac
     5281 gggtgattga taagtctgta gctgacaaga tgaccagtat gatgttgggg actttcacca
     5341 acggtaccgg tattagttca tcgcctgcag actatgtcat ggcagggaaa actggaacaa
     5401 ctgaagcagt tttcaatccg gagtacacaa gtgaccagtg ggtaattggt tatactccgg
     5461 atgtagtgat tagccactgg cttggctttc cgaccactga tgaaaaccac tatctagctg
     5521 gctctacttc aaacggtgca gctcatgtct ttagaaacat tgccaatacc attttacctt
     5581 atacgccagg aagtaccttt acggttgaaa atgcttataa gcaaaatgga attgcaccag
     5641 ccaatacaaa aagacaagta caaaccaatg ataatagcca gacagatgat aatttgtctg
     5701 atattcgagg gcgtgcgcaa agtctagtag atgaggctag ccgggctatc tcagatgcga
     5761 agattaagga aaaggctcaa acaatatggg attcgatagt caatctattt cgctaagatg
     5821 cttgtcaaag cctagctttc ttgttataat ggataagatg gaggcgttat ggcactaaaa
     5881 aaagcaagcc tagcttgtgc ggtttgtggt tcgagaaact attcaatcaa gatcagcgga
     5941 aacctcaagc ctacacgact agaagtaaat aaattttgta agcattgtgg caagtacact
     6001 acacacagag aaacgagata ggagagagcg atgcgtttta ttggagatat ttttagactt
     6061 cttaaagaca caacatggcc aactcgcaag gaaagctgga gagattttcg ttctatcatg
     6121 gaatacacag ctttctttgt agtaattatt tacatttttg accagttgat tgtttcaggt
     6181 ttgattcgat ttattaacat tttttagaag attagtggag ttaattacac tagaaatctt
     6241 ctatttatga aaggaaatat catggatagt tttgataaag ggtggtttgt tttacaaact
     6301 tattctggtt atgaaaataa ggtgaaagaa aatctattac aacgtgcaca aacctacaat
     6361 atgttggata atattctacg cgttgaaatt ccaacacaaa cagtgcaagt tgaaaaaaat
     6421 ggaaagagaa aagaagtaga agaaaatcgc tttccaggtt atgttcttgt agaaatggtc
     6481 atgacagatg aagcttggtt tgttgttcga aacacaccaa atgttacagg atttgtcgga
     6541 tcacacggga acagatcaaa accaactcca ttattggaac aagaaattcg cgacattttg
     6601 gtatctatgg gacaaactgt tcaagaattt gatttcgatg ttgagattgg tcaaactgta
     6661 cgtattattg atggtgcttt tgcagactac actggtaaga ttacagaaat tgataataac
     6721 aaagtgaaaa tgattatctc tatgtttggt aatgacacag ttgcagaagt aaatctaaac
     6781 caaatcgcag aattataacc ctgagagagg ctggagc
//
LOCUS       DAVKNL010000084         6214 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVKNL010000084 DAVKNL010000000
VERSION     DAVKNL010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6214
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.084"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            1..171
                     /locus_tag="VW411_001934"
                     /pseudo
     CDS             1..171
                     /locus_tag="VW411_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(326..649)
                     /gene="tnpB"
                     /locus_tag="VW411_001935"
     CDS             complement(326..649)
                     /gene="tnpB"
                     /locus_tag="VW411_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU9794593.1"
                     /translation="MTIHLSSLGEVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKVLYWDGQGFWLLYKRFENGRLIWLSTEKDVKALTPEQVDWLMKGFS
                     ITPKI"
     gene            complement(630..815)
                     /locus_tag="VW411_001936"
     CDS             complement(630..815)
                     /locus_tag="VW411_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794594.1"
                     /translation="MPQPIVPVEIPQSRRFDSKKRNDILLKIRIGKLEVSFFQSLNLE
                     MVEQLLDKVLLYDNSSI"
     gene            complement(891..1010)
                     /locus_tag="VW411_001937"
                     /pseudo
     CDS             complement(891..1010)
                     /locus_tag="VW411_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218559.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATPase"
     gene            complement(1088..1699)
                     /locus_tag="VW411_001938"
     CDS             complement(1088..1699)
                     /locus_tag="VW411_001938"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEU9794595.1"
                     /translation="MVRLNVKPTRMELNNLKERLTTAERGHKLLKDKRDELMRRFISL
                     IRENNQLRKEVESYLIDNLKSFAVAKSLKNSQMVEELFSIPSKEIELFVEKENIMSVT
                     VPRMHMNITSQNENSEYSYLSSNSEMDDVFATMNSLIYKLLRLAEVEKTCQLMADEIE
                     KTRRRVNGLEYSIIPNLSETIHYIELKLEEAERANLVRIMKVK"
     gene            complement(1722..3107)
                     /locus_tag="VW411_001939"
     CDS             complement(1722..3107)
                     /locus_tag="VW411_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEU9794596.1"
                     /translation="MSVIKEYRTASEVVGPLMIVEQVNNVSYNELVEIQLHNGEIRRG
                     QVLEIHEDKAMVQLFEGSSGINLEKSKIRFAGHALELAVSEDMVGRIFNGMGKPIDGG
                     PDLIPEKYLDIDGQAINPVSRDYPDEFIQTGISSIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDENFAVVFAAMGITFEEAEFFMEELRKTGAIDRSVLFMNLAND
                     PAIERIATPRIALTAAEYLAFEKDMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLVGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSHE
                     LYNQGYRPPINVLPSLSRLKDKGSGEGKTRGDHAPTMNQLFAAYAQGKKVEELAVVLG
                     ESALSDVDKLYVRFTKRFEEEYINQGFYKNRNIEDTLNLGWELLSILPRTELKRIKDD
                     LLDKYLPLVEV"
     gene            complement(3108..4883)
                     /locus_tag="VW411_001940"
     CDS             complement(3108..4883)
                     /locus_tag="VW411_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEU9794597.1"
                     /translation="MTQGKIIKVSGPLVIASGMQEANIQDICRVGKLGLIGEIIEMRR
                     DQASIQVYEETSGLGPGEPVVTTGEPLSVELGPGLISQMFDGIQRPLDRFKLATHNDF
                     LVRGVEVPSLDRDIKWHFDSTIAIGQKVSTGDILGTVKETEVVNHKIMVPYGVSGEVV
                     SIASGDFTIDEVVYEIKKLDGSFYKGTLMQKWPVRKARPVSKRLIPEEPLITGQRVID
                     AFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFANVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMQRTVLIANTSNMPVAAREASIYTGITMAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLEEMPGDEGYPAYLGSRIAEYYERAGRSQVLGLPEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYKDSVGTYIDGKE
                     KTDWNSKITRAMNYLQRESSLEEIVRLVGIDSLSDNERLTMEIAKQIREDYLQQNAFD
                     SVDTFTSFAKQEAMLSNILTFADQANHALELGSYFTEIMEGTVAVRDRMARSKYVSED
                     RLDEIKIISNEITHQIHLILETGGL"
     gene            complement(4946..5266)
                     /locus_tag="VW411_001941"
     CDS             complement(4946..5266)
                     /locus_tag="VW411_001941"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEU9794598.1"
                     /translation="MEANKYKIGIIGSRDIILPFSMIGFDIFPAYQEQEAINTLRKLA
                     QSDYGVIYITEDIASMILDTIRHYDSQVVPAIILLPTHKQGLNLGLKRIEDNVEKAVG
                     HNIL"
     gene            complement(5256..6203)
                     /locus_tag="VW411_001942"
     CDS             complement(5256..6203)
                     /locus_tag="VW411_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEU9794599.1"
                     /translation="MTEEKFQKIIQSKDTETLAFILESTPYHLSIDILEDPSQTEISL
                     MTKLVNDYRWAYAESPSDIIVTLFALRYVYHNIKVLLKSKAAIKKDFSKLLIPIGIFD
                     IESLKHLVSSLHSDTLPDFMVREVESIWNEYETFNNIRVLDVGADLAYFKHLKLLSNE
                     LDEVLSQVIVEMIDFYNIITVKRGLSQNKSHGDILQLLSDEGSISAKEFIYIVENQEI
                     FVWFNKINPSLDSIFSTYELKMQDATISSSELEFLCDLLLYKTLDQGRYNVEGPLVLA
                     RYLLGCEFEVKNLRMIISALQNTIPFESIKERIRPHYGS"
BASE COUNT         2014 a         1253 c          929 g         2018 t
ORIGIN      
        1 attgaacaag tgtggaaaga gattcgtaaa cgtggattta agaataaagc cttttaaact
       61 ttggaagatg tcatgaatca actccaagat gttatacaag gactggagaa ggaggtgata
      121 aagtccatcg ttaatcggag atggactaga atgctttttg aaagcagatg agtataaaaa
      181 gaaagtcctc atttcaatag aaatcacgac tttctgatgg atttatagta aaatgaaata
      241 agaacaggac aaatcgatca ggacagtaaa atcgatttct aacaatgttt tagaagcaga
      301 ggtgtactat tctagtttca atctactata tttttggagt gatagaaaag cccttcataa
      361 gccagtctac ttgttctggt gtgagagctt tgacatcctt ttctgtactt agccaaatca
      421 atctgccgtt ctcaaagcgt ttatatagta gccaaaatcc ttgaccatcc cagtaaagga
      481 ctttaaagcg gtctttacgt ccaccacaaa agagaaagac ttgaccggag aaaggatcca
      541 attcaaagtg ggttttaacc agataagcca gtgaatcgat tccttgtctc atatcagttt
      601 tcccacacac gagatagacc tcccctaggc tagatagatg aattgtcata gagcaacacc
      661 ttatccaaaa gctgttctac catttcgaga ttgagagatt gaaaaaaact tacttcaagc
      721 ttgccaatac gaattttaag cagaatatca tttctctttt tagaatcaaa acgacgagat
      781 tgtggaatct ctacaggaac aataggttgt ggcattaaaa tatcctccaa tagttttact
      841 ttactaatag tatacaggag gagctccatg aatgatagat accttgttat gacgcgctta
      901 ctttctatat aattacattt acgatttact aatcctaaat ctagattagt agaaacattt
      961 tcattatcta acaaaccaaa attttgaaaa agatatcctg atactgtcaa taaaaaaagc
     1021 ttagaaaggc ttaaagcctt atccaagctg atatttgttc atctaattaa taatctaaat
     1081 aaaggatcta cttcactttc ataatacgaa ctaaattggc tctttctgcc tcctctagtt
     1141 tcaattctat ataatgaata gtttccgaca agtttggaat aatcgagtat tctaaaccat
     1201 ttacacgtct acgtgttttt tctatttcat cagccattaa ctgacacgtt ttttcaactt
     1261 ctgccagtct tagtaattta taaattaaac tattcattgt agcaaataca tcatccattt
     1321 cactattaga agataaatag ctgtattcac tgttctcatt ttgagaagta atattcatat
     1381 gcattctagg aactgttaca ctcatgatat tttctttctc aacaaataat tcaatttctt
     1441 tcgatggaat tgaaaataat tcctccacca tttgagaatt ctttaatgat ttagcaactg
     1501 caaaggattt tagattatca attagataac tttccacttc tttccgaagt tgattattct
     1561 cacgaatcaa agaaataaat cgcctcatca attcatctct tttatccttt aataacttat
     1621 gtccacgttc agctgttgtc aaacgttcct ttaagttatt caattccata cgagttggtt
     1681 ttacattcaa acgtaccata gataatcact ccatttccgg attaaacttc taccaaaggt
     1741 aagtatttat caagcaaatc atctttgata cgttttaact ctgttctagg aagaattgat
     1801 agtaattccc acccaagatt caacgtatct tctatatttc gatttttata aaatccttgg
     1861 tttatgtact cttcttcaaa acgctttgta aacctcacat acaatttatc tacatcagat
     1921 aaagccgatt ctcctaatac tactgctaac tcttcaacct ttttcccttg ggcataggct
     1981 gcaaacagtt gattcatagt tggagcatga tctccacgag ttttaccttc tccagatccc
     2041 ttatctttta atcgagagag agaaggtaaa acattgattg gtggacgata accttgatta
     2101 tacaactcat gcgacaaaat aatttgccct tcagtaatgt atccagttaa atcaggaatt
     2161 ggatgtgtta tgtcatcttc tggcattgtt aaaataggaa tctgtgtcac cgaacctttt
     2221 ttaccaacta agcgaccagc cctttcgtat agagttgata aatttgtata taaatatccc
     2281 ggatagcctc gtctccctgg aacttcacgg cgagctgccg agacttcacg taacgcttca
     2341 caatagttag tcatatccgt catgataact agaacgtgca tatctttttc aaaagctaga
     2401 tactctgccg cagttaaagc aatgcgggga gttgcaatac gctcaattgc aggatcattt
     2461 gccaagttca taaataaaac cgaacgatcg atcgctcctg tttttctgag ttcttccata
     2521 aaaaactcag cttcttcaaa agtaataccc attgctgcaa atacaaccgc aaaattttca
     2581 tcagaattta aaacagtcgc ttgtcttgct atctgagcag ctaattcatt atgaggtaag
     2641 cccgaacctg aaaatactgg taatttttga ccacgtacaa gagtattcaa atgatcaata
     2701 gaggagatcc ctgtctgaat aaattcatct ggataatctc tagatacagg attaatagct
     2761 tgaccatcaa tatctaaata tttctctgga attaaatctg gtccaccatc aattggtttt
     2821 cccatcccat taaaaatacg accaaccata tcctcagata cagccaattc taatgcatga
     2881 ccagcaaaac gaattttaga cttttctaaa tttattccac tagatccttc aaaaagctga
     2941 accattgctt tatcttcgtg gatctctaaa acttgtccac gacgaatttc tccattatga
     3001 agttgaattt caactaactc attgtaagac acattattta cttgttcaac aatcataaga
     3061 ggcccaacaa cttcactagc agttctgtat tcttttataa cactcattta tagacctcct
     3121 gtttctaata tcaaatgaat ttgatgtgta atctcatttg atataatttt gatttcatct
     3181 aatctatctt ctgaaacata tttacttctc gccatacggt ctcgaactgc cacggtacct
     3241 tccataatct ctgtaaagta agaacccaac tctaaagcat gatttgcctg atcagcaaaa
     3301 gtgagaatat tacttagcat tgcttcttgt tttgcaaacg aagtgaatgt atctaccgaa
     3361 tcaaaagcgt tctgttgcaa ataatcttct cgaatttgtt tagcaatttc catcgttagt
     3421 cgttcattat cagacagaga atcaattcca acaagacgaa caatttcctc taaactagat
     3481 tcccgttgta agtagttcat cgcacgagtt attttactat tccaatctgt cttctcttta
     3541 ccatctatat aagtgcccac actgtcttta tatagtgaat aagatgtaag ccagttaatt
     3601 gcaggaaaat gacgtcgctg tgccaacgga gcatcaagcc cccaaaaaac tttcacaatc
     3661 cgtaaagtgt tttgagtaac tggttctgaa atatctccac caggtggcga tacagctcca
     3721 atagcagtaa tcgttccttc acgttctgga agccctagaa cctgagaacg tcctgctctt
     3781 tcataatatt cagcgatacg acttcccaga taagcaggat aaccctcatc accaggcatt
     3841 tcttctagac gtcctgacat ttcacgtagc gcttctgccc aacgtgaagt tgaatcagcc
     3901 ataatggcga cagagtagcc catatcacga aaatactcag ccatggtaat tcctgtataa
     3961 attgaagcct cacgagcagc aacaggcata tttgaagtat tagcaatcag aactgtccgt
     4021 tgcataattg attgtccggt attagggtca atcaactcag gaaactcatt cagtacatcc
     4081 gtcatttcat ttccacgttc tccacaaccg acataaataa caatatcaac attggcaaat
     4141 ttagctactt ggtgttgtac aactgtcttt cctgctccaa acggtccagg aactgctgca
     4201 gctccccctt tggttactgg aaagaatgca tcaataactc gttgacctgt gattaatggt
     4261 tcttctggaa ttaaacgttt agaaacagga cgcgccttgc ggacaggcca tttttgcata
     4321 agcgttcctt tatagaaact accgtccaat ttttttattt catatacaac ttcatcaatt
     4381 gtaaaatcgc cagatgcaat agaaacgact tctccagata ctccataagg aaccataatt
     4441 ttatgattaa ctacctcggt ttccttgaca gttccaagaa tatcacccgt actcactttt
     4501 tgaccaattg ctatagtgga atcaaaatgc cacttaatat ctctatccaa acttggaact
     4561 tctaccccac gaactagaaa atcattatga gtagccaatt taaatcgatc taatgggcgt
     4621 tgtatgccat caaacatttg agaaatcaat cctggcccta attcaaccga gagaggttct
     4681 ccagttgtaa caacaggttc tcccggacca agaccagatg tttcttcata gacttggata
     4741 gatgcctgat ctcttctcat ttcaataatt tcaccgatta accctagctt acctacacgg
     4801 caaatatctt gaatattagc ctcctgcata cctgatgcaa taactagagg tcccgatact
     4861 tttataatct tcccttgagt caaagttatt ctccttacta agtccaaaaa ttatagaata
     4921 atattacaga caattttgta cattattata aaatattgtg tcctactgct ttctctacat
     4981 tatcctctat acgttttaat cctaaattta aaccttgttt atgagtcggt aataaaataa
     5041 tagcaggcac aacttgggaa tcataatggc gaattgtatc taatatcatt gaagcaatgt
     5101 cttcagtgat ataaatgaca ccataatcag attgagctaa ttttcttagt gtatttatag
     5161 cttcttgttc ttggtaggca ggaaatatat caaacccaat catgctaaat ggtaaaataa
     5221 tatcacggct accaattatg ccaattttat acttattagc ttccataatg tgggcgtatc
     5281 ctttctttta ttgattcaaa gggaattgta ttttgaagag ctgatatgat cattctgaga
     5341 ttctttactt caaactcaca tcccaataaa tatctagcaa gaactaacgg cccctctaca
     5401 ttgtaccttc cttgatctaa agttttatac aatagtaaat cacataaaaa ttctaactca
     5461 gaagatgaaa ttgttgcgtc ctgcatcttc aattcataag ttgaaaagat tgaatctaag
     5521 cttggattta ttttattgaa ccacacaaat atttcttgat tttctacaat gtatataaat
     5581 tctttagcag aaatacttcc ttcatctgaa agtaattgta aaatatcccc atgactctta
     5641 ttttgagata aaccacgttt tacagtaata atattataaa agtcaatcat ttcgacaata
     5701 acctgagaca gtacctcatc taactcatta gataaaagtt tcagatgttt aaaatatgct
     5761 agatcagctc cgacatcaag tacacgaata ttattaaaag tttcatactc attccaaatt
     5821 gattctactt cacgaaccat aaaatcagga agtgtatctg aatgtaagga agaaactaaa
     5881 tgttttaaac tttctatatc aaaaatccct attggaatta ataatttaga aaaatctttc
     5941 ttaattgccg ccttagattt taataaaact ttgatattat gataaacata tcgtaaagca
     6001 aataaagtca caattatatc agacggactt tcagcatagg cccatctata atcattgact
     6061 aattttgtca ttagcgaaat ctctgtctga ctaggatctt ctaagatgtc aatcgataaa
     6121 tgatagggag ttgattctaa gataaatgcc aatgtctccg tatctttgga ttgtataatt
     6181 ttttgaaatt tttcttctgt aataaaatcg tttt
//
LOCUS       DAVKNL010000085         6026 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVKNL010000085 DAVKNL010000000
VERSION     DAVKNL010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.085"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            224..1669
                     /gene="cps4A"
                     /locus_tag="VW411_001943"
     CDS             224..1669
                     /gene="cps4A"
                     /locus_tag="VW411_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4A"
                     /protein_id="HEU9794600.1"
                     /translation="MSRRFKKSRSQKVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSEIENVTQLTSVTAPTGTNNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKSQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKLIDLLGGIDVYNDQ
                     EFTAHTNGKYYPAGNVHLDSEQALGFVRERYSLADGDRDRGRHQQKVIVAILQKLTST
                     EVLKNYSTIINSLQDSIQTNMPLETMINLVNAQLESGGNYKVNSQDLKGTGRMDLPSY
                     EMPDSNLYVMEIDDSSLAVVKAAIQDVMEGR"
     gene            1671..2402
                     /gene="cps4B"
                     /locus_tag="VW411_001944"
     CDS             1671..2402
                     /gene="cps4B"
                     /locus_tag="VW411_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4B"
                     /protein_id="HEU9794601.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVASDLVIAYGAEIYYTPDVLDKLEKKRIPTLNDS
                     RYALIEFSMNTPYRDIHSALSKILMLGITPVIAHIERYDALENNEKRVRELIDMGCYT
                     QVNSSHVLKPKLFGERYKFMKKRAQYFLEQDLVHVIASDMHNLDGRPPHMAEAYDLVT
                     QKYGEAKAQELFIDNPRKIVMDQLI"
     gene            2411..3103
                     /gene="cpsC"
                     /locus_tag="VW411_001945"
     CDS             2411..3103
                     /gene="cpsC"
                     /locus_tag="VW411_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HEU9794602.1"
                     /translation="MKEQNTIEIDVFQLVKSLWKRKLMILIVALVTGAGAFAYSTFIV
                     KPEYTSTTRIYVVNRNQGDKPGLTNQDLQAGTYLVKDYREIILSQDVLEEVVSDLKLD
                     LTPKGLANKIKVTVPVDTRIVSISVNDRVPEEASRIANSLREVAAQKIISITRVSDVT
                     TLEEARPAISPSSPNIKRNTLIGFLAGVIGTSVIVLHLELLDTRVKRPEDIENTLQMT
                     LLGVVPNLGKLK"
     gene            3113..3796
                     /gene="cps4D"
                     /locus_tag="VW411_001946"
     CDS             3113..3796
                     /gene="cps4D"
                     /locus_tag="VW411_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4D"
                     /protein_id="HEU9794603.1"
                     /translation="MPTLEIAQKKLEFIKKAEEYYNALCTNIQLSGDKLKVISVTSVN
                     PGEGKTTTSINIAWSFARAGYKTLLIDGDTRNSVMLGVFKSREKITGLTEFLSGTADL
                     SHGLCDTNIENLFVVQSGSVSPNPTALLQSKNFNDMIETLRKYFDYIIIDTPPIGIVI
                     DAAIITQKCDASILVTATGEANKRDIQKAKQQLKQTGKLFLGVVLNKLDISVNKYGVY
                     GSYGNYGKK"
     gene            4137..4772
                     /gene="cps4E"
                     /locus_tag="VW411_001947"
     CDS             4137..4772
                     /gene="cps4E"
                     /locus_tag="VW411_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4E"
                     /protein_id="HEU9794604.1"
                     /translation="MKNGNRIYSWRLFMYGIIKRLGDILLSLIGIIILCPVFMIIAIA
                     IKLDSEGPVIFKQKRFGIHKEYFYILKFRSMKIDAPKNVAPRNLYNPEQWITKVGAFL
                     RKTSLDELPQLFNILVGNMSIVGPRPAGINELDLIAERDKYGANDILPGLTGWAQING
                     RDTLSVEMKTELDGYYVKHLSLIMDIRCIVKTIPYVLKRKGIVEGSGKKES"
     gene            4781..6010
                     /gene="cps4F"
                     /locus_tag="VW411_001948"
     CDS             4781..6010
                     /gene="cps4F"
                     /locus_tag="VW411_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4F"
                     /protein_id="HEU9794605.1"
                     /translation="MKILFVCQHYKPEPFRLSDICEDLVRKGHEVSVLAGIPNYPEGK
                     IYADYRHNKKRREIIEGVTIYRSYTIPRKKSVVFRLLNYFSFAISSTLGVLLGRYKTK
                     DGSNFDCVFVNQLSPVMMAWAGMAYKKKYKKPMFLYCMDVWPDSLTVGGVKQDGLIFK
                     LFKFISKKVYRASDYIFVTSPSFKNYFVKQFDISEQKITYLPQYAEDLFIPDESIVNK
                     ESVDLTFAGNIGKAQNLETILKAASLIEKNTNLPKKIHFHFVGDGTELLSMKALAHEL
                     ELKNISFYGRRSLEEMPSFYKKSDAMLVSLIGDSIVSRTIPGKVQSYMAAGKPIIGAI
                     SGDAKIIVEEANCGYVSPERDVKQLAKNICKFSMLSIKRQRELGKKARCYYENHFSKE
                     QFMLELETCLERESKKE"
BASE COUNT         1988 a          906 c         1267 g         1865 t
ORIGIN      
        1 ttagattgaa actagaatag tacacctctg cttctaaaac attgttagaa atcgatttga
       61 ctgtcctgaa cgatttgttc tgttcttatt tcattttact atatttttgt ttcgcgggaa
      121 gtctactaag atacttaaag atgcagatag taaaaataaa ggtgtagaca ttaccgtaaa
      181 aaagtgatat aatcgtataa tgttcaatgt ataggtatta atcatgagta gacgttttaa
      241 aaaatcacgt tcacagaaag tgaagcgaag tgttaatata gttttgctga ctatttattt
      301 attgttagtt tgttttttat tgttcttaat ctttaagtac aatatccttg cttttagata
      361 tcttaatcta gtggtaactg cgttagtcct actagttgcc ttggtagggc tactcttgat
      421 tatctataaa aaagctgaaa agtttactat ttttctgttg gtgttctcta tccttgtcag
      481 ctctgtgtcg ctctttgcag tacagcagtt tgttggactg accaatcgtt taaatgcgac
      541 ttctaattac tcagaatatt caatcagtgt cgctgtttta gcagatagtg agatcgaaaa
      601 tgttacgcaa ctgacgagtg tgacagcacc gactgggact aataatgaaa atattcagaa
      661 attactagct gatatcaagt caagtcagaa taccgatttg acggtcaacc agagttcgtc
      721 ttacttggca gcttacaaga gtttgattgc aggggagact aaggccattg tcctaaatag
      781 tgtctttgaa aacatcatcg agtcagagta tccagactac gcatcgaaga taaaaaagat
      841 ttatactaag ggattcacta aaaaagtaga agctcctaag acgtctaaga gtcagtcttt
      901 caatatctat gttagtggaa ttgacaccta tggtcctatt agttcggtgt cgcgatcaga
      961 tgtcaacatc ctgatgactg tcaatcgaga taccaagaaa atcctcttga ccacaacgcc
     1021 acgtgatgcc tatgtaccaa tcgcagatgg tggaaataat caaaaagata aattgactca
     1081 tgcgggcatt tatggagttg attcgtccat tcacacctta gaaaatctct atggagtgga
     1141 tatcaattac tatgtgcgat tgaacttcac ttcgtttttg aaattgattg atttgttggg
     1201 tggaattgat gtttataatg atcaagaatt tactgcccat acgaatggaa agtattaccc
     1261 tgcaggcaat gttcatcttg attcagaaca ggctctcggt tttgttcgtg agcgctactc
     1321 cctagcagat ggcgatcgtg accgcgggcg ccatcaacaa aaggtgattg tggctatcct
     1381 tcaaaaatta acgtcaaccg aagtgctgaa aaattatagt acgatcatta atagcttgca
     1441 agattctatc caaacaaata tgccacttga gaccatgata aatttggtca atgctcagtt
     1501 agaaagtgga gggaattata aagtaaattc tcaagattta aaagggacag gtcggatgga
     1561 tcttccttct tatgaaatgc cagacagtaa cctctatgtg atggaaatag atgatagtag
     1621 tttagctgta gttaaagcag ctatacagga tgtgatggag ggtagatgaa atgatagaca
     1681 tccattcgca tatcgttttt gatgtagatg acggtcccaa gtcaagagag gaaagcaagg
     1741 ctctcttggc agaatcctac agacaggggg tgcgaaccat tgtttctacc tctcaccgtc
     1801 gcaagggcat gtttgaaact ccggaagaga agatagcaga aaactttctt caggttcggg
     1861 aaatagctaa ggaagtggcg agtgacttgg tcattgctta cggggctgaa atttattaca
     1921 caccagatgt tctggataag ctggaaaaaa agcggattcc gaccctcaat gatagtcgtt
     1981 atgccttgat agagtttagt atgaacactc cttatcgcga tattcatagc gccttgagca
     2041 agatcttgat gttgggaatt actccagtca ttgcccacat tgagcgctat gatgctcttg
     2101 aaaataatga aaaacgcgtt cgagaactga tcgatatggg ctgttacacg caagtaaata
     2161 gttcacatgt cctcaaaccc aaactttttg gcgaacgtta taaattcatg aaaaaaagag
     2221 ctcagtattt tttagagcag gatttggttc atgtcattgc aagtgatatg cacaatctag
     2281 acggtagacc tcctcatatg gcagaagcat atgaccttgt tacccaaaaa tacggagaag
     2341 cgaaggctca ggaacttttt atagacaatc ctcgaaaaat tgtaatggat caactaattt
     2401 aggagaaatg atgaaagaac aaaatacgat agaaatcgat gtatttcaat tagttaaaag
     2461 cttgtggaaa cgcaagctaa tgattttaat agtggcactt gtgacaggtg cgggggcttt
     2521 tgcatatagc acttttattg ttaagccaga atatacgagt accacgcgaa tttacgtagt
     2581 gaatcgcaat caaggagaca agccggggtt gacaaatcag gatttgcagg caggaactta
     2641 tctggtaaaa gactaccgtg agattatcct ttcgcaggat gttttggagg aagttgtttc
     2701 tgatttgaaa ctagatttga cgccaaaagg tttggctaat aaaattaaag tgacagtacc
     2761 agttgatacc cgtattgtct ctatttcagt taatgatcga gttcctgaag aggcaagccg
     2821 tatcgctaac tctttgagag aagtagctgc tcaaaaaatt atcagtatta ctcgtgtttc
     2881 tgacgtgaca acactggagg aggcaaggcc ggcgatatcc ccgtcttcgc caaatattaa
     2941 acgcaataca ctaattggtt ttttggcagg ggtgattgga actagtgtta tagttcttca
     3001 tcttgaactt ttggatactc gtgtgaaacg tccggaagat atcgaaaata cattgcagat
     3061 gacacttttg ggagttgtgc caaacttggg taagttgaaa taggagagaa ggatgccgac
     3121 attagaaata gcacaaaaaa aactggagtt cattaagaag gcagaagaat attacaatgc
     3181 cttgtgtaca aatatacagt tgagcggaga taaactaaaa gtaatttccg ttacttctgt
     3241 taaccctggg gaaggaaaaa caactacttc cataaatata gcatggtcgt ttgcgcgtgc
     3301 aggctataaa actcttttga tcgatggcga tactcgaaat tcagttatgt taggagtttt
     3361 taaatctcgt gaaaaaatta cagggctaac agaattttta tctgggacag ctgatttatc
     3421 tcacggttta tgtgatacaa atattgaaaa tttatttgta gttcaatcgg gatctgtatc
     3481 accaaaccct acagccttgt tacaaagtaa aaattttaat gatatgattg aaacattgcg
     3541 taaatatttt gattatatca ttattgatac accgcctatt ggaattgtta ttgatgcggc
     3601 aattatcact caaaagtgtg atgcgtccat cttggtaaca gcaacaggtg aggcgaataa
     3661 acgtgatatc caaaaagcga aacaacaatt aaaacaaaca gggaaactgt tcctaggagt
     3721 tgttttaaat aaattggata tctcggttaa taagtatgga gtttacggtt cctatggaaa
     3781 ttatggtaaa aaataaaatg aatatagtat ggaaggggac tacaatataa ctctggcatt
     3841 cttacagagc atacttatca gtttattagc ttatgtactg attgcaattt cagaaaccga
     3901 tatgtcttca aataatattc ggtctatttg gaagaataat tgaactggac cttctaattc
     3961 attattgaga actcatatat tactgaaaca aataaatttt gtttagatgg agaaaagttt
     4021 tatctgacgt agaggataaa agaattatat ttctgaaaat cattgagtga ccaaatatat
     4081 attgagtttc tgtagttgca tgtactgcct gatttaattt gtcaaatgga aatagaatga
     4141 aaaatggaaa tagaatttat agttggaggt tgtttatgta cggtataata aaacgattag
     4201 gtgatatatt attatcttta atagggataa taatattgtg tccggttttt atgataattg
     4261 caattgcgat taaacttgat tcagaaggtc cggttatatt taagcaaaaa cgctttggta
     4321 ttcataaaga atacttctat attttgaaat ttaggtctat gaaaatagat gcacctaaaa
     4381 atgtggcgcc tcgaaactta tataatccag agcaatggat tacaaaagta ggggctttct
     4441 tgcgaaaaac atctttggat gaactaccac aattgtttaa tattcttgtt ggtaatatga
     4501 gtattgtagg tcctagacca gcgggtataa atgaactaga tttgattgca gagagagata
     4561 agtatggagc aaatgatatc ttgccagggt taactggatg ggcacaaatt aacgggcgtg
     4621 atactttgtc tgttgagatg aagacggagt tagatggcta ctatgttaaa catctgtctt
     4681 tgataatgga tattagatgt atagttaaga caatacctta cgtactgaaa cgaaaaggta
     4741 ttgtagaggg tagtggtaag aaagaaagtt aaattggaca atgaaaatac tatttgtttg
     4801 ccaacattat aagccagaac cattcaggtt gtcagatatt tgtgaagatt tagttcgaaa
     4861 agggcatgaa gtctctgttt tggctgggat tcctaattac cctgaaggga agatatatgc
     4921 agattatcgt cataataaaa aaagacgtga gattatagaa ggtgttacga tatatcgttc
     4981 ttatacaatc cctagaaaaa aaagtgttgt atttcgattg ttgaattatt ttagctttgc
     5041 aattagttct actttaggag ttttattggg gaggtataaa acgaaagatg gatcgaattt
     5101 tgactgtgta ttcgttaacc aattgtctcc agttatgatg gcatgggctg gtatggctta
     5161 taaaaaaaaa tataagaaac cgatgtttct atattgtatg gatgtttggc cagatagttt
     5221 aaccgtaggt ggagtgaaac aagatggctt gattttcaag ctgtttaaat ttatctcaaa
     5281 aaaagtttac cgagctagtg attatatatt tgtcactagt ccatcattta aaaattattt
     5341 tgtgaagcaa tttgacatat ccgaacaaaa gattacatat ttgccacaat atgcagaaga
     5401 tctttttatc cctgatgaat ctatagttaa taaagaaagt gttgacctaa cttttgctgg
     5461 taatattggc aaagcacaaa atttggaaac tattttgaaa gctgccagtt tgatagagaa
     5521 gaataccaat ttacccaaga aaattcattt tcattttgtt ggagatggta cggaattgtt
     5581 aagcatgaaa gcattagctc atgaattgga gttaaagaat atttccttct atggaagacg
     5641 ttctttggag gaaatgccat ccttctataa aaaatcagat gctatgttag tttctttaat
     5701 aggagactcg atagtttctc gtactatacc tgggaaggta caatcttata tggcggcagg
     5761 caaaccaatt ataggtgcaa tttcaggaga tgctaaaata attgtagaag aagcaaattg
     5821 tggatatgtt agtcccgaac gagatgtaaa acaattggca aaaaatattt gtaaatttag
     5881 tatgttatct attaagagac aaagagagtt aggaaagaaa gctcgttgtt actatgaaaa
     5941 tcacttttca aaagagcagt ttatgctcga actggagaca tgtttagaga gggaaagtaa
     6001 gaaagaataa tttaatgaga gtactg
//
LOCUS       DAVKNL010000086         5884 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVKNL010000086 DAVKNL010000000
VERSION     DAVKNL010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5884
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.086"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            1..173
                     /locus_tag="VW411_001949"
                     /pseudo
     CDS             1..173
                     /locus_tag="VW411_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            340..540
                     /locus_tag="VW411_001950"
     CDS             340..540
                     /locus_tag="VW411_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794606.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            582..986
                     /locus_tag="VW411_001951"
     CDS             582..986
                     /locus_tag="VW411_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794607.1"
                     /translation="MDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETNSVPA
                     GVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKAQNLL
                     QETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            1217..1924
                     /gene="ccdA2"
                     /locus_tag="VW411_001952"
     CDS             1217..1924
                     /gene="ccdA2"
                     /locus_tag="VW411_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU9794608.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYGMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            1935..2492
                     /gene="sdbB"
                     /locus_tag="VW411_001953"
     CDS             1935..2492
                     /gene="sdbB"
                     /locus_tag="VW411_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEU9794609.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            2866..4257
                     /locus_tag="VW411_001954"
     CDS             2866..4257
                     /locus_tag="VW411_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU9794610.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4400..5317
                     /gene="adcAII"
                     /locus_tag="VW411_001955"
     CDS             4400..5317
                     /gene="adcAII"
                     /locus_tag="VW411_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEU9794611.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            5325..>5884
                     /locus_tag="VW411_001956"
     CDS             5325..>5884
                     /locus_tag="VW411_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU9794612.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQEH
                     SHNHGGGSNDQAVVAARAQGRYTTDDG"
BASE COUNT         1796 a         1045 c         1266 g         1777 t
ORIGIN      
        1 gaggcaaagt atctggaaga agatatcaga ggatttcttt ggttgcaggt ctaacaaatg
       61 gtgaattaat cgctccaatg acttacgaag agacgatgac gagcgacttt tttgaagctt
      121 ggtttcagaa gtttctctta ccaacattaa ccacaccatc ggttattata gtaaaatgaa
      181 ataagaatag gacaaatcga tcaggacagt aaaatcgaat tctaacaatg ttttagaagt
      241 ccatatgtac tattctagtt tcaatatacc ataactgttc ctagtcaacc tgcaagccct
      301 gtcccagctc caagctcagc tccgactcaa gatcctagag tggtagcaag cgcttcggct
      361 agctcaacga gcacacaagc tcaagagcaa gttgacaagt ctgaacttcg tgccttgagt
      421 caagagttag accaacgctt gaaagctttg gcaacagtat ctgatccgaa aatcgatgca
      481 accaaggctg tcctcctaga tgctcaaaaa gctccggaag atagtgcctt gacagagtaa
      541 ggtctgcata ctgcagtaga gtctgtgaag gctgggctgc cttggactct ctcaaagatg
      601 tgaaagcaaa tgctagcgac agcaagcctg cacaggacaa gaaggatgca aaacaaggaa
      661 cggaagatag taaggattca gataagatga ctgaaacaaa ctcagttccg gcaggagtga
      721 ttgtggtcag tctacttgcc ctcctaggcg tgattgcctt ctggctgatt cgccgtaaga
      781 aagagtcaga aatccagcaa ttaagcacgg aattgatcaa ggttctagga cagctagatg
      841 cagaaaaagc ggataaaaaa gtccttgcca aagcccaaaa ccttctccaa gaaacccttg
      901 atttcgtgaa agaagaaaat ggctcagcag agacagaaac taaactagta gaggagctta
      961 aagcaatcct tgacaaactc aagtaaaaca tgatttatga aatgtcagac ctaaaagaca
     1021 ttttcgttca caaaagcagc gatgagtaat ccttgtcgtt gctttttggc tattttgggg
     1081 gataagatcc gccttgacta gcaaaagaaa agattggtcc aatagtgccc gatacgattt
     1141 tgcgtagcag aaagcttaca aataaaattg aatatagtaa aataggatta gaattattaa
     1201 gaaagttggt tctttattgg aaacgatagt atttttaatc tctgtttttc tagcaggtgt
     1261 tttatccttt ttttctcctt gtatttttcc tcttctgcca gtctatgctg ggattttatt
     1321 ggatgatcaa gaaagtgcaa aaagcttttc tttgtttggg agaaaggttc tctggtcagg
     1381 cttgattcga acactttgct ttatcgctgg tatctctctc attttcttta ttctaggctt
     1441 tggtgctggt tactttggtc atattctcta tgcaaattgg tttcgatatg gcatgggagc
     1501 tattattatc attttgggtc ttcaccagat ggaaattttt catttgaaga aattagaagt
     1561 tcaaaaaagt tttaccttta aaaaatcaga ttctaatcgt tattggtcag cttttttact
     1621 tggtattacc tttagctttg gttggacacc ttgtattggt ccagttttaa gttctgtttt
     1681 agcacttgcg gcttctggag gcaatggcgc ttggcaagga gcgatttata ctctcattta
     1741 cactctgggc atggcccttc ctttcttggt attggcacta gcttcaggtc tagtcatgcc
     1801 atattttagt aaaatcaagc gtcatatgat gctactaaag aaaattggtg gtttcctcat
     1861 tgttttaatg ggaattttgt tactattagg acaagtaaat gttctagctg gaatttttga
     1921 ataaaggagg acaaatgaaa aaagtaatgt ttgctggctt aagtctcttg tcattagttg
     1981 tattgatggc ctgtggtgag gaagaaacta aaaagactca agcagcacaa cagccaaaac
     2041 aacaaacgac tgtacaacaa attgctgttg gaaaagatgc tccagacttc acattgcaat
     2101 ccatggatgg caaagaagtt aagttatctg attttaaggg taaaaaggtt tacttgaagt
     2161 tttgggcttc atggtgtggt ccatgcaaga aaagtatgcc agagttgatg gaactagcgg
     2221 cgaaaccaga tcgtgatttc gaaattctta ctgtcattgc accaggaatt caaggtgaaa
     2281 aaactgttga gcaattccca caatggttcc aggaacaagg atataaggat atcccagttc
     2341 tttatgatac caaagcaacc accttccaag cttatcaaat tcgaagcatt cctacagaat
     2401 atttaattga tagccaagga aagattggaa agattcaatt tggtgctatc agtaatgcgg
     2461 atgcagaagc agcatttaaa gaaatgaact agaatcgata aaatctgatt acaagatgta
     2521 gtcagatttt ggtaacttct aaaactaact tccagtttcc cacaacctag cttttagtat
     2581 gagaaaaacg cgtgacatca gtggaaatcc gtcttagact aacttccact ggttttcttt
     2641 ttcttgatat ataagggttc aaaagcgaaa agactcagaa aaagtgcacg acaaatagcc
     2701 ctaaaacaga gtcgtcttag agtaacttcc agttttggga gagagatgga agttactttg
     2761 agaagttacg gtagtcagat ttttttagaa aaacatttta taaatattca catatctcct
     2821 atatttatgg taaaatagaa ttatcagttt attttggagt caaagatgaa tatatttaga
     2881 acaaagaatg ttagtttaga taaaacagag atgcataggc atttgaagtt atgggatttg
     2941 attttgctgg gtatcggagc catggtaggg acaggcgtct ttacaatcac aggtactgca
     3001 gctgcaacac ttgctggccc agccctagtg atttcaatcg ttatttctgc cttgtgtgtg
     3061 ggattatcag ccctcttttt tgcagaattt gcctcgcgag tacccgctac aggaggtgcc
     3121 tatagttacc tctatgctat cttaggagaa ttccctgcct ggttggctgg ttggttaacc
     3181 atgatggagt tcatgacagc catatcaggc gtagcttcgg gttgggcagc ttattttaaa
     3241 ggcttgctct ctcaatacgg gatagccttt cctcaggctt taaatggtac ctttaatcct
     3301 caagcaggga catttgttga tttattgcct attcttgtct tggtcttggt gacttcgctt
     3361 gttttactaa atgccaaggc agccttacgc tttaattcga ttctagttat tttgaaattt
     3421 tccgctttag ctctctttgt tttagtagga atttggaata ttaagtttga taattggagc
     3481 aattttgctc cttacggttt tggacaaatc tatggagcta gcactggtat tatggctggt
     3541 gcgtccttga tgttcttcgg ttttttggga tttgaatcca tctctatggc tgtagatgaa
     3601 gtcaagactc ctcaaaaaaa tattcctaga gggattgtct tatcgctttc tatcgtaacc
     3661 attctctatg ccttggtgac ccttgttttg actggtgtgg ttcactatag tcatttaaat
     3721 gtcgatgatg ccgttgcctt tgcccttcgt agtgttggga ttagttgggc agccaactat
     3781 gtgtcattag tggctatctt gaccttgatt acagtttgta tttcgatgac ctatgcccta
     3841 tcgcgtatga tttacagttt agcacgtgac ggcttagtgc ctgctgcctt taaggaactg
     3901 acgaagacta gcaagatacc aaagaatgct actatcttga caggtctagc ttcagcagta
     3961 gcagcaggaa tgttcccact agccagtatt gcagcctttt taaatatttg taccttagcc
     4021 tacttgatca tgttggctta tggtctgatt cgcttacgga aagaaaaagg aatgcccaaa
     4081 gctggggaat ttaaaacacc attggtacct ctgttaccaa ttttatcgat catcatttgt
     4141 ttatccttca tgttacagta taatatgaat acctggattg ctttcctagt tgcgttgtta
     4201 gtaggaagta ttatttactt cacttatggc tataagcatt ctaccataga agaataaagt
     4261 gaaagtctat tgagttgttg acactcagta gatttttata tgtgaaagaa atttcaaatt
     4321 ttttctaaaa atatcttgac agattaaatt ttcaggagta gaatatttac tagttaatta
     4381 aatggttaag gagttgttca tgaagaaaca aaatttattt ttagtcctgt taagtgtctt
     4441 tcttttgtgc ttgggggctt gtggtcaaaa ggaaagtcag acaggaaagg ggatgaaaat
     4501 tgtgaccagt ttttatccta tctacgctat ggttaaggaa gtatctggtg acttgaatga
     4561 tgttcggatg attcagtcaa gtagtggtat tcactccttt gaaccttcgg caaatgatat
     4621 cgcagccatc tatgatgcag atgtctttgt ttaccattct catacactcg aatcttgggc
     4681 aggaagtctg gatccaaatc taaaaaaatc caaagtgaag gtcttagagg cttctgaggg
     4741 aatgaccttg gaacgtgtcc ctggactaga ggatgtggaa gcaggggatg gagttgatga
     4801 aaaaacgctc tatgaccctc acacatggct agatcctgaa aaagctggag aagaagccca
     4861 aattatcgct gataaacttt cagaggtgga tagtgagcat aaagagactt atcaaaaaaa
     4921 tgcgcaagcc tttatcaaaa aagctcagga attgactaag aaattccaac caaaatttga
     4981 aaaagcgact cagaaaacat ttgtaacaca acatacagcc ttttcttatc tagcgaagag
     5041 atttgggctt aatcaacttg gtattgcagg tatctctcct gaacaagaac caagtccacg
     5101 acaactaaca gaaattcagg aatttgttaa gacctataag gttaaaacga tttttacaga
     5161 aagtaacgct tcttcaaaag tagctgaaac tcttgtcaaa tcaacaggtg tgggtcttaa
     5221 aactctgaat cctttagagt cagacccaca aaatgacaag acctatttag aaaatcttga
     5281 agaaaatatg agtattctag cagaagaatt aaagtgagga aagaatgaaa attaataaaa
     5341 aatatctagc aggttcagtg gcagtccttg ccctaagtgt ttgttcctat gaacttggtc
     5401 gtcaccaagc tggtcaggtt aagaaagagt ctaatcgagt ttcttatata gatggtgatc
     5461 aggctggtca aaaggcagaa aacttgacac cagatgaagt cagtaagagg gaggggatca
     5521 acgccgaaca aatcgtcatc aagattacgg atcaaggtta tgtgacctct catggagacc
     5581 attatcatta ctataatggc aaggtccctt atgatgccat catcagtgaa gagctcctca
     5641 tgaaagatcc gaattatcag ttgaaggatt cagacattgt caatgaaatc aagggtggtt
     5701 atgttatcaa ggtagatgga aaatactatg tttaccttaa ggatgcagct catgcggata
     5761 atattcggac aaaagaagag attaaacgtc agaagcagga acacagtcat aatcacgggg
     5821 gtggttctaa cgatcaagca gtagttgcag ccagagccca aggacgctat acaacggatg
     5881 atgg
//
LOCUS       DAVKNL010000087         5722 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVKNL010000087 DAVKNL010000000
VERSION     DAVKNL010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5722
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.087"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            186..350
                     /locus_tag="VW411_001957"
     CDS             186..350
                     /locus_tag="VW411_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU9794613.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPAIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            434..1000
                     /gene="amaP"
                     /locus_tag="VW411_001958"
     CDS             434..1000
                     /gene="amaP"
                     /locus_tag="VW411_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEU9794614.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            1012..1182
                     /locus_tag="VW411_001959"
     CDS             1012..1182
                     /locus_tag="VW411_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEU9794615.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            1221..1829
                     /locus_tag="VW411_001960"
     CDS             1221..1829
                     /locus_tag="VW411_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9794616.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            1860..2063
                     /locus_tag="VW411_001961"
     CDS             1860..2063
                     /locus_tag="VW411_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU9794617.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(2173..2445)
                     /locus_tag="VW411_001962"
                     /pseudo
     CDS             complement(2173..2445)
                     /locus_tag="VW411_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547411.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2590..3036)
                     /locus_tag="VW411_001963"
     CDS             complement(2590..3036)
                     /locus_tag="VW411_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9794618.1"
                     /translation="MTIRFEEKVSTENAQFVCQWSNSLGKSFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVGFIQKIRLEDSNLHIGRFFINPQKQEQGLGSKALRKFVSLAFE
                     NRDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVRKYSRLKLE"
     gene            3106..3765
                     /locus_tag="VW411_001964"
     CDS             3106..3765
                     /locus_tag="VW411_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEU9794619.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTPLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELSLGLLFLLYSWGWLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTFQLILIASSGWNLVMVSFLILGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLATCLIIFGKLLL
                     V"
     gene            complement(3817..4968)
                     /locus_tag="VW411_001965"
     CDS             complement(3817..4968)
                     /locus_tag="VW411_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEU9794620.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNIIIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(4983..5714)
                     /locus_tag="VW411_001966"
     CDS             complement(4983..5714)
                     /locus_tag="VW411_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794621.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WTRPNHIDTAFASADLFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFP
                     LVQKNKSGYLFEYAQMNTLYLNQHSSYKNDENSHSFPIQMELVSDEMIQEIEQATKNS
                     NIEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDI
                     PNFSELSETEDFEIIPRIEKQEGQK"
BASE COUNT         1785 a         1028 c          957 g         1952 t
ORIGIN      
        1 aaattatata ataataacaa tcgaaataat tgacttttct atattaaagt tatataatag
       61 taataatcaa agaaattgat tttttgatat taaaataaaa aaggagggta ggcagtgttg
      121 tgatcaatta ttgctggagg tcttattggt ctcttggcag gtaaaatcac taaaaaagta
      181 gttctatggg aatcatcgca aatgtattcg ctggtttagt cggggcatat gcaggacaat
      241 ctcttttagg tagttggggt ccagcaatcg ctggaatggc tttgctccca tctattgtag
      301 gtgcagcgat tgtgattact gtagtgtcat tctttacagg tagaaagtaa acttttcgcc
      361 agtaaagtta gcaaactatt tttaaatcaa tgacgggaaa aatagtttaa atgttaaatc
      421 gaaaggattg tatatgtcaa aagcaaagaa aatatgtttc attattttct gtattttaat
      481 cttgacaatt ttccttcctg ttttgataga ttatcatcaa gttagtgatc taggtattca
      541 tctacttagc tggagacaga actccgtagt tgaattctat cttgctagat atgtcttttg
      601 ggggacagtg gttctatcaa ctttagtttt attatccatt ttagttgtga tgttttatcc
      661 taaacgttac ttggaaatcc aacttgaaac taaaaacgat acattaaaat taaagaattc
      721 ggcaatcgaa ggttttgtta gaagtttggt gagtgatcat agattgatca agaacccaac
      781 tgttcatgta aatttacgaa aaaataaatg tttcgttcat gtagaaggta aaattcttcc
      841 ttcagacaac atcgctgaca gatgccaaat aattcaaaat gaaataacta atggattgaa
      901 gcagtttttt ggtattgagc gtcaagtaaa acttgaagtt gcagtaaaaa attaccaacc
      961 aaaacctcaa aacaaaaaga ctgttagtcg tgtgaagtaa ggaagtaaaa aatggaatgg
     1021 cttaaacaat atcgatatcc aattatcgct ggtctcatag gcgtatttct ggcttgtttg
     1081 attgtctcct ttggcttctt caaaacaata tttgtattga ttttaggagc actgggagtt
     1141 gcagctggat tatatatcga aaaaaactat atagataaat aaaaaaataa aaattactaa
     1201 tttaattaaa ggagtttcat atgtcaaacg aaaaaaacac aaacactaac gtagaaaaga
     1261 aagatgctac tgttgtagct cacgaaatca aaggggaact tacttacgaa gataaagtta
     1321 tccaaaaaat cattggtctt tcactagaaa acgtttcagg tcttttggga atcgatggtg
     1381 gtttcttctc aaatcttaaa gaaaaaatcg ttaacagcga tgacgtaaca agtggtgtta
     1441 acgtagaagt tggtaaaaca caagttgcag ttgacttaaa cgttattgtt gagtaccaaa
     1501 aaaatgttcc agctttatat tcagaaatca gagaaatcgt atcttcagaa gttgctaaaa
     1561 tgactgactt ggaaattgtt gaaatcaacg taaacgttgt cgacatcaaa actaaagaac
     1621 agcatgaagc agactcagta agccttcaag atcgcgtatc tgacgttgct gaatcaacag
     1681 gagaattcac ttcagaacaa ttcgaaaaag ctaaatctgg tcttggatct ggtttctcaa
     1741 ctgttcaaga aaaagttagc gaaggtgtag aagctgttaa aggtgcagca aatggtgtag
     1801 tatctcacga aaacactcgt gtaaactaag ataaaataaa tataacagga gaaattatca
     1861 tgtcagtaga agaaaaatta aatcaagcta aaggttctat taaagaaggt gttgggaaag
     1921 ccatcggtga tgaaaaaatg gaaaaagaag gtgcagctga aaaagttgtt tctaaagtaa
     1981 aagaagttgc cgaagacgct aaagacgctg tagaaggtgc tgtagaaggt gttaaaaaca
     2041 tgttgagtgg cgacgataaa taaggttaaa agttacttta tctttttagt aatattagtc
     2101 aaaagagtct gagtcaagat gattctcaga aaacaaaaag ctagagattc ccaattgcgg
     2161 aactctagct ttttaatttt gcctctttct cttattatat ttcagcaggt tgttggccat
     2221 gagtacgaat cccatgtcaa ttctcacttg acgcttacct ctcagatgac atctcttata
     2281 acccaaacaa acctttatct gcccaaagac agatttcata tcaatcttac gtttagcgaa
     2341 aatttgtcta cccttggaag ataaaagtgc ctgatattct ttagttttta aacactggta
     2401 acgttcattc atatacagtc tcttttgagg ggctgattca ggttcataat cgcagtcaac
     2461 attgatttca aggctgtttg ctttctatct cccctttttc ataatgtata ataaaatgaa
     2521 ataataacag gacgaattga tcgggacagt caaatcgatt tctaacaatg ttttagaagt
     2581 agaggtgtac tattctagtt tcaatctact atattttcgt acaggtgctt caaccatttg
     2641 aacgatttca aatccttctt tttggtaaag attctgagct ctttgatttg cctcgaagac
     2701 atttagagaa atactgtcta tatctctatt ttcaaatgct aaactaacaa atttccttaa
     2761 agccttgcta cctaagcctt gctcctgttt ctgggggttg ataaaaaatc tcccgatatg
     2821 aagattgctg tcttctagcc tgattttctg gataaatccc acaaactctt gttcatcaaa
     2881 gattgaaaag actccttcca aggcttgaag tgtcagtaga aaaggaatcc ttggtcccat
     2941 ccattgttct tgaaaggatt tgcctaggga gttggaccac tggcatacaa attgagcgtt
     3001 ttctgtgctc accttttctt caaaacgaat tgtcatcttt tcctcaccac cttatctatg
     3061 tttctccatt atactatttc tcccattttt tacgaataga taagtatgat tgatttttat
     3121 ttttttctcg tcgggagcat tctagcttcc tttcttggtt tggtcattga ccgttttcca
     3181 gagcaatcca ttatcagttc agccagtcac tgcgattcct gtcagactcc cttgcgtccc
     3241 ttagatttga ttccgattct ctcacaggtc ttcaatcgct ttcgctgtcg ctactgcaaa
     3301 gttcgctatc ctgtctggta tgccctcttt gaattaagct taggactcct ctttctgctt
     3361 tactcttggg gatggctctc cttggggcaa gtcgtcctaa tcaccgctgg tttgaccttg
     3421 ggtatctacg actttcacca tcaggaatat cccttactgg tctggatgac tttccagcta
     3481 atcctaatag cttcctctgg ctggaatctg gtcatggtct ccttcctcat acttggaatt
     3541 ttggctcatt ttatcgatat ccgcatgggt gcaggggatt tcctcttttt agcttcttgt
     3601 gctctcgtct ttagcgtaac ggagttactg atcttgattc agttcgcttc tgcgacgggt
     3661 atcctggcct ttctcctgca aaagaaaaag gaaagacttc ctttcgtgcc tttcctctta
     3721 cttgctactt gtttgattat ttttggtaag ctactgcttg tctgataaaa tccaatttct
     3781 gccatatatc cttcatgaaa ttatttcaca gttaaattat aaattatttc ttttgtacaa
     3841 agggatgatg ttatcaaatc gatctgttct tctatcttct tgatactgat caaaaaattt
     3901 catttcgact gaaaatattt cgcttataaa ctgtaaacga atactttgtt tagacattat
     3961 agtcgctaga ctgactagat gattactcaa aacgacgtcc agaatactct ttactttgct
     4021 tggtttttta acaaaaattt gatcatccaa gggttcaatc attttgtaac ctttttgcgc
     4081 aatttgacga taaaagtaag aatgttgctt tggagtcaat aatcctaact taaaagctcg
     4141 atactctaaa gcctgtatcg aaacattcaa atccgacttc aataaaatat aactatcagg
     4201 attgctgaca cgcttgccaa ccctctcttc aaatttgact aaaaactctt cttttggcaa
     4261 taaaaaacat gatgcaaaat aatttgcttc ttgctccaaa cgatcgccat cttcattcat
     4321 atctttatat ttatgtaaaa gaatatgtcc tagctcatga gctaagtcaa aatttcgacg
     4381 tacagatgat ttattcgttc ctaacacaat ataaggtctt cccaattttg accatgcgct
     4441 ataagcatca gcttggccat taattaatcg ttccacgata tagatgcctg aacgttctaa
     4501 tttataaagc aaatcatgat tatcttttga aatacctaat ttttccctgg cataaagagc
     4561 caattcctca atggattctc ccttatgata agattcactc actacattac ttaggtcatg
     4621 aattataata ttaggtataa ttacaaaact ttcaaaataa tcaatcaaac tatctacctt
     4681 atgtaaatac atagtttgaa tatctattgt tttccgtgtt gctaggtctg catttctaaa
     4741 ggcaattaca gaagaatcaa atcgaatgct ctcttcttcc tgttcaaaat aagttaaatc
     4801 aacatgaaat tggttggcca aatgcatttt ggttgataat ttaggtttcg tttcgttgga
     4861 ctcaaactgc caaatggctt gttccgttaa attaattctc tgagctaatt ctgctctact
     4921 taaaccattt aacagccgta attctttcaa tacccgacca ttaaacattt acatactcct
     4981 tactactttt gaccttcttg tttttctatt cttggaataa tttcaaaatc ttctgtttcc
     5041 gataattctg aaaaattagg aatatcttga tatttagctt cttcgaaatg gtacgaactt
     5101 gattgaatat actcagacaa atcttgaatc aagtgtaact gaccagttcg tgcatcaggc
     5161 ataacaacat ctacagatat aatattgttt tctgagtccg cctcataagt taaaatcata
     5221 aatttttcga tattcgaatt tttagtagct tgttcaattt cttgaatcat ttcatcagaa
     5281 actaactcca tctgaattgg aaaggaatga ctattttcat catttttgta ggaagaatgt
     5341 tgattaagat aaagtgtatt catctgagca tattcaaata agtagccact cttatttttt
     5401 tgtaccaaag gaaattggtt tgtaagtcgc ttcttaccct ttataattaa caatactttc
     5461 ccatattttt ctgtatttgt ttcaaattct aaatatcccc aagtctgtcc tgctaattgt
     5521 aatttatact caaacaaatc tgctgatgca aatgcagtat caatatgatt aggtcgcgtc
     5581 catgcataac cattcgacac tatcattgtc tctctttttt ctagacgttc atctacataa
     5641 tctttttgcc ctttcatcaa agtatctaca attttttgtg cctcaagcga atcaaagaga
     5701 tcctgattca acataattct tc
//
LOCUS       DAVKNL010000088         5651 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVKNL010000088 DAVKNL010000000
VERSION     DAVKNL010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.088"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(<1..210)
                     /locus_tag="VW411_001967"
     CDS             complement(<1..210)
                     /locus_tag="VW411_001967"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794622.1"
                     /translation="MNTIERTRRLIKGCATHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     SKHVFEVVDETNEVSSKHVFEVVDET"
     gene            complement(274..1284)
                     /gene="ccpA"
                     /locus_tag="VW411_001968"
     CDS             complement(274..1284)
                     /gene="ccpA"
                     /locus_tag="VW411_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEU9794623.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(1709..2308)
                     /locus_tag="VW411_001969"
     CDS             complement(1709..2308)
                     /locus_tag="VW411_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9794624.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLMLQPDVESVFQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRAEKLETKVVVVTTFKRPGYFERAVKAGVDAYVL
                     KERNIADLMQTLHTVLEGRKEYSPELMEVVMMHPNPLTEQEIAVLKGIAQGFSNQEIA
                     DKLYLSNGTVRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(2309..3406)
                     /locus_tag="VW411_001970"
     CDS             complement(2309..3406)
                     /locus_tag="VW411_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9794625.1"
                     /translation="MLERLKRIHYMFWISLIFMIFPILSVVTGWLSAWHLLIDILFVV
                     AYLGVLTTKSQRLSWLYWGLMLTYVVGNTAFVAVNYIWFFFFLSNLLSYHFSVRSLKS
                     LHVWTFLLAQVLVVGQLLIFQRIEVEFLFYLLVILTFVDLMTFGLVRIRIVEDLKEAQ
                     VKQNAQINLLLAENERSRIGQDLHDSLGHTFAMLSVKTDLALQLFQMEAYPQVEKELK
                     EIHQISKDSMNEVRTIVENLKSRTLTSELETVKKMLEIAGIEVQVENHLDKSSLTQEL
                     ESTASMILLELVTNIIKHAKASKVYLKLERTEKELILTVRDDGCGFASISGDELHTVR
                     NRVFPFSGEVSVISQKHPTEVQVRLLYKERK"
     gene            complement(3406..4143)
                     /locus_tag="VW411_001971"
     CDS             complement(3406..4143)
                     /locus_tag="VW411_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9794626.1"
                     /translation="MKRWLALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GELANFMRDYLISMTAFSMMSTAIFSFPVVLHTDKINNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFSVGHFVRGVDMSLGNWIGAALLLIVGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQVVGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSVVLFGLTIYLQGHKENA"
     gene            complement(4145..5029)
                     /locus_tag="VW411_001972"
     CDS             complement(4145..5029)
                     /locus_tag="VW411_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9794627.1"
                     /translation="MNMIKVESLNKNIKGKAILKGISFEVAEGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLSVTSSQLDYIRGYLPQENVIVQKLKVKELIAFFQRIY
                     PNSLSDQEIDQLLQFDQQQKEQFAEKLSGGQKRLFSFVLTLIGRPKLVFLDEPTAAMD
                     TSTRQRFWEIVRDLKAQGVTILYSSHYIEEVEHTADRILVLNKGELIRDTTPLAMRSE
                     GIEKHFILPLAYKEVIEQSNLVENWSQKQDALQVVTREADAFWELLVQAGCGIQEIEV
                     NNRSLLDTIFEETQKGDN"
     gene            complement(5016..5210)
                     /locus_tag="VW411_001973"
     CDS             complement(5016..5210)
                     /locus_tag="VW411_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794628.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTGWFDQTLLRQFIRGALGFGFM
                     IFVVFLMRMEWLKGEYHEYD"
     gene            complement(5207..5338)
                     /locus_tag="VW411_001974"
     CDS             complement(5207..5338)
                     /locus_tag="VW411_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine aminotransferase"
                     /protein_id="HEU9794629.1"
                     /translation="MFSGLDESFYHFPWELFAGFGMMSWLVREGLKLVGDVKKELEE"
BASE COUNT         1779 a         1295 c          931 g         1646 t
ORIGIN      
        1 agtttcatct acaacctcaa aaacatgttt tgagctgact tcgttagttt catctacaac
       61 ctcaaaaaca tgttttgagc tgacttcgtc agttctatct gcaacctcaa agcagtgctt
      121 tgagctgact tcgtcagttc tatctacaac ctcaaaacag tgtgttgcgc agcctttaat
      181 cagccgccta gtccgctcta tggtattcat taagtcaaca tctcttgttt aagagcacca
      241 aatcaggaaa tcttctcgat tccctgattt tttctattta cgttttcgtg ttgagctacg
      301 ttctgtcaaa ccatgaggta agagaacttc acgttcttcc aactcttcct tatgcataat
      361 cttggtcaac atacgcatac taatggcacc aaggtcataa agaggttggg caatcgttgt
      421 caagtttgga cgggtaaagc gtgagatttg tgaatcatca ctagtaataa tttcaaaatc
      481 ttctggcaca gaaacaccct tatcagccaa accgttcaag actcctgctg ccaactcatc
      541 acctgtcaca actgctgcag ttgcatttga tgaaatcaaa cgctctgcta aggcgtaacc
      601 atcatcatag ctatatttag attcaaatac caaaccctca ctataagtga ttcctgcttt
      661 tttcaaggtt tccttgtagc caactaaacg aaccttacca ttgatgtcat ccactagcgg
      721 accgctaacg aaagcaatac gctcattttc tttagcaagg taactcactg catcaattgt
      781 tgcttgctta tagtcaatat tgacacttgg caactggtgc tcaacatcga cagttcctgc
      841 gagaacaatc ggagtacgtg aacgcgaaaa ttctgagcga attttatctg tcaagtgata
      901 ccccatatag ataatgccat ctacctgctt tgaaaagagg gtattgacaa cagaaacttc
      961 tttctcgtta tcttcatcgc tattagctag gacaatattg tacttgtaca tttctgcaat
     1021 atcatcaatc cccttagcca aactcgaaaa ataaccattg gtaatatttg gaatcacgac
     1081 accgacagtg gttgtctttt tacttgcaag accacgcgca actgcatttg gacgataatc
     1141 caaacgatca attacctcta gcactttttt acgggtattc tcttttacat ttttattgcc
     1201 attgaccaca cggctgaccg tcgccatgga aacacctgct tcacgagcga catcataaat
     1261 ggttactgta tcatctgcat tcattccttt tcctgtcctt tctatctcac acattctttt
     1321 acaagtagag gtactgattg aagctctata tctacttaca aaagtgaaga tgtgaaaatt
     1381 tcgttttcat atttctactt attccattct atcactaatt gtaaacactt tcaagtgttt
     1441 tttgaagatt gattgaaaaa atttcataga aaacctaggt ttagctcctt gctaccacct
     1501 tagactaaac aaaaaggagg aaactaagcc ctcctaaagt tatagtaaaa tgaaataaga
     1561 acaggataaa tcgatcagga cagtcaaatc gatttctaac aatgttttag aagtagaggt
     1621 gtactattct agtttcaatc tactataggt attgttccat tcactaccgt caattttagc
     1681 acatagtctt catgaaaata ttatatcatc ataaccaacc agattctttc gcgatattag
     1741 ctgcctctgt tcgattacct gcatctagtt tcgaaagaat attggtgaca tagtttcgga
     1801 ctgttccgtt ggatagataa agtttgtctg caatttcttg gttagagaag ccctgagcaa
     1861 ttccctttaa aactgcgatt tcttgctccg ttaatggatt gggatgcatc atcaccactt
     1921 ccatcaattc aggcgaatac tccttgcgtc cttcgaggac ggtgtgcaag gtttgcatga
     1981 ggtctgcaat gtttctttct tttaatacat aagcatctac tccagccttg accgcacgtt
     2041 caaaataccc aggacgcttg aaggtcgtca ccacaaccac ctttgtttca agcttttctg
     2101 ctcgtatcca ctccaagact tcaagacctg tcttaacagg catttctacg tcaaggatgg
     2161 cgatatctac agactccttt tctaatagtt ggattgcttc ttgcccattc ttggcttgaa
     2221 agacagactc tacatccggt tgaagcatga gcaactggca catggcatct cgcaacatac
     2281 tttgatcttc tgcgactaat actttcatct actttctctc cttataaagt agtcgaacct
     2341 gcacttcagt tggatgtttc tgactgatta cacttacttc tcctgaaaat ggaaaaacac
     2401 gatttcggac tgtatggagc tcatccccgc ttatagaggc aaagccacag ccatcatctc
     2461 tcactgttag aatgagttct ttctctgtcc gttctaattt caagtagact ttagacgctt
     2521 tagcatgttt gatgatattg gtcactaatt caagcaaaat catggaagcc gttgactcca
     2581 attcctgagt taagctagac ttgtccaagt gattctcaac ttgaacctca attccagcaa
     2641 tttctaacat ctttttcaca gtctctagtt cggatgtcaa agttctagac ttaagatttt
     2701 ccacaatggt tcgcacttca ttcatggaat ccttgctgat ctggtgaatt tcttttaatt
     2761 ccttttccac ctgtggataa gcctccatct gaaataactg caaggctaaa tctgtcttga
     2821 cactcagcat agcaaaggta tgtcccagac tatcatgcaa atcctgaccg atacgactac
     2881 gttcattttc agcaagcaat agatttatct gagcattttg cttgacctga gcttctttca
     2941 aatcctcgac aatacgaatc cgaaccaatc caaaagtcat taaatcgaca aaagtaagaa
     3001 ttacaagtag atagaataga aactcaactt cgattctctg aaaaatcaac agttgcccca
     3061 caacaaggac ttgagcaaga agaaaagtcc agacatgtaa agactttaaa ctacgtacgc
     3121 tgaaatgata acttaagaga ttggatagga aaaagaaaaa ccagatataa ttaacagcaa
     3181 caaaggcagt attcccaact acataagtca gcatgaggcc ccaatatagc caagataggc
     3241 gctggctctt agttgttaaa acacccaaat atgccactac aaatagaata tcaatcaata
     3301 aatgccaggc agaaagccac ccagtcacta cagacaggat ggggaaaatc ataaaaatta
     3361 aactgatcca aaacatataa tgtattcttt tcagtctttc aagcattaag cattctcctt
     3421 atgaccttga aggtaaatgg tcaaaccaaa caaaactact gaaaaaacaa gtaaataaac
     3481 tgtggctgat agattgatgc caccctcatt taagaaggtc ttgagcaact ccatcaactg
     3541 ataggtcggg agacacttac ctactacttg catccagtct ggaaataaag agataggcat
     3601 ccagagtcca cctaaaacag ccaaccctag ataaagaaga ttgcccacga cagacatcaa
     3661 ctgactagtt ggtaagagag tcaaggtcaa accaagcgct acgaaggcaa tacttcctac
     3721 tatcagcaaa agtgcagccc caatccaatt tccaagagac atgtccacac ctcttacaaa
     3781 atgcccaact gagaaaacca ccaagattga aaccaaataa tcaaccagca tacttgttat
     3841 ctttgataga taatattcta ccatatttac agggctatga cgcaatgttt tctgccagtt
     3901 gttgatcttg tcggtatgta aaacaactgg gaatgagaag atagctgttg acatcatgga
     3961 aaatgcagtc atggagataa gataatcacg cataaaattc gcgagttcac ctggtgtgtc
     4021 ctgatagata ccagaaaaaa ataaatagaa agccgtcggc atccctactg acaatagata
     4081 atagatcaat tgtcgtttgg tcaataaaaa ttctatctta ctaagtgcta gccatcgttt
     4141 catcttagtt atctcccttc tgcgtttctt caaagattgt atccaacaaa ctacgattat
     4201 taacttcaat ttcttgtatg ccacatcctg cttgaactaa cagttcccaa aaagcatctg
     4261 cttcgcgtgt gactacttgt agagcatcct gtttttgtga ccagttttca accaagttag
     4321 actgctcaat gacttccttg tatgccagag gaaggataaa atgcttttca attccctcac
     4381 tacgcatagc tagaggcgtc gtatcacgaa tcaactctcc cttatttaaa accaaaatcc
     4441 ggtcagccgt atgctctacc tcttcaatat aatgagacga atagagaatc gtgactcctt
     4501 gcgcttttag gtcccgaacg atttcccaaa agcgttgacg agttgaagta tccatggcag
     4561 cagttggttc atctaaaaag acaagctttg gtcgcccaat caaggtcaag acaaaagaga
     4621 agagacgctt ttgcccgcct gacaattttt ctgcgaattg ctctttttgt tgctggtcaa
     4681 actgcaatag ttgatcgatt tcctgatcgc tcaaggaatt tggatagata cgttgaaaga
     4741 aagcaatcaa ctctttgacc tttaatttct gaacgatgac attttcttga ggcagataac
     4801 ctctaatata gtctaactga gaactcgtca ctgacaagcc ttggatggat acttgaccgc
     4861 ttgtgaccag tttatctcca agcagacagt ccaagagtgt ggtcttccca gcaccattgg
     4921 gcccaatcaa ggcgacgcat tcaccttcag ctacctcaaa ggaaataccc ttcaaaatag
     4981 ccttgccctt gatgttttta tttaggcttt ctaccttaat catattcatg atattctcct
     5041 ttcaaccact ccattctcat aaggaaaacg acgaaaatca taaatccaaa ccccaaagca
     5101 ccacgaatga attggcgaag caaggtttgg tcaaaccaac ctgtaaacat ttccactaac
     5161 cataccaaga gtgacaggcc gataaagaaa tagatgatcc ctctcttcat tcctcaagct
     5221 cctttttcac atctccgact aatttcaaac cttctctaac aagccaagac atcattccaa
     5281 agccagcaaa gagctcccaa ggaaaatgat agaaactctc atccaatccc gaaaacatga
     5341 gttaggtcat aactcctgct actactaaac tcactgcgat aatcatttta tttctcatct
     5401 cttcttcctc catttcatac tacaattata gtcttttgaa atcagaggag acagaagctt
     5461 ctgtcactag aaaatatgac aaatgtcata aaaaattctg ttcaaaacaa gcaagataca
     5521 ctatacaata aaacacaatt agaaaaatct aaggcaactt cctcaaaaga gatatcaaac
     5581 ccaattcaca ccataatgta aactaatact tatttaaaat caaaaagagt agaaattttt
     5641 atcagacaaa c
//
LOCUS       DAVKNL010000089         5511 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVKNL010000089 DAVKNL010000000
VERSION     DAVKNL010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5511
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.089"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            107..1630
                     /locus_tag="VW411_001975"
     CDS             107..1630
                     /locus_tag="VW411_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016648332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9794630.1"
                     /translation="MFSHILGFRLETNLRKRGIDGLEKASFRFFDLNPSGQIRKIIDD
                     NAAQTHQVVAHMIPDSSQAIITPVLVLALGFIVSIRVGIILLALTIIGGLILGAMMGE
                     QEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFKSFYKAIKDYSKYAYDYSLS
                     CKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLELIMILFLSGVLFVSFMRMM
                     WYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNYNIEFENVSFAYNDKAVIEN
                     LSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSIKIGGIAISEYSDEALIKAI
                     SFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGCDLILDKFPERENTIIGSKG
                     VYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEFELQKAFKNLMKDKTVIMIA
                     HRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSLQEMFNSANEWRVSNERVL"
     gene            1614..3335
                     /locus_tag="VW411_001976"
     CDS             1614..3335
                     /locus_tag="VW411_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9794631.1"
                     /translation="MKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLILIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLYKKMEDSEKVHLKAEVTTILTLSISSIFSFISLAVVIFVGVNLIINKEINSLYLI
                     GYLLAAMKIKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKFDIDLKD
                     VEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQILIDGK
                     DIKEISTESLFDKVSIVFQDVVLFNQSVMENIRIGKQDASDEEVKRAAKLANCTDFIE
                     KMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNEKKIQES
                     LNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNLIEKTK
                     MAEEFIY"
     gene            3346..3975
                     /locus_tag="VW411_001977"
     CDS             3346..3975
                     /locus_tag="VW411_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU9794632.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALVALGAFLGGILGAYIGKALLKKHFSNGLYCVGYFTPCLILWCYLN"
     gene            3876..4433
                     /locus_tag="VW411_001978"
     CDS             3876..4433
                     /locus_tag="VW411_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9794633.1"
                     /translation="MYRQGSIEKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIP
                     IVIRMFILPFMAASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKN
                     IKMAMRVRGINFKNPVKYLEYVSVPLLIISSNISDDIAKAAETKAIENPIAKTRYIRV
                     KIQLIDFVYVLAVAGLIVGGLIWLK"
     gene            4421..>5511
                     /locus_tag="VW411_001979"
     CDS             4421..>5511
                     /locus_tag="VW411_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9794634.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGNGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDILEIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDEPSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLIDKGKLK
                     KTYTRSEFLKLDKNELNALSLRDKELSKLKVPYLKEGGEYQIKNLSYKFTDDECLSLK
                     DISFKLGKIYGIIGSNGRGKSTLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVM
                     QDVNHQLFTDEVFNELRLGVKNFDEEKAK"
BASE COUNT         2015 a          701 c         1047 g         1748 t
ORIGIN      
        1 tctagataag ttaataatta attcaaactt atccggtgca gagagtatag cattaaaatc
       61 tgttattaca ctaacaagtg gagcgatatt ttattttgtc tcaggaatgt tttcacatat
      121 cttgggattc aggcttgaaa caaatttaag aaaaagggga atcgatggtc tggaaaaagc
      181 aagttttagg ttctttgact taaatccatc tggtcaaata agaaagatta tagatgacaa
      241 tgctgcacaa actcatcagg tggtagcaca catgattccc gatagttctc aggcaataat
      301 cacacccgta cttgtacttg cacttggctt tatagtaagt ataagagttg gcataatttt
      361 gcttgctctt actataattg gtggcttaat tttaggggca atgatgggcg agcaagaatt
      421 tatgaagata taccaagaat ccctatctaa actaagtgct gaaactgttg agtacgtgag
      481 aggaatgcaa gttgtaaaaa tatttaaagc aaatgtagag tcttttaaaa gcttttataa
      541 ggcgataaaa gattactcaa agtatgctta tgattattcc ctatcttgta aaaggcctta
      601 tgttttgtat caatggttat tttttggact gattgcaatt ttaattattc ctatagttta
      661 ttttatgact agcttagcta gcgcaaaggt gattttactt gagcttatca tgattttatt
      721 tttatcagga gttctctttg tttcattcat gagaatgatg tggtactcca tgtatatttc
      781 tcaaggaaat tatgcagtag atactttaga ggcgctttac gaagatatgc aaaaagacaa
      841 attagtgcat ggtaatgtca ataattttaa aaactataat atagaatttg agaatgttag
      901 ctttgcttat aatgataaag ctgtcattga aaatttatcc tttaatttag aagaaggaaa
      961 gtcctacgca cttgtcggtt catctggatc aggcaaatca acagtagcaa aacttatatc
     1021 aggtttttac aatgttaata aaggaagcat aaagataggc gggatagcaa taagtgaata
     1081 ttctgacgaa gccttaatta aagccatttc ctttgttttt caagattcaa aattattcaa
     1141 gaagagcatt tatgataatg tagcgttagc taataaagat gcgacgaaag atgacgttat
     1201 gagagcctta aaattagcag gatgcgattt aatattagac aaattcccag aaagagaaaa
     1261 tacaatcata ggctcaaaag gtgtttattt atccggtgga gaaaaacaaa gaattgcaat
     1321 tgctagagca attttaaagg attccaaaat tattattatg gatgaagcat cagcatctat
     1381 tgacccagat aacgagtttg aattgcaaaa agcttttaaa aatcttatga aggataaaac
     1441 agttatcatg attgcacaca ggctatctac aattaaagac cttgatgaaa ttattgtcat
     1501 ggatagtgga aaaattatag aaagagggtc tgacaaagaa ttaatgtcaa aagatacaag
     1561 gtataagagc ctgcaagaga tgtttaacag tgcgaatgaa tggagggttt caaatgaaag
     1621 agttttataa aaaaagattt gctcttacag atggaggagc aagaaattta agtaaagcaa
     1681 cactggcttc atttttcgtt tattgtataa acatgcttcc tgccatatta cttatgattt
     1741 ttgctcagga agttttggaa aatatgggca aaagcaatgg cttttatata gtattctcag
     1801 ttttgatttt gatagcaatg tatattttgc tttctatcga atacgataaa ttatataaca
     1861 caacctatca agaaagtgca gatttaagaa taaggacagc ggagaattta tcaaaattac
     1921 ctctatctta cttttctaaa catgacattt ccgacatttc acaaacaatc atggctgata
     1981 ttgaaggcat agagcatgca atgagccact caataccaaa ggtgggcggc atggtactgt
     2041 ttttcccatt aatatctgta atgatgctag cgggcaatgt caagatgggt ttagctgtaa
     2101 ttattccatc tattttaagc tttatattta tacctttatc taaaaaatat caggttaatg
     2161 gacagaatag atattatgat gtcttaagaa aaaactcaga aagctttcaa gaaaatatcg
     2221 aaatgcaaat ggagattaaa gcatataatt tatcgaagga tattaaagat gacttatata
     2281 aaaaaatgga agatagtgag aaagtacact taaaggcgga agtaactaca attttaactt
     2341 tgtctatatc ttcaatattt agctttatat ctcttgctgt tgtgatattt gtcggcgtaa
     2401 atctaattat taataaagag ataaattctc tctaccttat aggatattta ctagctgcta
     2461 tgaagataaa agactcttta gatgcatcta aagagggctt gatggaaata ttttatttat
     2521 cgcccaaaat agaaagatta aaagaaattc aaaatcaaga tttacaagaa ggcgatgact
     2581 atagcttaaa aaaatttgat attgatctaa aagatgttga gtttgcctac aataaagacg
     2641 caaaagtttt aaatggtgta agttttaaag ctaagcaggg agaggtcact gctttggtag
     2701 gtgcaagtgg ctgcggtaaa acaactatct tgaaacttat atcaagactt tatgattatg
     2761 acaagggaca aatcttaatc gatggcaaag atataaagga aatatcaaca gaatcccttt
     2821 ttgataaggt gtctattgtt ttccaagatg tggttctctt taatcaaagc gttatggaaa
     2881 atattagaat cggtaagcaa gatgcaagtg acgaagaggt taaaagagca gcaaaacttg
     2941 caaattgcac agattttata gaaaaaatgg ataaaggttt cgatacagtt attggtgaaa
     3001 acggagctga gctatcagga ggagaaagac aaagattatc aatagccaga gccttcttaa
     3061 aagatgcgcc gatattgatc ttagatgaga taacagcaag ccttgatgtt aacaacgaga
     3121 aaaagattca agagtcttta aataatttag ttaaagataa aactgttgta atcatttcac
     3181 atagaatgaa atccatagaa aatgcagaca agatagtagt tcttcaaaac ggaagagtag
     3241 aaagcgaagg taagcatgaa gagcttttac aaaaatcaaa aatttacaaa aatttaatag
     3301 aaaagacaaa aatggcagaa gaatttattt attaggagga ctacaatgga taataaaaaa
     3361 ttaaaagtaa aagatttagt aagcatcggt gtttttggcg taatttattt tgccttcatg
     3421 tttggagttg gtatgatggg cttgattcca atattgttct taatataccc gacagtatta
     3481 gccatagttg caggaactgt tgttatgtta tttatggcta aggttcaaaa gccatgggca
     3541 ctatttatat ttggtatgat atcaccactt gtgatgtttg cagctggtca tacctacgta
     3601 gttgtggttt tatcacttat agtaatgata atagcagaat taattagaaa gattggtaat
     3661 tataattcat ttaaatacaa tatgctttct tatgcaatct tcagcacatg gatatgtagc
     3721 tctttaatgc aaatgctttt agcaaaagaa aaatatatgg agtggtcttt gatgactatg
     3781 ggaaaagatt atgttgatgt attagaaaag ttaataactt atcctcacat ggctttagta
     3841 gccttaggtg ctttcttagg aggaattctt ggagcatata taggcaaggc tctattgaaa
     3901 aaacactttt caaatggatt atattgtgtg ggatacttta ctccttgcct aattttatgg
     3961 tgctatctga attaaaccct atagttaaga tgtttttgag tatacctatt gttattagaa
     4021 tgtttatttt accatttatg gcagcaagct ttatgataaa gacctcggat gtaggcgcaa
     4081 taatttcatc gatggataag cttaagattt caaagaatgt atccatacct attgcggtta
     4141 tgtttagatt cttcccatct tttaaggagg agaagaaaaa catcaaaatg gctatgagag
     4201 taagagggat aaattttaaa aacccagtca aatatcttga atatgtttct gtgccactac
     4261 tcattatatc atctaatata tcagatgaca ttgcaaaagc ggcagaaaca aaggcaatag
     4321 aaaatccaat tgccaagacc agatacattc gcgtaaagat acagctaatt gattttgttt
     4381 atgttttagc ggttgctgga cttattgtgg gaggcttaat atggttgaaa taaaaaattt
     4441 aagtcttgat tatggtgaag agcatatatt agatgatata tcactatcca tagccgaggg
     4501 agagtgcgtg ctatttacag gaaaaagtgg aaatggtaag tcatctttaa taaattcaat
     4561 caatggacta gctgtaaggt atgataacgc aaagacaaag ggcgaaataa ttattgatgg
     4621 taagaatata aaaaatttgg aactttatca aatctcaatg cttgtttcaa ctgtttttca
     4681 aaatcctaag acatattttt ttaatgtcaa tacgacatta gaattattat tttatttgga
     4741 aaatatcggt cttgcaagag aagagatgga caggcgtttg aaggatatac ttgagatatt
     4801 cccgataaaa aatcttttga acagaaatat atttaatcta tccggcggtg aaaaacaaat
     4861 tctttgcatt gcagcttctt atatagcagg tacaaagatt atagttatgg atgagccttc
     4921 atcgaattta gatattaaaa gcataagtgt tttggcaaag atgctaaaga tattaaaaga
     4981 gaaaggcata agcataattg ttgcagagca tagaatttat tatttgatgg acatagttga
     5041 ccgtgtattt ttaatagata aaggaaagct taaaaaaact tatactagaa gtgaattttt
     5101 aaagctagat aaaaatgaat taaatgcttt aagtttaaga gataaagaat taagtaaatt
     5161 aaaagttcct tatttaaaag aaggtggaga gtatcagata aaaaatctta gttacaaatt
     5221 tactgatgat gagtgtttaa gcttaaaaga tatttcgttc aagcttggga aaatttatgg
     5281 cataatagga tccaacggac gaggaaaatc aacgctttta agatgtttaa taggtcttga
     5341 gaaaaaatca aaagaagaaa tttattttaa gggagagaag ctatctaaaa aagaaagact
     5401 caaaaactct tcacttgtta tgcaagatgt aaatcatcaa ttattcacag atgaagtatt
     5461 caacgagctt agattaggag taaagaattt tgatgaagaa aaggcgaaaa t
//
LOCUS       DAVKNL010000090         5498 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVKNL010000090 DAVKNL010000000
VERSION     DAVKNL010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5498
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.090"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            89..855
                     /locus_tag="VW411_001980"
                     /pseudo
     CDS             89..855
                     /locus_tag="VW411_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1687..2043
                     /locus_tag="VW411_001981"
     CDS             1687..2043
                     /locus_tag="VW411_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794635.1"
                     /translation="MKRDVRDIRKQFRLTEAEEKQILALMRERGETNFSDFLRKSLLS
                     SDLQKQMETWFALWQSQKLEQISRDVHEVLILAQSERQVTQEHVSILLTCVQELIQEV
                     ANTIPLSKEFREKYMR"
     gene            2048..2413
                     /locus_tag="VW411_001982"
     CDS             2048..2413
                     /locus_tag="VW411_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEU9794636.1"
                     /translation="MEHRYRTNLKKVFLSDSELNQLNINIDQSGCKSFSEYARRTLLD
                     PGMNFITIDTNGYQDLVFELKRIGNNINQIARSVNQSQLISGEELQELKKGIGELIKE
                     VDKEFNLQAQKLKEFHGHH"
     gene            2400..4223
                     /locus_tag="VW411_001983"
     CDS             2400..4223
                     /locus_tag="VW411_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002917262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEU9794637.1"
                     /translation="MVITKHFAIHGKSYRRKLIKYILNPEKTNNLALVSDYGMKNFLD
                     FPSYEEMVQMYHENFISNDTLYDFRHDRMEENQRKIHAHHIIQSFSPEDHLTPEQINR
                     IGYETVKELTGGKFRFIVATHVDKDHLHNHIIINSVDSNSDKKLKWDYKVERNLRMIS
                     DRFSKIAGAKIIENRYSHQRYEVYRKTNHKYELKQRLYFLMEHSRDFEDFKKNAPLLH
                     VEMDFRHKHATFFITDSTMKQVVRGKQLNRKQPYTEEFFKNYFAKREIESLMEFLLLK
                     VENMDDLLQKAKLFGLTINPKQKHVSFQFAGVEVKETELDQKNLYDVEFFQDYFKNRK
                     DWQAPETEDFVQLYQEEKLSKEKELPSDEKFWESYQEFKSNRDAVHEFEVELSLNQIE
                     KVVDDGIYVKVKFGIRQEGLIFVPNMQLDMEEDKVKVFIRETSSYYVYHKDAAEKNCY
                     MKGRTLIRQFSYENQTIPLRRKATVDMIKEKIAEVDALIELEVENQSYVTIKDELVHE
                     LAASELRINELQERMSTLNQVAEYLLASVESKQEMKLNLSKLNITENISANIVEKKLK
                     SLGNQLELERGRYEKMVVRLDKFINRLNTGLSKGDGIDFQK"
     gene            4337..5203
                     /locus_tag="VW411_001984"
     CDS             4337..5203
                     /locus_tag="VW411_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9794638.1"
                     /translation="MGTLLATRLKNRRKELKMSQRELAEGICKQGQISRLESGEFTPG
                     ADFLHALAKKLKVSMDYFFDEQVVEEVNELSEFKKLAQTFITNRNYESLKYIYELENV
                     KAHRLSLADKFYMEWIKSLIDFYFYGQKEEAITRLENMLPQLNVSDLNYLQVSNTLFN
                     FYYDIEDLERFNEIRDKLEHQVNQLNLNTIEELELSIKFNYNVCRYLWLQKNIEEAIT
                     KITATIKQCKEYRTTYLLADLYLLMGSVSENFSSKSSVKEYFETAHFLYKLEENMSMA
                     LKVEHYFADITE"
BASE COUNT         1975 a          809 c         1119 g         1595 t
ORIGIN      
        1 cataaaaaat ggaatagaaa tagttgtagt aactgatcat aatactacca aaggtattaa
       61 aaagttacaa atggcagtct caatcataat gaaaaattat ccgatttatg atatacatcc
      121 tcatatttta catggagtag aaattagtgc agcagataaa ttgcatattg tatgtatata
      181 tgattatgaa caagaatcat gggttaatca atggttaagt gaaaatatta taagtgagaa
      241 agatggaagt tatcaacatt cactgactat aatgaaggat ttcaataatc aaaaaatagt
      301 taactatatt gctcatttca atagttatga cattttgaaa aaaggttctc acttatcagg
      361 tgcatataaa cgaaaaattt tttctaaaga aaatacacga ttttggagtt taatattaac
      421 tcgaaagaat cttcgcaaca acttgatatt ctctataaag aagttggtgt attaagtttg
      481 ggacaaaaag ttgtagccat gcttgatttt ttattagcat atagtgatta ttctaaagac
      541 ttcagaccat tgattattga tcagcctgaa gacaatctag acaatcgtta tatttacagg
      601 catttagttc agcagtttag agatgtgaaa gctcaacgtc aaattatttt agcaacacat
      661 aatgctacaa ttgtaacaaa ttctatgaca gatcaagttg ttattatgga gtcagatgga
      721 gttaacggat ggattgaatc acagggatat gttagtgaaa aatatataaa aaatcatatc
      781 atcaatcaat tagagggagg aaaagattcc ttcaagcata aaatgtctat atatgagacg
      841 gctttatcag agtagagtca gaaaaagtag gttagaaatt tagcctactt ttttctttgt
      901 ccgacaggca tagtgtacat ctgaggtcca agtcctctgt ggatatttgc tgcagatgaa
      961 accaatagcg actcctaagc ctgaatatcg tgaggtaggg gggataggaa ggaattagcg
     1021 aaatcaaggt tctacaaaca gaatcgtgac ttgaagccat atatagcgga tgaggaactc
     1081 taaaatccaa ataggtgtcg taacctatat acgtaaatta cgagagtaaa ctaggaaaga
     1141 tgtacggctt attccgtgag cgtttaggac gtagtacaac gaatcatggg agtcagctga
     1201 acacatagta ttgaagaaat ttctgtaatg gaaatggagc gaagaagtga acaattaaat
     1261 gaatacctct ctaattaaat ttgtcaattc taattcctgg tatgaaaaga cagtgacctg
     1321 aaaatgtaaa cgatgggagc tgatcataaa tataggacgg tacatgcagt ggtgttagag
     1381 attagtcctt acttgatttg tgataacttc cccaaatttc ttctgctata cttttctcaa
     1441 cttttaaaaa tccaactaag aattttacct gggggtttgg gggcggagcc actaagttat
     1501 cttatcgtta gctgtcaaaa ctggtaggtt ttgataggct ggcgatatga tttttgggat
     1561 attgtggaca caatatctga gctcgcaaag ccttacaaga atgaaaatca gttgttggaa
     1621 aagtgtactg acattgtatg gtagctcaca ttgtcagtac aagtattttg gaaaggaagt
     1681 agcagtatga aacgagatgt gcgtgatatt cggaaacaat ttcgtttaac agaagcagaa
     1741 gaaaagcaaa ttctagcttt gatgagagag cggggagaga ctaatttctc tgattttctt
     1801 cgtaaaagtt tactttcctc tgatttacaa aaacagatgg agacatggtt tgccctctgg
     1861 caatcccaaa aactagaaca aatcagtcgt gacgttcatg aagttttaat cttggcacag
     1921 tcagaacgtc aagtcaccca agagcatgta tctattctct taacgtgcgt gcaggaattg
     1981 attcaagagg ttgcaaacac catacccctc agtaaagaat ttcgtgagaa gtacatgagg
     2041 taagcacatg gaacatcgtt accgaaccaa tctcaagaaa gtgtttttgt ctgatagtga
     2101 gttgaaccaa ctaaatataa atatcgatca aagtggttgt aaatcctttt ctgaatatgc
     2161 gagacgaact ctactcgatc ctggtatgaa ttttatcacg attgacacaa acggttacca
     2221 agatttagtg tttgagttaa agaggattgg caataatatc aaccagattg ctcgaagtgt
     2281 taatcaatct cagttaattt ctggtgaaga attgcaggag ttgaaaaaag gaattggtga
     2341 attgataaaa gaagttgata aggaatttaa tctgcaagcg cagaagctaa aggagttcca
     2401 tggtcatcac taaacacttt gccattcacg gaaagagtta ccgcagaaag cttatcaagt
     2461 acattctcaa tcctgagaaa accaataatc ttgccttggt gtcggactat ggcatgaaga
     2521 attttctgga ctttcctagc tatgaggaaa tggtgcagat gtatcatgaa aatttcatca
     2581 gcaacgatac gctttacgat tttcgccacg acaggatgga agaaaatcaa cgaaaaatac
     2641 acgctcacca catcattcag tctttctcgc cagaggatca tctcactcct gaacaaatca
     2701 atcggatagg ttatgagact gtgaaggaat taactggtgg caaatttcgt tttatcgttg
     2761 cgacccatgt tgataaagac cacctgcaca atcacatcat tatcaattca gtagatagca
     2821 attctgacaa aaagctcaag tgggactaca aggtggagcg aaatcttcgc atgatttctg
     2881 accgtttttc taaaatcgca ggtgctaaaa tcattgagaa ccgctattct caccagcggt
     2941 atgaagtcta tcgtaagact aatcacaagt atgaactcaa gcagcgactc tattttttga
     3001 tggaacattc tagggacttt gaggatttca aaaagaatgc tccgctacta catgtggaga
     3061 tggatttccg tcacaagcat gccacctttt ttattacgga ctcaactatg aaacaggtgg
     3121 tgcgtggcaa gcaactcaat cgcaagcagc cttacacaga agaatttttt aagaactact
     3181 ttgccaaaag agaaatagaa agtctcatgg aatttttatt gctgaaagtt gagaatatgg
     3241 atgatttact tcagaaagca aaactttttg gactaactat caatcctaaa caaaagcatg
     3301 tttcttttca atttgcagga gtggaggtaa aggagacaga gctagaccag aaaaatcttt
     3361 atgatgtaga gtttttccaa gattatttta aaaatagaaa agattggcaa gctccagaaa
     3421 ctgaggattt cgttcaactt tatcaagaag aaaagttatc caaagaaaaa gaacttccaa
     3481 gcgatgagaa gttctgggag tcctatcaag agttcaagag taacagagat gccgttcatg
     3541 aatttgaggt ggagttgtca ctcaatcaaa ttgaaaaagt agtggatgat ggaatttacg
     3601 tcaaggtcaa gtttggtatt cgtcaggagg gacttatctt tgtgccgaac atgcagcttg
     3661 atatggaaga ggataaggtg aaggttttca tcagggaaac cagctcctac tatgtctacc
     3721 acaaagacgc tgccgagaaa aattgttata tgaaaggtcg aaccttaatt agacagttca
     3781 gctatgaaaa tcaaaccatt ccattacgca gaaaagcgac agtcgatatg attaaagaga
     3841 agattgcgga agtggatgct ttgattgaac tggaagtaga aaatcaatct tatgtcacga
     3901 ttaaagatga gttagtgcat gaactagcag cgtctgaatt gagaatcaat gagttgcaag
     3961 aacgaatgtc aaccttgaat caagtagcag aatatctact ggcttcagtt gaaagtaagc
     4021 aagaaatgaa attaaatctt tcaaaactga atataactga gaatatcagt gctaatattg
     4081 ttgagaaaaa attgaagagc ctggggaatc aactggaatt ggaaaggggc aggtatgaaa
     4141 agatggtagt caggctagat aaatttataa atcgcttaaa tacaggactt tcaaaaggag
     4201 atgggataga ttttcaaaaa taggaatcta gccaataatt atatacaatg tgtatctttt
     4261 gtaaaatata tttgaaattt agatttaatc atgttacaat taacattgaa atcaaataga
     4321 aaaggaagat gcaaacgtgg gcacactatt agcaacaaga ttaaaaaata gacgaaaaga
     4381 attaaaaatg tctcagcgag aattagctga gggtatctgt aaacaggggc aaattagtcg
     4441 attagagagt ggagagttca cccctggagc ggatttttta catgcccttg ctaagaaatt
     4501 aaaagttagt atggactact tttttgatga gcaggttgta gaggaagtca atgagttatc
     4561 agagtttaag aagttagcac agacatttat cacaaatcgt aattatgaat ctttaaaata
     4621 tatatatgaa ttggaaaatg taaaagccca tcgcttatct ctagcagata aattttacat
     4681 ggaatggata aagtctctta tagattttta tttttatgga cagaaagaag aggcaattac
     4741 acgattagag aatatgctac ctcagttaaa tgtatctgat ttgaactacc ttcaagtttc
     4801 aaacactcta ttcaattttt attatgatat tgaagattta gaacgtttta atgagattag
     4861 agacaagcta gagcatcaag tgaatcagct taatttaaac acaattgaag agttagaact
     4921 atcaattaag ttcaattata acgtttgtcg atacctatgg ttacagaaaa atattgaaga
     4981 agctattacg aaaatcacag ctacgataaa gcaatgtaag gagtacagga caacttatct
     5041 attagctgat ttgtatttat tgatgggaag tgtaagtgaa aatttttctt ctaaaagttc
     5101 agtaaaagaa tattttgaga ctgctcattt tctctacaaa cttgaggaaa acatgtcaat
     5161 ggctttgaaa gtcgaacatt attttgcaga tataacagaa taaggaatac ggtgttctag
     5221 tgagcatcgt tttttaaatc taatttgaat acaaaaaagt attaaataaa aaaaaataaa
     5281 aaaagattat aaaaatactt gacaagctaa ttttgaagtg ataaaataaa tgcaagaaaa
     5341 cgaaaggaga ggtatatgag aagtttgttt aggaaaattg ttgctttgtt agtcatagga
     5401 ttaatcctat tagggactgc tggaggaact caagttcata agatggctag agggattgat
     5461 ccaggtcctg cgaatggtat ttatagatag gagattat
//
LOCUS       DAVKNL010000091         5272 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVKNL010000091 DAVKNL010000000
VERSION     DAVKNL010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5272
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.091"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(411..1139)
                     /locus_tag="VW411_001985"
     CDS             complement(411..1139)
                     /locus_tag="VW411_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9794639.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            1297..2706
                     /locus_tag="VW411_001986"
     CDS             1297..2706
                     /locus_tag="VW411_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU9794640.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLELDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWLERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            2724..4019
                     /locus_tag="VW411_001987"
     CDS             2724..4019
                     /locus_tag="VW411_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9794641.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTEFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            4024..4332
                     /locus_tag="VW411_001988"
     CDS             4024..4332
                     /locus_tag="VW411_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9794642.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            4329..4637
                     /locus_tag="VW411_001989"
     CDS             4329..4637
                     /locus_tag="VW411_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9794643.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1785 a          881 c          956 g         1650 t
ORIGIN      
        1 tatgatttag aatttattta atatattaaa agaagatatg aacaaataaa ttgggagatt
       61 gaaattcatt tctagcaatt tgttagaagt cacagtctac tattctaacc acagtatact
      121 atatgtgtta caagaaaaga ctcaaaaact tctcgtcagt gtaagaatgt ttgaaacact
      181 atttaaaact aaataactat aagaagtaga tcaggtgaac aatttcagta cctactctat
      241 aagctatacg aagatatttt tcaacatgat ggatacaacg atcactcttt taaagccatc
      301 ttatttagaa aagagaaacc atttaagcat agatgggacc ttcttgccct gttttaaatt
      361 tttcttaaac tagagtctgt aaaagattgg cttcattttc acattttctc ctaggaggaa
      421 tgtcgtaatg caaaagaatt aaattcaaat aaatcatagc gatgacgact aatcgagtac
      481 tcaaacggag ttccgttatc caaataagcc acttgttcta cttcaaatac cggttccgtt
      541 ggaaggagct gcaagtaatg ttgctcattt tctgaactag aagaagctcg taagattttt
      601 gtagcactgg caatatgcaa tcctaacttt ccttcaatgt aattgtaaat cgatttttgt
      661 aaaatatctt cagtaatacc tggaataaca tcggtactca tataagtttg ttccagaaca
      721 taaggtttac cgtcaataat acgcaggcgg taaatattat aaactggact ctgcaaagcg
      781 acctgtagtt tttctgctaa aaattcattt gcaaattcta atttgaagtg aataatcctc
      841 gagtcaattg tagaatgagc atcttcagat atttttgtca gtccccggat atctctttct
      901 ggaacgatta attttctttt tgagctgcca cgagagagaa caaaggttcc ctgcccacgt
      961 tttctaaaaa ttaagccctc agaaactaac aagtctaaag cttttttgat cgtcattcga
     1021 ctacaggcaa actgtttagc taatgtcatt tcatcaggca attgttcgtt catggtatat
     1081 ttattacaaa gaatcttttc acgaatctca ttgtaaataa ataaatattt tgtactcatt
     1141 taatctggtt ctctctataa tattctaata gtagtataac aaagttcaag aaatgaggca
     1201 aacacaagat atgtatcaac aatttttaaa aatgtctaga caaattttaa aattatgcta
     1261 tactttatgt atagaataat agaaaggagg ttgagcatga aaaaaaagtt agtatttcct
     1321 aatctgtttt ggtggggagc tgcttctagc ggacctcaga cagaaggtca atatggaaaa
     1381 gtacatgaaa atgtgatgga ctactggttc aaaacgcatc cagaagattt tttcgataat
     1441 gtcggacctc ttgtagccag taactttttt catacttaca ccgaagattt ccacttgatg
     1501 aaggaaattg gagttaattc tttccgcact tccatccaat ggagtcgact catcaagaat
     1561 ttagagacag gtgagcctga tccaaaaggt attgctttct acaatgccat cattgaagaa
     1621 gctaaaaaga accagatgga tcttgtgatg aatttacatc attttgattt accagtggaa
     1681 cttcttcaaa aatacggtgg ttgggaaagc aaacatgtag tggagttatt cgtgaagttt
     1741 gccaagactg ctttcacatg ctttggagat aaggttcatt actggacaac tttcaatgag
     1801 ccaatggtca ttccagaagc aggatactta tatgctttcc attatccaaa tctaaaagga
     1861 aagggaaaag aggccgtaca agtcatctat aatctaaacc ttgctagtgc aaaagtgatt
     1921 caactatatc gctcattaga acttgatgga aagattggga ttattttaaa cttgacacct
     1981 gcttatccaa gaagtaattc tccagaagac ttagaagcaa gtcgatttac agatgacttc
     2041 tttaacaaag tcttcttgaa tccagctgtt aaaggaactt tcccagaaag attggtaaaa
     2101 cagctagaga gagatggcgt gttatggagt cataccgaaa aagagcttca actgatgaaa
     2161 tcaaatacgg ttgattttct tggagtaaac tactaccatc caaaacgtgt tcaagcacaa
     2221 gcaaatcctg aggaatatca gacgccctgg atgccagacc aatacttcaa agagtatgaa
     2281 tggctggagc gtcgcatgaa tccatatcgt ggttgggaaa tttttccgaa agccatttat
     2341 gatattgcta tgattgtgaa ggaagaatat ggtaatatcc catggtttat cagtgaaaac
     2401 ggaatgggtg ttgaaaacga agcacggttt atcgatgaaa atggagttat cgatgacgtg
     2461 tatcgtattg aattttatga agaacattta agatggctac ataaagccat tgaagaggga
     2521 agtcactgtt ttggatacca cgcttggacc gcatttgatt gctggtcttg gaataatgca
     2581 tataagaatc gttacggatt tatctccgtt gatttagaaa cgcaaaagag aaccatcaag
     2641 agctcaggaa gatggtatcg caaagtaagt gacaataacg gttttgaagt agaaattgag
     2701 gagtaaagag aggagatatt catatgaaaa actcaaaatt tatagaccaa tttgccacct
     2761 ttgctggtaa actagggaac caaattcatt taaaaaccct aagagatgca ttcgtaacag
     2821 taatgccatt atatattttg gcaggtttaa tcgttctttt gaacaacacg gtatttaagt
     2881 ggattttcca aggggataca ttaacaagat tccaatattg gggaataaca attgcaaacg
     2941 gtactttaag tatttcaggt atgattattg ctgtaatggt tggttatttc ttagctaaaa
     3001 acagagattt cgaaaacccg ttagcagcat caatgctatc attagtttct ttaattgtga
     3061 tgatgccaaa tacagtttct gtagttcctg acggagcaaa agatgcggta aacatttcag
     3121 gtgttctttc attcaacaac acaggtacag gcgcaatgtt cgccggggtt atcgtagcga
     3181 ttattgcaac agaattattc attgaattat caaacgttaa agctttacaa atgaaccttg
     3241 gtgaaaatat tccaccagct gttagtagat catttagcgt attacttcca gtcatgaccg
     3301 tcatctcctt atttggggtt gtttcagcat tattattcaa tataactgga atgaacttaa
     3361 tctcaatcat tacaatcttt attcaagaac caattcgtca tattggtaca agcttaatcg
     3421 gggtcattat tatttactct ttaggaaata tgttatggct atttggtatt caccaagcag
     3481 ttatttacag tgccatccta gaaccattac tattaattaa cattactgaa aacatcactg
     3541 cagcaaataa tggacaagcc attccacaca tcatcaacct atcacaaata caaacattcg
     3601 ctttaatggg tggtagtgga tctacattat gtttattaat agcaacattc ttagtgagtc
     3661 gcaatgctgt ctctaaaaac gtggctaaat tatcttttgg acctggtatc ttcaatatca
     3721 atgaaccagt attattcggt tacccaatcg tttataacat ttcattagct attccattta
     3781 tcacagttcc agtccttggt attttaatca gctacttagc aacagttaca gaattcatga
     3841 gtcctgcatt tatacaagtt ccttggacta caccagtatt cttaaatgca tggttagcaa
     3901 cagcagggga cgtgagagca gttctagttc aattcatcat ctttgcactt ggagttcttc
     3961 tatacattcc atttatcaaa gttaatgaca aagttgttga acaagaaatg gaaggttaag
     4021 attatgaaac aaattttatt agtatgtaat gcgggaatgt cgacaagtat gcttgttaaa
     4081 aaaatgcaac aaagtgcgac tgaacgtggg attgaaatct ctatccaagc aaaatcaatg
     4141 acagaagcta aaaagaatat ccatgaagca gatgtcattc taattgggcc acaaattcgt
     4201 tatgagttac ttgctgtgaa agaaattgca ggaaatatcc ctgtagatac cattgatatg
     4261 cgggattacg gaatgatgaa tggagcaaaa gttctggaac aagcacttgc atggatagga
     4321 gaaataagat gacagaagaa atggaatata tctgttttca gctcattgcc aatagtggag
     4381 cagccaaatc atcctttatt gaagccatcc aactagctaa ggcaggaaat ttaaaagaag
     4441 cgaaaattaa agttgaagaa gcggaagact ccttagtaga agcgcacaag attcactcta
     4501 atctcataca aaaagaagct actggagaaa aaataggatt ctccttgcta tttatgcatg
     4561 cagaagacca aatggcttca acagagatta ttcagttgct atctaaagaa ttcatcgatc
     4621 tctatcaaaa taaataggaa cactgtcatt cccatctgtc tttattaatg acgactacat
     4681 tgtaggaacc tccaaaagtt ggacaaaatg attgaaagga ttggatcctg cattacacaa
     4741 gatccaatcc ttttctttta ctctagtttt tataatatgt tgaatctaga atagtccact
     4801 gtagtttcta aaaaattgct ggaaatggat gttaagctcc aatttatttg tttatatctt
     4861 atttcagttt actatactag cttattattc tagtgaaaaa ttgaatgagc cactatttat
     4921 ttaatatgta tgatgaggga atgactcaaa aacttcttgt cgatataaga atatctgaga
     4981 tagtatttta aaacgaaagg actataaaag ctcatttcct tcgcacttat tttatagaga
     5041 tctatcatta tactctttag aaatcaaaaa gcaacacacc ttgtcatcgt tttatcgtaa
     5101 atctgttaat gagggggcta gtcttatctg caatctcaaa tcagtgtttt gagctgaatg
     5161 gcactcgttt tctagtttgc tctttgattt taattgggcc ttagtaaata ctactcttaa
     5221 attaaactat agtgaattga aataagatgt gaacaactct atcagaaaag ta
//
LOCUS       DAVKNL010000092         5196 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVKNL010000092 DAVKNL010000000
VERSION     DAVKNL010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.092"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            299..517
                     /locus_tag="VW411_001990"
     CDS             299..517
                     /locus_tag="VW411_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9794644.1"
                     /translation="MALNYKPLWIQLAKKGLKKTDVIAMAGLTTNVMAQMGKDKPITF
                     KNLERICKALSCTPNDIISFEDNFSDEE"
     gene            910..1935
                     /locus_tag="VW411_001991"
     CDS             910..1935
                     /locus_tag="VW411_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU9794645.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLG
                     NACKNAK"
     gene            1925..2932
                     /locus_tag="VW411_001992"
     CDS             1925..2932
                     /locus_tag="VW411_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9794646.1"
                     /translation="MRNKKQINLGIIFIICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     N"
     gene            2932..3939
                     /locus_tag="VW411_001993"
     CDS             2932..3939
                     /locus_tag="VW411_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9794647.1"
                     /translation="MQNLIIGIQKRKNRITLFSSLFLLIIISLSFFILLIGDESYSFS
                     TLIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQTMLRNPLASPDIIG
                     VTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKDGFSAARLIILGIG
                     FQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLIVSIIATILVLFFN
                     KRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPIACISFLAGPIALN
                     IGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSPYLIYLLIKMNRRN
                     I"
     gene            3939..4733
                     /locus_tag="VW411_001994"
     CDS             3939..4733
                     /locus_tag="VW411_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9794648.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVNS"
BASE COUNT         1947 a          729 c          873 g         1647 t
ORIGIN      
        1 gtaagcagca caccaatgaa tttaaagatt ctttaaagag tcttattttg tgatgaaaat
       61 ttaatatgta aatctcagac gatagaaatt aaaaactcta tcgtcttttt tatactcaaa
      121 attaggaggt aaaaatggta aggataagag gtcccactta aaacaattta tggcaaaata
      181 aggacggaat aacacaacaa attctctaaa acaaatcact aaatcaatgt aagattgaat
      241 gaaatcaata tttatgctat aattaaataa atttaatgaa gaaaaaaaga gggatattat
      301 ggcacttaac tataaaccat tatggataca gttagcaaaa aaaggactaa agaaaacaga
      361 tgtaatagct atggcaggac ttacaacaaa tgttatggca caaatgggaa aggataaacc
      421 aattacattt aagaatttag aaagaatatg taaggcttta tcttgcactc ctaatgatat
      481 tattagtttt gaagataatt ttagtgacga ggaatagaaa atgactttaa ggacagaaga
      541 tcaagttagg gattatgcaa gagaagtata ggctttaatg aagttgaaga aaacatcaat
      601 caaggtactg gtcaaataac tacttttaat caattaggct tcaagggata ttcaaataag
      661 ccagatggtt ggtatttacc taaaaatatg aatgatgtag caataatcct tgaaacaaaa
      721 tcagaagaaa gagatattag caaacaaatt tttattgatg agttaatgaa aaatatagac
      781 ataatttaac taaaaataaa aactagatcc ttttttgaaa aaattatatt attaaatttg
      841 taactgtatc tattgacaat gataattatt atcgatacaa tagacttgaa atatgtttaa
      901 ggagttttta tgaaaaacaa attttttcta atagctattt tagctatgtg tatagttttt
      961 agcgcttgtt cttctaattc tgttaaaaat gaagaaaata cttctaaaga gcatgcgcct
     1021 gataaaatag ttttagatca tgctttcggt caaactatat tagataaaaa acctgaaaga
     1081 gttgcaacta ttgcttgggg aaatcatgat gtagcattag ctttaggaat agttcctgtt
     1141 ggattttcaa aagcaaatta cggtgtaagt gctgataaag gagttttacc atggacagaa
     1201 gaaaaaatca aagaactaaa tggtaaagct aacctatttg acgatttgga tggacttaac
     1261 tttgaagcaa tatcaaattc taaaccagat gttatcttag caggttattc tggtataact
     1321 aaagaagatt atgacactct atcaaaaatt gctcctgtag cagcatacaa atctaaacct
     1381 tggcaaactt tatggagaga tatgattaaa attgattcaa aagccttagg tatggaaaaa
     1441 gaaggtgatg agttaatcaa aaatactgaa gctcgtatat ccaaagaatt agaaaaacat
     1501 ccagaaatca aaggaaaaat caaaggaaaa aaagtattat ttactatgat taatgctgca
     1561 gatacatcaa aattctggat ttatactagc aaagatccaa gagcaaatta tttaacagat
     1621 ttaggtctag ttttccctga atcattaaaa gaatttgaga gtgaagatag ttttgcaaag
     1681 gaaatttctg cagaagaagc aaataagata aatgatgctg atgtaatcat aacttatggt
     1741 gatgataaaa ctcttgaagc tttacaaaaa gatcctcttt taggtaaaat aaatgcaatt
     1801 aaaaatggtg ccgttgctgt aattccagat aatacaccgt tagcagcctc atgcactcca
     1861 acaccacttt caataaacta cactattgaa gaatacctaa atcttttagg aaatgcatgc
     1921 aaaaatgcga aataaaaaac aaataaacct aggcataatt tttataatct gcctaggtct
     1981 tcttattaca atatttttgt cattaaagct tggaacaaaa gaaattaata tcagagattt
     2041 tttagcagct tttggaatgg gtaatacaaa tgatgatttt attaaatcaa ttatatataa
     2101 aagaatacct agaactattt ttgcaatttt agcaggttct agtcttgcca taagcggtgt
     2161 attgatgcaa tcagttacta gaaacccaat agctgatcca ggtatactcg gtataaacac
     2221 aggagcaagt cttagtgtag taattggtct ttctttttta ggaatttcat caagcataag
     2281 ccatataagt tttgcaatca ttggtggctt agtaagtgca atttttgtat acgcgattgc
     2341 tgtaagcgga aaagcaggcc ttacccctat aaaacttgcc ttatcaggaa cttgtgttag
     2401 tatggcttta agcagttttg taagtttttt aattttaccg aataataacg tcttagacaa
     2461 atttagattt tggcaaatag gtagccttgg agcagctaca ttatcttcta tatctacact
     2521 actacctttt ataattttag gtcacttgat agctatattt atttcatcag atttaaacgc
     2581 tttagctatg ggtgatgaaa tggctgttgg tcttggagtt aatgttaata ggataagatc
     2641 acttgcaata attgcaagtg tgcttttatg ttcaagtatt actgcaattg gtggacctat
     2701 tggcttcgta ggtcttatag ttcctcactt ttgtggctta tttataagca aagatatacg
     2761 cacaatgacc atttcttcat cttttatagg tgcagagctc ttgcttatat gtgatataat
     2821 cggccgtatg ttaggtaaac caggtgaaat tgaagtaggg ataattactg caataatcgg
     2881 gggtccagta cttatttatg taactatgaa aaatagaggg gttaataact aatgcaaaat
     2941 ttaattatag gtattcaaaa aagaaaaaat agaataacac tattttcctc actattttta
     3001 ttaataataa tcagtctatc atttttcatt ttacttatcg gagatgaaag ttattctttt
     3061 tcaactttga ttaaagtctt aaatagtgaa actgttcctg gagctagttt ttcgattatg
     3121 gaaattagat taccaaaatt attagcagga attatagctg gctggtcttt tggattggca
     3181 ggatttatct ttcaaactat gttaagaaat cctcttgcaa gtcctgatat aatcggtgtc
     3241 acaagttctt catctattgc agcggtcttt tgcatattgg tattaaaaac aaatagttta
     3301 actactggaa ttatttcaat aacttgtgga ctaacatcat ctttaatatt atttttacta
     3361 gctaaaaaag atggtttttc agcagcaaga ctgataatat taggtattgg ttttcaagct
     3421 gtcacaagag caggcacctc atttttattg ttgaaagtag caagatatga attacaagaa
     3481 gttatgagat ggctcagtgg ctctttatct tttacaaagt tagatgacat acctcttgtt
     3541 ctaatagtaa gtattattgc tactatatta gttttatttt ttaataaaag actagaaatt
     3601 attgaacttg gtgaagaaat agcaatcgga cttggagcaa atcccgagct ttcaaggctt
     3661 gttttaattt tttgcgctgt atctttaact gctttttcta cttcaattac aggaccaata
     3721 gcttgtatat cttttttagc tggtcccata gccttaaata ttggcaagaa aagaagccca
     3781 atattagctg gattggttgg aattttacta gttttgttat cagacatatt ctctcaaaat
     3841 attttaccag ctagatatcc agtaggtgtt gtaactggct tgttaggttc accatactta
     3901 atatacttac taataaaaat gaacaggagg aatatataat gaaaggtctg tggtcaaata
     3961 atcttacctg cggttatgat gaaaaaataa tcttggaaaa tataaatata aaaatacctg
     4021 aagaaaaaat atcagttatt attgggtcaa atggttgtgg gaaatcaaca ctcattaaaa
     4081 ccttgtctcg acttataaag ccattagagg gagaagtatt gcttgataat aaatcaatta
     4141 attcttataa agaaaaagat ttagcaaaac acatagctat attacctcaa tctccaataa
     4201 tccctgaatc aataacagta gctgatcttg taagccgtgg tcgtttcccc tacagaaagc
     4261 cttttaagag tcttggaaaa gatgaccttg aaataataaa cagatcaatg gttaaggcca
     4321 atgttgaaga tctagcaaat aacctagttg aagaactttc tgggggtcaa aggcaaagag
     4381 tatggatagc tctagcccta gcccaagata caagtatcct acttttagat gagccaacta
     4441 cttacttgga tatctcatat caaatagaac tattagacct cttgactgat ctaaaccaaa
     4501 aatataagac aaccatttgc atgattttgc acgatataaa tctaacagca agatacgctg
     4561 attacctatt tgcaattaaa gaaggtaaac ttgttgcaga gggaaagcct gaagatatac
     4621 taaatgataa actagttaaa gatatcttta atcttgaagc aaaaattata cgtgacccta
     4681 tttccaattc gcctctaatg attcctattg gcaagcacca tgttaactct taataattga
     4741 atattggaat tcatttaaaa gtcgcaaatt ttgaactgac cctcaaaagt taggccaaaa
     4801 aactaactaa aggaggtcag ttttttaatg gcaaatacag cacagaattt aagataaaat
     4861 tagtaaaaga atatcatgaa tgaaaaatag gatatagaga ataacagcaa tattaaaaag
     4921 atcaggactg ataataaaca aaaagaaagt acaacgacta gttcaaaagc taaaacttca
     4981 agtaaaaagt tattcaagaa aatctagaaa atactcatcc tacaaaggac aggtaggaaa
     5041 aatatcagac aacaaaataa aaagaaatat ttattaccga aaaaaagggg aaatcgagtt
     5101 aagtaccaac aggctcatct attccaaaaa gatatttact cgatggaaaa actcctagaa
     5161 ttagtgctac aaatattgac aatggtatct taggtt
//
LOCUS       DAVKNL010000093         5087 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVKNL010000093 DAVKNL010000000
VERSION     DAVKNL010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.093"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            <1..521
                     /locus_tag="VW411_001995"
     CDS             <1..521
                     /locus_tag="VW411_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9794649.1"
                     /translation="YCKEKNNISFKATFGDIKWSLLNKQAHRNTVSGDFGCLSNTRKA
                     QGRHLEQEGNIKHALIYYIESLIITISGLENNFSATDYPVYYPDSIPDYSLKHIQTLM
                     ESLSDDDYDFAFDEALFRFSILNANHFLSKEDIDYLRVNLPRSTAEEINNYLKKYECY
                     SPLNNLELDDFE"
     gene            complement(688..759)
                     /locus_tag="VW411_001996"
     tRNA            complement(688..759)
                     /locus_tag="VW411_001996"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(724..726),aa:Arg,seq:cct)
     gene            complement(802..1149)
                     /gene="rplS"
                     /locus_tag="VW411_001997"
     CDS             complement(802..1149)
                     /gene="rplS"
                     /locus_tag="VW411_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU9794650.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(1268..1597)
                     /gene="crcB"
                     /locus_tag="VW411_001998"
     CDS             complement(1268..1597)
                     /gene="crcB"
                     /locus_tag="VW411_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU9794651.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(1591..1965)
                     /gene="crcB"
                     /locus_tag="VW411_001999"
     CDS             complement(1591..1965)
                     /gene="crcB"
                     /locus_tag="VW411_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU9794652.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIYLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYLSLI
                     LYLLLSIGGGLLLAYYLGRKKW"
     gene            complement(1962..2228)
                     /locus_tag="VW411_002000"
     CDS             complement(1962..2228)
                     /locus_tag="VW411_002000"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEU9794653.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            complement(2344..2787)
                     /locus_tag="VW411_002001"
     CDS             complement(2344..2787)
                     /locus_tag="VW411_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEU9794654.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELAAKVG"
     gene            2891..3826
                     /locus_tag="VW411_002002"
     CDS             2891..3826
                     /locus_tag="VW411_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEU9794655.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQKILKQYNITDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKVHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            3880..4164
                     /locus_tag="VW411_002003"
     CDS             3880..4164
                     /locus_tag="VW411_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEU9794656.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(4221..4439)
                     /locus_tag="VW411_002004"
     CDS             complement(4221..4439)
                     /locus_tag="VW411_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794657.1"
                     /translation="MSISPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVVRDNS
                     IAHYGRVETAISFLPSSMEIEPTDESGT"
     gene            complement(4436..5010)
                     /locus_tag="VW411_002005"
                     /pseudo
     CDS             complement(4436..5010)
                     /locus_tag="VW411_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1612 a         1073 c          882 g         1520 t
ORIGIN      
        1 attattgtaa agaaaaaaac aatatctctt ttaaagcaac ttttggtgat ataaaatgga
       61 gtctcttaaa taaacaagct cataggaata ctgtatcagg agattttgga tgcttatcta
      121 acacacgaaa ggctcaggga agacatttgg aacaagaagg taatattaaa catgctttaa
      181 tatattacat agaatctttg ataattacta tttcaggatt agaaaacaat ttttcagcca
      241 ctgattatcc agtatattat cccgattcga tacctgacta ctcactaaaa catattcaaa
      301 cattaatgga atcattatct gatgacgatt atgattttgc ttttgatgaa gcattatttc
      361 gcttctcaat tttgaatgca aatcattttt tatctaagga agatattgac tatttaagag
      421 ttaatttacc tcgttccact gctgaagaaa taaacaatta cttaaagaaa tatgaatgtt
      481 atagtccttt aaataattta gaacttgacg attttgaata aattgactat acaaacattt
      541 atatactcga tatagtctca attttatctg atgattgccc aaatttttca ataataaaac
      601 gcataatatt atggagacaa tcccctatat tatgcgttct tttaatatca aagacttttt
      661 gacaaacttc tttgatatct aattacatgc cccctgcagg aatcgaacct gcaactactc
      721 cttaggaggg agttgttata tccattgaac taagggagct agataaaaac tctgctaaat
      781 gagcagagtt ttttagtcga attaacgacg gatttctttg atacgagctg ctttaccttg
      841 aagagcacgc aagtagtaca atttcgcacg acgtacttta ccgtaacgaa caacttcgat
      901 tttttcaaca cgtggagtgt ggattgggaa gatacgctca acacctacac cgttagagat
      961 tttacgaact gtgtagtttt ctgagattcc agcaccttta cgtgcgataa caacaccttc
     1021 aaaaatctgg atacgttcac ggttaccttc gacaactttc gcatgtacac gaacagtgtc
     1081 accaggacgg aatgatggga tatctgtacg aagttgacct tcagtcaagc tttggattaa
     1141 tggattcatt ttattctcct atctttgtca atcttgagga atcttcctca gcggataaac
     1201 tgtatttttg tgcgtccatt acacacaaga tatagtttac caaatttcta aaaaaaagta
     1261 aagaaattta tagcaaaatt cctaaaaaaa tcgcaaccaa accacctatg taagtcaaag
     1321 tcaggtaaga ataaaagacc ttcttatcac ttagcagtct ttgaagttcg tcattcaagg
     1381 tcgaaaaagt tgttaaaccg ccacaaaatc ctgttgctag aatagcatat acttccttag
     1441 actctacatg attgtagaat actccaatta aaaaacaacc taaaagatta gctatgagcg
     1501 ttccgagtgg caatttagaa gcttgattat agcgagaaaa gaaataccgt acgagtgctc
     1561 ccaagccaca ggcgattgca agatagacga ttaccatttc ttcctcccca aatagtaagc
     1621 taaaagcagg cctccaccga tactcaaaag caaatacagt atcaaactaa gataacgccc
     1681 tgtatcaagc agcttcacag tatcaagcat taggctggaa aaagttgtta aacctccgca
     1741 aaaacccgtc cccagtgcta aaatcaagcc cttactagtc cccttataga ccagatagcc
     1801 cttgacaaga taaatcaagc agaaaattcc cagatagttg acaaagagag tgccccaagg
     1861 gaaatctgga ctggctggta accaggtgga aactagatat cggacaagtc cgcccaacat
     1921 agcagctaga aaaattccta gcggataaaa ttgttctttt ttcatttgat attttgatcc
     1981 tgataatcac gcgaacgttt gagtatgtcc gaaaaagtcg cgacaatagt ctcctgatag
     2041 cgcttgtcct ctacacgact cctaaccttt tcaaaaataa cttcttctct cttagtatct
     2101 aaaatcggtt tacctgaagc tttcttataa gcaaccaccc cctcaactaa atgcattcgt
     2161 tcttctaaca gtttgacaat ttggtcgtcg atttgatcga tttcttgacg aataatatct
     2221 aaatccatac agtctcctcc tttatttgaa ttattgtatc aaaaagccat caattaggct
     2281 agtaaaatcc caactctcga taagaaaaat gcactgattg attacatcag cgcactttta
     2341 tgtttatcct actttagcag ccaattcttc tgcgaattgt tccaagcgtt caatatcttc
     2401 ttcctcggca gaaagatcaa ctttaacaca ctctgaacct ttttctgctc ctgttgacac
     2461 aaagacacgg tcaaagtcat caacagcctt acagaattca tcgtagaagg tatctcctga
     2521 gccgaccact ccgtagattt tgccattcaa gttgagatct gctaggtctt cgtagaagtc
     2581 catcatctca tctggcaatt caccatcacc ataagtatag gtcgcaacga tagcgatgtc
     2641 tgcttccaag aagtctgaag cgtcaacagt tgtacattca tcaacatcaa catccaagcc
     2701 caagtcacgt aatttgtctg ctacaatatc tgcaatttct tcggtattac cggtcatact
     2761 ggcaaataca atttttgcta atgccatatc gtcctcctca atttatcttt ctccattata
     2821 tcacatcttt ttcattttaa aaataaaaat tcttgtgcta caatgaaatg agaaagaatt
     2881 gaggttattt atggagattt gccaacaaat tttagaaaaa attaaagaat acgacaccat
     2941 tatcattcat cgtcatatga aaccagaccc tgatgccttg ggaagtcagg tgggattgaa
     3001 agccttgctg gaacatcatt tcccagaaaa aaccatcaaa gccgtcggtt ttgatgaacc
     3061 aactcttact tggatggctg agatggatct tgttgaagat agagcctacc aaggcgcact
     3121 tgtcatcgtc tgtgatacag ctaatactgc tcgtatcgat gataagcgct atagtcaagg
     3181 tgattttctc attaagattg accaccatcc aaatgatgat gtatacggtg acctgtcttg
     3241 ggtcgatact agttcaagta gcgctagcga gatgattacc ctatttgccc aaacaaccca
     3301 actagccttg gcagatcgcg atgctgagtt gctctttgca ggaattgtcg gtgatacagg
     3361 tcgcttcctc tacccttcta ccactgcacg gactcttcgc ctggctgctt atttgagaga
     3421 acataacttt gactttgcgg ctctcactcg caaaatggac actatgagct acaaaattgc
     3481 taaactgcaa ggctacatct acgaccatct ggaagtggat gaaaatggtg ctgctcgcgt
     3541 tatcctgagt cagaaaatct tgaaacaata caatataacc gatgctgaaa ctgcggccat
     3601 tgtaggtgca cctggacgca ttgacagagt gagtctctgg ggaatttttg tcgaacaggc
     3661 tgatggccac taccgagttc gcttacgcag taaagtccat cctatcaatg aaattgccaa
     3721 ggagcatgat ggtggaggcc accctctagc aagtggtgct aattcctata gcctagaaga
     3781 aaacgaaatc atctaccaaa agttaaaaaa cttgcttaaa aactgataaa atacttgcca
     3841 aacttttcag aatctgatag actagtatag taacaatcta tggctcgcaa agagaccatg
     3901 gcagaaagga aatattgcaa aatgaaaaaa gatatccatc cagaatatcg cccagttgtc
     3961 ttcatggaca caactactgg ttaccaattc cttagcggtt caacaaaacg ctctaacgaa
     4021 acagttgagt tcgaaggcga aacttaccca ttgatccgtg tggaaatttc atcagactca
     4081 cacccattct acactggacg tcaaaagttc actcaagcag atggacgcgt ggatcgtttc
     4141 aacaaaaaat acggtctcaa ataatgataa gagaacagtt ttggctgttc ttttttgttt
     4201 cttgaaatca actgctgttt tcatgttcca gactcatctg taggttcgat ttccatgcta
     4261 ctaggcagga aggaaatagc tgtttcaaca cgtccataat gagctatact attgtcacga
     4321 accacacttt cattgatggt ccaagtggaa ttcattttct taaaagcttc tcggactttt
     4381 tccaaatctt tggaggcaat ggcctgctct aaggtttcaa aacgaggact tatactcatc
     4441 tgctttcaaa aagcattcta gtccatctcc gattaccgat ggactttatc acctccttct
     4501 ccagtccttg tatgacatct tgaagttgat tcatgacatc ttccaaagtt cgaaaggctt
     4561 tattcttaaa tccacgttta cgaatctctt tccacacttg ttcaatgggt tcatctctgg
     4621 tgtgtatgga ggaataaagg taaaatcaat attagtcgga atatttaagg tacttgattt
     4681 atgccatata gcattgtcca taacgagtaa aaggataagc ttgtgaaagc tcttctaaaa
     4741 aggcgttcat ccacactcct ttttataaac ctgaaataag gcatcaattg taacaaattc
     4801 tcctgcctct gtagccttca aatgacgggc aagaaaggct ttctcttcct caactgtcat
     4861 atatgcatgg ttacgaccac cacgtgtttc ttgaaggaga gagtcgagtc cgaactcctc
     4921 atattttttt acgtttcgcc aaatcgttgt ttgattacag tctaaaagct ctataatctc
     4981 tttataagat ttgcccatca gacgaaatat agtagattga aactagaata gtacacctct
     5041 acttctaaaa cattgttaga aatcgatttg tcctgttctt gtttcat
//
LOCUS       DAVKNL010000094         5031 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVKNL010000094 DAVKNL010000000
VERSION     DAVKNL010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5031
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.094"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(<1..217)
                     /locus_tag="VW411_002006"
     CDS             complement(<1..217)
                     /locus_tag="VW411_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEU9794658.1"
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGGAARGLQ
                     LGIKTGTWQGAATGAAGGAILGGVAYAA"
     gene            complement(286..555)
                     /gene="blpJ"
                     /locus_tag="VW411_002007"
     CDS             complement(286..555)
                     /gene="blpJ"
                     /locus_tag="VW411_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HEU9794659.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLAGAFVGAHIGAIGGGLTCLGGMVGDKLGLSW"
     gene            complement(1022..1219)
                     /gene="blpI"
                     /locus_tag="VW411_002008"
     CDS             complement(1022..1219)
                     /gene="blpI"
                     /locus_tag="VW411_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="HEU9794660.1"
                     /translation="MNTKMMEQFSVMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLICVGSGIMSSL"
     gene            1501..2088
                     /locus_tag="VW411_002009"
     CDS             1501..2088
                     /locus_tag="VW411_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEU9794661.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            2105..2995
                     /locus_tag="VW411_002010"
     CDS             2105..2995
                     /locus_tag="VW411_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin ABC transporter"
                     /protein_id="HEU9794662.1"
                     /translation="MKSTLGIISVGLVITYILQQVMSFSRDYLLTVLSQRLSIDVILS
                     YIRHIFELPMSFFATRRTGEIISRFTDANSIIDALASTILSLFLDVSILILVGGVLLA
                     QNPNLFLLSLISIPIYMFIIFSFMKPFEKMNHDVMQSNSMVSSAIIEDINGIETIKSL
                     TSEENRYQNIDSEFVDYLEKSFKLSKYSILQTSLKQGTKLVLNILILWFGAQLVMSSK
                     ISIGQLITFNTLFSYFTTPMENIINLQTKLQSAKVANNRLNEVYLVESEFQVQENPVH
                     SHFLMGDIEFDDLSYKYGFG"
     gene            3011..3658
                     /locus_tag="VW411_002011"
     CDS             3011..3658
                     /locus_tag="VW411_002011"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9794663.1"
                     /translation="MNLTIKQGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINHQ
                     DIKNIDKKVLRRHINYLPQQAYIFNGSILENLTLGGNHMISQEDILKACEVAEIRQDI
                     ERMPMGYQTQLSDGAGLSGGQKQRIALARALLTKSPVLILDEATSGLDVLTEKKVIDN
                     LMSLTDKTILFVAHRLSIAERTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            3669..5030
                     /locus_tag="VW411_002012"
     CDS             3669..5030
                     /locus_tag="VW411_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEU9794664.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLSNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LVASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTSPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
BASE COUNT         1656 a         1036 c          750 g         1589 t
ORIGIN      
        1 ttgctgcata ggctacacct ccaagtatag ctccacccgc agcaccagtt gctgcacctt
       61 gccatgttcc tgttttaatg cctagttgaa gacctcttgc tgctcctcct ccaacacctg
      121 ctttggcaaa atctccccaa ttgcatccgc caccttcaac gcaagcaagc atttcagtat
      181 ccataacaga aaattgtgac atcatttttg tatccatgac aaatactcct tttttaaaaa
      241 actaaaataa atcagaatag aatcctcata attttactat aagtcttacc aacttagtcc
      301 caatttatca ccaaccatac ctcctaagca tgttaatcca cccccaattg caccaatgtg
      361 tgctccaaca aatgcaccag caagtccagc tactcctaaa gtggccaaac ctgctccagt
      421 tccaccagtt ataattcccg tagtgactcc tgtaatcagt gcattttgac aatcagtgga
      481 gctatacccc ccttcaactt tcgcaagcat ttcagtatcc ataacctcta actgtgacaa
      541 catttttgta ttcatgatga atacctcctt tttattttca atttgttacc aaagtcttaa
      601 attcaataaa caaatagatt ttttatagta tctttttgat tttcttaaaa aagtatatac
      661 gtctactatc ttcttaaagg tagcagtacc tattttttag tctaagattt caataatctt
      721 gagtatctaa aatatcttaa tttcgttatt ctccttgcaa taaaaagttt tactatacta
      781 tttattaact tgcagaaagc aaaaaatatt agtaaataat agtttatagt taagtttttt
      841 attcctacca atccatcaac taagtaaagc atcaacgatt acataaacga ttgataatat
      901 aattaaaatt ttgctaacta tcttattctc atcattctta gataactttg atattttgta
      961 agtaagtaaa taagacagta aattaatagc gataataata ctatatttaa gaatcataat
     1021 cttacaaaga ggacataatt cctgaaccta cacaaataag tgttgctgct cccccagtta
     1081 tcggaccagt cgcagcagct aatagtactg ctccaataca accaccgatt gcagatccta
     1141 aattgcctct tcctccacta actatttcga gttcttcatt atccataaca gaaaattgtt
     1201 ccatcatttt tgtattcatg acaaatactc cttttttctt tttttatttt tgtcttgttg
     1261 taactttgat aagtttagta tatcatcgtt ttttaaaatt tttcatccag atcttgaatt
     1321 gtcatcgaaa cgtcttgaat tagctttttt atttcaagcc acctctaaat gtttaaaaaa
     1381 aataatttct aatcactttt ttaccattca ggaagtttta atgactattc aagatttcat
     1441 aaaatatgaa cttagtttta tgacataata gacctatcca ctatatgaaa ggaattgcca
     1501 atgacttctt ataaacgtac atttgttcct caaatagatg cgagagactg tggtgtcgct
     1561 gccttagcct cgattgctaa attctatggt tcagattttt ctctagctca cttgagagaa
     1621 cttgcaaaga ccaataaaga agggacgact gctcttggca ttgtaaaagc cgctgatgaa
     1681 atgggctttg aaacaagacc tgttcaagca gataaaacgc tctttgacat gagtgatgtc
     1741 ccctatccat ttatcgttca cgttaacaaa gaaggaaaac tccaacatta ctatgttgtc
     1801 tatcaaacaa agaaagacta tctgattatt ggtgatcctg acccttctgt aaaaatcact
     1861 aaaatgtcaa aagaacgctt tttctatgaa tggactggag tagctatttt tctagctacc
     1921 aaacccagct atcaacccca taaagataaa aagaatggtc tactaagcaa gcttccttcc
     1981 tctgattttc aaacaaaaat ctctcattgc ttacattgtt ctctcaagct tattggtcac
     2041 tattatcaat ataggtggtt cttactatct ccaaggaatc ttggatgaat acattccaaa
     2101 tcagatgaaa tcaactttag gaatcatctc agttggtctg gttatcacct atatcctcca
     2161 acaagtcatg agcttctcca gagattatct cctaaccgtt ctgagtcaga gattaagtat
     2221 tgatgtgatt ttatcctata ttcgccatat ttttgaactt cccatgtctt tctttgcgac
     2281 acgtcgtaca ggagaaatca tttcacgatt cacagatgct aactctatta tagatgcctt
     2341 ggcttctacc attctttctc tttttctgga tgtttctatt ctgattcttg taggaggcgt
     2401 cttactggca caaaacccta atctcttcct tctttctctt atttccattc ctatatacat
     2461 gttcatcatc ttttctttta tgaaaccttt cgaaaaaatg aaccatgatg tcatgcaaag
     2521 taattctatg gttagctctg ccattatcga agatatcaac gggattgaaa ctataaagtc
     2581 gctcacgagt gaagaaaatc gctatcaaaa tatagacagc gaatttgtag attatttgga
     2641 aaaatccttt aagctcagta aatattctat tttacaaacg agtttaaagc agggaacaaa
     2701 attagttctg aatatcctta tcctatggtt tggcgctcaa ttagtcatgt caagtaaaat
     2761 ttctatcggt cagctgatta cctttaacac acttttttct tactttacaa ctcctatgga
     2821 aaatattatc aacctccaaa ccaaactcca atctgcgaag gtcgctaata accgtttgaa
     2881 cgaagtctat ctagtcgaat ctgaatttca agttcaagaa aaccctgttc attcacattt
     2941 tttgatgggc gatattgaat ttgatgacct ttcttataag tatggttttg gatgagatac
     3001 cttaacagat attaatctca cgattaaaca aggagataag gttagcctag ttggagttag
     3061 tggttctggt aaaacaactt tagccaaaat gattgtcaat ttctttgaac cctacaaagg
     3121 gcatatttcc atcaatcatc aggatattaa aaacattgat aaaaaagtct tgcgccgtca
     3181 tattaattac ctaccccaac aagcctatat ctttaatggc tctattttgg aaaacttaac
     3241 cttgggcggt aatcatatga ttagtcaaga agatattcta aaagcttgtg aagtagctga
     3301 aatccgtcaa gacattgaaa gaatgcctat gggctatcaa actcagctct ctgatggagc
     3361 tggtctatca ggaggacaga agcaacgaat cgctctcgct cgtgctcttt taactaaatc
     3421 tcctgtttta atactagatg aagctactag cggtcttgat gtcttgactg agaaaaaggt
     3481 tatagataat cttatgtctc taactgataa aaccattctc tttgtagccc atcgtctcag
     3541 tatagccgaa cgaaccaacc gtgtcattgt tcttgaccag gggaaaatca ttgaagttgg
     3601 tagtcaccaa gagttaatgc aggcgcaagg tttctaccat catctattca ataaataagg
     3661 agaatgtcat gaatcctaat ctttttagaa gcgtcgagtt ttatcagaga cgttaccata
     3721 actatgcgac agtgttaatt atacctcttt cattactatt tactttcatc ttgattttct
     3781 cccttgttgc cacaaaagaa attactgtta cttcccaagg agaaatcgcc cctacaagtg
     3841 tcattgcctc cattcagtca accagtgata atcctatcct agctaatcat ttagtggcaa
     3901 atcaagtagt tgaaaaaggg gacttactca tcaaatactc tgaaacaatg gaagaaagtc
     3961 agaaaactgc cttagcaact caattacaaa gacttgagaa gcaaaaagaa ggacttggaa
     4021 ttttgaaaca aagcttagaa aaagcgactg atcttttttc tggcgaggat gaatttggct
     4081 accataatac ctttatgaat tttactaaac aatcccatga tattgaactg ggtatcacaa
     4141 agactaacac cgaagtttca aatcaagcta atctttccaa tagcagttca tcagctattg
     4201 aacaagaaat tacaaaagtt caacaacaaa ttggagaata tcaagagttg agagatgcta
     4261 tcataaataa cagagcacgc ttaccaactg gcaatccgca ccagtcaatt ttgaatcgtt
     4321 atcttgtagc ctcacaagga caaacacaag gaactgcaga ggagccattt ttatctcaaa
     4381 ttaatcaaag tattgcaggt cttgaatcat ctatcgcaag cctcaaaatt cagcaagctg
     4441 gtatcggaag tgtagcaact tatgataaca gtttagcaac caaaattgaa gtactccgca
     4501 ctcagttttt acagacagcc tcacagcaac aactaactgt ggagaatcaa ttaacagaat
     4561 taaaagtaca actagatcaa gccacacagc gtttggaaaa caatacctta acctccccaa
     4621 gtaaaggtat cgttcatctg aacagcgaat ttgaaggtaa aaatagaatt ccaactggta
     4681 cagaaattgc tcaaatattc cctgtcatca cagatacaag agaagtacta atcacttact
     4741 acgtatcttc tgactatcta cctctactag ataaaggaca aactgtaaga ttaaaactgg
     4801 agaagattgg aaatcacggc accaccatca tcggccaact tcagacaatt gatcaaactc
     4861 ctaccagaac agagcaagga aatctcttta aattaaccgc tcttgcaaaa ctatctaacg
     4921 aggatagtaa actcatccaa tatggcttac aaggtcgcgt cactagtgta actacaaaga
     4981 aaacatattt tgattatttc aaagataaaa ttttaacaca ttctgattaa t
//
LOCUS       DAVKNL010000095         5013 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVKNL010000095 DAVKNL010000000
VERSION     DAVKNL010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.095"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(<1..1781)
                     /locus_tag="VW411_002013"
     CDS             complement(<1..1781)
                     /locus_tag="VW411_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec-dependent serine-rich glycoprotein
                     adhesin"
                     /protein_id="HEU9794665.1"
                     /translation="MFKVLRGGVDTTQVMTETVEDKVSHSITGLDILKGIVAAGAVIS
                     GTVATQTKVFTNESAVLEKTVEKTDALATNDTVVLGTISTSNSASSTSLSASESASTS
                     ASESASTSASTSASTSASESASTSASTSISASSTVVGSQTAAATEATAKKVEEDRKKP
                     ASDYVASVTNVNLQSYAKRRKRSVDSIEQLLASIKNAAVFSGNTIVNGAPAINASLNI
                     AKSETKVYTGEGVDSVYRVPIYYKLKVTNDGSKLTFTYTVTYVNPKTNDLGNISSMRP
                     GYSIYNSGTSTQTMLTLGSDLGKPSGVKNYITDKNGRQVLSYNTSTMTTQGSGYTWGN
                     GAQMNGFFAKKGYGLTSSWTVPITGTDTSFTFTPYAARTDRIGINYFNGGGKVVESST
                     TSQSLSQSKSLSVSASQSASASASTSASASASTSASASASTSASESASTSASASASTS
                     ASASASTSASASASTSASASASTSASASASTSASASASTSASESASTSASASASTSAS
                     ESASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASTSASESAS
                     TSASASASTSASASASTSASASASTSAS"
     gene            complement(1779..1892)
                     /locus_tag="VW411_002014"
                     /pseudo
     CDS             complement(1779..1892)
                     /locus_tag="VW411_002014"
                     /inference="COORDINATES: protein motif:HMM:TIGR03715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KxYKxGKxW signal peptide domain-containing
                     protein"
     gene            complement(2139..2600)
                     /locus_tag="VW411_002015"
     CDS             complement(2139..2600)
                     /locus_tag="VW411_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9794666.1"
                     /translation="MGKERRKQGNLERKKRVVKKRKIPLEELKAFVEAHPDAFLREIA
                     ARFDCALPSVWAVLKQIKVILKKTTSFREQKPEKVSEFLDILDNLKDLPVLYIDETGI
                     DRYLYRPYAGAPRGEKVYGKISGRRFERTNEVEQKLNGSFLIRYIDSQIRE"
     gene            complement(2567..2668)
                     /locus_tag="VW411_002016"
     CDS             complement(2567..2668)
                     /locus_tag="VW411_002016"
                     /inference="COORDINATES: protein motif:HMM:NF013842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794667.1"
                     /translation="MKGGHRHVEAAKVFDVGVRTLFTWEKKDVNKGT"
     gene            2977..3357
                     /locus_tag="VW411_002017"
     CDS             2977..3357
                     /locus_tag="VW411_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9794668.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            3456..3677
                     /locus_tag="VW411_002018"
     CDS             3456..3677
                     /locus_tag="VW411_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU9794669.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            3694..4008
                     /gene="trxA"
                     /locus_tag="VW411_002019"
     CDS             3694..4008
                     /gene="trxA"
                     /locus_tag="VW411_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU9794670.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            4314..4847
                     /gene="queF"
                     /locus_tag="VW411_002020"
     CDS             4314..4847
                     /gene="queF"
                     /locus_tag="VW411_002020"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEU9794671.1"
                     /translation="MKRQLALVVFSGGQDSTTCLFWVMQHYETVEAVTFAYGQRHHLE
                     IQITREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
     gene            4859..4936
                     /locus_tag="VW411_002021"
                     /pseudo
     CDS             4859..4936
                     /locus_tag="VW411_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
BASE COUNT         1467 a         1055 c         1008 g         1483 t
ORIGIN      
        1 gatgcactgg ttgatgctga agccgatgca ctcgttgatg ctgaagctga tgcactagta
       61 cttgctgagg ctgacgcact cgttgatgcc gattcagatg cactggttga ggccgacgca
      121 cttgtcgacg ctgaggctga cgcactggta cttgctgaag ccgacgcact tgtcgacgct
      181 gaggctgacg cactggtact tgctgaagct gaggcgcttg tcgatgctga ggctgacgca
      241 ctggttgatg cagattcaga agctgatgtg cttgctgagg ctgacgcact cgttgatgct
      301 gactcagacg cactcgttga cgctgaagcc gatgcacttg ttgacgctga ggctgaggcg
      361 ctagtacttg ctgaagcgga cgcactcgtt gatgctgaag ccgatgcact tgttgacgct
      421 gaggctgagg cgctagtact tgctgaagcg gacgcactcg ttgatgccga ttcagacgca
      481 ctcgttgacg ctgaagccga ggcacttgtt gacgctgagg ctgaggcgct agtacttgct
      541 gaagctgagg cgctttgact agcacttact gagagtgact tagactgtga aagtgactga
      601 ctggtcgtgc tagattcaac tacctttcct ccaccattga agtagttaat tccaattcta
      661 tctgttctag cagcgtaagg ggtaaatgta aaggatgtat ccgttccagt aattggtaca
      721 gtccaagatg atgttaatcc atatcctttc ttagcaaaga aaccattcat ttgggcacca
      781 tttccccaag tatacccact accctgcgtc gtcattgtag atgtattata ggatagaacc
      841 tgtctaccat ttttgtcagt aatgtagttc tttacacctg aaggtttacc aagatcactg
      901 ccaagggtta acattgtttg tgttgaagta cctgaattat agatagaata tccaggacgc
      961 atacttgata tattaccaag atcatttgtt ttaggattca catacgtaac cgtataggta
     1021 aaggtcaatt ttgaaccatc atttgtcact ttcaatttat agtaaattgg aacacgatat
     1081 accgaatcta caccttcacc tgtataaact tttgtctcac ttttagcaat gtttagactt
     1141 gcattaattg caggggcgcc atttacaatc gtattgccag aaaaaacagc agcatttttt
     1201 atagaagcca gcaattgctc gatggaatcc actgaacgct tgcgtcgctt agcataagat
     1261 tggagattga catttgtaac tgatgctaca taatcactag ctggtttctt acgatcttct
     1321 tcgaccttct tagcagttgc ttctgtagcg gcagctgttt gtgaacctac cacagtagat
     1381 gatgcagaaa tacttgtcga agccgatgta cttgctgatt ctgatgcact tgtacttgct
     1441 gaggtcgaag cgctggttga ggctgactca gatgcagatg tacttgccga ctctgaagct
     1501 gacaaactag ttgaactcgc tgaattactt gtagatatcg tacctagaac tactgtatca
     1561 tttgttgcca aagcatccgt tttctctaca gttttttcaa gtactgctga ctcatttgta
     1621 aataccttcg tttgagttgc aacggttcca cttatgacag ctcccgcagc aactatcccc
     1681 ttgaggatat caagcccagt aattgaatga cttactttat cttctaccgt ttcggtcatg
     1741 acctgagtag tatcaacacc acctcgcaag accttaaaca agccaaaaag agaagtcgag
     1801 gcccgtagcc aatgcttccc tgatttgatt aatttgaacc gagttactcg gtctgtttct
     1861 ctatattcac ccttctgacg tctaaaaaat attttgaaat ttcctttcga gtattatttt
     1921 catgtatgga tataaataaa ttaccatact ataattatag aatagggaac ccaaaatagc
     1981 aagaaacttg aggtattttt gctaaaaaaa aaaaagcatt ttactatttt atatatatat
     2041 atatatatta taagataagt tttttataaa gacacgcaaa tgagtataaa tcagctaatt
     2101 tttggtttat attcctattt ttatgcataa ttattctttc attctctaat ttgtgaatca
     2161 atatatctga ttagaaaact accattgagt ttttgctcca cctcattagt ccgctcaaaa
     2221 cgccgtccgc taatcttgcc atagactttc tcccctctag gagcccctgc ataaggacga
     2281 tagaggtagc ggtcgattcc cgtttcgtca atatatagga ctggtaaatc ttttaggtta
     2341 tccaaaatat caagaaactc agaaactttc tcaggctttt gttccctaaa actggtcgtc
     2401 ttttttaaaa tgaccttaat ctgctttaaa actgcccata cggagggcaa agcacaatca
     2461 aaacgggccg caatttcccg taaaaaagcg tctggatgag cctctacaaa ggctttcaat
     2521 tcttctaaag ggatcttacg ctttttgacg actcgctttt tccgctctaa gttcccttgt
     2581 ttacgtcttt cttttcccac gtgaagagag ttctgacgcc gacatcaaaa actttggccg
     2641 cctcgacatg tctgtgccct cctttaatgg aatctaatgc tccttgttta aaatcagtac
     2701 tatatgcata gctttattag accatgctta ttttaaacta agcaactata gatagagaaa
     2761 tgaaataaaa tctgaattcg aatgtgataa ggagttagaa attgaaacaa taggattccc
     2821 ccattattca cacaaaaata aaaccactaa aaaacagagc ttgccagcat tcttgaaaag
     2881 tagtataatt attgcatgcg caatcatctt gtgacgcaga gactgcccgt taatggactt
     2941 tcttctattt atgactctaa aaagaaagga gacactatga cctatttgga aaaatggttt
     3001 gacttcaatc ggcgtcagaa agaaattgaa agtctcttgg aagagaccat tgcccaacag
     3061 agcgagcaaa gtctgacctt gaaagagttc tacctgctct actatctgga cttggctgaa
     3121 gaaaaatctc tacgccagat tgacctgcca gataagcttc atctgagccc gagcgcagtt
     3181 tctaggatgg tggctcgctt ggaagctaaa aattgcggtc tactcagtcg catgtgttgc
     3241 catcaagata gacgctctag ttttatctgc ctgacaaatg atgggcaaaa gacactggcc
     3301 tcgctacaaa agactgtcga agaaagcttg gaagcaggtt tggatttctt gatttaagat
     3361 gattttaaaa aaagttgcgt gcgcaatcat ttttcttgac attctctttt ataaggagta
     3421 aaataaagtt agcattaaac aaaggagttt taaacatgat tgaaattacc tatatagatg
     3481 ccagcaagaa cgaaagaact gtgactttcg agtcttatga agactttgaa cgttcgcaac
     3541 aagcttgcct tatcggcgtc gcagactact accctgtcca aaaattaact tacaagggtc
     3601 ataatttgga ctaccatggg acttatggag atatcttctt ctatctcatg aaacaagatt
     3661 taagccaata taactaaaaa aaggagaaat acaatggcaa aagcaattac agatgcaaca
     3721 ttcgaacaag aaacaaaaga cggtttggtc ttagtagact tctgggcaac ttggtgtggt
     3781 ccatgtcgta tgcaaggtcc aatcttggac aaattgtctg aagaactttc agaagatgtc
     3841 ttgaaaatcg ttaaaatgga cgttgatgaa aatccaaaca cagctcgtgc ttttggaatc
     3901 atgtctattc caactcttct cttcaaaaaa gacggccaag ttgtcaaaca agttgcaggt
     3961 gttcacacag cagaacaaat caaggccatc attgctgaat tgagctaatc aaacgagaga
     4021 ccaagtactt tctttggtct cttttttctt gccctttgcc atttttcaaa aaatatgcta
     4081 gactatagta gattgaaact agaatagtac acctctactt ctaaaacatt gttagaaatc
     4141 gatttgactg tcctgatcga tttgtcctgt tcttatttca ttttactata taagtagaat
     4201 attacgatgt ttgggatatg ccatcgctaa aaaaacctcg atttggtatt ttttagctcc
     4261 ctaatgggag ctttttgcgt gctctgaaca tttcccattt tggaaggagt actatgaaac
     4321 gtcaattagc cttggtcgtc tttagtggtg gtcaagattc aacaacctgc cttttctggg
     4381 tcatgcaaca ctatgaaaca gtcgaagctg tcacctttgc ctacggccaa cgtcatcacc
     4441 tcgaaattca aattactaga gaaatcgcta aggaacaggg cattcgtcac catatcctcg
     4501 atatgtctct gctgggacaa atcactgctc agccagactt tgcgacgatt catatttcct
     4561 acattcctga caagctctgt gtcgagtcaa aatccctcaa actatatcta tttagctacc
     4621 gaaaccacgg agatttccac gaaaactgta tcaacaccat cgggaaagac ttggtcaact
     4681 tgctagaccc tcgctattta gaagtctggg gaaaattcac tccgcgcggt ggcatttcaa
     4741 tcgaccccta ctacaactac ggtaagcaag gaactaagta tgagggcttg gcagaacaac
     4801 gcctcttcca acacgacctt tatccagaga aaattgacaa ccgctaaagt catactcaat
     4861 gaaaatcaaa gagcaaacta ggaagttagc cgcaggttgc tcaaagcact gttttgaggt
     4921 tgtggataga actgacgaag tcagatcaaa acactgtttt gaggttgtgg atagaactga
     4981 cgaagtcaga tcaaaacact gttttgaggt tgt
//
LOCUS       DAVKNL010000096         4919 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVKNL010000096 DAVKNL010000000
VERSION     DAVKNL010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.096"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            52..1597
                     /locus_tag="VW411_002022"
     rRNA            52..1597
                     /locus_tag="VW411_002022"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1649..1721
                     /locus_tag="VW411_002023"
     tRNA            1649..1721
                     /locus_tag="VW411_002023"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1682..1684,aa:Ala,seq:tgc)
     gene            1846..4747
                     /locus_tag="VW411_002024"
     rRNA            1846..4747
                     /locus_tag="VW411_002024"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            4824..>4919
                     /gene="rrf"
                     /locus_tag="VW411_002025"
     rRNA            4824..>4919
                     /gene="rrf"
                     /locus_tag="VW411_002025"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1375 a         1006 c         1451 g         1087 t
ORIGIN      
        1 gaaaaaaaca ataaatctgt cagtgacaga aatgagtaag aactcaaact ttttaatgag
       61 agtttgatcc tggctcagga cgaacgctgg cggcgtgcct aatacatgca agtagaacgc
      121 tgaaggagga gcttgcttct ctggatgagt tgcgaacggg tgagtaacgc gtaggtaacc
      181 tgcctggtag cgggggataa ctattggaaa cgatagctaa taccgcataa gagtagatgt
      241 tgcatgacat ttgcttaaaa ggtgcacttg catcactacc agatggacct gcgttgtatt
      301 agctagttgg tggggtaacg gctcaccaag gcgacgatac atagccgacc tgagagggtg
      361 atcggccaca ctgggactga gacacggccc agactcctac gggaggcagc agtagggaat
      421 cttcggcaat ggacggaagt ctgaccgagc aacgccgcgt gagtgaagaa ggttttcgga
      481 tcgtaaagct ctgttgtaag agaagaacga gtgtgagagt ggaaagttca cactgtgacg
      541 gtatcttacc agaaagggac ggctaactac gtgccagcag ccgcggtaat acgtaggtcc
      601 cgagcgttgt ccggatttat tgggcgtaaa gcgagcgcag gcggttagat aagtctgaag
      661 ttaaaggctg tggcttaacc atagtaggct ttggaaactg tttaacttga gtgcaagagg
      721 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccggtgg
      781 cgaaagcggc tctctggctt gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      841 gattagatac cctggtagtc cacgctgtaa acgatgagtg ctaggtgtta gaccctttcc
      901 ggggtttagt gccgtagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      961 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
     1021 agcaacgcga agaaccttac caggtcttga catccctctg accgctctag agatagagct
     1081 ttccttcggg acagaggtga caggtggtgc atggttgtcg tcagctcgtg tcgtgagatg
     1141 ttgggttaag tcccgcaacg agcgcaaccc ctattgttag ttgccatcat ttagttgggc
     1201 actctagcga gactgccggt aataaaccgg aggaaggtgg ggatgacgtc aaatcatcat
     1261 gccccttatg acctgggcta cacacgtgct acaatggctg gtacaacgag tcgcaagccg
     1321 gtgacggcaa gctaatctct taaagccagt ctcagttcgg attgtaggct gcaactcgcc
     1381 tacatgaagt cggaatcgct agtaatcgcg gatcagcacg ccgcggtgaa tacgttcccg
     1441 ggccttgtac acaccgcccg tcacaccacg agagtttgta acacccgaag tcggtgaggt
     1501 aaccgtaagg agccagccgc ctaaggtggg atagatgatt ggggtgaagt cgtaacaagg
     1561 tagccgtatc ggaaggtgcg gctggatcac ctcctttcta aggataagga actgcgcatt
     1621 ggtcttgttt agtcttgaga ggtcttgtgg ggccttagct cagctgggag agcgcctgct
     1681 ttgcacgcag gaggtcagcg gttcgatccc gctaggctcc attggtgaga gatcaccaag
     1741 taatgcacat tgaaaattga atatctatat caaatagtaa caagaaaata aaccgaaaac
     1801 gctgtagtat taataagagt ttatgactga aaggtcagaa aataaggtta agttaataag
     1861 ggcgcacggt ggatgccttg gcactaggag ccgacgaagg acgtgacaaa cgacgatatg
     1921 ccttgggtag ctgtaagtaa gcgatgatcc agggatttcc gaatggggga acccaacagg
     1981 taatacctgt tacccacatc tgttaaggat gtgaggagga agacgcagtg aactgaaaca
     2041 tctaagtagc tgcaggaaga gaaagcaaaa gcgattgcct tagtagcggc gagcgaaacg
     2101 gcagaagggc aaaccgaaga gtttactctt cggggttgta ggactgcaat gtggactcaa
     2161 agattataga agaatgattt gggaagatca gccaaagaga gtaatagcct cgtatttaaa
     2221 atagtctttg tacttagcag tatcctgagt acggcgggac acgtgaaatc ccgtcggaat
     2281 ctgggaggac catctcccaa ccctaaatac tccctagtga ccgatagtga accagtaccg
     2341 tgagggaaag gtgaaaagca ccccgggagg ggagtgaaat agaacctgaa accgtgtgcc
     2401 tacaacaagt tcgagcccgt taatgggtga gagcgtgcct tttgtagaat gaaccggcga
     2461 gttacgttat gatgcgaggt taagttgaag agacggagcc gtagggaaac cgagtctgaa
     2521 tagggcgcct tagtatcatg acgtagaccc gaaaccatgt gacctaccca tgagcaggtt
     2581 gaaggtgcgg taagacgcac tggaggaccg aaccagggca cgttgaaaag tgcttggatg
     2641 acttgtgggt agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata
     2701 gctttagggc tagcgtcgac attagagatt cttggaggta gagcactgtt tgggtgaggg
     2761 gtccatcccg gattaccaat ctcagataaa ctccgaatgc caatgaatta tggtcggcag
     2821 tcagactgcg agtgctaaga tccgtagtcg aaagggaaac agcccagacc accagctaag
     2881 gtcccaaaat aattgttaag tggaaaagga tgtggggttg cacagacaac taggatgtta
     2941 gcttagaagc agctattcat tcaaagagtg cgtaatagct cactagtcga gtgaccctgc
     3001 gccgaaaatg taccggggct aaaacaattt accgaagctg tggatacctt tataggtatg
     3061 gtaggagagc gttctatgtg tgatgaaggt ataccgtgag gagtgctgga acgcatagaa
     3121 gtgagaatgc cggtatgagt agcgaaagac aggtgagaat cctgtccacc gtaagactaa
     3181 ggtttccagg ggaaggctcg tccgccctgg gttagtcggg acctaaggag agaccgaaag
     3241 gtgtatccga tggacaacag gttgatattc ctgtactaga gtatgtagtg atggagggac
     3301 gcagtaggct aactaaagca gacgattgga agagtctgtc taagcagtga ggtgtgaatt
     3361 gagtcaaatg cttaattcta taacattgag ctgtgatggg gagcgaagtt tagtagcgaa
     3421 gttagtgacg tcacactgcc aagaaaagct tctagcgttt aaacatactc tacccgtacc
     3481 gcaaaccgac acaggtagtc gaggcgagta gcctcaggtg agcgagagaa ctctcgttaa
     3541 ggaactcggc aaaatgaccc cgtaacttcg ggagaagggg tgctgactta aagtcagccg
     3601 cagtgaatag gcccaagcaa ctgtttatca aaaacacagc tctctgctaa atcgtaagat
     3661 gatgtatagg gggtgacgcc tgcccggtgc tggaaggtta agaggagtgc ttagcgtaag
     3721 cgaaggtatg aattgaagcc ccagtaaacg gcggccgtaa ctataacggt cctaaggtag
     3781 cgaaattcct tgtcgggtaa gttccgaccc gcacgaaagg cgtaatgatt tgggcactgt
     3841 ctcaacgaga gactcggtga aattttagta cctgtgaaga tgcaggttac ccgcgacagg
     3901 acggaaagac cccatggagc tttactgcag tttgatattg agtgtctgta ccacatgtac
     3961 aggataggta ggagtctaag agatcgggac gccagtttcg aaggagacgc tgttgggata
     4021 ctacccttgt gttatggcca ctctaaccca gataggtgat ccctatcgga gacagtgtct
     4081 gacgggcagt ttgactgggg cggtcgcctc ctaaaaggta acggaggcgc ccaaaggttc
     4141 cctcagaatg gttggaaatc attcgcagag tgtaaaggta taagggagct tgactgcgag
     4201 agctacaact cgagcaggga cgaaagtcgg gcttagtgat ccggtggttc cgtatggaag
     4261 ggccatcgct caacggataa aagctaccct ggggataaca ggcttatctc ccccaagagt
     4321 tcacatcgac ggggaggttt ggcacctcga tgtcggctcg tcgcatcctg gggctgtagt
     4381 cggtcccaag ggttgggctg ttcgcccatt aaagcggcac gcgagctggg ttcagaacgt
     4441 cgtgagacag ttcggtccct atccgtcgcg ggcgtaggaa atttgagagg atctgctcct
     4501 agtacgagag gaccagagtg gacttaccgc tggtgtacca gttgtcttgc caaaggcatc
     4561 gctgggtagc tatgtaggga agggataaac gctgaaagca tctaagtgtg aaacccacct
     4621 caagatgaga tttcccatga ttatatatca gtaagagccc tgagagatga tcaggtagat
     4681 aggttagaag tggaagtgtg gcgacacatg tagcggacta atactaatag ctcgaggact
     4741 tatccaaagt aactgagaat atgaaagcga acggttttct taaattgaat agatattcaa
     4801 ttttgagtag gtattactca gagttaagtg acgatagcct aggagataca cctgtaccca
     4861 tgccgaacac agaagttaag ccctagaacg ccggaagtag ttgggggttg ccccctgtg
//
LOCUS       DAVKNL010000097         4756 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVKNL010000097 DAVKNL010000000
VERSION     DAVKNL010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4756
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.097"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            54..1076
                     /gene="cbpC"
                     /locus_tag="VW411_002026"
     CDS             54..1076
                     /gene="cbpC"
                     /locus_tag="VW411_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HEU9794672.1"
                     /translation="MKLLKKMMQVALAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDNLFDNQPVNEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     EGHWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDASGKML
                     TDWQKVNGKWYYFGSSGSMATGWKYVRGKWYYLDNKNGDMKTGWQYLGNKWYYLRSSG
                     AMVTGWYQDGSTWYYLDPSNGDMKIGWTKVNGKWYYLNSNGAMVTGSQTIDGKVYNFA
                     SSGEWI"
     gene            1094..2092
                     /gene="cbpJ"
                     /locus_tag="VW411_002027"
     CDS             1094..2092
                     /gene="cbpJ"
                     /locus_tag="VW411_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpJ"
                     /protein_id="HEU9794673.1"
                     /translation="MKILKKTMQVGLTVFFFGLLGTSTVFADDSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDSLSGEMVVGWQYIPFPSKGSTIGPYPNGIRLEGFPKS
                     EWYYFDKNGVLQEFVGWKTLEIKTKDSVGRKYGEKREDSEDKEEKRYYTNYYFNQNHS
                     LETGWLYDQSNWYYLAKTEINGENYLGGERRAGWINDDSTWYYLDPTTGIMQTGWQYL
                     GNKWYYLRSSGAMATGWYQEGTTWYYLDHPNGDMKTGWQNLGNKWYYLRSSGAMATGW
                     YQDGSTWYYLNAGNGDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYNGEWVR"
     gene            complement(2210..3442)
                     /locus_tag="VW411_002028"
     CDS             complement(2210..3442)
                     /locus_tag="VW411_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9794674.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALWIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLLALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLTLITSILCQAYIVIFMTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(3485..4447)
                     /locus_tag="VW411_002029"
     CDS             complement(3485..4447)
                     /locus_tag="VW411_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEU9794675.1"
                     /translation="MTSSTEFGNFVSNLSFQHGIAGLLFPLNKLYPPELDSKILSIIK
                     KAVTIRTTHTYEYQYSLLFGDAGYLWLLLHLFSISKNQYYLQLANVTAKKLIENYDTL
                     EEIDFALGKSGVLLSLIKYYQFTNDNTLKIFIHNSIGEIYHYFLQRDTAKESILDYSF
                     AHGYCGIAYALFAYSKVLEPSMFYNDLHTFHTELKKLLEKVTSNTENLGNLQLSWCKG
                     ISGIILYLCMYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLM
                     KKIQQVILACSERDDHGLLMFQGDSGKADLFDFGIGSMGYIGVY"
BASE COUNT         1567 a          740 c         1016 g         1433 t
ORIGIN      
        1 atattgacat acaatctagt ttttactaca atcgttttag gaggatatat aaaatgaagc
       61 ttttgaaaaa aatgatgcaa gtcgcattag cagtcttttt ctttggtttg ctagctacaa
      121 atacggtatt tgcgaatacc acaggtggcc gatttgttga taaggataat agaaaatatt
      181 atgtaaaaga tgatcataaa gcaatctatt ggcataaaat agacggtaaa acttactatt
      241 ttggtgatat tggagagatg gttgtcggtt ggcaatactt agaaattcct ggaacaggtt
      301 atcgtgataa tttattcgat aaccaaccag ttaatgaaat tggccttcag gagaagtggt
      361 actattttgg acaagatggt gctttgctag aacaaacaga taaacaagta ctagaggcaa
      421 aaacgtctga aaatacagga aaagtatacg gtgaacaata tcctctatct gctgaaaaga
      481 gaacttatta ttttgataat aattatgctg taaagacagg ctggatttat gaagagggtc
      541 attggtatta tttaaataag ctaggaaatt ttggcgatga ttcttacaat ccactaccaa
      601 ttggtgaagt tgctaagggt tggactcaag attttcatgt tactattgac attgatagaa
      661 gcaaacctgc tccatggtac tacctagatg cttcaggtaa gatgcttaca gattggcaaa
      721 aagtaaacgg aaaatggtat tattttggct cctctggttc tatggcaaca ggttggaaat
      781 atgtacgagg caaatggtat tacttagata ataaaaatgg tgatatgaaa acaggatggc
      841 aataccttgg taacaagtgg tactacctcc gttcatcagg agctatggta actggctggt
      901 atcaagatgg ttcaacttgg tactatttag atccttctaa tggagatatg aaaataggtt
      961 ggacaaaagt aaatggaaaa tggtattatc tcaattcaaa tggagcaatg gttacaggta
     1021 gccaaactat cgatggtaaa gtttataatt tcgcctcatc tggtgagtgg atttaatatt
     1081 ggagggtata tagatgaaaa ttttgaaaaa aactatgcaa gttggactga cagtattttt
     1141 ctttggtttg ctagggacca gtacagtatt tgcagatgat tctgaaggat ggcagtttgt
     1201 ccaagaaaac ggaagaacct actacaaaaa gggggacctc aaagaaacct actggcgagt
     1261 gattgatggt aagtactatt attttgattc tctatctgga gagatggttg tcggctggca
     1321 atatatcccg tttccatcta aaggtagtac aattggtcct tacccaaatg gtatcagatt
     1381 agaaggtttt ccaaagtcag agtggtacta cttcgataaa aatggagtgc tacaagagtt
     1441 tgttggttgg aaaacattag agattaaaac taaagacagt gttggaagaa agtacgggga
     1501 aaaacgtgaa gattcagaag ataaagaaga gaagcgttat tatacgaact attactttaa
     1561 tcaaaatcat tctttagaga caggttggct ttatgatcag tctaactggt attatctagc
     1621 taagacggaa attaatggag aaaactacct tggtggtgaa agacgtgcgg ggtggataaa
     1681 cgatgattcg acttggtact acctagatcc aacaactggt attatgcaaa caggttggca
     1741 atatctaggt aataagtggt actacctccg ttcctcagga gcaatggcca ctggctggta
     1801 tcaggaaggt accacttggt attatttaga ccacccaaat ggcgatatga aaacaggttg
     1861 gcaaaacctt gggaacaaat ggtactatct ccgttcatca ggagctatgg caactggttg
     1921 gtatcaagat ggttcaactt ggtactacct aaatgcaggt aatggagaca tgaagacagg
     1981 ttggttccag gtcaatggca actggtacta tgcttatagc tcaggtgctt tggcagtgaa
     2041 tacgaccgta gatggctatt ctgtcaacta taatggcgaa tgggttcggt aatgacaggg
     2101 cgcttataat tatattagaa cactcgctta atcaaagaag tgactttgtt gtacttgacc
     2161 tgacgattgg ggagattaaa agcatgtgaa atctacacat gctttttcgt tagttatgag
     2221 tgttgaactt gactaaccat tgacaaaaga taagagcaat taaaataaga tacaaaaata
     2281 tcaatgctaa ctgcaccgaa acgcccgatg ctatggatag ggctaatagg gagagcaggg
     2341 acggggcgag aatgtccact gtgctcagag cagagcccac cgtccccatc ttgtcactgg
     2401 ggatatgggc aaagacagct gcttgtaggc gagggctgag aatgccgata atcgtagaac
     2461 tgatgaaact ggtcatgaaa attacgatat aggcttgaca aaggatacta gttattagag
     2521 tcaataggga aatctcacag aaaaccaaga ggtgcgtgaa acggatattt ttaaatagat
     2581 tactgctggt catattgcca aggatagctc cacaagacat gatgatggag agcagggcga
     2641 ggagttgccc aatgctcagt tgtgctagtg tctgttggat gaagcggagg gcaatcagcc
     2701 gaggggacac atccaagatg gcaatcatac tggtcgtcag aaacagtagt atgacggtat
     2761 cttttaacct caggagcaca gccagcgact gtttaagatg ttgaaaatat ttcttaaaac
     2821 tcagtgctgt atgtgacatt tcaattcttt tttcaacctc atacaaggta tgtcgaataa
     2881 catacaggcc caaaaaggca atgacaaaag tcagagtgtt gataagggaa atagtttgaa
     2941 tacttataac attgataagg aatgcgccag ccagattggc gacaatacgg acaaccctca
     3001 tcagagactg cctgaacccc atggcatcat gcaggtcgtc cttaattaca ctgatgtaga
     3061 gggcgttcat catgtaggca gacaggtagc tcaaggtgtc agagatgagg ttgatgatga
     3121 caatcataat gactgaaact aatgaaaagg gcgacaagac gagaaatact gtcaaaatag
     3181 cgtagagcag gattttgatt ttggcaatcc agagtgcgtg tttgactcta ttttcttgaa
     3241 aatctcctag aatcccgaga aagagttgcg atagtagggg aaccatctct gaaatggaaa
     3301 tcagtaaaat cgctagcgga gcgaaattgt actgagccac ataattgata atggcgaggt
     3361 agaaaatact gtcgccgaat gatgaaaaac agctgtttag cagcaactgt ctatatatgt
     3421 tgtttgtcca aaataatttc atacttttct ccttatgtct gcacatcgaa tgggaattta
     3481 ttatttaata gacaccaata taccccatgc ttcctattcc gaagtcaaac aaatctgctt
     3541 taccactatc tccttgaaac atcagtaaac cgtgatcatc tcgttcagaa catgctaaaa
     3601 ttacctgttg gatttttttc atcagtaatt tgttttgatt gtagacagtg gtttgtagta
     3661 agctagttat tccgtggcaa tatcctgtca tcatttttag atgatggtta aaaacaaatt
     3721 cttgatattt actaataata tctttgtttc cgtcacaatc gtacatacaa agatataaga
     3781 ttattccgga aattcctttg caccaagaaa gttgtaaatt tcctaaattt tcagtattag
     3841 aagtaacttt ttctaataat ttttttaatt cagtatggaa tgtatggaga tcattataaa
     3901 acatagaagg ttctaagact ttagaatagg caaataaagc atatgcaatt ccacaatatc
     3961 catgagcaaa gctatagtct aaaatgcttt ctttggctgt atctctttgt aggaaataat
     4021 gataaatttc ccctatacta ttgtggatga aaattttaag agtattgtca ttggtaaatt
     4081 gatagtattt tattaatgat aataggacac cagattttcc caatgcaaag tctatttcct
     4141 ctagagtatc ataattctct attaattttt tagcggtgac gtttgctaat tgtagatagt
     4201 attgattttt actgatagaa aataaatgta ggagtaacca tagatagcct gcatcaccaa
     4261 atagcagtga gtattgatat tcatatgtgt gtgtcgttct aattgtcact gccttcttga
     4321 tgatagagag tattttagaa tccagttctg gggggtacaa tttatttaga ggaaacagta
     4381 gtccagctat tccgtgttgg aatgataaat tacttacgaa atttccaaat tctgttgaag
     4441 aagtgatcct tccttcagaa tttacagttt tatctagtaa aaattgtaga acactattac
     4501 attctgtttt gtaatcatat ttatgttcct ctattttttt aattttgact aaattatttg
     4561 tttgacttaa atttagtaga cattgtaacc aaaatagttg ctcatctgtg agctgacctt
     4621 gagtatgtga tagttctagt aattgaatag gatcctttag ttagattcaa tcaatctata
     4681 acacaacaac ataataatac ttttaggata acttatcaaa tattatagta aaatgagata
     4741 ataacaggat aaatcg
//
LOCUS       DAVKNL010000098         4664 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVKNL010000098 DAVKNL010000000
VERSION     DAVKNL010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.098"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            89..2698
                     /locus_tag="VW411_002030"
     CDS             89..2698
                     /locus_tag="VW411_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEU9794676.1"
                     /translation="MDYNFNLEHPFFFTNNDYSTDTSIKYQVSLPFNWHEVMNNDEWV
                     YQYPIGKFVERQGWKIHISSEYNSSHELLQDVAKICHEMRIPFKHLSTEDKFIMRNGK
                     LVSRGFSGKFITCYPNQNELESVLQRLESALKQYNGPYILSDKRWDEAPIYLRYGVFR
                     PSRDDEKKVAIDELIVGDEVVKDERLPVFKIPKGIVPPDFLNKWLDKKDKKQGDFPFI
                     IDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLGFDGTYSSEKLASECKALKILN
                     EWSETPKIYWHGKIWEHTFLGIEHMKGVPLNRWVTNNFPLYEVVDKTKDYLLRVSKIV
                     EKLIDLTNKFHSENVYHQDLHLGNILVKDEDEISIIDWEQAVFSNDEKVVHKVAAPGF
                     RAWRETLPSEIDWYGIRQIAHYLYMPLVTTSDLTYNYVSQTRIEGKKLFESLGYTREH
                     IDYVESLLSYLDSKCPQIENISRKKVLKPMHEIRTIESEQDIQDFIIKLLRGFTLTYG
                     QWRKEFQSRFFPVHYYGLNFNQGIAFSDLAILWSYQQLAKKVKNFKFDDYYEIRTQVI
                     NEAVNNFKKSSLSGLFDGKIGTIWLIYEFGEIDRAVELFTTHFIEIFENSQNKNLYSG
                     QAGILLVGLYFLSKGGIDNKLGEEILIRLREYTLNYIENPETFCKVGASDVQSNDPYE
                     NFGGLLYGHAGVAWLFGEAYKLTGESIYKNGLELAVDKELVAYKVDSNNSLQYSQGHR
                     LLPYLATGSAGLLLLINRNKEILSSKYLKYLTSLERATDVVFCVLPGLFNGFCGLEVA
                     NNIYSDIDDNFSGQKKLIEQLYRYLCVIEEGFVIAGDNGLKITTDIASGFAGVAIGLV
                     SIMDNKLTILPQI"
     gene            2731..>4664
                     /locus_tag="VW411_002031"
     CDS             2731..>4664
                     /locus_tag="VW411_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU9794677.1"
                     /translation="MNQSNEIFLNTIQEKTHYKRSISSSFQELKKWNSLIFRRGRYWY
                     QFDNWSIPNTGVIKEWYVEDNDWIPKVSSSSLDNSNSLKIVFDVEKYTLPDDSILQDV
                     RISSDESYIVLVVSSLRSTNLIGIKKYTMEELFIIEDISVESSFYLGKFGVMYTRSKE
                     YGRPSKLFYKSFDSFTEEELFEENECSFRLKIVHIDSNNCFVKSVDFQKGRIFLYSFD
                     RTGFVRHSYTETVAPTPRDIALFSTEKAQYFLGLSSTEEKKDQLILRETSSGDRVSIT
                     IPYQDRARRVHCIGRYILLDCSNAANSVFYLATFKNNSLRELSVNKITFDEKTTLYEN
                     SFSDRVLLFLERRTFYEKILSFDLIENTLKTEFERPIIKSNNTKYFSKVIWTKKDSYD
                     VSIPISLFWKSEDTDELPRRKKCILSVYGAYGKNDNSDLDEIMLSIIDAGFIYAIVHV
                     RGGGYLGGEWYRSGKALNKWNSIRDFIEGVNYLRENDVIDSKRLGLITSSAGGIIAGA
                     VLNEEKNLLQSILLFSPFINPYDTLQNPNDPLSKTEIAEWGDIRDPEVKAYIKSYSPM
                     QNIEKARDSNTVIVNILGEKDPYINNNEVIEWSKKLNSIGVKSLLYLNKAAGHGGFTP
                     SDVLLMIDTLNYFFEEVGRN"
BASE COUNT         1633 a          581 c          934 g         1516 t
ORIGIN      
        1 gaatccaaag gattctccct ttttatttaa ataagtctgt ctagctatag atatattaag
       61 tttgataaaa tttcttggag attttgttat ggattataat ttcaatttgg agcatccttt
      121 ctttttcact aataatgatt atagtacaga tactagcata aagtaccaag taagtctgcc
      181 ttttaattgg cacgaagtaa tgaataatga tgagtgggtt tatcaatacc ctattggaaa
      241 atttgttgaa agacaagggt ggaaaataca cattagttca gagtataaca gttcacatga
      301 attgttacaa gatgtagcaa aaatttgcca tgaaatgcga attccattta aacatcttag
      361 tacagaagac aaatttatca tgcgaaacgg gaaattggtt agtcgaggat tttctggtaa
      421 atttattaca tgttatccaa atcaaaatga attagagtcg gttttgcaaa ggttagaaag
      481 tgccttaaag caatataatg ggccatatat tttaagcgat aaacgttggg atgaagcacc
      541 aatatatctg aggtatggtg tgtttaggcc gtcaagggat gatgaaaaga aggttgctat
      601 tgatgaatta attgttggcg atgaagttgt taaagatgaa cgtttaccag tatttaaaat
      661 tccaaaaggt atagtgcccc ctgacttttt gaataaatgg cttgacaaga aagataaaaa
      721 acagggagac tttcccttta ttatagataa tgcaattaga ttttcaaaca gtggtgggat
      781 atataatgct aggctaaaag aagatgggaa aaaaataatt ttaaaagagg ctagaccgta
      841 tacaggtcta ggatttgatg gtacttattc ttcagagaaa ttagcttcgg agtgcaaagc
      901 actaaaaatt ctaaatgaat ggagtgagac gcctaaaata tactggcacg gtaagatctg
      961 ggagcacacc ttcttaggaa ttgaacatat gaagggcgtt ccactaaata gatgggttac
     1021 caataatttt cctttatatg aagttgttga taagaccaaa gattatctat tgagagtttc
     1081 aaagatagta gaaaaactca tcgatttaac gaataagttt cattctgaaa atgtatatca
     1141 tcaagattta catttaggaa atatcttggt taaggatgaa gatgagatta gtataataga
     1201 ctgggaacag gctgtctttt caaatgatga aaaagttgta cataaagttg cagcccctgg
     1261 ttttagagca tggcgagaaa cacttcccag tgagatagat tggtatggta ttagacaaat
     1321 agctcattat ttatacatgc cactagtaac tacatcggat ttgacataca actatgtaag
     1381 tcaaaccagg atagaaggaa aaaaactatt tgagtctttg ggttatacaa gagaacatat
     1441 agattatgta gaaagtttat tgtcatatct agattctaaa tgtccacaga ttgagaatat
     1501 ttccaggaaa aaagttttaa aacccatgca tgagattaga actatcgaga gtgagcaaga
     1561 tatacaagat tttattataa aattattgag agggtttacg ctaacttatg gacagtggcg
     1621 taaagaattt caatcaagat tcttcccagt tcattattat ggtctgaatt ttaatcaagg
     1681 aattgcgttt tcagatttag ctatcctttg gagttatcaa caactagcta agaaagtaaa
     1741 aaatttcaag tttgatgact attatgagat tcgaacacag gttattaatg aggcagtaaa
     1801 taattttaag aaatcgtccc tttctggtct ttttgatggt aaaattggta caatctggct
     1861 catttatgaa tttggggaga tagatcgggc agttgaactt tttacaactc attttataga
     1921 gatatttgag aattcacaaa ataaaaattt atatagtggg caggctggaa ttttactggt
     1981 tggactgtat tttcttagta aagggggaat tgataataaa ctaggagaag aaatactaat
     2041 taggcttaga gaatatactt taaattatat agaaaatcca gaaacttttt gtaaagttgg
     2101 cgctagtgat gttcagagta acgatcctta tgagaatttt ggtggattac tatatggtca
     2161 cgcaggggta gcttggctct tcggtgaagc ttataaatta actggtgaaa gtatctataa
     2221 aaatggtcta gaattggctg tagataaaga attagttgct tataaagtag atagtaataa
     2281 ttcattgcag tacagccaag gtcatagatt actaccatat ctagcaactg gaagtgctgg
     2341 cctgctttta cttattaata gaaataaaga aatcttgtca agcaaatact taaaatattt
     2401 aacttcttta gagagggcaa ctgatgtagt tttttgtgta ttaccaggtt tatttaacgg
     2461 attttgtggt cttgaagttg ctaataatat atatagtgat atcgatgata acttttcagg
     2521 acaaaagaaa ttaattgaac aactttatag atatttgtgt gtaattgaag aaggatttgt
     2581 tattgcggga gataatggtt taaagataac aacagatatt gcttcgggat ttgcgggtgt
     2641 tgcaattgga ttggtatcaa taatggataa taaattgacg attcttcctc aaatataata
     2701 tttagatacc attattaagg ggtcatactt gtgaatcaga gtaatgaaat atttttaaat
     2761 acaatacagg aaaaaacaca ctataagagg tctatctctt catcatttca ggaattaaaa
     2821 aaatggaatt ctttgatatt taggagaggg cgatattggt accaatttga taattggagt
     2881 atacccaata ctggtgtaat aaaggaatgg tatgtcgaag ataatgactg gattcctaag
     2941 gttagttctt ccagtttaga caattcaaat tcacttaaaa ttgtctttga tgtggaaaaa
     3001 tatactttgc cagatgatag tatcttacag gatgttagga tttcatctga tgagagttat
     3061 atagttttgg ttgtatcctc actgcgtagc actaatttaa ttggaataaa aaaatatact
     3121 atggaagagt tatttatcat tgaagatatt tcagtagaaa gttcgttcta tttgggtaaa
     3181 ttcggtgtta tgtatactcg ctccaaagag tacgggaggc caagtaagtt attctataaa
     3241 tcttttgata gctttacaga agaggaactg tttgaagaaa acgaatgctc ttttcgatta
     3301 aaaatagtcc acattgatag caataattgt tttgtaaagt cagtagactt tcagaaaggc
     3361 aggatttttc tatacagttt cgaccggacg ggttttgtta gacactctta cacagaaacg
     3421 gtagccccaa cacctagaga tatcgctcta tttagtacag aaaaagcaca gtactttctt
     3481 gggctctctt caactgaaga aaagaaagat caactaattc taagggaaac ttcttcggga
     3541 gacagagttt ctattactat accatatcaa gatagggcta gaagagttca ctgtatagga
     3601 cgttatatcc ttttagactg ttcaaacgca gcgaattccg tcttttatct tgctacattt
     3661 aaaaataata gtttgaggga gttgagtgtt aataagataa catttgatga aaagacaaca
     3721 ctttatgaaa attcattttc tgatcgtgtt cttctgtttt tagagaggag aactttttac
     3781 gaaaaaatac ttagttttga tttaatagaa aatacattaa aaactgaatt tgaaagaccc
     3841 atcataaaat cgaataatac taaatatttt tctaaggtga tttggactaa aaaagacagt
     3901 tatgatgtaa gcataccgat ttctttattt tggaaaagtg aagatacgga tgagctccca
     3961 agaagaaaaa aatgtattct aagtgtctat ggggcttatg gtaagaatga taattctgat
     4021 ttagatgaaa ttatgttatc tataatagat gcaggtttta tttatgctat agttcatgta
     4081 agaggtggag gctatttggg tggtgaatgg tatcgctcag gaaaggcgtt aaacaaatgg
     4141 aattctatca gagattttat tgagggagta aattatttaa gggaaaatga tgtaattgac
     4201 agtaagcgat taggtttaat aacttctagt gcaggtggaa taattgctgg tgcggtgttg
     4261 aacgaggaaa aaaatttatt gcaaagcatt ctcttatttt cgccatttat aaatccttat
     4321 gatacacttc agaatccaaa tgatcctctt tctaagaccg aaatagcaga gtggggagat
     4381 attagagacc ctgaagtaaa ggcttatata aagtcatatt cgcctatgca aaatattgaa
     4441 aaggcacgag actcaaatac tgttatagtt aatattttgg gtgagaaaga cccatatatt
     4501 aataataacg aagtgataga gtggtcaaag aaattaaatt ctattggagt taaaagtttg
     4561 ttgtatttaa acaaagcagc tggtcatgga ggttttactc catcagatgt tctcttaatg
     4621 attgatactt taaattattt ctttgaagaa gtgggaagga ataa
//
LOCUS       DAVKNL010000099         4128 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVKNL010000099 DAVKNL010000000
VERSION     DAVKNL010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.099"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            720..1712
                     /locus_tag="VW411_002032"
     CDS             720..1712
                     /locus_tag="VW411_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9794678.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPDSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPRVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYDGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1714..2532
                     /locus_tag="VW411_002033"
     CDS             1714..2532
                     /locus_tag="VW411_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEU9794679.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVIYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2534..3319
                     /locus_tag="VW411_002034"
     CDS             2534..3319
                     /locus_tag="VW411_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9794680.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLTQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3358..3636
                     /locus_tag="VW411_002035"
     CDS             3358..3636
                     /locus_tag="VW411_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEU9794681.1"
                     /translation="MFVIEEVKDENQKKAVVAEVLKDLPEWFGIPESTQAYIEGTTTL
                     QVWTAYQESDLTRFVSLSYSSEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            3621..3818
                     /locus_tag="VW411_002036"
     CDS             3621..3818
                     /locus_tag="VW411_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9794682.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFPQLWNPQNPCQILIKKLE"
BASE COUNT         1153 a          702 c          893 g         1380 t
ORIGIN      
        1 gctatatatt atggtatcga atcttcatca gaatgataaa attaatcaat tgatatctga
       61 ttacaaacag aatatgaaag ctttttatat cactattgaa aaatttatac gagatgatga
      121 aagccttaag tgttatttta taaaggttat ttcaagtcgt tccaaggtaa caagtctaga
      181 tcagattgaa gctgataaaa cgatacaaag aaaatattca agtgagctaa aaaaatttat
      241 tggattttat aatgagatta tttgtgagga aaatagtttc ctacatgtac gaaagaggtg
      301 gtcgagttgg tttaggtagt cgatgcgtga gttgataatt ctcagggtat ggacttcttt
      361 ttcatgaatg aggtaaaaga gcaggtattg tttagagaca atcattctga gcatattttc
      421 tggatagagg gagtatccga ttttatgatc aaagttaata ccgccctctg gtgagaagat
      481 gagtaggttg gtaatttaaa ctattaaaca gaatttttga ttaaaagtat tatttcatga
      541 gagaaatcct aatttcacaa tccataggca aacgcttgca tttcgttttt tattggacta
      601 taataggttg gtataaagcc ttctgtagta ataaaatgta gaaggtgtag aaagtaagga
      661 tttagaatat ttgtagttaa aaacacaatg ttgctattcc ttacgatagg gagatagata
      721 tggcaatgat agaagtggaa catcttcaga aaaattttgt gaagactgtt aaggaaccgg
      781 gcttgaaggg ggctttgcgc tcctttattc atcctgaaaa gcagaccttt gaagcggtca
      841 aggatttgac ctttgaggtt ccaaaagggc agattttagg atttatcggg gcaaatggtg
      901 ctgggaagtc gacaaccatt aaaatgctga caggaatttt gaaaccaaca tctggttttt
      961 gtcggattaa cggcaagatt ccccaggaca atcggcaaga ttatgtcaaa gatattggcg
     1021 tagtctttgg acaacgcacc cagctatggt gggatttggc tctgcaagag acctacactg
     1081 tcttaaaaga gatttatgat gtgccagact cgctctttca taagcgtatg gactttttga
     1141 atgaagtctt ggatttgaag gactttatca aggatcccgt gcggactctt tcactgggac
     1201 aacggatgcg ggcggatatt gcggcctcct tgctccacaa tcccagggtt ctttttttag
     1261 atgagccgac cattggtttg gacgtttcgg ttaaggataa tattcgtcgg gcaattactc
     1321 agatcaatca agaggaagaa actaccattc ttttgaccac tcacgatttg agtgatattg
     1381 agcaactttg tgatcggatt ttcatgattg acaaggggca agagattttt gatggaacgg
     1441 tgagccaact caaggagacc tttggtaaga tgaagactct ctcttttgaa ctgctaccag
     1501 gtcaaagtca tctcgtctct cactatgacg gtctgtctga tatgaccatt gatagacaag
     1561 gaaacagcct caacattgaa tttgatagtt ctcgctacca gtcagctgac attatcaagc
     1621 aaaccctgtc tgattttgaa atccgcgatt tgaagatggt ggatacggat attgaggata
     1681 ttatccgtcg cttctaccga aaggagctct aggatgatca aattgtggag acgttataaa
     1741 ccctttatca atgcaggggt tcaggagttg attacttacc gagtcaactt tattctctat
     1801 cggattggcg atgtcatggg ggcttttgtg gccttttatc tctggaaggc tgtctttgat
     1861 tcttcgcaag agtctttgat tcagggcttc agtatggcgg atatcaccct ctacatcatc
     1921 atgagttttg tgaccaatct tctgactaga tccgattcgt cctttatgat tggggaggag
     1981 gtcaaggatg gctccattat catgcgtttg ttgcgaccag tgcattttgc ggcctcctat
     2041 cttttcaccg agcttggttc caagtggttg atttttatca gcgttggcct tccattttta
     2101 agtgtcattg tcttgatgaa aatcatatcg ggtcaaggta ttgtagaggt gctaggatta
     2161 actgtcattt atctttttag cttaacgctc gcctatctga ttaacttttt ctttaatatt
     2221 tgctttggat tttcagcctt tgtgtttaaa aatctttggg gttccaacct acttaagact
     2281 tccatagtgg cttttatgtc ggggagtttg attcccttgg cattttttcc aaaggttgtt
     2341 tcagatattc tctccttttt gcctttttca tccttgattt atactccagt tatgatcatt
     2401 gttggaaaat acgatgccag tcagattctt caggcactcc ttttgcagtt cttctggctc
     2461 ttagtgatgg tgggattgtc tcagttaatt tggaaacggg tccagtcctt tatcaccatt
     2521 caaggaggtt agtatgaaaa aatatcaacg aatgcatctg atttttatca gacaatacat
     2581 caaacaaatc atggaatata aggtagattt tgtggttggt gtcttgggag tctttctgac
     2641 tcaaggcttg aatctcttgt ttctcaatgt catctttcaa catattccat tcctagaagg
     2701 ctggaccttt caagagatag ctttcattta tggattttcc ttgattccca agggaatgga
     2761 ccatctcttt tttgacaatc tctgggcact agggcaacgc ctagtccgaa aaggggagtt
     2821 tgacaagtat ctgactcgtc ccatcaatcc tctctttcac atcctagttg aaacctttca
     2881 gattgatgcc ttgggtgaac tcttagtcgg tggtatttta ttgggaacaa cagtgaccag
     2941 cattgtttgg actcttccaa aattcctgct tttcctagtt tgtattcctt ttgcgacctt
     3001 gatttatact tctcttaaaa tcgcaacagc cagtatcgcc ttttggacta agcagtcagg
     3061 cgccatgatt tacatcttct atatgttcaa tgactttgct aagtatccga tttctattta
     3121 caattctctt cttcgttggt tgattagctt tatcgtgcct ttcgccttta cagcctacta
     3181 tccagctagc tatttcttac aggaaaagga tgtgttcttt aacgtaggag gtttgatgtt
     3241 gatttctctg gttttctttg ttatttccct taaactttgg gataagggct tagattccta
     3301 cgaaagtgcg ggttcgtaaa agctaaagta agactaaaat caagaaagaa acttatgatg
     3361 tttgtaattg aagaagtcaa ggatgaaaat caaaaaaagg cagttgtcgc tgaggttttg
     3421 aaggatttgc cagaatggtt tggaatccca gaaagcacac aagcctatat agaaggaacc
     3481 acgacactgc aagtttggac cgcctatcag gagagtgatt tgactagatt tgtaagctta
     3541 tcctattcga gtgaagattg tgcagagatt gattgtctcg gcgtaaaaaa gcttatcaag
     3601 gtagaaaaat tgggagccaa ttgcttgcta ctttagagag tgaagctcgt aaaaaagttg
     3661 gttatctgca ggtcaaaaca gtggcagaag gttctaataa agattatgat cgaacaaatg
     3721 acttttatcg aggtcttggc tttaaaaagt tagagatttt tcctcaacta tggaatccgc
     3781 aaaatccttg tcagattttg attaaaaagc ttgaataata ttacttgaca tctattctca
     3841 gagtgctata ctgtaagtgt aatcgccgat ttagcttagt tggtagagca aggcactcgt
     3901 aaagcctagg ttataggtag ataaacgact gaggatttga aaaaatagat aggtagaaga
     3961 taaccgttaa gccttactct tagcggttat ttatattgtt taatagcgct aatattttat
     4021 caattatgcc tgttttcgtg tttctggtag ttgttcaagt ttattgctac tatttttgat
     4081 ggtatgaatg tgcttataat gtatcccggt taacgaaagt tttggact
//
LOCUS       DAVKNL010000100         4077 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVKNL010000100 DAVKNL010000000
VERSION     DAVKNL010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.100"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            <1..1774
                     /locus_tag="VW411_002037"
     CDS             <1..1774
                     /locus_tag="VW411_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9794683.1"
                     /translation="TSASESASTSASASASTSASESASTSASASASTSASASASISAS
                     ESASTSASASASTSASASASTSASASASTSASESASTSTSASASTSASESASTSASAS
                     ASTSASASASTSASASASTSASASASTSASASTSASESASTSASASASTSASASASTS
                     ASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASESASTSAS
                     ASASTSASASASTSASASASTSASASASTSASVSASTSASESASTSASASASTSASAS
                     ASTSASESASTSASASASTSASESASTSASASASTSASASASTSASASASTSASASAS
                     TSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTS
                     ASASASTSASASASTSASVSASTSASASASTSASASASISASESASTSASASASTSAS
                     ASASTSASASASTSASASASISASESASTSASASASTSASASASTSASASASTSASAS
                     ASTSASASASTSASASASTSVSNSANHSNSQVGNTSGSTGKSQKELPNTGTESSIGSV
                     LLGVLAAVTGIGLVAKRRKRDEEE"
     gene            1835..3919
                     /locus_tag="VW411_002038"
     CDS             1835..3919
                     /locus_tag="VW411_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000341103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9794684.1"
                     /translation="MDDKITVIVPVYNVENYLRKCLDSIITQTYKNIEIVVVNDGSTD
                     ASGEICKEFSEMDHRILYIEQENAGLSAARNTGLNNMSGNYVTFVDSDDWIEQDYVET
                     LYKKIVEYQADIAVGNYYSFNESEGMFYFHILGDSYYEKVYDNVSIFENLYETQEMKS
                     FALISAWGKLYKARLFEQLRFDIGKLGEDGYLNQKVYLLSEKVIYLNKSLYAYRIRKG
                     SLSRVWTEKWMHALVDAMSERITLLANMGYPLEKHLAVYRQMLEVSLANGQASGLSDT
                     ATYKEFEMKQRLLNQLSRQEESEKKAIVLAANYGYVDQVLTTIKSICYHNRSIRFYLI
                     HSDFPNEWIKQLNKRLEKFDSEIINCRVTSEQISCYKSDISYTVFLRYFIADFVQEDK
                     ALYLDCDLVVTKNLDDLFATDLQDYPLAAVRDFGGRAYFGQEIFNAGVLLVNNAFWKK
                     ENMTQKLIDVTNEWHDKVDQADQSILNMLFEHKWLELDFDYNHIVIHKQFADYQLPEG
                     QDYPAIIHYLSHRKPWKDLAAQTYREVWWYYHGLEWTELGQNHHLHPLQRSHIYPIKE
                     PFTCLIYTASDHIEQIETLVQSLPDIQFKIAARVIVSDRLAQMTIYPNVTIFNGIHYL
                     VDVDNELVETSQVLLDINHGEKTEEILDQFANLGKPILSFENTKTYEVGQEAYAVDQV
                     QAMIEKLREISK"
BASE COUNT         1232 a          882 c          869 g         1094 t
ORIGIN      
        1 aaccagtgca tctgaatcgg catcaaccag tgcctcagct tcagcaagta cctcagcatc
       61 tgaatcagca tcaacaagtg catcggcttc agcaagcaca agtgcttcag cctcagcaag
      121 tatctcagcg tctgaatcgg catcaacgag tgcgtccgct tcagcaagta ctagcgcctc
      181 agcatcagcg tcaacaagtg cttcggcttc agcgtcaacg agtgcgtctg agtcagcatc
      241 aacgagtacg tcagcctcag caagcacatc agcttctgaa tctgcatcaa ccagtgcgtc
      301 agcctcagca tcgacaagcg cctcagcttc agcaagtacc agtgcgtcag cctcagcaag
      361 taccagtgct tcagcctcag cgtcgacaag tgcgtcggcc tcaaccagtg catctgaatc
      421 ggcatcaacc agtgcgtcag cctcagcaag tactagcgcc tcagcctcag catcaacgag
      481 tgcgtccgct tcagcaagta ctagtgcatc agcttcagca agtactagcg cctcagcctc
      541 agcgtcgaca agcgcctcag cttcagcaag taccagtgcg tcagcctcag cgtcgacaag
      601 tgcgtcggct tcagcaagta cctcagcgtc tgaatcagca tcaacaagtg cgtcggcttc
      661 agcatcaacg agtgcatcag cttcagcatc aacaagtgct tcagcttcag caagtaccag
      721 tgcgtcggct tcagcatcaa cgagtgcttc agtctcagcg tcaaccagtg cctctgaatc
      781 cgcatcaaca agtgcctcgg cttcagcaag caccagtgct tcggcttcag cgtcaacgag
      841 tgcgtctgag tcagcatcaa cgagtgcgtc agcctcagca agcacatcag cttctgaatc
      901 tgcatcaacc agtgcgtcag cttccgcatc aacaagcgcc tcggcctcag caagtacaag
      961 tgcttcagcc tcagcatcaa ccagtgcatc agcttcagcc tcaacaagtg cttcagcctc
     1021 agcgtcaacc agtgcctcgg cttcagcaag taccagtgcg tcagcttcag caagcacaag
     1081 tgcgtcagct tccgcatcaa ccagtgcttc ggcttcggca tcaacaagtg cctcagcatc
     1141 agcatcaacg agtgcgtcag cctcagcaag tactagtgca tcagcatcag catcaacgag
     1201 tgcatcggct tcagcgtcaa ccagtgcatc agtctcagca agcaccagtg cgtcggcttc
     1261 agcatcaacg agtgcctcag cctcagcaag tatctcagcg tctgaatcgg catcaacgag
     1321 tgcgtcagcc tcagcaagta ctagtgcatc ggcttcagca agcaccagtg cgtcggcttc
     1381 agcatcaacc agtgcctcag cctcagcaag tatctcagcg tctgaatcgg catcaacgag
     1441 tgcgtcagcc tcagcaagta ctagtgcatc agcatcagca tcaacgagtg catcggcttc
     1501 agcaagcacc agtgcctcgg cctcagcaag caccagtgct tcagcttcag catcaacaag
     1561 tgcgtcagct tcagcaagta catcagtttc aaattcagca aaccattcga actcacaagt
     1621 tggaaatact tctggatcga caggtaaatc ccaaaaagaa ttgcctaata caggtactga
     1681 gtcgtcaatt ggatctgtgt tacttggagt tctagcagct gttacaggta ttggattggt
     1741 tgcgaaacgc cgtaaacgtg atgaagaaga gtaagacaac ctgtaaagtt aggctaaact
     1801 aactcgcgca cataaatcaa ggagaaaatt gctagtggat gataaaataa cagtcattgt
     1861 accagtatac aatgtggaaa actatctgag gaagtgccta gatagtatta ttactcaaac
     1921 atataaaaat attgagattg ttgtcgttaa tgatggttct acggatgctt caggtgaaat
     1981 ttgtaaagaa ttttcagaaa tggatcaccg aattctctat atagaacaag aaaatgctgg
     2041 tctttctgcc gcacgaaaca ccggtctgaa taatatgtcc ggaaattatg tgacctttgt
     2101 ggactcggat gattggattg agcaagatta tgtagaaact ctatataaaa aaatagtaga
     2161 gtatcaggct gatattgcag ttggtaatta ttattctttc aacgaaagtg aaggaatgtt
     2221 ctactttcat atattgggag actcctatta tgagaaagta tatgataatg tttctatctt
     2281 tgagaacttg tatgaaactc aagaaatgaa gagttttgct ttgatatctg cttggggtaa
     2341 actctataag gcaagattgt ttgagcagtt gcgctttgac ataggtaaat taggagaaga
     2401 tggttacctc aatcaaaagg tatatttatt atcagaaaag gtaatttatt taaataaaag
     2461 tctttatgct tatcggatta gaaaaggtag tttatcaaga gtttggacag aaaagtggat
     2521 gcacgcttta gttgatgcta tgtctgaacg tattacgcta ctagctaata tgggttatcc
     2581 tctagagaaa cacttggcag tttatcgtca gatgttggaa gtcagtctcg ccaacggtca
     2641 agctagtggt ttatctgaca cagcaacgta taaagagttt gaaatgaaac aaaggctttt
     2701 aaatcagcta tcgagacaag aggaaagtga aaagaaagcc attgtcctcg cagcaaacta
     2761 tggctatgta gaccaagttt taacgacaat caagtctatt tgttatcata atcgttcgat
     2821 tcgtttttat ctgattcata gcgattttcc aaatgaatgg attaagcaat taaataagcg
     2881 cttagagaag tttgactcag aaattattaa ttgtcgggta acttctgagc aaatttcatg
     2941 ttataaatcg gatattagtt acacagtctt tttacgctat ttcatagctg atttcgtgca
     3001 agaagacaag gccctctact tggactgtga tctagttgta acgaaaaatc tggatgactt
     3061 gtttgctaca gacttacaag attatccttt ggctgctgtt agagattttg ggggcagagc
     3121 ttattttggt caagaaatct ttaatgccgg tgttctcttg gtaaacaatg ctttttggaa
     3181 aaaagagaat atgacccaaa aattaattga tgtaaccaat gaatggcatg ataaggtgga
     3241 tcaggcagat cagagcatct tgaatatgct ttttgaacat aaatggttgg aattggactt
     3301 tgattataat catattgtca ttcataaaca gtttgctgat tatcaattgc ctgagggtca
     3361 ggattatcct gctattattc actatctttc tcatcggaaa ccgtggaaag atttggcggc
     3421 ccaaacctat cgtgaagttt ggtggtacta tcatgggctt gaatggacag aattgggaca
     3481 aaaccatcat ttacatccat tacaaagatc tcacatctat ccaataaagg aacctttcac
     3541 ttgtctaatc tatactgcct cagaccatat tgaacaaatt gagacattgg ttcaatcctt
     3601 gcctgatatt cagtttaaga tagcagctag agtaatagtt agtgatcgat tggctcagat
     3661 gacaatttat ccaaacgtga ctatatttaa cggaattcac tatttggtag atgtcgataa
     3721 tgaattggta gaaaccagtc aagtactttt agatattaat catggcgaaa agacagaaga
     3781 aattctcgat caatttgcta atcttggcaa gcctatctta tcctttgaaa atactaaaac
     3841 ctatgaagta ggtcaggagg catatgctgt tgaccaagtt caagcaatga ttgaaaaatt
     3901 gagagaaata agcaaatgaa gaaaaatcat ttagtaggag atgctctgat tttgacggtt
     3961 agtgatcaga ttgaagagtt ggattatttt ttataaaatt tctccgttca tcatatatga
     4021 aagttgttca aacatcagag tgctttataa aatataaata gacctaaaga tatttaa
//
LOCUS       DAVKNL010000101         4033 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVKNL010000101 DAVKNL010000000
VERSION     DAVKNL010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.101"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            591..818
                     /locus_tag="VW411_002039"
     CDS             591..818
                     /locus_tag="VW411_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794685.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            1106..4003
                     /gene="nanA"
                     /locus_tag="VW411_002040"
     CDS             1106..4003
                     /gene="nanA"
                     /locus_tag="VW411_002040"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HEU9794686.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPKGRVRLYVNGVLSRTSLRSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGNPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGDQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLDGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NRRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHEGKEYIILSNAGGPKRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKNLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKKDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
BASE COUNT         1356 a          680 c          889 g         1108 t
ORIGIN      
        1 atgtgtacta ttctagtgtc aatctattat atttatagaa ttttttgttg ctagatttgt
       61 caaattgctt aaaataattt ttttcagaaa gcaaaagccg atacctatcg agtagggtag
      121 ttcttgctat cgtcaggctt gtctgtaggt gttaatactt ttcaaaaatc tcttcaaacc
      181 acgtcagctt cgccttgccg taggtatggt tactgacttc gtcagttcta tccacaacct
      241 caaaacagtg ttttgagcat catgcggcta gcttcttagt ttgctctttg attttcattg
      301 agtataaaaa cagatgagtt tctgttttct ttttatggac tataaatgtt cagctgaaac
      361 tactttcaag gacattatta tataaaagaa ttttttgaaa ctaaaatcta ctatattaca
      421 ctatattgaa agcgttttaa aaatgaggta taataaattt actaacgctt ataaaaagtg
      481 atagaatcta tttttatgta tatttaaaga tagattgctg taaaaatagt agtagctatg
      541 cgaaataaca gatagagaga agggattgaa gcttagaaaa ggggaataat atgatattta
      601 aggcattcaa gacaaaaaag cagagaaaaa gacaagttga actacttttg acagtttttt
      661 tcgacagttt tctgattgat ttatttcttc acttatttgg gattgtcccc tttaagctgg
      721 ataagattct gattgtgagc ttgattatat ttcccattat ttctacaagt atttatgctt
      781 atgaaaagct atttgaaaaa gtgttcgata aggattgagc aggaagtatg gtgtaaatag
      841 cataggctga tgtccatcat ttgcttataa agagatattt tagtttaatt gcagcggtgt
      901 cctggtagat aaactagatt ggcaggagtc tgattggaga aaggagaggg gaaaattggc
      961 accaatttga gatagtttgt ttagttcatt tttgtcattt aaatgaactg tagtaaaaga
     1021 aagttaataa aagacaaact aagtgcattt tctggagtaa atgtcttatt tcagaaatcg
     1081 ggatatagat atagagagga tcagtatgaa tcggagtgtt caagaacgta agtgtcgtta
     1141 tagcattagg aaactatcgg taggagcggt ttctatgatt gtaggagcag tggtatttgg
     1201 aacgtctcct gttttagctc aagaaggggc aagtgagcaa cctctggcaa atgaaactca
     1261 actttcgggg gagagctcaa ccctaactga tacagaaaag agccagcctt cttcagagac
     1321 tgaactttct ggcaataagc aagaacaaga aaggaaagat aagcaagaag aaaaaattcc
     1381 aagagattac tatgcacgag atttggaaaa tgtcgaaaca gtgatagaaa aagaagatgt
     1441 tgaaaccaat gcttcaaatg gtcagagagt tgatttatca agtgaactag ataaactaaa
     1501 gaaacttgaa aacgcaacag ttcacatgga gtttaagcca gatgccaagg ccccagcatt
     1561 ctataatctc ttttctgtgt caagtgctac taaaaaagat gagtacttca ctatggcagt
     1621 ttacaataat actgctactc tagaggggcg tggttcggat gggaaacagt tttacaataa
     1681 ttacaacgat gcacccttaa aagttaaacc aggtcagtgg aattctgtga ctttcacagt
     1741 tgaaaaaccg acagcagaac tacctaaagg ccgagtgcgc ctctacgtaa acggggtatt
     1801 atctcgaaca agtctgagat ctggcaattt cattaaagat atgccagatg taacgcatgt
     1861 gcaaatcgga gcaaccaagc gtgccaacaa tacggtttgg gggtcaaatc tacagattcg
     1921 gaatctcact gtgtataatc gtgctttaac accagaagag gtacaaaaac gtagtcaact
     1981 ttttaaacgc tcagatttag aaaaaaaact acctgaagga gcggctttaa cagagaaaac
     2041 ggacatattc gaaagcgggc gtaacggtaa cccaaataaa gatggaatca agagttatcg
     2101 tattccagca cttctcaaga cagataaagg aactttgatc gcaggtgcag atgaacgccg
     2161 tctccattcg agtgactggg gtgatatcgg tatggtcatc agacgtagtg aagataatgg
     2221 taaaacttgg ggtgaccgag taaccattac caacttacgt gacaatccaa aagcttctga
     2281 cccatcgatc ggttcaccag tgaatatcga tatggtgttg gttcaagatc ctgaaaccaa
     2341 acgaatcttt tctatctatg acatgttccc agaagggaag ggaatctttg gaatgtcttc
     2401 acaaaaagaa gaagcctaca aaaaaatcga tggaaaaacc tatcaaatcc tctaccgtga
     2461 aggagaaaag ggagcttata ccattcgaga aaatggtact gtctatacac cagatggtaa
     2521 ggcgacagac tatcgcgttg ttgtagatcc tgttaaacca gcctatagcg acaagggtga
     2581 tctatacaag ggtgaccaat tactaggaaa tatctacttc acaacaaaca aaacttctcc
     2641 atttagaatt gccaaggata gctatctatg gatgtcctac agtgatgacg acgggaagac
     2701 atggtcagct cctcaagata ttactccgat ggtcaaagcc gattggatga aattcttggg
     2761 tgtaggtcct ggaacaggaa ttgtacttcg gaatgggcct cacaagggac ggattttgat
     2821 accggtttat acgactaata atgtatctca cttagatggc tcgcaatctt ctcgtgtcat
     2881 ctattcagat gatcatggaa aaacttggca tgctggagaa gcggtcaacg ataaccgtca
     2941 ggtagacggt caaaagatcc actcttctac gatgaacaat agacgtgcgc aaaatacaga
     3001 atcaacggtg gtacaactaa acaatggaga tgttaaactc tttatgcgtg gtttgactgg
     3061 agatcttcag gttgctacaa gtaaagacgg aggagtgact tgggagaagg atatcaaacg
     3121 ttatccacag gttaaagatg tctatgttca aatgtctgct atccatacga tgcacgaagg
     3181 aaaagaatac atcatcctca gtaatgcagg tggaccgaaa cgtgaaaatg ggatggtcca
     3241 cttggcacgt gtcgaagaaa atggtgagtt gacttggctc aaacacaatc caattcaaaa
     3301 aggagagttt gcctataatt cgctccaaga attaggaaat ggggagtatg gcatcttgta
     3361 tgaacatact gaaaaaggac aaaatgccta taccctatca tttagaaaat ttaattggga
     3421 atttttgagc aaaaatctga tttctcctac cgaagcgaaa gtgaagcgaa ctagagagat
     3481 gggcaaagga gagatgggca aaggagttat tggcttggag ttcgactcag aagtattggt
     3541 caacaaggct ccaacccttc aattggcaaa tggtaaaaca gcgactttcc taacccagta
     3601 tgatagcaag accttgttgt ttgcagtaga taaggaagat atcggacagg aaattattgg
     3661 tatagctaaa ggaagcatcg aaagtatgca taatcttcct gtaaatctag caggtgccag
     3721 agttcctggc ggagtaaatg gtagcaaagc agcggtgcat gaagttccag aatttacagg
     3781 gggagttaat ggtacagagc cagctgttca tgaaatcgca gagtataagg gatctgattc
     3841 gcttgtaact cttactacaa aaaaagatta tacttacaaa gctcctcttg ctcagcaggc
     3901 acttcctgaa acaggaaaca aggagagtga cctcctagct tcactaggac taacagcttt
     3961 cttccttggt ctgtttacgc tagggaaaaa gagagaacaa taagagaaga attctaaaca
     4021 tttgattttg taa
//
LOCUS       DAVKNL010000102         4032 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVKNL010000102 DAVKNL010000000
VERSION     DAVKNL010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.102"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            1..874
                     /locus_tag="VW411_002041"
                     /pseudo
     CDS             1..874
                     /locus_tag="VW411_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(1065..1352)
                     /gene="tnpB"
                     /locus_tag="VW411_002042"
     CDS             complement(1065..1352)
                     /gene="tnpB"
                     /locus_tag="VW411_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU9794687.1"
                     /translation="MIIQLSDLGQVHLVCGKTDMRQGIDSLAYVVKTHFELDSFSGQV
                     FLFCGGRKDRFKALYWDGQGFWLLYKRFENGKLTWLSTEKDVKALAPEQVD"
     gene            1593..2930
                     /locus_tag="VW411_002043"
                     /pseudo
     CDS             1593..2930
                     /locus_tag="VW411_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3039..3338)
                     /locus_tag="VW411_002044"
     CDS             complement(3039..3338)
                     /locus_tag="VW411_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEU9794688.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKASVDEANIKTLYSEKLIGISIPIK"
     gene            complement(3503..3954)
                     /locus_tag="VW411_002045"
                     /pseudo
     CDS             complement(3503..3954)
                     /locus_tag="VW411_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
BASE COUNT         1340 a          686 c          793 g         1213 t
ORIGIN      
        1 gaaatgtaaa aaaatatgag gagttcggac tcgactctct ccttcaagaa acacgtggtg
       61 gtcgtaacca tgcatatatg acagttgagg aaaagaaagt ctttcttgcc cgccatttga
      121 aggctgcaga ggcaggagaa tttgttacaa ttgatgcctt atttcaggct tataaaaagg
      181 agttaggtcg ttcctacaca cgtgatgcct tctatcaact gttgaagtgc catggttggc
      241 gaaatattat gccacgtcca gaacatccta agaaagcaga cgctcaaacc attgtcgcgt
      301 ctaaaaataa aatctcaatt caagaagaaa agaaagcgct ttaaaaccag tagacgtttt
      361 cgtaaggttc gcttgatgta ccaagatgag gctggtttcg gtagaatcag taaactggga
      421 tcttgttggg ctccaatagg agtaggtcca catatccata gtcactatat acgagaattt
      481 cgctattgtt atggagctgt tgatgcccat acaggcgaat catttttctt aatagctggt
      541 agatgtaata ctgagtggat gaacgccttt ttagaagagc tttcacaagc ttatcctttt
      601 actcgttatg gacaatgcta tatggcataa atcaagtacc ttaaagattc cgactaatat
      661 tggttttacc tttattcctc catacacacc agagatgaac cccattgaac aagtgtggaa
      721 agagattcgt aaacgtggat ttaagaataa agcctttcaa actttggaag atgtcatgaa
      781 tcaactccaa gatgttatac aaggattgga gaaggaggtg ataaagtcca tcgttaatcg
      841 gagatggact agaatgcttt ttgaaaacag atgagtataa aaagaaagtc ctcatttcaa
      901 tagaaatcac gactttctga tggatttata gtaaaatgaa ataagaacag gacaaatcga
      961 tcaggacagt caaatcgatt tctaacaatg ttttagaagc agaggtgtac tattctagtt
     1021 tcaatctact atatttttgg agtgatagaa aagcccttca taagctagtc tacttgttca
     1081 ggtgcgagag ctttgacatc tttttctgta cttagccaag tcagttttcc gttctcaaag
     1141 cgtttatata gtagccaaaa tccttgacca tcccagtaaa gggctttaaa gcggtcttta
     1201 cgtccaccac aaaagagaaa gacttgaccg gagaaagaat ccaattcaaa gtgggtttta
     1261 actacatagg ctaatgagtc tattccctgc ctcatatctg tcttgccaca aacaaggtga
     1321 acttgaccta aatcacttag ttgaattatc atagtacaat acctttcctc cgataattat
     1381 tttttatcta gtatactgga agttggggaa ttaggataga taccttgtta tgacgcgctt
     1441 acgtaacttg taactagctg cctagtttga tctttgcttc ttcattgatt agcagtagat
     1501 ttcaaaatga taaaaacgca tagtatcagg tattgaaatg tactgcccca aaagttagac
     1561 agaaaaaatc taacttttgg ggtgtttttg ttatgaaatt aagttatgat gataaagttc
     1621 agatctatga acttagaaaa caaggatata gcttagagaa gctttcaaat aaatttggga
     1681 taaataattc taatcttagg tatatgatta aattgattga tcgttacgga atagagttcg
     1741 tcaaaaaagg aaaaaatcgt tactattttc ctgatttaaa acaagaaatg attaataaag
     1801 tcttacatga aggctgaact aaagatagag tttctcttga atacggtctc ccaagtcgta
     1861 cgatacttct taactggcta gcacaataca gaaaaaacgg gtatactatt gttgagaaaa
     1921 caagagggag agtacctaaa atgggacgta agccaaaaaa gagacctgaa gagagaacag
     1981 aattagaacg tcttcaagca gaaaatgagt acctgagagc ggagaatgct atcctaaaaa
     2041 agttaagaga actccgatgg aaggaggaaa aagagaaaga agaaagacag aaattgttca
     2101 agaattaatg actgagtttt cgttagatct tcttctaaaa gccattaaac tagctcgttg
     2161 gacctactac tatcacttga aacagctaga caaaccagat aaggaccaag agcttaaagc
     2221 tgaaattcaa tccattttta tcgaacacaa gggaaattat ggttatcgtc ggatttattt
     2281 agaactaaga aatcgtggtt atctggtaaa tcataaaaga gttcaaggct tgatgaaagt
     2341 actcaattta caagctaaaa tgcgacagaa acgaaaataa aggagacgtt ggtaagaagg
     2401 cagagaatct cattcaaggc caatttgaag gctctaaaac aatggaaaag tgctacacag
     2461 atgtgacaga atttgccatt ccagcaagta ctcaaaagct ttacttatca ccagttttag
     2521 atggctttaa tagcgaaatt attgctttta atctttcgac ttcacccaac ttagaacaag
     2581 tacaaacaat gttagaacag gcattcaaag agaagcacta cgagaatacg attctccata
     2641 gtgaccaagg ctggcaatat caacacgatt cttatcatcg gttcctagag agtaagggaa
     2701 ttcaagcatc tatgtcacgc aagggcaaca gccaagacaa cggtaggatg gaatctttct
     2761 ttggcatttt aaaatccgaa atgttttatg gctatgagaa aacatttaaa tcacttaacc
     2821 aattggaaca agccattata gactatattg attactacaa caacaaacga attaaggtaa
     2881 aactaaaagg acttagtcct gtgcagtaca gaactaaatc ctttggataa attaattgtc
     2941 taactttttg gggtcagtac aaaactcttg ctactatgcg ttttattatt gaaagactta
     3001 ttggactttc tctcaaatcg agtttttact caattttctt acttgattgg gattgaaatt
     3061 ccaattaatt tctctgagta gagtgtcttg atattggctt catcaacaga ggccttatca
     3121 attttacgtt tcaagaaaaa ttcttgaatg gtttcgattt caggctcacg aatagcacgg
     3181 tgtttgtttg agatgaggat ttcatagtga agcggagctt gggtaaaaat aacatctgta
     3241 ttccctgcag aataaacctc aacaagggtt gcatcggtac tttctagctg actttttaca
     3301 agttgcgagt gtgagtttgt cgtattgata agcttcataa tatttcctcc gattttctaa
     3361 ttctattata gcactttttg aataaagtcg cttgatttat actcaatgaa aatcaaagag
     3421 caaactagga agctagccgc aggctatact tgagtacggt aaggcgacgc tgacgtggtt
     3481 tgaattttat tttcgaagag tattagccaa tcttatgctg ttttttccaa gattcaatgg
     3541 cccatttatg gctaccacgt ttaaggtttt tgatagcctc gtcaataggg aaccaggcaa
     3601 tatgattaaa gttttctagt ggcttttgta cttctttgaa aggagttgct tcatagaggt
     3661 aggcaggatt gtagtagtag gtatcacgat gacgagaata gaaatattcg tcagcttgtc
     3721 cgtaataggt accaatttct gctgtgaaac caagctcttc aatcaactca tgctttaggg
     3781 cttcctgatg attttcacct gcttcaattt ctccacatgg taggaaccaa gcaccatttg
     3841 gttcttgaac aagaacaatt tgtttttgtt caggattagg gataactgca tatacgccat
     3901 agcgagcaat atagtctgta ttcacttttt ttctccgaaa gttgggtttg ccattgcatt
     3961 ttcctcatta tctagtatcg ttattattat agtgaaatga accaaaaata gtacacaatg
     4021 tggtataatc tt
//
LOCUS       DAVKNL010000103         3956 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVKNL010000103 DAVKNL010000000
VERSION     DAVKNL010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.103"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            187..498
                     /locus_tag="VW411_002046"
     CDS             187..498
                     /locus_tag="VW411_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794689.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEYPELYAVAQTSGEIPEKKREELRQIITTIFEQKMNKHSI
                     K"
     gene            502..1218
                     /locus_tag="VW411_002047"
     CDS             502..1218
                     /locus_tag="VW411_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9794690.1"
                     /translation="MKKYFIGGLGSNAYHSKDFLQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSLGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIVSDLDVLTSKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNEATWREEALKELPDYFDTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(1347..2162)
                     /locus_tag="VW411_002048"
     CDS             complement(1347..2162)
                     /locus_tag="VW411_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEU9794691.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKITSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWITAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(2159..3064)
                     /locus_tag="VW411_002049"
     CDS             complement(2159..3064)
                     /locus_tag="VW411_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEU9794692.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLVYSLLIGVIL"
     gene            3406..>3956
                     /locus_tag="VW411_002050"
     CDS             3406..>3956
                     /locus_tag="VW411_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9794693.1"
                     /translation="MLHQGDKNSLLESLSIVERQTLKDIEVIIVNQSTEEIASISQQM
                     NLSFNIKIIDENSFAELSDMITGDYITFLSSNDSLFDWTFEKMYHAIKLSDSDVVLGH
                     FADLVDGVFYFYPFWGDNWLTENLTNVQVLQKMDDTDHRIRKQYRSLFGKLFNSKLLR
                     KINQFDINNLIWRLYIHSKTATY"
BASE COUNT         1349 a          627 c          792 g         1188 t
ORIGIN      
        1 ttaaatcgtg catcagttag ttgtttactt gcttcataat tcatagaact actataccat
       61 attttgtttc gcaggaagtc taatgctaaa atcttatcag tgagaagatt tcttaaagtt
      121 tattctttgt agtttcatct gtattagtac ataatttaac ttagagaaaa taggaggact
      181 aatcgaatgg aattaaaaga ttttacagaa aaagaacagg aaatgattaa gaaaaggctt
      241 acaatgtcta atattagtga caaagaaact actgagaaga ttctggcgct agtcccacaa
      301 gacttgatta agcgaatccc gttttttgtc agaaaacatg ctacaacacg tacgattaaa
      361 cgcatttcaa ttgaataccc tgaactctac gctgtagctc aaacaagtgg tgagattcca
      421 gaaaaaaaac gcgaagaatt gcgtcagatt atcacaacta tctttgaaca aaagatgaat
      481 aagcatagta ttaagtagag aatgaaaaaa tattttattg gcggtttggg aagcaatgcc
      541 tatcatagca aggattttct tcaagaacta gattcgcagg tctattttct aaatccatat
      601 gaaaagcatc ttcgagatga aacagaattg aaatcatggt ttaaaaatga aattgtagag
      661 gaagaatcta tctgtctgat aggccattct cttggaggag atttagctcg ttatttcgca
      721 tcggaatttg aagaagtaaa gaaactgatt cttttggatg gtggctatct agatttagat
      781 aagattttac ctttggatac agagttagag gaaactaaaa attatatcaa atctcaaatt
      841 gtttcggact tagatgttct tacttctaaa gaaaaatctg aagcaaagca ttggtcagaa
      901 aatatggaga aagctgtaag acagtcttat cactggaatg ttgagtataa tagatatgag
      961 ttggctataa attatgaaaa tatagaagcg atactccgcc tacggagaaa aatacaagct
     1021 tttaagagag aagtgggaga taccttgttt attagtcctc gctatcctaa tgaagctaca
     1081 tggagagagg aagccctaaa agaattgcca gactattttg atactatttt tttagaaaac
     1141 tttggccatg agctttatac tcaagcacct aaagaaatcg ctagtctaat gaatgagtgg
     1201 ctcgcttatt ttctatgagc tacttgtatt gctgggaatg gtataagatg aaaatctaca
     1261 agtcactaat tttcatataa caaaaaacga acaggctctt tttctgttat tcagaagaga
     1321 acttgttcgt ttttatcttg attttcttaa aaagctctat atacataggg attttctggt
     1381 ttatcgactt tggtaaagct gtcgatttcc aaggttggaa ggttttgttt gagttcttta
     1441 tggtagtgat tgaccagttt tcctttggct gttatccaag tgttgttttc atactgccgg
     1501 gtattgccct tggtcagcaa tccatagaca ccagaatctg cgatacagtg gataatgccg
     1561 aatcggaaca gaaattgact attggcatga ctggggtcgt tatagacaaa gcctgtaaac
     1621 tggattgtct tgccctcaaa ctcatctgga tagtcgtaga tagcctccat gacttccata
     1681 tagttttcat tagtgatctg aatactatct tgggataagt atttatccgc cgccgttcgc
     1741 atttcctttt cataggctga ttttgaaaaa taagaactgg tatctggttt caaatattgg
     1801 cttgtcgtcc cttcgcttgt ctgaatggct agatccgttc cttccgatag ggggaaatga
     1861 taacctttag cagaaacagt ctgagaatcc aagctaacag ttgggaaagt taagccgatg
     1921 acaagtggaa tagccagaag agaaatactc gttatcttgg ctaatcggct gttcagatga
     1981 ctgtgggttt tgacttgctt catccagata tacaattgaa cgatagccaa gataaaagaa
     2041 agcaccatgg agatataggc cagataggaa tagtgcatgt tgatgtactg gtttagtttg
     2101 cccgacagat ggaggtaaat agtcagttca aaatagccag ctaaaactaa aaatcgaatc
     2161 ataggataac tccaatcaag agagaataga ctaagacgac aagagttaca attgttatga
     2221 agtgactgat aaatcgtgct ttcaagtaat ttttcatcat gagaatattt ttgatatcca
     2281 gcattggacc aatgacgaga aaggccagaa ctggtgccaa accgaaactc gagagaagag
     2341 aagcacctat aaaggcatcc gcctcactac agagcgaaag aagaaaggct aaaatcatca
     2401 agagcaggat ggcaaaaaga ggggtcgcac tgatagaggt cagaatccga gtcggaacgt
     2461 agacctgtat tatagaagca aagaggcagc caaataccaa ataacgcccc gtatcaaaaa
     2521 attcatcaat ggcctgcaca aagacttgaa aaactttttt tgcagaactc aagtaagaaa
     2581 aatcatgctc atgacaagcc agacgatttt ctttctgaat cggttcttgc cagaaaaatc
     2641 ctagaaaaat tcctagtatt acagccacaa gaatggaacc cagagctcgt aatagggcga
     2701 catggaagga gttgccaaag gcagaatagg tcgcaaaaag aacaatggga ttgataacag
     2761 gtgctgtcac aagaaaagga acggccgtgt aacttggaac ctttttttcc agaaaacgat
     2821 tgatgatggg gacgattcca cattcacaag aagggaaaag tataccgaca aaggtcccaa
     2881 aaaagattct cccccaacga tttcgaggga gaaaatgata aaccttgtca ggtgtgatat
     2941 aaacttcaat cagccctgag acaatgcttc ctatcagaac gaagggaagg gcttcaatga
     3001 taatggagag aaaaatggct ccagtttgaa gtacactaga tgggagttgt tgaaaagcag
     3061 tcatctagtt tttcttttct gctttgactt ttttctcttt gtcatcaggg aaagtaactt
     3121 gtttcaaatc agggagttta gcaaactctt caaacatctt gtccaagtcg tctcgttttg
     3181 taaatttgac agccataggt gtacctcgat tctagattct acctctatta tactattatt
     3241 ttgcggaaaa gggaaaagtt aaaatttcaa attgagtagc taagagataa tatagcttga
     3301 tattacctga tatatatcat aattgtgcta taattaaata aaaagaaaat cattatcaga
     3361 aaattatggg aggtagtcaa actgacttac tcaattagtg ttgttttgtt acatcaagga
     3421 gataaaaata gtttattaga gtctttgtca atagtggaga gacaaacatt aaaagatata
     3481 gaagttatta ttgtaaatca atctacagag gagatagctt ccattagcca acagatgaat
     3541 ctatcattta atattaagat tatagatgag aattcttttg ctgagttgtc tgatatgatt
     3601 acaggagatt atattacgtt tctatcttca aatgattcat tatttgattg gacatttgaa
     3661 aagatgtatc atgcgataaa attatccgat tctgatgttg ttcttggtca ttttgctgat
     3721 ttggttgatg gagtatttta cttttatcct ttttgggggg ataattggtt gacagaaaat
     3781 ttaacgaatg ttcaagtttt acaaaagatg gatgatacag atcatcgaat tagaaaacaa
     3841 tatcgctcat tatttggaaa attattcaat agtaagcttt tacgaaaaat aaatcaattt
     3901 gatataaaca accttatttg gagactttat attcactcta aaactgcaac ctatat
//
LOCUS       DAVKNL010000104         3868 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVKNL010000104 DAVKNL010000000
VERSION     DAVKNL010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.104"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            <1..418
                     /locus_tag="VW411_002051"
     CDS             <1..418
                     /locus_tag="VW411_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEU9794694.1"
                     /translation="KNKVDQDSKPDEDKEHDEVSEPTHPESDEKENHAGLNPSADNLY
                     KPSTDTEETEEEAEDTTDEAEIPQVENSVINAKIADAEALLEKVTDPSIRQNAMETLT
                     GLKSSLLLGTKDNNTISAEVDSLLALLKESQPAPIQ"
     gene            628..3747
                     /gene="phtE"
                     /locus_tag="VW411_002052"
     CDS             628..3747
                     /gene="phtE"
                     /locus_tag="VW411_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEU9794695.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENLKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         1450 a          630 c          752 g         1036 t
ORIGIN      
        1 taaaaataag gtagaccaag acagtaaacc tgatgaagat aaggaacatg atgaagtaag
       61 tgagccaact caccctgaat ctgatgaaaa agagaatcac gctggtttaa atccttcagc
      121 agataatctt tataaaccaa gcactgatac ggaagagaca gaggaagaag ctgaagatac
      181 cacagatgag gctgaaattc ctcaagtaga gaattctgtt attaacgcta agatagcaga
      241 tgcggaggcc ttgctagaaa aagtaacaga tcctagtatt agacaaaatg ctatggagac
      301 attgactggt ctaaaaagta gtcttcttct cggaacgaaa gataataaca ctatttcagc
      361 agaagtagat agtctcttgg ctttgttaaa agaaagtcaa ccggctccta tacagtagta
      421 aaatgaatgg agcatatttt atggagaagt aacctttcgt gttacttctc ttttttagaa
      481 aaacgtaaca gaaacttgac aaaagtaatt ttaaatagta aactatttac tggttaatta
      541 aatggttaaa taaccggttt agaaaactat ttaataaagt aaaagaagtt gagaaaaaac
      601 ttcatcattt attgaaatga gggatttatg aaatttagta aaaaatatat agcagctgga
      661 tcagctgtta tcgtatcctt gagtctatgt gcctatgcac taaaccagca tcgttcgcag
      721 gaaaataagg acaataatcg tgtctcttat gtggatggca gccagtcaag tcagaaaagt
      781 gaaaacttga caccagacca ggttagccag aaagaaggaa ttcaggctga gcaaattgta
      841 atcaaaatta cagatcaggg ctatgtaacg tcacacggtg accactatca ttactataat
      901 gggaaagttc cttatgatgc cctctttagt gaagaactct tgatgaagga tccaaactat
      961 caacttaaag acgctgatat tgtcaatgaa gtcaagggtg gttatatcat caaggtcgat
     1021 ggaaaatatt atgtctacct gaaagatgca gctcatgctg ataatgttcg aactaaagat
     1081 gaaatcaatc gtcaaaaaca agaacatgtc aaagataatg agaaggttaa ctctaatgtt
     1141 gctgtagcaa ggtctcaggg acgatatacg acaaatgatg gttatgtctt taatccagct
     1201 gatattatcg aagatacggg taatgcttat atcgttcctc atggaggtca ctatcactac
     1261 attcccaaaa gcgatttatc tgctagtgaa ttagcagcag ctaaagcaca tctggctgga
     1321 aaaaatatgc aaccgagtca gttaagctat tcttcaacag ctagtgacaa taacacgcaa
     1381 tctgtagcaa aaggatcaac tagcaagcca gcaaataaat ctgaaaatct ccagagtctt
     1441 ttgaaggaac tctatgattc acctagcgcc caacgttaca gtgaatcaga tggcctggtc
     1501 tttgaccctg ctaagattat cagtcgtaca ccaaatggag ttgcgattcc gcatggcgac
     1561 cattaccact ttattcctta cagcaagctt tctgccttag aagaaaagat tgccagaatg
     1621 gtgcctatca gtggaactgg ttctacagtt tctacaaatg caaaacctaa tgaagtagtg
     1681 tctagtctag gcagtctttc aagcaatcct tcttctttaa cgacaagtaa ggagctctct
     1741 tcagcatctg atggttatat ttttaatcca aaagatatcg ttgaagaaac ggctacagct
     1801 tatattgtaa gacatggtga tcatttccat tacattccaa aatcaaatca aattgggcaa
     1861 ccgactcttc caaacaatag tctagcaaca ccttctccat ctcttccaat caatccagga
     1921 acttcacatg agaaacatga agaagatgga tacggatttg atgctaatcg tattatcgct
     1981 gaagatgaat caggttttgt catgagtcac ggagaccaca atcattattt cttcaagaag
     2041 gacttgacag aagagcaaat taaggctgcg caaaaacatt tagaggaagt taaaactagt
     2101 cataatggat tagattcttt gtcatctcat gaacaggatt atccaagtaa tgccaaagaa
     2161 atgaaagatt tagataaaaa aatcgaagaa aaaattgctg gcattatgaa acaatatggt
     2221 gtcaaacgtg aaagtattgt cgtgaataaa gaaaaaaatg cgattattta tccgcatgga
     2281 gatcaccatc atgcagatcc gattgatgaa cataaaccgg ttggaattgg tcattctcac
     2341 agtaactatg aactgtttaa acccgaagaa ggagttgcta aaaaagaagg gaataaagtt
     2401 tatactggag aagaattaac gaatgttgtt aatttgttaa aaaatagtac gtttaataat
     2461 caaaacttta ctctagccaa tggtcaaaaa cgcgtttctt ttagttttcc gcctgaattg
     2521 gagaaaaaat taggtatcaa tatgctagta aaattaataa caccagatgg aaaagtattg
     2581 gagaaagtat ctggtaaagt atttggagaa ggagtaggga atattgcaaa ctttgaatta
     2641 gatcaacctt atttaccagg acaaacattt aagtatacta tcgcttcaaa agattatcca
     2701 gaagtaagtt atgatggtac atttacagtt ccaacctctt tagcttacaa aatggccagt
     2761 caaacgattt tctatccttt ccatgcaggg gatacttatt taagagtgaa ccctcaattt
     2821 gcagtgccta aaggaactga tgctttagtc agagtgtttg atgaatttca tggaaatgct
     2881 tatttagaaa ataactataa agttggtgaa atcaaattac cgattccgaa attaaaccaa
     2941 ggaacaacca gaacggccgg aaataaaatt cctgtaacct tcatggcaaa tgcttatttg
     3001 gacaatcaat cgacttatat tgtggaagta cctatcttgg aaaaagaaaa tcaaactgat
     3061 aaaccaagta ttctaccaca atttaaaagg aataaagcac aagaaaactt aaaacttgat
     3121 gaaaaggtag aagaaccaaa gactagtgag aaggtagaaa aagaaaaact ttctgaaact
     3181 gggaatagta ctagtaattc aacgttagaa gaagttccta cagtggatcc tgtacaagaa
     3241 aaagtagcaa aatttgctga aagttatggg atgaagctag aaaatgtctt gtttaatatg
     3301 gacggaacaa ttgaattata tttaccatcg ggagaagtca ttaaaaagaa tatggcagat
     3361 tttacaggag aagcacctca aggaaatggt gaaaataaac catctgaaaa tggaaaagta
     3421 tctactggaa cagttgagaa ccaaccaaca gaaaataaac cagcagattc tttaccagag
     3481 gcaccaaacg aaaaacctgt aaaaccagaa aactcaacgg ataatggaat gttgaatcca
     3541 gaagggaatg tggggagtga ccctatgtta gatccagcat tagaggaagc tccagcagta
     3601 gatcctgtac aagaaaaatt agaaaaattt acagctagtt acggattagg cttagatagt
     3661 gttatattca atatggatgg aacgattgaa ttaagattgc caagtggaga agtgataaaa
     3721 aagaatttat ctgatctcat agcgtaagga atagcagtag aaaaagtctg aatcaaaaat
     3781 gaagttctct caaaagttag aaataaaact ctgactttgg gagaatttca ttttattatt
     3841 aatatataaa atttcttgac atacaact
//
LOCUS       DAVKNL010000105         3227 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVKNL010000105 DAVKNL010000000
VERSION     DAVKNL010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.105"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(175..786)
                     /gene="rpsD"
                     /locus_tag="VW411_002053"
     CDS             complement(175..786)
                     /gene="rpsD"
                     /locus_tag="VW411_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU9794696.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(981..2033)
                     /locus_tag="VW411_002054"
     CDS             complement(981..2033)
                     /locus_tag="VW411_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU9794697.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2030..2728)
                     /locus_tag="VW411_002055"
     CDS             complement(2030..2728)
                     /locus_tag="VW411_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9794698.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(2895..>3227)
                     /locus_tag="VW411_002056"
     CDS             complement(2895..>3227)
                     /locus_tag="VW411_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9794699.1"
                     /translation="INVKETSDTANGSLSPSNSGSGVTPMNHNHATGTTDSMPADTMT
                     SSTNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGFLGLALAGLLGGLG
                     LKNKKEEN"
BASE COUNT          900 a          727 c          620 g          980 t
ORIGIN      
        1 aggattatac cacattgtgt actatttttg gttcatttta ctatatcact ttggactata
       61 tcggtctagt tatgtctcaa cgattatagc tggatactaa gagaaatcaa aaaagcacta
      121 agggagaacg tcccaaagtg cttaatatca aggcctatct tattcaataa aatattacaa
      181 cattttgttg tagaattcaa cgacaagtgc ttcgttgatt tctgggttga tttcgtcgcg
      241 ttctggcaag cgagtcaatg aaccttccaa tttttcagcg tcgaatgata cgaatgctgg
      301 acgtccaaga gtagcttcta ctgcttcaag gattgctgga actttcaatg atttttcacg
      361 aactgagatc acttgacctg gagttacgcg gtatgatggg atatcaacgc gtttcccgtc
      421 aacaaggatg tgaccgtggt ttacgaattg acgagcttga cgacgagtag tcgcgagacc
      481 aagacggtaa acaacgttat ccaaacgacg ttccaaaaga agcataaagt tgaaacctag
      541 gattccgcct ttgatttttg tagcttgtac gaacaagtta cggaattgtt tttcacctac
      601 accgtaagtg aaacgaagtt tttgtttttc agccaattgc aaaccgtatt ctgacaattt
      661 agaacggttg tttggtccgt gttgtcctgg tacgtagtta cgacgtgcca attctttacc
      721 tgtacctgta agtgaaaggc caaggcgacg agcttgtttc caagatggtc ctgtataacg
      781 tgacatatga atgtcctcct gatataaata atatttcggt ggaaatagtc acttagaaag
      841 ccctgattcg tgcagatgcc cttcgcctaa acagccaagg ttacttgtca taagacacct
      901 gttgacgagc ttcatgcttt cctgctgata tttcacacaa aatctattct accatgaata
      961 gctggatttg taaaggggtt ttaggcttta ttttcactac cagagaggtt gagaacgagg
     1021 gtaaaggtac ttcctagacc gtactggctg ctgactgtga tttccccacc caattgatgg
     1081 gccaattcac gcgcaatcgc aagtcctaat ccatgaccac ctgtcttcat gttacgcgaa
     1141 gtttcgacac gataaaggcg tttgaaaata ttttccaaat cctctggggc aataccctgc
     1201 ccttcatcgg tcacactgat tgaaagctgg tccttctcca gcttagccac cacttccagc
     1261 ttggttcctg gagcagaata tttaaaagcg ttatcgacca gattcaccaa gatacgagaa
     1321 agcttagcat aatctccctc aatccgggca gactctggga ttacctgcaa gtggacatct
     1381 cttctctcct gctcaatcaa aaactgaaat tcactcatgc actcaattaa gagcttgtcc
     1441 agaaaaatac tgtctttact ggtagtttcc acctgatttc tagctgtgtt tagggtcaaa
     1501 aaattcaact cctcaaccag tttattgagc ctctccgtct ggcgtccaat ggttgctaga
     1561 taatgagctt gctccgactc cttgataatc ccatccaaaa tcccttctac cgtcgcttgg
     1621 atcgaagtga taggagtctt aatatcatgc gacaactggg caatcatcaa gcccttttct
     1681 cgttcgcttt cttccaagga atcaaagctt acctgcaaat catgggacat ctcattaaaa
     1741 gtttgcccta attgctgaaa ttctacagga ccttgaacct ccaaatttga aggaaaatcc
     1801 ttggccgcta cccgcttggc atgctccttg agtttgccca acgacgtaaa gactggcaat
     1861 aggagaaaga gactaatccc agcaccgaca aggctggcaa cgatggtcat cccaagcaaa
     1921 aagtaaatct cgcctttcgc aatcagcatt ttttgaacag cccaaaaaac aaccaaaatg
     1981 gttaagaggg ttgaaataat atatccaacc aaaatataac tttttagttt catgtttgtc
     2041 ctctcggttt ctctatctta tatcccaacc cccaaactgt cttaatagtg ggagtttggt
     2101 cactactata ttttgccagc tcctgtcgaa gagcatggat atgcacattc aaggtattgg
     2161 tgtcatccac gtagtcttct ttccagatct tttcatagag gtctgtcttg gagaaaactc
     2221 gctctggatt actagctaaa atccacagca attcaaatga tttaacagtt aaatccagca
     2281 tttcttctcc tatttgaact tcatgactac tatgattcat ttttagattg ccaagggaaa
     2341 tcagctctgt ttcgccccca cgatgaaggc ggcgcaaaat attgtggaca cgcaaaacca
     2401 gctcacgtgg gctaaaaggc ttagcaataa aatcatctgc tcccaagctc aggccgtaaa
     2461 tcttgtcctg ttcactggtc ttagcagtaa taaataggaa aggctgctct ggtgataagt
     2521 attgaacctc actgattaaa tcataaccat ccatccgagg catcatgaca tctgtgataa
     2581 tcaaatcaat cggttttttc ttgaacagct ctagcgcttc cagtccatca tgggctacca
     2641 agacctgata acctgcctga attaagtatc tctgatgaat atctgtgatt tctacctcgt
     2701 cgtcaacgag taaaattgtc tttcccatct gtctctcctt tgataaaaac agtgctatac
     2761 tgctttaagt ataacactat ttttattgtt ttacgatgat tttaatctgc tattgaaaga
     2821 aagtcctgat tgctgattga gttgttaaac taatcttagt cagcccacta tccatcattt
     2881 atttccttag ctgattagtt ttcttctttt ttgtttttca aacctagacc accgagtaaa
     2941 cctgcaagcg caagcccaag gaaaccaata cttgccattg atgtttgagt ctcaccagta
     3001 tttggtagca tagctttatc ctctgacatc atcgtatcag acatcttgtt agcagaagca
     3061 gccatgtttt cacctgccat cgtgttggta gaacttgtca tggtgtcagc aggcatgcta
     3121 tctgtagtac ctgtagcatg attgtgattc atcggagtca cgccagaacc agagttagaa
     3181 ggtgataatg aaccatttgc tgtgtctgat gtttctttaa catttat
//
LOCUS       DAVKNL010000106         3197 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVKNL010000106 DAVKNL010000000
VERSION     DAVKNL010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.106"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(318..851)
                     /locus_tag="VW411_002057"
     CDS             complement(318..851)
                     /locus_tag="VW411_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEU9794700.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(940..1716)
                     /gene="recX"
                     /locus_tag="VW411_002058"
     CDS             complement(940..1716)
                     /gene="recX"
                     /locus_tag="VW411_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEU9794701.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDINENIVSQVIANLKEEKW
                     INDSQYAYAIINANQLSGDKGPYVLTQKLAQKGISKSTIEEILNDFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYFDAKIAFDELDSQVDEETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            1754..3109
                     /gene="rlmD"
                     /locus_tag="VW411_002059"
     CDS             1754..3109
                     /gene="rlmD"
                     /locus_tag="VW411_002059"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU9794702.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
BASE COUNT         1023 a          561 c          577 g         1036 t
ORIGIN      
        1 attgtactaa atgattgatg ctatgtggaa atacaaaaaa atgtttttga tacgaagttg
       61 acctgtattt tttatactaa tcattttcgt attttttgta ttaaacgata taagtttgtt
      121 gtaaacttac aaggaataaa gacattaaaa aataacagta tatctatttg ttttatatat
      181 tttacgaatt ctgcataaat ctctttctag taatgtgttg taactctgct ataatagatt
      241 tattcctttt tgtgtttaca caatttattt tatactacca aaaaaggtca ggattttgtt
      301 cctgaccttt gacaacttta ccgattcttt agttctacat agcgcttgta ccaaatgttt
      361 acataggctt ctgagaaagg accacgtcca ttgttaatcc aatcaacaag aattttgaca
      421 tgttctttta aaatatagtc caagtcatca gaataattca ttttgcgttt gtgacgctcg
      481 tactcttcaa cgtccaagag acgtttttcc ccatctgtaa aaattttaac atccaaatcg
      541 taatcaatat acttcagtgc ttcttcatcc agatagtagg ggctagccat attgcaatag
      601 taagaagttc cattatcacg aatcatggca atgatattaa accaatattt cttgtgaaag
      661 taaacaatag ccggttctcg agtgacccaa cgacgaccat cactttcggt aacaagtgta
      721 tgatcgttga caccaataat ggcgttttct gttgttttta gtaccatggt gtcccgccaa
      781 gttcggtgga gactcccatc atgcttataa ctttgaattg taataaagtc gccttctttt
      841 ggaagcttca taactaacca actttctaca atttataagt ttatcattta ctattgtacc
      901 ataaaattac ccaaaatctg tgaatttcac ttggaaatat taaagatatt ctctaagagc
      961 gcttgctata tccgaaaaat cgtagccctt tcgtgctaaa acttgagtta aacgctgctt
     1021 cagttcgtat ccttcatact ttcgggcata cttagtatat tgcttatcaa gttccttgaa
     1081 gatgagttcc tgagtcgttt cttcatcaac ttgactatcc aattcgtcaa aggcaatttt
     1141 agcatcaaaa taagagaagc ccttgttagt caagttctgg ataatcttat cttgcagggc
     1201 acgagctgga agttttccct catatttttt caatagttta ttggctacac gttgagcaac
     1261 ttccgaaaaa tcaaaatcat tcaagatttc ttctatagta gattttgaaa ttcctttttg
     1321 tgctaatttc tgagtcagta cataaggtcc cttgtctcct gaaagttgat tggcattgat
     1381 gatagcataa gcgtactggc tatcattaat ccacttctct tctttaagat tagcaatgac
     1441 ttgagaaacg atgttttcat taatatcata ttttttcaga tattctctga cctctttttc
     1501 agtacgtgct ttaaaggata agtggtagag ggccagattc ttaccataag aaaattgagc
     1561 aaagtcttga atctctttca attcctcttc gcttatcacc ttatctctcg ataacataaa
     1621 acgaacaatt gtgtcttcgg tgatatagca tttgtcgcca ttatcaagct ccatcagata
     1681 gagtcttttt ttcttttcaa gttttgtgat tttcatagct ctattataac tcaaaatgtg
     1741 ataagatagg ggtatgaatc tgaaagtgaa acaaaaaata ccattaaaaa tcaagcgcat
     1801 gggaattaac ggtgagggaa tcggctttta ccaaaaaaca ttagtctttg taccaggagc
     1861 tctcaaaggc gaagatatct attgtcagat tacttctatt agacgcaact ttgttgaagc
     1921 aaaattactg aaggtcaaca agaagtctaa atttcgaatt gtgccatctt gtactattta
     1981 taatgaatgc ggaggctgcc aaatcatgca cctgcattat gataagcagc tggagttcaa
     2041 gacggactta cttcatcaag cgctgaaaaa atttgctcct gcaggatatg aaaattatga
     2101 aattcgtcca actattggaa tgcaggaacc aaaatattac agagctaagt tacaatttca
     2161 gactcgaaaa tttaaaaatc aggtcaaggc gggcttatat gcacaaaact ctcactattt
     2221 agtagagttg aaagactgcc tggtacaaga taaggaaacc caagtgattg ctaatcgctt
     2281 agcagaatta cttacttatc accagattcc aatcacggat gagagaaaag ttctaggtgt
     2341 ccgtactatt atggtccgac gcgcgagaaa gaccggacag gttcagatta ttattgttac
     2401 aaaccgccag cttaatttaa ctcaattggt aaaagagttg gttaaagatt tcccagaagt
     2461 tgtgacagta gctgttaata caaatacagc taaaaccagt gagatatatg gtgaaaagac
     2521 agagattatc tgggggcaag agagtattca agaaggtgta ctcaattatg aattttcact
     2581 atcccctcga gctttttatc aactaaatcc tgagcaaaca gaagtcctct atagcgaagc
     2641 agtaaaagcg ctggatgttg ataaagaaga ccatttgatt gacgcttatt gtggagttgg
     2701 aacgattgga tttgcctttg caaagaaagt aaaaacactc agaggtatgg atattattcc
     2761 agaagctatt gaagatgcca agcgaaatgc taaaagaatg ggatttgaca atactcatta
     2821 tgaagctgga acggcagaag agattattcc tcgttggtac aaggaaggct accgagcaga
     2881 tgctttgatt gttgacccac cacgtacagg tctggatgat aagttattag atactattct
     2941 tacttatgta ccagaaaaaa tggtttatat ttcttgtaat gtttcgacct tggctcgtga
     3001 tttggtacgc ttagtagaag tctatgatct tcattatatc cagtcggtcg atatgttccc
     3061 acatacagct cgaactgaag ctgttgtaaa attaataaaa aaagtttaaa aaagtagttg
     3121 acaaagtttg aaaaggctgt ataatagtaa gagttgaaaa taacaactca ggtccgttgg
     3181 tcaaggggtt aagacac
//
LOCUS       DAVKNL010000107         3108 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVKNL010000107 DAVKNL010000000
VERSION     DAVKNL010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.107"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(195..398)
                     /gene="blpN"
                     /locus_tag="VW411_002060"
     CDS             complement(195..398)
                     /gene="blpN"
                     /locus_tag="VW411_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HEU9794703.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGRLLGP
                     WGAAIGGIGGAVVCGYLAYTATS"
     gene            complement(414..668)
                     /gene="blpM"
                     /locus_tag="VW411_002061"
     CDS             complement(414..668)
                     /gene="blpM"
                     /locus_tag="VW411_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="HEU9794704.1"
                     /translation="MDTKIMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGL
                     GTAICAASGVGAPFMGACGYIGAKFGVDLWAGVTGATGGF"
     gene            complement(818..1622)
                     /locus_tag="VW411_002062"
     CDS             complement(join(818..1252,1254..1622))
                     /locus_tag="VW411_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEU9794705.1"
                     /translation="MNYEASKQLTDARFKRLVGVQRTTFEEMLAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLQYVREYRTYEEIAADFGVHESNLLRRSQWVEVTLVQSGVTISR
                     TPLSSEDTVMIDATEVKINRPKKELANDSGKKKFHAMKTQAIVTSQGRIVSLDITVNY
                     CHDMKLFKMSRRNIGQAGKILADSGYQGLMKIYPQAQTSRKSSKLKPLTVEDKACNHA
                     LSKERSKVENIFAKVKTFKMFSTTYRSHRKRFGLRMNLSAGIINHELGF"
     gene            complement(1825..2229)
                     /locus_tag="VW411_002063"
     CDS             complement(1825..2229)
                     /locus_tag="VW411_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEU9794706.1"
                     /translation="MKTFLAKKRNIFLARLFLGQLPLLVSTYLFLSRQFLNFSVVFQF
                     LLVVINLASILVTVYLTREMRIREFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITA
                     GESYQQIIAYIGAVLCLIIMLLLMWGLKYYKK"
     gene            complement(2226..2390)
                     /locus_tag="VW411_002064"
     CDS             complement(2226..2390)
                     /locus_tag="VW411_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794707.1"
                     /translation="MKKKILIIFVLYLIMSIFLYPLRESAWYQLFYTIAYVIAVMIYF
                     AINKKKGEKK"
     gene            complement(2687..2806)
                     /locus_tag="VW411_002065"
     CDS             complement(2687..2806)
                     /locus_tag="VW411_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEU9794708.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            complement(2910..3059)
                     /gene="blpO"
                     /locus_tag="VW411_002066"
     CDS             complement(2910..3059)
                     /gene="blpO"
                     /locus_tag="VW411_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HEU9794709.1"
                     /translation="MDTKMMSQFAVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
BASE COUNT         1022 a          645 c          452 g          989 t
ORIGIN      
        1 aaaaaaacat aggcaataaa gagaaaaatt aatttatcat agattagaaa taatatgaca
       61 aaacaattca atgatgttaa ttcaatagtc ttttgttttt tatcggagat acttatggat
      121 agataaataa gataggtttg aaaagcgaag agaataataa agaatatagc cttcataaaa
      181 tttagctttc atttttatga tgtagcggta taggctaaat atccacaaac cactgctcct
      241 ccaattcctc ctattgcagc gccccatggt cctagaagtc tcccatattt cactccaccc
      301 gctgcacaac ctaaagcagc aactacagct gctcctccgg aattacctcc ataaacctca
      361 ctcagcattg tttcatttat attacaataa gtattcatac aagtctcctt ttattaaaat
      421 ccacccgttg cccctgttac tcctgcccaa agatccacac caaatttagc tcctatgtat
      481 ccacatgctc ccataaatgg tgctccaaca ccactcgcag cacaaatagc tgtccctagc
      541 ccccagccac caaaagcagc accaccacct tctaagacat tagtttgcca attattcttg
      601 cctccttcaa tactagataa catagttata tccatttcat gaaattgttc cataattttt
      661 gtatccatga caaatactct tttttatttt taatttttgt cttgttgtaa ctttgacaag
      721 tttagtatat catcgttttt taaaattttt catccagatt ttgaatagtc atcgaaacgt
      781 cttgaattgc aaaaattaca ttagacttcc tgcaaaacta gaatcctagt tcatgattga
      841 taataccagc actcaaattc attcgtaatc cgaagcgttt acgatgactt cgataggttg
      901 ttgaaaacat tttaaacgtt tttactttgg caaagatgtt ctcaaccttg cttctctcct
      961 tagatagcgc atggttacag gctttatctt caactgttag cggtttgagt ttgctggatt
     1021 tacgtgaagt ttgtgcttga ggatatatct tcatgagccc ttgataacca ctgtcagcca
     1081 agattttacc agcttgtccg atatttctgc gactcatttt gaacaacttc atatcatgac
     1141 aatagttcac agtgatatcc aaagaaacaa ttctcccttg acttgtgaca atcgcttgag
     1201 tcttcatagc gtgaaatttc tttttaccag aatcattcgc taattctttt tttagggcga
     1261 ttgattttta cttccgtcgc atcaatcatt accgtgtcct cagaactgag aggagttctt
     1321 gaaatcgtaa caccactttg aacaagagtt acttcaaccc attggctccg acggagtaag
     1381 ttgctttcgt gaacaccaaa atcagccgca atttcttcat aagtgcggta ttctcgcaca
     1441 tattgaagag tggccataag aaggtcttct aggcttaatt taggttttcg tccacctttt
     1501 gcgtgtttaa gttgataagc tgtttttaat acagctagca tctcttcaaa agtcgtgcgc
     1561 tgaacaccaa caagacgctt aaatcgtgca tcagttagtt gtttacttgc ttcataattc
     1621 atagaactat agtaaaatga aataagaaca ggataaatcg atcaggacag tcaaatcgat
     1681 ttctaacaat gttttagaag tagaggcgta ctattctagt ttcaatctac tatactatac
     1741 catattttgt ttcgcaggga atctattata aaagggtaag tattgcaaaa acacttaccc
     1801 ttttctttta tacttcatta agctctactt tttataatac ttcaagcccc acatgagcag
     1861 aagcatgatg attaagcaga gaacagcgcc aatataagcg attatttgtt ggtaggattc
     1921 tcctgctgtg atacctctat acaaacaaat aatagacata aaacctgtca agccgatgaa
     1981 cataagttga ttggttctag gactaaccaa atcatcatct tcaaactctc ttatcctcat
     2041 ttccctagtg agataaacag taaccaaaat agaagccaag ttaataacta ctaaaagaaa
     2101 ttggaaaact acggaaaaat ttaaaaactg acgagataga aatagataag tagaaacaag
     2161 caagggcaac tgacctaaga acaatctcgc aaggaagatg ttccgttttt tagcaagaaa
     2221 agttttcatt tcttttctcc tttcttttta ttgatagcaa aatagatcat aactgcaatc
     2281 acataggcta tggtataaaa tagctgatac caagcactct ccctaagcgg atatagaaag
     2341 atggacatga ttagatacag aacgaaaata atcagtattt ttttcttcat aagatttcct
     2401 cctaaatgtg cgatttatct tagttgagca agaacattta cactgctagt atagcactta
     2461 ttttgacctt ggatcactca aatcataaat ggtcatcaaa acctcttgaa ttgtaaaaat
     2521 taaaaaagca agcatgaaaa acatactttc ctctttatat tgtattgata ccaacttgtt
     2581 tgtagacttt tcatcctgct atcacatatc attttgacag gcgaaacaat attaaagaaa
     2641 ctcccctgta aattaagcta gcaaatacag gggagaaatt tattttttag agagtactat
     2701 ccgtatcctt tttggaagat tttgaaaata tttttctaat taagtcatcc atataaggac
     2761 caaatatacc aactactaaa ccaataataa aacttttaaa atccataatt accaccaaca
     2821 tattgctgca taggctacac ctccaagtat agctccacct gcagcaccag ttacacctat
     2881 tcctatagca aatggtccca atagaaatgt caaaccgttg ttgcacaccc atcaattgcg
     2941 ccatatgcaa cccctgctgc acaactaatt tttcttcccc aatcaatatc tccaccttca
     3001 acgcaagcaa gcatttcatt atccataact gcaaattgtg acatcatttt tgtatccata
     3061 tagtgtatca cttttcagtt acggaacaag tttaatataa aaattatc
//
LOCUS       DAVKNL010000108         3070 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVKNL010000108 DAVKNL010000000
VERSION     DAVKNL010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.108"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            <1..160
                     /locus_tag="VW411_002067"
     CDS             <1..160
                     /locus_tag="VW411_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9794710.1"
                     /translation="FAISSKGEKMKVENISYRVDHRKLFDNISFDTSSSDVTLITGKN
                     GTGKSTLL"
     gene            complement(526..1836)
                     /locus_tag="VW411_002068"
     CDS             complement(526..1836)
                     /locus_tag="VW411_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEU9794711.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            2073..2945
                     /locus_tag="VW411_002069"
     CDS             2073..2945
                     /locus_tag="VW411_002069"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEU9794712.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAILLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT          942 a          518 c          700 g          910 t
ORIGIN      
        1 ttttgctatt agttcaaaag gagaaaaaat gaaagtagaa aatatttcgt atagggtgga
       61 tcatcgtaaa ttgtttgata atatttcttt tgatacttcg agttcagacg tgacattaat
      121 tactggtaaa aatggtacag gaaagtcaac tttactatag tagattgaaa ctagaatagt
      181 acacatctac ttctaaaata ttgttagaaa tcgatttgac tatcctgatc tatttgtcct
      241 gttcttattt catttcacta tatctcaaat tgagtatgac gaagtgcgct cccatgtcct
      301 gggaacgcac tttcttcata tttttcatat tcttgaatcc atcgataaag actattggga
      361 tgaattttta aagttgaact aatcattttt acaggatgag atttacagca gagagtttga
      421 aggctttatc aaaggttttt cttggcataa tgacttttcc tcgtttccac ttaattttgt
      481 gtctacttta ttataccaag tccaccctta agttagataa taaatctaac ttaaggaagc
      541 tagaaggatg agaatccagg tggtcaagag tcccaaactt aagctgatgg ggacacccag
      601 aataatttgc tttttgaagg caaggccacg ttcctctata ttgggaagtg agagttgaat
      661 gagagaacca gctgatgaaa agggtgagat attagtagat agagcgccaa taacggtggc
      721 tgttgtgagt aagtgaatat caatctgagg attttgagca ctgatgatag caatgatggg
      781 aaagagggct ggagctacaa cggatagggt ggaactaaag agtgacatca ctccggctat
      841 cacacaaaag aacagaggta accagaaatg aggaatggtt gttgtcatga ggtgccctat
      901 cagtgtgact aaacctgact tgaccgctag agacattagt aagctcatgc cgcagagcat
      961 gataattgta gcccagggaa ccttagctaa aatggcttct tgcttcccta atttgagcct
     1021 taaggcgagg cagaccatga gtattgagac aaagccaata tcaaatgttt tttgataagt
     1081 agctatccag gcgatgtttg ggaaaatgag atgcaacaag ggaaaaagcc aaaccaaaac
     1141 catgctgctg atcatgagca aggtggtttg tctttgaacc ttgctgagga gtggtggttg
     1201 gtcaatagtc aaggatgagt ttgttcttcc cttactatag tgactgtaac aggataataa
     1261 aagcaagacg atgagtgggt agataatgct gacgataaag atatgattgc caagtgaaaa
     1321 agcttgctct tcccatccca tttgcttaaa caggccttga aagacaatgc ctgagctact
     1381 ggttatcaaa ttagcccctc ctgaagctcc ccaattgacg gcttgagctc caatcaaagg
     1441 gtgtttgtcc gctttttgac agagggtaat cgctagagga cagcaaacgg ccatagtagt
     1501 gaaaaatcca gcacctaaag cagacaaaag ggttgccatc aggtataaaa tcatgtagag
     1561 ggcgttaggg tgggtgcgtg tgcggtagag aatgtgttga gccaaaacat caagagtacc
     1621 gttagttgtt gcaacgttat aaaagagaga gacgctaaaa atggtaaaaa agagtgaggt
     1681 tggccaaaaa tgaagaagtt ctttggggct taatcccatg agagtggttg cgatgaggta
     1741 agaaaaagca atagccagca ggccaatatt gattttggtg cggtaaccaa ttccaatggc
     1801 tagagcaatg gcgctaatca ttattaaatg aatcattgaa ttgtcctttc agttagaata
     1861 tggaaatagg ttggcaattt tggcgctcaa aagaccacct gtttcctatt ggtacttgac
     1921 tattatacac aaaaaaaata caattgtctt gacaattgtc ttgacacttg ttagaattaa
     1981 aaatgaggta ttcaggcttg tgatttccta tggaagttaa tagtgattgc ctctaatgct
     2041 tacaagtgat attaaaaata gaggacctag tgatgtcaat catttcaact gatttaaccc
     2101 cttttcaaat agatgataca ttgaaagcag ccttgcgaga agatgttcat tccgaagatt
     2161 acagtaccaa tgccattttt gatcatcatg gccaagccaa ggtgtcgctt tttgccaagg
     2221 aagctggtgt tttagcgggg ctaaccgttt ttcaaagggt ttttacccta tttgatgccg
     2281 aggtgacctt ccagaatcct catcaattta aggatgggga tcgtttgact agtggcgatt
     2341 tggttttaga aatcataggc tcggtgagaa gtctcttaac atgtgaacgc gttgccttga
     2401 attttttaca acatttatca gggattgctt cgatgacagc tgcttatgta gaagccttag
     2461 gcgatgattg cattaaggta tttgatactc gaaaaactac tcctaattta cgtctttttg
     2521 agaaatatgc cgtgagagtt ggcggtggct ataatcatcg ctttaattta tcagatgcta
     2581 tcctgctaaa agacaatcac attgcggcag taggtagtgt tcaaaaggca attgctcaag
     2641 cgcgtgccta tgcccctttt gtgaaaatgg tcgaggtgga agtggaaagc cttgctgctg
     2701 ccgaagaagc tgcggcggcg ggtgctgata ttatcatgtt ggataatatg tcattggaac
     2761 agattgaaca ggccattacc ctaattgcag gacgttctcg gattgaatgt tctggaaata
     2821 ttgatatgac cactattagc cgttttcgtg gtttagcgat tgattacgtc tccagtggta
     2881 gtttaaccca tagtgctaag agtcttgatt tttccatgaa ggggttaacc taccttgatg
     2941 tctaagttgt aaaataaact aactttttaa aggatgtctt tcctctagaa cgagttttat
     3001 gtcagatagt ttaaacgcct cttcaaatat agtaaaatga accaaaaata gtacacaatg
     3061 tggtataatc
//
LOCUS       DAVKNL010000109         3020 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVKNL010000109 DAVKNL010000000
VERSION     DAVKNL010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.109"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            <1..1212
                     /gene="dcm"
                     /locus_tag="VW411_002070"
     CDS             <1..1212
                     /gene="dcm"
                     /locus_tag="VW411_002070"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEU9794713.1"
                     /translation="LDLFAGIGGFRMGMEAQGHECLGFCEIDKFARKSYKSIFQTEGE
                     IEFHDIRDVSDDEFKKLRGKVDVICGGFPCQAFSIAGRRLGFEDTRGTLFFEIARAAK
                     QIQPRFLFLENVKGLLNHDKGRTFTTILTTLDELGFDVEWQVLNSKDFGVPKNRERVF
                     IIGHSRKKGTRLLFPFRREGQATNSETLKALGNLNPSRSGMSGKVYYSEGLAPTLVRG
                     KGEGFKVAIPCMTPDRLDKRQNGRRFKENQEPMFTLNTQDRYGIVVVGDLPTSFKETG
                     RVYGSEGLSPTLTTMQGGDKIPKILIPEPIQFLKVREATKKGYAQAEIGDSINLERPS
                     SQYRRGRVGKGIANTLTTSGQMGVVVASYEGEDKQVYQVAGVLIDGQFYRLRIRRITP
                     KECFRLHGFPD"
     gene            1240..1350
                     /locus_tag="VW411_002071"
     CDS             1240..1350
                     /locus_tag="VW411_002071"
                     /inference="COORDINATES: protein motif:HMM:NF012372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEU9794714.1"
                     /translation="MNSQLYKQVGNSVTVPVIAAIAKKLKEIEEKDKSIK"
     gene            1334..1783
                     /locus_tag="VW411_002072"
                     /pseudo
     CDS             1334..1783
                     /locus_tag="VW411_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645893.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1776..2156
                     /locus_tag="VW411_002073"
     CDS             1776..2156
                     /locus_tag="VW411_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEU9794715.1"
                     /translation="MIDLYLSKNSQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIGLPFVVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            2169..2402
                     /locus_tag="VW411_002074"
     CDS             2169..2402
                     /locus_tag="VW411_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU9794716.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRHYKMEQRMPQSPWVERIVQILDLDYEDLFRR"
     gene            2405..2992
                     /locus_tag="VW411_002075"
     CDS             2405..2992
                     /locus_tag="VW411_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9794717.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQVLYGASLFS
                     FLWMYGQRKQVVKVNMKTRMKCLGIGLASLLIISLCFSLIHAQGSTNQTNLIGLQHQV
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALVHHPGTILAWCLYV
                     SLGMFLGMVRYKSDLWGSMGLHLVWNFLVYSLLLF"
BASE COUNT          989 a          451 c          641 g          939 t
ORIGIN      
        1 ttagatttat ttgctgggat aggaggcttt aggatgggaa tggaagctca agggcatgag
       61 tgcttgggat tttgtgagat tgataaattt gctaggaaat cttataagtc catttttcaa
      121 acggaaggag aaatagaatt tcatgacata cgagatgttt cagatgacga atttaaaaaa
      181 cttagaggga aagtcgatgt catctgtggg ggattccctt gtcaagcatt ttcaatcgca
      241 ggaagacgat tgggatttga agatactaga ggaactttgt tctttgaaat tgctcgggcg
      301 gccaaacaaa tccaaccacg ttttcttttt cttgaaaatg ttaaaggcct actcaatcac
      361 gataagggac ggacgttcac cacaatactt accacacttg atgagttggg gtttgatgtt
      421 gagtggcagg tgcttaacag taaggatttt ggcgttccca aaaacagaga gagggtgttt
      481 attatcggac attctagaaa gaaaggtacc agactcttat ttcctttcag acgagaaggt
      541 caagcaacta actctgagac tttgaaagca ctaggaaatt tgaatccatc aagaagtgga
      601 atgagcggta aagtctatta ttcagaaggt cttgcgccaa ccttagttcg tggtaaagga
      661 gaaggattta aagttgcgat tccttgtatg acaccagata gattagacaa gagacaaaat
      721 gggagacgct ttaaggaaaa tcaagagccg atgtttactt taaatactca ggatcgttat
      781 ggcattgtcg ttgttggaga tttaccgact agctttaagg agacaggtcg tgtctatgga
      841 agtgagggct tatctccaac actgactacg atgcaaggag gagataaaat ccccaaaata
      901 ctaattccag aaccaatcca atttctaaaa gtacgggaag caacgaaaaa aggatatgct
      961 caagcagaga ttggggattc aatcaattta gaaagaccaa gttctcagta tcgtcgtggt
     1021 agagttggaa aaggtatagc taatacgtta acaactagtg gtcaaatggg agtagtagtg
     1081 gctagctatg aaggagaaga taaacaagtt taccaagtag ctggtgtttt aatagatgga
     1141 caattttacc gtttgagaat acgacgaatc actcctaaag agtgttttcg attgcatggc
     1201 tttcctgatt aggcttttga agctgctaga aaagtctcta ttaatagtca gctctataaa
     1261 caagttggta atagtgtaac cgttcctgtg attgccgcaa tcgcaaagaa attaaaagaa
     1321 atagaggaaa aagataaaag cattaaataa agaatcaata ctagattgtg atgaattaga
     1381 aacagaatta catgatgcag aaatcaaaca gctggatgaa caaatatttt tgatgcccaa
     1441 ttatccatgt gagtttgagg taacattttt agatgattac cataaaaaac ataattaccc
     1501 actattttat gaatcctatc ttcaaaacgt tatggaattc cttgaaagtc aggatataaa
     1561 gaacggagtt aatgcctttg tagatgataa tcagaatctt gtttttgttt tatattgaca
     1621 agactatcga gccgagggaa aagaggggat acttacaacc caagtaactg taaaagctta
     1681 tgatgaagac aagaaatcga ttaacttctc aaagttgtta gattccttaa tcgtctcaga
     1741 atatcaaatg gaagcaaatt tgttggaggt ctcccatgat tgatctctat ctaagtaaaa
     1801 atagccaaag aaatcaactt cttttagact tcttccaaaa ctatggcatt gaggtatctt
     1861 gtcattcagt ttctgaaatg acaaaggaca aattaattga gatgatgagc tattcttcag
     1921 attgttttga gtttttatct cccaatttat tacgatttaa gaaccgtgac aatctaagat
     1981 taacggattt cgttgagata attttaaaaa atcctgaact aaccatcgga cttccttttg
     2041 tggtttcaaa taagcgggtt tatccaagtc tgaatttgga agaggctaga gctctattgc
     2101 caagagatac gaaacaattg atttacatgg cacaaacaca ttatttatct aactaaagga
     2161 gaagtaatat ggctgaacga ttttgggaga acttgtccat tattttggct gaaagaaata
     2221 tcagctggat tgagttaacc agaaaaatgt ttgcgggaga gtttcactat ccaagtgaat
     2281 tgaatcgttt atatcaaaag attcgccact acaagatgga acaacgaatg cctcaaagtc
     2341 catgggtaga aaggattgtg cagatattag atttagatta tgaagattta tttcggaggt
     2401 aactatgaaa agaataattc cagtttatat attccaacaa gtaaatgtcc tattggtatc
     2461 tctatactta ttgaaatttc tttgtatcgg tgagttaact atactacagg ttctctatgg
     2521 tgcgtctctt ttttcttttt tatggatgta tggtcaacga aaacaagtgg tcaaggtcaa
     2581 tatgaaaact aggatgaaat gccttggtat tggactagct agtctactga ttataagtct
     2641 atgttttagc ctgattcatg ctcaaggaag cacgaatcaa acaaatttaa ttggccttca
     2701 acatcaagtt ccttggtttt catttttatt gttcttaatc aatgcgagta tggttgaaga
     2761 atttctgtat cgtgaaattt tatggaactt ggttagaaaa ttagatattc gagttgcttt
     2821 gacaagtgtt ttatttgcct tagtacatca tccaggaacc attctagctt ggtgtctgta
     2881 cgtttcactt ggaatgtttt taggtatggt gcgctataaa tctgacttat ggggaagtat
     2941 ggggttacat ttggtgtgga actttttagt ttatagtttg ctgctttttt aaaataggtg
     3001 acctgattta tctcataaat
//
LOCUS       DAVKNL010000110         2811 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVKNL010000110 DAVKNL010000000
VERSION     DAVKNL010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.110"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            <1..1261
                     /locus_tag="VW411_002076"
     CDS             <1..1261
                     /locus_tag="VW411_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9794718.1"
                     /translation="EENYFRQIFEIISSILLFIVSLSFMLYLNFLVSIIFIVLSALPI
                     IVPVFMKKMLSNSANEYSNSNAEYTHIIKEIFNGFKTLKSYSVTKEIISLSDKKLDKL
                     EDSTFNLKRSEVLSKLVAVLISGFCFLVPLVVGCYFVIYHKSLSFSELIGIFLANDKV
                     LGPIQSIAYSLNKINTTKDLRKPFLKYLSGEKNFIDAEHDNNGLYTSSIDEIHMKDVV
                     YSITPENKLSIDFSFKSPFRVLLTGTSGSGKTTILNLINGSLKPQKGYVNLLSHGKKS
                     SDSIPTVDQTPYIFDTTIRENVTLFQNEYFSDDQIIEVLKKVNLYEELEKIDILNYQC
                     GENGSNLSGGQKQKIALARALIRNNKVYLFDEISANLDNDNSNSIHDILFNLGISFIE
                     VSHHYDLNDKRYTDIYKLENGTLFKIK"
     gene            1276..2016
                     /locus_tag="VW411_002077"
     CDS             1276..2016
                     /locus_tag="VW411_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9794719.1"
                     /translation="MDKDYILKVKGLYHQFLLGNNKTLQVLKNVSLSASRGEFISILG
                     ISGSGKSTLLKCISSLLEPTSGEVILNGINPYKIRNAKLSSIRRNEVSFIFQAYNLIP
                     SLPVIENIALPLRLSQKKLTIKNVENLLKRMKFNAGLNDFVGTLSGGEQQKVAIARAV
                     IADSDIIFADEPTGALDSVSREVIFELLRELVGAGKCVIMVTHDIELASKTDRALILK
                     DGKIFKELHRPSGEELYKILEVQSTTEE"
     gene            2019..>2811
                     /locus_tag="VW411_002078"
     CDS             2019..>2811
                     /locus_tag="VW411_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU9794720.1"
                     /translation="MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASRLQLSF
                     LVGGQIFIISSIISFIAYLCSIFVTSNYYYFLQYFFGENILPDIQFQTSAVGCIITVV
                     LISFLAFLSGCFYTFKKIRNRKSSKIRHVLSIVKRILLLAGFSVIWLLSLQQIFQDST
                     ILAKAQIIFNIVILDIVIIYQLSPFIQSCFIKLLSIIIFRNNFMFI"
BASE COUNT          967 a          355 c          493 g          996 t
ORIGIN      
        1 cgaagaaaac tattttagac aaatttttga aataatttct tcaatattgt tgttcatcgt
       61 ctctctaagt tttatgcttt atttaaactt cctagtttca ataatattta ttgtattatc
      121 agcattgccg attatagtac ctgtctttat gaagaagatg ttgtctaatt cagctaatga
      181 gtactctaac agcaatgcag agtatactca cataataaaa gaaattttta atggttttaa
      241 gacattaaaa tcttattctg ttactaaaga aataattagt ttgtcggata aaaagttgga
      301 taaactagaa gattctactt ttaatttgaa acgatcagag gttctttcaa aattggttgc
      361 agtactaatt tcaggttttt gctttctagt tcccttggtt gtaggatgtt attttgtaat
      421 ttatcataaa agcctttctt ttagtgaact gataggtatt ttcttagcaa acgataaagt
      481 tttagggccg atacaatcaa ttgcctattc attaaataag ataaatacaa ccaaagattt
      541 aaggaaaccg tttttaaaat acttaagtgg agagaagaat tttatagacg ctgaacatga
      601 taataacgga ctgtatactt catcaataga tgagatacac atgaaagatg ttgtatattc
      661 tattacacca gaaaataaat taagtattga cttctcattt aagtcaccat ttagggtatt
      721 attaacagga acttctggta gtgggaaaac aacgatttta aatttaatta atggttcttt
      781 aaagccacaa aaaggttatg taaatttgtt atcacatggg aaaaagagtt cagattcaat
      841 accaacagtt gatcagacac catatatttt tgacactact attcgtgaga acgtaacttt
      901 atttcaaaat gaatattttt cagatgatca gataattgag gtgttaaaaa aggtaaatct
      961 atatgaagaa ttagaaaaga tagatatact aaattatcaa tgtggtgaaa atggtagtaa
     1021 tttgtctgga ggtcaaaaac aaaaaatagc tttagctaga gctctgatta gaaataataa
     1081 agtgtactta tttgacgaaa tatcagctaa tttagataat gataattcaa attccataca
     1141 tgatattctg ttcaatttag gtatttcatt tattgaagtt tcacatcatt atgacttaaa
     1201 tgacaagaga tacactgata tatataaatt ggaaaatgga acacttttca aaattaagtg
     1261 attggaggca agaatatgga taaagattat atattaaaag tgaaagggct gtatcatcaa
     1321 tttttactag gaaataataa aacgttgcaa gtgctgaaaa atgtttctct ttctgcttcg
     1381 agaggagaat ttataagtat tctaggaatt agtggttctg gaaagtcaac tttattaaaa
     1441 tgtatttcta gtttgcttga accaacaagt ggggaagtaa ttttaaatgg aatcaacccc
     1501 tataaaatca gaaatgcaaa attgtcaagt ataagacgta acgaagtatc ttttatattt
     1561 caagcataca atttaatacc ttccctgccg gtaatagaaa atatagcact tcctttgcga
     1621 ttatcacaaa aaaaattaac tattaaaaat gtagaaaact tactcaaaag aatgaagttt
     1681 aatgctggct taaacgattt tgttggaact ctgtctggtg gagaacaaca gaaggttgct
     1741 atagctagag cggttattgc tgatagtgat ataatatttg ctgatgagcc aactggggct
     1801 ttagacagcg tttctcgtga agtaattttt gaattattga gagagttagt aggggcgggt
     1861 aagtgtgtaa ttatggtaac gcacgatata gaattggcct cgaaaactga tcgtgcatta
     1921 atattgaaag atggaaaaat tttcaaagaa cttcatagac ctagcgggga agagttgtat
     1981 aaaatcttag aggtacaatc aactacggag gaatagagat gctcagatta gtttactatc
     2041 aatttttaca taataagaaa caatggttag gcgtttcacc agtaattttt gtttcgagtt
     2101 tagttatggg attggctgtt aatggggtaa ttaatgttga gaataattcg caagtatttg
     2161 tcggacttcc agatcctaaa ccaattttta tgtttccgat agtattcgga ggagtaacat
     2221 tattctttgt gctctctaac ataataaata tgttggttga aatttttaga gatgattacg
     2281 aacttctgga ggtgttggga gcaagtcgcc ttcaactatc atttcttgtg gggggacaga
     2341 tttttatcat ttcctcgatt attagtttta ttgcatatct atgctctata tttgttacgt
     2401 caaattatta ttatttttta caatactttt tcggggaaaa tatattacct gatatccaat
     2461 ttcaaacgag tgctgttgga tgtatcatta cagtagtgtt gatttcattt ttagcctttc
     2521 taagtggttg cttttatacg ttcaagaaga ttagaaatag gaagagttct aaaataaggc
     2581 atgttctttc aatagtaaaa agaatactgc tattggctgg ttttagtgtt atctggttgc
     2641 tttctttgca acaaattttc caagatagca caattttagc gaaggcccag ataatattca
     2701 atattgtaat cttagatatt gtgattattt atcaattatc tccttttata cagtcatgtt
     2761 ttatcaaact actttctata attatattta gaaataattt tatgttcatt g
//
LOCUS       DAVKNL010000111         2786 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVKNL010000111 DAVKNL010000000
VERSION     DAVKNL010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.111"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            <1..710
                     /locus_tag="VW411_002079"
     CDS             <1..710
                     /locus_tag="VW411_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9794721.1"
                     /translation="IHQFGYTNHRIKLILSNEDLGATNARNIGIKNSRGKYISFLDDD
                     DEYMPDRILKLMACFKKSRMKNLALVCSYGIIIYPNGTREEEKTDFVGNPLFVQMVHN
                     IAGTSFWLCKKEVLELINGFEKIDSHQDGVVLLKLLVQGYQIDIVREFLVNYYAHSKE
                     NGITGVTQKTINADEEYYNYCRKYFNLLSFNERILVTKKYYSLNIKRLLLIGDKCKAL
                     KVIKKAREEKIFNEFLF"
     gene            797..2413
                     /locus_tag="VW411_002080"
     CDS             797..2413
                     /locus_tag="VW411_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9794722.1"
                     /translation="MYTIIKSNIKKFSLLTIFIVAGQLLLIYAATINALVLNELIAMN
                     LERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHS
                     KSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLM
                     IMLLVPKIFASKMREVSLNLTNQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAG
                     ILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTG
                     VIFTALGELGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYEAKNIC
                     YKYGDKEILKNLNFCFQRNEKYLILGESGSGKSTLLKLLNGFLRDYSGELRFCGDDIK
                     KTSYLNMVSNVLYVDQKAYLFEGTIRDNILLEENYTDEEILQSLEQVGLSVKDFPNNI
                     LDYYVGDDGRLLSGGQKQKITLARGLIRNKKIVLIDEGTSAIDRRTSLAIERKILDRE
                     DLTVIIVTHAPHPELKQYFTKIYQFPKDFI"
BASE COUNT         1004 a          319 c          510 g          953 t
ORIGIN      
        1 ttatccatca atttggctat accaatcata gaattaagtt aattttaagt aatgaagatt
       61 taggtgcaac taatgccaga aacataggta tcaaaaattc tagaggtaag tatatatcat
      121 ttttagacga tgatgatgaa tatatgccag atcgaatttt aaagttgatg gcttgtttta
      181 aaaagagtag aatgaagaat ttagctttag tttgtagtta tggaataata atttatccaa
      241 atggtacacg agaagaggag aagaccgatt ttgttggaaa tcccttgttt gttcaaatgg
      301 ttcacaatat agcaggtacg tcattttggt tgtgtaaaaa agaggtgcta gaattaatta
      361 atggttttga gaaaatagat tcacatcagg acggtgttgt tttattaaaa ctacttgttc
      421 aaggatacca aattgatata gtgcgagaat tcttggtgaa ttactacgct cacagtaaag
      481 aaaacggtat cactggagtg acacaaaaaa caattaatgc agatgaagaa tattataatt
      541 actgtaggaa atattttaat ttattgagtt tcaacgagag aatattggtt acaaagaaat
      601 attattcttt aaacataaag cggttactat taataggaga caaatgcaag gctttaaaag
      661 taatcaagaa ggcaagagaa gaaaaaattt ttaacgaatt tcttttttga aatatatgtt
      721 attatatcat tttggttttt tctattgtat atatgaaaac tatgttcaat taaaatttag
      781 aaagtgagaa actattgtgt atactattat aaaatcaaat ataaaaaaat ttagtttatt
      841 aacgatattt attgttgctg gtcaattatt gctaatttat gcagcaacta ttaatgctct
      901 ggtgttgaat gaattaattg cgatgaattt agagcggttt ttgaaattgt caatctacca
      961 aatgattgtc tggtgtggga taatattcct tgactgggta gtgaaaaatt atcaggttga
     1021 agtgatccaa gagtttaatc tagagattcg aaatagagtt gccacagaca tctctaactc
     1081 tacctatcaa gaatttcata gtaaatcatc aggaacatat ctttcgtggc taaataatga
     1141 tgttcagact ttaaatgatc aggcgtttaa acaacttttt ttagtaataa aaggaatttc
     1201 tggtactata tttgcagttg tgactcttaa tcactatcat tggtcattga ctgtagccac
     1261 cttgttttca ttaatgatta tgctacttgt accaaaaatc tttgcatcga aaatgcgaga
     1321 agttagtcta aatttaacta accaaaatga agctttttta aaatctagtg agactatatt
     1381 gaatggattt gatgtgttag cgtccttgaa tcttttatat gtattgccta agaaaattaa
     1441 agaagcagga attttattaa agatggttat acaaagaaag acaactgtag aaacgttagc
     1501 aggcgctatt agcttctttc tcaatatttt ttttcagata tctctcgttt ttttaacagg
     1561 ctatcttgca ataaaaggaa tagtgaaaat tggtactatt gaagcaatag gagcactaac
     1621 aggtgttatt tttacagcgc taggtgaatt aggaggtcaa ttatcctcta ttattggtac
     1681 gaagcctatt tttttaaaat tgtattcaat taatccaatt gagtcaaata aaatgaatga
     1741 tatcgaacca aatgaggtga atagagattt tccgttatat gaagcaaaaa atatttgcta
     1801 taagtatgga gataaagaaa tattaaaaaa cttaaatttt tgttttcaac gtaatgaaaa
     1861 gtatttaatt ttaggtgaaa gtggaagcgg gaaatctaca ttattaaaat tattgaatgg
     1921 ctttttgaga gattatagtg gagaattgcg attctgcggg gatgatataa aaaaaacctc
     1981 ctatttaaat atggtttcga atgttctata tgtagatcaa aaagcttatt tgtttgaagg
     2041 tacgattaga gataatattt tattggaaga aaattatact gatgaagaaa tactacagtc
     2101 tttagagcaa gttggtttga gtgtaaaaga ttttcctaat aacattttag attattatgt
     2161 tggtgatgat gggagattac tgtcaggagg gcagaaacaa aaaattactt tagctagagg
     2221 gctaattaga aataagaaaa tagtattaat tgacgaggga acttctgcta tcgataggag
     2281 aacttcgtta gcgattgaac gtaagatatt agatagagag gatttgactg tcattattgt
     2341 tacccatgct ccgcatccgg aacttaaaca atattttact aagatatatc aatttccaaa
     2401 ggattttatt taagaatata ggggagggaa acatgagatt ctattgtgaa gcttatgagg
     2461 taattactga agagaaaaaa gtatcatttt tttatgtaga tgagaaaccc ttaaggtatc
     2521 tgttatttga ctatatattt ctactagttc tagctaaaaa aaaatatccc aatcttatga
     2581 actgcacccc aaaagttaga tttattctgt ctaacttttg gggtgcagtt cattttgcat
     2641 agttttggca ttcatcctag atatccatat aaaatgtctg agattgttga aatgtctaaa
     2701 aaaatatcag atctaaacga taaaaaagaa tttcacaaaa agaactgtga gttgaggttt
     2761 caagatagtg ttcttgataa cataaa
//
LOCUS       DAVKNL010000112         2694 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVKNL010000112 DAVKNL010000000
VERSION     DAVKNL010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.112"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            96..344
                     /locus_tag="VW411_002081"
                     /pseudo
     CDS             96..344
                     /locus_tag="VW411_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            358..698
                     /locus_tag="VW411_002082"
                     /pseudo
     CDS             358..698
                     /locus_tag="VW411_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812122.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            691..1071
                     /locus_tag="VW411_002083"
     CDS             691..1071
                     /locus_tag="VW411_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEU9794723.1"
                     /translation="MIDLYLSKNRQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            1084..1227
                     /locus_tag="VW411_002084"
     CDS             1084..1227
                     /locus_tag="VW411_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794724.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRH"
     gene            1320..1907
                     /locus_tag="VW411_002085"
     CDS             1320..1907
                     /locus_tag="VW411_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9794725.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYGSSLIS
                     FLWMYGQRKQAHKVNMKSRMKWLGVEFVSLLIISLCFSLIHAQGSTNQANLIGLQHQI
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHTGTILAWCLYV
                     SLGMFLGMVRYKSDLWGSMGLHLVWNLLVYSLLLF"
     gene            2109..2246
                     /locus_tag="VW411_002086"
     CDS             2109..2246
                     /locus_tag="VW411_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HEU9794726.1"
                     /translation="MAEEVLNLQLVSVQVDETDEVDGMRFSTFSTNRCGNWSAFSWEN
                     C"
BASE COUNT          847 a          372 c          558 g          917 t
ORIGIN      
        1 aaagcaactc agttagttga tgaaatggaa caagtaggtt taatccgatt tgatgaattt
       61 ggaaatgttg gaattttagt cttggaggga caataatgaa gcgtattacc gctaatcaat
      121 atcagacatc agagcgttat tataagctcc ccaaactttt atttgagagt gaacggtata
      181 agaatatgaa actggaggtt aaggtggttt actcagtttt aaaagatcgg ttggagttgt
      241 ctttgagcaa aggttggatt gatgaggatg gggctattta tttgatttat tccaattcaa
      301 atttgatggc acttttaggc tgttcaaagt caaaattact ctccatgtga gtttgaagtg
      361 acatttttag atgattacca taaaaaacat aactacccac tattttacga atcctatctt
      421 caaaacgtta tggaattcct tgaaagtcaa gacataaaga atggggttga tgcctttgta
      481 gatgatcatc aaaatctcgt ttttgtttta tatggacaag gctatcgagc cgagggaaaa
      541 gagggaatac ttacaaccca agtaactgta aaagcttatg atgaagacaa gaaaccgatt
      601 aacttcgcaa atttattaga ttccttaatt tattagattc cttaatcgtg tcagaatatc
      661 aaatggaacc gaatctttgg gaggtctccc atgattgatc tctatctaag taaaaataga
      721 caaagaaatc aacttctttt agatttcttc caaaactatg gcatagaggt atcttgtcat
      781 tcagtttctg aaatgacaaa ggacaaatta attgagatga tgagctattc ttcagattgt
      841 tttgagtttt tatctcccaa tttattacga tttaagaacc gtgacaatct aagattaacg
      901 gatttcgttg agataatttt aaaaaatcct gaactaacca tcagacttcc tcttgcggtt
      961 tcaaataagc gagtttatcc aagtctgaat ttggaagagg ctagagctct attgccaaga
     1021 gatacgaaac aattgattta catggcgcaa acacattatt tatctaacta aaggagaagc
     1081 aatatggctg aacgattttg ggagaacttg tccattattt tggctgaaag aaatatcagc
     1141 tggattgagt taaccagaaa aatgtttgcg ggagagtttc actatccaag tgaattgaat
     1201 cgtttgtatc aaaagattcg ccactagaag atggaacaat gaatgcctca aagtccatgg
     1261 gtagaaagga ttgtgcaggt attagattta gattatgaag atttatttcg gaggtaacta
     1321 tgaaaagaat aattccagtt tatatattcc aacaagtaaa tgtcctattg gtatctctat
     1381 acttattgaa atttctttgt atcggtgagt taactatact acagattctc tatggttcgt
     1441 cactcatttc ttttttatgg atgtatggcc aacgcaaaca agcgcataag gtcaatatga
     1501 aatctaggat gaaatggctt ggtgttgaat ttgttagcct actgattata agtctatgtt
     1561 ttagtctgat tcatgctcaa ggaagcacga atcaagcaaa cttaattggc cttcaacatc
     1621 aaattccttg gttttcattt ttattgttct taatcaatgc gagtatggtt gaagaatttc
     1681 tgtatcgaga aattttatgg aatttggtca gaaaattaga tatcagagtt gctttgacaa
     1741 gtgttttatt tgccttagca catcatacag gaaccattct agcttggtgt ttgtatgttt
     1801 cacttgggat gtttttaggt atggtgcgct ataaatctga cttatggggc agtatgggac
     1861 tacatttggt gtggaaccta ctagtttata gtttgctgct tttttaaaat aggtgacctg
     1921 atttttaggt aacttctaaa actaacttcc agttttggga gagagatgga agttactttg
     1981 aaaagttacg gaaacattat tttctatgtg acaacattgt ataattgctt gcattttact
     2041 ggtggagaga gtataatgag ggtggataaa gtacttaatc ccaaaaattt aaataggagg
     2101 agctagctat ggcagaagaa gtattaaatc ttcaattagt atctgttcaa gttgacgaaa
     2161 cagatgaagt tgatggaatg cgatttagta cattcagtac aaatcgttgc ggtaactgga
     2221 gtgcttttag ttgggaaaat tgttaattca tttaaattgt aaactttaga gattctctgg
     2281 agttatggat gtcgttccaa tatgtgcacg ttggaatgtt agtgcttata tggggggaac
     2341 agaatcctct cttgattgaa gacaagctag tcattaggct ggtttgtctt tttgtcaact
     2401 gtagtgggtt gatataatag tattagtgag tgggataaaa gtttcattta gtttattcag
     2461 tacaaattta acgggtcaag atttatatac tagtggtgtt tttggggctg agagaagtat
     2521 cttgatttta tgtgtggttt ttatacttac agttgttctg ctccaaagag cttgtagaga
     2581 agaattagct cataaaggag attgattatt ttgatatcaa aaaaatgcac aggataacct
     2641 gatgcatttt tttagcgaca atgcttgcta cttccttctg tcgaatttag acaa
//
LOCUS       DAVKNL010000113         2683 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVKNL010000113 DAVKNL010000000
VERSION     DAVKNL010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.113"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            178..1188
                     /locus_tag="VW411_002087"
     CDS             178..1188
                     /locus_tag="VW411_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin formation protein"
                     /protein_id="HEU9794727.1"
                     /translation="MVINNIYDEYPIWSIPVNFKGSKWCLEQTGNDMYRGRAGILLFM
                     YYVKLLKDKSSYTQLYNKMLSAIPPSVSLSKTTFGLTGDIGYFIVLSIIEDYDTNTLA
                     CLNYIKSVLTELEKTDFASVSNKSDYINGLLPLINTLVRYYRRGIEKERVLRLVLSLG
                     DTLYNDVSERDNSNFGYSFGHGLSGVIFTLKNIYKVTRLKKYKELILQLVPREKIKIH
                     SLKWCSGEFGTLVNHPQSVPEGIVASLDNDTFCHGSMAIINFYIDYMKEMDTSMELES
                     LLCSVVMNKSKEGEYRIVQTSNFPDFSLYTGVTGIAYTFLRYIALNQNDKKIVIPSLL
                     EI"
     gene            1538..2242
                     /locus_tag="VW411_002088"
     CDS             1538..2242
                     /locus_tag="VW411_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEU9794728.1"
                     /translation="MNKIFIYAGVRNHNSKTLEYTKRLSSIISSRNNVDISFRTPFNS
                     ELEISNSDSEELFKKGIDRQSNADDGGVIKKELLESDIIIISSPVYLQNVSVDTKNFI
                     ERIGGWSHLFRLAGKFVVTLDVAESNGSDNVSEYLRDIFSYMGGQILHQVSITNSLKD
                     IAEAQLMEATYKIEDVLEGKIKYKTTDYQERAYQTLKLILENYDSEHFEKMYWEKKRL
                     FEANSLEEWYYVENIK"
BASE COUNT          955 a          319 c          468 g          941 t
ORIGIN      
        1 ttacctaata ataatttgga aaatttagaa aagcaacttg atatcattga aatttatttt
       61 ccaagctttg atcagcgaaa aaaaggatat aaattaaact tatctaatag aaaaaaactc
      121 ccaaatgaaa gtttagttac tattctgcat aatgaagtag aaaatattgc agactctttg
      181 gttattaata atatttatga cgaatatcca atttggagta tacctgttaa ttttaaaggt
      241 tctaaatggt gtcttgaaca aacgggtaat gatatgtata ggggtagagc tggaatatta
      301 ctgtttatgt actatgtaaa attattgaaa gataaatcat catatactca actgtacaat
      361 aaaatgttat ctgcaattcc gccatctgtt tctttatcta agaccacatt tggtttaact
      421 ggggatatag gatatttcat tgttctatcc attatagagg attacgatac aaatactctc
      481 gcatgcttaa attatataaa gagtgtttta actgagcttg aaaagactga ttttgcaagt
      541 gtttcaaaca agtctgacta tattaatggt cttctacctt taattaatac actggtaaga
      601 tattatagga gaggaataga gaaggaacgt gttctaaggc tagtattaag tttaggtgat
      661 acgctatata atgatgtaag cgagagagat aatagtaatt ttggatatag ttttggccat
      721 ggactatcag gtgtaatttt tacattaaaa aatatttata aagtgacaag acttaaaaaa
      781 tataaagaat taattttaca actagttcct agggagaaaa tcaaaattca ttctttgaag
      841 tggtgtagtg gagaatttgg gacattagtt aatcatcccc aaagtgttcc agagggaatt
      901 gtagctagtt tagataatga tacattttgc catggaagta tggccataat aaatttttac
      961 atcgactaca tgaaagaaat ggatactagt atggaattgg agtctttatt gtgtagtgtt
     1021 gtaatgaata aatcaaaaga aggagagtat cgtatcgtcc aaacaagcaa tttcccagat
     1081 ttttctttgt atacaggtgt gactggtatt gcttatactt ttttaaggta tatcgcactg
     1141 aatcagaatg acaaaaagat agttatcccc tcattacttg aaatctagct taatttatag
     1201 ttttgtttct aaaatagagg tgtccactca ttaagcaata taaaaatacc aagattgact
     1261 agtttaatat gtaattctat aaaatatttt taatagtgta gcttttcgtt gagataataa
     1321 tataataaag aaaagttcta ttctaattgc taaacggctt gaggtatatg ctactgttat
     1381 agaaatgatt ttaaactaaa atcaaaatta ctccaaaaat agtattgatt ttggtttaat
     1441 acaaaacata tgtaataatg tgagtcaaca aaagtctgtt tttgtaccat actatcttga
     1501 aaaatctact aaattcaaat tgagaggatg aattaaaatg aataaaatat ttatatatgc
     1561 aggagtgcga aatcataact ctaagacttt agagtatact aagagacttt caagtatcat
     1621 ttctagtaga aataatgttg atatatcttt caggacacct ttcaattcag agctagaaat
     1681 atctaattct gattcagaag agttgtttaa gaaaggaatt gataggcaaa gtaatgctga
     1741 tgatggaggt gttataaaaa aagagttatt agagtctgat attataatta tctcttctcc
     1801 tgtatattta caaaacgtat ctgtggacac aaaaaacttt attgaaagga ttggggggtg
     1861 gagccatctt tttcgattag ctggaaagtt tgttgtaaca ttagatgttg ctgagagtaa
     1921 cggctctgat aatgtgtcag aatatcttag agatatattt tcatatatgg gaggacagat
     1981 attacaccaa gtttcaatta ccaattcact caaagatatt gcagaggctc agttgatgga
     2041 ggcaacttat aaaattgaag atgtacttga agggaaaatt aagtacaaaa caacggatta
     2101 tcaagaaaga gcttaccaga cattaaaatt aattttagaa aattatgatt ccgaacattt
     2161 tgagaagatg tattgggaga aaaaacgcct ttttgaagct aatagtttag aggaatggta
     2221 ttatgttgaa aatattaaat aactctgtca tatatttatg tttgattccg ttattgttcc
     2281 tattattgat aatttttcca aatgattcat taatctatta ttttcgattg attttaattt
     2341 cgttaatatc aattacatta catgaactag gtcatttttt agtgggaaga tgtttatctt
     2401 ataaactaga gatgcttgct actccctttt tcttctattt cagaaaaaaa atatatttca
     2461 aatttccagt gttattagca ttcggttatt gtcaaatgag taatagaaac attactaatg
     2521 aaaaaaatag tgataggaat ttggtgttct acttctttgg gggaggtgga gctaacctaa
     2581 ttgtcgcaat attagcactt ttgggatttg tgccgtacgc atcagaattt tttattttga
     2641 atataattct ttttctagtt acggtatgtt taccaattga tgg
//
LOCUS       DAVKNL010000114         2594 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVKNL010000114 DAVKNL010000000
VERSION     DAVKNL010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.114"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            <1..1234
                     /locus_tag="VW411_002089"
     CDS             <1..1234
                     /locus_tag="VW411_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9794729.1"
                     /translation="KPVADILKNNTSSYTRYKKEMVIDNVKVDDKVCEIISEIMDESY
                     TDIQILTHKLYSKGDDIKASKTIWMKKSGKEYSEAFAGTGEARIILLVNDIVNAQSNS
                     LILIDEPEISLHPSAIYKFKEFLLQECLNKKHQIIITTHSTQLIKDFPREAVKLLVKN
                     GEKVDVIENIDYQDAFFELGDVYHSRKMIYVEDRLAKYILEFVITHSGSENLKQNLVV
                     RYIPGGANQIICNNILNSSYLDSDNHYFWLDGDQNTNVSESNNLMNYLENGVVISDKI
                     PESDNKNLDDIIKLITGCPIKFNVSGNKGQKNNIELIAKQRSFIDYWAKYVSYLPFPT
                     PEFFLANLCNSVDREGYDFSKDGNGKEYFRKKTQVALGIENITSEDIFQEQRRAVSKI
                     QPESSMFQCIKEKLEALF"
     gene            1614..2417
                     /locus_tag="VW411_002090"
     CDS             1614..2417
                     /locus_tag="VW411_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEU9794730.1"
                     /translation="MQPTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPAILLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADMVKKIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNTPFQQHSKY
                     FRDALVLDNAKILQRRPEFLTAFFENLLLGGQNDLSSEKMYLDLDLDLS"
BASE COUNT          904 a          371 c          472 g          847 t
ORIGIN      
        1 taagccagtt gctgatattt taaagaataa tacaagtagc tatacgcgtt ataaaaagga
       61 aatggttata gataatgtta aagttgatga taaagtttgt gaaattatta gtgaaataat
      121 ggatgaatct tatacagaca ttcaaatttt aacacataag ttgtattcaa aaggtgatga
      181 tataaaagct tccaaaacta tttggatgaa aaaatctgga aaagaatatt ccgaggcttt
      241 tgcaggaaca ggtgaggcta gaattatttt gttagtaaat gatatagtaa atgcgcaatc
      301 aaattcatta atattaatag atgaacctga aatctcactt catccgagtg caatctataa
      361 atttaaagag tttttacttc aagagtgttt aaataaaaaa catcaaatta ttatcactac
      421 acattctaca caacttataa aagattttcc tagagaagcc gtgaaacttt tagtgaaaaa
      481 cggagaaaag gtagatgtta ttgaaaatat tgattatcag gatgcatttt ttgaattagg
      541 tgatgtgtat cattctagga agatgattta tgttgaagat agactagcta aatatattct
      601 agagtttgtt atcactcatt caggtagtga gaatcttaaa cagaatttag tagtgagata
      661 tattcctggt ggagcaaatc aaataatttg taataatatt ttaaactcat cgtatttaga
      721 ttccgataac cattattttt ggcttgatgg agatcaaaac actaatgtta gtgaatcaaa
      781 taatttaatg aactatcttg aaaatggtgt tgttatatca gataaaattc ctgaatcaga
      841 taataaaaat cttgatgata ttataaaatt gataacggga tgtccaatta aatttaatgt
      901 ttcaggtaat aaagggcaaa aaaataatat tgaattaatt gcgaaacaaa gaagctttat
      961 agattattgg gctaaatacg tttcttatct tcctttccca accccagagt tttttttagc
     1021 taatttgtgt aacagcgtag atagggaagg ttatgatttt tctaaagatg gcaacggaaa
     1081 agaatatttt agaaaaaaaa ctcaagtagc gttaggaata gagaatataa cttctgaaga
     1141 tattttccag gagcaaagaa gagctgtttc aaaaatacag ccagaatcat caatgtttca
     1201 gtgtattaaa gaaaaattag aggcattgtt ctaattcctt agattaggta ctacataaca
     1261 aattttttaa attcaaaccc gcaccaaaaa caacattgca actttcttct caggtaatgt
     1321 gttgggattt gaacagacca acgagttcat agtggtgtat gccaatgact ttgataagtc
     1381 tacgcgtcag acttattagt tcatttatcc agatatatct cgataatcat attatttagt
     1441 caaagaacat cccatatgag ggcatgaaat gctaagttta gcttgctcac tattaagatg
     1501 aagtatatat atttcataga acctattgtg aaggattttt ataattttcg ccaattccat
     1561 acctatttca caatttaccg atgacacaga aaaacaattg aaaggatatt tctatgcaac
     1621 caacttacaa cattgacaat ccaaacttgt cttatgaagc gaagcgtgat ttatggcgga
     1681 taggttttgg tctgcagaaa gttgacaatc tagtgccatc agcgtatatg gaatctttgg
     1741 ctgagaaaca gtcccggggt gaactgactt atgagcaggt ttatgaggat gcaacggctt
     1801 atcatcatac cattgatgcg agtacagagg aggcagactt ggtttctcta cgtattgtag
     1861 aactattgtc tcgaagaggc tttagcttca gtcctgcgat cttacttgct attcataagg
     1921 agttgtttca agatatattt gaaccctcga ttccggtagg tcaatttcgt cagactaata
     1981 tcacaaagaa tgaacctgtt ttgaatggtg aaagtgttgt gtactctgat tactccatga
     2041 ttcaaatgac cttggattat gattttaatc aggaaaaaca agttgcatat gcgacactaa
     2101 cccaggcgga tatggttaaa aaaatccagc attttatttc aggaatctgg cagattcatc
     2161 catttcgcga aggaaacact cggacggtaa cggtattttt gattcagtat cttcgtgagt
     2221 ttggttttga tattgataat acaccatttc agcaacattc caagtatttt cgtgatgcct
     2281 tagtgttaga taatgcaaag attttacagc gacgtcctga gtttttaaca gctttttttg
     2341 aaaatctctt gctcggtggt caaaatgatt tgtcttcaga aaaaatgtat ctagatttag
     2401 acctcgatct ttcataatcc taatactgag taaacattga attttaggaa aaaatgaagt
     2461 aaatattctc acaagaaaac gtatatcatc aaagtttggc tctttgtcaa ttgtagtggg
     2521 ttgaagaaaa gctaagttcg agaaaggaca aatttcgtcc tttctttttt gatgttcaga
     2581 gcgataaaaa tccg
//
LOCUS       DAVKNL010000115         2188 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVKNL010000115 DAVKNL010000000
VERSION     DAVKNL010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.115"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            9..1292
                     /locus_tag="VW411_002091"
     CDS             9..1292
                     /locus_tag="VW411_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9794731.1"
                     /translation="MVEIVRGGSPRPIKDYLTSEVDGINWIKIGDTEKGEKYINNVKE
                     KIKKSGLNKTRFVKKGTFLLTNSMSFGRPYILNVDGAIHDGWLAISNYENSLNKDYLF
                     YILSSNVVYSQFLSLISGAVVKNLNSDKVASILIPLPPLSEQQRIVEAIESALEKVDE
                     YAESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFE
                     EGKIKKKDLDISIVSQGDDNSYYEEVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIP
                     IYPVIAQKCNQWSGFSIDLARFIDPETVHSYQKERLLRDGDLMWNSTGLGTLGRLAIY
                     HENKNPYGWAVADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELL
                     TKTIKEYLIPLPPLPEQSRIVDKIEQFFAHIDALI"
     gene            1349..2146
                     /gene="psrA"
                     /locus_tag="VW411_002092"
     CDS             1349..2146
                     /gene="psrA"
                     /locus_tag="VW411_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HEU9794732.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
BASE COUNT          790 a          309 c          389 g          700 t
ORIGIN      
        1 tttctacatt ggttgaaatt gtcagaggtg gctctccacg accaatcaaa gattatctta
       61 cttctgaagt agatggaata aattggataa aaataggtga tactgaaaag ggtgaaaagt
      121 atataaataa tgttaaagaa aaaatcaaaa aatcagggct taacaaaact agatttgtaa
      181 aaaaaggtac atttttgtta actaattcta tgagttttgg tagaccttat attttgaatg
      241 ttgatggtgc aatacacgat ggatggttgg ctatttcgaa ctatgaaaac tcattaaata
      301 aagattacct attctatatt ctttcatcaa atgtagttta ttctcaattt ctatctctaa
      361 ttagtggagc tgttgtgaaa aacttgaata gtgataaagt tgcttctatt cttatccctc
      421 tccccccact atccgaacaa caacgaatag tagaagcaat cgaatcagct ttagaaaaag
      481 tagatgaata tgctgaaagt tataatagac tagaacagct agataaagaa tttccagata
      541 aactaaaaaa atctattctt caatatgcta tgcaaggaaa attagttgaa caagacccaa
      601 atgatgaatc agtcgaagtt ttacttgaaa aaatacgagc agaaaaacaa aaactctttg
      661 aagaaggcaa gattaaaaag aaagatttgg acatttctat tgtttcccaa ggagatgata
      721 actcttatta tgaggaagta ccttgtgaaa taccagaaag ttgggaatgg gtgaggttaa
      781 acgatataac ctcctacatt caaagaggga agtctccaaa atattcaaat attcctatat
      841 accctgtgat tgctcagaaa tgtaatcaat ggtctggctt ttctattgac ttagcaagat
      901 ttattgatcc cgaaacagtt catagttacc agaaagaaag attactaaga gatggtgatt
      961 taatgtggaa ttcaacaggt ctaggtacgt taggacgatt agctatttac catgaaaata
     1021 aaaatcctta tggctgggct gttgcagata gccatgttac agttataaga gtcttatcgg
     1081 gggttattaa ttgccatttc atctataatt ttctttcctc tcctattgtt cagtcagtta
     1141 ttgaagaaaa agcgtcgggt agtacaaaac aaaaagaatt attaacgaaa actataaaag
     1201 aatatttaat ccccctccca ccccttcctg aacaatccag aatcgttgat aaaatcgaac
     1261 aatttttcgc ccatattgac gcactaattt agtataatag ttttacgtgc ggtggaattt
     1321 ctatcaaaca taagatagga gttttacgtt gaaagatttt gaattacatt tgaagaaagc
     1381 tggtttagct gaaaatactg tgcgttcgta cctttatggt gttcgatttt tcttggagaa
     1441 ttacgaactc aaaatggaag atttatttga atacaaagga tatttattag acaattttaa
     1501 acctaaaact gtcaatttgc gactacaagg agtaaataaa tatctggctt ttattggtca
     1561 tgatgatttg aaattaaaat ttgtcaaagt acagcaaaaa ccgtttttag aagatgtcat
     1621 tagccatgct gattaccttt ttcttaaacg tagtttgaaa aaagatggta ttttaaaatg
     1681 gcattttgtc gtttggtttt taggtgcaac tggggctcgt gtaagtgaac ttattaaact
     1741 aaaagtagag catgtagaaa tcggttattt tgacatttac tcaaaaggtg gtaaaattcg
     1801 tagactgtac attcctaaaa aattaagaaa tagttgtctt agttggttag aatcagaaaa
     1861 tcgccgaagt ggttatttat ttttgaataa atttaacgaa ccaattacag caagaggagt
     1921 tgctcaacag ttaaaaaatt atgctgataa atacaaaatg aatcctaaag taatttaccc
     1981 tcattctttt aggcatttat ttgctaagaa ttttttagcg aagtataatg atattgcctt
     2041 gcttgcagat ttgatgggac acgaaagtat agaaactact cgaatttatc taaggaaaac
     2101 agctactgaa caacaaaata ttgtagataa aattgttaat tggtaaaaaa taacaggtgg
     2161 tcaaactgac tacctgctat ttttgtga
//
LOCUS       DAVKNL010000116         2134 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVKNL010000116 DAVKNL010000000
VERSION     DAVKNL010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.116"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(418..1908)
                     /gene="lysS"
                     /locus_tag="VW411_002093"
     CDS             complement(418..1908)
                     /gene="lysS"
                     /locus_tag="VW411_002093"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEU9794733.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLITKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(2036..>2134)
                     /locus_tag="VW411_002094"
     CDS             complement(2036..>2134)
                     /locus_tag="VW411_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HEU9794734.1"
                     /translation="TYDLEPMAQQVIPKAAFGYIASGAGDTFTSFQ"
BASE COUNT          614 a          433 c          424 g          663 t
ORIGIN      
        1 ttatttgctg attctatcta ctaatactat aaaaaacaag tgaataagac accagaacat
       61 cctctaatag gacctatgga cttatatcaa tcccatatat actgtttaag caagatacta
      121 tcctagttga cgctaacttc ctatcccact tattaatttt ttatttatag tgaatctgaa
      181 caaatcaatt ggggaatcca agtcaatttc tagtactttt tgaaaagttt cagcgtacta
      241 tcgtaaaatg aaataaaaca tgcacaaatt gcttagggaa tttaaaccaa tttctaacaa
      301 tgtcttagaa atcaaagtgt actatttcaa tttcaatata ctacacttca ttttctcctt
      361 aaattcagtc tctttttgaa tcaaaaaatc ctctgataag acccagagga taagaattta
      421 tttcattgtt gggaagagca atacatcacg gatagttgtt gtatcagtga ggagcatgca
      481 gagacggtcg ataccgattc ccaaaccacc tgttggtggc ataccgtatt caagagcttc
      541 aatgtagtca tagtcgattc ctgtcgcttc atcatcacca agttctttgg ctttagcttg
      601 ggcttcaaaa cggctaagtt ggtcgattgg gtcgttcaac tcagtaaagg cattaccgta
      661 ctccttagtc atgataaaga gctcgaaacg gtcagtaaag cgttggtctt caggattttt
      721 cttagcgagt ggagatacag ctactggatg tccatagaca aaggttggtt ggattaaagt
      781 ttcttcaaca aactcttcaa agaaggcatt gatgatgtga ccaacctcag tgtagtgttt
      841 ctcaactgga actttcttct cagcagcgat agctttagct tcttccaaag tcatgtcttg
      901 ccagaaatcg acaccagtaa tttctctgat agcatccacc atatgaacac gcttaaatgg
      961 ttcgttaatc ttgatttcag taccttggta gttgactggg ccatcacctt tgactgattt
     1021 agcagcgtgt tggataatgc cttcagtcaa gtccatgatg tcttggaagt ctgcataagc
     1081 ttggtaaact tcgatagaag tgaactcagg gttatgagta gcgtccattc cttcgttacg
     1141 gaagatacgg ccaatttcat agacacgttc cataccaccc acgataaggc gttttaagtg
     1201 aagctcagtc gcgatacgaa gcaccatgtc aatgttttgg gcattgtggt gggtgataaa
     1261 tggacgggca gcagcaccac cggcttcatt atgaagaaca ggtgtttcca cttcaaggaa
     1321 tcctttttgg tcaaggtaac gacggatttc agagatgatt tttgaacgag tgacaaagcg
     1381 ttcaaagctt tcacgattag aaatcaagtc aaggtaacgt ttacggtaaa ttgtttcaac
     1441 gtctgtcaaa ccatggaatt tctcaggaag aggacgaaga gccttagaca agtgtgtgat
     1501 gtgggttgcc ttgatagaga gttctcccat atccgtacgc atcacttcac cttcgacacc
     1561 aaggaagtca ccaaggtctg cttttttgaa gatttcgtag ttttcttcac cgacagcatc
     1621 cttacgaacg tagatctgaa tctggccttc gcggtcttga aggtgggcaa aaccaacttt
     1681 tcctttacca cgtttggtta tcaagcgtcc tgcgatagta gctgtttcgt ttttatcgtg
     1741 taattgttct ttatcgaggt tggcatattt atcttttaat tcttgtgaat ttgcagtacg
     1801 ttcaaaacgt tttccgaaag gatcgattcc ttgttcgcgg agcgcagcca ttttttcacg
     1861 gcgaacgatc tgctggtcat ttagttcttc catatgttct gtagacatgg gatcctccta
     1921 gttttactca aaattgaata cgatgagtca ttttttgcga cactcccatt ttatcataaa
     1981 taagcaagaa attcacggat aatctttaaa aactaaaaag aacctgacgc taaaatcact
     2041 ggaaagaagt gaaagtatct cccgccccac tagcgatata gccaaatgct gctttaggaa
     2101 taacttgttg cgccattggc tccaaatcat aggt
//
LOCUS       DAVKNL010000117         1978 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVKNL010000117 DAVKNL010000000
VERSION     DAVKNL010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.117"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            714..998
                     /locus_tag="VW411_002095"
     CDS             714..998
                     /locus_tag="VW411_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794735.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            991..1140
                     /locus_tag="VW411_002096"
     CDS             991..1140
                     /locus_tag="VW411_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794736.1"
                     /translation="MDKMKPVFQALNKELIQENLTLTIICVGGYVLEYHGLRATQDVD
                     AFMAL"
     gene            1255..1978
                     /locus_tag="VW411_002097"
                     /pseudo
     CDS             1255..1978
                     /locus_tag="VW411_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436408.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          704 a          365 c          312 g          597 t
ORIGIN      
        1 aataaagtta caaagatgta cgaccacact gttgtaaatc atagtgttcg cgaatatatt
       61 actgatagca tttctacaaa tacaagtaaa gagagcggat gagattcaaa cgaaatatgt
      121 cagtgctttg gcattcctag ccttcatatc atttaaagaa ttctatagac aaaatttttt
      181 ccaatacaga cactcgtaac aactgcttca tttttctacc aacatattta ggaacaggat
      241 aagatacaag agtattaatc catagctcag ttctatacca atctaagaca aataagctaa
      301 aaaaacgatt gataataagc aaatagattc caaattttct ctatctgctc attttaataa
      361 acaatactag tgtaactatc cttccagtca gaagcttgtc aaatcacacc gaaaattctt
      421 ctaaaattta tctcgttagg caatcaagca aaaactcgac gatagtacaa acattatcat
      481 acaggattga cttcctaaat tatatacttt agtaaggttt tcggataaga aaaaaggttc
      541 attttacatt tctaaacatt cttttctaag atgaaaaaca gaatttttcg attgtgattt
      601 aaagcaacaa gaagattttc agtatcatcc tatagatacg agctaattaa gaaaaactac
      661 atttttgaat ataaactaca ataatataaa ctaaatttta taggaggaag acaatggatt
      721 ggtacgatta tatgatacag gcatccaaac aatcacaatt caacgcaagc cattggtttc
      781 gctatttgcg aaaagttatt tttgaagact attcttattt aacaaaccaa gatgtagaaa
      841 agttgctaga ctccaaagaa ctaacccgtt ttcaaaaaat tagcttgaag tatgcctttc
      901 aagagcatac tccaactcat aaatatgtga tttcattaaa taaacctgct aagttaacca
      961 atgttcaaaa attgatggag aaatacaaac atggataaaa tgaaaccggt cttccaagcc
     1021 ctaaataagg aattaattca ggaaaatctg actttaacaa ttatctgtgt cggtggttat
     1081 gtcttagaat atcatggttt acgtgccaca caagatgttg atgcttttat ggctctataa
     1141 tatttgtagt gggtaaatcc cctatggata ttatggagcc tatttttgtg tagaaaaaaa
     1201 gtcccatatg acctataatg aaaagcgaca aaacaactca ttagaaagaa tcatatggaa
     1261 caattacatt ttatcacaaa attactagac attaaagacc ctaatatcca gattttagac
     1321 atcatcaata aggatacaca caaggaaatc atcgccaaac tggactacga cgccccatct
     1381 tgccctgagt gcggaaacca attgaagaaa tatgactttc aaaaaccgtc taagatccct
     1441 tacctcgaaa caactggtat gccttctaga attctcctta gaaaacgccg tttcaagtgc
     1501 tatcactgtt caaaaatgat ggtcgctgaa acttctatcg tcaagaagaa tcatcaaatt
     1561 cctcgtatta tcaaccaaaa aattgcgcaa aagttgattg agaagatttc tatgaccgat
     1621 attgctcatc agctggccat ttcaacttca actgtcattc gcaagctcaa tgattctcac
     1681 tttgagcatg atttttcgcg tcttcctgag attatgtcct gggacgttga aacagtccgg
     1741 ggagtgactg tttcaatcgg gagatggaga tgagctttat tgcgcaagat tttgaaaagc
     1801 tcgatatcat cactgttctt gaaggtagaa cacaagctgt catccgagat cactttctta
     1861 aatatgatag agccgtccga tgtcgcgtca aaattattac tatggatatg tttagtcctt
     1921 actatgactt agctagacaa cttttcccgt gtgctaaaat cgttcttgat cgctttca
//
LOCUS       DAVKNL010000118         1690 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVKNL010000118 DAVKNL010000000
VERSION     DAVKNL010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.118"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            <1..263
                     /locus_tag="VW411_002098"
     CDS             <1..263
                     /locus_tag="VW411_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9794737.1"
                     /translation="SQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYWTF
                     LSGKAEKQGITLYHHDQCRSGSVVQEFLGDYSGYVHCDILRQ"
     gene            358..787
                     /locus_tag="VW411_002099"
                     /pseudo
     CDS             358..787
                     /locus_tag="VW411_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295150.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            858..1016
                     /locus_tag="VW411_002100"
     CDS             858..1016
                     /locus_tag="VW411_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HEU9794738.1"
                     /translation="MSLLETAKRHQLNSEKYLSYLLECLPNEETLVNKEVLEAYLPWT
                     KVVQEKCK"
     gene            1109..1690
                     /locus_tag="VW411_002101"
                     /pseudo
     CDS             1109..1690
                     /locus_tag="VW411_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          529 a          305 c          378 g          478 t
ORIGIN      
        1 cgagtcaata ctatttggag cccctttata acctcttgcg agagagacta ttgactcagc
       61 ccttacttca tgcggatgaa acttcttata gggtgctaga gagtgatagt cagctgactt
      121 actattggac ttttttgtca ggtaaagcag agaaacaagg gattacgctt taccaccatg
      181 atcagtgtcg aagtggttca gtagtacaag aattcctagg agattattct ggctatgtgc
      241 attgtgatat tttgcggcag taacttagga ctttagtcct ctagttctgc ctatgcgata
      301 gcagtccaag gtttaggagc aaggcgacgc taagcttggt aaacttcgaa ccgctcgtct
      361 gcttatcgtc aactggaaga agctgaactt gttggatgtt gggcgcatgt gagaaggaag
      421 ttttttgaag cgccccccca agcaagcgga taaatcatcc ttaggagcta aaggtttagc
      481 ttattgtgat cagttatttt ccttggaaag agactgggag gctttgccag ctgatgaacg
      541 actacagaaa cgtcaagaac atctccagcc cttaatggaa gacttctttg cttagtgccg
      601 gcgtcagtca gttttagcag gttcaaaact aggaagggca attgaataca gcctcaagta
      661 tgaagaaacc tttaagacca ttttgaaaga cggacatctg gtcctttcca ataatctagc
      721 tgaacgcgcc attaaatcat tggttatggg acggagtaaa agagtccagt ggactctttt
      781 agcctaagct cagtttaaaa aagcgagggt ggttattttc tcaaagtttt gaaggagcta
      841 aagcaagagc tattattatg agtttgttgg aaacagctaa acgtcatcaa ttaaatagcg
      901 agaaatatct atcctatctt ctagaatgtc ttccaaacga ggaaactctc gtaaacaaag
      961 aggttttaga ggcttattta ccatggacta aagttgtaca agaaaagtgc aaataagaaa
     1021 tctccagatt aggaactatc cgtgagttct ccagtctgga gatttttcaa tagacttcct
     1081 gcgaaacaaa atatggtata atagttctat gaatgatgaa gcaagtaaac aactaaccga
     1141 tgcacgattt aagcgtcttg ttggtgttca acgcacgact tttgaagaga tgttagctgt
     1201 attaaaaaca gcttatcaac ttaaacacgc aaaaggtgga cgaaaaccta aattaagtct
     1261 agaagacctt cttatggcca ctcttcaata tgtgcgagaa tatcgaactt atgaacaaat
     1321 tgcggctgtt tttggtattc acgaaagcaa cttaatccgt cggagccaat gggttgaagt
     1381 aactcttgtt caaagtggtg ttacgatttc aagaactcct ctcagttctg aggacacggt
     1441 aatgattgat gcgacggaag taaaaatcaa tcgccctaaa aaaagaatta gcgaattatt
     1501 ctggtaaaaa gaaatttcac gctatgaagg ctcaagcgat tgtcacaagt caagggagaa
     1561 ttgtttcttt ggatatcact gtgaactatt gtcatgatat gaagttgttc aaaatgagtc
     1621 gcagaaatat cagacaagct ggtaaaatct tggctgacag tggttatcaa gggctcatga
     1681 agatatatcc
//
LOCUS       DAVKNL010000119         1664 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVKNL010000119 DAVKNL010000000
VERSION     DAVKNL010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.119"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(602..1594)
                     /locus_tag="VW411_002102"
     CDS             complement(602..1594)
                     /locus_tag="VW411_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9794739.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYW
                     FYEGGSFIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIVSANGEKFNPYQTITANSL
                     CTFDFDNIKDNFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQPNQI
                     ILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNRKKYKIEIDDDKAL
                     EDVARFLDDHFATNVNQGTLEKISKMSGTKLKNLFKEKYCQSITEYTQRKRMNVAETL
                     LLNTELPIKEIAESVGYSSASKFSIYYKRYKGKLPSEVRNLACKHHNFKCDYCD"
BASE COUNT          579 a          269 c          207 g          609 t
ORIGIN      
        1 attttgatac gattcctgaa aatataatag tagttgacca ttttgaactt acaatcgaaa
       61 aatggttaag taaaagtcaa cagttactat agtttgcaaa ttatttacag aaagaacaaa
      121 aaatgctctg aaacactgat ttcaaagcat tttttgttag tttaaaatta ctcccattct
      181 tcttttccaa acgtacaata tatccaaaac cattcaaaat actagattct attttttata
      241 atatcactaa atccacctaa ttataggacg ttttcagatt tttagtccca gtcccagtac
      301 cggagaaata ttgttttaat ataatatctc tttttgtctt ctaagctctt aaaagcaaaa
      361 gaacaagtaa agagtcaaga caaggataaa aagtccatat tagggcaaat aaaaagcttt
      421 aagacagatg acaaatctaa gtcaaataag aaagaccata gcaaaggtgc agagagataa
      481 atattggcgg tcttcggact gcctttattt ttttatccat ttttcaaatc aaatttattc
      541 agactatata tgcacatata cacttaaatt catataaaaa catggcttgt aaaaaattac
      601 tttaatcaca ataatcgcat ttaaaattgt gatgtttgca agctaaatta cggacttcac
      661 ttggaagttt tcccttgtat cttttataat agatagaaaa tttgctggca gatgaatatc
      721 caacagattc tgctatctct tttataggta gttcagtgtt taaaagaaga gtttcagcta
      781 cattcattct ttttctttga gtgtactctg taatgctttg acaatatttt tccttaaata
      841 aattttttaa tttagtacca ctcattttag atattttttc aagcgtgcct tgatttacat
      901 tcgttgcaaa atgatcatct aagaatcttg ctacatcttc aagtgcttta tcatcatcaa
      961 tttcaatttt atattttttt ctatttaagt atgtgtcaat tactatactt atccattcat
     1021 ttgcctttgc tttaaagaaa aaatcagcgg caggagcgtc catcttacaa tttaatattt
     1081 ccattgccac tctttctaag gcctttgtaa gtattatttg attcggttga agcaaggttg
     1141 aataaaaaga ttctggatta atgttaatag atgctaaatg tttttctatt agctcttttt
     1201 taaaaccaat ggaaacagca agataacaac aattctcgtg taataaaaaa acaaaattat
     1261 cttttatatt atcaaaatca aaagtacata gagagtttgc ggtaatagtt tgatacggat
     1321 taaacttttc tccgtttgca ctgacaatgt aacttgaata aattgaaaca tagtctgaca
     1381 tactataagt gctattttga actacttcct ctttgatata aaaatcatgt atatcgataa
     1441 tgaagctgcc tccttcataa aaccaatata gtccttctgc ataagttgaa ttctcttttt
     1501 tccaacaaaa tgtatgacct gcacttgaat acttctcatt gtttttacat tcatctactt
     1561 tcatataatc ttttacaaaa tcataatatg acataacaca ctatcccttt tagacaatat
     1621 tccaattagc cttattaatt caaaactatt gtattagtaa ttat
//
LOCUS       DAVKNL010000120         1658 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVKNL010000120 DAVKNL010000000
VERSION     DAVKNL010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.120"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(<1..172)
                     /locus_tag="VW411_002103"
     CDS             complement(<1..172)
                     /locus_tag="VW411_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9794740.1"
                     /translation="MVNCEQLEAYRQLEEAALVGCWAHVRRKFFEVPPKQADKSSLGA
                     KGLAYCDQLFSLE"
     gene            complement(245..1021)
                     /locus_tag="VW411_002104"
     CDS             complement(245..1021)
                     /locus_tag="VW411_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9794741.1"
                     /translation="MFAHLDNSVFLKRNKIWKKTLTYPVEREEITYKRKKAKGKRQAL
                     LAQFDSEEVHHQVEESICPDCQGDLKEIGATLQRQELVFIPAQLKRIDHIQHAYKCQA
                     CSDKNPSDKIVKAPIPKAPLAHSLGSASIIAHTIHQKFNLKVPNYRQEEDWAKMGLPI
                     TRKEIANWHIKASQYYLEPLYNLLREKLLEQALLHADETSYRVLESDSQLPYYWTFLS
                     GKAENQAITLYHHDQRRSGLVVQEFLGDYSGYVHCDMLRQ"
     gene            complement(945..1139)
                     /locus_tag="VW411_002105"
     CDS             complement(945..1139)
                     /locus_tag="VW411_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9794742.1"
                     /translation="MKIIQQQSATIDSLTNELALLREQVAYLTQKLYGKSSEKSVCPS
                     GQLSLFEEEQNMEEDSDLPS"
     gene            complement(1216..1556)
                     /gene="tnpB"
                     /locus_tag="VW411_002106"
                     /pseudo
     CDS             complement(1216..1556)
                     /gene="tnpB"
                     /locus_tag="VW411_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          477 a          357 c          311 g          513 t
ORIGIN      
        1 tttccaagga aaataactga tcacaataag ctaaaccttt agctcctaag gatgatttat
       61 ctgcttgctt ggggggcact tcaaaaaact tccttctcac atgcgcccaa catccaacaa
      121 gtgcagcttc ttccagttga cgataagctt ctagctgttc gcagtttacc aagcttagcg
      181 tcgccttact cctaaacctt ggactgctat cgcataggca gaactagagg actaaagtcc
      241 taagttactg ccgcaacatg tcacaatgaa catagccaga ataatctcct aggaattctt
      301 gtactactaa accactccga cgctgatcat ggtggtacag cgtgattgct tgattctcag
      361 ctttcccaga caaaaaagtc caatagtaag gcaactgact atcactctct agaacccgat
      421 aagaggtttc atccgcatga agaagagctt gttctaacaa cttttctcgt aaaagattat
      481 aaaggggctc caaatagtat tgactcgcct tgatatgcca attagcaatt tccttacgtg
      541 tgattggtaa acccatctta gcccaatctt cttcttggcg ataattgggt accttcagat
      601 taaacttctg atggatggtg tgagcgataa tagaagctga gccaaggcta tgcgccaaag
      661 gggctttagg aataggagct ttcacgattt tatcactcgg atttttatca ctgcatgctt
      721 ggcacttata agcgtgttgg atatgatcta ttcgttttaa ttgcgcagga ataaagacta
      781 attcttgtcg ttgaagggtt gctccaatct cttttagatc tccctgacaa tcagggcaaa
      841 tgctctcttc tacttgatga tgaacttctt ctgaatcaaa ttgggcaaga agagcttgac
      901 gtttcccttt agctttctta cgtttatagg tgatttcttc tctttcaact gggtaagtca
      961 gagtcttctt ccatattttg ttcctcttca aaaagactga gttgtccaga tgggcaaaca
     1021 cttttctcag aggattttcc atagagcttt tgcgttagat aagccacttg ttcacgaaga
     1081 agggcaagtt cattggtgag actatcaatt gtagcactct gttgttgaat gattttcaat
     1141 cttcttccat gggcttcctc tccttatttc ctaactttat tataactaaa aagaaagtcc
     1201 tcatttcaat agaaatcacg actttctgat aaatttattt ttggagtgat agaaaagccc
     1261 ttcataagcc aatctacttg ttcaggtgtg agagctttga catccttttc tgtactgggc
     1321 caagtcagtt ttccgttctc aaagcgttta tatagtagcc aaaatccttg accatcccag
     1381 taaagggctt taaagcggtc tttacgtcca ccacaaaaga gaaaaacttg accggagaaa
     1441 ggatctaatt caaagtgggt tttaaccaga taagccaatg aatcaatgcc ttgcctcata
     1501 tccgttttcc cacatacgag atagacctgc cctaggctag atagatgaat tgtcatagag
     1561 caacacttta tccaaaagct gttctaccat ttcgagattg agagattgaa aaaaacttac
     1621 ttcaagcttg ccaatacgaa ttttaagcag aatatcat
//
LOCUS       DAVKNL010000121         1657 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVKNL010000121 DAVKNL010000000
VERSION     DAVKNL010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.121"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            10..1011
                     /locus_tag="VW411_002107"
     CDS             10..1011
                     /locus_tag="VW411_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9794743.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
BASE COUNT          556 a          223 c          333 g          545 t
ORIGIN      
        1 aggactgaaa tgaaaaatat aaatgggaaa aaggtaacta tttatgatat tgcacgttta
       61 tcaggttttt ctccaaaaac agtttcacga gttattaatg gtggagtaaa tgttaaagaa
      121 gaaacttatc aagcgattca aaaagttata gaggaattgt cgtatattcc aaatgcgtat
      181 gcaaaaaatt taactaaaaa agaagctata aatatattga tttctgtaaa aaaaatcgac
      241 tcttttcctt taatttggtt tcatacgctg ttagataaag tgttacggac gtgtaaagaa
      301 tttggtgtta atgctatagt tgagtacttt ggagaggagg atacaattag taattcaatt
      361 atatcaagca caggtagcct agtagatggt gttattgttt tttatgaaag tgtagatgat
      421 atcagaattc agtatttaaa gaagaaccat atgccttttc ttgttttcgg tgaatctcaa
      481 acgtctggag tagtctatgt atctaataat aattttcaag ctacttatga tatgatgaaa
      541 gctgtaactg aagaaaaatt taaaaatatg tggttgctta tgggaggaga atcccatgtt
      601 aataaggatc gtgaaagagg tgtgcgttct tttctgaatg ataaaaatta ctttatggat
      661 ttaaaagtca tttatggctt atctacaatt gattcagttt attcttatgc aatgcagcac
      721 ttgactaccc aaaattatcc ggacattatt tttgtttcag gagatgaaaa ggttcagggc
      781 ttgattcgtg cgtgctatga gaaaggaatt cttattccag atgatatttc tatcattgga
      841 tttgataata ttcctatttc tcagtattat actccagctt tgtctacaat tgctcccaat
      901 tatgttaaat tggctaaaga aatgatagaa ggtgttttag caattattaa aggggaaagt
      961 gtcacatctg ttgaagtttc tcctaaattt gttaggagac agagtttcta gtattgttta
     1021 attcttgaaa atcctagtgg aaaagatgga gtgttctaaa ataagttctg tttagcctcc
     1081 attcagtttc tattgagttg gatccaattt ctaagaagtg ggacatgttc atgttcgatc
     1141 taaggaagac atggtcatat tcgaatgata ttttcaaaaa aatcgtaaaa aagtcttgac
     1201 aaagaaaaaa caaagtatta aaataagttc aacaaatcag aaaagtaact atttttgatc
     1261 ttcagggagc ctgtggtgat tgtgaatagg tggttggaag tagtgaaagt gggctgattt
     1321 tgaaaatgaa tttgaaacaa tgaaaattcg gtgcgcacac cttacagtgc agcttgttgt
     1381 taaacaaggc agagatgtaa agggatagtc cctttataat tgaggtggca ccgcgttacc
     1441 aacgccctca cacggaagta tattctgtgt gtgggctttt ttctatccgt cgtttggttt
     1501 atcttttatt agggccttaa gtcgctttga tgaacttgag ttctatctac agctccatgc
     1561 ctactactaa aagcaaacaa aaaggctgga ctaatacgaa aaaagctagt aatactcaat
     1621 gaaaatcaaa gagcaaacta ggaaactagc cgcaggc
//
LOCUS       DAVKNL010000122         1656 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVKNL010000122 DAVKNL010000000
VERSION     DAVKNL010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.122"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            97..1443
                     /gene="gdhA"
                     /locus_tag="VW411_002108"
     CDS             97..1443
                     /gene="gdhA"
                     /locus_tag="VW411_002108"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEU9794744.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
BASE COUNT          513 a          313 c          334 g          496 t
ORIGIN      
        1 agaattttta gaaaatttct gttttttctt tacagtgaaa aaaaattctg ctataatgtc
       61 ttatgtaata aaatatgaca ataaaaggag aacgatatga catctgctaa agaatatatc
      121 caaagcgtgt ttgaaactgt gaaagctcgt aacgggcacg aggctgaatt cctccaagct
      181 gttgaagaat ttttcaacac tttagaacct gtatttgaaa aacaccctga gtatatcgaa
      241 gaaaatatct tggcacgtat tactgagcct gagcgcgtgg tttctttccg tgttccttgg
      301 gttgaccgtg atggaaaaat tcaagtaaac cgtggttacc gtgttcaatt caactcagct
      361 gttggaccat acaaaggtgg acttcgtttc cacccaactg taaaccaagg gatcttgaaa
      421 ttcctcggat ttgaacaaat ctttaaaaat gtcttgactg gacttcctat cggtggaggt
      481 aaaggtggat cagacttcga tcctaaaggt aaaacagatg ctgaagtgat gcgcttctgc
      541 caaagcttca tgactgaatt gcaaaaacac atcggaccat cacttgacgt acctgctggt
      601 gatatcggtg ttggtggacg tgaaattggt tacctttacg gtcaatacaa acgtcttaac
      661 caatttgatg ctggtgtctt gactggtaaa cctcttggat ttggtggtag cttgattcgt
      721 ccagaagcaa ctggttacgg tttggtttac tatactgaag aaatgctcaa agctaacggt
      781 aacagctttg ctggtaagaa agtcgttatt tcaggttctg gtaacgttgc tcaatatgct
      841 cttcaaaaag caactgaact cggtgcaact gttatttctg tttctgactc aaatggttat
      901 gtcatcgatg aaaatggtat cgacttcgat cttttggttg atgttaaaga aaaacgtcgt
      961 gctcgtttga ctgagtatgc agctgaaaaa gcaactgcaa cctaccacga aggtactgta
     1021 tggacttacg ctggaaacta tgacattgct cttccatgtg ccactcaaaa cgaaatcaac
     1081 ggtgaagcag ctaaacgttt ggttgctcaa ggcgttatct gtgtatctga aggtgccaac
     1141 atgccgagcg accttgatgc catcaaagtt tacaaagaaa atggtatctt ctacggacct
     1201 gcaaaagctg ccaacgctgg tggtgtagcc gtttcagctc ttgaaatgag ccaaaacagt
     1261 cttcgcctct catggactcg tgaagaagtt gatggccgtc tcaaagacat catgacaaac
     1321 atcttcaaca cagctaaaac gacttcagaa acatacggtc ttgataaaga ctaccttgca
     1381 ggagctaaca ttgctgcctt tgaaaatgta gcaaatgcca tgattgcaca aggtattgtt
     1441 taagatttat ttactcaatc ctcaatcact acgattgggg atttttagct tgaattaaaa
     1501 aattggaaaa tctaattaaa taaaatgaaa tgaaataaaa actgaacaaa ttgattgcgg
     1561 aattcaaacc aatttctaac atatttctag aaattaattt gacttttcta atcgatttgt
     1621 tcatatctta tttcaattta ctatacttac ttgacc
//
LOCUS       DAVKNL010000123         1617 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVKNL010000123 DAVKNL010000000
VERSION     DAVKNL010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.123"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            <1..372
                     /locus_tag="VW411_002109"
     CDS             <1..372
                     /locus_tag="VW411_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9794745.1"
                     /translation="FCLVLVSIDYINCLSETTDKNWHKSDRIFVTNTGKTVHSSILSK
                     SLQRANERLKKPIPKHLSPHIFRHTTISILSENKIPLKTITDRVGHPDSEVTTSIYTH
                     VTKNMKDEAINVLDKVMKKIF"
     gene            complement(692..868)
                     /locus_tag="VW411_002110"
     CDS             complement(692..868)
                     /locus_tag="VW411_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794746.1"
                     /translation="MDFLNHSFDTKKVINTKINAVNSKNNVGKNFIDVYREMKEVPNN
                     KIHQSKVNVALIKK"
     gene            complement(871..>1617)
                     /locus_tag="VW411_002111"
     CDS             complement(871..>1617)
                     /locus_tag="VW411_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794747.1"
                     /translation="AITNFSLEISKVEAESNFEFFEAELELIKSIVDDVFKSFPHTDD
                     EVEVIAKSLYKAIFIGYDSTNITGLVIAGYGTDEIFPSIRQIELYGIFSKRLIWKVIN
                     ESVINHHKTCHIIPFAQSEMVETIMNGIDPNLNVYIAEQVSSVMEKNGLGDEIENIFE
                     NISSIQQKYYINPIIDLIGMQPLNEMASTAKTFIELTSFKRKIVNTLETVGGPVDVLA
                     ISKGEGPIWIDRKYYFDIDKNLDYRMRKES"
BASE COUNT          547 a          293 c          212 g          565 t
ORIGIN      
        1 ttttgtcttg ttcttgtttc aattgactat ataaattgtc taagcgaaac aaccgataag
       61 aattggcaca aaagcgaccg tatttttgtt accaatacag gaaaaacagt tcatagttct
      121 atcttgagca agtctctcca gcgagcaaac gaacgcctta aaaaaccaat tcccaaacat
      181 ctgtcccctc acatcttcag acacaccact attagcatct tatcagaaaa taaaattcct
      241 ttaaaaacaa tcacggacag ggttggtcat cccgactctg aagtcactac ttccatctac
      301 acccacgtca caaagaacat gaaagatgaa gcaatcaatg tactggataa agttatgaaa
      361 aagatttttt aaaaagtttt gtcccttttt tgccctctaa atacaaaaat agcccttcgg
      421 ataaaatccg aggggctaga aacgttgtta aatcaacggc cgaacttttg aatttcatgg
      481 ttcgggataa aatagttcac tgaactattt tattttttaa ggttatcata atatcaaata
      541 gttcaattaa atacgctaaa ttactaatat actttttacc tttttcattc taaaatgtaa
      601 agtacaaaca attacaatat actagagggg gagtaaaaaa ggtattaaat cgatgagttc
      661 agcaggcaag aaaatagcac ctttacgggt gctatttttt aattaacgcc acgttaactt
      721 ttgattgatg aattttattg tttggcactt ctttcatttc acggtaaaca tcgatgaaat
      781 tctttccaac attatttttg gagttaactg catttatttt tgtattaata acttttttag
      841 tatcgaaaga atggtttaag aaatccataa ctaactctcc tttctcatcc tgtaatcaag
      901 atttttatca atgtcaaaat agtattttct atcaatccaa attggtcctt ctcctttaga
      961 aatagcaagt acatctaccg gacctcctac tgtttcaaga gtgttgacaa tttttctctt
     1021 aaatgaagtt aattcaataa atgttttagc tgtactcgcc atttcattaa gtggttgcat
     1081 tccaataagg tctattatag gatttatata atatttttgc tgtatagatg atatattttc
     1141 aaatatattc tcaatttcat cacccaatcc atttttctcc ataactgatg atacttgctc
     1201 tgcgatatat acatttaagt taggatctat accattcata atcgtctcaa ccatctctga
     1261 ctgtgcaaaa gggattatat gacaagtttt atgatgattt atcacacttt cattaataac
     1321 tttccaaatt aatcgtttag aaaaaattcc atataattca atttgtctta tagatggaaa
     1381 tatctcgtct gtaccataac ctgctataac taatccagtt atgtttgttg agtcatatcc
     1441 aatgaaaatc gctttatata aagatttagc aataacttca acctcatcat cagtatgagg
     1501 aaaggattta aaaacatcgt ctacaatgct ttttattaac tctaactcag cttcaaaaaa
     1561 ttcaaaatta ctttcagctt ctacttttga aatttctaaa ctaaaattag ttatagc
//
LOCUS       DAVKNL010000124         1385 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVKNL010000124 DAVKNL010000000
VERSION     DAVKNL010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.124"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            <1..1139
                     /locus_tag="VW411_002112"
     CDS             <1..1139
                     /locus_tag="VW411_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEU9794748.1"
                     /translation="EKVNVQNITSEVISKNSDIKLTGEEEFWINKWDNFNTKQLIIGR
                     KLDIHLSIVSSITNVLQIILPVLTLIVGVNIKTFEQLTLGQIVAISTVSPYFISPIIS
                     LSDNYIQLMLLKGYFLRIEDVFNTKSELIPERVSQDIKFDKKIELKDIWYKYGLFDDY
                     VLKGINVTIKKGETVAIVGESGSGKSTLAKILLGLLEPNIGSIEVDGVEKEEIGQTLY
                     RKIFGAVLQNSTLSYGTLRENLTFGHFVSDEELMTNLNSIGLSNVVKSLPLGLETIIA
                     EEGNNFSGGQQQMILLARCLLSKPSVVVLDEATSSLDNLSQQITTSYLSEIGTTKILI
                     AHRLDTIKSADKILVMHNGEIVEIGTHRELLELGGIYKQLYSNN"
     gene            1163..>1385
                     /locus_tag="VW411_002113"
     CDS             1163..>1385
                     /locus_tag="VW411_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU9794749.1"
                     /translation="MKIMKKKYWTLAILFFCLFNNSVTAQEIPKNLDGNITHTQTSES
                     FSESDEKQVDYSNKNQEEVDQNKFRIQIDK"
BASE COUNT          522 a          165 c          238 g          460 t
ORIGIN      
        1 aagaaaaggt taatgttcaa aatattactt ccgaagtaat ttctaaaaat agtgatatta
       61 agctaactgg agaagaggaa ttttggatta acaaatggga taattttaat acaaaacagc
      121 tcatcatagg tcgaaaactt gatatacatt tatcaattgt tagtagtata acgaatgttt
      181 tacaaattat tctccctgtt ttgaccctta ttgtaggtgt aaatataaaa acattcgaac
      241 aattgacgtt aggacaaatt gtagcaataa gtacagtctc accatacttt atttctccta
      301 taatttcttt aagtgataac tatatacaat taatgttatt aaagggatat tttttaagaa
      361 tagaggatgt gtttaatact aaatccgaat taattccaga aagagtcagt caagatataa
      421 aatttgataa aaaaatagaa ttaaaagata tttggtataa atatggatta tttgatgatt
      481 atgttttgaa aggaataaat gttactatta aaaaaggaga aactgttgct attgttggag
      541 aatcaggttc aggtaagagt acattagcta aaattttatt aggtttatta gaacctaata
      601 ttggttcaat agaagttgat ggagtagaaa aagaagaaat tggtcaaaca ttgtatagaa
      661 agatttttgg agcagtgtta caaaattcaa ccctaagtta tggtacctta agagagaatt
      721 tgacatttgg acactttgtt tcagatgaag aattaatgac aaatctaaat tcaattggtc
      781 ttagcaatgt agttaaatct ttacctcttg gattagagac aatcatcgct gaagaaggta
      841 ataacttttc tggagggcag cagcaaatga tacttttagc tcgttgtctt ttgtcgaaac
      901 cttcggtagt tgttttggac gaagcaacaa gtagtttaga taatttatct caacaaatta
      961 caacttctta cttaagtgaa atcggtacca ctaagatttt aattgcccat cgactagata
     1021 ctatcaagtc tgcagataag atcttagtaa tgcataatgg tgaaattgta gagattggga
     1081 cccatagaga acttcttgaa ctaggaggca tttataagca attgtattca aataattagt
     1141 ttttgattaa aagggtaaat ttatgaagat tatgaaaaaa aaatattgga ctttagcgat
     1201 attattcttt tgtttgttca ataattctgt tactgctcaa gaaataccta aaaatcttga
     1261 tggcaatata actcacactc agactagcga aagtttttct gaatctgatg aaaaacaggt
     1321 tgactattct aataaaaatc aagaagaagt agaccaaaat aaatttcgta ttcaaatcga
     1381 taaga
//
LOCUS       DAVKNL010000125         1348 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVKNL010000125 DAVKNL010000000
VERSION     DAVKNL010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.125"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            <1..317
                     /locus_tag="VW411_002114"
     CDS             <1..317
                     /locus_tag="VW411_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU9794750.1"
                     /translation="IEEKKLPLFGTPGFYETNLNLLNNQPEDIMGLVLLLYWSQNLDD
                     FNVFSKMSFLEAMEYAMNFNSRRLDTLDKSDWLYDIYKKAFNYKYDTFSELKKDFIEV
                     LK"
     gene            331..1248
                     /locus_tag="VW411_002115"
     CDS             331..1248
                     /locus_tag="VW411_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9794751.1"
                     /translation="MNCFLKMNNVSVRYDDVIALKDITLQINKGDFIGLLGSNGAGKS
                     TLINSIVGFQEIYLGEIEYCDKDLIVSSQPFAHLGFTPQTTVIDFYTTVKDNVILGLN
                     LAGKFGKNAEKLCQIALEIVGLADKKNNLVETLSGGQLQRVHIARAIAHNPDFYILDE
                     PTVGLDTESAEKFLMYLKDKSLEGKTIIISSHDINLLEKFCKKILFLQNGSISFFGDM
                     RDFVDNSTIKLNFSMQNRISRYQIEFLENFRFKVHIEDNDSFTIEVPIEEKILDVINE
                     VGKACEIKNFSTSKLTLQESYLQRIGGEK"
     gene            1245..>1348
                     /locus_tag="VW411_002116"
     CDS             1245..>1348
                     /locus_tag="VW411_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9794752.1"
                     /translation="MKADQLRHKSDLGLRGLAIIAKNEIIAFFRSKGL"
BASE COUNT          492 a          145 c          247 g          464 t
ORIGIN      
        1 aaattgaaga aaaaaagttg cctctatttg gaactccagg gttttatgaa accaacttaa
       61 atttattgaa taatcaacca gaggatatta tgggattggt tttgttatta tattggagtc
      121 agaatttaga tgattttaac gtcttttcaa aaatgagttt tttagaagcg atggagtatg
      181 caatgaattt taattcgcgt cgcttagaca cacttgataa atcagattgg ctttatgata
      241 tttataaaaa agcgttcaat tataaatatg atactttttc tgaattaaaa aaagatttta
      301 tagaggtatt aaaataaagg taggggtgtt atgaattgtt tcttgaaaat gaataatgta
      361 agtgttcgtt atgatgacgt aatagcttta aaagatataa ctttacaaat aaataaggga
      421 gatttcattg gcttattagg ttcaaatggt gcaggtaaat ctacgttaat taattctatt
      481 gtaggttttc aagagattta tttaggagaa atagagtatt gtgataaaga tttgatagtt
      541 agttctcaac cttttgctca tttaggcttt actcctcaaa ccacagtaat tgatttttat
      601 actactgtga aggacaatgt aatattgggg ctgaaccttg ctggaaagtt tgggaaaaat
      661 gctgagaagt tgtgtcaaat agccttagaa attgttgggt tagctgataa aaaaaataat
      721 ttggtagaaa cattgtcagg tggacaactg caacgcgtcc atattgctag agcaatagct
      781 cataatccag atttttatat tttagatgaa cctaccgttg gtttagatac tgaatctgcc
      841 gaaaaatttt taatgtattt aaaagataag agtttggaag gaaaaactat tatcatatct
      901 tcacatgaca taaatctact cgaaaagttt tgtaaaaaaa tacttttttt acaaaatggc
      961 tccatatcat tttttggtga tatgcgtgac tttgtagata attcaactat caaattaaat
     1021 ttttcaatgc agaatagaat ttctagatat caaattgaat ttttagaaaa ttttagattt
     1081 aaagttcaca tcgaagataa tgatagtttt acaatagaag tccctataga agaaaagatc
     1141 ttagatgtta tcaatgaggt aggaaaagca tgtgaaatta aaaacttttc aacaagtaaa
     1201 ttaaccttac aagaaagtta tttgcaaaga ataggaggag aaaaatgaag gctgatcaat
     1261 taaggcacaa atcggactta ggtttaagag gtctagcgat tattgctaaa aatgagatta
     1321 ttgctttttt tagaagtaaa ggtttaat
//
LOCUS       DAVKNL010000126         1304 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVKNL010000126 DAVKNL010000000
VERSION     DAVKNL010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.126"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            145..>1304
                     /locus_tag="VW411_002117"
     CDS             145..>1304
                     /locus_tag="VW411_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSpn5 family transposase"
                     /protein_id="HEU9794753.1"
                     /translation="MNSLPNHHFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKE
                     RISKYLVTNDQRRYCRYSDSDILVQFLFQLLTGYGTDYACKELSADAYFPKLLEGGQL
                     ASQPTLSRFLSRTDEETVHSLRCLNLELVEFFLQFHQLNQLIVDIDSTHFTTYGKQEG
                     VAYNAHYRAHGYHPLYAFEGKTGYCFNAQLRPGNRYCSEEADSFITPVLERFNQLLFR
                     MDSGFATPKLYDLIEKTGQYYLIKLKKNTVLSRLGDLSLPCPQDEDLTILPHSAYSET
                     LYQAGSWSHKRRVCQFSERKEGNLFYDVISLVTNMTSGTSQDQFQLYRGRGQAENFIK
                     EMKEGFFGDKTDSSTLIKNEVRMMMSCIAYNLYLFLKHLAGGDFQTLTIKRFR"
BASE COUNT          376 a          292 c          243 g          393 t
ORIGIN      
        1 ctaaaaacca tccagaatcc ttgccttagc ttagatcctg gatggtttct tttttcaccc
       61 aatgggtgtt ttttactaga caaaaaagag tttccccttt atggtataag tgtagaaaaa
      121 aacacaaaaa gaaaggaaac tcacatgaac agtttaccaa atcatcactt ccaaaacaag
      181 tctttttacc aactatcttt cgatggaggt catttaaccc agtatggtgg tcttatcttt
      241 tttcaggaac ttttttccca gttgaaacta aaagagcgga tttctaagta tttagtaacg
      301 aatgaccaac gccgctactg tcgttattcg gattcagata tccttgtcca gttcctcttt
      361 caactgttaa caggttatgg aacggactat gcttgtaaag aattgtcagc tgatgcctac
      421 tttccaaaat tgttggaagg agggcagctt gcttcacagc caaccttatc ccgttttctt
      481 tccagaactg acgaggaaac agtccatagt ttgcgatgcc tcaaccttga attggtcgaa
      541 ttctttttac agtttcacca gctaaaccaa ctcattgtag atatcgattc tacccatttc
      601 acaacttatg gcaagcaaga aggtgttgct tataacgccc actatcgtgc tcatggctat
      661 catcctcttt atgctttcga ggggaagaca ggttattgtt tcaatgccca gcttcgtcct
      721 ggtaatcgtt attgttctga agaggcagac agctttatca cacctgtttt agaacggttt
      781 aatcaacttc tctttcgaat ggatagtggc tttgcgaccc caaaattata cgatttaatt
      841 gaaaaaacag ggcaatacta cctcataaaa ctcaagaaaa atactgttct gagccgtctt
      901 ggagaccttt ccctcccttg cccacaggat gaggacttaa ccatcttgcc ccactccgcc
      961 tactcagaaa ctctctatca agcaggatct tggtcgcaca agcgtcgtgt ctgccagttc
     1021 tctgaacgaa aagaaggaaa cttgttctac gatgttattt ctctcgttac aaatatgacg
     1081 agtggaacaa gccaagacca gtttcagctt tatcgtggac gtggtcaagc cgagaatttc
     1141 atcaaggaga tgaaggaggg attttttggc gataaaacgg atagttcaac cttaatcaaa
     1201 aacgaagttc gtatgatgat gagctgtatc gcctacaatc tctatctttt tctcaaacat
     1261 ctagctggag gtgacttcca aactttaaca atcaaacgct tccg
//
LOCUS       DAVKNL010000127         1058 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVKNL010000127 DAVKNL010000000
VERSION     DAVKNL010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.127"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            <1..559
                     /locus_tag="VW411_002118"
     CDS             <1..559
                     /locus_tag="VW411_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9794754.1"
                     /translation="NKDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGT
                     EIPWVSISDMPISGYVTNARESISKLALKSKKIDISPKGTLLMSFKLSIGKVAILDIP
                     ATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPI
                     SNHEEMKRIIFKVDLLFQKVSQLFE"
     gene            complement(513..>1058)
                     /locus_tag="VW411_002119"
     CDS             complement(513..>1058)
                     /locus_tag="VW411_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9794755.1"
                     /translation="IPMNWVVIKIKDIFSINTGLSYKKGDLSINNKGVRIIRGGNIKP
                     LEFSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHIGKFARIDKDYDGVVAGGF
                     IFQLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQALYNIPKTTLSELLIPLAP
                     FEEQELITQKVEKLFEKVNQL"
BASE COUNT          380 a          167 c          155 g          356 t
ORIGIN      
        1 gaacaaagac gaaacaacct cttatcctat atatgaaatc ccagaagcat ggagatatat
       61 taaatttgct agcctagtta acttcagaat aggaaaaact cctccacgca gtgaagctac
      121 tttttgggga actgaaatac cttgggtatc tatatcagat atgcctatat ctggttatgt
      181 aactaatgcg agagaatcta tttctaaatt agcattaaaa tcaaagaaaa tagacatttc
      241 acctaaagga actttattaa tgagttttaa attatctatt ggaaaagttg ccatattgga
      301 tattcctgcc actcataatg aagccattat atcgatattc ccctatgcta ataaagaaaa
      361 tattatcaga gattacttaa tgatattttt gccacttatc tctactttag gtgattcaaa
      421 agatgctatc aaagggaaaa cattaaatag taccagtatc tccgaattat taatccctat
      481 ttctaatcat gaagagatga aaagaatcat tttcaaagtt gatttacttt ttcaaaaagt
      541 ttctcaactt tttgagtaat aagttcctgt tcctcaaaag gagctaacgg aattaatagc
      601 tcgctcagtg tagttttagg aatattatat aaagcttgac ctgatagttt agttattgct
      661 ttcaattgtt tataaaataa cggagaggac aagttaaata atagaaattt tgaaataatc
      721 tctgaacttt cgaatggtgt taattggaaa ataaatccac cagccacaac accatcatag
      781 tctttatcga ttcttgcaaa ctttccaata tgttctaaag aggttgatac aggtgttatt
      841 agctgattat gttttaaata aacttgctca gaggagatga attgtgtatc aatgtagtaa
      901 tcattatcca acagagaaaa ttctaaaggc ttaatattac caccacgtat aattctaaca
      961 cctttattat taatgcttaa atcgcccttc ttgtaagaaa gacctgtatt tattgaaaaa
     1021 atatctttta tttttataac aacccaattc ataggtat
//
LOCUS       DAVKNL010000128         1044 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVKNL010000128 DAVKNL010000000
VERSION     DAVKNL010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.128"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            1..1044
                     /locus_tag="VW411_002120"
                     /pseudo
     CDS             1..1044
                     /locus_tag="VW411_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          396 a          170 c          199 g          279 t
ORIGIN      
        1 tgataaagtt cagatctatg aacttagaaa acaaggatat agcttagaga agctttcaaa
       61 taaatttggg ataaacaatt ctaatcttag gtacatgatt aaattgattg atcgttacgg
      121 aatagagttc gtcaaaaaag gaaaaaatcg ttactattct cctgatttaa aacaagaaat
      181 gattcataaa gtctgacatg aaggctggac taaagataga gtttctcttg aatactgtct
      241 cccaagtcgt acgatacttc ttaactggct agcacaatac aggaaaaacg ggtatactat
      301 tgttgagaaa acaagaggga gagtacctga gagcggagaa tgccatccta aaaaagttaa
      361 gagaactccg attgaaggag gaaaaagaga aagaagaaag acagaaatta ttcaagaatt
      421 aatgactgag ttttcgttag atattcttct aaaagccatt aaactagctc gtttgaccta
      481 ctactatcac ttgaaacagc tagataaacc agataaggac caagagctta aagctgaaat
      541 tcaatccatt tttatcgaac acaagggaaa ttatgcttat cgtcggattt atttagaact
      601 aagaaatcgt ggttatctgg taaatcataa aagagttcaa ggcttgataa aagtactcaa
      661 tttacaagct aaaatgcgac agaaacgaaa atattcttct cataaaggag acgttggcaa
      721 gaaggcagag aatctcattc aaggacaatt tgaaggctct aaaacaatgg aaaagtgcta
      781 cacagatgtg acagaatttg ccattccagc aagtactcaa aagctttact tatcaccagt
      841 tttagatggc tttaacagtg aaattattgc ttttaatctt tcttgttcgc ctaatttaga
      901 acaagtacaa acaatgttgg aacaggcatt caaagagaag cactacgaga atacgattct
      961 ccatagtgac caaggctggc aatatcaaca cgattcttat catcggttcc tagagagtaa
     1021 gggaattcaa gcatccatgt cacg
//
LOCUS       DAVKNL010000129         1026 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVKNL010000129 DAVKNL010000000
VERSION     DAVKNL010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.129"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            <1..574
                     /locus_tag="VW411_002121"
     CDS             <1..574
                     /locus_tag="VW411_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9794756.1"
                     /translation="KIPSRLYKIDTGKVLFDGVNINQIDKKILSQNLGVVPQDSFLLN
                     RSILDNITLKHEVTSQKIEEVCKAVQIYDEIMAMPMKFNTIISEMGSNISGGQRQRIA
                     LARALINNPSIVILDEATSALDTINEERITKYIQSQGCTQIIVAHRLSTIKDADVIFV
                     MKGGKIVESGNHKYLMDLGGEYYSLYTKRK"
     gene            587..757
                     /locus_tag="VW411_002122"
     CDS             587..757
                     /locus_tag="VW411_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794757.1"
                     /translation="MKKENEYVILTTASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
BASE COUNT          374 a          127 c          197 g          328 t
ORIGIN      
        1 aaaaatacca tcaagattat ataagattga tacaggaaaa gttttatttg atggtgtaaa
       61 tattaatcaa atagataaaa aaatattaag tcaaaattta ggagtagttc cacaggattc
      121 atttttattg aaccgaagta ttcttgataa tataacttta aagcacgaag ttacttcaca
      181 aaagatagag gaagtttgta aagcagttca aatctatgat gaaatcatgg ctatgccgat
      241 gaaatttaat actatcatct cagagatggg gtcaaatatt tcaggtgggc aaaggcaacg
      301 gatagcactg gcacgtgcat taataaataa tcctagtatt gtaattttag atgaagcaac
      361 tagtgcatta gacactatta atgaggaaag aataacaaag tatatacaaa gtcagggctg
      421 tactcaaata attgtagctc atagattgtc aacgattaag gatgcggatg ttatttttgt
      481 aatgaaaggt ggtaagattg ttgagtcagg aaatcataag tacttaatgg atcttggtgg
      541 agagtactac agcttatata caaaaaggaa atgaggtgta aagaaaatga agaaagaaaa
      601 tgaatatgta attttaacaa cagcctcact aggggtgatg attggaatag tgtttgcaat
      661 ttttttagat tttccagttg aatatggtat ttctttaggc ttgttgaatg gaatagtatt
      721 gggttcgctg attgtttaca aaaacaataa gaattaagca taattttttg ctgtaaacta
      781 aggagtagag atggctatag ttgaaattat aaatctaaca aaaagcttta aagatattga
      841 agttattcat aacacttaaa taatagagca actacagtag tagcttaaaa acatgattaa
      901 atcgctattc ttaggagtag cggtttttct ttttgtttaa tactctttga aaatctcttc
      961 aaaccacgtc agctttgctt taccgtactc aagtacagcc tgcggctcgc ttcctagttt
     1021 gctctt
//
LOCUS       DAVKNL010000130         1004 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVKNL010000130 DAVKNL010000000
VERSION     DAVKNL010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.130"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            180..977
                     /locus_tag="VW411_002123"
                     /pseudo
     CDS             180..977
                     /locus_tag="VW411_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          345 a          207 c          152 g          300 t
ORIGIN      
        1 cagcctcaac aagataaaaa taggaaaagt tgacaaaata gaaacaaatt tgctacctaa
       61 atttcaaaca attaaagttc taaaaagacc gccttaaaac ttttgggtaa atcctattag
      121 aactataata tttttatata ggtattatgg ttctgatttt ctagtataaa aaaattcaaa
      181 ctagaattct tcttagaaaa ggccgtttta agtgctatca ttgctcaaaa atggagctcg
      241 ctgagacttc tatcgtcaag aagaatcatc aaattccttg tatcatcaac caaaagattg
      301 ctcagaagct aattaaaaag acttctatga ccgacattga tcatcagttg tctatttcaa
      361 cttcaactgt cattcgcaag aacaatgatt ttcactttga gcatgatttt tcgcgtcttc
      421 ctgagattat gtcctaggac gttgaaacag tccggggagt gactgtttca atcgggagat
      481 ggagatgagc tttattgctc aggactttga taaactcaat atcataactg ttcttgagag
      541 cagaacacaa gccatcatcc gaaatcccat gaatacaagg ctatcaagcg ctactggaag
      601 ctcattcaac aagatagtca gaaactgagt gataaacgat tttatcgccc tacttttcgt
      661 atgcacttga ctaataaaga aattctagat aaacttttga gctattccga agactttaaa
      721 caccactata atctctatca gctcttgctt ttccacctct gtttcagact ttgtttaaaa
      781 ccttcctcaa agataaagag aaaatcgtca acgcccttca attaccttat tccaacgcca
      841 aactggaagc caccaataat ctcatcaaac ttatcaagca caatgccttt ggttttagga
      901 actttgaaaa cttcaaaaaa gaaaggacga aatttgtcct ttctagatct tcgctttctt
      961 caacccacta cagttgacaa agaaccgaaa aatgtctcgc ctct
//
LOCUS       DAVKNL010000131          939 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVKNL010000131 DAVKNL010000000
VERSION     DAVKNL010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..939
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.131"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            76..258
                     /locus_tag="VW411_002124"
     CDS             76..258
                     /locus_tag="VW411_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HEU9794758.1"
                     /translation="MANDNKSHYLIYRVLGISFEEGENIDLYQNKGRFLYKYAGSFLE
                     EAAVLCFNEKFGTENT"
     gene            268..633
                     /locus_tag="VW411_002125"
     CDS             268..633
                     /locus_tag="VW411_002125"
                     /inference="COORDINATES: protein motif:HMM:NF021048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HEU9794759.1"
                     /translation="MPNSESTKPKTFEIDCLVGEKHAYEIKWWDATTDGDHITKEHTR
                     IKVIHNKGYIPIRLMFYYPNRTQAIKIQQTLETLYNGIGGKYYYGDSAWEHLRAVTGI
                     DLLSILTDIANKKTGVKSK"
     gene            630..>939
                     /locus_tag="VW411_002126"
     CDS             630..>939
                     /locus_tag="VW411_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEU9794760.1"
                     /translation="MTVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIM
                     YSFEDTWTSIEDYKEFLSVRLEECKRVLKNSGSIFVHCDKIANHHIRLILDNIFGVDM
                     F"
BASE COUNT          384 a          115 c          156 g          284 t
ORIGIN      
        1 aaagaaaaaa tcgaaaaatt agctaagaaa tattctgata acttaaacat caaagttcaa
       61 gagagagttc gtgaaatggc aaatgataat aagagccatt atttgatata cagagtttta
      121 ggtatttcat ttgaagaagg agaaaatatc gatttgtatc aaaataaagg tcgtttttta
      181 tacaaatatg ctggttcatt tttagaagaa gctgcagtac tatgctttaa cgaaaaattt
      241 ggtacagaaa atacttaaaa agttaacatt cctaattctg aaagtacaaa acctaagact
      301 tttgaaattg attgtttagt cggagaaaaa cacgcatacg aaataaaatg gtgggatgca
      361 actacagatg gagaccatat aactaaagaa cacactagaa taaaagttat tcataacaaa
      421 ggatatatac caattcggtt aatgttctac tatccaaata gaactcaagc tataaaaatt
      481 cagcaaactt tagaaacatt gtataacggt attggaggga aatattatta tggagattct
      541 gcctgggaac atttaagagc agtgaccggt attgatttac ttagtattct aacagatatt
      601 gcaaataaaa aaacaggggt aaaatcaaaa tgacagtatt aaaaggagat aacttagaaa
      661 tattaaaaac tattgaatcc tcaagtattg atttaatcta tatggaccct cctttcttta
      721 cacagaaaac ccaaaaatta tctaataaca aaaatattat gtattcattc gaagatacgt
      781 ggacttcgat tgaggattac aaagaatttt tgtctgtaag attagaagaa tgcaaaagag
      841 tgctaaaaaa tagtggcagt attttcgttc attgtgataa aattgcaaat catcatatta
      901 gattaatttt agataatatc tttggagtag atatgtttc
//
LOCUS       DAVKNL010000132          915 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVKNL010000132 DAVKNL010000000
VERSION     DAVKNL010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.132"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            <1..>915
                     /locus_tag="VW411_002127"
     CDS             <1..>915
                     /locus_tag="VW411_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU9794761.1"
                     /translation="ETTGMPTRILLRKRRFKCYHCSKMMVAETSIVKKNHQIPRIINQ
                     KIAQKLIEKISMTDIAHQLSISTSTVIRKLNDFHFKHDFSCLPEIMSWDEYAFTKGKM
                     SFIAQDFNNLNIITVLEGRTQAIIRNHFLKYDRAVRCRVKIITMDMFSPYYDLARQLF
                     PCAKIVLDRFHIVQHLSRAMSRVRVQIMNQLDRKSHEYKAIKRYWKLIQQDSRKLSDK
                     HFYRPTFRMHLTNKEILNKLLSYSQDLKHHYQLYQLLLFHFQNKEPEKFFGLIEDNLK
                     QVHPLFQTVFKTFLKDKEKIVNALQLHYS"
BASE COUNT          287 a          192 c          149 g          287 t
ORIGIN      
        1 tgaaacgact ggtatgccta ctagaattct ccttagaaag cgtcgattca agtgctatca
       61 ctgttcaaaa atgatggtcg ctgaaacttc tatcgtcaag aagaatcacc aaatccctcg
      121 tatcatcaac caaaaaattg ctcaaaagtt aattgaaaag atttctatga ctgatattgc
      181 ccatcagctt tccatctcaa cttcaactgt tattcgtaag ctcaatgact ttcactttaa
      241 acatgatttt tcttgtcttc ctgagattat gtcttgggat gagtatgctt ttacaaaggg
      301 aaagatgagc ttcattgcgc aagattttaa caatctcaat atcatcactg ttcttgaggg
      361 aagaacacaa gctatcattc gaaatcactt tcttaaatat gatagagccg tccgatgtcg
      421 cgtcaaaatt attactatgg atatgtttag tccttactat gacttagcta gacaactttt
      481 cccatgtgct aaaatcgtgt tggatcgctt tcacattgta caacatctta gccgtgctat
      541 gagtcgtgtg cgtgttcaaa tcatgaatca attggatcga aagtctcatg aatacaaggc
      601 tatcaagcgc tactggaagc tcatacaaca ggatagtcgt aaactcagcg ataaacattt
      661 ttatcgccct acttttcgta tgcatttaac caataaagag attttaaaca agcttttgag
      721 ctattcacaa gacttgaaac atcactatca gctctatcaa ctcttgctgt ttcactttca
      781 gaataaggaa ccggagaaat ttttcggact cattgaggac aatctaaagc aggttcatcc
      841 tctttttcag actgtcttta aaacctttct aaaggacaaa gagaaaatcg tcaacgccct
      901 tcaactacac tattc
//
LOCUS       DAVKNL010000133          884 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVKNL010000133 DAVKNL010000000
VERSION     DAVKNL010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.133"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            81..560
                     /locus_tag="VW411_002128"
     CDS             81..560
                     /locus_tag="VW411_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU9794762.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWEQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
     gene            654..725
                     /locus_tag="VW411_002129"
     tRNA            654..725
                     /locus_tag="VW411_002129"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:687..689,aa:Glu,seq:ttc)
BASE COUNT          337 a          121 c          206 g          220 t
ORIGIN      
        1 gagctaaagg gaacagaatg gagaaaatgg aacaaatgtg ttttctaatc tgttagactg
       61 tatctagaaa ggggaaaatt atgattaaag aattgtatga agaagtccaa gggactgtgt
      121 ataagtgtag aaatgaatat taccttcatt tatgggaatt gtcggattgg gagcaagaag
      181 gcatgctctg cttacatgaa ttgattagta gagaagaagg actggtagac gatattccac
      241 gtttaaggaa atatttcaag accaagtttc gaaatcgaat tttagactat atccgtaaac
      301 aggaaagtca gaagcgtaga tacgataaag aaccctatga agaagtgggt gagatcagtc
      361 atcgtataag tgaggggggt ctctggctag atgattatta tctctttcat gaaacactaa
      421 gagattatag aaacaaacaa agtaaagaga aacaagaaga actagaacgc gtcttaagca
      481 atgaacgatt tcgagggcgt caaagagtat taagagactt acgcattgtg tttaaggagt
      541 ttactatccg tacccactag taagtcatgc aaaaaaaatg aaaaaaatta gaaaaagtag
      601 ttgacaaagt ttgaaaaggc tgtataatag taagagttga aaataacaac tcaggtccgt
      661 tggtcaaggg gttaagacac cgccttttca cggcggtaac acgggttcga atcccgtacg
      721 gactatggta tgttgcgtca ggaccacttg atgaaaaaaa gtttaaaaaa acttaaaaat
      781 cttcaaaaaa gtgttgacaa gcgaaagcag ttgtgatata ctaatatagt tgtcgcttga
      841 gagaagcaag tgacaaagac ctttgaaaac tgaacaagac gaac
//
LOCUS       DAVKNL010000134          778 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVKNL010000134 DAVKNL010000000
VERSION     DAVKNL010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.134"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            217..468
                     /locus_tag="VW411_002130"
     CDS             217..468
                     /locus_tag="VW411_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000098314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794763.1"
                     /translation="MSSQMKAFLNELQGNMEVVGEFFDNPEKVIRKFGIVGREKEALL
                     IRDLNDMDDYALSIQNSVASPSGAHSSTCHFVRQDLQSA"
BASE COUNT          289 a           81 c          136 g          272 t
ORIGIN      
        1 tagttcctat aagtacagaa acctatttta atttactaat ttcaaaacaa ttttttgaga
       61 tatatgtaat taaatctaaa ttaagcttta aaaaattatt tttagcaatt tgattttgaa
      121 agtgtcatgc agcaatatga aagcgtagaa tagaggtgtt tccaatgtac aagtaagttt
      181 tgattccaaa taaagtaata gaaaaggagc tatataatga gtagtcaaat gaaagctttc
      241 ttgaatgaac ttcaaggaaa tatggaagtt gttggagaat tttttgataa tcctgaaaag
      301 gtaattagaa aatttggaat tgtcggaaga gagaaggaag cattgctaat ccgtgactta
      361 aatgatatgg atgattatgc tctttcaatt caaaatagcg ttgcatcgcc ttctggggca
      421 catagtagca cttgccattt tgttagacaa gatttacaat ccgcataatt gttaatatat
      481 aaaacataaa atttcaattt tcaattaggg ataatagaat tccttactat gatatagaaa
      541 gtgaattaat taggaaatct tagtcagagg tttctgatta attcattttt tatatattaa
      601 gagtgatgtg aaatgttcaa attgtgagac tactctcgga ttgttgtata gaaatttaaa
      661 attttattgt gagcttttta aggaagagtg gccgatatga aaaataaata cgatgttaaa
      721 agaattattc cggatgaatt atcagaatca ctagatattt ttctaaaaaa ttattctg
//
LOCUS       DAVKNL010000135          776 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVKNL010000135 DAVKNL010000000
VERSION     DAVKNL010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.135"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(1..776)
                     /locus_tag="VW411_002131"
                     /pseudo
     CDS             complement(1..776)
                     /locus_tag="VW411_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          242 a          135 c          167 g          232 t
ORIGIN      
        1 gtttggcgtt agaatagtgt agttgaaggg cgttgataat cttttcttta tctttgagga
       61 aggttttaaa gacagtctga aaaataggat gaacttgctt aagattgtcc tcaataagtc
      121 cgaaaaattt ctccggttcc ttattctgaa agtgaaacag caagagttga tagagctgat
      181 agtgatgttt caagtcttgt gaatagctca aaagcttgtc taaaatctct ttattggtta
      241 aatgcatacg aaaagtagga cgataaaatc gcttatcact cagtttacgg ctatcctgtt
      301 gtatgagctt ccagtagcgc ttgatagcct tgtattcatg ggattttcga tccaattggt
      361 tcataatttg aacacgcaca cgactcatag cacggctaag atgttgtaca atgtgaaagc
      421 gatccaacac gattttagca ttcgggagtg aaacagtctg ggagactgtt tcagcctgag
      481 cctagaaatt tgaaagcgaa gctgtttagc caagtcatag taaggactaa acatatccat
      541 cgtaatgatt ttcacttgac aacgaacggc tctatcgtag cgaagaaagt gatttcggat
      601 gacagcttgt gttctgcctt caagaacagt gataatatta agattatcaa aatcttgcgc
      661 aatgaaactc atctttccct tagtgaaggc atactcatcc caagacataa tctttggaag
      721 ccgagaaaaa tcatgctcaa agtgaaagtc attgagcttg cgaatgacag ttgaag
//
LOCUS       DAVKNL010000136          690 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVKNL010000136 DAVKNL010000000
VERSION     DAVKNL010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.136"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            100..462
                     /locus_tag="VW411_002132"
     CDS             100..462
                     /locus_tag="VW411_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9794764.1"
                     /translation="MLEVGLNFLISLFTFTFDILYPIVKVGHTDDYSHGAVKLSVSLV
                     DKDAMKKIFVTVIGYFEINIDENITDILYVNGTAILYLYLRSIVSIVSAIDSSEAMLL
                     PIINVLELLDKSQPFEEE"
BASE COUNT          226 a           89 c          128 g          247 t
ORIGIN      
        1 ctgactacgt cagttccatc tacaacctca aaacagtgtt ttgagcaacc tgcggctagt
       61 ttcctagttt gctctttggt tttcattgag tataacacat tgttagaagt tggtttaaat
      121 ttcctaatca gtttgttcac atttaccttc gatatattat atcccatagt taaggttggt
      181 catacagatg attatagtca tggagccgta aaacttagtg tttctttagt tgacaaagat
      241 gccatgaaaa aaatatttgt aactgtaata ggatattttg aaataaatat agatgaaaat
      301 atcaccgata ttctatacgt aaatggtact gctattcttt atctttattt acgttcaatt
      361 gtttcaatag tttcggcaat tgatagcagt gaagcaatgt tgctacctat cattaatgtt
      421 ttagagttac tagataaatc tcaacctttt gaagaagaat aatttattag ctcactaaat
      481 tgagggtaag gaaaagtaaa agcagtaaga aaaatgtctt gcattataca gcaacctttt
      541 gggaatgagt ggatggattg aataaaattt gattaagagt ggatgattta tctgtagatt
      601 attattggac agttagtctt gaagtagtct aagaattagg ttataatcag tagaagcctt
      661 gctaataatg aggaggttag tttatgtata
//
LOCUS       DAVKNL010000137          646 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVKNL010000137 DAVKNL010000000
VERSION     DAVKNL010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.137"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(21..>646)
                     /locus_tag="VW411_002133"
     CDS             complement(21..>646)
                     /locus_tag="VW411_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9794765.1"
                     /translation="IQFNDIKTSYAEYTIIGVIALLIIGQMTQVIYRVTIDKKYGLLA
                     LKLCSGVRPLYYILGMSIYSILGLIVQEIIIYIITLAFEINIAMDRFFYTVLLSIVVL
                     LFWDSLAILLTMFINDYRRRDIVIRFVLTPLGFTAPVFYLIDSAPSIVRWIGQLNPLT
                     YQLTILRNFYFKNSTTLELVFLLLTSLLVLISVSFIIPKIKLILIER"
BASE COUNT          262 a          101 c           83 g          200 t
ORIGIN      
        1 aaagttggat gacccaactt ttatctttct atcagtatca attttatctt tggtataata
       61 aaagatacag atataaggac aagtaatgat gttaacaata agaaaactaa ttccaaagtt
      121 gttgaatttt taaaataaaa gtttctcaaa atagttaatt gataagttaa gggatttaac
      181 tgaccaatcc atctcacaat actaggagca gaatctatta agtagaaaac aggagctgta
      241 aaaccaagcg gtgttagtac aaaacgtatt acaatatcac gtcttctgta atcattgata
      301 aacattgtaa gtaaaattgc aagggagtcc caaaataata aaacaacaat agataacaaa
      361 actgtataaa aaaatctatc cattgcgata tttatctcaa acgctaacgt aattatatat
      421 ataataattt cttgaactat caaccctaat atagaataga tactcatccc taaaatataa
      481 tataaaggac gaactccact gcataactta agagcaagta gcccatattt tttatctatt
      541 gtcaccctat aaataacttg agtcatctgc ccgattatca ataaagctat aacaccaatg
      601 attgtatatt ctgcataaga ggtttttata tcattaaact gaatgt
//
LOCUS       DAVKNL010000138          635 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVKNL010000138 DAVKNL010000000
VERSION     DAVKNL010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.138"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            complement(372..599)
                     /locus_tag="VW411_002134"
     CDS             complement(372..599)
                     /locus_tag="VW411_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9794766.1"
                     /translation="MSLSGGQKQRLAIASVMCKNSPFVFFDEPSSGMDYSNMIKISEL
                     INKYKTMDKIIFIVSHDIEFLNEVADEIFEL"
BASE COUNT          233 a          102 c           67 g          233 t
ORIGIN      
        1 attatatgaa aatcccatat cattctccaa ttctatattt tacctttcta aatgtataga
       61 ttaactacct aattatagca tataacgcag attcctttca atcgtatgat ttactgcatt
      121 aaattaagta aaaaaataaa ggcagtccga agactgccga tatttatctc tcatctcttt
      181 aattatggta agtaaataaa taatttccct aaagatatgg aaattattaa tactataaat
      241 acatattata aagtttataa atactgtaaa aatcctgaag ttaattttct aataaatatc
      301 aatatgtgtt agtatctttt aaatttttag acaatttact agttctatag acatgtttaa
      361 cagactctat tttacaattc aaaaatttca tctgccactt catttaaaaa ttctatatca
      421 tgggaaacaa taaaaattat tttatccatg gttttatact tattaatcag ttcagatatt
      481 tttatcatat tggaataatc cataccactt gaaggttcgt caaaaaagac aaatggagaa
      541 ttcttgcaca taacagatgc tattgcaagc ctttgctttt gccctcctga taaactcatc
      601 ggatgccttt caataaattc gtccaggcat aaatc
//
LOCUS       DAVKNL010000139          598 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVKNL010000139 DAVKNL010000000
VERSION     DAVKNL010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.139"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            132..598
                     /locus_tag="VW411_002135"
                     /pseudo
     CDS             132..598
                     /locus_tag="VW411_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
BASE COUNT          210 a          143 c          131 g          114 t
ORIGIN      
        1 ctgaagatga tactgctgct cttcaaaata aattagctac taaaaaagct gaattggaaa
       61 aaactcaaaa agaattagat gcagctctta atgagttagg ccctgatgga gatgaagaag
      121 aaactccagc gccggctcct caaccagagc aaccagctcc tgcaccaaaa ccagagcaac
      181 cagctccagc tccaaaacca gagcaaccag ctccagctcc aaaaccagag caaccagcta
      241 agccggagaa accagctgaa gagcctactc aaccagaaaa accagccact ccaaaaacag
      301 gctggaaaca agaaaacggt atgtggtatt tctacaatac tgatggttca atggcaatag
      361 gttggctcca aaacaacggt tcatggtact acctaaacgc taacggcgct atggcaacag
      421 gttgggtgaa agatggagat acctggtact atcttgaagc atcaggtgct atgaaagcaa
      481 gccaatggtt caaagtatca gataaatggt actatgtcaa cagcaatggc gctatggcga
      541 caggctggct ccaatacaat ggctcatggt actacctcaa cgctaatggt gatatggc
//
LOCUS       DAVKNL010000140          568 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVKNL010000140 DAVKNL010000000
VERSION     DAVKNL010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.140"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            <1..>568
                     /locus_tag="VW411_002136"
     CDS             <1..>568
                     /locus_tag="VW411_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU9794767.1"
                     /translation="PIRHPERLGKPNAQITYTDDEIQVAKLAGKYTTEDGYIFDPRDI
                     TSDEGDAYVTPHMTHSHWIKKDSLSEAERAAAQAYAKEKGLTPPSTDHQDSGNTEAKG
                     AEAIYNRVKAAKKVPLDRMPYNLQYTVEVKNGSLIIPHYDHYHNIKFEWFDEGLYEAP
                     KGYTLEDLLATVKYYVEHPNERPHSDNGF"
BASE COUNT          183 a          119 c          126 g          140 t
ORIGIN      
        1 ccgattcgtc atccagaacg tttaggaaaa ccaaatgcgc aaattaccta cactgatgat
       61 gagattcaag tagccaagtt ggcaggcaag tacacaacag aagacggtta tatctttgat
      121 cctcgtgata taaccagtga tgagggggat gcctatgtaa ctccacatat gacccatagc
      181 cactggatta aaaaagatag tttgtctgaa gctgagagag cggcagccca ggcttatgct
      241 aaagagaaag gtttgacccc tccttcgaca gaccatcagg attcaggaaa tactgaggca
      301 aaaggagcag aagctatcta caaccgcgtg aaagcagcta agaaggtgcc acttgatcgt
      361 atgccttaca atcttcaata tactgtagaa gtcaaaaacg gtagtttaat catacctcat
      421 tatgaccatt accataacat caaatttgag tggtttgacg aaggccttta tgaggcacct
      481 aaggggtata ctcttgagga tcttttggcg actgtcaagt actatgtcga acatccaaac
      541 gaacgtccgc attcagataa tggttttg
//
LOCUS       DAVKNL010000141          548 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00019730
            SAMN33606529-rid17942803.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVKNL010000141 DAVKNL010000000
VERSION     DAVKNL010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606529
            Sequence Read Archive: SRR23727320
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722362.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722362.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:10:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 74 of 142
            Pseudo Genes (incomplete)         :: 62 of 142
            Pseudo Genes (internal stop)      :: 40 of 142
            Pseudo Genes (multiple problems)  :: 28 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606529-rid17942803.denovo.141"
                     /strain="AUSMDU00019730"
                     /serotype="4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2018"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST205"
     gene            15..>548
                     /locus_tag="VW411_002137"
     CDS             15..>548
                     /locus_tag="VW411_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEU9794768.1"
                     /translation="MLYVGIDVAKNKHDVTALNVPGKTVLKPLTFSNNKAGFELLDLS
                     LRQLNQDCLIALENTGHYAFNLLNFLHEQGYKVYTYNPLLIKKFAKSLSLRKTKTDKK
                     DAHGIALKLLSDPNREQFQHDNRQVELKILARHIHRLKKKQSDWKVQYTRCLDIIFPE
                     LDKIVGKHSEYTYQLLTR"
BASE COUNT          182 a          124 c           84 g          158 t
ORIGIN      
        1 ctggagatac tcccatgctt tatgtgggca ttgatgtcgc taaaaataaa cacgatgtta
       61 cagccttgaa tgttccagga aaaactgttc ttaaaccact cactttttca aataataaag
      121 ctggttttga actcttagat ctgtctcttc gacagctcaa ccaagactgt ctcatcgctc
      181 ttgaaaatac cggacattat gcctttaatc tactgaactt cctgcatgaa caaggatata
      241 aagtttacac ctacaatcct ttgctcatca agaaatttgc caaatcatta tcacttcgca
      301 aaactaaaac ggataagaaa gacgctcatg gcatcgctct taaacttctt tctgacccca
      361 atcgtgaaca atttcaacac gataatcggc aagtagaact aaaaatactg gctagacata
      421 ttcatcgtct caagaaaaaa cagtctgatt ggaaagtaca atacactcgt tgtcttgata
      481 tcatctttcc tgagttggat aaaatcgttg gaaagcattc agaatatacc taccaactct
      541 tgacacgc
//
